Welcome to the 19th year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
It was productive year for all of us. During 2010, the ListServer delivered 1926 messages to over 3000 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated 460+ Gb of Email traffic and over 5.8 Million Email messages were sent out this year by my tired little server. You don't want to know how much Junk Mail and spam has been filtered out.
The complete Microscopy ListServer Archives for 2010-1993 (~ are on-line at
http://www.microscopy.com.
A couple of final reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 12, 22 -- From zaluzec-at-aaem.amc.anl.gov Sat Jan 1 09:04:44 2011 12, 22 -- Received: from znl.com ([206.69.208.20]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p01F4iF5030770 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 09:04:44 -0600 12, 22 -- Received: from localhost (localhost [127.0.0.1]) 12, 22 -- by znl.com (Postfix) with ESMTP id 52900301573 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 09:04:44 -0600 (CST) 12, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 22 -- Received: from znl.com ([127.0.0.1]) 12, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 22 -- with ESMTP id Qzo4tuqkHH8m for {microscopy-at-microscopy.com} ; 12, 22 -- Sat, 1 Jan 2011 09:04:41 -0600 (CST) 12, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 12, 22 -- by znl.com (Postfix) with ESMTPA id 07908301563 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 09:04:40 -0600 (CST) 12, 22 -- Mime-Version: 1.0 12, 22 -- Message-Id: {p06240800c944eb5835ba-at-[206.69.208.22]} 12, 22 -- Date: Sat, 1 Jan 2011 09:04:39 -0600 12, 22 -- To: microscopy-at-microscopy.com 12, 22 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 12, 22 -- Subject: Administrivia: Happy New Year 2011 12, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
-----Original Message----- X-from: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY-at-LISTS.UMN.EDU] On Behalf Of Teng-Leong Chew Sent: Saturday, January 01, 2011 3:16 AM To: CONFOCALMICROSCOPY-at-LISTS.UMN.EDU
Thank you, Nestor, and a Happy New Year 2011 to you, too.
My I suggest that all participants at this list server silently give Nestor a big hand for keeping it alive and a valuable resource to everybody (while keeping the spam to a minimum)?
Thanks, Nestor.
Mike --- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Saturday, January 01, 2011 8:15 AM To: mike.bode-at-resaltatech.com
Happy New Year Colleagues;
Welcome to the 19th year of operation of the Microscopy ListServer a free service to the world wide microscopy community, sponsored jointly by your Friendly Neighborhood SysOp and the Microscopy Society of America.
It was productive year for all of us. During 2010, the ListServer delivered 1926 messages to over 3000 subscribers around the world, with minimal hassels (that I know about). For those of you that are statistics junkies this year you generated 460+ Gb of Email traffic and over 5.8 Million Email messages were sent out this year by my tired little server. You don't want to know how much Junk Mail and spam has been filtered out.
The complete Microscopy ListServer Archives for 2010-1993 (~ are on-line at
http://www.microscopy.com.
A couple of final reminders:
If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE your Email address from the listserver. The out-of-office / on-vacation autoreply messages are a real nuisance to posters.
As always if you have questions about suitability of postings or are having problems, feel free to contact me at (zaluzec-at-microscopy.com)
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 12, 22 -- From zaluzec-at-aaem.amc.anl.gov Sat Jan 1 09:04:44 2011 12, 22 -- Received: from znl.com ([206.69.208.20]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p01F4iF5030770 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 09:04:44 -0600 12, 22 -- Received: from localhost (localhost [127.0.0.1]) 12, 22 -- by znl.com (Postfix) with ESMTP id 52900301573 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 09:04:44 -0600 (CST) 12, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 22 -- Received: from znl.com ([127.0.0.1]) 12, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 22 -- with ESMTP id Qzo4tuqkHH8m for {microscopy-at-microscopy.com} ; 12, 22 -- Sat, 1 Jan 2011 09:04:41 -0600 (CST) 12, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) 12, 22 -- by znl.com (Postfix) with ESMTPA id 07908301563 12, 22 -- for {microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 09:04:40 -0600 (CST) 12, 22 -- Mime-Version: 1.0 12, 22 -- Message-Id: {p06240800c944eb5835ba-at-[206.69.208.22]} 12, 22 -- Date: Sat, 1 Jan 2011 09:04:39 -0600 12, 22 -- To: microscopy-at-microscopy.com 12, 22 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 12, 22 -- Subject: Administrivia: Happy New Year 2011 12, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 28, 31 -- From mike.bode-at-resaltatech.com Sat Jan 1 12:45:19 2011 28, 31 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 28, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p01IjJ2m012505 28, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 1 Jan 2011 12:45:19 -0600 28, 31 -- Received: from c-98-245-67-110.hsd1.co.comcast.net ([98.245.67.110]:20843 helo=resdtpden01) 28, 31 -- by web38.justhost.com with esmtpa (Exim 4.69) 28, 31 -- (envelope-from {mike.bode-at-resaltatech.com} ) 28, 31 -- id 1PZ6S8-0006pf-4T 28, 31 -- for Microscopy-at-microscopy.com; Sat, 01 Jan 2011 12:45:20 -0600 28, 31 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 28, 31 -- To: {Microscopy-at-microscopy.com} 28, 31 -- References: {201101011514.p01FEgak012697-at-ns.microscopy.com} 28, 31 -- In-Reply-To: {201101011514.p01FEgak012697-at-ns.microscopy.com} 28, 31 -- Subject: RE: [Microscopy] Administrivia: Happy New Year 2011 28, 31 -- Date: Sat, 1 Jan 2011 11:45:10 -0700 28, 31 -- Message-ID: {000901cba9e4$0663c2f0$132b48d0$-at-bode-at-resaltatech.com} 28, 31 -- MIME-Version: 1.0 28, 31 -- Content-Type: text/plain; 28, 31 -- charset="us-ascii" 28, 31 -- Content-Transfer-Encoding: 7bit 28, 31 -- X-Mailer: Microsoft Office Outlook 12.0 28, 31 -- Thread-Index: Acupxp4FVAM4V69bTlqgLFGuT2YV0AAHIhFA 28, 31 -- Content-Language: en-us 28, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 28, 31 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 28, 31 -- X-AntiAbuse: Original Domain - microscopy.com 28, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 28, 31 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 28, 31 -- X-Source: 28, 31 -- X-Source-Args: 28, 31 -- X-Source-Dir: ==============================End of - Headers==============================
I was just working with a MetroCal (I think that's how it's spelled) calibration wafer in an SEM, and I noticed that there is quite a bit of dust on it. Is there a safe and effective way to clean a printed silicon wafer which won't do any damage to it?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 30 -- From kraftpiano-at-gmail.com Mon Jan 3 14:34:42 2011 4, 30 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03KYgBl011394 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 14:34:42 -0600 4, 30 -- Received: by bwz16 with SMTP id 16so16667740bwz.0 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:mime-version:received:received:date:message-id 4, 30 -- :subject:from:to:content-type; 4, 30 -- bh=qHwya0PLKzzf5arg1ByIRcBmNZgGAxILE37Z1eUGd7w=; 4, 30 -- b=ADHwB7MjWoVvu458S9KJNAJWWfwT9aGeIxTuwjwPXaSchhjeiyTlcswrVqOQ+dm3NT 4, 30 -- EsIaLLkiRHtgxc0vnSK2GQo4Z9U7vVlX4zwOfY5qvYXSD/meUYt0CuLdoTn0o8NWuRJi 4, 30 -- hvXzm/kvRE918r4zbu5auzokV1PYoUjMPS3UU= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 30 -- b=FQnXYcMpueHSMi4XvU2Nlrxdxci+SkGLwREUbM/xnC75MnKqhuMfSBMIbNVeZD5taM 4, 30 -- BM5ZCqxz5DJ1qCWDHWrmxs+fBnDw//SCoTXtIQmCzolpcq3tWcI8Ewe8Yvgh0RQMI4nm 4, 30 -- biBNEKqkU69U/9r+rrXK/wf1q4fVKhRZeyZu4= 4, 30 -- MIME-Version: 1.0 4, 30 -- Received: by 10.204.57.204 with SMTP id d12mr16124775bkh.69.1294086881598; 4, 30 -- Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Received: by 10.204.60.203 with HTTP; Mon, 3 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Date: Mon, 3 Jan 2011 15:34:41 -0500 4, 30 -- Message-ID: {AANLkTik+W=37hfEX7EozPoA+VmZioTm2yurMO9XuD-bf-at-mail.gmail.com} 4, 30 -- Subject: Cleaning silicon wafers 4, 30 -- From: Justin Kraft {kraftpiano-at-gmail.com} 4, 30 -- To: microscopy-at-microscopy.com 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Justin, I looked on Metrocal's website and didn't find anything on silicon. Is it perhaps from Geller? Anyway, if you can find the actual manufacturer, there should be cleaning instructions available online.
If not, items on silicon wafers are actually pretty hardy if you don't physically scratch them. A duster, for starters, may take care of the dust. Is it mounted on a stub? Do you know what it's mounted with? Organic solvents (alcohols, acetone) shouldn't hurt the silicon or metallization (usually Al, sometimes Au) followed by a duster. The mounting material is more likely to present a problem than the wafer itself.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 03, 2011 3:38 PM To: kenconverse-at-qualityimages.biz
Greetings everyone, and happy new year!
I was just working with a MetroCal (I think that's how it's spelled) calibration wafer in an SEM, and I noticed that there is quite a bit of dust on it. Is there a safe and effective way to clean a printed silicon wafer which won't do any damage to it?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 30 -- From kraftpiano-at-gmail.com Mon Jan 3 14:34:42 2011 4, 30 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03KYgBl011394 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 14:34:42 -0600 4, 30 -- Received: by bwz16 with SMTP id 16so16667740bwz.0 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:mime-version:received:received:date:message-id 4, 30 -- :subject:from:to:content-type; 4, 30 -- bh=qHwya0PLKzzf5arg1ByIRcBmNZgGAxILE37Z1eUGd7w=; 4, 30 -- b=ADHwB7MjWoVvu458S9KJNAJWWfwT9aGeIxTuwjwPXaSchhjeiyTlcswrVqOQ+dm3NT 4, 30 -- EsIaLLkiRHtgxc0vnSK2GQo4Z9U7vVlX4zwOfY5qvYXSD/meUYt0CuLdoTn0o8NWuRJi 4, 30 -- hvXzm/kvRE918r4zbu5auzokV1PYoUjMPS3UU= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 30 -- b=FQnXYcMpueHSMi4XvU2Nlrxdxci+SkGLwREUbM/xnC75MnKqhuMfSBMIbNVeZD5taM 4, 30 -- BM5ZCqxz5DJ1qCWDHWrmxs+fBnDw//SCoTXtIQmCzolpcq3tWcI8Ewe8Yvgh0RQMI4nm 4, 30 -- biBNEKqkU69U/9r+rrXK/wf1q4fVKhRZeyZu4= 4, 30 -- MIME-Version: 1.0 4, 30 -- Received: by 10.204.57.204 with SMTP id d12mr16124775bkh.69.1294086881598; 4, 30 -- Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Received: by 10.204.60.203 with HTTP; Mon, 3 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Date: Mon, 3 Jan 2011 15:34:41 -0500 4, 30 -- Message-ID: {AANLkTik+W=37hfEX7EozPoA+VmZioTm2yurMO9XuD-bf-at-mail.gmail.com} 4, 30 -- Subject: Cleaning silicon wafers 4, 30 -- From: Justin Kraft {kraftpiano-at-gmail.com} 4, 30 -- To: microscopy-at-microscopy.com 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 28 -- From kenconverse-at-qualityimages.biz Mon Jan 3 16:41:56 2011 18, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03Mfuvf031739 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 16:41:56 -0600 18, 28 -- X-Authority-Analysis: v=1.1 cv=uESSSoDEku2quKX/oFXS2Smn5+55LTFcWFr5T5T8nFs= c=1 sm=0 a=EPkJnWdmquUA:10 a=kj9zAlcOel0A:10 a=o/BqynnMLxMJiB4/byRFHg==:17 a=pGLkceISAAAA:8 a=Zx37jsudAAAA:8 a=1XWaLZrsAAAA:8 a=ncwpZeBwXMnrfOMQ3bQA:9 a=US01wcXEiGvzWnChvZQA:7 a=1H2jpXTeLfj6DHcIsEfKllJOVbMA:4 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=fM1NKZWFy18A:10 a=RyjLG6jaqdoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=o/BqynnMLxMJiB4/byRFHg==:117 18, 28 -- X-Cloudmark-Score: 0 18, 28 -- X-Originating-IP: 72.227.97.248 18, 28 -- Received: from [72.227.97.248] ([72.227.97.248:4582] helo=Ken) 18, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 18, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 18, 28 -- id 83/2A-07087-4B0522D4; Mon, 03 Jan 2011 22:41:56 +0000 18, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 28 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 18, 28 -- Subject: RE: [Microscopy] Cleaning silicon wafers 18, 28 -- Date: Mon, 3 Jan 2011 17:41:48 -0500 18, 28 -- Message-ID: {9F9035F8BFEB4E3FB7E478F4EC892D5C-at-Ken} 18, 28 -- MIME-Version: 1.0 18, 28 -- Content-Type: text/plain; 18, 28 -- charset="us-ascii" 18, 28 -- X-Priority: 3 (Normal) 18, 28 -- X-MSMail-Priority: Normal 18, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 18, 28 -- In-Reply-To: {201101032038.p03Kc1CM015029-at-ns.microscopy.com} 18, 28 -- Importance: Normal 18, 28 -- Thread-Index: Acurhhz1BXcIliXoQuu6YgNFf4AoAAAECPwg 18, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p03Mfuvf031739 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pitrone-at-mpi-cbg.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pitrone-at-mpi-cbg.de Name: Peter Pitrone
Organization: Max Planck Institute for Molecular Cell Biology and Genetics
Title-Subject: [Filtered] REMINDER: Microscopist/Imaging-specialist position/s open immediately: MPI-CBG Light Microscopy Facility in Dresden, Germany
Message: Dear friends of microscopy and imaging,
We are very actively searching for 2 microscopists / imaging specialists to join our light microscopy facility team with immediate effect.
The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) in Dresden is seeking motivated Microscopists/Imaging Specialists, to work in its Light Microscopy Facility (LMF).
The LMF is a large core facility enabling different aspects of MPI-CBG researcherís imaging projects including: design of imaging experiments, choice of and training on suitable imaging systems, and image processing, visualisation and analysis. The LMF is part of a network of core imaging facilities on the Dresden Biopolis campus (see https://ifn.mpi-cbg.de.) Light microscopy is one of the key methods of the MPI-CBG. The LMF provides more than 20 advanced imaging systems including laser scanning confocal microscopy, two-photon microscopy, wide-field microscopy, TIRF, SPIM and PALM/STORM. A super resolution structured illumination (SIM) system will be installed in 2011. The LMF team supports approximately 270 users from over 35 countries. More than 40 000 hours of instrument time is booked annually. The working language of the MPI-CBG is English.
Requirements: We are seeking 1-2 candidates who are proactive and service-oriented team players with excellent interpersonal and organizational skills and who enjoy helping people. They should have a first degree (B.Sc.) and preferably also a doctorate degree (Ph.D.) in biology or physics/optics, be fluent in English and be ready to help scientists with both trivial and advanced aspects of light microscopy imaging projects. Experience in basic as well as advanced light microscopy is required and some experience with image analysis would be a strong plus. The successful candidate(s) should be ready to serve in a dynamic international multi-user environment whereby four basic areas of service must be covered: a) scientific support of imaging projects, b) implementation of new imaging technologies, c) maintenance and service of current imaging systems and d) training and teaching light microscopy. Ideally, the candidate(s) should have experience working in an imaging facility.
The positions are available immediately. The initial contract is for 2 years. Compensation will depend on the qualifications and experience of the candidate. The Max Planck Society is committed to employ more disabled persons. The application of disabled persons is strongly encouraged. The Max Planck Society is committed to increase the number of female scientists. Women are particularly encouraged to apply.
For informal inquiries, please contact the leader of the LMF, Dr. Jan Peychl, email: peychl-at-mpi-cbg.de
If you wish to apply for this position, please send your CV, letter of application/motivation and the contact information of three referees to:
Max Planck Institute of Molecular Cell Biology and Genetics Code: 2010-LMF-4100 Pfotenhauerstr. 108 01307 Dresden Germany or by email: 2010-LMF-4100-at-mpi-cbg.de
The University of Wisconsin Eau Claire is seeking applicants for an Analytical Scientist position in the Materials Science Center, an interdisciplinary analytical facility, specializing in materials characterization. This is a full-time professional academic staff position beginning July 1st 2011. Potential applicants may obtain a complete position description and application requirements at http://www.uwec.edu/Matsci/position.html. Women, minorities, individuals with disabilities and veterans are encouraged to apply. Criminal background checks are required prior to employment. Review of completed applications will commence January 14th, 2011 and continue until the position is filled.
The scientist will help manage instrumentation associated with the Materials Science Center, an interdisciplinary analytical facility that incorporates an array of state-of-the-art instrumentation specializing in materials characterization and surface science. Instrumentation within the Center includes a 200 keV Transmission Electron Microscope, Scanning Electron Microscopes with Energy Dispersive X-ray Microanalyzer, a High Resolution Inductively Coupled Plasma - Mass Spectrometer, X-ray Photoelectron Spectrometer, Scanning Tunneling Microscope, 2 X-ray Fluorescence Spectrometers, X-ray Diffractometer, Atomic Force Microscopes, Scanning Auger Nanoprobe and a Micro-FTIR. The scientist will assist with the development of the Electron Microscopy facility, and will be involved in all aspects of technique development, instrument control and maintenance, and other aspects of running a professional laboratory. The scientist will be responsible for instrument maintenance, repair, sample preparation and analysis in other high vacuum applications, such as XRD, XRF, XPS and STM utilized by faculty associated with the Materials Science Center. Precise responsibilities will vary according to research priorities within the Materials Science Center. The scientist is encouraged to participate in collaborative research with faculty, undergraduates and local industry, as well as other scholarly activities. The scientist may be asked to teach some instrument specific courses.
----------------------------------------------------------------------------------- Dr. Doug Dunham Director, Materials Science Center University of Wisconsin-Eau Claire Phillips Hall, Suite 177 105 Garfield Avenue Eau Claire, WI 54702-4004 tel: 715-836-5312 fax: 715-836-3556
==============================Original Headers============================== 9, 32 -- From prvs=498578EF40=DUNHAMDJ-at-uwec.edu Tue Jan 4 11:24:04 2011 9, 32 -- Received: from EX2010-EDGE1.uwec.edu (ex2010-edge1.uwec.edu [137.28.1.209]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p04HO2HI003413 9, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Jan 2011 11:24:03 -0600 9, 32 -- Received: from EX2010-CASHUB1.uwec.edu (137.28.3.34) by EX2010-EDGE1.uwec.edu 9, 32 -- (137.28.1.209) with Microsoft SMTP Server (TLS) id 14.1.270.1; Tue, 4 Jan 9, 32 -- 2011 11:23:49 -0600 9, 32 -- Received: from EX2010-MBX1.uwec.edu ([fe80::d420:b791:b600:11aa]) by 9, 32 -- EX2010-CASHUB1.uwec.edu ([fe80::1ce5:96ef:4fef:7a90%23]) with mapi id 9, 32 -- 14.01.0270.001; Tue, 4 Jan 2011 11:24:01 -0600 9, 32 -- From: "Dunham, Douglas J." {DUNHAMDJ-at-uwec.edu} 9, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 32 -- Subject: Available Position: Analytical Scientist 9, 32 -- Thread-Topic: Available Position: Analytical Scientist 9, 32 -- Thread-Index: AQHLrDQsYRYWsHXo6UGuZpYGK9XJEA== 9, 32 -- Date: Tue, 4 Jan 2011 17:24:00 +0000 9, 32 -- Message-ID: {9802B08AEF25764489DDDF11CB2C9B640CAF270A-at-EX2010-MBX1.uwec.edu} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- x-cr-hashedpuzzle: BcJ+ BhKY C/Yj DGCY ESYW EUdS Elms Fou1 HW3F Hcrn HkL7 9, 32 -- H+1X IDKZ IVZs I1yC 9, 32 -- JBvp;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{98245CD3-83A9-4BC8-9FF8-6E3E71B3275B};ZAB1AG4AaABhAG0AZABqAEAAdQB3AGUAYwAuAGUAZAB1AA==;Tue, 9, 32 -- 04 Jan 2011 17:23:58 9, 32 -- GMT;QQB2AGEAaQBsAGEAYgBsAGUAIABQAG8AcwBpAHQAaQBvAG4AOgAgACAAQQBuAGEAbAB5AHQAaQBjAGEAbAAgAFMAYwBpAGUAbgB0AGkAcwB0AA== 9, 32 -- x-cr-puzzleid: {98245CD3-83A9-4BC8-9FF8-6E3E71B3275B} 9, 32 -- x-originating-ip: [137.28.27.128] 9, 32 -- Content-Type: text/plain; charset="iso-8859-1" 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p04HO2HI003413 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both aheath-at-ufl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: aheath-at-ufl.edu Name: Ann Heatherington
Organization: Dept. of Geological Sciences, University of Florida
Title-Subject: [Filtered] SEM-- Quantomix Wet SEM capsules?
Message: Hello-- Does anyone have any experience with the Wet SEM capsules by Quantomix?
We have a Zeiss EVO MA 10 SEM and every once in a while have someone interested in looking at a wet sample (gels, sediments, etc.) This looked like a possible economical way of gaining wet sample analysis capability, and I would like to hear opinions from someone who has actually used it. The vendor will not supply a customer list.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kintzjd-at-rocketmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kintzjd-at-rocketmail.com Name: Joe Kintz
Organization: Stress Engineering Services, Inc.
Title-Subject: [Filtered] Repair or replace EDS detector?
Message: Our EDAX-brand Sapphire Si(Li) EDS detector probably has a crack or hole in its super ultra thin window and will probably have to be sent to EDAX for repair. Is this a good time to consider upgrading to a silicon drift detector? Using liquid nitrogen is not a problem for us but, if we can improve EDS performance at a reasonable price compared to repairing our existing Si(Li) detector, this would seem to make sense. We're also using EDAX's EBSD system, so we'd like to continue using their Genesis EDS software.
If this would be a good time to upgrade, what is the concensus about the silicon drift detectors (SDD)? Are they better across the board than the Si(Li) detectors, or would we be giving up some aspect of performance? Who makes the best SDD's that will work with EDAX EDS systems?
I would agree with Ken on the use of a compressed gas as a first attempt at cleaning your wafer, but I would actually avoid those tetrafluoroethane dusters. It's been my experience that they can leave a film on the surface of the wafer. If you have a spare piece of polished silicon wafer hanging around you could test the duster on it to see if it leaves a film. I prefer using high purity dry nitrogen from a gas cylinder, but I have also used the compressed CO2 dusters you can find at office supply stores with good results.
Since it looks like this wafer is etched silicon, you should be safe using a solvent if there is anything stuck on it, but I would stress that you should contact the manufacturer (Metroboost*) to make sure that they don't have some kind of a coating on it or something that would be damaged by a particular solvent. A good general choice to start would actually be deionized water, since the etch process to make the wafer probably employed that anyway. The procedure that we used during my semiconductor courses was to rinse the chip or wafer with DI water and then blow-dry it with dry nitrogen. It helps if you hold the wafer near vertical with one end resting on a lint-free paper towel and then blow the residual solvent towards the towel starting from the top of the wafer and working your way down. This generally leaves a clean, lint free, surface.
Hope that helps!
Mike Anderson
*Comments made in this correspondence represent the opinion of the author and should not be construed as endorsement or recommendation by NIST. Likewise, any mention of commercial products does not imply recommendation or endorsement by NIST.
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Monday, January 03, 2011 5:51 PM To: Anderson, Michael
Justin, I looked on Metrocal's website and didn't find anything on silicon. Is it perhaps from Geller? Anyway, if you can find the actual manufacturer, there should be cleaning instructions available online.
If not, items on silicon wafers are actually pretty hardy if you don't physically scratch them. A duster, for starters, may take care of the dust. Is it mounted on a stub? Do you know what it's mounted with? Organic solvents (alcohols, acetone) shouldn't hurt the silicon or metallization (usually Al, sometimes Au) followed by a duster. The mounting material is more likely to present a problem than the wafer itself.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] Sent: Monday, January 03, 2011 3:38 PM To: kenconverse-at-qualityimages.biz
Greetings everyone, and happy new year!
I was just working with a MetroCal (I think that's how it's spelled) calibration wafer in an SEM, and I noticed that there is quite a bit of dust on it. Is there a safe and effective way to clean a printed silicon wafer which won't do any damage to it?
--Justin A. Kraft
-- "America believes in education; the average professor earns more money in a year than a professional athlete earns in a whole week." Evan Esar
==============================Original Headers============================== 4, 30 -- From kraftpiano-at-gmail.com Mon Jan 3 14:34:42 2011 4, 30 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03KYgBl011394 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 14:34:42 -0600 4, 30 -- Received: by bwz16 with SMTP id 16so16667740bwz.0 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:mime-version:received:received:date:message-id 4, 30 -- :subject:from:to:content-type; 4, 30 -- bh=qHwya0PLKzzf5arg1ByIRcBmNZgGAxILE37Z1eUGd7w=; 4, 30 -- b=ADHwB7MjWoVvu458S9KJNAJWWfwT9aGeIxTuwjwPXaSchhjeiyTlcswrVqOQ+dm3NT 4, 30 -- EsIaLLkiRHtgxc0vnSK2GQo4Z9U7vVlX4zwOfY5qvYXSD/meUYt0CuLdoTn0o8NWuRJi 4, 30 -- hvXzm/kvRE918r4zbu5auzokV1PYoUjMPS3UU= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 30 -- b=FQnXYcMpueHSMi4XvU2Nlrxdxci+SkGLwREUbM/xnC75MnKqhuMfSBMIbNVeZD5taM 4, 30 -- BM5ZCqxz5DJ1qCWDHWrmxs+fBnDw//SCoTXtIQmCzolpcq3tWcI8Ewe8Yvgh0RQMI4nm 4, 30 -- biBNEKqkU69U/9r+rrXK/wf1q4fVKhRZeyZu4= 4, 30 -- MIME-Version: 1.0 4, 30 -- Received: by 10.204.57.204 with SMTP id d12mr16124775bkh.69.1294086881598; 4, 30 -- Mon, 03 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Received: by 10.204.60.203 with HTTP; Mon, 3 Jan 2011 12:34:41 -0800 (PST) 4, 30 -- Date: Mon, 3 Jan 2011 15:34:41 -0500 4, 30 -- Message-ID: {AANLkTik+W=37hfEX7EozPoA+VmZioTm2yurMO9XuD-bf-at-mail.gmail.com} 4, 30 -- Subject: Cleaning silicon wafers 4, 30 -- From: Justin Kraft {kraftpiano-at-gmail.com} 4, 30 -- To: microscopy-at-microscopy.com 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 28 -- From kenconverse-at-qualityimages.biz Mon Jan 3 16:41:56 2011 18, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p03Mfuvf031739 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 3 Jan 2011 16:41:56 -0600 18, 28 -- X-Authority-Analysis: v=1.1 cv=uESSSoDEku2quKX/oFXS2Smn5+55LTFcWFr5T5T8nFs= c=1 sm=0 a=EPkJnWdmquUA:10 a=kj9zAlcOel0A:10 a=o/BqynnMLxMJiB4/byRFHg==:17 a=pGLkceISAAAA:8 a=Zx37jsudAAAA:8 a=1XWaLZrsAAAA:8 a=ncwpZeBwXMnrfOMQ3bQA:9 a=US01wcXEiGvzWnChvZQA:7 a=1H2jpXTeLfj6DHcIsEfKllJOVbMA:4 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=fM1NKZWFy18A:10 a=RyjLG6jaqdoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=o/BqynnMLxMJiB4/byRFHg==:117 18, 28 -- X-Cloudmark-Score: 0 18, 28 -- X-Originating-IP: 72.227.97.248 18, 28 -- Received: from [72.227.97.248] ([72.227.97.248:4582] helo=Ken) 18, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 18, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 18, 28 -- id 83/2A-07087-4B0522D4; Mon, 03 Jan 2011 22:41:56 +0000 18, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 18, 28 -- To: {kraftpiano-at-gmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 18, 28 -- Subject: RE: [Microscopy] Cleaning silicon wafers 18, 28 -- Date: Mon, 3 Jan 2011 17:41:48 -0500 18, 28 -- Message-ID: {9F9035F8BFEB4E3FB7E478F4EC892D5C-at-Ken} 18, 28 -- MIME-Version: 1.0 18, 28 -- Content-Type: text/plain; 18, 28 -- charset="us-ascii" 18, 28 -- X-Priority: 3 (Normal) 18, 28 -- X-MSMail-Priority: Normal 18, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 18, 28 -- In-Reply-To: {201101032038.p03Kc1CM015029-at-ns.microscopy.com} 18, 28 -- Importance: Normal 18, 28 -- Thread-Index: Acurhhz1BXcIliXoQuu6YgNFf4AoAAAECPwg 18, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p03Mfuvf031739 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 28 -- From michael.anderson-at-nist.gov Wed Jan 5 09:17:09 2011 30, 28 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 30, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05FH8nR024260 30, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 09:17:09 -0600 30, 28 -- Received: from WSXGHUB2.xchange.nist.gov (wsxghub2.nist.gov [129.6.18.19]) 30, 28 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id p05FH4c9030458 30, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 10:17:04 -0500 30, 28 -- Received: from MBCLUSTER.xchange.nist.gov ([fe80::d479:3188:aec0:cb66]) by 30, 28 -- WSXGHUB2.xchange.nist.gov ([129.6.18.19]) with mapi; Wed, 5 Jan 2011 10:16:53 30, 28 -- -0500 30, 28 -- From: "Anderson, Michael" {michael.anderson-at-nist.gov} 30, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 30, 28 -- Date: Wed, 5 Jan 2011 10:16:59 -0500 30, 28 -- Subject: RE: [Microscopy] RE: Cleaning silicon wafers 30, 28 -- Thread-Topic: [Microscopy] RE: Cleaning silicon wafers 30, 28 -- Thread-Index: AcurmKiv9OavxWSyRr27x/LZnO5vWABTZMMg 30, 28 -- Message-ID: {7E80450DE7474E4881E405C742AC0DEA11DA4A75D2-at-MBCLUSTER.xchange.nist.gov} 30, 28 -- References: {201101032250.p03Mok37014851-at-ns.microscopy.com} 30, 28 -- In-Reply-To: {201101032250.p03Mok37014851-at-ns.microscopy.com} 30, 28 -- Accept-Language: en-US 30, 28 -- Content-Language: en-US 30, 28 -- X-MS-Has-Attach: 30, 28 -- X-MS-TNEF-Correlator: 30, 28 -- acceptlanguage: en-US 30, 28 -- Content-Type: text/plain; charset="us-ascii" 30, 28 -- MIME-Version: 1.0 30, 28 -- X-NIST-MailScanner: Found to be clean 30, 28 -- X-NIST-MailScanner-From: michael.anderson-at-nist.gov ==============================End of - Headers==============================
--- On Wed, 1/5/11, michael.anderson-at-nist.gov {michael.anderson-at-nist.gov} wrote:
} From: michael.anderson-at-nist.gov {michael.anderson-at-nist.gov} } Subject: [Microscopy] Cleaning silicon wafers } To: mcintoshgreece-at-yahoo.com } Date: Wednesday, January 5, 2011, 10:23 AM } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } } I would agree with Ken on the use of a compressed gas as a } first attempt at cleaning your wafer, but I would actually } avoid those tetrafluoroethane dusters. It's been my } experience that they can leave a film on the surface of the } wafer. If you have a spare piece of polished silicon } wafer hanging around you could test the duster on it to see } if it leaves a film. I prefer using high purity dry } nitrogen from a gas cylinder, but I have also used the } compressed CO2 dusters you can find at office supply stores } with good results. } } Since it looks like this wafer is etched silicon, you } should be safe using a solvent if there is anything stuck on } it, but I would stress that you should contact the } manufacturer (Metroboost*) to make sure that they don't have } some kind of a coating on it or something that would be } damaged by a particular solvent. A good general choice to } start would actually be deionized water, since the etch } process to make the wafer probably employed that } anyway. The procedure that we used during my } semiconductor courses was to rinse the chip or wafer with DI } water and then blow-dry it with dry nitrogen. It helps } if you hold the wafer near vertical with one end resting on } a lint-free paper towel and then blow the residual solvent } towards the towel starting from the top of the wafer and } working your way down. This generally leaves a clean, } lint free, surface. } } Hope that helps! } } Mike Anderson
} Justin, } I looked on Metrocal's website and didn't find anything on } silicon. Is it } perhaps from Geller? Anyway, if you can find the } actual manufacturer, there } should be cleaning instructions available online. } } If not, items on silicon wafers are actually pretty hardy } if you don't } physically scratch them. A duster, for starters, may } take care of the dust. } Is it mounted on a stub? Do you know what it's } mounted with? Organic } solvents (alcohols, acetone) shouldn't hurt the silicon or } metallization } (usually Al, sometimes Au) followed by a duster. The } mounting material is } more likely to present a problem than the wafer itself. } } Ken Converse } owner } } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz }
} Greetings everyone, and happy new year! } } I was just working with a MetroCal (I think that's how it's } spelled) } calibration wafer in an SEM, and I noticed that there is } quite a bit } of dust on it. Is there a safe and effective way to } clean a printed } silicon wafer which won't do any damage to it? } } --Justin A. Kraft }
==============================Original Headers============================== 15, 29 -- From mcintoshgreece-at-yahoo.com Wed Jan 5 13:44:50 2011 15, 29 -- Received: from web34208.mail.mud.yahoo.com (web34208.mail.mud.yahoo.com [66.163.178.123]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p05Jiop9019400 15, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 13:44:50 -0600 15, 29 -- Received: (qmail 58578 invoked by uid 60001); 5 Jan 2011 19:44:50 -0000 15, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1294256689; bh=61+1aoTem767+gCJaDrdPFAc01I4kpdtiQgZaMzQf90=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=WDGnNaIuatc5cJ/qIT9BMy4C/6CTTBIbKYmQLZJhLjBHoDCzw3XRrnH0ucZlNYUPPgdcfY37JJv+2RkYbJXxdstbbF4k+Fo8beFAqangFeiG7QmgrLCVjTEq4QNd2eOwGV/Q3tMuMIT/zDxH3diPpAe0E7podXHgYnfycAf7WYs= 15, 29 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 29 -- s=s1024; d=yahoo.com; 15, 29 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 15, 29 -- b=cnOn6JYvIhY0uvgciZnvScxfjbgI1HapnjQnGehj6xjIGshnzZcX/09mp+SftlFf1FbIiT61lYMbNTLYy0aUoGYkKcGbbVZBavN80IkYE6m8SDyJM8LV3ljkTe3m+F7JBANV/6V0zaJHGWlmcABqvXRm+QuqWedrsbgszcC/JkI=; 15, 29 -- Message-ID: {986129.57893.qm-at-web34208.mail.mud.yahoo.com} 15, 29 -- X-YMail-OSG: DeLCwH0VM1lvUW9QtTusQPfkrdKb0Yn7sJUGbGvQxTyVokr 15, 29 -- t4TcJxpdzUFROi9maci_5lSXleN3_iV_9GEJFh0pS7XrJ4PNrcaHEtS59fB6 15, 29 -- 7NQk9BUkKJqMW5V4cYmUGelwjmqzw.OArP_3aMPx_xj.IPexjls9ahDx15P8 15, 29 -- cAo_hcf4.UgBo3boQg4RTne6AGHEHTecGs026YO2qajCxyGD5iNz6.SJXhLF 15, 29 -- pvFbNPr6FE48Z4_5xc_yQZ2sDmFIFt.MAtXfkr4XUTDcj.DQi_T6IlCVSyWf 15, 29 -- Sj2oi9Q1wWuQrmkadSqNTLKi1IWyml5eQGZvdUmIeNNwYENJIrElO1yzVNDl 15, 29 -- DJbZ6MUW7m20pUk.hOODaCVbA.HHG_TsmkmRbS8OGtMkAz5cYoHDQWWVld5e 15, 29 -- lt8ynKwoJzRCc 15, 29 -- Received: from [198.82.148.30] by web34208.mail.mud.yahoo.com via HTTP; Wed, 05 Jan 2011 11:44:49 PST 15, 29 -- X-Mailer: YahooMailClassic/11.4.20 YahooMailWebService/0.8.107.285259 15, 29 -- Date: Wed, 5 Jan 2011 11:44:49 -0800 (PST) 15, 29 -- From: John McIntosh {mcintoshgreece-at-yahoo.com} 15, 29 -- Subject: Use Tape: Cleaning silicon wafers 15, 29 -- To: Microscopy-at-microscopy.com 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Type: text/plain; charset=iso-8859-1 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05Jiop9019400 ==============================End of - Headers==============================
Check this product as well: http://www.vatran.com/dryice.html
-- Milen Shishkov, Ph.D. Wellman Center for Photomedicine Massachusetts General Hospital Harvard Medical School
Address: MGH BAR 712 50 Blossom St. Boston, MA 02114 Phone: (617) 726 1589 Fax: (617) 643 9208
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
==============================Original Headers============================== 9, 21 -- From SHISHKOV-at-HELIX.MGH.HARVARD.EDU Wed Jan 5 14:03:24 2011 9, 21 -- Received: from phsmgmx12.partners.org (phsmgmx12.partners.org [155.52.251.71]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05K3OBC005841 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 14:03:24 -0600 9, 21 -- X-IronPort-Anti-Spam-Filtered: true 9, 21 -- X-IronPort-Anti-Spam-Result: Aq8RAEVdJE2Et4ImgWdsb2JhbACWB4YRiAMVAQEWIiS5MoYDAoMMgj4EhGiGIoMfBg 9, 21 -- Received: from phsxcon5.partners.org ([132.183.130.38]) 9, 21 -- by phsmgmx12-out.partners.org with ESMTP; 05 Jan 2011 15:03:24 -0500 9, 21 -- Received: from [132.183.44.148] ([132.183.44.148]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.4675); 9, 21 -- Wed, 5 Jan 2011 15:03:24 -0500 9, 21 -- Message-ID: {4D24CE8B.2050900-at-helix.mgh.harvard.edu} 9, 21 -- Date: Wed, 05 Jan 2011 15:03:23 -0500 9, 21 -- From: Milen Shishkov {shishkov-at-HELIX.MGH.HARVARD.EDU} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.8) Gecko/20100802 Lightning/1.0b2 Thunderbird/3.1.2 9, 21 -- MIME-Version: 1.0 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- Subject: RE: Cleaning silicon wafers 9, 21 -- X-OriginalArrivalTime: 05 Jan 2011 20:03:24.0119 (UTC) FILETIME=[9BDDF670:01CBAD13] 9, 21 -- Content-Type: text/plain; charset="iso-8859-1"; format="flowed" 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05K3OBC005841 ==============================End of - Headers==============================
We have a CPD from tousimis (815 AutoSamDri, Series A)
Pricey, but automatic and based on PhysChem. As tousimis makes big ones for complete mems wafers, plain silicon chips (i.e. 5 x 5mm) are not a problem. Biggest problem is, once retrieved in open lab, how does one protect the chip from instant contamination. My followup would be plasma cleaning to clear off any carbon deposited between CPD and final use.
If all were done in clean room or gloved environment, then CPD - despite cost - may be the way to go both before and after processing the wafer). I would strongly recommend enthusiastic consideration.
Cheers,
Fred Monson
P.S. I'm a biologist first, so I am poking my head into an other's place. Thus, I realize the value of advice may appear depreciated by background. Howsoever, please consider the fact that since we messy organic microscopic investigators spend our lives looking at artifact and gathering together to grant each other various levels of group reality for each and every microscopic finding, we are also those who strive most eagerly for the purity of mere 3D problems. To save an ultrastructural projection on the surface of a cell, there is NOTHING like perfect CPD.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: shishkov-at-HELIX.MGH.HARVARD.EDU [mailto:shishkov-at-HELIX.MGH.HARVARD.EDU] Sent: Wednesday, January 05, 2011 3:11 PM To: Monson, Frederick
Check this product as well: http://www.vatran.com/dryice.html
-- Milen Shishkov, Ph.D. Wellman Center for Photomedicine Massachusetts General Hospital Harvard Medical School
Address: MGH BAR 712 50 Blossom St. Boston, MA 02114 Phone: (617) 726 1589 Fax: (617) 643 9208
The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail.
==============================Original Headers============================== 9, 21 -- From SHISHKOV-at-HELIX.MGH.HARVARD.EDU Wed Jan 5 14:03:24 2011 9, 21 -- Received: from phsmgmx12.partners.org (phsmgmx12.partners.org [155.52.251.71]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05K3OBC005841 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 14:03:24 -0600 9, 21 -- X-IronPort-Anti-Spam-Filtered: true 9, 21 -- X-IronPort-Anti-Spam-Result: Aq8RAEVdJE2Et4ImgWdsb2JhbACWB4YRiAMVAQEWIiS5MoYDAoMMgj4EhGiGIoMfBg 9, 21 -- Received: from phsxcon5.partners.org ([132.183.130.38]) 9, 21 -- by phsmgmx12-out.partners.org with ESMTP; 05 Jan 2011 15:03:24 -0500 9, 21 -- Received: from [132.183.44.148] ([132.183.44.148]) by PHSXCON5.partners.org with Microsoft SMTPSVC(6.0.3790.4675); 9, 21 -- Wed, 5 Jan 2011 15:03:24 -0500 9, 21 -- Message-ID: {4D24CE8B.2050900-at-helix.mgh.harvard.edu} 9, 21 -- Date: Wed, 05 Jan 2011 15:03:23 -0500 9, 21 -- From: Milen Shishkov {shishkov-at-HELIX.MGH.HARVARD.EDU} 9, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.8) Gecko/20100802 Lightning/1.0b2 Thunderbird/3.1.2 9, 21 -- MIME-Version: 1.0 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- Subject: RE: Cleaning silicon wafers 9, 21 -- X-OriginalArrivalTime: 05 Jan 2011 20:03:24.0119 (UTC) FILETIME=[9BDDF670:01CBAD13] 9, 21 -- Content-Type: text/plain; charset="iso-8859-1"; format="flowed" 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05K3OBC005841 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 29 -- From FMonson-at-wcupa.edu Wed Jan 5 16:51:39 2011 27, 29 -- Received: from WCU-EX-ET1.PASSHE.LCL (wcu-ex-et1-auth.wcupa.edu [144.26.0.82]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05Mpd4W028986 27, 29 -- for {microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 16:51:39 -0600 27, 29 -- Received: from WCU-XCH-04.PASSHE.LCL (10.32.4.191) by WCU-EX-ET1.PASSHE.LCL 27, 29 -- (10.32.0.62) with Microsoft SMTP Server (TLS) id 8.2.213.0; Wed, 5 Jan 2011 27, 29 -- 17:51:38 -0500 27, 29 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 27, 29 -- WCU-XCH-04.PASSHE.LCL ([fe80::58fd:1d03:b92e:36bd%16]) with mapi id 27, 29 -- 14.01.0255.000; Wed, 5 Jan 2011 17:51:37 -0500 27, 29 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 27, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 27, 29 -- CC: "shishkov-at-HELIX.MGH.HARVARD.EDU" {shishkov-at-HELIX.MGH.HARVARD.EDU} 27, 29 -- Subject: RE: [Microscopy] RE: Cleaning silicon wafers 27, 29 -- Thread-Topic: [Microscopy] RE: Cleaning silicon wafers 27, 29 -- Thread-Index: AQHLrRSbqF/vmwR8D0iqF4FGHovSUJPC97/g 27, 29 -- Date: Wed, 5 Jan 2011 22:51:37 +0000 27, 29 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30CAC2A-at-WCU-XCH-03.PASSHE.LCL} 27, 29 -- References: {201101052010.p05KAVoI019910-at-ns.microscopy.com} 27, 29 -- In-Reply-To: {201101052010.p05KAVoI019910-at-ns.microscopy.com} 27, 29 -- Accept-Language: en-US 27, 29 -- Content-Language: en-US 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- x-originating-ip: [10.28.57.79] 27, 29 -- Content-Type: text/plain; charset="us-ascii" 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p05Mpd4W028986 ==============================End of - Headers==============================
I'll just echo what Ken Converse and Mike Anderson have to say and add my 2 cents' worth. I work with Si wafers (patterned and unpatterned) everyday. I also have an SEM standard similar to the one you are using. I usually clean mine (after rapping the knuckles of the person who got it dirty) first with dry N2. If you have some stubborn dust particles, a short sonication in warm DI or distilled water should do it. One of those little brushes that photographers used to use to get dust off negatives would be good also, since they neutralize the static charges that make for some tenacious particles. I have deionizers all over the place, so I use those. After all the effort you put into cleaning the standard, store it in as dust-free an atmosphere and container as you have available. I store mine in the metal box it came in, in a dry nitrogen cabinet.
If some ham-handed user has stuck their finger to it, that's more difficult. CO2 snow is a wizard for removing organic contaminants such as finger oils but not everybody has access to a unit. In this case (if the stub attachment will stand it) I would try reagent-grade (or fab-grade) acetone, using it in the manner Mike suggests. I would follow this with some reagent- or fab-grade methanol as sometimes acetone can leave a residue.
On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } } I would agree with Ken on the use of a compressed gas as a first attempt at cleaning your wafer, but I would actually avoid those tetrafluoroethane dusters. It's been my experience that they can leave a film on the surface of the wafer. If you have a spare piece of polished silicon wafer hanging around you could test the duster on it to see if it leaves a film. I prefer using high purity dry nitrogen from a gas cylinder, but I have also used the compressed CO2 dusters you can find at office supply stores with good results. } } Since it looks like this wafer is etched silicon, you should be safe using a solvent if there is anything stuck on it, but I would stress that you should contact the manufacturer (Metroboost*) to make sure that they don't have some kind of a coating on it or something that would be damaged by a particular solvent. A good general choice to start would actually be deionized water, since the etch process to make the wafer probably employed that anyway. The procedure that we used during my semiconductor courses was to rinse the chip or wafer with DI water and then blow-dry it with dry nitrogen. It helps if you hold the wafer near vertical with one end resting on a lint-free paper towel and then blow the residual solvent towards the towel starting from the top of the wafer and working your way down. This generally leaves a clean, lint free, surface. } } Hope that helps! } } Mike Anderson } } *Comments made in this correspondence represent the opinion of the author and should not be construed as endorsement or recommendation by NIST. Likewise, any mention of commercial products does not imply recommendation or endorsement by NIST. } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } Sent: Monday, January 03, 2011 5:51 PM } To: Anderson, Michael } Subject: [Microscopy] RE: Cleaning silicon wafers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } I looked on Metrocal's website and didn't find anything on silicon. Is it } perhaps from Geller? Anyway, if you can find the actual manufacturer, there } should be cleaning instructions available online. } } If not, items on silicon wafers are actually pretty hardy if you don't } physically scratch them. A duster, for starters, may take care of the dust. } Is it mounted on a stub? Do you know what it's mounted with? Organic } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization } (usually Al, sometimes Au) followed by a duster. The mounting material is } more likely to present a problem than the wafer itself. } } Ken Converse } owner } } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } Sent: Monday, January 03, 2011 3:38 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Cleaning silicon wafers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings everyone, and happy new year! } } I was just working with a MetroCal (I think that's how it's spelled) } calibration wafer in an SEM, and I noticed that there is quite a bit } of dust on it. Is there a safe and effective way to clean a printed } silicon wafer which won't do any damage to it? } } --Justin A. Kraft }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 5, 24 -- From r-holdford-at-ti.com Wed Jan 5 17:49:40 2011 5, 24 -- Received: from arroyo.ext.ti.com (arroyo.ext.ti.com [192.94.94.40]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p05Nne0a013376 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Jan 2011 17:49:40 -0600 5, 24 -- Received: from dlep35.itg.ti.com ([157.170.170.118]) 5, 24 -- by arroyo.ext.ti.com (8.13.7/8.13.7) with ESMTP id p05NncwQ026078 5, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 5, 24 -- Wed, 5 Jan 2011 17:49:38 -0600 5, 24 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 5, 24 -- by dlep35.itg.ti.com (8.13.7/8.13.7) with ESMTP id p05NncTA024701; 5, 24 -- Wed, 5 Jan 2011 17:49:38 -0600 (CST) 5, 24 -- Message-ID: {4D250392.4060002-at-ti.com} 5, 24 -- Date: Wed, 05 Jan 2011 17:49:38 -0600 5, 24 -- From: Becky Holdford {r-holdford-at-ti.com} 5, 24 -- Organization: SC Packaging MPI Team 5, 24 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 5, 24 -- MIME-Version: 1.0 5, 24 -- To: Justin Kraft {kraftpiano-at-gmail.com} , 5, 24 -- MSA Listserver {Microscopy-at-microscopy.com} 5, 24 -- Subject: Re: [Microscopy] Cleaning silicon wafers 5, 24 -- References: {201101051517.p05FHXqN024496-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {201101051517.p05FHXqN024496-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
No special unit is needed for CO2 snow, just a tank of Clean! CO2. Either remove any hose, or attach the tank hose to something weighty - any block of metal with a threaded hole into which the hose can be screwed, or the like. Something to hold the hose firmly so it doesn't whip around. Turn on the tank, and the expanding CO2 makes snow. (I used to do this into a pillow case for sending samples from the Arctic.) This worked with both regular and siphon CO2 tanks. Now, I use the exhaust outlet (not "Vent") of our Polaron bomb CPD if I need snow. (Because of the weightiness of the CPD.) I suggest food grade CO2, but an absolutely dry grade may be needed for your application.
Phil
} I'll just echo what Ken Converse and Mike Anderson have to say } and add my 2 cents' worth. I work with Si wafers (patterned and } unpatterned) everyday. I also have an SEM standard similar to } the one you are using. I usually clean mine (after rapping the } knuckles of the person who got it dirty) first with dry N2. If } you have some stubborn dust particles, a short sonication in warm } DI or distilled water should do it. One of those little brushes } that photographers used to use to get dust off negatives would be } good also, since they neutralize the static charges that make for } some tenacious particles. I have deionizers all over the place, } so I use those. After all the effort you put into cleaning the } standard, store it in as dust-free an atmosphere and container as } you have available. I store mine in the metal box it came in, in } a dry nitrogen cabinet. } } If some ham-handed user has stuck their finger to it, that's more } difficult. CO2 snow is a wizard for removing organic } contaminants such as finger oils but not everybody has access to } a unit. In this case (if the stub attachment will stand it) I } would try reagent-grade (or fab-grade) acetone, using it in the } manner Mike suggests. I would follow this with some reagent- or } fab-grade methanol as sometimes acetone can leave a residue. } } On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Justin, } } } } I would agree with Ken on the use of a compressed gas as a first } } attempt at cleaning your wafer, but I would actually avoid those } } tetrafluoroethane dusters. It's been my experience that they can } } leave a film on the surface of the wafer. If you have a spare } } piece of polished silicon wafer hanging around you could test the } } duster on it to see if it leaves a film. I prefer using high } } purity dry nitrogen from a gas cylinder, but I have also used the } } compressed CO2 dusters you can find at office supply stores with } } good results. } } } } Since it looks like this wafer is etched silicon, you should be } } safe using a solvent if there is anything stuck on it, but I would } } stress that you should contact the manufacturer (Metroboost*) to } } make sure that they don't have some kind of a coating on it or } } something that would be damaged by a particular solvent. A good } } general choice to start would actually be deionized water, since } } the etch process to make the wafer probably employed that anyway. } } The procedure that we used during my semiconductor courses was to } } rinse the chip or wafer with DI water and then blow-dry it with dry } } nitrogen. It helps if you hold the wafer near vertical with one } } end resting on a lint-free paper towel and then blow the residual } } solvent towards the towel starting from the top of the wafer and } } working your way down. This generally leaves a clean, lint free, } } surface. } } } } Hope that helps! } } } } Mike Anderson } } } } *Comments made in this correspondence represent the opinion of the } } author and should not be construed as endorsement or recommendation } } by NIST. Likewise, any mention of commercial products does not } } imply recommendation or endorsement by NIST. } } } } } } -----Original Message----- } } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } } Sent: Monday, January 03, 2011 5:51 PM } } To: Anderson, Michael } } Subject: [Microscopy] RE: Cleaning silicon wafers } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Justin, } } I looked on Metrocal's website and didn't find anything on silicon. Is it } } perhaps from Geller? Anyway, if you can find the actual manufacturer, there } } should be cleaning instructions available online. } } } } If not, items on silicon wafers are actually pretty hardy if you don't } } physically scratch them. A duster, for starters, may take care of the dust. } } Is it mounted on a stub? Do you know what it's mounted with? Organic } } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization } } (usually Al, sometimes Au) followed by a duster. The mounting material is } } more likely to present a problem than the wafer itself. } } } } Ken Converse } } owner } } } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } Fax 207-647-2688 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } -----Original Message----- } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } Sent: Monday, January 03, 2011 3:38 PM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] Cleaning silicon wafers
} } Greetings everyone, and happy new year! } } } } I was just working with a MetroCal (I think that's how it's spelled) } } calibration wafer in an SEM, and I noticed that there is quite a bit } } of dust on it. Is there a safe and effective way to clean a printed } } silicon wafer which won't do any damage to it? } } } } --Justin A. Kraft } } } } -- } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Becky Holdford (r-holdford-at-ti.com) } 214-567-0360 } SC Packaging FA } Texas Instruments, Inc. } 13536 N. Central Expressway MS 940 } Dallas, TX 75243 } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 25 -- From oshel1pe-at-cmich.edu Thu Jan 6 07:09:46 2011 5, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p06D9j38028551 5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 07:09:46 -0600 5, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p06D9Zuu031961 5, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 08:09:45 -0500 5, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 5, 25 -- Thu, 6 Jan 2011 08:09:43 -0500 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {a06240801c94b6e1e6a5c-at-[141.209.160.249]} 5, 25 -- In-Reply-To: {201101052352.p05NqhEg018637-at-ns.microscopy.com} 5, 25 -- References: {201101052352.p05NqhEg018637-at-ns.microscopy.com} 5, 25 -- Date: Thu, 6 Jan 2011 08:09:39 -0500 5, 25 -- To: Microscopy-at-microscopy.com 5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 25 -- Subject: [Microscopy] Re: Cleaning silicon wafers 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-OriginalArrivalTime: 06 Jan 2011 13:09:43.0431 (UTC) FILETIME=[FBFF4970:01CBADA2] 5, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 25 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 5, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 25 -- X-CanItPRO-Stream: default 5, 25 -- X-Canit-Stats-ID: 02DQd9J1n - 93d678b9fa6a - 20110106 5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Phil: thanks bunches for this cool (pun intended) tip!
On 1/6/11 7:09 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } No special unit is needed for CO2 snow, just a tank of Clean! CO2. } Either remove any hose, or attach the tank hose to something weighty } - any block of metal with a threaded hole into which the hose can be } screwed, or the like. Something to hold the hose firmly so it doesn't } whip around. Turn on the tank, and the expanding CO2 makes snow. (I } used to do this into a pillow case for sending samples from the } Arctic.) This worked with both regular and siphon CO2 tanks. } Now, I use the exhaust outlet (not "Vent") of our Polaron bomb CPD if } I need snow. (Because of the weightiness of the CPD.) } I suggest food grade CO2, but an absolutely dry grade may be needed } for your application. } } Phil } } } I'll just echo what Ken Converse and Mike Anderson have to say } } and add my 2 cents' worth. I work with Si wafers (patterned and } } unpatterned) everyday. I also have an SEM standard similar to } } the one you are using. I usually clean mine (after rapping the } } knuckles of the person who got it dirty) first with dry N2. If } } you have some stubborn dust particles, a short sonication in warm } } DI or distilled water should do it. One of those little brushes } } that photographers used to use to get dust off negatives would be } } good also, since they neutralize the static charges that make for } } some tenacious particles. I have deionizers all over the place, } } so I use those. After all the effort you put into cleaning the } } standard, store it in as dust-free an atmosphere and container as } } you have available. I store mine in the metal box it came in, in } } a dry nitrogen cabinet. } } } } If some ham-handed user has stuck their finger to it, that's more } } difficult. CO2 snow is a wizard for removing organic } } contaminants such as finger oils but not everybody has access to } } a unit. In this case (if the stub attachment will stand it) I } } would try reagent-grade (or fab-grade) acetone, using it in the } } manner Mike suggests. I would follow this with some reagent- or } } fab-grade methanol as sometimes acetone can leave a residue. } } } } On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Justin, } } } } } } I would agree with Ken on the use of a compressed gas as a first } } } attempt at cleaning your wafer, but I would actually avoid those } } } tetrafluoroethane dusters. It's been my experience that they can } } } leave a film on the surface of the wafer. If you have a spare } } } piece of polished silicon wafer hanging around you could test the } } } duster on it to see if it leaves a film. I prefer using high } } } purity dry nitrogen from a gas cylinder, but I have also used the } } } compressed CO2 dusters you can find at office supply stores with } } } good results. } } } } } } Since it looks like this wafer is etched silicon, you should be } } } safe using a solvent if there is anything stuck on it, but I would } } } stress that you should contact the manufacturer (Metroboost*) to } } } make sure that they don't have some kind of a coating on it or } } } something that would be damaged by a particular solvent. A good } } } general choice to start would actually be deionized water, since } } } the etch process to make the wafer probably employed that anyway. } } } The procedure that we used during my semiconductor courses was to } } } rinse the chip or wafer with DI water and then blow-dry it with dry } } } nitrogen. It helps if you hold the wafer near vertical with one } } } end resting on a lint-free paper towel and then blow the residual } } } solvent towards the towel starting from the top of the wafer and } } } working your way down. This generally leaves a clean, lint free, } } } surface. } } } } } } Hope that helps! } } } } } } Mike Anderson } } } } } } *Comments made in this correspondence represent the opinion of the } } } author and should not be construed as endorsement or recommendation } } } by NIST. Likewise, any mention of commercial products does not } } } imply recommendation or endorsement by NIST. } } } } } } } } } -----Original Message----- } } } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } } } Sent: Monday, January 03, 2011 5:51 PM } } } To: Anderson, Michael } } } Subject: [Microscopy] RE: Cleaning silicon wafers } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Justin, } } } I looked on Metrocal's website and didn't find anything on silicon. Is it } } } perhaps from Geller? Anyway, if you can find the actual manufacturer, there } } } should be cleaning instructions available online. } } } } } } If not, items on silicon wafers are actually pretty hardy if you don't } } } physically scratch them. A duster, for starters, may take care of the dust. } } } Is it mounted on a stub? Do you know what it's mounted with? Organic } } } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization } } } (usually Al, sometimes Au) followed by a duster. The mounting material is } } } more likely to present a problem than the wafer itself. } } } } } } Ken Converse } } } owner } } } } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } Fax 207-647-2688 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } -----Original Message----- } } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com] } } } Sent: Monday, January 03, 2011 3:38 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: [Microscopy] Cleaning silicon wafers } } } Greetings everyone, and happy new year! } } } } } } I was just working with a MetroCal (I think that's how it's spelled) } } } calibration wafer in an SEM, and I noticed that there is quite a bit } } } of dust on it. Is there a safe and effective way to clean a printed } } } silicon wafer which won't do any damage to it? } } } } } } --Justin A. Kraft } } } } } -- } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } Becky Holdford (r-holdford-at-ti.com) } } 214-567-0360 } } SC Packaging FA } } Texas Instruments, Inc. } } 13536 N. Central Expressway MS 940 } } Dallas, TX 75243 } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 4, 23 -- From r-holdford-at-ti.com Thu Jan 6 14:10:41 2011 4, 23 -- Received: from arroyo.ext.ti.com (arroyo.ext.ti.com [192.94.94.40]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p06KAfSB026291 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 14:10:41 -0600 4, 23 -- Received: from dlep36.itg.ti.com ([157.170.170.91]) 4, 23 -- by arroyo.ext.ti.com (8.13.7/8.13.7) with ESMTP id p06KAcrD012870 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 23 -- Thu, 6 Jan 2011 14:10:38 -0600 4, 23 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 4, 23 -- by dlep36.itg.ti.com (8.13.8/8.13.8) with ESMTP id p06KAbqF025335; 4, 23 -- Thu, 6 Jan 2011 14:10:38 -0600 (CST) 4, 23 -- Message-ID: {4D2621BC.3050105-at-ti.com} 4, 23 -- Date: Thu, 06 Jan 2011 14:10:36 -0600 4, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 23 -- Organization: SC Packaging MPI Team 4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 4, 23 -- MIME-Version: 1.0 4, 23 -- To: oshel1pe-at-cmich.edu, MSA Listserver {Microscopy-at-microscopy.com} 4, 23 -- Subject: Re: [Microscopy] Re: Cleaning silicon wafers 4, 23 -- References: {201101061309.p06D9uge028766-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {201101061309.p06D9uge028766-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The EM Lab at Duke Hospital has gone digital so we have a couple of darkroom goodies to give away.
Of course, you must pay for the shipping and handling but think of all the money you'll save!
The items are:
Ilford 2150 RC print processor and 3 boxes which contain one jug of Ilford developer and one jug of Ilford fixer each. This baby is in fantastic condition and I even cleaned it so it is all sparkly and ready to move into your darkroom. You don't even have to kick the tires, plus we even have the original owners manual.
A lot of film holders for Zeiss, JEOL and maybe Hitachi. Some of these holders are for the more vintage TEM models.
If you have any questions, please e-mail Andy Kloiber at andykloiber-at-yahoo.com.
I will be on vacation next week so Andy can help you.
First come, first served and all that jazz.
More items to come as we slowly clean the darkroom out.
Enjoy your weekend,
Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#M251 Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 13, 33 -- From patpxs-at-gmail.com Thu Jan 6 16:23:45 2011 13, 33 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 13, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p06MNiSQ013108 13, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Jan 2011 16:23:44 -0600 13, 33 -- Received: by iwn40 with SMTP id 40so18633574iwn.0 13, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 06 Jan 2011 14:23:44 -0800 (PST) 13, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 33 -- d=gmail.com; s=gamma; 13, 33 -- h=domainkey-signature:mime-version:received:received:date:message-id 13, 33 -- :subject:from:to:content-type:content-transfer-encoding; 13, 33 -- bh=pGgL4fYyFzEgk2LR7a1gZ2znefAyFyPa1kOui8qX9lQ=; 13, 33 -- b=qDyRQgj/wc899NsbteFPLf4wJbAlP2doW+FxiT5BPczxmRN48fTyrwcKSpyv2KNdK2 13, 33 -- W0XL8/SxOXqg8PgDFoVhxEBZ9TZsrn5b/aU/Izej2QeczhqsXeIJRl823yyBn06xojtw 13, 33 -- 5962nh/qN+cvNuAMS2kaJ4Qs4X5KkZsE5Yu40= 13, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 33 -- d=gmail.com; s=gamma; 13, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 13, 33 -- :content-transfer-encoding; 13, 33 -- b=pC1N3kaDuT2LMo+hHbbl3vEr5LThYBX+02QLUXjDzqIsYkRQynBLiSPy1+kir+jJkP 13, 33 -- V67TxsL67s37+lThvRA/RomJoGvIf0gxK3Vo5Y8kv+vC6O7G9S1uf1mAo5m9ZdXcfdkq 13, 33 -- LQ7QpABMh94nA194wRQ1L6GVHI6Qw/kGBo/nc= 13, 33 -- MIME-Version: 1.0 13, 33 -- Received: by 10.42.171.134 with SMTP id j6mr152340icz.46.1294352624724; Thu, 13, 33 -- 06 Jan 2011 14:23:44 -0800 (PST) 13, 33 -- Received: by 10.42.218.130 with HTTP; Thu, 6 Jan 2011 14:23:44 -0800 (PST) 13, 33 -- Date: Thu, 6 Jan 2011 17:23:44 -0500 13, 33 -- Message-ID: {AANLkTinirXxELfYanarr2X9UMHEGvmJiJiUuhWOFbfBp-at-mail.gmail.com} 13, 33 -- Subject: Darkroom Equipment Free to Good Home 13, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 13, 33 -- To: Microscopy-at-microscopy.com 13, 33 -- Content-Type: text/plain; charset=ISO-8859-1 13, 33 -- Content-Transfer-Encoding: 8bit 13, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p06MNiSQ013108 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.sims-at-auckland.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] JEOL 840 anode cap position
Message: Happy New Year
It's been a while since I pulled the anode cap for cleaning, and I can neither find the part in the manual where it says to use the upper or lower position for what accelerating voltage nor remember which height I used.
Can someone remind me which position should be used for 15KV operation?
cheers Ritchie
ps I can't seem to post in the normal way and our computer guy is on holiday. Something to do with hidden attachments, I think
For our 840A with tungsten emitter: use the upper position for 6kV accelerating voltage and *less*
use the lower position for over 6kV
Arcing occurs/can occur if the upper position is used with kV} 6kV.
Phil
} Email: r.sims-at-auckland.ac.nz } Name: Ritchie Sims } } Organization: The University of Auckland } } Title-Subject: [Filtered] JEOL 840 anode cap position } } Message: Happy New Year } } It's been a while since I pulled the anode cap for cleaning, and I } can neither find the part in the manual where it says to use the } upper or lower position for what accelerating voltage nor remember } which height I used. } } Can someone remind me which position should be used for 15KV operation? } } cheers } Ritchie } } ps I can't seem to post in the normal way and our computer guy is on } holiday. Something to do with hidden attachments, I think
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 26 -- From oshel1pe-at-cmich.edu Fri Jan 7 07:04:45 2011 7, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07D4j6c001007 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 07:04:45 -0600 7, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p07D4XtK019741; 7, 26 -- Fri, 7 Jan 2011 08:04:36 -0500 7, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 26 -- Fri, 7 Jan 2011 08:02:29 -0500 7, 26 -- Mime-Version: 1.0 7, 26 -- Message-Id: {a06240800c94cbed85778-at-[141.209.160.249]} 7, 26 -- In-Reply-To: {201101070036.p070aigO004223-at-ns.microscopy.com} 7, 26 -- References: {201101070036.p070aigO004223-at-ns.microscopy.com} 7, 26 -- Date: Fri, 7 Jan 2011 08:02:26 -0500 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 26 -- Subject: Re: [Microscopy] viaWWW: JEOL 840 anode cap position 7, 26 -- Cc: r.sims-at-auckland.ac.nz 7, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 26 -- X-OriginalArrivalTime: 07 Jan 2011 13:02:29.0738 (UTC) FILETIME=[23E8BCA0:01CBAE6B] 7, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 26 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 7, 26 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 26 -- X-CanItPRO-Stream: default 7, 26 -- X-Canit-Stats-ID: 02DQB4APT - be6aa4a98493 - 20110107 7, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I do not know the exact position of the anode but the "generic" conversion for a SEM is 1mm for every 2kV anode to cathode distance!
Hope this helps?
On another note I too have had then hidden attachment rejection on several occasion recently, could there be a problem?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: r.sims-at-auckland.ac.nz [mailto:r.sims-at-auckland.ac.nz] Sent: 07 January 2011 00:34 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.sims-at-auckland.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] JEOL 840 anode cap position
Message: Happy New Year
It's been a while since I pulled the anode cap for cleaning, and I can neither find the part in the manual where it says to use the upper or lower position for what accelerating voltage nor remember which height I used.
Can someone remind me which position should be used for 15KV operation?
cheers Ritchie
ps I can't seem to post in the normal way and our computer guy is on holiday. Something to do with hidden attachments, I think
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.sims-at-auckland.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Out-of-Office auto replies
Message: Wow!
I got 21 out-of-office auto-replies to my JEOL 840 anode cap question, and no reply (yet) with information.
I guess I'll get another 21 auto-replies to this one, too.
May I quote the Listserver FAQs?
"2.) DO NOT set your Email Program to automatically reply to all messages, or to request a return receipt. If either of these are done, you run the risk of creating an Email loop within the system. This may result in a message bouncing through the system for several days, filling up everyone's mail box! "
I have found a brand new, still in the box with it's bubblewrap and packing peanuts, 11.5 inch inner diameter, 0.25 inch side wall Denton bell jar. I am giving it away, you pay the shipping.
Any takers?
Contact Andy Kloiber at andykloiber-at-yahoo.com
Have a greatr weekend!
Paula
Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#M251 Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 8, 33 -- From patpxs-at-gmail.com Fri Jan 7 11:41:51 2011 8, 33 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 8, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07Hfpuu024227 8, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 11:41:51 -0600 8, 33 -- Received: by iwn40 with SMTP id 40so19404125iwn.0 8, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 07 Jan 2011 09:41:51 -0800 (PST) 8, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 33 -- d=gmail.com; s=gamma; 8, 33 -- h=domainkey-signature:mime-version:received:received:date:message-id 8, 33 -- :subject:from:to:content-type:content-transfer-encoding; 8, 33 -- bh=AwwhUzTotrv/2vi1xwr9rYqciuQOaV8Sttk+vqgbrhM=; 8, 33 -- b=XL8Y9iZEMp70yyQmEA5NH3ShSn6EMd6/2TjjlTG94ztJLF2frs8SXyQg5ENZVlWdTo 8, 33 -- nlD3cXZNmcBctiCRCbbuLKsUdm7sjgaBY0PxnpA703VnlZNAjQ60vIQxLw1uEvTIDtxP 8, 33 -- 3T2WyQdxubQR48pJoPlX3A140Y9E9JOvaUlJA= 8, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 8, 33 -- d=gmail.com; s=gamma; 8, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 8, 33 -- :content-transfer-encoding; 8, 33 -- b=mOC6XNNp6KhRc8XzF1kTqywsEU607xsZfY7Xvw21vVTFKI9VUGBjQ0wsQJPkUb8EXR 8, 33 -- xiNNnvXoj1owX9AQVTdj7QCzfrael1xW4wNX68wrPNQmzXHwPncadazzBUobkOyW9pUc 8, 33 -- wSczRf0yP6Vd//s2m7r/OMOXZt4lp3OQkZV+w= 8, 33 -- MIME-Version: 1.0 8, 33 -- Received: by 10.42.219.198 with SMTP id hv6mr1119068icb.446.1294422110804; 8, 33 -- Fri, 07 Jan 2011 09:41:50 -0800 (PST) 8, 33 -- Received: by 10.42.219.68 with HTTP; Fri, 7 Jan 2011 09:41:50 -0800 (PST) 8, 33 -- Date: Fri, 7 Jan 2011 12:41:50 -0500 8, 33 -- Message-ID: {AANLkTimYJZ6Z_dNUDD6673Gh66=Y34odn8aMAU-my5C+-at-mail.gmail.com} 8, 33 -- Subject: Denton Bell Jar 8, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 8, 33 -- To: Microscopy-at-microscopy.com 8, 33 -- Content-Type: text/plain; charset=ISO-8859-1 8, 33 -- Content-Transfer-Encoding: 8bit 8, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p07Hfpuu024227 ==============================End of - Headers==============================
I recently read this article about Ernst Ruska and thought of sharing it with the list, many of you may have already read this but it's truly a fascinating read.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
==============================Original Headers============================== 12, 35 -- From NEERAJG-at-clemson.edu Fri Jan 7 14:16:30 2011 12, 35 -- Received: from mailclient1.clemson.edu (mailclient1.clemson.edu [130.127.237.180]) 12, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07KGUVo012098 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 14:16:30 -0600 12, 35 -- Received: from mailclient1.clemson.edu (localhost.clemson.edu [127.0.0.1]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGTlS026918 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGSJc026913 12, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 12, 35 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Fri, 12, 35 -- 7 Jan 2011 15:16:28 -0500 12, 35 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 12, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 35 -- Date: Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Subject: Re: Ernst Ruska 12, 35 -- Thread-Topic: Re: Ernst Ruska 12, 35 -- Thread-Index: Acuup8SIOJpgV3B5RIecFUESkna64A== 12, 35 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F99385626E6-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 12, 35 -- Accept-Language: en-US 12, 35 -- Content-Language: en-US 12, 35 -- X-MS-Has-Attach: 12, 35 -- X-MS-TNEF-Correlator: 12, 35 -- acceptlanguage: en-US 12, 35 -- Content-Type: text/plain; charset="iso-8859-1" 12, 35 -- MIME-Version: 1.0 12, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 12, 35 -- definitions=2011-01-07_10:2011-01-07,2011-01-07,1970-01-01 signatures=0 12, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 12, 35 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 12, 35 -- engine=5.0.0-1012030000 definitions=main-1101070075 12, 35 -- Content-Transfer-Encoding: 8bit 12, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p07KGUVo012098 ==============================End of - Headers==============================
Two positions are available in electron microscopy at the Department of Materials, University of Oxford, UK:
Departmental Lecturer in Aberration Corrected Electron Microscopy Grade 8 / Salary £36,715 to £43,840 pa / Post Ref: DJ10/29
Senior Support Scientist in Electron Microscopy Grade 8 / Salary £36,715 to £43,840 pa / Post Ref: DJ10/30
See http://www.materials.ox.ac.uk/vacancies.html for further details.
Dr Peter Nellist {peter.nellist-at-materials.ox.ac.uk} University Lecturer in Materials Tutorial Fellow at Corpus Christi College Department of Materials University of Oxford Parks Road OXFORD OX1 3PH UK
Phone: +44 (0)1865-273656 Fax: +44 (0)1865-283329
Sent on behalf of Pete Nellist.
Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk
==============================Original Headers============================== 2, 28 -- From ron.doole-at-materials.ox.ac.uk Mon Jan 10 07:00:06 2011 2, 28 -- Received: from relay4.mail.ox.ac.uk (relay4.mail.ox.ac.uk [129.67.1.163]) 2, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0AD06Tm001381 2, 28 -- for {Microscopy-at-Microscopy.Com} ; Mon, 10 Jan 2011 07:00:06 -0600 2, 28 -- Received: from smtp3.nexus.ox.ac.uk ([163.1.154.137] helo=exht03.ad.oak.ox.ac.uk) 2, 28 -- by relay4.mail.ox.ac.uk with esmtp (Exim 4.71) 2, 28 -- (envelope-from {ron.doole-at-materials.ox.ac.uk} ) 2, 28 -- id 1PcHLy-0005Iq-Dy 2, 28 -- for Microscopy-at-Microscopy.Com; Mon, 10 Jan 2011 13:00:06 +0000 2, 28 -- Received: from EXMBX02.ad.oak.ox.ac.uk ([169.254.2.237]) by 2, 28 -- exht03.ad.oak.ox.ac.uk ([163.1.154.54]) with mapi; Mon, 10 Jan 2011 13:00:06 2, 28 -- +0000 2, 28 -- From: Ron Doole {ron.doole-at-materials.ox.ac.uk} 2, 28 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 2, 28 -- Date: Mon, 10 Jan 2011 13:00:05 +0000 2, 28 -- Subject: 2 TEM positions available at Oxford - UK 2, 28 -- Thread-Topic: 2 TEM positions available at Oxford - UK 2, 28 -- Thread-Index: AQHLsMZNsbXUBfxSZkGcITIyiqX9Xw== 2, 28 -- Message-ID: {FB563F1F5A8DDB49A34CB648BD23ACF932D1E38844-at-EXMBX02.ad.oak.ox.ac.uk} 2, 28 -- Accept-Language: en-US, en-GB 2, 28 -- Content-Language: en-GB 2, 28 -- X-MS-Has-Attach: 2, 28 -- X-MS-TNEF-Correlator: 2, 28 -- acceptlanguage: en-US, en-GB 2, 28 -- Content-Type: text/plain; charset="iso-8859-1" 2, 28 -- MIME-Version: 1.0 2, 28 -- Content-Transfer-Encoding: 8bit 2, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0AD06Tm001381 ==============================End of - Headers==============================
} Date: Mon, 10 Jan 2011 16:08:32 -0800 (PST) } From: Richard Kurtz {rkurtz-at-commack.k12.ny.us} } To: oshel1pe-at-cmich.edu } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 10, } 2011 at 04:08:30 PM. } } realname - Richard Kurtz } Email - rkurtz-at-commack.k12.ny.us } ORGANIZATION - Commack High School } EDUCATION - 9-12th Grade High School } LOCATION - Commack, NY, USA } SUBJECT_OF_QUESTION - image of a insect foot pad } QUESTION - To Whom it may concern, } } I am a high school science teacher at Commack High School on Long Island, NY } I have a pair of students investigating the walking ability of the } Indian walking stick. The have the ability to walk on a host of } materials upside down and vertically. } } They and I wanted to find a place that would be able to look at } their footpads microscopically. } We are very interested in their surface, what structures are on } their feet. We want to know } were we may be able to find a place that would be able and willing } to help us with this. } } I have no idea how this could happen but just asking. } } Any advice or suggestions that you could provide would be great } } Thanks so much } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 23 -- From oshel1pe-at-cmich.edu Tue Jan 11 10:56:39 2011 1, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0BGud3d028995 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 10:56:39 -0600 1, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0BGucD6001278 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 11:56:39 -0500 1, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 23 -- Tue, 11 Jan 2011 11:56:38 -0500 1, 23 -- Mime-Version: 1.0 1, 23 -- Message-Id: {a06240804c9523bd6d418-at-[141.209.160.249]} 1, 23 -- Date: Tue, 11 Jan 2011 11:56:34 -0500 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 23 -- Subject: Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 1, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 23 -- X-OriginalArrivalTime: 11 Jan 2011 16:56:38.0766 (UTC) FILETIME=[836F48E0:01CBB1B0] 1, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 23 -- X-CanItPRO-Stream: default 1, 23 -- X-Canit-Stats-ID: 02DSgUCAP - d6b3e6a7655b - 20110111 1, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This is something we could do as part of a Bugscope session, and I invite you to apply.
Thank you
Scott, with Bugscope
-- Microscopy Suite | Imaging Technology Group | Beckman Institute for Advanced Science and Technology | University of Illinois at Urbana-Champaign | 405 North Mathews Avenue, Urbana IL 61801 | 217 265 5071 | 217 244 6219 (fax) sjrobin-at-illinois.edu | http://itg.beckman.illinois.edu | http://bugscope.beckman.illinois.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Tuesday, January 11, 2011 11:09 AM To: Robinson, Scott J.
} Date: Mon, 10 Jan 2011 16:08:32 -0800 (PST) } From: Richard Kurtz {rkurtz-at-commack.k12.ny.us} } To: oshel1pe-at-cmich.edu } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Monday, January 10, } 2011 at 04:08:30 PM. } } realname - Richard Kurtz } Email - } ORGANIZATION - Commack High School } EDUCATION - 9-12th Grade High School } LOCATION - Commack, NY, USA } SUBJECT_OF_QUESTION - image of a insect foot pad QUESTION - To Whom it } may concern, } } I am a high school science teacher at Commack High School on Long } Island, NY I have a pair of students investigating the walking ability } of the Indian walking stick. The have the ability to walk on a host of
} materials upside down and vertically. } } They and I wanted to find a place that would be able to look at their } footpads microscopically. } We are very interested in their surface, what structures are on their } feet. We want to know were we may be able to find a place that would } be able and willing to help us with this. } } I have no idea how this could happen but just asking. } } Any advice or suggestions that you could provide would be great } } Thanks so much } -- ************************************************************************ *************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ************************************************************************ ****************
==============================Original Headers============================== 1, 23 -- From oshel1pe-at-cmich.edu Tue Jan 11 10:56:39 2011 1, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0BGud3d028995 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 10:56:39 -0600 1, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0BGucD6001278 1, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 11:56:39 -0500 1, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 23 -- Tue, 11 Jan 2011 11:56:38 -0500 1, 23 -- Mime-Version: 1.0 1, 23 -- Message-Id: {a06240804c9523bd6d418-at-[141.209.160.249]} 1, 23 -- Date: Tue, 11 Jan 2011 11:56:34 -0500 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 23 -- Subject: Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 1, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 23 -- X-OriginalArrivalTime: 11 Jan 2011 16:56:38.0766 (UTC) FILETIME=[836F48E0:01CBB1B0] 1, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 23 -- X-CanItPRO-Stream: default 1, 23 -- X-Canit-Stats-ID: 02DSgUCAP - d6b3e6a7655b - 20110111 1, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 23 -- From sjrobin-at-illinois.edu Tue Jan 11 11:15:35 2011 16, 23 -- Received: from BI-ADMEX.beckman.uiuc.edu (bi-admcls4.beckman.uiuc.edu [130.126.126.13]) 16, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0BHFZDD012798 16, 23 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 11:15:35 -0600 16, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 23 -- Content-class: urn:content-classes:message 16, 23 -- MIME-Version: 1.0 16, 23 -- Content-Type: text/plain; 16, 23 -- charset="us-ascii" 16, 23 -- Subject: RE: [Microscopy] Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 16, 23 -- Date: Tue, 11 Jan 2011 11:13:16 -0600 16, 23 -- Message-ID: {87D63F6D93DB14418272BF5859FFF2C9139BA093-at-BI-ADMEX.beckman-admin.uiuc.edu} 16, 23 -- In-Reply-To: {201101111708.p0BH8ws9011457-at-ns.microscopy.com} 16, 23 -- X-MS-Has-Attach: 16, 23 -- X-MS-TNEF-Correlator: 16, 23 -- Thread-Topic: [Microscopy] Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY 16, 23 -- Thread-Index: AcuxskB6rr2OY6E+S/atT0dJ/Z4wLQAAEgVQ 16, 23 -- References: {201101111708.p0BH8ws9011457-at-ns.microscopy.com} 16, 23 -- From: "Robinson, Scott J." {sjrobin-at-illinois.edu} 16, 23 -- To: {oshel1pe-at-cmich.edu} , {microscopy-at-microscopy.com} 16, 23 -- Cc: {rkurtz-at-commack.k12.ny.us} , "bugscope" {bugscope-at-beckman.illinois.edu} 16, 23 -- Content-Transfer-Encoding: 8bit 16, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0BHFZDD012798 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lgiannuzzi-at-comcast.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] FIB/SEM Specialist: open position at Weatherford
Message: FIB-SEM Specialist Weatherford Laboratories Houston, TX Description: Weatherford Laboratories is seeking qualified candidates to fill the position of FIB-SEM Specialist at its Houston, Texas facility. The FIB-SEM Specialist is responsible for operating and maintaining a dual-column Focused Ion Beam (FIB) microscope / Scanning Electron Microscope (SEM) analytical instrument primarily to provide characterization of rock material from unconventional reservoirs. The FIB microscopy work includes in-situ high resolution imaging, micro-chemical analysis, and site-specific 3-D feature analysis, utilizing energy dispersive spectrometry (EDS) / secondary and backscatter electron characterization techniques. The FIB Microscopist will utilize their skills with dual-column FIB /electron microscopy to evaluate rock properties; perform data /image processing, including 3-D reconstruction and visualization of FIB imaging/analytical data; analyze and interpret comprehensive and multiple datasets; and communicate results to clients by oral and written reports. Qualifications: A bachelorís degree in a relevant scientific area is required, advanced degree preferred. Experience with operation of a dual-column FIB-SEM microscope is required. Image processing and geologic background a strong plus. Salary/Benefits: Commensurate with degree and experience. Company provides competitive benefit packages. Salary Range: Commensurate with degree and experience. Contact Information: Name: LouAnn Reid Company: Weatherford Laboratories Address: 5200 N. Sam Houston Pkwy W., Suite 500, Houston, TX 77086 Email: louann.reid-at-weatherfordlabs.com Website: www.weatherford.com to apply for position Open Search: 12/9/10 Close Search: Until filled
We are in the process of revising our recharge rates for our core microscopy facility. Although we do not solicit imaging projects from external customers, we do get some...usually from former students or companies associated with university faculty.
Our major instrumentation was purchased with the help of federal grants. The granting agencies do not have a problem with our using them for external projects provided that all internal clients funded by that agency, especially those whose projects were used for justification, have as much access as desired. However, they stipulate that any charges be equivalent to those of commercial providers.
Thus I need to talk to for-profit companies, who provide SEM and TEM, regarding current rates so that we can comply with this mandate. I would greatly appreciate your contacting me if you will be willing to help me in this task.
Thanks, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 10, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:12:30 2011 10, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2CTKo025083 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:12:29 -0600 10, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 10, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2CTgD028619 10, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:12:29 -0500 10, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB01F.purdue.lcl 10, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:12:28 -0500 10, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 28 -- Date: Tue, 11 Jan 2011 21:12:27 -0500 10, 28 -- Subject: Commercial microscopy rates 10, 28 -- Thread-Topic: Commercial microscopy rates 10, 28 -- Thread-Index: Acux/iiHU9Xf5zZHtEiQOde8ZXwsEA== 10, 28 -- Message-ID: {C952783B.AFE1%dsherman-at-purdue.edu} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- X-PMX-Version: 5.5.9.388399 10, 28 -- X-PerlMx-Virus-Scanned: Yes 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2CTKo025083 ==============================End of - Headers==============================
Hi all, We have someone who wants images of somatic cells (mainly leukocytes) in milk using light microscopy. I am looking for a staining method and would appreciate any hints to accomplish this. This is a bit out of my area with my being primarily an electron microscopist.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:15:54 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2Fs9b032153 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:15:54 -0600 6, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2FsDk029105 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:15:54 -0500 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB02F.purdue.lcl 6, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:15:53 -0500 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500 6, 28 -- Subject: Staining somatic cells in milk 6, 28 -- Thread-Topic: Staining somatic cells in milk 6, 28 -- Thread-Index: Acux/qIfNhwwnZONaES65KSOZh3WKg== 6, 28 -- Message-ID: {C9527907.AFE6%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2Fs9b032153 ==============================End of - Headers==============================
The main question is: can you isolate the cells, stain them and put them back in the milk?
If not, here are 2 possibilities:
The easiest one I can see is to incubate the sample with a DNA dye such as DAPI or Hoechst. These dyes are not expensive and are used by all microscopists doing fluorescence. Just mix the milk with a little bit of dye and observe...that's all! The only drawback is that you need a fluorescence microscope, but nowadays they are everywhere! I hope that the milk doesn't have an intrinsic autofluorescence in the UV range!
Alternatively tetrazolium salts can be metabolized by live cells into formazan, which are dyes. Some formazan dyes are soluble, others are not. Just choose an insoluble, like MTT. In this case you will only stain live cells, not the dead one. Tetrazolium salts are not expensive.
You won't be able to make the difference with germinal cells, though :-D I would avoid to use dyes which stain the membranes, since they may concentrate in the lipid droplets of the milk.
Regards, Stephane
----- Original Message ---- X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} To: nizets2-at-yahoo.com Sent: Wed, January 12, 2011 3:18:44 AM
Hi all, We have someone who wants images of somatic cells (mainly leukocytes) in milk using light microscopy. I am looking for a staining method and would appreciate any hints to accomplish this. This is a bit out of my area with my being primarily an electron microscopist.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:15:54 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2Fs9b032153 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:15:54 -0600 6, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2FsDk029105 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:15:54 -0500 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB02F.purdue.lcl 6, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:15:53 -0500 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500 6, 28 -- Subject: Staining somatic cells in milk 6, 28 -- Thread-Topic: Staining somatic cells in milk 6, 28 -- Thread-Index: Acux/qIfNhwwnZONaES65KSOZh3WKg== 6, 28 -- Message-ID: {C9527907.AFE6%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2Fs9b032153 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From nizets2-at-yahoo.com Wed Jan 12 05:50:11 2011 25, 37 -- Received: from nm26-vm0.bullet.mail.sp2.yahoo.com (nm26-vm0.bullet.mail.sp2.yahoo.com [98.139.91.230]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0CBoBCs008689 25, 37 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 05:50:11 -0600 25, 37 -- Received: from [98.139.91.66] by nm26.bullet.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- Received: from [98.139.91.52] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- Received: from [127.0.0.1] by omp1052.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- X-Yahoo-Newman-Property: ymail-3 25, 37 -- X-Yahoo-Newman-Id: 890075.12821.bm-at-omp1052.mail.sp2.yahoo.com 25, 37 -- Received: (qmail 70090 invoked by uid 60001); 12 Jan 2011 11:50:10 -0000 25, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1294833010; bh=dKrxBNFW4ytXLYCP+nF97/lTpfbjIbWO/CNk0eS6YLI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=q2x0SSV6hKeozJ/R2FDsG3Be7Bc1cL/sVY/Q2jF+WrWZyl1mT/+Es349sXBn5Ej5NBlXIoGkwQO9jy1f/8w2K9SEpLT2O8kiRweASJ9QpAWZyii7gF13zKLgsw7Vg9dHMMMLHTuY2YSvDVkLX/inAA8QzvQaT9WNEWFVScRBz0A= 25, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 25, 37 -- s=s1024; d=yahoo.com; 25, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 25, 37 -- b=WTSHqBV9WqG6TdCWmU2Ukyvco5EtgGayejwD2oBbwtIAJwvw1Ur9SJO4JU1QMOVf7oTnvdANnGd9KopFMxzn6/0XLBmqU6ympJbPZrW8jc9A61gtLqro6+/0GY/gUEbfMchZ6lCQzUsdsoLtSnLfwK+/X6i/e51LwGTD3E6oOnA=; 25, 37 -- Message-ID: {218554.51777.qm-at-web110816.mail.gq1.yahoo.com} 25, 37 -- X-YMail-OSG: cMbsF_AVM1mSgmmzGAxvCz_JzlmJMeO2iKQLzx_I.kMfHoO 25, 37 -- MxtJ0BHEctYiA1dkyctHUsh.4mslgFpgRH5hDVrjWVC51aJidnclUQXItlgk 25, 37 -- mRWuBEErd5.7JQSZ8RjqYbiC5rFmEY7kXxJCEBQQ_LorYUopqnKzbfcLgjqv 25, 37 -- sTmqSi7Sveo6Rk7ixzVAGjjUz37IAjC3c6jihVk33zcUWc6VMd_pAi9hbQcW 25, 37 -- q3dkOAmBM9iC7VwgUud5W9S.Mwc_3P4vdz9R1dZeVOV4k8Q7SxAzZ01myUTG 25, 37 -- EMkG7XqdZgnQFvtXCZEyeYjD443V0tac8.8BF4qZpFFdoKvbHmqXQzfMr_7s 25, 37 -- UDR_jLSbGKPZ6N3HgtEoPauU1G2My2DfhxJEhye4VpNfY14m2CjXHlYkL2IV 25, 37 -- 6orYLno7cZQFrLWo- 25, 37 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Wed, 12 Jan 2011 03:50:09 PST 25, 37 -- X-Mailer: YahooMailRC/553 YahooMailWebService/0.8.107.285259 25, 37 -- References: {201101120218.p0C2Ii52009748-at-ns.microscopy.com} 25, 37 -- Date: Wed, 12 Jan 2011 03:50:09 -0800 (PST) 25, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 25, 37 -- Subject: Re: [Microscopy] Staining somatic cells in milk 25, 37 -- To: dsherman-at-purdue.edu 25, 37 -- Cc: microscopy-at-microscopy.com 25, 37 -- In-Reply-To: {201101120218.p0C2Ii52009748-at-ns.microscopy.com} 25, 37 -- MIME-Version: 1.0 25, 37 -- Content-Type: text/plain; charset=iso-8859-1 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CBoBCs008689 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mseabul-at-aerotek.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job Opening: Applications Scientist TEM/STEM of catalysts
Message: Hi! My name is Melanie and I work for Aerotek Scientific, a technical staffing firm in the Chicagoland area. I currently have an opening for a microscopy position in the area. Some details on this position are as follows:
-individual must be familiar with working with an STEM/TEM electron microscope, in support of the materials and catalyst development of the company -Individual will collaborate with and assist senior electron microscopists in the development of new capabilities for a new probe aberration corrected STEM/TEM microscope -Will be designing and conducting experiments and techniques using this microscope -operating the aberration corrected microscope for characterization of nanomaterials and catalysts -develope advanced applications for STEM, EELS, EDS tomography, and in-situ for beam sensitive nanomaterials
Best candidate will have Ph.D. in Materials Science, chemistry, physics or metallurgy with specialization in electron microscopy, experience with at least one of EDS, EELS, tomography or in-situ techniques and 0-5 years in a related field. Strong understanding of instrumentation and other characterization techniques such as X-ray diffraction are a plus.
Please let me know if you or anyone that you know may be interested and qualified for this position, I am actively seeking great candidates! Thank you!
Melanie Seabul Aerotek Scientific 1790 Nations Drive, Suite 116 Gurnee, IL 60031
Direct Line: 847-782-5447 E-mail: mseabul-at-aerotek.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mseabul-at-aerotek.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hi! My name is Melanie and I work for Aerotek Scientific, a technical staffing firm in the Chicagoland area. I currently have a job opportunity for a Metallurgy/Corrosion scientist. Some details on this position are as follows:
-individual must have 10 years of on the job experience as a metallurgist and corrosion scientist -strong analytical chemistry skills -must have strong understanding of the degradation of metal at its surface through interaction with chemicals -working on project basis, researching and studying structures and chem. Properties of metals -experience working/running/analyzing the following tests: materials analysis, failure investigation, materials conservation, organic chemistry, analytical chemistry, petrographic evaluation -will be involved in running these tests: nondestructive evaluation, water penetration testing, strain and fracture monitoring, vibration monitoring, load testing, blast monitoring, frequency identification
The best candidate will have a masters degree, or higher, in a related scientific field with 10 years of experience. Please let me know if you or anyone that you know may be interested and qualified for this position as I am actively seeking great candidates! Thank you!
Melanie Seabul Aerotek Scientific 1790 Nations Drive, Suite 116 Gurnee, IL 60031
Direct Line: 847-782-5447 E-mail: mseabul-at-aerotek.com
As the article describes, the early version of the instrument could only reach modest resolutions and we have to remember that the thermal advancement Ultramicrotome by Porter and Bloom was reported in 1953. We know now what the combination of TEM and ultramicrotome did for biology and other fields, and gradually many crucial discoveries were made using the TEM which eventually could attain atomic resolutions. This is somewhat similar to discovery of LASER, many of the key people who worked on the LASER during its conception were awarded the Nobel prize later (Luckily the TEM didn't go through the lawsuits and patent battles that the LASER did). This may be true of our times too, we have yet to fully grasp the potential of recent advances in super-resolution optical imaging.
Best,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
-----Original Message----- X-from: Carol Heckman [mailto:heckman-at-bgsu.edu] Sent: Sunday, January 09, 2011 12:00 PM To: Neeraj Gohad
Happy New Year Everyone!
I recently read this article about Ernst Ruska and thought of sharing it with the list, many of you may have already read this but it's truly a fascinating read.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
==============================Original Headers============================== 12, 35 -- From NEERAJG-at-clemson.edu Fri Jan 7 14:16:30 2011 12, 35 -- Received: from mailclient1.clemson.edu (mailclient1.clemson.edu [130.127.237.180]) 12, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p07KGUVo012098 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 14:16:30 -0600 12, 35 -- Received: from mailclient1.clemson.edu (localhost.clemson.edu [127.0.0.1]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGTlS026918 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 12, 35 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p07KGSJc026913 12, 35 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 12, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 12, 35 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Fri, 12, 35 -- 7 Jan 2011 15:16:28 -0500 12, 35 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 12, 35 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 35 -- Date: Fri, 7 Jan 2011 15:16:29 -0500 12, 35 -- Subject: Re: Ernst Ruska 12, 35 -- Thread-Topic: Re: Ernst Ruska 12, 35 -- Thread-Index: Acuup8SIOJpgV3B5RIecFUESkna64A== 12, 35 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F99385626E6-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 12, 35 -- Accept-Language: en-US 12, 35 -- Content-Language: en-US 12, 35 -- X-MS-Has-Attach: 12, 35 -- X-MS-TNEF-Correlator: 12, 35 -- acceptlanguage: en-US 12, 35 -- Content-Type: text/plain; charset="iso-8859-1" 12, 35 -- MIME-Version: 1.0 12, 35 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 12, 35 -- definitions=2011-01-07_10:2011-01-07,2011-01-07,1970-01-01 signatures=0 12, 35 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 12, 35 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 12, 35 -- engine=5.0.0-1012030000 definitions=main-1101070075 12, 35 -- Content-Transfer-Encoding: 8bit 12, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p07KGUVo012098 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 38 -- From NEERAJG-at-clemson.edu Wed Jan 12 13:01:16 2011 28, 38 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 28, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJ1F57006586 28, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:01:15 -0600 28, 38 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 28, 38 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJ1FoX021328 28, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:01:15 -0500 28, 38 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 28, 38 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJ1Ebd021322 28, 38 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 28, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:01:14 -0500 28, 38 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 28, 38 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 28, 38 -- 12 Jan 2011 14:01:14 -0500 28, 38 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 28, 38 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 28, 38 -- Date: Wed, 12 Jan 2011 14:01:12 -0500 28, 38 -- Subject: RE: [Microscopy] Re: Ernst Ruska 28, 38 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 28, 38 -- Thread-Index: AcuuqRxxdQXGzIelSOiA0dPGxl0gdwBdYirHAJlPKZA= 28, 38 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562BAC-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 28, 38 -- References: {201101072026.p07KQ4qq026821-at-ns.microscopy.com} 28, 38 -- {E76D862B01CC7845BF120D9591635FA0156D50-at-MAIL4.bgsu.edu} 28, 38 -- In-Reply-To: {E76D862B01CC7845BF120D9591635FA0156D50-at-MAIL4.bgsu.edu} 28, 38 -- Accept-Language: en-US 28, 38 -- Content-Language: en-US 28, 38 -- X-MS-Has-Attach: 28, 38 -- X-MS-TNEF-Correlator: 28, 38 -- acceptlanguage: en-US 28, 38 -- Content-Type: text/plain; charset="us-ascii" 28, 38 -- MIME-Version: 1.0 28, 38 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 28, 38 -- definitions=2011-01-12_05:2011-01-12,2011-01-12,1970-01-01 signatures=0 28, 38 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 28, 38 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 28, 38 -- engine=5.0.0-1012030000 definitions=main-1101120104 28, 38 -- Content-Transfer-Encoding: 8bit 28, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CJ1F57006586 ==============================End of - Headers==============================
Sorry, Neera - the Porter-Blum microtome was mechanical advance. Simple, reliable, unbreakable.
Caroline
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 3, 18 -- From schooley-at-mcn.org Wed Jan 12 13:22:17 2011 3, 18 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJMGbL022762 3, 18 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:22:17 -0600 3, 18 -- Received: from [66.81.41.72] 3, 18 -- by smtp1.mcn.org with esmtpa (Exim 4.63) 3, 18 -- (envelope-from {schooley-at-mcn.org} ) 3, 18 -- id 1Pd6Gq-0007FY-56; Wed, 12 Jan 2011 11:22:13 -0800 3, 18 -- Mime-Version: 1.0 3, 18 -- Message-Id: {a06200706c953adc1f12a-at-[66.81.41.72]} 3, 18 -- In-Reply-To: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- References: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- Date: Wed, 12 Jan 2011 11:21:19 -0800 3, 18 -- To: NEERAJG-at-clemson.edu 3, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 18 -- Subject: Re: [Microscopy] Ernst Ruska 3, 18 -- Cc: {microscopy-at-microscopy.com} 3, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
To All Microscopists Researching High Resolution Biological Structure:
I call your attention to a new Special Issue of the Elsevier journal, MICRON, which has just been published (Volume 42, number 2: February, 2011). This originated from a realization that several different types of modern microscopes now can readily achieve atomic and sub-Ã…ngstrom levels of resolution with many inorganic specimens; since the resolution with biological specimens still only rarely obtains better than 3Ã… (0.3nm), this deficient resolution must not be caused by the instrument, but rather is due to inadequacies in specimen preparation and in structural preservation during data collection. These two important subjects are what the Special Issue deals with, in the context of a wide variety of biospecimens and use of several different kinds of microscopes (TEM, STEM, SEM, and AFM).
The 11 new review articles within this Special Issue concern the many practical problems for trying to produce better specimen preparation and preservation. Each is written by expert practicing microscopists. Many examples of research results and technical advances are illustrated by selected micrographs, diagrams, and charts, and are documented by extensive literature citations. Practical interest is expected from research students, technicians, and postdocs, as well as from experienced microscopists. We hope that these new reviews will be both informative and stimulating for all biological microscopists trying to obtain structural data with even higher resolution levels than are currently possible.
A complete table of contents follows this message. This Special Issue is included with all regular subscriptions to MICRON, and now is available to those at many institutions via the Science Direct website (http://www.sciencedirect.com).
Sincerely, and Happy New Year (2011),
BILL MASSOVER
William H. Massover Guest Editor, MICRON wmassover-at-anl.gov
Special Issue
MICRON Volume 42(2) February 2011: Contents
"Biological Specimen Preparation and Preservation for High Resolution Microscopies"
97 Introduction to Special Issue of Micron: “Biological specimen preparation and preservation for high resolution microscopiesâ€
W.H. Massover
100 Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage
K. Mitsuoka
107 Electron cryomicroscopy of membrane proteins: Specimen preparation for two-dimensional crystals and single particles
I. Schmidt-Krey and J.L. Rubinstein
117 Negative staining and cryo-negative staining of macromolecules and viruses for TEM
S. De Carlo and J. Robin Harris
132 Specimen preparation for electron diffraction of thin crystals
H. Wang and K.H. Downing
141 New and unconventional approaches for advancing resolution in biological transmission electron microscopy by improving macromolecular specimen preparation and preservation
W.H. Massover
152 Cryo-electron tomography on vitrified sections: A critical analysis of benefits and limitations for structural cell biology
C. Bouchet-Marquis and A. Hoenger
163 Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and nanodevices
R.D. Powell and J.F. Hainfeld
175 Low voltage high-resolution SEM (LVHRSEM) for biological structural and molecular analysis
H. Schatten
186 Assessing the structure and function of single biomolecules with scanning transmission electron and atomic force microscopes
S.A. Müller, D.J. Müller and A. Engel
196 Preparation of DNA and nucleoprotein samples for AFM imaging
Y.L. Lyubchenko
==============================Original Headers============================== 41, 33 -- From wmassover-at-anl.gov Wed Jan 12 13:24:24 2011 41, 33 -- Received: from mailhost.anl.gov (mailhost.anl.gov [130.202.113.50]) 41, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJOO1n026491 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 41, 33 -- Received: from mailhost.anl.gov (mailhost.anl.gov [130.202.113.50]) 41, 33 -- by localhost.anl.gov (Postfix) with ESMTP id 483D854 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- Received: from zimbra.anl.gov (zimbra.anl.gov [130.202.101.12]) 41, 33 -- by mailhost.anl.gov (Postfix) with ESMTP id 2B5AD53 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 41, 33 -- by zimbra.anl.gov (Postfix) with ESMTP id 1887212437 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- X-Virus-Scanned: amavisd-new at zimbra.anl.gov 41, 33 -- Received: from zimbra.anl.gov ([127.0.0.1]) 41, 33 -- by localhost (zimbra.anl.gov [127.0.0.1]) (amavisd-new, port 10024) 41, 33 -- with ESMTP id vaJ92nLBKz-2 for {Microscopy-at-Microscopy.Com} ; 41, 33 -- Wed, 12 Jan 2011 13:24:24 -0600 (CST) 41, 33 -- Received: from zimbra.anl.gov (zimbra.anl.gov [130.202.101.12]) 41, 33 -- by zimbra.anl.gov (Postfix) with ESMTP id F330712436 41, 33 -- for {Microscopy-at-Microscopy.Com} ; Wed, 12 Jan 2011 13:24:23 -0600 (CST) 41, 33 -- Date: Wed, 12 Jan 2011 13:24:23 -0600 (CST) 41, 33 -- From: William Massover {wmassover-at-anl.gov} 41, 33 -- To: Microscopy-at-Microscopy.Com 41, 33 -- Message-ID: {2122403145.55417.1294860263970.JavaMail.root-at-zimbra.anl.gov} 41, 33 -- In-Reply-To: {1740785534.55378.1294859911603.JavaMail.root-at-zimbra.anl.gov} 41, 33 -- Subject: A New Resource for Trying to Improve Resolution with Biospecimens 41, 33 -- in TEM, STEM, SEM, and AFM 41, 33 -- MIME-Version: 1.0 41, 33 -- Content-Type: text/plain; charset=utf-8 41, 33 -- X-Originating-IP: [130.202.101.12] 41, 33 -- Content-Transfer-Encoding: 8bit 41, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CJOO1n026491 ==============================End of - Headers==============================
I did a few years looking at milk in-situ in mammary gland including lactating and involuting where we saw lots of somatic cells.
Hoechst or DAPI will work fine, there is not a significant problem with autofluorescence even in the UV range, and you can differentiate the types of cells a little by the staining pattern.
If you do not want to go the fluorescence root you could use plain old heamotoxylin and eosin. This has an added advantage, leucocytes are highly eosinophillic and are characterised very well with standard H&E. You will also get good ID on other cell types.
If you do not want spin down your cells you could try a standard blood smear prep on a coated slide and fix them on with formalin or paraformaldehyde. Alternatively you could add low melting point agar or agarose and set them as a gel. This will allow processing through paraffin if you need (although you lose your water and milk lipids of course.
Thanks
Ray
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, 13 January 2011 1:00 a.m. To: Ray Gilbert
Hi Debby!
The main question is: can you isolate the cells, stain them and put them back in the milk?
If not, here are 2 possibilities:
The easiest one I can see is to incubate the sample with a DNA dye such as DAPI or Hoechst. These dyes are not expensive and are used by all microscopists doing fluorescence. Just mix the milk with a little bit of dye and observe...that's all! The only drawback is that you need a fluorescence microscope, but nowadays they are everywhere! I hope that the milk doesn't have an intrinsic autofluorescence in the UV range!
Alternatively tetrazolium salts can be metabolized by live cells into formazan, which are dyes. Some formazan dyes are soluble, others are not. Just choose an insoluble, like MTT. In this case you will only stain live cells, not the dead one. Tetrazolium salts are not expensive.
You won't be able to make the difference with germinal cells, though :-D I would avoid to use dyes which stain the membranes, since they may concentrate in the lipid droplets of the milk.
Regards, Stephane
----- Original Message ---- X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} To: nizets2-at-yahoo.com Sent: Wed, January 12, 2011 3:18:44 AM
Hi all, We have someone who wants images of somatic cells (mainly leukocytes) in milk using light microscopy. I am looking for a staining method and would appreciate any hints to accomplish this. This is a bit out of my area with my being primarily an electron microscopist.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Tue Jan 11 20:15:54 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0C2Fs9b032153 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 20:15:54 -0600 6, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0C2FsDk029105 6, 28 -- for {microscopy-at-microscopy.com} ; Tue, 11 Jan 2011 21:15:54 -0500 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.164]) by WPPEXHUB02F.purdue.lcl 6, 28 -- ([::1]) with mapi; Tue, 11 Jan 2011 21:15:53 -0500 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500 6, 28 -- Subject: Staining somatic cells in milk 6, 28 -- Thread-Topic: Staining somatic cells in milk 6, 28 -- Thread-Index: Acux/qIfNhwwnZONaES65KSOZh3WKg== 6, 28 -- Message-ID: {C9527907.AFE6%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0C2Fs9b032153 ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From nizets2-at-yahoo.com Wed Jan 12 05:50:11 2011 25, 37 -- Received: from nm26-vm0.bullet.mail.sp2.yahoo.com (nm26-vm0.bullet.mail.sp2.yahoo.com [98.139.91.230]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0CBoBCs008689 25, 37 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 05:50:11 -0600 25, 37 -- Received: from [98.139.91.66] by nm26.bullet.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- Received: from [98.139.91.52] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- Received: from [127.0.0.1] by omp1052.mail.sp2.yahoo.com with NNFMP; 12 Jan 2011 11:50:10 -0000 25, 37 -- X-Yahoo-Newman-Property: ymail-3 25, 37 -- X-Yahoo-Newman-Id: 890075.12821.bm-at-omp1052.mail.sp2.yahoo.com 25, 37 -- Received: (qmail 70090 invoked by uid 60001); 12 Jan 2011 11:50:10 -0000 25, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1294833010; bh=dKrxBNFW4ytXLYCP+nF97/lTpfbjIbWO/CNk0eS6YLI=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=q2x0SSV6hKeozJ/R2FDsG3Be7Bc1cL/sVY/Q2jF+WrWZyl1mT/+Es349sXBn5Ej5NBlXIoGkwQO9jy1f/8w2K9SEpLT2O8kiRweASJ9QpAWZyii7gF13zKLgsw7Vg9dHMMMLHTuY2YSvDVkLX/inAA8QzvQaT9WNEWFVScRBz0A= 25, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 25, 37 -- s=s1024; d=yahoo.com; 25, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 25, 37 -- b=WTSHqBV9WqG6TdCWmU2Ukyvco5EtgGayejwD2oBbwtIAJwvw1Ur9SJO4JU1QMOVf7oTnvdANnGd9KopFMxzn6/0XLBmqU6ympJbPZrW8jc9A61gtLqro6+/0GY/gUEbfMchZ6lCQzUsdsoLtSnLfwK+/X6i/e51LwGTD3E6oOnA=; 25, 37 -- Message-ID: {218554.51777.qm-at-web110816.mail.gq1.yahoo.com} 25, 37 -- X-YMail-OSG: cMbsF_AVM1mSgmmzGAxvCz_JzlmJMeO2iKQLzx_I.kMfHoO 25, 37 -- MxtJ0BHEctYiA1dkyctHUsh.4mslgFpgRH5hDVrjWVC51aJidnclUQXItlgk 25, 37 -- mRWuBEErd5.7JQSZ8RjqYbiC5rFmEY7kXxJCEBQQ_LorYUopqnKzbfcLgjqv 25, 37 -- sTmqSi7Sveo6Rk7ixzVAGjjUz37IAjC3c6jihVk33zcUWc6VMd_pAi9hbQcW 25, 37 -- q3dkOAmBM9iC7VwgUud5W9S.Mwc_3P4vdz9R1dZeVOV4k8Q7SxAzZ01myUTG 25, 37 -- EMkG7XqdZgnQFvtXCZEyeYjD443V0tac8.8BF4qZpFFdoKvbHmqXQzfMr_7s 25, 37 -- UDR_jLSbGKPZ6N3HgtEoPauU1G2My2DfhxJEhye4VpNfY14m2CjXHlYkL2IV 25, 37 -- 6orYLno7cZQFrLWo- 25, 37 -- Received: from [80.122.101.100] by web110816.mail.gq1.yahoo.com via HTTP; Wed, 12 Jan 2011 03:50:09 PST 25, 37 -- X-Mailer: YahooMailRC/553 YahooMailWebService/0.8.107.285259 25, 37 -- References: {201101120218.p0C2Ii52009748-at-ns.microscopy.com} 25, 37 -- Date: Wed, 12 Jan 2011 03:50:09 -0800 (PST) 25, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 25, 37 -- Subject: Re: [Microscopy] Staining somatic cells in milk 25, 37 -- To: dsherman-at-purdue.edu 25, 37 -- Cc: microscopy-at-microscopy.com 25, 37 -- In-Reply-To: {201101120218.p0C2Ii52009748-at-ns.microscopy.com} 25, 37 -- MIME-Version: 1.0 25, 37 -- Content-Type: text/plain; charset=iso-8859-1 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CBoBCs008689 ==============================End of - Headers==============================
My mistake, I meant mechanical, Porter-Blum MTI was indeed mechanical, but you get the point. What is the name of Ruska's book that you mentioned.
Best,
Neeraj.
-----Original Message----- X-from: schooley-at-mcn.org [mailto:schooley-at-mcn.org] Sent: Wednesday, January 12, 2011 2:27 PM To: Neeraj Gohad
Sorry, Neera - the Porter-Blum microtome was mechanical advance. Simple, reliable, unbreakable.
Caroline
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 3, 18 -- From schooley-at-mcn.org Wed Jan 12 13:22:17 2011 3, 18 -- Received: from smtp1.mcn.org (smtp1.mcn.org [216.150.240.85]) 3, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJMGbL022762 3, 18 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:22:17 -0600 3, 18 -- Received: from [66.81.41.72] 3, 18 -- by smtp1.mcn.org with esmtpa (Exim 4.63) 3, 18 -- (envelope-from {schooley-at-mcn.org} ) 3, 18 -- id 1Pd6Gq-0007FY-56; Wed, 12 Jan 2011 11:22:13 -0800 3, 18 -- Mime-Version: 1.0 3, 18 -- Message-Id: {a06200706c953adc1f12a-at-[66.81.41.72]} 3, 18 -- In-Reply-To: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- References: {201101121910.p0CJAjKW017068-at-ns.microscopy.com} 3, 18 -- Date: Wed, 12 Jan 2011 11:21:19 -0800 3, 18 -- To: NEERAJG-at-clemson.edu 3, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 3, 18 -- Subject: Re: [Microscopy] Ernst Ruska 3, 18 -- Cc: {microscopy-at-microscopy.com} 3, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
==============================Original Headers============================== 12, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 13:52:50 2011 12, 37 -- Received: from mailclient1.clemson.edu (mailclient1.clemson.edu [130.127.237.180]) 12, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CJqoGn032605 12, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 13:52:50 -0600 12, 37 -- Received: from mailclient1.clemson.edu (localhost.clemson.edu [127.0.0.1]) 12, 37 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJqo9Z016883 12, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:52:50 -0500 12, 37 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 12, 37 -- by mailclient1.clemson.edu (8.13.8/8.13.8) with ESMTP id p0CJqnwV016874 12, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 12, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:52:49 -0500 12, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 12, 37 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 12, 37 -- 12 Jan 2011 14:52:49 -0500 12, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 12, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 12, 37 -- Date: Wed, 12 Jan 2011 14:52:47 -0500 12, 37 -- Subject: RE: [Microscopy] Re: Ernst Ruska 12, 37 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 12, 37 -- Thread-Index: Acuyjrj9EOFUj3n9TsK7feja0s9AqQAA1l3w 12, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562C03-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 12, 37 -- References: {201101121927.p0CJR8Vl002739-at-ns.microscopy.com} 12, 37 -- In-Reply-To: {201101121927.p0CJR8Vl002739-at-ns.microscopy.com} 12, 37 -- Accept-Language: en-US 12, 37 -- Content-Language: en-US 12, 37 -- X-MS-Has-Attach: 12, 37 -- X-MS-TNEF-Correlator: 12, 37 -- acceptlanguage: en-US 12, 37 -- Content-Type: text/plain; charset="us-ascii" 12, 37 -- MIME-Version: 1.0 12, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 12, 37 -- definitions=2011-01-12_05:2011-01-12,2011-01-12,1970-01-01 signatures=0 12, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 12, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 12, 37 -- engine=5.0.0-1012030000 definitions=main-1101120108 12, 37 -- Content-Transfer-Encoding: 8bit 12, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CJqoGn032605 ==============================End of - Headers==============================
The Electron Microscopy Core (EMC) at the University of Missouri Columbia, MO is proud to host an intensive short course:
Understanding and Optimizing FESEM and EDS Performance (Basic to Advanced)
Course Dates: Tuesday March 29 – Friday April 1, 2011 Cost: $1200 includes all course material (handouts and CD) and hands-on instrument instruction along with a reception and banquet Enrollment is limited to 12 participants!
Course Instructor: Steve Chapman - Protrain Steve Chapman formed Protrain in 1982 and is the senior consultant in electron microscopy. Steve first became involved with electron microscopes in 1964 at the University of Birmingham, England, then as an EM service engineer in the UK, and afterwards moved into sales and marketing where he worked with a number of electron microscope and accessory manufacturers in the demonstration and application fields. Running courses in many parts of the world, north and south of the equator, he has written six books including those on the operation and maintenance of scanning and transmission electron microscopes. His publications include papers on a range of electron microscopy subjects from instrument design through a wide range of applications and more recently on Quality in Electron Microscopy.
In a round-table illustrated discussion, you will be taken through the theory and practice of the operation of the scanning electron microscope, from basic through to advanced levels. In addition, the course will cover specimen preparation techniques and coating procedures. The EMC houses two field emission SEM’s, a Hitachi cold-field SEM (S-4700) and a FEI thermal FE SEM (Quanta 600 ESEM) with a Thermo System Six EDS. Practical periods will offer you the opportunity to evaluate instrument performance over a wide range of accelerating voltages and make appropriate modifications to the set-up in order to optimize the instrument’s performance. In this way, gun and specimen geometry may be better understood and you will be able to raise your skill levels to bring better performance from an instrument than the manufacturer claims. Other topics to be covered are low vacuum operation and imaging along with qualitative EDS analysis.
All students will operate the instruments themselves and develop their own talents. Under the eagle eye and guidance of Steve, participants should come away from this course with the understanding and abilities necessary to obtain the highest quality imaging and chemical analysis from their SEM/EDS systems.
Additional Support by: FEI, Hitachi High Technologies America, and Thermo Scientific.
For more Information or to register go to: http://www.emc.missouri.edu/ or contact me at rosslm-at-missouri.edu or at (573) 882-4777
Lou Ross -- Sr. Research Specialist University of Missouri Electron Microscopy Core Facility W136 Veterinary Medicine Building Columbia, MO 65211 573.882.4777, fax 573.884.2227 RossLM-at-missouri.edu http://www.emc.missouri.edu/
==============================Original Headers============================== 11, 31 -- From RossLM-at-missouri.edu Wed Jan 12 14:41:42 2011 11, 31 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CKfg6n019880 11, 31 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 14:41:42 -0600 11, 31 -- X-IronPort-Anti-Spam-Filtered: true 11, 31 -- X-IronPort-Anti-Spam-Result: AvsEAECgLU3RauUo/2dsb2JhbACkP3O2AIYYgw6CPgSEaIlO 11, 31 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.229.40]) 11, 31 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 12 Jan 2011 14:41:38 -0600 11, 31 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 11, 31 -- Wed, 12 Jan 2011 14:41:38 -0600 11, 31 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 11, 31 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Wed, 12 Jan 2011 11, 31 -- 14:41:37 -0600 11, 31 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 11, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 31 -- Date: Wed, 12 Jan 2011 14:41:36 -0600 11, 31 -- Subject: 4-Day SEM Training Course - Univ of Missouri 3/29/11 11, 31 -- Thread-Topic: 4-Day SEM Training Course - Univ of Missouri 3/29/11 11, 31 -- Thread-Index: AcuymRrTj+6+mUpTJUalkakpihLZgw== 11, 31 -- Message-ID: {C9536E20.18C00%rosslm-at-missouri.edu} 11, 31 -- Accept-Language: en-US 11, 31 -- Content-Language: en-US 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 31 -- acceptlanguage: en-US 11, 31 -- Content-Type: text/plain; charset="Windows-1252" 11, 31 -- MIME-Version: 1.0 11, 31 -- X-OriginalArrivalTime: 12 Jan 2011 20:41:38.0272 (UTC) FILETIME=[1C2E2E00:01CBB299] 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0CKfg6n019880 ==============================End of - Headers==============================
To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize.
Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said:
"Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre.
Paul Hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:34:36 2011 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNYaRn008589 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:34:36 -0600 11, 20 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 11, 20 -- (user=hazeltn mech=LOGIN bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNYZt9015594; 11, 20 -- Wed, 12 Jan 2011 17:34:35 -0600 (CST) 11, 20 -- Message-ID: {4D2E3A92.9060205-at-umanitoba.ca} 11, 20 -- Date: Wed, 12 Jan 2011 17:34:42 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Ernst Ruska 11, 20 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
The title of the book is The early development of electron lenses and electron microscopy. The English translation was by Mulvey, and it was published in 1980.
My copy was borrowed 25 years ago, and I have not seen it since. It is long out of print.
paul hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 4, 21 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:55:09 2011 4, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNt95v024544 4, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:55:09 -0600 4, 21 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 4, 21 -- (user=hazeltn mech=LOGIN bits=0) 4, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNt1f1024704; 4, 21 -- Wed, 12 Jan 2011 17:55:02 -0600 (CST) 4, 21 -- Message-ID: {4D2E3F5C.8060704-at-umanitoba.ca} 4, 21 -- Date: Wed, 12 Jan 2011 17:55:08 -0600 4, 21 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 4, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 4, 21 -- MIME-Version: 1.0 4, 21 -- To: NEERAJG-at-clemson.edu 4, 21 -- CC: Microscopy Listserver {microscopy-at-microscopy.com} 4, 21 -- Subject: Re: [Microscopy] Ernst Ruska 4, 21 -- References: {201101121953.p0CJrw53002740-at-ns.microscopy.com} 4, 21 -- In-Reply-To: {201101121953.p0CJrw53002740-at-ns.microscopy.com} 4, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 21 -- Content-Transfer-Encoding: 7bit 4, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brad-at-nanounity.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: brad-at-nanounity.com Name: Brad Rangell
Organization: NCSM
Title-Subject: [Filtered] NCSM kick-off meeting
Message: We are happy to announce the long anticipated reinstatement of the Northern California Chapter of the Society for Microscopy. Our first meeting will be an informal reception for you to get re-acquainted with your microscopy colleagues. During this get-together we want to begin to formalize membership and to obtain ideas for upcoming events for 2011. We anticipate the meeting to be a mix of networking and socializing, exchange of information and input from the membership on objectives and direction for this chapter. With this in mind here is the initial outline of the meeting. ...
Time: 6:00 to 8:00 PM
Location: International Building, SRI International, 333 Ravenswood Ave., Menlo Park, CA 94025; directions to follow.
Social hours: 6:00 to 7:00 PM hors de' oeuvres and beverages provided
Business items: 7:00 ñ 8:00 Informal Discussion of the following topics:
ï Officer Elections ñ we would like to ask members to provide suggestions of individuals to become elected officers. These can be submitted during the course of the evening. ï Discussion of By Laws for the society ï Discussion of future meetings and events in 2011 including subject matter and locations
Please send RSVP and questions to: Steve Samuelsson steven.samuelsson-at-sri.com or Brad Rangell brad-at-nanounity.com.
Please forward this notice to friends and colleagues who may have interest in joining NCSM and attending this kick-off meeting.
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D2Tt4S026512 for {MicroscopyListserverArchive2-at-microscopy.com} ; Wed, 12 Jan 2011 20:29:55 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p0D2TtRD026510; Wed, 12 Jan 2011 20:29:55 -0600
Paul,
Thanks for sharing the story as well as the title of the book. The story is really interesting and sheds some light on why the prize was awarded so late. As we are all aware, any human endeavor is fraught with politics and I am sure the Nobel committees aren't immune to it. On the subject of Nobel piece prizes, a pretty major one they missed (according to the Nobel foundation itself) was Mahatma Gandhi (if any ones interested http://nobelprize.org/nobel_prizes/peace/articles/gandhi/ ).
Best Regards,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Wednesday, January 12, 2011 6:40 PM To: Neeraj Gohad
Neeraj et al
To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize.
Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said:
"Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre.
Paul Hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:34:36 2011 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNYaRn008589 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:34:36 -0600 11, 20 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 11, 20 -- (user=hazeltn mech=LOGIN bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNYZt9015594; 11, 20 -- Wed, 12 Jan 2011 17:34:35 -0600 (CST) 11, 20 -- Message-ID: {4D2E3A92.9060205-at-umanitoba.ca} 11, 20 -- Date: Wed, 12 Jan 2011 17:34:42 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Ernst Ruska 11, 20 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 27, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 20:29:55 2011 27, 37 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 27, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D2TtRS026507 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:29:55 -0600 27, 37 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ttmu009634 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:55 -0500 27, 37 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ts5P009631 27, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:54 -0500 27, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 27, 37 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 27, 37 -- 12 Jan 2011 21:29:54 -0500 27, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 27, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 27, 37 -- Date: Wed, 12 Jan 2011 21:29:52 -0500 27, 37 -- Subject: RE: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Index: AcuysgUL2KFjAoUKSV6R1Ju/qF+IfAAFfX7Q 27, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562D32-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 27, 37 -- References: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- In-Reply-To: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- Accept-Language: en-US 27, 37 -- Content-Language: en-US 27, 37 -- X-MS-Has-Attach: 27, 37 -- X-MS-TNEF-Correlator: 27, 37 -- acceptlanguage: en-US 27, 37 -- Content-Type: text/plain; charset="us-ascii" 27, 37 -- MIME-Version: 1.0 27, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 27, 37 -- definitions=2011-01-13_01:2011-01-12,2011-01-13,1970-01-01 signatures=0 27, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 27, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 27, 37 -- engine=5.0.0-1012030000 definitions=main-1101120181 27, 37 -- Content-Transfer-Encoding: 8bit 27, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0D2TtRS026507 ==============================End of - Headers==============================
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Paul,
Thanks for sharing the story as well as the title of the book. The story is really interesting and sheds some light on why the prize was awarded so late. As we are all aware, any human endeavor is fraught with politics and I am sure the Nobel committees aren't immune to it. On the subject of Nobel piece prizes, a pretty major one they missed (according to the Nobel foundation itself) was Mahatma Gandhi (if any ones interested http://nobelprize.org/nobel_prizes/peace/articles/gandhi/ ).
Best Regards,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Wednesday, January 12, 2011 6:40 PM To: Neeraj Gohad
Neeraj et al
To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize.
Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said:
"Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre.
Paul Hazelton
Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982
==============================Original Headers============================== 11, 20 -- From paul_hazelton-at-umanitoba.ca Wed Jan 12 17:34:36 2011 11, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0CNYaRn008589 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 17:34:36 -0600 11, 20 -- Received: from [192.168.100.100] (wnpgmb014ww-ad06-64-92.dynamic.mts.net [207.161.64.92]) 11, 20 -- (user=hazeltn mech=LOGIN bits=0) 11, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0CNYZt9015594; 11, 20 -- Wed, 12 Jan 2011 17:34:35 -0600 (CST) 11, 20 -- Message-ID: {4D2E3A92.9060205-at-umanitoba.ca} 11, 20 -- Date: Wed, 12 Jan 2011 17:34:42 -0600 11, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 11, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 11, 20 -- MIME-Version: 1.0 11, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 11, 20 -- Subject: Re: [Microscopy] Ernst Ruska 11, 20 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- In-Reply-To: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} 11, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 20 -- Content-Transfer-Encoding: 7bit 11, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 27, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 20:29:55 2011 27, 37 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 27, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D2TtRS026507 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:29:55 -0600 27, 37 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ttmu009634 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:55 -0500 27, 37 -- Received: from frontex-3.CAMPUS.CU.CLEMSON.EDU (frontex-3.clemson.edu [130.127.235.57]) 27, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p0D2Ts5P009631 27, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 27, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 21:29:54 -0500 27, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 27, 37 -- frontex-3.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb39::827f:eb39]) with mapi; Wed, 27, 37 -- 12 Jan 2011 21:29:54 -0500 27, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 27, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 27, 37 -- Date: Wed, 12 Jan 2011 21:29:52 -0500 27, 37 -- Subject: RE: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Topic: [Microscopy] Re: Ernst Ruska 27, 37 -- Thread-Index: AcuysgUL2KFjAoUKSV6R1Ju/qF+IfAAFfX7Q 27, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938562D32-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 27, 37 -- References: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- In-Reply-To: {201101122339.p0CNdoha016514-at-ns.microscopy.com} 27, 37 -- Accept-Language: en-US 27, 37 -- Content-Language: en-US 27, 37 -- X-MS-Has-Attach: 27, 37 -- X-MS-TNEF-Correlator: 27, 37 -- acceptlanguage: en-US 27, 37 -- Content-Type: text/plain; charset="us-ascii" 27, 37 -- MIME-Version: 1.0 27, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 27, 37 -- definitions=2011-01-13_01:2011-01-12,2011-01-13,1970-01-01 signatures=0 27, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 27, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 27, 37 -- engine=5.0.0-1012030000 definitions=main-1101120181 27, 37 -- Content-Transfer-Encoding: 8bit 27, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0D2TtRS026507 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 28 -- From cross-at-tru.ca Wed Jan 12 22:20:08 2011 34, 28 -- Received: from mailgate.tru.ca (mailgate.tru.ca [192.146.156.111]) 34, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0D4K7r9011431 34, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 22:20:07 -0600 34, 28 -- Received: from mailgate.tru.ca (localhost.localdomain [127.0.0.1]) 34, 28 -- by mailgate (Postfix) with SMTP id 2E1E93246BF 34, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:20:05 -0800 (PST) 34, 28 -- Received: from Groupwise4.tru.ca (groupwise4.tru.ca [192.146.156.118]) 34, 28 -- by mailgate.tru.ca (Postfix) with ESMTP id 96EBB3246FE 34, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Jan 2011 20:20:04 -0800 (PST) 34, 28 -- Received: from TRUDOM4-MTA by Groupwise4.tru.ca 34, 28 -- with Novell_GroupWise; Wed, 12 Jan 2011 20:20:04 -0800 34, 28 -- Message-Id: {4D2E0CD9.BD8E.00AA.0-at-tru.ca} 34, 28 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 34, 28 -- Date: Wed, 12 Jan 2011 20:19:59 -0800 34, 28 -- From: "Cynthia Ross Friedman" {cross-at-tru.ca} 34, 28 -- To: {microscopy-at-microscopy.com} 34, 28 -- Subject: Re: [Microscopy] Ernst Ruska 34, 28 -- References: {201101130239.p0D2dCX7008495-at-ns.microscopy.com} 34, 28 -- In-Reply-To: {201101130239.p0D2dCX7008495-at-ns.microscopy.com} 34, 28 -- Mime-Version: 1.0 34, 28 -- Content-Type: text/plain; charset=US-ASCII 34, 28 -- Content-Disposition: inline 34, 28 -- X-PMX-Version: 5.5.5.374460, Antispam-Engine: 2.7.1.369594, Antispam-Data: 2011.1.13.40914 34, 28 -- X-PerlMx-Spam: Gauge=XII, Probability=12%, Report=' 34, 28 -- __CP_URI_IN_BODY! 0.5, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, DATE_TZ_NA 0, FROM_NAME_PHRASE 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P1_5 0, __C230066_P5 0, __CD 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_BADTHINGS 0, __FRAUD_CONTACT_NUM 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0' 34, 28 -- Content-Transfer-Encoding: 8bit 34, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0D4K7r9011431 ==============================End of - Headers==============================
I've collected some light brown low density materials (of size about 1cm3) from cultural heritage object that mixed with clay. I've done FTIR and identified the material to be cellulose/wood. It was dispersed in water and then mount on microscopic glass slide. The particles are irregular in shape and measured 45-75 microns. I highly suspected they were termite frass. Is there any reference guide for identification of termite frass? They are coming from subtropical region in South East asia, that is Hong Kong. Thank you.
Regards, Wing Fai
This email message (together with any attachments) is for the designated recipient only. It may contain information that is privileged for the designated recipient. If you are not the intended recipient, you are hereby notified that any use, retention, disclosure, copying, printing, forwarding or dissemination of the message is strictly prohibited. If you have received the message in error, please erase all copies of the message (including attachments) from your system and notify the sender immediately.
==============================Original Headers============================== 4, 25 -- From wflai-at-lcsd.gov.hk Thu Jan 13 03:15:39 2011 4, 25 -- Received: from sms01.lcsd.gov.hk (sms01.lcsd.gov.hk [203.31.32.70]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0D9FcuW001467 4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 03:15:39 -0600 4, 25 -- Received: from sms01.lcsd.gov.hk (unknown [127.0.0.1]) 4, 25 -- by sms01.lcsd.gov.hk (Symantec Mail Security) with ESMTP id B352985801D 4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:15:36 +0800 (HKT) 4, 25 -- X-AuditID: c0a80333-ad750bb000000a3a-19-4d2ec2b89451 4, 25 -- Received: from mail1.lcsd.gov.hk (unknown [10.47.1.65]) 4, 25 -- by sms01.lcsd.gov.hk (Symantec Mail Security) with ESMTP id 977607B8001 4, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:15:36 +0800 (HKT) 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- MIME-Version: 1.0 4, 25 -- Subject: LM need help to identify termite frass 4, 25 -- X-KeepSent: DDDD9E4E:C0D7718D-48257817:00326434; 4, 25 -- type=4; name=$KeepSent 4, 25 -- X-Mailer: Lotus Notes Release 8.5.1 FP2 March 18, 2010 4, 25 -- Message-ID: {OFDDDD9E4E.C0D7718D-ON48257817.00326434-48257817.0032DDB1-at-lcsd.gov.hk} 4, 25 -- From: wflai-at-lcsd.gov.hk 4, 25 -- Date: Thu, 13 Jan 2011 17:15:31 +0800 4, 25 -- X-MIMETrack: Serialize by Router on notes_svr_2/SERVERS/LCSD/HKSARG(Release 8.5.1FP5|September 4, 25 -- 29, 2010) at 13.01.2011 05:15:36 PM, 4, 25 -- Serialize complete at 13.01.2011 05:15:36 PM 4, 25 -- Content-Type: text/plain; charset="US-ASCII" 4, 25 -- X-Brightmail-Tracker: AAAAAA== ==============================End of - Headers==============================
} Date: Thu, 13 Jan 2011 11:32:22 -0800 (PST) } From: Ronald Schwartz {ronmschwartz-at-yahoo.com} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Thursday, January 13, } 2011 at 11:32:20 AM. } } realname - Ronald Schwartz } Email - ronmschwartz-at-yahoo.com } ORGANIZATION - Providence, RI museum, US Fish&Wildlife Nature Center } EDUCATION - 6-8th Grade Middle School } LOCATION - Wakefield, RI } SUBJECT_OF_QUESTION - increasing microscope activities } QUESTION - I am a volunteer at the museum and the nature center. I } have been trying to initiate and expand microscope activities. I } volunteer my own equipment and my time. I would like to expand this } activity both where I volunteer and in the schools, but the } equipment is limited. Are there places that donate microscopes or } other equipment like cameras that link between the microscope and a } computer? The schools, esp. the middle schools, have little or no } equipment for either science class and/or extra-curricular activity. } When there are old scopes, teachers also don't seem to be trained in } using them.
-- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 2, 23 -- From oshel1pe-at-cmich.edu Thu Jan 13 13:46:26 2011 2, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DJkQkd002966 2, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 13:46:26 -0600 2, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 2, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0DJkJHK004452 2, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 14:46:26 -0500 2, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 2, 23 -- Thu, 13 Jan 2011 14:46:23 -0500 2, 23 -- Mime-Version: 1.0 2, 23 -- Message-Id: {a06240803c95506cb3a73-at-[141.209.160.249]} 2, 23 -- Date: Thu, 13 Jan 2011 14:46:18 -0500 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 23 -- Subject: Ask-A-Microscopist - donor 'scopes for schools? 2, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 2, 23 -- X-OriginalArrivalTime: 13 Jan 2011 19:46:23.0644 (UTC) FILETIME=[8EEBD5C0:01CBB35A] 2, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 2, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 23 -- X-CanItPRO-Stream: default 2, 23 -- X-Canit-Stats-ID: 02DT7Kpgw - 80b0de5056a9 - 20110113 2, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Anyone know of a source for these? I'm teaching an microscopy course and I'd like to show this classic demonstration. I got some cheap crystals from Carolina Biological but they aren't very transparent.
Gary Radice Department of Biology University of Richmond Richmond VA 23173 804-289-8107
==============================Original Headers============================== 4, 23 -- From gradice-at-richmond.edu Thu Jan 13 14:07:12 2011 4, 23 -- Received: from nylon.richmond.edu (nylon.richmond.edu [141.166.30.20]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DK7BvU021921 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 14:07:12 -0600 4, 23 -- Received: from polyester.richmond.edu (polyester.richmond.edu [141.166.24.28]) 4, 23 -- by nylon.richmond.edu (8.13.8/8.13.8) with ESMTP id p0DK7BSL010714 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 15:07:11 -0500 4, 23 -- Received: from ffa186132.richmond.edu (ffa186132.richmond.edu [141.166.186.132]) 4, 23 -- by polyester.richmond.edu (8.13.8/8.13.8) with ESMTP id p0DK79fl014015 4, 23 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 15:07:10 -0500 4, 23 -- Message-Id: {2F16F0CF-B866-446B-9628-314505BAC684-at-richmond.edu} 4, 23 -- From: gradice {gradice-at-richmond.edu} 4, 23 -- To: Microscopy-at-microscopy.com 4, 23 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- Mime-Version: 1.0 (Apple Message framework v936) 4, 23 -- Subject: Source of calcite crystals for demonstrating birefringence? 4, 23 -- Date: Thu, 13 Jan 2011 15:07:09 -0500 4, 23 -- X-Mailer: Apple Mail (2.936) 4, 23 -- X-Spam-Detail: 0 4, 23 -- X-Scanned-By: MIMEDefang 2.67 on 141.166.24.28 ==============================End of - Headers==============================
A geology department will usually have transparent calcite on hand, but your institution does not appear to have that program. Transparent calcite is often referred to as "Iceland Spar." A quick search should turn up several potential suppliers. Wards, for example, has specimens in four different size/quality categories with prices that vary accordingly.
Regards,
- Steve Kuehn
gradice-at-richmond.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Anyone know of a source for these? I'm teaching an microscopy course } and I'd like to show this classic demonstration. I got some cheap } crystals from Carolina Biological but they aren't very transparent. } } } Gary Radice } Department of Biology } University of Richmond } Richmond VA 23173 } 804-289-8107
--
---------------- Dr. Stephen C. Kuehn Research Scientist and Lecturer Manager, Electron Microprobe Facility Division of Natural Sciences Science, room 106
Concord University, Campus Box F20 1000 Vermillion St PO Box 1000 Athens, WV 24712-1000
Bart Cannon {cannonmp-at-comcast.net} just asked me to pass along that he has available plenty of inexpensive, colorless "iceland spar" suitable for demonstration purposes.
Regards,
- Steve
} } Gary, } } A geology department will usually have transparent calcite on hand, but } your institution does not appear to have that program. Transparent } calcite is often referred to as "Iceland Spar." A quick search should } turn up several potential suppliers. Wards, for example, has specimens } in four different size/quality categories with prices that vary } accordingly. } } Regards, } } - Steve Kuehn
--
---------------- Dr. Stephen C. Kuehn Research Scientist and Lecturer Manager, Electron Microprobe Facility Division of Natural Sciences Science, room 106
Concord University, Campus Box F20 1000 Vermillion St PO Box 1000 Athens, WV 24712-1000
So I did a little digging on who reported thermal advance in ultramicrotomes first (not which commercial instruments came first) the original paper by Keith Porter and J. Blum published in 1953, A study in Microtomy for electron Microscopy, The Anatomical Record Volume 117, Issue 4, pages 685-709, December 1953, in it Porter and Blum describe two new mechanisms, one is a thermal expansion and the other is mechanical advancement (I have a PDF of this paper if anyone is interested). Here is an excerpt from the Summary of this paper
'Two new microtomes capable of cutting serial sections as thin as 25-50 mu are described. All moving parts in these instruments are supported in unlubricated pivots and the specimen is taken past the cutting edge only on the cutting stroke. These two features more than any others seem responsible for the successful performance of these microtomes. In one instrument, the prototype, the block is advanced to the knife by thermal expansion. In the other, the advancement is controlled mechanically.'
Interestingly enough, Fritiof Sjostrand's paper, A new microtome for ultrathin sectioning for high resolution microscopy, 1953 Experientia 9:114-121 (note the paper also published in 1953) says 'The microtome reported on in this paper represents a further development of the microtome designed by Porter' (http://www.springerlink.com/content/y3267k5456j11117/).
Also found an interesting book, Picture Control: The Electron Microscope and the Transformation of Biology in America by Nicolas Rasmussen, has some interesting history on this matter (see preview, http://books.google.com/books?id=rwC5QiqLS44C&pg=PA127&lpg=PA127&dq=Sjostrand+a+new+ultramicrotome&source=bl&ots=HCTXlObRJ-&sig=G46YfqxY8lrFtBptZDgmxIDbxuA&hl=en&ei=cHAvTfLRBsKSgQfMo-xa&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCwQ6AEwAg#v=onepage&q&f=false
Pretty Interesting!
Best,
Neeraj.
Neeraj V. Gohad, Ph.D. Postdoctoral Fellow Okeanos Research Group Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
Website: http://www.clemson.edu/okeanos
X-from: Paul Hazelton [mailto:hazeltn-at-cc.umanitoba.ca] Sent: Wednesday, January 12, 2011 4:09 PM To: Neeraj Gohad
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Ron -
Bravo for your efforts! Are you aware that 1) MSA has regional societies, & 2) the New England Society has kits for middle school microscopy that they circulate to people like you? Their program chair is Mary McCann {mccanns-at-tiac.net} , (617)484-7865. If you have difficulty reaching her, let me know.
I hope that you'll visit the Project MICRO web page (URL below). There's a lot of advice about suitable equipment. Donated scopes can be more of a liability than an asset; they are too complex for middle school teachers & they usually need parts & service.
We're here to help; please ask questions. I'm a retiree, which means that I have the time to respond to your Email!
Caroline -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 6, 18 -- From schooley-at-mcn.org Thu Jan 13 17:49:32 2011 6, 18 -- Received: from smtp2.mcn.org (smtp2.mcn.org [216.150.240.86]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DNnW28025218 6, 18 -- for {microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:49:32 -0600 6, 18 -- Received: from [66.81.50.60] 6, 18 -- by smtp2.mcn.org with esmtpa (Exim 4.63) 6, 18 -- (envelope-from {schooley-at-mcn.org} ) 6, 18 -- id 1PdWuz-0001Du-4D; Thu, 13 Jan 2011 15:49:26 -0800 6, 18 -- Mime-Version: 1.0 6, 18 -- Message-Id: {a06200702c9553af52ab6-at-[66.81.43.189]} 6, 18 -- In-Reply-To: {201101131956.p0DJuH7j015484-at-ns.microscopy.com} 6, 18 -- References: {201101131956.p0DJuH7j015484-at-ns.microscopy.com} 6, 18 -- Date: Thu, 13 Jan 2011 15:48:58 -0800 6, 18 -- To: oshel1pe-at-cmich.edu 6, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 6, 18 -- Subject: Re: [Microscopy] Ask-A-Microscopist - donor 'scopes for schools? 6, 18 -- Cc: microscopy-at-microscopy.com 6, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
We're in the process of writing a proposal for a new SEM, and will likely include a cathodoluminescence detector. As I don't know a whole lot about these, I'd like some input on performance, brands, models, prices, company service (especially service) and the like. Use will be for materials and especially mineralogy research and teaching in our microscopy program. So the unit needs to be suitable for research, but robust and user-friendly enough for teaching.
This is not for a NSF or such grant, so cost is also important (low cost, whatever that is for a good cathodoluminescence detector). But I don't have a good handle on prices yet - other than that there are some hideously expensive units that we can't consider. Vendor replies welcome.
BUT! **Off-line** please, not to the list.
Although if someone wants to start a discuss on the technique and uses of cathodoluminescence, I'd be interested in reading it ...
Thanks.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 23 -- From oshel1pe-at-cmich.edu Fri Jan 14 07:58:33 2011 7, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0EDwXFd013018 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 07:58:33 -0600 7, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0EDwJFD009286 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 08:58:33 -0500 7, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 23 -- Fri, 14 Jan 2011 08:57:31 -0500 7, 23 -- Mime-Version: 1.0 7, 23 -- Message-Id: {a06240804c95604cdfa23-at-[141.209.160.249]} 7, 23 -- Date: Fri, 14 Jan 2011 08:57:28 -0500 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 23 -- Subject: cathodoluminescence detectors 7, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 23 -- X-OriginalArrivalTime: 14 Jan 2011 13:57:32.0005 (UTC) FILETIME=[FD1AF150:01CBB3F2] 7, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 23 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 7, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 23 -- X-CanItPRO-Stream: default 7, 23 -- X-Canit-Stats-ID: 02DTpWxSy - c2f15745d688 - 20110114 7, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
First! Please reply directly to me, **not** the list.
We are hoping to get a new silicon drift detector (SDD) EDS system for our SEM. Either as a retro-fit or for a new SEM. I've been looking at the models available, but I'd like to get some user input - reliability, user-friendliness, company service, is the software open-source or proprietary, and how much does the company charge for software upgrades? EDS software integration with SEM software/hardware, energy resolution, light element abilities - all the usual things, but as actual user experiences.
Is the system tied to a particular computer hardware design? (Our current system uses an old proprietary circuit board that uses an obsolete slot/bus, so it can't be upgraded or moved to a new computer, the whole thing has to be replaced.)
This will be for both research - materials, mineralogy, biology, and whatever else comes up - and teaching.
Vendor replies welcome. To me, please, not the list.
Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 23 -- From oshel1pe-at-cmich.edu Fri Jan 14 08:08:02 2011 7, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0EE82HY025267 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 08:08:02 -0600 7, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0EE7eXR010183 7, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 09:08:02 -0500 7, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 23 -- Fri, 14 Jan 2011 09:06:44 -0500 7, 23 -- Mime-Version: 1.0 7, 23 -- Message-Id: {a06240803c95604c5f849-at-[141.209.160.249]} 7, 23 -- Date: Fri, 14 Jan 2011 09:06:41 -0500 7, 23 -- To: Microscopy-at-microscopy.com 7, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 23 -- Subject: SDD detectors 7, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 7, 23 -- X-OriginalArrivalTime: 14 Jan 2011 14:06:44.0801 (UTC) FILETIME=[4698EB10:01CBB3F4] 7, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 23 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 7, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 23 -- X-CanItPRO-Stream: default 7, 23 -- X-Canit-Stats-ID: 02DTq82WV - d70aee24b0f7 - 20110114 7, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
} Date: Fri, 14 Jan 2011 07:20:51 -0800 (PST) } From: Norah Silva {norah-at-norahsilva.com} } Subject: Ask-A-Microscopist - Photomicroscopy training in Florida? } } Below is the result of your form, submitted on Friday, January 14, } 2011 at 07:20:43 AM. } } realname - Norah Silva } Email - norah-at-norahsilva.com } EDUCATION - Undergraduate College } LOCATION - Boca Raton, FL 33432 } SUBJECT_OF_QUESTION - Capturing images } QUESTION - Hello, } I am a photographer and am going back to school for my BS and am } very interested in the field of microscopy as well as recomendations } of where to learn more about capture systems. Are there devices } compatible with Hasselblad or Canon digital capture systems? } What is the typical line of study to get in to this field? } } Thank you in advance for your advice, } Norah Silva } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 23 -- From oshel1pe-at-cmich.edu Fri Jan 14 12:01:41 2011 1, 23 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0EI1fG4005497 1, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 12:01:41 -0600 1, 23 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 23 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0EI1cwl001804 1, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 14 Jan 2011 13:01:41 -0500 1, 23 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 23 -- Fri, 14 Jan 2011 13:00:55 -0500 1, 23 -- Mime-Version: 1.0 1, 23 -- Message-Id: {a06240818c9563f9bc26e-at-[141.209.160.249]} 1, 23 -- Date: Fri, 14 Jan 2011 13:00:51 -0500 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 23 -- Subject: Fwd: Ask-A-Microscopist - Photomicroscopy training in Florida? 1, 23 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 23 -- X-OriginalArrivalTime: 14 Jan 2011 18:00:55.0574 (UTC) FILETIME=[FD82BB60:01CBB414] 1, 23 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 23 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 23 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 23 -- X-CanItPRO-Stream: default 1, 23 -- X-Canit-Stats-ID: 02DTu1FGE - d8187a12bc04 - 20110114 1, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
A post-doctoral position is available in the LeBeau group at North Carolina State University. The successful candidate will be responsible for the development of scanning transmission electron microscopy techniques in conjunction with studying interfaces, nanoparticles/nanocomposites, and device structures. The candidate will focus on quantitative electron microscopy, determination of heterogeneous interface structures, and exploring chemistry with spectroscopic techniques. Applicants should have demonstrated expertise in problem solving in materials science using a wide range of transmission electron microscopy techniques.
NCSU will soon acquire an aberration corrected microscope for ultra-high resolution STEM imaging and chemical analysis. Other equipment includes a JEOL 2010 STEM/TEM, several conventional TEMs, and a FIB. The position is available starting Jan 1, 2011 and requires a Ph. D. in materials science or a related field. Experience with aberration corrected STEM is desired. Duration is between 1-3 years and salary is commensurate with qualifications.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vish.bhakthavatsalam-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Microscopist with a phD in chemistry
Message: A minimum Ph.D. in Physical Sciences, Analytical Chemistry, Material Science or related discipline ï Experience A minimum of 2-3 years of Hands ñon instrument experience in SEM-EDS, EBSD, TEM are required in the industry. Experience with materials used in the mining/metal industry is a bonus; A background in mineralogy and hands on experience in characterization techniques thereof (SEM-EDS, EBSD, TEM, AFM, sputter coating, sample preparation using ultra microtome) is required. Experience in surface science processes and techniques (e.g. AFM, STM) will be considered especially advantageous.
ï Mandatory skills Good communication and presentation skills must be evident; peer review publications in international journals and presentations at significant analytical conferences is a plus. Erase this text and type your question here
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both erwrigh-at-emory.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: erwrigh-at-emory.edu Name: Elizabeth Wright
Organization: Emory University
Title-Subject: [Filtered] Postdoctoral Position Available
Message: A postdoctoral fellow position is available immediately with the Spearman and Wright laboratories at Emory University to study HIV assembly and trafficking using fluorescence microscopy and cryo-EM methods.
Motivated candidates with strong interests in HIV biology and cryo-electron microscopy of complex molecular assemblies are encouraged to apply. Research experience in the following area(s) is desired: 1) retrovirology, 2) cryo-EM, and/or 3) fluorescence microscopy.
Our laboratories are within the Division of Pediatric Infectious Diseases and are fully equipped for all aspects of molecular virology, molecular biology and protein biochemistry. The Spearman lab has a Deltavision deconvolution microscopy station and a spinning disk confocal system both equipped for live cell imaging, FRAP, photoactivation, and now for cryo-imaging. The Wright lab has complete access to a JEOL 2200FS 200 kV FEG TEM with an in-column energy filter, Zernike phase plates, and high-resolution 4kx4k CCD camera; a JEOL 1400 120 kV TEM with 2kx2k CCD camera; and an FEI Vitrobot and a manual grid plunge freezer. Emory is a vibrant, multidisciplinary campus with strong ties to Georgia Tech and other universities within Georgia. Access to facilities in NMR, Mass Spectrometry, and X-ray crystallography are available.
Applicants should include a cover letter describing research experience and interests, curriculum vitae, and names and contact information for three references.
Dr. Elizabeth R. Wright Emory University Department of Pediatrics 2015 Uppergate Drive, NE Room: 548 Atlanta, GA 30322 erwrigh-at-emory.edu Website: http://electronmicroscopy.emory.edu
Applications will be reviewed as they are received and until the position is filled.
We have an old CI-46 water jacketed CO2 incubator that was donated years ago. Someone wants to brush the dust off and try using this because they want to avoid contaminating our main incubators with something they want to grow.
Our problem though is that we no longer have contact with the group that donated the incubator I think, and they didn't include a manual. Does anyone have a manual laying around or basic instructions for use? None of the ports on the backside are labeled anymore, and I don't know if American Scientific Products got absorbed by another company I could possibly call or did they just dissolve or do some other action causing it to be a problem tracking them down. I'm a youngling.
On the backside I just see three ports, one is the power, one is something electrical, and the other looks like a water siphon port. I'm assuming the nut on the front when you open the door might be the fill for the water jacket. We don't see where the CO2 is attached anywhere and their is a rather larger hole through the back of the chamber and we don't see anything that should fill that hole given to us along with the incubator. I guess they were afraid the cells might be scared of the dark?
Help in either getting us an old manual or contact for who might still be servicing these machines would be helpful. Not quite microscopy but assuming a few people are still around biology labs or maybe a colleague they know. Thanks and enjoy the weekend.
Off topic for this, thanks for those of you on the Ernst Ruska thread. Some interesting informative posts about history are always a good read.
-Jason Saredy University of North Florida
==============================Original Headers============================== 7, 27 -- From sarj0007-at-unf.edu Sat Jan 15 13:02:12 2011 7, 27 -- Received: from relay4.unf.edu (relay4.unf.edu [139.62.192.127]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0FJ2Cm7006325 7, 27 -- for {microscopy-at-microscopy.com} ; Sat, 15 Jan 2011 13:02:12 -0600 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: AvsEAH59MU2LPsCK/2dsb2JhbACkaXO1aIZqhVAEhHA 7, 27 -- Received: from callisto1.unfcsd.unf.edu ([139.62.192.138]) 7, 27 -- by relay1.unf.edu with ESMTP; 15 Jan 2011 14:02:11 -0500 7, 27 -- Received: from jupiter.unfcsd.unf.edu ([169.254.1.119]) by 7, 27 -- callisto1.unfcsd.unf.edu ([139.62.192.138]) with mapi; Sat, 15 Jan 2011 7, 27 -- 14:02:11 -0500 7, 27 -- From: "Saredy, Jason" {sarj0007-at-unf.edu} 7, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 27 -- Date: Sat, 15 Jan 2011 14:02:10 -0500 7, 27 -- Subject: ci-46 incubator 7, 27 -- Thread-Topic: ci-46 incubator 7, 27 -- Thread-Index: AQHLtOa22xn9Zcp58UKhBJKltFcEiw== 7, 27 -- Message-ID: {68A0416A3B94A542B9164AE7A32DE1DE2F30B71F22-at-JUPITER.unfcsd.unf.edu} 7, 27 -- Accept-Language: en-US 7, 27 -- Content-Language: en-US 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- acceptlanguage: en-US 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0FJ2Cm7006325 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Michael.Cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Michael.Cammer-at-med.nyu.edu Name: Michael Cammer
Message: We have the Nikon TIRF system and have three laser lines going into the TIRF arm via a single fiber. When we project through the 100X objective through the sample onto the wall we see that the lines go through the sample at different angles. (You can see a picture of the projection at approx 45 degrees at http://www.flickr.com/photos/mcammer/5359189090/ .) It is also noticeable in the TIRF images that the field depth is different for each wavelength. Is this unavoidable due to the different wavelengths or is it possible to align the optics better so these spots would be more coincident?
That's a beautiful photograph of the unavoidable relationship between the angle of incidence and the wavelength of light used to illuminate the sample. It stems from the fact that the relative index of refraction of the glass-water interface is slightly different for for each wavelength. To ensure that the penetration depth is nearly equal for all laser lines, you would have to offset each laser beam to a different specific radial position on the back focal plane of the objective - and these relative spacings would change as well if you wanted a different penetration depth. You can work out what these spacings need to be by examining the basic equation for the TIRF penetration depth (you have to assume you know the wavelength dependent index of refraction of the sample though which ranges from 1.33 to 1.38) and you'll find that they are on the order of a few tens of micrometers depending on the specific penetration depth you desire (see http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfintro.html). There's a nice little ImageJ plugin that can be used:
http://rsbweb.nih.gov/ij/plugins/tirf/index.html
John Oreopoulos Research Assistant Spectral Applied Research 9078 Leslie Street, Unit 11 Richmond Hill, Ontario Canada
On 2011-01-16, at 10:37 AM, Michael.Cammer-at-med.nyu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both Michael.Cammer-at-med.nyu.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Michael.Cammer-at-med.nyu.edu } Name: Michael Cammer } } Organization: Skirball Langone NYU Med Center } } Title-Subject: [Filtered] TIRF laser alignment question } } Message: We have the Nikon TIRF system and have three laser lines } going into the TIRF arm via a single fiber. When we project through } the 100X objective through the sample onto the wall we see that the } lines go through the sample at different angles. (You can see a } picture of the projection at approx 45 degrees at } http://www.flickr.com/photos/mcammer/5359189090/ .) It is also } noticeable in the TIRF images that the field depth is different for } each wavelength. Is this unavoidable due to the different } wavelengths or is it possible to align the optics better so these } spots would be more coincident? } } Thank you. } } Sincerely, } } Michael Cammer } } Login Host: 96.246.240.120 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 9, 22 -- From zaluzec-at-microscopy.com Sun Jan 16 09:30:29 2011 } 9, 22 -- Received: from znl.com ([206.69.208.20]) } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0GFUTMI000864 } 9, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 09:30:29 -0600 } 9, 22 -- Received: from localhost (localhost [127.0.0.1]) } 9, 22 -- by znl.com (Postfix) with ESMTP id 9DE9B313DDB } 9, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 09:30:29 -0600 (CST) } 9, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 9, 22 -- Received: from znl.com ([127.0.0.1]) } 9, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 9, 22 -- with ESMTP id UiKkrOX5x+kx for {microscopy-at-microscopy.com} ; } 9, 22 -- Sun, 16 Jan 2011 09:30:29 -0600 (CST) } 9, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 9, 22 -- by znl.com (Postfix) with ESMTPA id CF970313DCB } 9, 22 -- for {microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 09:30:28 -0600 (CST) } 9, 22 -- Mime-Version: 1.0 } 9, 22 -- Message-Id: {p06240801c958bf8365ef-at-[206.69.208.22]} } 9, 22 -- Date: Sun, 16 Jan 2011 09:30:26 -0600 } 9, 22 -- To: microscopy-at-microscopy.com } 9, 22 -- From: Michael.Cammer-at-med.nyu.edu (by way of MicroscopyListserver) } 9, 22 -- Subject: viaWWW: TIRF laser alignment question } 9, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 28 -- From john.oreopoulos-at-utoronto.ca Sun Jan 16 10:30:17 2011 9, 28 -- Received: from bureau61.ns.utoronto.ca (bureau61.ns.utoronto.ca [128.100.132.151]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0GGUHLW018522 9, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 16 Jan 2011 10:30:17 -0600 9, 28 -- Received: from [192.168.2.13] (bas3-toronto06-2925097395.dsl.bell.ca [174.89.113.179] (may be forged)) 9, 28 -- (authenticated bits=0) 9, 28 -- by bureau61.ns.utoronto.ca (8.13.8/8.13.8) with ESMTP id p0GGUDK7017003 9, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO); 9, 28 -- Sun, 16 Jan 2011 11:30:15 -0500 9, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/simple; d=utoronto.ca; s=beta; 9, 28 -- t=1295195416; bh=LxPipE27XvQW9QQHRyw/C8uclP9Psf8CCabI44i6C7c=; 9, 28 -- h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date: 9, 28 -- Content-Transfer-Encoding:Message-Id:References:To; 9, 28 -- b=BIQ1TDiX05nv3YUpUiAQxh6KNAaUASTi5En1S0UvG31FM1WdIMcdQGbmepza/cppb 9, 28 -- AfuYv8I/F5YXbZ3ec5JwrDrbTZQphrWjpC64BhIAL3AQbG4XsdKDRcUYmXd3woLwpc 9, 28 -- 5OG5OeED1Nx9W84vIwEYWEZNYRS5aL9Bp5MXm+EA= 9, 28 -- Content-Type: text/plain; charset=us-ascii 9, 28 -- Mime-Version: 1.0 (Apple Message framework v1082) 9, 28 -- Subject: Re: [Microscopy] viaWWW: TIRF laser alignment question 9, 28 -- From: John Oreopoulos {john.oreopoulos-at-utoronto.ca} 9, 28 -- In-Reply-To: {201101161537.p0GFbWHh008327-at-ns.microscopy.com} 9, 28 -- Date: Sun, 16 Jan 2011 11:30:13 -0500 9, 28 -- Message-Id: {BA43080F-F9E9-4657-BBD0-84AF19314BF3-at-utoronto.ca} 9, 28 -- References: {201101161537.p0GFbWHh008327-at-ns.microscopy.com} 9, 28 -- To: Michael.Cammer-at-med.nyu.edu, Microscopy-at-microscopy.com 9, 28 -- X-Mailer: Apple Mail (2.1082) 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0GGUHLW018522 ==============================End of - Headers==============================
Thanks for the reply. Reading it and the referenced websites jogged my memory.
A few years ago we were having problems with the first commercial Olympus TIRF system because we could not get consistent evanescent waves with the one angle adjustment with the laser lines we had from 405 to 568 nm that were delivered via a single fiber (it was worse when we later added a 633 or 638 nm laser). I suggested we pump each laser in through a separate path that could be angled independently. We didn't build it, but I think Olympus now sells a TIRF system that does this.
Another issue is that when I first heard about TIRF maybe 15 years ago, it was introduced as a ring illumination at the outer edge of the back aperture, not as a single point or crescent at the periphery on only one side. A ring, or at least a series of points around the periphery, seems like a better way to provide a uniform field due to aberrations from coherent light in the imperfect optics. Any thought on this?
Sincerely,
Michael
_________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
________________________________________ X-from: John Oreopoulos [john.oreopoulos-at-utoronto.ca] Sent: Sunday, January 16, 2011 11:30 AM To: Cammer, Michael; Microscopy-at-microscopy.com
Dear Listers,
I am posting this on the request of Bill Caufman from Intersil Company in Palm Bay, Florida. Bill needs help with reviving an EDX system with "Voyager" electronics made by Noran and needs technical assistance.
If someone can provide technical help or even on-site assistance for this kind of EDX system in Palm Bay Florida - please respond directly to Bill at
BCAUFFMA-at-intersil.com
Thank you very much beforehand, -- Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com
==============================Original Headers============================== 5, 32 -- From vray-at-partbeamsystech.com Mon Jan 17 21:19:19 2011 5, 32 -- Received: from nm16-vm0.bullet.mail.ac4.yahoo.com (nm16-vm0.bullet.mail.ac4.yahoo.com [98.139.52.238]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0I3JIfD015751 5, 32 -- for {microscopy-at-microscopy.com} ; Mon, 17 Jan 2011 21:19:18 -0600 5, 32 -- Received: from [98.139.52.193] by nm16.bullet.mail.ac4.yahoo.com with NNFMP; 18 Jan 2011 03:19:18 -0000 5, 32 -- Received: from [98.139.52.142] by tm6.bullet.mail.ac4.yahoo.com with NNFMP; 18 Jan 2011 03:19:18 -0000 5, 32 -- Received: from [127.0.0.1] by omp1025.mail.ac4.yahoo.com with NNFMP; 18 Jan 2011 03:19:18 -0000 5, 32 -- X-Yahoo-Newman-Id: 235624.18271.bm-at-omp1025.mail.ac4.yahoo.com 5, 32 -- Received: (qmail 36405 invoked from network); 18 Jan 2011 03:19:17 -0000 5, 32 -- Received: from [172.18.10.172] (vray-at-211.72.229.241 with plain) 5, 32 -- by smtp112.biz.mail.mud.yahoo.com with SMTP; 17 Jan 2011 19:19:17 -0800 PST 5, 32 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 32 -- X-YMail-OSG: s0fHlvoVM1kvkA6GtZygbox2jxTjpzTZ8sm26XeoHvo.N20 5, 32 -- f.o8hb2_9BtxQ7DZAlmwTO1pi3lghMbrDwtRIfxRPNt3wsakSFTjReZWEXjX 5, 32 -- 09OgtrP6XtrsrFvekc5fTVsRsZEjZk5h4q2.N8ML0.ZjT2V7.bPrgEqlKrbt 5, 32 -- XLUYWd_J42r__31VEfYPZggeZwCxyj4bcABcKFDBgLf8laDY.8fuiAwMk1fs 5, 32 -- oAt_oq.DiafbxbG2mf8E5DeBdXSTCvUoD0s5vFEgbnG3J5KgkL72nhksQMO_ 5, 32 -- rsKWr1t01B41gMd3WEfOPMHoHRgPeu4mpTaklH0pe_bZ79Y84jMAG6gdCo8R 5, 32 -- XnJRKfaw6pS61eM.sM1ANFuZrIPkY_Bk3sAL5HK14Rpjn 5, 32 -- X-Yahoo-Newman-Property: ymail-3 5, 32 -- Message-ID: {4D3506B4.4060802-at-partbeamsystech.com} 5, 32 -- Date: Mon, 17 Jan 2011 22:19:16 -0500 5, 32 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 32 -- Reply-To: vray-at-partbeamsystech.com 5, 32 -- Organization: PBS&T 5, 32 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Lightning/1.0b2 Thunderbird/3.1.7 5, 32 -- MIME-Version: 1.0 5, 32 -- To: microscopy-at-microscopy.com 5, 32 -- CC: "Cauffman, Bill \(bcauffma\)" {BCAUFFMA-at-intersil.com} 5, 32 -- Subject: Help with Noran/Voyager EDX in Florida 5, 32 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Sun, 16 Jan 2011 19:19:44 +0100, {Michael.Cammer-at-med.nyu.edu} wrote:
} Another issue is that when I first heard about TIRF maybe 15 years ago, } it was introduced as a ring illumination at the outer edge of the back } aperture, not as a single point or crescent at the periphery on only one } side. A ring, or at least a series of points around the periphery, } seems like a better way to provide a uniform field due to aberrations } from coherent light in the imperfect optics. Any thought on this?
Yes, you are right. See e.g. this paper about this very topic: Fiolka, R., Belyaev, Y., Ewers, H., & Stemmer, A. (2008). Even illumination in total internal reflection fluorescence microscopy using laser light. Microscopy research and technique, 71(1), 45-50. doi: 10.1002/jemt.20527.
Cheers Janne
==============================Original Headers============================== 7, 19 -- From Janne.Hyoetylae-at-stud.unibas.ch Tue Jan 18 07:38:23 2011 7, 19 -- Received: from smtp2.unibas.ch (smtp2.unibas.ch [131.152.227.82]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0IDcMsH020835 7, 19 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 07:38:23 -0600 7, 19 -- X-IronPort-AV: E=Sophos;i="4.60,339,1291590000"; 7, 19 -- d="scan'208";a="117112964" 7, 19 -- Received: from bioz3-mihp55.mih.unibas.ch (HELO moria) ([131.152.19.124]) 7, 19 -- by smtp2priv.unibas.ch with ESMTP; 18 Jan 2011 14:38:13 +0100 7, 19 -- Content-Type: text/plain; charset=utf-8; format=flowed; delsp=yes 7, 19 -- To: Michael.Cammer-at-med.nyu.edu, Microscopy-at-microscopy.com 7, 19 -- Subject: Re: TIRF laser alignment question 7, 19 -- References: {201101161819.p0GIJilA016381-at-ns.microscopy.com} 7, 19 -- Date: Tue, 18 Jan 2011 14:38:12 +0100 7, 19 -- MIME-Version: 1.0 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- From: =?utf-8?Q?Janne_Hy=C3=B6tyl=C3=A4?= {Janne.Hyoetylae-at-stud.unibas.ch} 7, 19 -- Message-ID: {op.vpiatyrsxxlwia-at-moria} 7, 19 -- In-Reply-To: {201101161819.p0GIJilA016381-at-ns.microscopy.com} 7, 19 -- User-Agent: Opera Mail/11.00 (Linux) ==============================End of - Headers==============================
The Deben stage controls on the SEM are getting a little stiff, and the maintenance manual recommends Santovac 5 for lubrication. Too bad, I don't have any around, and I don't relish the thought of forking over several hundred dollars for an entire bottle just to use a miniscule bit. Has anybody out there recently serviced a DP and have an "empty" Santovac container that I could squeeze a few drops out of? I'll spring for shipping, plus offer sacrifices to the gods of EM on your behalf for good vibes and round electrons.
Thanks in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
If you don't get any from others in the Great White North, let me know, I can send you some. If we can get it past NTSA and Customs and ...
Phil Hm. "GWN" is somewhat more than the land of the Maple Leaf right now.
} Greetings listers, } } The Deben stage controls on the SEM are getting a little stiff, and the } maintenance manual recommends Santovac 5 for lubrication. Too bad, I } don't have any around, and I don't relish the thought of forking over } several hundred dollars for an entire bottle just to use a miniscule } bit. Has anybody out there recently serviced a DP and have an "empty" } Santovac container that I could squeeze a few drops out of? I'll spring } for shipping, plus offer sacrifices to the gods of EM on your behalf for } good vibes and round electrons. } } Thanks in advance, } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } Svent-Gyorgyi's Axiom: } Discovery consists of seeing what everybody } has seen and thinking what nobody thought.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 25 -- From oshel1pe-at-cmich.edu Tue Jan 18 14:18:36 2011 5, 25 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0IKIac0031751 5, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 14:18:36 -0600 5, 25 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 25 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0IKIW6v027588 5, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 15:18:35 -0500 5, 25 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 5, 25 -- Tue, 18 Jan 2011 15:18:35 -0500 5, 25 -- Mime-Version: 1.0 5, 25 -- Message-Id: {a06240808c95ba5ac95fb-at-[141.209.160.249]} 5, 25 -- In-Reply-To: {201101182014.p0IKEIIH025277-at-ns.microscopy.com} 5, 25 -- References: {201101182014.p0IKEIIH025277-at-ns.microscopy.com} 5, 25 -- Date: Tue, 18 Jan 2011 15:18:33 -0500 5, 25 -- To: Microscopy-at-microscopy.com 5, 25 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 25 -- Subject: Re: [Microscopy] Santovac 5? I need a few drops... 5, 25 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 5, 25 -- X-OriginalArrivalTime: 18 Jan 2011 20:18:35.0187 (UTC) FILETIME=[E246A830:01CBB74C] 5, 25 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 25 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 5, 25 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 25 -- X-CanItPRO-Stream: default 5, 25 -- X-Canit-Stats-ID: 02DV8izXf - d86f5723cb91 - 20110118 5, 25 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
In 1963, ten years after the papers were publlished, I joined the Lab of Dan Moore, at Rockefeller. That lab, which had been Porter's until he moved to Harvard, was located in the basement of Smith Hall, and actually extended under 68th street. At any rate, I learned microtomy on one of the mechanical advance microtomes that were built in the machine shop --I still have it--, and there was also a thermal advance version. The thermal advancement was provided by an incandescent light bulb suspended over the cutting arm. You advanced the block by flashing the light. As you might expect, the area around the microtome was forbidden to others while it was in use. Any air currents would disturb the whole process.
Joel
On Thu, Jan 13, 2011 at 5:26 PM, {NEERAJG-at-clemson.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } So I did a little digging on who reported thermal advance in ultramicrotomes first (not which commercial instruments came first) the original paper by Keith Porter and J. Blum published in 1953, A study in Microtomy for electron Microscopy, The Anatomical Record Volume 117, Issue 4, pages 685-709, December 1953, in it Porter and Blum describe two new mechanisms, one is a thermal expansion and the other is mechanical advancement (I have a PDF of this paper if anyone is interested). Here is an excerpt from the Summary of this paper } } 'Two new microtomes capable of cutting serial sections as thin as 25-50 mu are described. All moving parts in these instruments are supported in unlubricated pivots and the specimen is taken past the cutting edge only on the cutting stroke. These two features more than any others seem responsible for the successful performance of these microtomes. In one instrument, the prototype, the block is advanced to the knife by thermal expansion. In the other, the advancement is controlled mechanically.' } } Interestingly enough, Fritiof Sjostrand's paper, A new microtome for ultrathin sectioning for high resolution microscopy, 1953 Experientia 9:114-121 (note the paper also published in 1953) says 'The microtome reported on in this paper represents a further development of the microtome designed by Porter' (http://www.springerlink.com/content/y3267k5456j11117/). } } Also found an interesting book, Picture Control: The Electron Microscope and the Transformation of Biology in America by Nicolas Rasmussen, has some interesting history on this matter (see preview, http://books.google.com/books?id=rwC5QiqLS44C&pg=PA127&lpg=PA127&dq=Sjostrand+a+new+ultramicrotome&source=bl&ots=HCTXlObRJ-&sig=G46YfqxY8lrFtBptZDgmxIDbxuA&hl=en&ei=cHAvTfLRBsKSgQfMo-xa&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCwQ6AEwAg#v=onepage&q&f=false } } } Pretty Interesting! } } Best, } } Neeraj. } } } Neeraj V. Gohad, Ph.D. } Postdoctoral Fellow } Okeanos Research Group } Department of Biological Sciences } 132 Long Hall } Clemson University } Clemson,SC-29634 } Phone: 864-656-3597 } Fax: 864-656-0435 } } Website: http://www.clemson.edu/okeanos } } } } } } } } } } } } X-from: Paul Hazelton [mailto:hazeltn-at-cc.umanitoba.ca] } Sent: Wednesday, January 12, 2011 4:09 PM } To: Neeraj Gohad } Subject: Re: [Microscopy] Ernst Ruska } } Neeraj et al } } To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand. } } Regards the Nobel Prize. Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment. } } Ernst Ruska remained in Germany during WWII, and worked with the German war effort. Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb. Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable. This was the story to me in 1969, when I first started doing EM. Ruska was, of course still alive, but as told, would not be a candidate for the prize. } } Over the ensuing years Ruska sort of got forgotten. Then came the development of Scanning Tunneling EM. In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized. Whatever his personal feelings may have been, his speech was gracious, and only said: } } "Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech, December 10, 1986) } } Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved. } } Politics in the award of the Prize in all fields has been a long tradition. It still carries on. Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition. And that is a heck of a statement for someone considered to be politically to the left of centre. } } Paul Hazelton } } -- } } Paul R. Hazelton, PhD } Gastroenteric Diseases Study Group } University of Manitoba } Department of Medical Microbiology } 511 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 3J7 } e-mail: paul_hazelton-at-umanitoba.ca } Phone: 204-789-3313 (w) } 204-489-6924 (h) } Cell: 204-781-6982 } Fax: 204-789-3926 } } } ==============================Original Headers============================== } 35, 38 -- From NEERAJG-at-clemson.edu Thu Jan 13 16:18:28 2011 } 35, 38 -- Received: from mailclient3.clemson.edu (mailclient3.clemson.edu [130.127.237.182]) } 35, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0DMIShU007551 } 35, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 16:18:28 -0600 } 35, 38 -- Received: from mailclient3.clemson.edu (localhost.clemson.edu [127.0.0.1]) } 35, 38 -- by mailclient3.clemson.edu (8.13.8/8.13.8) with ESMTP id p0DMIR13007594 } 35, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:18:27 -0500 } 35, 38 -- Received: from frontex-2.CAMPUS.CU.CLEMSON.EDU (frontex-2.clemson.edu [130.127.235.60]) } 35, 38 -- by mailclient3.clemson.edu (8.13.8/8.13.8) with ESMTP id p0DMIQut007585 } 35, 38 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) } 35, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Jan 2011 17:18:26 -0500 } 35, 38 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by } 35, 38 -- frontex-2.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb3c::827f:eb3c]) with mapi; Thu, } 35, 38 -- 13 Jan 2011 17:18:26 -0500 } 35, 38 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} } 35, 38 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 35, 38 -- Date: Thu, 13 Jan 2011 17:18:26 -0500 } 35, 38 -- Subject: RE: [Microscopy] Ernst Ruska } 35, 38 -- Thread-Topic: [Microscopy] Ernst Ruska } 35, 38 -- Thread-Index: AcuynOdoI32UWIhFRl6VmRIQcrDJMAAzMIHw } 35, 38 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F9938625FE3-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} } 35, 38 -- References: {201101121903.p0CJ3jkv009165-at-ns.microscopy.com} } 35, 38 -- {4D2E1851.4040202-at-cc.umanitoba.ca} } 35, 38 -- In-Reply-To: {4D2E1851.4040202-at-cc.umanitoba.ca} } 35, 38 -- Accept-Language: en-US } 35, 38 -- Content-Language: en-US } 35, 38 -- X-MS-Has-Attach: } 35, 38 -- X-MS-TNEF-Correlator: } 35, 38 -- acceptlanguage: en-US } 35, 38 -- Content-Type: text/plain; charset="iso-8859-1" } 35, 38 -- MIME-Version: 1.0 } 35, 38 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 } 35, 38 -- definitions=2011-01-13_09:2011-01-13,2011-01-13,1970-01-01 signatures=0 } 35, 38 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 } 35, 38 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx } 35, 38 -- engine=5.0.0-1012030000 definitions=main-1101130148 } 35, 38 -- Content-Transfer-Encoding: 8bit } 35, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0DMIShU007551 } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 7, 35 -- From joelsheffield-at-gmail.com Tue Jan 18 16:20:08 2011 7, 35 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0IMK7qv031430 7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 16:20:08 -0600 7, 35 -- Received: by wyj26 with SMTP id 26so162455wyj.0 7, 35 -- for {microscopy-at-microscopy.com} ; Tue, 18 Jan 2011 14:20:07 -0800 (PST) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 7, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 7, 35 -- bh=4Ux6VAoDeCHPyVoM9Y72oMaxs+yPFsDcnJZCxedVFx8=; 7, 35 -- b=q0Djo0tfKkXTsB1HyRqTSAkxttUaLtYeElyAP1334TWduBfvweNRRnAoEXAc1bWwPQ 7, 35 -- oyurd1mBd7pXVjbFtWF3Wn131uh7pwDJzRONynxBM7pAx9NKv2UgcRE5mFZ+4xrzFC5i 7, 35 -- wy7mWRKeuK1PCS2ntLRUi9ry7/HxcvKYJQ1No= 7, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 7, 35 -- :content-type:content-transfer-encoding; 7, 35 -- b=HlXhCjMYmz5BLqOAq5cXYutZVGco4ITzK83i2yKBFW7eKNhJivkmLkdwcY71FtwQZC 7, 35 -- D60fyp89pBLFaIJyCy4Di48Gl14illOKP3uCBvCQXrAjektUFEmQG9VGdPWOPnKJukbR 7, 35 -- GV/LXZ3SzbXaEsBK40RwEzLLOCcpxSK9vBOlI= 7, 35 -- MIME-Version: 1.0 7, 35 -- Received: by 10.227.182.68 with SMTP id cb4mr6280208wbb.218.1295389206945; 7, 35 -- Tue, 18 Jan 2011 14:20:06 -0800 (PST) 7, 35 -- Received: by 10.227.143.213 with HTTP; Tue, 18 Jan 2011 14:20:06 -0800 (PST) 7, 35 -- In-Reply-To: {201101132226.p0DMQEek018977-at-ns.microscopy.com} 7, 35 -- References: {201101132226.p0DMQEek018977-at-ns.microscopy.com} 7, 35 -- Date: Tue, 18 Jan 2011 17:20:06 -0500 7, 35 -- Message-ID: {AANLkTinRDV6AB=4g=G4YztWiMVtXapMc=fzVYz82QPFa-at-mail.gmail.com} 7, 35 -- Subject: Re: [Microscopy] RE: Ernst Ruska 7, 35 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 7, 35 -- To: NEERAJG-at-clemson.edu, microscopy-at-microscopy.com 7, 35 -- Content-Type: text/plain; charset=ISO-8859-1 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0IMK7qv031430 ==============================End of - Headers==============================
Thanks to all who replied with offers for some Santovac 5. I possibly have a very local source, plus some other possibilities, so others who were thinking of helping out are off the hook. Promised sacrifices to the EM gods have already been offered. Just waiting now for the smoke to clear...
As usual, the Listserv is an invaluable go-to source for all sorts of goofy problems, esp. encountered in the wilds of the Great White North.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Directions: That useless little piece of paper, containing incomprehensible language, usually written in Spanish and Japanese, that one refers to, only after botching the construction of the over-priced and (also usually) useless item that one found so necessary to own, only that very morning.
==============================Original Headers============================== 10, 19 -- From jehrman-at-mta.ca Wed Jan 19 07:17:06 2011 10, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.114]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDH5SO015384 10, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:17:06 -0600 10, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:56716) 10, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 10, 19 -- (Exim 4.71) 10, 19 -- (envelope-from {jehrman-at-mta.ca} ) 10, 19 -- id 1PfXuJ-00014B-BJ 10, 19 -- for Microscopy-at-microscopy.com; Wed, 19 Jan 2011 09:17:03 -0400 10, 19 -- Message-ID: {4D36E44D.8010700-at-mta.ca} 10, 19 -- Date: Wed, 19 Jan 2011 09:17:01 -0400 10, 19 -- From: James Ehrman {jehrman-at-mta.ca} 10, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 19 -- MIME-Version: 1.0 10, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 10, 19 -- Subject: Thanks! Re: Santovac 5? I need a few drops... 10, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I’ve been thinking about EDS line scans and I’m attempting to maximize the data I collect.
I run at 20 KV on iron samples so my electron interaction range is between 1.5 to 2.3um (Berthe or K-O range calculations). According to data provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at 700x. So right away I see if each image pixel represents a data point, I’m sampling about 2 pixel volumes per data point. Looking for a sudden and sharp change in element concentration seem difficult, but wait there’s more. Let us introduce another plot complication.
My customers don’t want to scan the entire image, tooo much data…
So I scan a smaller section of image described above and instruct the computer to take 512 data points along a line that might only be 400 (visual estimate from screen) image pixels long.
So… Should I reduce the image resolution so that each image pixel will be one data point and scan the entire image? Would it be better to lower the magnification so the image pixels are larger (say 1.2um ). I could go to 100x and 512 horizontal image pixel so each image pixel is 2.3um?
I’m looking for slight changes in element concentration at grain boundaries and precipitates and of course I want to produce the most meaningful data possible. I’m running at conditions that suggest my electron interactive volume is larger than my spatial resolution as to produce “continuous†data.
Or am I just over thinking the process?
Any suggestion or comments would be welcome
Frank Lincoln Electric Company -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 17 -- From frank_karl-at-lincolnelectric.com Wed Jan 19 07:37:23 2011 11, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDbNoO031503 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:37:23 -0600 11, 17 -- Subject: EDS line scans and time on my hands 11, 17 -- To: Microscopy-at-microscopy.com 11, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 17 -- Message-ID: {OF4A811DE5.D47D1408-ON8525781D.004A569D-8525781D.004AD372-at-lincolnelectric.com} 11, 17 -- From: Frank_Karl-at-lincolnelectric.com 11, 17 -- Date: Wed, 19 Jan 2011 08:38:03 -0500 11, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 11, 17 -- 01/19/2011 08:37:18 AM 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; 11, 17 -- charset="UTF-8" 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0JDbNoO031503 ==============================End of - Headers==============================
Join the LinkedIn Group at http://www.linkedin.com/groupRegistration?gid=3733425
And check out The Cell: An Image Library at www.cellimagelibrary.org.
David
David Orloff Manager, Image Library The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 T: 301-347-9300/Direct: 301-347-9305 F: 301-347-9310 E-mail: dorloff-at-ascb.org Web site: www.ascb.org The Cell: An Image LibraryT: www.cellimagelibrary.org
==============================Original Headers============================== 9, 25 -- From DOrloff-at-ascb.org Wed Jan 19 08:25:59 2011 9, 25 -- Received: from smtp.ascb.org (coalitionforlifesciences.org [173.13.233.102] (may be forged)) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JEPxGk016078 9, 25 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 08:25:59 -0600 9, 25 -- Received: from ASCB2.ascb.local ([::1]) by ASCB2.ascb.local ([::1]) with mapi; 9, 25 -- Wed, 19 Jan 2011 09:25:57 -0500 9, 25 -- From: David Orloff {DOrloff-at-ascb.org} 9, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 9, 25 -- CC: David Orloff {DOrloff-at-ascb.org} 9, 25 -- Date: Wed, 19 Jan 2011 09:25:57 -0500 9, 25 -- Subject: Cell Microscopy 9, 25 -- Thread-Topic: Cell Microscopy 9, 25 -- Thread-Index: Acu35EQpVdvSPTRcRiqphmn0f0HxFQAAGyHw 9, 25 -- Message-ID: {8D9C2A7731877D45B1691E78451EC3BE4C6BE08D-at-ASCB2.ascb.local} 9, 25 -- References: {8D9C2A7731877D45B1691E78451EC3BE4C6BE08C-at-ASCB2.ascb.local} 9, 25 -- In-Reply-To: {8D9C2A7731877D45B1691E78451EC3BE4C6BE08C-at-ASCB2.ascb.local} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- acceptlanguage: en-US 9, 25 -- Content-Type: text/plain; charset="iso-8859-1" 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JEPxGk016078 ==============================End of - Headers==============================
For those who may find themselves in my predicament, Bart has small quantities of Santovac 5 available:
Jim,
I have Santovac 5 in small glass vials. 30 drops for $10.
You might try just brushing out the lead screws with acetone and a paper towel guard to collect the spray. That will disperse any residual coagulated Santovac 5.
Though I've been on this list for almost its entire life, I can no longer post to the group since I can't get through the spam filter.
You might consider posting this e-mail to the group.
Bart Cannon Cannon Microprobe 1041 NE 100th Seattle, WA 98125 206 522 9233
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Directions: That useless little piece of paper, containing incomprehensible language, usually written in Spanish and Japanese, that one refers to, only after botching the construction of the over-priced and (also usually) useless item that one found so necessary to own, only that very morning.
==============================Original Headers============================== 13, 19 -- From jehrman-at-mta.ca Wed Jan 19 08:33:21 2011 13, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.114]) 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JEXK0o028862 13, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 08:33:21 -0600 13, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:56984) 13, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 13, 19 -- (Exim 4.71) 13, 19 -- (envelope-from {jehrman-at-mta.ca} ) 13, 19 -- id 1PfZ68-0002je-LA 13, 19 -- for Microscopy-at-microscopy.com; Wed, 19 Jan 2011 10:33:20 -0400 13, 19 -- Message-ID: {4D36F62E.3040106-at-mta.ca} 13, 19 -- Date: Wed, 19 Jan 2011 10:33:18 -0400 13, 19 -- From: James Ehrman {jehrman-at-mta.ca} 13, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 13, 19 -- MIME-Version: 1.0 13, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 13, 19 -- Subject: source of small amounts of Santovac 5 13, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are trying to revive a Fuji Pitctrography 3000. We have the SCSI to USB adapters but the pc we have still does not see that the printer is there. We are using a pc with the Vista opreating system (don't start with the comments about that!) and the Fuji uses Photoshop plug-ins.
Any suggestions as to what to try next to get this printer up and runing?
Thanks in advance.
Paula
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#M251 Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 7, 33 -- From patpxs-at-gmail.com Wed Jan 19 11:07:36 2011 7, 33 -- Received: from mail-px0-f169.google.com (mail-px0-f169.google.com [209.85.212.169]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JH7aga021466 7, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 11:07:36 -0600 7, 33 -- Received: by pxi12 with SMTP id 12so261253pxi.0 7, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 09:07:35 -0800 (PST) 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 33 -- d=gmail.com; s=gamma; 7, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 7, 33 -- :content-type:content-transfer-encoding; 7, 33 -- bh=3/OjsPuFR29OGrQhvHgsM7IY/iVkuv9HWZjelQ5PVMk=; 7, 33 -- b=vkM9a1Iq07A3dwNe+ZhdWk7lbyi6iAKGVzzLBSEHijXr1goqQgi9KT6CYVxTFDNTTv 7, 33 -- G8JZahYAx5ATcMxhAx+FfSsr56GKlOn8coV5kk27iYDTrTmYMd4Yq4EhsPHmxcXlavr/ 7, 33 -- 59evQkwZmybdjYc4Q1dIJ4rj9vZ8O8aCCn294= 7, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 33 -- d=gmail.com; s=gamma; 7, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 7, 33 -- :content-transfer-encoding; 7, 33 -- b=b5ddZv8nZUF3LbBw7t5zTX7jiOZTmMOVg9PPNsolpkXN2N0GXBwFpL2N257Slrrpps 7, 33 -- lKYyKW/Q7fOowq295rBP0kPs/JNB+iprMg+9Ynk2QYWPxu0CXwEtDYudo6piSTV7NqVl 7, 33 -- QrW7oTdPSnOj6wvwG+6NTfLkpiKp07SMxJNYA= 7, 33 -- MIME-Version: 1.0 7, 33 -- Received: by 10.42.172.130 with SMTP id n2mr1164409icz.133.1295456855411; Wed, 7, 33 -- 19 Jan 2011 09:07:35 -0800 (PST) 7, 33 -- Received: by 10.42.219.68 with HTTP; Wed, 19 Jan 2011 09:07:35 -0800 (PST) 7, 33 -- Date: Wed, 19 Jan 2011 12:07:35 -0500 7, 33 -- Message-ID: {AANLkTikeTzxtk6s-pi+X4r7+xQ6zU=rQf37J3iR2X8rt-at-mail.gmail.com} 7, 33 -- Subject: Fuji Pictrography 3000 7, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 7, 33 -- To: Microscopy-at-microscopy.com 7, 33 -- Content-Type: text/plain; charset=ISO-8859-1 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JH7aga021466 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ewestbrook-at-vsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] How do I remove front panels on Hitachi TM-1000
Message: Hello All, I need to remove the front panels on the Hitachi TM-1000 Tabletop SEM. Has anyone done this? If so, please respond. This is not easy! I want to have the turbo molecular pump serviced. Thanks! Winnie Westbrook, M.Ed. Electron Microscopy Research Lab Virginia State University Petersburg, VA 23806 804-524-5659
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both anthonyribaudo3-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: anthonyribaudo3-at-gmail.com Name: Anthony Ribaudo
Organization: TRI Princeton
Title-Subject: [Filtered] SEM imaging of wax
Message: Can anyone suggest guidelines for imaging a paraffin wax coating on a metal or polymeric substrate via FESEM. The plan is to detect the wax layer that is thought to be several hundred nanometers thick? Are there any waxes that would be more stable to image under the electron beam than paraffin wax?
A postdoc/research associate position supported by a newly renewed 5-year NIH grant is available immediately in the Department of Biochemistry, NYU School of Medicine. The research of the position focuses on structural studies, using cryo-electron microscopy and electron tomography, of uro-epithelial apical membrane and its receptor function for uropathogenic E. coli (UPEC) in urinary tract infection (UTI) (see lab webpage: http://kong.med.nyu.edu). Experience in electron microscopy and image processing is desirable. Interested candidates should send CV and reference information to xiangpeng.kong-at-med.nyu.edu.
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 5, 32 -- From Fengxia.Liang-at-med.nyu.edu Wed Jan 19 14:33:35 2011 5, 32 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JKXZf5014480 5, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:35 -0600 5, 32 -- X-IronPort-Anti-Spam-Filtered: true 5, 32 -- X-IronPort-Anti-Spam-Result: ApsEAEvZNk0KkyXk/2dsb2JhbAClO7cpiDWFUASEbw 5, 32 -- X-IronPort-AV: E=Sophos;i="4.60,345,1291611600"; 5, 32 -- d="scan'208";a="112262987" 5, 32 -- Received: from msgwsdcpht62.nyumc.org ([10.147.37.228]) 5, 32 -- by mail4.nyumc.org with SMTP; 19 Jan 2011 15:33:34 -0500 5, 32 -- Received: from MSGWSDCPMB05.nyumc.org ([10.147.54.52]) by 5, 32 -- MSGWSDCPHT62.nyumc.org ([10.147.37.228]) with mapi; Wed, 19 Jan 2011 15:33:35 5, 32 -- -0500 5, 32 -- From: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 5, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 32 -- Date: Wed, 19 Jan 2011 15:33:33 -0500 5, 32 -- Subject: Postdoc/research associate position available at NYU School 5, 32 -- ofMedicine 5, 32 -- Thread-Topic: Postdoc/research associate position available at NYU School 5, 32 -- ofMedicine 5, 32 -- Thread-Index: Acu4GCPT+mxRv30pMEaqNBbF070AyQ== 5, 32 -- Message-ID: {C95CB4CD.65DE%Fengxia.Liang-at-med.nyu.edu} 5, 32 -- Accept-Language: en-US 5, 32 -- Content-Language: en-US 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- user-agent: Microsoft-Entourage/13.4.0.100208 5, 32 -- acceptlanguage: en-US 5, 32 -- Content-Type: text/plain; charset="us-ascii" 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JKXZf5014480 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Dept of Materials, Loughborough University, UK
Title-Subject: [Filtered] TEM diffraction 3nm precipitates
Message: Greetings listers,
Iím studying crystal structure of some carbides/nitrides precipitates in steel. The precipitates are 2-5nm size in a C replica TEM specimen, V-, Cr-, and Nb-rich by STEM/EDX.
How can I get electron diffraction or crystal structure information of these fine precipitates?
I tried on a TecnaiF20 TEM using nanoprobe spot 7, focused on a small particle, then diffraction, most of the time I got amorphous rings of C support, no obvious diffraction discs. STEM mode did indicate some C contamination on the session. Assuming minimal C contamination, is it possible that this method can give me some sort of convergence beam electron diffraction pattern, which may help me determine the crystal structure of the precipitates?
Iím also open to other methods.
Your advice is highly appreciated.
Zhou
Dr Zhaoxia Zhou Dept of Materials Loughborough University Loughborough, UK LE11 3TU
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbarlow-at-sciences.sdsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] color Laser printers
Message: Hello All
Our general lab printer, a monochrome HP 2100, is on its last legs and needs to be replaced, preferably with a color, duplex capable printer.
We are looking at the Ricoh C430dn or the HP CP4525dn. We use the lab printer for copy images, documentation of images. student notebooks, etc.
I have had lots of experience with the HP printers, but none with the Ricoh. Can anyone give me insights into their own experiences with these two printers or the companies? Any other recommendations for a laser printer?
We will be imaging a fairly large number of norovirus samples using negative staining. This is just for sample screening to document the presence or absence of the virus.
The samples could be sent in fresh but I thought it might be better to fix them prior to shipping so that they are not a health hazard. That might also allow us to store them unfrozen for an extended period of time (months) with reduced chance of deterioration or contamination.
Does anyone have a validated protocol that they are willing to share? I used to know someone at the CDC who did TEM but no longer have that contact.
Thanks, Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 7, 28 -- From dsherman-at-purdue.edu Wed Jan 19 15:36:50 2011 7, 28 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JLanJM026947 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 15:36:50 -0600 7, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 7, 28 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0JLan3m008331 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:36:49 -0500 7, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.110]) by WPPEXHUB01F.purdue.lcl 7, 28 -- ([::1]) with mapi; Wed, 19 Jan 2011 16:36:48 -0500 7, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 7, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 28 -- Date: Wed, 19 Jan 2011 16:36:46 -0500 7, 28 -- Subject: Protocol for Norovirus TEM 7, 28 -- Thread-Topic: Protocol for Norovirus TEM 7, 28 -- Thread-Index: Acu4IPihFFWUNBgITEScuEiQwrSisg== 7, 28 -- Message-ID: {C95CC39E.B10D%dsherman-at-purdue.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- X-PMX-Version: 5.5.9.388399 7, 28 -- X-PerlMx-Virus-Scanned: Yes 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JLanJM026947 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abel.orellana-at-sydney.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abel.orellana-at-sydney.edu.au Name: Abel Orellana
Organization: University of Sydney
Title-Subject: [Filtered] University of Sydney - Career Opportunity
Message: GENERAL OFFICE ADMINISTRATOR THE AUSTRALIAN CENTRE FOR MICROSCOPY AND MICROANALYSIS (ACMM) REFERENCE NO. 2615 /0810
ï Be part of the largest Microscopy Facility of its type in Australia ï Opportunity to work on newly emerging microscopy techniques ï Remuneration package: $80,157
The University of Sydney is Australia's premier University with an outstanding global reputation for academic and research excellence, and employs over 6,800 permanent staff supporting over 46,000 students.
The Australian Centre for Microscopy and Microanalysis (ACMM) aims to provide leadership in the development of innovation and ingenuity in Australian science and engineering. The Centreís vision is to be a world-leading facility for modern microscopy and microanalysis, offering premier instruments, services and training to researchers from across Sydney and around Australia, and providing strong leadership in the national and international characterisation communities.
We are currently seeking an experienced Administrator to provide effective front desk and administrative support to the main office of the ACMM. Ideally suited to someone with an interest in science, this is an excellent opportunity to assist in the co-ordination of research administration actives within research services.
You will be responsible for the professional maintenance of the ACMM front desk, while being first point-of contact for all queries. As such it is imperative that you have excellent communication and problem solving skills. The position will see you co-ordinate and improve the ACMM administration procedures including but not limited to, arranging new user meetings; liaising with users and visitors to the Centre in terms of access; coordinating inductions for new staff and students and providing support to the maintenance of the Centreís website. Your demonstrated ability to adapt to change and willingness to undertake new tasks as the need arises will be essential.
We are looking for a committed individual who will thrive in a busy and changing environment. To succeed, you will have previous experience working in a busy office environment, existing high level IT skills including creation of spreadsheets, experience drafting and editing reports along with experience producing high level meeting minutes. You must also have strong development and project management skills and the ability to communicate with a diverse range of individuals. Previous experience in a Tertiary environment will be highly regarded.
The position is continuing subject to the completion of a satisfactory probation period for new appointees. Membership of a University approved superannuation scheme is a condition of employment for new appointees.
Remuneration package: a competitive remuneration package is available of $80,157 (consisting of a base salary $67,734, leave loading and up to 17% employerís contribution to superannuation).
All applications must be submitted via The University of Sydney careers website. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
CLOSING DATE: 31 January 2011
The University is an Equal Opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged.
The University reserves the right not to proceed with any appointment.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abel.orellana-at-sydney.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abel.orellana-at-sydney.edu.au Name: Abel Orellana
Organization: University of Sydney
Title-Subject: [Filtered] University of Sydney - Career Opportunity
Message: SCANNING ELECTRON MICROSCOPY SPECIALIST THE AUSTRALIAN CENTRE FOR MICROSCOPY AND MICROANALYSIS (ACMM) REFERENCE NO. 4273 /1210
ï Be part of the largest Microscopy Facility of its type in Australia ï Opportunity to work on newly emerging microscopy techniques ï Remuneration package: $80,157
The University of Sydney is Australia's premier University with an outstanding global reputation for academic and research excellence, and employs over 6,800 permanent staff supporting over 46,000 students.
The Australian Centre for Microscopy and Microanalysis (ACMM) aims to provide leadership in the development of innovation and ingenuity in Australian science and engineering. The Centreís vision is to be a world-leading facility for modern microscopy and microanalysis, offering premier instruments, services and training to researchers from across Sydney and around Australia, and providing strong leadership in the national and international characterisation communities.
As the Scanning Electron Microscopy Specialist you will provide instruction, training and support to users of the Centre's analytical scanning electron microscope facilities including primarily the needs of users in specimen preparation, microscopy, microanalysis, image/data analysis and interpretation. You will have demonstrated expertise and skills in specimen preparation techniques relevant to scanning electron microscopy (SEM) and associated techniques and skills in one or more of the following: focussed ion beam milling (FIB) techniques; environmental SEM (ESEM), cryo-SEM imaging; In-Situ SEM instrumentation; Electron Backscattered Diffraction (EBSD); quantitative image analysis; X-ray microanalysis, X-ray mapping; Mineral phase mapping
To succeed, you will possess an Honours degree in science or engineering whereas a doctoral degree with a high SEM component will be highly advantageous. You will be a self motivated individual with exceptional scientific communication skills and a demonstrated ability to relate to students, users, academic and researchers at all levels. Experience in running and working in a multi-user laboratory and experience with the instruction of SEM techniques is desirable.
The position is full-time, fixed term for three years subject to the completion of a satisfactory probation period for new appointees with the possibility of further offer of employment. Membership of a University approved superannuation scheme is a condition of employment for new appointees. Visa sponsorship and relocation assistance may be offered to suitable overseas applicants. A wide range of employment benefits are also available, including Living Away From Home Allowance (LAFHA).
Remuneration package: a competitive remuneration package is available of $80,157 (consisting of a base salary $67,734, leave loading and up to 17% employerís contribution to superannuation).
All applications must be submitted via The University of Sydney careers website. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
CLOSING DATE: 31 January 2011
The University is an Equal Opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged.
The University reserves the right not to proceed with any appointment.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both a.o.orellana as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: a.o.orellana Name: Abel Orellana
Organization: University of Sydney
Title-Subject: [Filtered] University of Sydney - Career Opportunity
Message: MULTIMEDIA COMMUNICATIONS OFFICER AUSTRALIAN MICROSCOPY & MICROSCOPY RESEARCH FACILITY (AMMRF) REFERENCE NO. 4239 /1210
ï Collaborate with AMMRF partners ï Opportunity to work with a variety of Media Communications ï Remuneration package: $80,157 ï The University of Sydney is Australia's premier University with an outstanding global reputation for academic and research excellence, and employs over 6,800 permanent staff supporting over 46,000 students.
The ACMM provides leadership in the development and application of advanced microscopy and microanalysis techniques that support innovation in Australian science and engineering. The centre incorporates a substantial research portfolio, is a node of the ARC Centre if Excellence for Design in Light Metals and is the headquarters for the AMMRF, The AMMRF is Australiaís national research facility for the characterisation of materials through macro, meso, nano and atomic length scales by means of advanced microscopy and microanalysis. The facility is a joint venture of eight universities, which forms a grid of microscopy facilities with strategic links to six other laboratories throughout Australia.
As the Multimedia Communications Officer you will collaborate with AMMRF partners as well as teams within the University of Sydney, to facilitate and produce high-quality communications and promotional outcomes. This will include the creation, design and production of relevant materials utilising a variety of media such as written documents, presentations and the AMMRF and ACMM websites. You will also assist in the organisation and management of major events and brand implementation for the AMMRF and the ACMM.
To be successful you must have previous experience in the production of high quality print and digital communications as well as the ability to draft, edit and analyse print for reports, publications and presentations. Your experience and knowledge working with web authoring, web design and graphic design software as well your exceptional working knowledge of Adobe Creative Suite Premium and Microsoft Office is essential, as is the ability to build and maintain effective working relationships. Previous experience working in a scientific organisation will be highly regarded.
The position is for 2.5 years, fixed term subject to the completion of a satisfactory probation period for new appointees with the possibility of further offer of employment. Membership of a University approved superannuation scheme is a condition of employment for new appointees.
Remuneration package: a competitive remuneration package is available of $80,157 (consisting of a base salary $67,734 leave loading and up to 17% employerís contribution to superannuation).
All applications must be submitted via The University of Sydney careers website. Visit sydney.edu.au/positions and search by the reference number for more information and to apply.
CLOSING DATE: 31 January 2011
The University is an Equal Opportunity employer committed to equity, diversity and social inclusion. Applications from equity target groups and women are encouraged.
Appointment is on merit; as women are under-represented at this employment level suitably qualified women are encouraged to apply.
The University reserves the right not to proceed with any appointment.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin Foley
Organization: UAB
Title-Subject: [Filtered] Environmental SEM for Biological Work
Message: Is there heavy use of ESEM for biological SEM? What is commonly done on biological ESEM samples? We're looking at purchasing an environmental SEM and the sales rep. tells us we will need a Peltier stage to cool the sample to look at wet samples. Unfortunately, the maximum sample size for the Peltier stage is 3 mm across. This seems tiny to me for an SEM. Are there many biological ESEM applications that don't require the sample to be wet?
Thanks,
Robin Foley (who spends most of her SEM time looking at metals, ceramics and polymers!)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both David.Llewellyn-at-anu.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: David.Llewellyn-at-anu.edu.au Name: David Llewellyn
Message: Am after some information re the Tenupol-2 and associated Polypower Power Supply best of all would be off-line with somebody who has one of these, thanks, David.
*Post-Doctoral Research Associate Position in Aberration-Corrected STEM*
* *
A post-doctoral research associate position is available in the Nanoscale Physics Group at the University of Illinois at Chicago to work on the application of atomic-resolution Z-contrast and annular bright-field imaging, as well as electron energy-loss spectroscopy (EELS). The successful candidate will work with our new JEOL ARM200CF, an aberration-corrected cold-field emission STEM with sub-Å and sub-eV resolution, equipped with several in-situ holders.
Candidates should have a Ph.D. in Physics, Materials Science or related disciplines. The position requires extensive experience in transmission electron microscopy and electron energy-loss spectroscopy. Experience working with aberration-corrected scanning transmission electron microscopy is also preferred.
This postdoctoral position is available immediately, will be renewable on an annual basis, and is anticipated for at least two years. The position is open until it is filled.
Interested candidates can apply by email by sending a cover letter, CV with a complete list of publications, and contact information of 3 professional references to:
Professor Robert F Klie
Department of Physics
University of Illinois at Chicago
845 W Taylor Street, M/C 273
Chicago, IL 60607
email: rfklie-at-uic.edu
-- Robert F. Klie, PhD Assistant Professor University of Illinois at Chicago Department of Physics Chicago, IL 60607 Tel: 312-996-6064 Fax: 312-996-9016
Editor Journal of Undergraduate Research Website {http://jur.phy.uic.edu}
President Midwest Microscopy and Microanalysis Society (M^3 S) Website {http://midwestmicroscopy.org}
==============================Original Headers============================== 15, 18 -- From rfklie-at-uic.edu Wed Jan 19 17:18:47 2011 15, 18 -- Received: from mail-4.cc.uic.edu (mail-4.cc.uic.edu [128.248.155.184]) 15, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JNIlo0025624 15, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 17:18:47 -0600 15, 18 -- Received: from [131.193.192.91] (klielaptop.phy.uic.edu [131.193.192.91]) 15, 18 -- (authenticated bits=0) 15, 18 -- by mail-4.cc.uic.edu (8.14.4/8.14.4) with ESMTP id p0JNIlFP021499 15, 18 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 15, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 17:18:47 -0600 15, 18 -- Message-ID: {4D377152.4010000-at-uic.edu} 15, 18 -- Date: Wed, 19 Jan 2011 17:18:42 -0600 15, 18 -- From: Robert Klie {rfklie-at-uic.edu} 15, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 15, 18 -- MIME-Version: 1.0 15, 18 -- To: Microscopy-at-microscopy.com 15, 18 -- Subject: STEM post-doc position at UIC 15, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
It is certainly clear that there are many interesting stories --some urban legends, and some insights into the minds of those that founded the discipline of thin section electron microscopy. During the mid '70's, I taught an EM course at Rutgers, Camden. In the basement, along with an RCA EMU-2 (!) was a thermal advance microtome that I was told was developed by Sjostrand. This thing consisted of a disk-like plate, about 4-5 inches in diameter, with an eccentrically mounted arm that stuck out of the plate. The arm had a chuck for a tissue sample, and a heating coil. The plate itself was mounted in a frame and lubricated with oil --the plate acted like a large oil bearing. The plate was linked to a motor on the wall by a long V belt (to minimize vibration), and the entire plate rotated, bringing the block past the knife on each rotation. The sections that resulted tended to be arc shaped. We got it working, but it was a terrifying thing to see. I wonder if anyone in this group remembers this type of machine --assuming that my description is comprehensible.
The other microtome that I used, and really admire, was the Huxley microtome, originally sold by Cambridge, and ultimately marketed by LKB. Although it used a mechanical advance, all of the movements of the cutting arm were made by bending sheets of spring steel, so that there were no surfaces that moved against each other. Moreover, the cutting stroke was controlled by an oil-filled damper, to minimize chatter. --a remarkable and stable machine.
Now, the urban legend. I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections. How many strokes, in which direction, which side of the blade, etc. During the discussion, a bottle of Coke was knocked off the table and broke into many pieces. The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone?
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 5, 30 -- From joelsheffield-at-gmail.com Wed Jan 19 23:47:26 2011 5, 30 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0K5lQgB018304 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 23:47:26 -0600 5, 30 -- Received: by wwb17 with SMTP id 17so256663wwb.18 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 21:47:25 -0800 (PST) 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 30 -- d=gmail.com; s=gamma; 5, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 5, 30 -- :content-type; 5, 30 -- bh=kPUiuV1UxIO0H1pRVf1DSBLH7XQoy5aO2bN7+G+szxM=; 5, 30 -- b=UXJCg0QqUwPbZUaTOkLx3i+Tq22ZLycH3163aMjr/YaOTUlNXA+V3Uah88ZvAbPim3 5, 30 -- F7lWxUBBph/7Gqa/wiZDTEikXy4U+eCZ5a2FDRoLqH2T23rZ0bmrhTcekQ2FpBKKwEtC 5, 30 -- cMlEC0ztRmc2ZtJPpd93Zra4IQSLR9zEbBxYE= 5, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 30 -- d=gmail.com; s=gamma; 5, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 5, 30 -- b=rwKrTeNXOA45KaZiFgYiUR8hEDH/WD4BYLEe+mTGsnjAUl+zABKfV824sTmyEd7EwU 5, 30 -- FeXrP78Ws/O0V/Tvzil7+UlEWx3u0y+u9UG3QRXhbJtGDNRwFUpTop9td+FSdKQ8ZDmD 5, 30 -- a/L+pGrqTp0BPxBRSJFCfG/idbLcFzFb4b6fg= 5, 30 -- MIME-Version: 1.0 5, 30 -- Received: by 10.227.155.66 with SMTP id r2mr1805845wbw.73.1295502444180; Wed, 5, 30 -- 19 Jan 2011 21:47:24 -0800 (PST) 5, 30 -- Received: by 10.227.143.213 with HTTP; Wed, 19 Jan 2011 21:47:24 -0800 (PST) 5, 30 -- Date: Thu, 20 Jan 2011 00:47:24 -0500 5, 30 -- Message-ID: {AANLkTikoNq0MQhwanuX3FQwAJFQB7tscWZmu_MivkGTi-at-mail.gmail.com} 5, 30 -- Subject: Microtome History - was "Ernst Ruska" 5, 30 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 5, 30 -- To: microscopy-at-microscopy.com 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
This urban legend differs slightly according to the origin of the narrator:
An american talks about a bottle of coke. A french talks about a bottle of ricard A belgian talks about a flask of beer An italian talks about a bottle of wine A german talks about a bottle of schnaps A russian talks about a bottle of vodka. A japanese talks about a bottle of sake
Actually it would be interesting to test all of them to see which one cuts better. Now you just need convincing words for your grant....
Stephane
----- Original Message ---- X-from: "joelsheffield-at-gmail.com" {joelsheffield-at-gmail.com} To: nizets2-at-yahoo.com Sent: Thu, January 20, 2011 6:51:55 AM
It is certainly clear that there are many interesting stories --some urban legends, and some insights into the minds of those that founded the discipline of thin section electron microscopy. During the mid '70's, I taught an EM course at Rutgers, Camden. In the basement, along with an RCA EMU-2 (!) was a thermal advance microtome that I was told was developed by Sjostrand. This thing consisted of a disk-like plate, about 4-5 inches in diameter, with an eccentrically mounted arm that stuck out of the plate. The arm had a chuck for a tissue sample, and a heating coil. The plate itself was mounted in a frame and lubricated with oil --the plate acted like a large oil bearing. The plate was linked to a motor on the wall by a long V belt (to minimize vibration), and the entire plate rotated, bringing the block past the knife on each rotation. The sections that resulted tended to be arc shaped. We got it working, but it was a terrifying thing to see. I wonder if anyone in this group remembers this type of machine --assuming that my description is comprehensible.
The other microtome that I used, and really admire, was the Huxley microtome, originally sold by Cambridge, and ultimately marketed by LKB. Although it used a mechanical advance, all of the movements of the cutting arm were made by bending sheets of spring steel, so that there were no surfaces that moved against each other. Moreover, the cutting stroke was controlled by an oil-filled damper, to minimize chatter. --a remarkable and stable machine.
Now, the urban legend. I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections. How many strokes, in which direction, which side of the blade, etc. During the discussion, a bottle of Coke was knocked off the table and broke into many pieces. The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone?
Joel
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 5, 30 -- From joelsheffield-at-gmail.com Wed Jan 19 23:47:26 2011 5, 30 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0K5lQgB018304 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 23:47:26 -0600 5, 30 -- Received: by wwb17 with SMTP id 17so256663wwb.18 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 21:47:25 -0800 (PST) 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 30 -- d=gmail.com; s=gamma; 5, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 5, 30 -- :content-type; 5, 30 -- bh=kPUiuV1UxIO0H1pRVf1DSBLH7XQoy5aO2bN7+G+szxM=; 5, 30 -- b=UXJCg0QqUwPbZUaTOkLx3i+Tq22ZLycH3163aMjr/YaOTUlNXA+V3Uah88ZvAbPim3 5, 30 -- F7lWxUBBph/7Gqa/wiZDTEikXy4U+eCZ5a2FDRoLqH2T23rZ0bmrhTcekQ2FpBKKwEtC 5, 30 -- cMlEC0ztRmc2ZtJPpd93Zra4IQSLR9zEbBxYE= 5, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 30 -- d=gmail.com; s=gamma; 5, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 5, 30 -- b=rwKrTeNXOA45KaZiFgYiUR8hEDH/WD4BYLEe+mTGsnjAUl+zABKfV824sTmyEd7EwU 5, 30 -- FeXrP78Ws/O0V/Tvzil7+UlEWx3u0y+u9UG3QRXhbJtGDNRwFUpTop9td+FSdKQ8ZDmD 5, 30 -- a/L+pGrqTp0BPxBRSJFCfG/idbLcFzFb4b6fg= 5, 30 -- MIME-Version: 1.0 5, 30 -- Received: by 10.227.155.66 with SMTP id r2mr1805845wbw.73.1295502444180; Wed, 5, 30 -- 19 Jan 2011 21:47:24 -0800 (PST) 5, 30 -- Received: by 10.227.143.213 with HTTP; Wed, 19 Jan 2011 21:47:24 -0800 (PST) 5, 30 -- Date: Thu, 20 Jan 2011 00:47:24 -0500 5, 30 -- Message-ID: {AANLkTikoNq0MQhwanuX3FQwAJFQB7tscWZmu_MivkGTi-at-mail.gmail.com} 5, 30 -- Subject: Microtome History - was "Ernst Ruska" 5, 30 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 5, 30 -- To: microscopy-at-microscopy.com 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 37 -- From nizets2-at-yahoo.com Thu Jan 20 02:23:23 2011 22, 37 -- Received: from nm22.bullet.mail.sp2.yahoo.com (nm22.bullet.mail.sp2.yahoo.com [98.139.91.92]) 22, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0K8NNtZ004919 22, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 02:23:23 -0600 22, 37 -- Received: from [98.139.91.68] by nm22.bullet.mail.sp2.yahoo.com with NNFMP; 20 Jan 2011 08:23:22 -0000 22, 37 -- Received: from [98.139.91.46] by tm8.bullet.mail.sp2.yahoo.com with NNFMP; 20 Jan 2011 08:23:22 -0000 22, 37 -- Received: from [127.0.0.1] by omp1046.mail.sp2.yahoo.com with NNFMP; 20 Jan 2011 08:23:22 -0000 22, 37 -- X-Yahoo-Newman-Property: ymail-3 22, 37 -- X-Yahoo-Newman-Id: 470161.11129.bm-at-omp1046.mail.sp2.yahoo.com 22, 37 -- Received: (qmail 9642 invoked by uid 60001); 20 Jan 2011 08:23:22 -0000 22, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1295511802; bh=kyfQM9oYZ7mpkaZnl2ZlHNsxjOxkW2Kousf+FTUJmZc=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=TehQZal3u0PIvlqf3SJMKn9pyBjRIk9mek2tZ4PGd1riHptm11glpKrbZFqY4mpU5E+9h0WgrFVioA8qLh3WVtJheyV+mwEPjFjArJQGUTo8Mi3lKl/baFHuS/+JSj2KfZt4msqEEyihJ7YwxZ6ucLBgJzkD46Cx+NDG7Z8bdj0= 22, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 22, 37 -- s=s1024; d=yahoo.com; 22, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 22, 37 -- b=ji8Oy/V76Zql7wdUFJyHvJTfv8PTlEhC1ufjknNYF+MapzwOknKYmgG1NZOUZWv9vO0TiMQXBbELmgVNtQDO2XmUQo8lHgbHfC3TtW8b9Hi3osTvy0she2K5eU+xgtqF4ESzh3JCHGV8LjsMIa+Hzo3eoTwCEd3448Ohxc1ylUk=; 22, 37 -- Message-ID: {29284.71187.qm-at-web110809.mail.gq1.yahoo.com} 22, 37 -- X-YMail-OSG: Hx4RKFEVM1k3oNyJdDdQbLzkQb33PemNNrMffbkDFZJr66h 22, 37 -- oNFUn.ipNwhvHnEDcyJc6n_BLxGkeFKgZL8GK44XECr7lwThBIIy6y1KrTFN 22, 37 -- v0k_8dE2uECuSfjCkhzla5uvJOpM.MYnGBo73NFYZdIqANt4u7lt9HwdmzU9 22, 37 -- BvptCgpQq2.lh4kzaf.XZxNFeV2HXBq4EvcAX1dywxWolvjTHIkJzRAuAQ1Y 22, 37 -- sNuzMNsHJ_vG9ZrxDcMiqSpUuxrvFb7G4JEgvpfK3ig8pcNkRkwiQciGwhy7 22, 37 -- pGz7VSxBkHs2OHzM7NhMYm1CH9DiOdctG656Yvxs4k8dzGj2MzUNjrso5xF4 22, 37 -- iP5RKddzspm1bulAxbYJHV1M_y10uLp.dvtIkZBdOiajWOFOdtnqi3cueurj 22, 37 -- d7o.2MpgneKs0w_o- 22, 37 -- Received: from [80.122.101.100] by web110809.mail.gq1.yahoo.com via HTTP; Thu, 20 Jan 2011 00:23:21 PST 22, 37 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.107.285259 22, 37 -- References: {201101200551.p0K5ptl3024149-at-ns.microscopy.com} 22, 37 -- Date: Thu, 20 Jan 2011 00:23:21 -0800 (PST) 22, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 22, 37 -- Subject: Re: [Microscopy] Microtome History - was "Ernst Ruska" 22, 37 -- To: joelsheffield-at-gmail.com 22, 37 -- Cc: microscopy-at-microscopy.com 22, 37 -- In-Reply-To: {201101200551.p0K5ptl3024149-at-ns.microscopy.com} 22, 37 -- MIME-Version: 1.0 22, 37 -- Content-Type: text/plain; charset=iso-8859-1 22, 37 -- Content-Transfer-Encoding: 8bit 22, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0K8NNtZ004919 ==============================End of - Headers==============================
Von: Muß Wolfgang Gesendet: Donnerstag, 20. Jänner 2011 11:46 An: 'joelsheffield-at-gmail.com' Cc: 'microscopy-at-microscopy.com' Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
Joel wrote: } Now, the urban legend. I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections. How many strokes, in which direction, which side of the blade, etc. During the discussion, a bottle of Coke was knocked off the table and broke into many pieces. The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone? {
Joel
Good morning, Dear Joel, dear all
I can't tell or verify the above mentioned "Coke-shard-story" but - indeed - I can add information as to the "knocked off table"-part....
I had always heared (personal informations from elder REICHERT-freaks and service people, from lectures/lecturers in the 70ies and 80ies as well as personally from H. Sitte himself) that the group around H. SITTE at REICHERT (now LEICA) in the late 1950ies/60ies was/were doing such experiments with {glass plates} .... which were thrown down to the floor... and one of them (it was Sitte himself, as he told me) picked up the shards evaluating the edges of "the most promising one" for sectioning purposes (who of that group had the "invention" to use glass as a} crude { knife (element) I unfortunately don't know.
By the way, digging a little bit on this in Google, I found one hint on a document in a German data bank at ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.
{Ein einfaches Ultramikrotom mit thermischem Vorschub} veröffentlicht ("A simple ultramicrotome with thermal advance" published) in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
Note: {Naturwissenschaften} is the former title of Biomedical and Life Sciences (SPRINGER) Another source: http://www.freepatentsonline.com/3828641.html: United States Patent US3828641:
==} Apparatus for adjusting the elevation of a specimen in microtomes, particularly ultramicrotomes Inventor: Hellmuth Sitte, HOMBURG (not Humburg [as written in the patent document!]/Saar Germany) Assignee: C.Reichert Optische Werke AG, Vienna, Austria) Filed: Nov. 10,1972
Also see http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false (From the book Michael J. Dykstra, Laura E. Reuss - 2003 (see page "History: in the text displayed the Reichert OMU-1 (before 1964, perhaps see document above in {Naturwissenschaften} 1955) from Sitte described as having thermal advance, Sitte was never happy with, since 1964: OMU-2 with thermal advance....
This just for your pleasure.... (and I sure that searching for Sitte Hellmuth or Sitte and microtome will find some results more on that interesting and exciting "history" of ultramicrotomy advances.....
Best wishes and regards, have a good and successful rest of the week and a beautiful weekend,
Wolfgang MUSS (Ph.D.) EM-Lab Gen.Hosp. SALK-LKH SALZBURG AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } Gesendet: Donnerstag, 20. Jänner 2011 06:51 } An: Muß Wolfgang } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } It is certainly clear that there are many interesting stories --some } urban legends, and some insights into the minds of those that founded } the discipline of thin section electron microscopy. } During the mid '70's, I taught an EM course at Rutgers, Camden. } In the basement, along with an RCA EMU-2 (!) was a thermal advance } microtome that I was told was developed by Sjostrand. } This thing consisted of a disk-like plate, about 4-5 inches in } diameter, with an eccentrically mounted arm that stuck out of the } plate. The arm had a chuck for a tissue sample, and a heating coil. } The plate itself was mounted in a frame and lubricated with oil --the } plate acted like a large oil bearing. The plate was linked to a motor } on the wall by a long V belt (to minimize vibration), and the entire } plate rotated, bringing the block past the knife on each rotation. The } sections that resulted tended to be arc shaped. We got it working, but } it was a terrifying thing to see. I wonder if anyone in this group } remembers this type of machine -- assuming that my description is } comprehensible. } } The other microtome that I used, and really admire, was the Huxley } microtome, originally sold by Cambridge, and ultimately marketed by } LKB. Although it used a mechanical advance, all of the movements of } the cutting arm were made by bending sheets of spring steel, so that } there were no surfaces that moved against each other. Moreover, the } cutting stroke was controlled by an oil-filled damper, to minimize } chatter. -- a remarkable and stable machine. } } Now, the urban legend. } I had heard that a group (unnamed) was having a vigorous discussion } about the best way to strop a steel knife so as to get the best edge } for cutting thin sections. How many strokes, in which direction, which } side of the blade, etc. During the discussion, a bottle of Coke was } knocked off the table and broke into many pieces. } The story goes that one, or another, of these people took up one of the } shards, and suggested that it would make a good knife edge. } Verification anyone? } } Joel } } -- } Joel B. Sheffield, Ph.D. } Biology Department, Temple University } 1900 North 12th Street } Philadelphia, PA 19122 } jbs-at-temple.edu } (215) 204 8839, fax (215) 204 0486 } http://astro.temple.edu/~jbs } } ==============================Original } Headers============================== } 5, 30 -- From joelsheffield-at-gmail.com Wed Jan 19 23:47:26 2011 } 5, 30 -- Received: from mail-ww0-f49.google.com (mail-ww0- } f49.google.com [74.125.82.49]) } 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p0K5lQgB018304 } 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 23:47:26 } -0600 } 5, 30 -- Received: by wwb17 with SMTP id 17so256663wwb.18 } 5, 30 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 } 21:47:25 -0800 (PST) } 5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 30 -- d=gmail.com; s=gamma; } 5, 30 -- h=domainkey-signature:mime-version:date:message- } id:subject:from:to } 5, 30 -- :content-type; } 5, 30 -- bh=kPUiuV1UxIO0H1pRVf1DSBLH7XQoy5aO2bN7+G+szxM=; } 5, 30 -- } b=UXJCg0QqUwPbZUaTOkLx3i+Tq22ZLycH3163aMjr/YaOTUlNXA+V3Uah88ZvAbPim3 } 5, 30 -- } F7lWxUBBph/7Gqa/wiZDTEikXy4U+eCZ5a2FDRoLqH2T23rZ0bmrhTcekQ2FpBKKwEtC } 5, 30 -- cMlEC0ztRmc2ZtJPpd93Zra4IQSLR9zEbBxYE= } 5, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 5, 30 -- d=gmail.com; s=gamma; } 5, 30 -- h=mime-version:date:message- } id:subject:from:to:content-type; } 5, 30 -- } b=rwKrTeNXOA45KaZiFgYiUR8hEDH/WD4BYLEe+mTGsnjAUl+zABKfV824sTmyEd7EwU } 5, 30 -- } FeXrP78Ws/O0V/Tvzil7+UlEWx3u0y+u9UG3QRXhbJtGDNRwFUpTop9td+FSdKQ8ZDmD } 5, 30 -- a/L+pGrqTp0BPxBRSJFCfG/idbLcFzFb4b6fg= } 5, 30 -- MIME-Version: 1.0 } 5, 30 -- Received: by 10.227.155.66 with SMTP id } r2mr1805845wbw.73.1295502444180; Wed, } 5, 30 -- 19 Jan 2011 21:47:24 -0800 (PST) } 5, 30 -- Received: by 10.227.143.213 with HTTP; Wed, 19 Jan 2011 } 21:47:24 -0800 (PST) } 5, 30 -- Date: Thu, 20 Jan 2011 00:47:24 -0500 } 5, 30 -- Message-ID: } {AANLkTikoNq0MQhwanuX3FQwAJFQB7tscWZmu_MivkGTi-at-mail.gmail.com} } 5, 30 -- Subject: Microtome History - was "Ernst Ruska" } 5, 30 -- From: Joel Sheffield {joelsheffield-at-gmail.com} } 5, 30 -- To: microscopy-at-microscopy.com } 5, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - } Headers==============================
==============================Original Headers============================== 26, 35 -- From W.Muss-at-salk.at Thu Jan 20 04:47:01 2011 26, 35 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 26, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KAl0Sn024668 26, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 04:47:00 -0600 26, 35 -- Received: from localhost (localhost [127.0.0.1]) 26, 35 -- by hermes.salk.at (Postfix) with ESMTP id 7BC3AC386B 26, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:46:59 +0100 (CET) 26, 35 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 26, 35 -- Received: from hermes.salk.at ([127.0.0.1]) 26, 35 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 26, 35 -- with ESMTP id u1-3Xessr7tn for {microscopy-at-microscopy.com} ; 26, 35 -- Thu, 20 Jan 2011 11:46:59 +0100 (CET) 26, 35 -- Received: from n1rz122.lksdom21.lks.local (n1rz122.lksdom21.lks.local [192.168.101.122]) 26, 35 -- by hermes.salk.at (Postfix) with ESMTP id 0E565C3844 26, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:46:59 +0100 (CET) 26, 35 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 26, 35 -- Thu, 20 Jan 2011 11:46:58 +0100 26, 35 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 35 -- Content-class: urn:content-classes:message 26, 35 -- MIME-Version: 1.0 26, 35 -- Content-Type: text/plain; 26, 35 -- charset="iso-8859-1" 26, 35 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" 26, 35 -- Date: Thu, 20 Jan 2011 11:46:58 +0100 26, 35 -- Message-ID: {06B4ED29F824524E98E8AA5BB640706293966C-at-N1RZ116.lksdom21.lks.local} 26, 35 -- X-MS-Has-Attach: 26, 35 -- X-MS-TNEF-Correlator: 26, 35 -- Thread-Topic: [Microscopy] Re: Microtome History - was "Ernst Ruska" 26, 35 -- Thread-Index: Acu4Zgs06778XDVBTg2CGrAzFhN94wAH0u6QAAJ7SGA= 26, 35 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 26, 35 -- To: {microscopy-at-microscopy.com} 26, 35 -- X-OriginalArrivalTime: 20 Jan 2011 10:46:58.0664 (UTC) FILETIME=[5CC80E80:01CBB88F] 26, 35 -- X-Scanned-By: SALK-Content-Filter 26, 35 -- Content-Transfer-Encoding: 8bit 26, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KAl0Sn024668 ==============================End of - Headers==============================
Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter.
You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe.
Does anyone know if this means the sample can be any size one likes?
Dave
-----Original Message----- X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] Sent: 19 January 2011 22:53 To: David Patton
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin Foley
Organization: UAB
Title-Subject: [Filtered] Environmental SEM for Biological Work
Message: Is there heavy use of ESEM for biological SEM? What is commonly done on biological ESEM samples? We're looking at purchasing an environmental SEM and the sales rep. tells us we will need a Peltier stage to cool the sample to look at wet samples. Unfortunately, the maximum sample size for the Peltier stage is 3 mm across. This seems tiny to me for an SEM. Are there many biological ESEM applications that don't require the sample to be wet?
Thanks,
Robin Foley (who spends most of her SEM time looking at metals, ceramics and polymers!)
I was taught decades ago that early ultrathin sections were made in the following manner:
A sheet of plate glass was dropped on the floor. The shard with the best edge was selected and attached to the blade of a small window fan. The resin-embedded specimen was attached to the tip of a soldering iron. once everything was clamped and aligned, the fan was turned on, the soldering iron was plugged in, and the section were collected in a large pan of water held beneath the fan. (As the soldering iron heated, the thermal expansion advanced the specimen into the glass blade.) Sections of optimal thickness were selected from those floating in the pan on the basis of the color refracted.
I love the story. Does anyone know whether this even approaches a true story?
Thanks,
Don
P.S. Anyone still alive who has first-hand knowledge of this story would be pretty old by now, but I thought that I would give this a shot.
--
Donald L. Lovett e-mail: lovett-at-tcnj.edu Professor and Chairperson phone: 609-771-2876 Department of Biology fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
==============================Original Headers============================== 10, 28 -- From lovett-at-tcnj.edu Thu Jan 20 08:19:04 2011 10, 28 -- Received: from mailgate-too.TCNJ.EDU (mailgate-too.TCNJ.EDU [159.91.13.67]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEJ4dB030331 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:19:04 -0600 10, 28 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209]) 10, 28 -- by mailgate-too.TCNJ.EDU (Postfix) with ESMTP id 58885FF57E 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:13:11 -0500 (EST) 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id CAF513373AB 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU 10, 28 -- Received: from zcs.tcnj.edu ([127.0.0.1]) 10, 28 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id fQjpUdFveSTN for {Microscopy-at-microscopy.com} ; 10, 28 -- Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Received: from [159.91.213.72] (unknown [159.91.213.72]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id AF779337393 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Message-ID: {4D384456.6040204-at-tcnj.edu} 10, 28 -- Date: Thu, 20 Jan 2011 09:19:02 -0500 10, 28 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu} 10, 28 -- Organization: Department of Biology, The College of New Jersey 10, 28 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 28 -- MIME-Version: 1.0 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Subject: Early microtomy--urban legend? 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Anthony, If you don't have a cold stage on the FESEM, I would suggest finding some faithful old tungsten SEM that can stand the abuse of wax evaporating under the beam. That will also go a long way towards staying in the good graces of whoever is in charge of the FESEM.
If you have a cold stage, then others on the list can probably give you some help on that score.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message-----
X-from: anthonyribaudo3-at-gmail.com [mailto:anthonyribaudo3-at-gmail.com] Sent: Wednesday, January 19, 2011 3:36 PM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both anthonyribaudo3-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: anthonyribaudo3-at-gmail.com Name: Anthony Ribaudo
Organization: TRI Princeton
Title-Subject: [Filtered] SEM imaging of wax
Message: Can anyone suggest guidelines for imaging a paraffin wax coating on a metal or polymeric substrate via FESEM. The plan is to detect the wax layer that is thought to be several hundred nanometers thick? Are there any waxes that would be more stable to image under the electron beam than paraffin wax?
I have heard all the microtome and glass knives stories also but have no evidence to their veracity. But I once was talking to Sanford Palay - an early pioneer in EM - and he told me how they used a rubber hammer to tap the outside of the TEM column to try and nudge the aperture into place. I can't imagine how tedious that must have been. Of course, the reward the pioneers all got from primitive microtomy and TEMs was that they got to discover amazing new things of a bigger magnitude than most of the incremental advances we all get to contribute to these days. But I think I would rather have done science then rather than now when high cost forces specialization and a greater proportion of time is devoted to finding funding rather than organelles. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu] Sent: Thursday, January 20, 2011 8:20 AM To: Phillips, Thomas E.
Fellow microscopists:
I was taught decades ago that early ultrathin sections were made in the following manner:
A sheet of plate glass was dropped on the floor. The shard with the best edge was selected and attached to the blade of a small window fan. The resin-embedded specimen was attached to the tip of a soldering iron. once everything was clamped and aligned, the fan was turned on, the soldering iron was plugged in, and the section were collected in a large pan of water held beneath the fan. (As the soldering iron heated, the thermal expansion advanced the specimen into the glass blade.) Sections of optimal thickness were selected from those floating in the pan on the basis of the color refracted.
I love the story. Does anyone know whether this even approaches a true story?
Thanks,
Don
P.S. Anyone still alive who has first-hand knowledge of this story would be pretty old by now, but I thought that I would give this a shot.
--
Donald L. Lovett e-mail: lovett-at-tcnj.edu Professor and Chairperson phone: 609-771-2876 Department of Biology fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
==============================Original Headers============================== 10, 28 -- From lovett-at-tcnj.edu Thu Jan 20 08:19:04 2011 10, 28 -- Received: from mailgate-too.TCNJ.EDU (mailgate-too.TCNJ.EDU [159.91.13.67]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEJ4dB030331 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:19:04 -0600 10, 28 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209]) 10, 28 -- by mailgate-too.TCNJ.EDU (Postfix) with ESMTP id 58885FF57E 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:13:11 -0500 (EST) 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id CAF513373AB 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU 10, 28 -- Received: from zcs.tcnj.edu ([127.0.0.1]) 10, 28 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id fQjpUdFveSTN for {Microscopy-at-microscopy.com} ; 10, 28 -- Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Received: from [159.91.213.72] (unknown [159.91.213.72]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id AF779337393 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Message-ID: {4D384456.6040204-at-tcnj.edu} 10, 28 -- Date: Thu, 20 Jan 2011 09:19:02 -0500 10, 28 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu} 10, 28 -- Organization: Department of Biology, The College of New Jersey 10, 28 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 28 -- MIME-Version: 1.0 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Subject: Early microtomy--urban legend? 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 32 -- From PhillipsT-at-missouri.edu Thu Jan 20 08:54:00 2011 19, 32 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KErxrG030207 19, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:54:00 -0600 19, 32 -- X-IronPort-Anti-Spam-Filtered: true 19, 32 -- X-IronPort-Anti-Spam-Result: AvsEAN7aN03RauUo/2dsb2JhbACkUXO1cYhEAoMQgj4EhG+BN4Qsg3Y 19, 32 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.229.40]) 19, 32 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 19, 32 -- Thu, 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 19, 32 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, 20 Jan 2011 19, 32 -- 08:53:58 -0600 19, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 19, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 32 -- Date: Thu, 20 Jan 2011 08:53:54 -0600 19, 32 -- Subject: RE: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Topic: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Index: Acu4rTCfGg2oH1rLRqikoaKr1g1gsgAA+QUQ 19, 32 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF1A-at-UM-EMAIL05.um.umsystem.edu} 19, 32 -- References: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- In-Reply-To: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- Accept-Language: en-US 19, 32 -- Content-Language: en-US 19, 32 -- X-MS-Has-Attach: 19, 32 -- X-MS-TNEF-Correlator: 19, 32 -- acceptlanguage: en-US 19, 32 -- Content-Type: text/plain; charset="us-ascii" 19, 32 -- MIME-Version: 1.0 19, 32 -- X-OriginalArrivalTime: 20 Jan 2011 14:53:59.0017 (UTC) FILETIME=[DE667590:01CBB8B1] 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KErxrG030207 ==============================End of - Headers==============================
The University of Florida, College of Medicine, Department of Biochemistry and Molecular Biology seeks an enthusiastic Ph.D. level structural biologist, with experience in the area of cryo-electron microscopy and/or X-ray crystallography and image reconstruction to work on the structure of ssDNA virus proteins and intact capsids. The position will require knowledge of molecular biology, biochemistry, and structural biology approaches. Training can be provided, as needed, but a basic knowledge of the technical aspects of these approaches is required. In addition, projects in the lab often require collaborative effort, thus an ability/desire to work in a group setting is preferable. Salary will be negotiable and commensurate with experience. Interested applicants should send their Curriculum Vitae plus the names of three individuals who can provide a letter of reference to Dr. Mavis Agbandje-McKenna, Department of Biochemistry and Molecular Biology, 1600 SW Archer Road, PO Box 100245, Gainesville, FL 32610-0245 or email to mckenna-at-ufl.edu . The University of Florida is an Equal Opportunity Employee.
Thanks,
Byung-Ho Kang, Ph.D. Assistant Professor, Microbiology and Cell Science Director, Electron Microscopy and Bioimaging Lab, Interdisciplinary Center for Biotechnology Research University of Florida Gainesville, FL 32611 Tel: 352-846-0952 Fax: 352-392-5922
==============================Original Headers============================== 6, 26 -- From bkang-at-ufl.edu Thu Jan 20 08:54:46 2011 6, 26 -- Received: from smtp.ufl.edu (smtp03.osg.ufl.edu [128.227.74.70]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEsj1f031129 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:54:45 -0600 6, 26 -- Received: from [10.249.16.125] ([10.249.16.125]) 6, 26 -- (authenticated bits=0) 6, 26 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id p0KEshlY017413 6, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:54:44 -0500 6, 26 -- From: Byung-Ho Kang {bkang-at-ufl.edu} 6, 26 -- Content-Type: text/plain; charset=us-ascii 6, 26 -- Subject: Postdoctoral Research Associate Position available at the University of Florida 6, 26 -- Date: Thu, 20 Jan 2011 09:54:43 -0500 6, 26 -- Message-Id: {AF086E19-9824-4374-ACB7-4992258BA3D1-at-ufl.edu} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 26 -- X-Mailer: Apple Mail (2.1082) 6, 26 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 6, 26 -- definitions=2011-01-20_04:2011-01-20,2011-01-20,1970-01-01 signatures=0 6, 26 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=1 6, 26 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 6, 26 -- engine=6.0.2-1012030000 definitions=main-1101200053 6, 26 -- X-Spam-Level: * 6, 26 -- X-UFL-Spam-Level: * 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KEsj1f031129 ==============================End of - Headers==============================
Just a question disguised in an answer: wouldn't it be the right application to make a replica?
Stephane
----- Original Message ---- X-from: "anthonyribaudo3-at-gmail.com" {anthonyribaudo3-at-gmail.com} To: nizets2-at-yahoo.com Sent: Wed, January 19, 2011 9:39:49 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both anthonyribaudo3-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: anthonyribaudo3-at-gmail.com Name: Anthony Ribaudo
Organization: TRI Princeton
Title-Subject: [Filtered] SEM imaging of wax
Message: Can anyone suggest guidelines for imaging a paraffin wax coating on a metal or polymeric substrate via FESEM. The plan is to detect the wax layer that is thought to be several hundred nanometers thick? Are there any waxes that would be more stable to image under the electron beam than paraffin wax?
Forgot how much fun these discussions of the "old days" can be. At Washington University there was an old Seimens microscope. At the time I was training as a tech, and using the microscope was not an option. However, the man who trained me, Charles Kuhn, told me that one of the microscopes he had used, and I believe he was referring to the old Seimens at WU, had to be aligned using a rubber mallet. Never did it personally, but was regaled with stories by one who did.
paul -- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 3, 21 -- From paul_hazelton-at-umanitoba.ca Thu Jan 20 09:36:45 2011 3, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFaitU013986 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:36:44 -0600 3, 21 -- Received: from [140.193.25.116] (basic116.medmb.umanitoba.ca [140.193.25.116]) 3, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) 3, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0KFahNr022457 3, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); 3, 21 -- Thu, 20 Jan 2011 09:36:43 -0600 (CST) 3, 21 -- Message-ID: {4D38568A.2050009-at-umanitoba.ca} 3, 21 -- Date: Thu, 20 Jan 2011 09:36:42 -0600 3, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 3, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 3, 21 -- MIME-Version: 1.0 3, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 21 -- Subject: Re: [Microscopy] RE: Early microtomy--urban legend? 3, 21 -- References: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- In-Reply-To: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
I know at least 2 old microscopists who made glass knifes by breaking window glass - one of them here at CMU. He went to construction sites to get the broken windows.
I'm running down the reference and (I hope) an image, but one of the early tries at ultramicrotomy was sticking razor blades on a centrifuge rotor, then mount the sections on the tub, close the lid and turn on the centrifuge. The sections where then picked up from inside the tub, having been flung willy-nilly around the inside. (The image is used in the microtomy lecture to convince the students thin sectioning could be a lot worse than they think it is.)
Phil
} Von: Muþ Wolfgang } Gesendet: Donnerstag, 20. J”nner 2011 11:46 } An: 'joelsheffield-at-gmail.com' } Cc: 'microscopy-at-microscopy.com' } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } Joel wrote: } } Now, the urban legend.Ý } I had heard that a group (unnamed) was having a } vigorous discussion about the best way to strop } a steel knife so as to get the best edge for } cutting thin sections.Ý How many strokes, in } which direction, which side of the blade, etc.Ý } During the discussion, a bottle of Coke was } knocked off the table and broke into many } pieces.Ý } The story goes that one, or another, of these } people took up one of the shards, and suggested } that it would make a good knife edge. } Verification anyone? { } } Joel } } } Good morning, } Dear Joel, dear all } } I can't tell or verify the above mentioned } "Coke-shard-story" but - indeed - I can add } information as to the "knocked off } table"-part.... } } I had always heared (personal informations from } elder REICHERT-freaks and service people, from } lectures/lecturers in the 70ies and 80ies as } well as personally from H. Sitte himself) that } the group around H. SITTE at REICHERT (now } LEICA) in the late 1950ies/60ies was/were doing } such experiments with {glass plates} .... which } were thrown down to the floor... and one of them } (it was Sitte himself, as he told me) picked up } the shards evaluating the edges of "the most } promising one" for sectioning purposes (who of } that group had the "invention" to use glass as } a} crude { knife (element) I unfortunately don't } know. } } By the way, digging a little bit on this in } Google, I found one hint on a document in a } German data bank at } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver–ffentlicht } ("A simple ultramicrotome with thermal advance" published) } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } Note:Ý {Naturwissenschaften} is the former title } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } Another source:Ý http://www.freepatentsonline.com/3828641.html: } United States Patent US3828641: } } ==} Apparatus for adjusting the elevation of a } specimen in microtomes, particularly } ultramicrotomes } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } [as written in the patent document!]/Saar } Germany) } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } Filed:Ý Nov. 10,1972 } } Also see } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } (From the book Michael J. Dykstra, Laura E. } Reuss - 2003Ý (see page "History: in the text } displayed } the Reichert OMU-1 (before 1964, perhaps see } document above in {Naturwissenschaften} 1955) } from Sitte described as having thermal advance, } Sitte was never happy with, since 1964: OMU-2 } with thermal advance.... } } This just for your pleasure.... } (and I sure that searching for Sitte Hellmuth or } Sitte and microtome will find some results more } on that interesting and exciting "history" of } ultramicrotomy advances..... } } Best wishes and regards, } have a good and successful rest of the week and a beautiful weekend, } } Wolfgang MUSS (Ph.D.) } EM-Lab Gen.Hosp. SALK-LKH } SALZBURG } AUSTRIA } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } Gesendet: Donnerstag, 20. J”nner 2011 06:51 } } An: Muþ Wolfgang } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } ----------------------------------------------------------------------- } } ----- } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } America } } ToÝ Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } ----- } } } } It is certainly clear that there are many interesting stories --some } } urban legends, and some insights into the minds of those that founded } } the discipline of thin section electron microscopy. } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } microtome that I was told was developed by Sjostrand. } } This thing consisted of a disk-like plate, about 4-5 inches in } } diameter, with an eccentrically mounted arm that stuck out of the } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } The plate itself was mounted in a frame and lubricated with oil --the } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } on the wall by a long V belt (to minimize vibration), and the entire } } plate rotated, bringing the block past the knife on each rotation.Ý The } } sections that resulted tended to be arc shaped.Ý We got it working, but } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } remembers this type of machine -- assuming that my description is } } comprehensible. } } } } The other microtome that I used, and really admire, was the Huxley } } microtome, originally sold by Cambridge, and ultimately marketed by } } LKB.Ý Although it used a mechanical advance, all of the movements of } } the cutting arm were made by bending sheets of spring steel, so that } } there were no surfaces that moved against each other.Ý Moreover, the } } cutting stroke was controlled by an oil-filled damper, to minimize } } chatter. -- a remarkable and stable machine. } } } } Now, the urban legend. } } I had heard that a group (unnamed) was having a vigorous discussion } } about the best way to strop a steel knife so as to get the best edge } } for cutting thin sections.Ý How many strokes, in which direction, which } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } knocked off the table and broke into many pieces. } } The story goes that one, or another, of these people took up one of the } } shards, and suggested that it would make a good knife edge. } } Verification anyone? } } } } Joel } } } } -- } } Joel B. Sheffield, Ph.D. } } Biology Department, Temple University } } 1900 North 12th Street } } Philadelphia, PA 19122 } } jbs-at-temple.edu } } (215) 204 8839, fax (215) 204 0486 } } http://astro.temple.edu/~jbs
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 6, 27 -- Mime-Version: 1.0 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 6, 27 -- To: Microscopy-at-microscopy.com 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 ==============================End of - Headers==============================
I just remembered seeing a print ad from some science journal published the 40's or 50's (before my time!) for an early ultramicrotome. It was a high speed motor spinning some type of blade. The concept was that the block was advanced into this buzzsaw and you were supposed to catch the sections flying off. At the time I think the view was that ultrathins could only be cut at high speed. The real kicker was the ad mentioned the motor was also suitable for use in centrifuges. Crazy. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, January 20, 2011 9:54 AM To: Phillips, Thomas E.
I know at least 2 old microscopists who made glass knifes by breaking window glass - one of them here at CMU. He went to construction sites to get the broken windows.
I'm running down the reference and (I hope) an image, but one of the early tries at ultramicrotomy was sticking razor blades on a centrifuge rotor, then mount the sections on the tub, close the lid and turn on the centrifuge. The sections where then picked up from inside the tub, having been flung willy-nilly around the inside. (The image is used in the microtomy lecture to convince the students thin sectioning could be a lot worse than they think it is.)
Phil
} Von: Muþ Wolfgang } Gesendet: Donnerstag, 20. J"nner 2011 11:46 } An: 'joelsheffield-at-gmail.com' } Cc: 'microscopy-at-microscopy.com' } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } Joel wrote: } } Now, the urban legend.Ý } I had heard that a group (unnamed) was having a } vigorous discussion about the best way to strop } a steel knife so as to get the best edge for } cutting thin sections.Ý How many strokes, in } which direction, which side of the blade, etc.Ý } During the discussion, a bottle of Coke was } knocked off the table and broke into many } pieces.Ý } The story goes that one, or another, of these } people took up one of the shards, and suggested } that it would make a good knife edge. } Verification anyone? { } } Joel } } } Good morning, } Dear Joel, dear all } } I can't tell or verify the above mentioned } "Coke-shard-story" but - indeed - I can add } information as to the "knocked off } table"-part.... } } I had always heared (personal informations from } elder REICHERT-freaks and service people, from } lectures/lecturers in the 70ies and 80ies as } well as personally from H. Sitte himself) that } the group around H. SITTE at REICHERT (now } LEICA) in the late 1950ies/60ies was/were doing } such experiments with {glass plates} .... which } were thrown down to the floor... and one of them } (it was Sitte himself, as he told me) picked up } the shards evaluating the edges of "the most } promising one" for sectioning purposes (who of } that group had the "invention" to use glass as } a} crude { knife (element) I unfortunately don't } know. } } By the way, digging a little bit on this in } Google, I found one hint on a document in a } German data bank at } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht } ("A simple ultramicrotome with thermal advance" published) } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } Note:Ý {Naturwissenschaften} is the former title } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } Another source:Ý http://www.freepatentsonline.com/3828641.html: } United States Patent US3828641: } } ==} Apparatus for adjusting the elevation of a } specimen in microtomes, particularly } ultramicrotomes } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } [as written in the patent document!]/Saar } Germany) } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } Filed:Ý Nov. 10,1972 } } Also see } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } (From the book Michael J. Dykstra, Laura E. } Reuss - 2003Ý (see page "History: in the text } displayed } the Reichert OMU-1 (before 1964, perhaps see } document above in {Naturwissenschaften} 1955) } from Sitte described as having thermal advance, } Sitte was never happy with, since 1964: OMU-2 } with thermal advance.... } } This just for your pleasure.... } (and I sure that searching for Sitte Hellmuth or } Sitte and microtome will find some results more } on that interesting and exciting "history" of } ultramicrotomy advances..... } } Best wishes and regards, } have a good and successful rest of the week and a beautiful weekend, } } Wolfgang MUSS (Ph.D.) } EM-Lab Gen.Hosp. SALK-LKH } SALZBURG } AUSTRIA } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } Gesendet: Donnerstag, 20. J"nner 2011 06:51 } } An: Muþ Wolfgang } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } ----------------------------------------------------------------------- } } ----- } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } America } } ToÝ Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } ----- } } } } It is certainly clear that there are many interesting stories --some } } urban legends, and some insights into the minds of those that founded } } the discipline of thin section electron microscopy. } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } microtome that I was told was developed by Sjostrand. } } This thing consisted of a disk-like plate, about 4-5 inches in } } diameter, with an eccentrically mounted arm that stuck out of the } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } The plate itself was mounted in a frame and lubricated with oil --the } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } on the wall by a long V belt (to minimize vibration), and the entire } } plate rotated, bringing the block past the knife on each rotation.Ý The } } sections that resulted tended to be arc shaped.Ý We got it working, but } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } remembers this type of machine -- assuming that my description is } } comprehensible. } } } } The other microtome that I used, and really admire, was the Huxley } } microtome, originally sold by Cambridge, and ultimately marketed by } } LKB.Ý Although it used a mechanical advance, all of the movements of } } the cutting arm were made by bending sheets of spring steel, so that } } there were no surfaces that moved against each other.Ý Moreover, the } } cutting stroke was controlled by an oil-filled damper, to minimize } } chatter. -- a remarkable and stable machine. } } } } Now, the urban legend. } } I had heard that a group (unnamed) was having a vigorous discussion } } about the best way to strop a steel knife so as to get the best edge } } for cutting thin sections.Ý How many strokes, in which direction, which } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } knocked off the table and broke into many pieces. } } The story goes that one, or another, of these people took up one of the } } shards, and suggested that it would make a good knife edge. } } Verification anyone? } } } } Joel } } } } -- } } Joel B. Sheffield, Ph.D. } } Biology Department, Temple University } } 1900 North 12th Street } } Philadelphia, PA 19122 } } jbs-at-temple.edu } } (215) 204 8839, fax (215) 204 0486 } } http://astro.temple.edu/~jbs
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 6, 27 -- Mime-Version: 1.0 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 6, 27 -- To: Microscopy-at-microscopy.com 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 35 -- From PhillipsT-at-missouri.edu Thu Jan 20 10:20:18 2011 16, 35 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KGKG9E014299 16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:20:16 -0600 16, 35 -- X-IronPort-Anti-Spam-Filtered: true 16, 35 -- X-IronPort-Anti-Spam-Result: AvsEAH7vN03RauUp/2dsb2JhbACkUXO2AYheAoJuIoI+BIRviVmCcw 16, 35 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.229.41]) 16, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 10:20:14 -0600 16, 35 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 16, 35 -- Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from UM-MSXHUB05.um.umsystem.edu (131.151.121.205) by 16, 35 -- UM-THUB01.um.umsystem.edu (209.106.230.181) with Microsoft SMTP Server (TLS) 16, 35 -- id 8.2.254.0; Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 16, 35 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Thu, 20 Jan 2011 16, 35 -- 10:19:54 -0600 16, 35 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 35 -- Date: Thu, 20 Jan 2011 10:19:53 -0600 16, 35 -- Subject: [Microscopy] Re: Microtome History 16, 35 -- Thread-Topic: [Microscopy] Re: Microtome History 16, 35 -- Thread-Index: Acu4ujnK/W+eYZMgQe2m/DmfMJG5qAAAyCyg 16, 35 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF46-at-UM-EMAIL05.um.umsystem.edu} 16, 35 -- References: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- In-Reply-To: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- Accept-Language: en-US 16, 35 -- Content-Language: en-US 16, 35 -- X-MS-Has-Attach: 16, 35 -- X-MS-TNEF-Correlator: 16, 35 -- acceptlanguage: en-US 16, 35 -- Content-Type: text/plain; charset="iso-8859-1" 16, 35 -- MIME-Version: 1.0 16, 35 -- X-OriginalArrivalTime: 20 Jan 2011 16:19:55.0726 (UTC) FILETIME=[E009CEE0:01CBB8BD] 16, 35 -- Content-Transfer-Encoding: 8bit 16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KGKG9E014299 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mmgraham06-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mmgraham06-at-gmail.com Name: Morven Graham
Organization: Yale School of Medicine
Title-Subject: [Filtered] Re:Research Assistant Position
Message: Hi Listeners
There is a current opening for a EM technician at Yale University Medical School.
I had a Topcon 002B at Intel for many years for which use of a hammer was required. The ion pump would periodically grow whiskers that resulted in markedly increased ion current. The best way to get rid of them was to bang on the pump with a hammer. I don't mean a rubber mallet either. To be fully effective, this required a big iron hammer and hearing protection, as well as thick skin to withstand the curious stares and complaints about the noise and questions about one's sanity from fellow workers.
John Mardinly
Western Digital
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: Thursday, January 20, 2011 7:44 AM To: John Mardinly
Forgot how much fun these discussions of the "old days" can be. At Washington University there was an old Seimens microscope. At the time I was training as a tech, and using the microscope was not an option. However, the man who trained me, Charles Kuhn, told me that one of the microscopes he had used, and I believe he was referring to the old Seimens at WU, had to be aligned using a rubber mallet. Never did it personally, but was regaled with stories by one who did.
paul -- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 3, 21 -- From paul_hazelton-at-umanitoba.ca Thu Jan 20 09:36:45 2011 3, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFaitU013986 3, 21 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:36:44 -0600 3, 21 -- Received: from [140.193.25.116] (basic116.medmb.umanitoba.ca [140.193.25.116]) 3, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) 3, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p0KFahNr022457 3, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); 3, 21 -- Thu, 20 Jan 2011 09:36:43 -0600 (CST) 3, 21 -- Message-ID: {4D38568A.2050009-at-umanitoba.ca} 3, 21 -- Date: Thu, 20 Jan 2011 09:36:42 -0600 3, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 3, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 3, 21 -- MIME-Version: 1.0 3, 21 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 21 -- Subject: Re: [Microscopy] RE: Early microtomy--urban legend? 3, 21 -- References: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- In-Reply-To: {201101201456.p0KEu0Bl002900-at-ns.microscopy.com} 3, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 21 -- Content-Transfer-Encoding: 7bit 3, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
==============================Original Headers============================== 12, 30 -- From prvs=994c3f914=John.Mardinly-at-wdc.com Thu Jan 20 11:10:24 2011 12, 30 -- Received: from wdscspam2.wdc.com (wdscspam2.wdc.com [129.253.55.43]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHAMhs015780 12, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:10:23 -0600 12, 30 -- X-IronPort-AV: E=Sophos;i="4.60,352,1291622400"; 12, 30 -- d="scan'208";a="172510791" 12, 30 -- Received: from unknown (HELO wdscexfe02.sc.wdc.com) ([172.25.19.2]) 12, 30 -- by wdscspam2.wdc.com with ESMTP; 20 Jan 2011 09:10:15 -0800 12, 30 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe02.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.4675); 12, 30 -- Thu, 20 Jan 2011 09:10:19 -0800 12, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 30 -- Content-class: urn:content-classes:message 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; 12, 30 -- charset="us-ascii" 12, 30 -- Subject: Hammer Required 12, 30 -- Date: Thu, 20 Jan 2011 09:10:19 -0800 12, 30 -- Message-ID: {34061960C62E274C8AF1DCAA65655558097AA6D7-at-wdksjexbe01.msj.wdc.com} 12, 30 -- In-Reply-To: {201101201544.p0KFiE15027036-at-ns.microscopy.com} 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- Thread-Topic: Hammer Required 12, 30 -- Thread-Index: Acu4uOR02gkLoEhtTJeOGW8TB0f+tgAC1YWg 12, 30 -- References: {201101201544.p0KFiE15027036-at-ns.microscopy.com} 12, 30 -- From: "John Mardinly" {John.Mardinly-at-wdc.com} 12, 30 -- To: {paul_hazelton-at-umanitoba.ca} 12, 30 -- Cc: {Microscopy-at-microscopy.com} 12, 30 -- X-OriginalArrivalTime: 20 Jan 2011 17:10:19.0902 (UTC) FILETIME=[EA9671E0:01CBB8C4] 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHAMhs015780 ==============================End of - Headers==============================
Indeed. We are in a building that was often broken into. Years ago, I made it my business to collect the old door panes --they were a tinted glass, about 1/4" thick, and had just the right temper to make excellent knives.
Joel
On Thu, Jan 20, 2011 at 10:59 AM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I know at least 2 old microscopists who made } glass knifes by breaking window glass - one of } them here at CMU. He went to construction sites } to get the broken windows. } } I'm running down the reference and (I hope) an } image, but one of the early tries at } ultramicrotomy was sticking razor blades on a } centrifuge rotor, then mount the sections on the } tub, close the lid and turn on the centrifuge. } The sections where then picked up from inside the } tub, having been flung willy-nilly around the } inside. } (The image is used in the microtomy lecture to } convince the students thin sectioning could be a } lot worse than they think it is.) } } Phil } } } Von: Muþ Wolfgang } } Gesendet: Donnerstag, 20. J”nner 2011 11:46 } } An: 'joelsheffield-at-gmail.com' } } Cc: 'microscopy-at-microscopy.com' } } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } Joel wrote: } } } Now, the urban legend.Ý } } I had heard that a group (unnamed) was having a } } vigorous discussion about the best way to strop } } a steel knife so as to get the best edge for } } cutting thin sections.Ý How many strokes, in } } which direction, which side of the blade, etc.Ý } } During the discussion, a bottle of Coke was } } knocked off the table and broke into many } } pieces.Ý } } The story goes that one, or another, of these } } people took up one of the shards, and suggested } } that it would make a good knife edge. } } Verification anyone? { } } } } Joel } } } } } } Good morning, } } Dear Joel, dear all } } } } I can't tell or verify the above mentioned } } "Coke-shard-story" but - indeed - I can add } } information as to the "knocked off } } table"-part.... } } } } I had always heared (personal informations from } } elder REICHERT-freaks and service people, from } } lectures/lecturers in the 70ies and 80ies as } } well as personally from H. Sitte himself) that } } the group around H. SITTE at REICHERT (now } } LEICA) in the late 1950ies/60ies was/were doing } } such experiments with {glass plates} .... which } } were thrown down to the floor... and one of them } } (it was Sitte himself, as he told me) picked up } } the shards evaluating the edges of "the most } } promising one" for sectioning purposes (who of } } that group had the "invention" to use glass as } } a} crude { knife (element) I unfortunately don't } } know. } } } } By the way, digging a little bit on this in } } Google, I found one hint on a document in a } } German data bank at } } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver–ffentlicht } } ("A simple ultramicrotome with thermal advance" published) } } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } } } Note:Ý {Naturwissenschaften} is the former title } } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } } Another source:Ý http://www.freepatentsonline.com/3828641.html: } } United States Patent US3828641: } } } } ==} Apparatus for adjusting the elevation of a } } specimen in microtomes, particularly } } ultramicrotomes } } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } } [as written in the patent document!]/Saar } } Germany) } } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } } Filed:Ý Nov. 10,1972 } } } } Also see } } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } } (From the book Michael J. Dykstra, Laura E. } } Reuss - 2003Ý (see page "History: in the text } } displayed } } the Reichert OMU-1 (before 1964, perhaps see } } document above in {Naturwissenschaften} 1955) } } from Sitte described as having thermal advance, } } Sitte was never happy with, since 1964: OMU-2 } } with thermal advance.... } } } } This just for your pleasure.... } } (and I sure that searching for Sitte Hellmuth or } } Sitte and microtome will find some results more } } on that interesting and exciting "history" of } } ultramicrotomy advances..... } } } } Best wishes and regards, } } have a good and successful rest of the week and a beautiful weekend, } } } } Wolfgang MUSS (Ph.D.) } } EM-Lab Gen.Hosp. SALK-LKH } } SALZBURG } } AUSTRIA } } } } } } } } } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } } Gesendet: Donnerstag, 20. J”nner 2011 06:51 } } } An: Muþ Wolfgang } } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } } America } } } ToÝ Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } It is certainly clear that there are many interesting stories --some } } } urban legends, and some insights into the minds of those that founded } } } the discipline of thin section electron microscopy. } } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } } microtome that I was told was developed by Sjostrand. } } } This thing consisted of a disk-like plate, about 4-5 inches in } } } diameter, with an eccentrically mounted arm that stuck out of the } } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } } The plate itself was mounted in a frame and lubricated with oil --the } } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } } on the wall by a long V belt (to minimize vibration), and the entire } } } plate rotated, bringing the block past the knife on each rotation.Ý The } } } sections that resulted tended to be arc shaped.Ý We got it working, but } } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } } remembers this type of machine -- assuming that my description is } } } comprehensible. } } } } } } The other microtome that I used, and really admire, was the Huxley } } } microtome, originally sold by Cambridge, and ultimately marketed by } } } LKB.Ý Although it used a mechanical advance, all of the movements of } } } the cutting arm were made by bending sheets of spring steel, so that } } } there were no surfaces that moved against each other.Ý Moreover, the } } } cutting stroke was controlled by an oil-filled damper, to minimize } } } chatter. -- a remarkable and stable machine. } } } } } } Now, the urban legend. } } } I had heard that a group (unnamed) was having a vigorous discussion } } } about the best way to strop a steel knife so as to get the best edge } } } for cutting thin sections.Ý How many strokes, in which direction, which } } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } } knocked off the table and broke into many pieces. } } } The story goes that one, or another, of these people took up one of the } } } shards, and suggested that it would make a good knife edge. } } } Verification anyone? } } } } } } Joel } } } } } } -- } } } Joel B. Sheffield, Ph.D. } } } Biology Department, Temple University } } } 1900 North 12th Street } } } Philadelphia, PA 19122 } } } jbs-at-temple.edu } } } (215) 204 8839, fax (215) 204 0486 } } } http://astro.temple.edu/~jbs } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } } ==============================Original Headers============================== } 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 } 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 } 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 } 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); } 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 } 6, 27 -- Mime-Version: 1.0 } 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} } 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 } 6, 27 -- To: Microscopy-at-microscopy.com } 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" } 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] } 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) } 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 } 6, 27 -- X-CanItPRO-Stream: default } 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 } 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 7, 35 -- From joelsheffield-at-gmail.com Thu Jan 20 11:31:19 2011 7, 35 -- Received: from mail-ww0-f41.google.com (mail-ww0-f41.google.com [74.125.82.41]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHVHTs032381 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:31:18 -0600 7, 35 -- Received: by wwi18 with SMTP id 18so1718115wwi.0 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:31:16 -0800 (PST) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 7, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 7, 35 -- bh=0t+PYP/uH2Mdg2F0J7RJ8voEOJFKxX7bczfl+eGG/Co=; 7, 35 -- b=cbjmwQQucf57+U/vclDM54yIhxJrPqlb7rnwS8YgK1VQ9rIgRheH1j2tzOjk0fpzZ/ 7, 35 -- HQVgzLPBASgodYlvxv7vYXdZQ8ElKg8dSosDGJoAyxiBSJgz/4p6tgqpPWB+33yz6bhj 7, 35 -- /VjCG95PXDhJ2NNRliP6JrVWIGXY83BIAqwaA= 7, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 7, 35 -- :content-type:content-transfer-encoding; 7, 35 -- b=ANU0y+3BwOi1bDDls16xlG64Hpm/SyR/j8iJyVxzEjzc65G9X1p76jnbH2/yyYbcHg 7, 35 -- S8J7OUwpvJyyYJiQM+0WgkzQF1vAyRiywAavPcKpfu8fwYBJhCLhcBQjPb27j2TQsVP+ 7, 35 -- cOw/cILd8E/daSyBM6ZFGyjKpC4QaiHr2m5vk= 7, 35 -- MIME-Version: 1.0 7, 35 -- Received: by 10.227.157.19 with SMTP id z19mr2626557wbw.189.1295544676290; 7, 35 -- Thu, 20 Jan 2011 09:31:16 -0800 (PST) 7, 35 -- Received: by 10.227.143.213 with HTTP; Thu, 20 Jan 2011 09:31:14 -0800 (PST) 7, 35 -- In-Reply-To: {201101201559.p0KFxBRa009241-at-ns.microscopy.com} 7, 35 -- References: {201101201559.p0KFxBRa009241-at-ns.microscopy.com} 7, 35 -- Date: Thu, 20 Jan 2011 12:31:14 -0500 7, 35 -- Message-ID: {AANLkTimynhsc+65Ai6DSg8gWQ=P6RaJZjn0ZWY7TB-og-at-mail.gmail.com} 7, 35 -- Subject: Re: [Microscopy] Re: Microtome History - was "Ernst Ruska" 7, 35 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 7, 35 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com 7, 35 -- Content-Type: text/plain; charset=windows-1252 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHVHTs032381 ==============================End of - Headers==============================
I can confirm the rubber mallet story for column alignment. I worked at Mellon Institute in Pittsburgh in the '70's. So did my father, Martin Haller, who taught me electron Microscopy. He worked there from the '60's to the '70's. We had several RCA electron microscopes that were purchased many years before I was trained in microscopy. My father used to align the lenses in the column of these microscopes with a rubber mallet. I took a couple of photos on these microscopes back in 1974. They shot glass plates with 3 exposures. After the 3 shots, you had to break vacuum, take out your plate, replace it and repump the scope to be able to take any more photos.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: PhillipsT-at-missouri.edu [PhillipsT-at-missouri.edu] Sent: Thursday, January 20, 2011 10:06 AM To: Haller, Edward
I have heard all the microtome and glass knives stories also but have no evidence to their veracity. But I once was talking to Sanford Palay - an early pioneer in EM - and he told me how they used a rubber hammer to tap the outside of the TEM column to try and nudge the aperture into place. I can't imagine how tedious that must have been. Of course, the reward the pioneers all got from primitive microtomy and TEMs was that they got to discover amazing new things of a bigger magnitude than most of the incremental advances we all get to contribute to these days. But I think I would rather have done science then rather than now when high cost forces specialization and a greater proportion of time is devoted to finding funding rather than organelles. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu] Sent: Thursday, January 20, 2011 8:20 AM To: Phillips, Thomas E.
Fellow microscopists:
I was taught decades ago that early ultrathin sections were made in the following manner:
A sheet of plate glass was dropped on the floor. The shard with the best edge was selected and attached to the blade of a small window fan. The resin-embedded specimen was attached to the tip of a soldering iron. once everything was clamped and aligned, the fan was turned on, the soldering iron was plugged in, and the section were collected in a large pan of water held beneath the fan. (As the soldering iron heated, the thermal expansion advanced the specimen into the glass blade.) Sections of optimal thickness were selected from those floating in the pan on the basis of the color refracted.
I love the story. Does anyone know whether this even approaches a true story?
Thanks,
Don
P.S. Anyone still alive who has first-hand knowledge of this story would be pretty old by now, but I thought that I would give this a shot.
--
Donald L. Lovett e-mail: lovett-at-tcnj.edu Professor and Chairperson phone: 609-771-2876 Department of Biology fax: 609-637-5118 The College of New Jersey P.O. Box 7718 Ewing, NJ 08628-0718
==============================Original Headers============================== 10, 28 -- From lovett-at-tcnj.edu Thu Jan 20 08:19:04 2011 10, 28 -- Received: from mailgate-too.TCNJ.EDU (mailgate-too.TCNJ.EDU [159.91.13.67]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KEJ4dB030331 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:19:04 -0600 10, 28 -- Received: from zcs.tcnj.edu (zcs.TCNJ.EDU [159.91.15.209]) 10, 28 -- by mailgate-too.TCNJ.EDU (Postfix) with ESMTP id 58885FF57E 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:13:11 -0500 (EST) 10, 28 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id CAF513373AB 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- X-Virus-Scanned: amavisd-new at zcs.TCNJ.EDU 10, 28 -- Received: from zcs.tcnj.edu ([127.0.0.1]) 10, 28 -- by localhost (zcs.tcnj.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 28 -- with ESMTP id fQjpUdFveSTN for {Microscopy-at-microscopy.com} ; 10, 28 -- Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Received: from [159.91.213.72] (unknown [159.91.213.72]) 10, 28 -- by zcs.tcnj.edu (Postfix) with ESMTP id AF779337393 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:19:02 -0500 (EST) 10, 28 -- Message-ID: {4D384456.6040204-at-tcnj.edu} 10, 28 -- Date: Thu, 20 Jan 2011 09:19:02 -0500 10, 28 -- From: "Donald L. Lovett" {lovett-at-tcnj.edu} 10, 28 -- Organization: Department of Biology, The College of New Jersey 10, 28 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 10, 28 -- MIME-Version: 1.0 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Subject: Early microtomy--urban legend? 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 32 -- From PhillipsT-at-missouri.edu Thu Jan 20 08:54:00 2011 19, 32 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KErxrG030207 19, 32 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:54:00 -0600 19, 32 -- X-IronPort-Anti-Spam-Filtered: true 19, 32 -- X-IronPort-Anti-Spam-Result: AvsEAN7aN03RauUo/2dsb2JhbACkUXO1cYhEAoMQgj4EhG+BN4Qsg3Y 19, 32 -- Received: from um-tsmtpout1.um.umsystem.edu ([209.106.229.40]) 19, 32 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-tsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 19, 32 -- Thu, 20 Jan 2011 08:53:59 -0600 19, 32 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 19, 32 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, 20 Jan 2011 19, 32 -- 08:53:58 -0600 19, 32 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 19, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 32 -- Date: Thu, 20 Jan 2011 08:53:54 -0600 19, 32 -- Subject: RE: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Topic: [Microscopy] Early microtomy--urban legend? 19, 32 -- Thread-Index: Acu4rTCfGg2oH1rLRqikoaKr1g1gsgAA+QUQ 19, 32 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF1A-at-UM-EMAIL05.um.umsystem.edu} 19, 32 -- References: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- In-Reply-To: {201101201420.p0KEKJfH031321-at-ns.microscopy.com} 19, 32 -- Accept-Language: en-US 19, 32 -- Content-Language: en-US 19, 32 -- X-MS-Has-Attach: 19, 32 -- X-MS-TNEF-Correlator: 19, 32 -- acceptlanguage: en-US 19, 32 -- Content-Type: text/plain; charset="us-ascii" 19, 32 -- MIME-Version: 1.0 19, 32 -- X-OriginalArrivalTime: 20 Jan 2011 14:53:59.0017 (UTC) FILETIME=[DE667590:01CBB8B1] 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KErxrG030207 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 37 -- From ehaller-at-health.usf.edu Thu Jan 20 11:43:12 2011 26, 37 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 26, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHhCcS016439 26, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:43:12 -0600 26, 37 -- X-ASG-Debug-ID: 1295545391-2cee01ca0000-4CH8be 26, 37 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-bin/mark.cgi 26, 37 -- Received: from mailhub1.hscnet.hsc.usf.edu (localhost [127.0.0.1]) 26, 37 -- by hscantispam.health.usf.edu (Spam & Virus Firewall) with ESMTP 26, 37 -- id 192D7790BEDE; Thu, 20 Jan 2011 12:43:11 -0500 (EST) 26, 37 -- Received: from mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) by hscantispam.health.usf.edu with ESMTP id vWDQSRI9gyDKBfzE; Thu, 20 Jan 2011 12:43:11 -0500 (EST) 26, 37 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 26, 37 -- Received: from MAILSERVER3.hscnet.hsc.usf.edu ([10.119.4.19]) by 26, 37 -- mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) with mapi; Thu, 20 Jan 2011 26, 37 -- 12:43:11 -0500 26, 37 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 26, 37 -- To: "PhillipsT-at-missouri.edu" {PhillipsT-at-missouri.edu} , 26, 37 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 26, 37 -- Date: Thu, 20 Jan 2011 12:43:11 -0500 26, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: Early microtomy--urban legend? 26, 37 -- Subject: RE: [Microscopy] RE: Early microtomy--urban legend? 26, 37 -- Thread-Topic: [Microscopy] RE: Early microtomy--urban legend? 26, 37 -- Thread-Index: Acu4s4/fp3dncIDRQdqcJkxODIBNBwAFS1Bc 26, 37 -- Message-ID: {5B34016F48340D428A2644D479F587FC359C34E3-at-MAILSERVER3.hscnet.hsc.usf.edu} 26, 37 -- References: {201101201506.p0KF64df001518-at-ns.microscopy.com} 26, 37 -- In-Reply-To: {201101201506.p0KF64df001518-at-ns.microscopy.com} 26, 37 -- Accept-Language: en-US 26, 37 -- Content-Language: en-US 26, 37 -- X-MS-Has-Attach: 26, 37 -- X-MS-TNEF-Correlator: 26, 37 -- acceptlanguage: en-US 26, 37 -- Content-Type: text/plain; charset="us-ascii" 26, 37 -- MIME-Version: 1.0 26, 37 -- X-Barracuda-Connect: UNKNOWN[10.119.4.12] 26, 37 -- X-Barracuda-Start-Time: 1295545392 26, 37 -- X-Barracuda-Virus-Scanned: by USF Health AntiSpam System at health.usf.edu 26, 37 -- Content-Transfer-Encoding: 8bit 26, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHhCcS016439 ==============================End of - Headers==============================
Dear All, I had the same problem visualizing bee wax structures and not owning an ESEM ;-)
Solution: I put the structure on a large specimen mount, putting 2 bands of conductive adhesive tape left and right on top and put it in my normal Pt-sputtercoater. Concerning the heat during sputtering, I took off the sputter-head and added a second glass vessel (I took it from my carbon-coater) on top of the first. Did ca. 10-15 sputtering-runs with minimal current (10mA) and finally put it in the scope at 5-6kV.
See images at http://www.electronmicroscopy.info/wax
It`s a bit of a crude solution but it works. Concerning the thickness of your wax layers you may also try the same thing with an high-vac chromecoater...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Dear Zhou, If possible, I would put in a very small SA aperture and try to use a parallel beam to get Bragg spots. These precipitates are very small, so you may need to take long exposures, which means that the specimen and aperture positions must be very stable. In order to see diffraction from the small particles, the diffraction from the much larger surrounding area must be minimized (or subtracted out). Yours, Bill
X-from: z.zhou-at-lboro.ac.uk To: William.F.Tivol-at-aero.org
Organization: Dept of Materials, Loughborough University, UK
Title-Subject: [Filtered] TEM diffraction 3nm precipitates
Message: Greetings listers,
Iím studying crystal structure of some carbides/nitrides precipitates in steel. The precipitates are 2-5nm size in a C replica TEM specimen, V-, Cr-, and Nb-rich by STEM/EDX.
How can I get electron diffraction or crystal structure information of these fine precipitates?
I tried on a TecnaiF20 TEM using nanoprobe spot 7, focused on a small particle, then diffraction, most of the time I got amorphous rings of C support, no obvious diffraction discs. STEM mode did indicate some C contamination on the session. Assuming minimal C contamination, is it possible that this method can give me some sort of convergence beam electron diffraction pattern, which may help me determine the crystal structure of the precipitates?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KIUVdS001596 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 20 Jan 2011 12:30:31 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p0KIUVur001594; Thu, 20 Jan 2011 12:30:31 -0600
Dear Frank, The key is that you're "looking for slight changes in element concentrations at grain boundaries and precipitates." In order best to see these changes, the pixel size should be smaller than the features--half the feature size guarantees that at least one pixel will be entirely within the feature. If the pixel size is too large than you will measure the average element concentrations over both the feature and the surrounding area, which will make the apparent changes smaller than they really are. I would suggest taking an image to locate the features, then place the beam on each feature in turn to do the analysis. Also take a spectrum of the area next to each feature. This should give you the best chance of seeing differences and minimize the amount of data. Yours, Bill
X-from: Frank_Karl-at-lincolnelectric.com To: William.F.Tivol-at-aero.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Iâ??ve been thinking about EDS line scans and Iâ??m attempting to maximize the data I collect.
I run at 20 KV on iron samples so my electron interaction range is between 1.5 to 2.3um (Berthe or K-O range calculations). According to data provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at 700x. So right away I see if each image pixel represents a data point, Iâ??m sampling about 2 pixel volumes per data point. Looking for a sudden and sharp change in element concentration seem difficult, but wait thereâ??s more. Let us introduce another plot complication.
My customers donâ??t want to scan the entire image, tooo much dataâ?¦
So I scan a smaller section of image described above and instruct the computer to take 512 data points along a line that might only be 400 (visual estimate from screen) image pixels long.
Soâ?¦ Should I reduce the image resolution so that each image pixel will be one data point and scan the entire image? Would it be better to lower the magnification so the image pixels are larger (say 1.2um ). I could go to 100x and 512 horizontal image pixel so each image pixel is 2.3um?
Iâ??m looking for slight changes in element concentration at grain boundaries and precipitates and of course I want to produce the most meaningful data possible. Iâ??m running at conditions that suggest my electron interactive volume is larger than my spatial resolution as to produce â??continuousâ? data.
Or am I just over thinking the process?
Any suggestion or comments would be welcome
Frank Lincoln Electric Company -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 17 -- From frank_karl-at-lincolnelectric.com Wed Jan 19 07:37:23 2011 11, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDbNoO031503 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:37:23 -0600 11, 17 -- Subject: EDS line scans and time on my hands 11, 17 -- To: Microscopy-at-microscopy.com 11, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 17 -- Message-ID: {OF4A811DE5.D47D1408-ON8525781D.004A569D-8525781D.004AD372-at-lincolnelectric.com} 11, 17 -- From: Frank_Karl-at-lincolnelectric.com 11, 17 -- Date: Wed, 19 Jan 2011 08:38:03 -0500 11, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 11, 17 -- 01/19/2011 08:37:18 AM 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; 11, 17 -- charset="UTF-8" 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0JDbNoO031503 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KIXB2C006837 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 20 Jan 2011 12:33:11 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p0KIXB6F006835; Thu, 20 Jan 2011 12:33:11 -0600
This sounds more like a food processor than a microtome!
John Mardinly
Western Digital
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Thursday, January 20, 2011 8:30 AM To: John Mardinly
I just remembered seeing a print ad from some science journal published the 40's or 50's (before my time!) for an early ultramicrotome. It was a high speed motor spinning some type of blade. The concept was that the block was advanced into this buzzsaw and you were supposed to catch the sections flying off. At the time I think the view was that ultrathins could only be cut at high speed. The real kicker was the ad mentioned the motor was also suitable for use in centrifuges. Crazy. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Thursday, January 20, 2011 9:54 AM To: Phillips, Thomas E.
I know at least 2 old microscopists who made glass knifes by breaking window glass - one of them here at CMU. He went to construction sites to get the broken windows.
I'm running down the reference and (I hope) an image, but one of the early tries at ultramicrotomy was sticking razor blades on a centrifuge rotor, then mount the sections on the tub, close the lid and turn on the centrifuge. The sections where then picked up from inside the tub, having been flung willy-nilly around the inside. (The image is used in the microtomy lecture to convince the students thin sectioning could be a lot worse than they think it is.)
Phil
} Von: Mu Wolfgang } Gesendet: Donnerstag, 20. J"nner 2011 11:46 } An: 'joelsheffield-at-gmail.com' } Cc: 'microscopy-at-microscopy.com' } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } Joel wrote: } } Now, the urban legend. } I had heard that a group (unnamed) was having a } vigorous discussion about the best way to strop } a steel knife so as to get the best edge for } cutting thin sections. How many strokes, in } which direction, which side of the blade, etc. } During the discussion, a bottle of Coke was } knocked off the table and broke into many } pieces. } The story goes that one, or another, of these } people took up one of the shards, and suggested } that it would make a good knife edge. } Verification anyone? { } } Joel } } } Good morning, } Dear Joel, dear all } } I can't tell or verify the above mentioned } "Coke-shard-story" but - indeed - I can add } information as to the "knocked off } table"-part.... } } I had always heared (personal informations from } elder REICHERT-freaks and service people, from } lectures/lecturers in the 70ies and 80ies as } well as personally from H. Sitte himself) that } the group around H. SITTE at REICHERT (now } LEICA) in the late 1950ies/60ies was/were doing } such experiments with {glass plates} .... which } were thrown down to the floor... and one of them } (it was Sitte himself, as he told me) picked up } the shards evaluating the edges of "the most } promising one" for sectioning purposes (who of } that group had the "invention" to use glass as } a} crude { knife (element) I unfortunately don't } know. } } By the way, digging a little bit on this in } Google, I found one hint on a document in a } German data bank at } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H. } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht } ("A simple ultramicrotome with thermal advance" published) } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } Note: {Naturwissenschaften} is the former title } of Biomedical and Life Sciences (SPRINGER) } Another source: http://www.freepatentsonline.com/3828641.html: } United States Patent US3828641: } } ==} Apparatus for adjusting the elevation of a } specimen in microtomes, particularly } ultramicrotomes } Inventor: Hellmuth Sitte, HOMBURG (not Humburg } [as written in the patent document!]/Saar } Germany) } Assignee: C.Reichert Optische Werke AG, Vienna, Austria) } Filed: Nov. 10,1972 } } Also see } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+an d+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl =de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CC EQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } (From the book Michael J. Dykstra, Laura E. } Reuss - 2003 (see page "History: in the text } displayed } the Reichert OMU-1 (before 1964, perhaps see } document above in {Naturwissenschaften} 1955) } from Sitte described as having thermal advance, } Sitte was never happy with, since 1964: OMU-2 } with thermal advance.... } } This just for your pleasure.... } (and I sure that searching for Sitte Hellmuth or } Sitte and microtome will find some results more } on that interesting and exciting "history" of } ultramicrotomy advances..... } } Best wishes and regards, } have a good and successful rest of the week and a beautiful weekend, } } Wolfgang MUSS (Ph.D.) } EM-Lab Gen.Hosp. SALK-LKH } SALZBURG } AUSTRIA } } } } } } } } } -----Ursprngliche Nachricht----- } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } Gesendet: Donnerstag, 20. J"nner 2011 06:51 } } An: Mu Wolfgang } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } ----------------------------------------------------------------------- } } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ----------------------------------------------------------------------- } } ----- } } } } It is certainly clear that there are many interesting stories --some } } urban legends, and some insights into the minds of those that founded } } the discipline of thin section electron microscopy. } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } microtome that I was told was developed by Sjostrand. } } This thing consisted of a disk-like plate, about 4-5 inches in } } diameter, with an eccentrically mounted arm that stuck out of the } } plate. The arm had a chuck for a tissue sample, and a heating coil. } } The plate itself was mounted in a frame and lubricated with oil --the } } plate acted like a large oil bearing. The plate was linked to a motor } } on the wall by a long V belt (to minimize vibration), and the entire } } plate rotated, bringing the block past the knife on each rotation. The } } sections that resulted tended to be arc shaped. We got it working, but } } it was a terrifying thing to see. I wonder if anyone in this group } } remembers this type of machine -- assuming that my description is } } comprehensible. } } } } The other microtome that I used, and really admire, was the Huxley } } microtome, originally sold by Cambridge, and ultimately marketed by } } LKB. Although it used a mechanical advance, all of the movements of } } the cutting arm were made by bending sheets of spring steel, so that } } there were no surfaces that moved against each other. Moreover, the } } cutting stroke was controlled by an oil-filled damper, to minimize } } chatter. -- a remarkable and stable machine. } } } } Now, the urban legend. } } I had heard that a group (unnamed) was having a vigorous discussion } } about the best way to strop a steel knife so as to get the best edge } } for cutting thin sections. How many strokes, in which direction, which } } side of the blade, etc. During the discussion, a bottle of Coke was } } knocked off the table and broke into many pieces. } } The story goes that one, or another, of these people took up one of the } } shards, and suggested that it would make a good knife edge. } } Verification anyone? } } } } Joel } } } } -- } } Joel B. Sheffield, Ph.D. } } Biology Department, Temple University } } 1900 North 12th Street } } Philadelphia, PA 19122 } } jbs-at-temple.edu } } (215) 204 8839, fax (215) 204 0486 } } http://astro.temple.edu/~jbs
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 6, 27 -- Mime-Version: 1.0 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 6, 27 -- To: Microscopy-at-microscopy.com 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 35 -- From PhillipsT-at-missouri.edu Thu Jan 20 10:20:18 2011 16, 35 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 16, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KGKG9E014299 16, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:20:16 -0600 16, 35 -- X-IronPort-Anti-Spam-Filtered: true 16, 35 -- X-IronPort-Anti-Spam-Result: AvsEAH7vN03RauUp/2dsb2JhbACkUXO2AYheAoJuIoI+BIRviVmCcw 16, 35 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.229.41]) 16, 35 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 20 Jan 2011 10:20:14 -0600 16, 35 -- Received: from UM-THUB01.um.umsystem.edu ([209.106.230.181]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 16, 35 -- Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from UM-MSXHUB05.um.umsystem.edu (131.151.121.205) by 16, 35 -- UM-THUB01.um.umsystem.edu (209.106.230.181) with Microsoft SMTP Server (TLS) 16, 35 -- id 8.2.254.0; Thu, 20 Jan 2011 10:19:55 -0600 16, 35 -- Received: from um-email05.um.umsystem.edu ([169.254.1.36]) by 16, 35 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Thu, 20 Jan 2011 16, 35 -- 10:19:54 -0600 16, 35 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 35 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 35 -- Date: Thu, 20 Jan 2011 10:19:53 -0600 16, 35 -- Subject: [Microscopy] Re: Microtome History 16, 35 -- Thread-Topic: [Microscopy] Re: Microtome History 16, 35 -- Thread-Index: Acu4ujnK/W+eYZMgQe2m/DmfMJG5qAAAyCyg 16, 35 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF1C4596EF46-at-UM-EMAIL05.um.umsystem.edu} 16, 35 -- References: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- In-Reply-To: {201101201553.p0KFrlKj031360-at-ns.microscopy.com} 16, 35 -- Accept-Language: en-US 16, 35 -- Content-Language: en-US 16, 35 -- X-MS-Has-Attach: 16, 35 -- X-MS-TNEF-Correlator: 16, 35 -- acceptlanguage: en-US 16, 35 -- Content-Type: text/plain; charset="iso-8859-1" 16, 35 -- MIME-Version: 1.0 16, 35 -- X-OriginalArrivalTime: 20 Jan 2011 16:19:55.0726 (UTC) FILETIME=[E009CEE0:01CBB8BD] 16, 35 -- Content-Transfer-Encoding: 8bit 16, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KGKG9E014299 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 30 -- From prvs=994c3f914=John.Mardinly-at-wdc.com Thu Jan 20 12:33:11 2011 26, 30 -- Received: from wdscspam2.wdc.com (wdscspam2.wdc.com [129.253.55.43]) 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KIX9hP006832 26, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 12:33:10 -0600 26, 30 -- X-IronPort-AV: E=Sophos;i="4.60,352,1291622400"; 26, 30 -- d="scan'208";a="172514723" 26, 30 -- Received: from unknown (HELO wdscexfe01.sc.wdc.com) ([172.25.19.1]) 26, 30 -- by wdscspam2.wdc.com with ESMTP; 20 Jan 2011 10:33:04 -0800 26, 30 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.4675); 26, 30 -- Thu, 20 Jan 2011 10:33:09 -0800 26, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 30 -- Content-class: urn:content-classes:message 26, 30 -- MIME-Version: 1.0 26, 30 -- Content-Type: text/plain; 26, 30 -- charset="us-ascii" 26, 30 -- Subject: RE: [Microscopy] Re: Microtome History 26, 30 -- Date: Thu, 20 Jan 2011 10:33:08 -0800 26, 30 -- Message-ID: {34061960C62E274C8AF1DCAA65655558097AA6D8-at-wdksjexbe01.msj.wdc.com} 26, 30 -- In-Reply-To: {201101201630.p0KGU7CT027262-at-ns.microscopy.com} 26, 30 -- X-MS-Has-Attach: 26, 30 -- X-MS-TNEF-Correlator: 26, 30 -- Thread-Topic: [Microscopy] Re: Microtome History 26, 30 -- Thread-Index: Acu4v1BKcpZtax5uRhqiCEWvlx7rtAAEQtjQ 26, 30 -- References: {201101201630.p0KGU7CT027262-at-ns.microscopy.com} 26, 30 -- From: "John Mardinly" {John.Mardinly-at-wdc.com} 26, 30 -- To: {PhillipsT-at-missouri.edu} 26, 30 -- Cc: {Microscopy-at-microscopy.com} 26, 30 -- X-OriginalArrivalTime: 20 Jan 2011 18:33:09.0168 (UTC) FILETIME=[7C804300:01CBB8D0] 26, 30 -- Content-Transfer-Encoding: 8bit 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KIX9hP006832 ==============================End of - Headers==============================
One of the hospitals here in Honolulu had an old RCA TEM that had a rubber mallet in the alignment kit. They kept that old scope going until the early 1980s, I believe, with frequent visits from an aged service technician. It was a mechanical wonder.
I also heard of many of the early iterations of ultramicrotome, possibly at the knee of Caroline Schooley, from whom I learned TEM in 1976. One version I heard was mounting a block on the blade of a fan and running it past a stationary knife at high speed, using the position of venetian blinds on a sunny day as thickness control. One had to search around the room for the sections. I had to make knives from plate glass for a couple of years, and I might as well have given up on the pliers and just thrown the glass on the floor...
Aloha, Tina
Twenty to thirty foot waves predicted on the North Shore today.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 22 -- From tina-at-pbrc.hawaii.edu Thu Jan 20 12:34:02 2011 6, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KIY0GB009168 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 12:34:00 -0600 6, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p0KIXwu7025021 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:33:58 -1000 (HST) 6, 22 -- Received: from localhost (tina-at-localhost) 6, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p0KIXvLm025016 6, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 08:33:58 -1000 (HST) 6, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 6, 22 -- Date: Thu, 20 Jan 2011 08:33:57 -1000 (HST) 6, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 22 -- X-X-Sender: tina-at-b1000 6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] Early microtomy--urban legend? 6, 22 -- In-Reply-To: {201101201744.p0KHi2wr017777-at-ns.microscopy.com} 6, 22 -- Message-ID: {Pine.GSO.4.64.1101200823400.24710-at-b1000} 6, 22 -- References: {201101201744.p0KHi2wr017777-at-ns.microscopy.com} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
If you check out the Glauert texts, in the volume by norma Reid and julian Beesley (pub 1991), they talk about the old high speed microtomes apparently razor blades were rotated at 10k rpm (the refs are Obrien et al 1943, and Fullam et al 1946, the Obrien paper was actually in Science vol 98, 455-456). For fun I made a similar device out of a dremel and a plastic salad bowl, the dremel cut the sections which flew off and stuck to the inner surface of the plastic bowl. I then filled it with water and the sections (at least some of them) floated off. The thickness was completely random, and the bowl size a bit enormous and not stationary.. think collecting sections from the surface of the Atlantic... but they were useable.. sort of... Obviously no serial sections. The other thing is that I was using contemporary resin technology, not available 65 years ago when this work was done, but I guess at the "cutting edge" (pun intended) S
Simon C. Watkins Ph.D, FRC Path Professor and Vice Chair Cell Biology and Physiology Professor Immunology Director Center for Biologic Imaging BSTS 225 University of Pittsburgh 3500 Terrace St Pittsburgh PA 15261 412-352-2277 www.cbi.pitt.edu
-----Original Message----- X-from: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] Sent: Thursday, January 20, 2011 12:37 PM To: Watkins, Simon C
Indeed. We are in a building that was often broken into. Years ago, I made it my business to collect the old door panes --they were a tinted glass, about 1/4" thick, and had just the right temper to make excellent knives.
Joel
On Thu, Jan 20, 2011 at 10:59 AM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I know at least 2 old microscopists who made } glass knifes by breaking window glass - one of } them here at CMU. He went to construction sites } to get the broken windows. } } I'm running down the reference and (I hope) an } image, but one of the early tries at } ultramicrotomy was sticking razor blades on a } centrifuge rotor, then mount the sections on the } tub, close the lid and turn on the centrifuge. } The sections where then picked up from inside the } tub, having been flung willy-nilly around the } inside. } (The image is used in the microtomy lecture to } convince the students thin sectioning could be a } lot worse than they think it is.) } } Phil } } } Von: Muþ Wolfgang } } Gesendet: Donnerstag, 20. J"nner 2011 11:46 } } An: 'joelsheffield-at-gmail.com' } } Cc: 'microscopy-at-microscopy.com' } } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } Joel wrote: } } } Now, the urban legend.Ý } } I had heard that a group (unnamed) was having a } } vigorous discussion about the best way to strop } } a steel knife so as to get the best edge for } } cutting thin sections.Ý How many strokes, in } } which direction, which side of the blade, etc.Ý } } During the discussion, a bottle of Coke was } } knocked off the table and broke into many } } pieces.Ý } } The story goes that one, or another, of these } } people took up one of the shards, and suggested } } that it would make a good knife edge. } } Verification anyone? { } } } } Joel } } } } } } Good morning, } } Dear Joel, dear all } } } } I can't tell or verify the above mentioned } } "Coke-shard-story" but - indeed - I can add } } information as to the "knocked off } } table"-part.... } } } } I had always heared (personal informations from } } elder REICHERT-freaks and service people, from } } lectures/lecturers in the 70ies and 80ies as } } well as personally from H. Sitte himself) that } } the group around H. SITTE at REICHERT (now } } LEICA) in the late 1950ies/60ies was/were doing } } such experiments with {glass plates} .... which } } were thrown down to the floor... and one of them } } (it was Sitte himself, as he told me) picked up } } the shards evaluating the edges of "the most } } promising one" for sectioning purposes (who of } } that group had the "invention" to use glass as } } a} crude { knife (element) I unfortunately don't } } know. } } } } By the way, digging a little bit on this in } } Google, I found one hint on a document in a } } German data bank at } } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht } } ("A simple ultramicrotome with thermal advance" published) } } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } } } Note:Ý {Naturwissenschaften} is the former title } } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } } Another source:Ý http://www.freepatentsonline.com/3828641.html: } } United States Patent US3828641: } } } } ==} Apparatus for adjusting the elevation of a } } specimen in microtomes, particularly } } ultramicrotomes } } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } } [as written in the patent document!]/Saar } } Germany) } } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } } Filed:Ý Nov. 10,1972 } } } } Also see } } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false } } (From the book Michael J. Dykstra, Laura E. } } Reuss - 2003Ý (see page "History: in the text } } displayed } } the Reichert OMU-1 (before 1964, perhaps see } } document above in {Naturwissenschaften} 1955) } } from Sitte described as having thermal advance, } } Sitte was never happy with, since 1964: OMU-2 } } with thermal advance.... } } } } This just for your pleasure.... } } (and I sure that searching for Sitte Hellmuth or } } Sitte and microtome will find some results more } } on that interesting and exciting "history" of } } ultramicrotomy advances..... } } } } Best wishes and regards, } } have a good and successful rest of the week and a beautiful weekend, } } } } Wolfgang MUSS (Ph.D.) } } EM-Lab Gen.Hosp. SALK-LKH } } SALZBURG } } AUSTRIA } } } } } } } } } } } } } } } } } -----Urspr¸ngliche Nachricht----- } } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } } Gesendet: Donnerstag, 20. J"nner 2011 06:51 } } } An: Muþ Wolfgang } } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } } America } } } ToÝ Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } It is certainly clear that there are many interesting stories --some } } } urban legends, and some insights into the minds of those that founded } } } the discipline of thin section electron microscopy. } } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } } microtome that I was told was developed by Sjostrand. } } } This thing consisted of a disk-like plate, about 4-5 inches in } } } diameter, with an eccentrically mounted arm that stuck out of the } } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } } The plate itself was mounted in a frame and lubricated with oil --the } } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } } on the wall by a long V belt (to minimize vibration), and the entire } } } plate rotated, bringing the block past the knife on each rotation.Ý The } } } sections that resulted tended to be arc shaped.Ý We got it working, but } } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } } remembers this type of machine -- assuming that my description is } } } comprehensible. } } } } } } The other microtome that I used, and really admire, was the Huxley } } } microtome, originally sold by Cambridge, and ultimately marketed by } } } LKB.Ý Although it used a mechanical advance, all of the movements of } } } the cutting arm were made by bending sheets of spring steel, so that } } } there were no surfaces that moved against each other.Ý Moreover, the } } } cutting stroke was controlled by an oil-filled damper, to minimize } } } chatter. -- a remarkable and stable machine. } } } } } } Now, the urban legend. } } } I had heard that a group (unnamed) was having a vigorous discussion } } } about the best way to strop a steel knife so as to get the best edge } } } for cutting thin sections.Ý How many strokes, in which direction, which } } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } } knocked off the table and broke into many pieces. } } } The story goes that one, or another, of these people took up one of the } } } shards, and suggested that it would make a good knife edge. } } } Verification anyone? } } } } } } Joel } } } } } } -- } } } Joel B. Sheffield, Ph.D. } } } Biology Department, Temple University } } } 1900 North 12th Street } } } Philadelphia, PA 19122 } } } jbs-at-temple.edu } } } (215) 204 8839, fax (215) 204 0486 } } } http://astro.temple.edu/~jbs } } -- } Philip Oshel } Microscopy Facility Supervisor } Biology Department } 024C Brooks Hall } Central Michigan University } Mt. Pleasant, MI 48859 } (989) 774-3576 } } } ==============================Original Headers============================== } 6, 27 -- From oshel1pe-at-cmich.edu Thu Jan 20 09:53:21 2011 } 6, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KFrLZ3030383 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:53:21 -0600 } 6, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 6, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p0KFrBHA005464 } 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 10:53:20 -0500 } 6, 27 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); } 6, 27 -- Thu, 20 Jan 2011 10:53:17 -0500 } 6, 27 -- Mime-Version: 1.0 } 6, 27 -- Message-Id: {a06240802c95e09c0c794-at-[141.209.160.249]} } 6, 27 -- In-Reply-To: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- References: {201101201050.p0KAoqC2029958-at-ns.microscopy.com} } 6, 27 -- Date: Thu, 20 Jan 2011 10:53:12 -0500 } 6, 27 -- To: Microscopy-at-microscopy.com } 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 6, 27 -- Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska" } 6, 27 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 6, 27 -- X-OriginalArrivalTime: 20 Jan 2011 15:53:17.0197 (UTC) FILETIME=[273DA3D0:01CBB8BA] } 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) } 6, 27 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,MIME_QP_LONG_LINE,RDNS_NONE,Bayes(0.0001:-0.5) } 6, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 } 6, 27 -- X-CanItPRO-Stream: default } 6, 27 -- X-Canit-Stats-ID: 02DVPRkyE - 9b55b8297af8 - 20110120 } 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KFrLZ3030383 } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 7, 35 -- From joelsheffield-at-gmail.com Thu Jan 20 11:31:19 2011 7, 35 -- Received: from mail-ww0-f41.google.com (mail-ww0-f41.google.com [74.125.82.41]) 7, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KHVHTs032381 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 11:31:18 -0600 7, 35 -- Received: by wwi18 with SMTP id 18so1718115wwi.0 7, 35 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 09:31:16 -0800 (PST) 7, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 7, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 7, 35 -- bh=0t+PYP/uH2Mdg2F0J7RJ8voEOJFKxX7bczfl+eGG/Co=; 7, 35 -- b=cbjmwQQucf57+U/vclDM54yIhxJrPqlb7rnwS8YgK1VQ9rIgRheH1j2tzOjk0fpzZ/ 7, 35 -- HQVgzLPBASgodYlvxv7vYXdZQ8ElKg8dSosDGJoAyxiBSJgz/4p6tgqpPWB+33yz6bhj 7, 35 -- /VjCG95PXDhJ2NNRliP6JrVWIGXY83BIAqwaA= 7, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 35 -- d=gmail.com; s=gamma; 7, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 7, 35 -- :content-type:content-transfer-encoding; 7, 35 -- b=ANU0y+3BwOi1bDDls16xlG64Hpm/SyR/j8iJyVxzEjzc65G9X1p76jnbH2/yyYbcHg 7, 35 -- S8J7OUwpvJyyYJiQM+0WgkzQF1vAyRiywAavPcKpfu8fwYBJhCLhcBQjPb27j2TQsVP+ 7, 35 -- cOw/cILd8E/daSyBM6ZFGyjKpC4QaiHr2m5vk= 7, 35 -- MIME-Version: 1.0 7, 35 -- Received: by 10.227.157.19 with SMTP id z19mr2626557wbw.189.1295544676290; 7, 35 -- Thu, 20 Jan 2011 09:31:16 -0800 (PST) 7, 35 -- Received: by 10.227.143.213 with HTTP; Thu, 20 Jan 2011 09:31:14 -0800 (PST) 7, 35 -- In-Reply-To: {201101201559.p0KFxBRa009241-at-ns.microscopy.com} 7, 35 -- References: {201101201559.p0KFxBRa009241-at-ns.microscopy.com} 7, 35 -- Date: Thu, 20 Jan 2011 12:31:14 -0500 7, 35 -- Message-ID: {AANLkTimynhsc+65Ai6DSg8gWQ=P6RaJZjn0ZWY7TB-og-at-mail.gmail.com} 7, 35 -- Subject: Re: [Microscopy] Re: Microtome History - was "Ernst Ruska" 7, 35 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 7, 35 -- To: oshel1pe-at-cmich.edu, microscopy-at-microscopy.com 7, 35 -- Content-Type: text/plain; charset=windows-1252 7, 35 -- Content-Transfer-Encoding: 8bit 7, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KHVHTs032381 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From swatkins-at-pitt.edu Thu Jan 20 13:01:00 2011 17, 27 -- Received: from exprod7og117.obsmtp.com (exprod7og117.obsmtp.com [64.18.2.6]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p0KJ0xnS017907 17, 27 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 13:00:59 -0600 17, 27 -- Received: from source ([136.142.251.42]) (using TLSv1) by exprod7ob117.postini.com ([64.18.6.12]) with SMTP 17, 27 -- ID DSNKTTiGao0K4p6etfxldeCwf8x0pHNvrjEz-at-postini.com; Thu, 20 Jan 2011 11:00:59 PST 17, 27 -- Received: from pitt-exch-14.univ.pitt.edu ([169.254.1.248]) by 17, 27 -- pitt-ht-04.univ.pitt.edu ([136.142.251.42]) with mapi; Thu, 20 Jan 2011 17, 27 -- 14:00:57 -0500 17, 27 -- From: "Watkins, Simon C" {swatkins-at-pitt.edu} 17, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 27 -- Date: Thu, 20 Jan 2011 14:00:48 -0500 17, 27 -- Subject: RE: [Microscopy] Microtome History - was "Ernst Ruska" 17, 27 -- Thread-Topic: [Microscopy] Microtome History - was "Ernst Ruska" 17, 27 -- Thread-Index: Acu4yLELPf6IttVpQ0yllQgruK+LSwACk0SQ 17, 27 -- Message-ID: {F53DA84501B039439893BE88F0F963410FFCAB6D8D-at-PITT-EXCH-14.univ.pitt.edu} 17, 27 -- References: {201101201737.p0KHbH2S007671-at-ns.microscopy.com} 17, 27 -- In-Reply-To: {201101201737.p0KHbH2S007671-at-ns.microscopy.com} 17, 27 -- Accept-Language: en-US 17, 27 -- Content-Language: en-US 17, 27 -- X-MS-Has-Attach: 17, 27 -- X-MS-TNEF-Correlator: 17, 27 -- acceptlanguage: en-US 17, 27 -- Content-Type: text/plain; charset="iso-8859-1" 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Transfer-Encoding: 8bit 17, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KJ0xnS017907 ==============================End of - Headers==============================
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This whole discussion has been a "this is my life" for me. Sorry, Tina - the fan thing wasn't mine. I went to work for Dorothy Pitelka at U.C. Berkeley in '53, and our first attempts at thin sections were with a metal-knife Spencer microtome with a modifying wedge in the advance mechanism. Miserable. Then a MT-1 with glass knives; a local window company made 1" strips from old windows for us.
The rubber mallet was used on RCA EMU-2 scopes to align the objective aperture....
Caroline -- Caroline Schooley Project MICRO Coordinator Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/ProjectMICRO
==============================Original Headers============================== 4, 18 -- From schooley-at-mcn.org Thu Jan 20 13:05:12 2011 4, 18 -- Received: from smtp2.mcn.org (smtp2.mcn.org [216.150.240.86]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KJ5Bml023547 4, 18 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 13:05:12 -0600 4, 18 -- Received: from [66.81.51.194] 4, 18 -- by smtp2.mcn.org with esmtpa (Exim 4.63) 4, 18 -- (envelope-from {schooley-at-mcn.org} ) 4, 18 -- id 1Pfzoi-0005EJ-6P; Thu, 20 Jan 2011 11:05:09 -0800 4, 18 -- Mime-Version: 1.0 4, 18 -- Message-Id: {a06200700c95e34089b6e-at-[66.81.41.32]} 4, 18 -- In-Reply-To: {201101201842.p0KIg3QP006164-at-ns.microscopy.com} 4, 18 -- References: {201101201842.p0KIg3QP006164-at-ns.microscopy.com} 4, 18 -- Date: Thu, 20 Jan 2011 11:05:00 -0800 4, 18 -- To: tina-at-pbrc.hawaii.edu 4, 18 -- From: Caroline Schooley {schooley-at-mcn.org} 4, 18 -- Subject: [Microscopy] Re: Early microtomy--urban legend? 4, 18 -- Cc: {microscopy-at-microscopy.com} 4, 18 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Paul Hazelton's comments about knowing someone who claimed to have used a rubber mallet to align a Siemens microscope, reminds me of my own experience along that line. I took mu first electron micrographs on an RCA EMB microscope in Robley Williams lab in the Physics Department here at the University of Michigan. This was only the second EM manufactured and sold by RCA. Robley had two of these instruments, one which he reserved for his own use and one that he allowed other 'qualified' persons to use. It was said that he selected the best pole pieces and power supplies from the two instruments and incorporated them to his own, and so he had one of the best electron microscopes in the country at the time. I was deemed a qualified user because I was studying under Professor L. O. Brockway who was internationally famous for his work on determining the structures of organic molecules by electron diffraction. Robley was, of course, probably best known for developing the method of shadow casting that was extensively used to enhance contrast in micrographs of particulate materials and surface replicas. At that time the tobacco mosaic virus, which have a beautiful long rod-like structure, was a favorite specimen to use to demonstrate the resolution of an EM. I remember a lecture on the merits of shadow casting where Robley showed a micrograph of beautifully shadowed long rod-like particles that everyone naturally assumed were tobacco mosaic virus; then Robley showed a second picture at higher magnification in which the particles could be clearly seen to be Lucky Strike cigarettes.
Anyway, halfway through the days of my graduate study Robley left the U of M, and his microscope was no longer available. To continue our work Professor Brockway managed to arrange for me to us an RCA EM2 electron microscope in the laboratory of Professor Thomas Francis in the School of Public Health (Prof Francis was famous for heading up the studies that confirmed the effectiveness of the Salk polio vaccine.) This instrument was a considerable improvement over the EMB; however, here is where the hammer-for-alignment feature came in. The condenser aperture on this model instrument did not have external controls for centering the condenser aperture, The aperture was merely a circular platinum disk with a small hole in the middle that was mounted in a cavity in the top of the condenser pole piece, and held in place with a retaining screw. Initially, the aperture had to be centered by trial and error, which involved disassembling the top of the column, removing the pole piece from the condenser lens, moving the aperture a bit, and then reassembling the instrument. To get a really good alignment of the aperture usually required several cycles of this routine combined with considerable luck. It was thus very tempting to work with dirty condenser apertures. FINALLY, however, someone (and I don't know that I ever heard who the sainted individual was) discovered that if the retaining screw was not tightened securely, so that the aperture could move around a bit, the aperture could be centered by tapping gently on the outside of the column. I don't recall ever using a rubber mallet, but the end of the handle of a screw driver did the job very well. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 15 -- From bigelow-at-umich.edu Thu Jan 20 13:42:35 2011 2, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 2, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KJgZBh018089 2, 15 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 13:42:35 -0600 2, 15 -- Received: FROM [76.234.170.127] (adsl-76-234-170-127.dsl.sfldmi.sbcglobal.net [76.234.170.127]) 2, 15 -- By hackers.mr.itd.umich.edu ID 4D38902A.A6C7B.20599 ; 2, 15 -- Authuser bigelow; 2, 15 -- 20 Jan 2011 14:42:34 EST 2, 15 -- Mime-Version: 1.0 2, 15 -- Message-Id: {p06240800c95e34c677dc-at-[76.226.70.137]} 2, 15 -- Date: Thu, 20 Jan 2011 14:42:08 -0500 2, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 15 -- Subject: [Microscopy]RE:Early Micro 2, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Dr. Mardinly's comments about using a hammer to dislodge whiskers inside an ion pump leads me to point out that if the whiskers are not too firmly established it is often possible to get rid of them by turning the high voltage supply on for a few seconds three or four times with the pressure in the pump above 1 Pa (10-2 Torr), If this approach works it is gentler, and quieter, than the hammer method. Incidentally, this method (but not the hammer method) and other characteristics of ion pumps, are described on page 295 of my book, 'Vacuum Methods in Electron Microscopy'.
Good luck! -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 2, 15 -- From bigelow-at-umich.edu Thu Jan 20 14:05:08 2011 2, 15 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.12.86]) 2, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KK58DA002555 2, 15 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 14:05:08 -0600 2, 15 -- Received: FROM [76.234.170.127] (adsl-76-234-170-127.dsl.sfldmi.sbcglobal.net [76.234.170.127]) 2, 15 -- By tombraider.mr.itd.umich.edu ID 4D389573.7D8CA.363 ; 2, 15 -- Authuser bigelow; 2, 15 -- 20 Jan 2011 15:05:07 EST 2, 15 -- Mime-Version: 1.0 2, 15 -- Message-Id: {p06240802c95e4334d9d3-at-[76.234.170.127]} 2, 15 -- Date: Thu, 20 Jan 2011 15:04:41 -0500 2, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 2, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 2, 15 -- Subject: Hammering ion pumps 2, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Frank, it is good to see someone putting some thought into the process. I have had numerous students/clients come to me and ask for an analysis with some particular parameters only for me to ask if they really wanted to invest that much time and money. (Maybe I shouldn't have asked. I could have just given them the bill telling them that I only did what they asked. Naahh.)
Your interaction volume estimate seems reasonable, but you may need to look into cutting your voltage to cut down the volume. I would think 15 kV or even 12 kV would still get you a good response for most K-lines and would really cut down the volume.
Like Bill said, you want to match the volume to the feature size. That is the bottom line.
You probably also want to match the pixel size to the volume, but there is something to be said for oversampling. Even though the volume will be broader than your pixel, a lot of the interaction helps as the core. Yes, you will get stray signals from the surrounding area. You won't be able to say that the volume does not contain this element or that, but you will stand a better chance at finding what is elevated at that location as opposed to stepping over a feature and missing it.
Now as to your specific numbers, let me examine them.
You say that your pixel size is 0.8 um and there are 1024 across the image, so your field of view is roughly 800 um. I generally calculate magnification against a standard width of 120 mm (the width of a Polaroid print). That gives me a magnification of 150x. Now, I suppose if your image was displayed 560 mm wide on a 22-inch wide screen, it would be accurate to state it was at 700x magnification. Otherwise, there may be something wrong in your setup. It would be worth checking. However, your calculations of 2.3 um pixels with 512 of them across a 100x image does make mathematical sense.
(BTW, I am an old fogey in wanting to reference everything to a 5-inch Polaroid print. It doesn't matter if it's a thumbnail of an image on the web or in a journal or if it's plastered across a billboard. I tend to count all pictures as 5 inches wide in my mind's eye. Therefore, I have my SEM setup to calculate that way.)
I would probably setup for about 0.5 to 1.0 um steps across the boundaries. I would cut that even finer at lower voltages. (Indeed, I have such a sample in my queue.) I don't normally setup my pixels as my step sizes. They don't have to match unless you are collecting a spectral image or an x-ray map. Then I would want to match my pixel to my interaction volume or a bit smaller to make sure I have sampled everything well when in hunting mode.
Spectral images or x-ray maps do take considerably more time to collect. In that sense, I am with Bill to recommend that you chose your points wisely. You could probably collect good data much faster. I am not against elemental maps. Sometimes they are the best way to make a point, but they usually require pretty marked changes in concentration to perceive a change in intensity. For general work, I restrict myself to manually placed points or to a small raster of points in a specific region of interest.
Not knowing how your precipitates (might) occur, I can't give you a particular recommendation. I would be imaging as well as I could, probably in BSE mode, looking for visible signs of the particles and then using EDS to identify and quantify the material.
Dear Frank, The key is that you're "looking for slight changes in element concentrations at grain boundaries and precipitates." In order best to see these changes, the pixel size should be smaller than the features--half the feature size guarantees that at least one pixel will be entirely within the feature. If the pixel size is too large than you will measure the average element concentrations over both the feature and the surrounding area, which will make the apparent changes smaller than they really are. I would suggest taking an image to locate the features, then place the beam on each feature in turn to do the analysis. Also take a spectrum of the area next to each feature. This should give you the best chance of seeing differences and minimize the amount of data. Yours, Bill from: Frank_Karl-at-lincolnelectric.com
I've been thinking about EDS line scans and I'm attempting to maximize The data I collect.
I run at 20 KV on iron samples so my electron interaction range is between 1.5 to 2.3um (Berthe or K-O range calculations). According to data provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at 700x. So right away I see if each image pixel represents a data point, I'm sampling about 2 pixel volumes per data point. Looking for a sudden and sharp change in element concentration seem difficult, but wait there's more. Let us introduce another plot complication.
My customers don't want to scan the entire image, tooo much data!
So I scan a smaller section of image described above and instruct the computer to take 512 data points along a line that might only be 400 (visual estimate from screen) image pixels long.
So... Should I reduce the image resolution so that each image pixel will be one data point and scan the entire image? Would it be better to lower the magnification so the image pixels are larger (say 1.2um ). I could go to 100x and 512 horizontal image pixel so each image pixel is 2.3um?
I'm looking for slight changes in element concentration at grain boundaries and precipitates and of course I want to produce the most meaningful data possible. I'm running at conditions that suggest my electron interactive volume is larger than my spatial resolution as to produce "continuous" data.
Or am I just over thinking the process?
Any suggestion or comments would be welcome
Frank Lincoln Electric Company -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 11, 17 -- From frank_karl-at-lincolnelectric.com Wed Jan 19 07:37:23 2011 11, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 11, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JDbNoO031503 11, 17 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 07:37:23 -0600 11, 17 -- Subject: EDS line scans and time on my hands 11, 17 -- To: Microscopy-at-microscopy.com 11, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 11, 17 -- Message-ID: {OF4A811DE5.D47D1408-ON8525781D.004A569D-8525781D.004AD372-at-lincolnelectric.com} 11, 17 -- From: Frank_Karl-at-lincolnelectric.com 11, 17 -- Date: Wed, 19 Jan 2011 08:38:03 -0500 11, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 11, 17 -- 01/19/2011 08:37:18 AM 11, 17 -- MIME-Version: 1.0 11, 17 -- Content-Type: text/plain; 11, 17 -- charset="UTF-8" 11, 17 -- Content-Transfer-Encoding: 8bit 11, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0JDbNoO031503 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L4u7n7019489 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 20 Jan 2011 22:56:07 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p0L4u7Rk019487; Thu, 20 Jan 2011 22:56:07 -0600
Hello Dr. Zhou,
Our research group has also struggled with the challenge of obtaining quality diffraction patterns from fine precipitates. The problem is magnified (no pun intended) when the precipitates are very small, less than 5nm in diameter, or when there is a high concentration of precipitates with potentially different chemistry and crystal structures (as is often the case with carbon replicas). The latter problem prohibits the use of selected area diffraction techniques for identifying individual precipitates as you will collect information from many different precipitates with even the smallest SA aperture. I'm only slightly surprised that you haven't been able to achieve good results using nano beam diffraction. We've found that vanadium and chromium-rich carbides are rapidly degraded by the electron beam. The nano beam or convergent beam probe tends to damage the crystal structure of the carbides as soon as the probe touches the precipitate. We're not sure whether radiolysis or knock-on damage is the main culprit since we've obtained less than ideal results at both 80kV and at 200kV. We have resorted to imaging the precipitates with HRTEM and interpreting the FFTs to determine structure but we're still making some attempts to use convergent beam probes to more rapidly acquire diffraction patterns.
Good luck and keep us updated of your successes, Chris Drexel University - Dynamic Characterization Group
On Wed, Jan 19, 2011 at 3:47 PM, {z.zhou-at-lboro.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://www.microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: z.zhou-at-lboro.ac.uk } Name: Zhou } } Organization: Dept of Materials, Loughborough University, UK } } Title-Subject: [Filtered] TEM diffraction 3nm precipitates } } Message: Greetings listers, } } Iím studying crystal structure of some } carbides/nitrides precipitates in steel. The } precipitates are 2-5nm size in a C replica TEM } specimen, V-, Cr-, and Nb-rich by STEM/EDX. } } How can I get electron diffraction or crystal } structure information of these fine precipitates? } } I tried on a TecnaiF20 TEM using nanoprobe spot } 7, focused on a small particle, then diffraction, } most of the time I got amorphous rings of C } support, no obvious diffraction discs. STEM mode } did indicate some C contamination on the session. } Assuming minimal C contamination, is it possible } that this method can give me some sort of } convergence beam electron diffraction pattern, } which may help me determine the crystal structure } of the precipitates? } } Iím also open to other methods. } } Your advice is highly appreciated. } } Zhou } } Dr Zhaoxia Zhou } Dept of Materials } Loughborough University } Loughborough, UK } LE11 3TU } } } Login Host: 158.125.68.189 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 15, 24 -- From zaluzec-at-microscopy.com Wed Jan 19 14:33:42 2011 } 15, 24 -- Received: from znl.com ([206.69.208.20]) } 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JKXf70014726 } 15, 24 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:42 -0600 } 15, 24 -- Received: from localhost (localhost [127.0.0.1]) } 15, 24 -- by znl.com (Postfix) with ESMTP id E7C66316930 } 15, 24 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:41 -0600 (CST) } 15, 24 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 15, 24 -- Received: from znl.com ([127.0.0.1]) } 15, 24 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 15, 24 -- with ESMTP id yEihmXkByYx9 for {microscopy-at-microscopy.com} ; } 15, 24 -- Wed, 19 Jan 2011 14:33:41 -0600 (CST) } 15, 24 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 15, 24 -- by znl.com (Postfix) with ESMTPA id 6BC61316925 } 15, 24 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 14:33:41 -0600 (CST) } 15, 24 -- Mime-Version: 1.0 } 15, 24 -- Message-Id: {p06240802c95cfb015564-at-[206.69.208.22]} } 15, 24 -- Date: Wed, 19 Jan 2011 14:33:39 -0600 } 15, 24 -- To: microscopy-at-microscopy.com } 15, 24 -- From: z.zhou-at-lboro.ac.uk (by way of MicroscopyListserver) } 15, 24 -- Subject: viaWWW: TEM diffraction 3nm precipitates } 15, 24 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" } 15, 24 -- Content-Transfer-Encoding: 8bit } 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JKXf70014726 } ==============================End of - Headers============================== }
-- Christopher Winkler Dynamic Characterization Group http://www.materials.drexel.edu/dcg/ Drexel University 267-496-0587
==============================Original Headers============================== 8, 35 -- From microwink-at-gmail.com Thu Jan 20 22:56:07 2011 8, 35 -- Received: from mail-ey0-f169.google.com (mail-ey0-f169.google.com [209.85.215.169]) 8, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L4u6QQ019484 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 22:56:07 -0600 8, 35 -- Received: by eyh6 with SMTP id 6so713378eyh.0 8, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 20:56:06 -0800 (PST) 8, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 35 -- d=gmail.com; s=gamma; 8, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 8, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 8, 35 -- bh=Ks4kqeKtcXfsD3A3QeUaeDTbU0bJl/soHZZfdAp+8Nc=; 8, 35 -- b=DU+IKIzBMJIW4M1C2TAWQg3lfaT0uLAFnFgiEtj0+P2usuVbQ145R46PLSQCEJhx94 8, 35 -- 2OvkTVwh052NiA0opZkD/rtpJVT46jCxIgfR07HDOJsRkk8hQ3RthWZzZeGkXv+E9gFD 8, 35 -- nS3+YMfVxpyj6xaHxnnL58Ebpx1y2nkwBEfaM= 8, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 8, 35 -- d=gmail.com; s=gamma; 8, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 8, 35 -- :content-type:content-transfer-encoding; 8, 35 -- b=QL/B7uvHE0Bn2DMCSoQM83OC8PwvjdHbgPfGR1gKwRt78xW3pgzfUBhwOL7R7tMP5K 8, 35 -- 6ANIdnuFOL5LiP92RDhLdNRR1nBNI6/SzhEzGLeutxuENnkBtGKcNJghnj1lf8KVtUqg 8, 35 -- MlmmbCdVf+wzcSV9BdpV6MZI9y6fn52NZKvFQ= 8, 35 -- MIME-Version: 1.0 8, 35 -- Received: by 10.14.17.225 with SMTP id j73mr192084eej.26.1295585765587; Thu, 8, 35 -- 20 Jan 2011 20:56:05 -0800 (PST) 8, 35 -- Received: by 10.14.119.141 with HTTP; Thu, 20 Jan 2011 20:56:05 -0800 (PST) 8, 35 -- In-Reply-To: {201101192047.p0JKl39u003981-at-ns.microscopy.com} 8, 35 -- References: {201101192047.p0JKl39u003981-at-ns.microscopy.com} 8, 35 -- Date: Thu, 20 Jan 2011 23:56:05 -0500 8, 35 -- Message-ID: {AANLkTimhg7J6h8WBZ7kiTZLC4U1FeLLH3PjExSDupYcf-at-mail.gmail.com} 8, 35 -- Subject: Re: [Microscopy] viaWWW: TEM diffraction 3nm precipitates 8, 35 -- From: Christopher Winkler {microwink-at-gmail.com} 8, 35 -- To: Microscopy-at-microscopy.com 8, 35 -- Content-Type: text/plain; charset=ISO-8859-1 8, 35 -- Content-Transfer-Encoding: 8bit 8, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L4u6QQ019484 ==============================End of - Headers==============================
I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 6, 37 -- From kraftpiano-at-gmail.com Thu Jan 20 23:08:26 2011 6, 37 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L58Q25003006 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 23:08:26 -0600 6, 37 -- Received: by yxl31 with SMTP id 31so469145yxl.0 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 21:08:26 -0800 (PST) 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 6, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 6, 37 -- bh=0UOwJelpZ6QJtvR6h504fJNBtAxqg11VNSbyg5wJQ74=; 6, 37 -- b=CKvCVRNoVTsFwRnLpU5lCXf8YvPMxH1N3dS7ys8UPf3yiqhPDWGbMo0obmG88cgOgo 6, 37 -- XZULU3FfCYHsarDBM9jexEC0W3v5YP7EFDMCVYhQlXG/1kA7Yhd73anJHaX3ZIz+H7uz 6, 37 -- JgDVe+ofiVTeCAAmXGLSf7iUKv6XvcnYIVDww= 6, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 6, 37 -- :to:mime-version:x-mailer; 6, 37 -- b=PR17AmFsaRKhUKvApaUTF9Rk1PZybTM2uGPbnyI+JSfekwjDyixYQTfQWeBGOPBeUR 6, 37 -- jtKbSKxwwTpYoyFNLlhFZrNVcU8rUujb+K2L3feJgIONXADzcGx34Xj28IMbBnWZHLwQ 6, 37 -- 1EKfQrQ/cpSgDSCYg9bEB0VVr0WIDudjYmUZI= 6, 37 -- Received: by 10.151.68.12 with SMTP id v12mr198668ybk.387.1295586505917; 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 6, 37 -- by mx.google.com with ESMTPS id u10sm82704yba.13.2011.01.20.21.08.24 6, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 6, 37 -- Content-Type: text/plain; charset=us-ascii 6, 37 -- Subject: STM tip advice. 6, 37 -- Date: Fri, 21 Jan 2011 00:08:23 -0500 6, 37 -- Message-Id: {EA4CFA28-451C-4E4D-A543-EEA026824B89-at-gmail.com} 6, 37 -- To: microscopy-at-microscopy.com 6, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 37 -- X-Mailer: Apple Mail (2.1082) 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L58Q25003006 ==============================End of - Headers==============================
Justin: I used to make my own probe tips (for poking around inside an IC) with tungsten wire and KOH. We had a set-up under a microscope that applied a voltage (I think it was 5 volts and an amp or so but don't remember exactly) between the wire and the KOH (in a metal container) and you moved the wire in and out of the KOH to form the tip. The surface tension of the KOH as the wire moves in and out forms a nice pointy tip. It's best to have something that controls the position and movement of the wire mechanically unless you have very steady hands. Welding the wire to the KOH container at 10-20X magnification will you make you jump!
On 1/20/11 11:08 PM, kraftpiano-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf). } } I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution. } } The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses. } } Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway. } } Thanks, } } Justin A. Kraft } } ==============================Original Headers============================== } 6, 37 -- From kraftpiano-at-gmail.com Thu Jan 20 23:08:26 2011 } 6, 37 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) } 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L58Q25003006 } 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 23:08:26 -0600 } 6, 37 -- Received: by yxl31 with SMTP id 31so469145yxl.0 } 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 21:08:26 -0800 (PST) } 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 37 -- d=gmail.com; s=gamma; } 6, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding } 6, 37 -- :subject:date:message-id:to:mime-version:x-mailer; } 6, 37 -- bh=0UOwJelpZ6QJtvR6h504fJNBtAxqg11VNSbyg5wJQ74=; } 6, 37 -- b=CKvCVRNoVTsFwRnLpU5lCXf8YvPMxH1N3dS7ys8UPf3yiqhPDWGbMo0obmG88cgOgo } 6, 37 -- XZULU3FfCYHsarDBM9jexEC0W3v5YP7EFDMCVYhQlXG/1kA7Yhd73anJHaX3ZIz+H7uz } 6, 37 -- JgDVe+ofiVTeCAAmXGLSf7iUKv6XvcnYIVDww= } 6, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 6, 37 -- d=gmail.com; s=gamma; } 6, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id } 6, 37 -- :to:mime-version:x-mailer; } 6, 37 -- b=PR17AmFsaRKhUKvApaUTF9Rk1PZybTM2uGPbnyI+JSfekwjDyixYQTfQWeBGOPBeUR } 6, 37 -- jtKbSKxwwTpYoyFNLlhFZrNVcU8rUujb+K2L3feJgIONXADzcGx34Xj28IMbBnWZHLwQ } 6, 37 -- 1EKfQrQ/cpSgDSCYg9bEB0VVr0WIDudjYmUZI= } 6, 37 -- Received: by 10.151.68.12 with SMTP id v12mr198668ybk.387.1295586505917; } 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) } 6, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) } 6, 37 -- by mx.google.com with ESMTPS id u10sm82704yba.13.2011.01.20.21.08.24 } 6, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); } 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) } 6, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} } 6, 37 -- Content-Type: text/plain; charset=us-ascii } 6, 37 -- Subject: STM tip advice. } 6, 37 -- Date: Fri, 21 Jan 2011 00:08:23 -0500 } 6, 37 -- Message-Id: {EA4CFA28-451C-4E4D-A543-EEA026824B89-at-gmail.com} } 6, 37 -- To: microscopy-at-microscopy.com } 6, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) } 6, 37 -- X-Mailer: Apple Mail (2.1082) } 6, 37 -- Content-Transfer-Encoding: 8bit } 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L58Q25003006 } ==============================End of - Headers============================== }
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 4, 23 -- From r-holdford-at-ti.com Fri Jan 21 02:06:37 2011 4, 23 -- Received: from bear.ext.ti.com (bear.ext.ti.com [192.94.94.41]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L86bjR023049 4, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 02:06:37 -0600 4, 23 -- Received: from dlep33.itg.ti.com ([157.170.170.112]) 4, 23 -- by bear.ext.ti.com (8.13.7/8.13.7) with ESMTP id p0L86ZUd007341 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 23 -- Fri, 21 Jan 2011 02:06:35 -0600 4, 23 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 4, 23 -- by dlep33.itg.ti.com (8.13.7/8.13.7) with ESMTP id p0L86Zrf004655; 4, 23 -- Fri, 21 Jan 2011 02:06:35 -0600 (CST) 4, 23 -- Message-ID: {4D393E8B.4010507-at-ti.com} 4, 23 -- Date: Fri, 21 Jan 2011 02:06:35 -0600 4, 23 -- From: Becky Holdford {r-holdford-at-ti.com} 4, 23 -- Organization: SC Packaging MPI Team 4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 4, 23 -- MIME-Version: 1.0 4, 23 -- To: kraftpiano-at-gmail.com, MSA Listserver {Microscopy-at-microscopy.com} 4, 23 -- Subject: Re: [Microscopy] STM tip advice. 4, 23 -- References: {201101210508.p0L58XuH003221-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {201101210508.p0L58XuH003221-at-ns.microscopy.com} 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello, One of my friends told me that in the sixties, he and his colleagues were looking for decommissioned broken black glass covers of gravestones. According to him, it was the best material for glass knives.
Oldrich
On Thursday 20 of January 2011 16:54:51 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } I know at least 2 old microscopists who made } glass knifes by breaking window glass - one of } them here at CMU. He went to construction sites } to get the broken windows. } } I'm running down the reference and (I hope) an } image, but one of the early tries at } ultramicrotomy was sticking razor blades on a } centrifuge rotor, then mount the sections on the } tub, close the lid and turn on the centrifuge. } The sections where then picked up from inside the } tub, having been flung willy-nilly around the } inside. } (The image is used in the microtomy lecture to } convince the students thin sectioning could be a } lot worse than they think it is.) } } Phil } } } Von: Muþ Wolfgang } } Gesendet: Donnerstag, 20. J”nner 2011 11:46 } } An: 'joelsheffield-at-gmail.com' } } Cc: 'microscopy-at-microscopy.com' } } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska" } } } } Joel wrote: } } } Now, the urban legend.Ý } } } } I had heard that a group (unnamed) was having a } } vigorous discussion about the best way to strop } } a steel knife so as to get the best edge for } } cutting thin sections.Ý How many strokes, in } } which direction, which side of the blade, etc.Ý } } During the discussion, a bottle of Coke was } } knocked off the table and broke into many } } pieces.Ý } } The story goes that one, or another, of these } } people took up one of the shards, and suggested } } that it would make a good knife edge. } } Verification anyone? { } } } } Joel } } } } } } Good morning, } } Dear Joel, dear all } } } } I can't tell or verify the above mentioned } } "Coke-shard-story" but - indeed - I can add } } information as to the "knocked off } } table"-part.... } } } } I had always heared (personal informations from } } elder REICHERT-freaks and service people, from } } lectures/lecturers in the 70ies and 80ies as } } well as personally from H. Sitte himself) that } } the group around H. SITTE at REICHERT (now } } LEICA) in the late 1950ies/60ies was/were doing } } such experiments with {glass plates} .... which } } were thrown down to the floor... and one of them } } (it was Sitte himself, as he told me) picked up } } the shards evaluating the edges of "the most } } promising one" for sectioning purposes (who of } } that group had the "invention" to use glass as } } a} crude { knife (element) I unfortunately don't } } know. } } } } By the way, digging a little bit on this in } } Google, I found one hint on a document in a } } German data bank at } } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.Ý } } } } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver–ffentlicht } } ("A simple ultramicrotome with thermal advance" published) } } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367 } } } } Note:Ý {Naturwissenschaften} is the former title } } ofÝÝÝÝÝ Biomedical and Life SciencesÝ (SPRINGER) } } Another source:Ý http://www.freepatentsonline.com/3828641.html: } } United States Patent US3828641: } } } } ==} Apparatus for adjusting the elevation of a } } specimen in microtomes, particularly } } ultramicrotomes } } Inventor:Ý Hellmuth Sitte, HOMBURG (not Humburg } } [as written in the patent document!]/Saar } } Germany) } } Assignee:Ý C.Reichert Optische Werke AG, Vienna, Austria) } } Filed:Ý Nov. 10,1972 } } } } Also see } } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+a } } nd+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8 } } &hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ve } } d=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false (From the } } book Michael J. Dykstra, Laura E. } } Reuss - 2003Ý (see page "History: in the text } } displayed } } the Reichert OMU-1 (before 1964, perhaps see } } document above in {Naturwissenschaften} 1955) } } from Sitte described as having thermal advance, } } Sitte was never happy with, since 1964: OMU-2 } } with thermal advance.... } } } } This just for your pleasure.... } } (and I sure that searching for Sitte Hellmuth or } } Sitte and microtome will find some results more } } on that interesting and exciting "history" of } } ultramicrotomy advances..... } } } } Best wishes and regards, } } have a good and successful rest of the week and a beautiful weekend, } } } } Wolfgang MUSS (Ph.D.) } } EM-Lab Gen.Hosp. SALK-LKH } } SALZBURG } } AUSTRIA } } } } } -----Urspr¸ngliche Nachricht----- } } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com] } } } Gesendet: Donnerstag, 20. J”nner 2011 06:51 } } } An: Muþ Wolfgang } } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska" } } } } } } ----------------------------------------------------------------------- } } } ----- } } } The Microscopy ListServer -- CoSponsor:Ý The Microscopy Society of } } } America } } } ToÝ Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } } } ----- } } } } } } It is certainly clear that there are many interesting stories --some } } } urban legends, and some insights into the minds of those that founded } } } the discipline of thin section electron microscopy. } } } During the mid '70's, I taught an EM course at Rutgers, Camden. } } } In the basement, along with an RCA EMU-2 (!) was a thermal advance } } } microtome that I was told was developed by Sjostrand. } } } This thing consisted of a disk-like plate, about 4-5 inches in } } } diameter, with an eccentrically mounted arm that stuck out of the } } } plate.Ý The arm had a chuck for a tissue sample, and a heating coil. } } } The plate itself was mounted in a frame and lubricated with oil --the } } } plate acted like a large oil bearing.Ý The plate was linked to a motor } } } on the wall by a long V belt (to minimize vibration), and the entire } } } plate rotated, bringing the block past the knife on each rotation.Ý The } } } sections that resulted tended to be arc shaped.Ý We got it working, but } } } it was a terrifying thing to see.Ý I wonder if anyone in this group } } } remembers this type of machine -- assuming that my description is } } } comprehensible. } } } } } } The other microtome that I used, and really admire, was the Huxley } } } microtome, originally sold by Cambridge, and ultimately marketed by } } } LKB.Ý Although it used a mechanical advance, all of the movements of } } } the cutting arm were made by bending sheets of spring steel, so that } } } there were no surfaces that moved against each other.Ý Moreover, the } } } cutting stroke was controlled by an oil-filled damper, to minimize } } } chatter. -- a remarkable and stable machine. } } } } } } Now, the urban legend. } } } I had heard that a group (unnamed) was having a vigorous discussion } } } about the best way to strop a steel knife so as to get the best edge } } } for cutting thin sections.Ý How many strokes, in which direction, which } } } side of the blade, etc.Ý During the discussion, a bottle of Coke was } } } knocked off the table and broke into many pieces. } } } The story goes that one, or another, of these people took up one of the } } } shards, and suggested that it would make a good knife edge. } } } Verification anyone? } } } } } } Joel } } } } } } -- } } } Joel B. Sheffield, Ph.D. } } } Biology Department, Temple University } } } 1900 North 12th Street } } } Philadelphia, PA 19122 } } } jbs-at-temple.edu } } } (215) 204 8839, fax (215) 204 0486 } } } http://astro.temple.edu/~jbs
==============================Original Headers============================== 4, 23 -- From benada-at-biomed.cas.cz Fri Jan 21 03:07:43 2011 4, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L97gOW007522 4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 03:07:43 -0600 4, 23 -- Received: from u117ob.localnet (a100ix.mbu.cas.cz [147.231.44.171]) 4, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 23 -- (No client certificate requested) 4, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id D06561A446DE 4, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 10:07:41 +0100 (CET) 4, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 23 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 4, 23 -- To: microscopy-at-microscopy.com 4, 23 -- Subject: Re: [Microscopy] Re: Microtome History - was "Ernst Ruska" 4, 23 -- Date: Fri, 21 Jan 2011 10:07:31 +0100 4, 23 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 4, 23 -- References: {201101201554.p0KFspb2001352-at-ns.microscopy.com} 4, 23 -- In-Reply-To: {201101201554.p0KFspb2001352-at-ns.microscopy.com} 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Type: Text/Plain; 4, 23 -- charset="windows-1250" 4, 23 -- Message-Id: {201101211007.31576.benada-at-biomed.cas.cz} 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L97gOW007522 ==============================End of - Headers==============================
All I have been watching the advice on this problem and whilst I agree with all that has been said may I offer a little more?
I have had experience with materials that forced us to use cryo within an investigation, then we (the client and I) purchased an early FEGSEM. As you may guess the cryo kit would not fit onto the FEG! Forced to take another route we found that being very careful in the FEG we could work on the very sensitive material without any apparent problems.
The settings we used were those typical for sensitive materials:-
1. Coat the specimen with a minimal current and an extended working distance e.g. normal would be ~5cm we used 7cm from memory(?) 2. Use a low emission current. 3. Use a spot size/probe current near the lowest limit of the instrument. 4. Use the out of lens detector for minimal charge (~15mm WD)
I hope that this may help?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: 20 January 2011 17:58 To: protrain-at-emcourses.com
Dear All, I had the same problem visualizing bee wax structures and not owning an ESEM ;-)
Solution: I put the structure on a large specimen mount, putting 2 bands of conductive adhesive tape left and right on top and put it in my normal Pt-sputtercoater. Concerning the heat during sputtering, I took off the sputter-head and added a second glass vessel (I took it from my carbon-coater) on top of the first. Did ca. 10-15 sputtering-runs with minimal current (10mA) and finally put it in the scope at 5-6kV.
See images at http://www.electronmicroscopy.info/wax
It`s a bit of a crude solution but it works. Concerning the thickness of your wax layers you may also try the same thing with an high-vac chromecoater...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I'm not a biologist (most materials science) nor experimented in ESEM (only high vac SEM), but I've on my shelve a thermoelectric module (Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.
Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf, where you can find a great variety of such devices. They give a Dt° in the 70°C range, and are avaible in several dimensions and power. From 10x10 to 62x62 are proposed, but rectanglaire or round shapes too. Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the hot side is low. There is probably room enough to put a heat sink on the stage (copper block), but possibly one would need a water cooled one, what is probably not necessary on the device provided by FEI. If you have the possibilty to build something yourselve, it could be much cheaper. Of coarse without performences waranty !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter. } } You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe. } } Does anyone know if this means the sample can be any size one likes? } } Dave } } -----Original Message----- } X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] } Sent: 19 January 2011 22:53 } To: David Patton } Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu } Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Environmental SEM for Biological Work } } Message: Is there heavy use of ESEM for biological SEM? What is } commonly done on biological ESEM samples? We're looking at } purchasing an environmental SEM and the sales rep. tells us we will } need a Peltier stage to cool the sample to look at wet samples. } Unfortunately, the maximum sample size for the Peltier stage is 3 mm } across. This seems tiny to me for an SEM. Are there many biological } ESEM applications that don't require the sample to be wet? } } Thanks, } } Robin Foley (who spends most of her SEM time looking at metals, } ceramics and polymers!) } } } } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 22 -- From zaluzec-at-microscopy.com Wed Jan 19 16:50:11 2011 } 11, 22 -- Received: from znl.com ([206.69.208.20]) } 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JMoBNn026165 } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 } 11, 22 -- Received: from localhost (localhost [127.0.0.1]) } 11, 22 -- by znl.com (Postfix) with ESMTP id 89D5B316AFA } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 22 -- Received: from znl.com ([127.0.0.1]) } 11, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 22 -- with ESMTP id UF6QAiTpp+Ew for {microscopy-at-microscopy.com} ; } 11, 22 -- Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 22 -- by znl.com (Postfix) with ESMTPA id DBEA8316AEF } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:09 -0600 (CST) } 11, 22 -- Mime-Version: 1.0 } 11, 22 -- Message-Id: {p0624080ec95d1b09d749-at-[206.69.208.22]} } 11, 22 -- Date: Wed, 19 Jan 2011 16:50:07 -0600 } 11, 22 -- To: microscopy-at-microscopy.com } 11, 22 -- From: rfoley-at-uab.edu (by way of MicroscopyListserver) } 11, 22 -- Subject: viaWWW: Environmental SEM for Biological Work } 11, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 48 -- From David.Patton-at-uwe.ac.uk Thu Jan 20 05:52:55 2011 } 23, 48 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1outboundpool.messaging.microsoft.com [157.55.116.155]) } 23, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KBqtKA009538 } 23, 48 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 05:52:55 -0600 } 23, 48 -- Received: from mail131-ch1-R.bigfish.com (216.32.181.172) by } 23, 48 -- CH1EHSOBE007.bigfish.com (10.43.70.57) with Microsoft SMTP Server id } 23, 48 -- 14.1.225.8; Thu, 20 Jan 2011 11:52:54 +0000 } 23, 48 -- Received: from mail131-ch1 (localhost.localdomain [127.0.0.1]) by } 23, 48 -- mail131-ch1-R.bigfish.com (Postfix) with ESMTP id 749AC86020F; Thu, 20 Jan } 23, 48 -- 2011 11:52:54 +0000 (UTC) } 23, 48 -- X-SpamScore: -23 } 23, 48 -- X-BigFish: VPS-23(zz7f0R542N4015L62a3Lzz1202hz31iz8275bhz2fh668h61h) } 23, 48 -- X-Spam-TCS-SCL: 0:0 } 23, 48 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:mailapp03.uwe.ac.uk;RD:mailapp03.uwe.ac.uk;EFVD:NLI } 23, 48 -- Received: from mail131-ch1 (localhost.localdomain [127.0.0.1]) by mail131-ch1 } 23, 48 -- (MessageSwitch) id 129552437448033_30359; Thu, 20 Jan 2011 11:52:54 +0000 } 23, 48 -- (UTC) } 23, 48 -- Received: from CH1EHSMHS030.bigfish.com (snatpool1.int.messaging.microsoft.com } 23, 48 -- [10.43.68.245]) by mail131-ch1.bigfish.com (Postfix) with ESMTP id } 23, 48 -- 0A59516B804E; Thu, 20 Jan 2011 11:52:54 +0000 (UTC) } 23, 48 -- Received: from mailapp03.uwe.ac.uk (164.11.132.65) by CH1EHSMHS030.bigfish.com } 23, 48 -- (10.43.70.30) with Microsoft SMTP Server id 14.1.225.8; Thu, 20 Jan 2011 } 23, 48 -- 11:52:53 +0000 } 23, 48 -- Received: from (egen-hub02.uwe.ac.uk [164.11.251.46]) by mailapp03.uwe.ac.uk } 23, 48 -- with smtp id 7bd0_2fac_cfd14bd6_248b_11e0_8ec4_00142221cca9; Thu, 20 Jan } 23, 48 -- 2011 11:52:52 +0000 } 23, 48 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 48 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.251.46]) with mapi; Thu, 20 Jan 2011 } 23, 48 -- 11:51:21 +0000 } 23, 48 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 48 -- To: "'rfoley-at-uab.edu'" {rfoley-at-uab.edu} } 23, 48 -- Date: Thu, 20 Jan 2011 11:51:20 +0000 } 23, 48 -- Subject: RE: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Topic: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Index: Acu4K66MlRtfppFTQyqwkR6SfbYJwwAa6AKw } 23, 48 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF433F9B96D72-at-EGEN-MBX02.campus.ads.uwe.ac.uk} } 23, 48 -- References: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- In-Reply-To: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- Accept-Language: en-US, en-GB } 23, 48 -- Content-Language: en-US } 23, 48 -- X-MS-Has-Attach: } 23, 48 -- X-MS-TNEF-Correlator: } 23, 48 -- acceptlanguage: en-US, en-GB } 23, 48 -- Content-Type: text/plain; charset="us-ascii" } 23, 48 -- MIME-Version: 1.0 } 23, 48 -- X-OriginatorOrg: uwe.ac.uk } 23, 48 -- Content-Transfer-Encoding: 8bit } 23, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KBqtKA009538 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 21 09:29:38 2011 8, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LFTbmD020182 8, 30 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 09:29:37 -0600 8, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 30 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p0LFTZcQ065634 8, 30 -- ; Fri, 21 Jan 2011 16:29:35 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 8, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8CB321000139; 8, 30 -- Fri, 21 Jan 2011 16:28:58 +0100 (CET) 8, 30 -- Message-ID: {4D39A656.9060606-at-ipcms.u-strasbg.fr} 8, 30 -- Date: Fri, 21 Jan 2011 16:29:26 +0100 8, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 30 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.13) Gecko/20101208 Thunderbird/3.1.7 8, 30 -- MIME-Version: 1.0 8, 30 -- To: microscopy-at-microscopy.com, rfoley-at-uab.edu 8, 30 -- Subject: Re: Environmental SEM for Biological Work 8, 30 -- References: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-IPCMS-MailScanner: Found to be clean 8, 30 -- X-IPCMS-MailScanner-SpamScore: s 8, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 21 Jan 2011 16:29:35 +0100 (CET) 8, 30 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 8, 30 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 8, 30 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
Have you run spot analysis (boundary and middle of a grain) to confirm you can detect difference? From my experience with iron specimens, it is a pretty tough task to see boundary segregations even with WDS (at least when it is not a second phase, like carbides). X-from spot analysis you can estimate time needed for acquisition.
I do not know what element you are going to analyze, but if it is phosphorous, 20 kV is a way too high. For P you can use 4-5 kV and have a nice small zone of interaction.
Good luck
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} from: Frank_Karl-at-lincolnelectric.com } Date: 01/19/2011 05:45 AM } } I've been thinking about EDS line scans and I'm attempting to maximize } The data I collect. } } I run at 20 KV on iron samples so my electron interaction range is } between } 1.5 to 2.3um (Berthe or K-O range calculations). According to data } provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um } at } 700x. So right away I see if each image pixel represents a data point, } I'm } sampling about 2 pixel volumes per data point. Looking for a sudden } and } sharp change in element concentration seem difficult, but wait there's } more. Let us introduce another plot complication. } } My customers don't want to scan the entire image, tooo much data! } } So I scan a smaller section of image described above and instruct the } computer to take 512 data points along a line that might only be 400 } (visual estimate from screen) image pixels long. } } So... Should I reduce the image resolution so that each image pixel } will be } one data point and scan the entire image? Would it be better to lower } the } magnification so the image pixels are larger (say 1.2um ). I could go } to } 100x and 512 horizontal image pixel so each image pixel is 2.3um? } } I'm looking for slight changes in element concentration at grain } boundaries } and precipitates and of course I want to produce the most meaningful } data } possible. I'm running at conditions that suggest my electron } interactive } volume is larger than my spatial resolution as to produce "continuous" } data. } } Or am I just over thinking the process? } } Any suggestion or comments would be welcome } } Frank } Lincoln Electric Company } -- } ************************************************************* } Note: } The information contained in this message may be } privileged and confidential and protected from disclosure. If } the reader of this message is not the intended recipient, or } an employee or agent responsible for delivering this message } to the intended recipient, you are hereby notified that any } dissemination, distribution or copying of this communication } is strictly prohibited. If you have received this } communication in error, please notify us immediately by } replying to the message and deleting it from your computer. } Thank you, } The Lincoln Electric Company } **************************************************************
==============================Original Headers============================== 9, 32 -- From DusevichV-at-umkc.edu Fri Jan 21 10:44:52 2011 9, 32 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LGiqXx009517 9, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 10:44:52 -0600 9, 32 -- X-IronPort-Anti-Spam-Filtered: true 9, 32 -- X-IronPort-Anti-Spam-Result: Ag4FAOtGOU3RauUp/2dsb2JhbACeegeFZHO0dIhohVAEhG+JWw 9, 32 -- Received: from um-nsmtpout1.um.umsystem.edu ([209.106.229.41]) 9, 32 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 21 Jan 2011 10:44:51 -0600 9, 32 -- Received: from UM-NHUB02.um.umsystem.edu ([209.106.230.182]) by um-nsmtpout1.um.umsystem.edu with Microsoft SMTPSVC(6.0.3790.4675); 9, 32 -- Fri, 21 Jan 2011 10:44:51 -0600 9, 32 -- Received: from um-email06.um.umsystem.edu ([169.254.1.184]) by 9, 32 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Fri, 21 Jan 2011 9, 32 -- 10:44:51 -0600 9, 32 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 9, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 32 -- Date: Fri, 21 Jan 2011 10:44:49 -0600 9, 32 -- Subject: RE: [Microscopy] EDS line scans and time on my hands 9, 32 -- Thread-Topic: [Microscopy] EDS line scans and time on my hands 9, 32 -- Thread-Index: Acu5G40UOmZxAGn/TK6djOViess4DAAa+xlQ 9, 32 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3A99B58CF92-at-UM-EMAIL06.um.umsystem.edu} 9, 32 -- References: {201101210330.p0L3UR8p002174-at-ns.microscopy.com} 9, 32 -- In-Reply-To: {201101210330.p0L3UR8p002174-at-ns.microscopy.com} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- acceptlanguage: en-US 9, 32 -- Content-Type: text/plain; charset="us-ascii" 9, 32 -- MIME-Version: 1.0 9, 32 -- X-OriginalArrivalTime: 21 Jan 2011 16:44:51.0181 (UTC) FILETIME=[85CFE1D0:01CBB98A] 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LGiqXx009517 ==============================End of - Headers==============================
I replied to Debby Sherman offline. In general stool specimen suspensions can be fixed in 2.5-5% paraformaldehyde prior to shipping. The specimens are then rendered noninfectious and can be maintained for an indefinite period at 4 degrees C. Multiple freezing and thawing, on occasion, can cause deleterious effects to viruses sufficient to render them difficult to identify by diagnostic negative stain electron microscopy.
Best regards,
Charles
Charles Humphrey US Centers for Disease Control and Prevention Mailstop G32 1600 Clifton Rd. Atlanta, GA 30333
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, January 19, 2011 4:46 PM To: Humphrey, Charles (CDC/OID/NCEZID)
Hi all,
We will be imaging a fairly large number of norovirus samples using negative staining. This is just for sample screening to document the presence or absence of the virus.
The samples could be sent in fresh but I thought it might be better to fix them prior to shipping so that they are not a health hazard. That might also allow us to store them unfrozen for an extended period of time (months) with reduced chance of deterioration or contamination.
Does anyone have a validated protocol that they are willing to share? I used to know someone at the CDC who did TEM but no longer have that contact.
Thanks, Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 7, 28 -- From dsherman-at-purdue.edu Wed Jan 19 15:36:50 2011 7, 28 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JLanJM026947 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 15:36:50 -0600 7, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 7, 28 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p0JLan3m008331 7, 28 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:36:49 -0500 7, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.110]) by WPPEXHUB01F.purdue.lcl 7, 28 -- ([::1]) with mapi; Wed, 19 Jan 2011 16:36:48 -0500 7, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 7, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 7, 28 -- Date: Wed, 19 Jan 2011 16:36:46 -0500 7, 28 -- Subject: Protocol for Norovirus TEM 7, 28 -- Thread-Topic: Protocol for Norovirus TEM 7, 28 -- Thread-Index: Acu4IPihFFWUNBgITEScuEiQwrSisg== 7, 28 -- Message-ID: {C95CC39E.B10D%dsherman-at-purdue.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- X-PMX-Version: 5.5.9.388399 7, 28 -- X-PerlMx-Virus-Scanned: Yes 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0JLanJM026947 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 37 -- From cdh1-at-cdc.gov Fri Jan 21 11:51:58 2011 18, 37 -- Received: from mail165.messagelabs.com (mail165.messagelabs.com [216.82.253.147]) 18, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LHpw3e030635 18, 37 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 11:51:58 -0600 18, 37 -- X-VirusChecked: Checked 18, 37 -- X-Env-Sender: cdh1-at-cdc.gov 18, 37 -- X-Msg-Ref: server-9.tower-165.messagelabs.com!1295632310!40992952!7 18, 37 -- X-StarScan-Version: 6.2.9; banners=-,-,- 18, 37 -- X-Originating-IP: [158.111.190.156] 18, 37 -- Received: (qmail 16208 invoked from network); 21 Jan 2011 17:51:56 -0000 18, 37 -- Received: from unknown (HELO ECASHUB-CHAM3.cdc.gov) (158.111.190.156) 18, 37 -- by server-9.tower-165.messagelabs.com with AES128-SHA encrypted SMTP; 21 Jan 2011 17:51:56 -0000 18, 37 -- Received: from LTA3MF011.ees.hhs.gov (158.74.249.111) by ECASHUB-CHAM3.cdc.gov 18, 37 -- (158.111.190.156) with Microsoft SMTP Server id 14.0.702.0; Fri, 21 Jan 2011 18, 37 -- 12:51:04 -0500 18, 37 -- Received: from LTA3VS003.ees.hhs.gov ([158.74.248.102]) by 18, 37 -- LTA3MF011.ees.hhs.gov with Microsoft SMTPSVC(6.0.3790.3959); Fri, 21 Jan 18, 37 -- 2011 12:51:05 -0500 18, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 18, 37 -- Content-Class: urn:content-classes:message 18, 37 -- MIME-Version: 1.0 18, 37 -- Content-Type: text/plain; charset="us-ascii" 18, 37 -- Subject: RE: [Microscopy] Protocol for Norovirus TEM 18, 37 -- Date: Fri, 21 Jan 2011 12:51:04 -0500 18, 37 -- Message-ID: {CBC4D3A10E913147808B5E5070217BD70F464E-at-LTA3VS003.ees.hhs.gov} 18, 37 -- In-Reply-To: {201101192145.p0JLjbrm014166-at-ns.microscopy.com} 18, 37 -- X-MS-Has-Attach: 18, 37 -- X-MS-TNEF-Correlator: 18, 37 -- Thread-Topic: [Microscopy] Protocol for Norovirus TEM 18, 37 -- Thread-Index: Acu4IjzCHHjx0vmfTWmz+2MTRdZvVgBbx0SA 18, 37 -- References: {201101192145.p0JLjbrm014166-at-ns.microscopy.com} 18, 37 -- From: "Humphrey, Charles (CDC/OID/NCEZID)" {cdh1-at-cdc.gov} 18, 37 -- To: {dsherman-at-purdue.edu} 18, 37 -- CC: {microscopy-at-microscopy.com} 18, 37 -- X-OriginalArrivalTime: 21 Jan 2011 17:51:05.0186 (UTC) FILETIME=[C680EC20:01CBB993] 18, 37 -- Content-Transfer-Encoding: 8bit 18, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LHpw3e030635 ==============================End of - Headers==============================
What is it about your tips that don't function? Are you sure it's not the sample or settings on the tool?
I spent some time last year doing STM with a DI3100 (/Veeco/Bruker) and made many many tips using the side cutter + pull method using Pt/Ir wire. I got some great images from HOPG samples to prove to myself that it was working properly.
The HOPG is highly recommended to get familiar with things (I got my sample from SPI, but Pella also has them). It will guarantee that your sample is not the problem, and allow low-mag imaging (over steps in layers) to very high mag imaging (lattice) to confirm that you have everything working properly.
What worked well for me was I would cut a tip slightly longer than I needed, then held one end with pliers while diagonal cutting/pulling away just the tip at the other end. The direction of cut started nearer the fixed end/pliers and ended up towards the free end. The part that was cut off (stuck to the cutters, flew across the table etc) was junk, the part left gripped in the pliers had a cut/stretched shape but was torn and jagged at the very end (under a microscope).
If you think about the way the sidecutters cut through the metal and plan on having the very end 'torn' you should be able to reliably get excellent tips. I taught the method to a couple of other people and they quickly managed to make similar tips without much trouble.
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
Thanks,
Justin A. Kraft
CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation.
==============================Original Headers============================== 18, 25 -- From cpawlowicz-at-ubmtechinsights.com Fri Jan 21 12:09:20 2011 18, 25 -- Received: from pegasus.semiconductor.com (semi-fw.ubmtechinsights.com [207.61.67.10]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LI9I54015410 18, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 12:09:19 -0600 18, 25 -- Received: from pegasus.semiconductor.com ([192.168.2.10]) by pegasus 18, 25 -- ([192.168.2.10]) with mapi; Fri, 21 Jan 2011 13:09:14 -0500 18, 25 -- From: Chris Pawlowicz {cpawlowicz-at-ubmtechinsights.com} 18, 25 -- To: "'kraftpiano-at-gmail.com'" {kraftpiano-at-gmail.com} 18, 25 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 18, 25 -- Date: Fri, 21 Jan 2011 13:09:13 -0500 18, 25 -- Subject: RE: [Microscopy] STM tip advice. 18, 25 -- Thread-Topic: [Microscopy] STM tip advice. 18, 25 -- Thread-Index: Acu5KiUJYDThOytvR2C07geBayGcHwAabGqw 18, 25 -- Message-ID: {0079587FD46DFC42AC07C9D337C7C895395FD0E387-at-pegasus} 18, 25 -- References: {201101210514.p0L5EufI017085-at-ns.microscopy.com} 18, 25 -- In-Reply-To: {201101210514.p0L5EufI017085-at-ns.microscopy.com} 18, 25 -- Accept-Language: en-US 18, 25 -- Content-Language: en-US 18, 25 -- X-MS-Has-Attach: 18, 25 -- X-MS-TNEF-Correlator: 18, 25 -- acceptlanguage: en-US 18, 25 -- Content-Type: text/plain; charset="us-ascii" 18, 25 -- MIME-Version: 1.0 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LI9I54015410 ==============================End of - Headers==============================
Something is always going on in VP and/or Environmental SEM: http://www.microscopy.org.au/ACMM20/VPSEMWorkshopOutline.pdf And, even a book: http://www.amazon.com/Principles-Practice-Variable-Pressure-Environmental/dp/0470065400
I have attended VP workshops at M&M meetings and I am reading the book.
Know it before you buy it. Our Quanta 400 (100mm stage) Mk1 has a water-cooled thermoelectric cold stage that will permit a specimen no larger than about 7mm. NOTE: PV=NRT is the key when one uses water vapor to pressurize the environment of an hydrated specimen.
Brendan Griffin has, I believe, done his original work on a XL-30, so he might have some suggestions as to what you might do in your deliberations.
My offerings of advice are as follows: 1. W or FEG - depends on requirements for resolution and data acquisition and analysis, because FEG is more expensive to maintain but absolutely necessary for high res imaging work alone. 2. Pressure ranges in VP SEM range from 1 Torr max to 20+ Torr max depending on platform, and therefore, the choice of platform will be based on range of expected uses to which the tool will be put and which gases one may wish to use. 3. Examples of uses we make of VP a. With our fully automated Oxford INCA EDS w/Feature/GSR, I have surveyed a 95 x 95 x 10mm slab of rock for grayscale thresholded ROI's or OOI's (2 sec acquisitions / object identified in a field) for OOI's so that the geologist could determine the area of the rock from which s/he would retrieve a thin section for further survey and probable selection for electron probe analysis (for dating). b. uncoated microfossils for imaging and retrieval. c. Backscatter image montages of large specimens (e.g. up to size in 1) d. There were rumors that at least on user imaged a specimen using H2SO4 vapor. e. Montage maps of large inclusions. 4. Before determining a choice, carefully create a list of expected and projected applications. a. The list defines the tool, if the list is very carefully assembled. 5. ALWAYS make know the total cost of ownership, including personnel, for a decade, and include in purchase as much annual service as possible. 6. Carefully spec the operating systems on the controlling computers with a sure knowledge of what will happen when the OS ceases to be supported, and user interface program will cease being supported by vendor. With our ESEM, the UI lost support before the OS (Win 2k Pro) did. a. My learned approach would be to assure management that THIS part of future support is clearly understood by both the supplier and the purchaser, with clear guarantees by the vendor in the purchase contract. That is, when/if there is a clear glitch in the Vendor's UI, there is a clear guarantee of support for a declared period of time. b. Recognize that each of your instruments may be the only one in the world to manifest a specific problem, and that the vendor's incentive is driven by numbers. c. Require a list of 3rd-party parts of the system you are purchasing. You will want to know how fare in the future your legacy bottlenecks might be. d. Recognize that in my world, at least, a control PC may cost a few to tens of thousands of dollars when replaced by the vendor when there is no contract. Ask what a stage replacement is at the moment, a control PC, a User Interface upgrade, and an estimate of what an upgrade of the disk operating system of the PC and the tool might be in 3-7 years (no vendor wants to give such an estimate, but remember that you have what they want. e. Recognize that you are likely to have any downtime reduced by turning service over to a 3rd party for an instrument under 10 years of age. Our TEM, after 8 years in service is quite similar in all respects to the model that is currently being marketed by FEI. FEI service on everything, and I strongly recommend it. 7. Make a set of the support documentation and parts lists a line item on your specification, and that if part numbers are changed in the future, you will be able to acquire them at no cost (for as long as you own the instrument). 7. The other matter is less obvious. Carefully spec your requirements for HV regulation and stability, in the context of your requirements for stability during an analysis as well as long-term reproducibility. This is difficult, but the HV system quite sophisticated and is repaired by total replacement rather than component replacement.
Finally, when our web site is back on line sometime next week, there are several papers related to VP SEM on the "Links" page that might be of interest to you and your prospective users.
Hope this helps,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Friday, January 21, 2011 10:41 AM To: Monson, Frederick
Hi
I'm not a biologist (most materials science) nor experimented in ESEM (only high vac SEM), but I've on my shelve a thermoelectric module (Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.
Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf, where you can find a great variety of such devices. They give a Dt° in the 70°C range, and are avaible in several dimensions and power. From 10x10 to 62x62 are proposed, but rectanglaire or round shapes too. Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the hot side is low. There is probably room enough to put a heat sink on the stage (copper block), but possibly one would need a water cooled one, what is probably not necessary on the device provided by FEI. If you have the possibilty to build something yourselve, it could be much cheaper. Of coarse without performences waranty !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter. } } You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe. } } Does anyone know if this means the sample can be any size one likes? } } Dave } } -----Original Message----- } X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] } Sent: 19 January 2011 22:53 } To: David Patton } Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu } Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Environmental SEM for Biological Work } } Message: Is there heavy use of ESEM for biological SEM? What is } commonly done on biological ESEM samples? We're looking at } purchasing an environmental SEM and the sales rep. tells us we will } need a Peltier stage to cool the sample to look at wet samples. } Unfortunately, the maximum sample size for the Peltier stage is 3 mm } across. This seems tiny to me for an SEM. Are there many biological } ESEM applications that don't require the sample to be wet? } } Thanks, } } Robin Foley (who spends most of her SEM time looking at metals, } ceramics and polymers!) } } } } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 22 -- From zaluzec-at-microscopy.com Wed Jan 19 16:50:11 2011 } 11, 22 -- Received: from znl.com ([206.69.208.20]) } 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JMoBNn026165 } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 } 11, 22 -- Received: from localhost (localhost [127.0.0.1]) } 11, 22 -- by znl.com (Postfix) with ESMTP id 89D5B316AFA } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 22 -- Received: from znl.com ([127.0.0.1]) } 11, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 22 -- with ESMTP id UF6QAiTpp+Ew for {microscopy-at-microscopy.com} ; } 11, 22 -- Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 22 -- by znl.com (Postfix) with ESMTPA id DBEA8316AEF } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:09 -0600 (CST) } 11, 22 -- Mime-Version: 1.0 } 11, 22 -- Message-Id: {p0624080ec95d1b09d749-at-[206.69.208.22]} } 11, 22 -- Date: Wed, 19 Jan 2011 16:50:07 -0600 } 11, 22 -- To: microscopy-at-microscopy.com } 11, 22 -- From: rfoley-at-uab.edu (by way of MicroscopyListserver) } 11, 22 -- Subject: viaWWW: Environmental SEM for Biological Work } 11, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 48 -- From David.Patton-at-uwe.ac.uk Thu Jan 20 05:52:55 2011 } 23, 48 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1outboundpool.messaging.microsoft.com [157.55.116.155]) } 23, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KBqtKA009538 } 23, 48 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 05:52:55 -0600 } 23, 48 -- Received: from mail131-ch1-R.bigfish.com (216.32.181.172) by } 23, 48 -- CH1EHSOBE007.bigfish.com (10.43.70.57) with Microsoft SMTP Server id } 23, 48 -- 14.1.225.8; Thu, 20 Jan 2011 11:52:54 +0000 } 23, 48 -- Received: from mail131-ch1 (localhost.localdomain [127.0.0.1]) by } 23, 48 -- mail131-ch1-R.bigfish.com (Postfix) with ESMTP id 749AC86020F; Thu, 20 Jan } 23, 48 -- 2011 11:52:54 +0000 (UTC) } 23, 48 -- X-SpamScore: -23 } 23, 48 -- X-BigFish: VPS-23(zz7f0R542N4015L62a3Lzz1202hz31iz8275bhz2fh668h61h) } 23, 48 -- X-Spam-TCS-SCL: 0:0 } 23, 48 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:mailapp03.uwe.ac.uk;RD:mailapp03.uwe.ac.uk;EFVD:NLI } 23, 48 -- Received: from mail131-ch1 (localhost.localdomain [127.0.0.1]) by mail131-ch1 } 23, 48 -- (MessageSwitch) id 129552437448033_30359; Thu, 20 Jan 2011 11:52:54 +0000 } 23, 48 -- (UTC) } 23, 48 -- Received: from CH1EHSMHS030.bigfish.com (snatpool1.int.messaging.microsoft.com } 23, 48 -- [10.43.68.245]) by mail131-ch1.bigfish.com (Postfix) with ESMTP id } 23, 48 -- 0A59516B804E; Thu, 20 Jan 2011 11:52:54 +0000 (UTC) } 23, 48 -- Received: from mailapp03.uwe.ac.uk (164.11.132.65) by CH1EHSMHS030.bigfish.com } 23, 48 -- (10.43.70.30) with Microsoft SMTP Server id 14.1.225.8; Thu, 20 Jan 2011 } 23, 48 -- 11:52:53 +0000 } 23, 48 -- Received: from (egen-hub02.uwe.ac.uk [164.11.251.46]) by mailapp03.uwe.ac.uk } 23, 48 -- with smtp id 7bd0_2fac_cfd14bd6_248b_11e0_8ec4_00142221cca9; Thu, 20 Jan } 23, 48 -- 2011 11:52:52 +0000 } 23, 48 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 48 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.251.46]) with mapi; Thu, 20 Jan 2011 } 23, 48 -- 11:51:21 +0000 } 23, 48 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 48 -- To: "'rfoley-at-uab.edu'" {rfoley-at-uab.edu} } 23, 48 -- Date: Thu, 20 Jan 2011 11:51:20 +0000 } 23, 48 -- Subject: RE: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Topic: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Index: Acu4K66MlRtfppFTQyqwkR6SfbYJwwAa6AKw } 23, 48 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF433F9B96D72-at-EGEN-MBX02.campus.ads.uwe.ac.uk} } 23, 48 -- References: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- In-Reply-To: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- Accept-Language: en-US, en-GB } 23, 48 -- Content-Language: en-US } 23, 48 -- X-MS-Has-Attach: } 23, 48 -- X-MS-TNEF-Correlator: } 23, 48 -- acceptlanguage: en-US, en-GB } 23, 48 -- Content-Type: text/plain; charset="us-ascii" } 23, 48 -- MIME-Version: 1.0 } 23, 48 -- X-OriginatorOrg: uwe.ac.uk } 23, 48 -- Content-Transfer-Encoding: 8bit } 23, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KBqtKA009538 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 21 09:29:38 2011 8, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LFTbmD020182 8, 30 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 09:29:37 -0600 8, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 30 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p0LFTZcQ065634 8, 30 -- ; Fri, 21 Jan 2011 16:29:35 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 8, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8CB321000139; 8, 30 -- Fri, 21 Jan 2011 16:28:58 +0100 (CET) 8, 30 -- Message-ID: {4D39A656.9060606-at-ipcms.u-strasbg.fr} 8, 30 -- Date: Fri, 21 Jan 2011 16:29:26 +0100 8, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 30 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.13) Gecko/20101208 Thunderbird/3.1.7 8, 30 -- MIME-Version: 1.0 8, 30 -- To: microscopy-at-microscopy.com, rfoley-at-uab.edu 8, 30 -- Subject: Re: Environmental SEM for Biological Work 8, 30 -- References: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-IPCMS-MailScanner: Found to be clean 8, 30 -- X-IPCMS-MailScanner-SpamScore: s 8, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 21 Jan 2011 16:29:35 +0100 (CET) 8, 30 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 8, 30 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 8, 30 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From FMonson-at-wcupa.edu Fri Jan 21 13:56:00 2011 25, 37 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LJtx5D007332 25, 37 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 13:55:59 -0600 25, 37 -- X-ASG-Debug-ID: 1295639758-0378c365de9f0a00001-4CH8be 25, 37 -- Received: from WCU-XCH-12.PASSHE.LCL (wcu-xch-12.wcupa.edu [144.26.0.96]) by MX02.WCUPA.EDU with ESMTP id oSATmgMVR7Md9em0; Fri, 21 Jan 2011 14:55:58 -0500 (EST) 25, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 25, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.96 25, 37 -- X-ASG-Whitelist: Client 25, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 25, 37 -- WCU-XCH-12.PASSHE.LCL ([fe80::7112:a4e0:acfd:38d7%15]) with mapi id 25, 37 -- 14.01.0270.001; Fri, 21 Jan 2011 14:55:58 -0500 25, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 25, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 25, 37 -- CC: "jacques.faerber-at-ipcms.u-strasbg.fr" {jacques.faerber-at-ipcms.u-strasbg.fr} , 25, 37 -- "rfoley-at-uab.edu" {rfoley-at-uab.edu} 25, 37 -- Subject: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Topic: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Index: AQHLuYGjxFEkAm5al0SfU4vixxy7lJPbyuuw 25, 37 -- Date: Fri, 21 Jan 2011 19:55:57 +0000 25, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C014C42-at-WCU-XCH-03.PASSHE.LCL} 25, 37 -- References: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- In-Reply-To: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- Accept-Language: en-US 25, 37 -- Content-Language: en-US 25, 37 -- X-MS-Has-Attach: 25, 37 -- X-MS-TNEF-Correlator: 25, 37 -- x-originating-ip: [10.28.57.79] 25, 37 -- Content-Type: text/plain; charset="iso-8859-1" 25, 37 -- MIME-Version: 1.0 25, 37 -- X-Barracuda-Connect: wcu-xch-12.wcupa.edu[144.26.0.96] 25, 37 -- X-Barracuda-Start-Time: 1295639758 25, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 25, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LJtx5D007332 ==============================End of - Headers==============================
Hi Fred, Could you elaborate on item 6e? I'm a little confused.
I like some of your ideas to illuminate the probable life span of a system. Back when dinosaurs roamed the Earth, most people didn't pay much attention to life span because it was (usually correctly) assumed to be long.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: FMonson-at-wcupa.edu [mailto:FMonson-at-wcupa.edu] Sent: Friday, January 21, 2011 2:58 PM To: kenconverse-at-qualityimages.biz
Something is always going on in VP and/or Environmental SEM: http://www.microscopy.org.au/ACMM20/VPSEMWorkshopOutline.pdf And, even a book: http://www.amazon.com/Principles-Practice-Variable-Pressure-Environmental/dp /0470065400
I have attended VP workshops at M&M meetings and I am reading the book.
Know it before you buy it. Our Quanta 400 (100mm stage) Mk1 has a water-cooled thermoelectric cold stage that will permit a specimen no larger than about 7mm. NOTE: PV=NRT is the key when one uses water vapor to pressurize the environment of an hydrated specimen.
Brendan Griffin has, I believe, done his original work on a XL-30, so he might have some suggestions as to what you might do in your deliberations.
My offerings of advice are as follows: 1. W or FEG - depends on requirements for resolution and data acquisition and analysis, because FEG is more expensive to maintain but absolutely necessary for high res imaging work alone. 2. Pressure ranges in VP SEM range from 1 Torr max to 20+ Torr max depending on platform, and therefore, the choice of platform will be based on range of expected uses to which the tool will be put and which gases one may wish to use. 3. Examples of uses we make of VP a. With our fully automated Oxford INCA EDS w/Feature/GSR, I have surveyed a 95 x 95 x 10mm slab of rock for grayscale thresholded ROI's or OOI's (2 sec acquisitions / object identified in a field) for OOI's so that the geologist could determine the area of the rock from which s/he would retrieve a thin section for further survey and probable selection for electron probe analysis (for dating). b. uncoated microfossils for imaging and retrieval. c. Backscatter image montages of large specimens (e.g. up to size in 1) d. There were rumors that at least on user imaged a specimen using H2SO4 vapor. e. Montage maps of large inclusions. 4. Before determining a choice, carefully create a list of expected and projected applications. a. The list defines the tool, if the list is very carefully assembled. 5. ALWAYS make know the total cost of ownership, including personnel, for a decade, and include in purchase as much annual service as possible. 6. Carefully spec the operating systems on the controlling computers with a sure knowledge of what will happen when the OS ceases to be supported, and user interface program will cease being supported by vendor. With our ESEM, the UI lost support before the OS (Win 2k Pro) did. a. My learned approach would be to assure management that THIS part of future support is clearly understood by both the supplier and the purchaser, with clear guarantees by the vendor in the purchase contract. That is, when/if there is a clear glitch in the Vendor's UI, there is a clear guarantee of support for a declared period of time. b. Recognize that each of your instruments may be the only one in the world to manifest a specific problem, and that the vendor's incentive is driven by numbers. c. Require a list of 3rd-party parts of the system you are purchasing. You will want to know how fare in the future your legacy bottlenecks might be. d. Recognize that in my world, at least, a control PC may cost a few to tens of thousands of dollars when replaced by the vendor when there is no contract. Ask what a stage replacement is at the moment, a control PC, a User Interface upgrade, and an estimate of what an upgrade of the disk operating system of the PC and the tool might be in 3-7 years (no vendor wants to give such an estimate, but remember that you have what they want. e. Recognize that you are likely to have any downtime reduced by turning service over to a 3rd party for an instrument under 10 years of age. Our TEM, after 8 years in service is quite similar in all respects to the model that is currently being marketed by FEI. FEI service on everything, and I strongly recommend it. 7. Make a set of the support documentation and parts lists a line item on your specification, and that if part numbers are changed in the future, you will be able to acquire them at no cost (for as long as you own the instrument). 7. The other matter is less obvious. Carefully spec your requirements for HV regulation and stability, in the context of your requirements for stability during an analysis as well as long-term reproducibility. This is difficult, but the HV system quite sophisticated and is repaired by total replacement rather than component replacement.
Finally, when our web site is back on line sometime next week, there are several papers related to VP SEM on the "Links" page that might be of interest to you and your prospective users.
Hope this helps,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr] Sent: Friday, January 21, 2011 10:41 AM To: Monson, Frederick
Hi
I'm not a biologist (most materials science) nor experimented in ESEM (only high vac SEM), but I've on my shelve a thermoelectric module (Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.
Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf, where you can find a great variety of such devices. They give a Dt° in the 70°C range, and are avaible in several dimensions and power. From 10x10 to 62x62 are proposed, but rectanglaire or round shapes too. Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the hot side is low. There is probably room enough to put a heat sink on the stage (copper block), but possibly one would need a water cooled one, what is probably not necessary on the device provided by FEI. If you have the possibilty to build something yourselve, it could be much cheaper. Of coarse without performences waranty !
Hope it helps
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter. } } You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe. } } Does anyone know if this means the sample can be any size one likes? } } Dave } } -----Original Message----- } X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu] } Sent: 19 January 2011 22:53 } To: David Patton } Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu } Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Environmental SEM for Biological Work } } Message: Is there heavy use of ESEM for biological SEM? What is } commonly done on biological ESEM samples? We're looking at } purchasing an environmental SEM and the sales rep. tells us we will } need a Peltier stage to cool the sample to look at wet samples. } Unfortunately, the maximum sample size for the Peltier stage is 3 mm } across. This seems tiny to me for an SEM. Are there many biological } ESEM applications that don't require the sample to be wet? } } Thanks, } } Robin Foley (who spends most of her SEM time looking at metals, } ceramics and polymers!) } } } } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } ==============================Original Headers============================== } 11, 22 -- From zaluzec-at-microscopy.com Wed Jan 19 16:50:11 2011 } 11, 22 -- Received: from znl.com ([206.69.208.20]) } 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0JMoBNn026165 } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 } 11, 22 -- Received: from localhost (localhost [127.0.0.1]) } 11, 22 -- by znl.com (Postfix) with ESMTP id 89D5B316AFA } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 22 -- Received: from znl.com ([127.0.0.1]) } 11, 22 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 22 -- with ESMTP id UF6QAiTpp+Ew for {microscopy-at-microscopy.com} ; } 11, 22 -- Wed, 19 Jan 2011 16:50:11 -0600 (CST) } 11, 22 -- Received: from [206.69.208.22] (mac22.zaluzec.com [206.69.208.22]) } 11, 22 -- by znl.com (Postfix) with ESMTPA id DBEA8316AEF } 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Jan 2011 16:50:09 -0600 (CST) } 11, 22 -- Mime-Version: 1.0 } 11, 22 -- Message-Id: {p0624080ec95d1b09d749-at-[206.69.208.22]} } 11, 22 -- Date: Wed, 19 Jan 2011 16:50:07 -0600 } 11, 22 -- To: microscopy-at-microscopy.com } 11, 22 -- From: rfoley-at-uab.edu (by way of MicroscopyListserver) } 11, 22 -- Subject: viaWWW: Environmental SEM for Biological Work } 11, 22 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 48 -- From David.Patton-at-uwe.ac.uk Thu Jan 20 05:52:55 2011 } 23, 48 -- Received: from ch1outboundpool.messaging.microsoft.com (ch1outboundpool.messaging.microsoft.com [157.55.116.155]) } 23, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0KBqtKA009538 } 23, 48 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 05:52:55 -0600 } 23, 48 -- Received: from mail131-ch1-R.bigfish.com (216.32.181.172) by } 23, 48 -- CH1EHSOBE007.bigfish.com (10.43.70.57) with Microsoft SMTP Server id } 23, 48 -- 14.1.225.8; Thu, 20 Jan 2011 11:52:54 +0000 } 23, 48 -- Received: from mail131-ch1 (localhost.localdomain [127.0.0.1]) by } 23, 48 -- mail131-ch1-R.bigfish.com (Postfix) with ESMTP id 749AC86020F; Thu, 20 Jan } 23, 48 -- 2011 11:52:54 +0000 (UTC) } 23, 48 -- X-SpamScore: -23 } 23, 48 -- X-BigFish: VPS-23(zz7f0R542N4015L62a3Lzz1202hz31iz8275bhz2fh668h61h) } 23, 48 -- X-Spam-TCS-SCL: 0:0 } 23, 48 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:mailapp03.uwe.ac.uk;RD:mailapp03.uwe.ac.uk; EFVD:NLI } 23, 48 -- Received: from mail131-ch1 (localhost.localdomain [127.0.0.1]) by mail131-ch1 } 23, 48 -- (MessageSwitch) id 129552437448033_30359; Thu, 20 Jan 2011 11:52:54 +0000 } 23, 48 -- (UTC) } 23, 48 -- Received: from CH1EHSMHS030.bigfish.com (snatpool1.int.messaging.microsoft.com } 23, 48 -- [10.43.68.245]) by mail131-ch1.bigfish.com (Postfix) with ESMTP id } 23, 48 -- 0A59516B804E; Thu, 20 Jan 2011 11:52:54 +0000 (UTC) } 23, 48 -- Received: from mailapp03.uwe.ac.uk (164.11.132.65) by CH1EHSMHS030.bigfish.com } 23, 48 -- (10.43.70.30) with Microsoft SMTP Server id 14.1.225.8; Thu, 20 Jan 2011 } 23, 48 -- 11:52:53 +0000 } 23, 48 -- Received: from (egen-hub02.uwe.ac.uk [164.11.251.46]) by mailapp03.uwe.ac.uk } 23, 48 -- with smtp id 7bd0_2fac_cfd14bd6_248b_11e0_8ec4_00142221cca9; Thu, 20 Jan } 23, 48 -- 2011 11:52:52 +0000 } 23, 48 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 48 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.251.46]) with mapi; Thu, 20 Jan 2011 } 23, 48 -- 11:51:21 +0000 } 23, 48 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 48 -- To: "'rfoley-at-uab.edu'" {rfoley-at-uab.edu} } 23, 48 -- Date: Thu, 20 Jan 2011 11:51:20 +0000 } 23, 48 -- Subject: RE: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Topic: [Microscopy] viaWWW: Environmental SEM for Biological Work } 23, 48 -- Thread-Index: Acu4K66MlRtfppFTQyqwkR6SfbYJwwAa6AKw } 23, 48 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF433F9B96D72-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 48 -- References: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- In-Reply-To: {201101192253.p0JMrH9m031418-at-ns.microscopy.com} } 23, 48 -- Accept-Language: en-US, en-GB } 23, 48 -- Content-Language: en-US } 23, 48 -- X-MS-Has-Attach: } 23, 48 -- X-MS-TNEF-Correlator: } 23, 48 -- acceptlanguage: en-US, en-GB } 23, 48 -- Content-Type: text/plain; charset="us-ascii" } 23, 48 -- MIME-Version: 1.0 } 23, 48 -- X-OriginatorOrg: uwe.ac.uk } 23, 48 -- Content-Transfer-Encoding: 8bit } 23, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0KBqtKA009538 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 30 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jan 21 09:29:38 2011 8, 30 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LFTbmD020182 8, 30 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 09:29:37 -0600 8, 30 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 8, 30 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p0LFTZcQ065634 8, 30 -- ; Fri, 21 Jan 2011 16:29:35 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 8, 30 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 8, 30 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 8CB321000139; 8, 30 -- Fri, 21 Jan 2011 16:28:58 +0100 (CET) 8, 30 -- Message-ID: {4D39A656.9060606-at-ipcms.u-strasbg.fr} 8, 30 -- Date: Fri, 21 Jan 2011 16:29:26 +0100 8, 30 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 8, 30 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.13) Gecko/20101208 Thunderbird/3.1.7 8, 30 -- MIME-Version: 1.0 8, 30 -- To: microscopy-at-microscopy.com, rfoley-at-uab.edu 8, 30 -- Subject: Re: Environmental SEM for Biological Work 8, 30 -- References: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- In-Reply-To: {201101201202.p0KC2MDY016523-at-ns.microscopy.com} 8, 30 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 30 -- Content-Transfer-Encoding: 8bit 8, 30 -- X-IPCMS-MailScanner: Found to be clean 8, 30 -- X-IPCMS-MailScanner-SpamScore: s 8, 30 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 8, 30 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 21 Jan 2011 16:29:35 +0100 (CET) 8, 30 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 8, 30 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 8, 30 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
==============================Original Headers============================== 25, 37 -- From FMonson-at-wcupa.edu Fri Jan 21 13:56:00 2011 25, 37 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 25, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LJtx5D007332 25, 37 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 13:55:59 -0600 25, 37 -- X-ASG-Debug-ID: 1295639758-0378c365de9f0a00001-4CH8be 25, 37 -- Received: from WCU-XCH-12.PASSHE.LCL (wcu-xch-12.wcupa.edu [144.26.0.96]) by MX02.WCUPA.EDU with ESMTP id oSATmgMVR7Md9em0; Fri, 21 Jan 2011 14:55:58 -0500 (EST) 25, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 25, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.96 25, 37 -- X-ASG-Whitelist: Client 25, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 25, 37 -- WCU-XCH-12.PASSHE.LCL ([fe80::7112:a4e0:acfd:38d7%15]) with mapi id 25, 37 -- 14.01.0270.001; Fri, 21 Jan 2011 14:55:58 -0500 25, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 25, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 25, 37 -- CC: "jacques.faerber-at-ipcms.u-strasbg.fr" {jacques.faerber-at-ipcms.u-strasbg.fr} , 25, 37 -- "rfoley-at-uab.edu" {rfoley-at-uab.edu} 25, 37 -- Subject: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Topic: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Environmental SEM for Biological Work 25, 37 -- Thread-Index: AQHLuYGjxFEkAm5al0SfU4vixxy7lJPbyuuw 25, 37 -- Date: Fri, 21 Jan 2011 19:55:57 +0000 25, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C014C42-at-WCU-XCH-03.PASSHE.LCL} 25, 37 -- References: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- In-Reply-To: {201101211541.p0LFfEBr000930-at-ns.microscopy.com} 25, 37 -- Accept-Language: en-US 25, 37 -- Content-Language: en-US 25, 37 -- X-MS-Has-Attach: 25, 37 -- X-MS-TNEF-Correlator: 25, 37 -- x-originating-ip: [10.28.57.79] 25, 37 -- Content-Type: text/plain; charset="iso-8859-1" 25, 37 -- MIME-Version: 1.0 25, 37 -- X-Barracuda-Connect: wcu-xch-12.wcupa.edu[144.26.0.96] 25, 37 -- X-Barracuda-Start-Time: 1295639758 25, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 25, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 25, 37 -- Content-Transfer-Encoding: 8bit 25, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LJtx5D007332 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 28 -- From kenconverse-at-qualityimages.biz Fri Jan 21 15:16:34 2011 38, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.122]) 38, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0LLGYDY024815 38, 28 -- for {microscopy-at-microscopy.com} ; Fri, 21 Jan 2011 15:16:34 -0600 38, 28 -- X-Authority-Analysis: v=1.1 cv=3jtQBdTzPyV+fq4oCU/u8ZPrJJGN11HvhaDVxyWhycI= c=1 sm=0 a=cCsHsa71OjYA:10 a=8nJEP1OIZ-IA:10 a=o/BqynnMLxMJiB4/byRFHg==:17 a=Zx37jsudAAAA:8 a=S-ikiy0eAAAA:8 a=vggBfdFIAAAA:8 a=8Wz4FX-BAAAA:8 a=SsN2on13AAAA:8 a=-gyhParZAAAA:8 a=pzmDz54eAAAA:8 a=jU2bWg6BAAAA:8 a=yMhMjlubAAAA:8 a=hCCmtxs2AAAA:8 a=F59vpjFj9DikHUfwRyUA:9 a=eUjghLitqcq2qX_Fj8IA:7 a=x3T5Eb86TmGMBu5YkpUYE0nMdL0A:4 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=fM1NKZWFy18A:10 a=7abxOcK5BwwA:10 a=BkF1guiUBFgA:10 a=RyjLG6jaqdoA:10 a=losLsr3N_5UA:10 a=k6cFIQMUckkA:10 a=3_2eAsHTqJoA:10 a=bsRvvyHUn3IA:10 a=G91thX30KR8A:10 a=UPymgFs0m_tAJC2f:21 a=gBBtCaMGKSruqQVW:21 a=o/BqynnMLxMJiB4/byRFHg==:117 38, 28 -- X-Cloudmark-Score: 0 38, 28 -- X-Originating-IP: 72.227.97.248 38, 28 -- Received: from [72.227.97.248] ([72.227.97.248:2040] helo=Ken) 38, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 38, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 38, 28 -- id F9/7B-02631-1B7F93D4; Fri, 21 Jan 2011 21:16:34 +0000 38, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 38, 28 -- To: {FMonson-at-wcupa.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 38, 28 -- Subject: RE: [Microscopy] Environmental SEM for Biological Work 38, 28 -- Date: Fri, 21 Jan 2011 16:16:24 -0500 38, 28 -- Message-ID: {5CD9202EC2384D55BFE10F4932BF4D43-at-Ken} 38, 28 -- MIME-Version: 1.0 38, 28 -- Content-Type: text/plain; 38, 28 -- charset="iso-8859-1" 38, 28 -- X-Priority: 3 (Normal) 38, 28 -- X-MSMail-Priority: Normal 38, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 38, 28 -- Importance: Normal 38, 28 -- Thread-Index: Acu5pY5m+X84NQPuSFy3Y1W6iY1t2QACmmYg 38, 28 -- In-Reply-To: {201101211958.p0LJwKwM010950-at-ns.microscopy.com} 38, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 38, 28 -- Content-Transfer-Encoding: 8bit 38, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0LLGYDY024815 ==============================End of - Headers==============================
Jun asked for a calibration grid with pitch near 0.1 um. My company makes and sells two relevant products: -a 70-nm pitch 1-dimensional pattern (lines and spaces) and -a 144-nm pitch square grid. These are available as ordinary calibration specimens or as standards traceable to the international meter. The traceable standards come with a test report that shows both the pitch value and its uncertainty. Please find further details at: http://www.asmicro.com/Supplies/utc.htm - traceable calibration specimens.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] 2009-2010 is our 20th year in business! =============================================
----- Original Message ----- From: junhe1970-at-gmail.com To: donc-at-asmicro.com Sent: Tuesday, December 21, 2010 8:05 AM Subject: [a] [Microscopy] viaWWW: SEM stardard grid
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both junhe1970-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: junhe1970-at-gmail.com Name: jun
Title-Subject: [Filtered] SEM stardard grid
Message: We frequently uses SEM ( Hitachi 4700, FE) to measure some wafer cross-section features. The measurement result is sometimes off the mark . I wonder if some one here could recommend some fine grid (0.1 um)standard Jun
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-med.wayne.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Wayne State Univ. School of Medicine
Title-Subject: [Filtered] Post-embedding/sectioning FluoroNanogold Labeling for TEM
Message: Hello, Some of our antigens are difficult to label in mammalian fat cells and muscles, such as particular lipid droplet proteins. I have tried several methods of antigen unmasking and probe maintenance in post-sectioning fluoronanogold labeling, including absence of uranyl acetate in the LR-White embedding medium, reduction of osmium post-fixation concentration to reduce etching propensity of silver-enhanced gold probes, antigen unmasking with oxidizing (sodium metaperiodate) and reducing (sodium borohydride) agents and Tris base pH 10. I have reduced my glutaraldehyde concentration to 0.10% and maintained paraformaldehyde at 2%.
Pre-embedding fluoronanogold labeling appears to work with the absence of glutaraldehyde in the fixative, however the ultrastructure is quite compromised, as one would expect by TEM.
I have been thinking now of using LR-Gold, until I hear from some experts on any other suggestions. Thank you very much! Vickie
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.knowles-at-ucl.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.knowles-at-ucl.ac.uk Name: Jonathan Knowles
Organization: UCL
Title-Subject: [Filtered] Electronics side of an EDX system
Message: Hi
We have inherited an EDX system which seems to work OK (sorry I don't have details to hand but wanted to ask this before I forgot!). The system works OK, but the electronics side is really ancient (It has a microVAX type drive with about 20Mb storage to run the OS and software) and am concerned that it might die some time. I wondered if anyone had ever built some more up to date hardware/interface? The connections for the EDX consist of a BNC for the HT and then a 9 pin connector presumably for data.
I am running a LEO1450VP SEM and encounter a problem related to the use of the mouse. The middle button of a 3-key mouse is used extremely in LEO32 software and suddenly the software disable the use of the middle button.
I am able to use both left and right button, except the middle one. I also tried to edit the toolbar to set the middle button’s function. However, both the “OK” and “Apply” buttons were deactivated so the settings I made were useless.
If anyone has similar experiences, please kindly contact me. Thank you very much.
Sean EMC -at- Saint Mary’s University Halifax, NS xyang-at-smu.ca
==============================Original Headers============================== 8, 23 -- From xyang-at-SMU.CA Mon Jan 24 13:12:57 2011 8, 23 -- Received: from HUSKY0.SMU.CA (Husky0.smu.ca [140.184.1.100]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0OJCuv1004741 8, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Jan 2011 13:12:57 -0600 8, 23 -- Received: from s422XY ("port 4882"-at-[140.184.128.189]) 8, 23 -- by HUSKY1.SMU.CA (PMDF V6.3-x13 #31544) 8, 23 -- with ESMTP id {01NX08EKYK4Y8XENI6-at-HUSKY1.SMU.CA} for 8, 23 -- Microscopy-at-microscopy.com; Mon, 24 Jan 2011 15:12:56 -0400 8, 23 -- Date: Mon, 24 Jan 2011 15:12:56 -0400 8, 23 -- From: Xiang Yang {xyang-at-SMU.CA} 8, 23 -- Subject: RE: middle mouse button problem in LEO SEM system 8, 23 -- In-reply-to: 8, 23 -- To: Microscopy-at-microscopy.com 8, 23 -- Reply-to: xyang-at-SMU.CA 8, 23 -- Message-id: {00a901cbbbfa$b4e166c0$1ea43440$-at-ca} 8, 23 -- MIME-version: 1.0 8, 23 -- X-Mailer: Microsoft Office Outlook 12.0 8, 23 -- Content-type: text/plain; charset=iso-8859-1 8, 23 -- Content-language: en-us 8, 23 -- Thread-Index: Acu7+lbpBvPk7hmmQoS04MHTTVYCrgAADeYA 8, 23 -- References: 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0OJCuv1004741 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reynolds-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reynolds-at-vt.edu Name: Bill Reynolds
Organization: Virginia Tech
Title-Subject: [Filtered] Fwd: EDS line scans and time on my hands
Message: This comment does not directly address Frank Karl's original question about pixel size and measurement spacing, but it relates to the problem of detecting small changes in boundary concentrations.
Measuring grain boundary concentrations from EDS line scans is problematic because the electron beam geometry is always convoluted with the solute distribution in the sample. To determine, say, the maximum solute concentration at a grain boundary, you have to know the extent of beam broadening in the sample AND have an accurate model for how the solute is distributed across the boundary. If you don't already know where the solute is, you have to make an educated guess. For example, you might assume the solute is confined to a boundary region 1 nm thick so you can back-out a boundary concentration from the measured EDS profile, and the probe size with broadening. That involves a lot of assumptions and uncertainties.
A clever alternate strategy is to make an integrated measurement across a boundary to determine the total excess solute associated with the boundary (the excess solute is the amount of solute per unit area of grain boundary). This quantity is calculated from the difference between the amount of solute found in a sample volume that includes the boundary and the amount of solute found in an equivalent volume without the boundary (but located nearby).
The excess solute is not a concentration, and it tells you nothing about the solute distribution at a boundary, but it has some distinct advantages when trying to quantify solute enrichment or depletion at a boundary: it can be measured without knowing much about the probe size, beam broadening, or boundary structure, and you do not need to assume anything about the solute profile. Because the method employs a scanned box that straddles a boundary, it has better counting statistics than single line scans, and that makes it pretty good at detecting small solute enrichments.
The technique was developed with STEM in mind, but I don't see any reason why it cannot be used for EDS measurements in an SEM. Here are some example investigations that used the method: J.A.S. Ikeda, Y.-M. Chiang, and C.G. Madras: Ceram. Trans., 1991, vol. 24, pp. 341-348 A. J. Garratt-Reed: Proceedings of the 50th Annual Meeting, EMSA, 1992, p. 1206-1207 H.A. Fletcher, et al.: Scripta Mater., 2001, vol. 45 (5), pp. 561-567 E. S. Humphreys, et al. Metall. Mat. Trans A. vol 35A (4), 2004, pp 1223-1235.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbarlow-at-sciences.sdsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: EM Facility/San Diego State U Biology
Title-Subject: [Filtered] Uninterruptible power supply
Message: I am looking for a ups system for my Quanta 450 FESEM. If anyone has any experiences with various models, I would appreciate hearing about it.
A good source of knowledge for these thing is your IT department. Servers, especially those used in high power computing, tends to consume a lot of current and tend to fail badly when the power is interrupted.
cheers, logo
Gabriel Lapointe, M.Sc. gabriel.lapointe2-at-gmail.com http://gabriellapointe.ca
logo
On Mon, 2011-01-24 at 14:11 -0600, sbarlow-at-sciences.sdsu.edu wrote:
} Email: sbarlow-at-sciences.sdsu.edu } Name: Steve Barlow } } Organization: EM Facility/San Diego State U Biology } } Title-Subject: [Filtered] Uninterruptible power supply } } Message: I am looking for a ups system for my Quanta 450 FESEM. If } anyone has any experiences with various models, I would appreciate } hearing about it. } } The needed characteristics are: } Rating= 8 kVA/6.4 kW } Input voltage= 172-285 Vac } Input frequency= 40-70 Hz } Output voltage= 230 Vac } Output frequency= 50/60 Hz } } Thanks in advance. Vendors may contact me directly offline } } Steve } } Login Host: 146.244.234.42
==============================Original Headers============================== 16, 39 -- From gabriel.lapointe2-at-gmail.com Mon Jan 24 14:42:48 2011 16, 39 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 16, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0OKglYZ025568 16, 39 -- for {microscopy-at-microscopy.com} ; Mon, 24 Jan 2011 14:42:48 -0600 16, 39 -- Received: by vws10 with SMTP id 10so1943214vws.0 16, 39 -- for {microscopy-at-microscopy.com} ; Mon, 24 Jan 2011 12:42:47 -0800 (PST) 16, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 39 -- d=gmail.com; s=gamma; 16, 39 -- h=domainkey-signature:subject:from:to:in-reply-to:references 16, 39 -- :content-type:date:message-id:mime-version:x-mailer 16, 39 -- :content-transfer-encoding; 16, 39 -- bh=GVfBaQ+VCiRNLFcxJ0wPaQeKxFqy14XAt71oALhycsQ=; 16, 39 -- b=eRU8y3z70CsQ/rhz4BTxCxBnBZZ8lTDsIqi/oXUs6zF9n+kFyt4ep1wQ2ISrcW940d 16, 39 -- u1cE9IWZ8GDYp6SdwCDHmo1FM47aYx3heZkDaC3gkyRNDDtNNTh5dVN9V/5rQrabEPBF 16, 39 -- btm6oJe0eyYNeSaV/o4LYMd7KL5Sf6ywHmf8k= 16, 39 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 16, 39 -- d=gmail.com; s=gamma; 16, 39 -- h=subject:from:to:in-reply-to:references:content-type:date:message-id 16, 39 -- :mime-version:x-mailer:content-transfer-encoding; 16, 39 -- b=Bo0N/IclnOp4bEfaXhnspBRVdDeV17yeqoCn1mGlu7XT/+orl4wd0CdgpwyCtBvYeg 16, 39 -- mtTjg9ydLQfx7guPDrH/1EWJkHOagEeO/hT+u4XkrP2Lz/c6E657sWChFJU0JKKnbMe4 16, 39 -- BCazhlDq/lbSUQdNdybh5nMqDIqqZoVFdHlkw= 16, 39 -- Received: by 10.220.43.135 with SMTP id w7mr1259708vce.233.1295901765696; 16, 39 -- Mon, 24 Jan 2011 12:42:45 -0800 (PST) 16, 39 -- Received: from [132.204.85.122] (d-132-204-85-122.d-fac.umontreal.ca [132.204.85.122]) 16, 39 -- by mx.google.com with ESMTPS id p8sm4270212vcr.18.2011.01.24.12.42.44 16, 39 -- (version=SSLv3 cipher=RC4-MD5); 16, 39 -- Mon, 24 Jan 2011 12:42:45 -0800 (PST) 16, 39 -- Subject: Re: [Microscopy] viaWWW: Uninterruptible power supply 16, 39 -- From: Gabriel Lapointe {gabriel.lapointe2-at-gmail.com} 16, 39 -- To: microscopy-at-microscopy.com 16, 39 -- In-Reply-To: {201101242011.p0OKBX9c018428-at-ns.microscopy.com} 16, 39 -- References: {201101242011.p0OKBX9c018428-at-ns.microscopy.com} 16, 39 -- Content-Type: text/plain; charset="UTF-8" 16, 39 -- Date: Mon, 24 Jan 2011 15:42:42 -0500 16, 39 -- Message-ID: {1295901762.2679.10.camel-at-p0782954} 16, 39 -- Mime-Version: 1.0 16, 39 -- X-Mailer: Evolution 2.28.3 16, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gul417-at-mail.usask.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
The insulator cable in our aged Edwards S150B sputter coater broke (so no HT) a few days ago. Does anyone have a clue on where can I order this part (or a compatible part from other makes) or get a service (I'm in Canada)?
We are recruiting a number of posts here in Oxford (UK), several of which might be of particular interest to readers here. If you know anyone who might be interested in these posts please do forward them this info. The closing dates are all Feb 3rd.
Micron Oxford is funded by a major grant from the Wellcome Trust to the Department of Biochemistry and the Sir William Dunn School of Pathology in association with the Wellcome Trust Centre for Human Genetics and The Oxford Center for Integrative Systems Biology. Its aim is to establish state of the art light and electron microscopy facilities located in the heart of the Oxford University research community with an emphasis on technology development.
We are currently trying to recruit an Assistant Facility Manager, a Correlative Light/Electron Microscopy Specialist and an Image Analysis Specialist. Full details can be found on our website http://www.micronoxford.com/
Additionally we are looking to appoint a Group Leader with expertise in the development or application of advanced imaging techniques or the modulation of protein function with a background in physics, chemistry or biology. We are also looking to appoint at senior post-doctoral level a system administrator specialising in the area of hierarchical data storage development/management. Full particulars of each post can be found at the Micron website (http://www.micronoxford.com/).
Thanks,
Ian -- Ian Dobbie Micron Imaging Facility Manager, Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU Tel: +44 (0)1865 613323 Email: ian.dobbie-at-bioch.ox.ac.uk
==============================Original Headers============================== 7, 22 -- From ian.dobbie-at-bioch.ox.ac.uk Tue Jan 25 07:26:31 2011 7, 22 -- Received: from relay9.mail.ox.ac.uk (relay9.mail.ox.ac.uk [163.1.2.169]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PDQVUT021373 7, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 07:26:31 -0600 7, 22 -- Received: from smtp2.mail.ox.ac.uk ([163.1.2.205]) 7, 22 -- by relay9.mail.ox.ac.uk with esmtp (Exim 4.71) 7, 22 -- (envelope-from {ian.dobbie-at-bioch.ox.ac.uk} ) 7, 22 -- id 1Phiuk-000891-VL 7, 22 -- for Microscopy-at-microscopy.com; Tue, 25 Jan 2011 13:26:30 +0000 7, 22 -- Received: from vpn6184.bioch.ox.ac.uk ([129.67.76.184] helo=oisins-macbook.home) 7, 22 -- by smtp2.mail.ox.ac.uk with esmtp (Exim 4.69) 7, 22 -- (envelope-from {ian.dobbie-at-bioch.ox.ac.uk} ) 7, 22 -- id 1Phiuk-00084f-7i 7, 22 -- for Microscopy-at-microscopy.com; Tue, 25 Jan 2011 13:26:30 +0000 7, 22 -- From: Ian Dobbie {ian.dobbie-at-bioch.ox.ac.uk} 7, 22 -- To: Microscopy-at-microscopy.com 7, 22 -- Subject: Recruiting correlative light/EM specialist, assistant facility manager and image analysis specialist. 7, 22 -- Date: Tue, 25 Jan 2011 13:25:28 +0000 7, 22 -- Message-ID: {g38vy96rnd.fsf-at-oisins-macbook.home} 7, 22 -- User-Agent: Gnus/5.110011 (No Gnus v0.11) Emacs/22.3 (darwin) 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: text/plain; charset=us-ascii ==============================End of - Headers==============================
Organization: Wayne State Univ. School of Medicine
Title-Subject: [Filtered] Post-embedding/sectioning FluoroNanogold Labeling for TEM
Message: Hello, Some of our antigens are difficult to label in mammalian fat cells and muscles, such as particular lipid droplet proteins. I have tried several methods of antigen unmasking and probe maintenance in post-sectioning fluoronanogold labeling, including absence of uranyl acetate in the LR-White embedding medium, reduction of osmium post-fixation concentration to reduce etching propensity of silver-enhanced gold probes, antigen unmasking with oxidizing (sodium metaperiodate) and reducing (sodium borohydride) agents and Tris base pH 10. I have reduced my glutaraldehyde concentration to 0.10% and maintained paraformaldehyde at 2%.
Pre-embedding fluoronanogold labeling appears to work with the absence of glutaraldehyde in the fixative, however the ultrastructure is quite compromised, as one would expect by TEM.
I have been thinking now of using LR-Gold, until I hear from some experts on any other suggestions. Thank you very much! Vickie
Hi Vickie,
I had a similar situation and had successfully used pre-embed labelling with FluoroNanogold. I hope I can help. In the post-section fluoronanogold labelling, there might not be enough cross linking of your proteins to keep them stabilized especially during the dehydration and embedding steps. Antigen unmasking might not be the issue there but rather the proteins have been extracted. From what I recall lipids rely on osmium tetroxide and urany acetate fixation. Assuming you have abundant amount of these proteins, if you have already tried the gradual low temperature approach during dehydration and resin infiltration steps and there is still no labelling, I would try another approach.
You mentioned that pre-embed fluoronanogold labelling worked but the ultrastructure quality was quite compromised. That sounds promising when you get similar labelling pattern as in previous light microscopy data. In this case, you might be able to fine tune the fixation step and permeabilization if the latter was used. In my experience, this approach worked better for the Flower, a synaptic vesicle protein in the neuromuscular junction (NMJ) which we were working on. We tried several fixation combinations but found that overnight 4% paraformaldehyde fixation achieved sufficient labelling. Although ultrastructure quality was not the same as routine TEM, the organelles were still recognizable. We were a bit lucky because the NMJs lie close to the muscle surface and go only as deep as 5-8 microns into the tissue. Also, we worked on fillet-dissected third instar Drosophila larvae and did not need 50 micron thick vivatome sections. Even so, we still needed permeabilization steps but not as drastic as used in regular immunofluorescence. After trying different detergents and conc, we added 0.01% Tween 20 in the blocking step. The nice thing about using FluoroNanogold is we can examine the fluorescence labelling before proceeding to next steps. In addition, you can post fix with 3% Glut and use lower concentration of osmium tetroxide prior to dehydration and embedding so the sample quality should be more enhanced.
I am not an expert in Immunocytochemistry like Drs Paul Webster and Jan Leunissen and others out there but one thing I learned as I was getting into this is that there is no single labelling approach. Pre-embed method is not suitable for all samples but would likely work on cell monolayers/suspensions, vivatome sections and samples where antigen is easily accessible. We try to fit the best immuno-EM method to your samples which we all know usually takes time. I hope you have plenty of time to optimize the method for your samples.
I will send a pdf of the Flower paper so that you can check the micrographs. A detailed pre-embed method with Fluoronanogold is on the supplemental material.
Good luck,
Claire
------------------------------------------------------------ Claire M. Haueter Res. Tech. II (Electron Microscopy) Bellen Lab HHMI-Baylor College of Medicine Jan and Dan Duncan Neurological Research Institute 1250 Moursund St. Suite 1125 Mailstop NR-1125 Houston, TX 77030 Phone: 832-824-8772 Fax: 832-825-1240 Email: chaueter-at-bcm.edu -------------------------------------------------------------
==============================Original Headers============================== 2, 31 -- From chaueter-at-bcm.edu Tue Jan 25 11:20:03 2011 2, 31 -- Received: from iron1.corp.bcm.tmc.edu (iron1.corp.bcm.tmc.edu [128.249.224.13]) 2, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PHK2ZE013593 2, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 11:20:03 -0600 2, 31 -- X-IronPort-AV: E=Sophos;i="4.60,375,1291615200"; 2, 31 -- d="scan'208";a="106861743" 2, 31 -- Received: from unknown (HELO EPW-EXHUB3-P022.ad.bcm.edu) ([10.22.132.49]) 2, 31 -- by iron1-out.bcm.edu with ESMTP; 25 Jan 2011 11:20:02 -0600 2, 31 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 2, 31 -- EPW-EXHUB3-P022.ad.bcm.edu ([::1]) with mapi; Tue, 25 Jan 2011 11:20:02 -0600 2, 31 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 2, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 31 -- Date: Tue, 25 Jan 2011 11:20:02 -0600 2, 31 -- Subject: Reply on Post-embedding/sectioning FluoroNanogold labeling for TEM 2, 31 -- Thread-Topic: Reply on Post-embedding/sectioning FluoroNanogold labeling for 2, 31 -- TEM 2, 31 -- Thread-Index: AQHLvLQZqg//0Zu+HkardB4pWt5j2A== 2, 31 -- Message-ID: {F3EEF3767DFF3244826E72285C1C273448FA206741-at-EXCMSMBX03.ad.bcm.edu} 2, 31 -- Accept-Language: en-US 2, 31 -- Content-Language: en-US 2, 31 -- X-MS-Has-Attach: 2, 31 -- X-MS-TNEF-Correlator: 2, 31 -- acceptlanguage: en-US 2, 31 -- x-tm-as-product-ver: SMEX-8.0.0.1307-6.500.1024-17916.000 2, 31 -- x-tm-as-result: No--53.286300-8.000000-31 2, 31 -- x-tm-as-user-approved-sender: No 2, 31 -- x-tm-as-user-blocked-sender: No 2, 31 -- Content-Type: text/plain; charset="us-ascii" 2, 31 -- MIME-Version: 1.0 2, 31 -- Content-Transfer-Encoding: 8bit 2, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0PHK2ZE013593 ==============================End of - Headers==============================
The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new dual-beam scanning electron microscope, which support projects involving electron tomography, single particle analysis and electron crystallography of both stained and frozen-hydrated samples. In general, projects focus on 3D reconstruction of biological assemblies and examples range from the atomic structure of membrane proteins, to the subunit organization in macromolecular complexes and the cellular anatomy within the developing nematode. Implementation of new technologies is an ongoing interest at NYSBC and, with the dual-beam microscope, NYSBC plans to expand the scale of 3D reconstructions to encompass the characterization of entire cells and their distributions within their native tissue. To assist in these developments, NYSBC seeks a individual with postdoctoral experience in biological electron microscopy and image reconstruction. This individual will carry out experiments in support of collaborative projects with affiliated investigators, including EM sample preparation, data collection and image processing. Day-to-day duties also include assisting a wide range of users from the NYSBC¹s ten Member Institutions and setup and maintenance of microscopes. The individual should be capable of multitasking and should enjoy working with other people. Good communication skills are essential. Qualified applicants should send a curriculum vitae and names of three references to David Stokes (stokes-at-nysbc.org). Salary is commensurate with experience. The position is currently open and applications will be reviewed continuously until the position is filled.
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 5, 32 -- From Fengxia.Liang-at-med.nyu.edu Tue Jan 25 12:41:58 2011 5, 32 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PIfwWC031891 5, 32 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 12:41:58 -0600 5, 32 -- X-IronPort-Anti-Spam-Filtered: true 5, 32 -- X-IronPort-Anti-Spam-Result: ApsEAI+oPk0KkyXl/2dsb2JhbAClZbNmiGiFTwSFFw 5, 32 -- X-IronPort-AV: E=Sophos;i="4.60,375,1291611600"; 5, 32 -- d="scan'208";a="112814037" 5, 32 -- Received: from msgwsdcpht63.nyumc.org ([10.147.37.229]) 5, 32 -- by mail4.nyumc.org with SMTP; 25 Jan 2011 13:41:57 -0500 5, 32 -- Received: from MSGWSDCPMB05.nyumc.org ([10.147.54.52]) by 5, 32 -- MSGWSDCPHT63.nyumc.org ([10.147.37.229]) with mapi; Tue, 25 Jan 2011 13:41:57 5, 32 -- -0500 5, 32 -- From: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 5, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 32 -- Date: Tue, 25 Jan 2011 13:41:55 -0500 5, 32 -- Subject: Research Scientist Position in the Cryo-Electron Microscopy 5, 32 -- Facility at the New York Structural Biology Center 5, 32 -- Thread-Topic: Research Scientist Position in the Cryo-Electron Microscopy 5, 32 -- Facility at the New York Structural Biology Center 5, 32 -- Thread-Index: Acu8v4n8+leGz3MMmEyz3tESfhi+gg== 5, 32 -- Message-ID: {C96483A3.66F9%Fengxia.Liang-at-med.nyu.edu} 5, 32 -- Accept-Language: en-US 5, 32 -- Content-Language: en-US 5, 32 -- X-MS-Has-Attach: 5, 32 -- X-MS-TNEF-Correlator: 5, 32 -- user-agent: Microsoft-Entourage/13.4.0.100208 5, 32 -- acceptlanguage: en-US 5, 32 -- Content-Type: text/plain; charset="iso-8859-1" 5, 32 -- MIME-Version: 1.0 5, 32 -- Content-Transfer-Encoding: 8bit 5, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0PIfwWC031891 ==============================End of - Headers==============================
Microscopists, I am posting this on behalf of my colleague Mike Jercinovic. He is not a current subscriber so please be sure to cc him on any reply. His email is: mjj-at-geo.umass.edu. Thanks! TB
We have recently acquired an old Edwards Xenosput XE200 magnetron sputter coater. Does anyone have experience with these? It certainly produces an amazing coat, but has a rather bothersome problem. Often when we actuate the vacuum valve to start the initial pump down, or sometimes when we close the valve, the display goes haywire. The vacuum, head power, and head current readings all go crazy. The vacuum valve is pretty aggressive, so the whole thing gets a jolt when it opens or closes, so it seems like something might just be loose somewhere. This behavior can (sometimes) go away when switching the power off and on, or sometimes when cycling things a few times, but as this is happening quite frequently, the coater is almost unusable at this point. Today, it happened just when we vented it to put samples in. We got a "parts" machine along with the "working unit" (both obtained directly from Edwards), and I have now replaced the switching relay, the display electronics, the control electronics, and cables to the big board which has the terminal blocks....no change in behavior. I have reset all the physical interconnections I can find. Before launching in on further parts swapping, I thought it would be worth an enquiry to the community. Edwards no longer sells or supports this machine at all, so we are basically on our own.
Thanks for any help. Mike Jercinovic. UMass Geosciences.
==============================Original Headers============================== 3, 19 -- From baskin-at-bio.umass.edu Tue Jan 25 15:54:14 2011 3, 19 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 3, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0PLsEDI021802 3, 19 -- for {microscopy-at-microscopy.com} ; Tue, 25 Jan 2011 15:54:14 -0600 3, 19 -- Received: from [172.30.52.170] (neutopia.bio.umass.edu [128.119.55.8]) 3, 19 -- (authenticated bits=0) 3, 19 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id p0PLsBaq001782 3, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 3, 19 -- Tue, 25 Jan 2011 16:54:12 -0500 (EST) 3, 19 -- Mime-Version: 1.0 3, 19 -- Message-Id: {p06240506c964f5e26fed-at-[172.30.52.170]} 3, 19 -- Date: Tue, 25 Jan 2011 16:54:10 -0500 3, 19 -- To: microscopy-at-microscopy.com 3, 19 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 3, 19 -- Subject: Edwards Xenosput XE200 problem 3, 19 -- Cc: Mike Jercinovic {mjj-at-geo.umass.edu} 3, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 19 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Tue, 25 Jan 2011 16:54:12 -0500 (EST) 3, 19 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gul417-at-mail.usask.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Yes, the broken part is exactly as same as yours: at the bit on that connects to the head terminal. More frustrating, I can tell that it has broken at least 3 times from the record of usages--apparently a design flaw.
I might try to sold them together according to suggestions from the listers (I thank you all who replied to my email).
Best regards,
Guosheng Liu
----------------------- Hi there;
I was in the exact same boat as you, except I have an S150A coater. My HV cable broke (actually corroded and rotted away) right at the bit on that connects to the sputter head terminal. I called Edwards and spent a long time trying to even convince their service people that they made this piece of equipment. I ended up having to fax them copies of their own manual to show them the parts I was talking about.
Long story short: I never got anything from Edwards (useless). In the end I crimped to the end of the cable an eye-lug terminal and we just screw it onto the top of the sputter head now.
Best regards, Steven Cogswell, P.Eng. UNB Microscopy and Microanalysis
On Mon, Jan 24, 2011 at 8:15 PM, {gul417-at-mail.usask.ca} wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so } when replying } } please copy both gul417-at-mail.usask.ca as well as the } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: gul417-at-mail.usask.ca } } Name: Guosheng Liu } } } } Organization: University of Saskatchewan } } } } Title-Subject: [Filtered] Edwards S150B sputter coater part: } insulator/cable } } } } Message: Hello, } } } } The insulator cable in our aged Edwards S150B sputter coater broke } } (so no HT) a few days ago. Does anyone have a clue on where can I } } order this part (or a compatible part from other makes) or get a } } service (I'm in Canada)? } } } } Thanks in advance. } } } } Guosheng } }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Artur.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Artur.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Beam problem
Message: Hi I am an EPMA SX100 Operator and it has a unknown problem during 2 weeks. when i choose HV(Kv) it getting on but HV is always became 0 , I checked the everywhere and changed Filament, also there is no error. but how much I try and give Hv it never changed. please help me in this situation. Kazem
This does not look like a self fix task as it seems that you have a problem with your high voltage system itself that is why it will not switch on. The only other possible reason is that the safety device which prevents the HT switching on under a poor vacuum could be at fault?
One question does the emission/beam current meter alter at all when you switch on the HT? If it does it is not the safety device.
Steve
-----Original Message----- X-from: Artur.irani-at-yahoo.com [mailto:Artur.irani-at-yahoo.com] Sent: 26 January 2011 13:57 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Artur.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Artur.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Beam problem
Message: Hi I am an EPMA SX100 Operator and it has a unknown problem during 2 weeks. when i choose HV(Kv) it getting on but HV is always became 0 , I checked the everywhere and changed Filament, also there is no error. but how much I try and give Hv it never changed. please help me in this situation. Kazem
For those interested in cellular microscopy there is now The Cell: An Image Library - http://www.cellimagelibrary.org/
Also feel free to join The Cell LinkedIn Group - http://www.linkedin.com/groupRegistration?gid=3733425
David
David Orloff Manager, Image Library The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 T: 301-347-9300/Direct: 301-347-9305 F: 301-347-9310 E-mail: dorloff-at-ascb.org Web site: www.ascb.org The Cell: An Image LibraryT: www.cellimagelibrary.org LinkedIn Group: http://www.linkedin.com/groupRegistration?gid=3733425
==============================Original Headers============================== 8, 23 -- From DOrloff-at-ascb.org Wed Jan 26 09:12:03 2011 8, 23 -- Received: from smtp.ascb.org (jscpp.org [173.13.233.102] (may be forged)) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0QFC3I3017865 8, 23 -- for {Microscopy-at-Microscopy.Com} ; Wed, 26 Jan 2011 09:12:03 -0600 8, 23 -- Received: from ASCB2.ascb.local ([::1]) by ASCB2.ascb.local ([::1]) with mapi; 8, 23 -- Wed, 26 Jan 2011 10:12:00 -0500 8, 23 -- From: David Orloff {DOrloff-at-ascb.org} 8, 23 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 8, 23 -- CC: David Orloff {DOrloff-at-ascb.org} 8, 23 -- Date: Wed, 26 Jan 2011 10:11:59 -0500 8, 23 -- Subject: The Cell: An Image Library - LinkedIn group 8, 23 -- Thread-Topic: The Cell: An Image Library - LinkedIn group 8, 23 -- Thread-Index: Acu9a2D302NV3t/tQGqajHGfrnk6xQ== 8, 23 -- Message-ID: {8D9C2A7731877D45B1691E78451EC3BE4C6BE127-at-ASCB2.ascb.local} 8, 23 -- Accept-Language: en-US 8, 23 -- Content-Language: en-US 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- acceptlanguage: en-US 8, 23 -- Content-Type: text/plain; charset="iso-8859-1" 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0QFC3I3017865 ==============================End of - Headers==============================
Hello, We have another problem with our Philips CM100. Yesterday afternoon, the operating system of the microscope crashed with strange OPCON screen (see the link bellow for image). No beeps, no buttons were working with exception of the "OFF button". After restart, CM100 started in “Default RAM init†mode.
Please, did anybody observed something similar? In our case it was for the first time. Any responses are welcome.
Image of OPCON screen after crash: http://www2.biomed.cas.cz/~benada/CM100_trouble.html
Best regards Oldrich
-------------------------- Oldrich Benada Institute of Microbiology, Acad. Sci. CR Videnska 1083 142 20 Prague 4 Czech Republic
==============================Original Headers============================== 6, 21 -- From benada-at-biomed.cas.cz Thu Jan 27 04:37:17 2011 6, 21 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0RAbGmx020693 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Jan 2011 04:37:17 -0600 6, 21 -- Received: from u117ob.localnet (a100ix.mbu.cas.cz [147.231.44.171]) 6, 21 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 21 -- (No client certificate requested) 6, 21 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id C851C1A44726 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 27 Jan 2011 11:37:15 +0100 (CET) 6, 21 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 6, 21 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 6, 21 -- To: microscopy-at-microscopy.com 6, 21 -- Subject: CM100 trouble 6, 21 -- Date: Thu, 27 Jan 2011 11:37:09 +0100 6, 21 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Type: text/plain; 6, 21 -- charset="utf-8" 6, 21 -- Message-Id: {201101271137.09396.benada-at-biomed.cas.cz} 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0RAbGmx020693 ==============================End of - Headers==============================
How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?
I would appreciate any experience or recommendations, also directly towards me.
Henning Stahlberg, PhD. Center for Cellular Imaging and Nano Analytics (C-CINA) Prof. for Structural Biology Biozentrum, University Basel at the Department of Biosystems Science and Engineering (D-BSSE) Mattenstrasse 26, WRO-1058 CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 (office) Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) Fax: +41-61-387 39 86 mailto:Henning.Stahlberg-at-unibas.ch Skype:henningstahlberg http://c-cina.org http://2dx.org
Postal Address for delivery of Letters / Goods / Express Carriers: C-CINA, Biozentrum University of Basel c/o Syngenta AG, WRO-1058.6.60, D-BSSE Schwarzwaldallee 215 Postfach CH-4002 Basel ___________________________________________________
==============================Original Headers============================== 10, 23 -- From hstahlberg-at-me.com Fri Jan 28 04:36:31 2011 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com [17.148.16.95]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SAaVKt022583 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 10, 23 -- MIME-version: 1.0 10, 23 -- Content-transfer-encoding: 7BIT 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange Server 7u4-20.01 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id {0LFQ009UNARUIS90-at-asmtp020.mac.com} 10, 23 -- for Microscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 (PST) 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 signatures=0 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 classifier=spam 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 definitions=main-1101280028 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- X-Mailer: Apple Mail (2.1082) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wxufocus-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wxufocus-at-gmail.com Name: Jizhong
Title-Subject: [Filtered] LM: Navitar Tube Lens for use with Infinity Corrected Objective
Message: Hi, I'm setting up an optical system with an infinity corrected objective lens in a special tool I'm designing. I'm thinking of using an adapter tube from Navitar to form the image for a CCD camera. The Adapter Tube has a nice C-mount for attaching the CCD camera. My question is: is the tube lens (to convert the parallel beams into a real image) resides inside the Adapter Tube? Or I need the Ultra Body Tube? Please see the sys diagram: http://machinevision.navitar.com/pdfs/Precise_Eye_System_Diagram.pdf
I am no longer the Director of Light Microscopy at St. Jude Children's Research Hospital. For questions regarding the use of the Cell and Tissue Imaging Center, please direct your inquiries to the team of Imaging Scientists in the CTIC, Jennifer Peters, Yannan Ouyang, and Jamshid Temirov. They may be reached as a group at the following email address: lightmicroscopy-at-stjude.org. The phone number in the CTIC is 901-595-3439.
Although no longer serving in my former capacity as the Director of Light Microscopy, I am currently available as a technical consultant to the Cell and Tissue Imaging Center. I may be reached directly as necessary at the following email address: samuel.connell-at-gmail.com
I am currently out of the office on extended leave. For assistance, please contact Mary Arnold (Mary.Arnold-at-MyFWC.com) and she will direct your inquiry to the appropriate staff member.
Earnest Truby
==============================Original Headers============================== 4, 24 -- From MAILER-DAEMON-at-ns.microscopy.com Fri Jan 28 07:02:10 2011 4, 24 -- Received: from FWC-TLEX8.fwc.state.fl.us (fwc-tlex8.fwc.state.fl.us [216.76.92.213]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SD2A6J031715 4, 24 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:02:10 -0600 4, 24 -- Received: from FWC-TLEX10.fwc.state.fl.us ([10.10.10.11]) by 4, 24 -- FWC-TLEX8.fwc.state.fl.us ([::1]) with mapi; Fri, 28 Jan 2011 08:02:09 -0500 4, 24 -- From: "Truby, Earnest" {Earnest.Truby-at-MyFWC.com} 4, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 4, 24 -- Date: Fri, 28 Jan 2011 08:02:09 -0500 4, 24 -- Subject: Out of Office: [Microscopy] viaWWW:LM: Navitar Tube Lens for use 4, 24 -- with Infinity Corrected Objective 4, 24 -- Thread-Topic: [Microscopy] viaWWW:LM: Navitar Tube Lens for use with 4, 24 -- Infinity Corrected Objective 4, 24 -- Thread-Index: Acu+65JgCw5Sxg5/S5m5kJr436pjrQAAAAIN 4, 24 -- Message-ID: {EA8D27879805554FA1182CA24E21659B063437097A-at-FWC-TLEX10.fwc.state.fl.us} 4, 24 -- Accept-Language: en-US 4, 24 -- Content-Language: en-US 4, 24 -- X-MS-Has-Attach: 4, 24 -- X-MS-TNEF-Correlator: 4, 24 -- acceptlanguage: en-US 4, 24 -- Content-Type: text/plain; charset="utf-8" 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Transfer-Encoding: 8bit 4, 24 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0SD2A6J031715 ==============================End of - Headers==============================
Thank you for your message. Please note that I will be out of the office for the rest of today, Thursday, January 27th. During this time I will have no access to voice mail or e-mail. I will respond to all messages accordingly upon my return to the office tomorrow Friday, January 28th.
If your matter is urgent and requires IMMEDIATE attention, please contact our main employment line at 301-846-5362 and the HR Assistant will be happy to connect you with the appropriate recruiter.
Please note that on occasion, the opening of Ft. Detrick may be delayed or closed due to inclement weather. For any weather related concerns and additional information about delays/closings, you may contact the Fort Detrick Weather Alert Hotline at 301-619-7611.
Thank you for your understanding. Make it a great day!
Regards, Nelmarie Miranda, PHR Senior Employment Specialist SAIC-Frederick, Inc.
==============================Original Headers============================== 7, 29 -- From MAILER-DAEMON-at-ns.microscopy.com Fri Jan 28 07:03:29 2011 7, 29 -- Received: from nihxway2out.hub.nih.gov (nihxway2out.hub.nih.gov [128.231.90.110]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SD3Tcr004075 7, 29 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:03:29 -0600 7, 29 -- X-IronPortListener: Outbound_SMTP 7, 29 -- X-IronPort-Anti-Spam-Filtered: true 7, 29 -- X-IronPort-Anti-Spam-Result: Av0EABZNQk2cKEcU/2dsb2JhbACEFKAAbHOjAYgrLJBEgSOBboFKdASFGIpu 7, 29 -- Received: from unknown (HELO NIHHT01.nih.gov) ([156.40.71.20]) 7, 29 -- by nihxway2out.hub.nih.gov with ESMTP; 28 Jan 2011 08:03:28 -0500 7, 29 -- Received: from NIHMLBX01.nih.gov ([156.40.71.31]) by NIHHT01.nih.gov 7, 29 -- ([156.40.71.20]) with mapi; Fri, 28 Jan 2011 08:03:29 -0500 7, 29 -- From: "Miranda, Nelmarie (NIH/NCI) [C]" {mirandan-at-mail.nih.gov} 7, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 29 -- Date: Fri, 28 Jan 2011 08:03:28 -0500 7, 29 -- Subject: Out of Office: [Microscopy] viaWWW:LM: Navitar Tube Lens for use 7, 29 -- with Infinity Corrected Objective 7, 29 -- Thread-Topic: [Microscopy] viaWWW:LM: Navitar Tube Lens for use with 7, 29 -- Infinity Corrected Objective 7, 29 -- Thread-Index: Acu+68GC0NnXIpGKSnS8gOhK8ij6BAAAAAGv 7, 29 -- Message-ID: {3D7FD38AAD34A84982D156F22703B0E0028557615C-at-NIHMLBX01.nih.gov} 7, 29 -- Accept-Language: en-US 7, 29 -- Content-Language: en-US 7, 29 -- X-MS-Has-Attach: 7, 29 -- X-MS-TNEF-Correlator: 7, 29 -- acceptlanguage: en-US 7, 29 -- Content-Type: text/plain; charset="utf-8" 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p0SD3Tcr004075 ==============================End of - Headers==============================
I will be out of the office from 1/20/11 - 1/28/11, and will have only limited access to my email. I will respond to your email when I am back in the office. In urgent cases, please contact Jason Wickersham at Jason.Wickersham-at-resaltatech.com, or call (551)-804-1845.
Thank you for your understanding.
Mike Bode ResAlta Research Technologies
==============================Original Headers============================== 1, 27 -- From resalta1-at-web38.justhost.com Fri Jan 28 07:06:25 2011 1, 27 -- Received: from web38.justhost.com (web38.justhost.com [99.198.119.34]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SD6PI3015614 1, 27 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:06:25 -0600 1, 27 -- Received: from resalta1 by web38.justhost.com with local (Exim 4.69) 1, 27 -- (envelope-from {resalta1-at-web38.justhost.com} ) 1, 27 -- id 1Pio1x-0002N7-Tw 1, 27 -- for microscopy-at-microscopy.com; Fri, 28 Jan 2011 07:06:25 -0600 1, 27 -- To: microscopy-at-microscopy.com 1, 27 -- X-Autorespond: [Microscopy] viaWWW:LM: Navitar Tube Lens for use with Infinity Corrected Objective 1, 27 -- MIME-Version: 1.0 1, 27 -- X-Loop: microscopy-at-microscopy.com 1, 27 -- Precedence: auto_reply 1, 27 -- X-Precedence: auto_reply 1, 27 -- From: "mike.bode-at-resaltatech.com" {mike.bode-at-resaltatech.com} 1, 27 -- Content-type: text/plain; charset=ansi_x3.110-1983 1, 27 -- Subject: Mike Bode is out of the office. Re: [Microscopy] viaWWW:LM: Navitar Tube Lens for use with Infinity Corrected Objective 1, 27 -- Message-Id: {E1Pio1x-0002N7-Tw-at-web38.justhost.com} 1, 27 -- Date: Fri, 28 Jan 2011 07:06:25 -0600 1, 27 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 1, 27 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 1, 27 -- X-AntiAbuse: Original Domain - microscopy.com 1, 27 -- X-AntiAbuse: Originator/Caller UID/GID - [505 32003] / [47 12] 1, 27 -- X-AntiAbuse: Sender Address Domain - web38.justhost.com 1, 27 -- X-Source: /usr/local/cpanel/bin/autorespond 1, 27 -- X-Source-Args: /usr/local/cpanel/bin/autorespond mike.bode-at-resaltatech.com /home/resalta1/.autorespond 1, 27 -- X-Source-Dir: / ==============================End of - Headers==============================
Hello, Many thanks for all advices and suggestions. The most likely it was a power spike that caused the problem. Now, our CM100 is working again. Unfortunately, we have only DOS remote control package for microscope setting backup and our old DOS PC does not boot any more. It took me half a day to align and recalibrate the microscope manually after its RAM init. I'm thinking about setup a virtual box with DOS and remote control package in near future.
I merged together all the replies and they are available on following link: http://www2.biomed.cas.cz/~benada/CM100_trouble_r.html
Many thanks again Oldrich
} } Hello, } } We have another problem with our Philips CM100. Yesterday afternoon, the } } operating system of the microscope crashed with strange OPCON screen } } (see the link bellow for image). No beeps, no buttons were working with } } exception of the "OFF button". After restart, CM100 started in “Default } } RAM init” mode. } } } } Please, did anybody observed something similar? In our case it was for } } the first time. Any responses are welcome. } } } } Image of OPCON screen after crash: } } http://www2.biomed.cas.cz/~benada/CM100_trouble.html } } } } Best regards } } } } Oldrich } } } } -------------------------- } } Oldrich Benada } } Institute of Microbiology, Acad. Sci. CR } } Videnska 1083 } } 142 20 Prague 4 } } Czech Republic
==============================Original Headers============================== 5, 23 -- From benada-at-biomed.cas.cz Fri Jan 28 07:27:17 2011 5, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SDRHtI015581 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:27:17 -0600 5, 23 -- Received: from u117ob.localnet (u117ob.mbu.cas.cz [147.231.44.101]) 5, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 23 -- (No client certificate requested) 5, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 6439D1A44664 5, 23 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:27:16 +0100 (CET) 5, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 5, 23 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 5, 23 -- To: microscopy-at-microscopy.com 5, 23 -- Subject: Re: [Microscopy] CM100 trouble - solved (hopefully) 5, 23 -- Date: Fri, 28 Jan 2011 14:27:08 +0100 5, 23 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 5, 23 -- References: {201101271037.p0RAbUuW020819-at-ns.microscopy.com} {C0F02D25-2F23-4835-9929-5C16610E5E47-at-rochester.rr.com} 5, 23 -- In-Reply-To: {C0F02D25-2F23-4835-9929-5C16610E5E47-at-rochester.rr.com} 5, 23 -- MIME-Version: 1.0 5, 23 -- Content-Type: Text/Plain; 5, 23 -- charset="windows-1252" 5, 23 -- Message-Id: {201101281427.09048.benada-at-biomed.cas.cz} 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0SDRHtI015581 ==============================End of - Headers==============================
I'm not sure of the distance, but you should be able to use a cheap gaussmeter to measure the fields from the NMR. Even a DC field can cause problems for a TEM, particularly a high-end FEG TEM. Anyone who has worked with magnetic samples can attest to the problems they cause.
While there may be strong DC fields, NMRs work by flipping nuclear magnetic moments which suggests there are AC or pulsed components to the field.
You do not want this beast close to your TEM column.
Cheers, Henk
At 1/28/2011 5:37 AM, hstahlberg-at-me.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hi, } } How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ? } } I would appreciate any experience or recommendations, also directly towards me. } } Thanks, } } Henning. } } ___________________________________________________ } } Henning Stahlberg, PhD. } Center for Cellular Imaging and Nano Analytics (C-CINA) } Prof. for Structural Biology } Biozentrum, University Basel } at the Department of Biosystems Science and Engineering (D-BSSE) } Mattenstrasse 26, WRO-1058 } CH-4058 Basel, Switzerland } Tel: +41-61-387 32 62 (office) } Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) } Fax: +41-61-387 39 86 } mailto:Henning.Stahlberg-at-unibas.ch } Skype:henningstahlberg } http://c-cina.org } http://2dx.org } } Postal Address for delivery of Letters / Goods / Express Carriers: } C-CINA, Biozentrum University of Basel } c/o Syngenta AG, WRO-1058.6.60, D-BSSE } Schwarzwaldallee 215 } Postfach } CH-4002 Basel } ___________________________________________________ } } ==============================Original Headers============================== } 10, 23 -- Fromhstahlberg-at-me.com Fri Jan 28 04:36:31 2011 } 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com [17.148.16.95]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SAaVKt022583 } 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 } 10, 23 -- MIME-version: 1.0 } 10, 23 -- Content-transfer-encoding: 7BIT } 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII } 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) } 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange Server 7u4-20.01 } 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id {0LFQ009UNARUIS90-at-asmtp020.mac.com} } 10, 23 -- forMicroscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 (PST) } 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure } 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 } 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 signatures=0 } 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 } 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 classifier=spam } 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 definitions=main-1101280028 } 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} } 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM } 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 } 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} } 10, 23 -- To:Microscopy-at-microscopy.com } 10, 23 -- X-Mailer: Apple Mail (2.1082) } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 8, 26 -- From colijn.1-at-osu.edu Fri Jan 28 07:46:42 2011 8, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SDkgWe015146 8, 26 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 07:46:42 -0600 8, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 8, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 26 -- id {01NX5G33RU409EGJJ4-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 8, 26 -- Fri, 28 Jan 2011 08:46:42 -0500 (EST) 8, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 8, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 26 -- with ESMTPA id {01NX5G33FUQS9EIROA-at-ecr6.ohio-state.edu} for 8, 26 -- microscopy-at-microscopy.com; Fri, 28 Jan 2011 08:46:42 -0500 (EST) 8, 26 -- Date: Fri, 28 Jan 2011 08:46:41 -0500 8, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 8, 26 -- Subject: Re: [Microscopy] How close can a NMR machine be installed next to a TEM 8, 26 -- In-reply-to: {201101281037.p0SAbvsr024540-at-ns.microscopy.com} 8, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 8, 26 -- To: microscopy-at-microscopy.com 8, 26 -- Message-id: {4D42C8C1.3090601-at-osu.edu} 8, 26 -- MIME-version: 1.0 8, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 26 -- Content-transfer-encoding: 7bit 8, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) 8, 26 -- Gecko/20101207 Thunderbird/3.1.7 8, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 8, 26 -- References: {201101281037.p0SAbvsr024540-at-ns.microscopy.com} ==============================End of - Headers==============================
We have a Philips CM10 TEM. Jeol checked for problems prior to installing a Jeol Eclipse 300MHz superconducting magnet instrument 13m and two rooms away. As far as we know there was no interference.
Dave
-----Original Message----- X-from: hstahlberg-at-me.com [mailto:hstahlberg-at-me.com] Sent: 28 January 2011 10:40 To: David Patton
Hi,
How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?
I would appreciate any experience or recommendations, also directly towards me.
Henning Stahlberg, PhD. Center for Cellular Imaging and Nano Analytics (C-CINA) Prof. for Structural Biology Biozentrum, University Basel at the Department of Biosystems Science and Engineering (D-BSSE) Mattenstrasse 26, WRO-1058 CH-4058 Basel, Switzerland Tel: +41-61-387 32 62 (office) Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) Fax: +41-61-387 39 86 mailto:Henning.Stahlberg-at-unibas.ch Skype:henningstahlberg http://c-cina.org http://2dx.org
Postal Address for delivery of Letters / Goods / Express Carriers: C-CINA, Biozentrum University of Basel c/o Syngenta AG, WRO-1058.6.60, D-BSSE Schwarzwaldallee 215 Postfach CH-4002 Basel ___________________________________________________
==============================Original Headers============================== 10, 23 -- From hstahlberg-at-me.com Fri Jan 28 04:36:31 2011 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com [17.148.16.95]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SAaVKt022583 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 10, 23 -- MIME-version: 1.0 10, 23 -- Content-transfer-encoding: 7BIT 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange Server 7u4-20.01 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id {0LFQ009UNARUIS90-at-asmtp020.mac.com} 10, 23 -- for Microscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 (PST) 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 signatures=0 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 classifier=spam 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 definitions=main-1101280028 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} 10, 23 -- To: Microscopy-at-microscopy.com 10, 23 -- X-Mailer: Apple Mail (2.1082) ==============================End of - Headers==============================
==============================Original Headers============================== 20, 49 -- From David.Patton-at-uwe.ac.uk Fri Jan 28 09:26:18 2011 20, 49 -- Received: from DB3EHSOBE005.bigfish.com (db3ehsobe005.messaging.microsoft.com [213.199.154.143]) 20, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SFQH8A007281 20, 49 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 09:26:17 -0600 20, 49 -- Received: from mail57-db3-R.bigfish.com (10.3.81.247) by 20, 49 -- DB3EHSOBE005.bigfish.com (10.3.84.25) with Microsoft SMTP Server id 20, 49 -- 14.1.225.8; Fri, 28 Jan 2011 15:26:16 +0000 20, 49 -- Received: from mail57-db3 (localhost.localdomain [127.0.0.1]) by 20, 49 -- mail57-db3-R.bigfish.com (Postfix) with ESMTP id 741F8AD8278; Fri, 28 Jan 20, 49 -- 2011 15:26:16 +0000 (UTC) 20, 49 -- X-SpamScore: -25 20, 49 -- X-BigFish: VPS-25(zzbb2cK936eK7f0R542N4015Lzz1202hz31iz8275bh8275dhz2fh668h) 20, 49 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:mailapp04.uwe.ac.uk;RD:mailapp04.uwe.ac.uk;EFVD:NLI 20, 49 -- Received: from mail57-db3 (localhost.localdomain [127.0.0.1]) by mail57-db3 20, 49 -- (MessageSwitch) id 1296228376153955_25127; Fri, 28 Jan 2011 15:26:16 +0000 20, 49 -- (UTC) 20, 49 -- Received: from DB3EHSMHS011.bigfish.com (unknown [10.3.81.254]) by 20, 49 -- mail57-db3.bigfish.com (Postfix) with ESMTP id 215BF128804C; Fri, 28 Jan 2011 20, 49 -- 15:26:16 +0000 (UTC) 20, 49 -- Received: from mailapp04.uwe.ac.uk (164.11.132.66) by DB3EHSMHS011.bigfish.com 20, 49 -- (10.3.87.111) with Microsoft SMTP Server id 14.1.225.8; Fri, 28 Jan 2011 20, 49 -- 15:26:12 +0000 20, 49 -- Received: from (egen-hub01.uwe.ac.uk [164.11.251.45]) by mailapp04.uwe.ac.uk 20, 49 -- with smtp id 784e_1182_f038fb40_2af2_11e0_b166_00142223915c; Fri, 28 Jan 20, 49 -- 2011 15:26:12 +0000 20, 49 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by 20, 49 -- egen-hub01.campus.ads.uwe.ac.uk ([164.11.251.45]) with mapi; Fri, 28 Jan 2011 20, 49 -- 15:24:56 +0000 20, 49 -- From: David Patton {David.Patton-at-uwe.ac.uk} 20, 49 -- To: "'hstahlberg-at-me.com'" {hstahlberg-at-me.com} 20, 49 -- Date: Fri, 28 Jan 2011 15:24:57 +0000 20, 49 -- Subject: RE: [Microscopy] How close can a NMR machine be installed next to a 20, 49 -- TEM 20, 49 -- Thread-Topic: [Microscopy] How close can a NMR machine be installed next to 20, 49 -- a TEM 20, 49 -- Thread-Index: Acu+17j4uzXKSG+KT0+0K5ae79FPDAAJ0GMA 20, 49 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF433F9B96DCB-at-EGEN-MBX02.campus.ads.uwe.ac.uk} 20, 49 -- References: {201101281040.p0SAe1l6027719-at-ns.microscopy.com} 20, 49 -- In-Reply-To: {201101281040.p0SAe1l6027719-at-ns.microscopy.com} 20, 49 -- Accept-Language: en-US, en-GB 20, 49 -- Content-Language: en-US 20, 49 -- X-MS-Has-Attach: 20, 49 -- X-MS-TNEF-Correlator: 20, 49 -- acceptlanguage: en-US, en-GB 20, 49 -- Content-Type: text/plain; charset="us-ascii" 20, 49 -- MIME-Version: 1.0 20, 49 -- X-OriginatorOrg: uwe.ac.uk 20, 49 -- Content-Transfer-Encoding: 8bit 20, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0SFQH8A007281 ==============================End of - Headers==============================
Part of our microscopy lab is next door to the NMR lab. The high electro-magnetic fields (presumably from the high voltage lines feeding the NMR) preclude installation of SEM or TEM in areas near the adjoining wall. A distance of only a few meters is sufficient for the field strength to drop to safe levels.
We have a similar problem with the HRTEM (located ~30 meters from the NMR lab) in that a major high voltage conduit is located in the ceiling. As the EMF will drop with the square of the distance, the ~2 meter separation from conduit to TEM column is (just) sufficient to permit unimpeded operation.
The main point to take away from this is that any space MUST be checked for fields and vibrations before installing any microscope. This should be required by the TEM maker. There may be sources of EMF that are not obvious.
Cheers Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
} From: {hstahlberg-at-me.com} } Reply-To: {hstahlberg-at-me.com} } Date: Fri, 28 Jan 2011 04:40:50 -0600 } To: {raristau-at-ims.uconn.edu} } Subject: [Microscopy] How close can a NMR machine be installed next to a TEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } Hi, } } How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year } old NMR machine? If both are on the same floor, then the magnetic field lines } from the NMR should run through the TEM column vertically, so that the } sensitivity for the strong DC fields of the NMR machine might be less ? } } I would appreciate any experience or recommendations, also directly towards } me. } } Thanks, } } Henning. } } ___________________________________________________ } } Henning Stahlberg, PhD. } Center for Cellular Imaging and Nano Analytics (C-CINA) } Prof. for Structural Biology } Biozentrum, University Basel } at the Department of Biosystems Science and Engineering (D-BSSE) } Mattenstrasse 26, WRO-1058 } CH-4058 Basel, Switzerland } Tel: +41-61-387 32 62 (office) } Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant) } Fax: +41-61-387 39 86 } mailto:Henning.Stahlberg-at-unibas.ch } Skype:henningstahlberg } http://c-cina.org } http://2dx.org } } Postal Address for delivery of Letters / Goods / Express Carriers: } C-CINA, Biozentrum University of Basel } c/o Syngenta AG, WRO-1058.6.60, D-BSSE } Schwarzwaldallee 215 } Postfach } CH-4002 Basel } ___________________________________________________ } } ==============================Original Headers============================== } 10, 23 -- From hstahlberg-at-me.com Fri Jan 28 04:36:31 2011 } 10, 23 -- Received: from asmtpout020.mac.com (asmtpout020.mac.com } [17.148.16.95]) } 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p0SAaVKt022583 } 10, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 04:36:31 -0600 } 10, 23 -- MIME-version: 1.0 } 10, 23 -- Content-transfer-encoding: 7BIT } 10, 23 -- Content-type: text/plain; CHARSET=US-ASCII } 10, 23 -- Received: from [192.168.1.35] ([92.104.21.174]) } 10, 23 -- by asmtp020.mac.com (Oracle Communications Messaging Exchange } Server 7u4-20.01 } 10, 23 -- 64bit (built Nov 21 2010)) with ESMTPSA id } {0LFQ009UNARUIS90-at-asmtp020.mac.com} } 10, 23 -- for Microscopy-at-microscopy.com; Fri, 28 Jan 2011 02:36:31 -0800 } (PST) } 10, 23 -- X-Proofpoint-Virus-Version: vendor=fsecure } 10, 23 -- engine=2.50.10432:5.2.15,1.0.148,0.0.0000 } 10, 23 -- definitions=2011-01-28_04:2011-01-28,2011-01-28,1970-01-01 } signatures=0 } 10, 23 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 } spamscore=0 } 10, 23 -- ipscore=0 suspectscore=1 phishscore=0 bulkscore=0 adultscore=0 } classifier=spam } 10, 23 -- adjust=0 reason=mlx engine=6.0.2-1012030000 } definitions=main-1101280028 } 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com} } 10, 23 -- Subject: How close can a NMR machine be installed next to a TEM } 10, 23 -- Date: Fri, 28 Jan 2011 11:35:53 +0100 } 10, 23 -- Message-id: {A81EC18B-D20C-4687-9FEA-E77A009400F7-at-me.com} } 10, 23 -- To: Microscopy-at-microscopy.com } 10, 23 -- X-Mailer: Apple Mail (2.1082) } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 25 -- From raristau-at-ims.uconn.edu Fri Jan 28 11:02:54 2011 8, 25 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SH2rNh026964 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 11:02:53 -0600 8, 25 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu [137.99.20.234]) 8, 25 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id A66B92DF9A; 8, 25 -- Fri, 28 Jan 2011 12:02:52 -0500 (EST) 8, 25 -- User-Agent: Microsoft-Entourage/11.4.0.080122 8, 25 -- Date: Fri, 28 Jan 2011 12:02:50 -0500 8, 25 -- Subject: Re: [Microscopy] How close can a NMR machine be installed next to 8, 25 -- a TEM 8, 25 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 8, 25 -- To: {hstahlberg-at-me.com} , 8, 25 -- "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 8, 25 -- Message-ID: {C96860EA.22E5%raristau-at-ims.uconn.edu} 8, 25 -- Thread-Topic: [Microscopy] How close can a NMR machine be installed next 8, 25 -- to a TEM 8, 25 -- Thread-Index: Acu/DTG6cBV6NCsAEeC6lgAbY55CVA== 8, 25 -- In-Reply-To: {201101281040.p0SAeoxM028470-at-ns.microscopy.com} 8, 25 -- Mime-version: 1.0 8, 25 -- Content-type: text/plain; 8, 25 -- charset="US-ASCII" 8, 25 -- Content-transfer-encoding: 7bit 8, 25 -- X-Virus-Scanned: clamav-milter 0.96.1 at mta1 8, 25 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I would be interested in a summary of the answers received on this. We have a new instrument that may be installed within 200feet of an older model Varian MRI system, it is a superconductor, and the magnet is 4.7T. I do not believe that it is shielded the way the more modern MRIs are so I am curious what the field is out at 200ft from the instrument. I have the expressions for the fields from a solenoid at a distance, but I would rather have measurements than guesstimates, particularly when the kinds of static fields that we wish to avoid are probably in the milligauss or better regime. I will probably find an instrument (MRI) is operational and plot the fields as a function of both distance and angle to the principal axis of the MRI.
-- John Mansfield PhD Cphys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 10, 20 -- From jfmjfm-at-umich.edu Fri Jan 28 12:25:29 2011 10, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SIPSj3028806 10, 20 -- for {microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:25:28 -0600 10, 20 -- Received: FROM [141.212.214.158] (Unknown [141.212.214.158]) 10, 20 -- By hellskitchen.mr.itd.umich.edu ID 4D430A15.63C08.15230 ; 10, 20 -- Authuser jfmjfm; 10, 20 -- 28 Jan 2011 13:25:25 EST 10, 20 -- Subject: Re: [Microscopy] RE: How close can a NMR machine be installed next to a 10, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) 10, 20 -- Content-Type: text/plain; charset=iso-8859-1 10, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 10, 20 -- In-Reply-To: {201101281536.p0SFa58Y021495-at-ns.microscopy.com} 10, 20 -- Date: Fri, 28 Jan 2011 13:25:23 -0500 10, 20 -- Message-Id: {8999101F-6C91-4A0D-B53D-645FF0B7700A-at-umich.edu} 10, 20 -- References: {201101281536.p0SFa58Y021495-at-ns.microscopy.com} 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- X-Mailer: Apple Mail (2.1082) 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0SIPSj3028806 ==============================End of - Headers==============================
I have a hard time getting the triglycerides to dye in 1 micron green algae due to their thick cell wall. Any suggestions? I plan to view this on a confocal laser scanning microscope.
Thanks!
==============================Original Headers============================== 5, 21 -- From dmywong-at-ucdavis.edu Fri Jan 28 14:36:09 2011 5, 21 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SKa7qA016884 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:36:09 -0600 5, 21 -- Received: by yxl31 with SMTP id 31so1504926yxl.0 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:36:06 -0800 (PST) 5, 21 -- Received: by 10.91.196.17 with SMTP id y17mr5625040agp.207.1296246965726; 5, 21 -- Fri, 28 Jan 2011 12:36:05 -0800 (PST) 5, 21 -- Received: from [192.168.1.101] ([128.120.242.213]) 5, 21 -- by mx.google.com with ESMTPS id w4sm22209508anw.36.2011.01.28.12.36.04 5, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 5, 21 -- Fri, 28 Jan 2011 12:36:04 -0800 (PST) 5, 21 -- Message-Id: {012F709C-C0D0-42BE-8157-89CF50915E61-at-ucdavis.edu} 5, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 5, 21 -- To: Microscopy-at-microscopy.com 5, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 5, 21 -- Subject: CLSM: Dying Triglyceride of microalgae 5, 21 -- Date: Fri, 28 Jan 2011 12:36:02 -0800 5, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
I am trying to do quantification of my cells by measuring the diameter of the fluorescence from a 3D construction image. I have heard of ImageJ, but I am not sure what pluggins to install. Have you tried a better program? ______________________________ Diana M. Wong Graduate Student
Franz Group University of California, Davis Department of Chemistry One Shields Ave Davis, CA 95616 dmywong-at-ucdavis.edu http://chemgroups.ucdavis.edu/~franz/
==============================Original Headers============================== 6, 21 -- From dmywong-at-ucdavis.edu Fri Jan 28 14:41:43 2011 6, 21 -- Received: from mail-gw0-f41.google.com (mail-gw0-f41.google.com [74.125.83.41]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SKfhX7020432 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:41:43 -0600 6, 21 -- Received: by gwj22 with SMTP id 22so1541232gwj.0 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:41:42 -0800 (PST) 6, 21 -- Received: by 10.90.84.7 with SMTP id h7mr5396488agb.55.1296247301077; 6, 21 -- Fri, 28 Jan 2011 12:41:41 -0800 (PST) 6, 21 -- Received: from [192.168.1.101] ([128.120.242.213]) 6, 21 -- by mx.google.com with ESMTPS id g2sm2838313yhc.28.2011.01.28.12.41.39 6, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 21 -- Fri, 28 Jan 2011 12:41:40 -0800 (PST) 6, 21 -- Message-Id: {D290C485-7F5E-45F1-B542-F8FEFBA0E7EC-at-ucdavis.edu} 6, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Confocal LSM: Quantification software? ImageJ 6, 21 -- Date: Fri, 28 Jan 2011 12:41:37 -0800 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Rather than going with the a la carte method of installing plugins for ImageJ* you could try one of the prepackaged collections. MBF ImageJ is a good prepackaged selection of plugins (http://www.macbiophotonics.ca/imagej/) You could also try Fiji (http://pacific.mpi-cbg.de/wiki/index.php/Fiji).
Regards,
Mike
(*Comments made above represent personal opinion and do not represent formal endorsement of any particular product, company, or organization by NIST.)
-----Original Message----- X-from: dmywong-at-ucdavis.edu [mailto:dmywong-at-ucdavis.edu] Sent: Friday, January 28, 2011 3:58 PM To: Anderson, Michael
I am trying to do quantification of my cells by measuring the diameter of the fluorescence from a 3D construction image. I have heard of ImageJ, but I am not sure what pluggins to install. Have you tried a better program? ______________________________ Diana M. Wong Graduate Student
Franz Group University of California, Davis Department of Chemistry One Shields Ave Davis, CA 95616 dmywong-at-ucdavis.edu http://chemgroups.ucdavis.edu/~franz/
==============================Original Headers============================== 6, 21 -- From dmywong-at-ucdavis.edu Fri Jan 28 14:41:43 2011 6, 21 -- Received: from mail-gw0-f41.google.com (mail-gw0-f41.google.com [74.125.83.41]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SKfhX7020432 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 14:41:43 -0600 6, 21 -- Received: by gwj22 with SMTP id 22so1541232gwj.0 6, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 12:41:42 -0800 (PST) 6, 21 -- Received: by 10.90.84.7 with SMTP id h7mr5396488agb.55.1296247301077; 6, 21 -- Fri, 28 Jan 2011 12:41:41 -0800 (PST) 6, 21 -- Received: from [192.168.1.101] ([128.120.242.213]) 6, 21 -- by mx.google.com with ESMTPS id g2sm2838313yhc.28.2011.01.28.12.41.39 6, 21 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 21 -- Fri, 28 Jan 2011 12:41:40 -0800 (PST) 6, 21 -- Message-Id: {D290C485-7F5E-45F1-B542-F8FEFBA0E7EC-at-ucdavis.edu} 6, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Confocal LSM: Quantification software? ImageJ 6, 21 -- Date: Fri, 28 Jan 2011 12:41:37 -0800 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
==============================Original Headers============================== 17, 28 -- From michael.anderson-at-nist.gov Fri Jan 28 15:13:10 2011 17, 28 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0SLD7Nq017047 17, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 15:13:09 -0600 17, 28 -- Received: from WSXGHUB2.xchange.nist.gov (wsxghub2.nist.gov [129.6.18.19]) 17, 28 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id p0SLDCwB015552 17, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 28 Jan 2011 16:13:12 -0500 17, 28 -- Received: from MBCLUSTER.xchange.nist.gov ([fe80::d479:3188:aec0:cb66]) by 17, 28 -- WSXGHUB2.xchange.nist.gov ([129.6.18.19]) with mapi; Fri, 28 Jan 2011 17, 28 -- 16:12:57 -0500 17, 28 -- From: "Anderson, Michael" {michael.anderson-at-nist.gov} 17, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 17, 28 -- Date: Fri, 28 Jan 2011 16:12:58 -0500 17, 28 -- Subject: RE: [Microscopy] Confocal LSM: Quantification software? ImageJ 17, 28 -- Thread-Topic: [Microscopy] Confocal LSM: Quantification software? ImageJ 17, 28 -- Thread-Index: Acu/LgWG7ETKLKAUTzyH3+igftiZtAAANZvw 17, 28 -- Message-ID: {7E80450DE7474E4881E405C742AC0DEA11DAAB5B7F-at-MBCLUSTER.xchange.nist.gov} 17, 28 -- References: {201101282057.p0SKveHC015550-at-ns.microscopy.com} 17, 28 -- In-Reply-To: {201101282057.p0SKveHC015550-at-ns.microscopy.com} 17, 28 -- Accept-Language: en-US 17, 28 -- Content-Language: en-US 17, 28 -- X-MS-Has-Attach: 17, 28 -- X-MS-TNEF-Correlator: 17, 28 -- acceptlanguage: en-US 17, 28 -- Content-Type: text/plain; charset="us-ascii" 17, 28 -- MIME-Version: 1.0 17, 28 -- X-NIST-MailScanner: Found to be clean 17, 28 -- X-NIST-MailScanner-From: michael.anderson-at-nist.gov ==============================End of - Headers==============================
I'm doing some testing today. Please bear with the likely of some test messages.
Nestor =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 9700 S. Cass Ave. Argonne, Illinois 60439 USA Tel: 530-NES-TORZ (530-637-8679) Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow of the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory President: Microscopy Society of America ===========================================
==============================Original Headers============================== 11, 24 -- From zaluzec-at-aaem.amc.anl.gov Sat Jan 29 09:53:54 2011 11, 24 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0TFrrN4014522 11, 24 -- for {microscopy-at-microscopy.com} ; Sat, 29 Jan 2011 09:53:53 -0600 11, 24 -- Received: from localhost (localhost [127.0.0.1]) 11, 24 -- by aaem.amc.anl.gov (Postfix) with ESMTP id D74DE2343B3B; 11, 24 -- Sat, 29 Jan 2011 09:53:52 -0600 (CST) 11, 24 -- X-Virus-Scanned: amavisd-new at aaem.amc.anl.gov 11, 24 -- Received: from aaem.amc.anl.gov ([127.0.0.1]) 11, 24 -- by localhost (aem005.amc.anl.gov [127.0.0.1]) (amavisd-new, port 10024) 11, 24 -- with ESMTP id tsveAIPV-hiB; Sat, 29 Jan 2011 09:53:52 -0600 (CST) 11, 24 -- Received: from [206.69.208.22] (msdvpn005.msd.anl.gov [130.202.251.5]) 11, 24 -- by aaem.amc.anl.gov (Postfix) with ESMTPA id 5D2C22343B30; 11, 24 -- Sat, 29 Jan 2011 09:53:51 -0600 (CST) 11, 24 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 11, 24 -- Content-Type: text/plain; charset=us-ascii 11, 24 -- Content-Transfer-Encoding: 7bit 11, 24 -- Subject: Administrivia: Testing today 11, 24 -- Date: Sat, 29 Jan 2011 09:53:49 -0600 11, 24 -- Message-Id: {270AF016-187C-4DDF-B8AD-5D8BE1C695D7-at-aaem.amc.anl.gov} 11, 24 -- Cc: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov} 11, 24 -- To: microscopy-at-microscopy.com 11, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 11, 24 -- X-Mailer: Apple Mail (2.1082) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reynolds-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reynolds-at-vt.edu Name: Bill Reynolds
Organization: Virginia Tech
Title-Subject: [Microscopy] Re: How close can a NMR machine be installed next to
Message: We have a FEG-STEM with a GIF that is located about 30 meters away from an NMR lab. Our microscope operates to the microscope manufacturer's resolution specs. As others have noted, what causes problems for TEMs are magnetic fields that change relatively quickly. These come from things like power lines, large motors, and electromagnetic contacts or switches. Static fields do not matter much at all because you can compensate for them (even when the field is associated with a ferromagnetic sample in the microscope). When our microscope was installed, the manufacturer measured the stray magnetic fields (the alternating fields that are easy to detect), but I don't think they even bothered measuring the static field. As an aside, the natural static field at the earth's surface is small, but it is not insignificant. Its magnitude varies from place to place, but it is in the vicinity of half a Gauss. All microscopes essentially work in a 500 milli Gauss static background.
The large fields associated with NMR instruments are also static, so they probably should not be considered equivalent to the "stray EMF fields" discussed in various microscope installation specs. In our lab, the magnet is a superconducting coil, and the weak fringes of the magnetic field extends to our microscope. However, since the field doesn't change (and we don't distort it by moving around large chunks of iron), our microscope does not notice the presence of the NMR. If the NMR magnet ever quenches or is turned off, we will probably be able to see a very small change in the microscope alignment, but that kind of event has not happened yet because the NMR magnet has been on continuously for a couple years.
I assume, rather than a simple chord on a 2D z slice, you want to measure a line between two points on different Z planes in the 3D z stack. If so also try and track down a recent version of the 3D software Volocity from Perkin Elmer [formally Improvision], I am sure there will be a few licences for the software about in one of the microscopy cores or research groups at the university. Volocity is pretty hot at 3D quantification, e.g. cellular component volumes etc.., and it shows them as pretty pictures & videos. Although expensive to buy, Volocity works well, gives good feedback on what it was measured, and has excellent email and telephone support in the UK, and I expect the same is true in the US. You will need to find a licenced user with at least the visualisation module, and probably the quantitation module [i.e. drawing tools], particularly if you want to take the image analysis further [these cost extra and not all users will have all the modules].
http://www.cellularimaging.com/products/volocity http://www.well.ox.ac.uk/_asset/file/volocity-manual.pdf [see line tool] http://www.cellularimaging.com/lab_of_the_month/detail.php?id=12
I'm sure there will be other microscope software that other Californian University groups may have that can do this as well, including ImageJ [although I can't think of a 3D line distance measurements ImageJ app, more volume rendering, but check the Fuji version], MetaMorph [also only available via an optional 3D module] and Image-Pro Plus [3D rendering and measurement].
If you find your XY pixel cordinates of the first and last point in the 3D confocal z-stack and know your approx. optical slice thickness and number of slices, I guess you could calculate/estimate the connecting line length pretty easily [you'd convert everything to pixels and then back to um]. There'd be errors in the length measurement, e.g. owing to perhaps 10 to 25% reduction in size due to fixation, possible distortion effects and estimation of Z optical distances.
e.g. calculate using http://www.analyzemath.com/Geometry_calculators/distance_midpoint_3D.html
Also see: Microscopy Image Analysis and Processing Software links at: http://www.well.ox.ac.uk/external-website-links
Regards
Keith
No commercial interest
-------------------------------------------------
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
Dear microscopists, I have been asked to reproduce an apoptosis assay whereby cells in a dish cells are exposed to UVB light and then screened 8 to 36 hours later. Does anybody have experience with this who could give us some tips? May we use UVA, as in the UV lamp on the microscope, or do you have suggestions what type of lab might have a UVB lamp available? Thank you. Sincerely, Michael
_________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 6, 29 -- From Michael.Cammer-at-med.nyu.edu Sun Jan 30 19:25:32 2011 6, 29 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0V1PWoF009546 6, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 30 Jan 2011 19:25:32 -0600 6, 29 -- X-IronPort-Anti-Spam-Filtered: true 6, 29 -- X-IronPort-Anti-Spam-Result: ApsEANSdRU0KkyXk/2dsb2JhbAClaLBdiGiDEYI9BIUT 6, 29 -- X-IronPort-AV: E=Sophos;i="4.60,402,1291611600"; 6, 29 -- d="scan'208";a="113355490" 6, 29 -- Received: from msgwsdcpht62.nyumc.org ([10.147.37.228]) 6, 29 -- by mail4.nyumc.org with SMTP; 30 Jan 2011 20:25:31 -0500 6, 29 -- Received: from MSGWSDCPMB06.nyumc.org ([10.147.55.93]) by 6, 29 -- MSGWSDCPHT62.nyumc.org ([10.147.37.228]) with mapi; Sun, 30 Jan 2011 20:25:31 6, 29 -- -0500 6, 29 -- From: "Cammer, Michael" {Michael.Cammer-at-med.nyu.edu} 6, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 29 -- Date: Sun, 30 Jan 2011 20:25:31 -0500 6, 29 -- Subject: how to induce apoptosis for in vitro microscopy? 6, 29 -- Thread-Topic: how to induce apoptosis for in vitro microscopy? 6, 29 -- Thread-Index: AQHLwOW/gnV38X+oNUGrJiYV3UT/IQ== 6, 29 -- Message-ID: {725986F6C4F57449A27E7D10B49F82B3151350C7CA-at-MSGWSDCPMB06.nyumc.org} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- acceptlanguage: en-US 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0V1PWoF009546 ==============================End of - Headers==============================
Dear All, I would like to do some work on lichen. Now I got access to a huge herbarium collection of lichen. What it do any good to use these room-dried specimen, moisture them, fix and go through ethanol to critcal point drying? Any experience with this out there?
Best wishes, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ian.maclaren-at-glasgow.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ian.maclaren-at-glasgow.ac.uk Name: Ian MacLaren
Organization: University of Glasgow
Title-Subject: [Filtered] EMAG2011 - Abstract Submission Open
Message: EMAG2011 - Quantifying the Nanoworld September 6-9 2011 University of Birmingham, UK
Call for abstracts
The biennial EMAG conference will this year be held at the University of Birmingham and promises to be an excellent meeting. Highlights of the programme will include plenary talks from Dr Frances Ross, Prof. Knut Urban and Prof. Richard Henderson, together with an exciting range of invited talks giving a snapshot of many current directions in electron microscopy and its applications. There will also be plenty of opportunity at this meeting to present papers orally or as posters, and research students are especially encouraged to present their work at this meeting. Scientific sessions are expected to include:
* Nanofabrication and scanning electron microscopy * Modelling and quantification * Advances in imaging and spectroscopy techniques * Applications of high resolution imaging and spectroscopy * Functional materials * Nanomaterials * Structural materials * Biosciences, biomaterials and soft matter * Earth and environmental sciences * In-situ electron microscopy Abstract submission is now open via the conference website at:
http://www.emag-iop.org/ and the closing date for abstract submission is 2 March 2011. Further information about the conference is also available from the same website. We look forward to seeing many of you in Birmingham in September.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vcsolomon-at-ysu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: vcsolomon-at-ysu.edu Name: Virgil C. Solomon
Organization: Youngstown State University
Title-Subject: [Filtered] Postdoctoral Research Position
Message: A postdoctoral research position is available at Youngstown State University to study ceramic-metallic composites obtained by reactive melt infiltration. More specifically, the successful candidate will use transmission and scanning electron microscopy methods to characterize micro- and nanostructural features of the composite materials. Candidates should have a Ph.D. in materials science, chemistry, physics or related engineering discipline. The position requires extensive experience in analytical scanning and transmission electron microscopy and sample preparation techniques; additional experience working with focused ion beam is preferred. Responsibilities of the successful candidate will include planning and performing experiments, processing and evaluating the results, and preparing scientific reports and journal publications. This position provides the opportunity to work in a new, fully-equipped, state-of-the-art electron microscopy facility which opened at YSU in 2010.
More information available at http://www.ysu.edu/hr/positions.shtml
Interested candidates can apply by email or by sending a cover letter, CV with a complete list of publications, and contact information of 3 professional references to: Dr. Tim Wagner or Dr. Virgil Solomon Youngstown State University One University Plaza Youngstown OH 44555-0001 E-mails: trwagner-at-ysu.edu; vcsolomon-at-ysu.edu
it's not my particular area of expertise but I do recall that some of our researchers used to "revitalise" their dried lichen samples by simply putting them on a damp filter paper in a petri-dish. If you think about it, this is probably what happens in nature (except for the filter paper etc).
I seem to recall that this process worked with most specimens but depended on the original drying, storage and presumably the resilience of the fungi and algae symbionts.
Good luck
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: stefan.diller-at-t-online.de
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both steven.samuelsson-at-sri.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: steven.samuelsson-at-sri.com Name: Steven Samuelsson Organization: SRI International, Inc.
Message: We recently acquired a vintage Reichert- Jung, Cryocut 1800. Unfortunately, it is missing much of the knife holder assemble. Does anyone on the listserver have these parts and are willing to sell or surplus them? The unit is in good condition and should serve us well. Thanks so much.
Steve -- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball Institute of Biomolecular Medicine
Title-Subject: [Filtered] Type K(?) insert question
Message: We want to built a custom type K (?), 110 X 160 mm with rounded corners, insert for our Zeiss microscope stage that will allow us to insert another stage insert approximately 2 cm higher than the current stage.
Does anyone know where we can get drawings of the insert that we modify for a machine shop?
Or does anyone know if such a stage height extender already exists?
Thank you very much.
Sincerely,
Michael
________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
The Hooke College of Applied Sciences, located in Westmont, IL, is offering the following electron microscopy short courses:
March 7 to 11- Scanning Electron Microscopy
March 15 to 17 - Transmission Electron Microscopy
In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below:
http://www.hookecollege.com/
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 15, 24 -- From eschumacher-at-mccrone.com Tue Feb 1 09:42:05 2011 15, 24 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 15, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11Fg4WM011955 15, 24 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 09:42:05 -0600 15, 24 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 15, 24 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Tue, 1 Feb 2011 09:42:02 15, 24 -- -0600 15, 24 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 15, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 15, 24 -- Date: Tue, 1 Feb 2011 09:42:02 -0600 15, 24 -- Subject: Short Course Announcement: SEM and TEM 15, 24 -- Thread-Topic: Short Course Announcement: SEM and TEM 15, 24 -- Thread-Index: AcvCJpHK9zItNqU0QweSJMKLY4Mzdw== 15, 24 -- Message-ID: {874B1DB532886444A60A015EE363493F4849CECF32-at-mccronemsg07.tmg.mccrone.com} 15, 24 -- Accept-Language: en-US 15, 24 -- Content-Language: en-US 15, 24 -- X-MS-Has-Attach: 15, 24 -- X-MS-TNEF-Correlator: 15, 24 -- x-vipre-scanned: 05BFDCEF00205105BFDE3C 15, 24 -- acceptlanguage: en-US 15, 24 -- Content-Type: text/plain; charset="us-ascii" 15, 24 -- MIME-Version: 1.0 15, 24 -- Content-Transfer-Encoding: 8bit 15, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p11Fg4WM011955 ==============================End of - Headers==============================
Dear Stefan, I have no specific experience with lichen but I suspect that whatever structural damage occurred by air drying cannot be repaired by rehydration. I bet you can take small sample of the herbarium specimen and put it on a stub and examine directly. I think you can test it first in a vacuum chamber (for example of a good coater) and see if it outgasses much. Probably it won't. Insofar as lichens air dry in nature and plant and fungal cell walls are tough, it is reasonable to examine air dried material in lieu of fresh samples.
Clearly if as Malcolm Haswell suggests the herbarium specimen is still living, then that gives you the chance to work with fresh material. But I wonder what the odds of that are?
As ever Tobias Baskin
} } } Dear All, } I would like to do some work on lichen. Now I got access to a huge } herbarium collection of lichen. } What it do any good to use these room-dried specimen, moisture them, } fix and go through ethanol to critcal point drying? } Any experience with this out there? } } Best wishes, } Stefan } } -- } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.stefan-diller.com } www.elektronenmikroskopie.info } www.assisi.de } www.zwillingsprojekt.de } Anfahrt: //Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } =
We often neglect to consider that when something has already been dried 'naturally' there may be no reason the redo the process 'un-naturally'. Especially in the cases of organisms that can survive dehydration by re-animating when re-hydrated. There is a roundworm in S. America that survives complete dessication in the dried mud - waiting for reanimation in the next rainy season. (Could NOT quickly find a reference, but I first saw this in a text - when I was young - in the early 1960's.)
This is especially true, if you have a variable pressure SEM (ESEM) at hand.
Further, after critical point drying, we also fail to recognize that the 'almost completely dry' natural material has been remade into a quite hygroscopic material that will rehydrate while we carry it to the microscope or coater (evacuate (shrinkage) and coat, then re-pressurize, hydrate and stretch!). All of this was invented to permit us to view free-standing microvilli as well as other delicate cell surface features.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de] Sent: Tuesday, February 01, 2011 7:32 AM To: Monson, Frederick
Dear All, I would like to do some work on lichen. Now I got access to a huge herbarium collection of lichen. What it do any good to use these room-dried specimen, moisture them, fix and go through ethanol to critcal point drying? Any experience with this out there?
Best wishes, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Hi all, Does anyone have a favorite TEM protocol for fixing mouse cornea that they'd be willing to share? The tissue we have is infected with a fungus so we'd like to have good preservation of both. TIA and my best, Beth
Beth Richardson EM Lab Coordinator Plant Biology Dept. University of Georgia Athens, GA 30602
==============================Original Headers============================== 5, 19 -- From beth-at-plantbio.uga.edu Tue Feb 1 13:55:21 2011 5, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11JtIPe001424 5, 19 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 13:55:20 -0600 5, 19 -- Received: from [128.192.26.46] ([128.192.26.46]) 5, 19 -- (authenticated user beth-at-plantbio.uga.edu) 5, 19 -- by dogwood.plantbio.uga.edu 5, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 5, 19 -- for microscopy-at-microscopy.com; 5, 19 -- Tue, 1 Feb 2011 14:55:14 -0500 5, 19 -- Message-Id: {1135C2EB-B555-4DD6-833D-1D690BEB1955-at-plantbio.uga.edu} 5, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 5, 19 -- Content-Transfer-Encoding: 7bit 5, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 5, 19 -- Subject: fixing mouse cornea 5, 19 -- Date: Tue, 1 Feb 2011 14:55:14 -0500 5, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
The editor of a forthcoming Wiley-Blackwell book on Biofouling Methods is looking for someone to write a chapter on electron microscopy methods. This is expected to be a brief literature review, with an introduction about methods and some actual protocols, designed for beginners in the field. If you are interested, please contact Sergey Dobretsov at sergey_dobretsov-at-yahoo.com
Aloha from sunny and warm Hawaii (had to rub it in), Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Tue Feb 1 15:06:27 2011 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11L6Q2R019293 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 15:06:26 -0600 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p11L6PH1008231 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 11:06:25 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p11L6O1Q008227 4, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 11:06:25 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Tue, 1 Feb 2011 11:06:24 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Need a chapter on EM for a book 4, 20 -- Message-ID: {Pine.GSO.4.64.1102011104350.5800-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
As suggested already, if you have access to a VP-SEM, all you need to do is put a piece on a stub and image. And sometimes BSE is better than SE, or a combination of the two can work well. And in a VP-SEM, you don't need to worry about outgassing so much.
After that, you could try looking at a hydrated specimen in VP mode, with water as the gas, if your SEM has that capacity.
The entomologists across the road are big fans of the VP-SEM, because it means they can now look at details of their precious type specimens, especially the very tiny ones, none of which are allowed to be coated with anything.
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 1/02/11 11:29 PM, "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } I would like to do some work on lichen. Now I got access to a huge herbarium } collection of lichen. } What it do any good to use these room-dried specimen, moisture them, fix and } go through ethanol to critcal point drying? } Any experience with this out there? } } Best wishes, } Stefan
==============================Original Headers============================== 11, 42 -- From prvs=006eee44e=Rosemary.White-at-csiro.au Tue Feb 1 15:34:52 2011 11, 42 -- Received: from vic-ironport-ext.csiro.au (vic-mx-ext.csiro.au [150.229.64.36]) 11, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p11LYp8t003304 11, 42 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 15:34:52 -0600 11, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 11, 42 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 11, 42 -- s=email; t=1296596092; x=1328132092; 11, 42 -- h=date:subject:from:to:cc:message-id:in-reply-to: 11, 42 -- mime-version:content-transfer-encoding; 11, 42 -- z=Date:=20Wed,=202=20Feb=202011=2008:34:49=20+1100 11, 42 -- |Subject:=20Re:=20[Microscopy]=20SEM=20prep=20of=20lichen 11, 42 -- =20from=20a=20herbarium|From:=20Rosemary=20White=20 {rosem 11, 42 -- ary.white-at-csiro.au} |To:=20"stefan.diller-at-t-online.de"=20 { 11, 42 -- stefan.diller-at-t-online.de} |CC:=20 {microscopy-at-microscopy.c 11, 42 -- om} |Message-ID:=20 {C96EC7A9.21197%rosemary.white-at-csiro.au 11, 42 -- } |In-Reply-To:=20 {201102011229.p11CTq4x016236-at-ns.microsco 11, 42 -- py.com} |MIME-Version:=201.0|Content-Transfer-Encoding:=20 11, 42 -- 7bit; 11, 42 -- bh=9TRDFqpqRN1bdVNvOFDlMx5U/Gk9blzeTG1TonXHvE4=; 11, 42 -- b=HWF8t3R2/h16Lp0f9PVok799pYD36NMLIe+idDXMmZjQSbn9j9exnpks 11, 42 -- RxlMhigmFaLwiWqZydWatdvJzGtN2LHOckHOaermHc/CfO7EL5IFEYGpS 11, 42 -- lUV+OWtYBuaO2uP; 11, 42 -- X-IronPort-AV: E=Sophos;i="4.60,411,1291554000"; 11, 42 -- d="scan'208";a="54545709" 11, 42 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127]) 11, 42 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 02 Feb 2011 08:34:50 +1100 11, 42 -- Received: from [152.83.195.117] (152.83.195.117) by 11, 42 -- exvic-htca02.nexus.csiro.au (138.194.81.127) with Microsoft SMTP Server (TLS) 11, 42 -- id 8.1.436.0; Wed, 2 Feb 2011 08:34:22 +1100 11, 42 -- User-Agent: Microsoft-Entourage/12.28.0.101117 11, 42 -- Date: Wed, 2 Feb 2011 08:34:49 +1100 11, 42 -- Subject: Re: [Microscopy] SEM prep of lichen from a herbarium 11, 42 -- From: Rosemary White {rosemary.white-at-csiro.au} 11, 42 -- To: "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de} 11, 42 -- CC: {microscopy-at-microscopy.com} 11, 42 -- Message-ID: {C96EC7A9.21197%rosemary.white-at-csiro.au} 11, 42 -- Thread-Topic: [Microscopy] SEM prep of lichen from a herbarium 11, 42 -- Thread-Index: AcvCC6tpNkA5cKiQSneyVPbN+dFh2wATC7YU 11, 42 -- In-Reply-To: {201102011229.p11CTq4x016236-at-ns.microscopy.com} 11, 42 -- MIME-Version: 1.0 11, 42 -- Content-Type: text/plain; charset="US-ASCII" 11, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have been offered a demonstration in our laboratory of the Milestone "KOS EM" tissue processor. Has anyone encountered this product before? Perhaps some of you have had experience with other Microwave tissue processors that you may wish to share.
Thanks in advance Naomi
Naomi McCallum Supervising Scientist, EM Unit, Anatomical Pathology Pathology Queensland _________________________________________________ Clinical and Statewide Services Division| Queensland Health
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 9, 25 -- From prvs=101452c9f9=naomi_mccallum-at-health.qld.gov.au Tue Feb 1 21:00:53 2011 9, 25 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1230q5x025783 9, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 21:00:53 -0600 9, 25 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 9, 25 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id p1230BBR007259 9, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 13:00:11 +1000 9, 25 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 9, 25 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 9, 25 -- with ESMTP id 9, 25 -- {T9af2de31c6c0a80335d48-at-gwd-mail06.remote.health.qld.gov.au} for 9, 25 -- {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 13:00:11 +1000 9, 25 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 9, 25 -- Novell_GroupWise; Wed, 02 Feb 2011 13:00:10 +1000 9, 25 -- Message-Id: {4D495547.88BE.00AA.0-at-health.qld.gov.au} 9, 25 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 9, 25 -- Date: Wed, 02 Feb 2011 12:59:54 +1000 9, 25 -- From: "Naomi McCallum" {naomi_mccallum-at-health.qld.gov.au} 9, 25 -- To: {Microscopy-at-microscopy.com} 9, 25 -- Subject: KOS EM (Milestone) Microwave Tissue Processor 9, 25 -- Mime-Version: 1.0 9, 25 -- Content-Type: text/plain; charset="us-ascii" 9, 25 -- Content-Disposition: inline 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1230q5x025783 ==============================End of - Headers==============================
Dear Justin, We can supply small quantities of Pt-Ir STM tips at a price much lower than "$200 per tip". The tips we have were manufactured at Digital Instruments for use with their STM. Please contact me offline and share details of the wire size requirements of the Easyscan stm.
regards, Don ============================================= Don Chernoff, Ph.D., President Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com 3250 N. Post Rd., Ste. 120 Voice: 317-895-5630 INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada) web: http://www.asmicro.com Fax: 317-895-5652 [business activities: analytical services in AFM, AFM probes, consulting, training, calibration and test specimens, calibration and measurement software, used NanoScope equipment.] 2009-2010 is our 20th year in business! ============================================= ----- Original Message ----- From: kraftpiano-at-gmail.com To: donc-at-asmicro.com Sent: Friday, January 21, 2011 12:10 AM Subject: [a] [Microscopy] STM tip advice.
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
Thanks,
Justin A. Kraft
==============================Original Headers============================== 6, 37 -- From kraftpiano-at-gmail.com Thu Jan 20 23:08:26 2011 6, 37 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p0L58Q25003006 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 23:08:26 -0600 6, 37 -- Received: by yxl31 with SMTP id 31so469145yxl.0 6, 37 -- for {microscopy-at-microscopy.com} ; Thu, 20 Jan 2011 21:08:26 -0800 (PST) 6, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 6, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 6, 37 -- bh=0UOwJelpZ6QJtvR6h504fJNBtAxqg11VNSbyg5wJQ74=; 6, 37 -- b=CKvCVRNoVTsFwRnLpU5lCXf8YvPMxH1N3dS7ys8UPf3yiqhPDWGbMo0obmG88cgOgo 6, 37 -- XZULU3FfCYHsarDBM9jexEC0W3v5YP7EFDMCVYhQlXG/1kA7Yhd73anJHaX3ZIz+H7uz 6, 37 -- JgDVe+ofiVTeCAAmXGLSf7iUKv6XvcnYIVDww= 6, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 37 -- d=gmail.com; s=gamma; 6, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 6, 37 -- :to:mime-version:x-mailer; 6, 37 -- b=PR17AmFsaRKhUKvApaUTF9Rk1PZybTM2uGPbnyI+JSfekwjDyixYQTfQWeBGOPBeUR 6, 37 -- jtKbSKxwwTpYoyFNLlhFZrNVcU8rUujb+K2L3feJgIONXADzcGx34Xj28IMbBnWZHLwQ 6, 37 -- 1EKfQrQ/cpSgDSCYg9bEB0VVr0WIDudjYmUZI= 6, 37 -- Received: by 10.151.68.12 with SMTP id v12mr198668ybk.387.1295586505917; 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 6, 37 -- by mx.google.com with ESMTPS id u10sm82704yba.13.2011.01.20.21.08.24 6, 37 -- (version=TLSv1/SSLv3 cipher=RC4-MD5); 6, 37 -- Thu, 20 Jan 2011 21:08:25 -0800 (PST) 6, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 6, 37 -- Content-Type: text/plain; charset=us-ascii 6, 37 -- Subject: STM tip advice. 6, 37 -- Date: Fri, 21 Jan 2011 00:08:23 -0500 6, 37 -- Message-Id: {EA4CFA28-451C-4E4D-A543-EEA026824B89-at-gmail.com} 6, 37 -- To: microscopy-at-microscopy.com 6, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 37 -- X-Mailer: Apple Mail (2.1082) 6, 37 -- Content-Transfer-Encoding: 8bit 6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p0L58Q25003006 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 37 -- From donc-at-asmicro.com Tue Feb 1 21:16:05 2011 15, 37 -- Received: from nm18.bullet.mail.sp2.yahoo.com (nm18.bullet.mail.sp2.yahoo.com [98.139.91.88]) 15, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p123G45i006759 15, 37 -- for {microscopy-at-microscopy.com} ; Tue, 1 Feb 2011 21:16:05 -0600 15, 37 -- Received: from [98.139.91.69] by nm18.bullet.mail.sp2.yahoo.com with NNFMP; 02 Feb 2011 03:16:04 -0000 15, 37 -- Received: from [98.139.91.51] by tm9.bullet.mail.sp2.yahoo.com with NNFMP; 02 Feb 2011 03:16:04 -0000 15, 37 -- Received: from [127.0.0.1] by omp1051.mail.sp2.yahoo.com with NNFMP; 02 Feb 2011 03:16:04 -0000 15, 37 -- X-Yahoo-Newman-Id: 507173.81244.bm-at-omp1051.mail.sp2.yahoo.com 15, 37 -- Received: (qmail 69479 invoked from network); 2 Feb 2011 03:16:04 -0000 15, 37 -- Received: from asm15 (donc-at-98.226.79.148 with login) 15, 37 -- by smtp105.sbc.mail.gq1.yahoo.com with SMTP; 01 Feb 2011 19:16:00 -0800 PST 15, 37 -- X-Yahoo-SMTP: 9Mfb0weswBDvm9orSc8mC.8UiMpq8hJTCpyHEMOqu04urw-- 15, 37 -- X-YMail-OSG: OwyFFLQVM1l627x6tANLqy9dAhznmGz_RYKL37RoMqudbgD 15, 37 -- sjMM_zwbMpL4jA.5BJfq0enNJKlVVzd2uPKjZXt791Ujqn0GzXGLw1ZDNG5R 15, 37 -- K.FNTnLyj8CQ1l.J7.e4bvJR7FEgciIkD6L.9aI0MbUiyjux414wQSUT1jzS 15, 37 -- JCEM9JVgPHEt_gkSB9qOFeq5TUhFJDPIL80GCC2LoZQ1u9E8FvKe64DRZVLz 15, 37 -- GL43xGl_vYp4W4YZ7QK2zSSqYeEtHTA6jfWwDI6rBCkCYWyCe.nI1A4zq1z1 15, 37 -- buvs3WvOXPXjz1blFJP8WHeJoDmFSCfWzkU0Gzi_T6PTHZBFiDPtk1GKcO5Z 15, 37 -- cs52bx8rES9VmSyqnIqOEkGl_DKkatNWZOcUBtB.MrBc- 15, 37 -- X-Yahoo-Newman-Property: ymail-3 15, 37 -- Message-ID: {F04A2847377549D2AF7EA247052A4897-at-asm15} 15, 37 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com} 15, 37 -- To: {kraftpiano-at-gmail.com} 15, 37 -- Cc: "Microscopy List" {microscopy-at-microscopy.com} 15, 37 -- References: {201101210510.p0L5A0cD006982-at-ns.microscopy.com} 15, 37 -- Subject: Re: [a] [Microscopy] STM tip advice. - Commercial reply 15, 37 -- Date: Tue, 1 Feb 2011 22:14:14 -0500 15, 37 -- MIME-Version: 1.0 15, 37 -- Content-Type: text/plain; 15, 37 -- format=flowed; 15, 37 -- charset="iso-8859-1"; 15, 37 -- reply-type=original 15, 37 -- Content-Transfer-Encoding: 7bit 15, 37 -- X-Priority: 3 15, 37 -- X-MSMail-Priority: Normal 15, 37 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 15, 37 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 ==============================End of - Headers==============================
Sorry for the delay in response to this topic, but due to an error on my on my part my previous communication went astray. For those who may be interested in the history of ultramicrotomy I do have a copy of an article in PDF form which traces the development of the ultramicrotome from "wedge" sections in the late thirties where the thin end of the wedge was (hopefully) transparent to electrons, through the high speed era (actually up to 57000 rpm), overcoming embedding limitations and finally discussing the first generation of commercial instruments.
Please contact me on terry.cooper-at-btinternet.com and I can attach the missive,
Best regards
Terry Cooper TAAB Laboratories Equipment 3 Minerva House, Calleva Park Aldermaston, Berks, RG7 8NA, England Tel ++44(0)118 981 7775 Fax ++44(0)118 981 7881 e-mail sales-at-taab.co.uk www.taab.co.uk
==============================Original Headers============================== 6, 39 -- From terry.cooper-at-btinternet.com Wed Feb 2 05:39:05 2011 6, 39 -- Received: from nm5.bullet.mail.ird.yahoo.com (nm5.bullet.mail.ird.yahoo.com [77.238.189.62]) 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p12Bd4GD004670 6, 39 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 05:39:04 -0600 6, 39 -- Received: from [77.238.189.57] by nm5.bullet.mail.ird.yahoo.com with NNFMP; 02 Feb 2011 11:39:03 -0000 6, 39 -- Received: from [212.82.108.246] by tm10.bullet.mail.ird.yahoo.com with NNFMP; 02 Feb 2011 11:39:03 -0000 6, 39 -- Received: from [127.0.0.1] by omp1011.mail.ird.yahoo.com with NNFMP; 02 Feb 2011 11:39:03 -0000 6, 39 -- X-Yahoo-Newman-Id: 779398.69037.bm-at-omp1011.mail.ird.yahoo.com 6, 39 -- Received: (qmail 86766 invoked from network); 2 Feb 2011 11:39:03 -0000 6, 39 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 39 -- s=s1024; d=btinternet.com; 6, 39 -- h=DKIM-Signature:Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X-MSMail-Priority:X-Mailer:X-MimeOLE; 6, 39 -- b=IsObn+QLugwXVehYYiLw4s49xXwDqDZnhG9NNU4bIdzkofRt1VP0unu2tL6xZl2Sn4B0P6skvnc4oU3/Vz+MSfrTvXJJP0vwthYxKuegao2YJYJKxBY31StZ6EetQNgRNKsM9i+2WRIGannNqe0McTHQMOCZ8dn6/wwzQ7mHXrA= ; 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=btinternet.com; s=s1024; t=1296646743; bh=oWdodXDAladTRTeAY1E2NNtKCYK+97yXwv8tXphMquI=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X-MSMail-Priority:X-Mailer:X-MimeOLE; b=VKuj8pFNykoHwXnX54sk3fQwJkOZuoFXszGFPDSrEBKHHYq1DhiRlqIpTJ0MbhCZ90mrkMGJGx7jvganojRhWDRuoc0kwwPzAnbIlk/bme1OYZj+omRhbm7WR6+jbygWxaJiNoCrMyI3euTEnUXnS9XDuEToKBTrHyxyDEMCDBc= 6, 39 -- Received: from Pioneer (terry.cooper-at-94.30.34.8 with login) 6, 39 -- by smtp817.mail.ird.yahoo.com with SMTP; 02 Feb 2011 11:39:00 +0000 GMT 6, 39 -- X-Yahoo-SMTP: pVuM3eCswBBmTWesC4KmNptnh5To2FPTm29gm3HoxqLR7BH.yfiY1w-- 6, 39 -- X-YMail-OSG: NozSGW4VM1m6bRR8Zaj1i1Ns2oY8lipJOBHfz_emqEZD7Mz 6, 39 -- zVSXR5KJ0BzFhcVKMQO.WbUOmXArrNSCPPZWh8tM6axZRAE73cHQ.ln61SO9 6, 39 -- DQkj6etKzZY_ajLLLC.zUoMqYjs2wMLSAAl2QujHC5Kb13GH4ItZm4d3Q64m 6, 39 -- J6zDCnwQouOZrxCbBg4v2t3bwm5d9oDgemTErzM7csvNyzX8QVdqPncU6CZ8 6, 39 -- qx4oeuCZHTwoR4VAvY0LcYBzAhjEUfvcns1bVlRfFM_eK5_EWeFSz6DHdKqE 6, 39 -- lLKPz1rsDlBI- 6, 39 -- X-Yahoo-Newman-Property: ymail-3 6, 39 -- Message-ID: {8F6E8C47CA0E40BB89DFF1D968EE4462-at-Pioneer} 6, 39 -- From: "terry cooper" {terry.cooper-at-btinternet.com} 6, 39 -- To: {microscopy-at-microscopy.com} 6, 39 -- Subject: Ultramicrotome history 6, 39 -- Date: Wed, 2 Feb 2011 11:39:22 -0000 6, 39 -- MIME-Version: 1.0 6, 39 -- Content-Type: text/plain; 6, 39 -- format=flowed; 6, 39 -- charset="iso-8859-1"; 6, 39 -- reply-type=original 6, 39 -- Content-Transfer-Encoding: 7bit 6, 39 -- X-Priority: 3 6, 39 -- X-MSMail-Priority: Normal 6, 39 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 6, 39 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] SEM sample storing
Message: Hello all, One of our EM lab users asked me a question: what is the best way to store SEM samples? I know you'll all say that the best is to process and coat first. He wants to store them in 2% glutaraldehyde and process later since he does not have time at the moment. What is your experience? I am not an expert in SEM. Thank you Dorota Login Host: 137.149.102.148 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 24 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 2 07:55:51 2011 7, 24 -- Received: from hiltonsmtp.worldspice.net (hiltonsmtp.worldspice.net [216.37.94.58]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12DtpQn024776 7, 24 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 07:55:51 -0600 7, 24 -- Received: (qmail 2812 invoked by uid 0); 2 Feb 2011 13:55:50 -0000 7, 24 -- Received: by simscan 1.4.0 ppid: 2527, pid: 2784, t: 0.4412s 7, 24 -- scanners: clamav: 0.94.1/m:50/d:9101 spam: 3.2.5 7, 24 -- X-Spam-Checker-Version: SpamAssassin 3.2.5-wspice_748 (2008-06-10) on 7, 24 -- hiltoncluster1.hiltonsmtp.worldspice.net 7, 24 -- X-Spam-Level: 7, 24 -- X-Spam-Status: No, score=0.9 Bayes=0.5 required=6.5 autolearn=disabled version=3.2.5-wspice_748 report= 7, 24 -- * 0.9 CS_796 CS_796 7, 24 -- Received: from unknown (HELO NestorPwrBk.local) (zaluzec-at-12.97.46.2) 7, 24 -- by hiltoncluster01.worldspice.net with ESMTPA; 2 Feb 2011 13:55:50 -0000 7, 24 -- Message-ID: {4D49625B.7080502-at-microscopy.com} 7, 24 -- Date: Wed, 02 Feb 2011 05:55:39 -0800 7, 24 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 24 -- Reply-To: wadowska-at-upei.ca 7, 24 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 24 -- MIME-Version: 1.0 7, 24 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 24 -- Subject: viaWWW:SEM sample storing 7, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 24 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Dorota To my experience specimens can stay in glutar for an indefinite time. I have processed samples after } 3 years stored in glutar inside the fridge, and they looked same as others being processed within a few days. In rare cases I've seen mushrooms developing in samples that stayed only a few weeks in glutar. There I suspect glutar was old, samples were dirty from the beginning, or some other unedintified factor was present. If you process and coat the specimens I think it is better to view them soon, otherwise can get humidified and morphology deteriorates. In conclusion I think it is not a bad idea to have your samples staying in glutar until you have the time to view them. With best wishes yorgos
----- Original Message ----- X-from: {microscopylistserver-noreply-at-microscopy.com} To: {eikonika-at-otenet.gr} Sent: Wednesday, February 02, 2011 3:05 PM
Dear Colleagues,
Openings for Engineers & Technicians in Physical Failure Analysis (PFA) are available in Global Foundries, R&D Center at East Fishkill, NY.
Engineers should be strong in FIB & SEM / basic TEM & device physics. Industry experience with device / wafer-fab helpful.
Technicians should have extensive hands-on skills in various TEM sample-prep techniques, e.g., delayering / mechanical polishing / FIB / CLM, etc. Previous experience at high-throughput environments preferred, but not a requirement.
Applicants should demonstrate unrestricted employment eligibility in USA, which usually requires US citizenship or green card.
If interested, please send a cover letter and resume, "offline", to wayne.zhao-at-globalfoundries.com .
Wayne
------------------------------------------- Wayne Zhao, PhD Technical Lead of PFA Team Sr. Member of Technical Staff R & D Center at East Fishkill, NY Global Foundries -------------------------------------------
==============================Original Headers============================== 8, 30 -- From waynezhao.microscopy-at-gmail.com Wed Feb 2 08:50:25 2011 8, 30 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 8, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12EoP2w020535 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 2 Feb 2011 08:50:25 -0600 8, 30 -- Received: by iyi20 with SMTP id 20so32846iyi.0 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 02 Feb 2011 06:50:25 -0800 (PST) 8, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 30 -- d=gmail.com; s=gamma; 8, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 8, 30 -- :content-type; 8, 30 -- bh=/oN/9ZO7QsEHh+aUY1BNcdHKRMpbPqPh+ne5ae2/z+M=; 8, 30 -- b=Tpt+PSVS6TaXldFBBKDP8Gl+SWNYwUjf1jjAYAEONSr/Zucs25a5KgTdTh8+MZC1Zt 8, 30 -- IgANy1lY0CopGbMpbilcJPut8YZQDdnPz0U1k6zgjwlwYEQW1vZv0bSoW143hwSSt+4X 8, 30 -- 1gB4DuRho6OJrMXJrv6c0oPeSyt0D04BWESQI= 8, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 8, 30 -- d=gmail.com; s=gamma; 8, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 8, 30 -- b=fqfOc6QEDlB5ll8UYiBu4DXDtKBZwqLTRZzLCfXswAsORwOz3pZ9XyOtNqpw43PMzM 8, 30 -- 6DzfHLZibP9k36ettbcVySVjqO7tmCa3dzNdB68ML7fhtej59BUPmF6SXRzQ0uWRfm+C 8, 30 -- QcifT15WiufTxj423Gv8HHEaQivrgg/SZR9vo= 8, 30 -- MIME-Version: 1.0 8, 30 -- Received: by 10.42.169.68 with SMTP id a4mr11105516icz.525.1296658225169; Wed, 8, 30 -- 02 Feb 2011 06:50:25 -0800 (PST) 8, 30 -- Received: by 10.42.230.197 with HTTP; Wed, 2 Feb 2011 06:50:25 -0800 (PST) 8, 30 -- Date: Wed, 2 Feb 2011 09:50:25 -0500 8, 30 -- Message-ID: {AANLkTikmeH_qYwc+jC1KBt813kC-H1cM4_+W=SvA8hSw-at-mail.gmail.com} 8, 30 -- Subject: PFA Engineer & Technicians openings at Global Foundries 8, 30 -- From: Wayne Zhao {waynezhao.microscopy-at-gmail.com} 8, 30 -- To: Microscopy-at-Microscopy.Com 8, 30 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bbandli-at-d.umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bbandli-at-d.umn.edu Name: Bryan Bandli
Organization: University of Minnesota, Duluth
Title-Subject: [Filtered] HMDS, How does it work...
Message: Hi All,
I'm looking for a reference to explain just how/why HMDS works to dry specimens for SEM observation. There are plenty of references showing that it does, in fact, work and produces good results in many cases but I have yet to find an explanation as to the "why". Is it that HMDS has the right physical properties (surface tension) to evaporate without causing drying artifacts?
Or, is there something about the silanization of the sample that helps make it more robust, or both, or am I completely lost (quite likely)?
Bryan, I believe it has to do with very low surface tension. It also doesn't work well across the board. I understand that it works well with insects, but one of my sons did a science fair project comparing dried, freeze dried and HMDS treated green pepper. In his case the HMDS didn't appear to be any better than drying. His best results were from plunging small pieces into acetone cooled with a Peltier cooler, then transferring to another Peltier cooler in a vacuum evaporator until dehydrated. He was not able to compare it to CPD prep as we didn't have the requisite CO2. Obviously, the plunge freezing was not done at typical temperatures, so there was probably ice damage in those samples, but they looked the best of the bunch.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, February 02, 2011 10:02 AM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bbandli-at-d.umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bbandli-at-d.umn.edu Name: Bryan Bandli
Organization: University of Minnesota, Duluth
Title-Subject: [Filtered] HMDS, How does it work...
Message: Hi All,
I'm looking for a reference to explain just how/why HMDS works to dry specimens for SEM observation. There are plenty of references showing that it does, in fact, work and produces good results in many cases but I have yet to find an explanation as to the "why". Is it that HMDS has the right physical properties (surface tension) to evaporate without causing drying artifacts?
Or, is there something about the silanization of the sample that helps make it more robust, or both, or am I completely lost (quite likely)?
Although I have HMDS and have used it, I'm no expert.
That said,
given the following pieces, I'd say the lower surface tension allows better permeation and the evaporation rate is near sublimatic in process because of both the lower surface tension and higher vapor pressure [the lower surface tension maximizes the area and the thinness of the film, both of which increase evaporation flux]:
1. Bray, Comparison of Hexamethyldisilazane (HMDS), Peldri 11, and Critical-Point Drying Methods for SEM of Biological Specimens, Microsc Res Tech, 26, 489-495, 1993.
"Recently, several drying methods which claim to alleviate some of the disadvantages of CPD have been described. One of these methods (Kennedy et al., 1989) is similar to freeze drying and utilizes a sublimation dehydrant, Peldri 11, which is frozen and then sublimed from specimens. The Peldri 11 procedure requires no special equipment but can take several hours to perform a run. Other methods involve the use of low-surface- tension solvents such as Hexamethyldisilazane (HMDS) (Nation, 1983), tetramethylsilane (TMS) (Dey et al., 19891, and dimethoxypropane (DMP) (Weyda, 1992). These solvents evaporate directly from tissue in minutes, and infiltration times usually take { 1 hr."
2. Beno, Processing of soft pupae and uneclosed pharate adults of Drosophila for scanning electron microscopy, Microsc Res Tech, 70, 1022-1027, 2007
"We found that it is crucial to leave minute amount of above used solvents (ethanols, acetone, and mixture of acetone: hexamethyldisilazane) in eppendorf tube prior to each exchange to prevent animal from fast drying and damage."
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123 www.ph2llc.com (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, February 02, 2011 10:04 AM To: ph2-at-sprynet.com
Fascinating article. Some of my recollections (possibly with some inaccuracies but the best I can recollect) involve Fernandez-Moran who is credited with developing the first diamond knives for use with ultra-microtomes as well as a cryo ultra-microtome and a number of other interesting developments.
I worked for Fernandez-Moran at the U. of Chicago for a few years starting in 1963. This was after he was forced to leave Venezuela and then did a short stay at MIT before being recruited by U. of C. He had a microtome, presumably of his design, that only he used on rare occasion. Almost all the imaging done in the lab was with negatively stained samples.
He, of course, had his diamond knives. The technique to make them was perfected at the lab for neurological research that he built in Venezuela. (He was forced to leave Venezuela when the government was taken over by a military coup and he was on the wrong side, but that's another story.) He had a workshop there with his diamond cutters, etc. After developing the knives, he sent them out to the leading investigators of the day to get them to try them so that they would then buy them. Dupont picked up on the idea and also began making them for sale. Moran had patented the process so was able to sue Dupont and did win. I believe he later agreed to give them rights to make and market the knives.
Moran's lab at Chicago was quite a place. It was a semi-clean room lab in the basement of the Research Institute. The floors were raised so that all the water and vacuum lines for the microscopes were underneath with mechanical pumps and water recirculators a long ways away from the microscopes. He had 3 Seimens 1 and 1A TEMS on vibration mounts with the raised floor cut around the microscope bases so that moving a chair would not affect the TEM stability. A motor generator located in the attic of the building provided stable power. I started out as a technician and we all wore white nylon lab coats, white rubber shoes that we washed weekly, and little white hats. Visitors suited up in lab coats with plastic bags over their shoes. Pre-pumps to evacuate the film cameras were located two floors up. We would put on our red goggles to retain our dark adaptation, put plastic bags over our plastic shoes, and shuffle up to get new film cameras...looked like Martians!
It was an interesting time. There was also a couple of Japanese scientists working on an early Hitachi microscope trying to set resolution records. They would literally disassemble the microscope after just a few tries to clean it and get ready for the next attempts. They were the only ones with the patience to deal with that microscope. Later Perk and Elmer Company came along and helped with design changes to make it more user-friendly and thus more marketable.
Pointed filaments were also made in the lab. E.F. Fullam came one time to see how they were made and then was able to start selling them commercially.
Moran built a helium-cooled microscope to improve resolution using superconducting lenses. I do not know if it was originally his idea or if he "borrowed" someone else's idea. However, they built a liquid helium recirculator system, again with most of it in the attic 4 stories up. The helium flowed through a special jacket on the microscope that encased the entire upper part of the column through the objective lens. They managed to get a few images but then Moran lost interest. A series of health problems shortly thereafter led to closing down the lab and and end to a very interesting few years. Louie Ouwerkerk was a Dutch engineer who, along with the great U. of Chicago Instrument shop, designed and built a lot of Moran's ideas.
Debby
P.S. I have time to write this as the university is closed due to a major snow storm that hit us over the last 24 hours. Looks very pretty out there but....
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
On 2/2/11 6:43 AM, "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } Sorry for the delay in response to this topic, but due to an error on my on } my part my previous communication went astray. For those who may be } interested in the history of ultramicrotomy I do have a copy of an article } in PDF form which traces the development of the ultramicrotome from "wedge" } sections in the late thirties where the thin end of the wedge was } (hopefully) transparent to electrons, through the high speed era (actually } up to 57000 rpm), overcoming embedding limitations and finally discussing } the first generation of commercial instruments. } } Please contact me on terry.cooper-at-btinternet.com and I can attach the } missive, } } Best regards } } Terry Cooper } TAAB Laboratories Equipment } 3 Minerva House, Calleva Park } Aldermaston, Berks, RG7 8NA, England } Tel ++44(0)118 981 7775 } Fax ++44(0)118 981 7881 } e-mail sales-at-taab.co.uk } www.taab.co.uk } } } ==============================Original Headers============================== } 6, 39 -- From terry.cooper-at-btinternet.com Wed Feb 2 05:39:05 2011 } 6, 39 -- Received: from nm5.bullet.mail.ird.yahoo.com } (nm5.bullet.mail.ird.yahoo.com [77.238.189.62]) } 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p12Bd4GD004670 } 6, 39 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 05:39:04 -0600 } 6, 39 -- Received: from [77.238.189.57] by nm5.bullet.mail.ird.yahoo.com with } NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [212.82.108.246] by tm10.bullet.mail.ird.yahoo.com } with NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [127.0.0.1] by omp1011.mail.ird.yahoo.com with NNFMP; } 02 Feb 2011 11:39:03 -0000 } 6, 39 -- X-Yahoo-Newman-Id: 779398.69037.bm-at-omp1011.mail.ird.yahoo.com } 6, 39 -- Received: (qmail 86766 invoked from network); 2 Feb 2011 11:39:03 } -0000 } 6, 39 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 39 -- s=s1024; d=btinternet.com; } 6, 39 -- } h=DKIM-Signature:Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Mes } sage-ID:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encodi } ng:X-Priority:X-MSMail-Priority:X-Mailer:X-MimeOLE; } 6, 39 -- } b=IsObn+QLugwXVehYYiLw4s49xXwDqDZnhG9NNU4bIdzkofRt1VP0unu2tL6xZl2Sn4B0P6skvnc4 } oU3/Vz+MSfrTvXJJP0vwthYxKuegao2YJYJKxBY31StZ6EetQNgRNKsM9i+2WRIGannNqe0McTHQMO } CZ8dn6/wwzQ7mHXrA= ; } 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=btinternet.com; s=s1024; t=1296646743; } bh=oWdodXDAladTRTeAY1E2NNtKCYK+97yXwv8tXphMquI=; } h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:From:To } :Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X } -MSMail-Priority:X-Mailer:X-MimeOLE; } b=VKuj8pFNykoHwXnX54sk3fQwJkOZuoFXszGFPDSrEBKHHYq1DhiRlqIpTJ0MbhCZ90mrkMGJGx7j } vganojRhWDRuoc0kwwPzAnbIlk/bme1OYZj+omRhbm7WR6+jbygWxaJiNoCrMyI3euTEnUXnS9XDuE } ToKBTrHyxyDEMCDBc= } 6, 39 -- Received: from Pioneer (terry.cooper-at-94.30.34.8 with login) } 6, 39 -- by smtp817.mail.ird.yahoo.com with SMTP; 02 Feb 2011 11:39:00 } +0000 GMT } 6, 39 -- X-Yahoo-SMTP: } pVuM3eCswBBmTWesC4KmNptnh5To2FPTm29gm3HoxqLR7BH.yfiY1w-- } 6, 39 -- X-YMail-OSG: NozSGW4VM1m6bRR8Zaj1i1Ns2oY8lipJOBHfz_emqEZD7Mz } 6, 39 -- zVSXR5KJ0BzFhcVKMQO.WbUOmXArrNSCPPZWh8tM6axZRAE73cHQ.ln61SO9 } 6, 39 -- DQkj6etKzZY_ajLLLC.zUoMqYjs2wMLSAAl2QujHC5Kb13GH4ItZm4d3Q64m } 6, 39 -- J6zDCnwQouOZrxCbBg4v2t3bwm5d9oDgemTErzM7csvNyzX8QVdqPncU6CZ8 } 6, 39 -- qx4oeuCZHTwoR4VAvY0LcYBzAhjEUfvcns1bVlRfFM_eK5_EWeFSz6DHdKqE } 6, 39 -- lLKPz1rsDlBI- } 6, 39 -- X-Yahoo-Newman-Property: ymail-3 } 6, 39 -- Message-ID: {8F6E8C47CA0E40BB89DFF1D968EE4462-at-Pioneer} } 6, 39 -- From: "terry cooper" {terry.cooper-at-btinternet.com} } 6, 39 -- To: {microscopy-at-microscopy.com} } 6, 39 -- Subject: Ultramicrotome history } 6, 39 -- Date: Wed, 2 Feb 2011 11:39:22 -0000 } 6, 39 -- MIME-Version: 1.0 } 6, 39 -- Content-Type: text/plain; } 6, 39 -- format=flowed; } 6, 39 -- charset="iso-8859-1"; } 6, 39 -- reply-type=original } 6, 39 -- Content-Transfer-Encoding: 7bit } 6, 39 -- X-Priority: 3 } 6, 39 -- X-MSMail-Priority: Normal } 6, 39 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 6, 39 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 31 -- From dsherman-at-purdue.edu Wed Feb 2 10:37:11 2011 17, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 17, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12GbBEo011139 17, 31 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 10:37:11 -0600 17, 31 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) 17, 31 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p12Gb70v030385; 17, 31 -- Wed, 2 Feb 2011 11:37:07 -0500 17, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.190]) by WPPEXHUB03H.purdue.lcl 17, 31 -- ([172.21.6.92]) with mapi; Wed, 2 Feb 2011 11:37:07 -0500 17, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 17, 31 -- To: "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} , 17, 31 -- "message to: 17, 31 -- MSA list" {microscopy-at-microscopy.com} 17, 31 -- Date: Wed, 2 Feb 2011 11:37:05 -0500 17, 31 -- Subject: Re: [Microscopy] Ultramicrotome history 17, 31 -- Thread-Topic: [Microscopy] Ultramicrotome history 17, 31 -- Thread-Index: AcvCzlqhWM0c9QOSSAKdkNfd5A5brwAKRJGZ 17, 31 -- Message-ID: {C96EF261.B8FB%dsherman-at-purdue.edu} 17, 31 -- In-Reply-To: {201102021143.p12Bh3sW007617-at-ns.microscopy.com} 17, 31 -- Accept-Language: en-US 17, 31 -- Content-Language: en-US 17, 31 -- X-MS-Has-Attach: 17, 31 -- X-MS-TNEF-Correlator: 17, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 17, 31 -- acceptlanguage: en-US 17, 31 -- Content-Type: text/plain; charset="us-ascii" 17, 31 -- MIME-Version: 1.0 17, 31 -- X-PMX-Version: 5.5.9.388399 17, 31 -- X-PerlMx-Virus-Scanned: Yes 17, 31 -- Content-Transfer-Encoding: 8bit 17, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p12GbBEo011139 ==============================End of - Headers==============================
We recently acquired a vintage Reichert- Jung, Cryocut 1800. Unfortunately, it is missing much of the knife holder assemble. Does anyone on the list have these parts and are willing to sell or surplus them? The unit is in good condition and should serve us well.
Thanks so much.
Steve
-- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
==============================Original Headers============================== 6, 18 -- From steven.samuelsson-at-sri.com Wed Feb 2 11:16:37 2011 6, 18 -- Received: from mail1.sri.com (mail1.SRI.COM [128.18.30.17]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12HGbQq011642 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 11:16:37 -0600 6, 18 -- MIME-version: 1.0 6, 18 -- Content-transfer-encoding: 7BIT 6, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 6, 18 -- Received: from Steves-iMac.local ([unknown] [128.18.51.101]) 6, 18 -- by mail.sri.com (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built 6, 18 -- Jul 30 2009)) with ESMTP id {0LG0000CN2NOLRA0-at-mail.sri.com} for 6, 18 -- Microscopy-at-microscopy.com; Wed, 02 Feb 2011 09:16:36 -0800 (PST) 6, 18 -- Message-id: {4D499174.10708-at-sri.com} 6, 18 -- Date: Wed, 02 Feb 2011 09:16:36 -0800 6, 18 -- From: Steven Samuelsson {steven.samuelsson-at-sri.com} 6, 18 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.2.13) 6, 18 -- Gecko/20101207 Thunderbird/3.1.7 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: Cryostat Knife Holder Needed ==============================End of - Headers==============================
Great Story Debby, Thanks for sharing it with the list.
Stay Warm!
Best,
Neeraj.
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, February 02, 2011 11:46 AM To: Neeraj Gohad
Fascinating article. Some of my recollections (possibly with some inaccuracies but the best I can recollect) involve Fernandez-Moran who is credited with developing the first diamond knives for use with ultra-microtomes as well as a cryo ultra-microtome and a number of other interesting developments.
I worked for Fernandez-Moran at the U. of Chicago for a few years starting in 1963. This was after he was forced to leave Venezuela and then did a short stay at MIT before being recruited by U. of C. He had a microtome, presumably of his design, that only he used on rare occasion. Almost all the imaging done in the lab was with negatively stained samples.
He, of course, had his diamond knives. The technique to make them was perfected at the lab for neurological research that he built in Venezuela. (He was forced to leave Venezuela when the government was taken over by a military coup and he was on the wrong side, but that's another story.) He had a workshop there with his diamond cutters, etc. After developing the knives, he sent them out to the leading investigators of the day to get them to try them so that they would then buy them. Dupont picked up on the idea and also began making them for sale. Moran had patented the process so was able to sue Dupont and did win. I believe he later agreed to give them rights to make and market the knives.
Moran's lab at Chicago was quite a place. It was a semi-clean room lab in the basement of the Research Institute. The floors were raised so that all the water and vacuum lines for the microscopes were underneath with mechanical pumps and water recirculators a long ways away from the microscopes. He had 3 Seimens 1 and 1A TEMS on vibration mounts with the raised floor cut around the microscope bases so that moving a chair would not affect the TEM stability. A motor generator located in the attic of the building provided stable power. I started out as a technician and we all wore white nylon lab coats, white rubber shoes that we washed weekly, and little white hats. Visitors suited up in lab coats with plastic bags over their shoes. Pre-pumps to evacuate the film cameras were located two floors up. We would put on our red goggles to retain our dark adaptation, put plastic bags over our plastic shoes, and shuffle up to get new film cameras...looked like Martians!
It was an interesting time. There was also a couple of Japanese scientists working on an early Hitachi microscope trying to set resolution records. They would literally disassemble the microscope after just a few tries to clean it and get ready for the next attempts. They were the only ones with the patience to deal with that microscope. Later Perk and Elmer Company came along and helped with design changes to make it more user-friendly and thus more marketable.
Pointed filaments were also made in the lab. E.F. Fullam came one time to see how they were made and then was able to start selling them commercially.
Moran built a helium-cooled microscope to improve resolution using superconducting lenses. I do not know if it was originally his idea or if he "borrowed" someone else's idea. However, they built a liquid helium recirculator system, again with most of it in the attic 4 stories up. The helium flowed through a special jacket on the microscope that encased the entire upper part of the column through the objective lens. They managed to get a few images but then Moran lost interest. A series of health problems shortly thereafter led to closing down the lab and and end to a very interesting few years. Louie Ouwerkerk was a Dutch engineer who, along with the great U. of Chicago Instrument shop, designed and built a lot of Moran's ideas.
Debby
P.S. I have time to write this as the university is closed due to a major snow storm that hit us over the last 24 hours. Looks very pretty out there but....
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
On 2/2/11 6:43 AM, "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } Sorry for the delay in response to this topic, but due to an error on my on } my part my previous communication went astray. For those who may be } interested in the history of ultramicrotomy I do have a copy of an article } in PDF form which traces the development of the ultramicrotome from "wedge" } sections in the late thirties where the thin end of the wedge was } (hopefully) transparent to electrons, through the high speed era (actually } up to 57000 rpm), overcoming embedding limitations and finally discussing } the first generation of commercial instruments. } } Please contact me on terry.cooper-at-btinternet.com and I can attach the } missive, } } Best regards } } Terry Cooper } TAAB Laboratories Equipment } 3 Minerva House, Calleva Park } Aldermaston, Berks, RG7 8NA, England } Tel ++44(0)118 981 7775 } Fax ++44(0)118 981 7881 } e-mail sales-at-taab.co.uk } www.taab.co.uk } } } ==============================Original Headers============================== } 6, 39 -- From terry.cooper-at-btinternet.com Wed Feb 2 05:39:05 2011 } 6, 39 -- Received: from nm5.bullet.mail.ird.yahoo.com } (nm5.bullet.mail.ird.yahoo.com [77.238.189.62]) } 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p12Bd4GD004670 } 6, 39 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 05:39:04 -0600 } 6, 39 -- Received: from [77.238.189.57] by nm5.bullet.mail.ird.yahoo.com with } NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [212.82.108.246] by tm10.bullet.mail.ird.yahoo.com } with NNFMP; 02 Feb 2011 11:39:03 -0000 } 6, 39 -- Received: from [127.0.0.1] by omp1011.mail.ird.yahoo.com with NNFMP; } 02 Feb 2011 11:39:03 -0000 } 6, 39 -- X-Yahoo-Newman-Id: 779398.69037.bm-at-omp1011.mail.ird.yahoo.com } 6, 39 -- Received: (qmail 86766 invoked from network); 2 Feb 2011 11:39:03 } -0000 } 6, 39 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 39 -- s=s1024; d=btinternet.com; } 6, 39 -- } h=DKIM-Signature:Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Mes } sage-ID:From:To:Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encodi } ng:X-Priority:X-MSMail-Priority:X-Mailer:X-MimeOLE; } 6, 39 -- } b=IsObn+QLugwXVehYYiLw4s49xXwDqDZnhG9NNU4bIdzkofRt1VP0unu2tL6xZl2Sn4B0P6skvnc4 } oU3/Vz+MSfrTvXJJP0vwthYxKuegao2YJYJKxBY31StZ6EetQNgRNKsM9i+2WRIGannNqe0McTHQMO } CZ8dn6/wwzQ7mHXrA= ; } 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=btinternet.com; s=s1024; t=1296646743; } bh=oWdodXDAladTRTeAY1E2NNtKCYK+97yXwv8tXphMquI=; } h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:From:To } :Subject:Date:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Priority:X } -MSMail-Priority:X-Mailer:X-MimeOLE; } b=VKuj8pFNykoHwXnX54sk3fQwJkOZuoFXszGFPDSrEBKHHYq1DhiRlqIpTJ0MbhCZ90mrkMGJGx7j } vganojRhWDRuoc0kwwPzAnbIlk/bme1OYZj+omRhbm7WR6+jbygWxaJiNoCrMyI3euTEnUXnS9XDuE } ToKBTrHyxyDEMCDBc= } 6, 39 -- Received: from Pioneer (terry.cooper-at-94.30.34.8 with login) } 6, 39 -- by smtp817.mail.ird.yahoo.com with SMTP; 02 Feb 2011 11:39:00 } +0000 GMT } 6, 39 -- X-Yahoo-SMTP: } pVuM3eCswBBmTWesC4KmNptnh5To2FPTm29gm3HoxqLR7BH.yfiY1w-- } 6, 39 -- X-YMail-OSG: NozSGW4VM1m6bRR8Zaj1i1Ns2oY8lipJOBHfz_emqEZD7Mz } 6, 39 -- zVSXR5KJ0BzFhcVKMQO.WbUOmXArrNSCPPZWh8tM6axZRAE73cHQ.ln61SO9 } 6, 39 -- DQkj6etKzZY_ajLLLC.zUoMqYjs2wMLSAAl2QujHC5Kb13GH4ItZm4d3Q64m } 6, 39 -- J6zDCnwQouOZrxCbBg4v2t3bwm5d9oDgemTErzM7csvNyzX8QVdqPncU6CZ8 } 6, 39 -- qx4oeuCZHTwoR4VAvY0LcYBzAhjEUfvcns1bVlRfFM_eK5_EWeFSz6DHdKqE } 6, 39 -- lLKPz1rsDlBI- } 6, 39 -- X-Yahoo-Newman-Property: ymail-3 } 6, 39 -- Message-ID: {8F6E8C47CA0E40BB89DFF1D968EE4462-at-Pioneer} } 6, 39 -- From: "terry cooper" {terry.cooper-at-btinternet.com} } 6, 39 -- To: {microscopy-at-microscopy.com} } 6, 39 -- Subject: Ultramicrotome history } 6, 39 -- Date: Wed, 2 Feb 2011 11:39:22 -0000 } 6, 39 -- MIME-Version: 1.0 } 6, 39 -- Content-Type: text/plain; } 6, 39 -- format=flowed; } 6, 39 -- charset="iso-8859-1"; } 6, 39 -- reply-type=original } 6, 39 -- Content-Transfer-Encoding: 7bit } 6, 39 -- X-Priority: 3 } 6, 39 -- X-MSMail-Priority: Normal } 6, 39 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 } 6, 39 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 } ==============================End of - Headers==============================
==============================Original Headers============================== 17, 31 -- From dsherman-at-purdue.edu Wed Feb 2 10:37:11 2011 17, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 17, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12GbBEo011139 17, 31 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 10:37:11 -0600 17, 31 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) 17, 31 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p12Gb70v030385; 17, 31 -- Wed, 2 Feb 2011 11:37:07 -0500 17, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.190]) by WPPEXHUB03H.purdue.lcl 17, 31 -- ([172.21.6.92]) with mapi; Wed, 2 Feb 2011 11:37:07 -0500 17, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 17, 31 -- To: "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} , 17, 31 -- "message to: 17, 31 -- MSA list" {microscopy-at-microscopy.com} 17, 31 -- Date: Wed, 2 Feb 2011 11:37:05 -0500 17, 31 -- Subject: Re: [Microscopy] Ultramicrotome history 17, 31 -- Thread-Topic: [Microscopy] Ultramicrotome history 17, 31 -- Thread-Index: AcvCzlqhWM0c9QOSSAKdkNfd5A5brwAKRJGZ 17, 31 -- Message-ID: {C96EF261.B8FB%dsherman-at-purdue.edu} 17, 31 -- In-Reply-To: {201102021143.p12Bh3sW007617-at-ns.microscopy.com} 17, 31 -- Accept-Language: en-US 17, 31 -- Content-Language: en-US 17, 31 -- X-MS-Has-Attach: 17, 31 -- X-MS-TNEF-Correlator: 17, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 17, 31 -- acceptlanguage: en-US 17, 31 -- Content-Type: text/plain; charset="us-ascii" 17, 31 -- MIME-Version: 1.0 17, 31 -- X-PMX-Version: 5.5.9.388399 17, 31 -- X-PerlMx-Virus-Scanned: Yes 17, 31 -- Content-Transfer-Encoding: 8bit 17, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p12GbBEo011139 ==============================End of - Headers==============================
==============================Original Headers============================== 29, 37 -- From NEERAJG-at-clemson.edu Wed Feb 2 13:27:10 2011 29, 37 -- Received: from mailclient2.clemson.edu (mailclient2.clemson.edu [130.127.237.181]) 29, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12JRADa030965 29, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 13:27:10 -0600 29, 37 -- Received: from mailclient2.clemson.edu (localhost.clemson.edu [127.0.0.1]) 29, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p12JR9Qq003596 29, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 14:27:09 -0500 29, 37 -- Received: from frontex-2.CAMPUS.CU.CLEMSON.EDU (frontex-2.clemson.edu [130.127.235.60]) 29, 37 -- by mailclient2.clemson.edu (8.13.8/8.13.8) with ESMTP id p12JR6tl003522 29, 37 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 29, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 14:27:09 -0500 29, 37 -- Received: from exch07.CAMPUS.CU.CLEMSON.EDU ([fe80::51d9:89cd:bf90:3ce4]) by 29, 37 -- frontex-2.CAMPUS.CU.CLEMSON.EDU ([2002:827f:eb3c::827f:eb3c]) with mapi; Wed, 29, 37 -- 2 Feb 2011 14:27:04 -0500 29, 37 -- From: Neeraj Gohad {NEERAJG-at-clemson.edu} 29, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 29, 37 -- Date: Wed, 2 Feb 2011 14:27:05 -0500 29, 37 -- Subject: RE: [Microscopy] Re: Ultramicrotome history 29, 37 -- Thread-Topic: [Microscopy] Re: Ultramicrotome history 29, 37 -- Thread-Index: AcvC+KkIP1wqPY1YQUiO6yij2tOUkAAFkaXQ 29, 37 -- Message-ID: {026D23F784693D468AA3DABB5A2D628F993874E237-at-EXCH07.CAMPUS.CU.CLEMSON.EDU} 29, 37 -- References: {201102021645.p12GjloQ027029-at-ns.microscopy.com} 29, 37 -- In-Reply-To: {201102021645.p12GjloQ027029-at-ns.microscopy.com} 29, 37 -- Accept-Language: en-US 29, 37 -- Content-Language: en-US 29, 37 -- X-MS-Has-Attach: 29, 37 -- X-MS-TNEF-Correlator: 29, 37 -- acceptlanguage: en-US 29, 37 -- Content-Type: text/plain; charset="us-ascii" 29, 37 -- MIME-Version: 1.0 29, 37 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 29, 37 -- definitions=2011-02-02_07:2011-02-02,2011-02-02,1970-01-01 signatures=0 29, 37 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=8 29, 37 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 29, 37 -- engine=5.0.0-1012030000 definitions=main-1102020143 29, 37 -- Content-Transfer-Encoding: 8bit 29, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p12JRADa030965 ==============================End of - Headers==============================
Store for how long? What kind of specimens? I have stored soft tissue samples for a couple of days in glut with no problems, and crustaceans with well sclerotized cuticles for several days, but ... lightly sclerotized crusties and body parts don't like it. (Note! Watch the pH! The fix can cause dissolution of Ca in the crustacean exoskeleton.) When I had to process, store, then transport samples from Antarctica to Chicago, I ran them up into 100% Pel-Dri (back when it was allowed) or 100% HMDS. This worked. The samples fish lateral-lines and lateral-line end organs, and they compared well to freshly fixed, processed, and dried end-organs collected in Lake Michigan. The sample vials where completely full of fluid, with a 10:1 or 20:1 fluid:specimen volume ratio. But. The best way is to fix, process, dry, mount, and stick in a desiccator. Don't coat. The expansion and shrinkage of the samples when moving them from the desiccator to the SEM and back can cause fine cracks in a sputter-coated metal layer. Coat when ready to examine.
Phil
} Email: wadowska-at-upei.ca Name: Dorota Wadowska } } Organization: Atlantic Veterinary College at UPEI } } Title-Subject: [Filtered] SEM sample storing } } Message: Hello all, } One of our EM lab users asked me a question: what is the best way to } store SEM samples? I know you'll all say that the best is to process and } coat first. He wants to store them in 2% glutaraldehyde and process } later since he does not have time at the moment. What is your } experience? I am not an expert in SEM. } Thank you Dorota } Login Host: 137.149.102.148
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 26 -- From oshel1pe-at-cmich.edu Thu Feb 3 08:43:34 2011 4, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p13EhYSY020694 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Feb 2011 08:43:34 -0600 4, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p13EhDfO006000; 4, 26 -- Thu, 3 Feb 2011 09:43:33 -0500 4, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 4, 26 -- Thu, 3 Feb 2011 09:43:19 -0500 4, 26 -- Mime-Version: 1.0 4, 26 -- Message-Id: {a06240806c9706cadd430-at-[141.209.160.249]} 4, 26 -- In-Reply-To: {201102021401.p12E1uLR030298-at-ns.microscopy.com} 4, 26 -- References: {201102021401.p12E1uLR030298-at-ns.microscopy.com} 4, 26 -- Date: Thu, 3 Feb 2011 09:43:18 -0500 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 26 -- Subject: Re: [Microscopy] viaWWW:SEM sample storing 4, 26 -- Cc: wadowska-at-upei.ca 4, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 4, 26 -- X-OriginalArrivalTime: 03 Feb 2011 14:43:19.0702 (UTC) FILETIME=[B31F3B60:01CBC3B0] 4, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 26 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 4, 26 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 26 -- X-CanItPRO-Stream: default 4, 26 -- X-Canit-Stats-ID: 02E3qHx1j - a18eee929027 - 20110203 4, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-lboro.ac.uk Name: Zhou Organization: Dept of Materials, Loughborough University, UK
Title-Subject: [Filtered] Silicone rubber Ultramicrotome for TEM
Message: I use a Cambridge Huxley ultra-microtome to slice polymer composites for TEM. The samples, such as clays in Polypropylene, nanotubes in epoxy, are embedded in an Agar low viscosity resin (set at 60 degreeC). I operate it at room temperature, and cut the above polymers fine. Recently, there are some really soft polymers, such as organoclay filled silicone rubber, polyethylene, etc.., which I found difficult to get flat slices.
I was suggested to try low temperature; say -70degree C. But the lab does not have a cryo-microtome. We thought about first dipping the embedded sample in liquid N2, then slicing it as usual at room temperature, as long as the temperature donÂ’t affect the samples. My question is---- can my diamond knife (Drukker international) survive that low temperature? Or a glass knife? Has anybody tried this before? Any tricks or experience would be very much appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lysa.chizmadia-at-upr.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I hope you had a happy holiday season. We have finally solved our bad water chiller situation and I'm ready to fire up our SX50 for the first time in two years. However, I'm concerned about any maintenance I should perform on the vacuum pumps since they haven't run in quite some time. Thank you very much.
Drawing from my cleanroom experience.... HMDS is used as a photoresist adhesion agent for Si wafers. It binds to hydroxyl groups. It makes sense to think of as having mordant properties, strengthening the specimen with Si. The low surface tension also plays an important role.
http://www.transene.com/hexa.html
cheers ed
Edward J. Basgall, Ph.D. Microscopy Manager Drexel University - Central Research Facility 106 Bossone Research Center 31st and Market Streets Philadelphia, PA 19104 office: (215) 895-2379
On Wed, Feb 2, 2011 at 10:07 AM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both bbandli-at-d.umn.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: bbandli-at-d.umn.edu Name: Bryan Bandli } } Organization: University of Minnesota, Duluth } } Title-Subject: [Filtered] HMDS, How does it work... } } Message: Hi All, } } I'm looking for a reference to explain just how/why HMDS works to dry } specimens for SEM observation. There are plenty of references showing } that it does, in fact, work and produces good results in many cases but } I have yet to find an explanation as to the "why". } Is it that HMDS has the right physical properties (surface tension) to } evaporate without causing drying artifacts? } } Or, is there something about the silanization of the sample that helps } make it more robust, or both, or am I completely lost (quite likely)? } } Thanks in advance for your input! } } Cheers, } } Bryan Bandli } University of MN, Duluth } } Login Host: 131.212.37.204 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 2 08:59:53 2011 } 13, 26 -- Received: from hiltoncluster2.worldspice.net (hiltoncluster2.worldspice.net [216.37.94.62]) } 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p12Exrm0009047 } 13, 26 -- for {microscopy-at-microscopy.com} ; Wed, 2 Feb 2011 08:59:53 -0600 } 13, 26 -- Received: (qmail 10492 invoked by uid 0); 2 Feb 2011 14:59:50 -0000 } 13, 26 -- Received: by simscan 1.4.0 ppid: 10372, pid: 10454, t: 1.5267s } 13, 26 -- scanners: clamav: 0.95.3/m:51/d:10354 spam: 3.3.0 } 13, 26 -- X-Spam-Checker-Version: SpamAssassin 3.3.1-wspice_748 (2010-03-16) on } 13, 26 -- hiltoncluster2.hiltonsmtp.worldspice.net } 13, 26 -- X-Spam-Level: **** } 13, 26 -- X-Spam-Status: No, score=4.3 Bayes=0.5 required=6.5 autolearn=disabled version=3.3.1-wspice_748 report= } 13, 26 -- * 2.0 CS_290 BODY: CS_290 } 13, 26 -- * 0.9 CS_796 CS_796 } 13, 26 -- * 1.4 TW_HM BODY: Odd Letter Triples with HM } 13, 26 -- Received: from unknown (HELO NestorPwrBk.local) (zaluzec-at-12.97.46.2) } 13, 26 -- by hiltoncluster02.worldspice.net with ESMTPA; 2 Feb 2011 14:59:49 -0000 } 13, 26 -- Message-ID: {4D49715A.5000308-at-microscopy.com} } 13, 26 -- Date: Wed, 02 Feb 2011 06:59:38 -0800 } 13, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 13, 26 -- Reply-To: bbandli-at-d.umn.edu } 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 26 -- MIME-Version: 1.0 } 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 26 -- Subject: viaWWW:HMDS, How does it work... } 13, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 26 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Edward Basgall 103 N. Drexel Ave. Havertown, PA 19083
c: 610-574-4007
==============================Original Headers============================== 13, 35 -- From ejb1176-at-gmail.com Thu Feb 3 23:32:16 2011 13, 35 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) 13, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p145WGhX027742 13, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Feb 2011 23:32:16 -0600 13, 35 -- Received: by wyj26 with SMTP id 26so1981554wyj.0 13, 35 -- for {Microscopy-at-microscopy.com} ; Thu, 03 Feb 2011 21:32:15 -0800 (PST) 13, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 35 -- d=gmail.com; s=gamma; 13, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 13, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 13, 35 -- bh=qovlBtp/UFsmdSMP0XANOdEFSA/AIHBmlln3ya/i4wc=; 13, 35 -- b=LijH0vuRLJHOLDx4VzWHjqNskuN9eufeDnjHaI8k4YoHvT30d3QloW43WG678e9hVb 13, 35 -- 3zU06knMfL0HGw8FAqvaNJRJ7AvelxPkjAmzIRzDnPxLfLz0k0aOzISydaNRKqrAqEoF 13, 35 -- hl8aNZIzwTm6Wr2KiYm647+eBhcK6wXVu9bu8= 13, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 13, 35 -- d=gmail.com; s=gamma; 13, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 13, 35 -- :content-type:content-transfer-encoding; 13, 35 -- b=Jp1l3S4C4PJBeXXj8aMIItJ94mt2S5ygZ7Tr9iNDW2tAU9ZI2KRHmqh0AwE8FJT8kp 13, 35 -- Ypxrk5gq+5zp7BxMa76cZTgqYdQCf66yxtbx1T8laAZE9epaHk5oiG3pmng12x0/xaQP 13, 35 -- AqMMp4vdM0+k+2LN1XxcehzkT457r43+bkz7w= 13, 35 -- MIME-Version: 1.0 13, 35 -- Received: by 10.216.71.13 with SMTP id q13mr10707721wed.21.1296797535211; Thu, 13, 35 -- 03 Feb 2011 21:32:15 -0800 (PST) 13, 35 -- Received: by 10.216.24.206 with HTTP; Thu, 3 Feb 2011 21:32:15 -0800 (PST) 13, 35 -- In-Reply-To: {201102021507.p12F7tC2021307-at-ns.microscopy.com} 13, 35 -- References: {201102021507.p12F7tC2021307-at-ns.microscopy.com} 13, 35 -- Date: Fri, 4 Feb 2011 00:32:15 -0500 13, 35 -- Message-ID: {AANLkTimn+w7u9_GxoBCv-jd28mWuSiZOkKpr=Y+t-ann-at-mail.gmail.com} 13, 35 -- Subject: Re: [Microscopy] viaWWW:HMDS, How does it work... 13, 35 -- From: EJB {ejb1176-at-gmail.com} 13, 35 -- To: bbandli-at-d.umn.edu, MSA_listserve {Microscopy-at-microscopy.com} 13, 35 -- Content-Type: text/plain; charset=ISO-8859-1 13, 35 -- Content-Transfer-Encoding: 8bit 13, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p145WGhX027742 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rick.hughes-at-microanalysis.com.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rick.hughes-at-microanalysis.com.au Name: Rick Hughes
Organization: Microanalysis Australia
Title-Subject: [Filtered] PC interfaced Penning/cold cathode options?
Message: Does anyone have any recommendations for a PC interfaced Penning/cold cathode vacuum gauge? We do a lot of web interfaced SEM control and it would be an added bonus to be able to check the chamber vacuum when not sitting in front of the microscope!
Hi all, I need a twain driver for an old JVC TK-C1381 (same as 1380) CCD camera used for LM-work. Would really appreciate if someone could help me as I've searched the internet without any success.
Cheers,
Per Hörstedt EM Platform KBC Building University of Umea S-90186 Umea Sweden
==============================Original Headers============================== 7, 15 -- From per.horstedt-at-medbio.umu.se Mon Feb 7 04:27:37 2011 7, 15 -- Received: from mail.ad.umu.se (umdac-ch1.ad.umu.se [130.239.1.246]) 7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p17ARaoO008866 7, 15 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Feb 2011 04:27:37 -0600 7, 15 -- Received: from [130.239.34.7] (130.239.34.7) by mail.ad.umu.se (130.239.1.250) 7, 15 -- with Microsoft SMTP Server (TLS) id 8.1.436.0; Mon, 7 Feb 2011 11:27:11 +0100 7, 15 -- Message-ID: {4D4FC8FE.2010308-at-medbio.umu.se} 7, 15 -- Date: Mon, 7 Feb 2011 11:27:10 +0100 7, 15 -- From: =?ISO-8859-1?Q?per_h=F6rstedt?= {per.horstedt-at-medbio.umu.se} 7, 15 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 15 -- MIME-Version: 1.0 7, 15 -- To: {Microscopy-at-microscopy.com} 7, 15 -- Subject: ccd camera driver 7, 15 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 7, 15 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ianlamswood44-at-yahoo.co.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ianlamswood44-at-yahoo.co.uk Name: Ian Lamswood
Organization: Leica Microsystems
Title-Subject: [Filtered] Product Manager
Message: Job Opportunity - Product Manager.
Leica Microsystems is seeking applicants for a Product Manager in the area of EM Sample Preparation. Based in the Nanotechnology Business Unit, Vienna, Austria, the potential candidate will be responsible for instruments in the area of sample coating and freeze fracture.
A good knowledge of sample preparation for biological and/or industrial materials samples is necessary and knowledge of freeze fracture and coating a pre-requisite. Sales and product management skills would be an asset. A good knowledge of the English language is required and German is also useful. An attractive remuneration package is available to the successful candidate.
For further details please email your CV to: Thomas Remes, HR Manager, Leica Microsystems, Vienna, Austria
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both subbakaravadi-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Interfacing problem on FEI TIA/Emispec ESVision and Gatan Digital Micrograph for EELS
Message: Problems interfacing FEI TIA/Emispec ESVision and Gatan Digital Micrograph for EELS
We are experiencing a problem in accessing EELS spectrum through TIA/ESVision after we upgraded our computer operating systems to Windows XP and Digital micrograph from 1.6 to 1.8. The error message while opening ES vision is
“Cannot load plugin for GatanCCD.dll”
We have not made any changes to ES vision or the Emispec computer. The two computers are connected through an Ethernet cable. I would greatly appreciate your suggestion on this issue.
We were unable to get Emispec to work on Windows XP. We didn't try to upgrade to DM 1.8, but we did have camera driver problems.
I suggest you look for funding to replace the Emispec with Gatan Digiscan II -- it has all the same capabilities and more, and it supported by a manufacturer. We replaced Emispec with Digiscan II on our CM200 FEG and it works like a champ, providing TEM, EFTEM, EDS, and EELS in an integrated package.
Chad Parish
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Monday, February 07, 2011 6:26 PM To: Parish, Chad M.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both subbakaravadi-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Interfacing problem on FEI TIA/Emispec ESVision and Gatan Digital Micrograph for EELS
Message: Problems interfacing FEI TIA/Emispec ESVision and Gatan Digital Micrograph for EELS
We are experiencing a problem in accessing EELS spectrum through TIA/ESVision after we upgraded our computer operating systems to Windows XP and Digital micrograph from 1.6 to 1.8. The error message while opening ES vision is
Â"Cannot load plugin for GatanCCD.dllÂ"
We have not made any changes to ES vision or the Emispec computer. The two computers are connected through an Ethernet cable. I would greatly appreciate your suggestion on this issue.
We need to section clay soil samples for STXM-NEXAFS analysis to a thickness of about 0.5 micron. The problem is that some of the soils contain glass particles so we have to use a glass knife to get sections. The second problem is the researchers want to know if carbon is coating the soil particles, so we can't embed them in any type of resin. I attempted to attach the soil to a BEEM capsule with superglue and then section the particles sticking off the surface, but the researchers suspect that superglue got on/in their particles. Moreover, the soil particles more or less fractured during sectioning at 0.5 micron thickness rather than being uniformly cut.
Does anyone have any experience dealing with this type of sample? My background is with TEM of soft biological samples, so any advice or suggestions would be greatly appreciated.
Thanks,
Shannon
Delaware Biotechnology Institute 15 Innovation Way Newark, DE 19711
==============================Original Headers============================== 6, 26 -- From modla-at-dbi.udel.edu Tue Feb 8 07:49:03 2011 6, 26 -- Received: from mail.dbi.udel.edu (genome3.dbi.udel.edu [128.175.253.195]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p18Dn37u028366 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 07:49:03 -0600 6, 26 -- Received: from localhost (localhost [127.0.0.1]) 6, 26 -- by mail.dbi.udel.edu (Postfix) with ESMTP id BA47E7C009 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 08:49:02 -0500 (EST) 6, 26 -- X-Virus-Scanned: amavisd-new at dbi.udel.edu 6, 26 -- Received: from mail.dbi.udel.edu ([127.0.0.1]) 6, 26 -- by localhost (mail.dbi.udel.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id XVr0xS-hX39q for {Microscopy-at-microscopy.com} ; 6, 26 -- Tue, 8 Feb 2011 08:48:59 -0500 (EST) 6, 26 -- Received: from [128.175.253.84] (host-253-84.nss.udel.edu [128.175.253.84]) 6, 26 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 6, 26 -- (No client certificate requested) 6, 26 -- by mail.dbi.udel.edu (Postfix) with ESMTPS id D9E407C008 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 08:48:59 -0500 (EST) 6, 26 -- Message-ID: {4D5149DB.10902-at-dbi.udel.edu} 6, 26 -- Date: Tue, 08 Feb 2011 08:49:15 -0500 6, 26 -- From: Shannon Modla {modla-at-dbi.udel.edu} 6, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 6, 26 -- MIME-Version: 1.0 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Subject: Help with Sectioning Soil 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
No idea really, but how about if the soil is hydrated, freezing and then microtoming
or freezing it, putting in a cryostage and making a section with FIB, if that's possible (does anyone do CryoFIB????)
Anyway, in either case, if you can't use resin, then hold it together with frozen water.
Is this any use or are my ideas completely off-base?
Ian
} Hi Everyone, } } We need to section clay soil samples for STXM-NEXAFS analysis to a } thickness of about 0.5 micron. The problem is that some of the soils } contain glass particles so we have to use a glass knife to get } sections. The second problem is the researchers want to know if carbon } is coating the soil particles, so we can't embed them in any type of } resin. I attempted to attach the soil to a BEEM capsule with superglue } and then section the particles sticking off the surface, but the } researchers suspect that superglue got on/in their particles. Moreover, } the soil particles more or less fractured during sectioning at 0.5 } micron thickness rather than being uniformly cut. } } Does anyone have any experience dealing with this type of sample? My } background is with TEM of soft biological samples, so any advice or } suggestions would be greatly appreciated. } } Thanks, } } Shannon
Dr Ian MacLaren BSc (Hons), PhD, MInstP, CPhys Lecturer in Physics
Dear Rao, We use ES Vision 4 on an XP computer along with Gatan Digital Micrograph v1.8 (all in one computer). The problem you are having refers to a plugin file that should be in the EmiSpec/ES Vision/Plugin Files folder. This should be reloaded if it is not there. If you have lost it contact me offline and I should be able to get that file to you. The only problem with Emispec software is that there are very few people left that know anything about it. It was the precursor for the FEI TIA platform, but is now so many versions behind that it is a different species now! Ken
On Feb 7, 2011, at 6:13 PM, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both subbakaravadi-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: subbakaravadi-at-gmail.com Name: Rao Karavadi } } Organization: Penn State University } } Title-Subject: [Filtered] Interfacing problem on FEI TIA/Emispec } ESVision and Gatan Digital Micrograph for EELS } } Message: Problems interfacing FEI TIA/Emispec ESVision and Gatan Digital } Micrograph for EELS } } We are experiencing a problem in accessing EELS spectrum through } TIA/ESVision after we upgraded our computer operating systems to Windows } XP and Digital micrograph from 1.6 to 1.8. The error message while } opening ES vision is } } “Cannot load plugin for GatanCCD.dll” } } We have not made any changes to ES vision or the Emispec computer. The } two computers are connected through an Ethernet cable. } I would greatly appreciate your suggestion on this issue. } } Thanks } Rao } } } } } Login Host: 24.228.81.124 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 26 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 7 17:07:54 2011 } 15, 26 -- Received: from znl.com ([206.69.208.20]) } 15, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p17N7sRX031115 } 15, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Feb 2011 17:07:54 -0600 } 15, 26 -- Received: from localhost (localhost [127.0.0.1]) } 15, 26 -- by znl.com (Postfix) with ESMTP id 688AE32A6A4 } 15, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Feb 2011 17:07:54 -0600 (CST) } 15, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 15, 26 -- Received: from znl.com ([127.0.0.1]) } 15, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 15, 26 -- with ESMTP id hrvsqmaTNmvW for {microscopy-at-microscopy.com} ; } 15, 26 -- Mon, 7 Feb 2011 17:07:53 -0600 (CST) } 15, 26 -- Received: from NestorPwrBk.local (nsit-dhcp-205-208-121-104.uchicago.edu [205.208.121.104]) } 15, 26 -- by znl.com (Postfix) with ESMTPA id 0C23F32A699 } 15, 26 -- for {microscopy-at-microscopy.com} ; Mon, 7 Feb 2011 17:07:51 -0600 (CST) } 15, 26 -- Message-ID: {4D507B45.5050702-at-microscopy.com} } 15, 26 -- Date: Tue, 08 Feb 2011 10:07:49 +1100 } 15, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 15, 26 -- Reply-To: subbakaravadi-at-gmail.com } 15, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 15, 26 -- MIME-Version: 1.0 } 15, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 26 -- Subject: viaWWW:Interfacing problem on FEI TIA/Emispec ESVision and Gatan } 15, 26 -- Digital Micrograph for EELS } 15, 26 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 15, 26 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
|_|"|_|"|_|_|"|_|"|_|"|_|_|"|_|"|_|_|"|_|"|_|"|_|_| Kenneth JT Livi, PhD Director, The High-Resolution Analytical Electron Microbeam Facility of the Integrated Imaging Center Departments of Earth and Planetary Sciences and Biology Olin Hall 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA | | | .| | .| | .| | .| | | :| | | .| | .| | .| | .| | | :|
==============================Original Headers============================== 13, 21 -- From prvs=013206355=klivi-at-jhu.edu Tue Feb 8 12:11:17 2011 13, 21 -- Received: from ipex2.johnshopkins.edu (ipex2.johnshopkins.edu [162.129.8.151]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p18IBHrX002774 13, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 12:11:17 -0600 13, 21 -- X-IronPort-AV: E=Sophos;i="4.60,442,1291611600"; 13, 21 -- d="scan'208";a="46113299" 13, 21 -- Received: from olin60-128-220-60-145.olin.jhu.edu ([128.220.60.145]) 13, 21 -- by ipex2.johnshopkins.edu with ESMTP/TLS/AES128-SHA; 08 Feb 2011 13:11:00 -0500 13, 21 -- Subject: Re: [Microscopy] viaWWW:Interfacing problem on FEI TIA/Emispec ESVision and Gatan 13, 21 -- Mime-Version: 1.0 (Apple Message framework v1082) 13, 21 -- Content-Type: text/plain; charset=iso-8859-1 13, 21 -- From: Ken Livi {klivi-at-jhu.edu} 13, 21 -- In-Reply-To: {201102072313.p17NDuLx003061-at-ns.microscopy.com} 13, 21 -- Date: Tue, 8 Feb 2011 13:11:00 -0500 13, 21 -- Cc: Ken Livi {klivi-at-jhu.edu} 13, 21 -- Message-Id: {B6C51076-62B8-425B-8E3B-5B1B2534FCE7-at-jhu.edu} 13, 21 -- References: {201102072313.p17NDuLx003061-at-ns.microscopy.com} 13, 21 -- To: Microscopy-at-microscopy.com, subbakaravadi-at-gmail.com 13, 21 -- X-Mailer: Apple Mail (2.1082) 13, 21 -- Content-Transfer-Encoding: 8bit 13, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p18IBHrX002774 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tj_yen-at-fccc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Microscope Facility manager at the Fox Chase Cancer Center, Philadelphia, PA.
We are seeking a Ph.D. level Manager to oversee our Cell Imaging Facility. Interested individuals should have expertise in EM and high resolution quantitative light microscopy (confocal, time-lapse, and immunocytochemistry). Candidates familiar with immunogold labeling, and advanced imaging techniques such as FRET and FRAP are preferred. The cell imaging facility serves a diverse base of users with variable experience in advanced microscopy techniques. The duties of the manager include developing and incorporating new technologies into the facility, training users in the proper use of various imaging equipment, and assisting the Director in submitting instrumentation grant applications. This is a non-tenure track faculty position and the appointment is commensurate with experience. Applicants should submit their CV and statement of research interests along with references to: Roseanne.Brooks-at-fccc.edu
Our facility is currently using a Calcium calendar package (specifically Calcium 3.10.1 Professional 25 w/Email by Brown Bear Software) for online equipment scheduling. We're fairly pleased with it, but we've been notified of incompatibilities with upcoming changes to our website (programming and design, hosting, servers, etc). Unfortunately, I don't know the details at this time.
We're looking for something that's relatively inexpensive, secure, user-friendly, somewhat customizable, and allows us to send email notifications and export events to data files (which we can then import into Excel for recordkeeping, billing and analysis). And we need the option to administer calendars for at least ten different pieces of equipment.
We'd appreciate all the suggestions we can get. We'll take a look at them and run our selections past our webkeepers. (You may send responses directly to me offline.)
Thank you so much for your help,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 Saint Louis, MO 63110
Email: lettj-at-ent.wustl.edu Office: 314-747-7257 Fax: 314-747-7230 Website: http://otocore.wustl.edu This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail.
==============================Original Headers============================== 8, 23 -- From LettJ-at-ent.wustl.edu Tue Feb 8 14:35:42 2011 8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu [128.252.17.171]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p18KZgfP005572 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 14:35:42 -0600 8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.4675); 8, 23 -- Tue, 8 Feb 2011 14:35:41 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: FACILITIES online scheduling 8, 23 -- Date: Tue, 8 Feb 2011 14:35:42 -0600 8, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C3901E99687-at-EX04.wusm-pcf.wustl.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: FACILITIES online scheduling 8, 23 -- Thread-Index: AcvHz76b3PvcRBsUQtevV/+aRWBYQA== 8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 08 Feb 2011 20:35:41.0708 (UTC) FILETIME=[C0CE14C0:01CBC7CF] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p18KZgfP005572 ==============================End of - Headers==============================
Electron Micrographs of Clay Minerals Edited by: Toshio Sudo, Susumu Shimoda, Haruo Yotsumoto and Saburo Aita ISBN: 9780444997517
Promise of methods for TEM???
Best I can do. ________________________________________ X-from: ian.maclaren-at-glasgow.ac.uk [ian.maclaren-at-glasgow.ac.uk] Sent: Tuesday, February 08, 2011 9:13 AM To: Monson, Frederick
No idea really, but how about if the soil is hydrated, freezing and then microtoming
or freezing it, putting in a cryostage and making a section with FIB, if that's possible (does anyone do CryoFIB????)
Anyway, in either case, if you can't use resin, then hold it together with frozen water.
Is this any use or are my ideas completely off-base?
Ian
} Hi Everyone, } } We need to section clay soil samples for STXM-NEXAFS analysis to a } thickness of about 0.5 micron. The problem is that some of the soils } contain glass particles so we have to use a glass knife to get } sections. The second problem is the researchers want to know if carbon } is coating the soil particles, so we can't embed them in any type of } resin. I attempted to attach the soil to a BEEM capsule with superglue } and then section the particles sticking off the surface, but the } researchers suspect that superglue got on/in their particles. Moreover, } the soil particles more or less fractured during sectioning at 0.5 } micron thickness rather than being uniformly cut. } } Does anyone have any experience dealing with this type of sample? My } background is with TEM of soft biological samples, so any advice or } suggestions would be greatly appreciated. } } Thanks, } } Shannon
Dr Ian MacLaren BSc (Hons), PhD, MInstP, CPhys Lecturer in Physics
I am not a specialist of mother earth but I cannot imagine any kind of soil sample without superficial carbon contamination. Now here is my opinion: in your description your 2 problems can be solved separately. The first one is to verify if there is carbon contamination on the surface of your sample. In this case you don't need to cut sections, just put your sample in a SEM with a low kV and see if you can detect carbon. There may be other solutions, but in any case this does not require cutting sections.
Then you need to analyse sections. Now you have analyzed the surface you don't care if you add more carbon, just embed the best way possible, cut your sample and analyse the sections.
Alternatively, as already stated, you may try to etch the sample and analyse the etched surface with FIB (even without freezing).
Good luck,
Stephane
----- Original Message ---- X-from: "modla-at-dbi.udel.edu" {modla-at-dbi.udel.edu} To: nizets2-at-yahoo.com Sent: Tue, February 8, 2011 2:53:19 PM
Hi Everyone,
We need to section clay soil samples for STXM-NEXAFS analysis to a thickness of about 0.5 micron. The problem is that some of the soils contain glass particles so we have to use a glass knife to get sections. The second problem is the researchers want to know if carbon is coating the soil particles, so we can't embed them in any type of resin. I attempted to attach the soil to a BEEM capsule with superglue and then section the particles sticking off the surface, but the researchers suspect that superglue got on/in their particles. Moreover, the soil particles more or less fractured during sectioning at 0.5 micron thickness rather than being uniformly cut.
Does anyone have any experience dealing with this type of sample? My background is with TEM of soft biological samples, so any advice or suggestions would be greatly appreciated.
Thanks,
Shannon
Delaware Biotechnology Institute 15 Innovation Way Newark, DE 19711
==============================Original Headers============================== 6, 26 -- From modla-at-dbi.udel.edu Tue Feb 8 07:49:03 2011 6, 26 -- Received: from mail.dbi.udel.edu (genome3.dbi.udel.edu [128.175.253.195]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p18Dn37u028366 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 07:49:03 -0600 6, 26 -- Received: from localhost (localhost [127.0.0.1]) 6, 26 -- by mail.dbi.udel.edu (Postfix) with ESMTP id BA47E7C009 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 08:49:02 -0500 (EST) 6, 26 -- X-Virus-Scanned: amavisd-new at dbi.udel.edu 6, 26 -- Received: from mail.dbi.udel.edu ([127.0.0.1]) 6, 26 -- by localhost (mail.dbi.udel.edu [127.0.0.1]) (amavisd-new, port 10024) 6, 26 -- with ESMTP id XVr0xS-hX39q for {Microscopy-at-microscopy.com} ; 6, 26 -- Tue, 8 Feb 2011 08:48:59 -0500 (EST) 6, 26 -- Received: from [128.175.253.84] (host-253-84.nss.udel.edu [128.175.253.84]) 6, 26 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 6, 26 -- (No client certificate requested) 6, 26 -- by mail.dbi.udel.edu (Postfix) with ESMTPS id D9E407C008 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 08:48:59 -0500 (EST) 6, 26 -- Message-ID: {4D5149DB.10902-at-dbi.udel.edu} 6, 26 -- Date: Tue, 08 Feb 2011 08:49:15 -0500 6, 26 -- From: Shannon Modla {modla-at-dbi.udel.edu} 6, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 6, 26 -- MIME-Version: 1.0 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Subject: Help with Sectioning Soil 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
____________________________________________________________________________________ Sucker-punch spam with award-winning protection. Try the free Yahoo! Mail Beta. http://advision.webevents.yahoo.com/mailbeta/features_spam.html
==============================Original Headers============================== 23, 37 -- From nizets2-at-yahoo.com Wed Feb 9 02:32:40 2011 23, 37 -- Received: from nm30.bullet.mail.ne1.yahoo.com (nm30.bullet.mail.ne1.yahoo.com [98.138.90.93]) 23, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p198WdYp017228 23, 37 -- for {microscopy-at-microscopy.com} ; Wed, 9 Feb 2011 02:32:39 -0600 23, 37 -- Received: from [98.138.90.55] by nm30.bullet.mail.ne1.yahoo.com with NNFMP; 09 Feb 2011 08:32:39 -0000 23, 37 -- Received: from [98.138.89.161] by tm8.bullet.mail.ne1.yahoo.com with NNFMP; 09 Feb 2011 08:32:39 -0000 23, 37 -- Received: from [127.0.0.1] by omp1017.mail.ne1.yahoo.com with NNFMP; 09 Feb 2011 08:32:39 -0000 23, 37 -- X-Yahoo-Newman-Property: ymail-3 23, 37 -- X-Yahoo-Newman-Id: 135987.33674.bm-at-omp1017.mail.ne1.yahoo.com 23, 37 -- Received: (qmail 43083 invoked by uid 60001); 9 Feb 2011 08:32:38 -0000 23, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297240358; bh=UbifmS8nD9YE3hYKq5Uoh7qxsDHmZxpvaTWryf/5JAw=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=zFZxd1y2CVth3qfuqs0m3L159q08hU2s17jhGa73gRYrD2LITyQGYPbFF7j4QJkWBuPMe6wp5ZAleem+k+ZSPeLrFGbjTaLHubwBh8F3zSc9Y8x+N2BKMTgh9NiirPg36PS/CFbcKuDSZ0nhowqtZeJzYKZRg8ESxXl1TObHg/M= 23, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 23, 37 -- s=s1024; d=yahoo.com; 23, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 23, 37 -- b=eQSzmAda5QtAo53DcoGaZFZCOCimYF3oQ7S5kQFOwZRhbpHiE8WkStYQZpuy0SOQjrwpfqmoiDHYC8si/Xs8FIMRJ3IeNXd8f1uVj9UkyF1vNAM23LmJsnIJ0+plNwtXOMK9jDMUTthkSgcHAq/7pPsifItehx9vBs89R2iMLz8=; 23, 37 -- Message-ID: {494737.41704.qm-at-web110807.mail.gq1.yahoo.com} 23, 37 -- X-YMail-OSG: j_0qbjsVM1kR.UVBJezbUvOMPSv8zzZibaNpkQjZuuKDEv8 23, 37 -- JMdJHyBeh6.6xbHz7I4c2EtBfnE1CgOQxB1wHAfXeWJ4uhZn07ISfJtvtMMs 23, 37 -- tJsApaOutO_jEGtLSKKrfT8cqxgWRHWyJfzbe74XfgWOe_zN5t0wD02HAB_R 23, 37 -- wN3eLV0U7GDR0LaE4ZWNqF1qOoK0qLfZYJe7CJKWEFK2ACCEDYzMzIfRUB.C 23, 37 -- .OP4JkXOpXDv9iBvfmfqOtX5eNNCbN3ldVsL6qpMpIdRJu8yTRvrcgZU3VuK 23, 37 -- xe.5xEMCOU3fhuBCp7dwcCANfpIvNBvfhhWgjVVuazUQQjv7maYHzEpqUBXD 23, 37 -- M2jxr6mEf7.8GA_03ihRXp5W37EquPVKJrRT.eA0azu8lFcfBEhOPBKrqpLJ 23, 37 -- qP9yO2AJEhGHCZHU- 23, 37 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Wed, 09 Feb 2011 00:32:38 PST 23, 37 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 23, 37 -- References: {201102081353.p18DrJMs001839-at-ns.microscopy.com} 23, 37 -- Date: Wed, 9 Feb 2011 00:32:38 -0800 (PST) 23, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 23, 37 -- Subject: Re: [Microscopy] Help with Sectioning Soil 23, 37 -- To: modla-at-dbi.udel.edu 23, 37 -- Cc: microscopy-at-microscopy.com 23, 37 -- In-Reply-To: {201102081353.p18DrJMs001839-at-ns.microscopy.com} 23, 37 -- MIME-Version: 1.0 23, 37 -- Content-Type: text/plain; charset=iso-8859-1 23, 37 -- Content-Transfer-Encoding: 8bit 23, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p198WdYp017228 ==============================End of - Headers==============================
You may want to have a look on the Facility Online Manager (FOM) system at http://www.fomnetworks.com/. The FOM system provides a complete solution from resource scheduling to billing and reporting. FOM is capable of managing unlimited number of facilities, instruments, and users. If you need scheduler-only functions, it costs $100 for life time use with free hosting.
Please contact me offline if you are interested. Thanks, Shuyou _________________ Shuyou Li, Ph.D. FOM Networks, Inc. www.fomnetworks.com 224-225-9168
-----Original Message----- X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu] Sent: Tuesday, February 08, 2011 2:39 PM To: syli-at-northwestern.edu
Our facility is currently using a Calcium calendar package (specifically Calcium 3.10.1 Professional 25 w/Email by Brown Bear Software) for online equipment scheduling. We're fairly pleased with it, but we've been notified of incompatibilities with upcoming changes to our website (programming and design, hosting, servers, etc). Unfortunately, I don't know the details at this time.
We're looking for something that's relatively inexpensive, secure, user-friendly, somewhat customizable, and allows us to send email notifications and export events to data files (which we can then import into Excel for recordkeeping, billing and analysis). And we need the option to administer calendars for at least ten different pieces of equipment.
We'd appreciate all the suggestions we can get. We'll take a look at them and run our selections past our webkeepers. (You may send responses directly to me offline.)
Thank you so much for your help,
Jaci
Jaclynn Lett Senior Research Technician, EM Facility Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S. Euclid Ave., Campus Box 8115 Saint Louis, MO 63110
Email: lettj-at-ent.wustl.edu Office: 314-747-7257 Fax: 314-747-7230 Website: http://otocore.wustl.edu This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail.
==============================Original Headers============================== 8, 23 -- From LettJ-at-ent.wustl.edu Tue Feb 8 14:35:42 2011 8, 23 -- Received: from MAIL4.wusm-pcf.wustl.edu (mail4.wusm-pcf.wustl.edu [128.252.17.171]) 8, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p18KZgfP005572 8, 23 -- for {microscopy-at-microscopy.com} ; Tue, 8 Feb 2011 14:35:42 -0600 8, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL4.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.4675); 8, 23 -- Tue, 8 Feb 2011 14:35:41 -0600 8, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 23 -- Content-class: urn:content-classes:message 8, 23 -- MIME-Version: 1.0 8, 23 -- Content-Type: text/plain; 8, 23 -- charset="us-ascii" 8, 23 -- Subject: FACILITIES online scheduling 8, 23 -- Date: Tue, 8 Feb 2011 14:35:42 -0600 8, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C3901E99687-at-EX04.wusm-pcf.wustl.edu} 8, 23 -- X-MS-Has-Attach: 8, 23 -- X-MS-TNEF-Correlator: 8, 23 -- Thread-Topic: FACILITIES online scheduling 8, 23 -- Thread-Index: AcvHz76b3PvcRBsUQtevV/+aRWBYQA== 8, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 8, 23 -- To: {microscopy-at-microscopy.com} 8, 23 -- X-OriginalArrivalTime: 08 Feb 2011 20:35:41.0708 (UTC) FILETIME=[C0CE14C0:01CBC7CF] 8, 23 -- Content-Transfer-Encoding: 8bit 8, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p18KZgfP005572 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 23 -- From syli-at-northwestern.edu Wed Feb 9 11:54:46 2011 18, 23 -- Received: from mailp5.ci.northwestern.edu (casbah.it.northwestern.edu [129.105.16.95]) 18, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p19HskYl030567 18, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Feb 2011 11:54:46 -0600 18, 23 -- Received: from ShuyouPC (prep.ms.northwestern.edu [129.105.37.239]) 18, 23 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 18, 23 -- (No client certificate requested) 18, 23 -- by mailp5.ci.northwestern.edu (Postfix) with ESMTP id B52D876DA9 18, 23 -- for {microscopy-at-microscopy.com} ; Wed, 9 Feb 2011 11:54:46 -0600 (CST) 18, 23 -- From: "Shuyou Li" {syli-at-northwestern.edu} 18, 23 -- To: {microscopy-at-microscopy.com} 18, 23 -- References: {201102082038.p18KcdP3010874-at-ns.microscopy.com} 18, 23 -- In-Reply-To: {201102082038.p18KcdP3010874-at-ns.microscopy.com} 18, 23 -- Subject: RE: [Microscopy] FACILITIES online scheduling 18, 23 -- Date: Wed, 9 Feb 2011 11:54:48 -0600 18, 23 -- Message-ID: {004b01cbc882$719b7bf0$54d273d0$-at-northwestern.edu} 18, 23 -- MIME-Version: 1.0 18, 23 -- Content-Type: text/plain; 18, 23 -- charset="us-ascii" 18, 23 -- Content-Transfer-Encoding: 7bit 18, 23 -- X-Mailer: Microsoft Outlook 14.0 18, 23 -- Thread-Index: AQHeth4bk4y5xdA4MxGoEGKsdoO1a5PTmCkA 18, 23 -- Content-Language: en-us ==============================End of - Headers==============================
Our lab uses the Faces Scheduling System, run by the Complex Carbohydrate Research Center at the University of Georgia. The schedule itself runs inside a Java applet, so it's accessible from any device or operating system. You can add users, assign permissions, and manage multiple instruments. The software is remotely hosted and free to use.
For more information visit: http://faces.ccrc.uga.edu/ccrcfaces/faq0.php4
-- Steven Spurgeon Dynamic Characterization Group Department of Materials Science & Engineering Drexel University
I have a request that is very different from my usual pleas for help. I'm putting together some educational materials on plant biology for the upcoming AAAS Family Science Days in Washington DC next weekend and I've hit a snag with some illustrations that I wanted to use - got permission, then just got a BIG bill. Given the timeline, putting together some new micrographs of my own is just not in the cards, though I'm looking, looking, looking through my files.
Would anyone out there be willing to donate light and/or TEM micrographs of the following? I will be sure to cite the donor in my materials and happy to send you an electronic copy of them.
1. a relatively high mag TEM of a chloroplast, the entire organelle 2. a light micrograph of chloroplasts 3. a light micrograph of a cross section of a dicot leaf 4. a light micrograph of a cross section of a dicot stem 5. a light micrograph of a longitudinal section of a dicot stem 6. a light micrograph of a cross section of a root 7. a light micrograph of a longitudinal section of a root
Specifically, these are going on an interactive poster exploring photosynthesis.
I put it together for the recent US Science & Engineering Festival on a small scale in my area and have been asked to make it available at the upcoming AAAS Family Science Days and on the American Society of Plant Biologist's education web resource. Anything that we can do to get the public more interested in science!
Thanks in advance for your help. If you are willing to share a micrograph, please send me an email. Please include how you would like to be cited in the materials in your email.
Best, Kristen Lennon Department of Biology Frostburg State University Frostburg, MD 21532 kalennon-at-frostburg.edu
==============================Original Headers============================== 12, 29 -- From kamlennon-at-yahoo.com Wed Feb 9 20:34:36 2011 12, 29 -- Received: from web84002.mail.mud.yahoo.com (web84002.mail.mud.yahoo.com [68.142.206.172]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1A2YalA011158 12, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Feb 2011 20:34:36 -0600 12, 29 -- Received: (qmail 50850 invoked by uid 60001); 10 Feb 2011 02:34:36 -0000 12, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297305275; bh=0gytRI+ms27mua5smpFQj74nG7yiQ3T/jhlsl6dg62Y=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=3jcSrj++3MnKjVRmqPxKPCzLUVqdKVL3sMk3pzF67Zv8csrBmS929Qzd/q/R+Y1g5fsbtpDgQ2KceK+kZ3klHmKOM4yCBKac4dcRFdh4Cv1mqR0E9A28sl7kVRIvroOT2M6vS20Fc356MeCBce81fx+6T3OLl7yvdfgfU36DBCE= 12, 29 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 29 -- s=s1024; d=yahoo.com; 12, 29 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 12, 29 -- b=12wcW5+lQoIrphIX0/WVZbfka/5udkNSXLIGyU7QYlxbnMNOJySQAZchT1Q+uLLGrjQ+uK88UEw2o7+EkQIK+rS+D/OMFDDTWWKyQY7eiLh7T9I+nIyGWK52rN09HhyLAvZVuyPBpCvz/F+7vlzRXZIhC7Rg4fwT8pQ5Nto9Moo=; 12, 29 -- Message-ID: {966802.50831.qm-at-web84002.mail.mud.yahoo.com} 12, 29 -- X-YMail-OSG: Drr2ZjYVM1m9ZcFK9zy9nZgh4zvoGxAElGfdfsUly6AMBDa 12, 29 -- Yr4Et4sMl9zifQRR6wsSGgiPAhwjvVRjy_6y3TIFppSLIBgLEUx57AUZJYIt 12, 29 -- g1pIwiOXr0Qf5CBGY12Dn8crwWOSgT9eqw6R6AXvQqsncaU2xW08M0JxEmNr 12, 29 -- C1agPXyqEbLqCXZUY2gjNq9VryCbawbqn88ojGapNMY0Y6naL8vWmhlzW_HT 12, 29 -- aDCpz5TVgciSxiqMYRr5afptiR_U3etmPRjJJs7eac0TlQEzM4baH65zuOzg 12, 29 -- Zw508oyMHxyD3Zcicfii6h.iy5Z2TWZ2D3uIV89XGChCyhkiEiV1SR0QFeHE 12, 29 -- nAdMqGOd9ep4UorCxanCK4eWExcGuifvSqU12DFvDdHWARU7Ngb.Rssze0pg 12, 29 -- 2TwuzTNeVNIue 12, 29 -- Received: from [96.239.134.126] by web84002.mail.mud.yahoo.com via HTTP; Wed, 09 Feb 2011 18:34:35 PST 12, 29 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 12, 29 -- Date: Wed, 9 Feb 2011 18:34:35 -0800 (PST) 12, 29 -- From: Kristen Lennon {kamlennon-at-yahoo.com} 12, 29 -- Subject: plant micrographs needed 12, 29 -- To: microscopy-at-microscopy.com 12, 29 -- MIME-Version: 1.0 12, 29 -- Content-Type: text/plain; charset=iso-8859-1 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1A2YalA011158 ==============================End of - Headers==============================
Sorry if this message is not directly related to microscopy but it is definitely related to scientific work. We are looking for a software to manage our scientific literature. Commercial reknown software like Endnote or reference manager are la creme de la creme but it comes to a price. I was wondering if a free or low-cost software could not fulfill the task as well. What we want/want not:
- Possibility to share the database between numerous computers. - Database saved on our server, not on the internet - Integration with microsoft word would be nice but not necessary - Possibility to retrieve the references directly from Pubmed would be nice but not necessary
I know there is a nice comparison page in wikipedia but I am a little bit limited to inderstand the vocabulary used (web-based and so on) and also it would be interesting to get the opinion of experienced users.
Many thanks in advance.
Stephane
==============================Original Headers============================== 9, 34 -- From nizets2-at-yahoo.com Thu Feb 10 07:16:40 2011 9, 34 -- Received: from nm6-vm0.bullet.mail.sp2.yahoo.com (nm6-vm0.bullet.mail.sp2.yahoo.com [98.139.91.206]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1ADGeAg012338 9, 34 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 07:16:40 -0600 9, 34 -- Received: from [98.139.91.61] by nm6.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- Received: from [98.139.91.48] by tm1.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- Received: from [127.0.0.1] by omp1048.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- X-Yahoo-Newman-Property: ymail-3 9, 34 -- X-Yahoo-Newman-Id: 956507.36688.bm-at-omp1048.mail.sp2.yahoo.com 9, 34 -- Received: (qmail 33202 invoked by uid 60001); 10 Feb 2011 13:16:39 -0000 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297343799; bh=tNHcQ9jkH2iVK6ueVLJJCYPBVBFzCTQaU+JhgiMXp1k=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=df0kyelfOMBu3f6xF23nRcU+xJSDsucjrErMEA3SfAQ03nrW5HSofXmAC+3jdwI/g/ShUYUYUw7r7flOzz3o/vq77ZOnW6XYnRtZGX7fg45H/KH+I1qG2xCMFjPNIk0N93kmEvHVSjkldkPOyETpyHVPskzgdaHt2FvEpKt9FHo= 9, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 34 -- s=s1024; d=yahoo.com; 9, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 9, 34 -- b=UzFS8svM8Y/B+7qP/Q/c5DSECyhFf6TZA8lThkmYbw52LynZzAAzDAcKcUd23cgsoJmiRvhrts1AnmUtuian/x/HKHy/ERTRPbhILAAZuJXI9NtgXqykUyAuQlhcFqKcXQCVjWVC8hWxWPzLmcOEaZLi9zFPKNKy+QOsl5+QrZ0=; 9, 34 -- Message-ID: {339537.10240.qm-at-web110805.mail.gq1.yahoo.com} 9, 34 -- X-YMail-OSG: qaRsTmoVM1lvgJBqqtJ0oHlctXIXm8k_7L_6p.jD3ZSuqRq 9, 34 -- 0Pr1PagNdTjzcFmqQBOU8PscC6c_svU4lkWxMTQM.5skIF12SqleEMSppADt 9, 34 -- BpMoMSAonk2MLJ0Wd0MgE20anLCSmr.1Bz96r9SILsXGymxBF1JGiP6hHHnq 9, 34 -- 6s6CR5m7PP5hexpEu2V7NKo6helkqNeMOnvtZmB4q5xEnKL7lmadpvBq8sLi 9, 34 -- 3n1i9hewK9HtG4dW9lUC8VjGfyi8eEEXw1OcBmbkNNE_Mh26.UYcO9C_U.98 9, 34 -- _UJpzs6QKrWL70rCmir6M0oACKcTTzEQw8xZuDQ5Y3oW1B5NIDM87rFwhg0Y 9, 34 -- wfyt9PhlW.O3J8mrKARhRr2bP9KougD8rdsLWM.h8qtO1m6EnQHZqaFfCwAV 9, 34 -- DvlwYrxAXK400 9, 34 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 10 Feb 2011 05:16:39 PST 9, 34 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 9, 34 -- Date: Thu, 10 Feb 2011 05:16:39 -0800 (PST) 9, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 34 -- Subject: reference manager software 9, 34 -- To: microscopy-at-microscopy.com 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; charset=iso-8859-1 9, 34 -- Content-Transfer-Encoding: 8bit 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ADGeAg012338 ==============================End of - Headers==============================
Stephane, A wonderful and highly regarded reference managing software is or was Papyrus. I say is or was because this was supported for Mac OS 9 but not OS 10. As a mac user I don't know if it remains supported for the latest versions of Windows. It could do *anything* and did it with a sense of humor.
As a MacOS 10 person, I use software called Bookends. I like it just fine, although there are a few things I miss from Papyrus. It does all the things you ask for. I dont know if there is a PC version. It is not free but I found the cost reasonable.
Hope this helps, Tobias
} } } Dear all, } } Sorry if this message is not directly related to } microscopy but it is definitely } related to scientific work. } We are looking for aÝsoftware to manage our scientific literature. } Commercial reknown software like Endnote or } reference manager are la creme de la } creme but it comes to a price. } I was wondering if a free or low-cost software could not fulfill the task as } well. } What we want/want not: } } - Possibility to share the database between numerous computers. } - Database saved on our server, not on the internet } - Integration with microsoft word would be nice but not necessary } - Possibility to retrieve the references } directly from Pubmed would be nice but } not necessary } } I know there is a nice comparison page in wikipedia but I am a little bit } limited to inderstand the vocabulary used (web-based and so on) and also it } would be interesting to get the opinion of experienced users. } } Many thanks in advance. } } Stephane } }
Try Medeley at the following link: http://www.mendeley.com/
Its free, can save the references to internet and your local HD, can link the PDF's, works with Microsft Office, will import citations from Science Direct, ISI Web of Science. You can also share libraries with colleagues and there is a development forum where users can suggest new functionality.
I'm a DPhil student in Materials, I like it, and most of my colleagues also like and use it.
Try it out you have nothing to lose and reference management to gain.
Cheers George
________________________________________ X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com] Sent: 10 February 2011 13:26 To: George Theodossiou
Dear all,
Sorry if this message is not directly related to microscopy but it is definitely related to scientific work. We are looking for a software to manage our scientific literature. Commercial reknown software like Endnote or reference manager are la creme de la creme but it comes to a price. I was wondering if a free or low-cost software could not fulfill the task as well. What we want/want not:
- Possibility to share the database between numerous computers. - Database saved on our server, not on the internet - Integration with microsoft word would be nice but not necessary - Possibility to retrieve the references directly from Pubmed would be nice but not necessary
I know there is a nice comparison page in wikipedia but I am a little bit limited to inderstand the vocabulary used (web-based and so on) and also it would be interesting to get the opinion of experienced users.
Many thanks in advance.
Stephane
==============================Original Headers============================== 9, 34 -- From nizets2-at-yahoo.com Thu Feb 10 07:16:40 2011 9, 34 -- Received: from nm6-vm0.bullet.mail.sp2.yahoo.com (nm6-vm0.bullet.mail.sp2.yahoo.com [98.139.91.206]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1ADGeAg012338 9, 34 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 07:16:40 -0600 9, 34 -- Received: from [98.139.91.61] by nm6.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- Received: from [98.139.91.48] by tm1.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- Received: from [127.0.0.1] by omp1048.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- X-Yahoo-Newman-Property: ymail-3 9, 34 -- X-Yahoo-Newman-Id: 956507.36688.bm-at-omp1048.mail.sp2.yahoo.com 9, 34 -- Received: (qmail 33202 invoked by uid 60001); 10 Feb 2011 13:16:39 -0000 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297343799; bh=tNHcQ9jkH2iVK6ueVLJJCYPBVBFzCTQaU+JhgiMXp1k=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=df0kyelfOMBu3f6xF23nRcU+xJSDsucjrErMEA3SfAQ03nrW5HSofXmAC+3jdwI/g/ShUYUYUw7r7flOzz3o/vq77ZOnW6XYnRtZGX7fg45H/KH+I1qG2xCMFjPNIk0N93kmEvHVSjkldkPOyETpyHVPskzgdaHt2FvEpKt9FHo= 9, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 34 -- s=s1024; d=yahoo.com; 9, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 9, 34 -- b=UzFS8svM8Y/B+7qP/Q/c5DSECyhFf6TZA8lThkmYbw52LynZzAAzDAcKcUd23cgsoJmiRvhrts1AnmUtuian/x/HKHy/ERTRPbhILAAZuJXI9NtgXqykUyAuQlhcFqKcXQCVjWVC8hWxWPzLmcOEaZLi9zFPKNKy+QOsl5+QrZ0=; 9, 34 -- Message-ID: {339537.10240.qm-at-web110805.mail.gq1.yahoo.com} 9, 34 -- X-YMail-OSG: qaRsTmoVM1lvgJBqqtJ0oHlctXIXm8k_7L_6p.jD3ZSuqRq 9, 34 -- 0Pr1PagNdTjzcFmqQBOU8PscC6c_svU4lkWxMTQM.5skIF12SqleEMSppADt 9, 34 -- BpMoMSAonk2MLJ0Wd0MgE20anLCSmr.1Bz96r9SILsXGymxBF1JGiP6hHHnq 9, 34 -- 6s6CR5m7PP5hexpEu2V7NKo6helkqNeMOnvtZmB4q5xEnKL7lmadpvBq8sLi 9, 34 -- 3n1i9hewK9HtG4dW9lUC8VjGfyi8eEEXw1OcBmbkNNE_Mh26.UYcO9C_U.98 9, 34 -- _UJpzs6QKrWL70rCmir6M0oACKcTTzEQw8xZuDQ5Y3oW1B5NIDM87rFwhg0Y 9, 34 -- wfyt9PhlW.O3J8mrKARhRr2bP9KougD8rdsLWM.h8qtO1m6EnQHZqaFfCwAV 9, 34 -- DvlwYrxAXK400 9, 34 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 10 Feb 2011 05:16:39 PST 9, 34 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 9, 34 -- Date: Thu, 10 Feb 2011 05:16:39 -0800 (PST) 9, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 34 -- Subject: reference manager software 9, 34 -- To: microscopy-at-microscopy.com 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; charset=iso-8859-1 9, 34 -- Content-Transfer-Encoding: 8bit 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ADGeAg012338 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 30 -- From george.theodossiou-at-st-annes.ox.ac.uk Thu Feb 10 07:47:19 2011 19, 30 -- Received: from relay5.mail.ox.ac.uk (relay5.mail.ox.ac.uk [163.1.2.163]) 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ADlIK8012299 19, 30 -- for {Microscopy-at-Microscopy.com} ; Thu, 10 Feb 2011 07:47:18 -0600 19, 30 -- Received: from smtp1.nexus.ox.ac.uk ([163.1.154.135] helo=exht01.ad.oak.ox.ac.uk) 19, 30 -- by relay5.mail.ox.ac.uk with esmtp (Exim 4.71) 19, 30 -- (envelope-from {george.theodossiou-at-st-annes.ox.ac.uk} ) 19, 30 -- id 1PnWrd-0002h3-J2 19, 30 -- for Microscopy-at-Microscopy.com; Thu, 10 Feb 2011 13:47:17 +0000 19, 30 -- Received: from EXMBX01.ad.oak.ox.ac.uk ([169.254.2.11]) by 19, 30 -- exht01.ad.oak.ox.ac.uk ([163.1.154.52]) with mapi; Thu, 10 Feb 2011 13:47:18 19, 30 -- +0000 19, 30 -- From: George Theodossiou {george.theodossiou-at-st-annes.ox.ac.uk} 19, 30 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 19, 30 -- Date: Thu, 10 Feb 2011 13:47:18 +0000 19, 30 -- Subject: RE: [Microscopy] reference manager software 19, 30 -- Thread-Topic: [Microscopy] reference manager software 19, 30 -- Thread-Index: AcvJJio3PY2dexRATK2HdcdG3cIPVQAAVzlX 19, 30 -- Message-ID: {CFADFFD51D0744438FC8E1A5A990E4DF891C1FE399-at-EXMBX01.ad.oak.ox.ac.uk} 19, 30 -- References: {201102101326.p1ADQii1025650-at-ns.microscopy.com} 19, 30 -- In-Reply-To: {201102101326.p1ADQii1025650-at-ns.microscopy.com} 19, 30 -- Accept-Language: en-US, en-GB 19, 30 -- Content-Language: en-GB 19, 30 -- X-MS-Has-Attach: 19, 30 -- X-MS-TNEF-Correlator: 19, 30 -- acceptlanguage: en-US, en-GB 19, 30 -- Content-Type: text/plain; charset="us-ascii" 19, 30 -- MIME-Version: 1.0 19, 30 -- Content-Transfer-Encoding: 8bit 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ADlIK8012299 ==============================End of - Headers==============================
Some that I have fooled around with are I,Librarian (http://www.bioinformatics.org/librarian/), Paperpile (http://paperpile.com), Mendeley (http://www.mendeley.com/), Zotero (http://www.zotero.org/), and Papers (http://www.mendeley.com/). Of these, I currently use Mendeley for Windows and Papers for OS X. That said, I,Librarian seems to be what you are after as far as being server-based and cross-platform and it looks to have most of what you are after in regard to functionality. Paperpile is a nice analog to Papers that is free and cross platform, but still in beta. Mendeley, and this is my personal opinion only, is only okay at best. There is a lot of basic functionality that it lacks and the server support you want isn't free. Zotero is pretty feature rich but has the same issue of limited free server space. Papers is Mac only and not free, but it has become the defacto metric for all the others. I haven't seen a review of one of these applications in recent years that doesn't include a comparison to it. I hope that helps!
Best,
Mike Anderson (Please note that this is an expression of personal preference and not official endorsement by NIST.)
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Thursday, February 10, 2011 8:28 AM To: Anderson, Michael
Dear all,
Sorry if this message is not directly related to microscopy but it is definitely related to scientific work. We are looking for a software to manage our scientific literature. Commercial reknown software like Endnote or reference manager are la creme de la creme but it comes to a price. I was wondering if a free or low-cost software could not fulfill the task as well. What we want/want not:
- Possibility to share the database between numerous computers. - Database saved on our server, not on the internet - Integration with microsoft word would be nice but not necessary - Possibility to retrieve the references directly from Pubmed would be nice but not necessary
I know there is a nice comparison page in wikipedia but I am a little bit limited to inderstand the vocabulary used (web-based and so on) and also it would be interesting to get the opinion of experienced users.
Many thanks in advance.
Stephane
==============================Original Headers============================== 9, 34 -- From nizets2-at-yahoo.com Thu Feb 10 07:16:40 2011 9, 34 -- Received: from nm6-vm0.bullet.mail.sp2.yahoo.com (nm6-vm0.bullet.mail.sp2.yahoo.com [98.139.91.206]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1ADGeAg012338 9, 34 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 07:16:40 -0600 9, 34 -- Received: from [98.139.91.61] by nm6.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- Received: from [98.139.91.48] by tm1.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- Received: from [127.0.0.1] by omp1048.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 9, 34 -- X-Yahoo-Newman-Property: ymail-3 9, 34 -- X-Yahoo-Newman-Id: 956507.36688.bm-at-omp1048.mail.sp2.yahoo.com 9, 34 -- Received: (qmail 33202 invoked by uid 60001); 10 Feb 2011 13:16:39 -0000 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297343799; bh=tNHcQ9jkH2iVK6ueVLJJCYPBVBFzCTQaU+JhgiMXp1k=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=df0kyelfOMBu3f6xF23nRcU+xJSDsucjrErMEA3SfAQ03nrW5HSofXmAC+3jdwI/g/ShUYUYUw7r7flOzz3o/vq77ZOnW6XYnRtZGX7fg45H/KH+I1qG2xCMFjPNIk0N93kmEvHVSjkldkPOyETpyHVPskzgdaHt2FvEpKt9FHo= 9, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 34 -- s=s1024; d=yahoo.com; 9, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 9, 34 -- b=UzFS8svM8Y/B+7qP/Q/c5DSECyhFf6TZA8lThkmYbw52LynZzAAzDAcKcUd23cgsoJmiRvhrts1AnmUtuian/x/HKHy/ERTRPbhILAAZuJXI9NtgXqykUyAuQlhcFqKcXQCVjWVC8hWxWPzLmcOEaZLi9zFPKNKy+QOsl5+QrZ0=; 9, 34 -- Message-ID: {339537.10240.qm-at-web110805.mail.gq1.yahoo.com} 9, 34 -- X-YMail-OSG: qaRsTmoVM1lvgJBqqtJ0oHlctXIXm8k_7L_6p.jD3ZSuqRq 9, 34 -- 0Pr1PagNdTjzcFmqQBOU8PscC6c_svU4lkWxMTQM.5skIF12SqleEMSppADt 9, 34 -- BpMoMSAonk2MLJ0Wd0MgE20anLCSmr.1Bz96r9SILsXGymxBF1JGiP6hHHnq 9, 34 -- 6s6CR5m7PP5hexpEu2V7NKo6helkqNeMOnvtZmB4q5xEnKL7lmadpvBq8sLi 9, 34 -- 3n1i9hewK9HtG4dW9lUC8VjGfyi8eEEXw1OcBmbkNNE_Mh26.UYcO9C_U.98 9, 34 -- _UJpzs6QKrWL70rCmir6M0oACKcTTzEQw8xZuDQ5Y3oW1B5NIDM87rFwhg0Y 9, 34 -- wfyt9PhlW.O3J8mrKARhRr2bP9KougD8rdsLWM.h8qtO1m6EnQHZqaFfCwAV 9, 34 -- DvlwYrxAXK400 9, 34 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 10 Feb 2011 05:16:39 PST 9, 34 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 9, 34 -- Date: Thu, 10 Feb 2011 05:16:39 -0800 (PST) 9, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 34 -- Subject: reference manager software 9, 34 -- To: microscopy-at-microscopy.com 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; charset=iso-8859-1 9, 34 -- Content-Transfer-Encoding: 8bit 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ADGeAg012338 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 30 -- From michael.anderson-at-nist.gov Thu Feb 10 07:51:22 2011 19, 30 -- Received: from smtp.nist.gov (rimp1.nist.gov [129.6.16.226]) 19, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ADpMj1020059 19, 30 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 07:51:22 -0600 19, 30 -- Received: from WSXGHUB1.xchange.nist.gov (WSXGHUB1.xchange.nist.gov [129.6.18.96]) 19, 30 -- by smtp.nist.gov (8.13.1/8.13.1) with ESMTP id p1ADpCX1002960 19, 30 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 08:51:13 -0500 19, 30 -- Received: from MBCLUSTER.xchange.nist.gov ([fe80::41df:f63f:c718:e08]) by 19, 30 -- WSXGHUB1.xchange.nist.gov ([129.6.18.96]) with mapi; Thu, 10 Feb 2011 19, 30 -- 08:51:12 -0500 19, 30 -- From: "Anderson, Michael" {michael.anderson-at-nist.gov} 19, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 30 -- Date: Thu, 10 Feb 2011 08:51:09 -0500 19, 30 -- Subject: RE: [Microscopy] reference manager software 19, 30 -- Thread-Topic: [Microscopy] reference manager software 19, 30 -- Thread-Index: AcvJJlS0k2dl1QVUQXKBuCcdGR6FeQAADCTw 19, 30 -- Message-ID: {7E80450DE7474E4881E405C742AC0DEA11DC500761-at-MBCLUSTER.xchange.nist.gov} 19, 30 -- References: {201102101328.p1ADS28l027778-at-ns.microscopy.com} 19, 30 -- In-Reply-To: {201102101328.p1ADS28l027778-at-ns.microscopy.com} 19, 30 -- Accept-Language: en-US 19, 30 -- Content-Language: en-US 19, 30 -- X-MS-Has-Attach: 19, 30 -- X-MS-TNEF-Correlator: 19, 30 -- acceptlanguage: en-US 19, 30 -- Content-Type: text/plain; charset="iso-8859-1" 19, 30 -- MIME-Version: 1.0 19, 30 -- X-NIST-MailScanner: Found to be clean 19, 30 -- X-NIST-MailScanner-From: michael.anderson-at-nist.gov 19, 30 -- Content-Transfer-Encoding: 8bit 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ADpMj1020059 ==============================End of - Headers==============================
We have some images in The Cell: An Image Library(tm) www.cellimagelibrary.org that you might find useful and many of them are in the public domain, so free for you to use.
1. Just enter chloroplast in the simple search box at the top of the homepage.
As to the others, unfortunately, we do not yet have a lot of plant cell images. Please do consider submitting yours to The Library when you have the time.
If I can be of further assistance please let me know.
David
David Orloff Manager, Image Library The American Society for Cell Biology The science of life, the life of science 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 T: 301-347-9300/Direct: 301-347-9305 F: 301-347-9310 E-mail: dorloff-at-ascb.org Web site: www.ascb.org The Cell: An Image LibraryT: www.cellimagelibrary.org LinkedIn Group: http://www.linkedin.com/groupRegistration?gid=3733425
-----Original Message----- X-from: kamlennon-at-yahoo.com [mailto:kamlennon-at-yahoo.com] Sent: Wednesday, February 09, 2011 9:43 PM To: David Orloff
Hi Listers,
I have a request that is very different from my usual pleas for help. I'm putting together some educational materials on plant biology for the upcoming AAAS Family Science Days in Washington DC next weekend and I've hit a snag with some illustrations that I wanted to use - got permission, then just got a BIG bill. Given the timeline, putting together some new micrographs of my own is just not in the cards, though I'm looking, looking, looking through my files.
Would anyone out there be willing to donate light and/or TEM micrographs of the following? I will be sure to cite the donor in my materials and happy to send you an electronic copy of them.
1. a relatively high mag TEM of a chloroplast, the entire organelle 2. a light micrograph of chloroplasts 3. a light micrograph of a cross section of a dicot leaf 4. a light micrograph of a cross section of a dicot stem 5. a light micrograph of a longitudinal section of a dicot stem 6. a light micrograph of a cross section of a root 7. a light micrograph of a longitudinal section of a root
Specifically, these are going on an interactive poster exploring photosynthesis.
I put it together for the recent US Science & Engineering Festival on a small scale in my area and have been asked to make it available at the upcoming AAAS Family Science Days and on the American Society of Plant Biologist's education web resource. Anything that we can do to get the public more interested in science!
Thanks in advance for your help. If you are willing to share a micrograph, please send me an email. Please include how you would like to be cited in the materials in your email.
Best, Kristen Lennon Department of Biology Frostburg State University Frostburg, MD 21532 kalennon-at-frostburg.edu
==============================Original Headers============================== 12, 29 -- From kamlennon-at-yahoo.com Wed Feb 9 20:34:36 2011 12, 29 -- Received: from web84002.mail.mud.yahoo.com (web84002.mail.mud.yahoo.com [68.142.206.172]) 12, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1A2YalA011158 12, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Feb 2011 20:34:36 -0600 12, 29 -- Received: (qmail 50850 invoked by uid 60001); 10 Feb 2011 02:34:36 -0000 12, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297305275; bh=0gytRI+ms27mua5smpFQj74nG7yiQ3T/jhlsl6dg62Y=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=3jcSrj++3MnKjVRmqPxKPCzLUVqdKVL3sMk3pzF67Zv8csrBmS929Qzd/q/R+Y1g5fsbtpDgQ2KceK+kZ3klHmKOM4yCBKac4dcRFdh4Cv1mqR0E9A28sl7kVRIvroOT2M6vS20Fc356MeCBce81fx+6T3OLl7yvdfgfU36DBCE= 12, 29 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 12, 29 -- s=s1024; d=yahoo.com; 12, 29 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 12, 29 -- b=12wcW5+lQoIrphIX0/WVZbfka/5udkNSXLIGyU7QYlxbnMNOJySQAZchT1Q+uLLGrjQ+uK88UEw2o7+EkQIK+rS+D/OMFDDTWWKyQY7eiLh7T9I+nIyGWK52rN09HhyLAvZVuyPBpCvz/F+7vlzRXZIhC7Rg4fwT8pQ5Nto9Moo=; 12, 29 -- Message-ID: {966802.50831.qm-at-web84002.mail.mud.yahoo.com} 12, 29 -- X-YMail-OSG: Drr2ZjYVM1m9ZcFK9zy9nZgh4zvoGxAElGfdfsUly6AMBDa 12, 29 -- Yr4Et4sMl9zifQRR6wsSGgiPAhwjvVRjy_6y3TIFppSLIBgLEUx57AUZJYIt 12, 29 -- g1pIwiOXr0Qf5CBGY12Dn8crwWOSgT9eqw6R6AXvQqsncaU2xW08M0JxEmNr 12, 29 -- C1agPXyqEbLqCXZUY2gjNq9VryCbawbqn88ojGapNMY0Y6naL8vWmhlzW_HT 12, 29 -- aDCpz5TVgciSxiqMYRr5afptiR_U3etmPRjJJs7eac0TlQEzM4baH65zuOzg 12, 29 -- Zw508oyMHxyD3Zcicfii6h.iy5Z2TWZ2D3uIV89XGChCyhkiEiV1SR0QFeHE 12, 29 -- nAdMqGOd9ep4UorCxanCK4eWExcGuifvSqU12DFvDdHWARU7Ngb.Rssze0pg 12, 29 -- 2TwuzTNeVNIue 12, 29 -- Received: from [96.239.134.126] by web84002.mail.mud.yahoo.com via HTTP; Wed, 09 Feb 2011 18:34:35 PST 12, 29 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 12, 29 -- Date: Wed, 9 Feb 2011 18:34:35 -0800 (PST) 12, 29 -- From: Kristen Lennon {kamlennon-at-yahoo.com} 12, 29 -- Subject: plant micrographs needed 12, 29 -- To: microscopy-at-microscopy.com 12, 29 -- MIME-Version: 1.0 12, 29 -- Content-Type: text/plain; charset=iso-8859-1 12, 29 -- Content-Transfer-Encoding: 8bit 12, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1A2YalA011158 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 26 -- From DOrloff-at-ascb.org Thu Feb 10 08:27:37 2011 28, 26 -- Received: from smtp.ascb.org (coalitionforlifesciences.org [173.13.233.102] (may be forged)) 28, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1AERbJI012377 28, 26 -- for {Microscopy-at-Microscopy.Com} ; Thu, 10 Feb 2011 08:27:37 -0600 28, 26 -- Received: from ASCB2.ascb.local ([::1]) by ASCB2.ascb.local ([::1]) with mapi; 28, 26 -- Thu, 10 Feb 2011 09:27:34 -0500 28, 26 -- From: David Orloff {DOrloff-at-ascb.org} 28, 26 -- To: "'kamlennon-at-yahoo.com'" {kamlennon-at-yahoo.com} , 28, 26 -- "'Microscopy-at-Microscopy.Com'" {Microscopy-at-Microscopy.Com} 28, 26 -- CC: David Orloff {DOrloff-at-ascb.org} 28, 26 -- Date: Thu, 10 Feb 2011 09:27:33 -0500 28, 26 -- Subject: RE: [Microscopy] plant micrographs needed 28, 26 -- Thread-Topic: [Microscopy] plant micrographs needed 28, 26 -- Thread-Index: AcvIzDUjWgdpTG7wSFelEcJJN1rF2wAYZTFw 28, 26 -- Message-ID: {8D9C2A7731877D45B1691E78451EC3BE5C15F530-at-ASCB2.ascb.local} 28, 26 -- References: {201102100242.p1A2gigC026074-at-ns.microscopy.com} 28, 26 -- In-Reply-To: {201102100242.p1A2gigC026074-at-ns.microscopy.com} 28, 26 -- Accept-Language: en-US 28, 26 -- Content-Language: en-US 28, 26 -- X-MS-Has-Attach: 28, 26 -- X-MS-TNEF-Correlator: 28, 26 -- acceptlanguage: en-US 28, 26 -- Content-Type: text/plain; charset="iso-8859-1" 28, 26 -- MIME-Version: 1.0 28, 26 -- Content-Transfer-Encoding: 8bit 28, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1AERbJI012377 ==============================End of - Headers==============================
Helo Stephane, I am using JabRef, an open source bibliography reference manager. http://jabref.sourceforge.net/
It is a Java based. It's native file format is BibTeX. It can be run instantly with Java Web Start or it can be installed locally. It can download references directly from PubMed. It can import references from EndNote, etc. I am not sure about its integration with MS Word, but it can be integrated with Open Office or with LyX and other LaTex based editors.
Best regards Oldrich
On Thursday 10 of February 2011 14:18:57 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } Dear all, } } Sorry if this message is not directly related to microscopy but it is } definitely related to scientific work. } We are looking for a software to manage our scientific literature. } Commercial reknown software like Endnote or reference manager are la creme } de la creme but it comes to a price. } I was wondering if a free or low-cost software could not fulfill the task } as well. } What we want/want not: } } - Possibility to share the database between numerous computers. } - Database saved on our server, not on the internet } - Integration with microsoft word would be nice but not necessary } - Possibility to retrieve the references directly from Pubmed would be nice } but not necessary } } I know there is a nice comparison page in wikipedia but I am a little bit } limited to inderstand the vocabulary used (web-based and so on) and also it } would be interesting to get the opinion of experienced users. } } Many thanks in advance. } } Stephane }
==============================Original Headers============================== 5, 22 -- From benada-at-biomed.cas.cz Thu Feb 10 08:31:14 2011 5, 22 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1AEVEa4018166 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 08:31:14 -0600 5, 22 -- Received: from u117ob.localnet (u117ob.mbu.cas.cz [147.231.44.101]) 5, 22 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 5, 22 -- (No client certificate requested) 5, 22 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 83068930001 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 15:31:13 +0100 (CET) 5, 22 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 5, 22 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- Subject: Re: [Microscopy] reference manager software 5, 22 -- Date: Thu, 10 Feb 2011 15:31:02 +0100 5, 22 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 5, 22 -- References: {201102101318.p1ADIvcX015700-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201102101318.p1ADIvcX015700-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: Text/Plain; 5, 22 -- charset="windows-1250" 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- Message-Id: {201102101531.03324.benada-at-biomed.cas.cz} ==============================End of - Headers==============================
We use a locally developed resource, On-Core Facility Scheduler (OCF). It is open-source, and freely available from the Biocomputing facility here at the University of Arizona. It does require Linux and probably a few other things. It's used extensively to schedule all sorts of service labs here and at other universities. There are some very nice administrative features to the software. If your instrument runs on MS Windows, there's even a bit of software that runs in Windows that will report back to OCF server when a user has logged on/off, which helps with calculating the monthly billing. It's a nice bit of software, not perfect, but pretty darn good.
Contacts for the software: http://bcf.arl.arizona.edu/contact-us/index.php
Demo/downloads: http://demo.arl.arizona.edu/
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
-----Original Message----- X-from: spurgeon-at-drexel.edu [mailto:spurgeon-at-drexel.edu] Sent: Wednesday, February 09, 2011 6:11 PM To: Cromey, Douglas W - (dcromey)
Our lab uses the Faces Scheduling System, run by the Complex Carbohydrate Research Center at the University of Georgia. The schedule itself runs inside a Java applet, so it's accessible from any device or operating system. You can add users, assign permissions, and manage multiple instruments. The software is remotely hosted and free to use.
For more information visit: http://faces.ccrc.uga.edu/ccrcfaces/faq0.php4
-- Steven Spurgeon Dynamic Characterization Group Department of Materials Science & Engineering Drexel University
Bibus (http://bibus-biblio.sourceforge.net/) is a cross platform reference manager compatible with MS word, OpenOffice.org and Pubmed. It also has MySQL and SQlite compatibility so the database can either be shared on a server or directly on the local computer.
On Thu, 2011-02-10 at 07:26 -0600, nizets2-at-yahoo.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } Sorry if this message is not directly related to microscopy but it is definitely } related to scientific work. } We are looking for asoftware to manage our scientific literature. } Commercial reknown software like Endnote or reference manager are la creme de la } creme but it comes to a price. } I was wondering if a free or low-cost software could not fulfill the task as } well. } What we want/want not: } } - Possibility to share the database between numerous computers. } - Database saved on our server, not on the internet } - Integration with microsoft word would be nice but not necessary } - Possibility to retrieve the references directly from Pubmed would be nice but } not necessary } } I know there is a nice comparison page in wikipedia but I am a little bit } limited to inderstand the vocabulary used (web-based and so on) and also it } would be interesting to get the opinion of experienced users. } } Many thanks in advance. } } Stephane } } } } } } ==============================Original Headers============================== } 9, 34 -- From nizets2-at-yahoo.com Thu Feb 10 07:16:40 2011 } 9, 34 -- Received: from nm6-vm0.bullet.mail.sp2.yahoo.com (nm6-vm0.bullet.mail.sp2.yahoo.com [98.139.91.206]) } 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1ADGeAg012338 } 9, 34 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 07:16:40 -0600 } 9, 34 -- Received: from [98.139.91.61] by nm6.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 } 9, 34 -- Received: from [98.139.91.48] by tm1.bullet.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 } 9, 34 -- Received: from [127.0.0.1] by omp1048.mail.sp2.yahoo.com with NNFMP; 10 Feb 2011 13:16:39 -0000 } 9, 34 -- X-Yahoo-Newman-Property: ymail-3 } 9, 34 -- X-Yahoo-Newman-Id: 956507.36688.bm-at-omp1048.mail.sp2.yahoo.com } 9, 34 -- Received: (qmail 33202 invoked by uid 60001); 10 Feb 2011 13:16:39 -0000 } 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297343799; bh=tNHcQ9jkH2iVK6ueVLJJCYPBVBFzCTQaU+JhgiMXp1k=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=df0kyelfOMBu3f6xF23nRcU+xJSDsucjrErMEA3SfAQ03nrW5HSofXmAC+3jdwI/g/ShUYUYUw7r7flOzz3o/vq77ZOnW6XYnRtZGX7fg45H/KH+I1qG2xCMFjPNIk0N93kmEvHVSjkldkPOyETpyHVPskzgdaHt2FvEpKt9FHo= } 9, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 9, 34 -- s=s1024; d=yahoo.com; } 9, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; } 9, 34 -- b=UzFS8svM8Y/B+7qP/Q/c5DSECyhFf6TZA8lThkmYbw52LynZzAAzDAcKcUd23cgsoJmiRvhrts1AnmUtuian/x/HKHy/ERTRPbhILAAZuJXI9NtgXqykUyAuQlhcFqKcXQCVjWVC8hWxWPzLmcOEaZLi9zFPKNKy+QOsl5+QrZ0=; } 9, 34 -- Message-ID: {339537.10240.qm-at-web110805.mail.gq1.yahoo.com} } 9, 34 -- X-YMail-OSG: qaRsTmoVM1lvgJBqqtJ0oHlctXIXm8k_7L_6p.jD3ZSuqRq } 9, 34 -- 0Pr1PagNdTjzcFmqQBOU8PscC6c_svU4lkWxMTQM.5skIF12SqleEMSppADt } 9, 34 -- BpMoMSAonk2MLJ0Wd0MgE20anLCSmr.1Bz96r9SILsXGymxBF1JGiP6hHHnq } 9, 34 -- 6s6CR5m7PP5hexpEu2V7NKo6helkqNeMOnvtZmB4q5xEnKL7lmadpvBq8sLi } 9, 34 -- 3n1i9hewK9HtG4dW9lUC8VjGfyi8eEEXw1OcBmbkNNE_Mh26.UYcO9C_U.98 } 9, 34 -- _UJpzs6QKrWL70rCmir6M0oACKcTTzEQw8xZuDQ5Y3oW1B5NIDM87rFwhg0Y } 9, 34 -- wfyt9PhlW.O3J8mrKARhRr2bP9KougD8rdsLWM.h8qtO1m6EnQHZqaFfCwAV } 9, 34 -- DvlwYrxAXK400 } 9, 34 -- Received: from [80.122.101.100] by web110805.mail.gq1.yahoo.com via HTTP; Thu, 10 Feb 2011 05:16:39 PST } 9, 34 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.108.291010 } 9, 34 -- Date: Thu, 10 Feb 2011 05:16:39 -0800 (PST) } 9, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 9, 34 -- Subject: reference manager software } 9, 34 -- To: microscopy-at-microscopy.com } 9, 34 -- MIME-Version: 1.0 } 9, 34 -- Content-Type: text/plain; charset=iso-8859-1 } 9, 34 -- Content-Transfer-Encoding: 8bit } 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ADGeAg012338 } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both shervin.esfahani-at-nih.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Large Dense Core Vesicles
Message: Good morning everyone, I am currently trying to obtain good micrographs of LDCV's (large dense core vesicles) but, have had a variety of issues when I go image them. Ideally, the LDCV will have a distinct outer membrane, with a large, dense and darkly stained interior. There is also a thin space between the interior peptides and the membrane. (Hopefully this all makes sense so far) I have been having issues where the contents inside the LDCV's tend to shrink and shrivel up inside the membrane or, the entire LDCV is stained so dark that there is no discernable membrane, and it resembles a staining artifact. I have tried 4 different fixations (2.5% Glut in Sodium Cacodylate, 4% Glut in Sodium Cacodylate, 1% Glut & 1% OsO4 in Sodium Phosphate, and 2.5% Glut & 1% Paraformaldehyde in Sodium Phosphate), each for 1 hr, followed by 1% OsO4 (except in the case of the double fix), 1% Uranyl Acetate in cold/dark overnight, standard ethanol dehydration, embedding in EMbed-812, and finally post section staining with 1% Uranyl Acetate for 10 min (in dark), followed by Lead Citrate staining for 2-3 min. Just wondering if anyone has any tips on how to prevent this shrinking, or if there are any other tips on what works best on these LDCV's. Thanks in advance! Shervin G Esfahani NIH/IRTA Fellow NHLBI EM Core Facility 9000 Rockville Pike Bethesda, Maryland 20892 301.496.6448
Opinions and experiences related are those of Shervin Esfahani and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball Institute of Biomolecular Medicine
Title-Subject: [Filtered] Nikon TIRF question
Message: Dear microscopists,
We have an issue with our TIRF system that the illumination appears much br= ighter in the center of the field than the edges. Example: http://www.flickr.com/photos/mcammer/5436640624/ Does anybody have tips for aligning the Nikon to avoid this?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ludo.rossou-at-ua.ac.be as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ludo.rossou-at-ua.ac.be Name: Rossou ludo
Organization: university of Antwerp
Title-Subject: [Filtered] amourohous metal layer
Message: Dear all,
How to deposit a 100% amourphous metal layer without forming any crystals. Now I use therrmal evaporation by resitance heating but we still have crystals of the metal. Sputtering gives also to much crystals. Anybody a better idea or suggestion?
You didn't say what metal that you are trying to deposit. Regardless, I can't think of any metals that will go down amorphous. Carbon will. There are some metals that will go down with very small grains and they are used for sputter deposition for high resolution SEM imaging. Some examples are Cr, Ir, W.
There may be some alloys that can be processed to be amorphous, typically by rapid queching from a melt. There are some newer amorphous alloys that the rate can be relatively slow. I have no idea whether those alloys can be deposited as a thin film in the amorphous state or not. You will have to do a search for the alloy composition of some of these materials, but I believe that most of them are 3, if not 4, or more components.
Is there some reason that you need an amorphous film? Can a fine grain film such as Ir work for you?
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com SWalck-at-SouthBayTech.com
On Fri 11/02/11 4:52 PM , microscopylistserver-noreply-at-microscopy.com sent: } } } } --------------------------------------------------------------------------- } -The Microscopy ListServer -- CoSponsor: The Microscopy Society of } AmericaTo Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserverOn-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html--------------------------------------------------------------------------- } - } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html--------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both ludo.ros } sou-at-ua.ac.be as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ludo.ros } sou-at-ua.ac.be Name: Rossou ludo } Organization: university of Antwerp } } Title-Subject: [Filtered] amourohous metal layer } } Message: Dear all, } } How to deposit a 100% amourphous metal layer without forming any } crystals.Now I use therrmal evaporation by resitance heating but we still have } crystals of the metal. } Sputtering gives also to much crystals. } Anybody a better idea or suggestion? } } Thanks for your help! } } Regards, } Ludo } } ROSSOU Ludo } Universiteit Antwerpen } Dept. Fysica - EMAT } Groenenborgerlaan 171 } B-2020 Antwerpen } Belgium } ludo.ros } sou-at-ua.ac.bewww.emat.ua.ac.be } } } Login Host: 143.129.150.28 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers==============================14, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 11 } 18:43:44 201114, 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1C0hiDl02801414, 25 -- for roscopy-at-microscopy.com} ; Fri, 11 Feb 2011 18:43:44 -060014, 25 -- Received: from localhost (localhost [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id DDD4A32F9EE } 14, 25 -- for roscopy-at-microscopy.com} ; Fri, 11 Feb 2011 18:43:44 -0600 (CST)14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port 10024)14, 25 -- with ESMTP id 2GahqezTPbqo for roscopy-at-microscopy.com} ;14, 25 -- Fri, 11 Feb 2011 18:43:43 -0600 (CST) } 14, 25 -- Received: from NestorPwrBk.local (b4ef2.static.pacific.net.au } [125.255.46.242])14, 25 -- by znl.com (Postfix) with ESMTPA id 0627F32F9E3 } 14, 25 -- for roscopy-at-microscopy.com} ; Fri, 11 Feb 2011 18:43:42 -0600 (CST)14, 25 -- Message-ID: 5D7BD.9-at-microscopy.com} 14, 25 -- Date: Sat, 12 Feb 2011 11:43:41 +1100 } 14, 25 -- From: MicroscopyListserver-NoReply 14, 25 -- Reply-To: ludo.ros } sou-at-ua.ac.be14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.414, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward roscopy-at-microscopy.com} 14, 25 -- Subject: viaWWW:amourohous metal layer } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 13, 20 -- From swalck-at-southbaytech.com Fri Feb 11 20:10:35 2011 13, 20 -- Received: from www2.webmail.pair.com (www2.webmail.pair.com [66.39.3.96]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1C2AZMD025893 13, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Feb 2011 20:10:35 -0600 13, 20 -- Received: (qmail 45588 invoked from network); 12 Feb 2011 02:10:35 -0000 13, 20 -- Received: from localhost.pair.com (HELO localhost) (127.0.0.1) 13, 20 -- by localhost.pair.com with SMTP; 12 Feb 2011 02:10:35 -0000 13, 20 -- MIME-Version: 1.0 13, 20 -- X-Mailer: AtMail PHP 5.5 13, 20 -- Message-ID: {50382.1297476635-at-southbaytech.com} 13, 20 -- To: {Microscopy-at-microscopy.com} 13, 20 -- Reply-To: swalck-at-southbaytech.com 13, 20 -- Content-Type: text/plain; charset="utf-8" 13, 20 -- X-Origin: 69.198.103.186 13, 20 -- Date: Fri, 11 Feb 2011 21:10:35 -0500 13, 20 -- Subject: Re: [Microscopy] viaWWW:amourohous metal layer 13, 20 -- From: Scott Walck {swalck-at-southbaytech.com} 13, 20 -- Cc: {sou-at-ua.ac.be} 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1C2AZMD025893 ==============================End of - Headers==============================
You didn't say what metal that you are trying to deposit. Regardless, I can't think of any metals that will go down amorphous. Carbon will. There are some metals that will go down with very small grains and they are used for sputter deposition for high resolution SEM imaging. Some examples are Cr, Ir, W.
There may be some alloys that can be processed to be amorphous, typically by rapid queching from a melt. There are some newer amorphous alloys that the rate can be relatively slow. I have no idea whether those alloys can be deposited as a thin film in the amorphous state or not. You will have to do a search for the alloy composition of some of these materials, but I believe that most of them are 3, if not 4, or more components.
Is there some reason that you need an amorphous film? Can a fine grain film such as Ir work for you?
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com SWalck-at-SouthBayTech.com
On Fri 11/02/11 4:52 PM , microscopylistserver-noreply-at-microscopy.com sent: } } } } --------------------------------------------------------------------------- } -The Microscopy ListServer -- CoSponsor: The Microscopy Society of } AmericaTo Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserverOn-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html--------------------------------------------------------------------------- } - } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html--------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both ludo.ros } sou-at-ua.ac.be as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: ludo.ros } sou-at-ua.ac.be Name: Rossou ludo } Organization: university of Antwerp } } Title-Subject: [Filtered] amourohous metal layer } } Message: Dear all, } } How to deposit a 100% amourphous metal layer without forming any } crystals.Now I use therrmal evaporation by resitance heating but we still have } crystals of the metal. } Sputtering gives also to much crystals. } Anybody a better idea or suggestion? } } Thanks for your help! } } Regards, } Ludo } } ROSSOU Ludo } Universiteit Antwerpen } Dept. Fysica - EMAT } Groenenborgerlaan 171 } B-2020 Antwerpen } Belgium } ludo.ros } sou-at-ua.ac.bewww.emat.ua.ac.be } } } Login Host: 143.129.150.28 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers==============================14, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Feb 11 } 18:43:44 201114, 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1C0hiDl02801414, 25 -- for roscopy-at-microscopy.com} ; Fri, 11 Feb 2011 18:43:44 -060014, 25 -- Received: from localhost (localhost [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id DDD4A32F9EE } 14, 25 -- for roscopy-at-microscopy.com} ; Fri, 11 Feb 2011 18:43:44 -0600 (CST)14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port 10024)14, 25 -- with ESMTP id 2GahqezTPbqo for roscopy-at-microscopy.com} ;14, 25 -- Fri, 11 Feb 2011 18:43:43 -0600 (CST) } 14, 25 -- Received: from NestorPwrBk.local (b4ef2.static.pacific.net.au } [125.255.46.242])14, 25 -- by znl.com (Postfix) with ESMTPA id 0627F32F9E3 } 14, 25 -- for roscopy-at-microscopy.com} ; Fri, 11 Feb 2011 18:43:42 -0600 (CST)14, 25 -- Message-ID: 5D7BD.9-at-microscopy.com} 14, 25 -- Date: Sat, 12 Feb 2011 11:43:41 +1100 } 14, 25 -- From: MicroscopyListserver-NoReply 14, 25 -- Reply-To: ludo.ros } sou-at-ua.ac.be14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.414, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward roscopy-at-microscopy.com} 14, 25 -- Subject: viaWWW:amourohous metal layer } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 13, 20 -- From swalck-at-southbaytech.com Fri Feb 11 20:10:35 2011 13, 20 -- Received: from www2.webmail.pair.com (www2.webmail.pair.com [66.39.3.96]) 13, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1C2AZKw025892 13, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Feb 2011 20:10:35 -0600 13, 20 -- Received: (qmail 45586 invoked from network); 12 Feb 2011 02:10:35 -0000 13, 20 -- Received: from localhost.pair.com (HELO localhost) (127.0.0.1) 13, 20 -- by localhost.pair.com with SMTP; 12 Feb 2011 02:10:35 -0000 13, 20 -- MIME-Version: 1.0 13, 20 -- X-Mailer: AtMail PHP 5.5 13, 20 -- Message-ID: {50387.1297476635-at-southbaytech.com} 13, 20 -- To: {Microscopy-at-microscopy.com} 13, 20 -- Reply-To: swalck-at-southbaytech.com 13, 20 -- Content-Type: text/plain; charset="utf-8" 13, 20 -- X-Origin: 69.198.103.186 13, 20 -- Date: Fri, 11 Feb 2011 21:10:35 -0500 13, 20 -- Subject: Re: [Microscopy] viaWWW:amourohous metal layer 13, 20 -- From: Scott Walck {swalck-at-southbaytech.com} 13, 20 -- Cc: {sou-at-ua.ac.be} 13, 20 -- Content-Transfer-Encoding: 8bit 13, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1C2AZKw025892 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both martin.saunders-at-uwa.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: martin.saunders-at-uwa.edu.au Name: Martin Saunders
Organization: The University of Western Australia
Title-Subject: [Filtered] Job opening: SEM academic (Assistant Prof/Associate Prof)
Message: The Centre for Microscopy, Characterisaton and Analysis at The University of Western Australia is seeking suitable applicants for an academic position (Assistant Prof/Associate Prof) to lead our scanning electron microscopy capability. The CMCA is a major central university facility that supports the research-intensive role of the university, forming a nexus for collaborative and interdisciplinary research linked to applications of our extensive microscopy and microanalysis capabilities, which include SEM, TEM, EPMA, ion probes, optical, confocal, flow cytometry, cell sorting, XRD, NMR and mass spec.
The successful candidate will have a background in the application of SEM in either the physical or biological sciences. They will be expected to lead SEM-related activity in the Centre, including the provision of teaching/training, developing their own research programs, establishing collaborative and interdisciplinary research activities with local researchers, and driving future plans for SEM capabilities at the university.
More information on this position can be found at https://www.his.admin.uwa.edu.au/jobvacs/external/academic/record/record1926699.html
Queries can be directed to our Centre Director (Prof David Sampson, david.sampson-at-uwa.edu.au) or myself (martin.saunders-at-uwa.edu.au).
Regards,
Martin.
*****************************************
Professor Martin Saunders, Deputy Director, Centre for Microscopy, Characterisation and Analysis, M010, The University of Western Australia, Crawley, Western Australia 6009, Australia.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both info-at-tescan-usa.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job Opening: TESCAN District Sales Manager, Western Region
Message: TESCAN, a leading manufacturer of Scanning Electron Microscopes and Focused Ion Beam Workstations established in 1991 is accepting resumes for a District Sales Manager in the Silicon Valley California region of the United States. The person chosen should be self-motivated, independent and a team player. A technical degree and background is required, experience with charge particle technology would be ideal. Candidates should possess excellent communication and organizational skills. All interested parties please submit resumes and salary requirements to info-at-tescan-usa.com. Previous sales experience is preferred but not necessarily required. Qualified candidates must be able to pass criminal, and credit background checks, including drug testing. A valid drivers License is required. TESCAN is an affirmative action/equal opportunity employer. Please limit all correspondence to info-at-tescan-usa.com.
Hi Ludo, What metal do you want to deposit? Whether you can get an amorphous layer depends on the metal, and in many cases you can only get crystals, although sometimes these can be nm-sized. As I remember, one can get an amorphous layer of tungsten, but I may have that wrong. If so, I hope someone else on the list will correct me. Since the crystal structure usually has the lowest energy, it is difficult to get something non-crystalline, but possibly there are alloys that do not form crystals. Again, I hope someone else from the list will know. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
How to deposit a 100% amourphous metal layer without forming any crystals. Now I use therrmal evaporation by resitance heating but we still have crystals of the metal. Sputtering gives also to much crystals. Anybody a better idea or suggestion?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1EKjJfP006250 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 14 Feb 2011 14:45:19 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p1EKjJjb006248; Mon, 14 Feb 2011 14:45:19 -0600
Colleagues,
Because of problems we have been experiencing with network congestion, the deadline for paper submission for MM2011 has been extended to Monday Feb 21st 11:59 PM EST.
The direct link to paper submission site is:
http://mam2011.abstractcentral.com/index.jsp
and for general information about the meeting
http://microscopy.org/MandM/2011/index.cfm
On behalf of the Society please accept my appology for the delays and associated problems.
Nestor J. Zaluzec President Microscopy Society of America --
==============================Original Headers============================== 8, 25 -- From zaluzec-at-aaem.amc.anl.gov Mon Feb 14 14:45:18 2011 8, 25 -- Received: from znl.com ([206.69.208.20]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1EKjIgT006245 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 14:45:18 -0600 8, 25 -- Received: from localhost (localhost [127.0.0.1]) 8, 25 -- by znl.com (Postfix) with ESMTP id D09C83333AA 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 14:45:18 -0600 (CST) 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 8, 25 -- Received: from znl.com ([127.0.0.1]) 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 8, 25 -- with ESMTP id PCx1qTc78NCA for {microscopy-at-microscopy.com} ; 8, 25 -- Mon, 14 Feb 2011 14:45:18 -0600 (CST) 8, 25 -- Received: from NestorPwrBk.local (b4ef2.static.pacific.net.au [125.255.46.242]) 8, 25 -- by znl.com (Postfix) with ESMTPA id E65B233339F 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 14:45:17 -0600 (CST) 8, 25 -- Message-ID: {4D59945C.1050607-at-aaem.amc.anl.gov} 8, 25 -- Date: Tue, 15 Feb 2011 07:45:16 +1100 8, 25 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 8, 25 -- Organization: ANL 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 8, 25 -- MIME-Version: 1.0 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 8, 25 -- Subject: MM2011 Paper Submission Deadline Extended 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
So much 'Darpa' in the world. First the bomb, then the internet, now strange and amorphous metal coatings.
Cheers,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Monday, February 14, 2011 12:39 PM To: Monson, Frederick
Hi Ludo, What metal do you want to deposit? Whether you can get an amorphous layer depends on the metal, and in many cases you can only get crystals, although sometimes these can be nm-sized. As I remember, one can get an amorphous layer of tungsten, but I may have that wrong. If so, I hope someone else on the list will correct me. Since the crystal structure usually has the lowest energy, it is difficult to get something non-crystalline, but possibly there are alloys that do not form crystals. Again, I hope someone else from the list will know. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
How to deposit a 100% amourphous metal layer without forming any crystals. Now I use therrmal evaporation by resitance heating but we still have crystals of the metal. Sputtering gives also to much crystals. Anybody a better idea or suggestion?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ENCi0Z010154 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 14 Feb 2011 17:12:44 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p1ENCikN010152; Mon, 14 Feb 2011 17:12:44 -0600
Most every high capacity disk drive made today, which probably includes the one in the computer you are using right now, has one or more layers of amorphous, magnetically soft material called "soft under layer". I cannot disclose any detail about this material, but if you just Google "soft under layer" or "soft under layer perpendicular recording" you can learn quite a bit from public sources.
John Mardinly
Western Digital
-----Original Message----- X-from: FMonson-at-wcupa.edu [mailto:FMonson-at-wcupa.edu] Sent: Monday, February 14, 2011 1:44 PM To: John Mardinly
So much 'Darpa' in the world. First the bomb, then the internet, now strange and amorphous metal coatings.
Cheers,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Monday, February 14, 2011 12:39 PM To: Monson, Frederick
Hi Ludo, What metal do you want to deposit? Whether you can get an amorphous layer depends on the metal, and in many cases you can only get crystals, although sometimes these can be nm-sized. As I remember, one can get an amorphous layer of tungsten, but I may have that wrong. If so, I hope someone else on the list will correct me. Since the crystal structure usually has the lowest energy, it is difficult to get something non-crystalline, but possibly there are alloys that do not form crystals. Again, I hope someone else from the list will know. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
How to deposit a 100% amourphous metal layer without forming any crystals. Now I use therrmal evaporation by resitance heating but we still have crystals of the metal. Sputtering gives also to much crystals. Anybody a better idea or suggestion?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ENYCGE026547 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 14 Feb 2011 17:34:12 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p1ENYCIL026545; Mon, 14 Feb 2011 17:34:12 -0600
Ludo-
You could consider Osmium Coating (http://www.2spi.com/catalog/osmi-coat.html)...again, the utility depends on what the application is. The literature suggests this coating is completely amorphous, but I haven't verified this myself. However, the coating is extremely conformal and smooth - we use it routinely for high-res SEM and it is difficult to see any texture, whereas we can easily see grains in fine grain Au/Pd or Pt films.
Regards, Ben Myers NUANCE Center - Northwestern University
On 2/14/2011 5:21 PM, John.Mardinly-at-wdc.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Most every high capacity disk drive made today, which probably includes the } one in the computer you are using right now, has one or more layers of } amorphous, magnetically soft material called "soft under layer". I cannot } disclose any detail about this material, but if you just Google "soft under } layer" or "soft under layer perpendicular recording" you can learn quite a } bit from public sources. } } John Mardinly } } Western Digital } } -----Original Message----- } X-from: FMonson-at-wcupa.edu [mailto:FMonson-at-wcupa.edu] } Sent: Monday, February 14, 2011 1:44 PM } To: John Mardinly } Subject: [Microscopy] viaWWW:amourohous metal layer } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } http://www.darpa.mil/dso/thrusts/materials/novelmat/naval/index.htm } } So much 'Darpa' in the world. First the bomb, then the internet, now } strange and amorphous metal coatings. } } Cheers, } } Fred Monson } } Frederick C. Monson, PhD } Technical Director } Center for Microanalysis and Imaging, Research and Training (CMIRT) } Schmucker Science Center South } West Chester University, West Chester, PA 19383 } 610-738-0437 } } Home Page: http://cmirt.wcupa.edu } Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl } } } -----Original Message----- } X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] } Sent: Monday, February 14, 2011 12:39 PM } To: Monson, Frederick } Subject: [Microscopy] Re: viaWWW:amourohous metal layer } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Ludo, } What metal do you want to deposit? Whether you can get an } amorphous layer depends on the metal, and in many cases you can only get } crystals, although sometimes these can be nm-sized. As I remember, one } can get an amorphous layer of tungsten, but I may have that wrong. If so, } I hope someone else on the list will correct me. Since the crystal } structure usually has the lowest energy, it is difficult to get something } non-crystalline, but possibly there are alloys that do not form crystals. } Again, I hope someone else from the list will know. } Yours, } Bill } } } } X-from: microscopylistserver-noreply-at-microscopy.com } To: William.F.Tivol-at-aero.org } Date: 02/11/2011 04:54 PM } Subject: [Microscopy] viaWWW:amourohous metal layer } } } How to deposit a 100% amourphous metal layer without forming any crystals. } Now I use therrmal evaporation by resitance heating but we still have } crystals of the metal. } Sputtering gives also to much crystals. } Anybody a better idea or suggestion? } } Thanks for your help! } } Regards, } Ludo } } } ==============================Original Headers============================== } 9, 48 -- From prvs=019dab85a=William.F.Tivol-at-aero.org Mon Feb 14 11:29:01 } 2011 } 9, 48 -- Received: from email1-west.aero.org (email1-west.aero.org } [130.221.16.26]) } 9, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1EHT05d014744 } 9, 48 -- for {microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 11:29:00 } -0600 } 9, 48 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 9, 48 -- d=aero.org; i=William.F.Tivol-at-aero.org; q=dns/txt; } 9, 48 -- s=mailhub; t=1297704541; x=1329240541; } 9, 48 -- h=in-reply-to:references:to:cc:mime-version:subject: } 9, 48 -- message-id:date:from; } 9, 48 -- z=In-Reply-To:=20 {201102120054.p1C0sONK022936-at-ns.microscop } 9, 48 -- y.com} |References:=20 {201102120054.p1C0sONK022936-at-ns.micr } 9, 48 -- oscopy.com} |To:=20microscopy-at-microscopy.com|Cc:=20ludo.ro } 9, 48 -- ssou-at-ua.ac.be|MIME-Version:=201.0|Subject:=20Re:=20[Micro } 9, 48 -- scopy]=20viaWWW:amourohous=20metal=20layer|Message-ID:=20 } 9, 48 -- {OFB5D709B3.C1304B4B-ON88257837.005F3FAA-88257837.00600A7 } 9, 48 -- 0-at-notes.aero.org} |Date:=20Mon,=2014=20Feb=202011=2009:28: } 9, 48 -- 59=20-0800|From:=20William=20F=20Tivol=20 {William.F.Tivol } 9, 48 -- -at-aero.org} ; } 9, 48 -- bh=txaPeOLJne4bmEgVq1lVxGiyRI+fFQZXQZZ/jAEGyVc=; } 9, 48 -- b=nYUtF5DAbFHxb6KUVBTDNKe2St0EFqoACD8Uyc7qKZMmsAVEfrSsPKgj } 9, 48 -- C0/O26vplHyUTfMTElzcEyTn17J3pCq/92BeScR72fdWRWE6q7qHnQ0NC } 9, 48 -- 6TtHD5EP9Ig6lpvimf/WRgYeyOO4QkHK0e6CIKtETa218Xh/gnFITqxPT } 9, 48 -- I=; } 9, 48 -- From: William F Tivol {William.F.Tivol-at-aero.org} } 9, 48 -- x-SBRS: 2.9 } 9, 48 -- x-SenderGroup: Internal_Relay_hosts } 9, 48 -- X-IronPort-Anti-Spam-Filtered: true } 9, 48 -- X-IronPort-Anti-Spam-Result: } AlkGAPT0WE2C3VwSgWdsb2JhbACXbY4eFQEBFiIkuzWFXgSFBIZ7 } 9, 48 -- X-IronPort-AV: E=McAfee;i="5400,1158,6257"; a="50531313" } 9, 48 -- X-IronPort-AV: E=Sophos;i="4.60,469,1291622400"; } 9, 48 -- d="scan'208";a="50531313" } 9, 48 -- Received: from agosmtpout.aero.org ([130.221.92.18]) } 9, 48 -- by mailhub1.aero.org with ESMTP; 14 Feb 2011 09:29:00 -0800 } 9, 48 -- In-Reply-To: {201102120054.p1C0sONK022936-at-ns.microscopy.com} } 9, 48 -- References: {201102120054.p1C0sONK022936-at-ns.microscopy.com} } 9, 48 -- To: microscopy-at-microscopy.com } 9, 48 -- Cc: ludo.rossou-at-ua.ac.be } 9, 48 -- MIME-Version: 1.0 } 9, 48 -- Subject: Re: [Microscopy] viaWWW:amourohous metal layer } 9, 48 -- X-KeepSent: B5D709B3:C1304B4B-88257837:005F3FAA; } 9, 48 -- type=4; name=$KeepSent } 9, 48 -- X-Mailer: Lotus Notes Release 8.5.1 September 28, 2009 } 9, 48 -- Message-ID: } {OFB5D709B3.C1304B4B-ON88257837.005F3FAA-88257837.00600A70-at-notes.aero.org} } 9, 48 -- Date: Mon, 14 Feb 2011 09:28:59 -0800 } 9, 48 -- X-MIMETrack: Serialize by Router on } agosmtpout/AeroNet/Aerospace/US(Release 8.5.2 HF250|November } 9, 48 -- 18, 2010) at 02/14/2011 09:29:00 AM, } 9, 48 -- Serialize complete at 02/14/2011 09:29:00 AM } 9, 48 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 22, 37 -- From FMonson-at-wcupa.edu Mon Feb 14 15:36:53 2011 } 22, 37 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) } 22, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1ELarI6023797 } 22, 37 -- for {microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 15:36:53 } -0600 } 22, 37 -- X-ASG-Debug-ID: 1297719412-0368bc2a5884bd0001-4CH8be } 22, 37 -- Received: from WCU-XCH-08.PASSHE.LCL (wcu-xch-08.wcupa.edu } [144.26.0.92]) by MX01.WCUPA.EDU with ESMTP id g0S0YE3CDPCyDLcq } (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Mon, 14 Feb 2011 } 16:36:52 -0500 (EST) } 22, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu } 22, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.92 } 22, 37 -- X-ASG-Whitelist: Client } 22, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) } by } 22, 37 -- WCU-XCH-08.PASSHE.LCL ([fe80::7048:475f:4ea9:edce%15]) with mapi } id } 22, 37 -- 14.01.0270.001; Mon, 14 Feb 2011 16:36:52 -0500 } 22, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} } 22, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 22, 37 -- CC: "William.F.Tivol-at-aero.org" {William.F.Tivol-at-aero.org} } 22, 37 -- Subject: RE: [Microscopy] Re: viaWWW:amourohous metal layer } 22, 37 -- Thread-Topic: [Microscopy] Re: viaWWW:amourohous metal layer } 22, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: viaWWW:amourohous metal } layer } 22, 37 -- Thread-Index: AQHLzG4H3ccHR2okc0CGa+p8Ry0N/5QBRZpQ } 22, 37 -- Date: Mon, 14 Feb 2011 21:36:51 +0000 } 22, 37 -- Message-ID: } {899EAA40AE6E47488CE0637489EFB30C021CF8-at-WCU-XCH-03.PASSHE.LCL} } 22, 37 -- References: {201102141738.p1EHcgkR027788-at-ns.microscopy.com} } 22, 37 -- In-Reply-To: {201102141738.p1EHcgkR027788-at-ns.microscopy.com} } 22, 37 -- Accept-Language: en-US } 22, 37 -- Content-Language: en-US } 22, 37 -- X-MS-Has-Attach: } 22, 37 -- X-MS-TNEF-Correlator: } 22, 37 -- x-originating-ip: [10.28.57.79] } 22, 37 -- Content-Type: text/plain; charset="us-ascii" } 22, 37 -- MIME-Version: 1.0 } 22, 37 -- X-Barracuda-Connect: wcu-xch-08.wcupa.edu[144.26.0.92] } 22, 37 -- X-Barracuda-Start-Time: 1297719412 } 22, 37 -- X-Barracuda-Encrypted: AES128-SHA } 22, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi } 22, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU } 22, 37 -- Content-Transfer-Encoding: 8bit } 22, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p1ELarI6023797 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 31, 30 -- From prvs=0195811d7=John.Mardinly-at-wdc.com Mon Feb 14 17:12:44 2011 } 31, 30 -- Received: from wdscspam2.wdc.com (wdscspam2.wdc.com [129.253.55.43]) } 31, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ENChej010149 } 31, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 17:12:43 -0600 } 31, 30 -- X-IronPort-AV: E=Sophos;i="4.60,470,1291622400"; } 31, 30 -- d="scan'208";a="173829179" } 31, 30 -- Received: from unknown (HELO wdscexfe01.sc.wdc.com) ([10.4.40.191]) } 31, 30 -- by wdscspam2.wdc.com with ESMTP; 14 Feb 2011 15:12:36 -0800 } 31, 30 -- Received: from wdksjexbe01.msj.wdc.com ([172.19.100.67]) by wdscexfe01.sc.wdc.com with Microsoft SMTPSVC(6.0.3790.4675); } 31, 30 -- Mon, 14 Feb 2011 15:12:43 -0800 } 31, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 31, 30 -- Content-class: urn:content-classes:message } 31, 30 -- MIME-Version: 1.0 } 31, 30 -- Content-Type: text/plain; } 31, 30 -- charset="us-ascii" } 31, 30 -- Subject: RE: [Microscopy] viaWWW:amourohous metal layer } 31, 30 -- Date: Mon, 14 Feb 2011 15:12:42 -0800 } 31, 30 -- Message-ID: {34061960C62E274C8AF1DCAA65655558097AA729-at-wdksjexbe01.msj.wdc.com} } 31, 30 -- In-Reply-To: {201102142143.p1ELhWBZ004088-at-ns.microscopy.com} } 31, 30 -- X-MS-Has-Attach: } 31, 30 -- X-MS-TNEF-Correlator: } 31, 30 -- Thread-Topic: [Microscopy] viaWWW:amourohous metal layer } 31, 30 -- Thread-Index: AcvMkDr82PGA93fMRlqy1yrqyNdlHQAC43eA } 31, 30 -- References: {201102142143.p1ELhWBZ004088-at-ns.microscopy.com} } 31, 30 -- From: "John Mardinly" {John.Mardinly-at-wdc.com} } 31, 30 -- To: {FMonson-at-wcupa.edu} } 31, 30 -- Cc: {Microscopy-at-microscopy.com} } 31, 30 -- X-OriginalArrivalTime: 14 Feb 2011 23:12:43.0251 (UTC) FILETIME=[AEF5F430:01CBCC9C] } 31, 30 -- Content-Transfer-Encoding: 8bit } 31, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1ENChej010149 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 18 -- From b-myers3-at-northwestern.edu Mon Feb 14 17:34:12 2011 5, 18 -- Received: from mailp5.ci.northwestern.edu (lulu.it.northwestern.edu [129.105.16.95]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ENYCkZ026542 5, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 17:34:12 -0600 5, 18 -- Received: from [129.105.37.199] (ms199.ms.northwestern.edu [129.105.37.199]) 5, 18 -- by mailp5.ci.northwestern.edu (Postfix) with ESMTP id 63CA576DE6 5, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 17:34:12 -0600 (CST) 5, 18 -- Message-ID: {4D59BBF1.2000207-at-northwestern.edu} 5, 18 -- Date: Mon, 14 Feb 2011 17:34:09 -0600 5, 18 -- From: Ben Myers {b-myers3-at-northwestern.edu} 5, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 5, 18 -- MIME-Version: 1.0 5, 18 -- To: Microscopy-at-microscopy.com 5, 18 -- Subject: RE: viaWWW:amourohous metal layer 5, 18 -- References: {201102142321.p1ENL4Ff024150-at-ns.microscopy.com} 5, 18 -- In-Reply-To: {201102142321.p1ENL4Ff024150-at-ns.microscopy.com} 5, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We are interested in purchasing a grid coating pen to save time but we still want a stable coating under the beam. Does anyone has previous experience using one of those pens ? Is it useful or just a gadget?
Thanks again!
Tami
-- Tami Bogea PhD Research Associate Ophthalmology & Visual Sciences University of British Columbia 2550 Willow St. Vancouver, BC V5Z 3N9 Canada
==============================Original Headers============================== 6, 27 -- From tbogea-at-interchange.ubc.ca Mon Feb 14 17:41:49 2011 6, 27 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ENfmUZ010003 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 17:41:49 -0600 6, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 6, 27 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP id 2C71316C6F 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 15:41:48 -0800 (PST) 6, 27 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12]) 6, 27 -- by smtp.interchange.ubc.ca 6, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 6, 27 -- with ESMTP id {0LGM009H4SHME6-at-smtp.interchange.ubc.ca} for 6, 27 -- Microscopy-at-microscopy.com; Mon, 14 Feb 2011 15:41:47 -0800 (PST) 6, 27 -- Date: Mon, 14 Feb 2011 15:41:46 -0800 (PST) 6, 27 -- From: Tami Bogea {tbogea-at-interchange.ubc.ca} 6, 27 -- Subject: Grid coating pen for TEM 6, 27 -- To: Microscopy-at-microscopy.com 6, 27 -- Message-id: {30290911.4201297726906935.JavaMail.myubc2-at-brahms.my.ubc.ca} 6, 27 -- MIME-version: 1.0 6, 27 -- X-Mailer: uPortal WEB email client 3.0 6, 27 -- Content-type: text/plain; charset=us-ascii 6, 27 -- Content-transfer-encoding: 7bit 6, 27 -- X-UBC-Scanned: Sophos PureMessage 5.5.9.388399, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.2.14.233021 6, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 6, 27 -- X-PerlMx-Spam: Probability=8%, Report= 6, 27 -- SUPERLONG_LINE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_400_499 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __ADULT_SUBJ_LO_2 0, __C230066_P1_5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0 6, 27 -- X-Spam-Level: 6, 27 -- X-Spam-Flag: No ==============================End of - Headers==============================
I've used them for about 15 years now. They're great. I buy them from Electron Microscopy Sciences. I coat 4-5 grids, a drop per grid, then move the grids from the drops while they are still wet to a dry spot on my filter paper, let the grids air dry for a minute, and they are ready to use. I only coat as many as I use, and don't coat them until I'm ready to use them. I pick up my sections by coming up from under the water with my grids. I never loose sections, and the sections are always flat and wrinkle-free. The only dirt I get is if I have dirt in my boat on top of the water.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: tbogea-at-interchange.ubc.ca [tbogea-at-interchange.ubc.ca] Sent: Monday, February 14, 2011 6:46 PM To: Haller, Edward
Hi all!
We are interested in purchasing a grid coating pen to save time but we still want a stable coating under the beam. Does anyone has previous experience using one of those pens ? Is it useful or just a gadget?
Thanks again!
Tami
-- Tami Bogea PhD Research Associate Ophthalmology & Visual Sciences University of British Columbia 2550 Willow St. Vancouver, BC V5Z 3N9 Canada
==============================Original Headers============================== 6, 27 -- From tbogea-at-interchange.ubc.ca Mon Feb 14 17:41:49 2011 6, 27 -- Received: from mr6.mail-relay.ubc.ca (mr6.mail-relay.ubc.ca [137.82.45.11]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1ENfmUZ010003 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 17:41:49 -0600 6, 27 -- Received: from mta2.interchange.ubc.ca (mta2.interchange.ubc.ca [142.103.145.70]) 6, 27 -- by mr6.mail-relay.ubc.ca (Postfix) with ESMTP id 2C71316C6F 6, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Feb 2011 15:41:48 -0800 (PST) 6, 27 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12]) 6, 27 -- by smtp.interchange.ubc.ca 6, 27 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 6, 27 -- with ESMTP id {0LGM009H4SHME6-at-smtp.interchange.ubc.ca} for 6, 27 -- Microscopy-at-microscopy.com; Mon, 14 Feb 2011 15:41:47 -0800 (PST) 6, 27 -- Date: Mon, 14 Feb 2011 15:41:46 -0800 (PST) 6, 27 -- From: Tami Bogea {tbogea-at-interchange.ubc.ca} 6, 27 -- Subject: Grid coating pen for TEM 6, 27 -- To: Microscopy-at-microscopy.com 6, 27 -- Message-id: {30290911.4201297726906935.JavaMail.myubc2-at-brahms.my.ubc.ca} 6, 27 -- MIME-version: 1.0 6, 27 -- X-Mailer: uPortal WEB email client 3.0 6, 27 -- Content-type: text/plain; charset=us-ascii 6, 27 -- Content-transfer-encoding: 7bit 6, 27 -- X-UBC-Scanned: Sophos PureMessage 5.5.9.388399, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.2.14.233021 6, 27 -- X-UBC-Relayed: Relayed through mail-relay.ubc.ca 6, 27 -- X-PerlMx-Spam: Probability=8%, Report= 6, 27 -- SUPERLONG_LINE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_400_499 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, WEBMAIL_SOURCE 0, WEBMAIL_XMAILER 0, __ADULT_SUBJ_LO_2 0, __C230066_P1_5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __PHISH_SPEAR_STRUCTURE_1 0, __PHISH_SPEAR_STRUCTURE_2 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0 6, 27 -- X-Spam-Level: 6, 27 -- X-Spam-Flag: No ==============================End of - Headers==============================
==============================Original Headers============================== 13, 37 -- From ehaller-at-health.usf.edu Tue Feb 15 07:42:51 2011 13, 37 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 13, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1FDgpC6017818 13, 37 -- for {microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 07:42:51 -0600 13, 37 -- X-ASG-Debug-ID: 1297777366-6a11028d0007-4CH8be 13, 37 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-bin/mark.cgi 13, 37 -- Received: from mailhub2.hscnet.hsc.usf.edu (localhost [127.0.0.1]) 13, 37 -- by hscantispam.health.usf.edu (Spam & Virus Firewall) with ESMTP 13, 37 -- id 07F6E7E9512F; Tue, 15 Feb 2011 08:42:47 -0500 (EST) 13, 37 -- Received: from mailhub2.hscnet.hsc.usf.edu ([10.119.4.13]) by hscantispam.health.usf.edu with ESMTP id FM1iIVmmjFz8K8z2; Tue, 15 Feb 2011 08:42:47 -0500 (EST) 13, 37 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 13, 37 -- Received: from MAILSERVER3.hscnet.hsc.usf.edu ([10.119.4.19]) by 13, 37 -- mailhub2.hscnet.hsc.usf.edu ([10.119.4.13]) with mapi; Tue, 15 Feb 2011 13, 37 -- 08:41:15 -0500 13, 37 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 13, 37 -- To: "tbogea-at-interchange.ubc.ca" {tbogea-at-interchange.ubc.ca} , 13, 37 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 13, 37 -- Date: Tue, 15 Feb 2011 08:41:15 -0500 13, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Grid coating pen for TEM 13, 37 -- Subject: RE: [Microscopy] Grid coating pen for TEM 13, 37 -- Thread-Topic: [Microscopy] Grid coating pen for TEM 13, 37 -- Thread-Index: AcvMoXaU5kzqHIb2TzuNtxbcwMdsYwAc8CN4 13, 37 -- Message-ID: {5B34016F48340D428A2644D479F587FC359C34F1-at-MAILSERVER3.hscnet.hsc.usf.edu} 13, 37 -- References: {201102142346.p1ENkt33022024-at-ns.microscopy.com} 13, 37 -- In-Reply-To: {201102142346.p1ENkt33022024-at-ns.microscopy.com} 13, 37 -- Accept-Language: en-US 13, 37 -- Content-Language: en-US 13, 37 -- X-MS-Has-Attach: 13, 37 -- X-MS-TNEF-Correlator: 13, 37 -- acceptlanguage: en-US 13, 37 -- Content-Type: text/plain; charset="us-ascii" 13, 37 -- MIME-Version: 1.0 13, 37 -- X-Barracuda-Connect: UNKNOWN[10.119.4.13] 13, 37 -- X-Barracuda-Start-Time: 1297777368 13, 37 -- X-Barracuda-Virus-Scanned: by USF Health AntiSpam System at health.usf.edu 13, 37 -- Content-Transfer-Encoding: 8bit 13, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1FDgpC6017818 ==============================End of - Headers==============================
Duke has an opening for an EM Technician. If you have any general questions you can ask me.
Otherwise, please follow the directions embedded in Dr. Miller's note.
Read the description below, if interested apply at: http://www.hr.duke.edu/jobs/
Thanks,
Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
NOTE: DO NOT REPLY TO THIS EMAIL. SEE BELOW FOR APPLICATION ADDRESS.
Electron Microscopy Position Available Electron Microscopy Technician, Senior
Location: Duke University Medical Center, Durham, NC
Requirements: Training and experience in running and maintaining electron microscopes, proficiency in cutting ultrathin sections and performing negative staining. Knowledge of scientific laboratory operation (making solutions, ordering, typing results, keeping records, etc.). Computer skills and research experience are a plus.
Laboratory description: The work force consists of the director and 6 EM technologists who perform pathology (500 samples/year), virology (1000 samples/year), and research. Instruments include s3 TEMs, 1 SEM, 7 ultramicrotomes—2 with cryo attachments, plus ancillary specimen preparation equipment.
Job descriptions available at: https://www.hr.duke.edu/ Click “Jobs” at the top left Under “Jobs Descriptions” click “University Job Descriptions” Under “Browse by Job Title” click “E” Then click “ELECTRON MICROSCOPY TECH, SR (0291)”
These descriptions are generic, and not all jobs described within (e.g., histology) are required of the EM position.
EM Laboratory web site: http://pathology.mc.duke.edu/website/WebForm.aspx?id=ElectronMicroMain
Send resume to: Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Laboratory P. O. Box 3712 Duke University Medical Center Durham, NC 27710
Has anyone removed the objective nosepiece from an Axioplan 2ie MOT? I think that ours stopped moving due to lubrication issues and would like to verify before sending a PO for potential parts replacements.
Thanks, Glen
Glen MacDonald Core for Communication Research Virginia Merrill Bloedel Hearing Research Center Box 357923 University of Washington Seattle, WA 98195-7923 USA (206) 616-4156 glenmac-at-uw.edu
==============================Original Headers============================== 13, 26 -- From glenmac-at-u.washington.edu Tue Feb 15 12:19:25 2011 13, 26 -- Received: from mxout14.cac.washington.edu (mxout14.cac.washington.edu [140.142.33.32]) 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1FIJPaG027827 13, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 12:19:25 -0600 13, 26 -- Received: from smtp.washington.edu (smtp.washington.edu [140.142.32.205] (may be forged)) 13, 26 -- by mxout14.cac.washington.edu (8.14.3+UW09.11/8.14.3+UW10.12) with ESMTP id p1FIIL9x025642 13, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 13, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 10:18:21 -0800 13, 26 -- X-Auth-Received: from d-128-95-178-200.dhcp4.washington.edu (D-128-95-178-200.dhcp4.washington.edu [128.95.178.200]) 13, 26 -- (authenticated authid=glenmac) 13, 26 -- by smtp.washington.edu (8.14.3+UW09.11/8.14.3+UW10.12) with ESMTP id p1FIIL9Y019438 13, 26 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT) 13, 26 -- for {microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 10:18:21 -0800 13, 26 -- From: Glen MacDonald {glenmac-at-u.washington.edu} 13, 26 -- Content-Type: text/plain; charset=us-ascii 13, 26 -- Subject: removing Axioplan nosepiece 13, 26 -- Date: Tue, 15 Feb 2011 10:18:21 -0800 13, 26 -- Message-Id: {5604F309-7A93-4F0C-AC4E-54B4ADF55EF6-at-u.washington.edu} 13, 26 -- To: "ListServer-at-MSA.Microscopy.Com Listserver" {microscopy-at-microscopy.com} 13, 26 -- Mime-Version: 1.0 (Apple Message framework v1082) 13, 26 -- X-Mailer: Apple Mail (2.1082) 13, 26 -- X-PMX-Version: 5.5.9.388399, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.2.15.180616 13, 26 -- X-Uwash-Spam: Gauge=IIIIIIII, Probability=8%, Report=' 13, 26 -- SUPERLONG_LINE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_400_499 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, __C230066_P5 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __MIME_VERSION_APPLEMAIL 0, __MSGID_APPLEMAIL 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NO_PATH 0, __URI_NO_WWW 0, __URI_NS , __USER_AGENT_APPLEMAIL 0, __X_MAILER_APPLEMAIL 0' 13, 26 -- Content-Transfer-Encoding: 8bit 13, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1FIJPaG027827 ==============================End of - Headers==============================
I am looking for a specimen holder for the Ivan Sorvall Porter-Blum MT-1 ultramicrotome. I need either the ball swivel collet or the MT2-to-MT1 adapter.
Thanks, Casey Peto
Salk Institute
==============================Original Headers============================== 4, 24 -- From peto-at-salk.edu Tue Feb 15 12:29:00 2011 4, 24 -- Received: from helix.salk.edu (helix.salk.edu [198.202.68.20]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1FISxM8008575 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 12:28:59 -0600 4, 24 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 4, 24 -- by helix.salk.edu (Postfix) with ESMTP id 41FBC240028 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 10:28:59 -0800 (PST) 4, 24 -- Received: from helix.salk.edu ([127.0.0.1]) 4, 24 -- by localhost (helix.salk.edu [127.0.0.1]) (amavisd-new, port 10024) 4, 24 -- with ESMTP id 30669-01 for {Microscopy-at-microscopy.com} ; 4, 24 -- Tue, 15 Feb 2011 10:28:59 -0800 (PST) 4, 24 -- Received: from [10.1.50.183] (unknown [10.1.50.183]) 4, 24 -- by helix.salk.edu (Postfix) with ESMTP id 1A5C5240002 4, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 10:28:59 -0800 (PST) 4, 24 -- Mime-Version: 1.0 (Apple Message framework v753.1) 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- Message-Id: {19BBEF13-2CC5-4A9B-A999-11D8C025DAE7-at-salk.edu} 4, 24 -- Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed 4, 24 -- To: Microscopy-at-microscopy.com 4, 24 -- From: C Peto {peto-at-salk.edu} 4, 24 -- Subject: TEM - request for MT-1 microtome chuck 4, 24 -- Date: Tue, 15 Feb 2011 10:29:06 -0800 4, 24 -- X-Mailer: Apple Mail (2.753.1) 4, 24 -- X-Virus-Scanned: amavisd-new at salk.edu ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both CGoldsmith-at-cdc.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Annual meeting of the Southeastern Microscopy Society
Message: The annual meeting of the Southeastern Microscopy Society (SEMS) will be held May 18-20 in Decatur, Georgia, just east of Atlanta. We will have a day and a half of presentations, and this year we are privileged to have as invited speakers Drs. Paul Hvala, Daniela Nicastro, Amelia Kempere, and Michael Oliveri. Students are encouraged to submit an abstract for the Ruska Award. There is also the opportunity to participate in a pre-meeting workshop on plunge freezing and cryo-TEM at Emory University, a Vendors' Social on May 18, and a banquet on May 19. Visit us at www.southeasternmicroscopy.org for details. Please come and join us!
All microscopists in the southeast region, irrespective of discipline, are invited and encouraged to join the Southeast Microscopy Society. SEMS is one of the most active Local Affiliate Societies of the Microscopy Society of America and an excellent venue for students, technologists and faculty to share their data with and to meet other scientists from our region with similar interests.
I am thinking about the EM radiation for an expecting monther. Is there any policies regarding the use of SEM and TEM by pregant employees? Thank you very much!
X
==============================Original Headers============================== 4, 21 -- From Xiaolan.Wu-at-Dartmouth.edu Tue Feb 15 18:44:59 2011 4, 21 -- Received: from mailhub2.dartmouth.edu (mailhub2.dartmouth.edu [129.170.17.107]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1G0ixhM019777 4, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 18:44:59 -0600 4, 21 -- Received: from newdonner.Dartmouth.EDU (newdonner.Dartmouth.EDU [129.170.208.35]) 4, 21 -- by mailhub2.dartmouth.edu (8.13.5/DND2.0/8.13.5) with ESMTP id p1FMNRSC020983 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 19:44:53 -0500 4, 21 -- Message-id: {259617186-at-newdonner.Dartmouth.EDU} 4, 21 -- Date: 15 Feb 2011 19:44:53 -0500 4, 21 -- From: Xiaolan.Wu-at-Dartmouth.edu (Xiaolan Wu) 4, 21 -- Subject: EM radiation 4, 21 -- To: Microscopy-at-microscopy.com 4, 21 -- X-Mailer: BlitzMail=?ISO-8859-1?Q?=AE?= version 2.6.6a1/blitzserv 3.34 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; charset=iso-8859-1 4, 21 -- Content-Disposition: inline 4, 21 -- X-MailScanner: Found to be clean by mailhub2.dartmouth.edu 4, 21 -- X-MailScanner-From: xiaolan.wu-at-dartmouth.edu 4, 21 -- Content-Transfer-Encoding: 8bit 4, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1G0ixhM019777 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jegoolsby-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jegoolsby-at-hotmail.com Name: james goolsby
Organization: Paragon Bioservices
Title-Subject: [Filtered] LKB ultratome service & manual
Message: I am looking for someone who can service a LKB NOVA ultratome.
It's been sitting in a warehouse 10+years and needs some cleaning and lubricating. Also, I would like to get the manual.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kajla-at-wisc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kajla-at-wisc.edu Name: mayur
Organization: UW Madison
Title-Subject: [Filtered] Lysozyme Message: Hi, i am have been trying to do immuno-EM on mosquito midguts, fixed in 4% PFA/0.2% Glut and embedded in LR White. I know that the lysozyme protein is supposed to be present in the midgut tissues (based on Western blotting) but i have never been able to detect it on the sectioned guts. Does anybody have any experience working with lysozyme detection ? Lysozyme is positively charged and supposed to be associated with the midgut laminae. thanks, mayur Login Host: 68.190.117.108 ---------------------------------------------------------------------------
==============================Original Headers============================== 6, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 16 05:28:26 2011 6, 25 -- Received: from znl.com ([206.69.208.20]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GBSQcl021259 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 05:28:26 -0600 6, 25 -- Received: from localhost (localhost [127.0.0.1]) 6, 25 -- by znl.com (Postfix) with ESMTP id 3ACC933557C 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 05:28:26 -0600 (CST) 6, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 6, 25 -- Received: from znl.com ([127.0.0.1]) 6, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 6, 25 -- with ESMTP id PNM7f+aTd+LU for {microscopy-at-microscopy.com} ; 6, 25 -- Wed, 16 Feb 2011 05:28:25 -0600 (CST) 6, 25 -- Received: from NestorPwrBk.local (b4ef2.static.pacific.net.au [125.255.46.242]) 6, 25 -- by znl.com (Postfix) with ESMTPA id 25DBA335571 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 05:28:24 -0600 (CST) 6, 25 -- Message-ID: {4D5BB4D7.2060306-at-microscopy.com} 6, 25 -- Date: Wed, 16 Feb 2011 22:28:23 +1100 6, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 6, 25 -- Reply-To: kajla-at-wisc.edu 6, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 25 -- MIME-Version: 1.0 6, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 6, 25 -- Subject: viaWWW:Lysozyme proteins 6, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello everyone Would anyone have a manual/operating instructions for the OM U2? We have inherited a dusty old relic & I'd like to try & get it working again rather than it sitting wasted in a corner. Many Thanks Nikki WestonThis message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.
This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 1, 34 -- From Nicola.Weston-at-nottingham.ac.uk Wed Feb 16 06:09:31 2011 1, 34 -- Received: from thb-mta-19.emailfiltering.com (thb-mta-19-tx.emailfiltering.com [194.116.199.150]) 1, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GC9Ua2021270 1, 34 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 06:09:31 -0600 1, 34 -- Received: from smtp1.nottingham.ac.uk ([128.243.44.4]) 1, 34 -- by thb-mta-19.emailfiltering.com with emfmta (version 4.8.0.417) vanilla id 1831944434 1, 34 -- for microscopy-at-microscopy.com;2f6ae740ac3ac8f4; Wed, 16 Feb 2011 12:09:30 +0000 1, 34 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 1, 34 -- by smtp1.nottingham.ac.uk with esmtp (Exim 4.60) 1, 34 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 1, 34 -- id 1PpgCH-0002tz-W2 1, 34 -- for microscopy-at-microscopy.com; Wed, 16 Feb 2011 12:09:30 +0000 1, 34 -- Received: from UIWEXHUB01.ad.nottingham.ac.uk ([128.243.15.133]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 1, 34 -- Wed, 16 Feb 2011 12:09:08 +0000 1, 34 -- Received: from EXCHANGE1.ad.nottingham.ac.uk ([fe80::7962:f868:e6ee:6267]) by 1, 34 -- UIWEXHUB01.ad.nottingham.ac.uk ([2002:80f3:f85::80f3:f85]) with mapi; Wed, 16 1, 34 -- Feb 2011 12:09:08 +0000 1, 34 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 1, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 1, 34 -- Date: Wed, 16 Feb 2011 12:09:07 +0000 1, 34 -- Subject: Reichart OM U2 ultramicrotome 1, 34 -- Thread-Topic: Reichart OM U2 ultramicrotome 1, 34 -- Thread-Index: AcvN0k/gREHGWJN4QEqmZCvhg24M+w== 1, 34 -- Message-ID: {443CA551C991D84AB0FF11A607D4D46C0293458118-at-EXCHANGE1.ad.nottingham.ac.uk} 1, 34 -- Accept-Language: en-US, en-GB 1, 34 -- Content-Language: en-US 1, 34 -- X-MS-Has-Attach: 1, 34 -- X-MS-TNEF-Correlator: 1, 34 -- acceptlanguage: en-US, en-GB 1, 34 -- Content-Type: text/plain; charset="us-ascii" 1, 34 -- MIME-Version: 1.0 1, 34 -- X-OriginalArrivalTime: 16 Feb 2011 12:09:09.0078 (UTC) FILETIME=[50B0C760:01CBCDD2] 1, 34 -- Content-Transfer-Encoding: 8bit 1, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GC9Ua2021270 ==============================End of - Headers==============================
} I am thinking about the EM radiation for an expecting monther. } Is there any policies regarding the use of SEM and TEM by pregant } employees? Thank you very much!
in our Facility (located in Austria) we had the pleasure to deal with this situation a year ago.
What we found out was that with the maximum X-ray emission of our 100 and 300 kV TEMs as measured in the acceptance test (approx. 200 nSv/h), it takes an expecting mother under intensive, but realistic working conditions more than 2 years to reach the maximum dose allowed here by law.
To confirm this, we had both microscopes re-measured by an external, certified expert. It turned out that close to our 100 kV machine, the radiation level is even below environmental radiation, as the column shields more than it emits. Subsequently, we received an official safety clearance from the local work inspectorate for both scopes.
We also had our pregnant colleagues carry live dosimeters with them, which we checked daily, to make sure the conditions at the microscopes did not change.
I hope that information helps, all the best,
Guenter
-- Dr. Guenter Resch, IMP-IMBA-GMI Electron Microscopy Facility Email: guenter.resch-at-imba.oeaw.ac.at; Web: http://cores.imp.ac.at/em Phone: +43 (1) 79044-4250; Fax/Voice Mail: +43 (1) 79044-224250 Post: Dr. Bohr-Gasse 3, 1030 Vienna, Austria, European Union
==============================Original Headers============================== 9, 26 -- From guenter.resch-at-imba.oeaw.ac.at Wed Feb 16 06:30:46 2011 9, 26 -- Received: from mail.imp.ac.at (mail.imp.ac.at [193.171.188.9]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GCUjwF005429 9, 26 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 06:30:46 -0600 9, 26 -- Received: from impexchfront.imp.univie.ac.at (impexchfront.imp.univie.ac.at [10.42.40.110]) 9, 26 -- by mail.imp.ac.at (Postfix) with ESMTP id 39A44182201; 9, 26 -- Wed, 16 Feb 2011 13:30:42 +0100 (CET) 9, 26 -- Received: from emf01.imp.univie.ac.at (10.50.132.103) by 9, 26 -- webmail.imp.univie.ac.at (10.42.40.147) with Microsoft SMTP Server (TLS) id 9, 26 -- 8.3.137.0; Wed, 16 Feb 2011 13:30:41 +0100 9, 26 -- Date: Wed, 16 Feb 2011 13:30:41 +0100 9, 26 -- From: Guenter Resch {guenter.resch-at-imba.oeaw.ac.at} 9, 26 -- To: {Xiaolan.Wu-at-Dartmouth.edu} , {Microscopy-at-microscopy.com} 9, 26 -- Subject: Re: [Microscopy] EM radiation 9, 26 -- Message-ID: {20110216133041.40e46f2d-at-emf01.imp.univie.ac.at} 9, 26 -- In-Reply-To: {201102160053.p1G0rIhd000691-at-ns.microscopy.com} 9, 26 -- References: {201102160053.p1G0rIhd000691-at-ns.microscopy.com} 9, 26 -- X-Mailer: Claws Mail 3.5.0 (GTK+ 2.12.12; x86_64-pc-linux-gnu) 9, 26 -- MIME-Version: 1.0 9, 26 -- Content-Type: text/plain; charset="US-ASCII" 9, 26 -- Content-Transfer-Encoding: 7bit 9, 26 -- X-IMP-MailScanner-Information: Please contact the ISP for more information 9, 26 -- X-IMP-MailScanner-ID: 39A44182201.12AE3 9, 26 -- X-IMP-MailScanner: Found to be clean 9, 26 -- X-IMP-MailScanner-From: guenter.resch-at-imba.oeaw.ac.at 9, 26 -- X-Spam-Status: No ==============================End of - Headers==============================
An EM should be checked for leaks as part of its regular servicing, and any time the column's been taken apart so radiation from it should never be an issue. Far more of a concern I'd say are the chemicals we typically use in EM and which many users get complacent about handling. We use a lot of rather nasty solvents, heavy metals and other compounds that could be a real threat to an unborn child. Cheers Ian
Ian Portman Imaging Suite Manager School of Life Sciences University of Warwick
-----Original Message----- X-from: Xiaolan.Wu-at-dartmouth.edu [mailto:Xiaolan.Wu-at-dartmouth.edu] Sent: 16 February 2011 00:52 To: Portman, Ian
Hi All,
I am thinking about the EM radiation for an expecting monther. Is there any policies regarding the use of SEM and TEM by pregant employees? Thank you very much!
X
==============================Original Headers============================== 16, 39 -- From I.J.Portman-at-warwick.ac.uk Wed Feb 16 06:45:51 2011 16, 39 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8]) 16, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GCjpUQ021776 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 06:45:51 -0600 16, 39 -- Received: from localhost (localhost [127.0.0.1]) 16, 39 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p1GCjocJ018547 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 12:45:50 GMT 16, 39 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 16, 39 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1]) 16, 39 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 16, 39 -- with LMTP id 0U4kDt0yleYq for {Microscopy-at-microscopy.com} ; 16, 39 -- Wed, 16 Feb 2011 12:45:50 +0000 (GMT) 16, 39 -- Received: from ash.ads.warwick.ac.uk (ash [137.205.97.162]) 16, 39 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p1GCjnZE018511 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 12:45:49 GMT 16, 39 -- X-Envelope-From: I.J.Portman-at-warwick.ac.uk 16, 39 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.181]) by ash.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 16, 39 -- Wed, 16 Feb 2011 12:45:48 +0000 16, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 39 -- Content-class: urn:content-classes:message 16, 39 -- MIME-Version: 1.0 16, 39 -- Content-Type: text/plain; 16, 39 -- charset="us-ascii" 16, 39 -- Subject: RE: [Microscopy] EM radiation 16, 39 -- Date: Wed, 16 Feb 2011 12:45:48 -0000 16, 39 -- Message-ID: {DB126BA68A8A5A439BAF072438A39FEA0294DAE0-at-ELDER.ads.warwick.ac.uk} 16, 39 -- In-Reply-To: {201102160051.p1G0pq53030529-at-ns.microscopy.com} 16, 39 -- X-MS-Has-Attach: 16, 39 -- X-MS-TNEF-Correlator: 16, 39 -- Thread-Topic: [Microscopy] EM radiation 16, 39 -- Thread-Index: AcvNc7TzkfryGWHzQ0uym1zBXbTM8QAYukZA 16, 39 -- References: {201102160051.p1G0pq53030529-at-ns.microscopy.com} 16, 39 -- From: "Portman, Ian" {I.J.Portman-at-warwick.ac.uk} 16, 39 -- To: {Microscopy-at-microscopy.com} 16, 39 -- Cc: "Harvey-Smith, Phillip" {P.Harvey-Smith-at-warwick.ac.uk} 16, 39 -- X-OriginalArrivalTime: 16 Feb 2011 12:45:48.0756 (UTC) FILETIME=[6FCD0140:01CBCDD7] 16, 39 -- X-DCC-Warwick-Metrics: nightshade; whitelist 16, 39 -- Content-Transfer-Encoding: 8bit 16, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GCjpUQ021776 ==============================End of - Headers==============================
Hello Everyone, A number of years ago we faced the same question at Goodyear and the same issues were raised and the same answers were found. Management elected to provide anyone who wanted to use it a lead x-ray apron. We had one scope so on could be shared quite easily. The weight discourages all but the most concerned.
I suspect for peace of mind and to demonstrate the goodwill of the organization to go beyond any safety regulation to promote safety, having a lead apron available and a short video training course on radiation would solve all your concerns.
stay safe... Frank
I.J.Portman-at-warwi ck.ac.uk To 02/16/2011 07:51 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] RE: EM radiation I.J.Portman-at-warwi ck.ac.uk
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
An EM should be checked for leaks as part of its regular servicing, and any time the column's been taken apart so radiation from it should never be an issue. Far more of a concern I'd say are the chemicals we typically use in EM and which many users get complacent about handling. We use a lot of rather nasty solvents, heavy metals and other compounds that could be a real threat to an unborn child. Cheers Ian
Ian Portman Imaging Suite Manager School of Life Sciences University of Warwick
-----Original Message----- X-from: Xiaolan.Wu-at-dartmouth.edu [mailto:Xiaolan.Wu-at-dartmouth.edu] Sent: 16 February 2011 00:52 To: Portman, Ian
Hi All,
I am thinking about the EM radiation for an expecting monther. Is there any policies regarding the use of SEM and TEM by pregant employees? Thank you very much!
X
==============================Original Headers============================== 16, 39 -- From I.J.Portman-at-warwick.ac.uk Wed Feb 16 06:45:51 2011 16, 39 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8]) 16, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GCjpUQ021776 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 06:45:51 -0600 16, 39 -- Received: from localhost (localhost [127.0.0.1]) 16, 39 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p1GCjocJ018547 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 12:45:50 GMT 16, 39 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 16, 39 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1]) 16, 39 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 16, 39 -- with LMTP id 0U4kDt0yleYq for {Microscopy-at-microscopy.com} ; 16, 39 -- Wed, 16 Feb 2011 12:45:50 +0000 (GMT) 16, 39 -- Received: from ash.ads.warwick.ac.uk (ash [137.205.97.162]) 16, 39 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p1GCjnZE018511 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 12:45:49 GMT 16, 39 -- X-Envelope-From: I.J.Portman-at-warwick.ac.uk 16, 39 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.181]) by ash.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 16, 39 -- Wed, 16 Feb 2011 12:45:48 +0000 16, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 39 -- Content-class: urn:content-classes:message 16, 39 -- MIME-Version: 1.0 16, 39 -- Content-Type: text/plain; 16, 39 -- charset="us-ascii" 16, 39 -- Subject: RE: [Microscopy] EM radiation 16, 39 -- Date: Wed, 16 Feb 2011 12:45:48 -0000 16, 39 -- Message-ID: {DB126BA68A8A5A439BAF072438A39FEA0294DAE0-at-ELDER.ads.warwick.ac.uk} 16, 39 -- In-Reply-To: {201102160051.p1G0pq53030529-at-ns.microscopy.com} 16, 39 -- X-MS-Has-Attach: 16, 39 -- X-MS-TNEF-Correlator: 16, 39 -- Thread-Topic: [Microscopy] EM radiation 16, 39 -- Thread-Index: AcvNc7TzkfryGWHzQ0uym1zBXbTM8QAYukZA 16, 39 -- References: {201102160051.p1G0pq53030529-at-ns.microscopy.com} 16, 39 -- From: "Portman, Ian" {I.J.Portman-at-warwick.ac.uk} 16, 39 -- To: {Microscopy-at-microscopy.com} 16, 39 -- Cc: "Harvey-Smith, Phillip" {P.Harvey-Smith-at-warwick.ac.uk} 16, 39 -- X-OriginalArrivalTime: 16 Feb 2011 12:45:48.0756 (UTC) FILETIME= [6FCD0140:01CBCDD7] 16, 39 -- X-DCC-Warwick-Metrics: nightshade; whitelist 16, 39 -- Content-Transfer-Encoding: 8bit 16, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GCjpUQ021776 ==============================End of - Headers==============================
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 35, 16 -- From frank_karl-at-lincolnelectric.com Wed Feb 16 07:04:02 2011 35, 16 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 35, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GD423I005942 35, 16 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:04:02 -0600 35, 16 -- In-Reply-To: {201102161251.p1GCp1B4032643-at-ns.microscopy.com} 35, 16 -- Subject: Re: [Microscopy] RE: EM radiation 35, 16 -- To: Microscopy-at-microscopy.com 35, 16 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 35, 16 -- Message-ID: {OF3971152C.55FEF3FF-ON85257839.0046BB30-85257839.0047C57C-at-lincolnelectric.com} 35, 16 -- From: Frank_Karl-at-lincolnelectric.com 35, 16 -- Date: Wed, 16 Feb 2011 08:03:58 -0500 35, 16 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 35, 16 -- 02/16/2011 08:03:57 AM 35, 16 -- MIME-Version: 1.0 35, 16 -- Content-Type: text/plain; 35, 16 -- charset="US-ASCII" ==============================End of - Headers==============================
What tricks/tools do people suggest for unscrewing objectives from the turret when you cannot loosen them by hand? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 24 -- From david.knecht-at-uconn.edu Wed Feb 16 07:51:02 2011 5, 24 -- Received: from mta31.charter.net (mta31.charter.net [216.33.127.82]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GDp1m4024520 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:51:01 -0600 5, 24 -- Received: from imp10 ([10.20.200.15]) by mta31.charter.net 5, 24 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP 5, 24 -- id {20110216135100.PQHH12685.mta31.charter.net-at-imp10} 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- Received: from [10.0.1.4] ([71.80.125.187]) 5, 24 -- by imp10 with smtp.charter.net 5, 24 -- id 8dqy1g00X42ikRj05dr0nv; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=kj9zAlcOel0A:10 a=O1RQMTG4XG3sO61s4HkA:9 5, 24 -- a=JK2UMX8g5W29FKWeE3o3bDKwXQ4A:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 5, 24 -- a=Rb0d9Ftq5AQA:10 5, 24 -- From: David Knecht charter {david.knecht-at-uconn.edu} 5, 24 -- Content-Type: text/plain; charset=us-ascii 5, 24 -- Subject: Removing stuck objectives 5, 24 -- Date: Wed, 16 Feb 2011 08:50:58 -0500 5, 24 -- Message-Id: {161CAA81-7F7F-4752-B540-FE389BB48246-at-uconn.edu} 5, 24 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 24 -- X-Mailer: Apple Mail (2.1082) 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GDp1m4024520 ==============================End of - Headers==============================
David, Buy a strap wrench. If the rubber slips on the objective barrel, use channel pliers to grab the rubber strap and turn. Don't squeeze excessively. Focus on turning with the pliers.
You may be able to use leather gloves with the pliers, but the teeth of the wrench could penetrate and cause gouges in the outer case. A small sheet of thick rubber may also work, but strap wrenches are cheap and store easily.
Good luck, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: Wednesday, February 16, 2011 8:59 AM To: Sobocinski, Gregg
What tricks/tools do people suggest for unscrewing objectives from the turret when you cannot loosen them by hand? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 24 -- From david.knecht-at-uconn.edu Wed Feb 16 07:51:02 2011 5, 24 -- Received: from mta31.charter.net (mta31.charter.net [216.33.127.82]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GDp1m4024520 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:51:01 -0600 5, 24 -- Received: from imp10 ([10.20.200.15]) by mta31.charter.net 5, 24 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP 5, 24 -- id {20110216135100.PQHH12685.mta31.charter.net-at-imp10} 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- Received: from [10.0.1.4] ([71.80.125.187]) 5, 24 -- by imp10 with smtp.charter.net 5, 24 -- id 8dqy1g00X42ikRj05dr0nv; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=kj9zAlcOel0A:10 a=O1RQMTG4XG3sO61s4HkA:9 5, 24 -- a=JK2UMX8g5W29FKWeE3o3bDKwXQ4A:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 5, 24 -- a=Rb0d9Ftq5AQA:10 5, 24 -- From: David Knecht charter {david.knecht-at-uconn.edu} 5, 24 -- Content-Type: text/plain; charset=us-ascii 5, 24 -- Subject: Removing stuck objectives 5, 24 -- Date: Wed, 16 Feb 2011 08:50:58 -0500 5, 24 -- Message-Id: {161CAA81-7F7F-4752-B540-FE389BB48246-at-uconn.edu} 5, 24 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 24 -- X-Mailer: Apple Mail (2.1082) 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GDp1m4024520 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 26 -- From greggps-at-umich.edu Wed Feb 16 08:23:34 2011 15, 26 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201]) 15, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GENYFh008991 15, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:23:34 -0600 15, 26 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.233]) by 15, 26 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Wed, 16 Feb 15, 26 -- 2011 09:23:32 -0500 15, 26 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} 15, 26 -- To: "david.knecht-at-uconn.edu" {david.knecht-at-uconn.edu} 15, 26 -- CC: Microscopy MSA {Microscopy-at-microscopy.com} 15, 26 -- Date: Wed, 16 Feb 2011 09:23:31 -0500 15, 26 -- Subject: RE: [Microscopy] Removing stuck objectives 15, 26 -- Thread-Topic: [Microscopy] Removing stuck objectives 15, 26 -- Thread-Index: AcvN4bmCN3RIlJ2ATPCOUkBl7WxG3QAAnpuw 15, 26 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA393425810EE14C-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} 15, 26 -- References: {201102161359.p1GDxQdD002922-at-ns.microscopy.com} 15, 26 -- In-Reply-To: {201102161359.p1GDxQdD002922-at-ns.microscopy.com} 15, 26 -- Accept-Language: en-US 15, 26 -- Content-Language: en-US 15, 26 -- X-MS-Has-Attach: 15, 26 -- X-MS-TNEF-Correlator: 15, 26 -- acceptlanguage: en-US 15, 26 -- Content-Type: text/plain; charset="us-ascii" 15, 26 -- MIME-Version: 1.0 15, 26 -- Content-Transfer-Encoding: 8bit 15, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GENYFh008991 ==============================End of - Headers==============================
I was recently approached by two different researchers about the possibility of observing and perhaps even analyzing bitumen in our electron microprobe. I initially recoiled at the thought of putting a tar-like mixture of petroleum in the microprobe, and I informed these researchers that they probably want a technique used in organic chemistry, like gas chromatography, to analyze such a viscous mixture of organic liquids. Still the intellectual challenge remains: can bitumen be stabilized enough to observe (low beam currents) or perhaps even analyze (high beam currents) in a microprobe? I know that bitumen can be "refined" (that is, the crude oil removed), leaving behind the heaviest fraction with a higher boiling point (about 525 C). Does anyone have experience with somehow stabilizing bitumen for observation/analysis in a non-environmental SEM/EPMA? I'm curious to hear about others' experiences, either successful or not, with this.
Best, Ellery
Ellery Frahm, Ph.D. Manager & Principal Analyst, Electron Microprobe Laboratory Research Associate, Department of Geology & Geophysics University of Minnesota - Twin Cities http://probelab.geo.umn.edu
==============================Original Headers============================== 9, 19 -- From frah0010-at-umn.edu Wed Feb 16 08:54:10 2011 9, 19 -- Received: from vs-m.tc.umn.edu (vs-m.tc.umn.edu [134.84.135.97]) 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GEsAHj025973 9, 19 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:54:10 -0600 9, 19 -- Received: from x-134-84-75-179.uofm-secure.wireless.umn.edu (x-134-84-75-179.uofm-secure.wireless.umn.edu [134.84.75.179]) 9, 19 -- by vs-m.tc.umn.edu (UMN smtpd) with ESMTP 9, 19 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:54:10 -0600 (CST) 9, 19 -- X-Umn-Remote-Mta: [N] x-134-84-75-179.uofm-secure.wireless.umn.edu [134.84.75.179] #+LO+TS+AU 9, 19 -- X-Umn-Classification: local 9, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} 9, 19 -- Content-Type: text/plain; charset=us-ascii 9, 19 -- Subject: bitumen in the SEM/EPMA? 9, 19 -- Date: Wed, 16 Feb 2011 08:54:09 -0600 9, 19 -- Message-Id: {DFCD89F7-7B62-486D-9003-CCC0C7FB80C6-at-umn.edu} 9, 19 -- To: microscopy-at-microscopy.com 9, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) 9, 19 -- X-Mailer: Apple Mail (2.1082) 9, 19 -- Content-Transfer-Encoding: 8bit 9, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GEsAHj025973 ==============================End of - Headers==============================
I had similar problem before. A Zeiss scope guy shared me a tip- wear lab gloves to get a better grip on the objective while loosening it from the turret.
Claire
Claire Haueter Electron Microscopy Technologist Jan and Dan Duncan Neurological Research Institute Howard Hughes Medical Institute 1250 Moursund Street, Suite 1125 Houston, TX 77030 Lab Phone: (832) 824-8772 EMAIL: chaueter-at-bcm.edu
________________________________________ X-from: david.knecht-at-uconn.edu [david.knecht-at-uconn.edu] Sent: Wednesday, February 16, 2011 8:01 AM To: Haueter, Claire Menoza
What tricks/tools do people suggest for unscrewing objectives from the turret when you cannot loosen them by hand? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 24 -- From david.knecht-at-uconn.edu Wed Feb 16 07:51:02 2011 5, 24 -- Received: from mta31.charter.net (mta31.charter.net [216.33.127.82]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GDp1m4024520 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:51:01 -0600 5, 24 -- Received: from imp10 ([10.20.200.15]) by mta31.charter.net 5, 24 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP 5, 24 -- id {20110216135100.PQHH12685.mta31.charter.net-at-imp10} 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- Received: from [10.0.1.4] ([71.80.125.187]) 5, 24 -- by imp10 with smtp.charter.net 5, 24 -- id 8dqy1g00X42ikRj05dr0nv; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=kj9zAlcOel0A:10 a=O1RQMTG4XG3sO61s4HkA:9 5, 24 -- a=JK2UMX8g5W29FKWeE3o3bDKwXQ4A:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 5, 24 -- a=Rb0d9Ftq5AQA:10 5, 24 -- From: David Knecht charter {david.knecht-at-uconn.edu} 5, 24 -- Content-Type: text/plain; charset=us-ascii 5, 24 -- Subject: Removing stuck objectives 5, 24 -- Date: Wed, 16 Feb 2011 08:50:58 -0500 5, 24 -- Message-Id: {161CAA81-7F7F-4752-B540-FE389BB48246-at-uconn.edu} 5, 24 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 24 -- X-Mailer: Apple Mail (2.1082) 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GDp1m4024520 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 33 -- From chaueter-at-bcm.edu Wed Feb 16 09:46:42 2011 11, 33 -- Received: from iron3.corp.bcm.tmc.edu (iron3.corp.bcm.tmc.edu [128.249.224.15]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GFkg72011445 11, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 09:46:42 -0600 11, 33 -- X-IronPort-AV: E=Sophos;i="4.60,480,1291615200"; 11, 33 -- d="scan'208";a="81243051" 11, 33 -- Received: from unknown (HELO EPW-EXHUB4-P023.ad.bcm.edu) ([10.22.132.90]) 11, 33 -- by iron3-out.bcm.edu with ESMTP; 16 Feb 2011 09:46:41 -0600 11, 33 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 11, 33 -- EPW-EXHUB4-P023.ad.bcm.edu ([::1]) with mapi; Wed, 16 Feb 2011 09:46:41 -0600 11, 33 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 11, 33 -- To: "david.knecht-at-uconn.edu" {david.knecht-at-uconn.edu} , 11, 33 -- "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 33 -- Date: Wed, 16 Feb 2011 09:46:40 -0600 11, 33 -- Subject: RE: [Microscopy] Removing stuck objectives 11, 33 -- Thread-Topic: [Microscopy] Removing stuck objectives 11, 33 -- Thread-Index: AcvN4hQ5iILgBVt3Qmuq3zbW0F4Q+AADcc1q 11, 33 -- Message-ID: {F3EEF3767DFF3244826E72285C1C273448FB731155-at-EXCMSMBX03.ad.bcm.edu} 11, 33 -- References: {201102161401.p1GE1wkC007107-at-ns.microscopy.com} 11, 33 -- In-Reply-To: {201102161401.p1GE1wkC007107-at-ns.microscopy.com} 11, 33 -- Accept-Language: en-US 11, 33 -- Content-Language: en-US 11, 33 -- X-MS-Has-Attach: 11, 33 -- X-MS-TNEF-Correlator: 11, 33 -- acceptlanguage: en-US 11, 33 -- x-tm-as-product-ver: SMEX-8.0.0.1307-6.500.1024-17960.000 11, 33 -- x-tm-as-result: No--44.299700-8.000000-31 11, 33 -- x-tm-as-user-approved-sender: No 11, 33 -- x-tm-as-user-blocked-sender: No 11, 33 -- Content-Type: text/plain; charset="us-ascii" 11, 33 -- MIME-Version: 1.0 11, 33 -- Content-Transfer-Encoding: 8bit 11, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GFkg72011445 ==============================End of - Headers==============================
Oil shales and other samples containing bitumin can be safely imaged using cryoSEM. In that case the sample is frozen and you see no change in the bitumin structure even under very intense beam. Thus amount of oil released is presumably quite low if at all.
Oil can also be imaged safely without cryo. I drilled a very small hole in a sample mount and filled it with oil. I then covered all with a formvar film to seal in the oil. This held up very well under low Vacuum for EDX analysis of the oil.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "frah0010-at-umn.edu" {frah0010-at-umn.edu} } Reply-To: "frah0010-at-umn.edu" {frah0010-at-umn.edu} } Date: Wed, 16 Feb 2011 09:55:51 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] bitumen in the SEM/EPMA? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Microscopy colleagues, } } I was recently approached by two different researchers about the possibility } of observing and perhaps even analyzing bitumen in our electron microprobe. I } initially recoiled at the thought of putting a tar-like mixture of petroleum } in the microprobe, and I informed these researchers that they probably want a } technique used in organic chemistry, like gas chromatography, to analyze such } a viscous mixture of organic liquids. Still the intellectual challenge } remains: can bitumen be stabilized enough to observe (low beam currents) or } perhaps even analyze (high beam currents) in a microprobe? I know that } bitumen can be "refined" (that is, the crude oil removed), leaving behind the } heaviest fraction with a higher boiling point (about 525 C). Does anyone have } experience with somehow stabilizing bitumen for observation/analysis in a } non-environmental SEM/EPMA? I'm curious to hear about others' experiences, } either successful or not, with this. } } Best, } Ellery } } } Ellery Frahm, Ph.D. } Manager & Principal Analyst, Electron Microprobe Laboratory } Research Associate, Department of Geology & Geophysics } University of Minnesota - Twin Cities } http://probelab.geo.umn.edu } } } } } } ==============================Original Headers============================== } 9, 19 -- From frah0010-at-umn.edu Wed Feb 16 08:54:10 2011 } 9, 19 -- Received: from vs-m.tc.umn.edu (vs-m.tc.umn.edu [134.84.135.97]) } 9, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1GEsAHj025973 } 9, 19 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:54:10 -0600 } 9, 19 -- Received: from x-134-84-75-179.uofm-secure.wireless.umn.edu } (x-134-84-75-179.uofm-secure.wireless.umn.edu [134.84.75.179]) } 9, 19 -- by vs-m.tc.umn.edu (UMN smtpd) with ESMTP } 9, 19 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:54:10 -0600 } (CST) } 9, 19 -- X-Umn-Remote-Mta: [N] x-134-84-75-179.uofm-secure.wireless.umn.edu } [134.84.75.179] #+LO+TS+AU } 9, 19 -- X-Umn-Classification: local } 9, 19 -- From: Ellery Frahm {frah0010-at-umn.edu} } 9, 19 -- Content-Type: text/plain; charset=us-ascii } 9, 19 -- Subject: bitumen in the SEM/EPMA? } 9, 19 -- Date: Wed, 16 Feb 2011 08:54:09 -0600 } 9, 19 -- Message-Id: {DFCD89F7-7B62-486D-9003-CCC0C7FB80C6-at-umn.edu} } 9, 19 -- To: microscopy-at-microscopy.com } 9, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) } 9, 19 -- X-Mailer: Apple Mail (2.1082) } 9, 19 -- Content-Transfer-Encoding: 8bit } 9, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p1GEsAHj025973 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 31 -- From dsherman-at-purdue.edu Wed Feb 16 10:03:09 2011 8, 31 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GG398E027862 8, 31 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 10:03:09 -0600 8, 31 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 8, 31 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p1GG38VH009821 8, 31 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 11:03:08 -0500 8, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.189]) by WPPEXHUB01F.purdue.lcl 8, 31 -- ([::1]) with mapi; Wed, 16 Feb 2011 11:03:08 -0500 8, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 8, 31 -- To: "frah0010-at-umn.edu" {frah0010-at-umn.edu} , 8, 31 -- "message to: MSA list" 8, 31 -- {microscopy-at-microscopy.com} 8, 31 -- Date: Wed, 16 Feb 2011 11:03:06 -0500 8, 31 -- Subject: Re: [Microscopy] bitumen in the SEM/EPMA? 8, 31 -- Thread-Topic: [Microscopy] bitumen in the SEM/EPMA? 8, 31 -- Thread-Index: AcvN6Ztwx8SbpGQZTLu4qTLiyAg6TQACWPqv 8, 31 -- Message-ID: {C9815F6A.BBA9%dsherman-at-purdue.edu} 8, 31 -- In-Reply-To: {201102161455.p1GEtpVx028851-at-ns.microscopy.com} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 8, 31 -- acceptlanguage: en-US 8, 31 -- Content-Type: text/plain; charset="us-ascii" 8, 31 -- MIME-Version: 1.0 8, 31 -- X-PMX-Version: 5.5.9.388399 8, 31 -- X-PerlMx-Virus-Scanned: Yes 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GG398E027862 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nickp-at-personifysearch.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nickp-at-personifysearch.com Name: Nick Pokoluk
Message: Our client, a major manufacturer of premiere microscopy products is looking to add several sales specialists, applications specialists, and marketing managers to their North American Commercial team. This is a group lead by scientists with a tremendous business acumen in this arena. We are currently looking for sales specialists in Florida, Georgia, North Carolina, South Carolina, Virginia, Pennsylvania, Texas, Alabama, Louisiana, and California. Ideal candidates will have some prior sales experience and outstanding microscopy hands on experience (will consider technical experts with no prior sales looking to move into the business side of the industry).
We are also seeking Applications Specialists for New England (MA, VT, NY), Southeast (Georgia, Tennessee, Alabama) and the Pacific Northwest (WA, OR). Ideal candidate will have technical expertise in microscopy techniques and will have trained users on equipment.
Interviews are set to begin immediately so please contact me soon. This is a retained search project and confidentiality will be adhered to at all times.
Thanks and good luck.
Nick Pokoluk Practice Director Personify nickp-at-personifysearch.com 800-875-6188 ext 105
I couldn't resist saying, "This is a galling problem for any seized pair of nut and bolt much less an objective and a turret. To solve the problem, first one must understand its natural roots. (http://en.wikipedia.org/wiki/Galling)
Galling as it may be, this is a problem in metallurgy and/or materials science, and, ultimately, real nano and 'elemental' science.
In either case, the easiest remedy would appear to be a life-long schedule of periodic 'flexing' of the 'bolt' in the grasp of the 'nut', and, thus, prevent the, now understood, galling consequences.
In the case of a neglected microscope objective, when galled, the objective seized within the threads of the turret illustrates a grievous form of instrumental negligence - typical, I'm embarrassed to say, of far too many of us who use and care for them.
Harsh remedies are called for, and with a strip of hard rubber (1/16" thick) wrapped around the objective and in the grasp of an appropriate pair of pliers (long handles for leverage), the turret immobilized by the left hand, and finally, the pliers (in the right!) drawn 'gently' (but inexorably) toward the operator, the objective generally succumbs to the force and will be freed. The rubber should prevent the teeth of the jaws of the pliers from 'reaching' the metal of the objective, but commodiously provide for sufficient 'grip' that the rubber can substitute as the wrench. I purchased mine in the plumbing section at Home Depot
Hopeful that the previous will be helpful to many, I have become, finally, 'hic finis est'.
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: chaueter-at-bcm.edu [mailto:chaueter-at-bcm.edu] Sent: Wednesday, February 16, 2011 10:53 AM To: Monson, Frederick
I had similar problem before. A Zeiss scope guy shared me a tip- wear lab gloves to get a better grip on the objective while loosening it from the turret.
Claire
Claire Haueter Electron Microscopy Technologist Jan and Dan Duncan Neurological Research Institute Howard Hughes Medical Institute 1250 Moursund Street, Suite 1125 Houston, TX 77030 Lab Phone: (832) 824-8772 EMAIL: chaueter-at-bcm.edu
________________________________________ X-from: david.knecht-at-uconn.edu [david.knecht-at-uconn.edu] Sent: Wednesday, February 16, 2011 8:01 AM To: Haueter, Claire Menoza
What tricks/tools do people suggest for unscrewing objectives from the turret when you cannot loosen them by hand? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 24 -- From david.knecht-at-uconn.edu Wed Feb 16 07:51:02 2011 5, 24 -- Received: from mta31.charter.net (mta31.charter.net [216.33.127.82]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GDp1m4024520 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:51:01 -0600 5, 24 -- Received: from imp10 ([10.20.200.15]) by mta31.charter.net 5, 24 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP 5, 24 -- id {20110216135100.PQHH12685.mta31.charter.net-at-imp10} 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- Received: from [10.0.1.4] ([71.80.125.187]) 5, 24 -- by imp10 with smtp.charter.net 5, 24 -- id 8dqy1g00X42ikRj05dr0nv; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=kj9zAlcOel0A:10 a=O1RQMTG4XG3sO61s4HkA:9 5, 24 -- a=JK2UMX8g5W29FKWeE3o3bDKwXQ4A:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 5, 24 -- a=Rb0d9Ftq5AQA:10 5, 24 -- From: David Knecht charter {david.knecht-at-uconn.edu} 5, 24 -- Content-Type: text/plain; charset=us-ascii 5, 24 -- Subject: Removing stuck objectives 5, 24 -- Date: Wed, 16 Feb 2011 08:50:58 -0500 5, 24 -- Message-Id: {161CAA81-7F7F-4752-B540-FE389BB48246-at-uconn.edu} 5, 24 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 24 -- X-Mailer: Apple Mail (2.1082) 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GDp1m4024520 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 33 -- From chaueter-at-bcm.edu Wed Feb 16 09:46:42 2011 11, 33 -- Received: from iron3.corp.bcm.tmc.edu (iron3.corp.bcm.tmc.edu [128.249.224.15]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GFkg72011445 11, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 09:46:42 -0600 11, 33 -- X-IronPort-AV: E=Sophos;i="4.60,480,1291615200"; 11, 33 -- d="scan'208";a="81243051" 11, 33 -- Received: from unknown (HELO EPW-EXHUB4-P023.ad.bcm.edu) ([10.22.132.90]) 11, 33 -- by iron3-out.bcm.edu with ESMTP; 16 Feb 2011 09:46:41 -0600 11, 33 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 11, 33 -- EPW-EXHUB4-P023.ad.bcm.edu ([::1]) with mapi; Wed, 16 Feb 2011 09:46:41 -0600 11, 33 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 11, 33 -- To: "david.knecht-at-uconn.edu" {david.knecht-at-uconn.edu} , 11, 33 -- "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 33 -- Date: Wed, 16 Feb 2011 09:46:40 -0600 11, 33 -- Subject: RE: [Microscopy] Removing stuck objectives 11, 33 -- Thread-Topic: [Microscopy] Removing stuck objectives 11, 33 -- Thread-Index: AcvN4hQ5iILgBVt3Qmuq3zbW0F4Q+AADcc1q 11, 33 -- Message-ID: {F3EEF3767DFF3244826E72285C1C273448FB731155-at-EXCMSMBX03.ad.bcm.edu} 11, 33 -- References: {201102161401.p1GE1wkC007107-at-ns.microscopy.com} 11, 33 -- In-Reply-To: {201102161401.p1GE1wkC007107-at-ns.microscopy.com} 11, 33 -- Accept-Language: en-US 11, 33 -- Content-Language: en-US 11, 33 -- X-MS-Has-Attach: 11, 33 -- X-MS-TNEF-Correlator: 11, 33 -- acceptlanguage: en-US 11, 33 -- x-tm-as-product-ver: SMEX-8.0.0.1307-6.500.1024-17960.000 11, 33 -- x-tm-as-result: No--44.299700-8.000000-31 11, 33 -- x-tm-as-user-approved-sender: No 11, 33 -- x-tm-as-user-blocked-sender: No 11, 33 -- Content-Type: text/plain; charset="us-ascii" 11, 33 -- MIME-Version: 1.0 11, 33 -- Content-Transfer-Encoding: 8bit 11, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GFkg72011445 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 36 -- From FMonson-at-wcupa.edu Wed Feb 16 15:48:14 2011 28, 36 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) 28, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GLmErw020971 28, 36 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 15:48:14 -0600 28, 36 -- X-ASG-Debug-ID: 1297892893-0368bc101d1bb010001-4CH8be 28, 36 -- Received: from WCU-XCH-05.PASSHE.LCL (wcu-xch-05.wcupa.edu [144.26.0.89]) by MX01.WCUPA.EDU with ESMTP id tLCTAdhFXCImKvB4 (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 16:48:13 -0500 (EST) 28, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 28, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.89 28, 36 -- X-ASG-Whitelist: Client 28, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 28, 36 -- WCU-XCH-05.PASSHE.LCL ([fe80::8068:be0d:980f:1540%16]) with mapi id 28, 36 -- 14.01.0270.001; Wed, 16 Feb 2011 16:48:13 -0500 28, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 28, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 28, 36 -- Subject: RE: [Microscopy] RE: Removing stuck objectives 28, 36 -- Thread-Topic: [Microscopy] RE: Removing stuck objectives 28, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: Removing stuck objectives 28, 36 -- Thread-Index: AQHLzfGjDNkrdKfUJk2+UR6HjeXI1JQEo2RA 28, 36 -- Date: Wed, 16 Feb 2011 21:48:11 +0000 28, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C02256A-at-WCU-XCH-03.PASSHE.LCL} 28, 36 -- References: {201102161553.p1GFrJrv024390-at-ns.microscopy.com} 28, 36 -- In-Reply-To: {201102161553.p1GFrJrv024390-at-ns.microscopy.com} 28, 36 -- Accept-Language: en-US 28, 36 -- Content-Language: en-US 28, 36 -- X-MS-Has-Attach: 28, 36 -- X-MS-TNEF-Correlator: 28, 36 -- x-originating-ip: [10.28.57.79] 28, 36 -- Content-Type: text/plain; charset="us-ascii" 28, 36 -- MIME-Version: 1.0 28, 36 -- X-Barracuda-Connect: wcu-xch-05.wcupa.edu[144.26.0.89] 28, 36 -- X-Barracuda-Start-Time: 1297892893 28, 36 -- X-Barracuda-Encrypted: AES128-SHA 28, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 28, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 28, 36 -- Content-Transfer-Encoding: 8bit 28, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GLmErw020971 ==============================End of - Headers==============================
In my experience, all safety issues are handled locally. That is, if you have a radon monitor handy, it should double as a convenient check for radiation above background. ( http://www.nukepills.com/radiation-detector.htm )$34.95 each for a card in the pocket? Individual must determine whether any device is sensitive enough.
I have tested 25 year old analytic x-ray equipment in addition to my 2002 FEI Quanta and Tecnai with not one example of radiation detection above background in areas well away from the instruments.
Please note that the chemicals (especially the heavy metals) used in biologic microscopy constitute the largest proportion of the hazard in a laboratory environment. Vacuum pump oils (are oils), specimens that are poorly characterized or sterilized. Yesterday's killed viral specimen spill and/or aerosol.
Thus, in the end, the issue of safety comes down to personal requirements of the employed, the impact on the normal tasks for which there are no substitutes, and institutional policy and, more important, hard evidence of NO hazard in the work place in question.
Pretty sure none of the above will improve the situation or completely answer the question.
How much more or less hazardous is a walk in the park than a day in the lab. I would not choose to be the one to say.
Regards,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: Xiaolan.Wu-at-Dartmouth.edu [mailto:Xiaolan.Wu-at-Dartmouth.edu] Sent: Tuesday, February 15, 2011 7:53 PM To: Monson, Frederick
Hi All,
I am thinking about the EM radiation for an expecting monther. Is there any policies regarding the use of SEM and TEM by pregant employees? Thank you very much!
X
==============================Original Headers============================== 4, 21 -- From Xiaolan.Wu-at-Dartmouth.edu Tue Feb 15 18:44:59 2011 4, 21 -- Received: from mailhub2.dartmouth.edu (mailhub2.dartmouth.edu [129.170.17.107]) 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1G0ixhM019777 4, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 18:44:59 -0600 4, 21 -- Received: from newdonner.Dartmouth.EDU (newdonner.Dartmouth.EDU [129.170.208.35]) 4, 21 -- by mailhub2.dartmouth.edu (8.13.5/DND2.0/8.13.5) with ESMTP id p1FMNRSC020983 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Feb 2011 19:44:53 -0500 4, 21 -- Message-id: {259617186-at-newdonner.Dartmouth.EDU} 4, 21 -- Date: 15 Feb 2011 19:44:53 -0500 4, 21 -- From: Xiaolan.Wu-at-Dartmouth.edu (Xiaolan Wu) 4, 21 -- Subject: EM radiation 4, 21 -- To: Microscopy-at-microscopy.com 4, 21 -- X-Mailer: BlitzMail=?ISO-8859-1?Q?=AE?= version 2.6.6a1/blitzserv 3.34 4, 21 -- MIME-Version: 1.0 4, 21 -- Content-Type: text/plain; charset=iso-8859-1 4, 21 -- Content-Disposition: inline 4, 21 -- X-MailScanner: Found to be clean by mailhub2.dartmouth.edu 4, 21 -- X-MailScanner-From: xiaolan.wu-at-dartmouth.edu 4, 21 -- Content-Transfer-Encoding: 8bit 4, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1G0ixhM019777 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 36 -- From FMonson-at-wcupa.edu Wed Feb 16 16:12:46 2011 21, 36 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 21, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GMCjYg004919 21, 36 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 16:12:45 -0600 21, 36 -- X-ASG-Debug-ID: 1297894337-0378c347aa1ac170001-4CH8be 21, 36 -- Received: from WCU-XCH-07.PASSHE.LCL (wcu-xch-07.wcupa.edu [144.26.0.91]) by MX02.WCUPA.EDU with ESMTP id bx7zC9oaUWAVPn2s (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 17:12:17 -0500 (EST) 21, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 21, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.91 21, 36 -- X-ASG-Whitelist: Client 21, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 21, 36 -- WCU-XCH-07.PASSHE.LCL ([fe80::3022:320a:c55f:f489%16]) with mapi id 21, 36 -- 14.01.0270.001; Wed, 16 Feb 2011 17:12:17 -0500 21, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 21, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 21, 36 -- Subject: RE: [Microscopy] EM radiation 21, 36 -- Thread-Topic: [Microscopy] EM radiation 21, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] EM radiation 21, 36 -- Thread-Index: AQHLzXPemYTHcvmDg0Wbad9Z7tMBVJQErCWA 21, 36 -- Date: Wed, 16 Feb 2011 22:12:17 +0000 21, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C0225D0-at-WCU-XCH-03.PASSHE.LCL} 21, 36 -- References: {201102160053.p1G0r03n032572-at-ns.microscopy.com} 21, 36 -- In-Reply-To: {201102160053.p1G0r03n032572-at-ns.microscopy.com} 21, 36 -- Accept-Language: en-US 21, 36 -- Content-Language: en-US 21, 36 -- X-MS-Has-Attach: 21, 36 -- X-MS-TNEF-Correlator: 21, 36 -- x-originating-ip: [10.28.57.79] 21, 36 -- Content-Type: text/plain; charset="us-ascii" 21, 36 -- MIME-Version: 1.0 21, 36 -- X-Barracuda-Connect: wcu-xch-07.wcupa.edu[144.26.0.91] 21, 36 -- X-Barracuda-Start-Time: 1297894337 21, 36 -- X-Barracuda-Encrypted: AES128-SHA 21, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 21, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 21, 36 -- Content-Transfer-Encoding: 8bit 21, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GMCjYg004919 ==============================End of - Headers==============================
well actually the short video course on radiation should definitely demonstrate the uselessness of a lead apron in this case! So to summary: as long as the radiation has been regularly tested, it is safe to work with TEM for a pregnant women. Actually much safer than to take intercontinental flights. I would just avoid to stay in front of the sample holder aperture while the beam is on (even without being pregnant)...
regards,
Stephane
----- Original Message ---- X-from: "Frank_Karl-at-lincolnelectric.com" {Frank_Karl-at-lincolnelectric.com} To: nizets2-at-yahoo.com Sent: Wed, February 16, 2011 2:06:56 PM
Hello Everyone, A number of years ago we faced the same question at Goodyear and the same issues were raised and the same answers were found. Management elected to provide anyone who wanted to use it a lead x-ray apron. We had one scope so on could be shared quite easily. The weight discourages all but the most concerned.
I suspect for peace of mind and to demonstrate the goodwill of the organization to go beyond any safety regulation to promote safety, having a lead apron available and a short video training course on radiation would solve all your concerns.
stay safe... Frank
I.J.Portman-at-warwi ck.ac.uk To 02/16/2011 07:51 frank_karl-at-lincolnelectric.com AM cc
Subject Please respond to [Microscopy] RE: EM radiation I.J.Portman-at-warwi ck.ac.uk
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
An EM should be checked for leaks as part of its regular servicing, and any time the column's been taken apart so radiation from it should never be an issue. Far more of a concern I'd say are the chemicals we typically use in EM and which many users get complacent about handling. We use a lot of rather nasty solvents, heavy metals and other compounds that could be a real threat to an unborn child. Cheers Ian
Ian Portman Imaging Suite Manager School of Life Sciences University of Warwick
-----Original Message----- X-from: Xiaolan.Wu-at-dartmouth.edu [mailto:Xiaolan.Wu-at-dartmouth.edu] Sent: 16 February 2011 00:52 To: Portman, Ian
Hi All,
I am thinking about the EM radiation for an expecting monther. Is there any policies regarding the use of SEM and TEM by pregant employees? Thank you very much!
X
==============================Original Headers============================== 16, 39 -- From I.J.Portman-at-warwick.ac.uk Wed Feb 16 06:45:51 2011 16, 39 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8]) 16, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GCjpUQ021776 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 06:45:51 -0600 16, 39 -- Received: from localhost (localhost [127.0.0.1]) 16, 39 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p1GCjocJ018547 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 12:45:50 GMT 16, 39 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 16, 39 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1]) 16, 39 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 16, 39 -- with LMTP id 0U4kDt0yleYq for {Microscopy-at-microscopy.com} ; 16, 39 -- Wed, 16 Feb 2011 12:45:50 +0000 (GMT) 16, 39 -- Received: from ash.ads.warwick.ac.uk (ash [137.205.97.162]) 16, 39 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p1GCjnZE018511 16, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 12:45:49 GMT 16, 39 -- X-Envelope-From: I.J.Portman-at-warwick.ac.uk 16, 39 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.181]) by ash.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 16, 39 -- Wed, 16 Feb 2011 12:45:48 +0000 16, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 16, 39 -- Content-class: urn:content-classes:message 16, 39 -- MIME-Version: 1.0 16, 39 -- Content-Type: text/plain; 16, 39 -- charset="us-ascii" 16, 39 -- Subject: RE: [Microscopy] EM radiation 16, 39 -- Date: Wed, 16 Feb 2011 12:45:48 -0000 16, 39 -- Message-ID: {DB126BA68A8A5A439BAF072438A39FEA0294DAE0-at-ELDER.ads.warwick.ac.uk} 16, 39 -- In-Reply-To: {201102160051.p1G0pq53030529-at-ns.microscopy.com} 16, 39 -- X-MS-Has-Attach: 16, 39 -- X-MS-TNEF-Correlator: 16, 39 -- Thread-Topic: [Microscopy] EM radiation 16, 39 -- Thread-Index: AcvNc7TzkfryGWHzQ0uym1zBXbTM8QAYukZA 16, 39 -- References: {201102160051.p1G0pq53030529-at-ns.microscopy.com} 16, 39 -- From: "Portman, Ian" {I.J.Portman-at-warwick.ac.uk} 16, 39 -- To: {Microscopy-at-microscopy.com} 16, 39 -- Cc: "Harvey-Smith, Phillip" {P.Harvey-Smith-at-warwick.ac.uk} 16, 39 -- X-OriginalArrivalTime: 16 Feb 2011 12:45:48.0756 (UTC) FILETIME= [6FCD0140:01CBCDD7] 16, 39 -- X-DCC-Warwick-Metrics: nightshade; whitelist 16, 39 -- Content-Transfer-Encoding: 8bit 16, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GCjpUQ021776 ==============================End of - Headers==============================
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 35, 16 -- From frank_karl-at-lincolnelectric.com Wed Feb 16 07:04:02 2011 35, 16 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 35, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GD423I005942 35, 16 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:04:02 -0600 35, 16 -- In-Reply-To: {201102161251.p1GCp1B4032643-at-ns.microscopy.com} 35, 16 -- Subject: Re: [Microscopy] RE: EM radiation 35, 16 -- To: Microscopy-at-microscopy.com 35, 16 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 35, 16 -- Message-ID: {OF3971152C.55FEF3FF-ON85257839.0046BB30-85257839.0047C57C-at-lincolnelectric.com} 35, 16 -- From: Frank_Karl-at-lincolnelectric.com 35, 16 -- Date: Wed, 16 Feb 2011 08:03:58 -0500 35, 16 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 35, 16 -- 02/16/2011 08:03:57 AM 35, 16 -- MIME-Version: 1.0 35, 16 -- Content-Type: text/plain; 35, 16 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 49, 37 -- From nizets2-at-yahoo.com Thu Feb 17 07:26:51 2011 49, 37 -- Received: from nm20.bullet.mail.sp2.yahoo.com (nm20.bullet.mail.sp2.yahoo.com [98.139.91.90]) 49, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1HDQowi009391 49, 37 -- for {microscopy-at-microscopy.com} ; Thu, 17 Feb 2011 07:26:50 -0600 49, 37 -- Received: from [98.139.91.66] by nm20.bullet.mail.sp2.yahoo.com with NNFMP; 17 Feb 2011 13:26:50 -0000 49, 37 -- Received: from [98.139.91.54] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 17 Feb 2011 13:26:50 -0000 49, 37 -- Received: from [127.0.0.1] by omp1054.mail.sp2.yahoo.com with NNFMP; 17 Feb 2011 13:26:50 -0000 49, 37 -- X-Yahoo-Newman-Property: ymail-3 49, 37 -- X-Yahoo-Newman-Id: 259264.9637.bm-at-omp1054.mail.sp2.yahoo.com 49, 37 -- Received: (qmail 95815 invoked by uid 60001); 17 Feb 2011 13:26:49 -0000 49, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1297949209; bh=yJLCPxuWxFnpH3Bxq8CE7lFyRccDP61sV/OR+LiM+rU=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=T/L6uIY6B1KOM0CItFnnjhtK9g5RmscerbVslEJh3dIxzUehTW6VvOTwPw/sxodLkUCcg9h1q+QADMFsDT+leBQJangrOx+mE0B2Zsu7wa5BaVPtFEX7fgJWNC4thyHSGGeE6aGCTES6LGzvkbctAuios8M37tmpwFYapzZPHQo= 49, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 49, 37 -- s=s1024; d=yahoo.com; 49, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 49, 37 -- b=FxPbkG4MpJIrjhT/0QVd2bhMTOIAGHptFpPvKabGaGJYDSREVI55x7C6/ZiyqLoT6vDaYcjdxFkZKw9iFw2mspwvt79DI+u/tcqI0BGZMQBW+TSsrhCIa0Tprsaz9AWi/JAVgl5zk7qUIRJN53hqdy988xhbBA7g9Bz8LVZtWjM=; 49, 37 -- Message-ID: {531857.95227.qm-at-web110810.mail.gq1.yahoo.com} 49, 37 -- X-YMail-OSG: a.KGYvsVM1nLr4d086nKuLoE1jOgLN0Z5b5n1wLVfqaRkOY 49, 37 -- S5xRWlHEvXxHdxgOAx1QGao5SQwG25RvFd98EMAiSGCbVoLX.oZBhZci2Lqz 49, 37 -- ncpglGYB2Ye5vZmHB.vRsfau4LPjPZMignjsxFNnlIip1MvzBBp.5LW2J0kl 49, 37 -- KtpdR1oOyePdXVFxSe8Ii.49Lj8hEWYBZ9r1kBkkL0.rKuZwkvJGHyfONkSD 49, 37 -- vMNUT_qePcjFDgjrCEjlMMu4vczGjnP8k__NgX6oUQ4Dga14rsPUXkvpJLtG 49, 37 -- Sd9ZCJQN82zOsNpzaQBpNVQfS7JKkN3RAQxOwcE_yNisumVLHbdmsH10Cs.m 49, 37 -- PwVsuwPgGCy.9PNQgNUEHk5PvEuZuc4s86MIV1P_B_7HNdggYrmXfGAWnfAO 49, 37 -- DXoH5qr.pd2Ah2ng- 49, 37 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Thu, 17 Feb 2011 05:26:49 PST 49, 37 -- X-Mailer: YahooMailRC/555 YahooMailWebService/0.8.109.292656 49, 37 -- References: {201102161306.p1GD6uHq011840-at-ns.microscopy.com} 49, 37 -- Date: Thu, 17 Feb 2011 05:26:49 -0800 (PST) 49, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 49, 37 -- Subject: Re: [Microscopy] EM radiation 49, 37 -- To: Frank_Karl-at-lincolnelectric.com 49, 37 -- Cc: microscopy-at-microscopy.com 49, 37 -- In-Reply-To: {201102161306.p1GD6uHq011840-at-ns.microscopy.com} 49, 37 -- MIME-Version: 1.0 49, 37 -- Content-Type: text/plain; charset=iso-8859-1 49, 37 -- Content-Transfer-Encoding: 8bit 49, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1HDQowi009391 ==============================End of - Headers==============================
OK, we have found, after extensive testing (over the last 15 years) that if you have an instrument that was bought after 1987 (when the oldest of ours, that we tested, was purchased) and it has only been modified by either the manufacturer or had ancillary equipment added to it by one of the XEDS companies or Gatan, or the like, that there is absolutely MINIMAL leakage of radiation from the instruments. For the past 15 years we have had radiation badges located permanently next to the microscopes right where the operator sits. these badges are developed every three months and replaced. The three oldest sets of badges, one on our JEOL 2010F (was on the JEOL 2000FX when we had it), one on our FEI SEM and one in my office show the following exposures:
Last 3 Months Year to Date (July to June) Lifetime (Since 1995) JEOL 2010F {10mrem {10mrem 78mrem FEI XL30FEG {10mrem {10mrem 133mrem Office Monitor {10mrem {10mrem 21 mrem
These numbers are from Landauer (www.landauerinc.com) and are listed as "Dose Equivalent" Office monitor is line of site to one of the few windows in the basement, assume it is cosmic ray readings that make it higher, but that is just a guess. Annual exposure limits, according to Landauer, are 5,000mrem for the body and organs and 50,000 for extremities and skin. General Public should expect to see 100mrem/year
So, as far as I can see, our sensors have indicated that we are getting about the level that Jo and Joe Average see in the streets. We still encourage anyone who is worried, or thinks they might be pregnant, or wanting to become pregnant, to get and wear a dosimeter when ever operating the instruments. We do not mandate dosimeters, neither does the University or the State of Michigan.
Hope this helps.
-- John Mansfield PhD Cphys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 15, 20 -- From jfmjfm-at-umich.edu Thu Feb 17 09:20:32 2011 15, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 15, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1HFKU0w029027 15, 20 -- for {microscopy-at-microscopy.com} ; Thu, 17 Feb 2011 09:20:32 -0600 15, 20 -- Received: FROM jfm-mac.engin.umich.edu (jfm-mac.engin.umich.edu [141.213.21.60]) 15, 20 -- By hellskitchen.mr.itd.umich.edu ID 4D5D3CBB.B2CE8.9494 ; 15, 20 -- Authuser jfmjfm; 15, 20 -- 17 Feb 2011 10:20:27 EST 15, 20 -- Subject: Re: [Microscopy] Re: EM radiation 15, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) 15, 20 -- Content-Type: text/plain; charset=iso-8859-1 15, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 15, 20 -- In-Reply-To: {201102171337.p1HDbKVe023600-at-ns.microscopy.com} 15, 20 -- Date: Thu, 17 Feb 2011 10:20:27 -0500 15, 20 -- Message-Id: {5A3A1F37-9441-4585-B1DE-7C6C92EAFA35-at-umich.edu} 15, 20 -- References: {201102171337.p1HDbKVe023600-at-ns.microscopy.com} 15, 20 -- To: microscopy-at-microscopy.com 15, 20 -- X-Mailer: Apple Mail (2.1082) 15, 20 -- Content-Transfer-Encoding: 8bit 15, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1HFKU0w029027 ==============================End of - Headers==============================
Ah yes! With all due respect, and no sarcasm intended, because I am a firm believer in "this method DID work in my hands".
However, of the following I am reminded at every mention of 'oil' and 'objective'.
In the late 1960's, one of my professors purchased a new Leitz Ortholux with Orthomat camera and fluorescence. On the day that the sale's person had finished setting the scope up, he and the professor went to lunch. When they returned, the professor tried the instrument and could not see a thing when he brought the oil immersion objective into play. After brief discussion the sale's person unscrewed the objective and inverted it to check the face of the lens. As he peered thru the reversed ocular, he felt something on his hand and discovered that the inverted objective was relieving its internals of a full load of what appeared to be immersion oil. It turned out that the professor's senior graduate student - having no experience with either advance English, translated German or research microscopes had sat at the scope and misunderstood what was meant by "oiling the oil immersion objective".
Since I understand how vexing the problem of a seized objective can be, I would only relate that in my 40 years of experience with such advanced microscopies as were available to me, I have never permitted 'creepy' oil to be placed near or on any part of the optical tube - except between the face lens of the oil immersion objective and the slide and between the slide and the upper element (when available) of the substage condenser.
Equivocation: I never intrude on another professional's methods as long as s/he is not using an instrument for which I have responsibility or is personal equipment. Thus, in the case of this suggestion by J. Ladd (with a preeminent last name), I would not presume to call his method wrong in any other person's hands but my own.
Cheers,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: jladd-at-temple.edu [mailto:jladd-at-temple.edu] Sent: Wednesday, February 16, 2011 5:44 PM To: Monson, Frederick
I couldn't resist saying, "This is a galling problem for any seized pair of nut and bolt much less an objective and a turret. To solve the problem, first one must understand its natural roots. (http://en.wikipedia.org/wiki/Galling)
Galling as it may be, this is a problem in metallurgy and/or materials science, and, ultimately, real nano and 'elemental' science.
In either case, the easiest remedy would appear to be a life-long schedule of periodic 'flexing' of the 'bolt' in the grasp of the 'nut', and, thus, prevent the, now understood, galling consequences.
In the case of a neglected microscope objective, when galled, the objective seized within the threads of the turret illustrates a grievous form of instrumental negligence - typical, I'm embarrassed to say, of far too many of us who use and care for them.
Harsh remedies are called for, and with a strip of hard rubber (1/16" thick) wrapped around the objective and in the grasp of an appropriate pair of pliers (long handles for leverage), the turret immobilized by the left hand, and finally, the pliers (in the right!) drawn 'gently' (but inexorably) toward the operator, the objective generally succumbs to the force and will be freed. The rubber should prevent the teeth of the jaws of the pliers from 'reaching' the metal of the objective, but commodiously provide for sufficient 'grip' that the rubber can substitute as the wrench. I purchased mine in the plumbing section at Home Depot
Hopeful that the previous will be helpful to many, I have become, finally, 'hic finis est'.
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: chaueter-at-bcm.edu [mailto:chaueter-at-bcm.edu] Sent: Wednesday, February 16, 2011 10:53 AM To: Monson, Frederick
I had similar problem before. A Zeiss scope guy shared me a tip- wear lab gloves to get a better grip on the objective while loosening it from the turret.
Claire
Claire Haueter Electron Microscopy Technologist Jan and Dan Duncan Neurological Research Institute Howard Hughes Medical Institute 1250 Moursund Street, Suite 1125 Houston, TX 77030 Lab Phone: (832) 824-8772 EMAIL: chaueter-at-bcm.edu
________________________________________ X-from: david.knecht-at-uconn.edu [david.knecht-at-uconn.edu] Sent: Wednesday, February 16, 2011 8:01 AM To: Haueter, Claire Menoza
What tricks/tools do people suggest for unscrewing objectives from the turret when you cannot loosen them by hand? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 24 -- From david.knecht-at-uconn.edu Wed Feb 16 07:51:02 2011 5, 24 -- Received: from mta31.charter.net (mta31.charter.net [216.33.127.82]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GDp1m4024520 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:51:01 -0600 5, 24 -- Received: from imp10 ([10.20.200.15]) by mta31.charter.net 5, 24 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP 5, 24 -- id {20110216135100.PQHH12685.mta31.charter.net-at-imp10} 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- Received: from [10.0.1.4] ([71.80.125.187]) 5, 24 -- by imp10 with smtp.charter.net 5, 24 -- id 8dqy1g00X42ikRj05dr0nv; Wed, 16 Feb 2011 08:51:00 -0500 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=kj9zAlcOel0A:10 a=O1RQMTG4XG3sO61s4HkA:9 5, 24 -- a=JK2UMX8g5W29FKWeE3o3bDKwXQ4A:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 5, 24 -- a=Rb0d9Ftq5AQA:10 5, 24 -- From: David Knecht charter {david.knecht-at-uconn.edu} 5, 24 -- Content-Type: text/plain; charset=us-ascii 5, 24 -- Subject: Removing stuck objectives 5, 24 -- Date: Wed, 16 Feb 2011 08:50:58 -0500 5, 24 -- Message-Id: {161CAA81-7F7F-4752-B540-FE389BB48246-at-uconn.edu} 5, 24 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 24 -- X-Mailer: Apple Mail (2.1082) 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GDp1m4024520 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 33 -- From chaueter-at-bcm.edu Wed Feb 16 09:46:42 2011 11, 33 -- Received: from iron3.corp.bcm.tmc.edu (iron3.corp.bcm.tmc.edu [128.249.224.15]) 11, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GFkg72011445 11, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 09:46:42 -0600 11, 33 -- X-IronPort-AV: E=Sophos;i="4.60,480,1291615200"; 11, 33 -- d="scan'208";a="81243051" 11, 33 -- Received: from unknown (HELO EPW-EXHUB4-P023.ad.bcm.edu) ([10.22.132.90]) 11, 33 -- by iron3-out.bcm.edu with ESMTP; 16 Feb 2011 09:46:41 -0600 11, 33 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 11, 33 -- EPW-EXHUB4-P023.ad.bcm.edu ([::1]) with mapi; Wed, 16 Feb 2011 09:46:41 -0600 11, 33 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 11, 33 -- To: "david.knecht-at-uconn.edu" {david.knecht-at-uconn.edu} , 11, 33 -- "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 33 -- Date: Wed, 16 Feb 2011 09:46:40 -0600 11, 33 -- Subject: RE: [Microscopy] Removing stuck objectives 11, 33 -- Thread-Topic: [Microscopy] Removing stuck objectives 11, 33 -- Thread-Index: AcvN4hQ5iILgBVt3Qmuq3zbW0F4Q+AADcc1q 11, 33 -- Message-ID: {F3EEF3767DFF3244826E72285C1C273448FB731155-at-EXCMSMBX03.ad.bcm.edu} 11, 33 -- References: {201102161401.p1GE1wkC007107-at-ns.microscopy.com} 11, 33 -- In-Reply-To: {201102161401.p1GE1wkC007107-at-ns.microscopy.com} 11, 33 -- Accept-Language: en-US 11, 33 -- Content-Language: en-US 11, 33 -- X-MS-Has-Attach: 11, 33 -- X-MS-TNEF-Correlator: 11, 33 -- acceptlanguage: en-US 11, 33 -- x-tm-as-product-ver: SMEX-8.0.0.1307-6.500.1024-17960.000 11, 33 -- x-tm-as-result: No--44.299700-8.000000-31 11, 33 -- x-tm-as-user-approved-sender: No 11, 33 -- x-tm-as-user-blocked-sender: No 11, 33 -- Content-Type: text/plain; charset="us-ascii" 11, 33 -- MIME-Version: 1.0 11, 33 -- Content-Transfer-Encoding: 8bit 11, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GFkg72011445 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 36 -- From FMonson-at-wcupa.edu Wed Feb 16 15:48:14 2011 28, 36 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) 28, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GLmErw020971 28, 36 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 15:48:14 -0600 28, 36 -- X-ASG-Debug-ID: 1297892893-0368bc101d1bb010001-4CH8be 28, 36 -- Received: from WCU-XCH-05.PASSHE.LCL (wcu-xch-05.wcupa.edu [144.26.0.89]) by MX01.WCUPA.EDU with ESMTP id tLCTAdhFXCImKvB4 (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 16:48:13 -0500 (EST) 28, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 28, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.89 28, 36 -- X-ASG-Whitelist: Client 28, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 28, 36 -- WCU-XCH-05.PASSHE.LCL ([fe80::8068:be0d:980f:1540%16]) with mapi id 28, 36 -- 14.01.0270.001; Wed, 16 Feb 2011 16:48:13 -0500 28, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 28, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 28, 36 -- Subject: RE: [Microscopy] RE: Removing stuck objectives 28, 36 -- Thread-Topic: [Microscopy] RE: Removing stuck objectives 28, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: Removing stuck objectives 28, 36 -- Thread-Index: AQHLzfGjDNkrdKfUJk2+UR6HjeXI1JQEo2RA 28, 36 -- Date: Wed, 16 Feb 2011 21:48:11 +0000 28, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C02256A-at-WCU-XCH-03.PASSHE.LCL} 28, 36 -- References: {201102161553.p1GFrJrv024390-at-ns.microscopy.com} 28, 36 -- In-Reply-To: {201102161553.p1GFrJrv024390-at-ns.microscopy.com} 28, 36 -- Accept-Language: en-US 28, 36 -- Content-Language: en-US 28, 36 -- X-MS-Has-Attach: 28, 36 -- X-MS-TNEF-Correlator: 28, 36 -- x-originating-ip: [10.28.57.79] 28, 36 -- Content-Type: text/plain; charset="us-ascii" 28, 36 -- MIME-Version: 1.0 28, 36 -- X-Barracuda-Connect: wcu-xch-05.wcupa.edu[144.26.0.89] 28, 36 -- X-Barracuda-Start-Time: 1297892893 28, 36 -- X-Barracuda-Encrypted: AES128-SHA 28, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 28, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 28, 36 -- Content-Transfer-Encoding: 8bit 28, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GLmErw020971 ==============================End of - Headers==============================
I'd like to add x-ray capabilities to our XL-30 SEM. As we already have an electron microprobe, I'm looking for a basic SDD system for mineral identification. If anyone has any recommendations (or non-recommendations) they would like to share I'd love to hear them. I'd be happy to hear form vendors too. Thanks for the help,
Glenn Poirier
Earth Science / Science de la Terre Research Services Canadian Museum of Nature Musée canadien de la nature t.613.364.4088 f.613.364.4027 www.nature.ca
==============================Original Headers============================== 8, 28 -- From GPoirier-at-mus-nature.ca Fri Feb 18 14:20:06 2011 8, 28 -- Received: from mail44.messagelabs.com (mail44.messagelabs.com [216.82.249.179]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p1IKK4vq013379 8, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Feb 2011 14:20:05 -0600 8, 28 -- X-VirusChecked: Checked 8, 28 -- X-Env-Sender: GPoirier-at-mus-nature.ca 8, 28 -- X-Msg-Ref: server-4.tower-44.messagelabs.com!1298060402!72508991!1 8, 28 -- X-StarScan-Version: 6.2.9; banners=-,-,- 8, 28 -- X-Originating-IP: [207.61.24.131] 8, 28 -- Received: (qmail 27173 invoked from network); 18 Feb 2011 20:20:02 -0000 8, 28 -- Received: from mus-nature.ca (HELO n-exchange.mus-nature.ca) (207.61.24.131) 8, 28 -- by server-4.tower-44.messagelabs.com with SMTP; 18 Feb 2011 20:20:02 -0000 8, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 28 -- Content-class: urn:content-classes:message 8, 28 -- MIME-Version: 1.0 8, 28 -- Content-Type: text/plain; 8, 28 -- charset="iso-8859-1" 8, 28 -- Subject: basic SDD for SEM 8, 28 -- Date: Fri, 18 Feb 2011 15:20:02 -0500 8, 28 -- Message-ID: {D225C9F115E35C489EF3C351749CD3360174D2D0-at-n-exchange.mus-nature.ca} 8, 28 -- X-MS-Has-Attach: 8, 28 -- X-MS-TNEF-Correlator: 8, 28 -- Thread-Topic: basic SDD for SEM 8, 28 -- Thread-Index: AcvPqTq2Iao5EGhHQgOvorewjy2OoA== 8, 28 -- From: "Glenn Poirier" {GPoirier-at-mus-nature.ca} 8, 28 -- To: {Microscopy-at-microscopy.com} 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1IKK4vq013379 ==============================End of - Headers==============================
Dear All, has anybody tried to programm a pc-based user-interface to move the 6 inch motorized stage of a Philips 525 SEM? I would be very fond to have a programm where I can set up the X, Y and rotation movement on the stage and save these instructions like in a timeline window for later automatic "replay". At least a small programm would be good to send stage positions and rotation values to the stage, for a first step... I have all the infos on the hardware and the stage software as PDFs,if needed.
Best wishes, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I want to thank you all for your suggestions and report back. I put WD-40 on the threads with a plastic q-tip, waited a while, then used a small strap wrench (Harbor Freight Tools) and I was able to get them all loose. Thanks- Dave
On Feb 16, 2011, at 1:48 PM, Louis Kerr wrote:
} I have used soft jaw pliers for this type of thing. I remove the other objectives and then use the least amount of pressure necessary and don't try too hard! I bought the pliers from this source: } http://www.garrettwade.com/slip-joint-soft-jaw-pliers/p/94P04.01/ } } For some stuff I have taken the locked together items (no objectives) to our machine shop and they tighten the outer item in the proper sized colletchuck in the metal lathe. They work it free by hand and simply use the lathe as a stationary method to hold the items. } } Hope it helps, } Louie } } From: "david knecht" {david.knecht-at-uconn.edu} } To: lkerr-at-mbl.edu } Sent: Wednesday, February 16, 2011 8:52:05 AM } Subject: [Microscopy] Removing stuck objectives } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } What tricks/tools do people suggest for unscrewing objectives from the turret when you cannot loosen them by hand? Thanks- Dave } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } } } } } ==============================Original Headers============================== } 5, 24 -- From david.knecht-at-uconn.edu Wed Feb 16 07:51:02 2011 } 5, 24 -- Received: from mta31.charter.net (mta31.charter.net [216.33.127.82]) } 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1GDp1m4024520 } 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 07:51:01 -0600 } 5, 24 -- Received: from imp10 ([10.20.200.15]) by mta31.charter.net } 5, 24 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP } 5, 24 -- id {20110216135100.PQHH12685.mta31.charter.net-at-imp10} } 5, 24 -- for {microscopy-at-microscopy.com} ; Wed, 16 Feb 2011 08:51:00 -0500 } 5, 24 -- Received: from [10.0.1.4] ([71.80.125.187]) } 5, 24 -- by imp10 with smtp.charter.net } 5, 24 -- id 8dqy1g00X42ikRj05dr0nv; Wed, 16 Feb 2011 08:51:00 -0500 } 5, 24 -- X-Authority-Analysis: v=1.0 c=1 a=kj9zAlcOel0A:10 a=O1RQMTG4XG3sO61s4HkA:9 } 5, 24 -- a=JK2UMX8g5W29FKWeE3o3bDKwXQ4A:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 } 5, 24 -- a=Rb0d9Ftq5AQA:10 } 5, 24 -- From: David Knecht charter {david.knecht-at-uconn.edu} } 5, 24 -- Content-Type: text/plain; charset=us-ascii } 5, 24 -- Subject: Removing stuck objectives } 5, 24 -- Date: Wed, 16 Feb 2011 08:50:58 -0500 } 5, 24 -- Message-Id: {161CAA81-7F7F-4752-B540-FE389BB48246-at-uconn.edu} } 5, 24 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 5, 24 -- Mime-Version: 1.0 (Apple Message framework v1082) } 5, 24 -- X-Mailer: Apple Mail (2.1082) } 5, 24 -- Content-Transfer-Encoding: 8bit } 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1GDp1m4024520 } ==============================End of - Headers============================== } } } -- } Louis Kerr } lkerr-at-mbl.edu } } Research and Education Support Coordinator } Marine Biological Laboratory } 7 MBL Street } Woods Hole, MA 02543 } 508-289-7273 } 508-540-6902 (FAX) } 508-292-0289 (Cell phone) } } VISIT OUR WEB SITES: } www.mbl.edu } www.courses.mbl.edu } }
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 6, 30 -- From david.knecht-at-uconn.edu Sat Feb 19 15:05:59 2011 6, 30 -- Received: from mta21.charter.net (mta21.charter.net [216.33.127.81]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1JL5xgf027011 6, 30 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 15:05:59 -0600 6, 30 -- Received: from imp09 ([10.20.200.9]) by mta21.charter.net 6, 30 -- (InterMail vM.7.09.02.04 201-2219-117-106-20090629) with ESMTP 6, 30 -- id {20110219210558.IDZL3705.mta21.charter.net-at-imp09} 6, 30 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 16:05:58 -0500 6, 30 -- Received: from [10.0.1.4] ([71.80.125.187]) 6, 30 -- by imp09 with smtp.charter.net 6, 30 -- id 9x5w1g00K42ikRj05x5yyb; Sat, 19 Feb 2011 16:05:58 -0500 6, 30 -- X-Authority-Analysis: v=1.0 c=1 a=2O3BFwOpmIwA:10 a=kj9zAlcOel0A:10 6, 30 -- a=fmRo681FAAAA:8 a=Zx37jsudAAAA:8 a=hOpmn2quAAAA:8 a=XEQcwk9yAAAA:8 6, 30 -- a=tKJhRnGwqB2IF1indnYA:9 a=E42ci2a0kbsUnOEZbnwA:7 6, 30 -- a=aLfKwEOJ4X3TRrR1k--edNH2yaEA:4 a=CjuIK1q_8ugA:10 a=bhAyqjwEwP0A:10 6, 30 -- a=Rb0d9Ftq5AQA:10 a=MLjXOaEC43wA:10 a=hzkTCRmJD-AA:10 a=TFoOy3d0hvIA:10 6, 30 -- a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=QD2H72FBEjfIe1Bz:21 6, 30 -- a=p3uDYOz7aQkDg8gX:21 6, 30 -- Content-Type: text/plain; charset=us-ascii 6, 30 -- Mime-Version: 1.0 (Apple Message framework v1082) 6, 30 -- Subject: Re: [Microscopy] Removing stuck objectives 6, 30 -- From: David Knecht charter {david.knecht-at-uconn.edu} 6, 30 -- In-Reply-To: {580131820.277393.1297882099107.JavaMail.root-at-mail.mbl.edu} 6, 30 -- Date: Sat, 19 Feb 2011 16:05:56 -0500 6, 30 -- Message-Id: {F5FFD204-0091-4689-B783-26AA2E442983-at-uconn.edu} 6, 30 -- References: {580131820.277393.1297882099107.JavaMail.root-at-mail.mbl.edu} 6, 30 -- To: microscopy microscopy {microscopy-at-microscopy.com} 6, 30 -- X-Mailer: Apple Mail (2.1082) 6, 30 -- Content-Transfer-Encoding: 8bit 6, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1JL5xgf027011 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both susan.trant-at-viha.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: susan.trant-at-viha.ca Name: Sue Trant
Organization: Vancouver Island Health Authority
Title-Subject: [Filtered] Immunoflourescence
Message: For some time we have been experiencing an intermittent but frequent intraepidermal fishnet staining pattern with all immunoreactants. Normally this is diagnostic of pemphigus. It can be seen in burn patients. Pemphigus is a rare disease. This artifact staining is occuring in about 50% or more of our current DIF (direct immunoflourescence) cases where there is no history suggestive of pemphigus.
I have not changed any of our antibodies, antibody dilutions, wash solutions, transport media. The skin control slides are not staining with this pattern. The renal biopsies that are being stained at the same time are not showing this staining problem nor are the renal controls.
Does anyone have a suggestion as to why this is happening? What antibody manufacturer do others use?
Thanks in advance Sue Trant EM Technologist Vancouver Island Health Authority Victoria, British Columbia Canada
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dhorne-at-interchange.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sc-at-nanoct.com.ar as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sc-at-nanoct.com.ar Name: Silvio Cechet
Organization: NanoCT
Title-Subject: [Filtered] Zeiss EM 10 manuals
Message: Hi all, I rescued an old Zeiss Em 10. It is not working , but I found the operation instructions, care and maintenance. Unfortunately I didnÂ’t find any electronic and electricals manual. Can someone help me? Thanks in advance
Hi Silvio, I do have all the manuals in PDF in German language. Contact me offline. It would be best to know what kind of EM10 (A, B,C, CR) you have. I also have spare parts, if you need and can provide help attaching modern digital cameras including read-out of magnification and HV-values from the scope.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 20.02.11 00:29, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both sc-at-nanoct.com.ar as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: sc-at-nanoct.com.ar Name: Silvio Cechet } } Organization: NanoCT } } Title-Subject: [Filtered] Zeiss EM 10 manuals } } Message: Hi all, } I rescued an old Zeiss Em 10. It is not working , but I found the } operation instructions, care and maintenance. Unfortunately I didnÂ’t } find any electronic and electricals manual. Can someone help me? } Thanks in advance } } Silvio Cechet } } Login Host: 190.230.130.164 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 19 17:25:10 2011 } 9, 25 -- Received: from znl.com ([206.69.208.20]) } 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1JNPA6S016621 } 9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 17:25:10 -0600 } 9, 25 -- Received: from localhost (localhost [127.0.0.1]) } 9, 25 -- by znl.com (Postfix) with ESMTP id 7C307339E27 } 9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 17:25:10 -0600 (CST) } 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 9, 25 -- Received: from znl.com ([127.0.0.1]) } 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 9, 25 -- with ESMTP id un2hCdSdlF51 for {microscopy-at-microscopy.com} ; } 9, 25 -- Sat, 19 Feb 2011 17:25:10 -0600 (CST) } 9, 25 -- Received: from 192-168-1-9.tpgi.com.au (123-243-23-38.static.tpgi.com.au [123.243.23.38]) } 9, 25 -- by znl.com (Postfix) with ESMTPA id D5343339E20 } 9, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 17:25:08 -0600 (CST) } 9, 25 -- Message-ID: {4D605151.5010102-at-microscopy.com} } 9, 25 -- Date: Sun, 20 Feb 2011 10:25:05 +1100 } 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 9, 25 -- Reply-To: sc-at-nanoct.com.ar } 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 9, 25 -- MIME-Version: 1.0 } 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 25 -- Subject: viaWWW:Zeiss EM 10 manuals } 9, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 9, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 23 -- From stefan.diller-at-t-online.de Sun Feb 20 04:08:06 2011 7, 23 -- Received: from mailout06.t-online.de (mailout06.t-online.de [194.25.134.19]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1KA85xh012165 7, 23 -- for {microscopy-at-microscopy.com} ; Sun, 20 Feb 2011 04:08:05 -0600 7, 23 -- Received: from fwd22.aul.t-online.de (fwd22.aul.t-online.de ) 7, 23 -- by mailout06.t-online.de with smtp 7, 23 -- id 1Pr6Cx-0003Vm-2j; Sun, 20 Feb 2011 11:08:03 +0100 7, 23 -- Received: from mac-pro-6.speedport.ip (Tob7ciZrZhhjGnvZHgoHhULP+sns3vruWJ33dopJFTJEt+O-53ZvFQ0Os6yGOwgweL-at-[87.178.229.118]) by fwd22.aul.t-online.de 7, 23 -- with esmtp id 1Pr6Cn-0TuTpY0; Sun, 20 Feb 2011 11:07:53 +0100 7, 23 -- Message-ID: {4D60E7F8.3040409-at-t-online.de} 7, 23 -- Date: Sun, 20 Feb 2011 11:07:52 +0100 7, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 23 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 7, 23 -- MIME-Version: 1.0 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- Subject: Re: [Microscopy] viaWWW:Zeiss EM 10 manuals 7, 23 -- References: {201102192329.p1JNTjDp032183-at-ns.microscopy.com} 7, 23 -- In-Reply-To: {201102192329.p1JNTjDp032183-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 23 -- X-ID: Tob7ciZrZhhjGnvZHgoHhULP+sns3vruWJ33dopJFTJEt+O-53ZvFQ0Os6yGOwgweL 7, 23 -- X-TOI-MSGID: b19f3ea3-bb35-4457-ab8c-bbe4e14c1ab6 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1KA85xh012165 ==============================End of - Headers==============================
I have a rather technical question concerning TEM imaging.
In the weak phase object approximation (for high resolution imaging of thin biological specimens) one assumes that the back focal plane contains the Fourier transform of the exit wave. According to Fourier optics (e.g. in Goodman) this is only strictly true if the object is placed in the front focal plane (moving the image plane to infinity). Otherwise one has to deal with additional quadratic phase factors.
My question: How close to the front focal plane is the object plane in a properly aligned Tem (when imaging at about 1000 nm underfocus)? Is it close enough to approximate the diffraction pattern by a simple FT without extra phase factors? If I assume a magnification of about 50, then the lens law gives me a ratio of about 1.02 between the object distance and the focal length. Does that seem reasonable?
Philip
==============================Original Headers============================== 6, 28 -- From Philip.Koeck-at-ki.se Mon Feb 21 08:07:37 2011 6, 28 -- Received: from smtp5.ki.se (smtp5.ki.se [130.237.99.130]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1LE7bV2023316 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Feb 2011 08:07:37 -0600 6, 28 -- Received: from KIMSX12.user.ki.se (kimsx12.user.ki.se [130.229.20.29]) 6, 28 -- by smtp5.ki.se (Postfix) with ESMTP id 75E3B295A1B; 6, 28 -- Mon, 21 Feb 2011 15:07:36 +0100 (CET) 6, 28 -- Received: from KIMSX04.user.ki.se ([fe80::1ca8:f793:b35c:95f5]) by 6, 28 -- KIMSX12.user.ki.se ([fe80::302d:7e0a:5ffb:60db%16]) with mapi id 6, 28 -- 14.01.0270.001; Mon, 21 Feb 2011 15:07:35 +0100 6, 28 -- From: =?iso-8859-1?Q?Philip_K=F6ck?= {Philip.Koeck-at-ki.se} 6, 28 -- To: "3dem-at-ncmir.ucsd.edu" {3dem-at-ncmir.ucsd.edu} , 6, 28 -- "microscopy-at-microscopy.com" 6, 28 -- {microscopy-at-microscopy.com} 6, 28 -- Subject: TEM: object distance 6, 28 -- Thread-Topic: TEM: object distance 6, 28 -- Thread-Index: AcvR0KKZtqTISCRIRwWyJqlU/lxwWw== 6, 28 -- Date: Mon, 21 Feb 2011 14:07:14 +0000 6, 28 -- Message-ID: {C8D24D7E19AEE543942A826EE910B1C20F42EC71-at-KIMSX04.user.ki.se} 6, 28 -- Accept-Language: en-GB, sv-SE, en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- x-originating-ip: [130.237.109.49] 6, 28 -- Content-Type: text/plain; charset="iso-8859-1" 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1LE7bV2023316 ==============================End of - Headers==============================
SIA offers beam stop for side mounted TEM cameras as part of SIA TEM camera systems or as a stand-alone upgrade. For all JEOL, Hitachi, and Zeiss models.
(Philips/FEI TEMs have factory-installed beam stop above side port)
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 2/19/2011 6:25 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both dhorne-at-interchange.ubc.ca as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: dhorne-at-interchange.ubc.ca Name: Derrick Horne } } Organization: UBC BioImaging Facility } } Title-Subject: [Filtered] Hitachi H7600 beam stop for CCD Camera } } Message: I'm looking for a beam stop to use with our Hitachi H7600 and a } side mount AMT camera. } } Might anyone have one with which they are willing to part? } } Thanks in advance } } Derrick } } Login Host: 137.82.85.211 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 19 17:24:32 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1JNOWHE014317 } 11, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 17:24:32 -0600 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id D10CA339E1F } 11, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 17:24:32 -0600 (CST) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 25 -- with ESMTP id cHTo+IVUGY46 for {microscopy-at-microscopy.com} ; } 11, 25 -- Sat, 19 Feb 2011 17:24:32 -0600 (CST) } 11, 25 -- Received: from 192-168-1-9.tpgi.com.au (123-243-23-38.static.tpgi.com.au [123.243.23.38]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id 312D3339E18 } 11, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Feb 2011 17:24:31 -0600 (CST) } 11, 25 -- Message-ID: {4D60512B.4050402-at-microscopy.com} } 11, 25 -- Date: Sun, 20 Feb 2011 10:24:27 +1100 } 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 11, 25 -- Reply-To: dhorne-at-interchange.ubc.ca } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: viaWWW:Hitachi H7600 beam stop for CCD Camera } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
I notice that you do not mention the salt concentrations in any of the fixation protocols you list. It is possible that by changing either the salt or the pH concentration, you could get some changes in the morphology of the vesicles. You might also leave out the poststain with UA, and see what that gets you. Joel
On Thu, Feb 10, 2011 at 3:13 PM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both shervin.esfahani-at-nih.gov as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: shervin.esfahani-at-nih.gov Name: Shervin Esfahani } } Title-Subject: [Filtered] Large Dense Core Vesicles } } Message: Good morning everyone, } I am currently trying to obtain good micrographs of LDCV's (large dense } core vesicles) but, have had a variety of issues when I go image them. } Ideally, the LDCV will have a distinct outer membrane, with a large, } dense and darkly stained interior. There is also a thin space between } the interior peptides and the membrane. (Hopefully this all makes sense } so far) I have been having issues where the contents inside the LDCV's } tend to shrink and shrivel up inside the membrane or, the entire LDCV is } stained so dark that there is no discernable membrane, and it resembles } a staining artifact. I have tried 4 different fixations (2.5% Glut in } Sodium Cacodylate, 4% Glut in Sodium Cacodylate, 1% Glut & 1% OsO4 in } Sodium Phosphate, and 2.5% Glut & 1% Paraformaldehyde in Sodium } Phosphate), each for 1 hr, followed by 1% OsO4 (except in the case of } the double fix), 1% Uranyl Acetate in cold/dark overnight, standard } ethanol dehydration, embedding in EMbed-812, and finally post section } staining with 1% Uranyl Acetate for 10 min (in dark), followed by Lead } Citrate staining for 2-3 min. Just wondering if anyone has any tips on } how to prevent this shrinking, or if there are any other tips on what } works best on these LDCV's. Thanks in advance! } Shervin G Esfahani } NIH/IRTA Fellow } NHLBI EM Core Facility } 9000 Rockville Pike } Bethesda, Maryland 20892 } 301.496.6448 } } Opinions and experiences related are those of Shervin Esfahani and do } not represent the NIH. This message is not confidential and can be } freely shared } and reproduced. } } } } Login Host: 156.40.232.65 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Feb 10 14:05:00 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1AK50PF001185 } 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 14:05:00 -0600 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 63AC432E0CD } 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 14:05:00 -0600 (CST) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id 0CeFL5GWXy9Z for {microscopy-at-microscopy.com} ; } 10, 25 -- Thu, 10 Feb 2011 14:04:58 -0600 (CST) } 10, 25 -- Received: from NestorPwrBk.local (nsit-dhcp-205-208-121-072.uchicago.edu [205.208.121.72]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id A52A032E0C2 } 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 10 Feb 2011 14:04:57 -0600 (CST) } 10, 25 -- Message-ID: {4D5444E5.3000208-at-microscopy.com} } 10, 25 -- Date: Fri, 11 Feb 2011 07:04:53 +1100 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: shervin.esfahani-at-nih.gov } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: viaWWW:Large Dense Core Vesicles } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Joel B. Sheffield, Ph.D. Biology Department, Temple University 1900 North 12th Street Philadelphia, PA 19122 jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486 http://astro.temple.edu/~jbs
==============================Original Headers============================== 9, 35 -- From joelsheffield-at-gmail.com Mon Feb 21 21:33:35 2011 9, 35 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1M3XX74017039 9, 35 -- for {microscopy-at-microscopy.com} ; Mon, 21 Feb 2011 21:33:34 -0600 9, 35 -- Received: by wwb17 with SMTP id 17so6900367wwb.18 9, 35 -- for {microscopy-at-microscopy.com} ; Mon, 21 Feb 2011 19:33:32 -0800 (PST) 9, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 35 -- d=gmail.com; s=gamma; 9, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 9, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 9, 35 -- bh=bX5eZVD+McnNE5GaNzCz2Qx9lcgTP0570+XZsDlCT/k=; 9, 35 -- b=Xn07MXeM5Dnj0IJz1X5UuSS1I1y06d1iZBK7RDV6O/6W9tVI1DGl68u/3RPXqnukhe 9, 35 -- NI3leaEr/ZTtNQqT3omFmbg2zO9OtYPb74effpInXmXxrNq/nMsrnSnqSRLWJLw5yuGf 9, 35 -- A+fLndfnDOMLzjPYe/c9V+Ap1iq5xtDXerxOo= 9, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 9, 35 -- d=gmail.com; s=gamma; 9, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 9, 35 -- :content-type:content-transfer-encoding; 9, 35 -- b=U4MGuO77kkkUM06iof26VscukAXCWBwm+ycAc4m6wUgo+eOmwkNo/2/4yLlqW2F9f6 9, 35 -- XyfW69MCOrL89DGsbDwCdwrXJe6c3buPVhlSkneTcdC+ManaxPd9VIsOBYP5sEhobCmo 9, 35 -- r5GBmw8fG6/cbe4rfv0XUvBZTdo9HLbmF+NnU= 9, 35 -- MIME-Version: 1.0 9, 35 -- Received: by 10.227.143.11 with SMTP id s11mr1864936wbu.21.1298345611772; Mon, 9, 35 -- 21 Feb 2011 19:33:31 -0800 (PST) 9, 35 -- Received: by 10.227.146.137 with HTTP; Mon, 21 Feb 2011 19:33:31 -0800 (PST) 9, 35 -- In-Reply-To: {201102102013.p1AKDXBt012477-at-ns.microscopy.com} 9, 35 -- References: {201102102013.p1AKDXBt012477-at-ns.microscopy.com} 9, 35 -- Date: Mon, 21 Feb 2011 22:33:31 -0500 9, 35 -- Message-ID: {AANLkTinqVdZcgQCcCYrZH6kFo+j2hEWrRdqGVccug6xW-at-mail.gmail.com} 9, 35 -- Subject: Re: [Microscopy] viaWWW:Large Dense Core Vesicles 9, 35 -- From: Joel Sheffield {joelsheffield-at-gmail.com} 9, 35 -- To: microscopy-at-microscopy.com 9, 35 -- Content-Type: text/plain; charset=ISO-8859-1 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1M3XX74017039 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both shm7-at-lehigh.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: shm7-at-lehigh.edu Name: Shuailei
Organization: CAMN, Lehigh University
Title-Subject: [Filtered] A Postdoctoral Position in Lehigh University
Message: A postdoctoral position is available immediately in the Center for Advanced Materials and Nanotechnology (CAMN) in Lehigh University. The successful candidate will focus on studying the grain boundary structure/chemistry in ceramics as well as metals using FIB, HRTEM, Cs-corrected STEM, EDS and EELS. The position requires a Ph. D. in materials science or related discipline. Hands on experience with aberration corrected STEM is desired and knowledge of FIB is preferred. This position is open until filled.
Applications with a cover letter and curriculum vitae should be sent to Prof. Martin P. Harmer Director, CAMN Lehigh University 5 E. Packer Ave. Bethlehem, PA 18015 mph2-at-lehigh.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ech-at-uvic.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ech-at-uvic.ca Name: Elaine Humphrey
Organization: University of Victoria
Title-Subject: [Filtered] fib gallium sources
Message: We are almost ready to replace our gallium source for our fib. The quote we have is double the last time we changed the source and we thought the last quote was big. It means the cost of using a fib must include the consumables which works out to be around Can$17/hr and when we change the source it will go up to Can$35/hr. The consumables being the LMIS source (the gallium), the GA aperture set, metal O ring and lower apertures.
When I checked on the web the price of gallium has almost doubled in the last year which is presumably why the consumables are going up.
Can the fib users let me know what their consumables cost and how much they charge their users please? Has anyone come up with a more reasonable solution to this problem? I'm not sure yet what my users will be willing to pay but this will be a shock to them.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] Nitrogen by EDS
Message: Greetings.
A colleague is attempting Nitrogen analysis by SEM/EDS and is having trouble detecting the N peak. Apparently no issue with O and C. I have little experience with N and was hoping others might have some helpful insight to any issues specific to N analysis?? Of course, I am assuming the problem isn't that his samples have no N to begin with!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both info-at-tescan-usa.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job Opening: TESCAN District Sales Manager, Midwest Region
Message: TESCAN, a leading manufacturer of Scanning Electron Microscopes and Focused Ion Beam Workstations established in 1991 is accepting resumes for a District Sales Manager in the Midwest region of the United States. Qualified candidates should be self-motivated, independent and a team player. A technical degree and background is required, experience with charge particle technology would be ideal. Candidates should possess excellent communication and organizational skills. All interested parties please submit resumes and salary requirements to info-at-tescan-usa.com. Previous sales experience is preferred but not necessarily required. Qualified candidates must be able to pass criminal, and credit background checks, including drug testing. A valid drivers License is required. TESCAN is an affirmative action/equal opportunity employer. Please limit all correspondence to info-at-tescan-usa.com.
Sensitivity to nitrogen by EDS is not great. Even with a low kV beam to reduce analysis depth and little carbon in the matrix, you must still contend with attenuation from the carbon present in most EDS thin windows.
To maximize detectability use a low kV beam (5 kV?) and tilt the sample to skew the analysis volume along the surface, minimizing penetration. A windowless or ultra thin detector window should be used. For most matrix compositions, standardless quantitation is pretty much useless under these conditions. Using a standard under similar operating conditions and no normalization will increase the odds of good quant.
Woody
N.W. (Woody) White Jr. Senior Electron Microscopist Chemistry and Materials Center AREVA NP Inc An AREVA and Siemens Company Lynchburg, VA Lab Phone: 434.832.3004
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, February 22, 2011 8:37 PM To: WHITE Norvell (RS/IB)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] Nitrogen by EDS
Message: Greetings.
A colleague is attempting Nitrogen analysis by SEM/EDS and is having trouble detecting the N peak. Apparently no issue with O and C. I have little experience with N and was hoping others might have some helpful insight to any issues specific to N analysis?? Of course, I am assuming the problem isn't that his samples have no N to begin with!
The C in the window of the detector absorbs the N K X-rays. Most detectors of this type can do B about as efficiently as N. There's not much you can do about it.
Lew Rabenberg
On Feb 22, 2011, at 7:24 PM, microscopylistserver- noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------- } ------ } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- http://www.microscopy.com/ } MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both tomw-at-uidaho.edu as well as the } MIcroscopy } Listserver } ---------------------------------------------------------------------- } ----- } } Email: tomw-at-uidaho.edu Name: Tom Williams } } Organization: University of Idaho } } Title-Subject: [Filtered] Nitrogen by EDS } } Message: Greetings. } } A colleague is attempting Nitrogen analysis by SEM/EDS and is having } trouble detecting the N peak. Apparently no issue with O and C. I } have } little experience with N and was hoping others might have some helpful } insight to any issues specific to N analysis?? Of course, I am } assuming } the problem isn't that his samples have no N to begin with! } } Any suggestions would be welcome and appreciated. } } Tom } } } Login Host: 76.178.153.103 } ---------------------------------------------------------------------- } ----- } } } } ==============================Original } Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb } 22 19:22:47 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id p1N1Mlb9008139 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Feb 2011 } 19:22:47 -0600 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 4C12A33DDF3 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Feb 2011 } 19:22:47 -0600 (CST) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 12, 25 -- with ESMTP id J9jh47JzDCuk for {microscopy-at-microscopy.com} ; } 12, 25 -- Tue, 22 Feb 2011 19:22:47 -0600 (CST) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 1E3F633DDE8 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Feb 2011 } 19:22:47 -0600 (CST) } 12, 25 -- Message-ID: {4D646166.4020807-at-microscopy.com} } 12, 25 -- Date: Tue, 22 Feb 2011 19:22:46 -0600 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 12, 25 -- Reply-To: tomw-at-uidaho.edu } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X } 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: [Filtered] viaWWW:Nitrogen by EDS } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 27 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 23 08:56:06 2011 9, 27 -- Received: from znl.com ([206.69.208.20]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1NEu6NC011245 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Feb 2011 08:56:06 -0600 9, 27 -- Received: from localhost (localhost [127.0.0.1]) 9, 27 -- by znl.com (Postfix) with ESMTP id 0AA7A33E9A3 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Feb 2011 08:56:06 -0600 (CST) 9, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 27 -- Received: from znl.com ([127.0.0.1]) 9, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 27 -- with ESMTP id 0jb7IoIjc3WY for {microscopy-at-microscopy.com} ; 9, 27 -- Wed, 23 Feb 2011 08:56:03 -0600 (CST) 9, 27 -- Received: from NestorPwrBk.local (mac21.zaluzec.com [206.69.208.21]) 9, 27 -- by znl.com (Postfix) with ESMTPA id 6EF4133E997 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Feb 2011 08:56:03 -0600 (CST) 9, 27 -- Message-ID: {4D652003.2060609-at-microscopy.com} 9, 27 -- Date: Thu, 24 Feb 2011 01:56:03 +1100 9, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 9, 27 -- Reply-To: Lew Rabenberg {lew.rabenberg-at-mail.utexas.edu} 9, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 27 -- MIME-Version: 1.0 9, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 27 -- Subject: Re: [Microscopy] viaWWW:Nitrogen by EDS 9, 27 -- References: {201102230124.p1N1OYoR014443-at-ns.microscopy.com} 9, 27 -- In-Reply-To: {201102230124.p1N1OYoR014443-at-ns.microscopy.com} 9, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Inorganic nitrogen is much easier to detect than organic nitrogen. There needs to be a lot (I can't quantify this, just a lot) of organic nitrogen present to detect with EDS. He could try running a standard of know nitrogen that would be comparable to his sample, if it exists. Running a Carbon, Hydrogen, Nitrogen analysis available in most chemistry departments would be a better way to determine nitrogen than EDS.
Emily McKimmy
On Tue, Feb 22, 2011 at 8:33 PM, { microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserver%0AOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: tomw-at-uidaho.edu Name: Tom Williams } } Organization: University of Idaho } } Title-Subject: [Filtered] Nitrogen by EDS } } Message: Greetings. } } A colleague is attempting Nitrogen analysis by SEM/EDS and is having } trouble detecting the N peak. Apparently no issue with O and C. I have } little experience with N and was hoping others might have some helpful } insight to any issues specific to N analysis?? Of course, I am assuming } the problem isn't that his samples have no N to begin with! } } Any suggestions would be welcome and appreciated. } } Tom } } } Login Host: 76.178.153.103 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Feb 22 } 19:22:47 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p1N1Mlb9008139 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Feb 2011 19:22:47 } -0600 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 4C12A33DDF3 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Feb 2011 19:22:47 } -0600 (CST) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 12, 25 -- with ESMTP id J9jh47JzDCuk for {microscopy-at-microscopy.com } } ; } 12, 25 -- Tue, 22 Feb 2011 19:22:47 -0600 (CST) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com[206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 1E3F633DDE8 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 22 Feb 2011 19:22:47 } -0600 (CST) } 12, 25 -- Message-ID: {4D646166.4020807-at-microscopy.com} } 12, 25 -- Date: Tue, 22 Feb 2011 19:22:46 -0600 } 12, 25 -- From: MicroscopyListserver-NoReply { } microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: tomw-at-uidaho.edu } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: [Filtered] viaWWW:Nitrogen by EDS } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 27 -- From microscopylistserver-noreply-at-microscopy.com Wed Feb 23 08:56:32 2011 5, 27 -- Received: from znl.com ([206.69.208.20]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1NEuW8e011748 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Feb 2011 08:56:32 -0600 5, 27 -- Received: from localhost (localhost [127.0.0.1]) 5, 27 -- by znl.com (Postfix) with ESMTP id 3E4D033E9B0 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Feb 2011 08:56:32 -0600 (CST) 5, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 5, 27 -- Received: from znl.com ([127.0.0.1]) 5, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 5, 27 -- with ESMTP id 4pFpBsGirHEn for {microscopy-at-microscopy.com} ; 5, 27 -- Wed, 23 Feb 2011 08:56:31 -0600 (CST) 5, 27 -- Received: from NestorPwrBk.local (mac21.zaluzec.com [206.69.208.21]) 5, 27 -- by znl.com (Postfix) with ESMTPA id CB5EE33E9A5 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Feb 2011 08:56:31 -0600 (CST) 5, 27 -- Message-ID: {4D65201F.5080000-at-microscopy.com} 5, 27 -- Date: Thu, 24 Feb 2011 01:56:31 +1100 5, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 5, 27 -- Reply-To: Emily McKimmy {mckimmye-at-gmail.com} 5, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 27 -- MIME-Version: 1.0 5, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 5, 27 -- Subject: Re: [Microscopy] viaWWW:Nitrogen by EDS 5, 27 -- References: {201102230133.p1N1X7q2007441-at-ns.microscopy.com} 5, 27 -- In-Reply-To: {201102230133.p1N1X7q2007441-at-ns.microscopy.com} 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The old edition of Goldstein et al. (which I stupidly, stupidly gave away!) had a nifty table of mass absorption coefficients in the Appendices. The value for carbon as an absorbent of N x-rays (as I recall) was something ridiculously high, like 25000. I had always assumed this was the root cause for not being able to see a nitrogen peak in organic samples. Am I wrong?
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both csuarez000-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: csuarez000-at-gmail.com Name: Anna
Organization: Heidelberg University
Title-Subject: [Filtered] cryosubstitution
Message: Dear Colleguaes;
I am working in viral morphogenisis focused into viral membranes. I would like if any of yours has experience in cryosubsitution of infected cells with acetone/methanol using like cryoprotectan saccharose/glycerol and if there are any incompatibility between them. Other people told me that the saccharose is precipitated during the cryosubstitution if you use methanol and the other possibility is to use methanol but this one is no good membrane preservatived. Thanks so much for your time
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin Foley
Organization: UAB
Title-Subject: [Filtered] X-Ray filament?
Message: All,
We're trying to find a tungsten filament for an syburg 160 kv microfocus x-ray tube. Syburg no longer exists. Does anyone know of someone who would retip a tungsten filament for an x-ray tube? Or, do you know of anyone that sells tungsten filaments for the tube. Note: this is a fancy x-ray tube that is turbo-pumped (not a static vacuum, replace the entire thing tube).
Dear Philip, Usually one puts the specimen at the eucentric height by tilting the stage, so that puts the specimen at the tilt axis, which may or may not be in the front focal plane. Since, when the stage is installed, its position is set by a mechanical adjustment, I think that the precision with which it can be aligned with the front focal plane is on the order of micrometers. Furthermore, the eucentric height is determined to a precision of tens of nanometers (or at least that's the best that I can do). Once the difference between the stage axis and the front focal plane has been measured, then one can position the specimen in that plane with a precision of tens of nm by setting the eucentric height, and then adjusting z to get to the front focal plane. I do not know whether one can easily measure the distance from the stage tilt axis to the front focal plane. I also do not know the effect of changing the objective lens current to image at a particular value of the defocus, although I expect it will shift the focal planes. A quick run through the formulas 1/I + 1/O = 1/f , and I/O = M does give ~1.02. Yours, Bill
X-from: Philip.Koeck-at-ki.se To: William.F.Tivol-at-aero.org
I have a rather technical question concerning TEM imaging.
In the weak phase object approximation (for high resolution imaging of thin biological specimens) one assumes that the back focal plane contains the Fourier transform of the exit wave. According to Fourier optics (e.g. in Goodman) this is only strictly true if the object is placed in the front focal plane (moving the image plane to infinity). Otherwise one has to deal with additional quadratic phase factors.
My question: How close to the front focal plane is the object plane in a properly aligned Tem (when imaging at about 1000 nm underfocus)? Is it close enough to approximate the diffraction pattern by a simple FT without extra phase factors? If I assume a magnification of about 50, then the lens law gives me a ratio of about 1.02 between the object distance and the focal length. Does that seem reasonable?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1OCQO07022572 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 24 Feb 2011 06:26:24 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p1OCQOxO022570; Thu, 24 Feb 2011 06:26:24 -0600
Elaine, such costs of FIB consumables seem to be enormously high. I just did a rough estimate for our FIB instruments (Tescan Lyra and Vela) - exchange of Ga source and column apertures by our US branch will be around 10000$, together with the serviceman transport and working time (assuming there is no service contract). Lifetime of our Ga source is longer than 1500 hours at 2 uA emission current, longer than 3000 hours at 1 uA emission current. Therefore in the worst case we obtain maximum ion beam costs less than 7$/hour, in the best case the running costs will be less than 4$/hour. I do not think that the price of Ga source was doubled because of doubled price of Ga on world markets. You need just negligible amount of Ga for one source. Definitely your supplier seems to be a sly businessman :-). Good luck, Tomas
} Email: ech-at-uvic.ca Name: Elaine Humphrey } } Organization: University of Victoria } } Title-Subject: [Filtered] fib gallium sources } } Message: We are almost ready to replace our gallium source for our } fib. The quote we have is double the last time we changed the source } and we thought the last quote was big. It means the cost of using a } fib must include the consumables which works out to be around Can } $17/hr and when we change the source it will go up to Can$35/hr. The } consumables being the LMIS source (the gallium), the GA aperture set, } metal O ring and lower apertures. } } When I checked on the web the price of gallium has almost doubled in } the last year which is presumably why the consumables are going up. } } Can the fib users let me know what their consumables cost and how much } they charge their users please? Has anyone come up with a more } reasonable solution to this problem? I'm not sure yet what my users } will be willing to pay but this will be a shock to them. } } many thanks } Elaine
==============================Original Headers============================== 4, 22 -- From tomas.hrncir-at-tescan.cz Thu Feb 24 06:26:24 2011 4, 22 -- Received: from mail.tescan.cz (mail.tescan.cz [80.83.67.110]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1OCQNnY022566 4, 22 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 06:26:23 -0600 4, 22 -- Received: from t51219 (bohumil.local [192.168.1.1]) 4, 22 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 22 -- (No client certificate requested) 4, 22 -- by mail.tescan.cz (Postfix) with ESMTP id CE96196809E; 4, 22 -- Thu, 24 Feb 2011 13:26:22 +0100 (CET) 4, 22 -- Date: Thu, 24 Feb 2011 13:26:22 +0100 4, 22 -- From: Tomas Hrncir {tomas.hrncir-at-tescan.cz} 4, 22 -- To: microscopy-at-microscopy.com 4, 22 -- Cc: ech-at-uvic.ca 4, 22 -- Subject: Re: [Microscopy] viaWWW:fib gallium sources 4, 22 -- Message-Id: {20110224132622.7d18f32d.tomas.hrncir-at-tescan.cz} 4, 22 -- In-Reply-To: {201102230139.p1N1dsNj030983-at-ns.microscopy.com} 4, 22 -- References: {201102230139.p1N1dsNj030983-at-ns.microscopy.com} 4, 22 -- Organization: Tescan 4, 22 -- X-Mailer: Sylpheed 3.1.0 (GTK+ 2.10.14; i686-pc-mingw32) 4, 22 -- Mime-Version: 1.0 4, 22 -- Content-Type: text/plain; charset=US-ASCII 4, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: UI
Title-Subject: [Filtered] N by EDS: Many Thanks
Message: Greeting all.
Wanted to take a moment to thank all the members for the useful suggestions and assistance with my Nitrogen question. I have forwarded the responses to my friend.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dhorne-at-interchange.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: With the aim of trying to maximise the use of our Hitachi S2600 VP-SEM, I am trying to source a used/refurbished cold stage and a chamberscope. I am partial to the Deben stages but am open to other choices.
I am even open to exploring the idea of having a student in Physics/Engineering build one for us if anyone has designs they are willing to share.
Please contact me offline with any offers/ideas/hints.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gv54-at-drexel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gv54-at-drexel.edu Name: Greg Vetterick
Organization: Drexel University
Title-Subject: [Filtered] Adhering Thin Films to Grids
Message: Hello all,
I'm currently trying to attach free standing 50nm-100nm metallic films to grids for TEM. My current method is to dip the grid in Gatan G-1 epoxy, wipe off the excess, then attach it to the film and cure.
My problem is that the viscosity of the G-1 epoxy is such that I often find that my grid spaces have been filled with epoxy.
Does anyone have experience thinning G-1 with a solvent? For a longer term fix, does anyone have suggestions for alternate epoxies or prep methods? I am intending to heat these in-situ ( {~600C).
When I attach Tripod Polished samples onto a grid, I use Epo-Tek 353ND epoxy that has very similar properties to G1. I use tabbed grids because they are easier to handle. I bend the tab a little so that when I hold them by the tab with tweezers, they will be held parallel to the horizontal. I put a small amount of epoxy on the grid using a very fine sable brush (000, I think) and then blot the excess off with filter paper that is lying flat under my stereomicroscope and holding the grid with the tweezers. I blot it off until I get a very thin layer. Then while still holding the tabbed grid with the tweezers, I touch the grid to the sample and try not to move it in a shearing motion. If your mesh isn't too high, this should work for you too. Different filter paper blots it differently.
I have also noticed that acetone can clean the uncured epoxy from stuff that I get it on accidently, so it might thin it for you too.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, February 24, 2011 5:24 PM To: swalck-at-southbaytech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gv54-at-drexel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gv54-at-drexel.edu Name: Greg Vetterick
Organization: Drexel University
Title-Subject: [Filtered] Adhering Thin Films to Grids
Message: Hello all,
I'm currently trying to attach free standing 50nm-100nm metallic films to grids for TEM. My current method is to dip the grid in Gatan G-1 epoxy, wipe off the excess, then attach it to the film and cure.
My problem is that the viscosity of the G-1 epoxy is such that I often find that my grid spaces have been filled with epoxy.
Does anyone have experience thinning G-1 with a solvent? For a longer term fix, does anyone have suggestions for alternate epoxies or prep methods? I am intending to heat these in-situ ( {~600C).
Why do you need epoxy? Have you tried just picking up the thin film on the grid? I've never needed to use anything to hold a electron-transparent thin film on a TEM grid. The van der Waals forces should hold it quite firmly.
Cheers, Henk
At 2/24/2011 8:17 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy bothgv54-at-drexel.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email:gv54-at-drexel.edu Name: Greg Vetterick } } Organization: Drexel University } } Title-Subject: [Filtered] Adhering Thin Films to Grids } } Message: Hello all, } } I'm currently trying to attach free standing 50nm-100nm metallic films } to grids for TEM. My current method is to dip the grid in Gatan G-1 } epoxy, wipe off the excess, then attach it to the film and cure. } } My problem is that the viscosity of the G-1 epoxy is such that I often } find that my grid spaces have been filled with epoxy. } } Does anyone have experience thinning G-1 with a solvent? } For a longer term fix, does anyone have suggestions for alternate } epoxies or prep methods? I am intending to heat these in-situ ( {~600C). } } Thank you in advance, } } -Greg } } } Login Host: 129.25.36.109 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 14, 25 -- Frommicroscopylistserver-noreply-at-microscopy.com Thu Feb 24 19:16:22 2011 } 14, 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1P1GLFD011098 } 14, 25 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 19:16:22 -0600 } 14, 25 -- Received: from localhost (localhost [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id F1427340723 } 14, 25 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 19:16:21 -0600 (CST) } 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 14, 25 -- with ESMTP id T4NJPwDf4toG for {microscopy-at-microscopy.com} ; } 14, 25 -- Thu, 24 Feb 2011 19:16:21 -0600 (CST) } 14, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 14, 25 -- by znl.com (Postfix) with ESMTPA id 87A7D340718 } 14, 25 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 19:16:21 -0600 (CST) } 14, 25 -- Message-ID: {4D6702E5.1020804-at-microscopy.com} } 14, 25 -- Date: Thu, 24 Feb 2011 19:16:21 -0600 } 14, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 14, 25 -- Reply-To:gv54-at-drexel.edu } 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 14, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 25 -- Subject: viaWWW:Adhering Thin Films to Grids } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 6, 27 -- From colijn.1-at-osu.edu Thu Feb 24 19:44:44 2011 6, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1P1iis6024740 6, 27 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 19:44:44 -0600 6, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 6, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 6, 27 -- id {01NY7V0O3DS09FLBOE-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 6, 27 -- Thu, 24 Feb 2011 20:44:43 -0500 (EST) 6, 27 -- Received: from [192.168.1.104] 6, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 6, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 6, 27 -- with ESMTPA id {01NY7V0NJG3I9F43KS-at-ecr6.ohio-state.edu} for 6, 27 -- microscopy-at-microscopy.com; Thu, 24 Feb 2011 20:44:43 -0500 (EST) 6, 27 -- Date: Thu, 24 Feb 2011 20:44:42 -0500 6, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 6, 27 -- Subject: Re: [Microscopy] viaWWW:Adhering Thin Films to Grids 6, 27 -- In-reply-to: {201102250117.p1P1HBbo014189-at-ns.microscopy.com} 6, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 6, 27 -- To: microscopy-at-microscopy.com 6, 27 -- Message-id: {4D67098A.9050708-at-osu.edu} 6, 27 -- MIME-version: 1.0 6, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 6, 27 -- Content-transfer-encoding: 7bit 6, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) 6, 27 -- Gecko/20101207 Thunderbird/3.1.7 6, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 6, 27 -- References: {201102250117.p1P1HBbo014189-at-ns.microscopy.com} ==============================End of - Headers==============================
You could try M-BOND-610 available from various EM suppliers. It is quite thin (less viscous than water) and should not form a film between the grids. You might want to blot it if there is too much. Long time ago when we did large quantity of hand-preps, I was shown/taught to breathe/exhale on the wet epoxy to make it stay put and tacky, just one slow AHH... apparently the moisture helps, or it might just be therapeutic.
Also it can be completely removed with Acetone when uncured if you overdo the application. I use 10-15 sec at 135°C fast cure, but it will cure at lower temperatures.
Hope this helps,
Jerzy *************************************************** Jerzy Gazda Ph.D. Section Manager - TEM, FIB, SEM Cerium Laboratories LLC 5204 E. Ben White Blvd. MS-512 Austin, TX 78741
(512) 934-5185 vm
www.ceriumlabs.com
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, February 24, 2011 7:25 PM To: Gazda, Jerzy
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gv54-at-drexel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gv54-at-drexel.edu Name: Greg Vetterick
Organization: Drexel University
Title-Subject: [Filtered] Adhering Thin Films to Grids
Message: Hello all,
I'm currently trying to attach free standing 50nm-100nm metallic films to grids for TEM. My current method is to dip the grid in Gatan G-1 epoxy, wipe off the excess, then attach it to the film and cure.
My problem is that the viscosity of the G-1 epoxy is such that I often find that my grid spaces have been filled with epoxy.
Does anyone have experience thinning G-1 with a solvent? For a longer term fix, does anyone have suggestions for alternate epoxies or prep methods? I am intending to heat these in-situ ( {~600C).
Hello, There is a method for making "sticky grids". I have learned it from a book "Electron Microscopy in Molecular Biology", page 4, IRL Press, Oxford, Washington DC, 1987: "... Spread out acetone-cleaned grids on filter paper, place a drop of an adhesive solution on each and allow to dry. The solution is either 1% polybutene in xylen or it is made by dissolving the adhesive from 5 cm of transparent sticky tape in 10 ml of ethylene dichloride. ..."
We are making the adhesive solution by dissolving adhesive from 5 cm of Scotch Magic tape No. 810. (The green one). It is important to use a glass vial and a glass Pasteur pipette. It works well for carbon support films and Pt/C replicas.
I hope it might work for you, too.
Best regards Oldrich
------------------ Oldrich Benada Institute of Microbiology AS CR, v.v.i. Videnska 1083 Prague 4 Czech Republic
On Friday 25 of February 2011 02:17:59 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both gv54-at-drexel.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: gv54-at-drexel.edu Name: Greg Vetterick } } Organization: Drexel University } } Title-Subject: [Filtered] Adhering Thin Films to Grids } } Message: Hello all, } } I'm currently trying to attach free standing 50nm-100nm metallic films } to grids for TEM. My current method is to dip the grid in Gatan G-1 } epoxy, wipe off the excess, then attach it to the film and cure. } } My problem is that the viscosity of the G-1 epoxy is such that I often } find that my grid spaces have been filled with epoxy. } } Does anyone have experience thinning G-1 with a solvent? } For a longer term fix, does anyone have suggestions for alternate } epoxies or prep methods? I am intending to heat these in-situ ( {~600C). } } Thank you in advance, } } -Greg } } } Login Host: 129.25.36.109 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 14, 25 -- From } microscopylistserver-noreply-at-microscopy.com Thu Feb 24 19:16:22 2011 14, } 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1P1GLFD011098 14, 25 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb } 2011 19:16:22 -0600 14, 25 -- Received: from localhost (localhost } [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id F1427340723 } 14, 25 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 19:16:21 -0600 } (CST) 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port 10024) 14, 25 -- with ESMTP id T4NJPwDf4toG for } {microscopy-at-microscopy.com} ; 14, 25 -- Thu, 24 Feb 2011 19:16:21 -0600 } (CST) } 14, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) 14, 25 -- by znl.com (Postfix) with ESMTPA id } 87A7D340718 } 14, 25 -- for {microscopy-at-microscopy.com} ; Thu, 24 Feb 2011 19:16:21 -0600 } (CST) 14, 25 -- Message-ID: {4D6702E5.1020804-at-microscopy.com} } 14, 25 -- Date: Thu, 24 Feb 2011 19:16:21 -0600 } 14, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} 14, 25 -- Reply-To: } gv54-at-drexel.edu } 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 14, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 25 -- Subject: viaWWW:Adhering Thin Films to Grids } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 23 -- From benada-at-biomed.cas.cz Fri Feb 25 01:43:32 2011 9, 23 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1P7hWpw032298 9, 23 -- for {microscopy-at-microscopy.com} ; Fri, 25 Feb 2011 01:43:32 -0600 9, 23 -- Received: from u117ob.localnet (a100ix.mbu.cas.cz [147.231.44.171]) 9, 23 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 23 -- (No client certificate requested) 9, 23 -- by mail2.biomed.cas.cz (Postfix) with ESMTP id 5C3EB1A44632; 9, 23 -- Fri, 25 Feb 2011 08:43:31 +0100 (CET) 9, 23 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 9, 23 -- Organization: =?utf-8?q?Mikrobiologck=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 9, 23 -- To: microscopy-at-microscopy.com 9, 23 -- Subject: Re: [Microscopy] viaWWW:Adhering Thin Films to Grids 9, 23 -- Date: Fri, 25 Feb 2011 08:43:19 +0100 9, 23 -- User-Agent: KMail/1.13.5 (Linux/2.6.32-5-686; KDE/4.4.5; i686; ; ) 9, 23 -- References: {201102250117.p1P1Hx3G016482-at-ns.microscopy.com} 9, 23 -- In-Reply-To: {201102250117.p1P1Hx3G016482-at-ns.microscopy.com} 9, 23 -- Cc: gv54-at-drexel.edu 9, 23 -- MIME-Version: 1.0 9, 23 -- Content-Type: Text/Plain; 9, 23 -- charset="windows-1250" 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- Message-Id: {201102250843.19890.benada-at-biomed.cas.cz} ==============================End of - Headers==============================
Dear All, anybody out there who has the Operating Instructions and the Technical Manual of the Jeol 840 as PDFs? I would be very fond of getting a copy.
Thanks, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
to this topic raised by Greg from Drexel University
} Title-Subject: [Filtered] Adhering Thin Films to Grids
} I'm currently trying to attach free standing 50nm-100nm metallic films } to grids for TEM. My current method is to dip the grid in Gatan G-1 } epoxy, wipe off the excess, then attach it to the film and cure. } } My problem is that the viscosity of the G-1 epoxy is such that I often } find that my grid spaces have been filled with epoxy. } } Does anyone have experience thinning G-1 with a solvent? } For a longer term fix, does anyone have suggestions for alternate } epoxies or prep methods? I am intending to heat these in-situ ( {~600C).
As Henk wrote - why not picking it up directly? with or without glow-discharge?
for biological samples, we adher Carbon films (metallic, too) with a thickness of say 10 or 20 nm onto Copper grids (usually, 400 or even 600 mesh). Our current method (and this is done by many): evaporate a thin carbon layer onto freshly cleaved mica. Evaporation: either from a Carbon /graphite rod in a vacuum coater (with a high current), or by electron beam evaporation. This carbon film floats off on water, the water is then lowered (carefully), until the film is covering some 20 or 50 grids (depending on the size of the mica). Before we put the naked Copper grids into the water, we hydrophilize them by 20-secs-glow-discharge (clean, no chemistry; and convenient). - Finally, the copper grids with the carbon film are air-dried, often baked for 2 hours at 120 or 140°C. - Usually, the carbon film adhers nicely to the grids. for us, this works fine, and is fairly easy. (We do not use polybutene or other adhesive, as suggested by Oldrich). or you may try to attach your 50-100-nm-metallic-films onto carbon-coated grids, and the carbon provides some "stickyness"? or Formvar-coated films, again frequently used in biology? BTW: we also attach Pt/C-replicas (1.5nm), backed with Carbon (10 to 15 nm), directly from a water-surface onto hydrophilized (glow-discharge), otherwise uncoated, untreated copper grids (700 mesh) - since many years, with success. Air-dry (25°C). They stick. For ever.
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 7, 24 -- From reinhard.rachel-at-biologie.uni-regensburg.de Fri Feb 25 12:20:49 2011 7, 24 -- Received: from rrzmta5.uni-regensburg.de (rrzmta5.uni-regensburg.de [194.94.155.56]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1PIKkrq017488 7, 24 -- for {microscopy-at-microscopy.com} ; Fri, 25 Feb 2011 12:20:49 -0600 7, 24 -- Received: from rrzmta5.uni-regensburg.de (localhost [127.0.0.1]) 7, 24 -- by localhost (Postfix) with SMTP id 5E5D937C9 7, 24 -- for {microscopy-at-microscopy.com} ; Fri, 25 Feb 2011 19:20:44 +0100 (CET) 7, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 24 -- by rrzmta5.uni-regensburg.de (Postfix) with ESMTP id 42EF337C3 7, 24 -- for {microscopy-at-microscopy.com} ; Fri, 25 Feb 2011 19:20:43 +0100 (CET) 7, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 24 -- with Novell_GroupWise; Fri, 25 Feb 2011 19:20:45 +0100 7, 24 -- Message-Id: {4D6801100200005400020A59-at-gwsmtp1.uni-regensburg.de} 7, 24 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 7, 24 -- Date: Fri, 25 Feb 2011 19:20:48 +0100 7, 24 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 7, 24 -- To: {microscopy-at-microscopy.com} 7, 24 -- Subject: Adhering Thin Films to Grids 7, 24 -- References: {201102250116.p1P1GWxF011591-at-ns.microscopy.com} 7, 24 -- In-Reply-To: {201102250116.p1P1GWxF011591-at-ns.microscopy.com} 7, 24 -- Mime-Version: 1.0 7, 24 -- Content-Type: text/plain; charset=UTF-8 7, 24 -- Content-Transfer-Encoding: 8bit 7, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
My apologies for being slow to respond to this post but here are some observations which may help.
The back focal plane is the Fourier transform of the front focal plane. But this does not mean that you have to use those planes in order to get the Fourier transform. If the sample is fairly close to (but not in) the front focal plane, there will be a plane fairly close to (but not in) the back focal plane that is the Fourier transform of the exit wave from the sample.
So, whether the sample is in the front focal plane or not, the question is then, how do you locate the right plane?
The other thread to the story is related to the eucentric height. It is easy to set the sample to the eucentric height but not easy to know if the eucentric height is at the front focal plane. This is addressed in the work of one of my students long ago: 'Skew Thoughts on Parallelism' K. K. Christenson and J. A. Eades Ultramicroscopy 26 (1988) 113-132 That paper includes a recipe (using a crystalline sample) for finding the true back focal plane, and hence the true front focal plane and thus a means to set the eucentric height to match the front focal plane.
However, you may not wish to adjust the height of the eucentric height of your stage and you will come to no harm leaving it where it is. So set your sample to the eucentric height (front focal plane or not) and then find the plane which is the Fourier transform by spreading the illumination wide (at the sample). Find the place where the direct beam is as sharp as possible: that is where the diffraction pattern is the Fourier transform of the sample exit wave.
In fact, you can be even more flexible. As long as the beam is somewhat spread at the sample I think that the plane in which the incident beam is a sharp spot will be a Fourier transform. (I hope someone whose math is slicker than mine will confirm this.) The problem with doing things in this haphazard way is that you do not know the camera length. Those of us who work (or in my case worked) with crystals tend to have calibrations built in, but if you work with non-crystalline materials you will need to calibrate the camera length under the precise conditions you use.
-- ........... Alwyn Eades Department of Materials Science and Engineering Lehigh University 5 East Packer Avenue Bethlehem Pennsylvania 18015-3195 Phone 610 758 4231 Fax 610 758 4244 jae5-at-lehigh.edu
In reply to:
I have a rather technical question concerning TEM imaging.
In the weak phase object approximation (for high resolution imaging of thin biological specimens) one assumes that the back focal plane contains the Fourier transform of the exit wave. According to Fourier optics (e.g. in Goodman) this is only strictly true if the object is placed in the front focal plane (moving the image plane to infinity). Otherwise one has to deal with additional quadratic phase factors.
My question: How close to the front focal plane is the object plane in a properly aligned Tem (when imaging at about 1000 nm underfocus)? Is it close enough to approximate the diffraction pattern by a simple FT without extra phase factors? If I assume a magnification of about 50, then the lens law gives me a ratio of about 1.02 between the object distance and the focal length. Does that seem reasonable?
==============================Original Headers============================== 13, 22 -- From jae5-at-lehigh.edu Fri Feb 25 14:43:33 2011 13, 22 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 13, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1PKhXqw006721 13, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 25 Feb 2011 14:43:33 -0600 13, 22 -- Received: from [127.0.0.1] (Dyn055026.mat.Lehigh.EDU [128.180.55.26]) 13, 22 -- (authenticated bits=0) 13, 22 -- by rain.cc.lehigh.edu (8.14.4/8.14.4) with ESMTP id p1PKhOBc019475 13, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 13, 22 -- Fri, 25 Feb 2011 15:43:29 -0500 13, 22 -- Message-ID: {4D681475.1020001-at-lehigh.edu} 13, 22 -- Date: Fri, 25 Feb 2011 15:43:33 -0500 13, 22 -- From: Alwyn Eades {jae5-at-lehigh.edu} 13, 22 -- Organization: Lehigh University 13, 22 -- User-Agent: Thunderbird 2.0.0.24 (Windows/20100228) 13, 22 -- MIME-Version: 1.0 13, 22 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} , 13, 22 -- Philip.Koeck-at-ki.se 13, 22 -- Subject: TEM object distance etc 13, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 22 -- Content-Transfer-Encoding: 7bit 13, 22 -- X-Virus-Scanned: clamav-milter 0.97 at rain.cc.lehigh.edu 13, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j-chung-at-ti.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j-chung-at-ti.com Name: Jayhoon Chung
Organization: Texas Instruments
Title-Subject: [Filtered] Job Opening: Product Engineer at Texas Instruments in Dallas
Message: Location: Greater Dallas Area, Texas Group: ATD Degree Requirements: Master's degree in Electrical Engineering, Engineering Physics, Applied Physics, Materials Science, Computer Engineering This position offers exposure to multiple TI process development, design, business, and manufacturing groups and will give this engineer the ability to have a major impact on essential analog technologies and drive revenue for years to come. There is tremendous opportunity for career development and personal growth in this position. In this role, you'll be responsible for physical failure analysis and electrical failure analysis of advanced analog processes. Specific responsibilities include: - Provide quality FA data to ensure timely qualification of analog processes. - Work with lab technicians and engineers to use the best FA techniques for each job. - Effectively relate physical analysis to process integration, design, and test engineers. - Work closely with process integration, design, and test engineers to identify root cause and solutions to yield and reliability issues identified during development. The ideal candidate will have 0-1 years of experience. A strong understanding of failure analysis techniques of advanced analog circuits is required, along with the ability to perform independent physical/electrical failure analysis and identify root causes of various failure modes for advanced analog products. Strong understanding of various failure analysis techniques, semiconductor device physics, and semiconductor manufacturing processes is a must. Hands-on experience with analytical tools, including TEM, SEM, FIB, and EMMI/OBIRCH is desired. Experience using various device delayering techniques including mechanical polishing and wet chemical is a definite plus. Knowledge/ experience with electrical failure analysis using curve tracer is preferred. This engineer must work well within a team spanning multiple functions and organizations. Strong communication skills are necessary to effectively understand inputs and relate results to team members and management. Texas Instruments is an Equal Opportunity Employer.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin D Foley
Organization: UAB
Title-Subject: [Filtered] Hall of Shame - Student Users
Message: Question for university facility managers -
Yesterday, on my very old SEM, a student user accidentally engaged the lock on the tilt. When the stage would not tilt, he decided to force it and snapped off the 5 mm diameter steel rod that you move to tilt the stage. He REALLY had to force it to cause this to happen. I'm sort of wondering if he beat it with a hammer - but I don't see any signs of that sort of damage!
What response do all of you take when someone screws up like this? How do you prevent it?
Also, what kind of training do you give your students prior to letting them loose on an SEM? We're about to replace our SEM with a new one with lots of features. I'm trying to get a feel for what normal training practices are and what works and doesn't work in your facility. I've had two incidents similar to this in a year (steel valve bent at 45 degrees on sputter coater), and it seems like too many. I might need some improvements in my training methods!
When I was running a facility, I always tried to be patient with the perpetrators of such events. Even the best of us did dumb things when learning and this is part of their education - to become people who will not mess up in the future. If you control them too tightly, they will never learn. After all, science comes from people willing to do things that have not been done before.
In our lab one extreme case involved a student drilling a hole into the chamber of an SEM - while it was running.
The hard part of the whole deal is convincing those responsible for funding that this is OK. And convincing yourself that this is OK even when the instrument damaged is a new and expensive one.
Alwyn Eades
On 2/26/2011 11:37 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both rfoley-at-uab.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu Name: Robin D Foley } } Organization: UAB } } Title-Subject: [Filtered] Hall of Shame - Student Users } } Message: Question for university facility managers - } } Yesterday, on my very old SEM, a student user accidentally engaged the } lock on the tilt. When the stage would not tilt, he decided to force it } and snapped off the 5 mm diameter steel rod that you move to tilt the } stage. He REALLY had to force it to cause this to happen. I'm sort of } wondering if he beat it with a hammer - but I don't see any signs of } that sort of damage! } } What response do all of you take when someone screws up like this? How } do you prevent it? } } Also, what kind of training do you give your students prior to letting } them loose on an SEM? We're about to replace our SEM with a new one } with lots of features. I'm trying to get a feel for what normal } training practices are and what works and doesn't work in your facility. } I've had two incidents similar to this in a year (steel valve bent at } 45 degrees on sputter coater), and it seems like too many. I might need } some improvements in my training methods! } } Thanks, } } Robin Foley } } Login Host: 138.26.80.163 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 26 10:31:55 2011 } 13, 26 -- Received: from znl.com ([206.69.208.20]) } 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QGVtPO027237 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } 13, 26 -- Received: from localhost (localhost [127.0.0.1]) } 13, 26 -- by znl.com (Postfix) with ESMTP id 2C77F342902 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 (CST) } 13, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 26 -- Received: from znl.com ([127.0.0.1]) } 13, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 13, 26 -- with ESMTP id Prs6CKcsW5+r for {microscopy-at-microscopy.com} ; } 13, 26 -- Sat, 26 Feb 2011 10:31:54 -0600 (CST) } 13, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 26 -- by znl.com (Postfix) with ESMTPA id AD8713428F7 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:54 -0600 (CST) } 13, 26 -- Message-ID: {4D692AFA.3040603-at-microscopy.com} } 13, 26 -- Date: Sat, 26 Feb 2011 10:31:54 -0600 } 13, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 13, 26 -- Reply-To: rfoley-at-uab.edu } 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 26 -- MIME-Version: 1.0 } 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 26 -- Subject: [Filtered] MicroscopyListserverviaWWW:Question for university facility } 13, 26 -- managers - Student Users } 13, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 26 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- Alwyn Eades Lehigh University
==============================Original Headers============================== 8, 24 -- From jae5-at-lehigh.edu Sat Feb 26 11:06:26 2011 8, 24 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QH6QQE011470 8, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 11:06:26 -0600 8, 24 -- Received: from [192.168.0.199] (70.15.4.31.res-cmts.sm.ptd.net [70.15.4.31]) 8, 24 -- (authenticated bits=0) 8, 24 -- by rain.cc.lehigh.edu (8.14.4/8.14.4) with ESMTP id p1QH6Ha7031839 8, 24 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 12:06:25 -0500 8, 24 -- Message-ID: {4D693309.1030409-at-lehigh.edu} 8, 24 -- Date: Sat, 26 Feb 2011 12:06:17 -0500 8, 24 -- From: Alwyn Eades {jae5-at-lehigh.edu} 8, 24 -- Reply-To: jae5-at-lehigh.edu 8, 24 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 8, 24 -- MIME-Version: 1.0 8, 24 -- To: Microscopy-at-microscopy.com 8, 24 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW:Question for 8, 24 -- university facility 8, 24 -- References: {201102261637.p1QGbi2s005111-at-ns.microscopy.com} 8, 24 -- In-Reply-To: {201102261637.p1QGbi2s005111-at-ns.microscopy.com} 8, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- X-Virus-Scanned: clamav-milter 0.97 at rain.cc.lehigh.edu 8, 24 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I am trying to find a balance between usefulness and price in getting a new target for my Hummer II sputter coater. I am looking at the various compositions of targets, and there is a huge price difference between them. Would I be able to successfully use a Pt/Pd target instead of an Au/Pd target? What are the primary concerns- has anyone had experience using targets other than Au/Pd?
Thank you,
Justin A. Kraft
==============================Original Headers============================== 3, 37 -- From kraftpiano-at-gmail.com Sat Feb 26 11:42:04 2011 3, 37 -- Received: from mail-gx0-f169.google.com (mail-gx0-f169.google.com [209.85.161.169]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QHg2mv028020 3, 37 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 11:42:03 -0600 3, 37 -- Received: by gxk2 with SMTP id 2so1384627gxk.0 3, 37 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 09:42:00 -0800 (PST) 3, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 37 -- d=gmail.com; s=gamma; 3, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 3, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 3, 37 -- bh=eI7yekD7Eip6dIIHss7YV/zljpOxO+De/YwCMD1VWRU=; 3, 37 -- b=uejcrqG0ww/00Co+Evt0Pggx4UuLWvlnGBpyWTuT8Hx/Ldb+QVOQHOp20Sw9gAt5ur 3, 37 -- h2VlwGGJW6duQZjb+ZvbGL3TTPXkAUhy/mhaVo4oMeAb6QSYVq/yf08JIWiweiSJAb0O 3, 37 -- bsmLCIKrwwih/1/UIaAjJMvB+rBKpgkPeYbyM= 3, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 37 -- d=gmail.com; s=gamma; 3, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 3, 37 -- :to:mime-version:x-mailer; 3, 37 -- b=ifDLEz4BhJtiYBuzK4NpfxMqNtc+k+/gUnTrFxsepOxdwqRBeBcR9ppV2jH9cWAzgh 3, 37 -- UGJuH6/x+SnjXmF/WLaFCoCGzcq4iYet04FLYJKDNggC0nYhDK3uEK8gfD5p7b2DQSDG 3, 37 -- DK5ILji+UhBV01WA4W2x45JX+lXhKv/GQB+dk= 3, 37 -- Received: by 10.236.136.134 with SMTP id w6mr2900941yhi.84.1298741557642; 3, 37 -- Sat, 26 Feb 2011 09:32:37 -0800 (PST) 3, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 3, 37 -- by mx.google.com with ESMTPS id x16sm1135520yhe.24.2011.02.26.09.32.36 3, 37 -- (version=TLSv1/SSLv3 cipher=OTHER); 3, 37 -- Sat, 26 Feb 2011 09:32:36 -0800 (PST) 3, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 37 -- Content-Type: text/plain; charset=us-ascii 3, 37 -- Subject: SEM Prep: Sputter coater target material selection? 3, 37 -- Date: Sat, 26 Feb 2011 12:32:34 -0500 3, 37 -- Message-Id: {7B1BF823-41EE-4F52-9916-6CC6D033EF9B-at-gmail.com} 3, 37 -- To: microscopy-at-microscopy.com 3, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) 3, 37 -- X-Mailer: Apple Mail (2.1082) 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1QHg2mv028020 ==============================End of - Headers==============================
I agree with Alwyn that "doing dumb things" is part of the education. As facility directors/managers, however, I believe it is our job to teach/train our users to do as few "dumb things" as possible, especially when these "dumb things" have a big consequence. It is costly to repair, and prevents other people doing research (the other important mission of the facilities).
We have a written policy that if a student damages an instrument due to improper operation, the PI has to agree to pay for the repair (if any). Even education is not free and we all learned that part.
With that said, we always try to teach students beforehand, to avoid any unnecessary damages to the instrument. One thing we always tell the students is the "two fingers rule" - Nothing on the instrument (TEM, SEM, confocal) requires forces greater than what your two fingers can handle. If you cannot open/close anything with your two fingers, STOP. That works pretty well, in most of the circumstances.
Hope this helps,
Zhaojie
Zhaojie Zhang, director Jenkins Microscopy Facility University of Wyoming
________________________________________ X-from: jae5-at-lehigh.edu [jae5-at-lehigh.edu] Sent: Saturday, February 26, 2011 10:09 AM To: Z.J. Zhang
When I was running a facility, I always tried to be patient with the perpetrators of such events. Even the best of us did dumb things when learning and this is part of their education - to become people who will not mess up in the future. If you control them too tightly, they will never learn. After all, science comes from people willing to do things that have not been done before.
In our lab one extreme case involved a student drilling a hole into the chamber of an SEM - while it was running.
The hard part of the whole deal is convincing those responsible for funding that this is OK. And convincing yourself that this is OK even when the instrument damaged is a new and expensive one.
Alwyn Eades
On 2/26/2011 11:37 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both rfoley-at-uab.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu Name: Robin D Foley } } Organization: UAB } } Title-Subject: [Filtered] Hall of Shame - Student Users } } Message: Question for university facility managers - } } Yesterday, on my very old SEM, a student user accidentally engaged the } lock on the tilt. When the stage would not tilt, he decided to force it } and snapped off the 5 mm diameter steel rod that you move to tilt the } stage. He REALLY had to force it to cause this to happen. I'm sort of } wondering if he beat it with a hammer - but I don't see any signs of } that sort of damage! } } What response do all of you take when someone screws up like this? How } do you prevent it? } } Also, what kind of training do you give your students prior to letting } them loose on an SEM? We're about to replace our SEM with a new one } with lots of features. I'm trying to get a feel for what normal } training practices are and what works and doesn't work in your facility. } I've had two incidents similar to this in a year (steel valve bent at } 45 degrees on sputter coater), and it seems like too many. I might need } some improvements in my training methods! } } Thanks, } } Robin Foley } } Login Host: 138.26.80.163 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 26 10:31:55 2011 } 13, 26 -- Received: from znl.com ([206.69.208.20]) } 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QGVtPO027237 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } 13, 26 -- Received: from localhost (localhost [127.0.0.1]) } 13, 26 -- by znl.com (Postfix) with ESMTP id 2C77F342902 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 (CST) } 13, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 26 -- Received: from znl.com ([127.0.0.1]) } 13, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 13, 26 -- with ESMTP id Prs6CKcsW5+r for {microscopy-at-microscopy.com} ; } 13, 26 -- Sat, 26 Feb 2011 10:31:54 -0600 (CST) } 13, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 26 -- by znl.com (Postfix) with ESMTPA id AD8713428F7 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:54 -0600 (CST) } 13, 26 -- Message-ID: {4D692AFA.3040603-at-microscopy.com} } 13, 26 -- Date: Sat, 26 Feb 2011 10:31:54 -0600 } 13, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 13, 26 -- Reply-To: rfoley-at-uab.edu } 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 26 -- MIME-Version: 1.0 } 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 26 -- Subject: [Filtered] MicroscopyListserverviaWWW:Question for university facility } 13, 26 -- managers - Student Users } 13, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 26 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- Alwyn Eades Lehigh University
==============================Original Headers============================== 8, 24 -- From jae5-at-lehigh.edu Sat Feb 26 11:06:26 2011 8, 24 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 8, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QH6QQE011470 8, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 11:06:26 -0600 8, 24 -- Received: from [192.168.0.199] (70.15.4.31.res-cmts.sm.ptd.net [70.15.4.31]) 8, 24 -- (authenticated bits=0) 8, 24 -- by rain.cc.lehigh.edu (8.14.4/8.14.4) with ESMTP id p1QH6Ha7031839 8, 24 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 8, 24 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 12:06:25 -0500 8, 24 -- Message-ID: {4D693309.1030409-at-lehigh.edu} 8, 24 -- Date: Sat, 26 Feb 2011 12:06:17 -0500 8, 24 -- From: Alwyn Eades {jae5-at-lehigh.edu} 8, 24 -- Reply-To: jae5-at-lehigh.edu 8, 24 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 8, 24 -- MIME-Version: 1.0 8, 24 -- To: Microscopy-at-microscopy.com 8, 24 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW:Question for 8, 24 -- university facility 8, 24 -- References: {201102261637.p1QGbi2s005111-at-ns.microscopy.com} 8, 24 -- In-Reply-To: {201102261637.p1QGbi2s005111-at-ns.microscopy.com} 8, 24 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 24 -- Content-Transfer-Encoding: 7bit 8, 24 -- X-Virus-Scanned: clamav-milter 0.97 at rain.cc.lehigh.edu 8, 24 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 15, 29 -- From ZZhang-at-uwyo.edu Sat Feb 26 11:54:53 2011 15, 29 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32]) 15, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QHsr1S011670 15, 29 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 11:54:53 -0600 15, 29 -- Received: from ponyexpress-ht1.uwyo.edu (ponyexpress-ht1.uwyo.edu [10.84.60.208]) 15, 29 -- by aspensprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p1QHsfkM028935 15, 29 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); 15, 29 -- Sat, 26 Feb 2011 10:54:42 -0700 (MST) 15, 29 -- (envelope-from ZZhang-at-uwyo.edu) 15, 29 -- Received: from ponyexpress-mb2.uwyo.edu ([10.84.60.213]) by ponyexpress-ht1 15, 29 -- ([10.84.60.208]) with mapi; Sat, 26 Feb 2011 10:54:41 -0700 15, 29 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 15, 29 -- To: "jae5-at-lehigh.edu" {jae5-at-lehigh.edu} , 15, 29 -- "microscopy-at-microscopy.com" 15, 29 -- {microscopy-at-microscopy.com} 15, 29 -- Date: Sat, 26 Feb 2011 10:54:40 -0700 15, 29 -- Subject: RE: [Microscopy] MicroscopyListserverviaWWW:Question for 15, 29 -- Thread-Topic: [Microscopy] MicroscopyListserverviaWWW:Question for 15, 29 -- Thread-Index: AQHL1d49n2T/lfDZu0+GoW/xVGM96Q== 15, 29 -- Message-ID: {8E12868E4E3D65439AEC1BAAF2BF60BEE91BD72D-at-ponyexpress-mb2} 15, 29 -- Accept-Language: en-US 15, 29 -- Content-Language: en-US 15, 29 -- X-MS-Has-Attach: 15, 29 -- X-MS-TNEF-Correlator: 15, 29 -- acceptlanguage: en-US 15, 29 -- Content-Type: text/plain; charset="us-ascii" 15, 29 -- MIME-Version: 1.0 15, 29 -- Content-Transfer-Encoding: 8bit 15, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1QHsr1S011670 ==============================End of - Headers==============================
I was wondering who owns the rights to Boron Nitride EDS windows? Are they available by special order? We would much prefer to trade Carbon transmission for Nitrogen/Titanium transmission.
I remember that our old Kevex BN window was excellent in this regard.
thanks a lot!
bryan tracy Spansion, Sunnyvale
==============================Original Headers============================== 6, 31 -- From Bryan.Tracy-at-spansion.com Sat Feb 26 12:17:53 2011 6, 31 -- Received: from USSVLMGTY02.spansion.com (ussvlmgty02.spansion.com [12.151.36.113]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QIHrRc027935 6, 31 -- for {Microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 12:17:53 -0600 6, 31 -- Received: from ussvlvexcasht2.spansion.com ([10.247.35.124]) 6, 31 -- by USSVLMGTY02.spansion.com with ESMTP; 26 Feb 2011 10:17:52 -0800 6, 31 -- Received: from US2K7EXMBPF1.spansion.com ([10.247.27.4]) by 6, 31 -- USSVLVEXCASHT2.spansion.com ([10.247.35.124]) with mapi; Sat, 26 Feb 2011 6, 31 -- 10:17:52 -0800 6, 31 -- From: "Tracy, Bryan" {Bryan.Tracy-at-spansion.com} 6, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 31 -- Date: Sat, 26 Feb 2011 10:17:47 -0800 6, 31 -- Subject: Boron Nitride EDS Windows (available?) 6, 31 -- Thread-Topic: Boron Nitride EDS Windows (available?) 6, 31 -- Thread-Index: AcvV4Xi4k1PQ1C7UQLyPcAQ8Hc8BKg== 6, 31 -- Message-ID: {F946A2B523FFC24EABFA434A3D143C82052FB5AF41-at-US2K7EXMBPF1.spansion.com} 6, 31 -- Accept-Language: en-US 6, 31 -- Content-Language: en-US 6, 31 -- X-MS-Has-Attach: 6, 31 -- X-MS-TNEF-Correlator: 6, 31 -- x-cr-puzzleid: {E34DBEA0-AB23-4F55-9B74-3306E4D7DA1F} 6, 31 -- x-cr-hashedpuzzle: Af96 AgTK AiUS Bzsm DYPd Dy86 EFbM ETon Gj2a Gvgp G1Xg 6, 31 -- G7wJ IfHE IxLA IySK 6, 31 -- JTy2;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{E34DBEA0-AB23-4F55-9B74-3306E4D7DA1F};YgByAHkAYQBuAC4AdAByAGEAYwB5AEAAcwBwAGEAbgBzAGkAbwBuAC4AYwBvAG0A;Sat, 6, 31 -- 26 Feb 2011 18:17:48 6, 31 -- GMT;QgBvAHIAbwBuACAATgBpAHQAcgBpAGQAZQAgAEUARABTACAAVwBpAG4AZABvAHcAcwAgACAAKABhAHYAYQBpAGwAYQBiAGwAZQA/ACkA 6, 31 -- acceptlanguage: en-US 6, 31 -- Content-Type: text/plain; charset="us-ascii" 6, 31 -- MIME-Version: 1.0 6, 31 -- Content-Transfer-Encoding: 8bit 6, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1QIHrRc027935 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Michael.Cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Michael.Cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball Langone NYU Med Center
Title-Subject: [Filtered] RE: [Microscopy] Question for university facility
Message: All the examples given in this exchange so far, snapping the steel rod, bending a valve on a sputter coater, and drilling a hole in an SEM are cases of the intentional application of force. Do students really need to be told not to do this as part of training? Have we descended so low in our contemporary spoon-feeding educational system? Maybe so. Make this a part of the training.
I can list a few stupid mistakes I've made and many I've seen others make, but the actions reported here, no matter how destructive in their results, were the intentional application of brute force by novices who should have known better.
There was an exchange here a few weeks ago about how to unstick a locked objective. If the result after the exchange was a broken objective, it was done 1.) after substantial recommendations from experts who had successfully solved the same problem and 2.) by the expert who is supposed to maintain the instruments and as part of his/her job description takes on these risks/responsibilities.
No facility users should be reconfiguring software in the adminstrator account, rewiring devices, or applying crowbars to stuck metal parts. _________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
I have been teaching microscopy and managing/directing a good sized service/multi-user EM facility for over 20 years (additional time as a user or service provider in smaller facilities). I am very happy to say that in that period we have had extremely few user accidents. I attribute that to three major reasons: 1) good training for all users before they are given their flying license. 2) support by facility staff to assist, answer questions, continue to train/educate on a routine basis. 3) A certain amount of fear that users can loose access privileges if they misuse instruments/break rules/do not respect other users.
(1) requires offering (and requiring) courses where students learn basic theory and hands-on use of major instrumentation....during which they are instructed in both what to do and what not to do. If you are not willing to put in the time required to train/educate users than you and the instruments will run into problems at some point. It all starts from learning correctly in the beginning with the emphasis on good habits, sufficient understanding to make good choices, and thus get good results. Users training users is problematic in my experience, as bad habits get passed on and magnified over time.
2) A good relationship between facility staff and users results in users who are not hesitant to ask for help. This means knowing your users, showing an interest in them and their projects and offering assistance when asked. You will quickly learn who needs some additional help along the way and who can work truly independently.
3) I don't apologize for this one. Facility rules are there for a reason. Down instruments due to user error affect many and that is unfair. The primary rule I have always enforces is that users to do not attempt to fix anything at any time. What seems simple often is a sign of much more significant problems that can be averted if the staff knows about them and responds accordingly. Failure to follow this rule results in much closer scrutiny and potential loss of privileges (rarely necessary).
All users may not perform at the same level but that does not mean that all cannot learn to use instruments with respect for the instrument and their fellow users. Part of our jobs as facility staff is to teach, train, and support to insure that this happens. We may not be 100% successful, but the number and severity of incidents will decrease significantly as a result.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
On 2/26/11 11:33 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both rfoley-at-uab.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu Name: Robin D Foley } } Organization: UAB } } Title-Subject: [Filtered] Hall of Shame - Student Users } } Message: Question for university facility managers - } } Yesterday, on my very old SEM, a student user accidentally engaged the } lock on the tilt. When the stage would not tilt, he decided to force it } and snapped off the 5 mm diameter steel rod that you move to tilt the } stage. He REALLY had to force it to cause this to happen. I'm sort of } wondering if he beat it with a hammer - but I don't see any signs of } that sort of damage! } } What response do all of you take when someone screws up like this? How } do you prevent it? } } Also, what kind of training do you give your students prior to letting } them loose on an SEM? We're about to replace our SEM with a new one } with lots of features. I'm trying to get a feel for what normal } training practices are and what works and doesn't work in your facility. } I've had two incidents similar to this in a year (steel valve bent at } 45 degrees on sputter coater), and it seems like too many. I might need } some improvements in my training methods! } } Thanks, } } Robin Foley } } Login Host: 138.26.80.163 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 26 10:31:55 } 2011 } 13, 26 -- Received: from znl.com ([206.69.208.20]) } 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1QGVtPO027237 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } 13, 26 -- Received: from localhost (localhost [127.0.0.1]) } 13, 26 -- by znl.com (Postfix) with ESMTP id 2C77F342902 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } (CST) } 13, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 26 -- Received: from znl.com ([127.0.0.1]) } 13, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } 10024) } 13, 26 -- with ESMTP id Prs6CKcsW5+r for {microscopy-at-microscopy.com} ; } 13, 26 -- Sat, 26 Feb 2011 10:31:54 -0600 (CST) } 13, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 26 -- by znl.com (Postfix) with ESMTPA id AD8713428F7 } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:54 -0600 } (CST) } 13, 26 -- Message-ID: {4D692AFA.3040603-at-microscopy.com} } 13, 26 -- Date: Sat, 26 Feb 2011 10:31:54 -0600 } 13, 26 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 13, 26 -- Reply-To: rfoley-at-uab.edu } 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 26 -- MIME-Version: 1.0 } 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 26 -- Subject: [Filtered] MicroscopyListserverviaWWW:Question for } university facility } 13, 26 -- managers - Student Users } 13, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 26 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 15, 30 -- From dsherman-at-purdue.edu Sat Feb 26 15:31:30 2011 15, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QLVTPm030583 15, 30 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 15:31:29 -0600 15, 30 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 15, 30 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p1QLVTT9008710 15, 30 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 16:31:29 -0500 15, 30 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB02F.purdue.lcl 15, 30 -- ([::1]) with mapi; Sat, 26 Feb 2011 16:31:29 -0500 15, 30 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 15, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} , 15, 30 -- "rfoley-at-uab.edu" 15, 30 -- {rfoley-at-uab.edu} 15, 30 -- Date: Sat, 26 Feb 2011 16:31:27 -0500 15, 30 -- Subject: Re: [Microscopy] for university facility 15, 30 -- Thread-Topic: [Microscopy] for university facility 15, 30 -- Thread-Index: AcvV0uH/+4/aT9+DRJOYp4UA4HB25AAKaQy/ 15, 30 -- Message-ID: {C98EDB5F.C2C8%dsherman-at-purdue.edu} 15, 30 -- Accept-Language: en-US 15, 30 -- Content-Language: en-US 15, 30 -- X-MS-Has-Attach: 15, 30 -- X-MS-TNEF-Correlator: 15, 30 -- user-agent: Microsoft-Entourage/13.8.0.101117 15, 30 -- acceptlanguage: en-US 15, 30 -- Content-Type: text/plain; charset="us-ascii" 15, 30 -- MIME-Version: 1.0 15, 30 -- X-PMX-Version: 5.5.9.388399 15, 30 -- X-PerlMx-Virus-Scanned: Yes 15, 30 -- Content-Transfer-Encoding: 8bit 15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1QLVTPm030583 ==============================End of - Headers==============================
You have had a good set of responses and the importance of patience and practical, hands on, teaching cannot be overemphasised. One piece of advice I would give is never to bawl out your user no matter how 'stupid' they have been. They must be completely confident to come to you to explain exactly how the damage occurred, if they are scared of you they will hide the damage (if possible) or not admit what happened. You are then in a worse position of discovering the problem some time later and not knowing how it occurred.
Of course rules are important, particularly users not making modifications to operating proceedures, software or hardware without authorisation, and ultimately withdrawing the access rights of someone who blatently disregards rules must be used to protect the research programs of the other users.
Our remit as a university is education and research, everyone has to learn how to treat instrumentation properly and this will occasionally result in damage, use those occasions to educate.
Regards, Ron
Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: 26 February 2011 16:40 To: Ron Doole
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin D Foley
Organization: UAB
Title-Subject: [Filtered] Hall of Shame - Student Users
Message: Question for university facility managers -
Yesterday, on my very old SEM, a student user accidentally engaged the lock on the tilt. When the stage would not tilt, he decided to force it and snapped off the 5 mm diameter steel rod that you move to tilt the stage. He REALLY had to force it to cause this to happen. I'm sort of wondering if he beat it with a hammer - but I don't see any signs of that sort of damage!
What response do all of you take when someone screws up like this? How do you prevent it?
Also, what kind of training do you give your students prior to letting them loose on an SEM? We're about to replace our SEM with a new one with lots of features. I'm trying to get a feel for what normal training practices are and what works and doesn't work in your facility. I've had two incidents similar to this in a year (steel valve bent at 45 degrees on sputter coater), and it seems like too many. I might need some improvements in my training methods!
I heartily agree with Debby- I too have run a Core Facility for } 20 years, and was a primary user of a Core at another institution before that. I modeled my rules after the ones at that institution. They are, like Debby's few but strict: EVERYONE who wishes to use an instrument, must be trained by Facility staff. NO second-hand training is allowed for the reasons Debby mentioned. ALL problems should be reported to me immediately so that I can address them. Use of the Facility is a privilege, and privileges can be suspended if a user is repeatedly guilty of not following rules & instructions or is found to have mistreated an instrument.
I have, on occasion, informed at PI that person X in his/her lab would not be given independent access to a particular instrument because, after training, I did not have confidence that the person would be able to work independently.
I have been fortunate. In 23 years, I have experienced only minor use-generated problems with any of the instruments in my 2 facilities (EM and Optical Microscopy). I did have to send the motorized stage on my LM out for repair because users had repeatedly tried to drive it past its limit because they were not attentive to what they were doing. I was able to narrow it down to a very small handful of possible miscreants....all of whom denied doing anything wrong (of course). I told all my users how much the repair cost, and that if I had to send the part out again for the same repair, I would divide the cost evenly among the labs who used that particular microscope. This did seem to put them on notice and there have been no new signs of abuse.
Strict training (I give my confocal users a minimum of 4 hours...usually 6)and clearly written instructions go a long way to insuring long-life for your instrument. Good luck, Lee
On 02/26/11, dsherman-at-purdue.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver (http://www.microscopy.com/MicroscopyListserver) } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html (http://www.microscopy.com/MicroscopyListserver/FAQ.html) } ---------------------------------------------------------------------------- } } Robin, } } I have been teaching microscopy and managing/directing a good sized } service/multi-user EM facility for over 20 years (additional time as a user } or service provider in smaller facilities). I am very happy to say that in } that period we have had extremely few user accidents. I attribute that to } three major reasons: } 1) good training for all users before they are given their flying license. } 2) support by facility staff to assist, answer questions, continue to } train/educate on a routine basis. } 3) A certain amount of fear that users can loose access privileges if they } misuse instruments/break rules/do not respect other users. } } (1) requires offering (and requiring) courses where students learn basic } theory and hands-on use of major instrumentation....during which they are } instructed in both what to do and what not to do. If you are not willing to } put in the time required to train/educate users than you and the instruments } will run into problems at some point. It all starts from learning correctly } in the beginning with the emphasis on good habits, sufficient understanding } to make good choices, and thus get good results. Users training users is } problematic in my experience, as bad habits get passed on and magnified over } time. } } 2) A good relationship between facility staff and users results in users who } are not hesitant to ask for help. This means knowing your users, showing an } interest in them and their projects and offering assistance when asked. You } will quickly learn who needs some additional help along the way and who can } work truly independently. } } 3) I don't apologize for this one. Facility rules are there for a reason. } Down instruments due to user error affect many and that is unfair. The } primary rule I have always enforces is that users to do not attempt to fix } anything at any time. What seems simple often is a sign of much more } significant problems that can be averted if the staff knows about them and } responds accordingly. Failure to follow this rule results in much closer } scrutiny and potential loss of privileges (rarely necessary). } } All users may not perform at the same level but that does not mean that all } cannot learn to use instruments with respect for the instrument and their } fellow users. Part of our jobs as facility staff is to teach, train, and } support to insure that this happens. We may not be 100% successful, but the } number and severity of incidents will decrease significantly as a result. } } Debby } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy (http://www.ag.purdue.edu/facilities/microscopy) } } } } } On 2/26/11 11:33 AM, "microscopylistserver-noreply-at-microscopy.com" } {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver (http://www.microscopy.com/MicroscopyListserver) } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html (http://www.microscopy.com/MicroscopyListserver/FAQ.html) } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html (http://www.microscopy.com/MLFormMail.html) } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both rfoley-at-uab.edu as well as the MIcroscopy } } Listserver } } --------------------------------------------------------------------------- } } } } Email: rfoley-at-uab.edu Name: Robin D Foley } } } } Organization: UAB } } } } Title-Subject: [Filtered] Hall of Shame - Student Users } } } } Message: Question for university facility managers - } } } } Yesterday, on my very old SEM, a student user accidentally engaged the } } lock on the tilt. When the stage would not tilt, he decided to force it } } and snapped off the 5 mm diameter steel rod that you move to tilt the } } stage. He REALLY had to force it to cause this to happen. I'm sort of } } wondering if he beat it with a hammer - but I don't see any signs of } } that sort of damage! } } } } What response do all of you take when someone screws up like this? How } } do you prevent it? } } } } Also, what kind of training do you give your students prior to letting } } them loose on an SEM? We're about to replace our SEM with a new one } } with lots of features. I'm trying to get a feel for what normal } } training practices are and what works and doesn't work in your facility. } } I've had two incidents similar to this in a year (steel valve bent at } } 45 degrees on sputter coater), and it seems like too many. I might need } } some improvements in my training methods! } } } } Thanks, } } } } Robin Foley } } } } Login Host: 138.26.80.163 } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 26 10:31:55 } } 2011 } } 13, 26 -- Received: from znl.com ([206.69.208.20]) } } 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p1QGVtPO027237 } } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } } 13, 26 -- Received: from localhost (localhost [127.0.0.1]) } } 13, 26 -- by znl.com (Postfix) with ESMTP id 2C77F342902 } } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } } (CST) } } 13, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 13, 26 -- Received: from znl.com ([127.0.0.1]) } } 13, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } } 10024) } } 13, 26 -- with ESMTP id Prs6CKcsW5+r for {microscopy-at-microscopy.com} ; } } 13, 26 -- Sat, 26 Feb 2011 10:31:54 -0600 (CST) } } 13, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } } 13, 26 -- by znl.com (Postfix) with ESMTPA id AD8713428F7 } } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:54 -0600 } } (CST) } } 13, 26 -- Message-ID: {4D692AFA.3040603-at-microscopy.com} } } 13, 26 -- Date: Sat, 26 Feb 2011 10:31:54 -0600 } } 13, 26 -- From: MicroscopyListserver-NoReply } } {microscopylistserver-noreply-at-microscopy.com} } } 13, 26 -- Reply-To: rfoley-at-uab.edu } } 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 13, 26 -- MIME-Version: 1.0 } } 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 13, 26 -- Subject: [Filtered] MicroscopyListserverviaWWW:Question for } } university facility } } 13, 26 -- managers - Student Users } } 13, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 13, 26 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 15, 30 -- From dsherman-at-purdue.edu Sat Feb 26 15:31:30 2011 } 15, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) } 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1QLVTPm030583 } 15, 30 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 15:31:29 -0600 } 15, 30 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) } 15, 30 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p1QLVTT9008710 } 15, 30 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 16:31:29 -0500 } 15, 30 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB02F.purdue.lcl } 15, 30 -- ([::1]) with mapi; Sat, 26 Feb 2011 16:31:29 -0500 } 15, 30 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 15, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} , } 15, 30 -- "rfoley-at-uab.edu" } 15, 30 -- {rfoley-at-uab.edu} } 15, 30 -- Date: Sat, 26 Feb 2011 16:31:27 -0500 } 15, 30 -- Subject: Re: [Microscopy] for university facility } 15, 30 -- Thread-Topic: [Microscopy] for university facility } 15, 30 -- Thread-Index: AcvV0uH/+4/aT9+DRJOYp4UA4HB25AAKaQy/ } 15, 30 -- Message-ID: {C98EDB5F.C2C8%dsherman-at-purdue.edu} } 15, 30 -- Accept-Language: en-US } 15, 30 -- Content-Language: en-US } 15, 30 -- X-MS-Has-Attach: } 15, 30 -- X-MS-TNEF-Correlator: } 15, 30 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 15, 30 -- acceptlanguage: en-US } 15, 30 -- Content-Type: text/plain; charset="us-ascii" } 15, 30 -- MIME-Version: 1.0 } 15, 30 -- X-PMX-Version: 5.5.9.388399 } 15, 30 -- X-PerlMx-Virus-Scanned: Yes } 15, 30 -- Content-Transfer-Encoding: 8bit } 15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1QLVTPm030583 } ==============================End of - Headers============================== } } -- Lee Cohen-Gould, M.S., C.E.M.T. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Robin, I will add, only briefly, the answer to your critical question. How to prevent?
I have a relatively new ESEM which has a serious software quirk, and it can manifest it no matter who is operating. The consequence is that the stage rises at its greatest velocity until it collides with the Backscatter detector - usually deforming it . We know, after a few years, how to activate the glitch, but I will not teach THAT! Instead, I have over the years adopted the following instructional routine which has alleviated the consequence for the many undergraduates who have been trained. I am obdurate in the sequence of startup steps that establish a safety net for the BSD. All have taken to that sequence, because it makes sense, and, because I make clear the cost of deviating from it - i.e.,a new BSD at just under $10,000.00. We have had 4 or 5 replaced, but none lately.
The other thing I do during all training sessions is to tell the trainee everything I have been able to think of that can harm the instrument. I also have a yellow printout on the wall, which all have to read, so that they know that I have glitches as well and neglect to instruct ALL who do not specifically request an understanding of how to activate and use the 'stage clamp' - thus improving the possibility of acquiring high res images of 'fine' structure.
I suspect that in the case of the tilt bar, you have given us a unique - how the h.... did he do that? The cure is to make clear that as old as your SEM is, it responds to brute force as does any gentle soul. It breaks!
My sympathy for the effort that will be required to repair an old system.
Cheers,
Fred Monson http://cmirt.wcupa.edu
________________________________________ X-from: ron.doole-at-materials.ox.ac.uk [ron.doole-at-materials.ox.ac.uk] Sent: Sunday, February 27, 2011 11:54 AM To: Monson, Frederick
Dear Robin,
You have had a good set of responses and the importance of patience and practical, hands on, teaching cannot be overemphasised. One piece of advice I would give is never to bawl out your user no matter how 'stupid' they have been. They must be completely confident to come to you to explain exactly how the damage occurred, if they are scared of you they will hide the damage (if possible) or not admit what happened. You are then in a worse position of discovering the problem some time later and not knowing how it occurred.
Of course rules are important, particularly users not making modifications to operating proceedures, software or hardware without authorisation, and ultimately withdrawing the access rights of someone who blatently disregards rules must be used to protect the research programs of the other users.
Our remit as a university is education and research, everyone has to learn how to treat instrumentation properly and this will occasionally result in damage, use those occasions to educate.
Regards, Ron
Mr. Ron Doole Department of Materials Senior Instrumentation Engineer. University of Oxford. Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: 26 February 2011 16:40 To: Ron Doole
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin D Foley
Organization: UAB
Title-Subject: [Filtered] Hall of Shame - Student Users
Message: Question for university facility managers -
Yesterday, on my very old SEM, a student user accidentally engaged the lock on the tilt. When the stage would not tilt, he decided to force it and snapped off the 5 mm diameter steel rod that you move to tilt the stage. He REALLY had to force it to cause this to happen. I'm sort of wondering if he beat it with a hammer - but I don't see any signs of that sort of damage!
What response do all of you take when someone screws up like this? How do you prevent it?
Also, what kind of training do you give your students prior to letting them loose on an SEM? We're about to replace our SEM with a new one with lots of features. I'm trying to get a feel for what normal training practices are and what works and doesn't work in your facility. I've had two incidents similar to this in a year (steel valve bent at 45 degrees on sputter coater), and it seems like too many. I might need some improvements in my training methods!
Yes, I have got both the Operating and the Technical Manuals of JEOL 840/JXA 840 (including WDS). Unfortunately, I do not have it as PDF but, the printed hard copy of the manuals. Let me know if it would still be helpful.
Zia ur Rahman Johnson Space Center, Houston, TX, USA zia.rahman-1-at-NASA.GOV
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear All, anybody out there who has the Operating Instructions and the Technical Manual of the Jeol 840 as PDFs? I would be very fond of getting a copy.
Thanks, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
I am beginning to see a number of messages which are "replies to forward email". Please note that if you wish your reply to a posting to go to the entire list you must send it as a NEW message to:
microscopy-at-microscopy.com
just include a copy of the original message after your reply.
If you just use your Email program to issue a "reply" it will only go to the Sender and not to the entire list. If you truely wish to reply only to the sender this is of course your prerogative.
An even more incidious issue arises if you reply to
"microscopy-noreply-at-microscopy.com"
These replies will literally go into a black hole. This is particularly important for messages which are forwarded as "via WWW" these are postings which come in via the Web base form and will most susceptible to this problem.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America E.P. Wigner Fellow - Oak Ridge National Laboratory Senior Fellow of the Computational Institute - University of Chicago President Microscopy Soceity of America
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 18, 25 -- From zaluzec-at-aaem.amc.anl.gov Mon Feb 28 08:31:24 2011 18, 25 -- Received: from znl.com ([206.69.208.20]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1SEVOBV015234 18, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 08:31:24 -0600 18, 25 -- Received: from localhost (localhost [127.0.0.1]) 18, 25 -- by znl.com (Postfix) with ESMTP id E37A6344FB9 18, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 08:31:24 -0600 (CST) 18, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 25 -- Received: from znl.com ([127.0.0.1]) 18, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 25 -- with ESMTP id 4aUs4yz6xo2L for {microscopy-at-microscopy.com} ; 18, 25 -- Mon, 28 Feb 2011 08:31:24 -0600 (CST) 18, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 18, 25 -- by znl.com (Postfix) with ESMTPA id 72D84344FAE 18, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 08:31:24 -0600 (CST) 18, 25 -- Message-ID: {4D6BB1BC.7080107-at-aaem.amc.anl.gov} 18, 25 -- Date: Mon, 28 Feb 2011 08:31:24 -0600 18, 25 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 18, 25 -- Organization: ANL 18, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 18, 25 -- MIME-Version: 1.0 18, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 18, 25 -- Subject: Administrivia: Replying to Listserver Messages 18, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] EM facility
Message: We now have some guests from an other organization working on our TEMs, up to now without any damage or other trouble, but of course this is no garantee. We do not charge anything for noncommercial institutes unless they start to use it a lot. Does anybody out there uses contracts for EM users from outside companies/institutes to prevent discussions on costs for repair? And is there anything in that contract about costs for consumables/beam time? Could you please sent me an example (off the list)of such a contract? Thanks in advance, Hans.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pveril-at-apae.uth.gr as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hello everybody. We have a Philips CM10 transmission electron microscope and i have a strange problem. When i power up the microscope the CRT monitor seems to be dead but the microscope begins to create vacuum. The monitor worked just fine the last time that the microscope was used. It was shut down property and nobody worked with it for a very long time (about 5 months). Does anybody has any idea how can i overcome this problem? Login Host: 94.66.182.158 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 28 08:57:14 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1SEvEn7000346 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 08:57:14 -0600 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id DFB1B34506A 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 08:57:14 -0600 (CST) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id MGKlc3fnrxOm for {microscopy-at-microscopy.com} ; 7, 25 -- Mon, 28 Feb 2011 08:57:14 -0600 (CST) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id B0A4D34505F 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 08:57:14 -0600 (CST) 7, 25 -- Message-ID: {4D6BB7CA.9090601-at-microscopy.com} 7, 25 -- Date: Mon, 28 Feb 2011 08:57:14 -0600 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: pveril-at-apae.uth.gr 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:CM10 problem 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
As always, great information and advice on the subject by the listserver.
As for the repair costs, check with your business office to see if it is covered by the University's insurance. Years ago a student blew out the EDS window by not following directions and on a hunch I called our Business Office to learn that this type of accident was covered by the University of Missouri's insurance policy.
Our lab only had to pay the $1000 deductible out of the ~$10K repair. Since then we have also filed a claim to cover a broken BSE detector, similar repair costs and same deductible. This sure lessens the burden and gives the facility staff a little peace of mind for the "learn from your mistakes" part of the education process.
Good Luck, Lou -- Sr. Research Specialist University of Missouri Electron Microscopy Core Facility W136 Veterinary Medicine Building Columbia, MO 65211 573.882.4777, fax 573.884.2227 RossLM-at-missouri.edu http://www.emc.missouri.edu/
==============================Original Headers============================== 6, 28 -- From RossLM-at-missouri.edu Mon Feb 28 09:05:33 2011 6, 28 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1SF5TGQ025870 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 09:05:33 -0600 6, 28 -- X-IronPort-Anti-Spam-Filtered: true 6, 28 -- X-IronPort-Anti-Spam-Result: AvsEADBIa03Raua1/2dsb2JhbACmRnSzOYhqgxiCSQSFEA 6, 28 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) 6, 28 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 28 Feb 2011 09:05:29 -0600 6, 28 -- Received: from um-email05.um.umsystem.edu ([169.254.1.133]) by 6, 28 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Mon, 28 Feb 2011 6, 28 -- 09:05:29 -0600 6, 28 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 6, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 28 -- Date: Mon, 28 Feb 2011 09:05:26 -0600 6, 28 -- Subject: Question for..... 6, 28 -- Thread-Topic: Question for..... 6, 28 -- Thread-Index: AcvXWO37FWsu1thmH06vjbKiP9UFHw== 6, 28 -- Message-ID: {C99115D6.1A886%rosslm-at-missouri.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1SF5TGQ025870 ==============================End of - Headers==============================
Our CM10 had its CRT replaced last year, as it was taking about 20 minutes to warm up enough to be visible (from start up of the machine). Just to check the obvious: the data dim knob is turned fully to the right? The panel dim knob is pushed in? And the light sensor is uncovered and getting enough light? If these things are OK, I would guess the CRT has just died.
You may be able to find a used (but still good) one (as we did) if you have a friendly EM engineer.
Good luck,
Ben
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271866 {http://mrcanu.pharm.ox.ac.uk/}
} } Email: pveril-at-apae.uth.gr Name: Berillis Panagiotis } } Organization: University of Thessaly } } Title-Subject: [Filtered] CM10 problem } } Message: Hello everybody. We have a Philips CM10 transmission electron } microscope and i have a strange problem. When i power up the microscope } the CRT monitor seems to be dead but the microscope begins to create } vacuum. The monitor worked just fine the last time that the microscope } was used. It was shut down property and nobody worked with it for a very } long time (about 5 months). Does anybody has any idea how can i overcome } this problem? } Login Host: 94.66.182.158 } --------------------------------------------------------------------------
==============================Original Headers============================== 12, 30 -- From ben.micklem-at-pharm.ox.ac.uk Mon Feb 28 09:59:32 2011 12, 30 -- Received: from relay5.mail.ox.ac.uk (relay5.mail.ox.ac.uk [163.1.2.163]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1SFxWXl016297 12, 30 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 09:59:32 -0600 12, 30 -- Received: from smtp3.nexus.ox.ac.uk ([163.1.154.137] helo=exht03.ad.oak.ox.ac.uk) 12, 30 -- by relay5.mail.ox.ac.uk with esmtp (Exim 4.74) 12, 30 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 12, 30 -- id 1Pu5VT-0003ns-IY 12, 30 -- for microscopy-at-microscopy.com; Mon, 28 Feb 2011 15:59:31 +0000 12, 30 -- Received: from EXMBX04.ad.oak.ox.ac.uk ([169.254.2.68]) by 12, 30 -- exht03.ad.oak.ox.ac.uk ([163.1.154.54]) with mapi; Mon, 28 Feb 2011 15:59:31 12, 30 -- +0000 12, 30 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 12, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 30 -- Date: Mon, 28 Feb 2011 15:59:30 +0000 12, 30 -- Subject: [Microscopy] viaWWW:CM10 problem 12, 30 -- Thread-Topic: [Microscopy] viaWWW:CM10 problem 12, 30 -- Thread-Index: AcvXYHylzj3t20X4SoykVECTI1Tqig== 12, 30 -- Message-ID: {C9917687.F671%ben.micklem-at-pharm.ox.ac.uk} 12, 30 -- In-Reply-To: {C9917328.F656%ben.micklem-at-pharm.ox.ac.uk} 12, 30 -- Accept-Language: en-US, en-GB 12, 30 -- Content-Language: en-US 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- user-agent: Microsoft-MacOutlook/14.2.0.101115 12, 30 -- acceptlanguage: en-US, en-GB 12, 30 -- Content-Type: text/plain; charset="us-ascii" 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1SFxWXl016297 ==============================End of - Headers==============================
Dear Robin, Here's my 2 cents in addition to all the excellent suggestions you've gotten so far. When I was in charge of facilities, in one case, users were assigned to several categories. For the lowest category users, the staff did everything except say which area to image, and the users were allowed to do sequentially more so that the user in the highest category could work completely on his own. The staff did all the training, and the extent of the training was geared to how much time the user was planning to spend--it was a regional resource facility, so we had users who visited only once and others who spent many days at our scope. The other case was an academic situation, probably more like yours, where each user was a student or postdoc, so each user was thoroughly trained. In that case, the user was shown each procedure until both he and the staff member were confident that he knew how to do the procedure, then the staff member watched the user do the procedure until, again, both were confident, and finally, the user was allowed to do the procedure on his own. In all cases, the users were firmly instructed to come and get me (or call my cell phone or pager if I could not immediately be found) and let me know if anything unexpected happened. This instruction was to be followed even if it was 3 AM--I kept my pager on when I knew a user was to be on the scope. This worked pretty well except for one user who did not accept authority. His culture did not accord women equal status, and since his mentor was a woman, he had a tendency not to listen to her. Eventually, I was able to get him to realize that I had the bona fides for him to listen to me, and things got better, but not before he had introduced a virus into the software and made other problems for everyone. It is impossible to idiot-proof everything, since there is a never-ending supply of new idiots, but one can keep it as a goal. I hope you can use some of this in your situation. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin D Foley
Organization: UAB
Title-Subject: [Filtered] Hall of Shame - Student Users
Message: Question for university facility managers -
Yesterday, on my very old SEM, a student user accidentally engaged the lock on the tilt. When the stage would not tilt, he decided to force it and snapped off the 5 mm diameter steel rod that you move to tilt the stage. He REALLY had to force it to cause this to happen. I'm sort of wondering if he beat it with a hammer - but I don't see any signs of that sort of damage!
What response do all of you take when someone screws up like this? How do you prevent it?
Also, what kind of training do you give your students prior to letting them loose on an SEM? We're about to replace our SEM with a new one with lots of features. I'm trying to get a feel for what normal training practices are and what works and doesn't work in your facility. I've had two incidents similar to this in a year (steel valve bent at 45 degrees on sputter coater), and it seems like too many. I might need some improvements in my training methods!
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1SL8WTN026388 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 28 Feb 2011 15:08:32 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p1SL8WWG026386; Mon, 28 Feb 2011 15:08:32 -0600
Excellent suggestions from the previous responses.
I've been involved in centralized facilities for over 30 years and have experienced the good, the marginal and the really bad.
There is no substitute for thorough training and testing prior to turning users loose with equipment. We offer both formal (for credit) graduate level courses in SEM and TEM as well as individualized training for researchers with various levels of experience.
After the training (that covers theory and hands-on use of the equipment), all users submit to a "check out" examination. This consists of 10 or so written, basic questions about the instrument (precautions mostly) and then the researcher is asked to demonstrate their competency (insert specimen, use the instrument under various conditions, record images, remove specimen). Most people do pass the exam, but we have had several individuals who could only use the instrument with assistance from our staff.
We are extremely careful with our instruments since we have little to no funding to replace damaged components. Even our oldest instrument, a 28 yr old Hitachi S570, still meets original specifications and is quite clean in appearance. Everyone knows that controls are to be adjusted to "microscope tight" specifications. That is, do not over tighten and call someone if something is stuck.
If researchers are really interested in learning to use the instruments, they will learn how to use them correctly. The problem cases almost always involve someone who is only marginally interested in the instrument and regards it as a basic tool to achieve their goals. They are often in a hurry to obtain data for an overdue report and don't want to waste time finessing the instrument. We all know the result: poor images, sloppy work area and sometimes damage to the instrument.
One concept that does seem to work: "time is money." If you do not spend the time to learn the instrument, you are wasting money since the imaging will have to be repeated (the paper or dissertation will be rejected, the grant not funded, etc.). When it costs them money, people listen.
Even with all of this, accidents still do happen since we are imperfect beings.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 10, 20 -- From bozzola-at-siu.edu Mon Feb 28 15:08:32 2011 10, 20 -- Received: from mail-qy0-f169.google.com (mail-qy0-f169.google.com [209.85.216.169]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p1SL8WR8026383 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 15:08:32 -0600 10, 20 -- Received: by qyk2 with SMTP id 2so2750986qyk.0 10, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 13:08:31 -0800 (PST) 10, 20 -- MIME-Version: 1.0 10, 20 -- Received: by 10.224.3.4 with SMTP id 4mr5177657qal.109.1298927311741; Mon, 28 10, 20 -- Feb 2011 13:08:31 -0800 (PST) 10, 20 -- Received: by 10.229.220.145 with HTTP; Mon, 28 Feb 2011 13:08:31 -0800 (PST) 10, 20 -- In-Reply-To: {201102282043.p1SKh3HU010863-at-ns.microscopy.com} 10, 20 -- References: {201102282043.p1SKh3HU010863-at-ns.microscopy.com} 10, 20 -- Date: Mon, 28 Feb 2011 15:08:31 -0600 10, 20 -- Message-ID: {AANLkTimimiVhnkrRUE4d+TXwjfRRU_DE+nFPng9g720f-at-mail.gmail.com} 10, 20 -- Subject: Re: [Microscopy] Re: [Filtered] MicroscopyListserverviaWWW:Question for 10, 20 -- From: John Bozzola {bozzola-at-siu.edu} 10, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 10, 20 -- Content-Type: text/plain; charset=UTF-8 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p1SL8WR8026383 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] CM10 problem
Message: This seems to be a defective monitor, because the program started up okay, so the self test of the internal computer was performed well. Maybe there is just a loose connector. Good luck, Hans.
Message: Hello everybody. We have a Philips CM10 transmission electron microscope and i have a strange problem. When i power up the microscope the CRT monitor seems to be dead but the microscope begins to create vacuum. The monitor worked just fine the last time that the microscope was used. It was shut down property and nobody worked with it for a very long time (about 5 months). Does anybody has any idea how can i overcome this problem? Login Host: 94.66.182.158
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbarlow-at-sciences.sdsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] OCF scheduler problem
Message: Dear listers
We have been using the OCF Scheduler software developed at UofA. Upon upgrading our PHP server from version 5.2 to 5.3, the software no longer works properly. Has anyone else discovered the same thing? Anyone else got a fix? There have been rumors funding was being sought to update/upgrade this program, but so far as I know, these plans, and an updated version, remain pie in the sky.
I really liked the potential in this software, particularly the ability to record log on and log off of an instrument running on a windows box to a central database.
Given the repeated requests for scheduling software on the listserve, might this be something the Facility manager FIG, in conjunction with MSA, might be able to develop in conjunction with the instrument manufacturers and NSF/NIH? After all, many grant applications and final reports ask for user hours information.
I too agree with Debby and Lee. I would add that during the training you can usually "feel" how your to-be-user is functionning and what is his risk management strategy. Does he listen carefully at your instructions? Does he hesitate before performing an action (which is actually a good sign: he thinks before doing)? I would add something else: my strategy is to give the impression that the instrument is extremely complex and delicate (which is not far from the truth) and also that access to this sort of instrument is an extraordinary privilege. The user should not take his work easy. Thinking is not an option, it is an obligation! During the training I deliberately emphasize that the user must follow very precise instructions. I think that the worst would be to say "well just sit down and try it yourself, it is the best way to learn". In brief: I do my best to impress and I deliberately show a strict and severe profile. I am not cool at all at the electron microscope! I think this gives the user a sense of responsability for this instrument and they are more careful with it.
Stephane
----- Original Message ---- X-from: "lcgould-at-med.cornell.edu" {lcgould-at-med.cornell.edu} To: nizets2-at-yahoo.com Sent: Sun, February 27, 2011 9:14:31 PM
I heartily agree with Debby- I too have run a Core Facility for } 20 years, and was a primary user of a Core at another institution before that. I modeled my rules after the ones at that institution. They are, like Debby's few but strict: EVERYONE who wishes to use an instrument, must be trained by Facility staff. NO second-hand training is allowed for the reasons Debby mentioned. ALL problems should be reported to me immediately so that I can address them. Use of the Facility is a privilege, and privileges can be suspended if a user is repeatedly guilty of not following rules & instructions or is found to have mistreated an instrument.
I have, on occasion, informed at PI that person X in his/her lab would not be given independent access to a particular instrument because, after training, I did not have confidence that the person would be able to work independently.
I have been fortunate. In 23 years, I have experienced only minor use-generated problems with any of the instruments in my 2 facilities (EM and Optical Microscopy). I did have to send the motorized stage on my LM out for repair because users had repeatedly tried to drive it past its limit because they were not attentive to what they were doing. I was able to narrow it down to a very small handful of possible miscreants....all of whom denied doing anything wrong (of course). I told all my users how much the repair cost, and that if I had to send the part out again for the same repair, I would divide the cost evenly among the labs who used that particular microscope. This did seem to put them on notice and there have been no new signs of abuse.
Strict training (I give my confocal users a minimum of 4 hours...usually 6)and clearly written instructions go a long way to insuring long-life for your instrument. Good luck, Lee
On 02/26/11, dsherman-at-purdue.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } (http://www.microscopy.com/MicroscopyListserver) } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } (http://www.microscopy.com/MicroscopyListserver/FAQ.html) } ---------------------------------------------------------------------------- } } Robin, } } I have been teaching microscopy and managing/directing a good sized } service/multi-user EM facility for over 20 years (additional time as a user } or service provider in smaller facilities). I am very happy to say that in } that period we have had extremely few user accidents. I attribute that to } three major reasons: } 1) good training for all users before they are given their flying license. } 2) support by facility staff to assist, answer questions, continue to } train/educate on a routine basis. } 3) A certain amount of fear that users can loose access privileges if they } misuse instruments/break rules/do not respect other users. } } (1) requires offering (and requiring) courses where students learn basic } theory and hands-on use of major instrumentation....during which they are } instructed in both what to do and what not to do. If you are not willing to } put in the time required to train/educate users than you and the instruments } will run into problems at some point. It all starts from learning correctly } in the beginning with the emphasis on good habits, sufficient understanding } to make good choices, and thus get good results. Users training users is } problematic in my experience, as bad habits get passed on and magnified over } time. } } 2) A good relationship between facility staff and users results in users who } are not hesitant to ask for help. This means knowing your users, showing an } interest in them and their projects and offering assistance when asked. You } will quickly learn who needs some additional help along the way and who can } work truly independently. } } 3) I don't apologize for this one. Facility rules are there for a reason. } Down instruments due to user error affect many and that is unfair. The } primary rule I have always enforces is that users to do not attempt to fix } anything at any time. What seems simple often is a sign of much more } significant problems that can be averted if the staff knows about them and } responds accordingly. Failure to follow this rule results in much closer } scrutiny and potential loss of privileges (rarely necessary). } } All users may not perform at the same level but that does not mean that all } cannot learn to use instruments with respect for the instrument and their } fellow users. Part of our jobs as facility staff is to teach, train, and } support to insure that this happens. We may not be 100% successful, but the } number and severity of incidents will decrease significantly as a result. } } Debby } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy } (http://www.ag.purdue.edu/facilities/microscopy) } } } } } On 2/26/11 11:33 AM, "microscopylistserver-noreply-at-microscopy.com" } {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } (http://www.microscopy.com/MicroscopyListserver) } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } (http://www.microscopy.com/MicroscopyListserver/FAQ.html) } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } (http://www.microscopy.com/MLFormMail.html) } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both rfoley-at-uab.edu as well as the MIcroscopy } } Listserver } } --------------------------------------------------------------------------- } } } } Email: rfoley-at-uab.edu Name: Robin D Foley } } } } Organization: UAB } } } } Title-Subject: [Filtered] Hall of Shame - Student Users } } } } Message: Question for university facility managers - } } } } Yesterday, on my very old SEM, a student user accidentally engaged the } } lock on the tilt. When the stage would not tilt, he decided to force it } } and snapped off the 5 mm diameter steel rod that you move to tilt the } } stage. He REALLY had to force it to cause this to happen. I'm sort of } } wondering if he beat it with a hammer - but I don't see any signs of } } that sort of damage! } } } } What response do all of you take when someone screws up like this? How } } do you prevent it? } } } } Also, what kind of training do you give your students prior to letting } } them loose on an SEM? We're about to replace our SEM with a new one } } with lots of features. I'm trying to get a feel for what normal } } training practices are and what works and doesn't work in your facility. } } I've had two incidents similar to this in a year (steel valve bent at } } 45 degrees on sputter coater), and it seems like too many. I might need } } some improvements in my training methods! } } } } Thanks, } } } } Robin Foley } } } } Login Host: 138.26.80.163 } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 13, 26 -- From microscopylistserver-noreply-at-microscopy.com Sat Feb 26 } 10:31:55 } } 2011 } } 13, 26 -- Received: from znl.com ([206.69.208.20]) } } 13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p1QGVtPO027237 } } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } } 13, 26 -- Received: from localhost (localhost [127.0.0.1]) } } 13, 26 -- by znl.com (Postfix) with ESMTP id 2C77F342902 } } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:55 -0600 } } (CST) } } 13, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 13, 26 -- Received: from znl.com ([127.0.0.1]) } } 13, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port } } 10024) } } 13, 26 -- with ESMTP id Prs6CKcsW5+r for {microscopy-at-microscopy.com} ; } } 13, 26 -- Sat, 26 Feb 2011 10:31:54 -0600 (CST) } } 13, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } } 13, 26 -- by znl.com (Postfix) with ESMTPA id AD8713428F7 } } 13, 26 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 10:31:54 -0600 } } (CST) } } 13, 26 -- Message-ID: {4D692AFA.3040603-at-microscopy.com} } } 13, 26 -- Date: Sat, 26 Feb 2011 10:31:54 -0600 } } 13, 26 -- From: MicroscopyListserver-NoReply } } {microscopylistserver-noreply-at-microscopy.com} } } 13, 26 -- Reply-To: rfoley-at-uab.edu } } 13, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 13, 26 -- MIME-Version: 1.0 } } 13, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 13, 26 -- Subject: [Filtered] MicroscopyListserverviaWWW:Question for } } university facility } } 13, 26 -- managers - Student Users } } 13, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 13, 26 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 15, 30 -- From dsherman-at-purdue.edu Sat Feb 26 15:31:30 2011 } 15, 30 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu } [128.210.5.129]) } 15, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p1QLVTPm030583 } 15, 30 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 15:31:29 -0600 } 15, 30 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu } [172.21.6.91]) } 15, 30 -- by mailhub129.itcs.purdue.edu } (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p1QLVTT9008710 } 15, 30 -- for {microscopy-at-microscopy.com} ; Sat, 26 Feb 2011 16:31:29 -0500 } 15, 30 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by } WPPEXHUB02F.purdue.lcl } 15, 30 -- ([::1]) with mapi; Sat, 26 Feb 2011 16:31:29 -0500 } 15, 30 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 15, 30 -- To: "message to: MSA list" {microscopy-at-microscopy.com} , } 15, 30 -- "rfoley-at-uab.edu" } 15, 30 -- {rfoley-at-uab.edu} } 15, 30 -- Date: Sat, 26 Feb 2011 16:31:27 -0500 } 15, 30 -- Subject: Re: [Microscopy] for university facility } 15, 30 -- Thread-Topic: [Microscopy] for university facility } 15, 30 -- Thread-Index: AcvV0uH/+4/aT9+DRJOYp4UA4HB25AAKaQy/ } 15, 30 -- Message-ID: {C98EDB5F.C2C8%dsherman-at-purdue.edu} } 15, 30 -- Accept-Language: en-US } 15, 30 -- Content-Language: en-US } 15, 30 -- X-MS-Has-Attach: } 15, 30 -- X-MS-TNEF-Correlator: } 15, 30 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 15, 30 -- acceptlanguage: en-US } 15, 30 -- Content-Type: text/plain; charset="us-ascii" } 15, 30 -- MIME-Version: 1.0 } 15, 30 -- X-PMX-Version: 5.5.9.388399 } 15, 30 -- X-PerlMx-Virus-Scanned: Yes } 15, 30 -- Content-Transfer-Encoding: 8bit } 15, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p1QLVTPm030583 } ==============================End of - Headers============================== } } -- Lee Cohen-Gould, M.S., C.E.M.T. Sr. Staff Associate in Biochemistry and Cell & Developmental Biology Director, Electron Microscopy & Histology and Optical Microscopy Core Facilities Weill Cornell Medical College
Here is the response I received from my IT specialist:
We intentionally do no not upgrade that server because of these potential issues. It's like one of those old Bell telephones, not fancy but it keeps going and doesn't break.
Aaron
-------- Original Message --------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbarlow-at-sciences.sdsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] OCF scheduler problem
Message: Dear listers
We have been using the OCF Scheduler software developed at UofA. Upon upgrading our PHP server from version 5.2 to 5.3, the software no longer works properly. Has anyone else discovered the same thing? Anyone else got a fix? There have been rumors funding was being sought to update/upgrade this program, but so far as I know, these plans, and an updated version, remain pie in the sky.
I really liked the potential in this software, particularly the ability to record log on and log off of an instrument running on a windows box to a central database.
Given the repeated requests for scheduling software on the listserve, might this be something the Facility manager FIG, in conjunction with MSA, might be able to develop in conjunction with the instrument manufacturers and NSF/NIH? After all, many grant applications and final reports ask for user hours information.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Feb 28 20:01:39 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2121dtQ032051 12, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 20:01:39 -0600 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id 25B9C345A00 12, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 20:01:39 -0600 (CST) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id PIIh3Q0OTRtu for {microscopy-at-microscopy.com} ; 12, 25 -- Mon, 28 Feb 2011 20:01:36 -0600 (CST) 12, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 12, 25 -- by znl.com (Postfix) with ESMTPA id 6557B3459F5 12, 25 -- for {microscopy-at-microscopy.com} ; Mon, 28 Feb 2011 20:01:36 -0600 (CST) 12, 25 -- Message-ID: {4D6C5380.8010205-at-microscopy.com} 12, 25 -- Date: Mon, 28 Feb 2011 20:01:36 -0600 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: sbarlow-at-sciences.sdsu.edu 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 --
EM'ers: I have enjoyed this thread and have followed it pretty closely. I guess human nature is human nature because much of what I have read I also could have written since I have seen the same instances. One of the ways I have identified those students who may be trouble is if they take notes or not when I am showing them the instrument. Those who fail to take notes usually are the worst users. I tell all of my students to take notes because their notes will be their user's manual. I feel that if they have to write things down at least they are processing the material at least once. On the first session with one of my recent students I told him to take notes and I saw that he wasn't. I then showed him four times how to put in and take out the sample holder. On his first attempt to put in the holder he did exactly what I told him not to do and he blew the vacuum. On his next session with me I told him three times to take notes and he finally did. I am obviously keeping my eye on him.
I have heard a number of you say that it is mandatory at your facility for facility staff to do the training. I fully agree with that but here I don't have that option. I have two assistant faculty members who, unfortunately, are on the faculty advisory committee. They decided that they were going to train their own people. We have already seen problems because of that. At the very least I have had to go behind them and fill in where their training has been lacking. Just yesterday a student of one of the faculty messed up my carbon evaporator enough that I had to take it apart and clean everything and that wasn't the first time this student messed up. Luckily, those two faculty members are not heavy users.
Norm Olson
} From: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } To: "Olson, Norman" {nholson-at-ucsd.edu} } Date: Tue, 1 Mar 2011 01:00:29 -0800 } Subject: [Microscopy] Re: for university facility } Thread-Topic: [Microscopy] Re: for university facility } Thread-Index: AcvX7x428ognZnidQ1SPsscve/dsWQ== } Reply-To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} } Accept-Language: en-US } x-ironport-av: E=Sophos;i="4.62,246,1297065600"; } d="scan'208";a="447626855" } x-resent-from: "Microscopy Listserver" {microscopy-at-microscopy.com} } x-liewp: MicroscopyListSpam NAGS } x-ironport-delayed-alias: True } x-ironport-anti-spam-slbl-result: SAFE-LISTED } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
You may want to consider an alternative scheduling system FOM (Facility Online Manager at http://www.FOMNetworks.com/ ). Numerous EM facilities have decided to use FOM system.
The limited version costs only $100 for life time use supporting unlimited number of instruments and users in one facility.
The limited version of FOM provides scheduling function similar to Calcium or OCF system, plus inventory management, collaboration tracking (upload/download exp results and pdf files), survey function and email lists. The full version of FOM also supports hardware access control to the instruments, billing and reporting. We also customize the system for you if needed.
To see a comparison of popular instrument scheduling systems used, please visit http://www.fomnetworks.com/Compare_online_scheduling_software.html
As the founder of FOM Networks, I am open to discuss any possibilities with Facility Manager FIG and/or MSA in order for more EM facilities to benefit from the well-established Facility Online Manager system.
DISCLAIMER: This email is sent on behalf of FOM Networks, Inc.
Best Regards, Shuyou Li _________________ Shuyou Li, Ph.D. FOM Networks, Inc. www.fomnetworks.com 224-225-9168
-------- Original Message --------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sbarlow-at-sciences.sdsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sbarlow-at-sciences.sdsu.edu Name: Steve Barlow
Organization: SDSU EM Facility
Title-Subject: [Filtered] OCF scheduler problem
Message: Dear listers
We have been using the OCF Scheduler software developed at UofA. Upon upgrading our PHP server from version 5.2 to 5.3, the software no longer works properly. Has anyone else discovered the same thing? Anyone else got a fix? There have been rumors funding was being sought to update/upgrade this program, but so far as I know, these plans, and an updated version, remain pie in the sky.
I really liked the potential in this software, particularly the ability to record log on and log off of an instrument running on a windows box to a central database.
Given the repeated requests for scheduling software on the listserve, might this be something the Facility manager FIG, in conjunction with MSA, might be able to develop in conjunction with the instrument manufacturers and NSF/NIH? After all, many grant applications and final reports ask for user hours information.
The Australian Centre for Microscopy and Microanalysis (ACMM) at the University of Sydney, is the largest facility of its kind in Australia. We have 7 TEMs, 6 SEMs, 2 atom probes and lots of sophisticated light and optical and x-ray equipment. This brings in over 400 registered users annually, approximately 1/3rd of whom are new users. Effective training is essential to ensure users get up to speed quickly, and also to ensure that costly mistakes don't happen. The training regime we use is as follows:
1. New users attend a meeting with relevant academic/technical staff to determine what they are trying to do, how best to do it, and on which instrument.
2. Training (for TEM) is usually 3 x 3hr sessions either with a technical staff member or with one of our competent grad students. Users are taught from a standardised manual, which shows step by step procedures with lots of pictures. Users are encouraged to annotate their own copy of the manual.
3. After training users are given 1-3 supervised solo sessions, where tech staff get them started and drop in frequently to assist with any queries.
4. Once the user is confident running the machine solo they are assessed. This is two part process. The first part is a 40 question multiple choice quiz, covering basic theory, instrument operation, safety etc which is covered during training. Nobody fails the quiz - we use it as a safety net to fill in gaps in users' knowledge. The second part is an assessment, where the user goes through setting up, operating and shutting down the instrument - it's open book so they can refer to the manual. This is supervised by technical staff to ensure quality control. Users need to be able to do all the basics without undue hesitation and without harming (or nearly harming) the microscope.
5. Users who fail the test are given more training. In some cases lots more, until they get it right.
6. Users on instruments can always get immediate help from one of our technical staff by calling our Duty Microscopist number. This avoids having users wander corridors in search of staff, and avoids them getting bored and trying to fix things themselves - which is usually where things go bad.
Despite all the above, trained users stuff things up occasionally, usually through carelessness. Such users are retrained in whatever they stuffed up and watched carefully thereafter. We avoid tearing strips off users who do bad things as it discourages them from asking for help or owning up to mistakes. We strongly encourage 'if in doubt - ask'.
Regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, TEM Manager
Address: Australian Centre for Microscopy & Microanalysis Incorporating: Australian Microscopy & Microanalysis Research Facility ARC Centre of Excellence for Design in Light Metals Madsen Building F09, Room 128A The University of Sydney NSW, 2006, Australia sydney.edu.au/acmm {http://www.sydney.edu.au/acmm} www.ammrf.org.au {http://www.ammrf.org.au/}
www.dmscripting.com
==============================Original Headers============================== 19, 27 -- From drg.mitchell-at-sydney.edu.au Tue Mar 1 17:50:53 2011 19, 27 -- Received: from baghdad.ucc.usyd.edu.au (baghdad.ucc.usyd.edu.au [129.78.64.146]) 19, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p21NoqEX017166 19, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 1 Mar 2011 17:50:53 -0600 19, 27 -- Received: from baghdad.ucc.usyd.edu.au (localhost [127.0.0.1]) 19, 27 -- by localhost (Postfix) with SMTP id 2363810395E 19, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 10:50:29 +1100 (EST) 19, 27 -- Received: from ex-bri-pro-02.mcs.usyd.edu.au (ex-bri-pro-02.mcs.usyd.edu.au [172.17.63.242]) 19, 27 -- by baghdad.ucc.usyd.edu.au (Postfix) with ESMTP id 8DB66103975 19, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 10:50:28 +1100 (EST) 19, 27 -- Received: from EXPRSV01.mcs.usyd.edu.au ([172.17.63.1]) by ex-bri-pro-02.mcs.usyd.edu.au with Microsoft SMTPSVC(6.0.3790.4675); 19, 27 -- Wed, 2 Mar 2011 10:50:28 +1100 19, 27 -- Received: from 172.17.185.136 ([172.17.185.136]) by EXPRSV01.mcs.usyd.edu.au ([172.17.63.4]) via Exchange Front-End Server www.owa.usyd.edu.au ([172.17.185.6]) with Microsoft Exchange Server HTTP-DAV ; 19, 27 -- Tue, 1 Mar 2011 23:50:27 +0000 19, 27 -- User-Agent: Microsoft-Entourage/11.4.0.080122 19, 27 -- Date: Wed, 02 Mar 2011 10:50:26 +1100 19, 27 -- Subject: TEM: User Training 19, 27 -- From: David Mitchell {drg.mitchell-at-sydney.edu.au} 19, 27 -- To: {Microscopy-at-microscopy.com} 19, 27 -- Message-ID: {C993D172.B8B4%drg.mitchell-at-sydney.edu.au} 19, 27 -- Thread-Topic: TEM: User Training 19, 27 -- Thread-Index: AcvYa2/croUlVEReEeCnnAAjMrpsqg== 19, 27 -- Mime-version: 1.0 19, 27 -- Content-type: text/plain; 19, 27 -- charset="US-ASCII" 19, 27 -- Content-transfer-encoding: 7bit 19, 27 -- X-OriginalArrivalTime: 01 Mar 2011 23:50:28.0530 (UTC) FILETIME=[715E5920:01CBD86B] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfklie-at-uic.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfklie-at-uic.edu Name: Robert Klie
Organization: University of Illinois at Chicago
Title-Subject: [Filtered] Job Opening: Research Assistant Professor Position in Aberration-Corrected Scanning Transmission Electron Microscopy
Message: An Research Assistant Professor position is available in the Nanoscale Physics Group lead by Professor Robert F. Klie at the University of Illinois at Chicago to work on the application of atomic-resolution Z-contrast and annular bright-field imaging, as well as electron energy-loss spectroscopy (EELS) to functional nano-materials. The successful candidate will working with the groupÂ’s new aberration-corrected cold-field emission STEM (the JEOL ARM200-CF) with sub-Ã… and sub-eV resolution, equipped with several in-situ holders. Candidates should have a Ph.D. in Physics, Materials Science or a related discipline. The position requires extensive experience in transmission electron microscopy and electron energy-loss spectroscopy. Experience working with aberration-corrected scanning transmission electron microscopy is also preferred. This position is available starting July 1, 2011, and remains open until it is filled. For fullest consideration apply by May 15, 2011. The salary will range between $55,000 and $75,000 depending on the level of experience and seniority. Interested candidates can apply online at http://jobs.uic.edu. Please submit a cover letter, CV with a complete list of publications, and contact information of three professional references. Questions about the position should be directed to the search committee chair (Prof. Robert Klie; rfklie-at-uic.edu). UIC is an AA/EOE.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] electron microscopy and pigments
Message: Hi,
I have a researcher interested in identifying pigments from a sample. I cannot think of a way we can use TEM or SEM to identify these pigments. I think that all the pigments within the sample will have similar composition of elements and I don't think we will be able to distinguish them. Has anyone ever looked at identifying pigments from their specimens maybe a method we could try?
Thanks for your message. It seems to have elicited a large range of responses, so you have tapped a very rich seam of opinion.
We are fortunate to have quite a lot of equipment (7 TEMs, 5 SEMs and a FIB/SEM) run by a few technical staff. However, I train a lot of new TEM users so my experience is restricted to those...
Occasionally, we have problems like you've described. One student left a TEM specimen holder with a 30 degree bend in the middle of it after taking out. The culprit never admitted it (it is usually a man), but it wasn't difficult to eliminate all but him. We have also found there are cultural variations in attitudes to the equipment and this usually correlates with the degree of note-taking (this is a very early indicator for us). For example, I'm training one young lady who takes copious notes and treats the machine well; I've had others who take few or no notes and then take an extremely long time to train properly.
One useful strategy I've found when training pairs, is that I will talk one student through (they do all the work) and then, when they have completed microscope alignment, get them to talk the next guy/girl through. I've found this useful because I take a lot of microscope-jargon for granted, whereas the students often rephrase the terms to make more sense (between themselves). I interject only if they proceed down blind alleys or if the machine is in some danger.
TEM training usually takes about 15-20 hours all in all. After about 6 hours the students start to become confident to handle the machine, but the bad habits start creeping in by hour ten. Fifteen is usually the minimum to get a newbie to taking good stigmatism-free images, in focus and an ability to use the machine profitably without too many problems. We also have a driving test in which we observe the user, but try not to interfere if we can. They are usually asked several questions related to safety too vis-a-vis X-rays, liquid nitrogen handling and microscope covers/shielding.
I hope this helps.
Best, Jon Barnard
Dept of Materials Science & Metallurgy, University of Cambridge, UK.
==============================Original Headers============================== 9, 28 -- From jsb43-at-hermes.cam.ac.uk Wed Mar 2 03:10:05 2011 9, 28 -- Received: from ppsw-41.csi.cam.ac.uk (ppsw-41.csi.cam.ac.uk [131.111.8.141]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p229A4wU012165 9, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 03:10:05 -0600 9, 28 -- X-Cam-AntiVirus: no malware found 9, 28 -- X-Cam-SpamDetails: not scanned 9, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 9, 28 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:35341) 9, 28 -- by ppsw-41.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25) 9, 28 -- with esmtpa (EXTERNAL:jsb43) id 1Pui4K-0006wZ-Q0 (Exim 4.72) 9, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Wed, 02 Mar 2011 09:10:04 +0000 9, 28 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 9, 28 -- with local (PRAYER:jsb43) id 1Pui4K-00079k-1b (Exim 4.67) 9, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Wed, 02 Mar 2011 09:10:04 +0000 9, 28 -- Received: from [128.232.229.149] by webmail.hermes.cam.ac.uk 9, 28 -- with HTTP (Prayer-1.3.3); 02 Mar 2011 09:10:03 +0000 9, 28 -- Date: 02 Mar 2011 09:10:03 +0000 9, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 9, 28 -- To: rfoley-at-uab.edu 9, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 9, 28 -- Subject: Re: Question for university facility 9, 28 -- Message-ID: {Prayer.1.3.3.1103020910030.12227-at-hermes-1.csi.cam.ac.uk} 9, 28 -- In-Reply-To: {201102261637.p1QGbWXL004542-at-ns.microscopy.com} 9, 28 -- References: {201102261637.p1QGbWXL004542-at-ns.microscopy.com} 9, 28 -- X-Mailer: Prayer v1.3.3 9, 28 -- Mime-Version: 1.0 9, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 9, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
A few people have mentioned note-taking as an important indicator of whether people are training well. I have found that note-taking does not correlate with subsequent competence. However, providing a step-by-step checklist of how to turn on the instrument, set up specific imaging conditions, etc. is invaluable. This provides quality control from the most instrument phobic to the most expert. People used to say, "I can't get the machine to work." I would respond, "Did you use the checklist?" and then model the behavior of going through it step-by-step (of course, I never needed the checklist myself). -Michael Cammer
-----Original Message----- X-from: jsb43-at-cam.ac.uk [mailto:jsb43-at-cam.ac.uk] Sent: Wednesday, March 02, 2011 4:20 AM To: Cammer, Michael
I agree with the checklist concept and the importance of taking notes. In our case it is a written handout for each instrument. These are time-consuming to write initially but pay big dividends down the road. Once the students know the names/function of the knobs or have an idea of the software they should be able to follow the handouts to do basic alignment and operation. Students can then personalize as necessary for their understanding but the basics are there so the amount of required note taking is reduced. The instructions are there for quick review for those who have been off the instruments for a time and need a refresher.
Of course students have to remember to bring them with them when they come....... ( keep a copy available just in case).
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "Michael.Cammer-at-med.nyu.edu" {Michael.Cammer-at-med.nyu.edu} } Reply-To: "Michael.Cammer-at-med.nyu.edu" {Michael.Cammer-at-med.nyu.edu} } Date: Wed, 2 Mar 2011 08:23:29 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Question for university facility } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } A few people have mentioned note-taking as an important indicator of whether } people are training well. I have found that note-taking does not correlate } with subsequent competence. However, providing a step-by-step checklist of } how to turn on the instrument, set up specific imaging conditions, etc. is } invaluable. This provides quality control from the most instrument phobic to } the most expert. People used to say, "I can't get the machine to work." I } would respond, "Did you use the checklist?" and then model the behavior of } going through it step-by-step (of course, I never needed the checklist } myself). } -Michael Cammer } } -----Original Message----- } X-from: jsb43-at-cam.ac.uk [mailto:jsb43-at-cam.ac.uk] } Sent: Wednesday, March 02, 2011 4:20 AM } To: Cammer, Michael } Subject: [Microscopy] Re: Question for university facility } } and this usually correlates with } the degree of note-taking (this is a very early indicator for us). For } example, I'm training one young lady who takes copious notes and treats the } machine well; I've had others who take few or no notes and then take an } extremely long time to train properly. } } } ------------------------------------------------------------ } This email message, including any attachments, is for the sole use of the } intended recipient(s) and may contain information that is proprietary, } confidential, and exempt from disclosure under applicable law. Any } unauthorized review, use, disclosure, or distribution is prohibited. If you } have received this email in error please notify the sender by return email and } delete the original message. Please note, the recipient should check this } email and any attachments for the presence of viruses. The organization } accepts no liability for any damage caused by any virus transmitted by this } email. } ================================= } } } } ==============================Original Headers============================== } 7, 32 -- From Michael.Cammer-at-med.nyu.edu Wed Mar 2 07:21:31 2011 } 7, 32 -- Received: from mail3.nyumc.org (mail3.nyumc.org [216.165.126.230]) } 7, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p22DLS0F010477 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 07:21:29 -0600 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-Anti-Spam-Result: } AusAAFPTbU0KkyXl/2dsb2JhbACYAY9KtGuIa4VhBIUXkyM } 7, 32 -- X-IronPort-AV: E=Sophos;i="4.62,253,1297054800"; } 7, 32 -- d="scan'208";a="123455669" } 7, 32 -- Received: from msgwsdcpht63.nyumc.org ([10.147.37.229]) } 7, 32 -- by mail3.nyumc.org with SMTP; 02 Mar 2011 08:21:27 -0500 } 7, 32 -- Received: from MSGWSDCPMB06.nyumc.org ([10.147.55.93]) by } 7, 32 -- MSGWSDCPHT63.nyumc.org ([10.147.37.229]) with mapi; Wed, 2 Mar 2011 } 08:21:28 } 7, 32 -- -0500 } 7, 32 -- From: "Cammer, Michael" {Michael.Cammer-at-med.nyu.edu} } 7, 32 -- To: "'jsb43-at-cam.ac.uk'" {jsb43-at-cam.ac.uk} } 7, 32 -- CC: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 7, 32 -- Date: Wed, 2 Mar 2011 08:21:26 -0500 } 7, 32 -- Subject: RE: [Microscopy] Re: Question for university facility } 7, 32 -- Thread-Topic: [Microscopy] Re: Question for university facility } 7, 32 -- Thread-Index: AcvYuxJp7TA4cHxqSEqPkSQSBE+PywAIIBNQ } 7, 32 -- Message-ID: } {725986F6C4F57449A27E7D10B49F82B33A019CFB47-at-MSGWSDCPMB06.nyumc.org} } 7, 32 -- References: {201103020920.p229KQpa027206-at-ns.microscopy.com} } 7, 32 -- In-Reply-To: {201103020920.p229KQpa027206-at-ns.microscopy.com} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- acceptlanguage: en-US } 7, 32 -- Content-Type: text/plain; charset="us-ascii" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p22DLS0F010477 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 31 -- From dsherman-at-purdue.edu Wed Mar 2 07:39:13 2011 7, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22DdDw4026781 7, 31 -- for {microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 07:39:13 -0600 7, 31 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 7, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p22DdDqM005805 7, 31 -- for {microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 08:39:13 -0500 7, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB04H.purdue.lcl 7, 31 -- ([::1]) with mapi; Wed, 2 Mar 2011 08:39:12 -0500 7, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 7, 31 -- To: "Michael.Cammer-at-med.nyu.edu" {Michael.Cammer-at-med.nyu.edu} , 7, 31 -- "message to: 7, 31 -- MSA list" {microscopy-at-microscopy.com} 7, 31 -- Date: Wed, 2 Mar 2011 08:39:10 -0500 7, 31 -- Subject: Re: [Microscopy] Question for university facility 7, 31 -- Thread-Topic: [Microscopy] Question for university facility 7, 31 -- Thread-Index: AcvY3QfRdLRIU1F9Siuxp5Lm1goCqgAAi3a5 7, 31 -- Message-ID: {C993B2AE.C06F%dsherman-at-purdue.edu} 7, 31 -- In-Reply-To: {201103021323.p22DNTkH013286-at-ns.microscopy.com} 7, 31 -- Accept-Language: en-US 7, 31 -- Content-Language: en-US 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 7, 31 -- acceptlanguage: en-US 7, 31 -- Content-Type: text/plain; charset="us-ascii" 7, 31 -- MIME-Version: 1.0 7, 31 -- X-PMX-Version: 5.5.9.388399 7, 31 -- X-PerlMx-Virus-Scanned: Yes 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p22DdDw4026781 ==============================End of - Headers==============================
We do quite a bit of pigment analysis here using multiple techniques, starting with polarized light microscopy. This can often be definitive, but SEM is typically used for confirmation of elemental composition. Electron microscopy can be especially useful for analysis of smaller particles that might be present in the pigment, and for detection of trace elements. Transmission electron microscopy is not used as often here as SEM, but is valuable in that it provides information about particle morphology, elemental composition and crystalline phase on even the smallest particles in a sample. Even if two pigments have similar elemental compositions, higher resolution electron microscopy techniques may provide information about variations in elemental ratios, presence of trace elements or minor phases, and information about particle size and morphology or crystalline phase that will be of help in identifying the pigment or determining whether it is a natural or synthetic material, if that's of interest to your researcher. You may also want to consider the nature of the samples; are they pure pigments, or have they been sampled from an object or a substrate that might contribute other types of particulate? I would advise starting with PLM, and using SEM and/or TEM from there to confirm and expand upon the PLM results.
Title-Subject: [Filtered] electron microscopy and pigments
Message: Hi,
I have a researcher interested in identifying pigments from a sample. I cannot think of a way we can use TEM or SEM to identify these pigments. I think that all the pigments within the sample will have similar composition of elements and I don't think we will be able to distinguish them. Has anyone ever looked at identifying pigments from their specimens maybe a method we could try?
Sincerely, Angel Paredes
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. ********************************************************************* *** DATA PROVIDED IN THIS E-MAIL IS CONSIDERED PRELIMINARY AND IS SUBJECT TO REVISION. A FULL AND COMPLETE REPORT OF FINAL FINDINGS WILL BE DELIVERED IN HARD COPY VIA MAIL OR OTHER COURIER.
==============================Original Headers============================== 19, 25 -- From eschumacher-at-mccrone.com Wed Mar 2 07:53:22 2011 19, 25 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 19, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22DrMET010698 19, 25 -- for {microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 07:53:22 -0600 19, 25 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 19, 25 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Wed, 2 Mar 2011 07:53:20 19, 25 -- -0600 19, 25 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 19, 25 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 19, 25 -- "Angel.Paredes-at-fda.hhs.gov" {Angel.Paredes-at-fda.hhs.gov} 19, 25 -- Date: Wed, 2 Mar 2011 07:53:19 -0600 19, 25 -- Subject: Re: Electron Microscopy and Pigments 19, 25 -- Thread-Topic: Re: Electron Microscopy and Pigments 19, 25 -- Thread-Index: AcvY4TAD6d60PrZRSBOU0No1VK2axw== 19, 25 -- Message-ID: {874B1DB532886444A60A015EE363493F484C970459-at-mccronemsg07.tmg.mccrone.com} 19, 25 -- Accept-Language: en-US 19, 25 -- Content-Language: en-US 19, 25 -- X-MS-Has-Attach: 19, 25 -- X-MS-TNEF-Correlator: 19, 25 -- x-vipre-scanned: 0A7989D00021860A798B1D 19, 25 -- acceptlanguage: en-US 19, 25 -- Content-Type: text/plain; charset="us-ascii" 19, 25 -- MIME-Version: 1.0 19, 25 -- Content-Transfer-Encoding: 8bit 19, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p22DrMET010698 ==============================End of - Headers==============================
} Email: Angel.Paredes-at-fda.hhs.gov Name: Angel Paredes } } Organization: FDA/NCTR } } Title-Subject: [Filtered] electron microscopy and pigments } } Message: Hi, } } I have a researcher interested in identifying pigments from a sample. I } cannot think of a way we can use TEM or SEM to identify these pigments. } I think that all the pigments within the sample will have similar } composition of elements and I don't think we will be able to distinguish } them. Has anyone ever looked at identifying pigments from their } specimens maybe a method we could try? } } Sincerely, } Angel Paredes
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 26 -- From oshel1pe-at-cmich.edu Wed Mar 2 08:04:13 2011 3, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22E4DJN026847 3, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 08:04:13 -0600 3, 26 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p22E49Iu021246; 3, 26 -- Wed, 2 Mar 2011 09:04:12 -0500 3, 26 -- Received: from [141.209.160.249] ([141.209.160.249]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 3, 26 -- Wed, 2 Mar 2011 09:04:07 -0500 3, 26 -- Mime-Version: 1.0 3, 26 -- Message-Id: {a06240803c993feaab649-at-[141.209.160.249]} 3, 26 -- In-Reply-To: {201103020252.p222qUYX015159-at-ns.microscopy.com} 3, 26 -- References: {201103020252.p222qUYX015159-at-ns.microscopy.com} 3, 26 -- Date: Wed, 2 Mar 2011 09:04:02 -0500 3, 26 -- To: Angel.Paredes-at-fda.hhs.gov 3, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 26 -- Subject: Re: [Microscopy] viaWWW:electron microscopy and pigments 3, 26 -- Cc: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 3, 26 -- X-OriginalArrivalTime: 02 Mar 2011 14:04:07.0725 (UTC) FILETIME=[B26345D0:01CBD8E2] 3, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 26 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 3, 26 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 26 -- X-CanItPRO-Stream: default 3, 26 -- X-Canit-Stats-ID: 02Eee4clJ - 15621109e76c - 20110302 3, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
While I do not have much experience with pigment microscopy, from using pigments for painting I know they can contain Fe, Cr, Cd, Hg, Pb, Cu, Co, Cr... Using EDS alone may not be enough for identifying pigment (Cd containing pigments could be yellow, red, green), but it can be a good starting point. Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Tuesday, March 01, 2011 8:49 PM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW:electron microscopy and pigments } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the } MIcroscopy Listserver } ----------------------------------------------------------------------- } ---- } } Email: Angel.Paredes-at-fda.hhs.gov Name: Angel Paredes } } Organization: FDA/NCTR } } Title-Subject: [Filtered] electron microscopy and pigments } } Message: Hi, } } I have a researcher interested in identifying pigments from a sample. } I } cannot think of a way we can use TEM or SEM to identify these pigments. } I think that all the pigments within the sample will have similar } composition of elements and I don't think we will be able to } distinguish } them. Has anyone ever looked at identifying pigments from their } specimens maybe a method we could try? } } Sincerely, } Angel Paredes } } Login Host: 150.148.0.65 } ----------------------------------------------------------------------- } ---- } } } } ==============================Original } Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Mar 1 } 20:48:27 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p222mRE4004951 } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 1 Mar 2011 20:48:27 - } 0600 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id AE39D346F32 } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 1 Mar 2011 20:48:27 } -0600 (CST) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 10, 25 -- with ESMTP id LVjjMFwQADRJ for {microscopy-at-microscopy.com} ; } 10, 25 -- Tue, 1 Mar 2011 20:48:27 -0600 (CST) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id 59B0F346F27 } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 1 Mar 2011 20:48:27 } -0600 (CST) } 10, 25 -- Message-ID: {4D6DAFFB.7000301-at-microscopy.com} } 10, 25 -- Date: Tue, 01 Mar 2011 20:48:27 -0600 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 10, 25 -- Reply-To: Angel.Paredes-at-fda.hhs.gov } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: viaWWW:electron microscopy and pigments } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 29 -- From DusevichV-at-umkc.edu Wed Mar 2 09:33:04 2011 5, 29 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22FX38R013636 5, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 09:33:03 -0600 5, 29 -- X-IronPort-Anti-Spam-Filtered: true 5, 29 -- X-IronPort-Anti-Spam-Result: AgEBADfybU3Raua2/2dsb2JhbACYAYcZhmBedLUEiGuCfhsBgkcEhRc 5, 29 -- Received: from um-nhub02.um.umsystem.edu ([209.106.230.182]) 5, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 02 Mar 2011 09:33:03 -0600 5, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.153]) by 5, 29 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Wed, 2 Mar 2011 5, 29 -- 09:33:03 -0600 5, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 29 -- Date: Wed, 2 Mar 2011 09:33:01 -0600 5, 29 -- Subject: RE: [Microscopy] viaWWW:electron microscopy and pigments 5, 29 -- Thread-Topic: [Microscopy] viaWWW:electron microscopy and pigments 5, 29 -- Thread-Index: AcvYhFzdfKKTgYSHTfGOzpizvAGgWgAadBDg 5, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3AE14EA156E-at-UM-EMAIL06.um.umsystem.edu} 5, 29 -- References: {201103020248.p222mniM006106-at-ns.microscopy.com} 5, 29 -- In-Reply-To: {201103020248.p222mniM006106-at-ns.microscopy.com} 5, 29 -- Accept-Language: en-US 5, 29 -- Content-Language: en-US 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- acceptlanguage: en-US 5, 29 -- Content-Type: text/plain; charset="us-ascii" 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p22FX38R013636 ==============================End of - Headers==============================
Dear Angel, This looks like a task for LM. The absorption spectra and/or fluorescence spectra are characteristic of the pigments, and, if there are several pigments in small adjacent areas, you could measure the spectra. Of course, some pigments, like cobalt blue, could be identified from EDS, but your post suggests that you have organic pigments to deal with. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
Title-Subject: [Filtered] electron microscopy and pigments
Message: Hi,
I have a researcher interested in identifying pigments from a sample. I cannot think of a way we can use TEM or SEM to identify these pigments. I think that all the pigments within the sample will have similar composition of elements and I don't think we will be able to distinguish them. Has anyone ever looked at identifying pigments from their specimens maybe a method we could try?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22I9953026259 for {MicroscopyListserverArchive2-at-microscopy.com} ; Wed, 2 Mar 2011 12:09:09 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p22I99p9026257; Wed, 2 Mar 2011 12:09:09 -0600
Angel,
FTIR and possibly FTIR Microscopy can also be helpful in identifying pigments (as long as they aren't filled with carbon black). There is an excellent FTIR reference on this topic: "High Resolution Spectra of Inorganic Pigments and Extenders In the Mid-Infrared Region From 1500 cm-1 to 200 cm-1" by Leonard C. Afremow and John T. Vandeberg. This was published in the Journal of Paint Technology , Vol 38, No 495, April 1966.
Kristin A. Breen Chemist, Analytical Sciences Laboratory ExxonMobil Research and Engineering Paulsboro Technical Center Paulsboro, NJ 08066 (856) 224-2864
----- Forwarded by Kristin A Breen/EastCoast/Mobil-Notes on 03/02/2011 01:05 PM -----
William.F.Tivo l-at-aero.org To kristin.a.breen-at-exxonmobil.com 03/02/2011 cc 12:21 PM Subject [Microscopy] Re: viaWWW:electron Please respond microscopy and pigments to William.F.Tivo l-at-aero.org
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Angel, This looks like a task for LM. The absorption spectra and/or fluorescence spectra are characteristic of the pigments, and, if there are several pigments in small adjacent areas, you could measure the spectra. Of course, some pigments, like cobalt blue, could be identified from EDS, but your post suggests that you have organic pigments to deal with. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
Title-Subject: [Filtered] electron microscopy and pigments
Message: Hi,
I have a researcher interested in identifying pigments from a sample. I cannot think of a way we can use TEM or SEM to identify these pigments. I think that all the pigments within the sample will have similar composition of elements and I don't think we will be able to distinguish them. Has anyone ever looked at identifying pigments from their specimens maybe a method we could try?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22INAkc010144 for {MicroscopyListserverArchive2-at-microscopy.com} ; Wed, 2 Mar 2011 12:23:10 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p22INAR7010142; Wed, 2 Mar 2011 12:23:10 -0600
Angel et al,
The folks at McCrone Research have done extensive studies of pigments using polarized light and other LM techniques. Perhaps you could contact them ( http://www.mcri.org/home/ ) for additional insight.
Jeff Stewart Metallographic Lab Manager Cookson Precious Metals 49 Pearl Street Attleboro, MA 02703 508-222-7400 x1329
On Wed Mar 2 12:22 , William.F.Tivol-at-aero.org sent:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 32 -- From SRS0=pTv1rR=V3=metallography.com=jeff-at-eigbox.net Wed Mar 2 12:23:02 2011 8, 32 -- Received: from bosmailout14.eigbox.net (bosmailout14.eigbox.net [66.96.186.14]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22IN2vx010131 8, 32 -- for {microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 12:23:02 -0600 8, 32 -- Received: from bosmailscan07.eigbox.net ([10.20.15.7]) 8, 32 -- by bosmailout14.eigbox.net with esmtp (Exim) 8, 32 -- id 1PuqhR-0006Af-R5 8, 32 -- for microscopy-at-microscopy.com; Wed, 02 Mar 2011 13:23:01 -0500 8, 32 -- Received: from bosimpout02.eigbox.net ([10.20.55.2]) 8, 32 -- by bosmailscan07.eigbox.net with esmtp (Exim) 8, 32 -- id 1PuqhR-0006HG-53 8, 32 -- for microscopy-at-microscopy.com; Wed, 02 Mar 2011 13:23:01 -0500 8, 32 -- Received: from boswebmail23.eigbox.net ([10.20.16.23]) 8, 32 -- by bosimpout02.eigbox.net with NO UCE 8, 32 -- id EJP11g00E0VrLTu01JP1s8; Wed, 02 Mar 2011 13:23:01 -0500 8, 32 -- X-EN-OrigOutIP: 10.20.16.23 8, 32 -- X-EN-IMPSID: EJP11g00E0VrLTu01JP1s8 8, 32 -- Received: from [69.3.202.194] by boswebmail23.eigbox.net via HTTP; Wed, 02 Mar 2011 13:23:01 -0500 8, 32 -- Content-Disposition: inline 8, 32 -- Content-Type: text/plain; charset="iso-8859-1" 8, 32 -- MIME-Version: 1.0 8, 32 -- From: {jeff-at-metallography.com} 8, 32 -- To: Microscopy-at-microscopy.com 8, 32 -- Subject: Re: [Microscopy] Re: viaWWW:electron microscopy and pigments 8, 32 -- Reply-To: jeff-at-metallography.com 8, 32 -- X-Origin: 69.3.202.194 8, 32 -- Date: Wed, 02 Mar 2011 13:22:51 -0500 8, 32 -- Message-Id: {54300.1299090171-at-metallography.com} 8, 32 -- X-Mailer: AtMail 4.61 - 69.3.202.194 - jeff-at-metallography.com 8, 32 -- Sender: {jeff-at-metallography.com} 8, 32 -- Content-Transfer-Encoding: 8bit 8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p22IN2vx010131 ==============================End of - Headers==============================
I note that there is also going to be a Symposium at MM2011 (http://microscopy.org/MandM/2011/program/symposia.cfm) entitled
P07 Microscopy and Microanalysis Applications in Cultural Heritage Research
Quoting from the description of the symposium
"This symposium will focus on where the application of microscopy and microanalysis techniques can aid cultural heritage research, principally in the areas of conservation, maintenance, provenance and restoration. Materials of study may include: Metals; ceramics (porcelain and pottery); building materials (stone, brick and mortar); glass; textiles; paper; paint and pigments; mineralogy; coinage and jewelry."
This should be an excellent place to get more information about using microscopy in the study of pigments.
Nestor Your Friendly Neighborhood SysOp
On 3/1/11 8:48 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Angel.Paredes-at-fda.hhs.gov Name: Angel Paredes } } Organization: FDA/NCTR } } Title-Subject: [Filtered] electron microscopy and pigments } } Message: Hi, } } I have a researcher interested in identifying pigments from a sample. I } cannot think of a way we can use TEM or SEM to identify these pigments. } I think that all the pigments within the sample will have similar } composition of elements and I don't think we will be able to distinguish } them. Has anyone ever looked at identifying pigments from their } specimens maybe a method we could try? } } Sincerely, } Angel Paredes } } Login Host: 150.148.0.65 } --------------------------------------------------------------------------- } } } }
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory President Microscopy Society of America
I have been very interested to read all the comments about users misusing instruments. I managed the central electron microscopy facility here at the University of Michigan for over twenty five years, and came to the conclusion that some people just aren't built to work with delicate instruments, while others take to the task just naturally. This reminds me of the following story that I recall telling in my Presidential Address at the annual EMSA banquet back in 1969 to illustrate this problem:
It seems that two students were talking over coffee in the student lounge; First Student: "I hear you are raising parakeets; how is the project working out?"
Second Student: "Fine, except that one bird keeps pecking the other birds. I'm afraid he'll end up seriously injuring one of them if I can't stop him"
First student: "That shouldn't be a problem. My grandmother raised parakeets, and when one of them did that she stopped it by filing just a bit off the end of his beak."
A week later the two students meet again:
First student: "Did filing the beak on your aggressive parakeet solve your problem?"
Second student: "No, it killed him!"
First student: "Wow, it shouldn't have done that!"
Second student: "Well, it did! He was dead when I took his head out of the vice".
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 12, 15 -- From bigelow-at-umich.edu Wed Mar 2 14:01:41 2011 12, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 12, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22K1fWT013809 12, 15 -- for {microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 14:01:41 -0600 12, 15 -- Received: FROM [76.226.72.28] (adsl-76-226-72-28.dsl.sfldmi.sbcglobal.net [76.226.72.28]) 12, 15 -- By hackers.mr.itd.umich.edu ID 4D6EA224.AD22E.20961 ; 12, 15 -- Authuser bigelow; 12, 15 -- 2 Mar 2011 15:01:40 EST 12, 15 -- Mime-Version: 1.0 12, 15 -- Message-Id: {p06240801c9944eb11b58-at-[76.226.72.28]} 12, 15 -- Date: Wed, 2 Mar 2011 15:00:43 -0500 12, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 12, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 12, 15 -- Subject: [Microscopy] RE: instrument damage 12, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
Use Polarized Light Microscopy on picked out pigment particles.
See:
1. McCrone: The Microscopical ID of artists Pigments, J Int Inst Conserv Can Group, 17, 1-2, 11-34, 1982. (BEST - PLM, SEM-EDX)
2. Schreiner: SEM and energy dispersive (EDX) anal appl in the field of cultural heritage, Anal Bioanal Chem, 387, 737-747, 2007. (SEM-EDX)
3. Banik: Microchemical characterization of paintings - A case study, Microchim acta ,76, 1-2, 93-109, 1981. (SEM-EDX FTIR)
There are others but these provide a good start.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123 www.ph2llc.com (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
==============================Original Headers============================== 15, 27 -- From ph2-at-sprynet.com Wed Mar 2 15:12:15 2011 15, 27 -- Received: from elasmtp-kukur.atl.sa.earthlink.net (elasmtp-kukur.atl.sa.earthlink.net [209.86.89.65]) 15, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p22LCFjm031844 15, 27 -- for {microscopy-at-microscopy.com} ; Wed, 2 Mar 2011 15:12:15 -0600 15, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 15, 27 -- s=dk20050327; d=sprynet.com; 15, 27 -- b=AYoj3XW3aMcitiX3Z+yX6EC2e5kaAi6iTlqvjK+5ZBDQLJAyM+V2LL9KBE2GxIsd; 15, 27 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP; 15, 27 -- Received: from [75.61.18.94] (helo=AAHPC) 15, 27 -- by elasmtp-kukur.atl.sa.earthlink.net with esmtpa (Exim 4.67) 15, 27 -- (envelope-from {ph2-at-sprynet.com} ) 15, 27 -- id 1PutLC-0007ZI-NQ 15, 27 -- for microscopy-at-microscopy.com; Wed, 02 Mar 2011 16:12:14 -0500 15, 27 -- From: "Tony Havics" {ph2-at-sprynet.com} 15, 27 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com} 15, 27 -- Subject: Re: Electron microscopy and pigments 15, 27 -- Date: Wed, 2 Mar 2011 16:12:09 -0500 15, 27 -- Message-ID: {012f01cbd91e$7e6314e0$7b293ea0$-at-com} 15, 27 -- MIME-Version: 1.0 15, 27 -- Content-Type: text/plain; 15, 27 -- charset="us-ascii" 15, 27 -- Content-Transfer-Encoding: 7bit 15, 27 -- X-Mailer: Microsoft Office Outlook 12.0 15, 27 -- Thread-Index: AcvZAF29UUOCkcm3QpesPXlzXWnzTw== 15, 27 -- Content-Language: en-us 15, 27 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f959eac29ad4c12903ae1228664d339dcc350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c 15, 27 -- X-Originating-IP: 75.61.18.94 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both smythen-at-musc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of SC
Title-Subject: [Filtered] epon etching
Message: Years ago I cut my plastic sections and etched them to do other than toludine blue staining. I can't find the procedure in the lab now and was wondering if anyone had a procedure they are willing to share. I know I cut and dried the sections as usual. Then I used a strong sodium hydroxide, but how strong and how long I can't remember.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both pmccurdy-at-lamar.colostate.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: pmccurdy-at-lamar.colostate.edu Name: Patrick McCurdy
Organization: CSU
Title-Subject: [Filtered] AFM tips
Message: Recently we our facility acquired an AFM. Our stockroom has agreed to stock a tapping mode tip and a contact tip. My question is what is the best generic tips if we only stock one of each kind.
While fighting our current 119 cm (3.9 feet) of snow (and counting) my mind began to wander and I became curious about the distribution of magnification in the images we've acquired with our (tungsten-based) SEM. Since we've logged all the images it wasn't hard to find out:
http://www.mta.ca/dmf/sem_mag_dist.html
We do SEM for a lot of different departments, but the bulk of our work is biological, with heavy emphasis on microorganisms, particularly diatoms. My questions to others out there who might have been in similar circumstances are:
1. Do you feel that this sort of distribution is determined more by the instrument capabilities, or the inherent properties of the specimens observed? 2. If we were go for a ~5-10X $$$$ investment in a field emission scope a) would this distribution of magnifications change significantly and b) would the upgrade be demonstrably worthwhile?
I realize there is a big pile of ifs/ands/buts in these questions - I'm just looking for people who are willing to share thoughts, particularly if they have experience in taking this route.
Thanks as always,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
The enhanced capabilities of a FESEM, especially if it also has an in-lens detector and perhaps EDX and/or cryo capabilities, will not only do all that your present instruments does, but lots more. Thus the prospect of many new users who would find less satisfactory results with your present instrument. Larger user base means increased use and thus more revenue that hopefully will cover significantly increased service contract cost.
However, I would encourage holding on to your other scope if at all possible. It would make a great training scope and useful in its current role of lower magnification imaging.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "jehrman-at-mta.ca" {jehrman-at-mta.ca} } Reply-To: "jehrman-at-mta.ca" {jehrman-at-mta.ca} } Date: Thu, 3 Mar 2011 09:34:43 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] mag distribution of SEM images } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Listers: Good Day, eh? } } While fighting our current 119 cm (3.9 feet) of snow (and counting) my } mind began to wander and I became curious about the distribution of } magnification in the images we've acquired with our (tungsten-based) } SEM. Since we've logged all the images it wasn't hard to find out: } } http://www.mta.ca/dmf/sem_mag_dist.html } } We do SEM for a lot of different departments, but the bulk of our work } is biological, with heavy emphasis on microorganisms, particularly } diatoms. My questions to others out there who might have been in similar } circumstances are: } } 1. Do you feel that this sort of distribution is determined more by the } instrument capabilities, or the inherent properties of the specimens } observed? } 2. If we were go for a ~5-10X $$$$ investment in a field emission scope } a) would this distribution of magnifications change significantly and b) } would the upgrade be demonstrably worthwhile? } } I realize there is a big pile of ifs/ands/buts in these questions - I'm } just looking for people who are willing to share thoughts, particularly } if they have experience in taking this route. } } Thanks as always, } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } After twelve years of therapy my } psychiatrist said something that } brought tears to my eyes. } He said, "No hablo ingles." } } } ==============================Original Headers============================== } 13, 19 -- From jehrman-at-mta.ca Thu Mar 3 08:32:27 2011 } 13, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.114]) } 13, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p23EWQhc019747 } 13, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 08:32:26 -0600 } 13, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:54880) } 13, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) } 13, 19 -- (Exim 4.71) } 13, 19 -- (envelope-from {jehrman-at-mta.ca} ) } 13, 19 -- id 1Pv9Zq-0007BL-Bi } 13, 19 -- for Microscopy-at-microscopy.com; Thu, 03 Mar 2011 10:32:26 -0400 } 13, 19 -- Message-ID: {4D6FA677.80503-at-mta.ca} } 13, 19 -- Date: Thu, 03 Mar 2011 10:32:23 -0400 } 13, 19 -- From: James Ehrman {jehrman-at-mta.ca} } 13, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; } rv:1.9.2.14) Gecko/20110221 Thunderbird/3.1.8 } 13, 19 -- MIME-Version: 1.0 } 13, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} } 13, 19 -- Subject: mag distribution of SEM images } 13, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 19 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 31 -- From dsherman-at-purdue.edu Thu Mar 3 09:27:10 2011 7, 31 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23FRA7L005002 7, 31 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 09:27:10 -0600 7, 31 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 7, 31 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p23FR8Ot031872; 7, 31 -- Thu, 3 Mar 2011 10:27:08 -0500 7, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB04H.purdue.lcl 7, 31 -- ([::1]) with mapi; Thu, 3 Mar 2011 10:27:08 -0500 7, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 7, 31 -- To: "jehrman-at-mta.ca" {jehrman-at-mta.ca} , 7, 31 -- "message to: MSA list" 7, 31 -- {microscopy-at-microscopy.com} 7, 31 -- Date: Thu, 3 Mar 2011 10:27:05 -0500 7, 31 -- Subject: Re: [Microscopy] mag distribution of SEM images 7, 31 -- Thread-Topic: [Microscopy] mag distribution of SEM images 7, 31 -- Thread-Index: AcvZsCQMT4Mc1+pDTbe7W12Wcm8srwAB09ys 7, 31 -- Message-ID: {C9951D79.C12E%dsherman-at-purdue.edu} 7, 31 -- In-Reply-To: {201103031434.p23EYhC7022550-at-ns.microscopy.com} 7, 31 -- Accept-Language: en-US 7, 31 -- Content-Language: en-US 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 7, 31 -- acceptlanguage: en-US 7, 31 -- Content-Type: text/plain; charset="us-ascii" 7, 31 -- MIME-Version: 1.0 7, 31 -- X-PMX-Version: 5.5.9.388399 7, 31 -- X-PerlMx-Virus-Scanned: Yes 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p23FRA7L005002 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrew.d.vogt-at-abbott.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Abbott is seeking a summer intern student in its Global Pharmaceutical R&D Division to apply atomic force microscopy towards an understanding of single-particle interactions and phase-separated materials. A candidate should be enthusiastic, self motivating, works well on teams, has excellent writing and communication skills, as well as has a strong desire to carry out an aggressive research project. This student should have a basic knowledge of chemical principles, materials science and will be enrolled in school in Fall 2011. Experience using atomic force microscopy is strongly preferred. To apply, visit www.abbott.com/careers, click student and internship program. Look for Science Intern and apply directly.
Jim, Thanks for the information. As I've had posted on my website for years, "Our experience has been that many SEMs are typically operated at or below 2,500X and rarely over 10,000X. If you don't require the very high resolution of field emission, the capabilities of an environmental SEM or the ability to remotely operate the system, your familiar and faithful SEM may still be your best friend."
Debbie has a point that the FESEM may open up new users, but x-ray still has volume limitations and low kV analysis still has limitations, particularly if you want quantitative results. My feeling is that biological, geological and failure analysis users are probably not going to be big users of the new capabilities. The big users will most likely be microelectronics, MEMS and nano-tube/particle studies. If these folks are in your system, they will love you.
If you can further parse your data, it would be interesting to see the breakdown of users at 10kx and up. Butterfly scales are fun, but how many micrographs of diatoms are in that range vs ICs and nanoparticles?
I'd love to hear from more people about this because generally it was only my IC customers who pushed mag all the time.
Our local ski area is claiming over 100" (254cm) so far for the season, but I think they get all their data from the summit. My yard is still looking like a good winter, though. I was never fond of those brown and gray winters that we generally had in south-central Pennsylvania. It's bright and beautiful outside my windows today.
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] Sent: Thursday, March 03, 2011 9:35 AM To: kenconverse-at-qualityimages.biz
Listers: Good Day, eh?
While fighting our current 119 cm (3.9 feet) of snow (and counting) my mind began to wander and I became curious about the distribution of magnification in the images we've acquired with our (tungsten-based) SEM. Since we've logged all the images it wasn't hard to find out:
http://www.mta.ca/dmf/sem_mag_dist.html
We do SEM for a lot of different departments, but the bulk of our work is biological, with heavy emphasis on microorganisms, particularly diatoms. My questions to others out there who might have been in similar circumstances are:
1. Do you feel that this sort of distribution is determined more by the instrument capabilities, or the inherent properties of the specimens observed? 2. If we were go for a ~5-10X $$$$ investment in a field emission scope a) would this distribution of magnifications change significantly and b) would the upgrade be demonstrably worthwhile?
I realize there is a big pile of ifs/ands/buts in these questions - I'm just looking for people who are willing to share thoughts, particularly if they have experience in taking this route.
Thanks as always,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
If you are doing single molecule fluorescence microscopy (TIRF for example) on a stationary specimen, how would you determine the expected image of that single molecule in the camera given the shape of the Airy disk, the magnification/NA of hte objective, camera pixel size etc. I am trying to understand whether the Airy diffraction pattern would all be present in a single pixel or whether the central maximum is more than a single pixel and whether the higher orders are visible. Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Thu Mar 3 10:50:41 2011 5, 19 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23GofWv022660 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 10:50:41 -0600 5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) 5, 19 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 995902D0AC 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 11:50:41 -0500 (EST) 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=us-ascii 5, 19 -- Subject: Single molecule imaging 5, 19 -- Date: Thu, 3 Mar 2011 11:50:41 -0500 5, 19 -- Message-Id: {6368476E-325C-4ADD-AAB9-8B0093116EE0-at-uconn.edu} 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 19 -- X-Mailer: Apple Mail (2.1082) 5, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 5, 19 -- X-Virus-Status: Clean 5, 19 -- Content-Transfer-Encoding: 8bit 5, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p23GofWv022660 ==============================End of - Headers==============================
could you please forward this email to anybody who might be interested in this position?
Thanks Robert
Research Assistant Professor Position in Aberration-Corrected Scanning Transmission Electron Microscopy
**An Research Assistant Professor position is available in the Nanoscale Physics Group lead by Professor Robert F. Klie at the University of Illinois at Chicago to work on the application of atomic-resolution Z-contrast and annular bright-field imaging, as well as electron energy-loss spectroscopy (EELS) to functional nano-materials. The successful candidate will working with the group’s new aberration-corrected cold-field emission STEM (the JEOL ARM200-CF) with sub-Å and sub-eV resolution, equipped with several in-situ holders.
Candidates should have a Ph.D. in Physics, Materials Science or a related discipline. The position requires extensive experience in transmission electron microscopy and electron energy-loss spectroscopy. Experience working with aberration-corrected scanning transmission electron microscopy is also preferred. This position is available starting July 1, 2011, and remains open until it is filled. For fullest consideration apply by May 15, 2011. The salary will range between $55,000 and $75,000 depending on the level of experience and seniority.
Interested candidates can apply online at http://jobs.uic.edu. Please submit a cover letter, CV with a complete list of publications, and contact information of three professional references. Questions about the position should be directed to the search committee chair (Prof. Robert Klie; rfklie-at-uic.edu). UIC is an AA/EOE
-- Robert F. Klie, PhD Assistant Professor University of Illinois at Chicago Department of Physics Chicago, IL 60607 Tel: 312-996-6064 Fax: 312-996-9016
Editor Journal of Undergraduate Research Website {http://jur.phy.uic.edu}
Past President Midwest Microscopy and Microanalysis Society (M^3 S) Website {http://midwestmicroscopy.org}
==============================Original Headers============================== 14, 18 -- From rfklie-at-uic.edu Thu Mar 3 11:07:15 2011 14, 18 -- Received: from mail-3.cc.uic.edu (mail-3.cc.uic.edu [128.248.155.42]) 14, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23H7FKA006646 14, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 11:07:15 -0600 14, 18 -- Received: from [131.193.192.91] (klielaptop.phy.uic.edu [131.193.192.91]) 14, 18 -- (authenticated bits=0) 14, 18 -- by mail-3.cc.uic.edu (8.14.4/8.14.4) with ESMTP id p23H7EJu005442 14, 18 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 14, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 11:07:15 -0600 14, 18 -- Message-ID: {4D6FCAC1.50602-at-uic.edu} 14, 18 -- Date: Thu, 03 Mar 2011 11:07:13 -0600 14, 18 -- From: Robert Klie {rfklie-at-uic.edu} 14, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 14, 18 -- MIME-Version: 1.0 14, 18 -- To: Microscopy-at-microscopy.com 14, 18 -- Subject: Job opening in aberration corrected STEM at UIC 14, 18 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 18 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Use the Rayleigh criterion to calculate how large the central maximum peak of Airy disk (of a sub-diffraction sized object) will appear for a given objective numerical aperture (NA) and emission wavelength (lambda). Suppose you're imaging the single-molecule fluorescence through a bandpass filter which is say 50 nm wide. Take the central wavelength of this bandpass to represent lambda. Alternatively, you can take lambda to be the known peak wavelength in the emission spectrum of the fluorescent molecule
The Rayleigh criterion says the width of the Airy disk peak will be
r = 1.22(lambda)/2NA
For example, let's say I'm using a 60X, 1.45 NA TIRF objective, and the central wavelength of my bandpass is 500 nm. In this case, r would be ~ 210 nm.
Now, to determine how big the image of the Airy disk appears on your camera, you need to determine the back-projected size of your camera pixels on the sample image plane. This can be estimated by dividing the physical pixel size on the camera chip by the magnification of the objective. Again, as an example, suppose you're using an EMCCD camera which uses the E2V chip which I think has pixels with a physical size of 16x16 micrometers. With the same objective (60X, 1.45 NA), the back-projected size of the pixels on the sample plane is (16/60) ~ 267 nm x 267 nm.
In this example, you can see that your Airy disk pattern, at least the central peak, will only span about 1 pixel across. If the molecule happens to sit on a position between two pixels, you'll see signal in two adjacent pixels (or 4x4 if you include both dimensions). It's been my experience that the other side lobes of the Airy disk are not usually observed in single-molecule imaging because they are much weaker in intensity.
This example also points out why some researchers usually add some additional magnification into the imaging system (either by an optovar lens or a 1.5X-2.0X lens included in the microscope body). For some single-molecule techniques, such as FIONA ("fluorescence imaging with one nanometer accuracy") or PALM/STORM imaging, you need to sample the Airy disk patter just a bit more so that a proper fitting of the pattern can be done to permit highly accurate localization precision.
There might be other ways to do this calculation as well. I would add one note of caution. The NA and magnification printed out the outside barrel of your objective are not always exactly what they say they are. There is some variation from objective to objective of the same make and model. There are ways to measure both parameters. Contact me offline if you want the details. Take the method described above to give you only a ballpark idea.
John Oreopoulos Research Assistant Spectral Applied Research Richmond Hill, Ontario Canada www.Spectral.ca
On 2011-03-03, at 11:55 AM, david.knecht-at-uconn.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } If you are doing single molecule fluorescence microscopy (TIRF for example) on a stationary specimen, how would you determine the expected image of that single molecule in the camera given the shape of the Airy disk, the magnification/NA of hte objective, camera pixel size etc. I am trying to understand whether the Airy diffraction pattern would all be present in a single pixel or whether the central maximum is more than a single pixel and whether the higher orders are visible. Thanks- Dave } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } } } } } ==============================Original Headers============================== } 5, 19 -- From david.knecht-at-uconn.edu Thu Mar 3 10:50:41 2011 } 5, 19 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23GofWv022660 } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 10:50:41 -0600 } 5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) } 5, 19 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 995902D0AC } 5, 19 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 11:50:41 -0500 (EST) } 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} } 5, 19 -- Content-Type: text/plain; charset=us-ascii } 5, 19 -- Subject: Single molecule imaging } 5, 19 -- Date: Thu, 3 Mar 2011 11:50:41 -0500 } 5, 19 -- Message-Id: {6368476E-325C-4ADD-AAB9-8B0093116EE0-at-uconn.edu} } 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 5, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) } 5, 19 -- X-Mailer: Apple Mail (2.1082) } 5, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 5, 19 -- X-Virus-Status: Clean } 5, 19 -- Content-Transfer-Encoding: 8bit } 5, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p23GofWv022660 } ==============================End of - Headers==============================
==============================Original Headers============================== 16, 28 -- From john.oreopoulos-at-utoronto.ca Thu Mar 3 11:22:05 2011 16, 28 -- Received: from bureau62.ns.utoronto.ca (bureau62.ns.utoronto.ca [128.100.132.159]) 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23HM5b2006424 16, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 11:22:05 -0600 16, 28 -- Received: from [192.168.2.13] (bas3-toronto06-2925097395.dsl.bell.ca [174.89.113.179]) 16, 28 -- (authenticated bits=0) 16, 28 -- by bureau62.ns.utoronto.ca (8.13.8/8.13.8) with ESMTP id p23HLuFW020052 16, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NOT); 16, 28 -- Thu, 3 Mar 2011 12:21:58 -0500 16, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=utoronto.ca; s=beta; 16, 28 -- t=1299172919; bh=TPnW0+Wyj/d/C2q7Y/qkv/4y3KKc8OtI0kkeZG/8fz4=; 16, 28 -- h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date: 16, 28 -- Content-Transfer-Encoding:Message-Id:References:To; 16, 28 -- b=ZDOe4QvDAbUuogxloa+JPGGIxa33QCvtAnzKw2W0l1fF9lz6WvzAAKc3MgOlpkBp0 16, 28 -- 2HD5Hl4cN1SBIwpyQgyBHEcU5a//bQTtcwf0Yo0NlKQX2uXJ5A0bU2dov+U1P1XSdU 16, 28 -- qPXxCzja6raOezZo0oYEGGeC1o4hNvvy+zPxQX5U= 16, 28 -- Content-Type: text/plain; charset=us-ascii 16, 28 -- Mime-Version: 1.0 (Apple Message framework v1082) 16, 28 -- Subject: Re: [Microscopy] Single molecule imaging 16, 28 -- From: John Oreopoulos {john.oreopoulos-at-utoronto.ca} 16, 28 -- In-Reply-To: {201103031655.p23GtLi1029914-at-ns.microscopy.com} 16, 28 -- Date: Thu, 3 Mar 2011 12:21:56 -0500 16, 28 -- Message-Id: {927EA250-8AFB-408F-9B7F-243D7E03D560-at-utoronto.ca} 16, 28 -- References: {201103031655.p23GtLi1029914-at-ns.microscopy.com} 16, 28 -- To: David Knecht-charter {david.knecht-at-uconn.edu} , Microscopy-at-microscopy.com 16, 28 -- X-Mailer: Apple Mail (2.1082) 16, 28 -- Content-Transfer-Encoding: 8bit 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p23HM5b2006424 ==============================End of - Headers==============================
Build it, and they will come. If you have a SFEG-SEM, then users will also take hi-mag images, when applicable.
Equally important, is having a range of detectors and high-voltages.
-JQuinn
PS: OoO counter started.
} Date: Thu, 3 Mar 2011 08:43:45 -0600 } To: jquinn-at-ms.cc.sunysb.edu } From: jehrman-at-mta.ca } Subject: [Microscopy] mag distribution of SEM images } } Listers: Good Day, eh? } } While fighting our current 119 cm (3.9 feet) of snow (and counting) my } mind began to wander and I became curious about the distribution of } magnification in the images we've acquired with our (tungsten-based) } SEM. Since we've logged all the images it wasn't hard to find out: } } http://www.mta.ca/dmf/sem_mag_dist.html } } We do SEM for a lot of different departments, but the bulk of our work } is biological, with heavy emphasis on microorganisms, particularly } diatoms. My questions to others out there who might have been in similar } circumstances are: } } 1. Do you feel that this sort of distribution is determined more by the } instrument capabilities, or the inherent properties of the specimens } observed? } 2. If we were go for a ~5-10X $$$$ investment in a field emission scope } a) would this distribution of magnifications change significantly and b) } would the upgrade be demonstrably worthwhile? } } I realize there is a big pile of ifs/ands/buts in these questions - I'm } just looking for people who are willing to share thoughts, particularly } if they have experience in taking this route. } } Thanks as always, } } Jim
==============================Original Headers============================== 8, 16 -- From jquinn-at-ms.cc.sunysb.edu Thu Mar 3 12:51:23 2011 8, 16 -- Received: from mailrelay.stonybrook.edu (mrs.stonybrook.edu [129.49.1.206]) 8, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23IpNEi024609 8, 16 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 12:51:23 -0600 8, 16 -- Received: from happy-b95c68896.ms.cc.sunysb.edu (ceas2064.matscieng.sunysb.edu [129.49.36.224]) 8, 16 -- by mailrelay.stonybrook.edu (8.14.2/8.14.2) with ESMTP id p23IpJ9g020618 8, 16 -- for {microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 13:51:22 -0500 (EST) 8, 16 -- Message-Id: {201103031851.p23IpJ9g020618-at-mailrelay.stonybrook.edu} 8, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 16 -- Date: Thu, 03 Mar 2011 13:51:11 -0500 8, 16 -- To: microscopy-at-microscopy.com 8, 16 -- From: Jim Quinn {jquinn-at-ms.cc.sunysb.edu} 8, 16 -- Subject: Fwd: [Microscopy] mag distribution of SEM images 8, 16 -- Mime-Version: 1.0 8, 16 -- Content-Type: text/plain; charset="us-ascii" 8, 16 -- X-Brightmail-Tracker: AAAAAQAAAUA= ==============================End of - Headers==============================
I'm with Debby. Since we can get better than 1nm resolution and go up to 800,000x mag, we do. Our biologists are seeing things they've never seen before (look at the detail on this image from our S-4800 http://www.sciencemag.org/content/331/6016.cover-expansion ), and we have a lot more engineers coming in to look at thin films for photovoltaic cells, hydrogen fuel cells, etc. Sure, the diatom folks continue to use the lower mags, but I find them sneaking up to look at everything in better detail and perhaps finding new connections between those diatoms and other microorganisms. Hey, we can see bacteriophage erupting from cells in incredible detail. What's not to love? But, try to keep the old standby, too.
X-from Honolulu where there is absolutely no sign of snow and it's 78F, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 3, 22 -- From tina-at-pbrc.hawaii.edu Thu Mar 3 12:55:17 2011 3, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23ItGc7030208 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 12:55:17 -0600 3, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 3, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p23ItFaL009509 3, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 08:55:15 -1000 (HST) 3, 22 -- Received: from localhost (tina-at-localhost) 3, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p23ItFsL009505 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 08:55:15 -1000 (HST) 3, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 3, 22 -- Date: Thu, 3 Mar 2011 08:55:14 -1000 (HST) 3, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 3, 22 -- X-X-Sender: tina-at-b1000 3, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 3, 22 -- Subject: Re: [Microscopy] Re: mag distribution of SEM images 3, 22 -- In-Reply-To: {201103031527.p23FRxdu006321-at-ns.microscopy.com} 3, 22 -- Message-ID: {Pine.GSO.4.64.1103030849000.8978-at-b1000} 3, 22 -- References: {201103031527.p23FRxdu006321-at-ns.microscopy.com} 3, 22 -- MIME-Version: 1.0 3, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
I was wondering if anyone knew of any books or references that give processing protocols designed to preserve specific elements (ie. Ni, Se, etc.) in biological tissues for conducting energy filtered TEM on ultrathin resin sections.
Thanks,
Shannon Modla Research Associate Delaware Biotechnology Institute 15 Innovation Way, Suite 117 Newark, DE 19711
==============================Original Headers============================== 4, 26 -- From modla-at-dbi.udel.edu Thu Mar 3 13:56:57 2011 4, 26 -- Received: from mail.dbi.udel.edu (genome3.dbi.udel.edu [128.175.253.195]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p23Juvix025370 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 13:56:57 -0600 4, 26 -- Received: from localhost (localhost [127.0.0.1]) 4, 26 -- by mail.dbi.udel.edu (Postfix) with ESMTP id 658577C00C 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 14:56:57 -0500 (EST) 4, 26 -- X-Virus-Scanned: amavisd-new at dbi.udel.edu 4, 26 -- Received: from mail.dbi.udel.edu ([127.0.0.1]) 4, 26 -- by localhost (mail.dbi.udel.edu [127.0.0.1]) (amavisd-new, port 10024) 4, 26 -- with ESMTP id 6cwtdJhYeEfH for {Microscopy-at-microscopy.com} ; 4, 26 -- Thu, 3 Mar 2011 14:56:51 -0500 (EST) 4, 26 -- Received: from [128.175.253.84] (host-253-84.nss.udel.edu [128.175.253.84]) 4, 26 -- (using TLSv1 with cipher DHE-RSA-CAMELLIA256-SHA (256/256 bits)) 4, 26 -- (No client certificate requested) 4, 26 -- by mail.dbi.udel.edu (Postfix) with ESMTPS id 2F0167C00A 4, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 14:56:51 -0500 (EST) 4, 26 -- Message-ID: {4D6FF2AA.3000109-at-dbi.udel.edu} 4, 26 -- Date: Thu, 03 Mar 2011 14:57:30 -0500 4, 26 -- From: Shannon Modla {modla-at-dbi.udel.edu} 4, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.14) Gecko/20110221 Thunderbird/3.1.8 4, 26 -- MIME-Version: 1.0 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Subject: EFTEM Sample Prep 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brachfelds-at-mail.montclair.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Job announcement, Electron Microscopy Specialist
Message: Electron microscopy specialist position available, Montclair State University
We seek an electron microscopy specialist to support research and teaching efforts at Montclair State University. The specialist will instruct students, faculty, and visitors in the use of our Hitachi S2460N and S3400N Scanning Electron Microscopes, H7500 Transmission Electron Microscope, and supporting sample preparation equipment. The successful applicant will have expertise in the preparation, imaging, and compositional analysis of natural and synthetic materials, experience with maintenance and trouble-shooting of electron microscopes and their related equipment, and excellent communication and interpersonal skills with the ability to work in a multidisciplinary facility with a wide variety of beginning through advanced users.
Responsibilities of the position include: - Training new users on our three electron microscopes and their supporting equipment - Conducting basic preventative maintenance on the electron microscopes, diagnosing instrument malfunctions, and coordinating with repair personnel - Overseeing chemical hygiene and chemical safety in the electron microscopy facility - Working the facility directors on purchasing and billing - Organizing and teaching workshops and short courses for internal and external users - Participating in university outreach efforts - Soliciting external consulting work and/or soliciting external research funding
A masters degree in a STEM discipline and at least 3 years of experience in the operation and maintenance of electron microscopes and their sample preparation equipment is required. Strong communication skills, significant experience in the preparation, imaging, and analysis of a wide array of sample types is required. Strong knowledge of computers, electronics, and troubleshooting is desirable. The successful candidate will be qualified to conduct consulting work and/or pursue external research funding. Experience with other major analytical equipment is a plus. The preferred start data is August 1, 2011.
A CV, statement of technical skills, research, laboratory management experience and relevant accomplishments, any supplementary materials (reprints, URLs, etc.) and the names and contact information for four references should be sent to brachfelds-at-mail.montclair.edu (email applications preferred). Hard copy applications should be sent to Dr. Stefanie Brachfeld, Department of Earth and Environmental Studies, Box C316-V, Montclair State University, Montclair, NJ 07043. Review of applications will be on March 28, 2011.
All: I invite you explore our web site at http://www.texasmicroscopy.org/ to read the call for papers and see the activities planned for our Spring meeting. Registration and abstract forms are available on the site and students are encouraged to participate.
-- ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Becky Holdford (r-holdford-at-ti.com) 214-567-0360 SC Packaging FA Texas Instruments, Inc. 13536 N. Central Expressway MS 940 Dallas, TX 75243 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
==============================Original Headers============================== 3, 22 -- From r-holdford-at-ti.com Thu Mar 3 19:06:56 2011 3, 22 -- Received: from bear.ext.ti.com (bear.ext.ti.com [192.94.94.41]) 3, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2416u8j030407 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 19:06:56 -0600 3, 22 -- Received: from dlep36.itg.ti.com ([157.170.170.91]) 3, 22 -- by bear.ext.ti.com (8.13.7/8.13.7) with ESMTP id p2416tYB026568 3, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 19:06:56 -0600 3, 22 -- Received: from [156.117.194.82] (localhost [127.0.0.1]) 3, 22 -- by dlep36.itg.ti.com (8.13.8/8.13.8) with ESMTP id p2416taE023243 3, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Mar 2011 19:06:55 -0600 (CST) 3, 22 -- Message-ID: {4D703B2E.2050502-at-ti.com} 3, 22 -- Date: Thu, 03 Mar 2011 19:06:54 -0600 3, 22 -- From: Becky Holdford {r-holdford-at-ti.com} 3, 22 -- Organization: SC Packaging MPI Team 3, 22 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.14) Gecko/20110221 Thunderbird/3.1.8 3, 22 -- MIME-Version: 1.0 3, 22 -- To: MSA Listserver {Microscopy-at-microscopy.com} 3, 22 -- Subject: 2nd Call for Papers for the Texas Society for Microscopy Spring 2011 3, 22 -- meeting 3, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I read with interest your recent posting on magnifications people use in your SEM lab, as well as the comments of other colleagues, and I would like to add a few words: To your first question if magnification is determined more by the instrument capabilities or the inherent properties of the specimens observed, I would say frankly: It's the specimens!
I went through your site and saw your very decent micrographs that combine a fine resolution with a nice depth of field. And saw that your Jeol instrument has a 3-4 nm resolution limit that is not so far from Tina's Hitachi with 1nm resolution. Do you think this can make a great difference in your work?
We all know that to reach these low numbers we need to have a perfectly working source of electrons, a perfectly aligned scope, a perfectly attached and coated specimen (like the ones for resolution assessment) and a perfect focus and stigma etc. Obviously imperfections on these parameters will stop us well before the ultimate resolution, and usually at around 10nm.
Biology microscopists are like detectives, probably more than colleagues from other fields of microscopy. We start from a live organism where the cellular functions including plasma membrane morphology are changing every little fraction of a second. We have to kill this organism as soon as possible and fix it, then to take out the 70 or 80% of water and liquid from around and inside it, and then coat it with metal (if we want to enjoy the clear high resolution images of coated specimens as opposed to native state cells viewed in low vacuum instruments). Then we take images and we try to guess what the morphology can tell as in relation to the cell's function.
To me sounds impossible that after all these torments the morphology of the cell will stay intact at the level of 1 or 2nm that is really the size of big biomolecules. And I think that if I could go so small in cell specimens I would feel really embarrassed: For I would have to describe structures appearing at this level that could be mostly artifacts. For instance the cover on Science magazine that Tina showed us is a microbe with its surface having thousands of tiny grains at the size of a few nanometers. Do these grains represent any real structures or they are artifacts? And if the micrograph is only for decoration, that's fine. But f it is part of the research findings, it could be a trouble for the researcher to comment on it. Nevertheless here I have to admit that my SEM experience is limited to eukaryotic cells.
It's amazing how we tend to get crazy with numbers and magnifications. It happened to me several times to take a high mag picture on a biological specimen (high for me is like 30K) only to find out afterwards that this picture was not any better from a 5K one. For obtaining a nice clear micrograph there are other parameters more important than the instrument's ultimate resolution. Apart from those already mentioned it is also the averaging and oversampling parameters -in other words. how much time the electron beam scans the specimen. And of course the depth of field is critical for the uneven surfaces found in biological samples. For me a real improvement in biology SEM would be a substantial increase in the depth of field, so that more of the specimen can be in focus. However, I have to admit that my knowledge
While writing these lines most of you guys from America are still sleeping, so I hope this morning message will not spoil your mood. Have a nice day from the ever shiny Athens.
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Listers: Good Day, eh?
While fighting our current 119 cm (3.9 feet) of snow (and counting) my mind began to wander and I became curious about the distribution of magnification in the images we've acquired with our (tungsten-based) SEM. Since we've logged all the images it wasn't hard to find out:
http://www.mta.ca/dmf/sem_mag_dist.html
We do SEM for a lot of different departments, but the bulk of our work is biological, with heavy emphasis on microorganisms, particularly diatoms. My questions to others out there who might have been in similar circumstances are:
1. Do you feel that this sort of distribution is determined more by the instrument capabilities, or the inherent properties of the specimens observed? 2. If we were go for a ~5-10X $$$$ investment in a field emission scope a) would this distribution of magnifications change significantly and b) would the upgrade be demonstrably worthwhile?
I realize there is a big pile of ifs/ands/buts in these questions - I'm just looking for people who are willing to share thoughts, particularly if they have experience in taking this route.
Thanks as always,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
After twelve years of therapy my psychiatrist said something that brought tears to my eyes. He said, "No hablo ingles."
The enhanced capabilities of a FESEM, especially if it also has an in-lens detector and perhaps EDX and/or cryo capabilities, will not only do all that your present instruments does, but lots more. Thus the prospect of many new users who would find less satisfactory results with your present instrument. Larger user base means increased use and thus more revenue that hopefully will cover significantly increased service contract cost.
However, I would encourage holding on to your other scope if at all possible. It would make a great training scope and useful in its current role of lower magnification imaging.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
Jim, Thanks for the information. As I've had posted on my website for years, "Our experience has been that many SEMs are typically operated at or below 2,500X and rarely over 10,000X. If you don't require the very high resolution of field emission, the capabilities of an environmental SEM or the ability to remotely operate the system, your familiar and faithful SEM may still be your best friend."
Debbie has a point that the FESEM may open up new users, but x-ray still has volume limitations and low kV analysis still has limitations, particularly if you want quantitative results. My feeling is that biological, geological and failure analysis users are probably not going to be big users of the new capabilities. The big users will most likely be microelectronics, MEMS and nano-tube/particle studies. If these folks are in your system, they will love you.
If you can further parse your data, it would be interesting to see the breakdown of users at 10kx and up. Butterfly scales are fun, but how many micrographs of diatoms are in that range vs ICs and nanoparticles?
I'd love to hear from more people about this because generally it was only my IC customers who pushed mag all the time.
Our local ski area is claiming over 100" (254cm) so far for the season, but I think they get all their data from the summit. My yard is still looking like a good winter, though. I was never fond of those brown and gray winters that we generally had in south-central Pennsylvania. It's bright and beautiful outside my windows today.
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
Just my two cents-
Build it, and they will come. If you have a SFEG-SEM, then users will also take hi-mag images, when applicable.
Equally important, is having a range of detectors and high-voltages.
-JQuinn
I'm with Debby. Since we can get better than 1nm resolution and go up to 800,000x mag, we do. Our biologists are seeing things they've never seen before (look at the detail on this image from our S-4800 http://www.sciencemag.org/content/331/6016.cover-expansion ), and we have a lot more engineers coming in to look at thin films for photovoltaic cells, hydrogen fuel cells, etc. Sure, the diatom folks continue to use the lower mags, but I find them sneaking up to look at everything in better detail and perhaps finding new connections between those diatoms and other microorganisms. Hey, we can see bacteriophage erupting from cells in incredible detail. What's not to love? But, try to keep the old standby, too.
X-from Honolulu where there is absolutely no sign of snow and it's 78F, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
__________ Information from ESET NOD32 Antivirus, version of virus signature database 5924 (20110303) __________
The message was checked by ESET NOD32 Antivirus.
http://www.eset.com
==============================Original Headers============================== 52, 27 -- From eikonika-at-otenet.gr Fri Mar 4 02:52:53 2011 52, 27 -- Received: from echidna.otenet.gr (echidna.otenet.gr [83.235.69.17]) 52, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p248qqpS025168 52, 27 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 02:52:52 -0600 52, 27 -- Received: from kane.otenet.gr (kane.otenet.gr [83.235.67.31]) 52, 27 -- by echidna.otenet.gr (ESMTP) with ESMTP 52, 27 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 10:52:49 +0200 (EET) 52, 27 -- Received: from trump (athedsl-315552.home.otenet.gr [85.72.88.62]) 52, 27 -- (authenticated bits=0) 52, 27 -- by kane.otenet.gr (8.13.8/8.13.8/Debian-3) with ESMTP id p248qjVj004227 52, 27 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 10:52:48 +0200 52, 27 -- Message-ID: {E40481F11C06446995E1C25C9D193886-at-trump} 52, 27 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 52, 27 -- To: {microscopy-at-microscopy.com} 52, 27 -- Subject: Ultra high resolution in biology SEM: No, thanks 52, 27 -- Date: Fri, 4 Mar 2011 09:52:57 +0100 52, 27 -- MIME-Version: 1.0 52, 27 -- Content-Type: text/plain; 52, 27 -- format=flowed; 52, 27 -- charset="iso-8859-7"; 52, 27 -- reply-type=original 52, 27 -- Content-Transfer-Encoding: 7bit 52, 27 -- X-Priority: 3 52, 27 -- X-MSMail-Priority: Normal 52, 27 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931 52, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5931 52, 27 -- X-EsetScannerBuild: 8832 ==============================End of - Headers==============================
I've a student who's trying to do what amounts to dialysis on a support membrane. She has a protocol which requires her marine plankton samples to be dotted onto a section of membrane floating on buffer, the sea salt then allowed to dialyse through and the membrane floated down onto grids for analysis ( which will include EDX as well as plain TEM ). Her supervisor gave her a protocol using a nitrocellulose dissolved in a mixture of acetone and dichloroethane dropped onto water to make the membrane. With a lot of fiddling we found a solvent ratio that allows buffer exchange to occur but the resulting membrane is fragile under the beam and far from flat. Carbon coating strengthens it enough to be usable but it still looks like a bad micrograph of a sick sponge.
Has anyone tried anything similar and had better luck in producing a worthwhile coating? For various reasons they want to avoid pelleting and resuspending as a cleanup technique.
Cheers
Ian Portman
Imaging Suite Manager School of Life Sciences University of Warwick http://www2.warwick.ac.uk/fac/sci/lifesci/facilities/imaging
==============================Original Headers============================== 8, 36 -- From I.J.Portman-at-warwick.ac.uk Fri Mar 4 03:45:42 2011 8, 36 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8]) 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p249jf05010102 8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 03:45:42 -0600 8, 36 -- Received: from localhost (localhost [127.0.0.1]) 8, 36 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p249jfp4000215 8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:45:41 GMT 8, 36 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 8, 36 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1]) 8, 36 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 8, 36 -- with LMTP id hzqyD6aJstWS for {Microscopy-at-microscopy.com} ; 8, 36 -- Fri, 4 Mar 2011 09:45:41 +0000 (GMT) 8, 36 -- Received: from beech.ads.warwick.ac.uk (beech [137.205.97.163]) 8, 36 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p249jeOM000154 8, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:45:40 GMT 8, 36 -- X-Envelope-From: I.J.Portman-at-warwick.ac.uk 8, 36 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.181]) by beech.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 8, 36 -- Fri, 4 Mar 2011 09:45:39 +0000 8, 36 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 8, 36 -- Content-class: urn:content-classes:message 8, 36 -- MIME-Version: 1.0 8, 36 -- Content-Type: text/plain; 8, 36 -- charset="us-ascii" 8, 36 -- Subject: Semi Permeable EM support membranes 8, 36 -- Date: Fri, 4 Mar 2011 09:45:39 -0000 8, 36 -- Message-ID: {DB126BA68A8A5A439BAF072438A39FEA0294DB1C-at-ELDER.ads.warwick.ac.uk} 8, 36 -- X-MS-Has-Attach: 8, 36 -- X-MS-TNEF-Correlator: 8, 36 -- Thread-Topic: Semi Permeable EM support membranes 8, 36 -- Thread-Index: AcvaUOqfpHZHRZ/zSAeUCa2nRbHUZw== 8, 36 -- From: "Portman, Ian" {I.J.Portman-at-warwick.ac.uk} 8, 36 -- To: {Microscopy-at-microscopy.com} 8, 36 -- X-OriginalArrivalTime: 04 Mar 2011 09:45:39.0036 (UTC) FILETIME=[EB50A1C0:01CBDA50] 8, 36 -- X-DCC-Warwick-Metrics: nightshade; whitelist 8, 36 -- Content-Transfer-Encoding: 8bit 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p249jf05010102 ==============================End of - Headers==============================
Very nicely stated Yorgos. My experience in BioSEM is very limited, but I too was wondering about the "bumps" in the Science cover image, whether it could be metallic coating, an artifact or real.
On 3/4/11 2:53 AM, "eikonika-at-otenet.gr" {eikonika-at-otenet.gr} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Jim } } I read with interest your recent posting on magnifications people use in } your SEM lab, as well as the comments of other colleagues, and I would like } to add a few words: } To your first question if magnification is determined more by the instrument } capabilities or the inherent properties of the specimens observed, I would } say frankly: It's the specimens! } } I went through your site and saw your very decent micrographs that combine a } fine resolution with a nice depth of field. And saw that your Jeol } instrument has a 3-4 nm resolution limit that is not so far from Tina's } Hitachi with 1nm resolution. Do you think this can make a great difference } in your work? } } We all know that to reach these low numbers we need to have a perfectly } working source of electrons, a perfectly aligned scope, a perfectly attached } and coated specimen (like the ones for resolution assessment) and a perfect } focus and stigma etc. Obviously imperfections on these parameters will stop } us well before the ultimate resolution, and usually at around 10nm. } } Biology microscopists are like detectives, probably more than colleagues } from other fields of microscopy. We start from a live organism where the } cellular functions including plasma membrane morphology are changing every } little fraction of a second. We have to kill this organism as soon as } possible and fix it, then to take out the 70 or 80% of water and liquid from } around and inside it, and then coat it with metal (if we want to enjoy the } clear high resolution images of coated specimens as opposed to native state } cells viewed in low vacuum instruments). Then we take images and we try to } guess what the morphology can tell as in relation to the cell's function. } } To me sounds impossible that after all these torments the morphology of the } cell will stay intact at the level of 1 or 2nm that is really the size of } big biomolecules. And I think that if I could go so small in cell specimens } I would feel really embarrassed: For I would have to describe structures } appearing at this level that could be mostly artifacts. For instance the } cover on Science magazine that Tina showed us is a microbe with its surface } having thousands of tiny grains at the size of a few nanometers. Do these } grains represent any real structures or they are artifacts? And if the } micrograph is only for decoration, that's fine. But f it is part of the } research findings, it could be a trouble for the researcher to comment on } it. Nevertheless here I have to admit that my SEM experience is limited to } eukaryotic cells. } } It's amazing how we tend to get crazy with numbers and magnifications. It } happened to me several times to take a high mag picture on a biological } specimen (high for me is like 30K) only to find out afterwards that this } picture was not any better from a 5K one. For obtaining a nice clear } micrograph there are other parameters more important than the instrument's } ultimate resolution. Apart from those already mentioned it is also the } averaging and oversampling parameters -in other words. how much time the } electron beam scans the specimen. And of course the depth of field is } critical for the uneven surfaces found in biological samples. For me a real } improvement in biology SEM would be a substantial increase in the depth of } field, so that more of the specimen can be in focus. However, I have to } admit that my knowledge } } While writing these lines most of you guys from America are still sleeping, } so I hope this morning message will not spoil your mood. Have a nice day } from the ever shiny Athens. } } yorgos } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } eikonika-at-otenet.gr } www.aim.cat } *********************************** } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Listers: Good Day, eh? } } While fighting our current 119 cm (3.9 feet) of snow (and counting) my } mind began to wander and I became curious about the distribution of } magnification in the images we've acquired with our (tungsten-based) } SEM. Since we've logged all the images it wasn't hard to find out: } } http://www.mta.ca/dmf/sem_mag_dist.html } } We do SEM for a lot of different departments, but the bulk of our work } is biological, with heavy emphasis on microorganisms, particularly } diatoms. My questions to others out there who might have been in similar } circumstances are: } } 1. Do you feel that this sort of distribution is determined more by the } instrument capabilities, or the inherent properties of the specimens } observed? } 2. If we were go for a ~5-10X $$$$ investment in a field emission scope } a) would this distribution of magnifications change significantly and b) } would the upgrade be demonstrably worthwhile? } } I realize there is a big pile of ifs/ands/buts in these questions - I'm } just looking for people who are willing to share thoughts, particularly } if they have experience in taking this route. } } Thanks as always, } } Jim
==============================Original Headers============================== 6, 29 -- From RossLM-at-missouri.edu Fri Mar 4 05:42:31 2011 6, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24BgVZl028899 6, 29 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 05:42:31 -0600 6, 29 -- X-IronPort-Anti-Spam-Filtered: true 6, 29 -- X-IronPort-Anti-Spam-Result: AvsEAE9fcE2Dl3nN/2dsb2JhbACmY3S1WohrhWEEhRw 6, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 6, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 04 Mar 2011 05:42:31 -0600 6, 29 -- Received: from um-email05.um.umsystem.edu ([169.254.1.133]) by 6, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Fri, 4 Mar 2011 6, 29 -- 05:42:30 -0600 6, 29 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 6, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 29 -- Date: Fri, 4 Mar 2011 05:42:28 -0600 6, 29 -- Subject: Re: [Microscopy] Ultra high resolution in biology SEM: No, thanks 6, 29 -- Thread-Topic: [Microscopy] Ultra high resolution in biology SEM: No, thanks 6, 29 -- Thread-Index: AcvaSbMd1HqZdKa0R8qvOpUfbPQaVgAF4nfS 6, 29 -- Message-ID: {C9962C44.1AA80%rosslm-at-missouri.edu} 6, 29 -- In-Reply-To: {201103040853.p248rvb6026784-at-ns.microscopy.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 29 -- acceptlanguage: en-US 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24BgVZl028899 ==============================End of - Headers==============================
Also, an FESEM is MUCH easier to operate at high mag. You can wind most up to 50,000 times plus without even breaking a sweat!
I would like to shift the discussion to displaying horizontal field width and changing mag by steps of horizontal field width. Magnification has been meaningless for many years!
John Mansfield PhD Cphys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 11, 20 -- From jfmjfm-at-umich.edu Fri Mar 4 06:24:02 2011 11, 20 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.12.86]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24CO2PE013323 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 06:24:02 -0600 11, 20 -- Received: FROM [10.0.1.10] (c-68-40-121-17.hsd1.mi.comcast.net [68.40.121.17]) 11, 20 -- By tombraider.mr.itd.umich.edu ID 4D70D9E0.15066.16311 ; 11, 20 -- Authuser jfmjfm; 11, 20 -- 4 Mar 2011 07:24:00 EST 11, 20 -- Subject: Re: [Microscopy] mag distribution of SEM images 11, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 11, 20 -- In-Reply-To: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} 11, 20 -- Date: Fri, 4 Mar 2011 07:23:59 -0500 11, 20 -- Message-Id: {1DC6548B-6CFB-4492-BE39-BCD29E6E2B49-at-umich.edu} 11, 20 -- References: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- X-Mailer: Apple Mail (2.1082) 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24CO2PE013323 ==============================End of - Headers==============================
I agree with the statement referring to magnification. I find it a useful number to remember so that images are taken at an identical horizontal Field Width but only for that purpose. I always include in all reports the statement: "Note that magnifications are accurate when images are displayed at 30x26cm size. Micron bars are accurate at any display size."
Too many times I have people coming to me saying: "I want images with a 1µm bar." Now that is fine but that notation potentially represents a number of different magnifications. Thus it is nice to know accurate HFW or magnification from previous images so that future images can be captured at similar conditions.
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} } Reply-To: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} } Date: Fri, 4 Mar 2011 07:25:34 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Re: mag distribution of SEM images } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Also, an FESEM is MUCH easier to operate at high mag. } You can wind most up to 50,000 times plus without even breaking a sweat! } } I would like to shift the discussion to displaying horizontal field width and } changing mag by steps of horizontal field width. } Magnification has been meaningless for many years! } } } John Mansfield PhD Cphys MInstP } North Campus Electron Microbeam Analysis Laboratory } 417 SRB, University of Michigan } 2455 Hayward, Ann Arbor MI 48109-2143 USA } Phone: (734) 936-3352 FAX (734) 763-2282 } Cell. Phone: (734) 834-3913 } (Leaving a phone message at 936-3352 is preferable to 834-3913) } Email: jfmjfm-at-umich.edu } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html } Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° } 42.6439') } AIM: thejfmjfm } Yahoo: thejfmjfm } Skype: thejfmjfm } } Home address: } 4304 Spring Lake Boulevard } Ann Arbor MI 48108-9657 } Phone (734) 994-3096 } Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° } 45.7980') } } Please note: Electronic Mail is not secure, but should be read several times } every day, and should definitely be used for urgent or sensitive issues. } } } } } } } ==============================Original Headers============================== } 11, 20 -- From jfmjfm-at-umich.edu Fri Mar 4 06:24:02 2011 } 11, 20 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu } [141.211.12.86]) } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p24CO2PE013323 } 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 06:24:02 -0600 } 11, 20 -- Received: FROM [10.0.1.10] (c-68-40-121-17.hsd1.mi.comcast.net } [68.40.121.17]) } 11, 20 -- By tombraider.mr.itd.umich.edu ID 4D70D9E0.15066.16311 ; } 11, 20 -- Authuser jfmjfm; } 11, 20 -- 4 Mar 2011 07:24:00 EST } 11, 20 -- Subject: Re: [Microscopy] mag distribution of SEM images } 11, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) } 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 } 11, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} } 11, 20 -- In-Reply-To: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} } 11, 20 -- Date: Fri, 4 Mar 2011 07:23:59 -0500 } 11, 20 -- Message-Id: {1DC6548B-6CFB-4492-BE39-BCD29E6E2B49-at-umich.edu} } 11, 20 -- References: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} } 11, 20 -- To: microscopy-at-microscopy.com } 11, 20 -- X-Mailer: Apple Mail (2.1082) } 11, 20 -- Content-Transfer-Encoding: 8bit } 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p24CO2PE013323 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 31 -- From dsherman-at-purdue.edu Fri Mar 4 07:54:51 2011 8, 31 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24Dsp2W031536 8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 07:54:51 -0600 8, 31 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 8, 31 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p24DsoCe016346 8, 31 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 08:54:51 -0500 8, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB04H.purdue.lcl 8, 31 -- ([::1]) with mapi; Fri, 4 Mar 2011 08:54:50 -0500 8, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 8, 31 -- To: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} , 8, 31 -- "message to: MSA list" 8, 31 -- {microscopy-at-microscopy.com} 8, 31 -- Date: Fri, 4 Mar 2011 08:54:46 -0500 8, 31 -- Subject: Re: [Microscopy] Re: mag distribution of SEM images 8, 31 -- Thread-Topic: [Microscopy] Re: mag distribution of SEM images 8, 31 -- Thread-Index: AcvaZ0Nnnsri6mf6SnymXSiAUKbluAADHT9n 8, 31 -- Message-ID: {C9965956.C19C%dsherman-at-purdue.edu} 8, 31 -- In-Reply-To: {201103041225.p24CPY4Y016138-at-ns.microscopy.com} 8, 31 -- Accept-Language: en-US 8, 31 -- Content-Language: en-US 8, 31 -- X-MS-Has-Attach: 8, 31 -- X-MS-TNEF-Correlator: 8, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 8, 31 -- acceptlanguage: en-US 8, 31 -- Content-Type: text/plain; charset="iso-8859-1" 8, 31 -- MIME-Version: 1.0 8, 31 -- X-PMX-Version: 5.5.9.388399 8, 31 -- X-PerlMx-Virus-Scanned: Yes 8, 31 -- Content-Transfer-Encoding: 8bit 8, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24Dsp2W031536 ==============================End of - Headers==============================
Yes, it is just a simple software tweak. You could have the option of magnifications or HFW e.g. 200x 400x 800x etc. or something like 200µm, 100µm, 50µm, 25µm, etc. It's up to the manufacturers to implement it. Please guys!
-- John Mansfield PhD Cphys MInstP North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
On Mar 4, 2011, at 8:54 AM, Sherman, Debra M wrote:
} I agree with the statement referring to magnification. I find it a useful } number to remember so that images are taken at an identical horizontal Field } Width but only for that purpose. I always include in all reports the } statement: } "Note that magnifications are accurate when images are displayed at 30x26cm } size. Micron bars are accurate at any display size." } } Too many times I have people coming to me saying: "I want images with a 1µm } bar." Now that is fine but that notation potentially represents a number of } different magnifications. Thus it is nice to know accurate HFW or } magnification from previous images so that future images can be captured at } similar conditions. } } Debby } } -- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy/ } } } } From: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} } } Reply-To: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} } } Date: Fri, 4 Mar 2011 07:25:34 -0500 } } To: Debby Sherman {dsherman-at-purdue.edu} } } Subject: [Microscopy] Re: mag distribution of SEM images } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Also, an FESEM is MUCH easier to operate at high mag. } } You can wind most up to 50,000 times plus without even breaking a sweat! } } } } I would like to shift the discussion to displaying horizontal field width and } } changing mag by steps of horizontal field width. } } Magnification has been meaningless for many years! } } } } } } John Mansfield PhD Cphys MInstP } } North Campus Electron Microbeam Analysis Laboratory } } 417 SRB, University of Michigan } } 2455 Hayward, Ann Arbor MI 48109-2143 USA } } Phone: (734) 936-3352 FAX (734) 763-2282 } } Cell. Phone: (734) 834-3913 } } (Leaving a phone message at 936-3352 is preferable to 834-3913) } } Email: jfmjfm-at-umich.edu } } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html } } Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° } } 42.6439') } } AIM: thejfmjfm } } Yahoo: thejfmjfm } } Skype: thejfmjfm } } } } Home address: } } 4304 Spring Lake Boulevard } } Ann Arbor MI 48108-9657 } } Phone (734) 994-3096 } } Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° } } 45.7980') } } } } Please note: Electronic Mail is not secure, but should be read several times } } every day, and should definitely be used for urgent or sensitive issues. } } } } } } } } } } } } } } ==============================Original Headers============================== } } 11, 20 -- From jfmjfm-at-umich.edu Fri Mar 4 06:24:02 2011 } } 11, 20 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu } } [141.211.12.86]) } } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p24CO2PE013323 } } 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 06:24:02 -0600 } } 11, 20 -- Received: FROM [10.0.1.10] (c-68-40-121-17.hsd1.mi.comcast.net } } [68.40.121.17]) } } 11, 20 -- By tombraider.mr.itd.umich.edu ID 4D70D9E0.15066.16311 ; } } 11, 20 -- Authuser jfmjfm; } } 11, 20 -- 4 Mar 2011 07:24:00 EST } } 11, 20 -- Subject: Re: [Microscopy] mag distribution of SEM images } } 11, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) } } 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 } } 11, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} } } 11, 20 -- In-Reply-To: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} } } 11, 20 -- Date: Fri, 4 Mar 2011 07:23:59 -0500 } } 11, 20 -- Message-Id: {1DC6548B-6CFB-4492-BE39-BCD29E6E2B49-at-umich.edu} } } 11, 20 -- References: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} } } 11, 20 -- To: microscopy-at-microscopy.com } } 11, 20 -- X-Mailer: Apple Mail (2.1082) } } 11, 20 -- Content-Transfer-Encoding: 8bit } } 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id p24CO2PE013323 } } ==============================End of - Headers============================== } } }
==============================Original Headers============================== 11, 21 -- From jfmjfm-at-umich.edu Fri Mar 4 08:27:23 2011 11, 21 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.12.86]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24ERNdX015802 11, 21 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 08:27:23 -0600 11, 21 -- Received: FROM jfm-mac.engin.umich.edu (jfm-mac.engin.umich.edu [141.213.21.60]) 11, 21 -- By tombraider.mr.itd.umich.edu ID 4D70F6C8.5CCBC.16633 ; 11, 21 -- Authuser jfmjfm; 11, 21 -- 4 Mar 2011 09:27:20 EST 11, 21 -- Subject: Re: [Microscopy] Re: mag distribution of SEM images 11, 21 -- Mime-Version: 1.0 (Apple Message framework v1082) 11, 21 -- Content-Type: text/plain; charset=iso-8859-1 11, 21 -- From: John Mansfield {jfmjfm-at-umich.edu} 11, 21 -- In-Reply-To: {C9965956.C19C%dsherman-at-purdue.edu} 11, 21 -- Date: Fri, 4 Mar 2011 09:27:20 -0500 11, 21 -- Cc: "message to: MSA list" {microscopy-at-microscopy.com} 11, 21 -- Message-Id: {7921FA25-3383-46B6-B42B-D86D7198DD10-at-umich.edu} 11, 21 -- References: {C9965956.C19C%dsherman-at-purdue.edu} 11, 21 -- To: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 21 -- X-Mailer: Apple Mail (2.1082) 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24ERNdX015802 ==============================End of - Headers==============================
All of your points you make and those of others who have added to this discussion are well founded. Having spent almost 40 years with SEM and watched them develop, there are areas that I believe are even more important than the magnifications that we now achieve, they are the advances in relation to accelerating voltage and signal collection!
I trust I will see Lou at the upcoming Missouri course, where two FEG in lens detection systems will allow us to demonstrate that it is the ability to collect high level signals at low kV, this really takes SEM forward. Thus my answer to those buying a new SEM for other than "super light microscope applications" is to go for a FEGSEM. This advice is not just for "conventional" low kV abilities, little or no coating, but for the new ways of collecting signals.
To be able to look at an uncoated biological sample in your applications, using "in lens" backscatter, at say 1kV, is a revelation; at 100,000X if that is your choice! Add to this the ability to play with the signals in a FEGSEM. In these instruments you may obtain additional data through tuning the mix of pure secondary signals and converted backscatter, or balancing these signals to further reduce charge. All of these procedures become standard tools that will enhance our levels of understanding of our materials.
As a consultant I am often asked to make the clients "impossible" into possible and I have no doubt that my tasks have been made far easier though that magic combination of the field emission source and the through the lens secondary and backscatter techniques. Plus one additional point, the FEG provides a greater depth of field than the conventional tungsten hairpin or LaB6 source!
Regards
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: RossLM-at-missouri.edu [mailto:RossLM-at-missouri.edu] Sent: 04 March 2011 11:43 To: protrain-at-emcourses.com
Very nicely stated Yorgos. My experience in BioSEM is very limited, but I too was wondering about the "bumps" in the Science cover image, whether it could be metallic coating, an artifact or real.
On 3/4/11 2:53 AM, "eikonika-at-otenet.gr" {eikonika-at-otenet.gr} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Jim } } I read with interest your recent posting on magnifications people use in } your SEM lab, as well as the comments of other colleagues, and I would like } to add a few words: } To your first question if magnification is determined more by the instrument } capabilities or the inherent properties of the specimens observed, I would } say frankly: It's the specimens! } } I went through your site and saw your very decent micrographs that combine a } fine resolution with a nice depth of field. And saw that your Jeol } instrument has a 3-4 nm resolution limit that is not so far from Tina's } Hitachi with 1nm resolution. Do you think this can make a great difference } in your work? } } We all know that to reach these low numbers we need to have a perfectly } working source of electrons, a perfectly aligned scope, a perfectly attached } and coated specimen (like the ones for resolution assessment) and a perfect } focus and stigma etc. Obviously imperfections on these parameters will stop } us well before the ultimate resolution, and usually at around 10nm. } } Biology microscopists are like detectives, probably more than colleagues } from other fields of microscopy. We start from a live organism where the } cellular functions including plasma membrane morphology are changing every } little fraction of a second. We have to kill this organism as soon as } possible and fix it, then to take out the 70 or 80% of water and liquid from } around and inside it, and then coat it with metal (if we want to enjoy the } clear high resolution images of coated specimens as opposed to native state } cells viewed in low vacuum instruments). Then we take images and we try to } guess what the morphology can tell as in relation to the cell's function. } } To me sounds impossible that after all these torments the morphology of the } cell will stay intact at the level of 1 or 2nm that is really the size of } big biomolecules. And I think that if I could go so small in cell specimens } I would feel really embarrassed: For I would have to describe structures } appearing at this level that could be mostly artifacts. For instance the } cover on Science magazine that Tina showed us is a microbe with its surface } having thousands of tiny grains at the size of a few nanometers. Do these } grains represent any real structures or they are artifacts? And if the } micrograph is only for decoration, that's fine. But f it is part of the } research findings, it could be a trouble for the researcher to comment on } it. Nevertheless here I have to admit that my SEM experience is limited to } eukaryotic cells. } } It's amazing how we tend to get crazy with numbers and magnifications. It } happened to me several times to take a high mag picture on a biological } specimen (high for me is like 30K) only to find out afterwards that this } picture was not any better from a 5K one. For obtaining a nice clear } micrograph there are other parameters more important than the instrument's } ultimate resolution. Apart from those already mentioned it is also the } averaging and oversampling parameters -in other words. how much time the } electron beam scans the specimen. And of course the depth of field is } critical for the uneven surfaces found in biological samples. For me a real } improvement in biology SEM would be a substantial increase in the depth of } field, so that more of the specimen can be in focus. However, I have to } admit that my knowledge } } While writing these lines most of you guys from America are still sleeping, } so I hope this morning message will not spoil your mood. Have a nice day } from the ever shiny Athens. } } yorgos } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } eikonika-at-otenet.gr } www.aim.cat } *********************************** } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Listers: Good Day, eh? } } While fighting our current 119 cm (3.9 feet) of snow (and counting) my } mind began to wander and I became curious about the distribution of } magnification in the images we've acquired with our (tungsten-based) } SEM. Since we've logged all the images it wasn't hard to find out: } } http://www.mta.ca/dmf/sem_mag_dist.html } } We do SEM for a lot of different departments, but the bulk of our work } is biological, with heavy emphasis on microorganisms, particularly } diatoms. My questions to others out there who might have been in similar } circumstances are: } } 1. Do you feel that this sort of distribution is determined more by the } instrument capabilities, or the inherent properties of the specimens } observed? } 2. If we were go for a ~5-10X $$$$ investment in a field emission scope } a) would this distribution of magnifications change significantly and b) } would the upgrade be demonstrably worthwhile? } } I realize there is a big pile of ifs/ands/buts in these questions - I'm } just looking for people who are willing to share thoughts, particularly } if they have experience in taking this route. } } Thanks as always, } } Jim
==============================Original Headers============================== 6, 29 -- From RossLM-at-missouri.edu Fri Mar 4 05:42:31 2011 6, 29 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24BgVZl028899 6, 29 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 05:42:31 -0600 6, 29 -- X-IronPort-Anti-Spam-Filtered: true 6, 29 -- X-IronPort-Anti-Spam-Result: AvsEAE9fcE2Dl3nN/2dsb2JhbACmY3S1WohrhWEEhRw 6, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 6, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 04 Mar 2011 05:42:31 -0600 6, 29 -- Received: from um-email05.um.umsystem.edu ([169.254.1.133]) by 6, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Fri, 4 Mar 2011 6, 29 -- 05:42:30 -0600 6, 29 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 6, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 6, 29 -- Date: Fri, 4 Mar 2011 05:42:28 -0600 6, 29 -- Subject: Re: [Microscopy] Ultra high resolution in biology SEM: No, thanks 6, 29 -- Thread-Topic: [Microscopy] Ultra high resolution in biology SEM: No, thanks 6, 29 -- Thread-Index: AcvaSbMd1HqZdKa0R8qvOpUfbPQaVgAF4nfS 6, 29 -- Message-ID: {C9962C44.1AA80%rosslm-at-missouri.edu} 6, 29 -- In-Reply-To: {201103040853.p248rvb6026784-at-ns.microscopy.com} 6, 29 -- Accept-Language: en-US 6, 29 -- Content-Language: en-US 6, 29 -- X-MS-Has-Attach: 6, 29 -- X-MS-TNEF-Correlator: 6, 29 -- user-agent: Microsoft-Entourage/13.8.0.101117 6, 29 -- acceptlanguage: en-US 6, 29 -- Content-Type: text/plain; charset="us-ascii" 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24BgVZl028899 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 27 -- From protrain-at-emcourses.com Fri Mar 4 08:48:49 2011 17, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24EmlhT032222 17, 27 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 08:48:48 -0600 17, 27 -- Received: from 5ac8bfa2.bb.sky.com ([90.200.191.162]:52874 helo=ProtrainPC) 17, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 17, 27 -- (envelope-from {protrain-at-emcourses.com} ) 17, 27 -- id 1PvWJA-0004Fa-KP by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 04 Mar 2011 16:48:45 +0200 17, 27 -- Reply-To: {protrain-at-emcourses.com} 17, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 17, 27 -- To: {RossLM-at-missouri.edu} 17, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 17, 27 -- References: {201103041143.p24BhLQj030141-at-ns.microscopy.com} 17, 27 -- In-Reply-To: {201103041143.p24BhLQj030141-at-ns.microscopy.com} 17, 27 -- Subject: RE: [Microscopy] Re: Ultra high resolution in biology SEM: No, thanks 17, 27 -- Date: Fri, 4 Mar 2011 14:48:18 -0000 17, 27 -- Organization: Protrain 17, 27 -- Message-ID: {002301cbda7b$41c2f740$c548e5c0$-at-com} 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Type: text/plain; 17, 27 -- charset="us-ascii" 17, 27 -- Content-Transfer-Encoding: 7bit 17, 27 -- X-Mailer: Microsoft Office Outlook 12.0 17, 27 -- Thread-Index: AcvaYWpIVD6jOADITQyMXIQiUnfqVgAE/lyA 17, 27 -- Content-Language: en-gb 17, 27 -- X-Scan-Signature: fcd005d100313b0d5223193951c0d55c{237}} 17, 27 -- X-Trace: smtp01.dial-up.net 1PvWJA-0004Fa-KP fc35320aa71c2437db2edfaa19a57a38 ==============================End of - Headers==============================
First, thanks for all the informative replies. Their variety, though, tells me this is what I get for asking imprecise questions! Let me rephrase my curiosity on this subject with two more observations/questions. Please take my numbers as assumed - I know there is wiggle room in all the values I give, but that's not where my questions are directed.
1. With a (vendor stated) resolution of 3.5 nm, and assuming the human eye has a resolution of 0.1 mm (I wish I still did), the "useful" magnification (or associated horizontal field width) of our W-based SEM is around 30,000X. My bar chart shows that the mode of the magnification used is 10,000X (mean is about 8,000X). So we appear to be "underutilizing" the instrument by a factor of at least 3 quite a bit of the time. So taking the same assumptions for a FE-based instrument with 1 nm resolution, useful magnification is 100,000X. If a similar bar chart could be constructed for this instrument, would the mode/mean used be in 30,000X region? Or would the distribution of mags be much more seriously skewed toward higher magnification images?
2. Is the 5-10X increase in cost (and presumably maintenance) worth it, considering that 30,000X images on the W-based instrument are certainly possible (and passable)? Or will the magnification distribution be so significantly skewed toward the higher mags that the W-based instrument is left entirely in the dust?
I supposed my ulterior motive in asking these questions stems from the fact that if we could actually secure the funding for a FE-SEM, we almost certainly could not afford to keep them both. Are the current plow-horse users going to be so enchanted with the new Lamborghini that they'll be willing to support the instrument with more use and/or higher fees? If they walk away, will new users step forward to take advantage of the enhanced capability? No one can answer that for me in my particular situation, but I'm interested in comments from others in similar circumstances.
Thanks in advance,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Having HFW in the data bar on FEI SEMs is a choice you can make in preferences. I keep magnification, micron bark and HFW in the data bar for convenience. I do not know about TEMs. -- Debby
} From: John Mansfield {jfmjfm-at-umich.edu} } Date: Fri, 4 Mar 2011 09:27:20 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Cc: "message to: MSA list" {microscopy-at-microscopy.com} } Subject: Re: [Microscopy] Re: mag distribution of SEM images } } Yes, it is just a simple software tweak. You could have the option of } magnifications or HFW } e.g. } 200x 400x 800x etc. } or something like 200µm, 100µm, 50µm, 25µm, etc. } It's up to the manufacturers to implement it. } Please guys! } } -- } John Mansfield PhD Cphys MInstP } North Campus Electron Microbeam Analysis Laboratory } 417 SRB, University of Michigan } 2455 Hayward, Ann Arbor MI 48109-2143 USA } Phone: (734) 936-3352 FAX (734) 763-2282 } Cell. Phone: (734) 834-3913 } (Leaving a phone message at 936-3352 is preferable to 834-3913) } Email: jfmjfm-at-umich.edu } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html } Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° } 42.6439') } AIM: thejfmjfm } Yahoo: thejfmjfm } Skype: thejfmjfm } } Home address: } 4304 Spring Lake Boulevard } Ann Arbor MI 48108-9657 } Phone (734) 994-3096 } Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° } 45.7980') } } Please note: Electronic Mail is not secure, but should be read several times } every day, and should definitely be used for urgent or sensitive issues. } } } } } On Mar 4, 2011, at 8:54 AM, Sherman, Debra M wrote: } } } I agree with the statement referring to magnification. I find it a useful } } number to remember so that images are taken at an identical horizontal Field } } Width but only for that purpose. I always include in all reports the } } statement: } } "Note that magnifications are accurate when images are displayed at 30x26cm } } size. Micron bars are accurate at any display size." } } } } Too many times I have people coming to me saying: "I want images with a 1µm } } bar." Now that is fine but that notation potentially represents a number of } } different magnifications. Thus it is nice to know accurate HFW or } } magnification from previous images so that future images can be captured at } } similar conditions. } } } } Debby } } } } -- } } Debby Sherman, Director Phone: 765-494-6666 } } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail: dsherman-at-purdue.edu } } S-052 Whistler Building } } 170 S. University Street } } West Lafayette, IN 47907 } } http://www.ag.purdue.edu/facilities/microscopy/ } } } } } } } From: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} } } } Reply-To: "jfmjfm-at-umich.edu" {jfmjfm-at-umich.edu} } } } Date: Fri, 4 Mar 2011 07:25:34 -0500 } } } To: Debby Sherman {dsherman-at-purdue.edu} } } } Subject: [Microscopy] Re: mag distribution of SEM images } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Also, an FESEM is MUCH easier to operate at high mag. } } } You can wind most up to 50,000 times plus without even breaking a sweat! } } } } } } I would like to shift the discussion to displaying horizontal field width } } } and } } } changing mag by steps of horizontal field width. } } } Magnification has been meaningless for many years! } } } } } } } } } John Mansfield PhD Cphys MInstP } } } North Campus Electron Microbeam Analysis Laboratory } } } 417 SRB, University of Michigan } } } 2455 Hayward, Ann Arbor MI 48109-2143 USA } } } Phone: (734) 936-3352 FAX (734) 763-2282 } } } Cell. Phone: (734) 834-3913 } } } (Leaving a phone message at 936-3352 is preferable to 834-3913) } } } Email: jfmjfm-at-umich.edu } } } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html } } } Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° } } } 42.6439') } } } AIM: thejfmjfm } } } Yahoo: thejfmjfm } } } Skype: thejfmjfm } } } } } } Home address: } } } 4304 Spring Lake Boulevard } } } Ann Arbor MI 48108-9657 } } } Phone (734) 994-3096 } } } Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 } } } ° } } } 45.7980') } } } } } } Please note: Electronic Mail is not secure, but should be read several times } } } every day, and should definitely be used for urgent or sensitive issues. } } } } } } } } } } } } } } } } } } } } } ==============================Original Headers============================== } } } 11, 20 -- From jfmjfm-at-umich.edu Fri Mar 4 06:24:02 2011 } } } 11, 20 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu } } } [141.211.12.86]) } } } 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } p24CO2PE013323 } } } 11, 20 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 06:24:02 -0600 } } } 11, 20 -- Received: FROM [10.0.1.10] (c-68-40-121-17.hsd1.mi.comcast.net } } } [68.40.121.17]) } } } 11, 20 -- By tombraider.mr.itd.umich.edu ID 4D70D9E0.15066.16311 ; } } } 11, 20 -- Authuser jfmjfm; } } } 11, 20 -- 4 Mar 2011 07:24:00 EST } } } 11, 20 -- Subject: Re: [Microscopy] mag distribution of SEM images } } } 11, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) } } } 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 } } } 11, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} } } } 11, 20 -- In-Reply-To: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} } } } 11, 20 -- Date: Fri, 4 Mar 2011 07:23:59 -0500 } } } 11, 20 -- Message-Id: {1DC6548B-6CFB-4492-BE39-BCD29E6E2B49-at-umich.edu} } } } 11, 20 -- References: {201103031902.p23J2MaQ021471-at-ns.microscopy.com} } } } 11, 20 -- To: microscopy-at-microscopy.com } } } 11, 20 -- X-Mailer: Apple Mail (2.1082) } } } 11, 20 -- Content-Transfer-Encoding: 8bit } } } 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } } ns.microscopy.com id p24CO2PE013323 } } } ==============================End of - Headers============================== } } } } } } }
==============================Original Headers============================== 5, 30 -- From dsherman-at-purdue.edu Fri Mar 4 09:18:03 2011 5, 30 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24FI3cv032261 5, 30 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:18:03 -0600 5, 30 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 5, 30 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p24FI3n5007720 5, 30 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 10:18:03 -0500 5, 30 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB01F.purdue.lcl 5, 30 -- ([::1]) with mapi; Fri, 4 Mar 2011 10:18:02 -0500 5, 30 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 5, 30 -- To: John Mansfield {jfmjfm-at-umich.edu} 5, 30 -- CC: "message to: MSA list" {microscopy-at-microscopy.com} 5, 30 -- Date: Fri, 4 Mar 2011 10:18:00 -0500 5, 30 -- Subject: Re: [Microscopy] Re: mag distribution of SEM images 5, 30 -- Thread-Topic: [Microscopy] Re: mag distribution of SEM images 5, 30 -- Thread-Index: AcvaeEea/a8n7xRwRXqZQSJoJNOhCwABxF3d 5, 30 -- Message-ID: {C9966CD8.C1B3%dsherman-at-purdue.edu} 5, 30 -- In-Reply-To: {7921FA25-3383-46B6-B42B-D86D7198DD10-at-umich.edu} 5, 30 -- Accept-Language: en-US 5, 30 -- Content-Language: en-US 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- user-agent: Microsoft-Entourage/13.8.0.101117 5, 30 -- acceptlanguage: en-US 5, 30 -- Content-Type: text/plain; charset="iso-8859-1" 5, 30 -- MIME-Version: 1.0 5, 30 -- X-PMX-Version: 5.5.9.388399 5, 30 -- X-PerlMx-Virus-Scanned: Yes 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24FI3cv032261 ==============================End of - Headers==============================
Our use probably increased at least 10x within the first year of when we got the first FESEM compared to the previous year using the older (but still working to spec) W-SEM. It continues to maintain that higher usage 5 years later. The ease of use and the possibilities opened up what had been virtually entirely biological samples to the chemists, pharmacists, and engineers while still satisfying the biologists. Some users disappear but new ones take their place. The difference in a 20K image from the FESEM to that of a W-instrument is startling even without the in-lens detector but double so with the in-lens. Combinations of low kV and low vacuum options on many modern instruments open up lots of new possibilities.
I totally understand your concern about higher cost-of-ownership. That has indeed been a factor in our decisions. However, this has to be balanced with new capabilities if the research is going to move forward. You must make your administration aware of this and get their commitment to help out initially if necessary. The year guarantee that manufacturers give on new instruments is usually sufficient to build your user base. We started charging immediately so that at the end of the first year we had the money in the bank for the service contract. Thus we are not "in the red" at the beginning of each fiscal year. We have timed our contracts so they are due at the beginning of the fiscal year so can easily justify any carryover from the previous year as it is already committed.
When looking for a new instruments (or contemplating this) always think about the future not the present and the versatility that can be gained from a particular instrument.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "jehrman-at-mta.ca" {jehrman-at-mta.ca} } Reply-To: "jehrman-at-mta.ca" {jehrman-at-mta.ca} } Date: Fri, 4 Mar 2011 09:57:33 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] Thanks: mag distribution of images } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } First, thanks for all the informative replies. Their variety, though, } tells me this is what I get for asking imprecise questions! Let me } rephrase my curiosity on this subject with two more } observations/questions. Please take my numbers as assumed - I know there } is wiggle room in all the values I give, but that's not where my } questions are directed. } } 1. With a (vendor stated) resolution of 3.5 nm, and assuming the human } eye has a resolution of 0.1 mm (I wish I still did), the "useful" } magnification (or associated horizontal field width) of our W-based SEM } is around 30,000X. My bar chart shows that the mode of the magnification } used is 10,000X (mean is about 8,000X). So we appear to be } "underutilizing" the instrument by a factor of at least 3 quite a bit of } the time. So taking the same assumptions for a FE-based instrument with } 1 nm resolution, useful magnification is 100,000X. If a similar bar } chart could be constructed for this instrument, would the mode/mean used } be in 30,000X region? Or would the distribution of mags be much more } seriously skewed toward higher magnification images? } } 2. Is the 5-10X increase in cost (and presumably maintenance) worth it, } considering that 30,000X images on the W-based instrument are certainly } possible (and passable)? Or will the magnification distribution be so } significantly skewed toward the higher mags that the W-based instrument } is left entirely in the dust? } } I supposed my ulterior motive in asking these questions stems from the } fact that if we could actually secure the funding for a FE-SEM, we } almost certainly could not afford to keep them both. Are the current } plow-horse users going to be so enchanted with the new Lamborghini that } they'll be willing to support the instrument with more use and/or higher } fees? If they walk away, will new users step forward to take advantage } of the enhanced capability? No one can answer that for me in my } particular situation, but I'm interested in comments from others in } similar circumstances. } } Thanks in advance, } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } A much wittier reply came to mind } immediately after I clicked the 'Send' button. } } } ==============================Original Headers============================== } 12, 19 -- From jehrman-at-mta.ca Fri Mar 4 08:56:01 2011 } 12, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.114]) } 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p24Eu1tU011886 } 12, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 08:56:01 -0600 } 12, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:56270) } 12, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) } 12, 19 -- (Exim 4.71) } 12, 19 -- (envelope-from {jehrman-at-mta.ca} ) } 12, 19 -- id 1PvWQC-00076u-Pn } 12, 19 -- for Microscopy-at-microscopy.com; Fri, 04 Mar 2011 10:56:01 -0400 } 12, 19 -- Message-ID: {4D70FD7D.6010300-at-mta.ca} } 12, 19 -- Date: Fri, 04 Mar 2011 10:55:57 -0400 } 12, 19 -- From: James Ehrman {jehrman-at-mta.ca} } 12, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; } rv:1.9.2.14) Gecko/20110221 Thunderbird/3.1.8 } 12, 19 -- MIME-Version: 1.0 } 12, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} } 12, 19 -- Subject: Thanks: mag distribution of images } 12, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 19 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 31 -- From dsherman-at-purdue.edu Fri Mar 4 09:34:34 2011 9, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24FYYC2016139 9, 31 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:34:34 -0600 9, 31 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 9, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p24FYXP6017305; 9, 31 -- Fri, 4 Mar 2011 10:34:34 -0500 9, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB02F.purdue.lcl 9, 31 -- ([::1]) with mapi; Fri, 4 Mar 2011 10:34:33 -0500 9, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 9, 31 -- To: "jehrman-at-mta.ca" {jehrman-at-mta.ca} , 9, 31 -- "message to: MSA list" 9, 31 -- {microscopy-at-microscopy.com} 9, 31 -- Date: Fri, 4 Mar 2011 10:34:31 -0500 9, 31 -- Subject: Re: [Microscopy] Thanks: mag distribution of images 9, 31 -- Thread-Topic: [Microscopy] Thanks: mag distribution of images 9, 31 -- Thread-Index: AcvafH7jyeAoF/TPQK+AyyqTo5TXJwABSjYf 9, 31 -- Message-ID: {C99670B7.C1B7%dsherman-at-purdue.edu} 9, 31 -- In-Reply-To: {201103041457.p24EvX6g017033-at-ns.microscopy.com} 9, 31 -- Accept-Language: en-US 9, 31 -- Content-Language: en-US 9, 31 -- X-MS-Has-Attach: 9, 31 -- X-MS-TNEF-Correlator: 9, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 9, 31 -- acceptlanguage: en-US 9, 31 -- Content-Type: text/plain; charset="us-ascii" 9, 31 -- MIME-Version: 1.0 9, 31 -- X-PMX-Version: 5.5.9.388399 9, 31 -- X-PerlMx-Virus-Scanned: Yes 9, 31 -- Content-Transfer-Encoding: 8bit 9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24FYYC2016139 ==============================End of - Headers==============================
The thread on facilities and their misuse by users has been longer than most threads and has been full of wisdom: train all users; restrict those who train; have users take formal courses (where available); give check lists or have users make their own check sheets; the two-finger rule; test users; and more. It seems to me that it would be well worth while for someone to go through all the contributions and digest them into a composite list: a check list for facility directors, if you will. Of course there may be differences of opinion about what should be on the list.
For example, despite the guidance from such a list, damage to the instruments can and will happen. How much should the operation of the facility be designed to create an environment in which the user who causes damage feels free to report it, and how much should the environment be such as to discourage damage in the first place? Charging users for damage may discourage errors, but does not encourage reporting them after the event.
However, when I wrote my original contribution I had something else in mind. In speaking to facility directors over the years I have recognized two distinct philosophies.
Philosophy 1. The instruments belong to the facility. Users are guests. Operation of the instruments should be limited to methods described by the manufacturers manual, the recommendations of the maintenance engineer, and the instructions of the facility manager.
Philosophy 2. The instruments are a resource. During the time that they are using it, the user should feel that they can treat the instrument as if it were their own. Users should not be discouraged from trying to operate the instrument in ways that have not previously been tried (though they should be encouraged or required to talk to facility staff before doing so).
I think that the first point of view is the majority view, but I have always believed strongly in the second (even though it leads to a few more occasions on which instruments get damaged). I believe that it leads to users who develop a deeper understanding of the instruments they use, and that it leads to better and more original research.
Alwyn
-- Alwyn Eades Lehigh University
==============================Original Headers============================== 10, 21 -- From jae5-at-lehigh.edu Fri Mar 4 09:48:53 2011 10, 21 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 10, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24FmqSM032351 10, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:48:53 -0600 10, 21 -- Received: from [192.168.0.199] (70.15.4.31.res-cmts.sm.ptd.net [70.15.4.31]) 10, 21 -- (authenticated bits=0) 10, 21 -- by rain.cc.lehigh.edu (8.14.4/8.14.4) with ESMTP id p24FmjqY018139 10, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 10, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 10:48:52 -0500 10, 21 -- Message-ID: {4D7109E0.3070107-at-lehigh.edu} 10, 21 -- Date: Fri, 04 Mar 2011 10:48:48 -0500 10, 21 -- From: Alwyn Eades {jae5-at-lehigh.edu} 10, 21 -- Reply-To: jae5-at-lehigh.edu 10, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.14) Gecko/20110221 Thunderbird/3.1.8 10, 21 -- MIME-Version: 1.0 10, 21 -- To: Microscopy-at-microscopy.com 10, 21 -- Subject: Facility operation 10, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 21 -- Content-Transfer-Encoding: 7bit 10, 21 -- X-Virus-Scanned: clamav-milter 0.97 at rain.cc.lehigh.edu 10, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
We are trying to do quant on a multi-layer thin film on Molybdenum substrate. In order to get the different layers we are varying the accelerating voltage (7, 10, 20, & 25kV). EDX unit is an SDD with 80mm window so counts are basically good at all kVs (1KC/S or greater). Spectra were collected for up to 500sec.
Layers in order are: Top: Indium oxide (~250nm) middle: Zinc Oxide (~50nm) middle 2: Cadium Sulfide (~30nm) Bottom: Copper/Indium/Gallium/Sulfur/Selinium (~750nm) Substrate: Mo (800 nm)
At 7kV, we get S and Se at rations of ~ 50:50. Expected was 20:80. This ratio holds for 10 or 20kV as well. At 25kV Mo is detected.
The ratio is expected to be 20:80 in the CuInGaSSe layer based on the lattice constants (determined by XRD) being slightly smaller than that of the pure selenide phase. Using Vegard's Law, the S:Se ratio of 20:80 is obtained. Previous EDX measurements using a SiLi detector, with the only layer being the CuInGaSSe layer, also showed a S:Se ratio of roughly 20:80.
Any explanations for the ratio differences would be appreciated. Suggestions for improving accuracy would be appreciated as would comments related to dealing with EDX of Sulfur.
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 10, 28 -- From dsherman-at-purdue.edu Fri Mar 4 12:44:43 2011 10, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24Iihvg020936 10, 28 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 12:44:43 -0600 10, 28 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) 10, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p24IihQn001798 10, 28 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 13:44:43 -0500 10, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB03H.purdue.lcl 10, 28 -- ([172.21.6.92]) with mapi; Fri, 4 Mar 2011 13:44:42 -0500 10, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 28 -- Date: Fri, 4 Mar 2011 13:44:39 -0500 10, 28 -- Subject: EDX on Sulfur 10, 28 -- Thread-Topic: EDX on Sulfur 10, 28 -- Thread-Index: AcvanDdv/st73CcoPkGs2jNdGhwr8Q== 10, 28 -- Message-ID: {C9969D47.C1CD%dsherman-at-purdue.edu} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- X-PMX-Version: 5.5.9.388399 10, 28 -- X-PerlMx-Virus-Scanned: Yes 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24Iihvg020936 ==============================End of - Headers==============================
Our experience has been similar to Debby Sherman's; we have enough users to support the instrument and we appeal to people in all kinds of fields (although we do not have some of the enhanced capabiities Debby has, such as cryo). I find training people on the FESEM a joy, and each user is trained to use the instrument just about to its maximum capabilities, even if they aren't going to go there. And most of them really get it.
Read Steve Chapman's post; it is the newer capabilities of these FESEMs that really makes the difference. Yes, when we started seeing rough surfaces on microbes that formerly looked smooth to us, we turned ourselves inside out making sure this was not any kind of artifact. After two ears with this instrument, we are *very* confident in what we are seeing (yes, as far as you can be with chemical fixation, not ultrarapid cryo). With low kV and high signal detection, we are seeing surface structures that were formerly obscured by signals generated below the surface. The difference is astounding. Also explainable and reproducible. Trust me on this, or come and visit.
Magnification? When I train users, I do make the point that "magnification" has become meaningless, but I still encourage them to leave both the scale bar (which gives a false sense that you can actually measure something) and the mag on the image data bar, because that at least tells me something about the conditions under which that image was taken (as well as accelerating voltage and working distance).
Aloha, Tina
} Our use probably increased at least 10x within the first year of when we got } the first FESEM compared to the previous year using the older (but still } working to spec) W-SEM. It continues to maintain that higher usage 5 years } later. The ease of use and the possibilities opened up what had been } virtually entirely biological samples to the chemists, pharmacists, and } engineers while still satisfying the biologists. Some users disappear but } new ones take their place. The difference in a 20K image from the FESEM to } that of a W-instrument is startling even without the in-lens detector but } double so with the in-lens. Combinations of low kV and low vacuum options } on many modern instruments open up lots of new possibilities. } } I totally understand your concern about higher cost-of-ownership. That has } indeed been a factor in our decisions. However, this has to be balanced } with new capabilities if the research is going to move forward. You must } make your administration aware of this and get their commitment to help out } initially if necessary. The year guarantee that manufacturers give on new } instruments is usually sufficient to build your user base. We started } charging immediately so that at the end of the first year we had the money } in the bank for the service contract. Thus we are not "in the red" at the } beginning of each fiscal year. We have timed our contracts so they are due } at the beginning of the fiscal year so can easily justify any carryover from } the previous year as it is already committed. } } When looking for a new instruments (or contemplating this) always think } about the future not the present and the versatility that can be gained from } a particular instrument. } } Debby
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 6, 22 -- From tina-at-pbrc.hawaii.edu Fri Mar 4 13:00:09 2011 6, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24J09Cl004760 6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 13:00:09 -0600 6, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 6, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p24J08AK023276 6, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:00:08 -1000 (HST) 6, 22 -- Received: from localhost (tina-at-localhost) 6, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p24J07XB023272 6, 22 -- for {Microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 09:00:08 -1000 (HST) 6, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 6, 22 -- Date: Fri, 4 Mar 2011 09:00:07 -1000 (HST) 6, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 6, 22 -- X-X-Sender: tina-at-b1000 6, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 6, 22 -- Subject: Re: [Microscopy] Re: Thanks: mag distribution of images 6, 22 -- In-Reply-To: {201103041535.p24FZDn9017445-at-ns.microscopy.com} 6, 22 -- Message-ID: {Pine.GSO.4.64.1103040843420.22901-at-b1000} 6, 22 -- References: {201103041535.p24FZDn9017445-at-ns.microscopy.com} 6, 22 -- MIME-Version: 1.0 6, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Debby, Couple of things that you will have problems with using EDS on CIGS: 1) Se is volatile and moves under e-beam in the sample; 2) the additional issue is Mo-L alpa1 peak in substrate that directly overlaps with S-K in EDS; the Mo-K peaks are at 18-20KeV and need the higher acceleration voltage to be excited. But even at 7KV, you are generating Mo-Ls and your interaction volume is still well over 1um in diameter so there is plenty of Mo x-rays generated from the substrate and by fluorescence.
We find that TEM based EDS analysis is closer to what we measure in bulk concentration by chemical methods combined with mass spectroscopy and Neutron Activation Analysis. If you don't need to be using micro and nano-resolution, you would be better off using bulk techniques. The only technique that will give you accurate whole concentration of all elements in the bulk is NAA as chemical dissolution has issues with various phases in the complicated film stack.
Hope this helps,
Jerzy
*************************************************** Jerzy Gazda Ph.D. Section Manager - TEM, FIB, SEM Cerium Laboratories LLC 5204 E. Ben White Blvd. MS-512 Austin, TX 78741
(512) 934-5185 vm (512) 622-6600 pgr
www.ceriumlabs.com
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Friday, March 04, 2011 12:53 PM To: Gazda, Jerzy
Listers,
We are trying to do quant on a multi-layer thin film on Molybdenum substrate. In order to get the different layers we are varying the accelerating voltage (7, 10, 20, & 25kV). EDX unit is an SDD with 80mm window so counts are basically good at all kVs (1KC/S or greater). Spectra were collected for up to 500sec.
Layers in order are: Top: Indium oxide (~250nm) middle: Zinc Oxide (~50nm) middle 2: Cadium Sulfide (~30nm) Bottom: Copper/Indium/Gallium/Sulfur/Selinium (~750nm) Substrate: Mo (800 nm)
At 7kV, we get S and Se at rations of ~ 50:50. Expected was 20:80. This ratio holds for 10 or 20kV as well. At 25kV Mo is detected.
The ratio is expected to be 20:80 in the CuInGaSSe layer based on the lattice constants (determined by XRD) being slightly smaller than that of the pure selenide phase. Using Vegard's Law, the S:Se ratio of 20:80 is obtained. Previous EDX measurements using a SiLi detector, with the only layer being the CuInGaSSe layer, also showed a S:Se ratio of roughly 20:80.
Any explanations for the ratio differences would be appreciated. Suggestions for improving accuracy would be appreciated as would comments related to dealing with EDX of Sulfur.
Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 10, 28 -- From dsherman-at-purdue.edu Fri Mar 4 12:44:43 2011 10, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24Iihvg020936 10, 28 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 12:44:43 -0600 10, 28 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) 10, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p24IihQn001798 10, 28 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 13:44:43 -0500 10, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB03H.purdue.lcl 10, 28 -- ([172.21.6.92]) with mapi; Fri, 4 Mar 2011 13:44:42 -0500 10, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 28 -- Date: Fri, 4 Mar 2011 13:44:39 -0500 10, 28 -- Subject: EDX on Sulfur 10, 28 -- Thread-Topic: EDX on Sulfur 10, 28 -- Thread-Index: AcvanDdv/st73CcoPkGs2jNdGhwr8Q== 10, 28 -- Message-ID: {C9969D47.C1CD%dsherman-at-purdue.edu} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- X-PMX-Version: 5.5.9.388399 10, 28 -- X-PerlMx-Virus-Scanned: Yes 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24Iihvg020936 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 29 -- From Jerzy.Gazda-at-ceriumlabs.com Fri Mar 4 14:20:32 2011 22, 29 -- Received: from USSVLMGTY02.spansion.com (ussvlmgty02.spansion.com [12.151.36.113]) 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24KKWWO022301 22, 29 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 14:20:32 -0600 22, 29 -- Received: from ussvlvexcasht2.spansion.com ([10.247.35.124]) 22, 29 -- by USSVLMGTY02.spansion.com with ESMTP; 04 Mar 2011 12:20:31 -0800 22, 29 -- Received: from US2K7EXMBPF1.spansion.com ([10.247.27.4]) by 22, 29 -- USSVLVEXCASHT2.spansion.com ([10.247.35.124]) with mapi; Fri, 4 Mar 2011 22, 29 -- 12:20:31 -0800 22, 29 -- From: "Gazda, Jerzy" {Jerzy.Gazda-at-ceriumlabs.com} 22, 29 -- To: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} , 22, 29 -- "microscopy-at-microscopy.com" 22, 29 -- {microscopy-at-microscopy.com} 22, 29 -- Date: Fri, 4 Mar 2011 12:20:29 -0800 22, 29 -- Subject: RE: [Microscopy] EDX on Sulfur 22, 29 -- Thread-Topic: [Microscopy] EDX on Sulfur 22, 29 -- Thread-Index: AcvanWV1m4/MjSDxTQqfztBSMZl7bAACiErA 22, 29 -- Message-ID: {4638FCC8C3C4D543B4C53024563E5C4C8F156688-at-US2K7EXMBPF1.spansion.com} 22, 29 -- References: {201103041852.p24Iqll2001032-at-ns.microscopy.com} 22, 29 -- In-Reply-To: {201103041852.p24Iqll2001032-at-ns.microscopy.com} 22, 29 -- Accept-Language: en-US 22, 29 -- Content-Language: en-US 22, 29 -- X-MS-Has-Attach: 22, 29 -- X-MS-TNEF-Correlator: 22, 29 -- acceptlanguage: en-US 22, 29 -- Content-Type: text/plain; charset="us-ascii" 22, 29 -- MIME-Version: 1.0 22, 29 -- Content-Transfer-Encoding: 8bit 22, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24KKWWO022301 ==============================End of - Headers==============================
Jim, As a modestly heavy user of Biological FESEM, in my opinion, it is not about the magnification. Other features are more important.
1. Ease of use. Biological samples are really variable. It's not finding one picture and then going away. I have to sample extensively. With a modern FESEM, I might get 100 images in a few hour session. The alignment, stigmation and so on are really stable and simple to adjust, even when chaging kv or other operating parameters.
2. Low mag - high mag. In biological samples, we often have a large sample with dispersed small features of interest. We need good *ultra* low mag for searching and high mag (not necessarily extreme hi mag) for imaging, and easy switching between them. This occurs with modern instruments.
3. Low voltage. I do my imaging at 1 to 3 kV. In that way, I am looking at features at or near the surface, which is what I want to see. With the typical 15 kV, the features I need to image would be smeared out into the deep interaction volume. The low voltage regime opens up huge possibilities. The magnifications might not change that much but the sample diversity is likely to enlarge a lot.
Rather than the magnification parameter, I would look at productivity (numbers of images per hour; or alternatively, numbers of samples per hour) and sample diversity (different kinds of samples imaged). I expect those numbers would indeed allow the FESEM to blow the old W inst out of the water (at least for a typical university research type of facility).
Hope this helps, Tobias
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
My apologies for the repeat post. I need to make a correction to the Box number in the mailing address for Electron Microscopy Specialist Position at Montclair State University.
Stefanie
Electron microscopy specialist position available, Montclair State University
We seek an electron microscopy specialist to support research and teaching efforts at Montclair State University. The specialist will instruct students, faculty, and visitors in the use of our Hitachi S2460N and S3400N Scanning Electron Microscopes, H7500 Transmission Electron Microscope, and supporting sample preparation equipment. The successful applicant will have expertise in the preparation, imaging, and compositional analysis of natural and synthetic materials, experience with maintenance and trouble-shooting of electron microscopes and their related equipment, and excellent communication and interpersonal skills with the ability to work in a multidisciplinary facility with a wide variety of beginning through advanced users.
Responsibilities of the position include: - Training new users on our three electron microscopes and their supporting equipment - Conducting basic preventative maintenance on the electron microscopes, diagnosing instrument malfunctions, and coordinating with repair personnel - Overseeing chemical hygiene and chemical safety in the electron microscopy facility - Working the facility directors on purchasing and billing - Organizing and teaching workshops and short courses for internal and external users - Participating in university outreach efforts - Soliciting external consulting work and/or soliciting external research funding
A masters degree in a STEM discipline and at least 3 years of experience in the operation and maintenance of electron microscopes and their sample preparation equipment is required. Strong communication skills, significant experience in the preparation, imaging, and analysis of a wide array of sample types is required. Strong knowledge of computers, electronics, and troubleshooting is desirable. The successful candidate will be qualified to conduct consulting work and/or pursue external research funding. Experience with other major analytical equipment is a plus. The preferred start data is August 1, 2011.
A CV, statement of technical skills, research, laboratory management experience and relevant accomplishments, any supplementary materials (reprints, URLs, etc.) and the names and contact information for four references should be sent to brachfelds-at-mail.montclair.edu (email applications preferred). Hard copy applications should be sent to Dr. Stefanie Brachfeld, Department of Earth and Environmental Studies, Box V589, Montclair State University, Montclair, NJ 07043. Review of applications will be on March 28, 2011.
Please visit us at www.csam.montclair.edu
==============================Original Headers============================== 10, 35 -- From brachfelds-at-mail.montclair.edu Fri Mar 4 15:26:12 2011 10, 35 -- Received: from smtp.montclair.edu (smtp.montclair.edu [130.68.1.64]) 10, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p24LQC41023446 10, 35 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 15:26:12 -0600 10, 35 -- Received: from smtp.montclair.edu (localhost [127.0.0.1]) 10, 35 -- by localhost (Postfix) with SMTP id 4562080E43 10, 35 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 16:26:12 -0500 (EST) 10, 35 -- Received: from mta3.montclair.edu (mail.montclair.edu [10.1.106.84]) 10, 35 -- by smtp.montclair.edu (Postfix) with ESMTP id F332E80E3F 10, 35 -- for {microscopy-at-microscopy.com} ; Fri, 4 Mar 2011 16:26:11 -0500 (EST) 10, 35 -- MIME-version: 1.0 10, 35 -- Content-disposition: inline 10, 35 -- Content-type: text/plain; charset=iso-8859-1 10, 35 -- Received: from mailstore1.montclair.edu ([130.68.1.20]) 10, 35 -- by mta3.montclair.edu (Sun Java(tm) System Messaging Server 6.3-11.01 (built 10, 35 -- Feb 12 2010; 32bit)) with ESMTP id {0LHJ00M9MY7NTU00-at-mta3.montclair.edu} for 10, 35 -- microscopy-at-microscopy.com; Fri, 04 Mar 2011 16:26:11 -0500 (EST) 10, 35 -- Received: from montclair.edu ([10.1.106.81]) 10, 35 -- by ms0.montclair.edu (Sun Java(tm) System Messaging Server 6.3-11.01 (built 10, 35 -- Feb 12 2010; 32bit)) with ESMTP id {0LHJ00FMRY7N5UA0-at-ms0.montclair.edu} for 10, 35 -- microscopy-at-microscopy.com; Fri, 04 Mar 2011 16:26:11 -0500 (EST) 10, 35 -- Received: from [10.1.106.70 by ms0.montclair.edu (mshttpd); Fri, 10, 35 -- 04 Mar 2011 16:26:11 -0500 10, 35 -- From: "Stefanie A. Brachfeld" {brachfelds-at-mail.montclair.edu} 10, 35 -- To: microscopy-at-microscopy.com 10, 35 -- Message-id: {f817905834a3.4d7112a3-at-montclair.edu} 10, 35 -- Date: Fri, 04 Mar 2011 16:26:11 -0500 10, 35 -- X-Mailer: Sun Java(tm) System Messenger Express 6.3-11.01 (built Feb 12 2010; 10, 35 -- 32bit) 10, 35 -- Content-language: en 10, 35 -- Subject: correction to mailing address: electron microscopy position 10, 35 -- X-Accept-Language: en 10, 35 -- Priority: normal 10, 35 -- Content-Transfer-Encoding: 8bit 10, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p24LQC41023446 ==============================End of - Headers==============================
Hi Debby, I do this sort of thin film work all the time using EPMA here in Oregon by running multiple voltage analyses and correcting for both chemistry and geometry. In this case I would run 3 different electron beam energies probably between 5 and 15 keV and use L lines for the metals. With WDS the Mo-S overlap isn't bad and can be corrected easily anyway. The EPMA is great for quantification, especially for thin films because of its WDS sensitivity.
In my view EPMA is quite underutilized by materials people, but here at UofO it is about 80% of my EPMA workload because of the strong thin film research program.
I'd be happy to run it for you on our Sx50 or Sx100 at our usual academic rates. Contact me off-line if you are interested or just want to chat about the analysis. john
At 10:49 AM 3/4/2011, dsherman-at-purdue.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
John J. Donovan donovan-at-uoregon.edu University of Oregon (541) 346-4632 (office) 1443 E. 13th Ave (541) 346-4655 (lab) Eugene, OR 97403-1241
Does anyone have a manual for a Varian 880RS gauge controller? I've scoured the 'Net, and Google has failed me. Ahh, Google, how could you have let me down so?
Thanks in advance, and may all of you who are "Out of the office" be having a good time. :)
--Justin A. Kraft
==============================Original Headers============================== 3, 37 -- From kraftpiano-at-gmail.com Mon Mar 7 00:36:23 2011 3, 37 -- Received: from mail-gx0-f169.google.com (mail-gx0-f169.google.com [209.85.161.169]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p276aNbB019444 3, 37 -- for {microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 00:36:23 -0600 3, 37 -- Received: by gxk2 with SMTP id 2so1987204gxk.0 3, 37 -- for {microscopy-at-microscopy.com} ; Sun, 06 Mar 2011 22:36:23 -0800 (PST) 3, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 37 -- d=gmail.com; s=gamma; 3, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 3, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 3, 37 -- bh=rVrpmnLv61EsFL0WKkmCx1Meii1e7dLSlipiKmsPxUY=; 3, 37 -- b=H02W44cx7jlc4xzZv+iaSrIuDeGzEzGIFPZwGGA43fg7B/vvpNggl0GrWCZJWwOEV1 3, 37 -- 6/bS7Fu5uZicwvI6nzUBCdU21RbnZAiQqMyEgdwJS8RWMP8GxIMtojSrJswhM2Qzmp3D 3, 37 -- VBFLzGI830vdhMv18Gi0/UqT3xjDXM5/smB+w= 3, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 37 -- d=gmail.com; s=gamma; 3, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 3, 37 -- :to:mime-version:x-mailer; 3, 37 -- b=be3rjZ60Vp6rieFKGAndsiz0HxZQNoiueAyT6fCGZA6VRsDGB7R28yDuPrZcmuDsig 3, 37 -- COQ4JjLkXd1eef16dPNEOt/UzftGL9iqtHxf3nFVoyXu89gxqL+ah8guUurwPKENAMSU 3, 37 -- y/v8gEBHOSuzcMiJARFFkIyJZDuvGxJgODU6A= 3, 37 -- Received: by 10.90.61.39 with SMTP id j39mr4723994aga.17.1299479783386; 3, 37 -- Sun, 06 Mar 2011 22:36:23 -0800 (PST) 3, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 3, 37 -- by mx.google.com with ESMTPS id x37sm3384717ana.28.2011.03.06.22.36.22 3, 37 -- (version=TLSv1/SSLv3 cipher=OTHER); 3, 37 -- Sun, 06 Mar 2011 22:36:22 -0800 (PST) 3, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 37 -- Content-Type: text/plain; charset=us-ascii 3, 37 -- Subject: Manual request 3, 37 -- Date: Mon, 7 Mar 2011 01:36:20 -0500 3, 37 -- Message-Id: {62B7C25B-98DC-4CFD-B220-521650BBBA8E-at-gmail.com} 3, 37 -- To: microscopy-at-microscopy.com 3, 37 -- Mime-Version: 1.0 (Apple Message framework v1082) 3, 37 -- X-Mailer: Apple Mail (2.1082) 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p276aNbB019444 ==============================End of - Headers==============================
Is there anyone who has the english translation of this paper: Z. Naturforschung (1966), 21a, 476-478 by F. Thon? Thanks Patrizia Canton
-- ______________________________________________ Dr. Patrizia Canton Dept. of Molecular Sciences and Nanosystems Via Torino 155/b I-30170 Venezia-Mestre Italy Phone +39-041-2346790 Fax +39-041-2346747 e-mail cantonpa-at-unive.it ______________________________________________
==============================Original Headers============================== 3, 35 -- From cantonpa-at-unive.it Mon Mar 7 06:02:10 2011 3, 35 -- Received: from ariel.unive.it (ariel.unive.it [157.138.1.34]) 3, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p27C2AAj011347 3, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 06:02:10 -0600 3, 35 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 3, 35 -- by ariel.unive.it (8.12.11.20060308/8.12.11) with ESMTP id p27C2741024396 3, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 13:02:07 +0100 3, 35 -- Received: from ariel.unive.it ([127.0.0.1]) 3, 35 -- by localhost (ariel.unive.it [127.0.0.1]) (amavisd-new, port 10024) 3, 35 -- with LMTP id E6bQ8FpGb1f6 for {Microscopy-at-microscopy.com} ; 3, 35 -- Mon, 7 Mar 2011 13:02:06 +0100 (CET) 3, 35 -- Received: from fibre15.unive.it (fibre15.unive.it [157.138.8.15]) 3, 35 -- by ariel.unive.it (8.12.11.20060308/8.12.11) with ESMTP id p27C24ba024389 3, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 13:02:04 +0100 3, 35 -- Received: from fibre15.unive.it (localhost.localdomain [127.0.0.1]) 3, 35 -- by fibre15.unive.it (8.13.1/8.13.1) with ESMTP id p27C255f002676 3, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 13:02:05 +0100 3, 35 -- Received: (from apache-at-localhost) 3, 35 -- by fibre15.unive.it (8.13.1/8.13.1/Submit) id p27C25Qt002674; 3, 35 -- Mon, 7 Mar 2011 13:02:05 +0100 3, 35 -- Received: from 157.138.17.7 3, 35 -- (SquirrelMail authenticated user cantonpa) 3, 35 -- by helios1.unive.it with HTTP; 3, 35 -- Mon, 7 Mar 2011 13:02:05 +0100 (CET) 3, 35 -- Message-ID: {49912.157.138.17.7.1299499325.squirrel-at-helios1.unive.it} 3, 35 -- Date: Mon, 7 Mar 2011 13:02:05 +0100 (CET) 3, 35 -- Subject: TEM english translation of F. Thon paper 3, 35 -- From: "Patrizia Canton" {cantonpa-at-unive.it} 3, 35 -- To: Microscopy-at-microscopy.com 3, 35 -- User-Agent: SquirrelMail/1.4.8-5.el4_8.8 3, 35 -- MIME-Version: 1.0 3, 35 -- Content-Type: text/plain;charset=utf-8 3, 35 -- Content-Transfer-Encoding: 8bit 3, 35 -- X-Priority: 3 (Normal) 3, 35 -- Importance: Normal ==============================End of - Headers==============================
Hi Patrizia, Years ago, I found a translation of a paper that I needed from the "world translation index" (WTI). This is a compendium of journal article translations from all over the world, indexed by journal. I found this in the pre-on-line-everthing days, in the reference section of my university library. I expect it is still there. This is good old fashioned reference hunting -- the WTI was published once a year, indexing the translations appearing over the past year, and so I needed to pull down each vol starting after the year my target came out, flip through the pages to find my source journal, and then scan down through the various listings within that journal to see if my volume and page numbers had a hit. Repeat with the next volume. The hit in question provided information to a translation held in a library (in Birmingham I think it was) the the library was able to request for me. All delightfully old world, I know.
I have no idea how or if this has survived or transformed into a web presence today. I did just now look online briefly, and found that UNESCO maintains something called "Index Translationum". Apparently you can search this for papers after 1979 (not much help for your quest) but they also mention that earlier papers are in the printed version and they have some links about that. I am guessing this is the same thing I looked at. But I just searched quickly.
Sorry if you know all about this -- it was certainly news to me when I found it (and by the way the translation that I tracked down from WTI turned out to be a good one).
As ever Tobias
At 6:02 AM -0600 3/7/11, cantonpa-at-unive.it wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Listers, our Jeol JSM-5600LV has a problem with the vacuum system. In particular, it doesn't vent the chamber. Maybe it's a problem of some valves, but before dismantle and check them I'd prefer to vent the system in a way safe for the EDS window. Does anyone know a safe way to vent manually? Thanks
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 7, 18 -- From dcristofori-at-unive.it Mon Mar 7 10:23:47 2011 7, 18 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p27GNlNc018343 7, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 10:23:47 -0600 7, 18 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 7, 18 -- (authenticated bits=0) 7, 18 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p27GNjIY026022 7, 18 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 7, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 17:23:46 +0100 7, 18 -- Message-ID: {4D7506CA.3040506-at-unive.it} 7, 18 -- Date: Mon, 07 Mar 2011 17:24:42 +0100 7, 18 -- From: Davide Cristofori {dcristofori-at-unive.it} 7, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; it; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 7, 18 -- MIME-Version: 1.0 7, 18 -- To: Microscopy-at-microscopy.com 7, 18 -- Subject: SEM: venting procedure failed 7, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 7, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Is there any error message ? Have you check securities of the microscope ? Some of them inhibit vacuum system like water cooling. Primary vacuum may be bad also (check oil level and quality of the rotary pump and the Vbelt too). There is check points on vacuum card to measure vacuum levels from pirani gauges it could be interesting to test that. Sometime just a small adjustment of potentiometer is enough. Under column, there is a big valve with a switch on it axis . This switch is also cause of security alert in vacuum sequence. Axis may be slowed down by old grease. In my opinion, venting the chamber is not urgent to check vacuum and not safe for EDS window. I recommend to diagnose the problem before open...otherwise your SEM may want to pump the chamber anyway. If it's necessary, you can try to break vacuum from the top of the column (gun side) to avoid a big "wind" in the chamber, but this must be done very carefully and after swich off the SEM. I hope this could be help for you.
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
On my old JEOL 840, This happens when the compressed nitrogen tank that provides the gas to vent the system runs out. Since a cylinder lasts for years, it is easy to forget about.
Dave
On 3/7/2011 11:26 AM, dcristofori-at-unive.it wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } our Jeol JSM-5600LV has a problem with the vacuum system. } In particular, it doesn't vent the chamber. Maybe it's a problem of some } valves, but before dismantle and check them I'd prefer to vent the } system in a way safe for the EDS window. } Does anyone know a safe way to vent manually? } Thanks } } Davide } } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Universita' Ca' Foscari Venezia } Dipartimento di Sc. Molecolari e Nanosistemi } Lab. di Scienza e Tecnologia dei Materiali } Via Torino, 155b } I-30172 Mestre (VE) } Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } ==============================Original Headers============================== } 7, 18 -- From dcristofori-at-unive.it Mon Mar 7 10:23:47 2011 } 7, 18 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) } 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p27GNlNc018343 } 7, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 10:23:47 -0600 } 7, 18 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) } 7, 18 -- (authenticated bits=0) } 7, 18 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p27GNjIY026022 } 7, 18 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) } 7, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Mar 2011 17:23:46 +0100 } 7, 18 -- Message-ID: {4D7506CA.3040506-at-unive.it} } 7, 18 -- Date: Mon, 07 Mar 2011 17:24:42 +0100 } 7, 18 -- From: Davide Cristofori {dcristofori-at-unive.it} } 7, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; it; rv:1.9.2.13) Gecko/20101207 Thunderbird/3.1.7 } 7, 18 -- MIME-Version: 1.0 } 7, 18 -- To: Microscopy-at-microscopy.com } 7, 18 -- Subject: SEM: venting procedure failed } 7, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 7, 18 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- __________________________________ David J. Wilbur, Ph.D. Instrumentation Specialist Department of Chemistry Tufts University 62 Talbot Ave. Medford, MA 02155 voice: 617-627-2163 Fax: 617-627-3443 email: david.wilbur-at-tufts.edu __________________________________
==============================Original Headers============================== 5, 24 -- From David.Wilbur-at-tufts.edu Tue Mar 8 07:19:54 2011 5, 24 -- Received: from poplar.usg.tufts.edu (poplar.usg.tufts.edu [130.64.1.56]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p28DJspf019106 5, 24 -- for {Microscopy-at-Microscopy.Com} ; Tue, 8 Mar 2011 07:19:54 -0600 5, 24 -- Received: from tftmexcashub2.tufts.ad.tufts.edu ([130.64.10.182]:15281) 5, 24 -- by poplar.usg.tufts.edu with esmtps (TLSv1:AES128-SHA:128) 5, 24 -- (Exim 4.60) 5, 24 -- (envelope-from {David.Wilbur-at-tufts.edu} ) 5, 24 -- id 1PwwpN-0005WV-ny; Tue, 08 Mar 2011 08:19:53 -0500 5, 24 -- Received: from [130.64.102.176] (130.64.102.176) by 5, 24 -- TFTMEXCASHUB2.tufts.ad.tufts.edu (130.64.10.179) with Microsoft SMTP Server 5, 24 -- (TLS) id 14.1.218.12; Tue, 8 Mar 2011 08:19:53 -0500 5, 24 -- Message-ID: {4D762CF9.5060404-at-tufts.edu} 5, 24 -- Date: Tue, 8 Mar 2011 08:19:53 -0500 5, 24 -- From: David Wilbur {david.wilbur-at-tufts.edu} 5, 24 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.15) Gecko/20110303 Lightning/1.0b2 Thunderbird/3.1.9 5, 24 -- MIME-Version: 1.0 5, 24 -- To: {dcristofori-at-unive.it} , Microscopy Listserver {Microscopy-at-Microscopy.Com} 5, 24 -- Subject: Re: [Microscopy] SEM: venting procedure failed 5, 24 -- References: {201103071626.p27GQfXL022296-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {201103071626.p27GQfXL022296-at-ns.microscopy.com} 5, 24 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Originating-IP: [130.64.102.176] ==============================End of - Headers==============================
I am looking for a working protocol preparing cells for standard morphological TEM. So far I have read many different ways of doing this but have never actually prepared cells for TEM. I was hoping to find out peoples preferences, whether they fix cells in a monolayer, or a suspension, or just fix the pellet. I was also wondering if people like to pre-embed in agar, and what cell concentrations are preferred to start with. Any help would be great.
Thanks,
Michael
==============================Original Headers============================== 9, 37 -- From prvs=1048fe0e50=mmashore-at-vapop.ucsd.edu Tue Mar 8 15:46:11 2011 9, 37 -- Received: from iport-c2-out.ucsd.edu (outbound.ucsd.edu [132.239.0.119]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p28LkB3k016199 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Mar 2011 15:46:11 -0600 9, 37 -- X-IronPort-Anti-Spam-Filtered: true 9, 37 -- X-IronPort-Anti-Spam-Result: AoMFAN4ydk2E71Ri/2dsb2JhbACJfI5+jW10t2KIa4VjBIUd 9, 37 -- X-IronPort-AV: E=Sophos;i="4.62,286,1297065600"; 9, 37 -- d="scan'208";a="453274563" 9, 37 -- X-Spam-Status: No 9, 37 -- X-Spam-Level: 9, 37 -- Received: from vapop.ucsd.edu ([132.239.84.98]) 9, 37 -- by iport-c2-out.ucsd.edu with ESMTP; 08 Mar 2011 13:45:56 -0800 9, 37 -- Received: from vandeberg by vapop.ucsd.edu (MDaemon PRO v9.6.6) 9, 37 -- with ESMTP id md50002066177.msg 9, 37 -- for {Microscopy-at-Microscopy.Com} ; Tue, 08 Mar 2011 13:44:33 -0800 9, 37 -- X-Spam-Processed: vapop.ucsd.edu, Tue, 08 Mar 2011 13:44:33 -0800 9, 37 -- (not processed: message from trusted or authenticated source) 9, 37 -- X-Return-Path: prvs=1048fe0e50=mmashore-at-vapop.ucsd.edu 9, 37 -- X-Envelope-From: mmashore-at-vapop.ucsd.edu 9, 37 -- X-MDaemon-Deliver-To: Microscopy-at-Microscopy.Com 9, 37 -- From: "Michael Mashore" {mmashore-at-vapop.ucsd.edu} 9, 37 -- To: {Microscopy-at-microscopy.com} 9, 37 -- Subject: Protocol for TEM of Cultured Cells 9, 37 -- Date: Tue, 8 Mar 2011 13:45:18 -0800 9, 37 -- Message-ID: {0794340FDC484A808EA515C6D79EA9C9-at-vandeberg} 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; 9, 37 -- charset="us-ascii" 9, 37 -- X-Priority: 3 (Normal) 9, 37 -- X-MSMail-Priority: Normal 9, 37 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 9, 37 -- Importance: Normal 9, 37 -- Thread-Index: Acvd2h2wgGkHrOWeSYq7PIowIEA3tw== 9, 37 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 9, 37 -- X-MDAV-Processed: vapop.ucsd.edu, Tue, 08 Mar 2011 13:44:34 -0800 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p28LkB3k016199 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both csuarez000-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: csuarez000-at-gmail.com Name: Cristina
Title-Subject: [Filtered] yeast like cryoprotectant in HPF
Message: Dear colleagues,
I am working with virus infected mamamaliam cells using HPF and cryosubstitution. I have same problems with the preservation and cryoprotectants. Some people told me that I can use yeast like cryoprotectant. I have no experience in this feld. Have some of you worked with this method, and can help me? Thanks so much
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea.greene-at-wdc.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: andrea.greene-at-wdc.com Name: Andrea Greene
Organization: Western Digital
Title-Subject: [Filtered] TEM Specialist Needed
Message: Successful candidate will utilize their TEM expertise to perform advanced and routine evaluation of disk drive media products for R &D, and defect failure analysis evaluation, reporting the findings in a clearly documented form. They will interact with magnetic research engineers to understand and supply necessary structural information. They will be responsible for equipment uptime, including interfacing with equipment vendor, routine calibration and maintenance of the TEM lab and supplies. As a Sr Engineer, they will be the lead in developing/refining advanced TEM/STEM/EDX/EELS techniques and training Jr members of the Materials Analysis group.
Please apply through http://www.westerndigital.apply2jobs.com job MMO4428
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bob.price-at-uscmed.sc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: The University of South Carolina School of Medicine Instrumentation Resource Facility will be hosting the 7th Annual Workshop on Basic Confocal Microscopy from June 13th through the 17th. The workshop is directed towards beginning and intermediate users of confocal microscopes and involves a series of lectures (specimen preparation, labeling strategies, proper set-up of instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point scanning and spinning disk confocal microscopes. Companies scheduled to have instruments and applications experts on-site include Leica, Nikon, Olympus, Perkin Elmer, Zeiss and Intelligent Imaging Innovations (3i). Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the Medical University of Wisconsin, and Dr. Bob Price of the University of South Carolina. For further information or to register please see: http://dba.med.sc.edu/price/irf/irf.htm or contact Anna McNeal (anna.mcneal-at-uscmed.sc.edu) or Bob Price (bob.price-at-uscmed.sc.edu).
Bob Price Research Professor USC School of Medicine 6439 Garner's Ferry Road Columbia, SC 29209 803-216-3824 (T) 803-216-3825 (Admin Asst) Bob.Price-at-uscmed.sc.edu
We had almost stopped celebrating the Superbowl success of the mighty Packers from tiny Green Bay, Wisconsin, when (what we hope is) the first stroke against the final stage of the rightwing takeover of the US began right here in Madison. (Put "Madison" into the search bar at www.guardian.co.uk or try http://www.defendwisconsin.org/ Please excuse off topic comment.)
In all the excitement (and shovelling the snow!), I almost forgot to get out the second announcement about the 16th UBC Live-Cell Course.
There is usually a bit of a rush as the March 15 deadline approaches, so if you are thinking about applying, now would be a good time (and if you have already taken the Course, please tell your friends!). Go to:
http://3dcourse.ubc.ca/2011/public.php?page=apply
Even if you are still working out the finances, it might still be good to "get in line" by filling out an application.
Next June, in addition to the faculty listed on the brochure, we finally arranged for Kevin Eliceri (of LOCI fame: http://www.loci.wisc.edu/people/kevin-eliceiri) to come and talk about image database management and other matters. With Rachel Wong (http://wonglab.biostr.washington.edu/), this makes at least 2 new faces this year, and there may still be more coming.
We don't yet know all the equipment that will be there but I have just heard that Leica expects to bring a TIRF and an SP5x confocal system this year, Zeiss will probably send an LSM-5-Live, and we expect a Nikon TIRF system and an AI confocal. Yokogawa/Quorum itself will bring their new CV1000, an all-in-one, bench-top confocal imaging system for long-term, multi-point, 3D, time-lapse imaging. We also expect Olympus to send a TIRF and an FV10 Integrated Confocal System ("confocal in a box") and their new spectral scanner with FRAP-uncaging kit. Quorum will bring the Yokogawa CV1000.
This is not close to a complete list, just those we have heard details about. We also expect systems from from Applied Precision, McBain Instruments/Visitech, Bit-Plane, PerkinElmer, and perhaps TILL. As they come in, they will be described at
Overall, we expect to have access to at least thirteen 3D systems.
Remember, these systems are not just there for demos. Students will use then for 14, 2-hour lab sessions over 5 days working with an advisor in one of 8 small groups.
Many folks have found it a great way to really try out all the new equipment in one convenient location before committing to a purchase.
Hope to see you there!
Jim P. -- *************************************************************************** Prof. James B. Pawley, Ph. 608-238-3953 21. N. Prospect Ave. Madison, WI 53726 USA JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 11-23, 2011, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2011 "If it ain't diffraction, it must be statistics." Anon.
To sign up, click on: http://www.3dcourse.ubc.ca/2011/public.php?page=apply
==============================Original Headers============================== 17, 28 -- From jbpawley-at-wisc.edu Tue Mar 8 20:51:55 2011 17, 28 -- Received: from agogare.doit.wisc.edu (agogare.doit.wisc.edu [144.92.197.211]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p292ptuD022540 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 8 Mar 2011 20:51:55 -0600 17, 28 -- MIME-version: 1.0 17, 28 -- Content-transfer-encoding: 7BIT 17, 28 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 17, 28 -- Received: from avs-daemon.smtpauth2.wiscmail.wisc.edu by 17, 28 -- smtpauth2.wiscmail.wisc.edu 17, 28 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 17, 28 -- id {0LHR00I00RYJ7D00-at-smtpauth2.wiscmail.wisc.edu} for 17, 28 -- microscopy-at-microscopy.com; Tue, 08 Mar 2011 20:51:55 -0600 (CST) 17, 28 -- Received: from [12.105.165.116] 17, 28 -- (99-126-179-197.lightspeed.mdsnwi.sbcglobal.net [99.126.179.197]) 17, 28 -- by smtpauth2.wiscmail.wisc.edu 17, 28 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 17, 28 -- with ESMTPSA id {0LHR00GAORYHKR00-at-smtpauth2.wiscmail.wisc.edu} ; Tue, 17, 28 -- 08 Mar 2011 20:51:54 -0600 (CST) 17, 28 -- Date: Tue, 08 Mar 2011 20:51:51 -0600 17, 28 -- From: James Pawley {jbpawley-at-wisc.edu} 17, 28 -- Subject: Second Notice: Sixteenth International UBC 3D Live-Cell Course next 17, 28 -- June 17, 28 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 17, 28 -- Message-id: {p06240879c99bda376a0b-at-[12.105.165.116]} 17, 28 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=12.105.165.116 17, 28 -- X-Spam-PmxInfo: Server=avs-9, Version=5.6.0.2009776, 17, 28 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.3.9.22719, 17, 28 -- SenderIP=12.105.165.116 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sc-at-nanoct.com.ar as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sc-at-nanoct.com.ar Name: Silvio Cechet
Organization: NanoCT
Title-Subject: [Filtered] Electroscan 2020
Message: Hi We found an old Electroscan 2020 but it doesn't have the computer and the program. Can anyone tell me where can I found it?
I really hate to see shameless political propaganda on the list. Disgusting.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: jbpawley-at-wisc.edu [mailto:jbpawley-at-wisc.edu] } Sent: Tuesday, March 08, 2011 8:52 PM } To: Dusevich, Vladimir } Subject: [Microscopy] Second Notice: Sixteenth International UBC 3D } Live-Cell Course next } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Hello all, } } We had almost stopped celebrating the Superbowl success of the mighty } Packers from tiny Green Bay, Wisconsin, when (what we hope is) the } first stroke against the final stage of the rightwing takeover of the } US began right here in Madison. (Put "Madison" into the search bar at } www.guardian.co.uk or try http://www.defendwisconsin.org/ Please } excuse off topic comment.) } } In all the excitement (and shovelling the snow!), I almost forgot to } get out the second announcement about the 16th UBC Live-Cell Course. } } http://3dcourse.ubc.ca/2011/students.php?page=overview } } There is usually a bit of a rush as the March 15 deadline approaches, } so if you are thinking about applying, now would be a good time (and } if you have already taken the Course, please tell your friends!). Go } to: } } http://3dcourse.ubc.ca/2011/public.php?page=apply } } Even if you are still working out the finances, it might still be } good to "get in line" by filling out an application. } } Next June, in addition to the faculty listed on the brochure, we } finally arranged for Kevin Eliceri (of LOCI fame: } http://www.loci.wisc.edu/people/kevin-eliceiri) to come and talk } about image database management and other matters. With Rachel Wong } (http://wonglab.biostr.washington.edu/), this makes at least 2 new } faces this year, and there may still be more coming. } } We don't yet know all the equipment that will be there but I have } just heard that Leica expects to bring a TIRF and an SP5x confocal } system this year, Zeiss will probably send an LSM-5-Live, and we } expect a Nikon TIRF system and an AI confocal. Yokogawa/Quorum } itself will bring their new CV1000, an all-in-one, bench-top confocal } imaging system for long-term, multi-point, 3D, time-lapse imaging. } We also expect Olympus to send a TIRF and an FV10 Integrated Confocal } System ("confocal in a box") and their new spectral scanner with } FRAP-uncaging kit. Quorum will bring the Yokogawa CV1000. } } This is not close to a complete list, just those we have heard } details about. We also expect systems from from Applied Precision, } McBain Instruments/Visitech, Bit-Plane, PerkinElmer, and perhaps } TILL. As they come in, they will be described at } } http://www.3dcourse.ubc.ca/2011/public.php?page=companies, } } Overall, we expect to have access to at least thirteen 3D systems. } } Remember, these systems are not just there for demos. Students will } use then for 14, 2-hour lab sessions over 5 days working with an } advisor in one of 8 small groups. } } Many folks have found it a great way to really try out all the new } equipment in one convenient location before committing to a purchase. } } Hope to see you there! } } Jim P. } -- } *********************************************************************** } **** } Prof. James B. Pawley, Ph. } 608-238-3953 } 21. N. Prospect Ave. Madison, WI 53726 USA } JBPAWLEY-at-WISC.EDU } 3D Microscopy of Living Cells Course, June 11-23, 2011, UBC, Vancouver } Canada } Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, } 2011 } "If it ain't diffraction, it must be statistics." Anon. } } To sign up, click on: } http://www.3dcourse.ubc.ca/2011/public.php?page=apply } } ==============================Original } Headers============================== } 17, 28 -- From jbpawley-at-wisc.edu Tue Mar 8 20:51:55 2011 } 17, 28 -- Received: from agogare.doit.wisc.edu (agogare.doit.wisc.edu } [144.92.197.211]) } 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p292ptuD022540 } 17, 28 -- for {microscopy-at-microscopy.com} ; Tue, 8 Mar 2011 20:51:55 - } 0600 } 17, 28 -- MIME-version: 1.0 } 17, 28 -- Content-transfer-encoding: 7BIT } 17, 28 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed } 17, 28 -- Received: from avs-daemon.smtpauth2.wiscmail.wisc.edu by } 17, 28 -- smtpauth2.wiscmail.wisc.edu } 17, 28 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built } Jul 30 2009)) } 17, 28 -- id {0LHR00I00RYJ7D00-at-smtpauth2.wiscmail.wisc.edu} for } 17, 28 -- microscopy-at-microscopy.com; Tue, 08 Mar 2011 20:51:55 -0600 } (CST) } 17, 28 -- Received: from [12.105.165.116] } 17, 28 -- (99-126-179-197.lightspeed.mdsnwi.sbcglobal.net } [99.126.179.197]) } 17, 28 -- by smtpauth2.wiscmail.wisc.edu } 17, 28 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built } Jul 30 2009)) } 17, 28 -- with ESMTPSA id } {0LHR00GAORYHKR00-at-smtpauth2.wiscmail.wisc.edu} ; Tue, } 17, 28 -- 08 Mar 2011 20:51:54 -0600 (CST) } 17, 28 -- Date: Tue, 08 Mar 2011 20:51:51 -0600 } 17, 28 -- From: James Pawley {jbpawley-at-wisc.edu} } 17, 28 -- Subject: Second Notice: Sixteenth International UBC 3D Live- } Cell Course next } 17, 28 -- June } 17, 28 -- To: Microscopy Listserver {microscopy-at-microscopy.com} } 17, 28 -- Message-id: {p06240879c99bda376a0b-at-[12.105.165.116]} } 17, 28 -- X-Spam-Report: AuthenticatedSender=yes, } SenderIP=12.105.165.116 } 17, 28 -- X-Spam-PmxInfo: Server=avs-9, Version=5.6.0.2009776, } 17, 28 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: } 2011.3.9.22719, } 17, 28 -- SenderIP=12.105.165.116 } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 33 -- From prvs=0424dbd60=DusevichV-at-umkc.edu Wed Mar 9 09:33:50 2011 4, 33 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p29FXocF010641 4, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 09:33:50 -0600 4, 33 -- X-IronPort-Anti-Spam-Filtered: true 4, 33 -- X-IronPort-Anti-Spam-Result: AvQAAPMsd03Raua1/2dsb2JhbACYQY4sdIhirHQBhQyIa4MYgk0EhSKTQQ 4, 33 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) 4, 33 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Mar 2011 09:33:49 -0600 4, 33 -- Received: from um-email06.um.umsystem.edu ([169.254.1.153]) by 4, 33 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Wed, 9 Mar 2011 4, 33 -- 09:33:49 -0600 4, 33 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 4, 33 -- To: "jbpawley-at-wisc.edu" {jbpawley-at-wisc.edu} , 4, 33 -- "Microscopy-at-microscopy.com" 4, 33 -- {Microscopy-at-microscopy.com} 4, 33 -- Date: Wed, 9 Mar 2011 09:33:48 -0600 4, 33 -- Subject: RE: [Microscopy] Second Notice: Sixteenth International UBC 3D 4, 33 -- Live-Cell Course next 4, 33 -- Thread-Topic: [Microscopy] Second Notice: Sixteenth International UBC 3D 4, 33 -- Live-Cell Course next 4, 33 -- Thread-Index: AcveBQAJExhnEWggQPaUP11OSYeC+gAaiOtw 4, 33 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3AE14FB27CF-at-UM-EMAIL06.um.umsystem.edu} 4, 33 -- References: {201103090252.p292qFEe023305-at-ns.microscopy.com} 4, 33 -- In-Reply-To: {201103090252.p292qFEe023305-at-ns.microscopy.com} 4, 33 -- Accept-Language: en-US 4, 33 -- Content-Language: en-US 4, 33 -- X-MS-Has-Attach: 4, 33 -- X-MS-TNEF-Correlator: 4, 33 -- acceptlanguage: en-US 4, 33 -- Content-Type: text/plain; charset="us-ascii" 4, 33 -- MIME-Version: 1.0 4, 33 -- Content-Transfer-Encoding: 8bit 4, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p29FXocF010641 ==============================End of - Headers==============================
Dear Listers, I found the problem was that the piston of a mechanical valve is slowed down, which prevented to complete the venting sequence. I helped the piston movement by pushing it gently, and the sequence could be completed. Now I hope it's enough to clean the valve, though the dismantling won't be that easy. I'd like to thank everybody gave suggestions. I greatly appreciate your help.
Davide
==============================Original Headers============================== 2, 18 -- From dcristofori-at-unive.it Wed Mar 9 11:33:20 2011 2, 18 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p29HXJFV006419 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 11:33:20 -0600 2, 18 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 2, 18 -- (authenticated bits=0) 2, 18 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p29HXIEP002264 2, 18 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 18:33:19 +0100 2, 18 -- Message-ID: {4D77BA1B.6070205-at-unive.it} 2, 18 -- Date: Wed, 09 Mar 2011 18:34:19 +0100 2, 18 -- From: Davide Cristofori {dcristofori-at-unive.it} 2, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; it; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 2, 18 -- MIME-Version: 1.0 2, 18 -- To: Microscopy-at-microscopy.com 2, 18 -- Subject: SEM: venting procedure failed - problem identified 2, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 2, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Title-Subject: [Filtered] yeast like cryoprotectant in HPF
Message: Dear colleagues,
I am working with virus infected mamamaliam cells using HPF and cryosubstitution. I have same problems with the preservation and cryoprotectants. Some people told me that I can use yeast like cryoprotectant. I have no experience in this feld. Have some of you worked with this method, and can help me? Thanks so much
C.
C, I believe thats yeast paste or its supernatant as a cryoprotectant. You can also use hexadecene,0.2M sucrose, 0.3 M Mannitol, 20-25% dextran or 20% BSA, endless choices. I do believe a 2-3% amount of water in your FS cocktail (1% osmium 0.2% UA in 95% acetone 5% methanol) is better. Have you checked out Mary Morphew's manual from Boulder Co.?
http://bio3d.colorado.edu/docs/mmanual.pdf
very useful, good luck.
Mike Delannoy JHMI Microscope Facility Baltimore Md
==============================Original Headers============================== 10, 29 -- From prvs=042b6838a=delannoy-at-jhmi.edu Wed Mar 9 12:54:28 2011 10, 29 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p29IsScI008544 10, 29 -- for {microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 12:54:28 -0600 10, 29 -- X-IronPort-AV: E=Sophos;i="4.62,291,1297054800"; 10, 29 -- d="scan'208";a="85562503" 10, 29 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 10, 29 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 09 Mar 2011 13:54:28 -0500 10, 29 -- MIME-version: 1.0 10, 29 -- Content-transfer-encoding: 7BIT 10, 29 -- Content-disposition: inline 10, 29 -- Content-type: text/plain; CHARSET=US-ASCII 10, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 10, 29 -- by commsmail.johnshopkins.edu 10, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 10, 29 -- with ESMTP id {0LHT00N8B0IR3Y60-at-commsmail.johnshopkins.edu} for 10, 29 -- microscopy-at-microscopy.com; Wed, 09 Mar 2011 13:54:27 -0500 (EST) 10, 29 -- Received: from [10.173.109.14] (Forwarded-For: [10.16.66.49]) 10, 29 -- by commsmail.johnshopkins.edu (mshttpd); Wed, 09 Mar 2011 13:54:27 -0500 10, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 10, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 29 -- Message-id: {7110b6761d17e.4d778693-at-johnshopkins.edu} 10, 29 -- Date: Wed, 09 Mar 2011 13:54:27 -0500 10, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 10, 29 -- 2009) 10, 29 -- Content-language: en 10, 29 -- Subject: HPF 10, 29 -- X-Accept-Language: en 10, 29 -- Priority: normal ==============================End of - Headers==============================
I do not know the type of valve that you are discussing but may I offer some general advice on trying to fix it?
1. Switch off the microscope at the wall. 2. Work with the idea that someone had to build it therefore there must be a way of taking it apart. 3. Have someone with you so that you can dictate what you did to take the unit apart, it will help when reversing the process. 4. Expect a spring under tension to be inside the unit so take great care when finally opening it. 5. A standard reason for such an item to jam is the "O" ring has dried out and it is sticking to the degraded grease within the chamber. 6. Give the chamber and the plunger a good clean and use a good quality vacuum grease on the "0" ring.
Good luck
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 09 March 2011 17:34 To: protrain-at-emcourses.com
Dear Listers, I found the problem was that the piston of a mechanical valve is slowed down, which prevented to complete the venting sequence. I helped the piston movement by pushing it gently, and the sequence could be completed. Now I hope it's enough to clean the valve, though the dismantling won't be that easy. I'd like to thank everybody gave suggestions. I greatly appreciate your help.
Davide
==============================Original Headers============================== 2, 18 -- From dcristofori-at-unive.it Wed Mar 9 11:33:20 2011 2, 18 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 2, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p29HXJFV006419 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 11:33:20 -0600 2, 18 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 2, 18 -- (authenticated bits=0) 2, 18 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p29HXIEP002264 2, 18 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 2, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 18:33:19 +0100 2, 18 -- Message-ID: {4D77BA1B.6070205-at-unive.it} 2, 18 -- Date: Wed, 09 Mar 2011 18:34:19 +0100 2, 18 -- From: Davide Cristofori {dcristofori-at-unive.it} 2, 18 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; it; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 2, 18 -- MIME-Version: 1.0 2, 18 -- To: Microscopy-at-microscopy.com 2, 18 -- Subject: SEM: venting procedure failed - problem identified 2, 18 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 2, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 14, 27 -- From protrain-at-emcourses.com Wed Mar 9 13:15:32 2011 14, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p29JFVaa029218 14, 27 -- for {microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 13:15:32 -0600 14, 27 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:55648 helo=ProtrainPC) 14, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 14, 27 -- (envelope-from {protrain-at-emcourses.com} ) 14, 27 -- id 1PxOr2-00033R-Mu by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 09 Mar 2011 21:15:29 +0200 14, 27 -- Reply-To: {protrain-at-emcourses.com} 14, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 14, 27 -- To: {dcristofori-at-unive.it} 14, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 14, 27 -- References: {201103091733.p29HXxJa007799-at-ns.microscopy.com} 14, 27 -- In-Reply-To: {201103091733.p29HXxJa007799-at-ns.microscopy.com} 14, 27 -- Subject: RE: [Microscopy] SEM: venting procedure failed - problem identified 14, 27 -- Date: Wed, 9 Mar 2011 19:15:20 -0000 14, 27 -- Organization: Protrain 14, 27 -- Message-ID: {002e01cbde8e$57aeb1e0$070c15a0$-at-com} 14, 27 -- MIME-Version: 1.0 14, 27 -- Content-Type: text/plain; 14, 27 -- charset="us-ascii" 14, 27 -- Content-Transfer-Encoding: 7bit 14, 27 -- X-Mailer: Microsoft Office Outlook 12.0 14, 27 -- Thread-Index: AcvegDqRKEA0/oJ6SxeljDg8bUlr4AADGQ3Q 14, 27 -- Content-Language: en-gb 14, 27 -- X-Scan-Signature: 0c67312a1104bb911f29df56131c35d9{80}} 14, 27 -- X-Trace: smtp01.dial-up.net 1PxOr2-00033R-Mu eb5d03f96d94b9ddfe84f831690ce809 ==============================End of - Headers==============================
Maybe I'm just slowing down in my old age, but I thought what Jim Pawley said was a pretty blatant spoof, or shot at the political system as designed by columnists, not a statement of politics. Rather than get bent out of shape, we should see it as it is and get a bit of a chuckle. Unless, of course, you were cheering for the Steelers. But since neither the Vikes nor Steelers play real (read Canadian) football - who really cares?
Paul
-- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 Bannatyne Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 20 -- From paul_hazelton-at-umanitoba.ca Wed Mar 9 17:45:11 2011 5, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p29NjBbl029352 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 17:45:11 -0600 5, 20 -- Received: from [192.168.100.100] (wnpgmb014rw-ad01-103-69.dynamic.mtsallstream.net [207.161.103.69]) 5, 20 -- (user=hazeltn mech=LOGIN bits=0) 5, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p29NjAbr027726; 5, 20 -- Wed, 9 Mar 2011 17:45:11 -0600 (CST) 5, 20 -- Message-ID: {4D781106.9000708-at-umanitoba.ca} 5, 20 -- Date: Wed, 09 Mar 2011 17:45:10 -0600 5, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 20 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.0; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 5, 20 -- MIME-Version: 1.0 5, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 5, 20 -- Subject: Re: [Microscopy] Second Notice: Sixteenth International UBC 3D 5, 20 -- References: {201103091702.p29H2PR4018285-at-ns.microscopy.com} 5, 20 -- In-Reply-To: {201103091702.p29H2PR4018285-at-ns.microscopy.com} 5, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 20 -- Content-Transfer-Encoding: 7bit 5, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
I seem to remember this topic being discussed before. I hope you all not to mind that I am bringing it up again.
Can I get opinions from experts out there on the comparison between CPD and HMDS drying for various types of SEM samples? If there are artifact associated with each of this drying method, what do they look like? Since attachments are not allowed on this site, please feel free to email me if you have any images to share. Thank you very much in advance for your help.
Hong
This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.
If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
==============================Original Headers============================== 7, 36 -- From hyi-at-emory.edu Wed Mar 9 18:17:11 2011 7, 36 -- Received: from mr2.cc.emory.edu (mr2.cc.emory.edu [170.140.52.91]) 7, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2A0HAjG013383 7, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 18:17:10 -0600 7, 36 -- Received: from EXCHEDGE1.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 7, 36 -- by mr2.cc.emory.edu (8.13.1/8.13.1) with ESMTP id p2A0H86R000946 7, 36 -- for {Microscopy-at-microscopy.com} ; Wed, 9 Mar 2011 19:17:08 -0500 7, 36 -- Received: from EXCHHUB1.Enterprise.emory.net (170.140.30.53) by 7, 36 -- EXCHEDGE1.enterprise.emory.net (170.140.52.33) with Microsoft SMTP Server 7, 36 -- (TLS) id 8.3.137.0; Wed, 9 Mar 2011 19:17:13 -0500 7, 36 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 7, 36 -- EXCHHUB1.Enterprise.emory.net ([170.140.30.53]) with mapi; Wed, 9 Mar 2011 7, 36 -- 19:17:08 -0500 7, 36 -- From: "Yi, Hong" {hyi-at-emory.edu} 7, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 36 -- Date: Wed, 9 Mar 2011 19:17:07 -0500 7, 36 -- Subject: CPD vs HMDS 7, 36 -- Thread-Topic: CPD vs HMDS 7, 36 -- Thread-Index: AQHL3rh+hUVfxzsR1UaguRI4xN/mkw== 7, 36 -- Message-ID: {3940C1839888A54DAE85AE20F294FB690130F2AB275C-at-EXCHANGE12.Enterprise.emory.net} 7, 36 -- Accept-Language: en-US 7, 36 -- Content-Language: en-US 7, 36 -- X-MS-Has-Attach: 7, 36 -- X-MS-TNEF-Correlator: 7, 36 -- acceptlanguage: en-US 7, 36 -- Content-Type: text/plain; charset="us-ascii" 7, 36 -- MIME-Version: 1.0 7, 36 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 7, 36 -- X-Emory-MailScanner-ID: p2A0H86R000946 7, 36 -- X-Emory-MailScanner: Found to be clean 7, 36 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, score=0, 7, 36 -- required 8, autolearn=disabled) 7, 36 -- X-Emory-MailScanner-From: hyi-at-emory.edu 7, 36 -- X-Spam-Status: No 7, 36 -- Content-Transfer-Encoding: 8bit 7, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2A0HAjG013383 ==============================End of - Headers==============================
I am working at the moment with cells for standard EM. I have monolayer of cells (these attach to glass goverslip) and a suspension of cells (which do not attach to glass coverslip). I prepare them through a standard protocol (glut/PFA, OsO4, uranyl acetate, dehydration, resin infiltration, resin polymerisation). If I work with the monolayers, I do everything (up to the resin) in a culture dish or a petri. For the suspension, I first pellet them and then process in the same way as the others. Just trying to be extremely careful not to disturb the pellet. In both cases, I try to exchange the solutions very carefully and slowly. Both cell types are from mouse. I too would like to hear/read what other people have to say on this. It would be nice to hear other versions of the protocol and try to compare the results. So, if there is anyone that has successfully implemented some modifications in the protocol, please do share your experience. Michael, how is the pre-embedding in Agar done and for type of cells?
Regards,
Josif
(It's so nice to see that so many people on vacation at the moment! I guess that my mailbox will be pleased to receive them all.)
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS) Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y Dpto. Fisiologia Médica y Biofísica Universidad de Sevilla Facultad de Medicina Avda. Sánchez-Pizjuán 4 41009-Sevilla
-----Original Message----- X-from: mmashore-at-vapop.ucsd.edu [mailto:mmashore-at-vapop.ucsd.edu] Sent: Tuesday, March 08, 2011 10:56 PM To: jmircheski-at-us.es
Hello all,
I am looking for a working protocol preparing cells for standard morphological TEM. So far I have read many different ways of doing this but have never actually prepared cells for TEM. I was hoping to find out peoples preferences, whether they fix cells in a monolayer, or a suspension, or just fix the pellet. I was also wondering if people like to pre-embed in agar, and what cell concentrations are preferred to start with. Any help would be great.
Thanks,
Michael
==============================Original Headers============================== 9, 37 -- From prvs=1048fe0e50=mmashore-at-vapop.ucsd.edu Tue Mar 8 15:46:11 2011 9, 37 -- Received: from iport-c2-out.ucsd.edu (outbound.ucsd.edu [132.239.0.119]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p28LkB3k016199 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Mar 2011 15:46:11 -0600 9, 37 -- X-IronPort-Anti-Spam-Filtered: true 9, 37 -- X-IronPort-Anti-Spam-Result: AoMFAN4ydk2E71Ri/2dsb2JhbACJfI5+jW10t2KIa4VjBIUd 9, 37 -- X-IronPort-AV: E=Sophos;i="4.62,286,1297065600"; 9, 37 -- d="scan'208";a="453274563" 9, 37 -- X-Spam-Status: No 9, 37 -- X-Spam-Level: 9, 37 -- Received: from vapop.ucsd.edu ([132.239.84.98]) 9, 37 -- by iport-c2-out.ucsd.edu with ESMTP; 08 Mar 2011 13:45:56 -0800 9, 37 -- Received: from vandeberg by vapop.ucsd.edu (MDaemon PRO v9.6.6) 9, 37 -- with ESMTP id md50002066177.msg 9, 37 -- for {Microscopy-at-Microscopy.Com} ; Tue, 08 Mar 2011 13:44:33 -0800 9, 37 -- X-Spam-Processed: vapop.ucsd.edu, Tue, 08 Mar 2011 13:44:33 -0800 9, 37 -- (not processed: message from trusted or authenticated source) 9, 37 -- X-Return-Path: prvs=1048fe0e50=mmashore-at-vapop.ucsd.edu 9, 37 -- X-Envelope-From: mmashore-at-vapop.ucsd.edu 9, 37 -- X-MDaemon-Deliver-To: Microscopy-at-Microscopy.Com 9, 37 -- From: "Michael Mashore" {mmashore-at-vapop.ucsd.edu} 9, 37 -- To: {Microscopy-at-microscopy.com} 9, 37 -- Subject: Protocol for TEM of Cultured Cells 9, 37 -- Date: Tue, 8 Mar 2011 13:45:18 -0800 9, 37 -- Message-ID: {0794340FDC484A808EA515C6D79EA9C9-at-vandeberg} 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; 9, 37 -- charset="us-ascii" 9, 37 -- X-Priority: 3 (Normal) 9, 37 -- X-MSMail-Priority: Normal 9, 37 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 9, 37 -- Importance: Normal 9, 37 -- Thread-Index: Acvd2h2wgGkHrOWeSYq7PIowIEA3tw== 9, 37 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 9, 37 -- X-MDAV-Processed: vapop.ucsd.edu, Tue, 08 Mar 2011 13:44:34 -0800 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p28LkB3k016199 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 41 -- From jmircheski-at-us.es Thu Mar 10 03:44:58 2011 22, 41 -- Received: from mail.us.es (mail.us.es [193.147.175.20]) 22, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2A9iujn007699 22, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2011 03:44:57 -0600 22, 41 -- Received: (qmail 1683 invoked from network); 10 Mar 2011 10:44:55 +0100 22, 41 -- Received: from unknown (HELO us.es) (192.168.2.13) 22, 41 -- by us.es with SMTP; 10 Mar 2011 10:44:55 +0100 22, 41 -- Received: (qmail 26534 invoked by uid 507); 10 Mar 2011 09:44:54 -0000 22, 41 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on antivirus3 22, 41 -- X-Spam-Level: * 22, 41 -- X-Spam-Status: No, score=1.6 required=7.5 tests=BAYES_50,DOS_OUTLOOK_TO_MX, 22, 41 -- FSL_HELO_NON_FQDN_1,HELO_NO_DOMAIN autolearn=disabled version=3.3.1 22, 41 -- Received: from 127.0.0.1 by antivirus3 (envelope-from {jmircheski-at-us.es} , uid 501) with qmail-scanner-2.08 22, 41 -- (clamdscan: 0.97/12816. 22, 41 -- Clear:RC:1(127.0.0.1):. 22, 41 -- Processed in 0.048441 secs); 10 Mar 2011 09:44:54 -0000 22, 41 -- Received: from unknown (HELO antivirus3) (127.0.0.1) 22, 41 -- by us.es with SMTP; 10 Mar 2011 09:44:54 -0000 22, 41 -- Received: from 192.168.1.13 (192.168.1.13) 22, 41 -- by antivirus3 (F-Secure/fsigk_smtp/403/antivirus3); 22, 41 -- Thu, 10 Mar 2011 10:44:54 +0100 (CET) 22, 41 -- X-Virus-Status: clean(F-Secure/fsigk_smtp/403/antivirus3) 22, 41 -- Received: (qmail 14097 invoked from network); 10 Mar 2011 10:44:54 +0100 22, 41 -- Received: from macarfis.us.es (HELO cubiculos) (jmircheski-at-193.147.167.92) 22, 41 -- by us.es with RC4-MD5 encrypted SMTP; 10 Mar 2011 10:44:54 +0100 22, 41 -- Return-Receipt-To: "Josif Mircheski" {jmircheski-at-us.es} 22, 41 -- From: "Josif Mircheski" {jmircheski-at-us.es} 22, 41 -- To: {mmashore-at-vapop.ucsd.edu} , {Microscopy-at-microscopy.com} 22, 41 -- References: {201103082155.p28LtXO9031003-at-ns.microscopy.com} 22, 41 -- In-Reply-To: {201103082155.p28LtXO9031003-at-ns.microscopy.com} 22, 41 -- Subject: RE: [Microscopy] Protocol for TEM of Cultured Cells 22, 41 -- Date: Thu, 10 Mar 2011 10:45:41 +0100 22, 41 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAEPQoVbwH5VIu5W5KqvqHQfCgAAAEAAAAOpE9Mrk9+dMi9A2Q7uKZs0BAAAAAA==-at-us.es} 22, 41 -- MIME-Version: 1.0 22, 41 -- Content-Type: text/plain; 22, 41 -- charset="iso-8859-1" 22, 41 -- X-Mailer: Microsoft Office Outlook 12.0 22, 41 -- Thread-Index: Acvd25BgFWZBnFc9T22V4ETt+KnfQABJw4ZQ 22, 41 -- Content-Language: en-us 22, 41 -- Content-Transfer-Encoding: 8bit 22, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2A9iujn007699 ==============================End of - Headers==============================
Cultured cells can be challenging depending on whether the initial orientation is important and also because they tend to have relatively low contrast. A few suggestions are:
1) For preservation of original orientation: grow attached cells in plastic petri dishes. Do all fix, dehydrate, infiltrate right in the dish. Use ETOH to dehydrate but do not use propylene oxide. Just do your infiltration using ETOH for the graded steps and then embed in a very thin layer of resin. Cut the end off of gelatin capsules so they are a tube. Then just place them onto the polymerized cell/resin sheet, add 1 drop of resin, and again polymerize to secure them onto the cell/resin sheet. Then you can fill up the capsules and add the labels and polymerize for the third time. Alternatively, place the capsules on the resin sheet while it is still tacky so that the capsules will seal into the resin. Then fill with additional resin the next day and polymerize again. Break the sheet out of the petri dish and then cut out or break out the individual blocks with cells. Using liquid nitrogen will help release the cell sheet from the plastic petri dish. We prefer using petri dishes to the multi-well culture plates as they are easier to break.
2) If orientation is not important: Do fixation as desired. Scrape cells off of the culture dish, pellet, and enrobe in 1% agarose (see 4 below). Proceed with dehydration and infiltration of resulting blocks of cells and embed as normal.
3) Using reduced osmium really helps increase contrast in cultured cells. Reduced osmium: 1% OsO4 + 1.5% K3Fe(CN)6 [mix equal volumes of: 2% OsO4 in H2O + 3% K3Fe(CN)6]
4) It is best to limit centrifuging of cells in suspension as this can cause damage. We see this in bacteria when cells will have clear areas if fixed immediately after centrifugation. We avoid this by concentrating the cells using very gentle centrifugation or even letting them settle if this is an option. Then remove most of the media and resuspend cells. Let them sit for 10-15min to recover from the original centrifugation. Note that this may be a problem with some mammalian cells if they are not kept at the correct temperature and oxygen levels. In that case do not hold them but immediately proceed with fixation. Add fixative in 1:1 ratio to the concentrated cells and let them fix at appropriate temperature (usually 4oC for mammalian cells) for 5-10min. This will fix the cells nicely and there are no problems with fix not penetrating the pellet evenly. The you can spin, resuspend in fresh fix, and proceed. If the cells pellet well after initial fixation and pellet is small enough than you may not have to do any further spinning. If they do not stay together well or if pellet is large and you eventually want to embed it in multiple blocks then proceed as follows. Enrobe the cells with 1% low temperature gelling agarose (Sigma Type VII works well) after fixation to facilitate dehydration and embedding and remove need for further centrifugation. Dehydrate, infiltrate, and embed resulting blocks.
5) Cultured cells do really well with microwave-assisted fixation. If you have access to a scientific microwave designed for this purpose than by all means use it. It does a great job in both speeding up the process, minimizing extraction of soluble material from the cells, and just gives good overall preservation.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "jmircheski-at-us.es" {jmircheski-at-us.es} } Reply-To: "jmircheski-at-us.es" {jmircheski-at-us.es} } Date: Thu, 10 Mar 2011 04:47:07 -0500 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] RE: Protocol for TEM of Cultured Cells } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Michael, } } I am working at the moment with cells for standard EM. I have monolayer of } cells (these attach to glass goverslip) and a suspension of cells (which do } not attach to glass coverslip). I prepare them through a standard protocol } (glut/PFA, OsO4, uranyl acetate, dehydration, resin infiltration, resin } polymerisation). If I work with the monolayers, I do everything (up to the } resin) in a culture dish or a petri. For the suspension, I first pellet them } and then process in the same way as the others. Just trying to be extremely } careful not to disturb the pellet. In both cases, I try to exchange the } solutions very carefully and slowly. Both cell types are from mouse. } I too would like to hear/read what other people have to say on this. It } would be nice to hear other versions of the protocol and try to compare the } results. So, if there is anyone that has successfully implemented some } modifications in the protocol, please do share your experience. Michael, how } is the pre-embedding in Agar done and for type of cells? } } Regards, } } Josif } } (It's so nice to see that so many people on vacation at the moment! I guess } that my mailbox will be pleased to receive them all.) } } Dr. Josif Mircheski } ____________________________________________________________________________ } ___ } Instituto de Biomedicina de Sevilla (IBIS) } Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y } Dpto. Fisiologia Médica y Biofísica } Universidad de Sevilla } Facultad de Medicina } Avda. Sánchez-Pizjuán 4 } 41009-Sevilla } } Phone:+34-954556103 } Fax:+34-954551769 } e-mail: jmircheski-at-us.es } } -----Original Message----- } X-from: mmashore-at-vapop.ucsd.edu [mailto:mmashore-at-vapop.ucsd.edu] } Sent: Tuesday, March 08, 2011 10:56 PM } To: jmircheski-at-us.es } Subject: [Microscopy] Protocol for TEM of Cultured Cells } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } } I am looking for a working protocol preparing cells for standard } morphological TEM. So far I have read many different ways of doing this but } have never actually prepared cells for TEM. I was hoping to find out peoples } preferences, whether they fix cells in a monolayer, or a suspension, or just } fix the pellet. I was also wondering if people like to pre-embed in agar, } and what cell concentrations are preferred to start with. Any help would be } great. } } Thanks, } } Michael } } } } } } } ==============================Original Headers============================== } 9, 37 -- From prvs=1048fe0e50=mmashore-at-vapop.ucsd.edu Tue Mar 8 15:46:11 } 2011 } 9, 37 -- Received: from iport-c2-out.ucsd.edu (outbound.ucsd.edu } [132.239.0.119]) } 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p28LkB3k016199 } 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 8 Mar 2011 15:46:11 } -0600 } 9, 37 -- X-IronPort-Anti-Spam-Filtered: true } 9, 37 -- X-IronPort-Anti-Spam-Result: } AoMFAN4ydk2E71Ri/2dsb2JhbACJfI5+jW10t2KIa4VjBIUd } 9, 37 -- X-IronPort-AV: E=Sophos;i="4.62,286,1297065600"; } 9, 37 -- d="scan'208";a="453274563" } 9, 37 -- X-Spam-Status: No } 9, 37 -- X-Spam-Level: } 9, 37 -- Received: from vapop.ucsd.edu ([132.239.84.98]) } 9, 37 -- by iport-c2-out.ucsd.edu with ESMTP; 08 Mar 2011 13:45:56 -0800 } 9, 37 -- Received: from vandeberg by vapop.ucsd.edu (MDaemon PRO v9.6.6) } 9, 37 -- with ESMTP id md50002066177.msg } 9, 37 -- for {Microscopy-at-Microscopy.Com} ; Tue, 08 Mar 2011 13:44:33 } -0800 } 9, 37 -- X-Spam-Processed: vapop.ucsd.edu, Tue, 08 Mar 2011 13:44:33 -0800 } 9, 37 -- (not processed: message from trusted or authenticated } source) } 9, 37 -- X-Return-Path: prvs=1048fe0e50=mmashore-at-vapop.ucsd.edu } 9, 37 -- X-Envelope-From: mmashore-at-vapop.ucsd.edu } 9, 37 -- X-MDaemon-Deliver-To: Microscopy-at-Microscopy.Com } 9, 37 -- From: "Michael Mashore" {mmashore-at-vapop.ucsd.edu} } 9, 37 -- To: {Microscopy-at-microscopy.com} } 9, 37 -- Subject: Protocol for TEM of Cultured Cells } 9, 37 -- Date: Tue, 8 Mar 2011 13:45:18 -0800 } 9, 37 -- Message-ID: {0794340FDC484A808EA515C6D79EA9C9-at-vandeberg} } 9, 37 -- MIME-Version: 1.0 } 9, 37 -- Content-Type: text/plain; } 9, 37 -- charset="us-ascii" } 9, 37 -- X-Priority: 3 (Normal) } 9, 37 -- X-MSMail-Priority: Normal } 9, 37 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 9, 37 -- Importance: Normal } 9, 37 -- Thread-Index: Acvd2h2wgGkHrOWeSYq7PIowIEA3tw== } 9, 37 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 } 9, 37 -- X-MDAV-Processed: vapop.ucsd.edu, Tue, 08 Mar 2011 13:44:34 -0800 } 9, 37 -- Content-Transfer-Encoding: 8bit } 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p28LkB3k016199 } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 22, 41 -- From jmircheski-at-us.es Thu Mar 10 03:44:58 2011 } 22, 41 -- Received: from mail.us.es (mail.us.es [193.147.175.20]) } 22, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p2A9iujn007699 } 22, 41 -- for {Microscopy-at-microscopy.com} ; Thu, 10 Mar 2011 03:44:57 -0600 } 22, 41 -- Received: (qmail 1683 invoked from network); 10 Mar 2011 10:44:55 } +0100 } 22, 41 -- Received: from unknown (HELO us.es) (192.168.2.13) } 22, 41 -- by us.es with SMTP; 10 Mar 2011 10:44:55 +0100 } 22, 41 -- Received: (qmail 26534 invoked by uid 507); 10 Mar 2011 09:44:54 } -0000 } 22, 41 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on } antivirus3 } 22, 41 -- X-Spam-Level: * } 22, 41 -- X-Spam-Status: No, score=1.6 required=7.5 } tests=BAYES_50,DOS_OUTLOOK_TO_MX, } 22, 41 -- FSL_HELO_NON_FQDN_1,HELO_NO_DOMAIN autolearn=disabled version=3.3.1 } 22, 41 -- Received: from 127.0.0.1 by antivirus3 (envelope-from } {jmircheski-at-us.es} , uid 501) with qmail-scanner-2.08 } 22, 41 -- (clamdscan: 0.97/12816. } 22, 41 -- Clear:RC:1(127.0.0.1):. } 22, 41 -- Processed in 0.048441 secs); 10 Mar 2011 09:44:54 -0000 } 22, 41 -- Received: from unknown (HELO antivirus3) (127.0.0.1) } 22, 41 -- by us.es with SMTP; 10 Mar 2011 09:44:54 -0000 } 22, 41 -- Received: from 192.168.1.13 (192.168.1.13) } 22, 41 -- by antivirus3 (F-Secure/fsigk_smtp/403/antivirus3); } 22, 41 -- Thu, 10 Mar 2011 10:44:54 +0100 (CET) } 22, 41 -- X-Virus-Status: clean(F-Secure/fsigk_smtp/403/antivirus3) } 22, 41 -- Received: (qmail 14097 invoked from network); 10 Mar 2011 10:44:54 } +0100 } 22, 41 -- Received: from macarfis.us.es (HELO cubiculos) } (jmircheski-at-193.147.167.92) } 22, 41 -- by us.es with RC4-MD5 encrypted SMTP; 10 Mar 2011 10:44:54 +0100 } 22, 41 -- Return-Receipt-To: "Josif Mircheski" {jmircheski-at-us.es} } 22, 41 -- From: "Josif Mircheski" {jmircheski-at-us.es} } 22, 41 -- To: {mmashore-at-vapop.ucsd.edu} , {Microscopy-at-microscopy.com} } 22, 41 -- References: {201103082155.p28LtXO9031003-at-ns.microscopy.com} } 22, 41 -- In-Reply-To: {201103082155.p28LtXO9031003-at-ns.microscopy.com} } 22, 41 -- Subject: RE: [Microscopy] Protocol for TEM of Cultured Cells } 22, 41 -- Date: Thu, 10 Mar 2011 10:45:41 +0100 } 22, 41 -- Message-ID: } {!&!AAAAAAAAAAAYAAAAAAAAAEPQoVbwH5VIu5W5KqvqHQfCgAAAEAAAAOpE9Mrk9+dMi9A2Q7uKZs } 0BAAAAAA==-at-us.es} } 22, 41 -- MIME-Version: 1.0 } 22, 41 -- Content-Type: text/plain; } 22, 41 -- charset="iso-8859-1" } 22, 41 -- X-Mailer: Microsoft Office Outlook 12.0 } 22, 41 -- Thread-Index: Acvd25BgFWZBnFc9T22V4ETt+KnfQABJw4ZQ } 22, 41 -- Content-Language: en-us } 22, 41 -- Content-Transfer-Encoding: 8bit } 22, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p2A9iujn007699 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 31 -- From dsherman-at-purdue.edu Thu Mar 10 12:50:19 2011 11, 31 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2AIoIKr009356 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 10 Mar 2011 12:50:18 -0600 11, 31 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 11, 31 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p2AIoG8h009179; 11, 31 -- Thu, 10 Mar 2011 13:50:17 -0500 11, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB01F.purdue.lcl 11, 31 -- ([::1]) with mapi; Thu, 10 Mar 2011 13:50:17 -0500 11, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 31 -- To: "jmircheski-at-us.es" {jmircheski-at-us.es} , 11, 31 -- "message to: MSA list" 11, 31 -- {microscopy-at-microscopy.com} 11, 31 -- Date: Thu, 10 Mar 2011 13:50:14 -0500 11, 31 -- Subject: Re: [Microscopy] RE: Protocol for TEM of Cultured Cells 11, 31 -- Thread-Topic: [Microscopy] RE: Protocol for TEM of Cultured Cells 11, 31 -- Thread-Index: AcvfCB9rtzcLD7RhTBC2zVGpb1R4dwAS94qk 11, 31 -- Message-ID: {C99E8796.C3F2%dsherman-at-purdue.edu} 11, 31 -- In-Reply-To: {201103100947.p2A9l7R1010541-at-ns.microscopy.com} 11, 31 -- Accept-Language: en-US 11, 31 -- Content-Language: en-US 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 31 -- acceptlanguage: en-US 11, 31 -- Content-Type: text/plain; charset="iso-8859-1" 11, 31 -- MIME-Version: 1.0 11, 31 -- X-PMX-Version: 5.5.9.388399 11, 31 -- X-PerlMx-Virus-Scanned: Yes 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2AIoIKr009356 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Daisy.Ridings-at-carolinashealthcare.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Research Technician Position Opening
Message: Carolinas Medical Center in Charlotte, NC is seeking a full-time electron microscopy technician. The position involves clinical and research work. Duties include operation and basic maintenance of the transmission electron microscope, sample preparation, thick and thin sectioning, staining, and digital imaging.
Other required skills include the ability to work independently and interdependently as part of a high performance team and the ability to troubleshoot problems with techniques and equipment. Experience with digital imaging and Microsoft Office applications is highly desired. Immunocytochemistry experience is also helpful.
Bachelor's degree required. Master's degree and previous TEM experience preferred.
Applicants must apply online at http://careers.carolinashealthcare.org and search under Research positions.or at http://carolinashealthcare.jobscience.com/JsrApp/index.cfm?cmd=showPositionDetail&nextEvent=doSearchPositions&cobrandId=9000&masterId=chcs001&accountId=99F0AB63-0F40-BFD9-FB6461135C45CE9C&positionId=508788&urlArgList=c2VhcmNoVHlwZT1xdWljayZzdGFydD0xJmNvdW50PTUwJnBDb2RlPTExMDA5JmZhY2lsaXR5SWQ9JmpvYmR1cmF0aW9uSWQ9JnBvc3REYXlzPQ==&bid=1719 (direct link)
The Job ID# for this position is 208941-00013845KG.
Carolinas Medical Center is an Academic Medical Center Teaching Hospital and the flagship facility of Carolinas HealthCare System. Carolinas HealthCare System is an equal-opportunity not-for-profit, self-supporting public organization offering a wide variety of health and human services to residents of both North and South Carolina. Carolinas HealthCare System is the largest healthcare system in the Carolinas, and the third largest public system in the nation.
________________________________ This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you.
While I was working on the SEM, the HV was tripped at the beginning. When I turned the HV and filament current back on, the program I used indicated the HV and the filament were working properly. However, I could not see the beam anymore.
I am not sure if the HV tripping would actually cause any damage to the system. Any inputs on how to bring the beam back will be greatly appreciated.
Regards,
Sean EMC -at- SMU
==============================Original Headers============================== 9, 21 -- From xyang-at-SMU.CA Fri Mar 11 07:00:03 2011 9, 21 -- Received: from HUSKY0.SMU.CA (Husky0.smu.ca [140.184.1.100]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BD02R3029797 9, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 07:00:02 -0600 9, 21 -- Received: from s422XY ("port 4673"-at-[140.184.128.189]) 9, 21 -- by HUSKY1.SMU.CA (PMDF V6.3-x13 #31544) 9, 21 -- with ESMTP id {01NYS4S5CD448Y1QYN-at-HUSKY1.SMU.CA} for 9, 21 -- Microscopy-at-microscopy.com; Fri, 11 Mar 2011 09:00:02 -0400 9, 21 -- Date: Fri, 11 Mar 2011 09:00:02 -0400 9, 21 -- From: Xiang Yang {xyang-at-SMU.CA} 9, 21 -- Subject: how to diagnose e-beam disappearing problem 9, 21 -- To: Microscopy-at-microscopy.com 9, 21 -- Reply-to: xyang-at-SMU.CA 9, 21 -- Message-id: {007101cbdfec$3bfec7a0$b3fc56e0$-at-ca} 9, 21 -- MIME-version: 1.0 9, 21 -- X-Mailer: Microsoft Office Outlook 12.0 9, 21 -- Content-type: text/plain; charset=iso-8859-1 9, 21 -- Content-language: en-us 9, 21 -- Thread-Index: Acvf7DviTfZjqFI1TBW/EmFLdlW7+g== 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BD02R3029797 ==============================End of - Headers==============================
I think you're talking about a Zeiss sem with SmartSEM (or even LEO32) software, right?
If that is the case, the software is somewhat buggy and can get screwed up, especially when something like a HV trip happens. It could be as easy as the screen image is frozen (right click on your image display, choose 'commands' and 'normal' and that often unfreezes things). Sometimes it's more stuck than that and we sometimes need to reboot the PC. Occasionally it's even more stuck than that and rebooting the SEM does the trick (going to shutdown.. first to Yellow and then to Red, then bringing it all back up).
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
-----Original Message----- X-from: xyang-at-SMU.CA [mailto:xyang-at-SMU.CA] Sent: Friday, March 11, 2011 8:10 AM
Hi
First of all take a look at the scan system control to see if the beam has been blanked or frozen?
Then as you are using a computer driven instrument the next act that you must always carry out is to close down completely and reboot the system. You know what happens on your desk top PC if a program boots up wrong or crashes, well exactly the same happens with a PC driven microscope. Do not be satisfied with doing this once as I know a number of systems that need two or three reboots to get back to normality!
Does the system have a gun airlock which has closed and remained closed? It is no good taking it back to air and looking in the gun chamber because most systems with a gun airlock close it when air is admitted to any part of the system.
After that you may need the assistance of a service technician?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: xyang-at-SMU.CA [mailto:xyang-at-SMU.CA] Sent: 11 March 2011 13:01 To: protrain-at-emcourses.com
Hello everyone,
While I was working on the SEM, the HV was tripped at the beginning. When I turned the HV and filament current back on, the program I used indicated the HV and the filament were working properly. However, I could not see the beam anymore.
I am not sure if the HV tripping would actually cause any damage to the system. Any inputs on how to bring the beam back will be greatly appreciated.
Regards,
Sean EMC -at- SMU
==============================Original Headers============================== 9, 21 -- From xyang-at-SMU.CA Fri Mar 11 07:00:03 2011 9, 21 -- Received: from HUSKY0.SMU.CA (Husky0.smu.ca [140.184.1.100]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BD02R3029797 9, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 07:00:02 -0600 9, 21 -- Received: from s422XY ("port 4673"-at-[140.184.128.189]) 9, 21 -- by HUSKY1.SMU.CA (PMDF V6.3-x13 #31544) 9, 21 -- with ESMTP id {01NYS4S5CD448Y1QYN-at-HUSKY1.SMU.CA} for 9, 21 -- Microscopy-at-microscopy.com; Fri, 11 Mar 2011 09:00:02 -0400 9, 21 -- Date: Fri, 11 Mar 2011 09:00:02 -0400 9, 21 -- From: Xiang Yang {xyang-at-SMU.CA} 9, 21 -- Subject: how to diagnose e-beam disappearing problem 9, 21 -- To: Microscopy-at-microscopy.com 9, 21 -- Reply-to: xyang-at-SMU.CA 9, 21 -- Message-id: {007101cbdfec$3bfec7a0$b3fc56e0$-at-ca} 9, 21 -- MIME-version: 1.0 9, 21 -- X-Mailer: Microsoft Office Outlook 12.0 9, 21 -- Content-type: text/plain; charset=iso-8859-1 9, 21 -- Content-language: en-us 9, 21 -- Thread-Index: Acvf7DviTfZjqFI1TBW/EmFLdlW7+g== 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BD02R3029797 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 28 -- From protrain-at-emcourses.com Fri Mar 11 08:53:05 2011 26, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 26, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BEr4Va031764 26, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 08:53:04 -0600 26, 28 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:49240 helo=ProtrainPC) 26, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 26, 28 -- (envelope-from {protrain-at-emcourses.com} ) 26, 28 -- id 1Py3i8-000I5O-Od by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 11 Mar 2011 16:53:01 +0200 26, 28 -- Reply-To: {protrain-at-emcourses.com} 26, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 26, 28 -- To: {xyang-at-SMU.CA} 26, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 26, 28 -- References: {201103111300.p2BD0uH7031033-at-ns.microscopy.com} 26, 28 -- In-Reply-To: {201103111300.p2BD0uH7031033-at-ns.microscopy.com} 26, 28 -- Subject: RE: [Microscopy] how to diagnose e-beam disappearing problem 26, 28 -- Date: Fri, 11 Mar 2011 14:52:53 -0000 26, 28 -- Organization: Protrain 26, 28 -- Message-ID: {002f01cbdffc$01f88d10$05e9a730$-at-com} 26, 28 -- MIME-Version: 1.0 26, 28 -- Content-Type: text/plain; 26, 28 -- charset="iso-8859-1" 26, 28 -- X-Mailer: Microsoft Office Outlook 12.0 26, 28 -- Thread-Index: Acvf8Q7iOgLbaJyGRtO6RYf4wKCepgACWh3w 26, 28 -- Content-Language: en-gb 26, 28 -- X-Scan-Signature: 93ed830edcb21d82da58b9a49f2473dd{111}} 26, 28 -- X-Trace: smtp01.dial-up.net 1Py3i8-000I5O-Od d589982ba02fa115cc7da63648ccb77f 26, 28 -- Content-Transfer-Encoding: 8bit 26, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BEr4Va031764 ==============================End of - Headers==============================
Indeed it is a Leo 1450VP SEM. The computer and the SEM system has been reboot many times and the EDS system has also been turned off to eliminate any possibilities.
We have also double checked that the HV and filament were just fine.
Does it look like a lens problem or a gun tilt/shift problem?
Sean
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Friday, March 11, 2011 10:56 AM To: xyang-at-SMU.CA
Hi
First of all take a look at the scan system control to see if the beam has been blanked or frozen?
Then as you are using a computer driven instrument the next act that you must always carry out is to close down completely and reboot the system. You know what happens on your desk top PC if a program boots up wrong or crashes, well exactly the same happens with a PC driven microscope. Do not be satisfied with doing this once as I know a number of systems that need two or three reboots to get back to normality!
Does the system have a gun airlock which has closed and remained closed? It is no good taking it back to air and looking in the gun chamber because most systems with a gun airlock close it when air is admitted to any part of the system.
After that you may need the assistance of a service technician?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: xyang-at-SMU.CA [mailto:xyang-at-SMU.CA] Sent: 11 March 2011 13:01 To: protrain-at-emcourses.com
Hello everyone,
While I was working on the SEM, the HV was tripped at the beginning. When I turned the HV and filament current back on, the program I used indicated the HV and the filament were working properly. However, I could not see the beam anymore.
I am not sure if the HV tripping would actually cause any damage to the system. Any inputs on how to bring the beam back will be greatly appreciated.
Regards,
Sean EMC -at- SMU
==============================Original Headers============================== 9, 21 -- From xyang-at-SMU.CA Fri Mar 11 07:00:03 2011 9, 21 -- Received: from HUSKY0.SMU.CA (Husky0.smu.ca [140.184.1.100]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BD02R3029797 9, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 07:00:02 -0600 9, 21 -- Received: from s422XY ("port 4673"-at-[140.184.128.189]) 9, 21 -- by HUSKY1.SMU.CA (PMDF V6.3-x13 #31544) 9, 21 -- with ESMTP id {01NYS4S5CD448Y1QYN-at-HUSKY1.SMU.CA} for 9, 21 -- Microscopy-at-microscopy.com; Fri, 11 Mar 2011 09:00:02 -0400 9, 21 -- Date: Fri, 11 Mar 2011 09:00:02 -0400 9, 21 -- From: Xiang Yang {xyang-at-SMU.CA} 9, 21 -- Subject: how to diagnose e-beam disappearing problem 9, 21 -- To: Microscopy-at-microscopy.com 9, 21 -- Reply-to: xyang-at-SMU.CA 9, 21 -- Message-id: {007101cbdfec$3bfec7a0$b3fc56e0$-at-ca} 9, 21 -- MIME-version: 1.0 9, 21 -- X-Mailer: Microsoft Office Outlook 12.0 9, 21 -- Content-type: text/plain; charset=iso-8859-1 9, 21 -- Content-language: en-us 9, 21 -- Thread-Index: Acvf7DviTfZjqFI1TBW/EmFLdlW7+g== 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BD02R3029797 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 28 -- From protrain-at-emcourses.com Fri Mar 11 08:53:05 2011 26, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 26, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BEr4Va031764 26, 28 -- for {microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 08:53:04 -0600 26, 28 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:49240 helo=ProtrainPC) 26, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 26, 28 -- (envelope-from {protrain-at-emcourses.com} ) 26, 28 -- id 1Py3i8-000I5O-Od by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 11 Mar 2011 16:53:01 +0200 26, 28 -- Reply-To: {protrain-at-emcourses.com} 26, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 26, 28 -- To: {xyang-at-SMU.CA} 26, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 26, 28 -- References: {201103111300.p2BD0uH7031033-at-ns.microscopy.com} 26, 28 -- In-Reply-To: {201103111300.p2BD0uH7031033-at-ns.microscopy.com} 26, 28 -- Subject: RE: [Microscopy] how to diagnose e-beam disappearing problem 26, 28 -- Date: Fri, 11 Mar 2011 14:52:53 -0000 26, 28 -- Organization: Protrain 26, 28 -- Message-ID: {002f01cbdffc$01f88d10$05e9a730$-at-com} 26, 28 -- MIME-Version: 1.0 26, 28 -- Content-Type: text/plain; 26, 28 -- charset="iso-8859-1" 26, 28 -- X-Mailer: Microsoft Office Outlook 12.0 26, 28 -- Thread-Index: Acvf8Q7iOgLbaJyGRtO6RYf4wKCepgACWh3w 26, 28 -- Content-Language: en-gb 26, 28 -- X-Scan-Signature: 93ed830edcb21d82da58b9a49f2473dd{111}} 26, 28 -- X-Trace: smtp01.dial-up.net 1Py3i8-000I5O-Od d589982ba02fa115cc7da63648ccb77f 26, 28 -- Content-Transfer-Encoding: 8bit 26, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BEr4Va031764 ==============================End of - Headers==============================
==============================Original Headers============================== 38, 23 -- From xyang-at-SMU.CA Fri Mar 11 12:06:06 2011 38, 23 -- Received: from HUSKY8.SMU.CA (Husky8.smu.ca [140.184.1.8]) 38, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BI66bk000703 38, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 12:06:06 -0600 38, 23 -- Received: from s422XY ("port 1717"-at-[140.184.128.189]) 38, 23 -- by HUSKY1.SMU.CA (PMDF V6.3-x13 #31544) 38, 23 -- with ESMTP id {01NYSFGM0JYI8Y0E05-at-HUSKY1.SMU.CA} for 38, 23 -- Microscopy-at-microscopy.com; Fri, 11 Mar 2011 14:06:05 -0400 38, 23 -- Date: Fri, 11 Mar 2011 14:06:05 -0400 38, 23 -- From: Xiang Yang {xyang-at-SMU.CA} 38, 23 -- Subject: [Microscopy] RE: how to diagnose e-beam disappearing problem 38, 23 -- In-reply-to: {201103111455.p2BEtrOj003306-at-ns.microscopy.com} 38, 23 -- To: Microscopy-at-microscopy.com 38, 23 -- Reply-to: xyang-at-SMU.CA 38, 23 -- Message-id: {000801cbe016$fd2fc620$f78f5260$-at-ca} 38, 23 -- MIME-version: 1.0 38, 23 -- X-Mailer: Microsoft Office Outlook 12.0 38, 23 -- Content-type: text/plain; charset=iso-8859-1 38, 23 -- Content-language: en-us 38, 23 -- Thread-Index: Acvf/GuWuHBhQ6nbR0ahYv2twdMU6QAGcwJQ 38, 23 -- References: {201103111455.p2BEtrOj003306-at-ns.microscopy.com} 38, 23 -- Content-Transfer-Encoding: 8bit 38, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BI66bk000703 ==============================End of - Headers==============================
Hi, microscopists, We are trying to figure out what is wrong with our Zeiss TEM. The vacuum reads 5 x 10-3 milliBar and goes no further down on the gauge on the console.
We have checked the seals on the gun chamber and camera chamber and they seem to be o.k. We checked the forepumps by disconnecting the vacuum lines from the column, and both are pulling over 30 lbs/sqin. Diffusion pump heater is going on, and the cooling water is cooling the outside. The Penning gauge was cleaned and that didn't change the reading. We think the main valve is opening because the gauge on the console only reads the column vacuum. We are running out of ideas. Perhaps there is something obvious that is preventing the column from pumping down. Ideas, anyone? Carol Heckman Bowling Green State University
==============================Original Headers============================== 2, 30 -- From heckman-at-bgsu.edu Fri Mar 11 16:51:11 2011 2, 30 -- Received: from msa03.bgsu.edu (msa03.bgsu.edu [129.1.16.154]) 2, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BMpBrG028901 2, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 16:51:11 -0600 2, 30 -- X-AuditID: 8101109a-b7b50ae000003a75-91-4d7aa760f4e1 2, 30 -- Received: from mail.bgsu.edu (exhub.bgsu.edu [129.1.16.13]) 2, 30 -- (using TLS with cipher RC4-MD5 (RC4-MD5/128 bits)) 2, 30 -- (Client did not present a certificate) 2, 30 -- by msa03.bgsu.edu (BGSU Mail Gateway) with SMTP id 1C.63.14965.067AA7D4; Fri, 11 Mar 2011 17:51:12 -0500 (EST) 2, 30 -- Received: from MAIL4.bgsu.edu ([129.1.16.22]) by exhub03.bgsu.edu 2, 30 -- ([129.1.16.13]) with mapi; Fri, 11 Mar 2011 17:51:10 -0500 2, 30 -- From: Carol Heckman {heckman-at-bgsu.edu} 2, 30 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} 2, 30 -- Date: Fri, 11 Mar 2011 17:51:10 -0500 2, 30 -- Subject: FW: troubleshooting Zeiss EM 10 2, 30 -- Thread-Topic: troubleshooting Zeiss EM 10 2, 30 -- Thread-Index: AcvgGdbstr2LgMV6SmmZq+NgPB+8+wAJAXjz 2, 30 -- Message-ID: {E76D862B01CC7845BF120D9591635FA012774DFBE1-at-MAIL4.bgsu.edu} 2, 30 -- References: {201103111826.p2BIQS9n020086-at-ns.microscopy.com} 2, 30 -- In-Reply-To: {201103111826.p2BIQS9n020086-at-ns.microscopy.com} 2, 30 -- Accept-Language: en-US 2, 30 -- Content-Language: en-US 2, 30 -- X-MS-Has-Attach: 2, 30 -- X-MS-TNEF-Correlator: 2, 30 -- acceptlanguage: en-US 2, 30 -- Content-Type: text/plain; charset="us-ascii" 2, 30 -- MIME-Version: 1.0 2, 30 -- X-Brightmail-Tracker: AAAAAA== 2, 30 -- Content-Transfer-Encoding: 8bit 2, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BMpBrG028901 ==============================End of - Headers==============================
Carol, I once had a leaky aperture changer, but only leaking in one of the three possible positions. Found it only by chance... ;-) Did you check if the pressure gets down when you are changing positions on one of the three aperture changers? Other possible points of interest: - all movable parts (plate camera, viewing screen, specimen movements...) - what happens when you set the plate camera to atmospheric pressure? Fastest solution for a fix: get a leak tester
Best wishes, Stefan
------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D-97072 Wuerzburg Germany Tel. +49-931-7848700 Fax +49-931-7848701 Mobile +49-175-7177051
Am 11.03.2011 um 23:57 schrieb heckman-at-bgsu.edu:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, microscopists, } We are trying to figure out what is wrong with our Zeiss TEM. The vacuum reads 5 x 10-3 milliBar and goes no further down on the gauge on } the console. } } We have checked the seals on the gun chamber and camera chamber and they seem to be o.k. } We checked the forepumps by disconnecting the vacuum lines from the column, and both are pulling over 30 lbs/sqin. } Diffusion pump heater is going on, and the cooling water is cooling the outside. } The Penning gauge was cleaned and that didn't change the reading. } We think the main valve is opening because the gauge on the console only reads the column vacuum. } We are running out of ideas. Perhaps there is something obvious that is preventing the column from pumping down. } Ideas, anyone? } Carol Heckman } Bowling Green State University } } ==============================Original Headers============================== } 2, 30 -- From heckman-at-bgsu.edu Fri Mar 11 16:51:11 2011 } 2, 30 -- Received: from msa03.bgsu.edu (msa03.bgsu.edu [129.1.16.154]) } 2, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BMpBrG028901 } 2, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 16:51:11 -0600 } 2, 30 -- X-AuditID: 8101109a-b7b50ae000003a75-91-4d7aa760f4e1 } 2, 30 -- Received: from mail.bgsu.edu (exhub.bgsu.edu [129.1.16.13]) } 2, 30 -- (using TLS with cipher RC4-MD5 (RC4-MD5/128 bits)) } 2, 30 -- (Client did not present a certificate) } 2, 30 -- by msa03.bgsu.edu (BGSU Mail Gateway) with SMTP id 1C.63.14965.067AA7D4; Fri, 11 Mar 2011 17:51:12 -0500 (EST) } 2, 30 -- Received: from MAIL4.bgsu.edu ([129.1.16.22]) by exhub03.bgsu.edu } 2, 30 -- ([129.1.16.13]) with mapi; Fri, 11 Mar 2011 17:51:10 -0500 } 2, 30 -- From: Carol Heckman {heckman-at-bgsu.edu} } 2, 30 -- To: "MicroscopyListserver (E-mail)" {Microscopy-at-microscopy.com} } 2, 30 -- Date: Fri, 11 Mar 2011 17:51:10 -0500 } 2, 30 -- Subject: FW: troubleshooting Zeiss EM 10 } 2, 30 -- Thread-Topic: troubleshooting Zeiss EM 10 } 2, 30 -- Thread-Index: AcvgGdbstr2LgMV6SmmZq+NgPB+8+wAJAXjz } 2, 30 -- Message-ID: {E76D862B01CC7845BF120D9591635FA012774DFBE1-at-MAIL4.bgsu.edu} } 2, 30 -- References: {201103111826.p2BIQS9n020086-at-ns.microscopy.com} } 2, 30 -- In-Reply-To: {201103111826.p2BIQS9n020086-at-ns.microscopy.com} } 2, 30 -- Accept-Language: en-US } 2, 30 -- Content-Language: en-US } 2, 30 -- X-MS-Has-Attach: } 2, 30 -- X-MS-TNEF-Correlator: } 2, 30 -- acceptlanguage: en-US } 2, 30 -- Content-Type: text/plain; charset="us-ascii" } 2, 30 -- MIME-Version: 1.0 } 2, 30 -- X-Brightmail-Tracker: AAAAAA== } 2, 30 -- Content-Transfer-Encoding: 8bit } 2, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BMpBrG028901 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 25 -- From stefan.diller-at-t-online.de Fri Mar 11 17:13:19 2011 7, 25 -- Received: from mailout11.t-online.de (mailout11.t-online.de [194.25.134.85]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2BNDIhO012744 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Mar 2011 17:13:19 -0600 7, 25 -- Received: from fwd05.aul.t-online.de (fwd05.aul.t-online.de ) 7, 25 -- by mailout11.t-online.de with smtp 7, 25 -- id 1PyBWH-000474-2j; Sat, 12 Mar 2011 00:13:17 +0100 7, 25 -- Received: from [192.168.2.239] (SUn-n0ZOrhs+5n5WeRxGj3ULlGKL1-ukFideYfMBer9ouXYtwUjGnmp8CVxC7tdga5-at-[87.178.229.124]) by fwd05.t-online.de 7, 25 -- with esmtp id 1PyBWG-0DVJvE0; Sat, 12 Mar 2011 00:13:16 +0100 7, 25 -- References: {201103112257.p2BMvXhm002739-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {201103112257.p2BMvXhm002739-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 (iPhone Mail 8C148) 7, 25 -- Content-Type: text/plain; 7, 25 -- charset=us-ascii 7, 25 -- Message-Id: {4F6A8E6C-DCB8-410E-A1F7-B1F188F242C4-at-t-online.de} 7, 25 -- X-Mailer: iPhone Mail (8C148) 7, 25 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 25 -- Subject: Re: [Microscopy] FW: troubleshooting Zeiss EM 10 7, 25 -- Date: Sat, 12 Mar 2011 00:13:14 +0100 7, 25 -- To: "heckman-at-bgsu.edu" {heckman-at-bgsu.edu} , 7, 25 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 25 -- X-ID: SUn-n0ZOrhs+5n5WeRxGj3ULlGKL1-ukFideYfMBer9ouXYtwUjGnmp8CVxC7tdga5 7, 25 -- X-TOI-MSGID: 0e1772a1-fcee-40ff-8c6f-90189e37deb7 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2BNDIhO012744 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jwoperei-at-email.smith.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I am wondering how microscope equipment housed in a non- BSL2 facility is being cleaned after being used for BSL2 research. Both our Leica SP5 confocal microscope w/incubation chamber and OlympusBX51 light microscope are being used for this project. Any information about what disinfectant to use, what parts to clean and how this is done is welcome. Maybe you have a microscope cleaning protocol in place and want to share that with me.
Any information about this is welcome. Best, Judith Wopereis.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] GLP Certification of EM facility
Message: Hi,
I was asked if it were possible to GLP certify our EM operations. Is there anyone out there that has GLP certified their EM facility or that works in a GLP certified facility that could help me?
Thank you, Angel Paredes Director EM services NCTR Jefferson, Arkansas
A postdoctoral position in Cryo-EM and structure analysis is available at the University of Texas Medical Branch at Galveston to study the structure and active conformation of membrane-associated protein and complexes involved in blood coagulation: http://www.utmb.edu/ncb/faculty/Stoilova-McPhieSvetla.asp. Highly motivated candidates with expertise in macromolecular structure analysis, modeling and biophysics are encouraged to apply. Additional experience with Linux computing systems will be an advantage. The Sealy Center for Structural Biology and Molecular Biophysics at UTMB, as well as the PI’s lab are fully equipped for state of the art structure determination of macromolecular complexes: http://www.scsb.utmb.edu/. UTMB is part of the Gulf Coast Consortium: http://cohesion.rice.edu/centersandinst/gcc/ and the Texas Medical Center, which houses the NIH funded National center for Macromolecular Imaging: http://ncmi.bcm.edu/ncmi/. Interested candidates should send a CV including areas of expertise and interest, publications list, and names and contact information for three references to svmcphie-at-utmb.edu.
Svetla Stoilova-McPhie, PhD Assistant Professor, Department of Neuroscience and Cell Biology Scientist, Sealy Centre for Structural Biology and Molecular Biophysics University of Texas Medical Branch at Galveston 301 University Boulevard, Galveston, Texas 77555-0620 Lab: (+1) 409-747-2159 Cell: (+1) 979-319-1348 Fax: (1+) 409-747-2200 Email: svmcphie-at-utmb.edu http://www.utmb.edu/ncb/faculty/Stoilova-McPhieSvetla.asp
==============================Original Headers============================== 3, 31 -- From svmcphie-at-utmb.edu Mon Mar 14 00:07:08 2011 3, 31 -- Received: from mx3.utmb.edu (mx3.utmb.edu [129.109.195.8]) 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2E577N6002522 3, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 00:07:07 -0500 3, 31 -- Received: from expf1.utmb.edu (expf1.utmb.edu [129.109.116.226]) 3, 31 -- by mx3.utmb.edu (8.13.8/8.13.8) with ESMTP id p2E576f4019799 3, 31 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NO) 3, 31 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 00:07:07 -0500 3, 31 -- Received: from EXCHANGE4.utmb.edu ([129.109.116.11]) by expf1.utmb.edu 3, 31 -- ([129.109.116.226]) with mapi; Mon, 14 Mar 2011 00:07:06 -0500 3, 31 -- From: "Stoilova-McPhie, Svetla" {svmcphie-at-utmb.edu} 3, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 31 -- Date: Mon, 14 Mar 2011 00:07:05 -0500 3, 31 -- Subject: Postdoctoral Position in Cryo-EM and structure determination 3, 31 -- Thread-Topic: Postdoctoral Position in Cryo-EM and structure determination 3, 31 -- Thread-Index: AQHL4gWpRGqjoABIy0yWmII9Uzl+gQ== 3, 31 -- Message-ID: {981C42C3AD3C6D489252BB66CD90374521099D7BDB-at-EXCHANGE4.utmb.edu} 3, 31 -- Accept-Language: en-US 3, 31 -- Content-Language: en-US 3, 31 -- X-MS-Has-Attach: 3, 31 -- X-MS-TNEF-Correlator: 3, 31 -- acceptlanguage: en-US 3, 31 -- Content-Type: text/plain; charset="Windows-1252" 3, 31 -- MIME-Version: 1.0 3, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 3, 31 -- definitions=2011-03-14_01:2011-03-11,2011-03-14,1970-01-01 signatures=0 3, 31 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 3, 31 -- phishscore=0 bulkscore=1 adultscore=0 classifier=spam adjust=0 reason=mlx 3, 31 -- scancount=1 engine=6.0.2-1012030000 definitions=main-1103130176 3, 31 -- Content-Transfer-Encoding: 8bit 3, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2E577N6002522 ==============================End of - Headers==============================
I'm sure most of you have seen the articles and images, but still, not bad images for a cell phone. Maybe cell was the right adjective for these radio phones...
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 6, 15 -- From frank_karl-at-lincolnelectric.com Mon Mar 14 05:56:28 2011 6, 15 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 6, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2EAuSOX027754 6, 15 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 05:56:28 -0500 6, 15 -- Subject: cross polar images with your cell phone 6, 15 -- To: Microscopy-at-microscopy.com 6, 15 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 6, 15 -- Message-ID: {OF020BCDD0.9BA04208-ON85257853.003BCDD2-85257853.003C198E-at-lincolnelectric.com} 6, 15 -- From: Frank_Karl-at-lincolnelectric.com 6, 15 -- Date: Mon, 14 Mar 2011 06:56:26 -0400 6, 15 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 6, 15 -- 03/14/2011 06:56:27 AM 6, 15 -- MIME-Version: 1.0 6, 15 -- Content-Type: text/plain; 6, 15 -- charset="US-ASCII" ==============================End of - Headers==============================
We have been asked to microtome salt crystals. The crystals will be on the order of 120nm and sections need to be under 5µm. I assume that we will have to embed the crystals and then try to make ~2µm sections using glass knives. I am concerned that the crystals might damage diamond knives.
I thought to use Spurrs resin but do not know if we will actually get infiltration sufficient to keep the crystals from falling out. Sections will have to be cut dry since water would dissolve the crystal.
Any suggestions as to type of embedding resin would be appreciated or experience in microtoming similar samples.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 9, 28 -- From dsherman-at-purdue.edu Mon Mar 14 19:55:26 2011 9, 28 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2F0tQd6001940 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 19:55:26 -0500 9, 28 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) 9, 28 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p2F0tPo7011717 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 20:55:26 -0400 9, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB03H.purdue.lcl 9, 28 -- ([172.21.6.92]) with mapi; Mon, 14 Mar 2011 20:55:25 -0400 9, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 9, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 9, 28 -- Date: Mon, 14 Mar 2011 20:55:24 -0400 9, 28 -- Subject: Microtome salt crystals 9, 28 -- Thread-Topic: Microtome salt crystals 9, 28 -- Thread-Index: Acviq6oGeaifKwuoikCVlcfLcMOOcA== 9, 28 -- Message-ID: {C9A4313B.C933%dsherman-at-purdue.edu} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="iso-8859-1" 9, 28 -- MIME-Version: 1.0 9, 28 -- X-PMX-Version: 5.5.9.388399 9, 28 -- X-PerlMx-Virus-Scanned: Yes 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2F0tQd6001940 ==============================End of - Headers==============================
Is there a typo in your message? I assume you mean the crystals are ~120um and you want 2um thick particles. A 2um thick crystal is too thick for EM, so I assume this is for LM.
Why do you need to microtome? Could you possibly crush the salt crystals and dust the dry powder on a thin carbon or lacy film? A mortar and pestle should break up most stuff into the size range you want. You may be able to get even finer by crushing/grinding between between glass microscope slides.
I would be surprised if rock salt damaged a diamond knife. It isn't very hard.
Cheers, Henk
At 3/14/2011 8:56 PM, dsherman-at-purdue.edu wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } We have been asked to microtome salt crystals. The crystals will be on the } order of 120nm and sections need to be under 5µm. I assume that we will } have to embed the crystals and then try to make ~2µm sections using glass } knives. I am concerned that the crystals might damage diamond knives. } } I thought to use Spurrs resin but do not know if we will actually get } infiltration sufficient to keep the crystals from falling out. Sections } will have to be cut dry since water would dissolve the crystal. } } Any suggestions as to type of embedding resin would be appreciated or } experience in microtoming similar samples. } } Debby } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail:dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy } } } } } ==============================Original Headers============================== } 9, 28 -- Fromdsherman-at-purdue.edu Mon Mar 14 19:55:26 2011 } 9, 28 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2F0tQd6001940 } 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 19:55:26 -0500 } 9, 28 -- Received: from WPPEXHUB03H.purdue.lcl (wppexhub03h.itap.purdue.edu [172.21.6.92]) } 9, 28 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p2F0tPo7011717 } 9, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 20:55:26 -0400 } 9, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB03H.purdue.lcl } 9, 28 -- ([172.21.6.92]) with mapi; Mon, 14 Mar 2011 20:55:25 -0400 } 9, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 9, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 9, 28 -- Date: Mon, 14 Mar 2011 20:55:24 -0400 } 9, 28 -- Subject: Microtome salt crystals } 9, 28 -- Thread-Topic: Microtome salt crystals } 9, 28 -- Thread-Index: Acviq6oGeaifKwuoikCVlcfLcMOOcA== } 9, 28 -- Message-ID: {C9A4313B.C933%dsherman-at-purdue.edu} } 9, 28 -- Accept-Language: en-US } 9, 28 -- Content-Language: en-US } 9, 28 -- X-MS-Has-Attach: } 9, 28 -- X-MS-TNEF-Correlator: } 9, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 9, 28 -- acceptlanguage: en-US } 9, 28 -- Content-Type: text/plain; charset="iso-8859-1" } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- X-PMX-Version: 5.5.9.388399 } 9, 28 -- X-PerlMx-Virus-Scanned: Yes } 9, 28 -- Content-Transfer-Encoding: 8bit } 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2F0tQd6001940 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 9, 27 -- From colijn.1-at-osu.edu Mon Mar 14 20:21:51 2011 9, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2F1LoFX018361 9, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 20:21:51 -0500 9, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 27 -- id {01NYX1JUZBXC9G44ER-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 9, 27 -- Mon, 14 Mar 2011 21:21:48 -0400 (EDT) 9, 27 -- Received: from [192.168.1.104] 9, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 9, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 27 -- with ESMTPA id {01NYX1JT29409EQCEL-at-ecr6.ohio-state.edu} for 9, 27 -- microscopy-at-microscopy.com; Mon, 14 Mar 2011 21:21:46 -0400 (EDT) 9, 27 -- Date: Mon, 14 Mar 2011 21:21:45 -0400 9, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 27 -- Subject: Re: [Microscopy] Microtome salt crystals 9, 27 -- In-reply-to: {201103150056.p2F0usei003893-at-ns.microscopy.com} 9, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 27 -- To: microscopy-at-microscopy.com 9, 27 -- Message-id: {4D7EBF29.5040903-at-osu.edu} 9, 27 -- MIME-version: 1.0 9, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 27 -- Content-transfer-encoding: 8BIT 9, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.15) 9, 27 -- Gecko/20110303 Thunderbird/3.1.9 9, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 27 -- References: {201103150056.p2F0usei003893-at-ns.microscopy.com} ==============================End of - Headers==============================
No the original was correct. These are microcrystals. This is for a chemistry lab that works on methods to determine crystallinity in proteins and other materials. They are doing this using second harmonic generation (SHG) properties in microcrystals. I do not know the details but the hope is that SHG microscopy can be used to speed up crystal detection in protein crystal structure determination.
Crushing the crystals would damage the symmetry they hope to retain.
Debby
On 3/14/11 9:15 PM, "Hendrik Colijn" {colijn.1-at-OSU.edu} wrote:
} Hi Debby, } } Is there a typo in your message? I assume you mean the crystals are ~120um } and you want 2um thick particles. A 2um thick crystal is too thick for EM, so } I assume this is for LM. } } Why do you need to microtome? Could you possibly crush the salt crystals and } dust the dry powder on a thin carbon or lacy film? A mortar and pestle should } break up most stuff into the size range you want. You may be able to get even } finer by crushing/grinding between between glass microscope slides. } } I would be surprised if rock salt damaged a diamond knife. It isn't very } hard. } } Cheers, } Henk
==============================Original Headers============================== 8, 28 -- From dsherman-at-purdue.edu Mon Mar 14 20:33:03 2011 8, 28 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2F1X3f1002047 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 20:33:03 -0500 8, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 8, 28 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p2F1X3RL002673 8, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Mar 2011 21:33:03 -0400 8, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.1.40]) by WPPEXHUB02F.purdue.lcl 8, 28 -- ([::1]) with mapi; Mon, 14 Mar 2011 21:33:03 -0400 8, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 8, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 8, 28 -- Date: Mon, 14 Mar 2011 21:33:02 -0400 8, 28 -- Subject: Re: Microtome salt crystals 8, 28 -- Thread-Topic: Microtome salt crystals 8, 28 -- Thread-Index: AcvisOvlzespcvhrNUSAKdPVegBlag== 8, 28 -- Message-ID: {C9A43A0D.C947%dsherman-at-purdue.edu} 8, 28 -- Accept-Language: en-US 8, 28 -- Content-Language: en-US 8, 28 -- X-MS-Has-Attach: 8, 28 -- X-MS-TNEF-Correlator: 8, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 8, 28 -- acceptlanguage: en-US 8, 28 -- Content-Type: text/plain; charset="us-ascii" 8, 28 -- MIME-Version: 1.0 8, 28 -- X-PMX-Version: 5.5.9.388399 8, 28 -- X-PerlMx-Virus-Scanned: Yes 8, 28 -- Content-Transfer-Encoding: 8bit 8, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2F1X3f1002047 ==============================End of - Headers==============================
Actually your question is strange because BSL2 research should be performed in a BSL2 facility!! I don't think that a standard procedure would be the best. The procedure certainly depends on the kind of BSL2 you are facing. Are these bacteria, viruses, human cells, parasites?
Best regards,
Stephane
----- Original Message ---- X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Sat, March 12, 2011 3:43:11 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jwoperei-at-email.smith.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I am wondering how microscope equipment housed in a non- BSL2 facility is being cleaned after being used for BSL2 research. Both our Leica SP5 confocal microscope w/incubation chamber and OlympusBX51 light microscope are being used for this project. Any information about what disinfectant to use, what parts to clean and how this is done is welcome. Maybe you have a microscope cleaning protocol in place and want to share that with me.
Any information about this is welcome. Best, Judith Wopereis.
Any cleaning protocol should be part if the risk assessment done before starting work and it has to be tailored to the specific organism you're looking at. If you're not in a level two area though the specimen should almost certainly be in some way deactivated before looking at it. We routinely hand class 2 and worse organisms but they're dead before we start on them - usually crosslinking with glutaraldehyde is sufficient. If you have to look at them live then a sealed slide should be the way to go making decontamination unnecessary.
Most things that could be used to decontaminate are not going to do delicate optics much good - our usual reagents are sodium hypochlorite,2m sodium hydroxide ( for very tough things ) and formalin vapour ( which requires a room that can be fumigated ), I wouldn't like to use anything other than 70% ethanol on a 'scope. Cheers Ian
----- Original Message ---- X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Sat, March 12, 2011 3:43:11 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bwilson-at-salud.unm.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bwilson-at-salud.unm.edu Name: Bridget Wilson
Organization: University of New Mexico
Title-Subject: [Filtered] EM technician/staff scientist opening
Message: A full-time position is available for an electron microscopy technician/staff scientist to join a busy, multi-faculty laboratory group at the University of New Mexico in scenic New Mexico. Funding for the group comes from multiple institutes at the NIH, as well as NSF and the Human Frontiers Science Program. This interdisciplinary group integrates electron and fluorescence imaging with biochemistry and with mathematical modeling to obtain a more complete understanding of the spatiotemporal events that initiate signaling across cell membranes. The group has developed novel “rip-flip” technologies to study the topography of receptors and associated signaling molecules on the landscape of the plasma membrane. The applicant will be trained in this innovative technique. The applicant will also be trained in image analysis procedures developed within the laboratory group and develop new nanoparticle probes. Fluorescence microscopy techniques will also be applied, to screen antibodies and perform correlative studies related to the EM projects. We will consider both entry-level and experienced applicants. The successful applicant will already be familiar with conventional electron microscopy techniques, including embedding, thin-sectioning, staining and TEM imaging. Additional familiarity with the basics of mammalian cell culture and fluorescence microscopy techniques (immunofluorescence, confocal imaging) would be helpful. Good computer skills and good record keeping, trouble shooting, self-motivation and interpersonal skills are important criteria. This is a great opportunity to live in the beautiful Southwest! Contact Bridget S. Wilson, PhD Send CV, statement of interest and references to: bwilson-at-salud.unm.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both reynolds-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: reynolds-at-vt.edu Name: Bill Reynolds
Organization: Virginia Tech
Title-Subject: [Filtered] Job Opening: Nanoscale Characterization and Fabrication Lab / Virginia Tech
Message: The Nanoscale Characterization and Fabrication Laboratory at Virginia Tech announces an opening for a focussed-ion beam specialist. The open position is for a Research Associate or Senior Research Associate, "Nanoscale Instrumentation Specialist", and the required qualifications include: working knowledge of analytical instrumentation, scanning electron microscopy, and focused ion-beam operating principles; experience operating and maintaining these and/or related instruments; a willingness to help out where needed with other equipment. Experience with nanoindentation, scanned probe microscopy, TEM, SIMS, XPS, or Auger is a plus. Experience with instrument troubleshooting and maintenance is desirable. The successful candidate will be in charge of an FEI Helios Nanolab 600 Focused Ion Beam (FIB). Depending upon qualifications, the candidate can be appointed as a research faculty. Information about the facility is available at: http://www.ictas.vt.edu/facilities/ncfl Applications will be accepted on-line at: http://www.hr.vt.edu/employment/ Interested candidates should apply to Posting Number 0110095.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both j.janssen-at-nki.nl as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: j.janssen-at-nki.nl Name: Hans Janssen
Organization: Netherlands Cancer Institute
Title-Subject: [Filtered] protocols for TEM of cultured cells
Message: Josif and Michael, I like to add 3 extra possibilities to the mail of Debbie. There are commercially available plastic cover slips called Thermanox (in different shapes and sizes). You can grow your cells on it and use the standard embedding procedures. Also available are small petri dishes with a foil as bottom, in here you grow your cells follow the standard procedure iup to 100% ethanol then cut out a piece of foil and continu the embedding with it. A drawback is that while sectioning the sections will split on the plastic layer, so your cells will be at the edge of the sections. The sections can have a wavy appearence. The third is an oldfashion method. Coat a glass cover slip with a relatively thick layer of carbon, grow your cells on the carbon, follow the standard embedding procedure but use for the epon a flat mold that exactly fits the glass cover slip. This to prevent epon from coming to the bottom side. After polymerisation you can peel of the glass, it will split on the carbon layer. Then re-embed to get epon on the carbon side. I have no commercial interest in the mentioned products. Good luck, Hans janssen.
Josif and Michael,
Cultured cells can be challenging depending on whether the initial orientation is important and also because they tend to have relatively low contrast. A few suggestions are:
1) For preservation of original orientation: grow attached cells in plastic petri dishes. Do all fix, dehydrate, infiltrate right in the dish. Use ETOH to dehydrate but do not use propylene oxide. Just do your infiltration using ETOH for the graded steps and then embed in a very thin layer of resin. Cut the end off of gelatin capsules so they are a tube. Then just place them onto the polymerized cell/resin sheet, add 1 drop of resin, and again polymerize to secure them onto the cell/resin sheet. Then you can fill up the capsules and add the labels and polymerize for the third time. Alternatively, place the capsules on the resin sheet while it is still tacky so that the capsules will seal into the resin. Then fill with additional resin the next day and polymerize again. Break the sheet out of the petri dish and then cut out or break out the individual blocks with cells. Using liquid nitrogen will help release the cell sheet from the plastic petri dish. We prefer using petri dishes to the multi-well culture plates as they are easier to break.
2) If orientation is not important: Do fixation as desired. Scrape cells off of the culture dish, pellet, and enrobe in 1% agarose (see 4 below). Proceed with dehydration and infiltration of resulting blocks of cells and embed as normal.
3) Using reduced osmium really helps increase contrast in cultured cells. Reduced osmium: 1% OsO4 + 1.5% K3Fe(CN)6 [mix equal volumes of: 2% OsO4 in H2O + 3% K3Fe(CN)6] 4) It is best to limit centrifuging of cells in suspension as this can cause damage. We see this in bacteria when cells will have clear areas if fixed immediately after centrifugation. We avoid this by concentrating the cells using very gentle centrifugation or even letting them settle if this is an option. Then remove most of the media and resuspend cells. Let them sit for 10-15min to recover from the original centrifugation. Note that this may be a problem with some mammalian cells if they are not kept at the correct temperature and oxygen levels. In that case do not hold them but immediately proceed with fixation. Add fixative in 1:1 ratio to the concentrated cells and let them fix at appropriate temperature (usually 4oC for mammalian cells) for 5-10min. This will fix the cells nicely and there are no problems with fix not penetrating the pellet evenly. The you can spin, resuspend in fresh fix, and proceed. If the cells pellet well after initial fixation and pellet is small enough than you may not have to do any further spinning. If they do not stay together well or if pellet is large and you eventually want to embed it in multiple blocks then proceed as follows. Enrobe the cells with 1% low temperature gelling agarose (Sigma Type VII works well) after fixation to facilitate dehydration and embedding and remove need for further centrifugation. Dehydrate, infiltrate, and embed resulting blocks.
5) Cultured cells do really well with microwave-assisted fixation. If you have access to a scientific microwave designed for this purpose than by all means use it. It does a great job in both speeding up the process, minimizing extraction of soluble material from the cells, and just gives good overall preservation.
Debby
==============================Original Headers============================== 13, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Mar 16 18:44:50 2011 13, 28 -- Received: from mx0b-000f2801.pphosted.com (mx0b-000f2801.pphosted.com [67.231.152.119]) 13, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2GNiowf028793 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Mar 2011 18:44:50 -0500 13, 28 -- Received: from pps.filterd (m0000739 [127.0.0.1]) 13, 28 -- by mx0b-000f2801.pphosted.com (8.14.3/8.14.3) with SMTP id p2GNeMCf020026 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Mar 2011 19:44:49 -0400 13, 28 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) 13, 28 -- by mx0b-000f2801.pphosted.com with ESMTP id v2u3eft7b-1 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Mar 2011 19:44:49 -0400 13, 28 -- Received: from NestorPwrBk.local ([192.168.14.119]) 13, 28 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p2GNinZc089189 13, 28 -- for {microscopy-at-microscopy.com} ; Wed, 16 Mar 2011 23:44:49 GMT 13, 28 -- Message-ID: {4D814B70.4030109-at-microscopy.com} 13, 28 -- Date: Wed, 16 Mar 2011 18:44:48 -0500 13, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 28 -- Reply-To: j.janssen-at-nki.nl 13, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 13, 28 -- MIME-Version: 1.0 13, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 28 -- Subject: viaWWW:protocols for TEM of cultured cells 13, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 28 -- Content-Transfer-Encoding: 7bit 13, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 13, 28 -- definitions=2011-03-16_03:2011-03-16,2011-03-16,1970-01-01 signatures=0 13, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 13, 28 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 13, 28 -- engine=6.0.2-1012030000 definitions=main-1103160164 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both james.m.hoffmann-at-usa.dupont.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: james.m.hoffmann-at-usa.dupont.com Name: Jim Hoffmann
Organization: E.I. Dupont
Title-Subject: [Filtered] SEM - Table Top SEM versus Research Grade SEM
Message: Hello Listserver Folks,
I am seeking your advice and experience. I am an Optical Microscopist working at a manufacturing site in a busy microscopy lab. The type of samples that I see cover a wide range including: failure analysis, customer support, small particle identification, R&D support, manufacturing issues, materials analysis, defect identification, root cause troubleshooting, Quality Control, polymers, organics, inorganics, etc... Besides Optical microscopy I also use Micro FTIR spectroscopy. I would like to add an SEM to my lab primarily so that I can get EDS elemental information to complement the FTIR information. The increased magnification and depth of field beyond optical microscopy would be an added bonus but not the critical need. I usually don't need over 1000x, but occasionally I need to go to 10,000 - 20,000x. For this I send samples out for SEM. I was wondering if any of you folks have insights or experience with desk-top SEM's such as EVEX Mini-SEM, Aspex Express/Explorer, or Hitachi TM3000 ? Since I am not a trained SEM microscopist, I am thinking that the table-top models would be just right for me ... easier to operate with less training ... easier maintenance ... no sputter coating required ... What would I be giving up in terms of EDS capability and Imaging capability? Also, since I am at a manufacturing facility, funds are tight in my lab. I am thinking I may need to go with a "previously owned" instrument... I am not sure how much I have to spend at this point ....
We have a busy lab with a number of microscopes (optical, SEM, TEM etc). While most of our SEMs are full size systems (Zeiss, JEOL), some with EDS, we also have a tabletop Hitachi TM1000 SEM. I also recently had a TM3000 with EDS in as a demo unit to try out and compare. (it's handy to have a small fast SEM right in the lab area).
Most of our work is semiconductor based.
First off, we are impressed with the TM1000 (and even more impressed with the TM3000) in how quickly and easily it can be used to get great images (albeit limited in mag). It definitely has limited controls (compared to a full size system) but you can learn how to use it in about 30 seconds. It is much harder for people to screw it up also! The EDS was quick and intuitive to learn how to use and had fast mapping capabilities. EDS capability was similar to what we have on our full size SEM (by no means 'dumbed down').
Pros: Fast, easy to use (for trained and non-trained people alike). Excellent quality images. Various 'high pressure' modes meaning you often don't need to coat. No special services required (water, special power, air -plug it in to standard outlet!) and small size (really is table-top). Easy to exchange filaments
Cons: Limited magnification BSE detector only (no SE) Limited beam voltages (have choice of a few fixed steps) Small stage
Pricing was a bit hard to pin down.. base SEM was ~$80k, add another ~$75k for EDS. Other options like motorized stage are available. We were looking at the demo unit (year end sale) and price was about 25% discount.
I have seen TM1000s on the used market for ~$10k or less with no EDS.
Definitely 'try before you buy' - and one thing I'm still not sure about is the stated 'magnification'. We have our SEMS mostly set to use Polaroid output as the reference for mag but I'm not sure what Hitachi uses. To get the same image, 10,000x on the Hitachi = 3,000x on our Zeiss and JEOL.. confusing!
Finally, our TM1000 has been very reliable except for the turbo.. it only lasted a year or so before making bad noises. Hitachi replaced it no problems but I don't know if this is a problem area or not. The TM3000 uses a new turbo.
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
Email: james.m.hoffmann-at-usa.dupont.com Name: Jim Hoffmann
Organization: E.I. Dupont
Title-Subject: [Filtered] SEM - Table Top SEM versus Research Grade SEM
Message: Hello Listserver Folks,
I am seeking your advice and experience. I am an Optical Microscopist working at a manufacturing site in a busy microscopy lab. The type of samples that I see cover a wide range including: failure analysis, customer support, small particle identification, R&D support, manufacturing issues, materials analysis, defect identification, root cause troubleshooting, Quality Control, polymers, organics, inorganics, etc... Besides Optical microscopy I also use Micro FTIR spectroscopy. I would like to add an SEM to my lab primarily so that I can get EDS elemental information to complement the FTIR information. The increased magnification and depth of field beyond optical microscopy would be an added bonus but not the critical need. I usually don't need over 1000x, but occasionally I need to go to 10,000 - 20,000x. For this I send samples out for SEM. I was wondering if any of you folks have insights or experience with desk-top SEM's such as EVEX Mini-SEM, Aspex Express/Explorer, or Hitachi TM3000 ? Since I am not a trained SEM microscopist, I am thinking that the table-top models would be just right for me ... easier to operate with less training ... easier maintenance ... no sputter coating required ... What would I be giving up in terms of EDS capability and Imaging capability? Also, since I am at a manufacturing facility, funds are tight in my lab. I am thinking I may need to go with a "previously owned" instrument... I am not sure how much I have to spend at this point ....
CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation.
==============================Original Headers============================== 23, 27 -- From cpawlowicz-at-ubmtechinsights.com Thu Mar 17 08:18:02 2011 23, 27 -- Received: from pegasus.semiconductor.com (semi-fw.ubmtechinsights.com [207.61.67.10]) 23, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2HDI1Id011580 23, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 08:18:01 -0500 23, 27 -- Received: from pegasus.semiconductor.com ([192.168.2.10]) by pegasus 23, 27 -- ([192.168.2.10]) with mapi; Thu, 17 Mar 2011 09:17:29 -0400 23, 27 -- From: Chris Pawlowicz {cpawlowicz-at-ubmtechinsights.com} 23, 27 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 23, 27 -- CC: "'james.m.hoffmann-at-usa.dupont.com'" {james.m.hoffmann-at-usa.dupont.com} 23, 27 -- Date: Thu, 17 Mar 2011 09:17:28 -0400 23, 27 -- Subject: RE: [Microscopy] viaWWW:SEM - Table Top SEM versus Research Grade 23, 27 -- SEM 23, 27 -- Thread-Topic: [Microscopy] viaWWW:SEM - Table Top SEM versus Research Grade 23, 27 -- SEM 23, 27 -- Thread-Index: AcvkNfCxPllp39yhQwayVvBwf/2rNwAad+2A 23, 27 -- Message-ID: {0079587FD46DFC42AC07C9D337C7C895395FD0ED5B-at-pegasus} 23, 27 -- References: {201103162358.p2GNwCVx024150-at-ns.microscopy.com} 23, 27 -- In-Reply-To: {201103162358.p2GNwCVx024150-at-ns.microscopy.com} 23, 27 -- Accept-Language: en-US 23, 27 -- Content-Language: en-US 23, 27 -- X-MS-Has-Attach: 23, 27 -- X-MS-TNEF-Correlator: 23, 27 -- acceptlanguage: en-US 23, 27 -- Content-Type: text/plain; charset="us-ascii" 23, 27 -- MIME-Version: 1.0 23, 27 -- Content-Transfer-Encoding: 8bit 23, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2HDI1Id011580 ==============================End of - Headers==============================
Does anyone out there have an extra owners manual they can send to me for an LKB NOVA ultramicrotme?
Fred Hayes Manager Interdisciplinary Center for Electron Microscopy (ICEM) / Kemper Hall Facility, room 0108 Det of Chemical Engineering and Material Sciences 3118 Bainer Hall Univ of CA Davis Davis CA 95616 530-752-0284 office
==============================Original Headers============================== 5, 24 -- From fahayes-at-ucdavis.edu Thu Mar 17 13:05:02 2011 5, 24 -- Received: from smtp1.ucdavis.edu (smtp1.ucdavis.edu [128.120.32.218]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2HI50W7001685 5, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 13:05:01 -0500 5, 24 -- Received: from chmsfred ([169.237.230.150]) 5, 24 -- by smtp1.ucdavis.edu (8.13.1/8.13.1/it-rhel5-mimedefang-smtp-1.1) with ESMTP id p2HI4wKm057698 5, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 11:04:59 -0700 5, 24 -- From: "Fred Hayes" {fahayes-at-ucdavis.edu} 5, 24 -- To: {Microscopy-at-microscopy.com} 5, 24 -- Subject: need an owners manual for LKB NOVA 5, 24 -- Date: Thu, 17 Mar 2011 11:04:58 -0700 5, 24 -- Message-ID: {003501cbe4cd$d4724bc0$7d56e340$-at-edu} 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Type: text/plain; 5, 24 -- charset="us-ascii" 5, 24 -- Content-Transfer-Encoding: 7bit 5, 24 -- X-Mailer: Microsoft Office Outlook 12.0 5, 24 -- Thread-Index: AcvkzdIzDyBfsP6LTYONw8ySb5DEuA== 5, 24 -- Content-Language: en-us 5, 24 -- x-cr-hashedpuzzle: CHz8 CTsA CdGV CpRY DDhQ EedN Eypz F0fC Gp9k JVpu JW8r Jm0y KAdU KK18 Lpq/ MH9G;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{2BEADDDE-059C-49A7-8FC7-7A914361699C};ZgBhAGgAYQB5AGUAcwBAAHUAYwBkAGEAdgBpAHMALgBlAGQAdQA=;Thu, 17 Mar 2011 18:04:55 GMT;bgBlAGUAZAAgAGEAbgAgAG8AdwBuAGUAcgBzACAAbQBhAG4AdQBhAGwAIABmAG8AcgAgAEwASwBCACAATgBPAFYAQQA= 5, 24 -- x-cr-puzzleid: {2BEADDDE-059C-49A7-8FC7-7A914361699C} 5, 24 -- X-Virus-Scanned: clamav-milter 0.97 at av5 5, 24 -- X-Virus-Status: Clean 5, 24 -- X-Scanned-By: MIMEDefang 2.67 on 128.120.32.8 ==============================End of - Headers==============================
Dear colleagues, (appologies to anyone also on the sx-50 or MSA list)
I am currently developing a collection of standards for a new electron microprobe lab at Concord University in West Virginia, USA. I have gratefully received samples from the Smithsonian and Harvard along with several USGS, MPI-DING, and NIST glasses. I also have several more samples generously provided by a number of people. An array of purchased minerals, metals, and simple compounds should arrive during the coming weeks. Even still, there remain some gaps.
For example, I am looking for synthetic Ni and Co-olivines. I am aware of some grown in Japan decades ago, but I have been unable to locate a current source.
Silicates with significant amounts of Sr, Rb, or V also have proven difficult to find.
I am also aware of some synthetic Cl-apatite grown in France (Argiolas and Baumer, Can. Min., v. 16, pp 285-290, 1978) but have been unable to track down any material.
Suggestions of other useful materials or offers of the same also would be much appreciated.
In exchange for any samples that may be provided, I can offer one, although somewhat indirectly. I have been characterizing a homogeneous rhyolitic obsidian from the collections of the Harvard Mineralogical Museum - sample ID3506 from Lipari Is., Italy. Major/minor elements are well-characterized by several methods including a 25-lab EPMA intercomparison (manuscript in review). Some trace/REE data are available as well (XRF, ICP-AES, ICP-MS, LA-ICP-MS). Harvard has the material for distribution. I can provide compositional data on request.
The Concord University probe lab itself is a bit unusual in several respects. It's the only probe that we're aware of in West Virginia (if anyone knows of another, please let me know). It is also located at a 2800-student, mainly undergraduate state university. Although the lab is new, it is largely based on older equipment. We have a refurbished ARL-SEMQ that was previously located at the University of Kentucky. We have modern software in the form of Probe for EPMA, and we recently obtained funding to upgrade to a new SDD EDS system. The key focus for the lab will be undergraduate teaching and research, but time will be available for outside commercial and academic users. We also plan to develop the capability for remote operation of the key analytical functions. Next week, we will have a poster at the Southeastern Section meeting of the Geological Society of America. If you are at the meeting, stop by and say hello.
Thanks and best regards,
- Steve Kuehn
--
---------------- Dr. Stephen C. Kuehn Research Scientist and Lecturer Manager, Electron Microprobe Facility Division of Natural Sciences Science, room 106
Concord University, Campus Box F20 1000 Vermillion St PO Box 1000 Athens, WV 24712-1000
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hwitkiewicz-at-prism-sd.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Automated EM Immunogold Labeling
Message: Hello everybody,
I wonder if any of you have any experience with or opinion about the Leica EM IGL, sold by Marine Reef International http://www.marinereef.com/emigl.php.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both walter.bobrowski-at-pfizer.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: walter.bobrowski-at-pfizer.com Name: Walt Bobrowski
Organization: MSA
Title-Subject: [Filtered] LR White ISH Troubleshooting
Message: Intranuclear RNA ISH on formalin-fixed paraffin embedded working fine. Attempts to perform same on paraformaldehyde-fixed, LR White embedded samples at the light level meet with no success. Liver samples fixed in 4% PF, dehydrated through 95% EtOH, and heat cured (55C) in LR White. Attempting ISH on 2um thick sections. All references indicate this should work with no problem. Have tried protease and Triton-x pretreatments of varying concentrations and times. Any and all trouble-shooting tips would be welcome (including section preparation steps to avoid).
Also welcome are tips to get 1.5-2um thick sections to lay flat on glass slides without heat application. Thanks in advance! Walt
Hello Jim, I don't have experience with table top SEMs, but your job sounds like what I did at Goodyear, or at least the equipment sounds the same. I agree you don't want a research grade SEM, but I think you will find you need more than a table top.
I found I used both secondary electron (detailed pictures) and backscatter electron (helps me find the areas with different average atomic number for EDS) as well as EDS. I found some plastics charged so bad I needed to gold or carbon coat, and I found the Pd/Pt interfered the least with the elements I was interested in. You should consider a sputter.
An environmental SEM, with provisions for reduced vacuum (and reduced charging) in the sample chamber, proved so useful for looking at difficult to coat samples like carbon black pellets, glassy spheres on conductive tape and so many other specimens, but you can only use backscatter because problems with light caused by an interaction of low pressure gas and electrons.
I can't tell you how many times I've used different acceleration KVs I want 30 KV for S and Mo detection. Surface details on paint, plastic or the insect built in to your product might call for 4kv. I like 25kv for steel but 15kv for thinish films.
Of course not all your specimens will be orientated in the chamber for best imaging or EDS, so the more rotation and tilt you have the better. Don't forget the Z axis. Not all samples will be the same size and I'll forsaken EDS data to increase the Z axis to get lower magnification images. I still wish I could get 10x magnification on some samples. The ability to have different apertures to change the depth of focus has proven invaluable. It's not always about high magnification, sometime you want the peaks and valleys of an irregular surface in focus.
Your experience will be different, but anticipate that your uses in 5 years will not be the same as now. I would suggest a medium range SEM with mag up to 50k and a large chamber will serve you better in the long rum.
And don't forget about the room the scope is going in: low magnetic fields, low vibrations and constant current and voltage are a must to get the best out of your SEM.
Keep us informed.
Frank Karl Lincoln Electric
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 13, 16 -- From frank_karl-at-lincolnelectric.com Thu Mar 17 14:26:04 2011 13, 16 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2HJQ4lG002445 13, 16 -- for {microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 14:26:04 -0500 13, 16 -- Subject: SEM: to table top or not, that is the question 13, 16 -- Sensitivity: 13, 16 -- To: Microscopy-at-microscopy.com, james.m.hoffmann-at-usa.dupont.com 13, 16 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 13, 16 -- Message-ID: {OF6419792A.E4D39524-ON85257856.006A738B-85257856.006AC06C-at-lincolnelectric.com} 13, 16 -- From: Frank_Karl-at-lincolnelectric.com 13, 16 -- Date: Thu, 17 Mar 2011 15:26:00 -0400 13, 16 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 13, 16 -- 03/17/2011 03:25:59 PM 13, 16 -- MIME-Version: 1.0 13, 16 -- Content-Type: text/plain; 13, 16 -- charset="US-ASCII" ==============================End of - Headers==============================
Hi Debby, At one point I was trying to embed pine seeds, which also had a tendency to come out of the resin when we tried to section them. We adjusted the hardness of the resin to try to match the resin to the seeds, and we got one formulation that worked fairly well to retain the seeds in the sections. I would try this with your salt crystals. Good luck. Yours, Bill
X-from: dsherman-at-purdue.edu To: William.F.Tivol-at-aero.org
We have been asked to microtome salt crystals. The crystals will be on the order of 120nm and sections need to be under 5µm. I assume that we will have to embed the crystals and then try to make ~2µm sections using glass knives. I am concerned that the crystals might damage diamond knives.
I thought to use Spurrs resin but do not know if we will actually get infiltration sufficient to keep the crystals from falling out. Sections will have to be cut dry since water would dissolve the crystal.
Any suggestions as to type of embedding resin would be appreciated or experience in microtoming similar samples.
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2I2M5Bt012314 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 17 Mar 2011 21:22:05 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p2I2M5Hr012312; Thu, 17 Mar 2011 21:22:05 -0500
Hello all,
My trusty old Zeiss EM10CA TEM is having problems and I would like to know if anyone has spare parts, or a whole spare parts instrument, sitting around. Specifically I am in need of the "cascade" unit that sits on top of the high voltage tank. If you think you have a cascade and are willing to part with it I would like to hear from you.
For those curious about the symptoms or those wanting to help diagnose the problem:
The filament is good, the lenses have current, the column is under a good vacuum. With the high tension off there is no beam current. When I turn on the high tension without turning on the filament the beam current immediately goes to around 50uA and then creeps upwards to as high as 200uA. Therefore there is a leakage of current. When I turn on the filament, with or without the high tension, the current reading stays the same and a small circle of illumination shows on the phosphor screen. The circle is about 5mm in diameter and does not change with a change in Condenser 1 setting, mag setting or adjusting the beam alignments controls. It can be occluded using the retractable apertures.
The engineer has traced the problem to the cascade device.
Thanks, Louie
--
Louis Kerr lkerr-at-mbl.edu
Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
VISIT OUR WEB SITES: www.mbl.edu www.courses.mbl.edu
==============================Original Headers============================== 12, 32 -- From lkerr-at-mbl.edu Thu Mar 17 21:22:05 2011 12, 32 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2I2M5BE012309 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 21:22:05 -0500 12, 32 -- Received: from mailfrontier.mbl.edu (127.0.0.1) by mailfrontier.mbl.edu (MlfMTA v3.2r9) id hgb0sq0171sn for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:05 -0400 (envelope-from {lkerr-at-mbl.edu} ) 12, 32 -- Received: from mail.mbl.edu ([128.128.172.47]) 12, 32 -- by mailfrontier.mbl.edu (SonicWALL 7.2.1.2841) 12, 32 -- with ESMTP; Thu, 17 Mar 2011 22:22:05 -0400 12, 32 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 32 -- by mail.mbl.edu (Postfix) with ESMTP id 102BC1824001 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:05 -0400 (EDT) 12, 32 -- X-Virus-Scanned: amavisd-new at mbl.edu 12, 32 -- Received: from mail.mbl.edu ([127.0.0.1]) 12, 32 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10024) 12, 32 -- with ESMTP id 1caLm4FTL4CS for {Microscopy-at-microscopy.com} ; 12, 32 -- Thu, 17 Mar 2011 22:22:04 -0400 (EDT) 12, 32 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) 12, 32 -- by mail.mbl.edu (Postfix) with ESMTP id BA0C31730270 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:04 -0400 (EDT) 12, 32 -- Date: Thu, 17 Mar 2011 22:22:04 -0400 (EDT) 12, 32 -- From: Louis Kerr {lkerr-at-mbl.edu} 12, 32 -- To: Microscopy-at-microscopy.com 12, 32 -- Message-ID: {1782501564.26988.1300414924672.JavaMail.root-at-mail.mbl.edu} 12, 32 -- In-Reply-To: {1811288924.25189.1300391923960.JavaMail.root-at-mail.mbl.edu} 12, 32 -- Subject: Re: Zeiss EM10 TEM Parts 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Type: text/plain; charset=utf-8 12, 32 -- Content-Transfer-Encoding: 7bit 12, 32 -- X-Originating-IP: [76.119.200.182] 12, 32 -- X-Mailer: Zimbra 6.0.8_GA_2685 (ZimbraWebClient - FF3.0 (Mac)/6.0.8_GA_2685) 12, 32 -- X-Mlf-Version: 7.2.1.2841 12, 32 -- X-Mlf-UniqueId: o201103180222050052769 ==============================End of - Headers==============================
Hello Louie, this sounds to me more like a problem with a shortened high voltage cable, not the cascade. I have both the cascade and the cable. Contact me off-list, if you are interested.
Best regards, Stefan
------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D-97072 Wuerzburg Germany Tel. +49-931-7848700 Fax +49-931-7848701 Mobile +49-175-7177051
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } } My trusty old Zeiss EM10CA TEM is having problems and I would like to know if anyone has spare parts, or a whole spare parts instrument, sitting around. Specifically I am in need of the "cascade" unit that sits on top of the high voltage tank. If you think you have a cascade and are willing to part with it I would like to hear from you. } } } For those curious about the symptoms or those wanting to help diagnose the problem: } } The filament is good, the lenses have current, the column is under a good vacuum. With the high tension off there is no beam current. When I turn on the high tension without turning on the filament the beam current immediately goes to around 50uA and then creeps upwards to as high as 200uA. Therefore there is a leakage of current. When I turn on the filament, with or without the high tension, the current reading stays the same and a small circle of illumination shows on the phosphor screen. The circle is about 5mm in diameter and does not change with a change in Condenser 1 setting, mag setting or adjusting the beam alignments controls. It can be occluded using the retractable apertures. } } The engineer has traced the problem to the cascade device. } } Thanks, } Louie } } -- } } } Louis Kerr } lkerr-at-mbl.edu } } Research and Education Support Coordinator } Marine Biological Laboratory } 7 MBL Street } Woods Hole, MA 02543 } 508-289-7273 } 508-540-6902 (FAX) } 508-292-0289 (Cell phone) } } VISIT OUR WEB SITES: } www.mbl.edu } www.courses.mbl.edu } } ==============================Original Headers============================== } 12, 32 -- From lkerr-at-mbl.edu Thu Mar 17 21:22:05 2011 } 12, 32 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) } 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2I2M5BE012309 } 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 21:22:05 -0500 } 12, 32 -- Received: from mailfrontier.mbl.edu (127.0.0.1) by mailfrontier.mbl.edu (MlfMTA v3.2r9) id hgb0sq0171sn for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:05 -0400 (envelope-from {lkerr-at-mbl.edu} ) } 12, 32 -- Received: from mail.mbl.edu ([128.128.172.47]) } 12, 32 -- by mailfrontier.mbl.edu (SonicWALL 7.2.1.2841) } 12, 32 -- with ESMTP; Thu, 17 Mar 2011 22:22:05 -0400 } 12, 32 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 12, 32 -- by mail.mbl.edu (Postfix) with ESMTP id 102BC1824001 } 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:05 -0400 (EDT) } 12, 32 -- X-Virus-Scanned: amavisd-new at mbl.edu } 12, 32 -- Received: from mail.mbl.edu ([127.0.0.1]) } 12, 32 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10024) } 12, 32 -- with ESMTP id 1caLm4FTL4CS for {Microscopy-at-microscopy.com} ; } 12, 32 -- Thu, 17 Mar 2011 22:22:04 -0400 (EDT) } 12, 32 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) } 12, 32 -- by mail.mbl.edu (Postfix) with ESMTP id BA0C31730270 } 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:04 -0400 (EDT) } 12, 32 -- Date: Thu, 17 Mar 2011 22:22:04 -0400 (EDT) } 12, 32 -- From: Louis Kerr {lkerr-at-mbl.edu} } 12, 32 -- To: Microscopy-at-microscopy.com } 12, 32 -- Message-ID: {1782501564.26988.1300414924672.JavaMail.root-at-mail.mbl.edu} } 12, 32 -- In-Reply-To: {1811288924.25189.1300391923960.JavaMail.root-at-mail.mbl.edu} } 12, 32 -- Subject: Re: Zeiss EM10 TEM Parts } 12, 32 -- MIME-Version: 1.0 } 12, 32 -- Content-Type: text/plain; charset=utf-8 } 12, 32 -- Content-Transfer-Encoding: 7bit } 12, 32 -- X-Originating-IP: [76.119.200.182] } 12, 32 -- X-Mailer: Zimbra 6.0.8_GA_2685 (ZimbraWebClient - FF3.0 (Mac)/6.0.8_GA_2685) } 12, 32 -- X-Mlf-Version: 7.2.1.2841 } 12, 32 -- X-Mlf-UniqueId: o201103180222050052769 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 25 -- From stefan.diller-at-t-online.de Fri Mar 18 01:19:06 2011 7, 25 -- Received: from mailout02.t-online.de (mailout02.t-online.de [194.25.134.17]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2I6J6jS000897 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 18 Mar 2011 01:19:06 -0500 7, 25 -- Received: from fwd02.aul.t-online.de (fwd02.aul.t-online.de ) 7, 25 -- by mailout02.t-online.de with smtp 7, 25 -- id 1Q0T1i-00005s-6s; Fri, 18 Mar 2011 07:19:10 +0100 7, 25 -- Received: from [10.174.19.157] (XYSHEeZBQhLUYC-71C2xxFSyzH0Vbpy58otjRFGgg5hL5dTBwdbViSp42WkkoG6g8+-at-[80.187.101.125]) by fwd02.t-online.de 7, 25 -- with esmtp id 1Q0T1W-1avCpU0; Fri, 18 Mar 2011 07:18:58 +0100 7, 25 -- References: {201103180226.p2I2QwYS018509-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {201103180226.p2I2QwYS018509-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 (iPhone Mail 8C148) 7, 25 -- Content-Type: text/plain; 7, 25 -- charset=us-ascii 7, 25 -- Message-Id: {E31689C8-98D6-4C76-B134-6F3B8669D3EB-at-t-online.de} 7, 25 -- Cc: microscopy-at-microscopy.com 7, 25 -- X-Mailer: iPhone Mail (8C148) 7, 25 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 25 -- Subject: Re: [Microscopy] Re: Zeiss EM10 TEM Parts 7, 25 -- Date: Fri, 18 Mar 2011 07:18:57 +0100 7, 25 -- To: "lkerr-at-mbl.edu" {lkerr-at-mbl.edu} 7, 25 -- X-ID: XYSHEeZBQhLUYC-71C2xxFSyzH0Vbpy58otjRFGgg5hL5dTBwdbViSp42WkkoG6g8+ 7, 25 -- X-TOI-MSGID: 7d4ae670-cb04-4edd-a977-6eae73701d46 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2I6J6jS000897 ==============================End of - Headers==============================
I see your email with interest, particularly your "bright spot"!
Firstly the problem you outline is due to the high voltage generator or the cable? A simple test is to disconnect the high voltage cable from the tank and repeat your actions. If there is a change in current it suggests that it is the cable that is the problem? If this is the case a local high voltage company, working with x-ray sets for example, should be able to refurbish it for you at a fraction of what the original equipment manufacturers charge.
However back to your "bright spot"! What you are seeing is the illumination generated by the filament being heated; you are seeing "light" from the tip rather than electrons! This phenomenon has always been around and has confused engineers/technicians re aligning and instrument after a strip down for decades. Having found what they think is the beam they are loathe to lose it and that is often what is required to find the true "electron beam".
Good luck with the high voltage problem
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: lkerr-at-mbl.edu [mailto:lkerr-at-mbl.edu] Sent: 18 March 2011 02:23 To: protrain-at-emcourses.com
Hello all,
My trusty old Zeiss EM10CA TEM is having problems and I would like to know if anyone has spare parts, or a whole spare parts instrument, sitting around. Specifically I am in need of the "cascade" unit that sits on top of the high voltage tank. If you think you have a cascade and are willing to part with it I would like to hear from you.
For those curious about the symptoms or those wanting to help diagnose the problem:
The filament is good, the lenses have current, the column is under a good vacuum. With the high tension off there is no beam current. When I turn on the high tension without turning on the filament the beam current immediately goes to around 50uA and then creeps upwards to as high as 200uA. Therefore there is a leakage of current. When I turn on the filament, with or without the high tension, the current reading stays the same and a small circle of illumination shows on the phosphor screen. The circle is about 5mm in diameter and does not change with a change in Condenser 1 setting, mag setting or adjusting the beam alignments controls. It can be occluded using the retractable apertures.
The engineer has traced the problem to the cascade device.
Thanks, Louie
--
Louis Kerr lkerr-at-mbl.edu
Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
VISIT OUR WEB SITES: www.mbl.edu www.courses.mbl.edu
==============================Original Headers============================== 12, 32 -- From lkerr-at-mbl.edu Thu Mar 17 21:22:05 2011 12, 32 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2I2M5BE012309 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 21:22:05 -0500 12, 32 -- Received: from mailfrontier.mbl.edu (127.0.0.1) by mailfrontier.mbl.edu (MlfMTA v3.2r9) id hgb0sq0171sn for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:05 -0400 (envelope-from {lkerr-at-mbl.edu} ) 12, 32 -- Received: from mail.mbl.edu ([128.128.172.47]) 12, 32 -- by mailfrontier.mbl.edu (SonicWALL 7.2.1.2841) 12, 32 -- with ESMTP; Thu, 17 Mar 2011 22:22:05 -0400 12, 32 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 12, 32 -- by mail.mbl.edu (Postfix) with ESMTP id 102BC1824001 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:05 -0400 (EDT) 12, 32 -- X-Virus-Scanned: amavisd-new at mbl.edu 12, 32 -- Received: from mail.mbl.edu ([127.0.0.1]) 12, 32 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10024) 12, 32 -- with ESMTP id 1caLm4FTL4CS for {Microscopy-at-microscopy.com} ; 12, 32 -- Thu, 17 Mar 2011 22:22:04 -0400 (EDT) 12, 32 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) 12, 32 -- by mail.mbl.edu (Postfix) with ESMTP id BA0C31730270 12, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 22:22:04 -0400 (EDT) 12, 32 -- Date: Thu, 17 Mar 2011 22:22:04 -0400 (EDT) 12, 32 -- From: Louis Kerr {lkerr-at-mbl.edu} 12, 32 -- To: Microscopy-at-microscopy.com 12, 32 -- Message-ID: {1782501564.26988.1300414924672.JavaMail.root-at-mail.mbl.edu} 12, 32 -- In-Reply-To: {1811288924.25189.1300391923960.JavaMail.root-at-mail.mbl.edu} 12, 32 -- Subject: Re: Zeiss EM10 TEM Parts 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Type: text/plain; charset=utf-8 12, 32 -- Content-Transfer-Encoding: 7bit 12, 32 -- X-Originating-IP: [76.119.200.182] 12, 32 -- X-Mailer: Zimbra 6.0.8_GA_2685 (ZimbraWebClient - FF3.0 (Mac)/6.0.8_GA_2685) 12, 32 -- X-Mlf-Version: 7.2.1.2841 12, 32 -- X-Mlf-UniqueId: o201103180222050052769 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 27 -- From protrain-at-emcourses.com Fri Mar 18 04:48:24 2011 27, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 27, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2I9mMof021618 27, 27 -- for {microscopy-at-microscopy.com} ; Fri, 18 Mar 2011 04:48:23 -0500 27, 27 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:49317 helo=ProtrainPC) 27, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 27, 27 -- (envelope-from {protrain-at-emcourses.com} ) 27, 27 -- id 1Q0WI7-000I3r-Fs by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 18 Mar 2011 11:48:20 +0200 27, 27 -- Reply-To: {protrain-at-emcourses.com} 27, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 27, 27 -- To: {lkerr-at-mbl.edu} 27, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 27, 27 -- References: {201103180223.p2I2NJAi013552-at-ns.microscopy.com} 27, 27 -- In-Reply-To: {201103180223.p2I2NJAi013552-at-ns.microscopy.com} 27, 27 -- Subject: RE: [Microscopy] Re: Zeiss EM10 TEM Parts 27, 27 -- Date: Fri, 18 Mar 2011 09:47:52 -0000 27, 27 -- Organization: Protrain 27, 27 -- Message-ID: {003b01cbe551$9bd59a90$d380cfb0$-at-com} 27, 27 -- MIME-Version: 1.0 27, 27 -- Content-Type: text/plain; 27, 27 -- charset="us-ascii" 27, 27 -- Content-Transfer-Encoding: 7bit 27, 27 -- X-Mailer: Microsoft Office Outlook 12.0 27, 27 -- Thread-Index: AcvlE4BPf7Y+tsUJT5aY/VLXvYq4GgAOx3wQ 27, 27 -- Content-Language: en-gb 27, 27 -- X-Scan-Signature: a4aa4773d5e53c4992d0e3c7dead50ca{139}} 27, 27 -- X-Trace: smtp01.dial-up.net 1Q0WI7-000I3r-Fs d04ec04360d36a416e1a14c46304cb37 ==============================End of - Headers==============================
Watching the replies I think I sit in the middle between the comments generated so far.
Only last week I was using a "table top" with clients and I am always amazed at how good they are and with EDS they make a great tool. However I often have difficulty with the limited range of accelerating voltages.
There is always an accelerating voltage that displays the information from a specimen better than any other. At the same time there is often a considerable difference in what is visualised by the table top backscattered electron image and an Everhart-Thornley image. True, we see less charge with backscatter as used in the table top instruments, but there are times when a secondary image is the only one that will suffice!
Then there is another problem that moving to backscatter does not remove charge, the effect is just minimised. However if you want EDS quantification you cannot afford to have any charge as this will spoil the results. The EDS computing system is unable to account for the reduction in beam potential as the charge builds!
So provided your applications are in what I would call the "super light microscope" area and you want basic EDS, the table top is a great asset. If you are taking your science further you need an instrument with a wider range of accelerating voltages and the option to use secondary electron signals. In either case I would purchase a sputter coater and a carbon head to stabilise difficult specimens; backscatter is not the answer to everything.
That said I do think the "table tops" are great but in the right environment.
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Sent: 17 March 2011 19:27 To: protrain-at-emcourses.com
Hello Jim, I don't have experience with table top SEMs, but your job sounds like what I did at Goodyear, or at least the equipment sounds the same. I agree you don't want a research grade SEM, but I think you will find you need more than a table top.
I found I used both secondary electron (detailed pictures) and backscatter electron (helps me find the areas with different average atomic number for EDS) as well as EDS. I found some plastics charged so bad I needed to gold or carbon coat, and I found the Pd/Pt interfered the least with the elements I was interested in. You should consider a sputter.
An environmental SEM, with provisions for reduced vacuum (and reduced charging) in the sample chamber, proved so useful for looking at difficult to coat samples like carbon black pellets, glassy spheres on conductive tape and so many other specimens, but you can only use backscatter because problems with light caused by an interaction of low pressure gas and electrons.
I can't tell you how many times I've used different acceleration KVs I want 30 KV for S and Mo detection. Surface details on paint, plastic or the insect built in to your product might call for 4kv. I like 25kv for steel but 15kv for thinish films.
Of course not all your specimens will be orientated in the chamber for best imaging or EDS, so the more rotation and tilt you have the better. Don't forget the Z axis. Not all samples will be the same size and I'll forsaken EDS data to increase the Z axis to get lower magnification images. I still wish I could get 10x magnification on some samples. The ability to have different apertures to change the depth of focus has proven invaluable. It's not always about high magnification, sometime you want the peaks and valleys of an irregular surface in focus.
Your experience will be different, but anticipate that your uses in 5 years will not be the same as now. I would suggest a medium range SEM with mag up to 50k and a large chamber will serve you better in the long rum.
And don't forget about the room the scope is going in: low magnetic fields, low vibrations and constant current and voltage are a must to get the best out of your SEM.
Keep us informed.
Frank Karl Lincoln Electric
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 13, 16 -- From frank_karl-at-lincolnelectric.com Thu Mar 17 14:26:04 2011 13, 16 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2HJQ4lG002445 13, 16 -- for {microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 14:26:04 -0500 13, 16 -- Subject: SEM: to table top or not, that is the question 13, 16 -- Sensitivity: 13, 16 -- To: Microscopy-at-microscopy.com, james.m.hoffmann-at-usa.dupont.com 13, 16 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 13, 16 -- Message-ID: {OF6419792A.E4D39524-ON85257856.006A738B-85257856.006AC06C-at-lincolnelectric.c om} 13, 16 -- From: Frank_Karl-at-lincolnelectric.com 13, 16 -- Date: Thu, 17 Mar 2011 15:26:00 -0400 13, 16 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 13, 16 -- 03/17/2011 03:25:59 PM 13, 16 -- MIME-Version: 1.0 13, 16 -- Content-Type: text/plain; 13, 16 -- charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 33, 27 -- From protrain-at-emcourses.com Fri Mar 18 05:18:41 2011 33, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 33, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2IAIelB005805 33, 27 -- for {microscopy-at-microscopy.com} ; Fri, 18 Mar 2011 05:18:40 -0500 33, 27 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:49432 helo=ProtrainPC) 33, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 33, 27 -- (envelope-from {protrain-at-emcourses.com} ) 33, 27 -- id 1Q0WlR-000K7K-8C by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 18 Mar 2011 12:18:38 +0200 33, 27 -- Reply-To: {protrain-at-emcourses.com} 33, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 33, 27 -- To: {Frank_Karl-at-lincolnelectric.com} 33, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 33, 27 -- References: {201103171927.p2HJR2OR003698-at-ns.microscopy.com} 33, 27 -- In-Reply-To: {201103171927.p2HJR2OR003698-at-ns.microscopy.com} 33, 27 -- Subject: RE: [Microscopy] SEM: to table top or not, that is the question 33, 27 -- Date: Fri, 18 Mar 2011 10:18:32 -0000 33, 27 -- Organization: Protrain 33, 27 -- Message-ID: {004501cbe555$d77aa0f0$866fe2d0$-at-com} 33, 27 -- MIME-Version: 1.0 33, 27 -- Content-Type: text/plain; 33, 27 -- charset="us-ascii" 33, 27 -- Content-Transfer-Encoding: 7bit 33, 27 -- X-Mailer: Microsoft Office Outlook 12.0 33, 27 -- Thread-Index: Acvk2VcRK21BOtmHRBmHWlcwNfv9hwAeGN+w 33, 27 -- Content-Language: en-gb 33, 27 -- X-Scan-Signature: 22a7acf3b69f9491b127844b95d5d4b8{152}} 33, 27 -- X-Trace: smtp01.dial-up.net 1Q0WlR-000K7K-8C f9b907ba698b7ddb5d0f6186fbfaac2a ==============================End of - Headers==============================
I forgot to include one test the service engineer and that was to disconnect the HV cable from the HV tank. The leakage current was the same. He did some further test to narrow it down to the cascade device but of course it there could be a cascade of issues that only come to light as each part is repaired.
Thank you for your response about the filament "light". I thought it might be something like that because nothing changes it except for a physical barrier. It indicates that there is no high voltage reaching the gun.
Thanks again, Louie
----- Original Message ----- X-from: "Steve Chapman" { protrain -at- emcourses .com} To: lkerr -at- mbl . edu Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} Sent: Friday, March 18, 2011 5:47:52 AM
Dear All:
I would like to hear comments (on microtome itself and service) from people= who own RMC microtomes. Please email me at hyi-at-emory.edu. Thank you all = very much in advance.
Hong
This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.
If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
==============================Original Headers============================== 6, 36 -- From hyi-at-emory.edu Fri Mar 18 08:05:00 2011 6, 36 -- Received: from mr4.cc.emory.edu (mr4.cc.emory.edu [170.140.52.93]) 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2ID50ua008831 6, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Mar 2011 08:05:00 -0500 6, 36 -- Received: from EXCHEDGE4.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 6, 36 -- by mr4.cc.emory.edu (8.13.1/8.13.1) with ESMTP id p2ID4wkg008740 6, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 18 Mar 2011 09:04:58 -0400 6, 36 -- Received: from EXCHHUB4.Enterprise.emory.net (170.140.30.56) by 6, 36 -- EXCHEDGE4.enterprise.emory.net (170.140.52.36) with Microsoft SMTP Server 6, 36 -- (TLS) id 8.3.137.0; Fri, 18 Mar 2011 09:04:52 -0400 6, 36 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 6, 36 -- EXCHHUB4.Enterprise.emory.net ([170.140.30.56]) with mapi; Fri, 18 Mar 2011 6, 36 -- 09:04:58 -0400 6, 36 -- From: "Yi, Hong" {hyi-at-emory.edu} 6, 36 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 36 -- Date: Fri, 18 Mar 2011 09:04:57 -0400 6, 36 -- Subject: RMC Ultramicrotome 6, 36 -- Thread-Topic: RMC Ultramicrotome 6, 36 -- Thread-Index: AQHL5W0V8Cfd3hiB+UuRu1yApUsYfw== 6, 36 -- Message-ID: {3940C1839888A54DAE85AE20F294FB690130F2AB27F7-at-EXCHANGE12.Enterprise.emory.net} 6, 36 -- Accept-Language: en-US 6, 36 -- Content-Language: en-US 6, 36 -- X-MS-Has-Attach: 6, 36 -- X-MS-TNEF-Correlator: 6, 36 -- acceptlanguage: en-US 6, 36 -- Content-Type: text/plain; charset="us-ascii" 6, 36 -- MIME-Version: 1.0 6, 36 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 6, 36 -- X-Emory-MailScanner-ID: p2ID4wkg008740 6, 36 -- X-Emory-MailScanner: Found to be clean 6, 36 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, score=0, 6, 36 -- required 8, autolearn=disabled) 6, 36 -- X-Emory-MailScanner-From: hyi-at-emory.edu 6, 36 -- X-Spam-Status: No 6, 36 -- Content-Transfer-Encoding: 8bit 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2ID50ua008831 ==============================End of - Headers==============================
} Date: Sat, 19 Mar 2011 01:34:03 -0700 } From: Benjamin Brezler {benjamin.brezler-at-gmail.com} } } Below is the result of your form, submitted on Saturday, March 19, } 2011 at 01:33:49 AM. } } realname - Benjamin Brezler } Email - benjamin.brezler-at-gmail.com } ORGANIZATION - San Francisco State University (SFSU) } EDUCATION - Graduate College } LOCATION - San Francisco, CA, USA } SUBJECT_OF_QUESTION - Staining Microbial Samples for SEM } QUESTION - Dear MSA, } I am a Master's student at SFSU, and my project involves working } with flagellated Gram-negative bacteria. I am trying to compare the } quality and number of flagella in wild-type and mutant strains of } these bacteria. The project has been quite challenging, and I am } hoping to get some advice that will help me stain and image flagella } and pili. I have reviewed several books (by Hayat, Goldstein, and } Bazzola) and several research articles. However, I can't seem to } find a protocol to work with. } } Any advice, suggestions, references, or contacts would be greatly appreciated. } } Thank you, } Benjamin Brezler } } PS: I have been fixing with glutaraldehyde in sodium cacodylate, } dehydrating with an ethanol series, and then trying various } different stains (phosphotungstic acid, uranyl acetate, and osmium } tetroxide), but I'm not sure if I'm using them properly (i.e. at the } right concentrations, pH, staining for long enough, etc.). } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 27 -- From oshel1pe-at-cmich.edu Mon Mar 21 07:08:18 2011 1, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LC8IkK022435 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 07:08:18 -0500 1, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p2LC8GDY017530 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 08:08:17 -0400 1, 27 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 27 -- Mon, 21 Mar 2011 08:08:05 -0400 1, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Mon, 21 Mar 1, 27 -- 2011 08:08:05 -0400 1, 27 -- MIME-Version: 1.0 1, 27 -- Message-ID: {a06240801c9acefd3ee7d-at-[141.209.160.249]} 1, 27 -- Date: Mon, 21 Mar 2011 08:08:03 -0400 1, 27 -- To: {Microscopy-at-microscopy.com} 1, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 27 -- Subject: Bacterial flagella & pili study 1, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 27 -- X-Originating-IP: [141.209.160.249] 1, 27 -- X-OriginalArrivalTime: 21 Mar 2011 12:08:05.0569 (UTC) FILETIME=[A277D310:01CBE7C0] 1, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 27 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 27 -- X-CanItPRO-Stream: default 1, 27 -- X-Canit-Stats-ID: 02ElM8h6n - 4f45bc242112 - 20110321 1, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
The best way to image such fragile structures as flagella and pili is to use negative staining methods. A couple months ago, I obtained some excellent images of flagella in Paenebacillus (grown on agar medium) with a very simple procedure, as follows:
1. Using Formvar and carbon-coated 200 mesh grids (held with a fine tweezers), touch the edge of the colony very lightly with the grid.
2. Float the grid on a droplet of 1% aqueous uranyl acetate for 30 sec. (Note: Keep the UA out of direct light (especially sunlight) as much as possible.)
3. Remove grid and touch edge to a filter paper to wick away stain.
4. Place on a filter paper and allow to dry.
5. Examine in TEM.
If you have a liquid culture, mix one drop of culture with UA and float grid on the mixture for 1 min. Then, follow steps 3-5.
UA worked best for me (versus phosphotungstate); however, it does have some shortcomings. You must avoid phosphate (buffers, etc.) since this will precipitate the UA. Sometimes, as was the case with my own study, you can still use the UA prep, even with the precipitate. However, it is an awful mess and you will spend a lot of time looking for the rare, clean areas on the grid. If this is too messy for you, then you should try 2% phosphotungstate (adjusted to pH 6.5 with 1 N KOH).
I can send you an image, if you like, to show you what to expect.
JB
On Mon, Mar 21, 2011 at 7:09 AM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Date: Sat, 19 Mar 2011 01:34:03 -0700 } } From: Benjamin Brezler {benjamin.brezler-at-gmail.com} } } } } Below is the result of your form, submitted on Saturday, March 19, } } 2011 at 01:33:49 AM. } } } } realname - Benjamin Brezler } } Email - benjamin.brezler-at-gmail.com } } ORGANIZATION - San Francisco State University (SFSU) } } EDUCATION - Graduate College } } LOCATION - San Francisco, CA, USA } } SUBJECT_OF_QUESTION - Staining Microbial Samples for SEM } } QUESTION - Dear MSA, } } I am a Master's student at SFSU, and my project involves working } } with flagellated Gram-negative bacteria. I am trying to compare the } } quality and number of flagella in wild-type and mutant strains of } } these bacteria. The project has been quite challenging, and I am } } hoping to get some advice that will help me stain and image flagella } } and pili. I have reviewed several books (by Hayat, Goldstein, and } } Bazzola) and several research articles. However, I can't  seem to } } find a protocol to work with. } } } } Any advice, suggestions, references, or contacts would be greatly appreciated. } } } } Thank you, } } Benjamin Brezler } } } } PS: I have been fixing with glutaraldehyde in sodium cacodylate, } } dehydrating with an ethanol series, and then trying various } } different stains (phosphotungstic acid, uranyl acetate, and osmium } } tetroxide), but I'm not sure if I'm using them properly (i.e. at the } } right concentrations, pH, staining for long enough, etc.). } } } -- } *************************************************************************************** } Forwarded from "Ask a Microscopist"
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 18, 21 -- From bozzola-at-siu.edu Mon Mar 21 08:51:15 2011 18, 21 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LDpFFb009677 18, 21 -- for {microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 08:51:15 -0500 18, 21 -- Received: by iyf13 with SMTP id 13so8104290iyf.0 18, 21 -- for {microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 06:51:15 -0700 (PDT) 18, 21 -- MIME-Version: 1.0 18, 21 -- Received: by 10.42.96.135 with SMTP id j7mr1100343icn.245.1300715475400; Mon, 18, 21 -- 21 Mar 2011 06:51:15 -0700 (PDT) 18, 21 -- Received: by 10.42.1.146 with HTTP; Mon, 21 Mar 2011 06:51:15 -0700 (PDT) 18, 21 -- In-Reply-To: {201103211209.p2LC9Si7023635-at-ns.microscopy.com} 18, 21 -- References: {201103211209.p2LC9Si7023635-at-ns.microscopy.com} 18, 21 -- Date: Mon, 21 Mar 2011 08:51:15 -0500 18, 21 -- Message-ID: {AANLkTikgRH=RNarrBTBmhUYvv-_wq+EHZjFztRR-Aeku-at-mail.gmail.com} 18, 21 -- Subject: Re: [Microscopy] Bacterial flagella & pili study 18, 21 -- From: John Bozzola {bozzola-at-siu.edu} 18, 21 -- To: oshel1pe-at-cmich.edu, MSAListserver {microscopy-at-microscopy.com} , 18, 21 -- benjamin.brezler-at-gmail.com 18, 21 -- Content-Type: text/plain; charset=UTF-8 18, 21 -- Content-Transfer-Encoding: 8bit 18, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2LDpFFb009677 ==============================End of - Headers==============================
you mention the use of buffered glutaraldehyde and dehydration for observing pili and flagella but the usual method is to simply negative stain unfixed cells. Trying to carry out negative stains on fixed bacteria is difficult and dehydration would not help.
Flagella and pili can be fragile and are best examined from a fresh culture with a simple direct negative stain. Sometimes the medium in the culture is so dense that it can make a good stain difficult. A gentle spin and wash of bacteria helps but can also damage sensitive flagella and pili.
I've found a variety of negative stains useful for pili and flagella. Ammonium molybdate can be very useful for bacteria but potassium phosphotungstate or uranyl acetate will work too. I usually keep the stains as a stock solution of 2% and try dilutions of between 0.5% and 2% (ie 1/4 to neat). The more dilute stains if carefully dried seem to be better for fine pili.
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
----- Original Message ----- X-from: oshel1pe-at-cmich.edu
To reiterate what others have been saying here, pili are very easily disrupted so the shorter the processing the better. I spent ages working on samples for a student who was trying to remove pili to block biofilm formation before I figured out that they'd stripped control samples of pili by simply aspirating the cells with a pipette. 2% uranium acetate for 30 seconds has been about the most reliable method for me, I've got some great images just by dipping a grid into pond water, blotting and spotting on UA. Cheers Ian
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: 21 March 2011 12:18 To: Portman, Ian
} Date: Sat, 19 Mar 2011 01:34:03 -0700 } From: Benjamin Brezler {benjamin.brezler-at-gmail.com} } } Below is the result of your form, submitted on Saturday, March 19, } 2011 at 01:33:49 AM. } } realname - Benjamin Brezler } Email - benjamin.brezler-at-gmail.com } ORGANIZATION - San Francisco State University (SFSU) } EDUCATION - Graduate College } LOCATION - San Francisco, CA, USA } SUBJECT_OF_QUESTION - Staining Microbial Samples for SEM } QUESTION - Dear MSA, } I am a Master's student at SFSU, and my project involves working } with flagellated Gram-negative bacteria. I am trying to compare the } quality and number of flagella in wild-type and mutant strains of } these bacteria. The project has been quite challenging, and I am } hoping to get some advice that will help me stain and image flagella } and pili. I have reviewed several books (by Hayat, Goldstein, and } Bazzola) and several research articles. However, I can't seem to } find a protocol to work with. } } Any advice, suggestions, references, or contacts would be greatly appreciated. } } Thank you, } Benjamin Brezler } } PS: I have been fixing with glutaraldehyde in sodium cacodylate, } dehydrating with an ethanol series, and then trying various } different stains (phosphotungstic acid, uranyl acetate, and osmium } tetroxide), but I'm not sure if I'm using them properly (i.e. at the } right concentrations, pH, staining for long enough, etc.). } -- ************************************************************************ ***************
==============================Original Headers============================== 9, 37 -- From I.J.Portman-at-warwick.ac.uk Mon Mar 21 10:38:33 2011 9, 37 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.csv.warwick.ac.uk [137.205.128.7]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LFcWEg010855 9, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 10:38:33 -0500 9, 37 -- Received: from localhost (localhost [127.0.0.1]) 9, 37 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p2LFc0GW014146; 9, 37 -- Mon, 21 Mar 2011 15:38:27 GMT 9, 37 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 9, 37 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 9, 37 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 9, 37 -- with LMTP id hjWthHX6Wojj; Mon, 21 Mar 2011 15:38:18 +0000 (GMT) 9, 37 -- Received: from beech.ads.warwick.ac.uk (beech [137.205.97.163]) 9, 37 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p2LFawWf013744; 9, 37 -- Mon, 21 Mar 2011 15:36:58 GMT 9, 37 -- X-Envelope-From: I.J.Portman-at-warwick.ac.uk 9, 37 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.181]) by beech.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 9, 37 -- Mon, 21 Mar 2011 15:36:57 +0000 9, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 37 -- Content-class: urn:content-classes:message 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; 9, 37 -- charset="us-ascii" 9, 37 -- Subject: RE: [Microscopy] Bacterial flagella & pili study 9, 37 -- Date: Mon, 21 Mar 2011 15:36:57 -0000 9, 37 -- Message-ID: {DB126BA68A8A5A439BAF072438A39FEA0294DB68-at-ELDER.ads.warwick.ac.uk} 9, 37 -- In-Reply-To: {201103211217.p2LCHrD9000744-at-ns.microscopy.com} 9, 37 -- X-MS-Has-Attach: 9, 37 -- X-MS-TNEF-Correlator: 9, 37 -- Thread-Topic: [Microscopy] Bacterial flagella & pili study 9, 37 -- Thread-Index: AcvnwqipBrZdFqyfQOKWEGbl+ojiAwAGduBA 9, 37 -- References: {201103211217.p2LCHrD9000744-at-ns.microscopy.com} 9, 37 -- From: "Portman, Ian" {I.J.Portman-at-warwick.ac.uk} 9, 37 -- To: {benjamin.brezler-at-gmail.com} , {Microscopy-at-microscopy.com} 9, 37 -- X-OriginalArrivalTime: 21 Mar 2011 15:36:57.0548 (UTC) FILETIME=[D01C10C0:01CBE7DD] 9, 37 -- X-DCC-Warwick-Metrics: bluebell; whitelist 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2LFcWEg010855 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both degraef-at-cmu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: degraef-at-cmu.edu Name: Marc DeGraef
Organization: Carnegie Mellon University
Title-Subject: [Filtered] Workshop on In-situ and remote electron microscopy
Message: Dear colleague,
every two years or so an international workshop is held on the topics of remote and in-situ microscopy (both SEM and TEM based). The third workshop in this series will be held at Carnegie Mellon University in Pittsburgh, USA, on June 6-8, 2011. The previous two workshops were held at Stanford (Bob Sinclair) and at Goteborg (Eva Olsson); the size of the workshop is anticipated to be around 80 people. Several microscope and related equipment vendors will also be present.
On behalf of the organizers, I would like to invite you to attend this workshop. All information can be found on the web site http://mpg.web.cmu.edu/CMU-IWREMISS-2011.html. Registration (on a first-come, first -serve basis) is free for academic members and students, $400 for industrial attendees. There will be a continuous poster session, so if you have anything you wish to contribute, let us know and we will accommodate your poster.
For more information, please contact Marc DeGraef at degraef-at-cmu.edu.
What is a reasonable length of time to wait for a beam to stabilize? I take a series of 256 images with a 120kV TEM and have found that I have to wait one to two hours for the beam current to stabilize sufficiently to provide equidense images for montaging or analysis. The beam instability ocurrs even without apertures or specimen inserted and with both tungsten and Lab6 filaments--the Lab6 does seem to take longer to stabilize.
Thanks, Larry -- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S1355 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 4, 34 -- From btv1==06159213984==Larry.Ackerman-at-ucsf.edu Mon Mar 21 15:37:15 2011 4, 34 -- Received: from bcuda2.ucsf.edu (bcuda2.ucsf.edu [64.54.247.214]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LKbElE016905 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 15:37:15 -0500 4, 34 -- X-ASG-Debug-ID: 1300739834-0108e940c711dc20001-1DjkGe 4, 34 -- Received: from EXHT02.net.ucsf.edu (mx.net.ucsf.edu [64.54.247.193]) by bcuda2.ucsf.edu with ESMTP id bL7jQmWLlxHwCtDl (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 13:37:14 -0700 (PDT) 4, 34 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 4, 34 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 4, 34 -- Received: from Ralston-Lab-iMac.local (128.218.239.127) by EXHT02.net.ucsf.edu 4, 34 -- (64.54.247.219) with Microsoft SMTP Server id 8.3.137.0; Mon, 21 Mar 2011 4, 34 -- 13:37:13 -0700 4, 34 -- Message-ID: {4D87B6FB.5030708-at-ucsf.edu} 4, 34 -- Date: Mon, 21 Mar 2011 13:37:15 -0700 4, 34 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 4, 34 -- Reply-To: larry.ackerman-at-ucsf.edu 4, 34 -- Organization: Electron Microscopy Lab, UCSF 4, 34 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812) 4, 34 -- MIME-Version: 1.0 4, 34 -- To: {Microscopy-at-microscopy.com} 4, 34 -- Subject: Beam Stability 4, 34 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 34 -- X-ASG-Orig-Subj: Beam Stability 4, 34 -- Content-Transfer-Encoding: 7bit 4, 34 -- X-Barracuda-Connect: mx.net.ucsf.edu[64.54.247.193] 4, 34 -- X-Barracuda-Start-Time: 1300739834 4, 34 -- X-Barracuda-Encrypted: AES128-SHA 4, 34 -- X-Barracuda-URL: http://bcuda2.ucsf.edu:80/cgi-mod/mark.cgi 4, 34 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 4, 34 -- X-Barracuda-Spam-Score: 0.00 4, 34 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=9.0 KILL_LEVEL=5.0 tests= 4, 34 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.58573 4, 34 -- Rule breakdown below 4, 34 -- pts rule name description 4, 34 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
Larry, There are 2 areas that need to stabilize, both thermally. One is the high voltage power supply. The components change value slightly with temperature so the output (actual kV and filament current) may drift slightly until everything equilibrates.
The other area is the gun. The cathode to wehnelt distance changes as the gun changes temperature (so does the wehnelt to anode distance). That spacing is far more critical for LaB6, hence the longer time to stabilize.
My SEM customers who do ebeam lithography generally don't even reduce their Lab6 cathodes to the standby temperature. They leave the gun fully at temperature and saturated. The cost is that the cathodes get replaced every 6 months. Since they often operate at 40kV (high for an SEM), I always had them reduce the kV to 20 when they weren't using the system. Aside from protecting the high voltage power supply and some of the gun components, this also keeps the lenses warm. Yup, that's a third area that needs to stabilize thermally, but it doesn't seem to be as critical as the first two areas.
If your TEM is stabilizing in 1-2 hours from a cold start, I'm stunned! The thermal masses involved in both the power supply and the gun are probably far greater than most SEMs. If it takes that long from a semi-warmed up standby situation, then there might possibly be some kind of issue, but maybe not. 1-2 hours for high stability doesn't strike me as at all unreasonable.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu] Sent: Monday, March 21, 2011 4:40 PM To: kenconverse-at-qualityimages.biz
What is a reasonable length of time to wait for a beam to stabilize? I take a series of 256 images with a 120kV TEM and have found that I have to wait one to two hours for the beam current to stabilize sufficiently to provide equidense images for montaging or analysis. The beam instability ocurrs even without apertures or specimen inserted and with both tungsten and Lab6 filaments--the Lab6 does seem to take longer to stabilize.
Thanks, Larry -- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S1355 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 4, 34 -- From btv1==06159213984==Larry.Ackerman-at-ucsf.edu Mon Mar 21 15:37:15 2011 4, 34 -- Received: from bcuda2.ucsf.edu (bcuda2.ucsf.edu [64.54.247.214]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LKbElE016905 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 15:37:15 -0500 4, 34 -- X-ASG-Debug-ID: 1300739834-0108e940c711dc20001-1DjkGe 4, 34 -- Received: from EXHT02.net.ucsf.edu (mx.net.ucsf.edu [64.54.247.193]) by bcuda2.ucsf.edu with ESMTP id bL7jQmWLlxHwCtDl (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 13:37:14 -0700 (PDT) 4, 34 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 4, 34 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 4, 34 -- Received: from Ralston-Lab-iMac.local (128.218.239.127) by EXHT02.net.ucsf.edu 4, 34 -- (64.54.247.219) with Microsoft SMTP Server id 8.3.137.0; Mon, 21 Mar 2011 4, 34 -- 13:37:13 -0700 4, 34 -- Message-ID: {4D87B6FB.5030708-at-ucsf.edu} 4, 34 -- Date: Mon, 21 Mar 2011 13:37:15 -0700 4, 34 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 4, 34 -- Reply-To: larry.ackerman-at-ucsf.edu 4, 34 -- Organization: Electron Microscopy Lab, UCSF 4, 34 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812) 4, 34 -- MIME-Version: 1.0 4, 34 -- To: {Microscopy-at-microscopy.com} 4, 34 -- Subject: Beam Stability 4, 34 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 34 -- X-ASG-Orig-Subj: Beam Stability 4, 34 -- Content-Transfer-Encoding: 7bit 4, 34 -- X-Barracuda-Connect: mx.net.ucsf.edu[64.54.247.193] 4, 34 -- X-Barracuda-Start-Time: 1300739834 4, 34 -- X-Barracuda-Encrypted: AES128-SHA 4, 34 -- X-Barracuda-URL: http://bcuda2.ucsf.edu:80/cgi-mod/mark.cgi 4, 34 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 4, 34 -- X-Barracuda-Spam-Score: 0.00 4, 34 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=9.0 KILL_LEVEL=5.0 tests= 4, 34 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.58573 4, 34 -- Rule breakdown below 4, 34 -- pts rule name description 4, 34 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From kenconverse-at-qualityimages.biz Mon Mar 21 17:01:53 2011 19, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LM1qmb002111 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 17:01:53 -0500 19, 28 -- X-Authority-Analysis: v=1.1 cv=Nm3SJc7L3wlcojC9snsyzORkYWw1JOu3BeZkTeIwPUk= c=1 sm=0 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=N0QMrkfzAAAA:8 a=9cWz7c8GAAAA:8 a=2vT069InAAAA:8 a=RTcWGElIZct9aRaPvfkA:9 a=N3ou6iWBVju4LeYai2YA:7 a=pDRRr10xGFn5XNK5x-ZYILAh_LEA:4 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=fM1NKZWFy18A:10 a=Q28L5ce9E3UA:10 a=nbSwaPR1tzIA:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=nv6_9D8q1JFd7MpE:21 a=fWnzi4HCYYq3n816:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 19, 28 -- X-Cloudmark-Score: 0 19, 28 -- X-Originating-IP: 72.224.149.57 19, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2297] helo=Ken) 19, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 19, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 19, 28 -- id 9A/CB-11439-0DAC78D4; Mon, 21 Mar 2011 22:01:52 +0000 19, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 19, 28 -- To: {larry.ackerman-at-ucsf.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 19, 28 -- Subject: RE: [Microscopy] Beam Stability 19, 28 -- Date: Mon, 21 Mar 2011 18:01:46 -0400 19, 28 -- Message-ID: {B777222D23244F9783484F13BB675649-at-Ken} 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="us-ascii" 19, 28 -- X-Priority: 3 (Normal) 19, 28 -- X-MSMail-Priority: Normal 19, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 19, 28 -- In-Reply-To: {201103212039.p2LKdhM6020404-at-ns.microscopy.com} 19, 28 -- Thread-Index: AcvoCBuQDNsTpzujSbSQoDfJPn73RQACMCCg 19, 28 -- Importance: Normal 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2LM1qmb002111 ==============================End of - Headers==============================
The time to stabilize that I mentioned starts when the filament is heated after a sample change. The high voltage is never off--for months and the lenses at 40,000X when not doing microscopy which is typically 1000--10,000X
Thanks, Larry
Ken Converse wrote: } Larry, } There are 2 areas that need to stabilize, both thermally. One is the high } voltage power supply. The components change value slightly with temperature } so the output (actual kV and filament current) may drift slightly until } everything equilibrates. } } The other area is the gun. The cathode to wehnelt distance changes as the } gun changes temperature (so does the wehnelt to anode distance). That } spacing is far more critical for LaB6, hence the longer time to stabilize. } } My SEM customers who do ebeam lithography generally don't even reduce their } Lab6 cathodes to the standby temperature. They leave the gun fully at } temperature and saturated. The cost is that the cathodes get replaced every } 6 months. Since they often operate at 40kV (high for an SEM), I always had } them reduce the kV to 20 when they weren't using the system. Aside from } protecting the high voltage power supply and some of the gun components, } this also keeps the lenses warm. Yup, that's a third area that needs to } stabilize thermally, but it doesn't seem to be as critical as the first two } areas. } } If your TEM is stabilizing in 1-2 hours from a cold start, I'm stunned! The } thermal masses involved in both the power supply and the gun are probably } far greater than most SEMs. If it takes that long from a semi-warmed up } standby situation, then there might possibly be some kind of issue, but } maybe not. 1-2 hours for high stability doesn't strike me as at all } unreasonable. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } Fax 207-647-2688 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } From: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu] } Sent: Monday, March 21, 2011 4:40 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Beam Stability } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } What is a reasonable length of time to wait for a beam to stabilize? I } take a series of 256 images with a 120kV TEM and have found that I have } to wait one to two hours for the beam current to stabilize sufficiently } to provide equidense images for montaging or analysis. The beam } instability ocurrs even without apertures or specimen inserted and with } both tungsten and Lab6 filaments--the Lab6 does seem to take longer to } stabilize. } } Thanks, } Larry
-- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S1355 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 6, 37 -- From btv1==06159213984==Larry.Ackerman-at-ucsf.edu Mon Mar 21 17:14:38 2011 6, 37 -- Received: from bcuda2.ucsf.edu (bcuda2.ucsf.edu [64.54.247.214]) 6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LMEcmj018259 6, 37 -- for {microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 17:14:38 -0500 6, 37 -- X-ASG-Debug-ID: 1300745583-0108e940c71280f0025-4CH8be 6, 37 -- Received: from EXHT01.net.ucsf.edu (mx.net.ucsf.edu [64.54.247.193]) by bcuda2.ucsf.edu with ESMTP id RDlu9sNM9PHFtP1a (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 15:14:29 -0700 (PDT) 6, 37 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 6, 37 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 6, 37 -- Received: from Ralston-Lab-iMac.local (128.218.239.127) by EXHT01.net.ucsf.edu 6, 37 -- (64.54.247.218) with Microsoft SMTP Server id 8.3.137.0; Mon, 21 Mar 2011 6, 37 -- 15:14:03 -0700 6, 37 -- Message-ID: {4D87CDAD.3060705-at-ucsf.edu} 6, 37 -- Date: Mon, 21 Mar 2011 15:14:05 -0700 6, 37 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 6, 37 -- Reply-To: larry.ackerman-at-ucsf.edu 6, 37 -- Organization: Electron Microscopy Lab, UCSF 6, 37 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812) 6, 37 -- MIME-Version: 1.0 6, 37 -- CC: MSA Listserver {microscopy-at-microscopy.com} 6, 37 -- Subject: Re: [Microscopy] Beam Stability 6, 37 -- References: {B777222D23244F9783484F13BB675649-at-Ken} 6, 37 -- X-ASG-Orig-Subj: Re: [Microscopy] Beam Stability 6, 37 -- In-Reply-To: {B777222D23244F9783484F13BB675649-at-Ken} 6, 37 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 37 -- Content-Transfer-Encoding: 7bit 6, 37 -- X-Barracuda-Connect: mx.net.ucsf.edu[64.54.247.193] 6, 37 -- X-Barracuda-Start-Time: 1300745669 6, 37 -- X-Barracuda-Encrypted: AES128-SHA 6, 37 -- X-Barracuda-URL: http://bcuda2.ucsf.edu:80/cgi-mod/mark.cgi 6, 37 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 6, 37 -- X-Barracuda-Spam-Score: 1.21 6, 37 -- X-Barracuda-Spam-Status: No, SCORE=1.21 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=9.0 KILL_LEVEL=5.0 tests=MISSING_HEADERS 6, 37 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.58579 6, 37 -- Rule breakdown below 6, 37 -- pts rule name description 6, 37 -- ---- ---------------------- -------------------------------------------------- 6, 37 -- 1.21 MISSING_HEADERS Missing To: header ==============================End of - Headers==============================
} What is a reasonable length of time to wait for a beam to stabilize? I } take a series of 256 images with a 120kV TEM and have found that I have } to wait one to two hours for the beam current to stabilize sufficiently } to provide equidense images for montaging or analysis. ....
to this point: in our faculty-EM-facility, we are using a 120 keV TEM, vintage 1990 - daily, with (regularly) 10+X different users. Standard voltage is 120 keV, always, since more than 18 years. LaB6, always; lifetime; about 3 or up to 3.5 years . Vacuum excellent and stable: Rot, ODP plus IGP, plus always LN2; cryo cycle (IGP off) every evening for 4 hrs; computer-controlled HT and filament start every morning, with wobbling the HT at 120 keV. -- LaB6 is cold for specimen change, always; re-heated for 19 x 5 sec, each time. After this, we can start recording "equidense" images almost immediately (after 3 to 5 min "searching"). -- Yes, some Master or even phD students don't see or experience a change of a filament. (I have an old one, on the bench, for demo). We take digital micrographs on a 12bit 1k camera since 12 years, with lots of stitching, daily, and never experience problems with instability or uneven or varying density (at least not observed / observable!). We do take montages with usually 4 x 4, up to 8 x 8 (or more) frames. - If there are problems, then they are related to "too old" dark and flat fields , which are used for correcting the digital images. Dark/Flat fields are needed about once a week, but sometimes only in 2 or 3 weeks. (no cryo work at very low dose!) -- ((only problems when working with new or old filament)) kind regards - Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 6, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Mon Mar 21 17:28:14 2011 6, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LMSD9D002029 6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 17:28:13 -0500 6, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 6, 25 -- by localhost (Postfix) with SMTP id C2DB5CA6 6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 23:28:12 +0100 (CET) 6, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 6, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id AAB286A9 6, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 23:28:12 +0100 (CET) 6, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 6, 25 -- with Novell_GroupWise; Mon, 21 Mar 2011 23:28:12 +0100 6, 25 -- Message-Id: {4D87DF080200005400021C4D-at-gwsmtp1.uni-regensburg.de} 6, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 6, 25 -- Date: Mon, 21 Mar 2011 23:28:08 +0100 6, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 6, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 6, 25 -- Subject: Beam Stability 6, 25 -- References: {201103212202.p2LM2E8N002436-at-ns.microscopy.com} 6, 25 -- In-Reply-To: {201103212202.p2LM2E8N002436-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=US-ASCII 6, 25 -- Content-Disposition: inline 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2LMSD9D002029 ==============================End of - Headers==============================
After many years of taking commissioning test pictures to prove the performance of TEM and SEM it is clear there are a number of features that need to be taken into account.
In my early days as a service technician (probably 1965 to 1970) I was amazed how it always took me about three hours to "get in the groove" when I was taking pictures for final commissioning. Then I noticed if I simply switched the high voltage on, with the specimen in the instrument, and to return about two to three hours later, I had a test picture within half an hour. Eureka, when I then realised about high voltage stability - heat gained must equal heat lost.
Having a very nice meal with a client one evening he suggested that we go back to the lab and I take a test picture on the TEM that they have just moved from one lab to another. With the same simplistic reasoning I thought "Oh no three hours!". But I had forgot the instrument had a gas filled tank and was thrilled to within forty five minutes have the test resolution actually visible on the screen; no picture required! Silly me, I always through they gave us a gas filled tank so it was less of a mess going in to change components, not that it cut down mass!
Working with automated analytical SEM we found that the backscattered electron results and x-ray emission did not become stable for some hours after specimen exchange. Investigating the problem colleagues found that when the vacuum in the gun became absolutely stable so did the results! Outgassing the samples prior to placing them in the microscope halved the time for stability to be reached.
Of course a cathode assembly will need to become thermally stable, but it is the other areas that take the longest time.
So putting all the knowledge together here are the results-
Transmission Instruments
1. High voltage generator - the critical period is the time taken for heat gained to equal heat lost once the high voltage has been switched on
Which relates to the type of high voltage tank (generator box if you like) a) Oil filled tanks take up to three hours to stabilise depending on how large the tank is. b) Gas filled tanks generally take about an hour or so to reach stability.
2. The vacuum system in the gun also has to be absolutely stable, the speed of pumping matching the leakage. This will relate to when the gun chamber was last opened.
Scanning Instruments
1. The high voltage tank is very much smaller and in general stability is reached within a hour.
2. If the electron gun is brought to atmosphere each time a specimen is exchanged, depending how gassy the routine specimens are, it may take over two hours for the gun vacuum to stabilise.
Both of these sets of comment assume that there are no electronic problems with the instruments high voltage circuitry.
Hope this helps.
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: larry.ackerman-at-ucsf.edu [mailto:larry.ackerman-at-ucsf.edu] Sent: 21 March 2011 20:38 To: protrain-at-emcourses.com
What is a reasonable length of time to wait for a beam to stabilize? I take a series of 256 images with a 120kV TEM and have found that I have to wait one to two hours for the beam current to stabilize sufficiently to provide equidense images for montaging or analysis. The beam instability ocurrs even without apertures or specimen inserted and with both tungsten and Lab6 filaments--the Lab6 does seem to take longer to stabilize.
Thanks, Larry -- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes & Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S1355 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 4, 34 -- From btv1==06159213984==Larry.Ackerman-at-ucsf.edu Mon Mar 21 15:37:15 2011 4, 34 -- Received: from bcuda2.ucsf.edu (bcuda2.ucsf.edu [64.54.247.214]) 4, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LKbElE016905 4, 34 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 15:37:15 -0500 4, 34 -- X-ASG-Debug-ID: 1300739834-0108e940c711dc20001-1DjkGe 4, 34 -- Received: from EXHT02.net.ucsf.edu (mx.net.ucsf.edu [64.54.247.193]) by bcuda2.ucsf.edu with ESMTP id bL7jQmWLlxHwCtDl (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 13:37:14 -0700 (PDT) 4, 34 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 4, 34 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 4, 34 -- Received: from Ralston-Lab-iMac.local (128.218.239.127) by EXHT02.net.ucsf.edu 4, 34 -- (64.54.247.219) with Microsoft SMTP Server id 8.3.137.0; Mon, 21 Mar 2011 4, 34 -- 13:37:13 -0700 4, 34 -- Message-ID: {4D87B6FB.5030708-at-ucsf.edu} 4, 34 -- Date: Mon, 21 Mar 2011 13:37:15 -0700 4, 34 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 4, 34 -- Reply-To: larry.ackerman-at-ucsf.edu 4, 34 -- Organization: Electron Microscopy Lab, UCSF 4, 34 -- User-Agent: Thunderbird 2.0.0.23 (Macintosh/20090812) 4, 34 -- MIME-Version: 1.0 4, 34 -- To: {Microscopy-at-microscopy.com} 4, 34 -- Subject: Beam Stability 4, 34 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 4, 34 -- X-ASG-Orig-Subj: Beam Stability 4, 34 -- Content-Transfer-Encoding: 7bit 4, 34 -- X-Barracuda-Connect: mx.net.ucsf.edu[64.54.247.193] 4, 34 -- X-Barracuda-Start-Time: 1300739834 4, 34 -- X-Barracuda-Encrypted: AES128-SHA 4, 34 -- X-Barracuda-URL: http://bcuda2.ucsf.edu:80/cgi-mod/mark.cgi 4, 34 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 4, 34 -- X-Barracuda-Spam-Score: 0.00 4, 34 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=9.0 KILL_LEVEL=5.0 tests= 4, 34 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.58573 4, 34 -- Rule breakdown below 4, 34 -- pts rule name description 4, 34 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
==============================Original Headers============================== 30, 27 -- From protrain-at-emcourses.com Tue Mar 22 04:57:06 2011 30, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 30, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2M9v5m9030751 30, 27 -- for {microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 04:57:06 -0500 30, 27 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:49437 helo=ProtrainPC) 30, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 30, 27 -- (envelope-from {protrain-at-emcourses.com} ) 30, 27 -- id 1Q1yKj-000FTl-W5 by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Tue, 22 Mar 2011 11:57:02 +0200 30, 27 -- Reply-To: {protrain-at-emcourses.com} 30, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 30, 27 -- To: {larry.ackerman-at-ucsf.edu} 30, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 30, 27 -- References: {201103212038.p2LKcLrM018130-at-ns.microscopy.com} 30, 27 -- In-Reply-To: {201103212038.p2LKcLrM018130-at-ns.microscopy.com} 30, 27 -- Subject: RE: [Microscopy] Beam Stability 30, 27 -- Date: Tue, 22 Mar 2011 09:56:54 -0000 30, 27 -- Organization: Protrain 30, 27 -- Message-ID: {001901cbe877$7c1d5340$7457f9c0$-at-com} 30, 27 -- MIME-Version: 1.0 30, 27 -- Content-Type: text/plain; 30, 27 -- charset="us-ascii" 30, 27 -- Content-Transfer-Encoding: 7bit 30, 27 -- X-Mailer: Microsoft Office Outlook 12.0 30, 27 -- Thread-Index: AcvoB/fMDcBxRP8gTu2DLbVt3qcWXAAaqeEQ 30, 27 -- Content-Language: en-gb 30, 27 -- X-Scan-Signature: fb0fa83a71d025ff5324124052ee9744{160}} 30, 27 -- X-Trace: smtp01.dial-up.net 1Q1yKj-000FTl-W5 7c96ca97f2efb323a54fd8726ff1eb06 ==============================End of - Headers==============================
} Hi } } After many years of taking commissioning test pictures to prove the } performance of TEM and SEM it is clear there are a number of features that } need to be taken into account. } } In my early days as a service technician (probably 1965 to 1970) I was } amazed how it always took me about three hours to "get in the groove" when I } was taking pictures for final commissioning.  Then I noticed if I simply } switched the high voltage on, with the specimen in the instrument, and to } return about two to three hours later, I had a test picture within half an } hour.  Eureka, when I then realised about high voltage stability - heat } gained must equal heat lost. } } Having a very nice meal with a client one evening he suggested that we go } back to the lab and I take a test picture on the TEM that they have just } moved from one lab to another. With the same simplistic reasoning I thought } "Oh no three hours!".  But I had forgot the instrument had a gas filled tank } and was thrilled to within forty five minutes have the test resolution } actually visible on the screen; no picture required!  Silly me, I always } through they gave us a gas filled tank so it was less of a mess going in to } change components, not that it cut down mass! } } Working with automated analytical SEM we found that the backscattered } electron results and x-ray emission did not become stable for some hours } after specimen exchange.  Investigating the problem colleagues found that } when the vacuum in the gun became absolutely stable so did the results! } Outgassing the samples prior to placing them in the microscope halved the } time for stability to be reached. } } Of course a cathode assembly will need to become thermally stable, but it is } the other areas that take the longest time. } } So putting all the knowledge together here are the results- } } Transmission Instruments } } 1.    High voltage generator - the critical period is the time taken for } heat gained to equal heat lost  once the high voltage has been switched on } }     Which relates to the type of high voltage tank (generator box if you } like) }     a)    Oil filled tanks take up to three hours to stabilise } depending on how large the tank is. }     b)    Gas filled tanks generally take about an hour or so to reach } stability. } } 2.    The vacuum system in the gun also has to be absolutely stable, the } speed of pumping matching the leakage.  This will relate to when the gun } chamber was last opened. } } Scanning Instruments } } 1.    The high voltage tank is very much smaller and in general stability } is reached within a hour. } } 2.    If the electron gun is brought to atmosphere each time a specimen is } exchanged, depending how gassy the routine specimens are, it may take over } two hours for the gun vacuum to stabilise. } } Both of these sets of comment assume that there are no electronic problems } with the instruments high voltage circuitry. } } Hope this helps. } } Steve } } Steve Chapman FRMS } Senior Consultant Protrain } For consultancy and training in electron microscopy world wide } Tel +44 (0)1280 816512  Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 } www.emcourses.com } -- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, IL 62901 Phone: 618-453-3730
==============================Original Headers============================== 5, 20 -- From bozzola-at-siu.edu Tue Mar 22 08:13:08 2011 5, 20 -- Received: from mail-gy0-f169.google.com (mail-gy0-f169.google.com [209.85.160.169]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2MDD8N5017926 5, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 08:13:08 -0500 5, 20 -- Received: by gyg10 with SMTP id 10so2876789gyg.0 5, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 06:13:08 -0700 (PDT) 5, 20 -- MIME-Version: 1.0 5, 20 -- Received: by 10.43.57.195 with SMTP id wh3mr137339icb.513.1300799588065; Tue, 5, 20 -- 22 Mar 2011 06:13:08 -0700 (PDT) 5, 20 -- Received: by 10.42.1.146 with HTTP; Tue, 22 Mar 2011 06:13:08 -0700 (PDT) 5, 20 -- In-Reply-To: {201103220958.p2M9wDJx031940-at-ns.microscopy.com} 5, 20 -- References: {201103220958.p2M9wDJx031940-at-ns.microscopy.com} 5, 20 -- Date: Tue, 22 Mar 2011 08:13:08 -0500 5, 20 -- Message-ID: {AANLkTim+uWGS9HvMeMqPB9e_Brj9r6NtKsPrWn-E=NS0-at-mail.gmail.com} 5, 20 -- Subject: Re: [Microscopy] RE: Beam Stability 5, 20 -- From: John Bozzola {bozzola-at-siu.edu} 5, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 20 -- Content-Type: text/plain; charset=UTF-8 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2MDD8N5017926 ==============================End of - Headers==============================
There is an old SEM (Hitachi s-650) in the lab that I work in and it has worked fine for a number of years. Upon turning it on a couple of months ago the displays weren't working correctly.
In normal operation the scan line starts at the bottom of the screen, but now there is a line across the middle of each the screen where the vertical scan starts. When you push the waveform button the line scan is present at the middle of the screen rather than the bottom. It does not react to a increase in current. From the gauge you can tell that the current is increasing across the filament and upon inspection the filament is not ruined.
Under normal scanning it scans down from the line in screen 1 to the bottom and then from the top of screen 2 down to the line.
I believe that it is an electronics issue based on the behavior and as the machine is not exactly new, one of the boards could have gone bad. If anyone has any suggestion I would greatly appreciate it.
Thanks for your time,
Kyle
Kyle Colavito Mechanical Engineering University of Arizona Work (520) 626-5225 Lab (520) 621-6092 Cell (520)250-8409 kcolavit-at-u.arizona.edu kyle.colavito-at-gmail.com
==============================Original Headers============================== 12, 33 -- From kyle.colavito-at-gmail.com Tue Mar 22 12:58:11 2011 12, 33 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2MHwAH0008262 12, 33 -- for {microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 12:58:10 -0500 12, 33 -- Received: by wyf19 with SMTP id 19so7931130wyf.0 12, 33 -- for {microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 10:58:10 -0700 (PDT) 12, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 33 -- d=gmail.com; s=gamma; 12, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 12, 33 -- :content-type:content-transfer-encoding; 12, 33 -- bh=bw65JUgLcX65bZ60rtNPF0i47VEGP8uNx33Q9sCfOV8=; 12, 33 -- b=NecwHonA6E9VcSAPArpaNO6NXXCnewwWJr5mkLBFhs8CHHWgF3U7l7jKl1BKDnrwLz 12, 33 -- 1stOOtPMfUHz38TN+wrkHM062Kdej9ndRcvNjzNcqomG8WD+V/cef4GjikkJWmfQM/AW 12, 33 -- Gu3pbD92R2mixUtt8nEXHGwQEqlKrEJqbTFgs= 12, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 12, 33 -- d=gmail.com; s=gamma; 12, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 12, 33 -- :content-transfer-encoding; 12, 33 -- b=ccyaQqFq8+zrZQjWQ2kSUZYQ3CoxsPJkocJxHeHkLkeQSon8yTdT7IE1NgfXRAGHA1 12, 33 -- FGENrqBhlkQafKlM4oaGeotuXjLoKAQeBKrodi+aTJ9PaSJV2E9yAznIWwRAGcFzfjdq 12, 33 -- BZsa3ZZ2r1Q6JFhyXZeJhwGp3I3GKaO7J6S6M= 12, 33 -- MIME-Version: 1.0 12, 33 -- Received: by 10.216.142.34 with SMTP id h34mr6624272wej.28.1300816689128; Tue, 12, 33 -- 22 Mar 2011 10:58:09 -0700 (PDT) 12, 33 -- Received: by 10.216.122.129 with HTTP; Tue, 22 Mar 2011 10:58:09 -0700 (PDT) 12, 33 -- Date: Tue, 22 Mar 2011 10:58:09 -0700 12, 33 -- Message-ID: {AANLkTi=pFO_+xXYT6Uvmr3FoRH5gR16YQ=Ja+mFH2DRD-at-mail.gmail.com} 12, 33 -- Subject: SEM - Need help troubleshooting a Hitachi S-650 12, 33 -- From: kyle colavito {kyle.colavito-at-gmail.com} 12, 33 -- To: microscopy {microscopy-at-microscopy.com} 12, 33 -- Content-Type: text/plain; charset=ISO-8859-1 12, 33 -- Content-Transfer-Encoding: 8bit 12, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2MHwAH0008262 ==============================End of - Headers==============================
We have a microscope/RGB digital camera/PC winXP setup which uses a Scion CG7 Framegrabber (~6 years old).. the PC just went up in smoke and of course the drivers for the framegrabber are nowhere to be found.. and scioncorp.com (and their phone#s) are no longer alive..
It seems a pity to junk a framegrabber and camera for lack of a driver.. does anybody have one of these that might be able to give me a copy of the installation software?
thanks!
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation.
==============================Original Headers============================== 8, 22 -- From cpawlowicz-at-ubmtechinsights.com Tue Mar 22 14:50:01 2011 8, 22 -- Received: from pegasus.semiconductor.com (semi-fw.ubmtechinsights.com [207.61.67.10]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2MJo0pr027086 8, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 14:50:01 -0500 8, 22 -- Received: from pegasus.semiconductor.com ([192.168.2.10]) by pegasus 8, 22 -- ([192.168.2.10]) with mapi; Tue, 22 Mar 2011 15:49:59 -0400 8, 22 -- From: Chris Pawlowicz {cpawlowicz-at-ubmtechinsights.com} 8, 22 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 8, 22 -- Date: Tue, 22 Mar 2011 15:49:57 -0400 8, 22 -- Subject: Scion framegrabber drivers? 8, 22 -- Thread-Topic: Scion framegrabber drivers? 8, 22 -- Thread-Index: AcvoylJojndFm05xR7Owm408NynhCQ== 8, 22 -- Message-ID: {0079587FD46DFC42AC07C9D337C7C895395FD0EE94-at-pegasus} 8, 22 -- Accept-Language: en-US 8, 22 -- Content-Language: en-US 8, 22 -- X-MS-Has-Attach: 8, 22 -- X-MS-TNEF-Correlator: 8, 22 -- acceptlanguage: en-US 8, 22 -- Content-Type: text/plain; charset="us-ascii" 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Transfer-Encoding: 8bit 8, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2MJo0pr027086 ==============================End of - Headers==============================
It looks like something went wrong with Y deflection electronics.
Considering age of the instrument, you may need to get help from one of those old-timers who still remembers how to do component-level repair.
Good luck!
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 3/22/2011 1:59 PM, kyle.colavito-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, } } There is an old SEM (Hitachi s-650) in the lab that I work in and it has } worked fine for a number of years. Upon turning it on a couple of months } ago the displays weren't working correctly. } } In normal operation the scan line starts at the bottom of the screen, but } now there is a line across the middle of each the screen where the vertical } scan starts. When you push the waveform button the line scan is present at } the middle of the screen rather than the bottom. It does not react to a } increase in current. From the gauge you can tell that the current is } increasing across the filament and upon inspection the filament is not } ruined. } } Under normal scanning it scans down from the line in screen 1 to the bottom } and then from the top of screen 2 down to the line. } } Screen 1 } ______________ } | | } | | } | | } |------------------------| } | | } | | } |______________| } } Screen 2 } ______________ } | | } | | } |------------------------| } | | } | | } | | } |______________| } } } I believe that it is an electronics issue based on the behavior and as the } machine is not exactly new, one of the boards could have gone bad. If } anyone has any suggestion I would greatly appreciate it. } } Thanks for your time, } } Kyle } } Kyle Colavito } Mechanical Engineering } University of Arizona } Work (520) 626-5225 } Lab (520) 621-6092 } Cell (520)250-8409 } kcolavit-at-u.arizona.edu } kyle.colavito-at-gmail.com } } } ==============================Original Headers============================== } 12, 33 -- From kyle.colavito-at-gmail.com Tue Mar 22 12:58:11 2011 } 12, 33 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) } 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2MHwAH0008262 } 12, 33 -- for {microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 12:58:10 -0500 } 12, 33 -- Received: by wyf19 with SMTP id 19so7931130wyf.0 } 12, 33 -- for {microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 10:58:10 -0700 (PDT) } 12, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 33 -- d=gmail.com; s=gamma; } 12, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 12, 33 -- :content-type:content-transfer-encoding; } 12, 33 -- bh=bw65JUgLcX65bZ60rtNPF0i47VEGP8uNx33Q9sCfOV8=; } 12, 33 -- b=NecwHonA6E9VcSAPArpaNO6NXXCnewwWJr5mkLBFhs8CHHWgF3U7l7jKl1BKDnrwLz } 12, 33 -- 1stOOtPMfUHz38TN+wrkHM062Kdej9ndRcvNjzNcqomG8WD+V/cef4GjikkJWmfQM/AW } 12, 33 -- Gu3pbD92R2mixUtt8nEXHGwQEqlKrEJqbTFgs= } 12, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 12, 33 -- d=gmail.com; s=gamma; } 12, 33 -- h=mime-version:date:message-id:subject:from:to:content-type } 12, 33 -- :content-transfer-encoding; } 12, 33 -- b=ccyaQqFq8+zrZQjWQ2kSUZYQ3CoxsPJkocJxHeHkLkeQSon8yTdT7IE1NgfXRAGHA1 } 12, 33 -- FGENrqBhlkQafKlM4oaGeotuXjLoKAQeBKrodi+aTJ9PaSJV2E9yAznIWwRAGcFzfjdq } 12, 33 -- BZsa3ZZ2r1Q6JFhyXZeJhwGp3I3GKaO7J6S6M= } 12, 33 -- MIME-Version: 1.0 } 12, 33 -- Received: by 10.216.142.34 with SMTP id h34mr6624272wej.28.1300816689128; Tue, } 12, 33 -- 22 Mar 2011 10:58:09 -0700 (PDT) } 12, 33 -- Received: by 10.216.122.129 with HTTP; Tue, 22 Mar 2011 10:58:09 -0700 (PDT) } 12, 33 -- Date: Tue, 22 Mar 2011 10:58:09 -0700 } 12, 33 -- Message-ID: {AANLkTi=pFO_+xXYT6Uvmr3FoRH5gR16YQ=Ja+mFH2DRD-at-mail.gmail.com} } 12, 33 -- Subject: SEM - Need help troubleshooting a Hitachi S-650 } 12, 33 -- From: kyle colavito {kyle.colavito-at-gmail.com} } 12, 33 -- To: microscopy {microscopy-at-microscopy.com} } 12, 33 -- Content-Type: text/plain; charset=ISO-8859-1 } 12, 33 -- Content-Transfer-Encoding: 8bit } 12, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2MHwAH0008262 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 34 -- From vray-at-partbeamsystech.com Tue Mar 22 14:59:27 2011 6, 34 -- Received: from nm7-vm0.bullet.mail.ac4.yahoo.com (nm7-vm0.bullet.mail.ac4.yahoo.com [98.139.52.228]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2MJxRus010879 6, 34 -- for {microscopy-at-microscopy.com} ; Tue, 22 Mar 2011 14:59:27 -0500 6, 34 -- Received: from [98.139.52.194] by nm7.bullet.mail.ac4.yahoo.com with NNFMP; 22 Mar 2011 19:59:26 -0000 6, 34 -- Received: from [98.139.52.167] by tm7.bullet.mail.ac4.yahoo.com with NNFMP; 22 Mar 2011 19:59:26 -0000 6, 34 -- Received: from [127.0.0.1] by omp1050.mail.ac4.yahoo.com with NNFMP; 22 Mar 2011 19:59:26 -0000 6, 34 -- X-Yahoo-Newman-Id: 771765.4921.bm-at-omp1050.mail.ac4.yahoo.com 6, 34 -- Received: (qmail 91884 invoked from network); 22 Mar 2011 19:59:26 -0000 6, 34 -- Received: from [192.168.2.5] (vray-at-75.68.231.25 with plain) 6, 34 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 22 Mar 2011 12:59:25 -0700 PDT 6, 34 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 34 -- X-YMail-OSG: reZQ7zEVM1lYi.SbsmYBWAuX0a0Aeh__qKV8_C5pdKunAQD 6, 34 -- fdsRf6NMSDbhnoasye8EcT_OwSHtoaruXVwpvbs6kT34zB51yeKOTi8qF5TH 6, 34 -- 190FjLdmDB1lm5k_qZli6CgmKLwTi7bQ3slTVmCG69fnT4BL3ATJQoGc.kAy 6, 34 -- 9Gbz3lpujSe90kA8xmyyN_XnLnC0ORctvNXj63DgbeLsK5JrnYPAAp3QBXKc 6, 34 -- u0JDzXvU308MQ216bsNSiNm6Z.rakaHBYo80vzldoyPghGf9ZqW6oIcvXbqC 6, 34 -- tg_WaiTko59nyubFNj_t27bwSyF14gnUn8_AEgfofjA9LPqYclrir8z78mUt 6, 34 -- HuTJth7eCiLefanN0FvxuPawNmRjSBc3.0EY8bfj_Z6I6 6, 34 -- X-Yahoo-Newman-Property: ymail-3 6, 34 -- Message-ID: {4D88FFA0.5050209-at-partbeamsystech.com} 6, 34 -- Date: Tue, 22 Mar 2011 15:59:28 -0400 6, 34 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 6, 34 -- Reply-To: vray-at-partbeamsystech.com 6, 34 -- Organization: PBS&T 6, 34 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.15) Gecko/20110303 Lightning/1.0b2 Thunderbird/3.1.9 6, 34 -- MIME-Version: 1.0 6, 34 -- To: kyle.colavito-at-gmail.com 6, 34 -- CC: microscopy-at-microscopy.com 6, 34 -- Subject: Re: [Microscopy] SEM - Need help troubleshooting a Hitachi S-650 6, 34 -- References: {201103221759.p2MHxjiu009680-at-ns.microscopy.com} 6, 34 -- In-Reply-To: {201103221759.p2MHxjiu009680-at-ns.microscopy.com} 6, 34 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 34 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone have a service manual for the S360, and would they be willing to relinquish it long enough for me to get a copy somehow? We already have the operator manual and circuit diagrams, but would actually like to know precisely how twiddling a knob changes what we see on the screen.
thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: nyu
Title-Subject: [Filtered] looking for a second-hand 2 photon upright microscope
Message: We have a colleague who is looking for a second-hand 2 photon upright microscope. Any information including reputable websites that sell used microscopes greatly appreciated; please contact offline. Thank you!
________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
We have closed our darkrooms and are giving these away:
Durst Laborator 138S enlarger with all the lenses. It is attached to a drop down table.
Ted Pella Nitrogen Burst Film Developing system.
Safe Lights, we have quite a few. The big ones, with the hinged lids.
3 Stainless steel containers to store EM film under vacuum. These are currently set up in series (all 3 attached to each other).
You must pay shipping and you must take it ASAP.
First come, first served, etc..
Contact me for more information.
Paula
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.3452
==============================Original Headers============================== 12, 33 -- From patpxs-at-gmail.com Wed Mar 23 06:07:56 2011 12, 33 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 12, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2NB7ul8013451 12, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 06:07:56 -0500 12, 33 -- Received: by iwl42 with SMTP id 42so11022351iwl.0 12, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 04:07:56 -0700 (PDT) 12, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 33 -- d=gmail.com; s=gamma; 12, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 12, 33 -- :content-type:content-transfer-encoding; 12, 33 -- bh=Y1ayv2tV8TZCTZXoD0wsFmaLetZo49OvSsEEfO58IDU=; 12, 33 -- b=C4kVR2nl7aq63odAxp6a0LGqRQZtqI9lwPKOtoC4YjW95t1C6eglWr6q/Fd1Qi6S33 12, 33 -- fQ4CMBwnwI7Ld2E4ry8HSRju1lYFzTQrYO2RtQF0aorMWp3f/0f256uC4USWvMLZTIOh 12, 33 -- KGiBcoi9oZwR3DB+gXIJBalLpjq9WONox7bXc= 12, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 12, 33 -- d=gmail.com; s=gamma; 12, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 12, 33 -- :content-transfer-encoding; 12, 33 -- b=IJtZnEOFPgJ1zlatzdfTBlftOXyk+oyJYOownZ/DVbpN3GCv3+lZZJbefRBeheo3Jy 12, 33 -- 8/sIBmy/VQZpUXb/DxQQfTRVZr+y5FHHYFNyCPFLSu50qMJWImIGKKn9BAYHExZ4Ot25 12, 33 -- u/NmV08F85AJzHhIsjzyfHZUYoFS2dr9H5DzE= 12, 33 -- MIME-Version: 1.0 12, 33 -- Received: by 10.42.136.1 with SMTP id r1mr8340835ict.15.1300878475999; Wed, 23 12, 33 -- Mar 2011 04:07:55 -0700 (PDT) 12, 33 -- Received: by 10.42.148.134 with HTTP; Wed, 23 Mar 2011 04:07:55 -0700 (PDT) 12, 33 -- Date: Wed, 23 Mar 2011 07:07:55 -0400 12, 33 -- Message-ID: {AANLkTi=5JKriAGNo8NqSsobCK8jwDDoESib71VCcg5DQ-at-mail.gmail.com} 12, 33 -- Subject: Darkroom Items Free, You Pay Shipping 12, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 12, 33 -- To: Microscopy-at-microscopy.com 12, 33 -- Content-Type: text/plain; charset=ISO-8859-1 12, 33 -- Content-Transfer-Encoding: 8bit 12, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2NB7ul8013451 ==============================End of - Headers==============================
Although negative staining is definitively a valuable solution, another elegant technique which gives very nice contrast for such thin structures is shadow casting. Manipulation, as already stated, must be kept minimal and this is also a reason why shadow casting works well. Just google for "flagella/flagellum shadow casting". Have fun as well as success.
Stephane
----- Original Message ---- X-from: "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu} To: nizets2-at-yahoo.com Sent: Mon, March 21, 2011 1:14:18 PM
} Date: Sat, 19 Mar 2011 01:34:03 -0700 } From: Benjamin Brezler {benjamin.brezler-at-gmail.com} } } Below is the result of your form, submitted on Saturday, March 19, } 2011 at 01:33:49 AM. } } realname - Benjamin Brezler } Email - benjamin.brezler-at-gmail.com } ORGANIZATION - San Francisco State University (SFSU) } EDUCATION - Graduate College } LOCATION - San Francisco, CA, USA } SUBJECT_OF_QUESTION - Staining Microbial Samples for SEM } QUESTION - Dear MSA, } I am a Master's student at SFSU, and my project involves working } with flagellated Gram-negative bacteria. I am trying to compare the } quality and number of flagella in wild-type and mutant strains of } these bacteria. The project has been quite challenging, and I am } hoping to get some advice that will help me stain and image flagella } and pili. I have reviewed several books (by Hayat, Goldstein, and } Bazzola) and several research articles. However, I can't seem to } find a protocol to work with. } } Any advice, suggestions, references, or contacts would be greatly appreciated. } } Thank you, } Benjamin Brezler } } PS: I have been fixing with glutaraldehyde in sodium cacodylate, } dehydrating with an ethanol series, and then trying various } different stains (phosphotungstic acid, uranyl acetate, and osmium } tetroxide), but I'm not sure if I'm using them properly (i.e. at the } right concentrations, pH, staining for long enough, etc.). } -- ***************************************************************************************
Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 27 -- From oshel1pe-at-cmich.edu Mon Mar 21 07:08:18 2011 1, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2LC8IkK022435 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 07:08:18 -0500 1, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p2LC8GDY017530 1, 27 -- for {Microscopy-at-microscopy.com} ; Mon, 21 Mar 2011 08:08:17 -0400 1, 27 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 27 -- Mon, 21 Mar 2011 08:08:05 -0400 1, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Mon, 21 Mar 1, 27 -- 2011 08:08:05 -0400 1, 27 -- MIME-Version: 1.0 1, 27 -- Message-ID: {a06240801c9acefd3ee7d-at-[141.209.160.249]} 1, 27 -- Date: Mon, 21 Mar 2011 08:08:03 -0400 1, 27 -- To: {Microscopy-at-microscopy.com} 1, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 27 -- Subject: Bacterial flagella & pili study 1, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 27 -- X-Originating-IP: [141.209.160.249] 1, 27 -- X-OriginalArrivalTime: 21 Mar 2011 12:08:05.0569 (UTC) FILETIME=[A277D310:01CBE7C0] 1, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 27 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 27 -- X-CanItPRO-Stream: default 1, 27 -- X-Canit-Stats-ID: 02ElM8h6n - 4f45bc242112 - 20110321 1, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 37 -- From nizets2-at-yahoo.com Wed Mar 23 09:34:28 2011 16, 37 -- Received: from nm24.bullet.mail.sp2.yahoo.com (nm24.bullet.mail.sp2.yahoo.com [98.139.91.94]) 16, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2NEYRII001506 16, 37 -- for {microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 09:34:27 -0500 16, 37 -- Received: from [98.139.91.62] by nm24.bullet.mail.sp2.yahoo.com with NNFMP; 23 Mar 2011 14:34:26 -0000 16, 37 -- Received: from [98.139.91.58] by tm2.bullet.mail.sp2.yahoo.com with NNFMP; 23 Mar 2011 14:34:26 -0000 16, 37 -- Received: from [127.0.0.1] by omp1058.mail.sp2.yahoo.com with NNFMP; 23 Mar 2011 14:34:26 -0000 16, 37 -- X-Yahoo-Newman-Property: ymail-3 16, 37 -- X-Yahoo-Newman-Id: 822815.46078.bm-at-omp1058.mail.sp2.yahoo.com 16, 37 -- Received: (qmail 2460 invoked by uid 60001); 23 Mar 2011 14:34:26 -0000 16, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1300890866; bh=/Nx3sM16d/obIFnzn+GPn3BkDg3+XYkr4a7vejbddLo=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=WVKYW+tNMZYJQhNLM0pkov4kP6PORKFHuXbs172gSu7Yo+F0cIuJjEWrHzofcvpKh69WTeHxSySCMITJsDGBMnXlPg/iptali4HiGpY7kA/TAvrxyqrp8Jn1fGjmSuNmeu8HO73L4On1oKpHalTDHd9uLv0WtixofLAYcm/biB0= 16, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 37 -- s=s1024; d=yahoo.com; 16, 37 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 16, 37 -- b=eNQKEz6XB/m7xo2k6sEUgyIHkwCJokKg8MCIQdGKuP1sHVFxouaryLdUn96te9qwqsHJHTs/BvYliWW8hhX/tNpakJcWNK2CtsoFX4e974b9HWnN5GObjlCx9B4dcZ92HdHw4Am+Oo/KQ9t1sVVEYiqpyN4bmx25qThf6PoM5xk=; 16, 37 -- Message-ID: {247644.99121.qm-at-web110804.mail.gq1.yahoo.com} 16, 37 -- X-YMail-OSG: QowsLpAVM1m0b1fhH5_GG_0dFaH.kT0eBAWRrHx5fplSIyo 16, 37 -- O0zwhMJ8s_VaYwtRXJtwJ8rFIRAyeISjSil4s4BejX3nC8A6o9xhAXN.PASY 16, 37 -- YHVL10RJ4zlanoJiskJrmMfqOdDMmdFHcNmYSGcBPpo8EviX23S8UNsTbHxK 16, 37 -- nzn5Bm2HVhwE81QCZ40OYrCtEaJm60C8baa0GchNyw9bVnqt71A1U_A0KfOL 16, 37 -- 8Gen89u0szEjS1bLG3quzgh25J54krYqSBEVm_Nprf31MeKgaaCwhfI8.kVI 16, 37 -- U3O4MhaZ68NHyHJof6BoGwSotwscf72me7sFrAVjeuWBJllB1qo4PmucQ9PA 16, 37 -- MrmMQZWGo5jRKS1zzln2Tyyq2ROEBC29PCEi8zBEBm0gGTD5vfxk9xwqn.Om 16, 37 -- uXIuS0fTn5hKEio6s 16, 37 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Wed, 23 Mar 2011 07:34:26 PDT 16, 37 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 16, 37 -- References: {201103211214.p2LCEIKF028273-at-ns.microscopy.com} 16, 37 -- Date: Wed, 23 Mar 2011 07:34:26 -0700 (PDT) 16, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 16, 37 -- Subject: Re: [Microscopy] Bacterial flagella & pili study 16, 37 -- To: benjamin.brezler-at-gmail.com 16, 37 -- Cc: microscopy-at-microscopy.com 16, 37 -- In-Reply-To: {201103211214.p2LCEIKF028273-at-ns.microscopy.com} 16, 37 -- MIME-Version: 1.0 16, 37 -- Content-Type: text/plain; charset=iso-8859-1 16, 37 -- Content-Transfer-Encoding: 8bit 16, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2NEYRII001506 ==============================End of - Headers==============================
I've made some progress into figuring out the problem with my machine, but am having a hard time without any schematics. If anyone has a copy that would be greatly appreciated.
Thanks, Kyle
Kyle Colavito Mechanical Engineering University of Arizona Work (520) 626-5225 Lab (520) 621-6092 Cell (520)250-8409 kcolavit-at-u.arizona.edu kyle.colavito-at-gmail.com
==============================Original Headers============================== 3, 30 -- From kyle.colavito-at-gmail.com Wed Mar 23 14:41:00 2011 3, 30 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2NJexHO008675 3, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 14:40:59 -0500 3, 30 -- Received: by vxk12 with SMTP id 12so7972046vxk.0 3, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 12:40:59 -0700 (PDT) 3, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 30 -- d=gmail.com; s=gamma; 3, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 3, 30 -- :content-type; 3, 30 -- bh=fjPNDz0lbQ3EGF1Qk1N86gdFve+djKI01FEGUc5omfk=; 3, 30 -- b=VOAc5Nodzd26I3exwmAolhbzo7vDZbk7ikdCy0alQaHktSlMmYrpPo6Jxc3thQL8T4 3, 30 -- FvI5+dIH3Elk+1SO8hxtfLIsOfr815yik4LKsPHZ3mX1z6eZdUEqY5dgkQV9a3bMZSQN 3, 30 -- FVBsgH4olLmKOfM/KiulsnUG9CuYMtJOLhnxE= 3, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 30 -- d=gmail.com; s=gamma; 3, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 30 -- b=qsL1kAUnnVN/yER3eUEEvzw6RcRT1ey8OeAYi3HCrx9JN9L7tHXQtmd47qbNcymfXb 3, 30 -- gYXEIN/xThcO89TtRQ5z66lq7JJ5vHv/BqX77sWqt476Hn/RcVmmpoxCj/afRZyo7hSx 3, 30 -- uVDD0OkUX0hgkksMf8MhybZmNI9Gtf4AMxXpg= 3, 30 -- MIME-Version: 1.0 3, 30 -- Received: by 10.52.18.11 with SMTP id s11mr407534vdd.213.1300909259425; Wed, 3, 30 -- 23 Mar 2011 12:40:59 -0700 (PDT) 3, 30 -- Received: by 10.52.168.98 with HTTP; Wed, 23 Mar 2011 12:40:59 -0700 (PDT) 3, 30 -- Date: Wed, 23 Mar 2011 12:40:59 -0700 3, 30 -- Message-ID: {AANLkTi=4u8vrin+CbJOS1HYk28=SPW8qrD9C-TLqpDVM-at-mail.gmail.com} 3, 30 -- Subject: SEM - Schematics needed for Hitachi s-650 or similiar model 3, 30 -- From: kyle colavito {kyle.colavito-at-gmail.com} 3, 30 -- To: microscopy {Microscopy-at-microscopy.com} 3, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Got a new sputter coater. Need help determining the sample to target distance. Instructions don't have a recommendation, just a note that says the specimen height is adjustable. Any advice?
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 7, 34 -- From jkrupp-at-deltacollege.edu Wed Mar 23 16:59:16 2011 7, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2NLxF9B027844 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 16:59:15 -0500 7, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 7, 34 -- by localhost (Email Security Appliance) with SMTP id 1515C968321_D8A6D31B 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 21:59:13 +0000 (GMT) 7, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 7, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id F3D13967FFE_D8A6D30F 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 21:59:12 +0000 (GMT) 7, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 7, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 7, 34 -- ESMTP id LIJ6EO00.0Q2 for {Microscopy-at-microscopy.com} ; Wed, 23 7, 34 -- Mar 2011 14:59:12 -0700 7, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id B94E97A383E7 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 14:59:12 -0700 (PDT) 7, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 7, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 34 -- with ESMTP id dCSZgpGMcrWG for {Microscopy-at-microscopy.com} ; 7, 34 -- Wed, 23 Mar 2011 14:59:09 -0700 (PDT) 7, 34 -- Received: from [172.20.12.24] (unknown [172.20.12.24]) 7, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 6FEC07A38379 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 14:59:09 -0700 (PDT) 7, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 7, 34 -- Content-Type: text/plain; charset=us-ascii 7, 34 -- Subject: Target distance? 7, 34 -- Date: Wed, 23 Mar 2011 14:59:09 -0700 7, 34 -- Message-Id: {78C6C78A-2A94-4693-96FC-041D25DD9DF2-at-deltacollege.edu} 7, 34 -- To: Microscopy-at-microscopy.com 7, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 7, 34 -- X-Mailer: Apple Mail (2.1084) 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2NLxF9B027844 ==============================End of - Headers==============================
A generic guide to setting the specimen position in a sputter coater is 5cms target to specimen surface. This means you are in the plasma but not too close to the target.
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: 23 March 2011 22:00 To: protrain-at-emcourses.com
Got a new sputter coater. Need help determining the sample to target distance. Instructions don't have a recommendation, just a note that says the specimen height is adjustable. Any advice?
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 7, 34 -- From jkrupp-at-deltacollege.edu Wed Mar 23 16:59:16 2011 7, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2NLxF9B027844 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 16:59:15 -0500 7, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 7, 34 -- by localhost (Email Security Appliance) with SMTP id 1515C968321_D8A6D31B 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 21:59:13 +0000 (GMT) 7, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 7, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id F3D13967FFE_D8A6D30F 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 21:59:12 +0000 (GMT) 7, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 7, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 7, 34 -- ESMTP id LIJ6EO00.0Q2 for {Microscopy-at-microscopy.com} ; Wed, 23 7, 34 -- Mar 2011 14:59:12 -0700 7, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id B94E97A383E7 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 14:59:12 -0700 (PDT) 7, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 7, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 34 -- with ESMTP id dCSZgpGMcrWG for {Microscopy-at-microscopy.com} ; 7, 34 -- Wed, 23 Mar 2011 14:59:09 -0700 (PDT) 7, 34 -- Received: from [172.20.12.24] (unknown [172.20.12.24]) 7, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 6FEC07A38379 7, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 14:59:09 -0700 (PDT) 7, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 7, 34 -- Content-Type: text/plain; charset=us-ascii 7, 34 -- Subject: Target distance? 7, 34 -- Date: Wed, 23 Mar 2011 14:59:09 -0700 7, 34 -- Message-Id: {78C6C78A-2A94-4693-96FC-041D25DD9DF2-at-deltacollege.edu} 7, 34 -- To: Microscopy-at-microscopy.com 7, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 7, 34 -- X-Mailer: Apple Mail (2.1084) 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2NLxF9B027844 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 27 -- From protrain-at-emcourses.com Wed Mar 23 17:14:47 2011 20, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 20, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2NMEkVi011735 20, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 17:14:47 -0500 20, 27 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:52960 helo=ProtrainPC) 20, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 20, 27 -- (envelope-from {protrain-at-emcourses.com} ) 20, 27 -- id 1Q2WKB-000DSz-8Z by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Thu, 24 Mar 2011 00:14:44 +0200 20, 27 -- Reply-To: {protrain-at-emcourses.com} 20, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 27 -- To: {jkrupp-at-deltacollege.edu} 20, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 20, 27 -- References: {201103232200.p2NM0LLu029103-at-ns.microscopy.com} 20, 27 -- In-Reply-To: {201103232200.p2NM0LLu029103-at-ns.microscopy.com} 20, 27 -- Subject: RE: [Microscopy] Target distance? 20, 27 -- Date: Wed, 23 Mar 2011 22:14:13 -0000 20, 27 -- Organization: Protrain 20, 27 -- Message-ID: {000601cbe9a7$b3983ca0$1ac8b5e0$-at-com} 20, 27 -- MIME-Version: 1.0 20, 27 -- Content-Type: text/plain; 20, 27 -- charset="us-ascii" 20, 27 -- Content-Transfer-Encoding: 7bit 20, 27 -- X-Mailer: Microsoft Office Outlook 12.0 20, 27 -- Thread-Index: AcvppcCUgSvS9wfERnqg2Z4gsE8JnwAAa1LQ 20, 27 -- Content-Language: en-gb 20, 27 -- X-Scan-Signature: 3ad54a49af76538399be1989406aa9e0{104}} 20, 27 -- X-Trace: smtp01.dial-up.net 1Q2WKB-000DSz-8Z 56806dd19c00f6bcd87e787a4a4be2bc ==============================End of - Headers==============================
I'm seeking response from those who's got some experience with the human lung biopsy - any tips, warnings? pitfalls?.. I am quite familiar with TEM embedding in general and also aware about Google and Pubmed... I don't send others there, please don't post just to send me there.
Need to process for standard thin section TEM a lung biopsy that has been stored in the fridge for a year and a half, yes in the original fixative - 1.5%GA+1.5%FA, presumably in PB. The lung, luckily for that person, got replaced by a transplant and is a severe case of certain fibrosis (no asbestos or other "mineral" stuff expected"). The pieces are *huge* - 2 dimensions 10 mm, the 3rd 5 mm tapering off to where the lung ends. So - any advice before I start? Will it be hard to infiltrate?... need more than 1% OsO4?.. things like that...
Thanks! Vlad
________________________________________________ Vlad Speransky, Staff Scientist Biomedical Engineering and Physical Science Shared Resource National Institute of Biomedical Imaging and Bioengineering, NIH 13 South Dr, Rm. 3N17 MSC 5766 Bethesda, MD 20892 301 496-3989 vladislav_speransky-at-nih.gov http://www.nibib.nih.gov/Research/Intramural/SharedResource/Speransky
==============================Original Headers============================== 5, 27 -- From speransv-at-mail.nih.gov Wed Mar 23 19:24:39 2011 5, 27 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2O0Od4C029605 5, 27 -- for {microscopy-at-microscopy.com} ; Wed, 23 Mar 2011 19:24:39 -0500 5, 27 -- X-IronPortListener: Outbound_SMTP 5, 27 -- X-IronPort-Anti-Spam-Filtered: true 5, 27 -- X-IronPort-Anti-Spam-Result: Av0EAAksik2cKEdK/2dsb2JhbACka1p3qVCZZ4MWglMEkFs 5, 27 -- Received: from unknown (HELO NIHHTRC.nih.gov) ([156.40.71.74]) 5, 27 -- by nihxway3out.hub.nih.gov with ESMTP; 23 Mar 2011 20:24:38 -0400 5, 27 -- Received: from NIHMLBX02.nih.gov ([156.40.71.32]) by NIHHTRC.nih.gov 5, 27 -- ([156.40.71.74]) with mapi; Wed, 23 Mar 2011 20:24:38 -0400 5, 27 -- From: "Speransky, Vlad (NIH/NIBIB) [E]" {speransv-at-mail.nih.gov} 5, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 27 -- Date: Wed, 23 Mar 2011 20:24:38 -0400 5, 27 -- Subject: TEM embedding: human lung biopsy?.. 5, 27 -- Thread-Topic: TEM embedding: human lung biopsy?.. 5, 27 -- Thread-Index: AQHL6a86RB+clSyOpUeJ3FtM3SJH7Q== 5, 27 -- Message-ID: {D13763A4740B07428566B25D7E768DA6136665BF07-at-NIHMLBX02.nih.gov} 5, 27 -- Accept-Language: en-US 5, 27 -- Content-Language: en-US 5, 27 -- X-MS-Has-Attach: 5, 27 -- X-MS-TNEF-Correlator: 5, 27 -- acceptlanguage: en-US 5, 27 -- Content-Type: text/plain; charset="us-ascii" 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2O0Od4C029605 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea.greene-at-wdc.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: andrea.greene-at-wdc.com Name: Andrea Greene
Organization: Western Digital Title-Subject: [Filtered] Dualbeam FIB analyst needed
Message: Western Digital Media, San Jose, CA: Six month contract position available immediately for analyst with hands-on expertise using dualbeam FIB to produce ultrathin TEM lamellae on FEI Helios system. Experience with SEM/EDX and manual polish for TEM preparation are a plus.
For your information we teach the following procedure for setting up a sputter coater. Each test, other than the first, uses a piece of normal copy paper cut down to about 2ins X 3ins to simulate the outgassing that would be typical of a specimen
1. Using a piece of paper that covers the stage area, run the coater for several minutes at 20mA. Hold the paper down with a stub placed at the centre. The coat will demonstrate the coverage of the plasma and pick out areas that are well coated or not so well coated. The coating should be pretty perfect with a new coater but patchy with a coater that has a contaminated target. If there are areas with less of a coat you need to map your stage for selecting the best position for superior coating in the future? 2. Place a test paper on the stage held down by a stub. The next step depends upon the voltage of the coater (a) using a low voltage coater (400v to 600v) set the current at 10mA and coat for 30 seconds. (b) with a high voltage coater(} 800v) set the current at 20mA and run for 30 seconds. Note the current and time on the back of the test paper. 3. Repeat the experiment in steps of 30 seconds until the paper starts to exhibit a very slight gold sheen. Note the information on the back of each picture. 4. Select the condition for higher magnification work that shows the first grey colouration. 5. Low magnification operation will probably require the procedure set out in 3.
Sputter coating is often treated as a no brainer, but as those attending our course in Missouri later in the year will see, we dedicate an hour to correctly judge and set up a sputter coater for the first time. It is that important if you wish to obtain information that is not influenced by the coating; just as is the selection of the correct coating material.
For top class SEM results I prefer to use the full facility of the SEM, balancing specimen position, kV and probe current rather than coating. However sometimes you just have to fall back on that route, particularly for novice operators where it is so much easier to slap on a coat to keep life simple.
Enjoy your new coater!
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: Jon Krupp [mailto:jkrupp-at-deltacollege.edu] Sent: 23 March 2011 22:48 To: protrain-at-emcourses.com
Dear all!
My question is not directly related to microscopy but it comes from the observation of a classical methodology used for the preparation of carbon film for EM. I also know that a significant part of the list is made of mineralogists and I think they would be the right persons to answer this question.
Electron microscopists use freshly cleaved mica to prepare carbon films. But why does it need to be freshly cleaved? What property of mica is modified with time? Is it the hydration of the surface, or charging? I would be interested in hearing your comments about the surface modications which occur with time in freshly cleaved or freshly milled rocks. I remember I read a paper which showed that freshly fractured quartz produces more free radicals than aged quartz and I wonder which mechanism can be responsible for that. How can, for example, the surface charge (or zeta potential) be modified with time? Many thanks in advance,
Stephane
==============================Original Headers============================== 7, 34 -- From nizets2-at-yahoo.com Thu Mar 24 08:07:16 2011 7, 34 -- Received: from nm28.bullet.mail.sp2.yahoo.com (nm28.bullet.mail.sp2.yahoo.com [98.139.91.98]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2OD7FYJ006072 7, 34 -- for {microscopy-at-microscopy.com} ; Thu, 24 Mar 2011 08:07:15 -0500 7, 34 -- Received: from [98.139.91.66] by nm28.bullet.mail.sp2.yahoo.com with NNFMP; 24 Mar 2011 13:07:14 -0000 7, 34 -- Received: from [98.139.91.38] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 24 Mar 2011 13:07:14 -0000 7, 34 -- Received: from [127.0.0.1] by omp1038.mail.sp2.yahoo.com with NNFMP; 24 Mar 2011 13:07:14 -0000 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-Yahoo-Newman-Id: 734262.2117.bm-at-omp1038.mail.sp2.yahoo.com 7, 34 -- Received: (qmail 64210 invoked by uid 60001); 24 Mar 2011 13:07:14 -0000 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1300972034; bh=dkj+E2doKP0TASwPkcVbF1DUTdZ+iyB6xG/sq19j6lk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=xzxitJW8uqS9oXxZoHwlno3oQXsP64vqVpiaFNclLPEsOmr4won5QlpkZiQkjak3ZkpCN/vLc5tN3f3phSna6WNHESW41ULehzUsgpOEHEfkdVbL4xZjbAu1pNafkUjzEsZDssBSoyzuO6ER7KCb2dYA6UAOtFJRf6c8BY6EwfY= 7, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 34 -- s=s1024; d=yahoo.com; 7, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 34 -- b=NEs8dujFXGBKJrPI0j/QodlPYpXSfqNxwiAXq7mUSL+g/cwk2twe6OfPkDuwlS/HIcC/aq/7S6wDBpJwj8QVdbrfhl82L0TrX5N1Tx+HwAAMERK7IhMvSp/EelFWjeRVgBkegixmPVzKyKt1bMVOBlY/Qb7mMIhswTaqyq2YfgM=; 7, 34 -- Message-ID: {183789.63573.qm-at-web110815.mail.gq1.yahoo.com} 7, 34 -- X-YMail-OSG: 4PCmHAEVM1nzW3s7VESVHnxlEmoQOUy55wH97Tr.rw9Qz8A 7, 34 -- 5a7Ip7ijgYhWVcqyNArk8rrLuFNfUgK03AlDbq35IzCzKCcCbFP_ZhXuCGR5 7, 34 -- CQaadaoAqMfGgVM9XukH4ssNSiZ1uY4M0mn04d8jOYolhDXFm2OeoLEEG5RS 7, 34 -- pVysP_.EaLfABnMwv6.pzFQPAZ6EZbyYHcEYcBwaGYZF6NxxylAnsDoGSssO 7, 34 -- XVyTMWsO1Umi2.nOst65UFaJY_iouV1W_1acGeJzTiJdOGyRhzKHn6TdkY18 7, 34 -- WmuOE5oYhB1ZgF4_NJ3YpqhmwiAyyz90fh.xQbyjNub6D5nXKRJ5mwWpYTHD 7, 34 -- 9CUAz00qClB5eNl6auLpvk2bc8qPNVYkEYfMwv2GMDFCKcmiG0pA2p4yFDKr 7, 34 -- jUeTUK1NU7ZAaCw-- 7, 34 -- Received: from [80.122.101.100] by web110815.mail.gq1.yahoo.com via HTTP; Thu, 24 Mar 2011 06:07:14 PDT 7, 34 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 7, 34 -- Date: Thu, 24 Mar 2011 06:07:14 -0700 (PDT) 7, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 34 -- Subject: question to geologists/mineralogists 7, 34 -- To: microscopy-at-microscopy.com 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; charset=iso-8859-1 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2OD7FYJ006072 ==============================End of - Headers==============================
We had found EDS counts suddenly dropped almost half after a couple of days no operations of our INCA/Jeol 6400 SEM system. Counts for standard materials of Ni drops from ~10k to ~5k, and noise (zero) peak goes up to about 100k compared to normal of 1-3k, we also noticed a significant “Si peak†at 1.75 KeV in spectrum of pure Ni. Is anybody had this sort of problem before or has idea what it could be? please advice. We are trying to do conditioning detector, i.e. de-ice, to see if any improvement, but still could not explain this sudden counts drop.
Any comments are highly appreciated,
Thanks,
William Zhe Central Analytical Facility Laurentian University
==============================Original Headers============================== 6, 16 -- From wzhe-at-laurentian.ca Thu Mar 24 11:26:45 2011 6, 16 -- Received: from lugwout.laurentian.ca (lugwout.laurentian.ca [142.51.1.65]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2OGQjhw026264 6, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Mar 2011 11:26:45 -0500 6, 16 -- Received: from outdom-MTA by lugwout.laurentian.ca 6, 16 -- with Novell_GroupWise; Thu, 24 Mar 2011 12:25:45 -0400 6, 16 -- Message-Id: {4D8B3872.8E8D.00C5.1-at-laurentian.ca} 6, 16 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 6, 16 -- Date: Thu, 24 Mar 2011 12:26:23 -0400 6, 16 -- From: "William Zhe" {wzhe-at-laurentian.ca} 6, 16 -- To: {Microscopy-at-microscopy.com} 6, 16 -- Subject: EDS counts problem 6, 16 -- Mime-Version: 1.0 6, 16 -- Content-Type: text/plain; charset=UTF-8 6, 16 -- Content-Transfer-Encoding: 8bit 6, 16 -- Content-Disposition: inline ==============================End of - Headers==============================
Fascinating stuff.. including the comments from others *also* building their own SEMs.. who knew?
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation.
==============================Original Headers============================== 8, 22 -- From cpawlowicz-at-ubmtechinsights.com Thu Mar 24 15:17:12 2011 8, 22 -- Received: from pegasus.semiconductor.com (semi-fw.ubmtechinsights.com [207.61.67.10]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2OKHCUu014348 8, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Mar 2011 15:17:12 -0500 8, 22 -- Received: from pegasus.semiconductor.com ([192.168.2.10]) by pegasus 8, 22 -- ([192.168.2.10]) with mapi; Thu, 24 Mar 2011 16:17:06 -0400 8, 22 -- From: Chris Pawlowicz {cpawlowicz-at-ubmtechinsights.com} 8, 22 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 8, 22 -- Date: Thu, 24 Mar 2011 16:17:06 -0400 8, 22 -- Subject: DIY SEM 8, 22 -- Thread-Topic: DIY SEM 8, 22 -- Thread-Index: AcvqYHH7w1yvFwuXS6uCpdColZU5VA== 8, 22 -- Message-ID: {0079587FD46DFC42AC07C9D337C7C895395FD0EF22-at-pegasus} 8, 22 -- Accept-Language: en-US 8, 22 -- Content-Language: en-US 8, 22 -- X-MS-Has-Attach: 8, 22 -- X-MS-TNEF-Correlator: 8, 22 -- acceptlanguage: en-US 8, 22 -- Content-Type: text/plain; charset="us-ascii" 8, 22 -- MIME-Version: 1.0 8, 22 -- Content-Transfer-Encoding: 8bit 8, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2OKHCUu014348 ==============================End of - Headers==============================
Strange actions like this should always be approached in the first case as a computer problem, reboot, then secondly by carrying out a complete recalibration. Your Oxford system may be totally revitalised through a complete recalibration so give that a try? Internal TV systems may also mess up a spectrum, always turn them off when carrying out an analysis.
Hope this helps?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: wzhe-at-laurentian.ca [mailto:wzhe-at-laurentian.ca] Sent: 24 March 2011 16:29 To: protrain-at-emcourses.com
Dear Listers,
We had found EDS counts suddenly dropped almost half after a couple of days no operations of our INCA/Jeol 6400 SEM system. Counts for standard materials of Ni drops from ~10k to ~5k, and noise (zero) peak goes up to about 100k compared to normal of 1-3k, we also noticed a significant “Si peak†at 1.75 KeV in spectrum of pure Ni. Is anybody had this sort of problem before or has idea what it could be? please advice. We are trying to do conditioning detector, i.e. de-ice, to see if any improvement, but still could not explain this sudden counts drop.
Any comments are highly appreciated,
Thanks,
William Zhe Central Analytical Facility Laurentian University
==============================Original Headers============================== 6, 16 -- From wzhe-at-laurentian.ca Thu Mar 24 11:26:45 2011 6, 16 -- Received: from lugwout.laurentian.ca (lugwout.laurentian.ca [142.51.1.65]) 6, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2OGQjhw026264 6, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Mar 2011 11:26:45 -0500 6, 16 -- Received: from outdom-MTA by lugwout.laurentian.ca 6, 16 -- with Novell_GroupWise; Thu, 24 Mar 2011 12:25:45 -0400 6, 16 -- Message-Id: {4D8B3872.8E8D.00C5.1-at-laurentian.ca} 6, 16 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 6, 16 -- Date: Thu, 24 Mar 2011 12:26:23 -0400 6, 16 -- From: "William Zhe" {wzhe-at-laurentian.ca} 6, 16 -- To: {Microscopy-at-microscopy.com} 6, 16 -- Subject: EDS counts problem 6, 16 -- Mime-Version: 1.0 6, 16 -- Content-Type: text/plain; charset=UTF-8 6, 16 -- Content-Transfer-Encoding: 8bit 6, 16 -- Content-Disposition: inline ==============================End of - Headers==============================
==============================Original Headers============================== 21, 28 -- From protrain-at-emcourses.com Thu Mar 24 16:59:13 2011 21, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2OLxCMR032401 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 24 Mar 2011 16:59:13 -0500 21, 28 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:51946 helo=ProtrainPC) 21, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 21, 28 -- (envelope-from {protrain-at-emcourses.com} ) 21, 28 -- id 1Q2sYg-0007mj-77 by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Thu, 24 Mar 2011 23:59:11 +0200 21, 28 -- Reply-To: {protrain-at-emcourses.com} 21, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 21, 28 -- To: {wzhe-at-laurentian.ca} 21, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 21, 28 -- References: {201103241628.p2OGSfR8027534-at-ns.microscopy.com} 21, 28 -- In-Reply-To: {201103241628.p2OGSfR8027534-at-ns.microscopy.com} 21, 28 -- Subject: RE: [Microscopy] EDS counts problem 21, 28 -- Date: Thu, 24 Mar 2011 21:58:58 -0000 21, 28 -- Organization: Protrain 21, 28 -- Message-ID: {006a01cbea6e$b18f1ec0$14ad5c40$-at-com} 21, 28 -- MIME-Version: 1.0 21, 28 -- Content-Type: text/plain; 21, 28 -- charset="utf-8" 21, 28 -- X-Mailer: Microsoft Office Outlook 12.0 21, 28 -- Thread-Index: AcvqQJZDBwOhQ4ZDQB6svzFXCNF9lgALP8KQ 21, 28 -- Content-Language: en-gb 21, 28 -- X-Scan-Signature: 3686a4f295c8701601101107079f0bff{89}} 21, 28 -- X-Trace: smtp01.dial-up.net 1Q2sYg-0007mj-77 7fe3de50dc323535060b9979b386348e 21, 28 -- Content-Transfer-Encoding: 8bit 21, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2OLxCMR032401 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] pneumatic shutter on Link ISIS EDS detector
Message: Good day all.
Question for any user currently operating a Link ISIS EDS w/pneumatic shutter.
I have an older Link ISIS detector (late 90s vintage) on a JEOL 2010 TEM that we are attempting to revive (no use for 2+ yrs). Electronics box and computer+software are apparently okay (running on a museum caliber Windows 98 machine-gotta love it). My problem (first of many I'm sure): the pneumatic shutter on the detector. When I attempt to open the shutter I get a "SHUTTER FAIL" error. I can hear the shutter move and the compressed airline is pressurized. I can move the shutter manually by physically reversing the airlines. I have not attempted to remove access plates as I have no idea what part of the shutter box system is under vacuum. Manual is not helpful. No schematics. If anyone has experience with Link ISIS systems, and might have a suggestion or idea, I would be grateful if you would pass it along. Thanks,
Tom
Thomas Williams Director, Electron Microscopy Center University of Idaho Moscow, ID
Dear list, Has anyone had experience looking at CdHgTe2 on a TEM? I expect that using a cryo stage would be the best way to inhibit contamination of the scope from the volatile components. Is this the case, and are there other methods that would allow this compound to be imaged without damage to the scope? Yours, Bill
Hi Bill, I'm not familiar with the compound, but have heard wonderful (terrible?) stories about what can happen to a high voltage power supply when Hg becomes Hg vapor and rather conductive. I would think that if the Hg is tightly bound chemically, then it shouldn't be an issue, but if you have any doubts, I think cryo might be a good bet, or at least a hedge. The biggest problem seems to be its migration after being vaporized. If you don't get rid of all of it, you'll blow up your HVPS again.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Friday, March 25, 2011 3:24 PM To: kenconverse-at-qualityimages.biz
Dear list, Has anyone had experience looking at CdHgTe2 on a TEM? I expect that using a cryo stage would be the best way to inhibit contamination of the scope from the volatile components. Is this the case, and are there other methods that would allow this compound to be imaged without damage to the scope? Yours, Bill
I would like to very sincerely thank everyone who has responded. I really got more help than I was expecting. While not quite state-of-the-art EM, this is a really meaningful project for me, and I appreciate your input.
Have a great weekend everyone, Vlad
________________________________________ X-from: Speransky, Vlad (NIH/NIBIB) [E] Sent: Wednesday, March 23, 2011 8:24 PM To: microscopy-at-microscopy.com
Bill; Your big problem will be making specimens that do not have point defects galore all over the surface. I believe Tony Cullis did a lot of CdHgTe2 work back in the 80's, and that was one of the motivators to develop iodine milling. I don't recall any problems with the compound volatilizing.
A. John Mardinly, ASU
-----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Friday, March 25, 2011 12:33 PM To: John Mardinly
Dear list, Has anyone had experience looking at CdHgTe2 on a TEM? I expect
that using a cryo stage would be the best way to inhibit contamination of the scope from the volatile components. Is this the case, and are there
other methods that would allow this compound to be imaged without damage
Dear list, Thank you to all who replied with comments on the HgCdTe imaging. The list has once again proved its worth--not that there was any doubt. Yours, Bill
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both james.m.hoffmann-at-usa.dupont.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: james.m.hoffmann-at-usa.dupont.com Name: Jim Hoffmann
Organization: E.I. Dupont de Nemours &Co., Inc
Title-Subject: [Filtered] SEM/EDS - Thank you - Table Top versus Research Grade
Message: Hello to all,
I want to thank everyone who responded to my request for insights and experiences regarding the differences between Table-Top and Research Grade SEM's. I received many really helpful comments from many wonderful subscribers that I will be reflecting on as I go forward on my new SEM adventure. Although I am mailing messages from the Internet Form due to the "attachments" problem, I am subscribed to the listserver so that anyone else who would like to comment can continue to add their two cents worth. It is much appreciated. I have not made up my mind yet what I will do. It seems there are some Pros and Cons to consider and I am reading SEM textbooks to try to understand better. For those who contacted me by E-mail, I will be getting back to you soon if you have not heard from me already. Thank you again, I am learning a lot. Yours Truly,
Jim Hoffmann, Optical Microscopist/SEM Microscopist Wannabe E.I. Dupont de Nemours & Co., Inc. E-mail: james.m.hoffmann-at-usa.dupont.com
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi all,
Before we have too much panic, it might help to remember that for many years, JEOL used a mercury diffusion pump in their tandem pumping system for their best 100 and 200 keV TEMs. Such devices intentionally produce massive amounts of mercury vapor.
Old guy comment.
Jim Pawley
*************************************************************************** Prof. James B. Pawley, Ph. 608-238-3953 21. N. Prospect Ave. Madison, WI 53726 USA JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 11-23, 2011, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2011 "If it ain't diffraction, it must be statistics." Anon.
-- *************************************************************************** Prof. James B. Pawley, Ph. 608-238-3953 21. N. Prospect Ave. Madison, WI 53726 USA JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 11-23, 2011, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2011 "If it ain't diffraction, it must be statistics." Anon.
==============================Original Headers============================== 8, 27 -- From jbpawley-at-wisc.edu Fri Mar 25 22:31:27 2011 8, 27 -- Received: from argol.doit.wisc.edu (argol.doit.wisc.edu [144.92.197.212]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2Q3VRoi020475 8, 27 -- for {microscopy-at-microscopy.com} ; Fri, 25 Mar 2011 22:31:27 -0500 8, 27 -- MIME-version: 1.0 8, 27 -- Content-transfer-encoding: 7BIT 8, 27 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 8, 27 -- Received: from avs-daemon.smtpauth3.wiscmail.wisc.edu by 8, 27 -- smtpauth3.wiscmail.wisc.edu 8, 27 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 8, 27 -- id {0LIN00M00B4FSI00-at-smtpauth3.wiscmail.wisc.edu} for 8, 27 -- microscopy-at-microscopy.com; Fri, 25 Mar 2011 22:31:27 -0500 (CDT) 8, 27 -- Received: from [192.168.1.119] 8, 27 -- (99-126-179-197.lightspeed.mdsnwi.sbcglobal.net [99.126.179.197]) 8, 27 -- by smtpauth3.wiscmail.wisc.edu 8, 27 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 8, 27 -- with ESMTPSA id {0LIN00LC7B4DSF00-at-smtpauth3.wiscmail.wisc.edu} for 8, 27 -- microscopy-at-microscopy.com; Fri, 25 Mar 2011 22:31:26 -0500 (CDT) 8, 27 -- Date: Fri, 25 Mar 2011 22:31:24 -0500 8, 27 -- From: James Pawley {jbpawley-at-wisc.edu} 8, 27 -- Subject: [Microscopy] RE: TEM of CdHgTe2 8, 27 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 8, 27 -- Message-id: {p0624080fc9b30e659801-at-[192.168.1.119]} 8, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=192.168.1.119 8, 27 -- X-Spam-PmxInfo: Server=avs-13, Version=5.6.0.2009776, 8, 27 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.3.26.32416, 8, 27 -- SenderIP=192.168.1.119 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hailstone-at-cis.rit.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I work on primarily renal biopsies for the university hospital. I have been asked to investigate, any and all techniques, to get better definition of the immune deposits that we find in the basement membrane of the glomerulus. We use a fairly standard protocol:
Fix in a 1.5% Para and 1.5% glut in a cacodylate buffer.
Post fix in OsO4 dehydrate in Acetone embed in LX-112, an epoxy plastic
Stain the thin sections with UA and Pb citrate.
We do still have the occasional very electron dense deposits in a few of our cases. But the majority are much less evident, although well defined in the fluorescent images.
Any suggestions, protocols or shared experience will be appreciated.
Robert Kayton
Oregon Health & Science University Portland, Oregon
==============================Original Headers============================== 12, 32 -- From prvs=40665BB877=kayton-at-ohsu.edu Sat Mar 26 18:39:08 2011 12, 32 -- Received: from twedge01.ohsu.edu (twedge01.ohsu.edu [137.53.6.162]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2QNd7ko011401 12, 32 -- for {microscopy-at-microscopy.com} ; Sat, 26 Mar 2011 18:39:08 -0500 12, 32 -- Received: from twapp03.ohsu.edu (unknown [10.76.7.12]) 12, 32 -- by twedge01.ohsu.edu (Axway MailGate 3.8.1) with ESMTP id 2D4E41034121 12, 32 -- for {microscopy-at-microscopy.com} ; Fri, 25 Mar 2011 16:48:42 -0700 (PDT) 12, 32 -- X-WSS-ID: 0LIN0T6-0I-N2Y-02 12, 32 -- X-M-MSG: 12, 32 -- Received: from mail.ohsu.edu (unknown [10.76.7.14]) 12, 32 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) 12, 32 -- (No client certificate requested) 12, 32 -- by twapp03.ohsu.edu (Axway MailGate 3.8.1) with ESMTP id 29D7044D985B 12, 32 -- for {microscopy-at-microscopy.com} ; Fri, 25 Mar 2011 16:48:41 -0700 (PDT) 12, 32 -- Received: from EX-MB04.ohsu.edu ([10.76.32.236]) by ex-ht04.ohsu.edu 12, 32 -- ([10.76.248.130]) with mapi; Fri, 25 Mar 2011 16:48:43 -0700 12, 32 -- From: Robert Kayton {kayton-at-ohsu.edu} 12, 32 -- To: Electron Microscopy Society Society {microscopy-at-microscopy.com} 12, 32 -- Date: Fri, 25 Mar 2011 16:48:33 -0700 12, 32 -- Subject: Help with Kidney Bx 12, 32 -- Thread-Topic: Help with Kidney Bx 12, 32 -- Thread-Index: AcvrRyw6pddBk3exQxmdto5lmE5Kog== 12, 32 -- Message-ID: {CDC23A9D-B1E7-43CE-9A1F-CCC2042CC5EC-at-ohsu.edu} 12, 32 -- Accept-Language: en-US 12, 32 -- Content-Language: en-US 12, 32 -- X-MS-Has-Attach: 12, 32 -- X-MS-TNEF-Correlator: 12, 32 -- acceptlanguage: en-US 12, 32 -- Content-Type: text/plain; charset="us-ascii" 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Transfer-Encoding: 8bit 12, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2QNd7ko011401 ==============================End of - Headers==============================
----- Original Message ---- X-from: "jbpawley-at-wisc.edu" {jbpawley-at-wisc.edu} To: nizets2-at-yahoo.com Sent: Sat, March 26, 2011 4:36:26 AM
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi all,
Before we have too much panic, it might help to remember that for many years, JEOL used a mercury diffusion pump in their tandem pumping system for their best 100 and 200 keV TEMs. Such devices intentionally produce massive amounts of mercury vapor.
Old guy comment.
Jim Pawley
*************************************************************************** Prof. James B. Pawley, Ph. 608-238-3953 21. N. Prospect Ave. Madison, WI 53726 USA JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 11-23, 2011, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2011 "If it ain't diffraction, it must be statistics." Anon.
-- *************************************************************************** Prof. James B. Pawley, Ph. 608-238-3953 21. N. Prospect Ave. Madison, WI 53726 USA JBPAWLEY-at-WISC.EDU 3D Microscopy of Living Cells Course, June 11-23, 2011, UBC, Vancouver Canada Info: http://www.3dcourse.ubc.ca/ Applications due by March 15, 2011 "If it ain't diffraction, it must be statistics." Anon.
==============================Original Headers============================== 8, 27 -- From jbpawley-at-wisc.edu Fri Mar 25 22:31:27 2011 8, 27 -- Received: from argol.doit.wisc.edu (argol.doit.wisc.edu [144.92.197.212]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2Q3VRoi020475 8, 27 -- for {microscopy-at-microscopy.com} ; Fri, 25 Mar 2011 22:31:27 -0500 8, 27 -- MIME-version: 1.0 8, 27 -- Content-transfer-encoding: 7BIT 8, 27 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 8, 27 -- Received: from avs-daemon.smtpauth3.wiscmail.wisc.edu by 8, 27 -- smtpauth3.wiscmail.wisc.edu 8, 27 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 8, 27 -- id {0LIN00M00B4FSI00-at-smtpauth3.wiscmail.wisc.edu} for 8, 27 -- microscopy-at-microscopy.com; Fri, 25 Mar 2011 22:31:27 -0500 (CDT) 8, 27 -- Received: from [192.168.1.119] 8, 27 -- (99-126-179-197.lightspeed.mdsnwi.sbcglobal.net [99.126.179.197]) 8, 27 -- by smtpauth3.wiscmail.wisc.edu 8, 27 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 8, 27 -- with ESMTPSA id {0LIN00LC7B4DSF00-at-smtpauth3.wiscmail.wisc.edu} for 8, 27 -- microscopy-at-microscopy.com; Fri, 25 Mar 2011 22:31:26 -0500 (CDT) 8, 27 -- Date: Fri, 25 Mar 2011 22:31:24 -0500 8, 27 -- From: James Pawley {jbpawley-at-wisc.edu} 8, 27 -- Subject: [Microscopy] RE: TEM of CdHgTe2 8, 27 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 8, 27 -- Message-id: {p0624080fc9b30e659801-at-[192.168.1.119]} 8, 27 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=192.168.1.119 8, 27 -- X-Spam-PmxInfo: Server=avs-13, Version=5.6.0.2009776, 8, 27 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.3.26.32416, 8, 27 -- SenderIP=192.168.1.119 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 32 -- From nizets2-at-yahoo.com Mon Mar 28 04:17:54 2011 19, 32 -- Received: from web110813.mail.gq1.yahoo.com (web110813.mail.gq1.yahoo.com [67.195.13.236]) 19, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2S9HrMS010349 19, 32 -- for {microscopy-at-microscopy.com} ; Mon, 28 Mar 2011 04:17:54 -0500 19, 32 -- Received: (qmail 41148 invoked by uid 60001); 28 Mar 2011 09:17:53 -0000 19, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1301303873; bh=OOL6s2nPqeTs++dAb4fAXXYlRBSSvtBVF9xJjL2bngQ=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=kLEyVZRzeEkHqyai+cY79UjPD2EyYv+F7OL+AtPbhY+DGScsOuWWbzTROtOE69cUHGEmcbaIEzKUUBLIsUaMNa5e3T83H9642YSMNT8HAoxIPfcJFS5BzDbCbvtC13Psce4xgjoiblXt0JjerLFUxvQg3g0SVSwoeYEASgtuCto= 19, 32 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 19, 32 -- s=s1024; d=yahoo.com; 19, 32 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:Cc:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 19, 32 -- b=kiFJ+XlH0Np0SZQFN8JJtx5uVmiuUTlp7nNAnVLVSi6Iww31Zzh0tI9R3W5SAItYFojc6LjRY7UH9F7nmYQromBGCuqoA9ttwnuoJkwXdRt4lIU4LBieb2lnymMqoQwoAzf3XPtgxNIYyv+FCCB0L99jcrWcSjVghF3hgwFn94U=; 19, 32 -- Message-ID: {329539.29785.qm-at-web110813.mail.gq1.yahoo.com} 19, 32 -- X-YMail-OSG: lM_sbssVM1nNOl280IvZgVnjo7fxMOFF8j2n8Lby9NBPKWd 19, 32 -- boBPZooFiHltY0v5RWUsezhWr.cz02ttSjmmH6sKIr36CcDdGuq53WsgVKk2 19, 32 -- piLaCcXhxD1VqrvTuBkrW5Y2yOY.MMBVS91rnEb_LgLD0EdYAepzuRad_XWS 19, 32 -- POet1jJPfaveB0_LjZVwhVBAly.izQpPKI7uL8wZrnlzK_g..VIWm5BHn0yB 19, 32 -- jhDLgd5JRGZ4PIo4j2b2eTR4kN1RCVZv1WQuszl44S0WxZLsByA5NCpHbqqe 19, 32 -- ZZVs.T2Z6NkGetCs7zMuoygrhNcUX86juMh4HAB9J6f7i5TMAEDikkpzDeEh 19, 32 -- GW8LBroba5yqXr7gwhb3WLC.FlKSgSOiQKc7UsTMCvcTPExrlZKZIj16R5A_ 19, 32 -- hVzdx1IP7XBKHzzb4 19, 32 -- Received: from [80.122.101.100] by web110813.mail.gq1.yahoo.com via HTTP; Mon, 28 Mar 2011 02:17:53 PDT 19, 32 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 19, 32 -- References: {201103260336.p2Q3aQIA026288-at-ns.microscopy.com} 19, 32 -- Date: Mon, 28 Mar 2011 02:17:53 -0700 (PDT) 19, 32 -- From: Stephane Nizet {nizets2-at-yahoo.com} 19, 32 -- Subject: Re: [Microscopy] RE: TEM of CdHgTe2 19, 32 -- To: jbpawley-at-wisc.edu 19, 32 -- Cc: microscopy-at-microscopy.com 19, 32 -- In-Reply-To: {201103260336.p2Q3aQIA026288-at-ns.microscopy.com} 19, 32 -- MIME-Version: 1.0 19, 32 -- Content-Type: text/plain; charset=iso-8859-1 19, 32 -- Content-Transfer-Encoding: 8bit 19, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2S9HrMS010349 ==============================End of - Headers==============================
Dear All, our lab has a JEOL Vacuum Evaporator JEE-400 all a long but its not frequently used. Recently i am trying to make use of this evaporator to establish a consistent carbon coating of certain thickness. Unfortunately there are no expertise in our lab and i would to ask for some advice particularly on the sharpness of the carbon rod. Below is my question:
How sharp must the carbon rod be for the contact? The JEOL manual illustrate a needle sharp tip but the JEOL carbon sharpener seem only able to thin the rod to a diameter of about 1mm. Would that actually means that after using the JEOL carbon sharpener, i should find other ways to create a sharp tip?
I appreciate very much for your kind advice!
Best Regards, Yee Yan, Tay NTU, Singapore
==============================Original Headers============================== 5, 27 -- From one_twinklestar-at-yahoo.com.sg Mon Mar 28 12:25:07 2011 5, 27 -- Received: from web77408.mail.sg1.yahoo.com (web77408.mail.sg1.yahoo.com [183.177.84.71]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2SHP6F6005876 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Mar 2011 12:25:07 -0500 5, 27 -- Received: (qmail 36710 invoked by uid 60001); 28 Mar 2011 17:25:05 -0000 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1301333105; bh=e0E1C82AHABEwZLmG9QUzr71vdadRTNARK0pKHwQZ5c=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=kk+NvEuy4znEdSv5ZOSrxQh8xtXimywzhgZoL9igsJIpMiDrU9b3Jp1NpagGh69lOfHnQvGgVeDOPbdc/XP8V6TfQoRK/7octI+p5L6e/Mx17snub+adgo//tFfyM7OHGXguuEv99rn+SX5U7gVqsbjtJ91q6zHz4fMg/hg+uDA= 5, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 27 -- s=s1024; d=yahoo.com.sg; 5, 27 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 27 -- b=yUrKZ78vzEQiRAFKCUnImDP3jAi01tRq0T4CFZTcv8xyf8ojDsDIKiih83RwL+UOXJ/knP478ImdkMKuiqzyiRI6DgoZtQjUHSkNPZzuH5BonplM6EDGKXiDvxv0yHNFjIdSFYlH0Y0V1/gfCLNT/0HQYmA9JFp4KP6aTfTKOfQ=; 5, 27 -- Message-ID: {137029.34853.qm-at-web77408.mail.sg1.yahoo.com} 5, 27 -- X-YMail-OSG: fjIj.7AVM1lrCvvMO3jycvfvubbyxb10Ae0SPKe1C5dF7Ml 5, 27 -- _z7B3NzircnUU8nMUTRT4v97BAyuU19bMilPGD_9yF6mzAg6EqQ1mrHgAKnj 5, 27 -- nAQCOdS7GmehXs66p.aULLYEL2PF.3ju3cHFDjcBjgqye84KSxRn.dcaPa3x 5, 27 -- FZejMz5UVgk2HbB7CzbVcwUScJslb8hYuM0ZpiiJNkY4IJECcYgeMKpZblf0 5, 27 -- .yYgWWsRvp0jMThHZwSN031NAZdhT2FijGZ77S4O7mokbJCx00ZuaAOK_te7 5, 27 -- enhfb97Un5XScbEOXJnk- 5, 27 -- Received: from [121.6.38.123] by web77408.mail.sg1.yahoo.com via HTTP; Tue, 29 Mar 2011 01:25:04 SGT 5, 27 -- X-Mailer: YahooMailClassic/12.0.2 YahooMailWebService/0.8.109.295617 5, 27 -- Date: Tue, 29 Mar 2011 01:25:04 +0800 (SGT) 5, 27 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 5, 27 -- Subject: Carbon Rod used in Carbon Evaporator 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=utf-8 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2SHP6F6005876 ==============================End of - Headers==============================
My own experience with a Cressington evaporator is that too fine a tip leads to unreliable evaporation - I spent a good deal of time getting the tip pencil sharp only to discover that it would spark up briefly then break before sufficient carbon had evaporated. A 1mm works well, I get control of the thickness by donning a pair of welding goggles and watching the arc light up, for general purpose grids I allow about a 2 second pulse from the point at which the arc reaches maximum brightness.
Cheers Ian
Ian Portman Imaging Suite Manager School of Life Sciences University of Warwick
------------------------------------------------------------------------ ---- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear All, our lab has a JEOL Vacuum Evaporator JEE-400 all a long but its not frequently used. Recently i am trying to make use of this evaporator to establish a consistent carbon coating of certain thickness. Unfortunately there are no expertise in our lab and i would to ask for some advice particularly on the sharpness of the carbon rod. Below is my question:
How sharp must the carbon rod be for the contact? The JEOL manual illustrate a needle sharp tip but the JEOL carbon sharpener seem only able to thin the rod to a diameter of about 1mm. Would that actually means that after using the JEOL carbon sharpener, i should find other ways to create a sharp tip?
I appreciate very much for your kind advice!
Best Regards, Yee Yan, Tay NTU, Singapore
==============================Original Headers============================== 15, 37 -- From I.J.Portman-at-warwick.ac.uk Tue Mar 29 02:32:39 2011 15, 37 -- Received: from mail-relay-2.csv.warwick.ac.uk (mail-relay-2.csv.warwick.ac.uk [137.205.128.8]) 15, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2T7WcY9012989 15, 37 -- for {microscopy-at-microscopy.com} ; Tue, 29 Mar 2011 02:32:38 -0500 15, 37 -- Received: from localhost (localhost [127.0.0.1]) 15, 37 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p2T7Wb0F001501; 15, 37 -- Tue, 29 Mar 2011 08:32:37 +0100 (BST) 15, 37 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 15, 37 -- Received: from mail-relay-2.csv.warwick.ac.uk ([127.0.0.1]) 15, 37 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 15, 37 -- with LMTP id hPI3bMltXyjK; Tue, 29 Mar 2011 08:32:37 +0100 (BST) 15, 37 -- Received: from beech.ads.warwick.ac.uk (beech [137.205.97.163]) 15, 37 -- by mail-relay-2.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p2T7WaSE001456; 15, 37 -- Tue, 29 Mar 2011 08:32:36 +0100 (BST) 15, 37 -- X-Envelope-From: I.J.Portman-at-warwick.ac.uk 15, 37 -- Received: from ELDER.ads.warwick.ac.uk ([137.205.97.181]) by beech.ads.warwick.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 15, 37 -- Tue, 29 Mar 2011 08:32:35 +0100 15, 37 -- MIME-Version: 1.0 15, 37 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 37 -- Content-class: urn:content-classes:message 15, 37 -- Content-Type: text/plain; 15, 37 -- charset="us-ascii" 15, 37 -- Subject: RE: [Microscopy] Carbon Rod used in Carbon Evaporator 15, 37 -- Date: Tue, 29 Mar 2011 08:32:35 +0100 15, 37 -- Message-ID: {DB126BA68A8A5A439BAF072438A39FEA0294DB89-at-ELDER.ads.warwick.ac.uk} 15, 37 -- In-Reply-To: {201103281732.p2SHWoDa016615-at-ns.microscopy.com} 15, 37 -- X-MS-Has-Attach: 15, 37 -- X-MS-TNEF-Correlator: 15, 37 -- Thread-Topic: [Microscopy] Carbon Rod used in Carbon Evaporator 15, 37 -- Thread-Index: AcvtbjpewvgsDsBlSLaQxmHjwcCEiwAc/0VA 15, 37 -- References: {201103281732.p2SHWoDa016615-at-ns.microscopy.com} 15, 37 -- From: "Portman, Ian" {I.J.Portman-at-warwick.ac.uk} 15, 37 -- To: {one_twinklestar-at-yahoo.com.sg} , {microscopy-at-microscopy.com} 15, 37 -- X-OriginalArrivalTime: 29 Mar 2011 07:32:35.0530 (UTC) FILETIME=[791A0AA0:01CBEDE3] 15, 37 -- X-DCC-Warwick-Metrics: nightshade; whitelist 15, 37 -- Content-Transfer-Encoding: 8bit 15, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2T7WcY9012989 ==============================End of - Headers==============================
We used to form two wedges by moving the rod across a grinding paper. The wedge angle was approx. 30-45 degrees. We also had a rotation vacuum transfer rod, which allowed us to press one wedge against the other (stationary) one.
Joe
==============================Original Headers============================== 4, 23 -- From Josef.Zweck-at-physik.uni-regensburg.de Tue Mar 29 03:21:05 2011 4, 23 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2T8L4dP029783 4, 23 -- for {microscopy-at-microscopy.com} ; Tue, 29 Mar 2011 03:21:05 -0500 4, 23 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 4, 23 -- by localhost (Postfix) with SMTP id E79CB37C6 4, 23 -- for {microscopy-at-microscopy.com} ; Tue, 29 Mar 2011 10:20:59 +0200 (CEST) 4, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 4, 23 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id D9D183753 4, 23 -- for {microscopy-at-microscopy.com} ; Tue, 29 Mar 2011 10:20:59 +0200 (CEST) 4, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 4, 23 -- with Novell_GroupWise; Tue, 29 Mar 2011 10:21:00 +0200 4, 23 -- Message-Id: {4D91B2890200004000011B63-at-gwsmtp1.uni-regensburg.de} 4, 23 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 4, 23 -- Date: Tue, 29 Mar 2011 10:20:57 +0200 4, 23 -- From: "Josef Zweck" {Josef.Zweck-at-physik.uni-regensburg.de} 4, 23 -- To: "Listserver Microscopy" {microscopy-at-microscopy.com} 4, 23 -- Subject: RE: Carbon Rod used in Carbon Evaporator 4, 23 -- Mime-Version: 1.0 4, 23 -- Content-Type: text/plain; charset=US-ASCII 4, 23 -- Content-Disposition: inline 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2T8L4dP029783 ==============================End of - Headers==============================
Dear All, thank you so much for your valuable suggestion. Reading through your suggestions, it gives me a lot of idea what i can do with my carbon evaporator as well as refining the technique to give a fine controlled coating!!!
Thanks everyone!!
Cheers, Yee Yan, Tay NTU
--- On Tue, 29/3/11, Josef.Zweck-at-physik.uni-regensburg.de {Josef.Zweck-at-physik.uni-regensburg.de} wrote:
} From: Josef.Zweck-at-physik.uni-regensburg.de {Josef.Zweck-at-physik.uni-regensburg.de} } Subject: [Microscopy] RE: Carbon Rod used in Carbon Evaporator } To: one_twinklestar-at-yahoo.com.sg } Date: Tuesday, 29 March, 2011, 4:22 PM } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We used to form two wedges by moving the rod across a } grinding paper. The wedge angle was approx. 30-45 degrees. } We also had a rotation vacuum transfer rod, which allowed us } to press one wedge against the other (stationary) one. } } Joe } } } } ==============================Original } Headers============================== } 4, 23 -- From Josef.Zweck-at-physik.uni-regensburg.de } Tue Mar 29 03:21:05 2011 } 4, 23 -- Received: from rrzmta2.uni-regensburg.de } (rrzmta2.uni-regensburg.de [194.94.155.52]) } 4, 23 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id p2T8L4dP029783 } 4, 23 -- for {microscopy-at-microscopy.com} ; } Tue, 29 Mar 2011 03:21:05 -0500 } 4, 23 -- Received: from rrzmta2.uni-regensburg.de } (localhost [127.0.0.1]) } 4, 23 -- by localhost (Postfix) with
The ideal carbon rod combination is a spigot and a flat which is exactly what your JEOL sharpener is providing for you. The spigot system is better than a point system as it provides a possibility of prolonged coating due to the constant surface area of the contact point. A point system very soon erodes to a surface area that is too large for the evaporation to continue
Set the spigot to strike a flat surface with the sprung pressure being applied to the spigot rod. Remember the quality of a carbon coat is directly related to the vacuum level when setting up the system; give it time!
Good luck!
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg] Sent: 28 March 2011 18:26 To: protrain-at-emcourses.com
Dear All, our lab has a JEOL Vacuum Evaporator JEE-400 all a long but its not frequently used. Recently i am trying to make use of this evaporator to establish a consistent carbon coating of certain thickness. Unfortunately there are no expertise in our lab and i would to ask for some advice particularly on the sharpness of the carbon rod. Below is my question:
How sharp must the carbon rod be for the contact? The JEOL manual illustrate a needle sharp tip but the JEOL carbon sharpener seem only able to thin the rod to a diameter of about 1mm. Would that actually means that after using the JEOL carbon sharpener, i should find other ways to create a sharp tip?
I appreciate very much for your kind advice!
Best Regards, Yee Yan, Tay NTU, Singapore
==============================Original Headers============================== 5, 27 -- From one_twinklestar-at-yahoo.com.sg Mon Mar 28 12:25:07 2011 5, 27 -- Received: from web77408.mail.sg1.yahoo.com (web77408.mail.sg1.yahoo.com [183.177.84.71]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2SHP6F6005876 5, 27 -- for {microscopy-at-microscopy.com} ; Mon, 28 Mar 2011 12:25:07 -0500 5, 27 -- Received: (qmail 36710 invoked by uid 60001); 28 Mar 2011 17:25:05 -0000 5, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1301333105; bh=e0E1C82AHABEwZLmG9QUzr71vdadRTNARK0pKHwQZ5c=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version :Content-Type:Content-Transfer-Encoding; b=kk+NvEuy4znEdSv5ZOSrxQh8xtXimywzhgZoL9igsJIpMiDrU9b3Jp1NpagGh69lOfHnQvGgVe DOPbdc/XP8V6TfQoRK/7octI+p5L6e/Mx17snub+adgo//tFfyM7OHGXguuEv99rn+SX5U7gVqsb jtJ91q6zHz4fMg/hg+uDA= 5, 27 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 27 -- s=s1024; d=yahoo.com.sg; 5, 27 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version :Content-Type:Content-Transfer-Encoding; 5, 27 -- b=yUrKZ78vzEQiRAFKCUnImDP3jAi01tRq0T4CFZTcv8xyf8ojDsDIKiih83RwL+UOXJ/knP478I mdkMKuiqzyiRI6DgoZtQjUHSkNPZzuH5BonplM6EDGKXiDvxv0yHNFjIdSFYlH0Y0V1/gfCLNT/0 HQYmA9JFp4KP6aTfTKOfQ=; 5, 27 -- Message-ID: {137029.34853.qm-at-web77408.mail.sg1.yahoo.com} 5, 27 -- X-YMail-OSG: fjIj.7AVM1lrCvvMO3jycvfvubbyxb10Ae0SPKe1C5dF7Ml 5, 27 -- _z7B3NzircnUU8nMUTRT4v97BAyuU19bMilPGD_9yF6mzAg6EqQ1mrHgAKnj 5, 27 -- nAQCOdS7GmehXs66p.aULLYEL2PF.3ju3cHFDjcBjgqye84KSxRn.dcaPa3x 5, 27 -- FZejMz5UVgk2HbB7CzbVcwUScJslb8hYuM0ZpiiJNkY4IJECcYgeMKpZblf0 5, 27 -- .yYgWWsRvp0jMThHZwSN031NAZdhT2FijGZ77S4O7mokbJCx00ZuaAOK_te7 5, 27 -- enhfb97Un5XScbEOXJnk- 5, 27 -- Received: from [121.6.38.123] by web77408.mail.sg1.yahoo.com via HTTP; Tue, 29 Mar 2011 01:25:04 SGT 5, 27 -- X-Mailer: YahooMailClassic/12.0.2 YahooMailWebService/0.8.109.295617 5, 27 -- Date: Tue, 29 Mar 2011 01:25:04 +0800 (SGT) 5, 27 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 5, 27 -- Subject: Carbon Rod used in Carbon Evaporator 5, 27 -- To: microscopy-at-microscopy.com 5, 27 -- MIME-Version: 1.0 5, 27 -- Content-Type: text/plain; charset=utf-8 5, 27 -- Content-Transfer-Encoding: 8bit 5, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2SHP6F6005876 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 27 -- From protrain-at-emcourses.com Tue Mar 29 12:42:58 2011 18, 27 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 18, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2THgvAB028464 18, 27 -- for {microscopy-at-microscopy.com} ; Tue, 29 Mar 2011 12:42:58 -0500 18, 27 -- Received: from 5ac8bff6.bb.sky.com ([90.200.191.246]:50794 helo=ProtrainPC) 18, 27 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 18, 27 -- (envelope-from {protrain-at-emcourses.com} ) 18, 27 -- id 1Q4cwR-000Ceu-25 by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Tue, 29 Mar 2011 19:42:55 +0200 18, 27 -- Reply-To: {protrain-at-emcourses.com} 18, 27 -- From: "Steve Chapman" {protrain-at-emcourses.com} 18, 27 -- To: {one_twinklestar-at-yahoo.com.sg} 18, 27 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 18, 27 -- References: {201103281726.p2SHQKYm007121-at-ns.microscopy.com} 18, 27 -- In-Reply-To: {201103281726.p2SHQKYm007121-at-ns.microscopy.com} 18, 27 -- Subject: RE: [Microscopy] Carbon Rod used in Carbon Evaporator 18, 27 -- Date: Tue, 29 Mar 2011 18:42:20 +0100 18, 27 -- Organization: Protrain 18, 27 -- Message-ID: {003c01cbee38$b867f0f0$2937d2d0$-at-com} 18, 27 -- MIME-Version: 1.0 18, 27 -- Content-Type: text/plain; 18, 27 -- charset="us-ascii" 18, 27 -- Content-Transfer-Encoding: 7bit 18, 27 -- X-Mailer: Microsoft Office Outlook 12.0 18, 27 -- Thread-Index: AcvtbU48pHploViLQLab5xGfdj+bEQAyGM6g 18, 27 -- Content-Language: en-gb 18, 27 -- X-Scan-Signature: 4324ba97ba8ec510c2abd56a77ecfd73{106}} 18, 27 -- X-Trace: smtp01.dial-up.net 1Q4cwR-000Ceu-25 9e5e7d68ceabcd7b92a05b05f8079dcf ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both iclements-at-api.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: iclements-at-api.com Name: Ian Clements
Organization: Applied Precision Inc
Title-Subject: [Filtered] Job Opening - Bio Service Engineer
Message: Dear Listmembers
We are currently recruiting for an experienced Bio Engineer/Service Engineer to join our DeltaVision OMX super-resolution microscope support team. The position of Bio Engineer is part of the Life Sciences Service Team. Under limited supervision, provides installation, training and advanced technical and applications support for API end customers and distributors. For more details follow the link below.
This is the direct link to the job profile. https://www.recruitingcenter.net/Clients/appliedprecision/PublicJobs/controller.cfm?jbaction=JobProfile&job_id=10033
If you experience problems using the link you can also access this and other open positions through our website at http://www.appliedprecision.com/current-openings.asp
Ian Clements Product Manager - DeltaVision OMX Systems
I have been trying to label the CEA factor in MCF-7 cells in culture by IF without success. I can measure CEA in whole cell extracts by ELISA using a kit so its presence is certain. Unfortunately the company does not sell the antibody used in the kit so I had to buy another primary antibody from another source (Mab H-8 from Santy Cruz). The secondary is a brand new antibody so it is not the culprit. My problem is that by IF I get no specific signal, just low background signal. It may be that the primary simply doesn't work but before I resign I would like to ask if someone may had an idea how to reveal the antigen better. I expect the antigen to be bound to the cell membrane.
Here is my method: - fixation for 15 min with 4% PFA in 0,1M phosphate buffer - permeabilization for 5 min (or not, I try both in parallel) with 0,01% triton x-100/PBS - blocking 30 min with 1% BSA/PBS - 1h30 at 37°C with anti-CEA Mab H-8 1/50 in 0,5% BSA/PBS - 1h30 at 37°C with GAM-alexa 488 1/100 in 0,5% BSA/PBS
Thank you in advance
Stephane
==============================Original Headers============================== 7, 34 -- From nizets2-at-yahoo.com Wed Mar 30 01:56:47 2011 7, 34 -- Received: from nm23.bullet.mail.ne1.yahoo.com (nm23.bullet.mail.ne1.yahoo.com [98.138.90.86]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2U6ulct015633 7, 34 -- for {microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 01:56:47 -0500 7, 34 -- Received: from [98.138.90.56] by nm23.bullet.mail.ne1.yahoo.com with NNFMP; 30 Mar 2011 06:56:47 -0000 7, 34 -- Received: from [98.138.87.6] by tm9.bullet.mail.ne1.yahoo.com with NNFMP; 30 Mar 2011 06:56:47 -0000 7, 34 -- Received: from [127.0.0.1] by omp1006.mail.ne1.yahoo.com with NNFMP; 30 Mar 2011 06:56:46 -0000 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-Yahoo-Newman-Id: 985325.97781.bm-at-omp1006.mail.ne1.yahoo.com 7, 34 -- Received: (qmail 29678 invoked by uid 60001); 30 Mar 2011 06:56:46 -0000 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1301468206; bh=RFU8Nt2hi3pPZvRSp8/jNlna+o93raOIuSQFhmgndUc=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=mMCtGkwcZFX+lTAa1Nf2+VBf3ASWKQt8jPhxic2BWIfA71nvGlvLP8GeOWcIPU3wCdIehF3pK0IBENBsvnkWqZMCD1Bz5tLIIqKNN82fgBlT+tf+gNJ3+SVh8SUG2kz3Trt85JsFes3zv2hRlI2lOdYMKmNdsvUDv/nPilm8BeY= 7, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 34 -- s=s1024; d=yahoo.com; 7, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 34 -- b=Iar9AFrzOMq+x10ySWtRYHLkIZQ1IxpVIlSNUmZBwaxhxuao7UCuogo1a20ebdgxpD+s9S732OdjnbiB+kC5q6lkDlnTL+ifYdgbdlMTROFzjxdrA9TWi1uOSE+xkEIqLaFLL0B104Z6pos8TwUFo7xgiIU+PRTFfpHBZUQAe7g=; 7, 34 -- Message-ID: {47752.97466.qm-at-web110810.mail.gq1.yahoo.com} 7, 34 -- X-YMail-OSG: Cn4sOPMVM1k0h5I.IaEyNZV_SKpjlMcwmohctvBWtURIKSJ 7, 34 -- UJ7o6nKRQdy.x0Dz36Pn65Zw36HEyQl2_vPqRN15bNGjzfsQqct4YVhaeFpB 7, 34 -- Xn_GWeWIQitiDmpAqjvzDLj1S2ML6l7oFkTChEFRDm4fy_FIB8OgFdSKSsNo 7, 34 -- eVy.uCw6sijLQek9ekR18DorjKqArqmAAAN0KMyCLOZzCrFMwWby0HEbGa2D 7, 34 -- W02U37RnAZio3SXgACUfgPLSFaul62zyDepNSQxyvndBqyoAYdB58IDP.Q5b 7, 34 -- 8Lr6zvuB_wjrZb4fNg2nlUVgz9a_t1vcG766_67R2HKs0Umkh1ej.kv93exK 7, 34 -- _T0spTt0BBk7T7c477jWou3k3fwG9abjxPpRoQO.kYT5PuYPUJa.RnbLSXpq 7, 34 -- Cf73t4n6jXtk8wA-- 7, 34 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Tue, 29 Mar 2011 23:56:45 PDT 7, 34 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 7, 34 -- Date: Tue, 29 Mar 2011 23:56:45 -0700 (PDT) 7, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 34 -- Subject: Advice for IF labeling 7, 34 -- To: microscopy-at-microscopy.com 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; charset=iso-8859-1 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2U6ulct015633 ==============================End of - Headers==============================
Dear James I think all the right things were mention. I love the table top idea but from your description you will need a low end SEM with SE, BSE and EDS to handle the samples. A Tungsten filament instrument is getting relatively cheap and will do the trick, a Carbon coater and Au/Pd Coater will be needed for sample preparation.
The bit missing is my petty bit, service. Before you consider a sem just make sure the service in your area is good. The best sem is the one that is running all the time and that often depends on the service department of the representer in your area. We have a Philips XL30 ESEM and they do not produce them anymore but our downtime is virtually none. A combination of a good microscope and good service on a regular bases.
Regards Stephan H Coetzee EM Scientist University of Botswana
On Thu, Mar 17, 2011 at 3:25 PM, {cpawlowicz-at-ubmtechinsights.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } James, } } We have a busy lab with a number of microscopes (optical, SEM, TEM etc). While most of our SEMs are full size systems (Zeiss, JEOL), some with EDS, we also have a tabletop Hitachi TM1000 SEM. I also recently had a TM3000 with EDS in as a demo unit to try out and compare. (it's handy to have a small fast SEM right in the lab area). } } Most of our work is semiconductor based. } } First off, we are impressed with the TM1000 (and even more impressed with the TM3000) in how quickly and easily it can be used to get great images (albeit limited in mag). It definitely has limited controls (compared to a full size system) but you can learn how to use it in about 30 seconds. It is much harder for people to screw it up also! The EDS was quick and intuitive to learn how to use and had fast mapping capabilities. EDS capability was similar to what we have on our full size SEM (by no means 'dumbed down'). } } Pros: } Fast, easy to use (for trained and non-trained people alike). } Excellent quality images. } Various 'high pressure' modes meaning you often don't need to coat. } No special services required (water, special power, air -plug it in to standard outlet!) and small size (really is table-top). } Easy to exchange filaments } } Cons: } Limited magnification } BSE detector only (no SE) } Limited beam voltages (have choice of a few fixed steps) } Small stage } } Pricing was a bit hard to pin down.. base SEM was ~$80k, add another ~$75k for EDS. Other options like motorized stage are available. We were looking at the demo unit (year end sale) and price was about 25% discount. } } I have seen TM1000s on the used market for ~$10k or less with no EDS. } } Definitely 'try before you buy' - and one thing I'm still not sure about is the stated 'magnification'. } We have our SEMS mostly set to use Polaroid output as the reference for mag but I'm not sure what Hitachi uses. To get the same image, 10,000x on the Hitachi = 3,000x on our Zeiss and JEOL.. confusing! } } Finally, our TM1000 has been very reliable except for the turbo.. it only lasted a year or so before making bad noises. Hitachi replaced it no problems but I don't know if this is a problem area or not. The TM3000 uses a new turbo. } } Chris Pawlowicz, B.Eng } Manager, Lab Development } UBM TechInsights } 3000 Solandt Rd Ottawa ON Canada K2K 2X2 } T: +1 613 576 0150 } F: +1 613 599 6501 } E: cpawlowicz-at-ubmtechinsights.com } } } } --------------------------------------------------------------------------- } } Email: james.m.hoffmann-at-usa.dupont.com Name: Jim Hoffmann } } Organization: E.I. Dupont } } Title-Subject: [Filtered] SEM - Table Top SEM versus Research Grade SEM } } Message: Hello Listserver Folks, } } I am seeking your advice and experience. I am an Optical Microscopist working at a manufacturing site in a busy microscopy lab. The type of samples that I see cover a wide range including: failure analysis, customer support, small particle identification, R&D support, manufacturing issues, materials analysis, defect identification, root cause troubleshooting, Quality Control, polymers, organics, inorganics, etc... Besides Optical microscopy I also use Micro FTIR spectroscopy. I would like to add an SEM to my lab primarily so that I can get EDS elemental information to complement the FTIR information. The increased magnification and depth of field beyond optical microscopy would be an added bonus but not the critical need. I usually don't need over 1000x, but occasionally I need to go to 10,000 - 20,000x. For this I send samples out for SEM. } I was wondering if any of you folks have insights or experience with desk-top SEM's such as EVEX Mini-SEM, Aspex Express/Explorer, or Hitachi TM3000 ? Since I am not a trained SEM microscopist, I am thinking that the table-top models would be just right for me ... easier to operate with less training ... easier maintenance ... no sputter coating } required ... What would I be giving up in terms of EDS capability and } Imaging capability? Also, since I am at a manufacturing facility, funds are tight in my lab. I am thinking I may need to go with a "previously owned" instrument... I am not sure how much I have to spend at this point .... } } Thank } } Login Host: 52.129.8.48 } --------------------------------------------------------------------------- } } CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation. } } } ==============================Original Headers============================== } 23, 27 -- From cpawlowicz-at-ubmtechinsights.com Thu Mar 17 08:18:02 2011 } 23, 27 -- Received: from pegasus.semiconductor.com (semi-fw.ubmtechinsights.com [207.61.67.10]) } 23, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2HDI1Id011580 } 23, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Mar 2011 08:18:01 -0500 } 23, 27 -- Received: from pegasus.semiconductor.com ([192.168.2.10]) by pegasus } 23, 27 -- ([192.168.2.10]) with mapi; Thu, 17 Mar 2011 09:17:29 -0400 } 23, 27 -- From: Chris Pawlowicz {cpawlowicz-at-ubmtechinsights.com} } 23, 27 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 27 -- CC: "'james.m.hoffmann-at-usa.dupont.com'" {james.m.hoffmann-at-usa.dupont.com} } 23, 27 -- Date: Thu, 17 Mar 2011 09:17:28 -0400 } 23, 27 -- Subject: RE: [Microscopy] viaWWW:SEM - Table Top SEM versus Research Grade } 23, 27 -- SEM } 23, 27 -- Thread-Topic: [Microscopy] viaWWW:SEM - Table Top SEM versus Research Grade } 23, 27 -- SEM } 23, 27 -- Thread-Index: AcvkNfCxPllp39yhQwayVvBwf/2rNwAad+2A } 23, 27 -- Message-ID: {0079587FD46DFC42AC07C9D337C7C895395FD0ED5B-at-pegasus} } 23, 27 -- References: {201103162358.p2GNwCVx024150-at-ns.microscopy.com} } 23, 27 -- In-Reply-To: {201103162358.p2GNwCVx024150-at-ns.microscopy.com} } 23, 27 -- Accept-Language: en-US } 23, 27 -- Content-Language: en-US } 23, 27 -- X-MS-Has-Attach: } 23, 27 -- X-MS-TNEF-Correlator: } 23, 27 -- acceptlanguage: en-US } 23, 27 -- Content-Type: text/plain; charset="us-ascii" } 23, 27 -- MIME-Version: 1.0 } 23, 27 -- Content-Transfer-Encoding: 8bit } 23, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2HDI1Id011580 } ==============================End of - Headers============================== }
-- Stephan H Coetzee EM Scientist Electron Microscope Unit University of Botswana
==============================Original Headers============================== 9, 35 -- From s.h.coetzee-at-gmail.com Wed Mar 30 07:48:12 2011 9, 35 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) 9, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2UCmCcc006804 9, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 07:48:12 -0500 9, 35 -- Received: by wyf19 with SMTP id 19so1244040wyf.0 9, 35 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 05:48:11 -0700 (PDT) 9, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 35 -- d=gmail.com; s=gamma; 9, 35 -- h=domainkey-signature:mime-version:in-reply-to:references:date 9, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 9, 35 -- bh=7+7uM+O8O0lPw1uoF2yRbYXvb/mzGOmL8CGUgbNO+Z0=; 9, 35 -- b=OdbnyP3e9IpSBGqffA9Zi8rzapYqGzbCtFr7wwGOr1cpEi5erSflU5iA1kExNqMpRp 9, 35 -- zrX8vwQfk3vdqdaVdWK2oFfD6DID2dsoX9X/rKStsdGn5EpMopG2jE8ecmNWCJxJvxXK 9, 35 -- c2ybjJhZKRROz59y2I3TiyqOxOctotCqyvGC8= 9, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 9, 35 -- d=gmail.com; s=gamma; 9, 35 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 9, 35 -- :content-type:content-transfer-encoding; 9, 35 -- b=p9HJ2d3oWYRepyazQ7BCN6WxNmASbTEwGuskkxf0KsnvjG5bzR9El6mAaqtzUx0ktl 9, 35 -- Jcgw7mlN9kMwsMfgVBQGbUAfT3rD5/bkrgmaapN0F709flfqHs/+it5Cf5Se9dPoLsrK 9, 35 -- 0eLfYxJXesnYSY3OTG4+txS2IqdvKmofZ/MWo= 9, 35 -- MIME-Version: 1.0 9, 35 -- Received: by 10.216.142.199 with SMTP id i49mr1297905wej.33.1301489290238; 9, 35 -- Wed, 30 Mar 2011 05:48:10 -0700 (PDT) 9, 35 -- Received: by 10.216.68.13 with HTTP; Wed, 30 Mar 2011 05:48:10 -0700 (PDT) 9, 35 -- In-Reply-To: {201103171325.p2HDPNc7021096-at-ns.microscopy.com} 9, 35 -- References: {201103171325.p2HDPNc7021096-at-ns.microscopy.com} 9, 35 -- Date: Wed, 30 Mar 2011 14:48:10 +0200 9, 35 -- Message-ID: {AANLkTikfdTqkJPQjLp7Us=2pm2cx_8kWjmYAQVizCGPA-at-mail.gmail.com} 9, 35 -- Subject: Re: [Microscopy] RE: viaWWW:SEM - Table Top SEM versus Research Grade 9, 35 -- From: Stephan Coetzee {s.h.coetzee-at-gmail.com} 9, 35 -- To: microscopy {Microscopy-at-microscopy.com} 9, 35 -- Content-Type: text/plain; charset=ISO-8859-1 9, 35 -- Content-Transfer-Encoding: 8bit 9, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2UCmCcc006804 ==============================End of - Headers==============================
It you are looking for surface expressed CEA, there is no need to permeabilize. Even if you want both surface and internal CEA, try it without permeabilization one time to see if that helps. If you suspect the epitope is being masked by formaldehyde fixation, you could try an acetone fixation to see if it survives that. The morphology isn't as good but it getting to a positive starting point is a good first step. 90 min for primary incubation is short. We routinely do overnight. For some antibodies this is overkill and can increase background but our experience is that it often helps and less often hurts. Once again, getting a positive starting point is a good first step. We routinely use an antigen retrieval step with our paraffin and resin embedded sections - usually 10-20 min in 100 mM glycine (pH 9.6) at 95 C and see a dramatic increase in success rate. Having a new secondary is not a guarantee of success. If you buy a highly cross-reacted secondary (e.g., goat anti-mouse cross-absorbed against rat, rabbit, human, donkey, guinea pig), you might reduce the binding so some subclasses of mouse immunoglobulins (e.g., IgG2a). Is your monoclonal an IgG or IgM? IgM antibodies are less desirable for immunocytochemistry, especially internal epitopes, since they are so much larger. You don't list a concentration of monoclonal. 1 ug/ml is usually best but many take 10 ug/ml to give a strong signal. But obviously monoclonals are to single epitopes and therefore different ones will have different success rates so it isn't shocking the ELISA monoclonal works and this one doesn't. I strongly suspect there are polyclonals to CEA available so I would consider going that route. Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Wednesday, March 30, 2011 1:58 AM To: Phillips, Thomas E.
Good day to all!
I have been trying to label the CEA factor in MCF-7 cells in culture by IF without success. I can measure CEA in whole cell extracts by ELISA using a kit so its presence is certain. Unfortunately the company does not sell the antibody used in the kit so I had to buy another primary antibody from another source (Mab H-8 from Santy Cruz). The secondary is a brand new antibody so it is not the culprit. My problem is that by IF I get no specific signal, just low background signal. It may be that the primary simply doesn't work but before I resign I would like to ask if someone may had an idea how to reveal the antigen better. I expect the antigen to be bound to the cell membrane.
Here is my method: - fixation for 15 min with 4% PFA in 0,1M phosphate buffer - permeabilization for 5 min (or not, I try both in parallel) with 0,01% triton x-100/PBS - blocking 30 min with 1% BSA/PBS - 1h30 at 37°C with anti-CEA Mab H-8 1/50 in 0,5% BSA/PBS - 1h30 at 37°C with GAM-alexa 488 1/100 in 0,5% BSA/PBS
Thank you in advance
Stephane
==============================Original Headers============================== 7, 34 -- From nizets2-at-yahoo.com Wed Mar 30 01:56:47 2011 7, 34 -- Received: from nm23.bullet.mail.ne1.yahoo.com (nm23.bullet.mail.ne1.yahoo.com [98.138.90.86]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2U6ulct015633 7, 34 -- for {microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 01:56:47 -0500 7, 34 -- Received: from [98.138.90.56] by nm23.bullet.mail.ne1.yahoo.com with NNFMP; 30 Mar 2011 06:56:47 -0000 7, 34 -- Received: from [98.138.87.6] by tm9.bullet.mail.ne1.yahoo.com with NNFMP; 30 Mar 2011 06:56:47 -0000 7, 34 -- Received: from [127.0.0.1] by omp1006.mail.ne1.yahoo.com with NNFMP; 30 Mar 2011 06:56:46 -0000 7, 34 -- X-Yahoo-Newman-Property: ymail-3 7, 34 -- X-Yahoo-Newman-Id: 985325.97781.bm-at-omp1006.mail.ne1.yahoo.com 7, 34 -- Received: (qmail 29678 invoked by uid 60001); 30 Mar 2011 06:56:46 -0000 7, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1301468206; bh=RFU8Nt2hi3pPZvRSp8/jNlna+o93raOIuSQFhmgndUc=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=mMCtGkwcZFX+lTAa1Nf2+VBf3ASWKQt8jPhxic2BWIfA71nvGlvLP8GeOWcIPU3wCdIehF3pK0IBENBsvnkWqZMCD1Bz5tLIIqKNN82fgBlT+tf+gNJ3+SVh8SUG2kz3Trt85JsFes3zv2hRlI2lOdYMKmNdsvUDv/nPilm8BeY= 7, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 34 -- s=s1024; d=yahoo.com; 7, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 34 -- b=Iar9AFrzOMq+x10ySWtRYHLkIZQ1IxpVIlSNUmZBwaxhxuao7UCuogo1a20ebdgxpD+s9S732OdjnbiB+kC5q6lkDlnTL+ifYdgbdlMTROFzjxdrA9TWi1uOSE+xkEIqLaFLL0B104Z6pos8TwUFo7xgiIU+PRTFfpHBZUQAe7g=; 7, 34 -- Message-ID: {47752.97466.qm-at-web110810.mail.gq1.yahoo.com} 7, 34 -- X-YMail-OSG: Cn4sOPMVM1k0h5I.IaEyNZV_SKpjlMcwmohctvBWtURIKSJ 7, 34 -- UJ7o6nKRQdy.x0Dz36Pn65Zw36HEyQl2_vPqRN15bNGjzfsQqct4YVhaeFpB 7, 34 -- Xn_GWeWIQitiDmpAqjvzDLj1S2ML6l7oFkTChEFRDm4fy_FIB8OgFdSKSsNo 7, 34 -- eVy.uCw6sijLQek9ekR18DorjKqArqmAAAN0KMyCLOZzCrFMwWby0HEbGa2D 7, 34 -- W02U37RnAZio3SXgACUfgPLSFaul62zyDepNSQxyvndBqyoAYdB58IDP.Q5b 7, 34 -- 8Lr6zvuB_wjrZb4fNg2nlUVgz9a_t1vcG766_67R2HKs0Umkh1ej.kv93exK 7, 34 -- _T0spTt0BBk7T7c477jWou3k3fwG9abjxPpRoQO.kYT5PuYPUJa.RnbLSXpq 7, 34 -- Cf73t4n6jXtk8wA-- 7, 34 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Tue, 29 Mar 2011 23:56:45 PDT 7, 34 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 7, 34 -- Date: Tue, 29 Mar 2011 23:56:45 -0700 (PDT) 7, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 7, 34 -- Subject: Advice for IF labeling 7, 34 -- To: microscopy-at-microscopy.com 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; charset=iso-8859-1 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2U6ulct015633 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 31 -- From prvs=0636a5068=PhillipsT-at-missouri.edu Wed Mar 30 08:00:14 2011 16, 31 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2UD0EMw023186 16, 31 -- for {microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 08:00:14 -0500 16, 31 -- X-IronPort-Anti-Spam-Filtered: true 16, 31 -- X-IronPort-Anti-Spam-Result: AvYAAPwnk03Raua2/2dsb2JhbACYEQ6NMXerRokqiGwCgxSCVASFP45I 16, 31 -- Received: from um-nhub02.um.umsystem.edu ([209.106.230.182]) 16, 31 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 30 Mar 2011 08:00:13 -0500 16, 31 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by 16, 31 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Wed, 30 Mar 2011 16, 31 -- 08:00:13 -0500 16, 31 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 31 -- To: "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} , 16, 31 -- "microscopy-at-microscopy.com" 16, 31 -- {microscopy-at-microscopy.com} 16, 31 -- Date: Wed, 30 Mar 2011 08:00:13 -0500 16, 31 -- Subject: RE: [Microscopy] Advice for IF labeling 16, 31 -- Thread-Topic: [Microscopy] Advice for IF labeling 16, 31 -- Thread-Index: Acvup8ohbtRMBwIQTtSh+SI03QRSLgAMPLPg 16, 31 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF267B51DE2F-at-UM-EMAIL05.um.umsystem.edu} 16, 31 -- References: {201103300657.p2U6vYG6016606-at-ns.microscopy.com} 16, 31 -- In-Reply-To: {201103300657.p2U6vYG6016606-at-ns.microscopy.com} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- acceptlanguage: en-US 16, 31 -- Content-Type: text/plain; charset="iso-8859-1" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2UD0EMw023186 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: University of Idaho
Title-Subject: [Filtered] Thanks for the help.
Message: I wanted to thank all the Listserv members for their help with my Oxford EDS system. Seems that the shutter is opening and closing but the system is having problems recognizing this. Once in the OPEN position, I just left it alone, checked the detector on a sample. Detector works fine. Good count rate and good calibration. Happy times in the TEM lab. I plan to leave the shutter open for now. As I told a student-it may die in a week, but it will die happy.
I need good antibodies (from different species, mouse and rabbit preferentially) against these two tags that would work for immunogold labeling on Tokuyasu cryosections. I think 6% formaldehyde, 4% formaldehyde/0.1% glutaraldehyde or 2% formaldehyde/0.2% glutaraldehyde would be the fixations I would try for the starters. Any recommendations?
Also, recommendations needed on suppliers of gold conjugates. For the last couple of years I was only using protein A from a single source that does not offer antibodies, so I do not have current information.
Thanks,
Michal
==============================Original Headers============================== 5, 23 -- From jarnikm-at-mail.nih.gov Wed Mar 30 08:27:05 2011 5, 23 -- Received: from nihrelayxway6.hub.nih.gov (nihrelayxway6.hub.nih.gov [128.231.90.100]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2UDR5lJ023325 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 08:27:05 -0500 5, 23 -- X-IronPortListener: NIH_Relay 5, 23 -- X-SBRS: None 5, 23 -- X-IronPort-AV: E=Sophos;i="4.63,268,1299474000"; 5, 23 -- d="scan'208";a="25543087" 5, 23 -- Received: from mailfwd.nih.gov ([128.231.90.107]) 5, 23 -- by nihrelayxway6.hub.nih.gov with ESMTP; 30 Mar 2011 09:27:05 -0400 5, 23 -- Received: from [156.40.161.43] (156.40.161.43) by mailfwd.nih.gov 5, 23 -- (128.231.90.107) with Microsoft SMTP Server id 14.1.255.0; Wed, 30 Mar 2011 5, 23 -- 09:26:51 -0400 5, 23 -- From: Michal Jarnik {jarnikm-at-mail.nih.gov} 5, 23 -- Content-Type: text/plain; charset="us-ascii" 5, 23 -- Subject: Anti-HA and anti-myc tag antibodies for immunogold 5, 23 -- Date: Wed, 30 Mar 2011 09:27:04 -0400 5, 23 -- Message-ID: {576E1964-6492-4BD2-B49C-AFBE15599C31-at-mail.nih.gov} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- MIME-Version: 1.0 (Apple Message framework v1082) 5, 23 -- X-Mailer: Apple Mail (2.1082) 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2UDR5lJ023325 ==============================End of - Headers==============================
Lincoln Electric has a position open for a metallographer. The microscopy lab has the typical equipment needed for metallography in a production setting: SEM, stereomicroscope, reflected light, microhardness, grinding and polishing wheels. The add talks about engineers, but that because Lincoln Electric ( the welding company) always ask for engineers but we need a metallographer. Here's the truncated add:
The Engineer - Mechanical Lab has the following responsibilities: Work in the Mechanical and/or Microscopy Laboratory analyzing and characterizing chemicals, metals, and weld deposits using routine and non-routine procedures of substantial variety and complexity.
Requirements Minimum four-year engineering degree required. Metallurgical, Materials, or Mechanical Engineering degree is required. Specialty in welding is desirable. 3 – 5 years of professional experience preferred. Experience with mechanical testing equipment and specifications is required. Familiarity with NDT techniques and microscopy is desirable. Knowledge of basic statistical methods is required. Knowledge of Six Sigma methodologies is desirable.
Contact: Yonatan Necoechea Manager, Specials & Testing Services The Lincoln Electric Company Office: 216.383.5439 22801 St. Clair Ave. | Cleveland, OH | 44117 -- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 8, 17 -- From frank_karl-at-lincolnelectric.com Wed Mar 30 09:41:24 2011 8, 17 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 8, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2UEfOTo008438 8, 17 -- for {microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 09:41:24 -0500 8, 17 -- Subject: position to fill 8, 17 -- To: Microscopy-at-microscopy.com 8, 17 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 8, 17 -- Message-ID: {OF853FC11E.65BC41BF-ON85257863.004FAD5C-85257863.0050AF38-at-lincolnelectric.com} 8, 17 -- From: Frank_Karl-at-lincolnelectric.com 8, 17 -- Date: Wed, 30 Mar 2011 10:41:18 -0400 8, 17 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2 HF23|September 01, 2010) at 8, 17 -- 03/30/2011 10:41:19 AM 8, 17 -- MIME-Version: 1.0 8, 17 -- Content-Type: text/plain; 8, 17 -- charset="UTF-8" 8, 17 -- Content-Transfer-Encoding: 8bit 8, 17 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p2UEfOTo008438 ==============================End of - Headers==============================
Hi Micheal, We use standard 2% PFA + 0,2% GA or only 2% PFA. Antibodies for myc-tag are 9E10 (M) or NKI-C3 (available as moab and poab) both work well on material fixed with the first fixative. For HA-tag we use 12CA5 (M) but this doesn't always work that good. All the mentioned ab's are commercially available.
Groeten, Hans. Netherlands Cancer Institute. Amsterdam.
-----Original Message----- X-from: jarnikm-at-mail.nih.gov [mailto:jarnikm-at-mail.nih.gov] Sent: woensdag 30 maart 2011 15:32 To: Hans Janssen
Dear list,
I need good antibodies (from different species, mouse and rabbit preferentially) against these two tags that would work for immunogold labeling on Tokuyasu cryosections. I think 6% formaldehyde, 4% formaldehyde/0.1% glutaraldehyde or 2% formaldehyde/0.2% glutaraldehyde would be the fixations I would try for the starters. Any recommendations?
Also, recommendations needed on suppliers of gold conjugates. For the last couple of years I was only using protein A from a single source that does not offer antibodies, so I do not have current information.
Thanks,
Michal
==============================Original Headers============================== 5, 23 -- From jarnikm-at-mail.nih.gov Wed Mar 30 08:27:05 2011 5, 23 -- Received: from nihrelayxway6.hub.nih.gov (nihrelayxway6.hub.nih.gov [128.231.90.100]) 5, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2UDR5lJ023325 5, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 08:27:05 -0500 5, 23 -- X-IronPortListener: NIH_Relay 5, 23 -- X-SBRS: None 5, 23 -- X-IronPort-AV: E=Sophos;i="4.63,268,1299474000"; 5, 23 -- d="scan'208";a="25543087" 5, 23 -- Received: from mailfwd.nih.gov ([128.231.90.107]) 5, 23 -- by nihrelayxway6.hub.nih.gov with ESMTP; 30 Mar 2011 09:27:05 -0400 5, 23 -- Received: from [156.40.161.43] (156.40.161.43) by mailfwd.nih.gov 5, 23 -- (128.231.90.107) with Microsoft SMTP Server id 14.1.255.0; Wed, 30 Mar 2011 5, 23 -- 09:26:51 -0400 5, 23 -- From: Michal Jarnik {jarnikm-at-mail.nih.gov} 5, 23 -- Content-Type: text/plain; charset="us-ascii" 5, 23 -- Subject: Anti-HA and anti-myc tag antibodies for immunogold 5, 23 -- Date: Wed, 30 Mar 2011 09:27:04 -0400 5, 23 -- Message-ID: {576E1964-6492-4BD2-B49C-AFBE15599C31-at-mail.nih.gov} 5, 23 -- To: {Microscopy-at-microscopy.com} 5, 23 -- MIME-Version: 1.0 (Apple Message framework v1082) 5, 23 -- X-Mailer: Apple Mail (2.1082) 5, 23 -- Content-Transfer-Encoding: 8bit 5, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2UDR5lJ023325 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 25 -- From j.janssen-at-nki.nl Wed Mar 30 10:02:24 2011 13, 25 -- Received: from edge.nki.nl (edge-1.nki.nl [194.171.7.92]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2UF2OcZ026372 13, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Mar 2011 10:02:24 -0500 13, 25 -- Received: from caht-1.nki.nl (194.171.7.85) by edge-1.nki.nl (194.171.7.92) 13, 25 -- with Microsoft SMTP Server (TLS) id 8.2.254.0; Wed, 30 Mar 2011 17:02:22 13, 25 -- +0200 13, 25 -- Received: from mstr-1.nki.nl ([172.31.4.101]) by caht-1.nki.nl 13, 25 -- ([172.31.4.103]) with mapi; Wed, 30 Mar 2011 17:02:23 +0200 13, 25 -- From: {j.janssen-at-nki.nl} 13, 25 -- To: {Microscopy-at-microscopy.com} 13, 25 -- Date: Wed, 30 Mar 2011 17:02:22 +0200 13, 25 -- Subject: FW: [Microscopy] Anti-HA and anti-myc tag antibodies for immunogold 13, 25 -- Thread-Topic: [Microscopy] Anti-HA and anti-myc tag antibodies for immunogold 13, 25 -- Thread-Index: Acvu3swBlGLZOcqbQJu1R8cvfgQxLAAC5hDQ 13, 25 -- Message-ID: {F7BE8A0314FCF948BD9CFA10E54AEABD2D0B3D7B-at-mstr-1.nki.nl} 13, 25 -- Accept-Language: nl-NL, en-US 13, 25 -- Content-Language: en-US 13, 25 -- X-MS-Has-Attach: 13, 25 -- X-MS-TNEF-Correlator: 13, 25 -- acceptlanguage: nl-NL, en-US 13, 25 -- Content-Type: text/plain; charset="us-ascii" 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-Transfer-Encoding: 8bit 13, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2UF2OcZ026372 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Loughborough University
Title-Subject: [Filtered] EDS unexpected Si peak Message: Dear All,
I use a 2000FX TEM at 200kV equipped with an Oxford Inca EDS, ultra-thin window Si-Li detector. Sometimes I get Si K line from a sample which should have no Si at all.
Can anybody please explain where the Si peak comes from?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zasad008-at-umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Does anyone support the RMC/JEOL RFD 9010 freeze-fracture equipment? I am trying to find someone to help set up and trouble shoot an instrument recently moved from UC Santa Barbara. There are a few things that need repairing or replacing, but I am at a loss as to whom to contact.
Can you recommend a new embedding system for TEM sample preparation or do you have any suggestions on how to stop my drift issue?
I have been using a lower viscosity system for years. LR White (Medium or Hard) has been part of my TEM sample preparation for many different microscopes (TEM - JEOL 100cx, 2010LB6, 2010F, 2500F, etc). The only issue I have ever seen was when the carbon coating was too thin, Pop! I just started using a JEOL 2100-Cryo and the sections are drifting horrifically (LR White Medium).
Background, etc.. I section my samples because I need views down the major zone axis. The samples I'm looking at are beam sensitive. Thus, I setup the JEOL 2100-Cryo microscope in a lower dose condition. I also tried using the MDS. The viewing time before beam destruction of the structure appears to be about the same as if I were viewing the sample on my 2010LB6. I also looked at a Pt/Ir Cal. sample and found no drift issues at 1,000,000x.
I have tried the following with my LR White sections before viewing them on the 2100-Cryo (magnification 250KX-500KX): - Normal carbon coating - Pop! - Heaver carbon coating one side - Drift - Same heavy coating but on both sides - Drift - Heavy carbon coating on one side using a cryo-sample holder - Drift was greatly minimized but at -170C there was too much ice on the sample for HRTEM - Sections were placed on an ultra thin carbon film and then I added a heavy carbon coating - Drift
Possible other options to try: - Change the embedding system (to what?) - Insert the 100um or 75um OL aperture - Try cryo at an elevated temperature -80 or -120C (I would like to run samples at room temp.) - It's time to retire (still too early for this)
Thanks, Tom
==============================Original Headers============================== 7, 33 -- From lets_bike-at-yahoo.com Thu Mar 31 09:04:48 2011 7, 33 -- Received: from nm11.bullet.mail.ne1.yahoo.com (nm11.bullet.mail.ne1.yahoo.com [98.138.90.74]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2VE4leb031932 7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 09:04:47 -0500 7, 33 -- Received: from [98.138.90.53] by nm11.bullet.mail.ne1.yahoo.com with NNFMP; 31 Mar 2011 14:04:47 -0000 7, 33 -- Received: from [98.138.89.248] by tm6.bullet.mail.ne1.yahoo.com with NNFMP; 31 Mar 2011 14:04:46 -0000 7, 33 -- Received: from [127.0.0.1] by omp1040.mail.ne1.yahoo.com with NNFMP; 31 Mar 2011 14:04:46 -0000 7, 33 -- X-Yahoo-Newman-Property: ymail-3 7, 33 -- X-Yahoo-Newman-Id: 924672.76527.bm-at-omp1040.mail.ne1.yahoo.com 7, 33 -- Received: (qmail 11272 invoked by uid 60001); 31 Mar 2011 14:04:46 -0000 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1301580286; bh=iYAeC2m4XQl6moPpSzAgq3cwW7czUejeZ8w9VY2cZIA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=T5hhYAId20rUps/Lf6bWDfwMhaV34t5+L/H2ToyAiVMUe0cdpo8a7Tx7Mny+EBtRSAGHI67nhVu7TNeh1nU60jFTnac+bJ5qPaWpL/JbLlShBSB16RHDi+V0ZAp6NjSKJztgUVRUYJ1XCfhczfdOq/2KUgwqPQTzldR1/N3xXyo= 7, 33 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 33 -- s=s1024; d=yahoo.com; 7, 33 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 7, 33 -- b=17Y+X6RD3AurBpDnuHzqOlxSgq5/aur5Jhqc+mGBJBOxeHbNEWap0Camn4pw7w6FkLZtxOomEPKPIkipmx1XTE69r7/79EVgC8L9q70jlbRUMw2Heztp1zqjLeBZxnTztJzpLeP+c5fOvlsWdE9U1ooWpcgfb22fjpJjTee8VLo=; 7, 33 -- Message-ID: {801685.84625.qm-at-web121816.mail.ne1.yahoo.com} 7, 33 -- X-YMail-OSG: j5dZnYYVM1kNbjcFtWpz7e7z6I2Ff.HkWE_XdBAoYcIlDit 7, 33 -- T2OeNlLj1P2ZG3OMBk3d2phdYNrYREwa.SXLYN_02F6jzCMCMKBvOvIUtDcd 7, 33 -- WXUoTwKJBJNCHQqv5I6X0ZIe6Qsi4fLPUMczJwxE13WgYNpwOcd7cCqb7sMh 7, 33 -- Sc0QhlyTUfpG5.dLUlfSTZXiUXAESXg3zYsuwpEj7ejzY4VnDdJesPPXC_kG 7, 33 -- _ZyS108Ghkomrdwl1SLKhnhI_RUYA05QYUTCt2T6ea.FJKXTdPhOJOXCXWO. 7, 33 -- XyC15NDvS6gZ18e68JR5YC8mPNva4XDxnPjEJ4spv45..H7YlxthYsKxC0V3 7, 33 -- xK6Aq_YaQYsqe.T4C3gDVQ4VMP2NbRedHZCTmvHT41jcpbtF3kyl5Dw-- 7, 33 -- Received: from [144.5.140.24] by web121816.mail.ne1.yahoo.com via HTTP; Thu, 31 Mar 2011 07:04:46 PDT 7, 33 -- X-Mailer: YahooMailClassic/12.0.2 YahooMailWebService/0.8.109.295617 7, 33 -- Date: Thu, 31 Mar 2011 07:04:46 -0700 (PDT) 7, 33 -- From: Tom {lets_bike-at-yahoo.com} 7, 33 -- Subject: TEM - looking for a more stable embedding system 7, 33 -- To: Microscopy-at-microscopy.com 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Type: text/plain; charset=iso-8859-1 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p2VE4leb031932 ==============================End of - Headers==============================
I can think of two reasons for seeing the Si K peak.
First is the silicon internal flourescence peak, that is, the SiLi detector itself emits Si K x-rays from the "dead layer," which are then detected by the "active layer" of the detector. I have experienced this in some detectors more than in others.
Second may be contamination from Si-based vacuum grease, transferred from o-ring to loadlock wall to sample holder tip, during sample holder insertion and retraction. Of course, it could also be from sample contamination: We had an associate that habitually secured the TEM sample to the ion-milling stub with Si-grease. This got transferred to the TEM holder and appeared in all EDX spectra on that instrument. A thorough cleaning of the TEM sample holder eliminated the Si K peak due to this problem.
Good luck Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Loughborough University
Title-Subject: [Filtered] EDS unexpected Si peak Message: Dear All,
I use a 2000FX TEM at 200kV equipped with an Oxford Inca EDS, ultra-thin window Si-Li detector. Sometimes I get Si K line from a sample which should have no Si at all.
Can anybody please explain where the Si peak comes from?
Zhou
==============================Original Headers============================== 15, 22 -- From raristau-at-ims.uconn.edu Thu Mar 31 09:20:38 2011 15, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 15, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2VEKcVx015913 15, 22 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 09:20:38 -0500 15, 22 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu [137.99.20.234]) 15, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 701E22CF27; 15, 22 -- Thu, 31 Mar 2011 10:20:38 -0400 (EDT) 15, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 15, 22 -- Date: Thu, 31 Mar 2011 10:20:38 -0400 15, 22 -- Subject: re:[Microscopy] viaWWW:EDS unexpected Si peak 15, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 15, 22 -- To: {z.zhou-at-lboro.ac.uk} , 15, 22 -- "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 15, 22 -- Message-ID: {C9BA05F6.26F0%raristau-at-ims.uconn.edu} 15, 22 -- Thread-Topic: [Microscopy] viaWWW:EDS unexpected Si peak 15, 22 -- Thread-Index: Acvvrs6dDOHNCluiEeC3uQAbY55CVA== 15, 22 -- Mime-version: 1.0 15, 22 -- Content-type: text/plain; 15, 22 -- charset="US-ASCII" 15, 22 -- Content-transfer-encoding: 7bit 15, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 15, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Dear Roger & Zhou, I ran into one additional source of Si. A specimen had been placed on a glass slide and subjected to evaporation, then embedded and sectioned. Sure enough, there was a prominent Si peak. Yours, Bill
X-from: raristau-at-ims.uconn.edu To: William.F.Tivol-at-aero.org
I can think of two reasons for seeing the Si K peak.
First is the silicon internal flourescence peak, that is, the SiLi detector itself emits Si K x-rays from the "dead layer," which are then detected by the "active layer" of the detector. I have experienced this in some detectors more than in others.
Second may be contamination from Si-based vacuum grease, transferred from o-ring to loadlock wall to sample holder tip, during sample holder insertion and retraction. Of course, it could also be from sample contamination: We had an associate that habitually secured the TEM sample to the ion-milling stub with Si-grease. This got transferred to the TEM holder and appeared in all EDX spectra on that instrument. A thorough cleaning of the TEM sample holder eliminated the Si K peak due to this problem.
Good luck Roger
Roger A. Ristau, PhD
Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Loughborough University
Title-Subject: [Filtered] EDS unexpected Si peak Message: Dear All,
I use a 2000FX TEM at 200kV equipped with an Oxford Inca EDS, ultra-thin window Si-Li detector. Sometimes I get Si K line from a sample which should have no Si at all.
Can anybody please explain where the Si peak comes from?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2VHx3pP020413 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 31 Mar 2011 12:59:03 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p2VHx3G6020411; Thu, 31 Mar 2011 12:59:03 -0500
Hello All,
Some types of grid storage boxes may also be sources of silicone contamination. If your samples are supported on grids, collect a spectrum from an area of the grid film without sample present for comparison, or put an unused grid into the scope and analyze a few areas to see whether anything other than carbon is present.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
-----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Thursday, March 31, 2011 11:34 AM To: Elaine F. Schumacher
Dear Roger & Zhou, I ran into one additional source of Si. A specimen had been placed on a glass slide and subjected to evaporation, then embedded and sectioned. Sure enough, there was a prominent Si peak. Yours, Bill
X-from: raristau-at-ims.uconn.edu To: William.F.Tivol-at-aero.org
I can think of two reasons for seeing the Si K peak.
First is the silicon internal flourescence peak, that is, the SiLi detector itself emits Si K x-rays from the "dead layer," which are then detected by the "active layer" of the detector. I have experienced this in some detectors more than in others.
Second may be contamination from Si-based vacuum grease, transferred from o-ring to loadlock wall to sample holder tip, during sample holder insertion and retraction. Of course, it could also be from sample contamination: We had an associate that habitually secured the TEM sample to the ion-milling stub with Si-grease. This got transferred to the TEM holder and appeared in all EDX spectra on that instrument. A thorough cleaning of the TEM sample holder eliminated the Si K peak due to this problem.
Good luck Roger
Roger A. Ristau, PhD
Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Loughborough University
Title-Subject: [Filtered] EDS unexpected Si peak Message: Dear All,
I use a 2000FX TEM at 200kV equipped with an Oxford Inca EDS, ultra-thin window Si-Li detector. Sometimes I get Si K line from a sample which should have no Si at all.
Can anybody please explain where the Si peak comes from?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2VJHenO005686 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 31 Mar 2011 14:17:40 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p2VJHev5005684; Thu, 31 Mar 2011 14:17:40 -0500
Most modern detectors have much better resolution that in the past, but you may want to see if you have an overlap with a heavier element. If memory serves me Ta or W had m lines close to the Si k line. If your Si line is very high then this will be less likely.
Your settings on the Oxford may be such that if you are using auto ID the software is identifying it as Si to be sure you look for it.
Tommy D NSWC Crane
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, March 30, 2011 8:49 PM To: gtuser-at-comcast.net
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-lboro.ac.uk Name: Zhou
Organization: Loughborough University
Title-Subject: [Filtered] EDS unexpected Si peak Message: Dear All,
I use a 2000FX TEM at 200kV equipped with an Oxford Inca EDS, ultra-thin window Si-Li detector. Sometimes I get Si K line from a sample which should have no Si at all.
Can anybody please explain where the Si peak comes from?
I am looking for some tips to collect a diffration pattern of nanoparticles using a TEM JEM2100 with a high resolution camera Gatan Orius SC600, without damaging the sensitive CCD. I appreciate any help.
Thanks,
Marcela.  ------ Marcela Redigolo Shared Research Facilities marcela.redigolo-at-mail.wvu.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both luo-at-mic.tamu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: luo-at-mic.tamu.edu Name: Zhiping Luo
Organization: Texas A&M University
Title-Subject: [Filtered] RE: EDS unexpected Si peak
Message: Hi all,
This is a good subject for discussion. A way to identify the Si source is to collect the spectra at difference spots. If the Si is seen on the particle (or phase A), but not on the support film background (or phase B), then we can say the Si is from the sample itself.
In the case Si is seen everywhere even on the hole without specimen, then it is from the TEM system - service is needed!
Considering the spurious signals in the TEM, the different spots tested should be far away enough.
Occasionally I receive the complaints about the Si that is not supposed to have, mostly from the chemistry users. I prove to them that it is the sample contaminated with Si, rather than the TEM. If the TEM is well aligned, the EDS is quite reliable.
Sincerely,
Zhiping Luo
Microscopy and Imaging Center Texas A&M University
Hello All, Some types of grid storage boxes may also be sources of silicone contamination. If your samples are supported on grids, collect a spectrum from an area of the grid film without sample present for comparison, or put an unused grid into the scope and analyze a few areas to see whether anything other than carbon is present. Regards, Elaine ********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. ********************************************************************* -----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Thursday, March 31, 2011 11:34 AM To: Elaine F. Schumacher Dear Roger & Zhou, I ran into one additional source of Si. A specimen had been placed on a glass slide and subjected to evaporation, then embedded and sectioned. Sure enough, there was a prominent Si peak. Yours, Bill X-from: raristau-at-ims.uconn.edu To: William.F.Tivol-at-aero.org I can think of two reasons for seeing the Si K peak. First is the silicon internal flourescence peak, that is, the SiLi detector itself emits Si K x-rays from the "dead layer," which are then detected by the "active layer" of the detector. I have experienced this in some detectors more than in others. Second may be contamination from Si-based vacuum grease, transferred from o-ring to loadlock wall to sample holder tip, during sample holder insertion and retraction. Of course, it could also be from sample contamination: We had an associate that habitually secured the TEM sample to the ion-milling stub with Si-grease. This got transferred to the TEM holder and appeared in all EDX spectra on that instrument. A thorough cleaning of the TEM sample holder eliminated the Si K peak due to this problem. Good luck Roger Roger A. Ristau, PhD Email: z.zhou-at-lboro.ac.uk Name: Zhou Organization: Loughborough University Title-Subject: [Filtered] EDS unexpected Si peak Message: Dear All, I use a 2000FX TEM at 200kV equipped with an Oxford Inca EDS, ultra-thin window Si-Li detector. Sometimes I get Si K line from a sample which should have no Si at all. Can anybody please explain where the Si peak comes from?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3120RWU028462 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 31 Mar 2011 21:00:27 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3120Rl9028460; Thu, 31 Mar 2011 21:00:27 -0500
Dear Bradford Ross:
Thanks for your reply! This is the process I am used to when working with other TEMs.
This TEM we have has two cameras. The upper one used for great part of the measurements, and the SC600 that was installed for high resolution imaging for certain interface analyses. I was wondering if, different from the usual process, is there any other settings to use with this camera without causing damage.
I am new to this microscope and apparently in the past someone caused a damage to the camera, because of the diffraction pattern. It's my belief that something was done wrong and the usual process would work just fine. But I decided to check just to be sure I'm not missing anything.
Thanks for the help, I appreciate.
Marcela.
--- Dr. Marcela Redigolo TEM Specialist Shared Research Facilities (214) 766-2904
Your TEM needs to have a beam stop above the camera to capture diffraction patterns. Typically this is inserted and adjusted with a small knob on the side of the column just above the viewing chamber.
Normally you would get the diffraction pattern you want to record on the fluorescent viewing screen, then insert the beam stop to a point that covers the bright central incident spot of the diffraction pattern, and then reduce the brightness of the pattern as much as possible with the brightness/intensity/C2 knob to reduce the possibility of damage to the CCD. After this is done it should be ok to lift the fluorescent screen and acquire an image with the camera. Once you have acquired the image, immediately lower the fluorescent screen again to protect the CCD.
If you need to change camera lengths, do not do this while the pattern is being viewed/recorded on the camera because the pattern may change position and the central spot could move away from the beam stop and damage the CCD.
Hope this helps,
Bradford Ross
Microscopy Technician BioImaging Facility University of British Columbia 6270 University Blvd. Vancouver, B.C. Canada V6T 1Z4
phone 604-822-6996
________________________________________ X-from: marcela.redigolo-at-mail.wvu.edu [marcela.redigolo-at-mail.wvu.edu] Sent: Thursday, March 31, 2011 12:28 PM To: bnross-at-interchange.ubc.ca
Dear all,
I am looking for some tips to collect a diffration pattern of nanoparticles using a TEM JEM2100 with a high resolution camera Gatan Orius SC600, without damaging the sensitive CCD. I appreciate any help.
Thanks,
Marcela.
==============================Original Headers============================== 43, 28 -- From Marcela.Redigolo-at-mail.wvu.edu Thu Mar 31 21:00:27 2011 43, 28 -- Received: from mx6.wvu.edu (mx6.wvu.edu [157.182.203.35]) 43, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3120RlP028457 43, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 21:00:27 -0500 43, 28 -- Received: from mx6.wvu.edu (localhost [127.0.0.1]) 43, 28 -- by localhost (Postfix) with SMTP id 24B9630E9 43, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 22:00:27 -0400 (EDT) 43, 28 -- Received: from WVUGW01.wvu.edu (unknown [157.182.47.70]) 43, 28 -- (using TLSv1 with cipher ADH-AES256-SHA (256/256 bits)) 43, 28 -- (No client certificate requested) 43, 28 -- by mx6.wvu.edu (Postfix) with ESMTPS id 531F630E3 43, 28 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 22:00:26 -0400 (EDT) 43, 28 -- Received: from WVUDOM1-MTA by WVUGW01.wvu.edu 43, 28 -- with Novell_GroupWise; Thu, 31 Mar 2011 22:00:26 -0400 43, 28 -- Message-Id: {4D94F9780200008C0009B941-at-WVUGW01.wvu.edu} 43, 28 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 43, 28 -- Date: Thu, 31 Mar 2011 22:00:23 -0400 43, 28 -- From: "Marcela Redigolo" {Marcela.Redigolo-at-mail.wvu.edu} 43, 28 -- To: {microscopy-at-microscopy.com} 43, 28 -- Subject: RE: [Microscopy] Diffraction w/ Gatan SC600 TEM J2100 43, 28 -- Mime-Version: 1.0 43, 28 -- Content-Type: text/plain; charset=US-ASCII 43, 28 -- Content-Disposition: inline 43, 28 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.4.1.15118 43, 28 -- X-WVU-PMX-SCORE: Gauge=X, Probability=10%, Report=' 43, 28 -- TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, BODY_SIZE_3000_3999 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CD 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' 43, 28 -- Content-Transfer-Encoding: 8bit 43, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3120RlP028457 ==============================End of - Headers==============================
Hi Marcela, If you have an Orius camera you are well set up for diffraction. They are almost bullet proof and have anti-blooming, so spots stay sharp. If you are sensible and careful there is no need for a beam stop. Use small condenser apertures and parallel illumination with a selected area aperture and well-focused SADP, you can have the pattern on the screen for minutes with no obvious damage. Our camera was damaged by a user who had a large condenser aperture, no SA aperture, and an unfocused diffraction pattern giving a very high intensity caustic - i.e. Did -----Original Message----- X-from: marcela.redigolo-at-mail.wvu.edu
Dear all,
I am looking for some tips to collect a diffration pattern of nanoparticles using a TEM JEM2100 with a high resolution camera Gatan Orius SC600, without damaging the sensitive CCD. I appreciate any help.
Thanks,
Marcela.  ------ Marcela Redigolo Shared Research Facilities marcela.redigolo-at-mail.wvu.edu
I never did cryo but from what you wrote it seems that you already tried the most important things. Inserting the objective aperture will certainly help, but it brings a strong background signal in EDX. The advantage is that it is very easy to try. You may definitely try another resin, look in the manufacturer sites for the most usual of them. You may also try crazy things, sometimes it works and desperate cases are the best occasions to try them. In your case you may want to add some conductive powder in the embedding medium for example. There should be a critical concentration which is transparent enough to the electron while giving enough conductivity to avoid drift. Once I had a big problem of drift and I coated with a fine silver layer and it worked wonderfully. It is probably not suitable for high magnifications but at low magnification the structure of silver deposit was not visible. Finally, retirement is always the best solution to solve problems at work. So sad it has such a financial drawback!
Regards, Stephane
----- Original Message ---- X-from: "lets_bike-at-yahoo.com" {lets_bike-at-yahoo.com} To: nizets2-at-yahoo.com Sent: Thu, March 31, 2011 4:09:55 PM
Can you recommend a new embedding system for TEM sample preparation or do you have any suggestions on how to stop my drift issue?
I have been using a lower viscosity system for years. LR White (Medium or Hard) has been part of my TEM sample preparation for many different microscopes (TEM - JEOL 100cx, 2010LB6, 2010F, 2500F, etc). The only issue I have ever seen was when the carbon coating was too thin, Pop! I just started using a JEOL 2100-Cryo and the sections are drifting horrifically (LR White Medium).
Background, etc.. I section my samples because I need views down the major zone axis. The samples I'm looking at are beam sensitive. Thus, I setup the JEOL 2100-Cryo microscope in a lower dose condition. I also tried using the MDS. The viewing time before beam destruction of the structure appears to be about the same as if I were viewing the sample on my 2010LB6. I also looked at a Pt/Ir Cal. sample and found no drift issues at 1,000,000x.
I have tried the following with my LR White sections before viewing them on the 2100-Cryo (magnification 250KX-500KX): - Normal carbon coating - Pop! - Heaver carbon coating one side - Drift - Same heavy coating but on both sides - Drift - Heavy carbon coating on one side using a cryo-sample holder - Drift was greatly minimized but at -170C there was too much ice on the sample for HRTEM
- Sections were placed on an ultra thin carbon film and then I added a heavy carbon coating - Drift
Possible other options to try: - Change the embedding system (to what?) - Insert the 100um or 75um OL aperture - Try cryo at an elevated temperature -80 or -120C (I would like to run samples at room temp.) - It's time to retire (still too early for this)
Thanks, Tom
==============================Original Headers============================== 7, 33 -- From lets_bike-at-yahoo.com Thu Mar 31 09:04:48 2011 7, 33 -- Received: from nm11.bullet.mail.ne1.yahoo.com (nm11.bullet.mail.ne1.yahoo.com [98.138.90.74]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p2VE4leb031932 7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 09:04:47 -0500 7, 33 -- Received: from [98.138.90.53] by nm11.bullet.mail.ne1.yahoo.com with NNFMP; 31 Mar 2011 14:04:47 -0000 7, 33 -- Received: from [98.138.89.248] by tm6.bullet.mail.ne1.yahoo.com with NNFMP; 31 Mar 2011 14:04:46 -0000 7, 33 -- Received: from [127.0.0.1] by omp1040.mail.ne1.yahoo.com with NNFMP; 31 Mar 2011 14:04:46 -0000 7, 33 -- X-Yahoo-Newman-Property: ymail-3 7, 33 -- X-Yahoo-Newman-Id: 924672.76527.bm-at-omp1040.mail.ne1.yahoo.com 7, 33 -- Received: (qmail 11272 invoked by uid 60001); 31 Mar 2011 14:04:46 -0000 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1301580286; bh=iYAeC2m4XQl6moPpSzAgq3cwW7czUejeZ8w9VY2cZIA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=T5hhYAId20rUps/Lf6bWDfwMhaV34t5+L/H2ToyAiVMUe0cdpo8a7Tx7Mny+EBtRSAGHI67nhVu7TNeh1nU60jFTnac+bJ5qPaWpL/JbLlShBSB16RHDi+V0ZAp6NjSKJztgUVRUYJ1XCfhczfdOq/2KUgwqPQTzldR1/N3xXyo= = 7, 33 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 7, 33 -- s=s1024; d=yahoo.com; 7, 33 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding;
That user didn't really know what they were doing; before it would only been a sheet of film but getting a digital camera fixed is expensive. On the other hand they can probably send an email, using only their thumbs, better than I can...
Good luck
Richard
On 31/03/2011 20:27, marcela.redigolo-at-mail.wvu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear all, } } I am looking for some tips to collect a diffration pattern of nanoparticles } using a TEM JEM2100 with a high resolution camera Gatan Orius SC600, without } damaging the sensitive CCD. I appreciate any help. } } Thanks, } } Marcela. }  } ------ } Marcela Redigolo } Shared Research Facilities } marcela.redigolo-at-mail.wvu.edu } } } } ==============================Original Headers============================== } 4, 28 -- From marcela.redigolo-at-mail.wvu.edu Thu Mar 31 14:23:05 2011 } 4, 28 -- Received: from mxs2.wvu.edu (mxs2.wvu.edu [157.182.140.114]) } 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p2VJN50v014121 } 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 14:23:05 -0500 } 4, 28 -- Received: from mxs2.wvu.edu (localhost [127.0.0.1]) } 4, 28 -- by localhost (Postfix) with SMTP id C914B187E2 } 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 15:23:04 -0400 (EDT) } 4, 28 -- Received: from CRRHLSGHJLRM1 (smtp-float.wvu.edu [157.182.47.70]) } 4, 28 -- (using TLSv1 with cipher RC4-MD5 (128/128 bits)) } 4, 28 -- (No client certificate requested) } 4, 28 -- by mxs2.wvu.edu (Postfix) with ESMTPS id 7E277187DF } 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 31 Mar 2011 15:23:04 -0400 (EDT) } 4, 28 -- MIME-Version: 1.0 } 4, 28 -- Message-Id: {4D94D4BF.000017.01836-at-CRRHLSGHJLRM1} } 4, 28 -- Date: Thu, 31 Mar 2011 15:23:43 -0400 (Eastern Daylight Time) } 4, 28 -- Content-Type: Text/Plain; } 4, 28 -- charset="utf-8" } 4, 28 -- X-Mailer: IncrediMail (6284944) } 4, 28 -- From: "M. Redigolo" {marcela.redigolo-at-mail.wvu.edu} } 4, 28 -- X-FID: PLAINTXT-NONE-0000-0000-000000000000 } 4, 28 -- Content-Transfer-Encoding: 7bit } 4, 28 -- X-Priority: 3 } 4, 28 -- To: {Microscopy-at-microscopy.com} } 4, 28 -- Subject: Diffraction w/ Gatan SC600 TEM J2100 } 4, 28 -- Reply-To: "M. Redigolo" {marcela.redigolo-at-mail.wvu.edu} } 4, 28 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.2.16.155719 } 4, 28 -- X-WVU-PMX-SCORE: Gauge=IIIIIIII, Probability=8%, Report=' } 4, 28 -- BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_300_399 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, INVALID_MSGID_NO_FQDN 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HAS_X_MAILER 0, __HAS_X_PRIORITY 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NO_PATH 0, __URI_NO_WWW 0, __URI_NS , __USER_AGENT_INCREDIMAIL 0' } ==============================End of - Headers==============================
Occasionally we have requests for diffraction patterns on the slow scan CCD camera on our FEGTEMs. Two tips I can offer are these:
1. Start with high spot sizes (high excitations of the first condenser lens). This will reduce the beam current, but improves the sharpness of the diffraction spots (all other things being equal).
2. Keep the exposure times reasonably short ( {0.1 seconds) in the first instance. Check the maximum intensity against the dynamic range of the camera (your 14-bit camera may have a realistic maximum intensity of 14000 counts per pixel). Find the exposure time that will give get about 10000 counts maximum and then use multiple exposures to boost the total acquisition time. In Digital Micrograph this will be via "Camera} Acquisition Parameters} Acquire" scroll down menus. On the "GIF CCD Setup" tab you will see a panel labelled "Processing" and "Frame Sum(# frames)". This is usually set to "1" for a single shot image. Set this to 10 or more for a high signal diffraction pattern.
I've used this method a number of times to get reasonably sharp diffraction patterns with good success.
Jon
On Mar 31 2011, marcela.redigolo-at-mail.wvu.edu wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } Dear all, } } I am looking for some tips to collect a diffration pattern of } nanoparticles using a TEM JEM2100 with a high resolution camera Gatan } Orius SC600, without damaging the sensitive CCD. I appreciate any help. } } Thanks, } } Marcela.
==============================Original Headers============================== 8, 28 -- From jsb43-at-hermes.cam.ac.uk Fri Apr 1 07:08:43 2011 8, 28 -- Received: from ppsw-41.csi.cam.ac.uk (ppsw-41.csi.cam.ac.uk [131.111.8.141]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p31C8hiC012228 8, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:08:43 -0500 8, 28 -- X-Cam-AntiVirus: no malware found 8, 28 -- X-Cam-SpamDetails: not scanned 8, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 8, 28 -- Received: from hermes-1.csi.cam.ac.uk ([131.111.8.51]:48126) 8, 28 -- by ppsw-41.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25) 8, 28 -- with esmtpa (EXTERNAL:jsb43) id 1Q5d9d-0003Zl-Rv (Exim 4.72) 8, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 01 Apr 2011 13:08:41 +0100 8, 28 -- Received: from prayer by hermes-1.csi.cam.ac.uk (hermes.cam.ac.uk) 8, 28 -- with local (PRAYER:jsb43) id 1Q5d9d-0007fh-Ks (Exim 4.67) 8, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 01 Apr 2011 13:08:41 +0100 8, 28 -- Received: from [131.111.102.31] by webmail.hermes.cam.ac.uk 8, 28 -- with HTTP (Prayer-1.3.3); 01 Apr 2011 13:08:41 +0100 8, 28 -- Date: 01 Apr 2011 13:08:41 +0100 8, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 8, 28 -- To: marcela.redigolo-at-mail.wvu.edu 8, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 8, 28 -- Subject: Re: Diffraction w/ Gatan SC600 TEM J2100 8, 28 -- Message-ID: {Prayer.1.3.3.1104011308410.26330-at-hermes-1.csi.cam.ac.uk} 8, 28 -- In-Reply-To: {201103311928.p2VJS6Zx031286-at-ns.microscopy.com} 8, 28 -- References: {201103311928.p2VJS6Zx031286-at-ns.microscopy.com} 8, 28 -- X-Mailer: Prayer v1.3.3 8, 28 -- Mime-Version: 1.0 8, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 8, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jzheng-at-calit2.uci.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hi, I would appreciate it if you might tell me your advice about good software for particle size analysis. I used to use Olympus-sis software. It seems to me that this company has reorganized. I could not contact the engineers who used to work for the company. Thanks, Jian-Guo Login Host: 68.5.37.248 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 1 07:19:38 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p31CJcQA028535 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:19:38 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 4C53D36D6E5 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:19:38 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id g2bT2ZAQS9Et for {microscopy-at-microscopy.com} ; 7, 25 -- Fri, 1 Apr 2011 07:19:35 -0500 (CDT) 7, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id 7575636D6D5 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:19:35 -0500 (CDT) 7, 25 -- Message-ID: {4D95C2D6.3030707-at-microscopy.com} 7, 25 -- Date: Fri, 01 Apr 2011 07:19:34 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: jzheng-at-calit2.uci.edu 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Particle analysis software 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Our JEOL 6060LV SEM quickly pumps to a verified 3x10 -5 Torr, but fails to indicate readiness to turn on the gun. We suspect its hardware needs adjustment somewhere on the front panel of boards. We have ordered schematics from JEOL for $150 but it may be awhile before they arrive. Could someone send us a diagram or hint so that we might be up and running again for our students? Thanks,
Kurt Carlson, Micro/Nano Fabrication Technician Chippewa Valley Technical College - Manufacturing Education Center NanoRite Innovation Center 715-874-4616 715-874-4603-Fax
==============================Original Headers============================== 9, 38 -- From kcarlson15-at-cvtc.edu Fri Apr 1 12:45:52 2011 9, 38 -- Received: from emf1.cvtc.edu (emf.cvtc.edu [160.32.2.121]) 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p31HjqLQ013262 9, 38 -- for {Microscopy-at-Microscopy.Com} ; Fri, 1 Apr 2011 12:45:52 -0500 9, 38 -- X-ASG-Debug-ID: 1301681213-03ab0c6f0001-JFxeax 9, 38 -- Received: from S-XCH-CAS-1.cvtc.local (s-xch-cas-1.cvtc.local [172.30.6.131]) by emf1.cvtc.edu with ESMTP id p5EJnEADhyE6DnoD for {Microscopy-at-Microscopy.Com} ; Fri, 01 Apr 2011 13:06:53 -0500 (CDT) 9, 38 -- X-Barracuda-Envelope-From: kcarlson15-at-cvtc.edu 9, 38 -- X-Barracuda-RBL-Trusted-Forwarder: 172.30.6.131 9, 38 -- X-ASG-Whitelist: Client 9, 38 -- Received: from s-xch-mbx-01.cvtc.local ([::1]) by S-XCH-CAS-1.cvtc.local 9, 38 -- ([::1]) with mapi; Fri, 1 Apr 2011 12:45:51 -0500 9, 38 -- From: "Carlson, Kurt" {kcarlson15-at-cvtc.edu} 9, 38 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 9, 38 -- Date: Fri, 1 Apr 2011 12:45:49 -0500 9, 38 -- X-ASG-Orig-Subj: SEM: need schematics for a JEOL 6060 9, 38 -- Subject: SEM: need schematics for a JEOL 6060 9, 38 -- Thread-Topic: SEM: need schematics for a JEOL 6060 9, 38 -- Thread-Index: AcvwlKNqfDcfCaaJReibMm9OdNgZZQ== 9, 38 -- Message-ID: {2D7DBE041245CC48A1D9A50883C39369C5D064C3-at-s-xch-mbx-01.cvtc.local} 9, 38 -- Accept-Language: en-US 9, 38 -- Content-Language: en-US 9, 38 -- X-MS-Has-Attach: 9, 38 -- X-MS-TNEF-Correlator: 9, 38 -- x-cr-hashedpuzzle: AN/s AvDQ A3Cb BK0W BP/9 CBec ChBl ClHO DJBE EwO3 FABX 9, 38 -- FXw2 Fhhd GVv9 HjdO 9, 38 -- J6HV;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{41DA1576-8931-417C-8630-E341583B9B35};awBjAGEAcgBsAHMAbwBuADEANQBAAGMAdgB0AGMALgBlAGQAdQA=;Fri, 9, 38 -- 01 Apr 2011 17:45:49 9, 38 -- GMT;UwBFAE0AOgAgAG4AZQBlAGQAIABzAGMAaABlAG0AYQB0AGkAYwBzACAAZgBvAHIAIABhACAASgBFAE8ATAAgADYAMAA2ADAA 9, 38 -- x-cr-puzzleid: {41DA1576-8931-417C-8630-E341583B9B35} 9, 38 -- acceptlanguage: en-US 9, 38 -- Content-Type: text/plain; charset="us-ascii" 9, 38 -- MIME-Version: 1.0 9, 38 -- X-Barracuda-Connect: s-xch-cas-1.cvtc.local[172.30.6.131] 9, 38 -- X-Barracuda-Start-Time: 1301681213 9, 38 -- X-Barracuda-URL: http://emf.cvtc.edu:8000/cgi-mod/mark.cgi 9, 38 -- X-Virus-Scanned: by bsmtpd at cvtc.edu 9, 38 -- Content-Transfer-Encoding: 8bit 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p31HjqLQ013262 ==============================End of - Headers==============================
MRC ANATOMICAL NEUROPHARMACOLOGY UNIT DEPARTMENT OF PHARMACOLOGY UNIVERSITY OF OXFORD, UK
Imaging Research Technician
Full time 36 hours/week Band 4 Salary from £26,022 - £31,758 per annum
An Imaging Research Technician is required to manage and provide user support for the IT and associated imaging equipment of the ANU scientific programmes relating to the basal ganglia the objective of which is to understand the principles of operation of the neuronal networks of the basal ganglia in health and disease. Bolam Lab web page: {http://mrcanu.pharm.ox.ac.uk/groups/paul.html} Magill Lab web page: {http://mrcanu.pharm.ox.ac.uk/groups/pete.html}
Evidence of previous microscopy experience and digital imaging work is essential. The post holder will also be required to perform histological processing and digital reconstruction of labelled neurons, train others in microscopic techniques and support the research programme through laboratory maintenance, ordering supplies and preparing reagents. Experience of website programming would be an advantage.
This is a permanent post with an initial probationary period. Salary range £26,022 - £31,758 per annum in the MRC band 4 range, depending on experience. We also offer optional membership of MRC pension scheme and 30 days annual leave.
Candidates holding an appropriate degree and with relevant experience should apply online at {https://ext.ssc.rcuk.ac.uk/} using post reference IRC18712. Please ensure you include a copy of your CV, covering letter and contact details for three professional referees within your application. If you do not have internet access or experience technical difficulties, please contact 01793 867003 quoting reference number IRC18712
For further information about the MRC please visit {http://www.mrc.ac.uk}
Closing date for applications Thursday 28th April 2011
The MRC is an Equal Opportunities Employer. Final appointments will be subject to pre-employment screening.
} } } } } } } }
Ben Micklem
-- Imaging Technician MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271866 {http://mrcanu.pharm.ox.ac.uk/}
==============================Original Headers============================== 13, 29 -- From ben.micklem-at-pharm.ox.ac.uk Fri Apr 1 12:46:13 2011 13, 29 -- Received: from relay4.mail.ox.ac.uk (relay4.mail.ox.ac.uk [129.67.1.163]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p31HkC46013569 13, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 12:46:13 -0500 13, 29 -- Received: from smtp1.nexus.ox.ac.uk ([163.1.154.135] helo=exht01.ad.oak.ox.ac.uk) 13, 29 -- by relay4.mail.ox.ac.uk with esmtp (Exim 4.74) 13, 29 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 13, 29 -- id 1Q5iQG-0005aF-E0 13, 29 -- for Microscopy-at-microscopy.com; Fri, 01 Apr 2011 18:46:12 +0100 13, 29 -- Received: from EXMBX04.ad.oak.ox.ac.uk ([169.254.1.123]) by 13, 29 -- exht01.ad.oak.ox.ac.uk ([163.1.154.52]) with mapi; Fri, 1 Apr 2011 18:46:12 13, 29 -- +0100 13, 29 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 13, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 29 -- Date: Fri, 1 Apr 2011 18:45:06 +0100 13, 29 -- Subject: Neuroscience imaging position available in Oxford 13, 29 -- Thread-Topic: Neuroscience imaging position available in Oxford 13, 29 -- Thread-Index: AcvwlLDLrmm5tD59RAaXx7tAZuPeUw== 13, 29 -- Message-ID: {C9BBCDB2.10C0A%ben.micklem-at-pharm.ox.ac.uk} 13, 29 -- Accept-Language: en-US, en-GB 13, 29 -- Content-Language: en-US 13, 29 -- X-MS-Has-Attach: 13, 29 -- X-MS-TNEF-Correlator: 13, 29 -- user-agent: Microsoft-MacOutlook/14.2.0.101115 13, 29 -- acceptlanguage: en-US, en-GB 13, 29 -- Content-Type: text/plain; charset="iso-8859-1" 13, 29 -- MIME-Version: 1.0 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p31HkC46013569 ==============================End of - Headers==============================
You are right, Olympus-SIS has reorganized in the US. The light microscopy applications (software, cameras and other hardware) was transferred to Olympus America, the Electron Microscopy Products were transferred to ResAlta Research Technologies (http://www.ResAltaTech.com). Please feel free to contact me at Mike.Bode-at-ResAltaTech.com, or call me for further information.
I am sorry you could not find the information about this reorganization.
Mike
--- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, April 01, 2011 6:26 AM To: mike.bode-at-resaltatech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jzheng-at-calit2.uci.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hi, I would appreciate it if you might tell me your advice about good software for particle size analysis. I used to use Olympus-sis software. It seems to me that this company has reorganized. I could not contact the engineers who used to work for the company. Thanks, Jian-Guo Login Host: 68.5.37.248 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Apr 1 07:19:38 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p31CJcQA028535 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:19:38 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 4C53D36D6E5 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:19:38 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id g2bT2ZAQS9Et for {microscopy-at-microscopy.com} ; 7, 25 -- Fri, 1 Apr 2011 07:19:35 -0500 (CDT) 7, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id 7575636D6D5 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 1 Apr 2011 07:19:35 -0500 (CDT) 7, 25 -- Message-ID: {4D95C2D6.3030707-at-microscopy.com} 7, 25 -- Date: Fri, 01 Apr 2011 07:19:34 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: jzheng-at-calit2.uci.edu 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Particle analysis software 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both james.passmore-at-sealedair.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: james.passmore-at-sealedair.com Name: Jim Passmore
Organization: Sealed Air Corporation
Title-Subject: [Filtered] LM shopping for microscopes
Message: Hello all,
I have the rare opportunity to spend some money, and am shopping for a couple of items. My applications are generally polymer materials, particularly packaging films. I'm asking the following--
Stereo Microscope: Each manufacturer seems to have several bodies available with different zoom ranges. Some claim you need electronic control to get good results on the higher zoom range/higher magnification configurations. Others claim great results with a manual 16X zoom. We'd like to keep things simple, but like the flexibility of a higher zoom. Opinions? Also, are there other disadvantages to a large zoom range?
Confocal microscope, primarily for surface metrology, with some other miscellaneous applications (no fluorescence): Big question here is the pros & cons of the different types of scopes--laser vs. white light. In addition, we're wondering about maintenance and reliability, as the parts get expensive.
General discussion can go to the list, but please keep manufacturer specifics off the list, of course.
Jim Passmore Research Associate Sealed Air Corporation
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mraderma-at-uvm,edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mraderma-at-uvm,edu Name: Michael Radermacher
Organization: University of Vermont
Title-Subject: [Filtered] 5th UVM Practical Course on 3D Cryo EM of Single Particles
Message: We are happy to announce the
5th UVM Practical Course on Three-dimensional Cryo Electron Microscopy of Single Particles July 24-31 2011, University of Vermont, Burlington, VT
This international course will teach the principles of three-dimensional reconstruction of single particles from cryo electron micrographs. The course will include in-depth teaching of the basic theoretical principles of single particle analysis, discussions and demonstrations of experimental procedures, and six hours per day of hands-on processing of data sets from electron micrographs of single particles. Participants will work in groups of two. Each group will have one instructor, who will provide step-by-step guidance through the complete three-dimensional reconstruction process and offer additional explanations and support. The course aims to provide participants with a strong practical and theoretical background that will enable them later to use these techniques in their home laboratory.
Application deadline: April 15th For details please visit: http://physioweb.med.uvm.edu/Cryo_Practical/
(late applications may be accepted if space is available) _________________________________ Michael Radermacher, PhD Teresa Ruiz, PhD University of Vermont College of Medicine Dept. Mol. Physiol. and Biophysics 149 Beaumont Ave Burlington, VT 05405
I'm afraid this vacuum is not good enough to obtain ready signal. It's better to look for vacuum leak (checking O-rings for example) or pumping problem. If your vacuum stays like that you will change often filament and get oil on the chamber. As I remenber there is a potentiometer to adjust ready level on the EVAC I/O board of the microscope but it should be ajusted only when the SEM can reach a good vacuum (best value around 4X10 -6 mbar).
-- Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung
kcarlson15-at-cvtc.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Our JEOL 6060LV SEM quickly pumps to a verified 3x10 -5 Torr, but fails to indicate readiness to turn on the gun. We suspect its hardware needs adjustment somewhere on the front panel of boards. We have ordered schematics from JEOL for $150 but it may be awhile before they arrive. Could someone send us a diagram or hint so that we might be up and running again for our students? } Thanks, } } Kurt Carlson, Micro/Nano Fabrication Technician } Chippewa Valley Technical College - Manufacturing Education Center } NanoRite Innovation Center } 715-874-4616 } 715-874-4603-Fax } } } } }
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Mon Apr 4 07:35:28 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p34CZRHm028912 8, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 4 Apr 2011 07:35:28 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p34CZM9G032115; 8, 25 -- Mon, 4 Apr 2011 14:35:22 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p34CZISX021413 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Mon, 4 Apr 2011 14:35:19 +0200 8, 25 -- Message-ID: {4D99BD41.1030601-at-univ-nantes.fr} 8, 25 -- Date: Mon, 04 Apr 2011 14:44:49 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: kcarlson15-at-cvtc.edu, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] SEM: need schematics for a JEOL 6060 8, 25 -- References: {201104011759.p31HxVhI011743-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201104011759.p31HxVhI011743-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4D99BB0A.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4D99BB0A.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
Does anyone have any opinions on whether resin blocks deteriorate over time (i.e. decades), and whether some storage options (airtight, low temperature...?) are more appropriate than others?
This has implications for type specimens deposited in museums, where the primary description of a species is based on TEM. Singe-celled eukaryotes often have no other useful type material. Anecdotally, preservation of ultrastructure in some blocks from the 1960s has deteriorated since they were looked at in the 1980s - but it's unclear if this is a general pattern.
thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
Looks like we have to surplus our Hitachi H-7000 FA TEM: We have a new JEOL, and someone else has claimed the lab space for the H-7000 starting in fall 2011, so I'm putting it up for sale. You can find details at: http://ahrenkiel.sdsmt.edu/H7000_TEM/. Please go to the link for "surplus" at the bottom of the page.
I'd prefer to sell the whole thing together, but am willing to discuss parting it out. A bevy of accessories are included - The EDX system (minus the detector) is practically new. Don't feel timid to make an offer.
The annual service contract on this model is relatively inexpensive. I wish I could keep both microscopes, but letting go of the things we are attached to is part of the cycle of life. Hopefully, there is a nice, new home for it out there somewhere. ------------------------------------------ Phil Ahrenkiel, Assistant Professor Nanoscience and Nanoengineering Ph.D. Program South Dakota School of Mines and Technology 501 E. Saint Joseph St. Rapid City, SD 57701 U.S.A. Office: EP 221 Phone: 605-394-5238, Fax: 605-394-2365 Email: Phil.Ahrenkiel-at-sdsmt.edu
Are you thinking of deterioration of resin over time? Polymerization may not be completely over and done with within a day or two, but continue more and more slowly during storage. Also UV light exposure should probably be avoided. All this would make the blocks more brittle over time, by reducing chin length or breaking bonds. But there is also the risk that any of the used chemicals (e.g. OsO4) is not completely inactive or thoroughly washed out before embedding. And there is not much one can do afterwards...
I would therefore choose conditions that reduce reaction speed (Q10) in general, i.e. relatively low temperature storage away from direct light.
Cheers
Jan
Jan Leunissen Aurion
On 5/04/2011, at 11:12 AM, giselle.walker-at-anatomy.otago.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Does anyone have any opinions on whether resin blocks deteriorate over time (i.e. decades), and whether some storage options (airtight, low temperature...?) are more appropriate than others? } } This has implications for type specimens deposited in museums, where the primary description of a species is based on TEM. Singe-celled eukaryotes often have no other useful type material. Anecdotally, preservation of ultrastructure in some blocks from the 1960s has deteriorated since they were looked at in the 1980s - but it's unclear if this is a general pattern. } } thanks } } Giselle } } -- } Dr Giselle Walker } Microscopy Otago - Electron Microscopy } Department of Anatomy & Structural Biology } University of Otago } P.O. Box 913, Dunedin 9054 } New Zealand } } Phone: +64 (0)3 479 7301, +64 (0)210 403 669 } Email: giselle.walker-at-anatomy.otago.ac.nz } } } } } } } ==============================Original Headers============================== } 11, 30 -- From giselle.walker-at-anatomy.otago.ac.nz Mon Apr 4 18:12:00 2011 } 11, 30 -- Received: from n1b.bullet.mail.aue.yahoo.com (n1b.bullet.mail.aue.yahoo.com [124.108.96.250]) } 11, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p34NBxVc004328 } 11, 30 -- for {microscopy-at-microscopy.com} ; Mon, 4 Apr 2011 18:12:00 -0500 } 11, 30 -- Received: from [124.108.96.171] by n1.bullet.mail.aue.yahoo.com with NNFMP; 04 Apr 2011 23:11:58 -0000 } 11, 30 -- Received: from [124.108.96.186] by t2.bullet.mail.aue.yahoo.com with NNFMP; 04 Apr 2011 23:11:58 -0000 } 11, 30 -- Received: from [127.0.0.1] by omp101.mail.aue.yahoo.com with NNFMP; 04 Apr 2011 23:11:58 -0000 } 11, 30 -- X-Yahoo-Newman-Id: 438068.58289.bm-at-omp101.mail.aue.yahoo.com } 11, 30 -- Received: (qmail 9065 invoked by uid 1000); 4 Apr 2011 23:11:57 -0000 } 11, 30 -- Received: from 124.108.96.71 by rel107.mail.aue.yahoo.com with SMTP; Mon, 04 Apr 2011 16:11:57 -0700 } 11, 30 -- Received: (qmail 90499 invoked from network); 4 Apr 2011 23:11:57 -0000 } 11, 30 -- Received: from giselle.otago.ac.nz (giselle.walker-at-139.80.34.105 with plain) } 11, 30 -- by smtp102.tnz.mail.aue.yahoo.com with SMTP; 04 Apr 2011 16:11:57 -0700 PDT } 11, 30 -- X-Yahoo-SMTP: jK_f8jSswBAS_SUBAvuGpAHlV1.qp_ZG_Fc0IUTfbFAOOFlUVS0- } 11, 30 -- X-YMail-OSG: FzsAulMVM1nZCBtXmvDK71ftIYLBTOIGFn5TPaKryySSUfR } 11, 30 -- r6349umNneFEL_ayQ9.NHDM6xH9PjPdy7SptFOUJ7a8wWRxFHhPuHcNXp.8w } 11, 30 -- Z5JV2qSIv4k1ev0xOXZKvsBsZHP1SecR0tfGsCAAl4Wx1VAo3IsxTgHE.6VB } 11, 30 -- 6WSCJiC0eDe599CWV9HO8D8rIY_snA8ioN613YIv2HZWVuAamVmQs5Y31I6D } 11, 30 -- zpOVYoDoRfsJ2Mu0iUA-- } 11, 30 -- X-Yahoo-Newman-Property: ymail-3 } 11, 30 -- From: Giselle Walker {giselle.walker-at-anatomy.otago.ac.nz} } 11, 30 -- Content-Type: text/plain; charset=us-ascii } 11, 30 -- Subject: long-term storage of resin blocks for TEM } 11, 30 -- Date: Tue, 5 Apr 2011 11:11:56 +1200 } 11, 30 -- Message-Id: {15B2B5B6-EFC8-4573-9980-F1942F4C56A7-at-anatomy.otago.ac.nz} } 11, 30 -- To: microscopy-at-microscopy.com } 11, 30 -- Mime-Version: 1.0 (Apple Message framework v1082) } 11, 30 -- X-Mailer: Apple Mail (2.1082) } 11, 30 -- Content-Transfer-Encoding: 8bit } 11, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p34NBxVc004328 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 21 -- From leunissen-at-aurion.nl Mon Apr 4 19:10:44 2011 11, 21 -- Received: from fep02.xtra.co.nz (fep02.xtra.co.nz [210.54.141.244]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p350AgMq005159 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 4 Apr 2011 19:10:43 -0500 11, 21 -- Received: from unknown-d8-a2-5e-8d-da-93.lan ([122.57.245.79]) 11, 21 -- by fep02.xtra.co.nz with ESMTP 11, 21 -- id {20110405001040.PXEU9811.fep02.xtra.co.nz-at-unknown-d8-a2-5e-8d-da-93.lan} ; 11, 21 -- Tue, 5 Apr 2011 12:10:40 +1200 11, 21 -- Subject: Re: [Microscopy] long-term storage of resin blocks for TEM 11, 21 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 21 -- Content-Type: text/plain; charset=us-ascii 11, 21 -- From: Jan Leunissen - Aurion {leunissen-at-aurion.nl} 11, 21 -- In-Reply-To: {201104042312.p34NCGn0004622-at-ns.microscopy.com} 11, 21 -- Date: Tue, 5 Apr 2011 12:10:40 +1200 11, 21 -- Cc: microscopy-at-microscopy.com 11, 21 -- Message-Id: {75BCA6E3-0D20-4F14-A71E-C2F6C9AF5D53-at-aurion.nl} 11, 21 -- References: {201104042312.p34NCGn0004622-at-ns.microscopy.com} 11, 21 -- To: giselle.walker-at-anatomy.otago.ac.nz 11, 21 -- X-Mailer: Apple Mail (2.1084) 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p350AgMq005159 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both xiaohu.tang-at-beg.utexas.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Suggestion needed for carbon coater
Message: Dear listeners,
I am looking for a carbon coater for our lab but feel difficult to choose from such as Emitech, Ted Pella, Denton Vaccum, SPI, or Cressington, etc. Since we are not working on very high mag so carbon coater is enough. Most of our samples are thin-section rocks. What we need is a stable, robust, not too expensive and with good service. You will be highly appreciated if you could give your advice and share with your experience on these brands carbon coater. You can also send it to my email xiaohu.tang-at-beg.utexas.edu
I just sectioned blocks from 1976 two weeks ago. The blocks cut fine, with the exception that some areas had not been infiltrated properly when they were initially processed. The tissue was of sea slug that was rare and irreplacable. We got the information from the blocks that we needed. The plastic was epon-araldite. Tissue preservation was perfect in properly embedded areas, and the plastic was stable in the beam. If the samples are prepared properly in the first place, they are permanently archived in the plastic for a long, long time. Store the blocks in a climate-controlled area. The blocks I cut were stored in someone's attic. I was surprised that they survived the harsh heat and cold in that environment! I have sections that I cut, still on grids, from 1974, that I can put in the TEM and photograph today. If the sections are stored properly, they are archival, too. They are not on carbon or formvar films, just bare copper grids that have been kept in a low humidity environment all this time. I have re-scoped them several times before. It's been my experience that tissue deterioration in blocks comes from improper dehydration or infiltration during processing. If the samples were run up properly in the first place, the tissue is archival. It is possible, and I have seen this, to have a processing schedule that is barely usable, that just gets a person by. This produces tissue where the osmium is not fully washed out, or the tissue is not fully dehydrated, and the blocks are either soft or brittle. You may be able to cut the tissue initially, but the blocks deteriorate with time. Well processed tissue will not do this. The processing problems usually occur when trying to process tissue that is too large, or when running something up too fast, or when using old chemicals. That, at least, has been my experience. You may have come to similar conclusions. Epoxy blocks commonly used for E.M. should be archival. Lowicryls and LR White and Gold may pick up moisture with time and deteriorate. They shou! ld also be protected from UV light, since most of the work with these resins is for immunogold labeling, and antigenic preservation is the goal. I always remove my blocks from block molds. I don't know if the plastic from the mold will interact with the tissue or epoxy in the block with time.
Ed Haller
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: giselle.walker-at-anatomy.otago.ac.nz [giselle.walker-at-anatomy.otago.ac.nz] Sent: Monday, April 04, 2011 7:20 PM To: Haller, Edward
Does anyone have any opinions on whether resin blocks deteriorate over time (i.e. decades), and whether some storage options (airtight, low temperature...?) are more appropriate than others?
This has implications for type specimens deposited in museums, where the primary description of a species is based on TEM. Singe-celled eukaryotes often have no other useful type material. Anecdotally, preservation of ultrastructure in some blocks from the 1960s has deteriorated since they were looked at in the 1980s - but it's unclear if this is a general pattern.
thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arjay007-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arjay007-at-gmail.com Name: Ritchie Sims
Organization: The University of Auckland
Title-Subject: [Filtered] Vacuum problems on JEOL 840
Message: Hi I switched the 840 off while on vacation, and now I have vacuum problems. With manual control of the valves, I can't get the RP to get the DP pressure down to where it has been for years. I have a 100uA meter connected to the PG3 test points down on the bottom RH corner of the vacuum control board, usually this sits at about 10uA (where 70 or 80 is about the level for the PiG3 warning LED to come on) but now I can't get it below 45uA. The pressure is down low enough to switch PiG3 LED off, but I really really don't want to risk cooking the DP Santovac. Finding out what's going on is hampered by (a) the timer that switches the whole thing off after 20minutes and (b) the fact that I don't really know what backing pressure I am actually getting down to.
Four questions:
(a) How can I defeat the 20-minute switchoff timer so that I can at least let the RP have a decent long suck on the (cold) DPs to see if it will come right given enough time?
(b) What does the backing pressure have to get down to in order to avoid not cooking the Santovac?
(c) Is it ok, after separately pumping the chamber, the gun, and the DPs down to about 1 x 10-1 mbar, to then manually open the valves V2, V1A, and V1B ie the valves as they are usually in the 'pumpdown' state, but with the DPs cold? At least then I would know what backing pressure I was getting down to, as I have 'proper' vacuum gauges on both the gun and the chamber. Maybe it is OK to turn on the DP heaters, but I'm reluctant to do so until I know what my backing pressure is.
(d) Anyone got any suggestions or comments as to what might be going on?
cheers Ritchie Sims (the listserver rejects postings from me as myself)
Hello listservers, I recently had embedding issues with Drosophila eyes where some pigment granules were not infiltrated (resulting in holes). I had tried extended parts of both Epon and Spurrs to propylene but some heads would not infiltrate. I came upon Durcupan through a recent Science cover,and tried a run with much success. Although I did dehydrate and transition with propylene oxide (to get maximum infiltration), I read in the product description that Component A is water miscible, and that you could avoid both ETOH and transition solvents by dehydrating with it.
My questions are has anyone tried this and gotten successful infiltration (not only insects but all types of tissues or cells)? What does the dehydration (in Component A) schedule look like? Shouldn't this be the norm to avoid shrinkage and extraction by these solvents? Are there any drawbacks?
P.S. LR White embedding was also used but the orientation was hit or miss as embedding must be done in gelatin capsules.
Thank you,
Michael Delannoy Associate Director JHMI Microscope Facility Physiology G-04 725 N. Wolfe St. Baltimore, MD 21205
(410) 955-1365
==============================Original Headers============================== 8, 29 -- From prvs=069d7c001=delannoy-at-jhmi.edu Tue Apr 5 08:41:39 2011 8, 29 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p35DfdLc027623 8, 29 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 08:41:39 -0500 8, 29 -- X-IronPort-AV: E=Sophos;i="4.63,304,1299474000"; 8, 29 -- d="scan'208";a="91880170" 8, 29 -- Received: from jhem01.johnshopkins.edu (HELO commsmail.johnshopkins.edu) ([10.173.7.11]) 8, 29 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 05 Apr 2011 09:41:38 -0400 8, 29 -- MIME-version: 1.0 8, 29 -- Content-transfer-encoding: 7BIT 8, 29 -- Content-disposition: inline 8, 29 -- Content-type: text/plain; CHARSET=US-ASCII 8, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.15]) 8, 29 -- by commsmail.johnshopkins.edu 8, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 8, 29 -- with ESMTP id {0LJ600G4PM1EFV00-at-commsmail.johnshopkins.edu} for 8, 29 -- microscopy-at-microscopy.com; Tue, 05 Apr 2011 09:41:38 -0400 (EDT) 8, 29 -- Received: from [10.173.109.15] (Forwarded-For: [10.16.66.49]) 8, 29 -- by commsmail.johnshopkins.edu (mshttpd); Tue, 05 Apr 2011 09:41:38 -0400 8, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 8, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 29 -- Message-id: {7300cd241a771.4d9ae3d2-at-johnshopkins.edu} 8, 29 -- Date: Tue, 05 Apr 2011 09:41:38 -0400 8, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 8, 29 -- 2009) 8, 29 -- Content-language: en 8, 29 -- Subject: Durcupan 8, 29 -- X-Accept-Language: en 8, 29 -- Priority: normal ==============================End of - Headers==============================
Several years ago I sectioned blocks that were about 20 years old and they were perfect. These were ones that had not been previously used or trimmed. They had been kept in thin cardboard pill boxes inside 8X10 inch Kodak photographic paper boxes in a glass door cabinet. Humidity in the summer in Philadelphia is usually very high so I¹d say that the lab ran about 80% even with the air conditioning on. Temperature in the lab would range from near 65 most of the winter (trouble with heating system in our ancient building) to high-70's in the summer.
To my knowledge these blocks would have been made after I had run out of Epon 812 (original formula from Shell, for the younger members of our Listserv) but they just may have been embedded with the ³good stuff² towards the end of my supply. The blocks were cured at 60 C for a minimum of 48 hours if not over a weekend.
You mentioned that your blocks were from the 60's. That was still a time when Epon 812 was still the embedding of choice for many labs but there were also many other embeddings being used like Araldite/Epon mixtures with which I am unfamiliar.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
I was hoping to get some expert advice about a job description. I have included the job description below. I would like receive feedback as to what a person who fulfills these responsibilities would be hired at (i.e. Core Director, Core Manager, Research Associate 1, etc.). I would also like to know what you would consider the highest level of education you think would be needed for this position. I realize the job description is quite long, but I appreciate any feedback.
Thank You.
Essential Duties and Responsibilities
1. Preparation of specimens such as paraffin, EM, hard tissue embedding and embedding frozen tissue in OCT compound. 2. EM, hard tissue, paraffin and frozen (cryosectioning) tissue sectioning. 3. Operate a Zeiss 10 Transmission Electron Microscope and Gatan 785 Digital Imaging system. 4. Operate a Zeiss LSM 510 and Pascal Confocal Microscope. 5. Operate a Zeiss Axio Observer, 21 Inverted Microscope with FRET, transmitted brightfield, DTC and Epi-fluorescence capabilities. 6. Requisitioning and Ordering supplies. 7. Maintains Equipment/supplies/Facilities: Maintains the laboratory facilities and equipment and controls supplies. Ensures that a sufficient supply of reagents, standards and controls are available at all times by ordering an adequate supply from commercial sources or preparing variety of specialized solutions or reagents. Using established procedures, calibrates, controls, and maintains a variety of instrumentation and laboratory equipment. When calibrations fall outside the acceptable limits, incumbent uses independent judgment in adjusting either the procedure or the mechanical device used in the determination to bring the procedures into good quality control. Plus general lab cleaning. 8. Customer Services: In accordance with Service procedures, answers the phone promptly in a courteous manner, identifying self and service. Similarly, outgoing calls are handled appropriately and politely. Valid complaints about failure to do so are not normally received by his/her supervisor. Shows positive evidence of a helpful attitude to other staff, visitors and clients through such actions as a consistent display of courtesy. Success in the area is measured by observation plus evaluation of any valid complaints and or commendations received during the rating period. Promotes attitude and procedures that foster both internal and external customer satisfaction. 9. Security: Incumbent is responsible for maintaining security of information in computerized systems in accordance with medial center policy: the Privacy Act; and other applicable laws, rules, regulations, and policies. 10. Safety: Inspect laboratory space for safe conditions and conformance to safety procedure. 11. Prepare core facilities invoices for jobs completed.
==============================Original Headers============================== 9, 37 -- From prvs=1076151b73=mmashore-at-vapop.ucsd.edu Tue Apr 5 13:27:07 2011 9, 37 -- Received: from iport-c2-out.ucsd.edu (iport-c2-out.ucsd.edu [132.239.0.119]) 9, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p35IR6Jd001882 9, 37 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 13:27:06 -0500 9, 37 -- X-IronPort-Anti-Spam-Filtered: true 9, 37 -- X-IronPort-Anti-Spam-Result: AuMGACFem02E71Ri/2dsb2JhbACKG45HjQl3tg+EW4hthWwEhUc 9, 37 -- X-IronPort-AV: E=Sophos;i="4.63,305,1299484800"; 9, 37 -- d="scan'208";a="472283752" 9, 37 -- X-Spam-Status: No 9, 37 -- X-Spam-Level: 9, 37 -- Received: from vapop.ucsd.edu ([132.239.84.98]) 9, 37 -- by iport-c2-out.ucsd.edu with ESMTP; 05 Apr 2011 11:27:06 -0700 9, 37 -- Received: from vandeberg by vapop.ucsd.edu (MDaemon PRO v9.6.6) 9, 37 -- with ESMTP id md50002136612.msg 9, 37 -- for {Microscopy-at-Microscopy.Com} ; Tue, 05 Apr 2011 11:25:27 -0700 9, 37 -- X-Spam-Processed: vapop.ucsd.edu, Tue, 05 Apr 2011 11:25:27 -0700 9, 37 -- (not processed: message from trusted or authenticated source) 9, 37 -- X-Return-Path: prvs=1076151b73=mmashore-at-vapop.ucsd.edu 9, 37 -- X-Envelope-From: mmashore-at-vapop.ucsd.edu 9, 37 -- X-MDaemon-Deliver-To: Microscopy-at-Microscopy.Com 9, 37 -- From: "Michael Mashore" {mmashore-at-vapop.ucsd.edu} 9, 37 -- To: {Microscopy-at-microscopy.com} 9, 37 -- Subject: Job Description Help 9, 37 -- Date: Tue, 5 Apr 2011 11:26:10 -0700 9, 37 -- Message-ID: {99E152F716D2426781505CFD90CC2E89-at-vandeberg} 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; 9, 37 -- charset="us-ascii" 9, 37 -- X-Priority: 3 (Normal) 9, 37 -- X-MSMail-Priority: Normal 9, 37 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 9, 37 -- Importance: Normal 9, 37 -- Thread-Index: Acvzvu/teNqgkOqxSuGGm4a7qM8oqg== 9, 37 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 9, 37 -- X-MDAV-Processed: vapop.ucsd.edu, Tue, 05 Apr 2011 11:25:28 -0700 9, 37 -- Content-Transfer-Encoding: 8bit 9, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p35IR6Jd001882 ==============================End of - Headers==============================
For anti-HA, we use HA.11 Clone16B12 Mouse Monoclonal AB Covance. It works pretty well for immunoflourescence and immunogold labelling.
For pre-embed immunogold labelling, we tried different fixative strengths and length of fixation and found overnight fixation with 4% formaldehyde (PFA) achieved adequate ultrastructure and optimal labelling. We had tried adding 0.1% or 1% glutaraldehyde but the labelling decreased and became less consistent.
For immunoflourescence labelling on thick cryosections, 3 hour 4% PFA was sufficient to get labelling and good, intact morphology under LM. I have not yet tried labelling thin cryosections for TEM on these samples but hopefully soon. But I feel we have a good headstart with our preliminary data.
Hopefully by now you have the vendor informations. Just in case, there's Aurion and Nanoprobes.
Good Luck,
Claire
Dear list, I need good antibodies (from different species, mouse and rabbit preferentially) against these two tags that would work for immunogold labeling on Tokuyasu cryosections. I think 6% formaldehyde, 4% formaldehyde/0.1% glutaraldehyde or 2% formaldehyde/0.2% glutaraldehyde would be the fixations I would try for the starters. Any recommendations? Also, recommendations needed on suppliers of gold conjugates. For the last couple of years I was only using protein A from a single source that does not offer antibodies, so I do not have current information. Thanks, Michal
==============================Original Headers============================== 9, 30 -- From chaueter-at-bcm.edu Tue Apr 5 15:45:24 2011 9, 30 -- Received: from iron1.corp.bcm.tmc.edu (iron1.corp.bcm.tmc.edu [128.249.224.13]) 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p35KjNFL021648 9, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 15:45:24 -0500 9, 30 -- X-IronPort-AV: E=Sophos;i="4.63,306,1299477600"; 9, 30 -- d="scan'208";a="112576556" 9, 30 -- Received: from unknown (HELO EPW-EXHUB2-P021.ad.bcm.edu) ([10.22.132.48]) 9, 30 -- by iron1-out.bcm.edu with ESMTP; 05 Apr 2011 15:45:22 -0500 9, 30 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 9, 30 -- EPW-EXHUB2-P021.ad.bcm.edu ([::1]) with mapi; Tue, 5 Apr 2011 15:45:22 -0500 9, 30 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 9, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 9, 30 -- Date: Tue, 5 Apr 2011 15:45:21 -0500 9, 30 -- Subject: Anti-HA and anti-myc tag antibodies for immunogold 9, 30 -- Thread-Topic: Anti-HA and anti-myc tag antibodies for immunogold 9, 30 -- Thread-Index: AQHL89JhYdxKYHirrEeUlJfw/Q8YJQ== 9, 30 -- Message-ID: {F3EEF3767DFF3244826E72285C1C273449E8965A69-at-EXCMSMBX03.ad.bcm.edu} 9, 30 -- Accept-Language: en-US 9, 30 -- Content-Language: en-US 9, 30 -- X-MS-Has-Attach: 9, 30 -- X-MS-TNEF-Correlator: 9, 30 -- acceptlanguage: en-US 9, 30 -- x-tm-as-product-ver: SMEX-8.0.0.1307-6.500.1024-18056.001 9, 30 -- x-tm-as-result: No--49.253500-8.000000-31 9, 30 -- x-tm-as-user-approved-sender: No 9, 30 -- x-tm-as-user-blocked-sender: No 9, 30 -- Content-Type: text/plain; charset="us-ascii" 9, 30 -- MIME-Version: 1.0 9, 30 -- Content-Transfer-Encoding: 8bit 9, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p35KjNFL021648 ==============================End of - Headers==============================
May I learn tips and tricks for fixation and processing of praying mantis egg cases for ultrastructure, both SEM and thin section TEM. A interest are the ootheca and not the embryos.
Thanks so much,
Steve
-- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
==============================Original Headers============================== 5, 18 -- From steven.samuelsson-at-sri.com Tue Apr 5 17:03:44 2011 5, 18 -- Received: from mail1.sri.com (srimail.SRI.COM [128.18.30.17]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p35M3iYH006994 5, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 17:03:44 -0500 5, 18 -- MIME-version: 1.0 5, 18 -- Content-transfer-encoding: 7BIT 5, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 5, 18 -- Received: from Steves-iMac.local ([unknown] [128.18.51.106]) 5, 18 -- by mail.sri.com (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built 5, 18 -- Jul 30 2009)) with ESMTP id {0LJ7002LC96PW750-at-mail.sri.com} for 5, 18 -- Microscopy-at-microscopy.com; Tue, 05 Apr 2011 15:01:37 -0700 (PDT) 5, 18 -- Message-id: {4D9B9141.8030002-at-sri.com} 5, 18 -- Date: Tue, 05 Apr 2011 15:01:37 -0700 5, 18 -- From: Steven Samuelsson {steven.samuelsson-at-sri.com} 5, 18 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.2.15) 5, 18 -- Gecko/20110303 Thunderbird/3.1.9 5, 18 -- To: Microscopy-at-microscopy.com 5, 18 -- Subject: EM of Mantis Ootheca ==============================End of - Headers==============================
I was wondering if anybody has had any experience with fast, large area (3-10mm), photon counters for FLIM acquisition. I was hoping that somebody might have used a B&H HPM-100-40 detector, and might be able to give me some feedback...
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arjay007-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arjay007-at-gmail.com Name: Ritchie Sims
Organization: The University of Auckland
Title-Subject: [Filtered] some progress with 840 vacuum
Message: Thanks to all who have replied.
I realise that my initial posting may not have been completely clear:
What I am having trouble with is getting the DPs down to a good roughing vacuum before switching on the heaters ie all this is with the DPs cold. In the past I have been able to get it down to 20uA (on the 100uA panel meter that I have attached to the PG3 test points) with no problems, but now it gets down only to 40uA, and although this is sufficient vacuum to extinguish the PiG3 LED (and the AUTO mode would presumably take this as good enough) I am somewhat un-nerved by the apparent change and too suspicious to switch on the DP heaters. Just now I have figured out, with the assistance of one of you, how to defeat the 20-minute timer. It is the timer that shuts down the system if the DPs have not warmed up within 20 minutes of startup, so defeating it is just a matter of jumpering the warmup thermal switches on the two DPs. This makes the HEATER LED turn off, and the timer (presumably IC 2A, a type 51845 about which I can find no information) no longer switches the system off. So I can leave the RP sucking on the cold DPs overnight, see if the vacuum improves.
And now I have had the courage to get the three system spaces down as far as I can (still with the DPs cold) and open all the valves that are usually open when it's in the "OK-to-use" state ie RP open to DPs, DPs open to gun and column. The gun vacuum gauge reads about 0.03 mbar (about 0.02 torr), so the backing pressure for the DPs must be at least as low as this. The question now is: Is 0.03mbar a good enough vacuum to switch on the DP heaters without running the risk of charring the Santovac?
all the best Ritchie
Message: Hi I switched the 840 off while on vacation, and now I have vacuum problems. With manual control of the valves, I can't get the RP to get the DP pressure down to where it has been for years. I have a 100uA meter connected to the PG3 test points down on the bottom RH corner of the vacuum control board, usually this sits at about 10uA (where 70 or 80 is about the level for the PiG3 warning LED to come on) but now I can't get it below 45uA. The pressure is down low enough to switch PiG3 LED off, but I really really don't want to risk cooking the DP Santovac. Finding out what's going on is hampered by (a) the timer that switches the whole thing off after 20minutes and (b) the fact that I don't really know what backing pressure I am actually getting down to.
Four questions:
(a) How can I defeat the 20-minute switchoff timer so that I can at least let the RP have a decent long suck on the (cold) DPs to see if it will come right given enough time?
(b) What does the backing pressure have to get down to in order to avoid not cooking the Santovac?
(c) Is it ok, after separately pumping the chamber, the gun, and the DPs down to about 1 x 10-1 mbar, to then manually open the valves V2, V1A, and V1B ie the valves as they are usually in the 'pumpdown' state, but with the DPs cold? At least then I would know what backing pressure I was getting down to, as I have 'proper' vacuum gauges on both the gun and the chamber. Maybe it is OK to turn on the DP heaters, but I'm reluctant to do so until I know what my backing pressure is.
(d) Anyone got any suggestions or comments as to what might be going on?
Hello Ritchie, going down with the roughing pumps to 0,02 torr is more than five times lower than you need for a good backing pressure of the diffusion pumps. Switch them on, pump down and be happy (if no other problem arises) :-)
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Wissenschaftliche Photographie Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 06.04.11 04:16, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both arjay007-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: arjay007-at-gmail.com Name: Ritchie Sims } } Organization: The University of Auckland } } Title-Subject: [Filtered] some progress with 840 vacuum } } Message: Thanks to all who have replied. } } I realise that my initial posting may not have been completely clear: } } What I am having trouble with is getting the DPs down to a good roughing } vacuum before switching on the heaters ie all this is with the DPs cold. } In the past I have been able to get it down to 20uA (on the 100uA panel } meter that I have attached to the PG3 test points) with no problems, but } now it gets down only to 40uA, and although this is sufficient vacuum to } extinguish the PiG3 LED (and the AUTO mode would presumably take this as } good enough) I am somewhat un-nerved by the apparent change and too } suspicious to switch on the DP heaters. } Just now I have figured out, with the assistance of one of you, how to } defeat the 20-minute timer. It is the timer that shuts down the system } if the DPs have not warmed up within 20 minutes of startup, so defeating } it is just a matter of jumpering the warmup thermal switches on the two } DPs. This makes the HEATER LED turn off, and the timer (presumably IC } 2A, a type 51845 about which I can find no information) no longer } switches the system off. So I can leave the RP sucking on the cold DPs } overnight, see if the vacuum improves. } } And now I have had the courage to get the three system spaces down as } far as I can (still with the DPs cold) and open all the valves that are } usually open when it's in the "OK-to-use" state ie RP open to DPs, DPs } open to gun and column. The gun vacuum gauge reads about 0.03 mbar } (about 0.02 torr), so the backing pressure for the DPs must be at least } as low as this. } The question now is: Is 0.03mbar a good enough vacuum to switch on the } DP heaters without running the risk of charring the Santovac? } } all the best } Ritchie } } } } Message: Hi } I switched the 840 off while on vacation, and now I have vacuum } problems. With manual control of the valves, I can't get the RP to get } the DP pressure down to where it has been for years. I have a 100uA } meter connected to the PG3 test points down on the bottom RH corner of } the vacuum control board, usually this sits at about 10uA (where 70 or } 80 is about the level for the PiG3 warning LED to come on) but now I } can't get it below 45uA. The pressure is down low enough to switch PiG3 } LED off, but I really really don't want to risk cooking the DP Santovac. } Finding out what's going on is hampered by (a) the timer that switches } the whole thing off after 20minutes and (b) the fact that I don't really } know what backing pressure I am actually getting down to. } } Four questions: } } (a) How can I defeat the 20-minute switchoff timer so that I can at } least let the RP have a decent long suck on the (cold) DPs to see if it } will come right given enough time? } } (b) What does the backing pressure have to get down to in order to avoid } not cooking the Santovac? } } (c) Is it ok, after separately pumping the chamber, the gun, and the DPs } down to about 1 x 10-1 mbar, to then manually open the valves V2, V1A, } and V1B ie the valves as they are usually in the 'pumpdown' state, but } with the DPs cold? At least then I would know what backing pressure I } was getting down to, as I have 'proper' vacuum gauges on both the gun } and the chamber. Maybe it is OK to turn on the DP heaters, but I'm } reluctant to do so until I know what my backing pressure is. } } (d) Anyone got any suggestions or comments as to what might be going on? } } } Login Host: 130.216.59.110 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 21, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 5 21:11:06 2011 } 21, 25 -- Received: from znl.com ([206.69.208.20]) } 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p362B6cO011233 } 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 21:11:06 -0500 } 21, 25 -- Received: from localhost (localhost [127.0.0.1]) } 21, 25 -- by znl.com (Postfix) with ESMTP id 6EF31373479 } 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 21:11:06 -0500 (CDT) } 21, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 21, 25 -- Received: from znl.com ([127.0.0.1]) } 21, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 21, 25 -- with ESMTP id X1gy8PI8fL-J for {microscopy-at-microscopy.com} ; } 21, 25 -- Tue, 5 Apr 2011 21:11:05 -0500 (CDT) } 21, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 21, 25 -- by znl.com (Postfix) with ESMTPA id 78EE537346E } 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 21:11:05 -0500 (CDT) } 21, 25 -- Message-ID: {4D9BCBB9.1040506-at-microscopy.com} } 21, 25 -- Date: Tue, 05 Apr 2011 21:11:05 -0500 } 21, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 21, 25 -- Reply-To: arjay007-at-gmail.com } 21, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 21, 25 -- MIME-Version: 1.0 } 21, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 21, 25 -- Subject: viaWWW:some progress with 840 vacuum } 21, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 21, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 22 -- From stefan.diller-at-t-online.de Wed Apr 6 01:33:19 2011 6, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p366XJF2031933 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 01:33:19 -0500 6, 22 -- Received: from fwd05.aul.t-online.de (fwd05.aul.t-online.de ) 6, 22 -- by mailout08.t-online.de with smtp 6, 22 -- id 1Q7MIn-0000y1-HT; Wed, 06 Apr 2011 08:33:17 +0200 6, 22 -- Received: from mac-pro.local (TW35w4ZLZho05bSSQcAx44B8+Yxg+AlDf8pPaM-8WD2kLFzjXsTO2DdD1JqWI8Eg1d-at-[80.17.62.163]) by fwd05.t-online.de 6, 22 -- with esmtp id 1Q7MIe-12l1600; Wed, 6 Apr 2011 08:33:08 +0200 6, 22 -- Message-ID: {4D9C0923.6040600-at-t-online.de} 6, 22 -- Date: Wed, 06 Apr 2011 08:33:07 +0200 6, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 22 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 6, 22 -- MIME-Version: 1.0 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] viaWWW:some progress with 840 vacuum 6, 22 -- References: {201104060216.p362GDg3017411-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {201104060216.p362GDg3017411-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-ID: TW35w4ZLZho05bSSQcAx44B8+Yxg+AlDf8pPaM-8WD2kLFzjXsTO2DdD1JqWI8Eg1d 6, 22 -- X-TOI-MSGID: 3ad2162a-dd00-4155-8a1f-9ea9ac7e039a ==============================End of - Headers==============================
Hello Ritchie, I do not have any experiences with 840 vacuum, but similar problem we had to solve on our old Balzers BAF 301. We were not able to get sufficient roughing vacuum and ODP did not start at all. The solution was really easy. We had to clean Pirani gauge because Pirani gauge with heavily contaminated measuring sensor gives wrong values of vacuum.
------------------------------------------------------- There is, in the old good Balzers manual, a procedure how to clean Balzers Pirani gauge TPR 010 (in short): - Dismount Pirani gauge and remove O-ring and porous filter. - Fill the measuring chamber with solvent (trichlor-ethylene, gasoline) close the opening with proper stopper and shake the gauge head thoroughly. - Remove the stopper and fill the gauge head completely with solvent. - Rest the gauge head for some longer time.
If this cleaning is not sufficient, there is a more rough method: - Evacuate the gauge head to the lowest possible vacuum. - Connect a foreign voltage of about 10 V to the pins of the measuring sensor and heat it for 10 seconds. --------------------------------------------------------
Please, the procedures above are valid only for this specific Balzers Pirani gauge TPR 010, but you may find something similar for your pirani gauge.
Good luck Oldrich
-- Oldrich Benada Institute of Microbiology ASCR, v.v.i. Vidensk8 1083 142 20 Prague 4
On Wednesday 06 of April 2011 04:13:50 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both arjay007-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: arjay007-at-gmail.com Name: Ritchie Sims } } Organization: The University of Auckland } } Title-Subject: [Filtered] some progress with 840 vacuum } } Message: Thanks to all who have replied. } } I realise that my initial posting may not have been completely clear: } } What I am having trouble with is getting the DPs down to a good roughing } vacuum before switching on the heaters ie all this is with the DPs cold. } In the past I have been able to get it down to 20uA (on the 100uA panel } meter that I have attached to the PG3 test points) with no problems, but } now it gets down only to 40uA, and although this is sufficient vacuum to } extinguish the PiG3 LED (and the AUTO mode would presumably take this as } good enough) I am somewhat un-nerved by the apparent change and too } suspicious to switch on the DP heaters. } Just now I have figured out, with the assistance of one of you, how to } defeat the 20-minute timer. It is the timer that shuts down the system } if the DPs have not warmed up within 20 minutes of startup, so defeating } it is just a matter of jumpering the warmup thermal switches on the two } DPs. This makes the HEATER LED turn off, and the timer (presumably IC } 2A, a type 51845 about which I can find no information) no longer } switches the system off. So I can leave the RP sucking on the cold DPs } overnight, see if the vacuum improves. } } And now I have had the courage to get the three system spaces down as } far as I can (still with the DPs cold) and open all the valves that are } usually open when it's in the "OK-to-use" state ie RP open to DPs, DPs } open to gun and column. The gun vacuum gauge reads about 0.03 mbar } (about 0.02 torr), so the backing pressure for the DPs must be at least } as low as this. } The question now is: Is 0.03mbar a good enough vacuum to switch on the } DP heaters without running the risk of charring the Santovac? } } all the best } Ritchie } } } } Message: Hi } I switched the 840 off while on vacation, and now I have vacuum } problems. With manual control of the valves, I can't get the RP to get } the DP pressure down to where it has been for years. I have a 100uA } meter connected to the PG3 test points down on the bottom RH corner of } the vacuum control board, usually this sits at about 10uA (where 70 or } 80 is about the level for the PiG3 warning LED to come on) but now I } can't get it below 45uA. The pressure is down low enough to switch PiG3 } LED off, but I really really don't want to risk cooking the DP Santovac. } Finding out what's going on is hampered by (a) the timer that switches } the whole thing off after 20minutes and (b) the fact that I don't really } know what backing pressure I am actually getting down to. } } Four questions: } } (a) How can I defeat the 20-minute switchoff timer so that I can at } least let the RP have a decent long suck on the (cold) DPs to see if it } will come right given enough time? } } (b) What does the backing pressure have to get down to in order to avoid } not cooking the Santovac? } } (c) Is it ok, after separately pumping the chamber, the gun, and the DPs } down to about 1 x 10-1 mbar, to then manually open the valves V2, V1A, } and V1B ie the valves as they are usually in the 'pumpdown' state, but } with the DPs cold? At least then I would know what backing pressure I } was getting down to, as I have 'proper' vacuum gauges on both the gun } and the chamber. Maybe it is OK to turn on the DP heaters, but I'm } reluctant to do so until I know what my backing pressure is. } } (d) Anyone got any suggestions or comments as to what might be going on? } } } Login Host: 130.216.59.110 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 21, 25 -- From } microscopylistserver-noreply-at-microscopy.com Tue Apr 5 21:11:06 2011 21, } 25 -- Received: from znl.com ([206.69.208.20]) } 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p362B6cO011233 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 } 21:11:06 -0500 21, 25 -- Received: from localhost (localhost [127.0.0.1]) } 21, 25 -- by znl.com (Postfix) with ESMTP id 6EF31373479 } 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 21:11:06 -0500 } (CDT) 21, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 21, 25 -- Received: from znl.com ([127.0.0.1]) } 21, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port 10024) 21, 25 -- with ESMTP id X1gy8PI8fL-J for } {microscopy-at-microscopy.com} ; 21, 25 -- Tue, 5 Apr 2011 21:11:05 -0500 } (CDT) } 21, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) 21, 25 -- by znl.com (Postfix) with ESMTPA id } 78EE537346E } 21, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Apr 2011 21:11:05 -0500 } (CDT) 21, 25 -- Message-ID: {4D9BCBB9.1040506-at-microscopy.com} } 21, 25 -- Date: Tue, 05 Apr 2011 21:11:05 -0500 } 21, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} 21, 25 -- Reply-To: } arjay007-at-gmail.com } 21, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 21, 25 -- MIME-Version: 1.0 } 21, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 21, 25 -- Subject: viaWWW:some progress with 840 vacuum } 21, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 21, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
I just read a fascinating article on Max Clara who is best known for his description of the bronchiolar epithelial cell commonly called a "Clara cell". The authors point out his work was aided by this "rather extensive and perfectly fixed material" from recently executed individuals and that he went on to be Chair of Anatomy at Leipzig University partly due to his prominence in the Nazi party. The authors argue that "club cell" might be a more fitting word to use in place of "Clara cell".
Eur Respir J. 2010 Oct;36(4):722-7. Winkelmann A, Noack T. The Clara cell: a "Third Reich eponym"?
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
One nice thing about having interested students is that they do not hesitate to ask questions that I have never thought much about.
The latest one came up while demonstrating the coaters in the lab. A student asked what to use to clean them and how often. I answered that I usually use a little water, maybe some detergent or a special solution that makes it easier to clean with water. How often was less clear, I have seen labs where bell jars etc are cleaned after each use and labs where it doesn't look like the glass has ever been cleaned.
What is your advice and experience?
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 11, 34 -- From jkrupp-at-deltacollege.edu Wed Apr 6 12:18:09 2011 11, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p36HI8ff002161 11, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 12:18:08 -0500 11, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 11, 34 -- by localhost (Email Security Appliance) with SMTP id 43491981505_D9CA04EB 11, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 17:18:06 +0000 (GMT) 11, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 11, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 17412981504_D9CA04EF 11, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 17:18:06 +0000 (GMT) 11, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 11, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 11, 34 -- ESMTP id LJ8QQ500.4PB for {Microscopy-at-microscopy.com} ; Wed, 6 11, 34 -- Apr 2011 10:18:05 -0700 11, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 11, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D907541204CF 11, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 10:18:05 -0700 (PDT) 11, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 11, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 11, 34 -- with ESMTP id 06CpKsiwkfJC for {Microscopy-at-microscopy.com} ; 11, 34 -- Wed, 6 Apr 2011 10:18:04 -0700 (PDT) 11, 34 -- Received: from [172.20.12.150] (unknown [172.20.12.150]) 11, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 9FAC1828703D 11, 34 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 10:18:04 -0700 (PDT) 11, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 11, 34 -- Content-Type: text/plain; charset=us-ascii 11, 34 -- Subject: To clean or not to clean bell jar 11, 34 -- Date: Wed, 6 Apr 2011 10:18:04 -0700 11, 34 -- Message-Id: {1C58F3D7-FDBD-4FF6-BAEB-69CE85328703-at-deltacollege.edu} 11, 34 -- To: Microscopy-at-microscopy.com 11, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 34 -- X-Mailer: Apple Mail (2.1084) 11, 34 -- Content-Transfer-Encoding: 8bit 11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p36HI8ff002161 ==============================End of - Headers==============================
I use Bon Ami and clean when it looks like it needs it. Nice and precise, yes? If I know I'm going to need a Wonderful Perfect Must Be High Resolution coat, I'll clean at least the day before and pump awhile to make sure any residual water vapor is gone.
Phil
} One nice thing about having interested students is that they do not } hesitate to ask questions that I have never thought much about. } } } The latest one came up while demonstrating the coaters in the lab. A } student asked what to use to clean them and how often. I answered } that I usually use a little water, maybe some detergent or a special } solution that makes it easier to clean with water. How often was } less clear, I have seen labs where bell jars etc are cleaned after } each use and labs where it doesn't look like the glass has ever been } cleaned. } } What is your advice and experience? } } } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 29 -- From oshel1pe-at-cmich.edu Wed Apr 6 12:29:09 2011 5, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p36HT8Ps018488 5, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 12:29:08 -0500 5, 29 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p36HT7Hm001555 5, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 13:29:08 -0400 5, 29 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 5, 29 -- Wed, 6 Apr 2011 13:29:08 -0400 5, 29 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 29 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Wed, 6 Apr 5, 29 -- 2011 13:29:08 -0400 5, 29 -- MIME-Version: 1.0 5, 29 -- Message-ID: {a06240809c9c252f5fad0-at-[141.209.160.249]} 5, 29 -- In-Reply-To: {201104061721.p36HLhMU007401-at-ns.microscopy.com} 5, 29 -- References: {201104061721.p36HLhMU007401-at-ns.microscopy.com} 5, 29 -- Date: Wed, 6 Apr 2011 13:29:05 -0400 5, 29 -- To: {Microscopy-at-microscopy.com} 5, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 29 -- Subject: Re: [Microscopy] To clean or not to clean bell jar 5, 29 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 29 -- X-Originating-IP: [141.209.160.249] 5, 29 -- X-OriginalArrivalTime: 06 Apr 2011 17:29:08.0256 (UTC) FILETIME=[22889A00:01CBF480] 5, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 29 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 5, 29 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 29 -- X-CanItPRO-Stream: default 5, 29 -- X-Canit-Stats-ID: 02Esht8w7 - d4092663b4cc - 20110406 5, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Bon Ami, eh? Have you had any problems with this in terms of getting carbon films to float off mica? I recall years ago trying various things to make bell jars easier to clean. Some really worked, but I often had problems getting films to float off after using them, even with long periods of time elapsed between cleaning and film preparation. Since then I've only used a tiny bit of levigated alumina, spread onto a coarse paper towel moistened with a bit of ethanol. But it takes a lot of scrubbing to get the bell jar and bottom of the evaporator clean. If Bon Ami does the job, and still lets films float off, I'd be "high vacuum happy".
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
On 06/04/2011 2:29 PM, oshel1pe-at-cmich.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Jon, } } I use Bon Ami and clean when it looks like it needs it. } Nice and precise, yes? } If I know I'm going to need a Wonderful Perfect Must Be High } Resolution coat, I'll clean at least the day before and pump awhile } to make sure any residual water vapor is gone. } } Phil } } } One nice thing about having interested students is that they do not } } hesitate to ask questions that I have never thought much about. } } } } } } The latest one came up while demonstrating the coaters in the lab. A } } student asked what to use to clean them and how often. I answered } } that I usually use a little water, maybe some detergent or a special } } solution that makes it easier to clean with water. How often was } } less clear, I have seen labs where bell jars etc are cleaned after } } each use and labs where it doesn't look like the glass has ever been } } cleaned. } } } } What is your advice and experience? } } } } } } } } Jonathan Krupp } } Delta College } } 5151Pacific Ave. } } Stockton, CA 95207 } } 209-954-5284 } } jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 21 -- From jehrman-at-mta.ca Wed Apr 6 12:39:23 2011 13, 21 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.4]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p36HdNUF002430 13, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 12:39:23 -0500 13, 21 -- Received: from host-22-194.mta.ca ([138.73.22.194]:64014) 13, 21 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 13, 21 -- (Exim 4.71) 13, 21 -- (envelope-from {jehrman-at-mta.ca} ) 13, 21 -- id 1Q7WhP-0003Rd-7o 13, 21 -- for Microscopy-at-microscopy.com; Wed, 06 Apr 2011 14:39:23 -0300 13, 21 -- Message-ID: {4D9CA548.3030300-at-mta.ca} 13, 21 -- Date: Wed, 06 Apr 2011 14:39:20 -0300 13, 21 -- From: James Ehrman {jehrman-at-mta.ca} 13, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 13, 21 -- MIME-Version: 1.0 13, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 13, 21 -- Subject: Re: [Microscopy] Re: To clean or not to clean bell jar 13, 21 -- References: {201104061729.p36HTSL9019086-at-ns.microscopy.com} 13, 21 -- In-Reply-To: {201104061729.p36HTSL9019086-at-ns.microscopy.com} 13, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 21 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
For sputter coater with rotary pump (which I use for magnifications not higher than 100k) I coat bell jar with a layer of soap. When jar is clean, I put some liquid soap (without moisturizer) on a wet piece of paper towel and wipe a jar. Then wipe it with a dry paper towel. For a few months (3-12) afterwards, I can easily remove a layer of metal just wiping it out with a piece of the dry paper towel; easy, fast and can be done in a few seconds.
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] } Sent: Wednesday, April 06, 2011 12:40 PM } To: Dusevich, Vladimir } Subject: [Microscopy] To clean or not to clean bell jar } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Phil, } } Bon Ami, eh? Have you had any problems with this in terms of getting } carbon films to float off mica? I recall years ago trying various } things } to make bell jars easier to clean. Some really worked, but I often had } problems getting films to float off after using them, even with long } periods of time elapsed between cleaning and film preparation. Since } then I've only used a tiny bit of levigated alumina, spread onto a } coarse paper towel moistened with a bit of ethanol. But it takes a lot } of scrubbing to get the bell jar and bottom of the evaporator clean. If } Bon Ami does the job, and still lets films float off, I'd be "high } vacuum happy". } } Jim } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } I doubt therefore I might be. } } } } } On 06/04/2011 2:29 PM, oshel1pe-at-cmich.edu wrote: } } } } } } --------------------------------------------------------------------- } ------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } --------------------------------------------------------------------- } ------- } } } } Jon, } } } } I use Bon Ami and clean when it looks like it needs it. } } Nice and precise, yes? } } If I know I'm going to need a Wonderful Perfect Must Be High } } Resolution coat, I'll clean at least the day before and pump awhile } } to make sure any residual water vapor is gone. } } } } Phil } } } } } One nice thing about having interested students is that they do not } } } hesitate to ask questions that I have never thought much about. } } } } } } } } } The latest one came up while demonstrating the coaters in the lab. A } } } student asked what to use to clean them and how often. I answered } } } that I usually use a little water, maybe some detergent or a special } } } solution that makes it easier to clean with water. How often was } } } less clear, I have seen labs where bell jars etc are cleaned after } } } each use and labs where it doesn't look like the glass has ever been } } } cleaned. } } } } } } What is your advice and experience? } } } } } } } } } } } } Jonathan Krupp } } } Delta College } } } 5151Pacific Ave. } } } Stockton, CA 95207 } } } 209-954-5284 } } } jkrupp-at-deltacollege.edu } } } } ==============================Original } Headers============================== } 13, 21 -- From jehrman-at-mta.ca Wed Apr 6 12:39:23 2011 } 13, 21 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.4]) } 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p36HdNUF002430 } 13, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 12:39:23 - } 0500 } 13, 21 -- Received: from host-22-194.mta.ca ([138.73.22.194]:64014) } 13, 21 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) } 13, 21 -- (Exim 4.71) } 13, 21 -- (envelope-from {jehrman-at-mta.ca} ) } 13, 21 -- id 1Q7WhP-0003Rd-7o } 13, 21 -- for Microscopy-at-microscopy.com; Wed, 06 Apr 2011 14:39:23 - } 0300 } 13, 21 -- Message-ID: {4D9CA548.3030300-at-mta.ca} } 13, 21 -- Date: Wed, 06 Apr 2011 14:39:20 -0300 } 13, 21 -- From: James Ehrman {jehrman-at-mta.ca} } 13, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; } rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 } 13, 21 -- MIME-Version: 1.0 } 13, 21 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} } 13, 21 -- Subject: Re: [Microscopy] Re: To clean or not to clean bell } jar } 13, 21 -- References: {201104061729.p36HTSL9019086-at-ns.microscopy.com} } 13, 21 -- In-Reply-To: {201104061729.p36HTSL9019086-at-ns.microscopy.com} } 13, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 21 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 29 -- From DusevichV-at-umkc.edu Wed Apr 6 13:18:08 2011 4, 29 -- Received: from mxtip01.um.umsystem.edu (mxtip01.um.umsystem.edu [209.106.229.20]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p36II8RM019361 4, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 13:18:08 -0500 4, 29 -- X-IronPort-Anti-Spam-Filtered: true 4, 29 -- X-IronPort-Anti-Spam-Result: AgUBAA6tnE2Dl3nN/2dsb2JhbACYMI1Md7pciG0CgxWCVQSFTo8M 4, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 4, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 06 Apr 2011 13:18:08 -0500 4, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.42]) by 4, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Wed, 6 Apr 2011 4, 29 -- 13:18:07 -0500 4, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 4, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 29 -- Date: Wed, 6 Apr 2011 13:18:07 -0500 4, 29 -- Subject: RE: [Microscopy] To clean or not to clean bell jar 4, 29 -- Thread-Topic: [Microscopy] To clean or not to clean bell jar 4, 29 -- Thread-Index: Acv0gcLah8MMrLinTI6jOz8xtvB0mwABR81A 4, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3AE1660C748-at-UM-EMAIL06.um.umsystem.edu} 4, 29 -- References: {201104061739.p36Hdl2i003199-at-ns.microscopy.com} 4, 29 -- In-Reply-To: {201104061739.p36Hdl2i003199-at-ns.microscopy.com} 4, 29 -- Accept-Language: en-US 4, 29 -- Content-Language: en-US 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- acceptlanguage: en-US 4, 29 -- Content-Type: text/plain; charset="us-ascii" 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p36II8RM019361 ==============================End of - Headers==============================
For high vacuum evaporators: A dirty bell jar slows down pumping and potentially could contaminate specimens if you are doing high resolution work. We shield our electrodes as much as possible, so non-essential coating does not end up on the entire bell jar. That way, we only have to clean the small area surrounding the electrodes.
We clean the entire bell jar maybe 4 times a year. We do coat the inside of the jar with Bell Bright, a detergent, that makes cleaning a simple task.
You could fashion shields from something like aluminum foil -- just be very careful not to contact any of the electrical feeds -- and then discard the foil.
For sputter coaters: We clean the glass containment vessel after each use. It's quick and easy, compared to the high vac system.
JB
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
On Wed, Apr 6, 2011 at 12:18 PM, {jkrupp-at-deltacollege.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } One nice thing about having interested students is that they do not hesitate to ask questions that I have never thought much about. } } } The latest one came up while demonstrating the coaters in the lab. A student asked what to use to clean them and how often. I answered that I usually use a little water, maybe some detergent or a special solution that makes it easier to clean with water. How often was less clear, I have seen labs where bell jars etc are cleaned after each use and labs where it doesn't look like the glass has ever been cleaned. } } What is your advice and experience? } } } } Jonathan Krupp } Delta College
==============================Original Headers============================== 10, 20 -- From bozzola-at-siu.edu Wed Apr 6 13:27:15 2011 10, 20 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p36IREQS003141 10, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 13:27:14 -0500 10, 20 -- Received: by iwg8 with SMTP id 8so2282308iwg.0 10, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 06 Apr 2011 11:27:13 -0700 (PDT) 10, 20 -- MIME-Version: 1.0 10, 20 -- Received: by 10.43.69.132 with SMTP id yc4mr2219068icb.221.1302114433807; Wed, 10, 20 -- 06 Apr 2011 11:27:13 -0700 (PDT) 10, 20 -- Received: by 10.43.64.209 with HTTP; Wed, 6 Apr 2011 11:27:13 -0700 (PDT) 10, 20 -- In-Reply-To: {201104061718.p36HIvPm003333-at-ns.microscopy.com} 10, 20 -- References: {201104061718.p36HIvPm003333-at-ns.microscopy.com} 10, 20 -- Date: Wed, 6 Apr 2011 13:27:13 -0500 10, 20 -- Message-ID: {BANLkTikxRud1ss+VfYR0z5=BVTNx_qgMCg-at-mail.gmail.com} 10, 20 -- Subject: Re: [Microscopy] To clean or not to clean bell jar 10, 20 -- From: John Bozzola {bozzola-at-siu.edu} 10, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 10, 20 -- Content-Type: text/plain; charset=UTF-8 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p36IREQS003141 ==============================End of - Headers==============================
The Southern California Society for Microscopy and Microanalysis (SCSMM) will hold its annual full day meeting on Saturday, April 16th at the South Coast Winery & Spa facilities in Temecula, CA.
For further details, contact info, and full agenda please visit the SCSMM web site: http://www.scsmm.org/
Registration deadline: 5 p.m. PST, Friday, April 8th, 2011
////////////////////////////////////////////////////////////////////////////////////////////////////////////// Krassimir Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside, CA 92521
phone 951 827 2998 fax 951 827 2489
==============================Original Headers============================== 10, 20 -- From bozhilov-at-ucr.edu Wed Apr 6 17:32:51 2011 10, 20 -- Received: from sentrell.ucr.edu (sentrell.ucr.edu [138.23.226.212]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p36MWpbX024358 10, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 6 Apr 2011 17:32:51 -0500 10, 20 -- Received: from cfamm2.ucr.edu (cfamm2.ucr.edu [138.23.103.15]) 10, 20 -- by sentrell.ucr.edu (MOS 3.10.5-GA) 10, 20 -- with ESMTP id RFP53425 (AUTH bozhilov) 10, 20 -- for {Microscopy-at-microscopy.com} ; 10, 20 -- Wed, 6 Apr 2011 15:32:50 -0700 (PDT) 10, 20 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu} 10, 20 -- Content-Type: text/plain; charset=us-ascii 10, 20 -- Subject: SCSMM meeting April 16th, 2011 10, 20 -- Date: Wed, 6 Apr 2011 15:32:50 -0700 10, 20 -- Message-Id: {284B4984-B4EF-4148-8D0C-0886A97A24E2-at-ucr.edu} 10, 20 -- To: Microscopy-at-microscopy.com 10, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 10, 20 -- X-Mailer: Apple Mail (2.1084) 10, 20 -- X-Junkmail-Status: score=0/50, host= 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p36MWpbX024358 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kvit-at-wisc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kvit-at-wisc.edu Name: Alexander Kvit
Organization: MSC, UW-Madison
Title-Subject: [Filtered] used holder for Philips CM200UT
Message: Hi, We are going to buy used double-tilt holder for Philips CM200UT microscope. Does somebody has it on a shelf? Do you know somebody who has it? We appreciate you for following this message to proper person.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dchristmass-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dchristmass-at-yahoo.com Name: David
Title-Subject: [Filtered] Microscope
Message: Can anyone assist with supplying a manual and brochures for a Chinese microscope marked Model L-201
A big thanks to all who offered their help for my IF problem. Because it may well help someone else in the future I will make a summary of the advices given to me. As a reminder I was trying to label a membrane-bound protein in IF and already tried 4% PFA +/- 0,01% triton X-100 without success.
1) Replace triton x-100 with saponin 2) Fix with methanol or methanol/acetone 3) Use antigen retrieval methods 4) Do no permeabilization (since its membrane-bound) 5) try to fix cell extracts with PFA for ELISA (since the antigen can be detected by ELISA with cell extracts) to see if PFA has an influence on the antigen recognition. 6) try other antibodies, consensus is to buy polyclonals.
I tried methanol/acetone fixation (-20°C) and I tried to directly label live cells at 37°C or at 4°C (to inhibit a possible turn-over of the antigen). I never got the faintest signal. I really think that the antibody is not able to recognize the antigen in these conditions. Since the labeling was just an additional method to reinforce previous results (with ELISA) I won't spend more time, efforts and money on it except perhaps buy applying antigen retrieval methods.
Final Score: Nature:1 Stephane:0 I lost this battle, but the struggle for knowledge goes on...
It seems that a primary source for objective lenses to fit 160mm tube length microscopes has dried-up. Can you help me out of a tight spot?
We have need for a matched set of four, 40x objectives for some Leica DMIL microscopes used for teaching. Our current 40x objectives are corrected for 1.1mm culture plates, while modern culture dishes are down to 0.63mm thickness. Does anyone know of any sources for such objectives? Our primary need is for GFP fluorescence detection, but phase contrast would be nice.
Thanks for any advice you can offer. I need an answer about this SOON! We have budget for lenses, but not for four new microscopes.
Regards, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan Molecular, Cellular, and Developmental Biology Ann Arbor, Michigan USA
Try to contact LOMO USA - they sell objectives for 160mm tube microscopes, but I am not sure either they offer objectives specifically corrected for culture dish thickness or not.
http://www.lomoamerica.com/OEMDataPage.htm
Good luck!
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 4/7/2011 9:44 AM, greggps-at-umich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It seems that a primary source for objective lenses to fit 160mm tube length microscopes has dried-up. Can you help me out of a tight spot? } } We have need for a matched set of four, 40x objectives for some Leica DMIL microscopes used for teaching. Our current 40x objectives are corrected for 1.1mm culture plates, while modern culture dishes are down to 0.63mm thickness. Does anyone know of any sources for such objectives? Our primary need is for GFP fluorescence detection, but phase contrast would be nice. } } Thanks for any advice you can offer. I need an answer about this SOON! We have budget for lenses, but not for four new microscopes. } } Regards, } ~Gregg } } Gregg Sobocinski } Microscope Imaging Specialist } University of Michigan } Molecular, Cellular, and Developmental Biology } Ann Arbor, Michigan } USA } } } } ==============================Original Headers============================== } 7, 27 -- From greggps-at-umich.edu Thu Apr 7 08:43:40 2011 } 7, 27 -- Received: from itcs-ehub-01.adsroot.itcs.umich.edu (itcs-ehub-01.adsroot.itcs.umich.edu [141.211.3.201]) } 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p37DheKC010814 } 7, 27 -- for {microscopy-at-microscopy.com} ; Thu, 7 Apr 2011 08:43:40 -0500 } 7, 27 -- Received: from ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu ([141.211.3.234]) by } 7, 27 -- itcs-ehub-01.adsroot.itcs.umich.edu ([141.211.3.201]) with mapi; Thu, 7 Apr } 7, 27 -- 2011 09:43:40 -0400 } 7, 27 -- From: "Sobocinski, Gregg" {greggps-at-umich.edu} } 7, 27 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} } 7, 27 -- Date: Thu, 7 Apr 2011 09:43:39 -0400 } 7, 27 -- Subject: Seeking vendor to supply objective lenses for 160mm tube length } 7, 27 -- microscopes. } 7, 27 -- Thread-Topic: Seeking vendor to supply objective lenses for 160mm tube } 7, 27 -- length microscopes. } 7, 27 -- Thread-Index: Acv1KYlnrIAyxaHYSBmw/UW6CRQtiQAAB2lg } 7, 27 -- Message-ID: {9F8ADD9ABC7F264E82EDDE4C10DA393425825C2840-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} } 7, 27 -- References: {9F8ADD9ABC7F264E82EDDE4C10DA393425825C283F-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} } 7, 27 -- In-Reply-To: {9F8ADD9ABC7F264E82EDDE4C10DA393425825C283F-at-ITCS-ECLS-1-VS3.adsroot.itcs.umich.edu} } 7, 27 -- Accept-Language: en-US } 7, 27 -- Content-Language: en-US } 7, 27 -- X-MS-Has-Attach: } 7, 27 -- X-MS-TNEF-Correlator: } 7, 27 -- acceptlanguage: en-US } 7, 27 -- Content-Type: text/plain; charset="us-ascii" } 7, 27 -- MIME-Version: 1.0 } 7, 27 -- Content-Transfer-Encoding: 8bit } 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p37DheKC010814 } ==============================End of - Headers============================== }
Dear Prof Mashore, It is possible that someone who has only completed a good microscopy course could fill the position, but there is a great deal of work and responsibility connected with it, and you will probably get only what you pay for. I would advertise for a Core Director, look for someone with a PhD in an appropriate field, and be willing to pay accordingly. You will want someone who will stay on the job for as long a time as possible, so make the salary, benefits, etc. as good as possible, and you might also think about hiring an assistant for the director if the work is too much for one person. Good luck. Yours, Bill
X-from: mmashore-at-vapop.ucsd.edu To: William.F.Tivol-at-aero.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear all,
I was hoping to get some expert advice about a job description. I have included the job description below. I would like receive feedback as to what a person who fulfills these responsibilities would be hired at (i.e. Core Director, Core Manager, Research Associate 1, etc.). I would also like to know what you would consider the highest level of education you think would be needed for this position. I realize the job description is quite long, but I appreciate any feedback.
Thank You.
Essential Duties and Responsibilities
1. Preparation of specimens such as paraffin, EM, hard tissue embedding and embedding frozen tissue in OCT compound. 2. EM, hard tissue, paraffin and frozen (cryosectioning) tissue sectioning. 3. Operate a Zeiss 10 Transmission Electron Microscope and Gatan 785 Digital Imaging system. 4. Operate a Zeiss LSM 510 and Pascal Confocal Microscope. 5. Operate a Zeiss Axio Observer, 21 Inverted Microscope with FRET, transmitted brightfield, DTC and Epi-fluorescence capabilities. 6. Requisitioning and Ordering supplies. 7. Maintains Equipment/supplies/Facilities: Maintains the laboratory facilities and equipment and controls supplies. Ensures that a sufficient supply of reagents, standards and controls are available at all times by ordering an adequate supply from commercial sources or preparing variety of specialized solutions or reagents. Using established procedures, calibrates, controls, and maintains a variety of instrumentation and laboratory equipment. When calibrations fall outside the acceptable limits, incumbent uses independent judgment in adjusting either the procedure or the mechanical device used in the determination to bring the procedures into good quality control. Plus general lab cleaning. 8. Customer Services: In accordance with Service procedures, answers the phone promptly in a courteous manner, identifying self and service. Similarly, outgoing calls are handled appropriately and politely. Valid complaints about failure to do so are not normally received by his/her supervisor. Shows positive evidence of a helpful attitude to other staff, visitors and clients through such actions as a consistent display of courtesy. Success in the area is measured by observation plus evaluation of any valid complaints and or commendations received during the rating period. Promotes attitude and procedures that foster both internal and external customer satisfaction. 9. Security: Incumbent is responsible for maintaining security of information in computerized systems in accordance with medial center policy: the Privacy Act; and other applicable laws, rules, regulations, and policies. 10. Safety: Inspect laboratory space for safe conditions and conformance to safety procedure. 11. Prepare core facilities invoices for jobs completed.
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p381exMJ009305 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 7 Apr 2011 20:40:59 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p381exPC009303; Thu, 7 Apr 2011 20:40:59 -0500
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball/NYU
Title-Subject: [Filtered] LED as alternative lightsource to halogen
Message: We have a Olympus IX70. To fit an external filter wheel, we had to put the 'scope up on bricks. This has bumped the top of the pillar up against the bottom of a utility shelf above the table which has led to the problem of not enough clearance for cooling the hot halogen bulb. I'm thinking of replacing this bulb with one to four LEDs (we don't need a lot of light for transmittance). Has anyone experience rewiring the stable power supply in the microscope body, perhaps by putting resistors in the wiring? Alternatively, by rewiring a shutter to turn on/off an LED instead of opening/closing a physical shutter?
(I thought of using a battery for power, but for overnight experiments we'd run though a lot of batteries unless someone wants to make an on/off serial port controlled switch for us.)
Thanks! ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both MLibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: MLibbee-at-gmail.com Name: Marissa Libbee
Organization: LBNL
Title-Subject: [Filtered] Black wax
Message: Can anyone tell me about black wax and where to get it? A microscopist suggested that this wax is cleanly removed with acetone, even more so than Crystalbond 509?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gul417-at-mail.usask.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gul417-at-mail.usask.ca Name: Guosheng Liu
Organization: U of Saskatchewan
Title-Subject: [Filtered] Philips 505 SEM, High Tension (kV) fluctuation problem Message: Hello,
There is an old SEM (Philips 505) in our lab that has worked fine until recently: the 3-digit numbers on the HT (kV) display fluctuate/jump in a range of plus/minus 3 at the setting of 20kV, more dramatically at lower settings. I am looking for your advice as to what likely causes this and where I should start to check? HT generator, vacuum or electronic board?
[Plus, this morning the HT was automatically shut-down during the normal operation (setting at 30kV--max in our SEM). Restart appears OK].
I am not aware of "black wax", but suspecting that maybe what you are looking for is "Black Nail Polish", sold by Ted Pella, Product Number 114-8
http://www.tedpella.com/section_html/section1.htm
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 4/7/2011 9:45 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both MLibbee-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: MLibbee-at-gmail.com Name: Marissa Libbee } } Organization: LBNL } } Title-Subject: [Filtered] Black wax } } Message: Can anyone tell me about black wax and where to get it? A } microscopist suggested that this wax is cleanly removed with acetone, } even more so than Crystalbond 509? } } Thanks, } Marissa } Login Host: 131.243.3.226 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Apr 7 20:41:26 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p381fQ26009436 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 7 Apr 2011 20:41:26 -0500 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 904BB375CD8 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 7 Apr 2011 20:41:26 -0500 (CDT) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id 1haOFkfXBnJv for {microscopy-at-microscopy.com} ; } 8, 25 -- Thu, 7 Apr 2011 20:41:26 -0500 (CDT) } 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 0DEA6375CCD } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 7 Apr 2011 20:41:26 -0500 (CDT) } 8, 25 -- Message-ID: {4D9E67C5.7060402-at-microscopy.com} } 8, 25 -- Date: Thu, 07 Apr 2011 20:41:25 -0500 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: MLibbee-at-gmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:Black wax } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
Whilst the fluctuating numbers may indicate a problem I would be more interested in what is happening to the image? With such a fluctuation I would expect the focus to change considerably during observation? Focus an image at a around 10,000 to 20,000X and record the image; does the focus change?
Answering that question as YES then you are rightly worried about the high voltage generating system. If the answer is NO carry on and take no notice of a flickering indicator.
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Title-Subject: [Filtered] Philips 505 SEM, High Tension (kV) fluctuation problem Message: Hello,
There is an old SEM (Philips 505) in our lab that has worked fine until recently: the 3-digit numbers on the HT (kV) display fluctuate/jump in a range of plus/minus 3 at the setting of 20kV, more dramatically at lower settings. I am looking for your advice as to what likely causes this and where I should start to check? HT generator, vacuum or electronic board?
[Plus, this morning the HT was automatically shut-down during the normal operation (setting at 30kV--max in our SEM). Restart appears OK].
In 1976 I started in a lab that had some sticks of black wax in the cabinet. I never used it but thought that it was possibly used to seal vacuum leaks. I remember that they were very hard and not what I would consider wax like. It had an orange label on it and I think it may have been Apizion or something similar to that printed on it. I think it may have come from Thomas or VWR as they supplied a lot of the things that I inherited there.
If they have not contacted you in response to your posting, I'd suggest you phone or email one of the EM product suppliers like SPI, Electron Microscopy Sciences, Ted Pella to name a few for they all have a vast knowledge of products that are currently available.
You might wish to post what you want to accomplish by using the black wax so we can make suggestions or go back to the person who suggested it to you and ask for the supplier information that he may have.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
We buy black wax from SPI supplies (http://www.2spi.com) and it is indeed made by Apiezon http://www.2spi.com/catalog/vac/apiezon/
We find it more robust in certain applications than the regular crystal bond clear wax (resistant to acids for instance).
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
-------- Title-Subject: [Filtered] Black wax
Message: Can anyone tell me about black wax and where to get it? A microscopist suggested that this wax is cleanly removed with acetone, even more so than Crystalbond 509?
CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation.
South Bay Technology offers black wax which is designed for use as a masking material in jet polishing applications. As it is chemically inert, it is resistant to etching solutions such as hydrofluoric acid, nitric acid, perchloric acid and acetic acid. While it is resistant to many acids, it can be easily removed with hydrocarbon or chlorinated solvents. Black wax softens at 85oC and flows at 100oC.
By far, the most common of our wax materials used in sample preparation is our acetone soluble QuickStick 135. However, we do have several wax products which enable you to select the best mix of solubility, melting point, hardness etc. for your application. We do send out sample packages of the various wax products so we'd be happy to send you one so you can try them out. Be sure to specify that you want to try the black wax as well as I don't think it is included with the normal sample pack.
I hope that helps.
Best regards-
David
David Henriks
South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673 USA
tel: +1-949-492-2600 fax: +1-949-492-1499
email: HenriksSBT-at-cox.net
www.southbaytech.com
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, April 07, 2011 6:52 PM To: Henriks-at-cox.net
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both MLibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: MLibbee-at-gmail.com Name: Marissa Libbee
Organization: LBNL
Title-Subject: [Filtered] Black wax
Message: Can anyone tell me about black wax and where to get it? A microscopist suggested that this wax is cleanly removed with acetone, even more so than Crystalbond 509?
Hoping someone may have solution to our Gatan 794 controller problem. We can not acquire a proper gain reference image - it returns a banded image.
Our unit is in Warrendale for repair and we will be getting it back shortly. However, the controller is now officially out of production and not for sale. This kind of makes me nervous, because it puts down our JEOL 2010 which we use in production.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both frohlich-at-uthscsa.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: frohlich-at-uthscsa.edu Name: Victoria Frohlich
Organization: UTHSCSA
Title-Subject: [Filtered] Assistant or Associate Director Position Available
Message: Assistant or Associate Professor of Research
The University of Texas Health Science Center at San Antonio is seeking applications for a faculty position at the rank of Assistant or Associate Professor of Research in the Department of Cellular & Structural Biology, who will serve as the Assistant / Associate Director of the Core Optical Imaging Facility. The Core Optical Imaging Facility houses state-of-the-art equipment and includes confocal laser scanning microscopy, multiphoton microscopy, total internal reflection microscopy, super-optical resolution microscopy as well as widefield and dissecting fluorescent microscopes. This position will direct, coordinate, supervise, and participate in all aspects of the daily operation of the imaging core. Responsibilities include management of technical personnel, oversight of daily core facility operations, development, operation and maintenance of specialized optical imaging equipment resident in the core facility, as well as develop budgets and oversee monthly billing. The Assistant/Associate Director will be involved in training clients to use instrumentation resident in the imaging core as well as consulting with clients on the design and specimen preparation techniques for experiments involving imaging core activities. In addition, the Assistant/Associate Director will assist in writing grants directly impacting core activities or in writing technical portions of papers and be responsible for collecting and compiling of data on core usage for the purpose of reporting on core activities to university and/or government agencies. A PhD in an appropriate scientific field with 3-5 post-degree years of relevant and productive work experience is required. Demonstrated excellence in the management of a light microscopy shared resource and in continuing education in advanced light microscopy techniques are preferred.
To apply, please forward a curriculum vitae electronically to facsearchcsb-at-uthscsa.edu. Alternatively, applications may be mailed to: Faculty Search Committee; Department of Cellular & Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900. Initial review of applications will begin immediately and will remain open until the position is filled. Salary and rank are commensurate with experience. All faculty appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Employment Opportunity/Affirmative Action Employer.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both aleb-at-nhm.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: aleb-at-nhm.ac.uk Name: Alex Ball
Organization: The Natural history Museum, London
Title-Subject: [Filtered] LED as an alternative lightsource to halogen
Message: Hi Michael,
We've recently replaced the mercury vapour lamp on our Leica confocal microscope with an LED system manufactured by CoolLED. In our application we've fitted fluorescence LEDs, but I'm sure they could fit white light modules as well. The system was easy to install and set up and of course, as it says - cool! I'd check them out and see if they offer anything that could solve your problem. http://www.coolled.com/Life-Sciences-Analytical/
Regards,
Alex Ball
Dr Alex Ball Electron Microscope Unit Manager The Natural History Museum London SW7 5BD
Title-Subject: [Filtered] LED as alternative lightsource to halogen
Message: We have a Olympus IX70. To fit an external filter wheel, we had to put the 'scope up on bricks. This has bumped the top of the pillar up against the bottom of a utility shelf above the table which has led to the problem of not enough clearance for cooling the hot halogen bulb. I'm thinking of replacing this bulb with one to four LEDs (we don't need a lot of light for transmittance). Has anyone experience rewiring the stable power supply in the microscope body, perhaps by putting resistors in the wiring? Alternatively, by rewiring a shutter to turn on/off an LED instead of opening/closing a physical shutter?
(I thought of using a battery for power, but for overnight experiments we'd run though a lot of batteries unless someone wants to make an on/off serial port controlled switch for us.)
Thanks! ________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brossetti-at-mbl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: brossetti-at-mbl.edu Name: Blair Rossetti
Organization: NESM
Title-Subject: [Filtered] NESM SPRING SYMPOSIUM WOODS HOLE - MAY 6, 2011
Message: NESM SPRING SYMPOSIUM WOODS HOLE, MA - MAY 6, 2011
Hi Microscopy ListServer-ites,
SAVE THE DATE: May 6th For the upcoming New England Society for Microscopy's 28th Annual Woods Hole, MA Symposium.
May 6 -- NESM Symposium, 10:00AM - 5:00PM
To register by April 27th, please send your name and affiliation to brossetti-at-mbl.edu
Registration Costs (including lunch and an afternoon coffee break): $65 NESM Members $90 Nonmembers (includes 2011-year membership) $30 Students $50 Retirees $50 Guests
More details (speakers/titles) to follow in the upcoming April Newsletter and at the NESM website: http://nesm.cims.harvard.edu/
Student Poster Session: Students who present posters at a NESM meeting will be automatically entered to win a prize. Prizes will be announced and awarded on the day of the NESM meeting.
Bring A Colleague: NESM members who bring two new members to join during 2011, will receive free membership for 2012!!!
Thank you for your attention and consideration; and we look forward to seeing you in Woods Hole.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both istvan.abraham-at-otago.ac.nz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: istvan.abraham-at-otago.ac.nz Name: Dr Istvan Abraham
Organization: University of Otago, New Zealand
Title-Subject: [Filtered] TIRF-need help on MetaMorph (Hamamatsu C9100-13 EM CCD, Olympus BX-81)
------------------------------------------------------------------------ NOTE TO READERS: I HAVE REMOVED REFERENCES TO INDIVIDUALS AND TEXT IN THE BODY OF THIS MESSAGE WHICH I DEEMED INCONSISTENT WITH LISTSERVER RULES.
We are using an Olympus TIRF system mounted on BX-81 microscope with a new Hamamatsu C9100-13 EM CCD camera for single molecule detection. We drive the microscope, lasers and camera with help MetaMorph software under Windows XP. We are streaming all data directly to hard drive during acquisition in “overlapped mode” to use the “parallel acquisition and read out” feature of the camera. Unfortunately we cannot achieve the maximum acq. time (32 ms at binning 1) because the MetaMorph is freezing at around 60 ms or shorter acq.. We observed this phenomenon after 700-8000 frames. The number of acquired frames are randomly varied and did not depend on the actual settings or experimental conditions. Interestingly, we also received error messages sometimes saying that the software cannot handle the images because of “insufficient memory”. However the 4 GB RAM does not reach the 20% of its capacity during acquisition and streaming. Furthermore, we have 1 TB SAS HDs in RAID 10 configuration with powerful frame grabber in our server PC (designed to the TIRF measurement) that enables us huge writing speed and big space for our images (513 KB each).
Our situation is difficult because the New Zealand distributor, Bio-Strategy (XXXXXXXXXX-names omitted), does not seem to have an expert on board and they also (XXXXXXXX - Word changed) (declined) to give any onsite support for the MetaMorph. We received some support from US (Molecular Devices (MD)) via Webinar sessions but they were mainly ineffective in solving this “freezing” issue. Worth noting, we also reported to MD that MetaMorph did not to handle the parallel read out and acq. feature of Hamamatsu C9100-13 EM CCD. Based on this observation the MD introduced the “overlapped mode” in the MetaMoprh software to use the parallel readout feature of this camera.
As it looks now, however, MD lost its interest in working further on MetaMorph to make it really compatible with Hamamatsu C9100-13 EM CCD camera. However we would like to achieve the 32 ms acq. time with this camera because it is essential for our single molecule detection experiments.
We highly appreciate any idea or help on this subject.
Many thanks Istvan
Istvan Abraham MD, PhD Centre for Neuroendocrinology & Dept. of Physiology University of Otago, Lindo Ferguson Building, 270 Great King street, PoBOX 913 9054, Dunedin, New Zealand T:64 3 479 4862 F:64 3 479 7323 http://phsl.otago.ac.nz/abraham/index.html
Had a similar problem with the Olympus-SIS digital camera. One problem might the operating system, have you upgraded to Windows 7? In my case it was the firewire card that went bad, replaced it and all is fine now.
Ed
-----Original Message----- X-from: Bryan.Tracy-at-spansion.com [mailto:Bryan.Tracy-at-spansion.com] Sent: Friday, April 08, 2011 7:03 PM To: Calomeni, Edward
Hi Friends
Hoping someone may have solution to our Gatan 794 controller problem. We can not acquire a proper gain reference image - it returns a banded image.
Our unit is in Warrendale for repair and we will be getting it back shortly. However, the controller is now officially out of production and not for sale. This kind of makes me nervous, because it puts down our JEOL 2010 which we use in production.
We have a 63x Plan Apo Zeiss objective that has been on an inverted microscope for years. I noticed the other day that the barrel no longer moves and I am pretty sure it was at one time spring loaded. Presumably crud has gotten inside and frozen the mechanism. I tried removing the small set screw on the side of the barrel to see if I could remove the casing and get at the mechanism and hopefully free it but the casing did not come off easily. I presume that I should not add lubricant/penetrant between the inner and outer barrel to free it. I could use my strap wrench to try to free the casing. Any suggestions?
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Mon Apr 11 09:22:00 2011 5, 19 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BEM0co016909 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 09:22:00 -0500 5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) 5, 19 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 766012D064 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 10:22:00 -0400 (EDT) 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=us-ascii 5, 19 -- Subject: Stuck Zeiss objective 5, 19 -- Date: Mon, 11 Apr 2011 10:22:00 -0400 5, 19 -- Message-Id: {298204B0-70CE-404D-825F-06A9C0A338CD-at-uconn.edu} 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 19 -- X-Mailer: Apple Mail (2.1082) 5, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 5, 19 -- X-Virus-Status: Clean 5, 19 -- Content-Transfer-Encoding: 8bit 5, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3BEM0co016909 ==============================End of - Headers==============================
I still have a number of Gatan DuoMill spare parts in my lab, which I am about to throw out. Several specimen tables, O-rings, flanges, HV connectors etc.
If anyone is interested in it, I would just put (throw, to be precisely) everything in a box and send it to whoever wants it. I would not pay for special air freight, customs fees etc.
Please let me know within one week if you are interested - Joe Zweck
==============================Original Headers============================== 6, 23 -- From Josef.Zweck-at-physik.uni-regensburg.de Mon Apr 11 09:37:32 2011 6, 23 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BEbWK7000837 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 09:37:32 -0500 6, 23 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 6, 23 -- by localhost (Postfix) with SMTP id B642337DB 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 16:37:26 +0200 (CEST) 6, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 6, 23 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id A766437CA 6, 23 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 16:37:26 +0200 (CEST) 6, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 6, 23 -- with Novell_GroupWise; Mon, 11 Apr 2011 16:37:27 +0200 6, 23 -- Message-Id: {4DA32E450200004000012041-at-gwsmtp1.uni-regensburg.de} 6, 23 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 6, 23 -- Date: Mon, 11 Apr 2011 16:37:25 +0200 6, 23 -- From: "Josef Zweck" {Josef.Zweck-at-physik.uni-regensburg.de} 6, 23 -- To: "Listserver Microscopy" {microscopy-at-microscopy.com} 6, 23 -- Subject: Gatan DuoMill parts 6, 23 -- Mime-Version: 1.0 6, 23 -- Content-Type: text/plain; charset=US-ASCII 6, 23 -- Content-Disposition: inline 6, 23 -- Content-Transfer-Encoding: 8bit 6, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3BEbWK7000837 ==============================End of - Headers==============================
On Apr 11, 2011, at 10:46 AM, Mark, William wrote:
} David, } } You might try xylene (just a little bit) or Goofoff and a strap wrench. } I believe that this objective has a collar for coverslip thickness, no? } } Bill } } -----Original Message----- } From: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] } Sent: Monday, April 11, 2011 10:30 AM } To: Mark, William } Subject: [Microscopy] Stuck Zeiss objective } } } } } ------------------------------------------------------------------------ } ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ } ---- } } We have a 63x Plan Apo Zeiss objective that has been on an inverted } microscope for years. I noticed the other day that the barrel no longer } moves and I am pretty sure it was at one time spring loaded. Presumably } crud has gotten inside and frozen the mechanism. I tried removing the } small set screw on the side of the barrel to see if I could remove the } casing and get at the mechanism and hopefully free it but the casing did } not come off easily. I presume that I should not add } lubricant/penetrant between the inner and outer barrel to free it. I } could use my strap wrench to try to free the casing. Any suggestions? } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } } } } } ==============================Original } Headers============================== } 5, 19 -- From david.knecht-at-uconn.edu Mon Apr 11 09:22:00 2011 } 5, 19 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu } [137.99.25.234]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id p3BEM0co016909 } 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 } 09:22:00 -0500 } 5, 19 -- Received: from d46h164.public.uconn.edu } (d46h164.public.uconn.edu [137.99.46.164]) } 5, 19 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id } 766012D064 } 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 } 10:22:00 -0400 (EDT) } 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} } 5, 19 -- Content-Type: text/plain; charset=us-ascii } 5, 19 -- Subject: Stuck Zeiss objective } 5, 19 -- Date: Mon, 11 Apr 2011 10:22:00 -0400 } 5, 19 -- Message-Id: {298204B0-70CE-404D-825F-06A9C0A338CD-at-uconn.edu} } 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 5, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) } 5, 19 -- X-Mailer: Apple Mail (2.1082) } 5, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 5, 19 -- X-Virus-Status: Clean } 5, 19 -- Content-Transfer-Encoding: 8bit } 5, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3BEM0co016909 } ==============================End of - } Headers==============================
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
I was rereading the section of MicroscopyU on Phase contrast microscopy, and one aspect of it confused me (again). I usually explain the contrast as a measure of refractive index differences between parts of the cell and the surrounding medium. The refractive index differences lead to differences in optical path length which leads to waves being out of phase. But changes in refractive index also deviates the light. MicrosopyU calls the deviated wave in phase the diffracted wave, but then proceeds to lay out the math in terms of refractive index. So what is the correct way to say this? Is it that the differences in the refractive index of the medium through which the diffracted light passes that changes the optical path length? Does deviation by refraction play any role? Thanks- Dave
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 5, 19 -- From david.knecht-at-uconn.edu Mon Apr 11 10:40:19 2011 5, 19 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BFeJKh002049 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 10:40:19 -0500 5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) 5, 19 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 2961C2D088 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 11:40:19 -0400 (EDT) 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} 5, 19 -- Content-Type: text/plain; charset=us-ascii 5, 19 -- Subject: Phase contrast again- diffraction vs. refraction 5, 19 -- Date: Mon, 11 Apr 2011 11:40:18 -0400 5, 19 -- Message-Id: {DCE504F3-83F5-4AB6-B785-ACF6144B45F1-at-uconn.edu} 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 5, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 19 -- X-Mailer: Apple Mail (2.1082) 5, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 5, 19 -- X-Virus-Status: Clean 5, 19 -- Content-Transfer-Encoding: 8bit 5, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3BFeJKh002049 ==============================End of - Headers==============================
I'm not sure about the official or definitive answer but diffraction from an aperture edge would happen in the absence of a refractive medium (such as in a vacuum). Diffraction is where a secondary wave (Huygens' secondary source) is created by scattering rather than a modification to a primary wave , which is what I think is called refraction. Diffracted waves have a stepwise difference in phase from the primary wave - often 90-degrees advanced.
If the refraction of the cell and the medium differ (because of differences in refractive index and possibly thickness) then there will be optical path differences that translate directly into phase contrast, resulting from the recombination of the differently refracted waves.
Diffraction is better known from the X-ray and electron study of crystals; and in the electron microscope combining diffracted with non-diffracted beams can deliver atomic resolution (lattice) images.
Good luck with your instruction, but I am not familiar with MicroscopyU.
Rob Keyse Lehigh University EM Facility
On 4/11/2011 11:49 AM, david.knecht-at-uconn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I was rereading the section of MicroscopyU on Phase contrast microscopy, and one aspect of it confused me (again). I usually explain the contrast as a measure of refractive index differences between parts of the cell and the surrounding medium. The refractive index differences lead to differences in optical path length which leads to waves being out of phase. But changes in refractive index also deviates the light. MicrosopyU calls the deviated wave in phase the diffracted wave, but then proceeds to lay out the math in terms of refractive index. So what is the correct way to say this? Is it that the differences in the refractive index of the medium through which the diffracted light passes that changes the optical path length? Does deviation by refraction play any role? Thanks- Dave } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } }
F.Zernike, Z. Tech. Phys., 16 (1935), 848; Physica, 9(1942), 686, 97F4. (refs from Born and Wolfe, 7th ed, 'Principles of Optics')
F. Zernike - Nobel Prize Talk: http://nobelprize.org/nobel_prizes/physics/laureates/1953/zernike-lecture.html
Hope this helps,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl ========================================================================= Abraham Lincoln was perhaps the greatest orator the United States has known since the days of the Founders. Read what he said, then, think and learn. http://showcase.netins.net/web/creative/lincoln/speeches/lyceum.htm And, what's a lyceum? http://dig.lib.niu.edu/ISHS/ishs-1990spring/ishs-1990spring45.pdf ========================================================================= History is Cyclic. Only the ism's, nouns or adjectives change, AND......, When the objective classroom turns to agenda, history repeats! ========================================================================= "Pan-Germanism was an achievement of intellectuals and writers. The professors of history, law, economics, political science, geography, and philosophy were its most uncompromising advocates. They converted the students of the universities to their ideas. Very soon the graduates made more converts. As teachers in the field of higher education (in the famous German Gymnasium and educational institutions of the same rank), as lawyers, judges, civil servants, and diplomats they had ample opportunity to serve their cause."
""Soap capital" desires more cleanliness, "building capital" a greater demand for homes, "publishing capital" more and better education, and "armaments capital" bigger armaments. The shortrun interests of every branch of business encourage such attitudes. In the long run, however, increased demand results in an inflow of more capital into the booming branch, and the competition of the new enterprises cuts down the profits." Ludwig von Mises (http://www.mises.org/etexts/mises/og/chap6.asp) ========================================================================= Thus, the wise state, looking for the best result, will support research in those endeavors that promise improvements in the 'general welfare'. The 'wise state' will know that one cannot order up the solution to any problem by treating the process of innovation as little more than magic. The 'unwise state' will create chaos. (FCM, 2011) =========================================================================
Dear David, The two descriptions--refractive index and diffraction--are equivalent, so it does not matter which description you use. Some people find the refractive index easier to understand, and others are more comfortable with the diffraction picture. Regarding your question about the role of deviation, remember that a lens transfers the light (or electrons in the EM) from one point in the specimen to one point in the image, regardless of the direction that the light leaves the specimen (as long as it is within the solid angle of the lens, of course). The total path length will depend on the angle of deviation, and this will affect the phase. In TEM the angles of deviation are very small, so the phase differences are correspondingly small, and the small-angle approximation is used, and the differences are ignored. In LM with a lens with high NA, some of the angles can be large, so some of the light may be sufficiently out of phase that the contrast is lowered. Yours, Bill
X-from: david.knecht-at-uconn.edu To: William.F.Tivol-at-aero.org
I was rereading the section of MicroscopyU on Phase contrast microscopy, and one aspect of it confused me (again). I usually explain the contrast as a measure of refractive index differences between parts of the cell and the surrounding medium. The refractive index differences lead to differences in optical path length which leads to waves being out of phase. But changes in refractive index also deviates the light. MicrosopyU calls the deviated wave in phase the diffracted wave, but then proceeds to lay out the math in terms of refractive index. So what is the correct way to say this? Is it that the differences in the refractive index of the medium through which the diffracted light passes that changes the optical path length? Does deviation by refraction play any role? Thanks- Dave
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BJQU1V005700 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 11 Apr 2011 14:26:30 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3BJQU4h005698; Mon, 11 Apr 2011 14:26:30 -0500
My question might be a bit basic for this list, but since we're on the subject. I've always had difficulty understanding how phase-contrast operates in terms of creating the two phases of light. (That way the two work to create contrast through interference I get.) But how does one light path for the background, and another sample/refracted light path off by 1/4 wavelength get created? I've never figured out how the condenser ring and the objective ring interact to do this.
==============================Original Headers============================== 1, 21 -- From germpore-at-sonic.net Mon Apr 11 14:26:29 2011 1, 21 -- Received: from qmta06.emeryville.ca.mail.comcast.net (qmta06.emeryville.ca.mail.comcast.net [76.96.30.56]) 1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BJQTam005695 1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 14:26:29 -0500 1, 21 -- Received: from omta16.emeryville.ca.mail.comcast.net ([76.96.30.72]) 1, 21 -- by qmta06.emeryville.ca.mail.comcast.net with comcast 1, 21 -- id WJtK1g0061ZMdJ4A6KSUd3; Mon, 11 Apr 2011 19:26:28 +0000 1, 21 -- Received: from [192.168.2.15] ([24.5.115.217]) 1, 21 -- by omta16.emeryville.ca.mail.comcast.net with comcast 1, 21 -- id WKST1g00B4hVnAG8cKSTkq; Mon, 11 Apr 2011 19:26:27 +0000 1, 21 -- Message-Id: {FC0A8AEA-3AEF-4FEC-8FD3-0C6EA969E44C-at-sonic.net} 1, 21 -- From: Peter Werner {germpore-at-sonic.net} 1, 21 -- To: Microscopy-at-microscopy.com 1, 21 -- In-Reply-To: {201104111856.p3BIuh0b003491-at-ns.microscopy.com} 1, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 1, 21 -- Content-Transfer-Encoding: 7bit 1, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 1, 21 -- Subject: Re: Phase contrast again- diffraction vs. refraction 1, 21 -- Date: Mon, 11 Apr 2011 12:26:27 -0700 1, 21 -- References: {201104111856.p3BIuh0b003491-at-ns.microscopy.com} 1, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
We have a client who is a cardiac surgeon using SEM to look at surgical burrs. These are diamond-encrusted rotary tips that are threaded up into the heart to drill out plaque. The current question is how much tissue is sticking to the burrs and preventing the diamonds from cutting. He originally wanted to see how much calcified plaque was present, and this was easy, using EDS to detect the Ca, color it in a map, and then do image analysis to see how much area was covered. I can't figure out how to look at non-calcified plaque. It's carbon, as are the diamonds. The adhesive is Ni and P, on a stainless steel base. Can anyone think of something I can "stain" the tissue with to be able to detect it with the EDS (this being easier and faster than identifying and hand selecting with ImageJ)?
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Mon Apr 11 16:41:10 2011 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BLf9B6025426 4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 16:41:10 -0500 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p3BLf7ca016979 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 11:41:07 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p3BLf6pk016975 4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 11:41:06 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Mon, 11 Apr 2011 11:41:06 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Detecting tissue on surgical burr with SEM/EDS 4, 20 -- Message-ID: {Pine.GSO.4.64.1104111133320.14980-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Hi Tina, I don't really know, but might Os or some of the heavy TEM stains give you the differentiation you need?
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: tina-at-pbrc.hawaii.edu [mailto:tina-at-pbrc.hawaii.edu] Sent: Monday, April 11, 2011 5:44 PM To: kenconverse-at-qualityimages.biz
Hi, All-
We have a client who is a cardiac surgeon using SEM to look at surgical burrs. These are diamond-encrusted rotary tips that are threaded up into the heart to drill out plaque. The current question is how much tissue is sticking to the burrs and preventing the diamonds from cutting. He originally wanted to see how much calcified plaque was present, and this was easy, using EDS to detect the Ca, color it in a map, and then do image analysis to see how much area was covered. I can't figure out how to look at non-calcified plaque. It's carbon, as are the diamonds. The adhesive is Ni and P, on a stainless steel base. Can anyone think of something I can "stain" the tissue with to be able to detect it with the EDS (this being easier and faster than identifying and hand selecting with ImageJ)?
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Mon Apr 11 16:41:10 2011 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BLf9B6025426 4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 16:41:10 -0500 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p3BLf7ca016979 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 11:41:07 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p3BLf6pk016975 4, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 11:41:06 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Mon, 11 Apr 2011 11:41:06 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: Detecting tissue on surgical burr with SEM/EDS 4, 20 -- Message-ID: {Pine.GSO.4.64.1104111133320.14980-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
==============================Original Headers============================== 15, 28 -- From kenconverse-at-qualityimages.biz Mon Apr 11 16:59:19 2011 15, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3BLxHR4009454 15, 28 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 16:59:18 -0500 15, 28 -- X-Authority-Analysis: v=1.1 cv=2pE2Kh9Ye2ywHyyFZnC5ZQ1FvuPrdOtuPO5uN4ysVDU= c=1 sm=0 a=PEKvsdFYFfAA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=yY7Kid_uAAAA:8 a=6Qq2YxHfqsbsfXkPyYMA:9 a=3V9DD1nEtkVp7UzWqmsA:7 a=CjuIK1q_8ugA:10 a=lrwkLBK0R5QA:10 a=5JUlSvKOtR0A:10 a=aPbrbJDOxB0A:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 15, 28 -- X-Cloudmark-Score: 0 15, 28 -- X-Originating-IP: 72.224.149.57 15, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1941] helo=Ken) 15, 28 -- by cdptpa-oedge03.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 15, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 15, 28 -- id B6/69-05159-4B973AD4; Mon, 11 Apr 2011 21:59:17 +0000 15, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 15, 28 -- To: {tina-at-pbrc.hawaii.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 15, 28 -- Subject: RE: [Microscopy] Detecting tissue on surgical burr with SEM/EDS 15, 28 -- Date: Mon, 11 Apr 2011 17:59:06 -0400 15, 28 -- Message-ID: {00EDE41B7AFF434681877828CE8FEF7F-at-Ken} 15, 28 -- MIME-Version: 1.0 15, 28 -- Content-Type: text/plain; 15, 28 -- charset="US-ASCII" 15, 28 -- X-Priority: 3 (Normal) 15, 28 -- X-MSMail-Priority: Normal 15, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 15, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 15, 28 -- In-Reply-To: {201104112144.p3BLiQmp028934-at-ns.microscopy.com} 15, 28 -- Thread-Index: Acv4kaMRqsACBdK9QhK/3ItxjVmgQAAAZqJg 15, 28 -- Importance: Normal 15, 28 -- Content-Transfer-Encoding: 8bit 15, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3BLxHR4009454 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dhorne-at-interchange.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Emitech transver valve/spare parts
Message: Does anyone have an old Emitech K1250 sitting around, mothballed and not likely to go back into service? We might be looking for another transfer device. Feel free to contact me offline.
We recently discovered (yesterday), that our CCD surface is contaminated with fluid and solid particles, the problem is that is affecting image quality.
Any of You can recommend a cleaning procedure for this surface?
Laci
-- Jakab-Farkas Laszlo Labortechnikus
SAPIENTIA - ERDÉLYI MAGYAR TUDOMÁNYEGYETEM MÜSZAKI ÉS HUMÁNTUDOMÁNYOK KAR MAROSVÁSÁRHELY Campus: Segesvári út 1C., Marosvásárhely/Koronka (a város határában) Tel.: +40-(0)265-206210,+40-(0)265-208170, fax: 0265-206211 Mobil.: +40-(0)745-873844 E-mail: jflaci-at-ms.sapientia.ro Postacím: 540485 Tîrgu Mures,op 9 cp4.
Those discussions were about removing objectives that were stuck in the turret. That was not a problem here. What is stuck is the spring loaded barrel of the objective. I am trying to find out if putting some penetrant on the barrel will have any chance of getting to the optics. Dave
On Apr 12, 2011, at 3:37 AM, Stephane Nizet wrote:
} Hi David! } } This subject has already been discussed on the list in the past. If I remember } well some suggestions involved the use of oil while others advised against it. } Using the wrench was an option, provided you use a cloth to avoid scraping the } surface and help distribute the pressure on the cylinder. } There were also suggestions to use prevention. I would suggest you search the } archives of the list. } } Best regards, } } Stephane } } } } ----- Original Message ---- } From: "david.knecht-at-uconn.edu" {david.knecht-at-uconn.edu} } To: nizets2-at-yahoo.com } Sent: Mon, April 11, 2011 4:26:17 PM } Subject: [Microscopy] Stuck Zeiss objective } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a 63x Plan Apo Zeiss objective that has been on an inverted microscope } for years. I noticed the other day that the barrel no longer moves and I am } pretty sure it was at one time spring loaded. Presumably crud has gotten inside } and frozen the mechanism. I tried removing the small set screw on the side of } the barrel to see if I could remove the casing and get at the mechanism and } hopefully free it but the casing did not come off easily. I presume that I } should not add lubricant/penetrant between the inner and outer barrel to free } it. I could use my strap wrench to try to free the casing. Any suggestions? } } } Dr. David Knecht } Department of Molecular and Cell Biology } Co-head Flow Cytometry and Confocal Microscopy Facility } U-3125 } 91 N. Eagleville Rd. } University of Connecticut } Storrs, CT 06269 } 860-486-2200 } 860-486-4331 (fax) } } } } } ==============================Original Headers============================== } 5, 19 -- From david.knecht-at-uconn.edu Mon Apr 11 09:22:00 2011 } 5, 19 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu } [137.99.25.234]) } 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3BEM0co016909 } 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 09:22:00 -0500 } 5, 19 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu } [137.99.46.164]) } 5, 19 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 766012D064 } 5, 19 -- for {microscopy-at-microscopy.com} ; Mon, 11 Apr 2011 10:22:00 -0400 } (EDT) } 5, 19 -- From: David Knecht {david.knecht-at-uconn.edu} } 5, 19 -- Content-Type: text/plain; charset=us-ascii } 5, 19 -- Subject: Stuck Zeiss objective } 5, 19 -- Date: Mon, 11 Apr 2011 10:22:00 -0400 } 5, 19 -- Message-Id: {298204B0-70CE-404D-825F-06A9C0A338CD-at-uconn.edu} } 5, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} } 5, 19 -- Mime-Version: 1.0 (Apple Message framework v1082) } 5, 19 -- X-Mailer: Apple Mail (2.1082) } 5, 19 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 5, 19 -- X-Virus-Status: Clean } 5, 19 -- Content-Transfer-Encoding: 8bit } 5, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3BEM0co016909 } ==============================End of - Headers============================== }
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 7, 22 -- From david.knecht-at-uconn.edu Tue Apr 12 09:02:29 2011 7, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3CE2Ttr020481 7, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Apr 2011 09:02:29 -0500 7, 22 -- Received: from d46h162.public.uconn.edu (d46h162.public.uconn.edu [137.99.46.162]) 7, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id F1E382CE43; 7, 22 -- Tue, 12 Apr 2011 10:02:28 -0400 (EDT) 7, 22 -- Subject: Re: [Microscopy] Stuck Zeiss objective 7, 22 -- Mime-Version: 1.0 (Apple Message framework v1082) 7, 22 -- Content-Type: text/plain; charset=us-ascii 7, 22 -- From: David Knecht {david.knecht-at-uconn.edu} 7, 22 -- In-Reply-To: {205964.33521.qm-at-web110808.mail.gq1.yahoo.com} 7, 22 -- Date: Tue, 12 Apr 2011 10:02:28 -0400 7, 22 -- Cc: Stephane Nizet {nizets2-at-yahoo.com} 7, 22 -- Message-Id: {0AF8C8D3-FAD6-4D59-94A8-CF89159AE20F-at-uconn.edu} 7, 22 -- References: {201104111426.p3BEQHxM022672-at-ns.microscopy.com} {205964.33521.qm-at-web110808.mail.gq1.yahoo.com} 7, 22 -- To: microscopy microscopy {microscopy-at-microscopy.com} 7, 22 -- X-Mailer: Apple Mail (2.1082) 7, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 7, 22 -- X-Virus-Status: Clean 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3CE2Ttr020481 ==============================End of - Headers==============================
I've gotten several replies, which I will summarize later, and let you all know what worked. Most of the replies, however, suggest osmium tetroxide, which I have avoided because it is corrosive to stainless steel, and I already had a very bad experience with dipping these burrs in bleach. I may try the vapors. Anyone with either experience with this? Also, I can't look for P with EDS because there is P in the adhesive. Other suggestions included bromine from Eosin and Si from HMDS. I'm an EDS newbie, so I'm naive.
Aloha, Tina
} We have a client who is a cardiac surgeon using SEM to look at surgical } burrs. These are diamond-encrusted rotary tips that are threaded up into } the heart to drill out plaque. The current question is how much tissue is } sticking to the burrs and preventing the diamonds from cutting. He } originally wanted to see how much calcified plaque was present, and this } was easy, using EDS to detect the Ca, color it in a map, and then do image } analysis to see how much area was covered. I can't figure out how to look } at non-calcified plaque. It's carbon, as are the diamonds. The adhesive is } Ni and P, on a stainless steel base. Can anyone think of something I can } "stain" the tissue with to be able to detect it with the EDS (this being } easier and faster than identifying and hand selecting with ImageJ)?
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 22 -- From tina-at-pbrc.hawaii.edu Tue Apr 12 13:58:19 2011 4, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3CIwJwV012350 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Apr 2011 13:58:19 -0500 4, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p3CIwIHO028225 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Apr 2011 08:58:19 -1000 (HST) 4, 22 -- Received: from localhost (tina-at-localhost) 4, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p3CIwI7U028221 4, 22 -- for {microscopy-at-microscopy.com} ; Tue, 12 Apr 2011 08:58:18 -1000 (HST) 4, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 22 -- Date: Tue, 12 Apr 2011 08:58:18 -1000 (HST) 4, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 22 -- X-X-Sender: tina-at-b1000 4, 22 -- To: MSA Listserver {microscopy-at-microscopy.com} 4, 22 -- Subject: RE: [Microscopy] Detecting tissue on surgical burr with SEM/EDS 4, 22 -- In-Reply-To: {00EDE41B7AFF434681877828CE8FEF7F-at-Ken} 4, 22 -- Message-ID: {Pine.GSO.4.64.1104120852380.27525-at-b1000} 4, 22 -- References: {00EDE41B7AFF434681877828CE8FEF7F-at-Ken} 4, 22 -- MIME-Version: 1.0 4, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
With our [5 year old] Zeiss 510 confocal Plan Apochromat and Neofluar objectives, once you have removed that little screw on the outer barrel, you can undo the top locking collar part of the objective by unscrewing the little top ring collar completely - this is the top bit with the blue paint striped ring around it on the outer barrel, just below the main objective top lens on the inner lens barrel, and it should unscrew off in a normal fashion and then the main inner lens barrel drops completely out from the outer casing with the large spring and washers etc... i.e. the spring mechanism is released. Once disassembled you can carefully clean all the bits [spring, washers and inner/outer barrel sides - only the inner lens barrel is really delicate and thats now separate].
This is clearly what you are trying to do [as you have removed the single outer case holding screw on the objective, that screws into a small free running slot on the inner barrel to control the up/down bit]. So it appears this top screw-on collar [blue striped bit] on your objective is stuck fast [assuming you have tried to remove it and failed - it turns in a normal unscrew thread direction I think, but can't remember if it's anti-clockwise or clockwise, I'm not at work for a few days so I'll have to check that out later].
Ironically I have had the same problem with my Zeiss 40x Plan Neofluar objective [whereas the screw collar on my 60x Plan Apochromat is quite loose and easy to remove anytime]. I've tried gripping the top collar on my 40x but it's stuck fast and won't unscrew, and I'll obviously scratch the nice finish on the metal if I try any harder [which I am not prepared to do, I leave that to the users - possibly Zeiss could provide another collar cheaply as it's only a little bit of metal, albeit nicely painted]. I could try a bit of WD-40 to see if that gets the collars screw thread released and off, and the spring mechanism apart, but again I'm loathe to use oil/solvents of that nature near the optics.
Washing things down the sides in between the outer barrel and inner lens barrel does work but I'd avoid it as you have to be extremely careful of the lens at the base [nosepiece/turret end], as liquids seem to penetrate inside the lens barrel quite easily down there, which is really bad news. Oil in particular can creep upwards and doesn't evaporate conveniently away, so I wouldn't try it [as it will keep on creeping after the objective is replaced into the nosepiece, there's a lot of space between the inner and outer casing for oil to get stuck in] - I'd concentrate on getting that top locking collar off first.
Our Zeiss confocal engineer is visiting us soon, and I'll ask him for advice on releasing a stuck screw-on collar - but obviously its anything that would be good to get a seized thread working again.
Hope this helps and makes sense [I can send a photo on thursday of our dissambled 63x objective if it doesn't].
Regards
Keith
-------------------------------------------------
Dr Keith J Morris, Molecular Cytogenetics and Microscopy Core, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, United Kingdom.
-----Original Message----- X-from: Keith Morris [mailto:kjmorris-at-well.ox.ac.uk] Sent: 12 April 2011 16:57 To: 'Keith J. Morris'
Here is the theory as I see it (correct if necessary):
The phase shift is not only due to the refraction index but also to the specimen thickness. The phase shift is much more influenced by the refraction index and specimen thickness than by the difference in traveled distance due to different diffraction angles. As to the roles of the condenser annulus and phase ring: The condenser annulus produces a ring of light, such as the uninfluenced (background) light will arrive exactly on the phase ring (this must be precisely adjusted). The phase ring is represented by a special coating (or the glass is carved) in such a way that the background light is by 1/4 phase-shifted (whether positively or negatively). Because the diffracted light is naturally shifted by about 1/4 too (this is due to the refraction index and thickness of cells), the phase interference can be destructive (dark) or additive (bright) up to 180°. But even with the phase interference occuring that way, the background light would be too intense to obtain really a good contrast of small details, so the trick is to reduce the background light by up to 90° (again in the phase ring). This is an artificial (but effective way) to increase the proportion of interference light in the image creation process. In other words, without a phase ring the phase interference would still occur (although up to 90° and not 180°) but would be so small in comparison to the intense background light that it would not give a good contrast. Because the diffracted light does not follow a ring pattern like the background light but rather diffract in all directions, it will not be influenced by the phase ring and will join the background light (which passed through the phase ring) in the image plane.
In other words the phase ring (along with the condenser annulus of course) allows the physical sorting of background and diffracted light.
PS: it is possible that I didn't use the very accurate terms but I think (and hope) that it makes the explanations easier to understand.
You explanation sounds much like many other explanations I have read, and has the same problem that led to my posting the initial question. You start out talking about refractive index and then switch to talking about diffracted light. Diffraction and refraction are quite different phenomenon and I can't see how they can be discussed as if they were interchangeable. The non-background light is shifted in path and phase either by refraction alone, diffraction alone or refraction of diffracted light or diffraction of refracted light and I am unclear which is most accurate description. Thanks- Dave
On Apr 13, 2011, at 5:27 AM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Here is the theory as I see it (correct if necessary): } } The phase shift is not only due to the refraction index but also to the specimen } thickness. The phase shift is much more influenced by the refraction index and } specimen thickness than by the difference in traveled distance due to different } diffraction angles. } As to the roles of the condenser annulus and phase ring: } The condenser annulus produces a ring of light, such as the uninfluenced } (background) light will arrive exactly on the phase ring (this must be precisely } adjusted). The phase ring is represented by a special coating (or the glass is } carved) in such a way that the background light is by 1/4 phase-shifted (whether } positively or negatively). } Because the diffracted light is naturally shifted by about 1/4 too (this is due } to the refraction index and thickness of cells), the phase interference can be } destructive (dark) or additive (bright) up to 180°. } But even with the phase interference occuring that way, the background light } would be too intense to obtain really a good contrast of small details, so the } trick is to reduce the background light by up to 90° (again in the phase ring). } This is an artificial (but effective way) to increase the proportion of } interference light in the image creation process. } In other words, without a phase ring the phase interference would still occur } (although up to 90° and not 180°) but would be so small in comparison to the } intense background light that it would not give a good contrast. } Because the diffracted light does not follow a ring pattern like the background } light but rather diffract in all directions, it will not be influenced by the } phase ring and will join the background light (which passed through the phase } ring) in the image plane. } } In other words the phase ring (along with the condenser annulus of course) } allows the physical sorting of background and diffracted light. } } PS: it is possible that I didn't use the very accurate terms but I think (and } hope) that it makes the explanations easier to understand. } } Stephane } } } ==============================Original Headers============================== } 6, 36 -- From nizets2-at-yahoo.com Wed Apr 13 04:26:57 2011 } 6, 36 -- Received: from nm3.bullet.mail.sp2.yahoo.com (nm3.bullet.mail.sp2.yahoo.com [98.139.91.73]) } 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p3D9Qud5004656 } 6, 36 -- for {microscopy-at-microscopy.com} ; Wed, 13 Apr 2011 04:26:56 -0500 } 6, 36 -- Received: from [98.139.91.66] by nm3.bullet.mail.sp2.yahoo.com with NNFMP; 13 Apr 2011 09:26:56 -0000 } 6, 36 -- Received: from [98.139.91.42] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 13 Apr 2011 09:26:56 -0000 } 6, 36 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 13 Apr 2011 09:26:56 -0000 } 6, 36 -- X-Yahoo-Newman-Property: ymail-3 } 6, 36 -- X-Yahoo-Newman-Id: 474171.24829.bm-at-omp1042.mail.sp2.yahoo.com } 6, 36 -- Received: (qmail 89287 invoked by uid 60001); 13 Apr 2011 09:26:56 -0000 } 6, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1302686816; bh=9+4yGe4RGjiHYGrwvhVaSfhSmBVxtmXVdOqqKg92Gvk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=XtBBLYI+FemMZ9jOKz7Z4HP47XMjcALgq+IYXAX+v5/9fezLumAR5c6DqJG11BDIaudeCpVKO3Z8BTY0AdvefmBvwGMNsgWCh8yad4ykU7uHWPCGaSQckaiUwdB+JE8HPRT4XPCNlrWEpGdu5wLrYFTSLwkI+d+fXYZbu01IfOk= } 6, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 36 -- s=s1024; d=yahoo.com; } 6, 36 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } 6, 36 -- b=2qLXjCSs/YoZ28ytrP9rQRAzpaXYOuisPl6hR+b8LTRt+JTGOnIbyuHqF4701jcRaQg29dOhxtWFNN2teeAb7EudGEk772HGM7e9IJPQlITj9Gp3lDc/R+BMpzen6qXCuFGrZeUz9nL68SKcwiPbUGsbsLW2wx/YG8dfyD+cros=; } 6, 36 -- Message-ID: {20074.89260.qm-at-web110813.mail.gq1.yahoo.com} } 6, 36 -- X-YMail-OSG: RItJLXYVM1nrPuFyO4yc7AsFEl15QOaPKCjmoMtEl9yN8aw } 6, 36 -- 9kw9ObEsJfcO3_tys3nuczYAipECBhmpFsjgl8Z4zYcc1c4l_Dnsz61VJJ5K } 6, 36 -- i9fTUPKuOVUBl6_ZZYnp7lLkWhlt1HR5MMuFAAke67JxDFCJYU1Aq1BH5Bei } 6, 36 -- ShL1gUyKE7ZlA87rTxdtOz1Nvzn52tjNYWzlET0iRmneoI7M5ktMkBdRggTe } 6, 36 -- 5wKoGaQzFZ1JoHv8RHrl4L.bvHaPq.VmCHDU4Eqp8QClUm6MX6KsUIpc_Q8f } 6, 36 -- MJ53E3HJWCmGHmhuFTxegkr82UVAcshdwJN45OriE4R7IAjTIxwwyvc4q5S7 } 6, 36 -- LHqcZtO8YPKzF5.WR7zxpv.xreWVuJxUKC8QfyZzIjIkKywQb9QodnFP4CVP } 6, 36 -- 4gO4pywa9OxmJhQ-- } 6, 36 -- Received: from [80.122.101.100] by web110813.mail.gq1.yahoo.com via HTTP; Wed, 13 Apr 2011 02:26:55 PDT } 6, 36 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 } 6, 36 -- References: {201104111544.p3BFi5b3007875-at-ns.microscopy.com} } 6, 36 -- Date: Wed, 13 Apr 2011 02:26:55 -0700 (PDT) } 6, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 36 -- Subject: Re: [Microscopy] Phase contrast again- diffraction vs. refraction } 6, 36 -- To: microscopy-at-microscopy.com } 6, 36 -- In-Reply-To: {201104111544.p3BFi5b3007875-at-ns.microscopy.com} } 6, 36 -- MIME-Version: 1.0 } 6, 36 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 36 -- Content-Transfer-Encoding: 8bit } 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3D9Qud5004656 } ==============================End of - Headers==============================
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 7, 22 -- From david.knecht-at-uconn.edu Wed Apr 13 09:37:58 2011 7, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3DEbwI9028721 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 13 Apr 2011 09:37:58 -0500 7, 22 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) 7, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 3175E2CF9F; 7, 22 -- Wed, 13 Apr 2011 10:37:58 -0400 (EDT) 7, 22 -- Subject: Re: [Microscopy] Re: Phase contrast again- diffraction vs. refraction 7, 22 -- Mime-Version: 1.0 (Apple Message framework v1082) 7, 22 -- Content-Type: text/plain; charset=iso-8859-1 7, 22 -- From: David Knecht {david.knecht-at-uconn.edu} 7, 22 -- In-Reply-To: {201104130927.p3D9R7LH004851-at-ns.microscopy.com} 7, 22 -- Date: Wed, 13 Apr 2011 10:37:58 -0400 7, 22 -- Cc: Stephane Nizet {nizets2-at-yahoo.com} 7, 22 -- Message-Id: {1D001954-D05A-416E-8D34-6280ED0E5C01-at-uconn.edu} 7, 22 -- References: {201104130927.p3D9R7LH004851-at-ns.microscopy.com} 7, 22 -- To: microscopy microscopy {microscopy-at-microscopy.com} 7, 22 -- X-Mailer: Apple Mail (2.1082) 7, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 7, 22 -- X-Virus-Status: Clean 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3DEbwI9028721 ==============================End of - Headers==============================
I've been thinking on this, and the only answer is "what kind of electron microscopist?" I may be somewhat diverse (or dispersed), but not nearly so much as microscopy is. So, I'm throwing this to the list and inviting any who wish to answer to respond. Please do so to the original poster at his email address. (Not to me or the list.)
Phil
} Date: Fri, 8 Apr 2011 20:14:53 -0700 } From: Niko Bellanti {niko4758-at-yahoo.com} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Friday, April 08, } 2011 at 08:14:39 PM. } } realname - Niko Bellanti } Email - niko4758-at-yahoo.com } EDUCATION - Undergraduate College } SUBJECT_OF_QUESTION - Job Details } QUESTION - What does an average day on the job entail for an } electron microscopist? If possible, several scenarios would be } helpful in helping me understand what I would be doing from day to } day. } Thank you very much. } -- Philip Oshel Microscopy Society of America Ask a Microscopist http://www.microscopy.org/microscopy/ask.cfm
==============================Original Headers============================== 5, 27 -- From oshel1pe-at-cmich.edu Wed Apr 13 15:06:33 2011 5, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3DK6XHf019638 5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Apr 2011 15:06:33 -0500 5, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 5, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p3DK6SGa015697 5, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Apr 2011 16:06:32 -0400 5, 27 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 5, 27 -- Wed, 13 Apr 2011 16:06:28 -0400 5, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Wed, 13 Apr 5, 27 -- 2011 16:06:28 -0400 5, 27 -- MIME-Version: 1.0 5, 27 -- Message-ID: {a06240809c9cbb1a29aa1-at-[141.209.160.249]} 5, 27 -- Date: Wed, 13 Apr 2011 16:06:24 -0400 5, 27 -- To: {Microscopy-at-microscopy.com} 5, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 27 -- Subject: Fwd: Ask-A-Microscopist - what does a microscopist do? 5, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 27 -- X-Originating-IP: [141.209.160.249] 5, 27 -- X-OriginalArrivalTime: 13 Apr 2011 20:06:28.0357 (UTC) FILETIME=[4629F350:01CBFA16] 5, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 27 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 5, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 27 -- X-CanItPRO-Stream: default 5, 27 -- X-Canit-Stats-ID: 02Ev86wjA - 4b1f87aeefde - 20110413 5, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Abbe defined the formation of a microscope image as the result of interferences between diffracted wave fronts. But you also have refraction due to the difference in density (refractive index). Actually you get both phenomena if you observe cells. Both phenomena must be taken into account for phase contrast because they are responsible for different parts of the technique: The phase-shift, responsible for the contrast, is due to the refraction (as I said it is not enough to create a good contrast image though, you still need to phase-shift and dim the background light).
The separation of background light from diffracted light is made possible by the diffraction phenomenon taking place in the specimen and by the presence of the phase ring at the objective (which specifically modifies the background light without affecting the diffracted light). In summary, both terms and definitely not interchangeable but they are still both playing a role in the formation of a phase-contrast image.
Regards,
Stephane
----- Original Message ---- X-from: "david.knecht-at-uconn.edu" {david.knecht-at-uconn.edu} To: nizets2-at-yahoo.com Sent: Wed, April 13, 2011 4:46:12 PM
You explanation sounds much like many other explanations I have read, and has the same problem that led to my posting the initial question. You start out talking about refractive index and then switch to talking about diffracted light. Diffraction and refraction are quite different phenomenon and I can't see how they can be discussed as if they were interchangeable. The non-background light is shifted in path and phase either by refraction alone, diffraction alone or refraction of diffracted light or diffraction of refracted light and I am unclear which is most accurate description. Thanks- Dave
On Apr 13, 2011, at 5:27 AM, nizets2-at-yahoo.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Here is the theory as I see it (correct if necessary): } } The phase shift is not only due to the refraction index but also to the } specimen } } thickness. The phase shift is much more influenced by the refraction index and
} specimen thickness than by the difference in traveled distance due to different } } } diffraction angles. } As to the roles of the condenser annulus and phase ring: } The condenser annulus produces a ring of light, such as the uninfluenced } (background) light will arrive exactly on the phase ring (this must be } precisely } } adjusted). The phase ring is represented by a special coating (or the glass is
} carved) in such a way that the background light is by 1/4 phase-shifted } (whether } } positively or negatively). } Because the diffracted light is naturally shifted by about 1/4 too (this is due } } } to the refraction index and thickness of cells), the phase interference can be
} destructive (dark) or additive (bright) up to 180°. } But even with the phase interference occuring that way, the background light } would be too intense to obtain really a good contrast of small details, so the
} trick is to reduce the background light by up to 90° (again in the phase ring). } } } This is an artificial (but effective way) to increase the proportion of } interference light in the image creation process. } In other words, without a phase ring the phase interference would still occur } (although up to 90° and not 180°) but would be so small in comparison to the } intense background light that it would not give a good contrast. } Because the diffracted light does not follow a ring pattern like the background } } } light but rather diffract in all directions, it will not be influenced by the } phase ring and will join the background light (which passed through the phase } ring) in the image plane. } } In other words the phase ring (along with the condenser annulus of course) } allows the physical sorting of background and diffracted light. } } PS: it is possible that I didn't use the very accurate terms but I think (and } hope) that it makes the explanations easier to understand. } } Stephane } } } ==============================Original Headers============================== } 6, 36 -- From nizets2-at-yahoo.com Wed Apr 13 04:26:57 2011 } 6, 36 -- Received: from nm3.bullet.mail.sp2.yahoo.com } (nm3.bullet.mail.sp2.yahoo.com [98.139.91.73]) } 6, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p3D9Qud5004656 } 6, 36 -- for {microscopy-at-microscopy.com} ; Wed, 13 Apr 2011 04:26:56 -0500 } 6, 36 -- Received: from [98.139.91.66] by nm3.bullet.mail.sp2.yahoo.com with } NNFMP; 13 Apr 2011 09:26:56 -0000 } 6, 36 -- Received: from [98.139.91.42] by tm6.bullet.mail.sp2.yahoo.com with } NNFMP; 13 Apr 2011 09:26:56 -0000 } 6, 36 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; } 13 Apr 2011 09:26:56 -0000 } 6, 36 -- X-Yahoo-Newman-Property: ymail-3 } 6, 36 -- X-Yahoo-Newman-Id: 474171.24829.bm-at-omp1042.mail.sp2.yahoo.com } 6, 36 -- Received: (qmail 89287 invoked by uid 60001); 13 Apr 2011 09:26:56 } -0000 } 6, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; } s=s1024; t=1302686816; bh=9+4yGe4RGjiHYGrwvhVaSfhSmBVxtmXVdOqqKg92Gvk=; } h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } } b=XtBBLYI+FemMZ9jOKz7Z4HP47XMjcALgq+IYXAX+v5/9fezLumAR5c6DqJG11BDIaudeCpVKO3Z8BTY0AdvefmBvwGMNsgWCh8yad4ykU7uHWPCGaSQckaiUwdB+JE8HPRT4XPCNlrWEpGdu5wLrYFTSLwkI+d+fXYZbu01IfOk= } } = } 6, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 6, 36 -- s=s1024; d=yahoo.com; } 6, 36 -- } h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } } } 6, 36 -- } b=2qLXjCSs/YoZ28ytrP9rQRAzpaXYOuisPl6hR+b8LTRt+JTGOnIbyuHqF4701jcRaQg29dOhxtWFNN2teeAb7EudGEk772HGM7e9IJPQlITj9Gp3lDc/R+BMpzen6qXCuFGrZeUz9nL68SKcwiPbUGsbsLW2wx/YG8dfyD+cros=; } } } 6, 36 -- Message-ID: {20074.89260.qm-at-web110813.mail.gq1.yahoo.com} } 6, 36 -- X-YMail-OSG: RItJLXYVM1nrPuFyO4yc7AsFEl15QOaPKCjmoMtEl9yN8aw } 6, 36 -- 9kw9ObEsJfcO3_tys3nuczYAipECBhmpFsjgl8Z4zYcc1c4l_Dnsz61VJJ5K } 6, 36 -- i9fTUPKuOVUBl6_ZZYnp7lLkWhlt1HR5MMuFAAke67JxDFCJYU1Aq1BH5Bei } 6, 36 -- ShL1gUyKE7ZlA87rTxdtOz1Nvzn52tjNYWzlET0iRmneoI7M5ktMkBdRggTe } 6, 36 -- 5wKoGaQzFZ1JoHv8RHrl4L.bvHaPq.VmCHDU4Eqp8QClUm6MX6KsUIpc_Q8f } 6, 36 -- MJ53E3HJWCmGHmhuFTxegkr82UVAcshdwJN45OriE4R7IAjTIxwwyvc4q5S7 } 6, 36 -- LHqcZtO8YPKzF5.WR7zxpv.xreWVuJxUKC8QfyZzIjIkKywQb9QodnFP4CVP } 6, 36 -- 4gO4pywa9OxmJhQ-- } 6, 36 -- Received: from [80.122.101.100] by web110813.mail.gq1.yahoo.com via } HTTP; Wed, 13 Apr 2011 02:26:55 PDT } 6, 36 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.109.295617 } 6, 36 -- References: {201104111544.p3BFi5b3007875-at-ns.microscopy.com} } 6, 36 -- Date: Wed, 13 Apr 2011 02:26:55 -0700 (PDT) } 6, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 36 -- Subject: Re: [Microscopy] Phase contrast again- diffraction vs. } refraction } 6, 36 -- To: microscopy-at-microscopy.com } 6, 36 -- In-Reply-To: {201104111544.p3BFi5b3007875-at-ns.microscopy.com} } 6, 36 -- MIME-Version: 1.0 } 6, 36 -- Content-Type: text/plain; charset=iso-8859-1 } 6, 36 -- Content-Transfer-Encoding: 8bit } 6, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3D9Qud5004656 } ==============================End of - Headers==============================
Dr. David Knecht Department of Molecular and Cell Biology Co-head Flow Cytometry and Confocal Microscopy Facility U-3125 91 N. Eagleville Rd. University of Connecticut Storrs, CT 06269 860-486-2200 860-486-4331 (fax)
==============================Original Headers============================== 7, 22 -- From david.knecht-at-uconn.edu Wed Apr 13 09:37:58 2011 7, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3DEbwI9028721 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 13 Apr 2011 09:37:58 -0500 7, 22 -- Received: from d46h164.public.uconn.edu (d46h164.public.uconn.edu [137.99.46.164]) 7, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 3175E2CF9F; 7, 22 -- Wed, 13 Apr 2011 10:37:58 -0400 (EDT) 7, 22 -- Subject: Re: [Microscopy] Re: Phase contrast again- diffraction vs. refraction 7, 22 -- Mime-Version: 1.0 (Apple Message framework v1082) 7, 22 -- Content-Type: text/plain; charset=iso-8859-1 7, 22 -- From: David Knecht {david.knecht-at-uconn.edu} 7, 22 -- In-Reply-To: {201104130927.p3D9R7LH004851-at-ns.microscopy.com} 7, 22 -- Date: Wed, 13 Apr 2011 10:37:58 -0400 7, 22 -- Cc: Stephane Nizet {nizets2-at-yahoo.com} 7, 22 -- Message-Id: {1D001954-D05A-416E-8D34-6280ED0E5C01-at-uconn.edu} 7, 22 -- References: {201104130927.p3D9R7LH004851-at-ns.microscopy.com} 7, 22 -- To: microscopy microscopy {microscopy-at-microscopy.com} 7, 22 -- X-Mailer: Apple Mail (2.1082) 7, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 7, 22 -- X-Virus-Status: Clean 7, 22 -- Content-Transfer-Encoding: 8bit 7, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3DEbwI9028721 ==============================End of - Headers==============================
This has been sitting in my draft folder for a while, so I should pass it on...
This sounds a bit like Picein wax*, often called black wax [for obvious reasons] which is soluble in benzene/turpentine, not sure about acetone though [alcohol didn't affect it] - there were varieties with different melting properties. I'm not sure if its about much as most references are 20+ years ago, and I had some supplied by Edwards Vacuum Ltd - its use is more commonly quoted the in 1930s to 1970s. Trouble is black wax is a bit of a vague generic term, and your microscopist might well have been thinking of a newer wax or brand name.
The only recent link I can find for Picein wax is from 1992. I expect Picein wax has been replaced with more modern varieties or been simply been rebranded, e.g. Edwards Apiezon wax? That sounds a bit like the name of Pat's black wax - which apparently is "an excellent material for shielding or blanking off sections of apparatus, and the compound is easily applied and can be readily removed" [just like Picein wax], see:
There is also a black wax [Mounting Wax 100, MWM100 Black Wax] listed at http://www.emsdiasum.com/microscopy/products/materials/adhesives.aspx Which might well be the wax you are interested in [and could be Picein wax in a new guise], so again Pats advice is sound.
Keith
*Picein wax is [or was] used as a vacuum sealer and insulator. I tried it as a sealant for around coverslips as it was lying about in the lab [came from Edwards the vacuum pump people, I seem to remember], and it works in a similar, if less permanent, fashion to the engine paint Glyptal enamel for sealing in large specimens onto glass slides [whole organisms] with the cover-slip say resting on a raised plastic ring and the Glyptal paint/picein wax sealing/bonding the glass and plastic ring together [Glytal paint seems far more permanent than nail varnish, assuming its constituents haven't changed] - I believe Glyptal enamel paint was also used to help seal vacuum joints but I imagine it's a lot more difficult to remove than Picein wax. Picein Black Wax was good for gluing/sealing around mica windows apparently. These days I generally use black extra strength nail varnish as it's easier to handle, I now rarely emtomb whole specimens, and archive life and space is too short for permanent slide preparations.
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: connellyps-at-nhlbi.nih.gov [mailto:connellyps-at-nhlbi.nih.gov] Sent: 08 April 2011 15:43 To: kjmorris-at-well.ox.ac.uk
Marissa,
In 1976 I started in a lab that had some sticks of black wax in the cabinet. I never used it but thought that it was possibly used to seal vacuum leaks. I remember that they were very hard and not what I would consider wax like. It had an orange label on it and I think it may have been Apizion or something similar to that printed on it. I think it may have come from Thomas or VWR as they supplied a lot of the things that I inherited there.
If they have not contacted you in response to your posting, I'd suggest you phone or email one of the EM product suppliers like SPI, Electron Microscopy Sciences, Ted Pella to name a few for they all have a vast knowledge of products that are currently available.
You might wish to post what you want to accomplish by using the black wax so we can make suggestions or go back to the person who suggested it to you and ask for the supplier information that he may have.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
The Electron Microscopy Core at the University of Missouri is hosting the 9th Annual Short Course and Workshop on Computer-Assisted Image Analysis and Measurement taught by Dr. John C. Russ on June 7-10, 2011. This popular course is intended to familiarize users of image analysis hardware and software with the fundamental principles and methods available to obtain meaningful results.
Image analysis and measurement techniques are utilized in a broad range of applications usually to extract numerical values (number, size, shape, etc.) or the location of objects. In other cases, global structural parameters such as the volume and surface of features are of interest.
The course relies heavily on tightly coupled lectures and hands-on exercises covering a wide variety of methodologies, approaches and tools, through a set of practical, step-by-step instructions to minimize the learning curve. Time will also be allotted for students to develop imaging strategies while working on their own micrographs.
No specific background is assumed, although users should already be familiar with microscopy or other imaging technologies. The software platform for the examples and exercises is Adobe Photoshop utilizing a comprehensive set of plug-ins from Reindeer Graphics. The course begins with An Introduction to Adobe Photoshop for Scientific Imaging.
Dr. Russ is the internationally acclaimed author of innumerable articles and several books on image analysis techniques and digital imaging, including the The Image Processing Handbook. He is widely known for his workshops and short courses and has helped to develop software packages to make image analysis methods more accessible to non-specialists. Many of the examples used in the course involve light or electron microscope images, but students are invited to bring their own images (TIFF files) for discussion and analysis. Ample time is provided at the end of the course for individual instruction.
The registration fee is only $1400. Enrollment is limited to assure individualized instruction. More information can be found at: www.emc.missouri.edu/ or by contacting the course coordinator Lou Ross.
Lou Ross Sr. Research Specialist University of Missouri Electron Microscopy Core Facility W136 Veterinary Medicine Building Columbia, MO 65211 573.882.4777, fax 573.884.2227 RossLM-at-missouri.edu http://www.emc.missouri.edu/
==============================Original Headers============================== 10, 28 -- From RossLM-at-missouri.edu Thu Apr 14 11:05:35 2011 10, 28 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3EG5ZRZ026892 10, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Apr 2011 11:05:35 -0500 10, 28 -- X-IronPort-Anti-Spam-Filtered: true 10, 28 -- X-IronPort-Anti-Spam-Result: Av0EAI0ap03Raua2/2dsb2JhbACkf3d3uFqIbYMYglYEhVo 10, 28 -- Received: from um-nhub02.um.umsystem.edu ([209.106.230.182]) 10, 28 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 14 Apr 2011 11:05:34 -0500 10, 28 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by 10, 28 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Thu, 14 Apr 2011 10, 28 -- 11:05:34 -0500 10, 28 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 10, 28 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com} 10, 28 -- Date: Thu, 14 Apr 2011 11:05:32 -0500 10, 28 -- Subject: 9th Annual Image Processing and Analysis Workshop, June 7-10 10, 28 -- Thread-Topic: 9th Annual Image Processing and Analysis Workshop, June 7-10 10, 28 -- Thread-Index: Acv6vcfqhtijHT3o9EmFLv7Nizj1yw== 10, 28 -- Message-ID: {C9CC857C.1BAC4%rosslm-at-missouri.edu} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- user-agent: Microsoft-Entourage/13.9.0.110114 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="us-ascii" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3EG5ZRZ026892 ==============================End of - Headers==============================
Dear Laci, When that happened to a CCD on a scope I previously worked at, a service person from GATAN carefully rinsed the surface of the scintillator with alcohol. This was a fairly risky procedure, since even this gentle process could damage the surface of the scintillator, but it was done successfully. Yours, Bill
X-from: jflaci-at-ms.sapientia.ro To: William.F.Tivol-at-aero.org
We recently discovered (yesterday), that our CCD surface is contaminated with fluid and solid particles, the problem is that is affecting image quality.
Any of You can recommend a cleaning procedure for this surface?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3EGuvw0027666 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 14 Apr 2011 11:56:57 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3EGuvvP027664; Thu, 14 Apr 2011 11:56:57 -0500
All - I'm trying to use negative stain TEM to confirm pore formation in RBC membranes treated with pore forming toxins following published methods. I'm wondering what methods people have had the most success with. If you have some experience with this and are available to discuss, please contact me.
Marcia Pitzenberger Merck & Co., Inc. WP45-104 P.O.Box 4 West Point, PA 19486-0004 Tel 215 652-9767 Fax 215 652-7758 marcia_pitzenberger-at-merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
==============================Original Headers============================== 4, 30 -- From marcia_pitzenberger-at-merck.com Thu Apr 14 11:56:57 2011 4, 30 -- Received: from wily.merck.com (wily.merck.com [155.91.38.111]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3EGuvIY027661 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Apr 2011 11:56:57 -0500 4, 30 -- X-IronPort-AV: E=Sophos;i="4.64,212,1301889600"; 4, 30 -- d="scan'208";a="402709058" 4, 30 -- Received: from unknown (HELO ipmh2.merck.com) ([54.62.195.240]) 4, 30 -- by wily.merck.com with ESMTP; 14 Apr 2011 12:56:57 -0400 4, 30 -- X-IronPort-AV: E=Sophos;i="4.64,212,1301889600"; 4, 30 -- d="scan'208";a="340826882" 4, 30 -- Received: from usctgw1104.merck.com ([54.50.132.137]) 4, 30 -- by ipmh2.merck.com with ESMTP; 14 Apr 2011 12:56:57 -0400 4, 30 -- Received: from usctmx1175.merck.com ([54.50.64.29]) by usctgw1104.merck.com with Microsoft SMTPSVC(6.0.3790.4675); 4, 30 -- Thu, 14 Apr 2011 12:56:56 -0400 4, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 4, 30 -- Content-class: urn:content-classes:message 4, 30 -- MIME-Version: 1.0 4, 30 -- Subject: TEM Pore formation in RBC Membranes 4, 30 -- Date: Thu, 14 Apr 2011 12:56:56 -0400 4, 30 -- Message-ID: {5E44A9EE4B95DF46B65532EBC60D2C1751068B-at-usctmx1175.merck.com} 4, 30 -- X-MS-Has-Attach: 4, 30 -- X-MS-TNEF-Correlator: 4, 30 -- Thread-Topic: TEM Pore formation in RBC Membranes 4, 30 -- thread-index: Acv6xPYz3914Q0cGQf+lZ2aI6NlQ7g== 4, 30 -- From: "Pitzenberger, Marcia H." {marcia_pitzenberger-at-merck.com} 4, 30 -- To: {Microscopy-at-microscopy.com} 4, 30 -- X-OriginalArrivalTime: 14 Apr 2011 16:56:57.0058 (UTC) FILETIME=[F6C12C20:01CBFAC4] 4, 30 -- Content-Type: text/plain; charset="us-ascii" 4, 30 -- Content-Transfer-Encoding: 8bit 4, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3EGuvIY027661 ==============================End of - Headers==============================
We are in the need of new camera's to fit our current microscopes for our teaching labs. We currently have Nikon Coolix 4500 cameras that attach to compound scopes (Olympus CH-30) via trinocular heads. The cameras are nice because they connect to TVs (= a bigger screen for lab partners and professors to see) and the pictures are saved to a memory card (= we don't have to have computers on every bench). The Nikon Coolpix cameras are getting old and starting to fail, and they've been discontinued and are hard to find. We would like to get new camera's for these scopes but I'm having a hard time finding ones that connect to TVs and whose pictures are saved to a memory card. Any suggestions??
Thank you!!
Michelle Shafer Whitman College Biology & BBMB shafermr-at-whitman.edu
==============================Original Headers============================== 4, 24 -- From shafermr-at-whitman.edu Thu Apr 14 18:11:03 2011 4, 24 -- Received: from mail.whitman.edu (mail.whitman.edu [199.89.174.121]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3ENB3Ox018866 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Apr 2011 18:11:03 -0500 4, 24 -- Received: from vams.whitman.edu (localhost.localdomain [127.0.0.1]) 4, 24 -- by mail.whitman.edu (Postfix) with SMTP id 3888F190D61 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Apr 2011 16:11:03 -0700 (PDT) 4, 24 -- Received: from mail.whitman.edu ([127.0.0.1]) 4, 24 -- by vams.whitman.edu ([10.1.1.203]) 4, 24 -- with SMTP (gateway) id A03BD33628F; Thu, 14 Apr 2011 16:11:03 -0700 4, 24 -- Received: from zcs-main.whitman.edu (zcs-main.whitman.edu [10.1.3.35]) 4, 24 -- by mail.whitman.edu (Postfix) with ESMTP id 34498190D61 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Apr 2011 16:11:02 -0700 (PDT) 4, 24 -- Date: Thu, 14 Apr 2011 16:11:02 -0700 (PDT) 4, 24 -- From: Michelle Shafer {shafermr-at-whitman.edu} 4, 24 -- To: Microscopy-at-Microscopy.Com 4, 24 -- Message-ID: {1692416531.510940.1302822662157.JavaMail.root-at-zcs-main.whitman.edu} 4, 24 -- In-Reply-To: {1423876205.510900.1302822590651.JavaMail.root-at-zcs-main.whitman.edu} 4, 24 -- Subject: microscope/camera systems for teaching labs 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; charset=utf-8 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Originating-IP: [10.4.1.171] 4, 24 -- X-Mailer: Zimbra 6.0.6_GA_2330.RHEL5_64 (ZimbraWebClient - FF3.0 (Mac)/6.0.6_GA_2330.RHEL5_64) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I would like to use TMV as a standard for microscope evaluation (look at layer lines in cryoEM images). I may have some at my home institution but obviously I wouldn't want to break any state or federal laws bringing it to my new institution. Does anyone know what rules govern the transport of this plant pathogen in the U.S.? Is there any alternative to TMV in terms of a known standard we can use to evalute an electron microscope with?
Thank you, Angel Paredes Director of EM services NCTR Jefferson, Arkansas 72079
Hi I can't help with the legal thing, I've been having trouble finding a supplier of TMV in the Uk so I intend to resort to growing some tobacco and infecting it from a pack of rolling tobacco ( apparently quite easy ) but I have a suggestion for an alternative -
Bovine catalase crystals will give you a nice 8.75 x 6.85nm lattice to work with Cheers Ian
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 15 April 2011 00:31 To: Portman, Ian
Central States Microscopy and Microanalysis Society Spring 2011 Meeting Friday April 22, 2011
Location: Havener Center, Missouri University of Science and Technology, Rolla, Missouri Campus map: http://www.mst.edu/documents/CampusMap.pdf Travel and parking directions included, see below No registration fee, please RSVP to Clarissa Wisner cvierret-at-mst.edu
Program 9:00 am Registration, Visit with Exhibitors, View Posters
9:45 am Introduction, Paul Carpenter, President, CSMMS
10:00 am Brian Strohmeier, Thermofisher Surface Characterization of Gunshot Residue (GSR) by X-ray Photoelectron Spectroscopy (XPS) and High Resolution Electron Microscopy
10:45 am Ryan Zeigler, Washington University Overview of Lunar Sample Analysis
11:15 am Paul Carpenter, Washington University Advances in EPMA: Problem Solving Methods
11:45 am Katherine Gibson, Washington University High Current Electron-probe Microanalysis of Ni and Co in Olivine in the NWA 773 Lunar Meteorite Clan
12:00 noon Lunch (on your own/small groups)
1:00 pm Visit Exhibitors, View Posters
1:30 pm Steve Seddio, Washington University Backscattered Electron Tomography of Zirconolite in Lunar Sample 12032,366-19.
1:45 pm Tiffany Lucas, University of Missouri Investigating virus assembly with scanning electron microscopy
2:00 pm Laurie Krupa, EDAX Ametek Advances in SDD Technology for TEM
2:30 pm Scott Sitzman, Oxford Instruments Enhancing user productivity and efficiency in EBSD
3:00 pm Pete Hayden, University of Missouri Transmission electron microscopy story of epidemic childhood adolescent obesity: Marked remodeling of tissues
4:30 pm CSMMS Business Meeting
==============================Original Headers============================== 17, 27 -- From RossLM-at-missouri.edu Fri Apr 15 14:00:14 2011 17, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 17, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3FJ0EC6011371 17, 27 -- for {microscopy-at-microscopy.com} ; Fri, 15 Apr 2011 14:00:14 -0500 17, 27 -- X-IronPort-Anti-Spam-Filtered: true 17, 27 -- X-IronPort-Anti-Spam-Result: AvsEABGVqE2Dl3nN/2dsb2JhbACmCXe8HIhthW4EhWA 17, 27 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 17, 27 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 15 Apr 2011 14:00:14 -0500 17, 27 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by 17, 27 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Fri, 15 Apr 2011 17, 27 -- 14:00:13 -0500 17, 27 -- From: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 17, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 27 -- Date: Fri, 15 Apr 2011 13:55:22 -0500 17, 27 -- Subject: CSMMS Spring Meeting 4/22/11 17, 27 -- Thread-Topic: CSMMS Spring Meeting 4/22/11 17, 27 -- Thread-Index: AQHL+56sJKr7S7+uzEqc2GnVyQcvsA== 17, 27 -- Message-ID: {3FA456C55CBDB74BA1780E6DB6A7047D9715E6C3E6-at-UM-EMAIL05.um.umsystem.edu} 17, 27 -- Accept-Language: en-US 17, 27 -- Content-Language: en-US 17, 27 -- X-MS-Has-Attach: 17, 27 -- X-MS-TNEF-Correlator: 17, 27 -- acceptlanguage: en-US 17, 27 -- Content-Type: text/plain; charset="us-ascii" 17, 27 -- MIME-Version: 1.0 17, 27 -- Content-Transfer-Encoding: 8bit 17, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3FJ0EC6011371 ==============================End of - Headers==============================
I would appreciate your contacting me if you plan to discard any JEOL 800 or 6000 series SEMs. We are trying to keep a couple of these older scopes running for training instruments and really need to have a supply of parts.
If instruments are within a day's drive than we will come and dismantle and transfer. If much further away than we will have to make arrangements for transport.
Thanks, Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
==============================Original Headers============================== 6, 28 -- From dsherman-at-purdue.edu Mon Apr 18 10:15:19 2011 6, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3IFFJEg002391 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 10:15:19 -0500 6, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 6, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p3IFFJCl010769 6, 28 -- for {microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 11:15:19 -0400 6, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.138]) by WPPEXHUB01F.purdue.lcl 6, 28 -- ([::1]) with mapi; Mon, 18 Apr 2011 11:15:19 -0400 6, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 6, 28 -- Date: Mon, 18 Apr 2011 11:15:17 -0400 6, 28 -- Subject: Salvage JEOL 800 or 6000 series SEMs 6, 28 -- Thread-Topic: Salvage JEOL 800 or 6000 series SEMs 6, 28 -- Thread-Index: Acv922x9+FQvnOw4KUCd2tZHjN5w3A== 6, 28 -- Message-ID: {C9D1CDC5.DB8A%dsherman-at-purdue.edu} 6, 28 -- Accept-Language: en-US 6, 28 -- Content-Language: en-US 6, 28 -- X-MS-Has-Attach: 6, 28 -- X-MS-TNEF-Correlator: 6, 28 -- user-agent: Microsoft-Entourage/13.9.0.110114 6, 28 -- acceptlanguage: en-US 6, 28 -- Content-Type: text/plain; charset="us-ascii" 6, 28 -- MIME-Version: 1.0 6, 28 -- X-PMX-Version: 5.5.9.388399 6, 28 -- X-PerlMx-Virus-Scanned: Yes 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3IFFJEg002391 ==============================End of - Headers==============================
I am posting the following for a colleague who has difficulty with his computer. Please reply to microscopy so that everyone can read it, including him.
Ann Fook
Ann-Fook Yang, EM Unit | Unite EM Food Safety and Quality | Salubrité et qualité des aliments Agriculture and Agri-Food Canada | Agriculture et Agroalimentaire Canada Edifice K.W. Neatby Building, 960 Carling Av | 960 Boulevard Carling, Ottawa, Ontario K1A 0C6 E-mail Address / Adresse courriel:ann-fook.yang-at-agr.gc.ca Telephone | Téléphone: 613-759-1638 Facsimile | Télécopieur: 613-759-1701 Teletypewriter | Téléimprimeur 613-773-2600 Government of Canada | Gouvernement du Canada
We are using high-pressure freezing along with freeze substitution to try preserving the structure of Arabidopsis pollen. One of the challenges is preserving the pollen kitt (tryphine) on the surface of the pollen which mostly comprises of lipids and sterol-esters. We have attempted to use just paraformaldehyde (2%) and glutaraldehyde (0.2%) in the freeze substitution fixative in the hopes that the proteinaceous components of the pollen kitt would be sufficiently fixed to retain its structure after embedding but this has clearly shown to not be the case.
Our final option would be to try varying concentrations of osmium (which is known to fix lipid components) but we are very concerned about loss of immunogenicity (which we will require from the protocol).
Does anyone have any experience with the use of osmium in low temperature freeze substitution protocols where immunogenicity is adequately preserved? We would like some feedback from the community concerning the range of concentrations that we could try as well as the compatibility of osmium with other fixatives (paraformaldehyde, glutaraldehyde). The last point is expecially important because one of our samples is whole anther and the last thing we need is black osmium metal ppt in an enclosed space.
Thank you kindly for any suggestions,
==============================Original Headers============================== 17, 22 -- From Ann-Fook.Yang-at-AGR.GC.CA Mon Apr 18 13:26:09 2011 17, 22 -- Received: from agrpazsmtp7.agr.gc.ca (agrpazsmtp7.agr.gc.ca [192.197.71.118]) 17, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3IIQ9JJ025614 17, 22 -- for {microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 13:26:09 -0500 17, 22 -- Content-class: urn:content-classes:message 17, 22 -- MIME-Version: 1.0 17, 22 -- Content-Type: text/plain; 17, 22 -- charset="iso-8859-1" 17, 22 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 22 -- Subject: Osmium, freeze substitution, and immonogenicity 17, 22 -- Date: Mon, 18 Apr 2011 14:26:08 -0400 17, 22 -- Message-ID: {773CE84BBA86484A9FEDE7741B77DDA40349713B-at-onottaxms1.AGR.GC.CA} 17, 22 -- X-MS-Has-Attach: 17, 22 -- X-MS-TNEF-Correlator: 17, 22 -- Thread-Topic: Osmium, freeze substitution, and immonogenicity 17, 22 -- Thread-Index: Acv99hYsdwTa47b4TW+jNGyiCo87PQ== 17, 22 -- From: "Yang, Ann-Fook" {Ann-Fook.Yang-at-AGR.GC.CA} 17, 22 -- To: {microscopy-at-microscopy.com} 17, 22 -- X-OriginalArrivalTime: 18 Apr 2011 18:26:09.0181 (UTC) FILETIME=[168554D0:01CBFDF6] 17, 22 -- Received-SPF: none 17, 22 -- Content-Transfer-Encoding: 8bit 17, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3IIQ9JJ025614 ==============================End of - Headers==============================
} We are using high-pressure freezing along with freeze substitution to try } preserving the structure of Arabidopsis pollen. One of the challenges is } preserving the pollen kitt (tryphine) on the surface of the pollen which } mostly comprises of lipids and sterol-esters. We have attempted to use just
} paraformaldehyde (2%) and glutaraldehyde (0.2%) in the freeze substitution } fixative in the hopes that the proteinaceous components of the pollen kitt } would be sufficiently fixed to retain its structure after embedding but this
} has clearly shown to not be the case.
first, I do not have any experience with plant material, I have to admit. I can imagine that bio-material with lots of lipids and sterol esters is difficult to preserve. Whether the material is "sufficiently fixed to retain its structure after embedding", not only depends on the aldehydes but may also depend on the solvent, and the resin. You do not write which solvent is used, acetone, or ethanol, methanol, or something else? One of the tricky tasks is to find the best solvent for substitution, along with a good time schedule. Not easy. In addition, you don't know which embedding medium is to be used - which is usually infiltrated at -30, 0 C, or even room temp, but under these conditions, the liquid, monomer embedding medium is an organic solvent, which may / will extract some of the (hydrophobic) bio-material, although we don't like this to happen ...
} Our final option would be to try varying concentrations of osmium (which is
} known to fix lipid components) but we are very concerned about loss of } immunogenicity (which we will require from the protocol).
I do not trust OsO4 too much - I have seen too many cases where "solvent" + 0.5 (0.25) % Uac, plus aldehyde plus 2-5% Water (Yes!!) has been superior to adding OsO4 in any concentration. What I would try first: solvent + 0.5% Uranyl Acetate, aldehyde, (water).
} Does anyone have any experience with the use of osmium in low temperature } freeze substitution protocols where immunogenicity is adequately preserved?
I recall that those who have tried, reported some 0.0x or up to 0.x % OsO4 (x = 1 - 2), in acetone ... See above: try to omit OsO4 completely. Only use UAc. Worth a try. Alternatively: try to freeze-substitute with resin monomer, already present at very low temp. in the solvent. Epoxy was seen to act as fixative during freeze-substitution. see Matsko and Müller 2005 JSB
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 10, 24 -- From reinhard.rachel-at-biologie.uni-regensburg.de Mon Apr 18 14:23:21 2011 10, 24 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 10, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3IJNKx1010652 10, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 14:23:20 -0500 10, 24 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 10, 24 -- by localhost (Postfix) with SMTP id 92C8B37C9 10, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 21:23:17 +0200 (CEST) 10, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 10, 24 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 75A9737C7 10, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 21:23:17 +0200 (CEST) 10, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 10, 24 -- with Novell_GroupWise; Mon, 18 Apr 2011 21:23:17 +0200 10, 24 -- Message-Id: {4DACABC3020000540000CC25-at-gwsmtp1.uni-regensburg.de} 10, 24 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 10, 24 -- Date: Mon, 18 Apr 2011 21:23:15 +0200 10, 24 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 10, 24 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 10, 24 -- Subject: Osmium, freeze substitution, and immonogenicity 10, 24 -- References: {201104181826.p3IIQPRS025938-at-ns.microscopy.com} 10, 24 -- In-Reply-To: {201104181826.p3IIQPRS025938-at-ns.microscopy.com} 10, 24 -- Mime-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=UTF-8 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] TEM soft coral
Message: Hi, I received samples of soft coral that I am processing for routine TEM. I am not pleased with the results. I used 2% glut in 0.1M Na cacodylate made in ASW, decalcified in 0.1M di Na EDTA + 2% glut in 0.1M Na cac made in ASW and postfix in osmium. I extended dehydration steps to 30 min twice in each concentration. The ultrastructure is not satisfactory, cells are vacuolated. I am looking for a protocol to improve preparation of these samples. Your input from is welcome. Thank you Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rcguerrero-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] ASM in New Orleans. Looking to share accomodation costs
Message: Greetings! I will be attending the general meeting of the American Society for Microbiology in New Orleans, LA (May 21-24). It's been really difficult to find accommodation at this time so I am looking for an attendee who would be willing to share the costs of housing with me. If interested and to discuss specifics please contact me at rcguerr-at-emory.edu.
Thanks!
Ricardo
----------------------------------------- Ricardo C. Guerrero, PhD Emory University School of Medicine Division of Pediatric Infectious Diseases
Emory Children's Center 2015 Uppergate Dr NE, Suite 560 Atlanta, GA 30322 Lab: +1 404-727-3752 Office: + 1 404-727-0039 Dept. Office: +1 404-727-5642 Fax: +1 404-727-9223
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Artur.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Artur.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Chamber vaccum problem
Message: Hi dear friends We have EPMA SX100, in which there is problem in its chamber. after opening the chamber because of sample trapping (i opened the bottom of chamber and toke out the samples), the vacuum isn't completed, is anybody have idea please help me to solve this problem. Login Host: 94.183.106.107 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 19 07:41:31 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3JCfVfo013654 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 07:41:31 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 5F395384566 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 07:41:31 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id BjPd7OR8oCT5 for {microscopy-at-microscopy.com} ; 7, 25 -- Tue, 19 Apr 2011 07:41:30 -0500 (CDT) 7, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id D3551384556 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 07:41:30 -0500 (CDT) 7, 25 -- Message-ID: {4DAD82FA.9010607-at-microscopy.com} 7, 25 -- Date: Tue, 19 Apr 2011 07:41:30 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: Artur.irani-at-yahoo.com 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Chamber vaccum problem 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nian.qing.li-at-ametek.com.cn as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nian.qing.li-at-ametek.com.cn Name: Nianqing Li
Organization: EDAX Inc
Title-Subject: [Filtered] China Application Specialist
Message: EDAX, a global leading supplier of material microanalysis solutions, is looking for an experienced Application Specialist to support growth in China. Required knowledge and skills are University Degree (preferably Ph.D.) in materials science with a thorough understanding of electron microscopy (SEM and TEM), chemistry, crystallography, the analytical techniques EDS, WDS, EBSD and Micro XRF. Should have a minimum of 3 years experience in the use of product line equipment with good teaching skills as well as strong communication skills, both verbally and in writing in Mandarin and English. For more details
Hello fellow microscopists, Has anyone ever tried potassium ferrocyanide reduced osmium in a freeze substituion cocktail (after HPF) for post embded IEM labeling of LR white or gold section? If so could you post a protocol.
sincerely, Michael Delannoy
==============================Original Headers============================== 3, 29 -- From prvs=083dc41e2=delannoy-at-jhmi.edu Tue Apr 19 09:30:51 2011 3, 29 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3JEUpdL016356 3, 29 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 09:30:51 -0500 3, 29 -- X-IronPort-AV: E=Sophos;i="4.64,239,1301889600"; 3, 29 -- d="scan'208";a="96830315" 3, 29 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 3, 29 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 19 Apr 2011 10:30:50 -0400 3, 29 -- MIME-version: 1.0 3, 29 -- Content-transfer-encoding: 7BIT 3, 29 -- Content-disposition: inline 3, 29 -- Content-type: text/plain; CHARSET=US-ASCII 3, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 3, 29 -- by commsmail.johnshopkins.edu 3, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 3, 29 -- with ESMTP id {0LJW00KU5LNEGL80-at-commsmail.johnshopkins.edu} for 3, 29 -- microscopy-at-microscopy.com; Tue, 19 Apr 2011 10:30:50 -0400 (EDT) 3, 29 -- Received: from [10.173.109.14] (Forwarded-For: [10.16.66.63]) 3, 29 -- by commsmail.johnshopkins.edu (mshttpd); Tue, 19 Apr 2011 10:30:50 -0400 3, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 3, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 29 -- Message-id: {72a0a763194e.4dad645a-at-johnshopkins.edu} 3, 29 -- Date: Tue, 19 Apr 2011 10:30:50 -0400 3, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 3, 29 -- 2009) 3, 29 -- Content-language: en 3, 29 -- Subject: reduced osmium IEM 3, 29 -- X-Accept-Language: en 3, 29 -- Priority: normal ==============================End of - Headers==============================
Dear Group - we need a calendering system that needs to be linked with our webcite here at the university. In the past, our users would submit their event and we would approve it. At one point we were able to color code the type of user. This would also be helpful for billing purposes. We could view daily, weekly, monthly and annually. Our university has changed the calendaring system so drastically that we cannot use it any longer for the purpose of scheduling. Appreciate any suggestions you may have. Thanks Barbara
==============================Original Headers============================== 1, 16 -- From maloneyb-at-fiu.edu Tue Apr 19 09:44:51 2011 1, 16 -- Received: from fiumailsmtp.fiu.edu (dithub01.ad.fiu.edu [131.94.72.22]) 1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3JEipNK032767 1, 16 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 09:44:51 -0500 1, 16 -- Received: from [131.94.220.250] (131.94.220.250) by fiumailsmtp.fiu.edu 1, 16 -- (192.168.251.13) with Microsoft SMTP Server (TLS) id 14.1.270.1; Tue, 19 Apr 1, 16 -- 2011 10:44:48 -0400 1, 16 -- Message-ID: {4DAD9FE0.70205-at-fiu.edu} 1, 16 -- Date: Tue, 19 Apr 2011 10:44:48 -0400 1, 16 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 16 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 1, 16 -- MIME-Version: 1.0 1, 16 -- To: {Microscopy-at-microscopy.com} 1, 16 -- Subject: Need Calendar system for use of our research center and its instruments 1, 16 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 1, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
First action is to wipe your finger all of the way round the "O" ring surface; do not clean with a solvent. Then clean the flat plate surface in the area where the "O" ring would sit; here you may use a solvent.
Do not grease the "O" ring
Good Luck
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 19 April 2011 13:42 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Artur.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Artur.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Chamber vaccum problem
Message: Hi dear friends We have EPMA SX100, in which there is problem in its chamber. after opening the chamber because of sample trapping (i opened the bottom of chamber and toke out the samples), the vacuum isn't completed, is anybody have idea please help me to solve this problem. Login Host: 94.183.106.107 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Apr 19 07:41:31 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3JCfVfo013654 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 07:41:31 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 5F395384566 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 07:41:31 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id BjPd7OR8oCT5 for {microscopy-at-microscopy.com} ; 7, 25 -- Tue, 19 Apr 2011 07:41:30 -0500 (CDT) 7, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id D3551384556 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Apr 2011 07:41:30 -0500 (CDT) 7, 25 -- Message-ID: {4DAD82FA.9010607-at-microscopy.com} 7, 25 -- Date: Tue, 19 Apr 2011 07:41:30 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: Artur.irani-at-yahoo.com 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Chamber vaccum problem 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Wire is one of my favorite websites, i hope you enjoy the article.
Frank
-- ************************************************************* Note: The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you, The Lincoln Electric Company **************************************************************
==============================Original Headers============================== 7, 15 -- From frank_karl-at-lincolnelectric.com Wed Apr 20 11:31:19 2011 7, 15 -- Received: from lincolnelectric.com (smtp2.lincolnelectric.com [64.109.211.115]) 7, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3KGVIE7001885 7, 15 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2011 11:31:19 -0500 7, 15 -- Subject: zworykin-demonstrates-electron-microscope 7, 15 -- To: Microscopy-at-microscopy.com 7, 15 -- X-Mailer: Lotus Notes Release 6.5.5 November 30, 2005 7, 15 -- Message-ID: {OF2FD2D1D1.FD4429D7-ON85257878.0058F413-85257878.00595270-at-lincolnelectric.com} 7, 15 -- From: Frank_Karl-at-lincolnelectric.com 7, 15 -- Date: Wed, 20 Apr 2011 12:15:35 -0400 7, 15 -- X-MIMETrack: Serialize by Router on CPNADM01/Server/LECO(Release 8.5.2FP2|March 22, 2011) at 7, 15 -- 04/20/2011 12:15:36 PM 7, 15 -- MIME-Version: 1.0 7, 15 -- Content-Type: text/plain; 7, 15 -- charset="US-ASCII" ==============================End of - Headers==============================
A recent paper in JACS (do not want to provide citation intentionally) clams to have used EDS analysis in the TEM to determine presence of about 1% of Na in ZnO single crystals. Any comments on validity and prudence of such a claim?
Krassimir Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside, CA 92521
phone 951 827 2998 fax 951 827 2489
==============================Original Headers============================== 7, 20 -- From bozhilov-at-ucr.edu Wed Apr 20 14:48:14 2011 7, 20 -- Received: from sententia.ucr.edu (sententia.ucr.edu [138.23.226.213]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3KJmEPW021315 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2011 14:48:14 -0500 7, 20 -- Received: from cfamm2.ucr.edu (cfamm2.ucr.edu [138.23.103.15]) 7, 20 -- by sententia.ucr.edu (MOS 3.10.5-GA) 7, 20 -- with ESMTP id FGD08574 (AUTH bozhilov) 7, 20 -- for {microscopy-at-microscopy.com} ; 7, 20 -- Wed, 20 Apr 2011 12:48:12 -0700 (PDT) 7, 20 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu} 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Subject: Na in ZnO 7, 20 -- Date: Wed, 20 Apr 2011 12:48:11 -0700 7, 20 -- Message-Id: {1BEBCCA7-CF17-4069-BC5F-8C478CD7511B-at-ucr.edu} 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 7, 20 -- X-Mailer: Apple Mail (2.1084) 7, 20 -- X-Junkmail-Status: score=0/50, host= 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3KJmEPW021315 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rfoley-at-uab.edu Name: Robin Foley
Organization: UAB
Title-Subject: [Filtered] ASYNC EMI problems for new SEM
Message: All, we're getting a new FEI650FEG SEM and just had the site survey. We've been running an ancient SEM in the room for many years. They've informed us that our room failed the ASYNC EMI requirements. We can go ahead and get it installed with a waiver without fixing the ASYNC EMI issues and then try and fix them later if they are a problem. Or we can try and get it fixed now.
Do any of you have experience with this sort of problem? Is it really a big problem or a little one? If it is a big problem that needs fixing, do you have recommendations on how to fix it? (Methods and before or after installation?)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both innap-at-savion.huji.ac.il as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] ASYNC EMI problems for new SEM
Message: Robin, About a year ago we had faced the same problem before installation of FEI Magellan in a newly prepared room. The room has ASYNC EMI a bit higher than the pre-installation requirement states. We made several efforts with more than one EM expert, failed to identify the source of this EMI, but we mapped all the EMI sources neiboring the room and found that we can decrease their effect by installing EM-dissipating metal plates over sorrouding walls. Then we installed the instrument and found no problem of interference at any scan condition, and the specs were OK as well. Before the installation we asked FEI about possible effects of this over-spec ASYNC EMI. According to FEI, there could be flagging at specific scan condition set. Our ASYNC EMI was about 10% higher than specs in only one direction. We decided to proceed with installation first, becuase the EMI was not too large and because FEI Magellan has extremely wide set of scan conditions at which we hoped to escaped flagging even if it will occur. Hope it will help you to make you decision. Good luck, Inna
An unusual question but experience has shown that there is a lot of unusual expertise in this crowd. I collected (under federal license), some ocular tissue from raptors that had to be euthanized. I was mostly interested in the conjunctiva but they have scleral ossicles (bones in the whites of their eyes) so I couldn't resist collecting those. After hours or more in 2% paraformaldehyde, I dissected away the globe and most extraneous tissue from the ossicles. I want to ultimately get a stereoscope image of the ossicles so I put the specimens in medium concentration NaOH for weeks. Periodically I gently scrubbed tissue off of them. They are a lot cleaner but still have a gelatinous layer (collagen?) attached to them that is difficult to remove. Any thoughts on cleaning these bones up? Naturally collagenase is a possibility although after fixation and NaOH treatment I don't know how effective the enzyme would be. I would prefer something easy like dumping them into a bucket of some solvent. Thoughts?
Thanks, Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
I posted a message a few weeks ago asking for opinions on RMC ultramicrotome. I want to thank those who replied. The information was extremely helpful.
I now need your opinion on another product. We are looking for a high vacuum coater. Two models we are looking into are Leica EM SCD500 High vacuum sputter coater (previously sold under Bal-tec with same name) and Gatan 681 High Resolution Ion Beam coater. If you own or have experience using one of these (or both) models, would you share your comments with me. Please email me at hyi-at-emory.edu. Thank you in advance.
Sincerely,
Hong
This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.
If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
==============================Original Headers============================== 8, 36 -- From hyi-at-emory.edu Thu Apr 21 12:12:45 2011 8, 36 -- Received: from mr2.cc.emory.edu (mr2.cc.emory.edu [170.140.52.91]) 8, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LHCjP0008443 8, 36 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 12:12:45 -0500 8, 36 -- Received: from EXCHEDGE2.enterprise.emory.net (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 8, 36 -- by mr2.cc.emory.edu (8.13.1/8.13.1) with ESMTP id p3LHCeqQ018708 8, 36 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 13:12:40 -0400 8, 36 -- Received: from EXCHHUB2.Enterprise.emory.net (170.140.30.54) by 8, 36 -- EXCHEDGE2.enterprise.emory.net (170.140.52.34) with Microsoft SMTP Server 8, 36 -- (TLS) id 8.3.137.0; Thu, 21 Apr 2011 13:12:37 -0400 8, 36 -- Received: from EXCHANGE12.Enterprise.emory.net ([10.128.11.12]) by 8, 36 -- EXCHHUB2.Enterprise.emory.net ([170.140.30.54]) with mapi; Thu, 21 Apr 2011 8, 36 -- 13:12:39 -0400 8, 36 -- From: "Yi, Hong" {hyi-at-emory.edu} 8, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 36 -- Date: Thu, 21 Apr 2011 13:12:39 -0400 8, 36 -- Subject: High vacuum coaters 8, 36 -- Thread-Topic: High vacuum coaters 8, 36 -- Thread-Index: AQHMAEdR2xVB6RUfgUOhC90ssfV1TA== 8, 36 -- Message-ID: {3940C1839888A54DAE85AE20F294FB690130F47C8680-at-EXCHANGE12.Enterprise.emory.net} 8, 36 -- Accept-Language: en-US 8, 36 -- Content-Language: en-US 8, 36 -- X-MS-Has-Attach: 8, 36 -- X-MS-TNEF-Correlator: 8, 36 -- acceptlanguage: en-US 8, 36 -- Content-Type: text/plain; charset="us-ascii" 8, 36 -- MIME-Version: 1.0 8, 36 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 8, 36 -- X-Emory-MailScanner-ID: p3LHCeqQ018708 8, 36 -- X-Emory-MailScanner: Found to be clean 8, 36 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, score=0, 8, 36 -- required 8, autolearn=disabled) 8, 36 -- X-Emory-MailScanner-From: hyi-at-emory.edu 8, 36 -- X-Spam-Status: No 8, 36 -- Content-Transfer-Encoding: 8bit 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LHCjP0008443 ==============================End of - Headers==============================
Tom, You can try Sodium hypochlorite. It removes pretty thick layer of collagen from etched bone/dentine in 5-20 min, but I have not tried it on fixed collagen. And you do not need to order chemicals: household bleach works fine. Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Sent: Thursday, April 21, 2011 11:56 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Bone prep - gross } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } An unusual question but experience has shown that there is a lot of } unusual expertise in this crowd. I collected (under federal license), } some ocular tissue from raptors that had to be euthanized. I was } mostly interested in the conjunctiva but they have scleral ossicles } (bones in the whites of their eyes) so I couldn't resist collecting } those. After hours or more in 2% paraformaldehyde, I dissected away } the globe and most extraneous tissue from the ossicles. I want to } ultimately get a stereoscope image of the ossicles so I put the } specimens in medium concentration NaOH for weeks. Periodically I gently } scrubbed tissue off of them. They are a lot cleaner but still have a } gelatinous layer (collagen?) attached to them that is difficult to } remove. Any thoughts on cleaning these bones up? Naturally collagenase } is a possibility although after fixation and NaOH treatment I don't } know how effective the enzyme would be. I would prefer something easy } like dumping them into a bucket ! } of some solvent. Thoughts? } } Thanks, Tom } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } } ==============================Original } Headers============================== } 6, 27 -- From PhillipsT-at-missouri.edu Thu Apr 21 11:54:42 2011 } 6, 27 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01- } missouri-out.um.umsystem.edu [209.106.229.53]) } 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p3LGsgn7024580 } 6, 27 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 11:54:42 } -0500 } 6, 27 -- X-IronPort-Anti-Spam-Filtered: true } 6, 27 -- X-IronPort-Anti-Spam-Result: } AvsEAAphsE3Raua1/2dsb2JhbAClTHe7U4hvAoMID4JdBIV0 } 6, 27 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) } 6, 27 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 21 Apr } 2011 11:54:09 -0500 } 6, 27 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by } 6, 27 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Thu, } 21 Apr 2011 } 6, 27 -- 11:54:09 -0500 } 6, 27 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 6, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 6, 27 -- Date: Thu, 21 Apr 2011 11:54:08 -0500 } 6, 27 -- Subject: Bone prep - gross } 6, 27 -- Thread-Topic: Bone prep - gross } 6, 27 -- Thread-Index: AcwARLsOB8Bjy01ZR7OFxQcY6WLhPQ== } 6, 27 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF267BAD39AE-at-UM- } EMAIL05.um.umsystem.edu} } 6, 27 -- Accept-Language: en-US } 6, 27 -- Content-Language: en-US } 6, 27 -- X-MS-Has-Attach: } 6, 27 -- X-MS-TNEF-Correlator: } 6, 27 -- acceptlanguage: en-US } 6, 27 -- Content-Type: text/plain; charset="us-ascii" } 6, 27 -- MIME-Version: 1.0 } 6, 27 -- Content-Transfer-Encoding: 8bit } 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3LGsgn7024580 } ==============================End of - } Headers==============================
Dear Krassimir, 1% is possible, but close to the limit of detectability. I presume that the EDS system has an ultrathin window or no window. As to the prudence, if the spectra show a bump at the right position, and there is no possibility of interference from another element, (I don't have access to a table of x-ray lines, but I know that Cu-L is somewhere near Na-K.) I see nothing wrong with reporting it as Na. At one time I was looking for chlorinated hydrocarbons in clay samples, and I could see a small bump where the Cl should be in some of the grains. I also saw the Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and obviously must be there. This gave me confidence that the Cl bump was also real. Yours, Bill
X-from: bozhilov-at-ucr.edu To: William.F.Tivol-at-aero.org
A recent paper in JACS (do not want to provide citation intentionally) clams to have used EDS analysis in the TEM to determine presence of about 1% of Na in ZnO single crystals. Any comments on validity and prudence of such a claim?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LJAHox026259 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 21 Apr 2011 14:10:17 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3LJAH9S026257; Thu, 21 Apr 2011 14:10:17 -0500
I have been posting replies privately. I suppose I should summarize to the list.
There should be no problem detecting Na with even a Be window detector, but I would assume this is a thin-window detector.
The big problem is an overlap with the Zn-L line, and they are measuring Na in ZnO. That will be difficult. It might be possible if they are using their own profiles for the elemental peaks under their exact conditions and calibration. If they are using the factory profiles, it is probably not feasible. We collect our spectra a little faster than the factory used for their profiles, so the peaks are a bit wider. That may be fine in the case of widely separated elements. It breaks down in cases of peak overlaps.
A follow-on post indicated that this researcher had tried mapping for Na and found it uniformly distributed. It was not at all clear if the researcher considered the Zn-L overlap with Na-K. I know our system does not deconvolve peak overlaps for mapping. I don't think it even does background corrections. Maybe new systems do with their spectral imaging. I make sure to label the Na map as Na/Zn if there is any chance of the overlap.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org] Sent: Thursday, April 21, 2011 1:48 PM To: wesaia-at-iastate.edu
Dear Krassimir, 1% is possible, but close to the limit of detectability. I presume that the EDS system has an ultrathin window or no window. As to the prudence, if the spectra show a bump at the right position, and there is no possibility of interference from another element, (I don't have access to a table of x-ray lines, but I know that Cu-L is somewhere near Na-K.) I see nothing wrong with reporting it as Na. At one time I was looking for chlorinated hydrocarbons in clay samples, and I could see a small bump where the Cl should be in some of the grains. I also saw the Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and obviously must be there. This gave me confidence that the Cl bump was also real. Yours, Bill
X-from: bozhilov-at-ucr.edu To: William.F.Tivol-at-aero.org
A recent paper in JACS (do not want to provide citation intentionally) clams to have used EDS analysis in the TEM to determine presence of about 1% of Na in ZnO single crystals. Any comments on validity and prudence of such a claim?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LJQ4l9010106 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 21 Apr 2011 14:26:04 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3LJQ4ju010104; Thu, 21 Apr 2011 14:26:04 -0500
Dear David,
I have now had a chat with our Zeiss engineer regarding your 63x times Plan Apochromat objective and our 40x Plan neofluar objective that have similar problems with the lens barrel/spring assembly. Apparently the loose top on our 63x times is a fault, and has probably been loosened by immersion oil and it shouldn't easily unscrew apart like it does, that’s no doubt why the tops on our other objectives seem stuck fast, i.e. they have been factory fitted and lock-tight sealed. Obviously immersion oil penetration or possibly solvents have released the factory locked thread on our 63x oil plan apochromat. The locking ring can be removed, but apparently this is best done at the factory as it can affect the alignment of the internal barrel optics. So disregard my previous suggestion that you may be able to take apart the objective, that’s best left to the Zeiss service department. Looking at our 63x plan apochromat that comes apart, I'm not quite sure what can be miss aligned when you screw it back together (might be because the inner lens barrel with it's simple retaining clip is exposed), but Id stick to the Zeiss advice in any case (besides I doubt most could easily remove the screw top anyway as they are so well screwed on).
So the only option for your stuck retractable top is to risk putting solvents or oil down the inside of the barrel yourself, as you suggest, or send it back to Zeiss and let them take the objective apart as described and clean/realign it for you.
If the spring mechanism is stuck fast, that doesnt sound like immersion oil contamination, sounds more like a hard set mountant has crept in which might be more of a problem, as only its original solvent will easily shift it (i.e. toluene if its Histomount, but you probably wouldn't know which hard-set mountant). With immersion oil contamination (using inverted live cell optics) you just take the objective out and leave it on a tissue for 24h or so to let all the oil drip/flow out (you can get 0.5ml of immersion oil into that barrel, and occasionally our users have done just that, and with our inverted microscope it can easily drip onto the microscope's internal optics/mechanics).
Pouring organic solvents or oil down the inner tube to dissolve/dislodge whatever has stuck to your internal spring is a bit more fraught as solvents definitely do seem to creep inside the internal optics at the base if you overdo it. However hopefully with the objective upright on tissues, small amounts of whatever [oil or solvents] sent down the barrel will simply drip out onto the tissue and bypass the back lens assembly completely (as its designed to do with immersion oil excesses). I have done it occasionally, but I find all very worrying though, and sending your 'gummed up' £5,000 objective back to Zeiss for dismantling and cleaning might be an easier option, as the objective might be stuck down slightly from its correct position and whatever has stuck it down might be tricky to remove compared to immersion oil. I guess if you can free the spring though, the fact that there's still some hardened mountant still glued to the spring probably won't affect its optical quality provided the top can rise up fully.
I would try using small disposable hair bands around the tops of the objective if your objectives are in inverted configuration [as recommended to me by a Zeiss engineer]. They really do seem to reduce the flow of immersion oil down into the inner tube area and it would have prevented any hard set mountant or whatever getting inside and onto the spring assembly in the first place. The hair band doesn't affect the retraction of the lens, as the inner barrel just slides past it. I have a photo of a hair band in use here on our 63x
I did think the hair band would look a bit naff, but it looks OK as its all hidden below the stage anyway, and more importantly it does seem to offer some useful oil/mountant protection for those using inverted microscopes, as otherwise fluids flow too easily into the crevice created by the retractable inner barrel, particularly with the stage 37oC incubator on.
Happy Easter
Regards
Keith
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: david.knecht-at-uconn.edu [mailto:david.knecht-at-uconn.edu] Sent: 12 April 2011 15:10 To: kjmorris-at-well.ox.ac.uk
Those discussions were about removing objectives that were stuck in the turret. That was not a problem here. What is stuck is the spring loaded barrel of the objective. I am trying to find out if putting some penetrant on the barrel will have any chance of getting to the optics. Dave
-------------------------------------------------
Dr Keith J Morris Molecular Cytogenetics and Microscopy Core The Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom
It is pretty obvious from the paper's supporting documents that the EDS/EDX spectrum is misidentified: http://pubs.acs.org/doi/suppl/10.1021/ja908521s/suppl_file/ja908521s_si_001.pdf
NaK 1.04KeV ZnL 1.01KeV This is a common overlap, that like many people, I experience several times a week.
I hope the authors print a retraction.
-JQuinn
} A recent paper in JACS (do not want to provide citation intentionally) } clams to have used EDS analysis in the TEM to determine presence of about } 1% of Na in ZnO single crystals. Any comments on validity and prudence of } such a claim? } } Krassimir Bozhilov
==============================Original Headers============================== 13, 16 -- From jquinn-at-ms.cc.sunysb.edu Thu Apr 21 15:35:34 2011 13, 16 -- Received: from mailrelay.stonybrook.edu (mrs.stonybrook.edu [129.49.1.206]) 13, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LKZXpL027934 13, 16 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 15:35:34 -0500 13, 16 -- Received: from happy-b95c68896.ms.cc.sunysb.edu (ceas2064.matscieng.sunysb.edu [129.49.36.224]) 13, 16 -- by mailrelay.stonybrook.edu (8.14.2/8.14.2) with ESMTP id p3LKZTBl019097 13, 16 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:35:33 -0400 (EDT) 13, 16 -- Message-Id: {201104212035.p3LKZTBl019097-at-mailrelay.stonybrook.edu} 13, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 13, 16 -- Date: Thu, 21 Apr 2011 16:35:13 -0400 13, 16 -- To: microscopy-at-microscopy.com 13, 16 -- From: Jim Quinn {jquinn-at-ms.cc.sunysb.edu} 13, 16 -- Subject: Fwd: [Microscopy] Na in ZnO 13, 16 -- Mime-Version: 1.0 13, 16 -- Content-Type: text/plain; charset="us-ascii" 13, 16 -- X-Brightmail-Tracker: AAAAAQAAAUA= ==============================End of - Headers==============================
Except of course for the possibility of the doublet of Oxygen K (0.523 x 2 = 1.046 KeV) The sample is ZnO!
Peter I.
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Peter Ingram Sr. Physicist Adj. Professor of Pathology Duke University Medical Center PO Box 90319 DURHAM NC 27708-0319
2. Would using a variety of kV's using EDS give a better identification?
Thanks,
John Bozzola
----------------------------------------------------- } } } Except  of course for the possibility of the doublet of Oxygen K } (0.523 x 2 = 1.046 KeV)  The sample is ZnO! } } Peter I. } } } Dear Krassimir, } }     1% is possible, but close to the limit of detectability.  I } } presume that the EDS system has an ultrathin window or no window.  As to } } the prudence, if the spectra show a bump at the right position, and there } } is no possibility of interference from another element, (I don't have } } access to a table of x-ray lines, but I know that Cu-L is somewhere near } } Na-K.) I see nothing wrong with reporting it as Na.  At one time I was } } looking for chlorinated hydrocarbons in clay samples, and I could see a } } small bump where the Cl should be in some of the grains.  I also saw the } } Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and } } obviously must be there.  This gave me confidence that the Cl bump was } } also real. } }                     Yours, } }                       Bill } } } } } } } } X-from:  bozhilov-at-ucr.edu } } To:   William.F.Tivol-at-aero.org } } Date:  04/20/2011 01:02 PM } } Subject:     [Microscopy] Na in ZnO } } } } } } } } A recent paper in JACS (do not want to provide citation intentionally) } } clams to have used EDS analysis in the TEM to determine presence of about } } 1% of Na in ZnO single crystals. Any comments on validity and prudence of } } such a claim? } } } } Krassimir Bozhilov } } } } } } } } } -- } Peter Ingram } Sr. Physicist } Adj. Professor of Pathology } Duke University Medical Center } PO Box 90319 } DURHAM  NC  27708-0319
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 9, 20 -- From bozzola-at-siu.edu Thu Apr 21 15:54:40 2011 9, 20 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LKsdIH027866 9, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 15:54:40 -0500 9, 20 -- Received: by iyh42 with SMTP id 42so114329iyh.0 9, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 13:54:39 -0700 (PDT) 9, 20 -- MIME-Version: 1.0 9, 20 -- Received: by 10.42.108.134 with SMTP id h6mr189443icp.13.1303419279499; Thu, 9, 20 -- 21 Apr 2011 13:54:39 -0700 (PDT) 9, 20 -- Received: by 10.42.167.67 with HTTP; Thu, 21 Apr 2011 13:54:39 -0700 (PDT) 9, 20 -- In-Reply-To: {201104212044.p3LKi8T2011407-at-ns.microscopy.com} 9, 20 -- References: {201104212044.p3LKi8T2011407-at-ns.microscopy.com} 9, 20 -- Date: Thu, 21 Apr 2011 15:54:39 -0500 9, 20 -- Message-ID: {BANLkTik4kyX+XLpwwwYmJfkP3Js4gVO2DA-at-mail.gmail.com} 9, 20 -- Subject: Re: [Microscopy] Re: Na in ZnO 9, 20 -- From: John Bozzola {bozzola-at-siu.edu} 9, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 9, 20 -- Content-Type: text/plain; charset=UTF-8 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LKsdIH027866 ==============================End of - Headers==============================
On 4/21/2011 3:55 PM, bozzola-at-siu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Follow-up questions to Peter and Bill: } } 1. Would WDS give a definitive identification? } } 2. Would using a variety of kV's using EDS give a better identification?
I'll add a third question: why is the Zn K to Zn L ratio so different in the two EDS spectra in the supporting information? The ratio is much greater than 1 for the first spectrum and much less than 1 for the second spectrum.
Best wishes, Paul
} } Thanks, } } John Bozzola } } ----------------------------------------------------- } } } } } } Except  of course for the possibility of the doublet of Oxygen K } } (0.523 x 2 = 1.046 KeV)  The sample is ZnO! } } } } Peter I. } } } } } Dear Krassimir, } } }     1% is possible, but close to the limit of detectability.  I } } } presume that the EDS system has an ultrathin window or no window.  As to } } } the prudence, if the spectra show a bump at the right position, and there } } } is no possibility of interference from another element, (I don't have } } } access to a table of x-ray lines, but I know that Cu-L is somewhere near } } } Na-K.) I see nothing wrong with reporting it as Na.  At one time I was } } } looking for chlorinated hydrocarbons in clay samples, and I could see a } } } small bump where the Cl should be in some of the grains.  I also saw the } } } Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and } } } obviously must be there.  This gave me confidence that the Cl bump was } } } also real. } } }                     Yours, } } }                       Bill } } } } } } } } } } } } X-from:  bozhilov-at-ucr.edu } } } To:   William.F.Tivol-at-aero.org } } } Date:  04/20/2011 01:02 PM } } } Subject:     [Microscopy] Na in ZnO } } } } } } } } } } } } A recent paper in JACS (do not want to provide citation intentionally) } } } clams to have used EDS analysis in the TEM to determine presence of about } } } 1% of Na in ZnO single crystals. Any comments on validity and prudence of } } } such a claim? } } } } } } Krassimir Bozhilov } } } } } } } } } } } } } } } -- } } Peter Ingram } } Sr. Physicist } } Adj. Professor of Pathology } } Duke University Medical Center } } PO Box 90319 } } DURHAM  NC  27708-0319 } }
-- Paul Voyles Materials Science and Engineering University of Wisconsin, Madison 1509 University Ave, Rm 223 Madison, WI 53706-1595 voice: (608) 265-6740 fax: (608) 262-8353 voyles-at-engr.wisc.edu http://tem.msae.wisc.edu
==============================Original Headers============================== 7, 26 -- From voyles-at-engr.wisc.edu Thu Apr 21 16:03:03 2011 7, 26 -- Received: from smtp-out.cae.wisc.edu (smtp-out.cae.wisc.edu [144.92.12.60]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LL326T011724 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 7, 26 -- Received: from smtp.cae.wisc.edu (smtp.cae.wisc.edu [IPv6:2607:f388:1082:0:ac8d:e958:beb5:d993]) 7, 26 -- by smtp-out.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL32kW001354 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 7, 26 -- Received: from [IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65] ([IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65]) 7, 26 -- (authenticated bits=0) 7, 26 -- by smtp.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL31Hq005050 7, 26 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 7, 26 -- X-Virus-Status: Clean 7, 26 -- X-Virus-Scanned: clamav-milter 0.96.5 at smtp.cae.wisc.edu 7, 26 -- Message-ID: {4DB09B8D.3040903-at-engr.wisc.edu} 7, 26 -- Date: Thu, 21 Apr 2011 16:03:09 -0500 7, 26 -- From: Paul Voyles {voyles-at-engr.wisc.edu} 7, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 7, 26 -- MIME-Version: 1.0 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Subject: Re: [Microscopy] Na in ZnO 7, 26 -- References: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
S3c is an SEM spectrum and S5d is a TEM spectrum
Alan W Nicholls, PhD Interim Associate Director - RRC Director of Research Service Facility (Electron Microscopy) Research Resources Center - East (M/C 337) Room 110 Science and Engineering South Building The University of Illinois at Chicago 845 West Taylor St Chicago, IL 60607-7058
I am basing my comments on the article citation provided by Jim Quinn. http://pubs.acs.org/doi/suppl/10.1021/ja908521s/suppl_file/ja908521s_si_001.pdf
WDS should give a more definitive identification I am in the SEM lab now and don't know off the top of my head how well the WDS resolves Na-K and Zn-L. I know it does very well with Mo-L and S-K. I could check when I am back in the probe lab.
I doubt that different voltages would help much. It would alter the ratio between the lines at 9 keV and 1 keV, but it would not help to differentiate the Na and Zn portions of the peak near 1 keV.
Some of the peak at 1 keV will be due to an Oxygen sum peak, but sum peaks will be only fractions of the main peaks and the O peak at 0.53 keV is much smaller than the peak at 1 keV, so the portion due to O contribution is probably negligible.
Alan Nicholls suggested that the EDS spectrum in Fig S3-c is from an SEM while the spectrum in S5-d is from a TEM. That may be, but the images in part-a of the figures are both TEM images. The other graphics look the same. I would suppose the spectra are both from a TEM.
I can live with the mislabeling in the figures, but the fact that the peak at 1 kV is higher in the sample that was not doped with Na than in the one doped with Na would normally lead me to conclude that there is more Na in the undoped sample than in the doped one. I would normally except the Zn L and K lines to have a fixed ratio.
However, the background signal looks markedly different in the two spectra. Figure S3-c has background visible across the energy range. The O peak looks to be about 10% of the Zn-K peak. Figure S5-d shows very little background around 5 keV. The O peak is more than 50% of the Zn-K peak. Note that the ratio of O intensity to the intensity of the peak at 1 keV is roughly the same in both spectra. That tells me that the spectrum in S3-c was from an area facing more away from the EDS detector than the area used for S5-d. There is no definite difference in the peak at 1.0 keV due to the presence of Na.
The plots in S3-d prove nothing. They are predominantly from the Zn-L line.
Indeed, some serious revisions or retractions are in order.
Warren S.
-----Original Message----- X-from: voyles-at-engr.wisc.edu [mailto:voyles-at-engr.wisc.edu] Sent: Thursday, April 21, 2011 4:03 PM To: wesaia-at-iastate.edu
On 4/21/2011 3:55 PM, bozzola-at-siu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Follow-up questions to Peter and Bill: } } 1. Would WDS give a definitive identification? } } 2. Would using a variety of kV's using EDS give a better identification?
I'll add a third question: why is the Zn K to Zn L ratio so different in the two EDS spectra in the supporting information? The ratio is much greater than 1 for the first spectrum and much less than 1 for the second spectrum.
Best wishes, Paul
} } Thanks, } } John Bozzola } } ----------------------------------------------------- } } } } } } Except  of course for the possibility of the doublet of Oxygen K } } (0.523 x 2 = 1.046 KeV)  The sample is ZnO! } } } } Peter I. } } } } } Dear Krassimir, } } }     1% is possible, but close to the limit of detectability.  I } } } presume that the EDS system has an ultrathin window or no window.  As to } } } the prudence, if the spectra show a bump at the right position, and there } } } is no possibility of interference from another element, (I don't have } } } access to a table of x-ray lines, but I know that Cu-L is somewhere near } } } Na-K.) I see nothing wrong with reporting it as Na.  At one time I was } } } looking for chlorinated hydrocarbons in clay samples, and I could see a } } } small bump where the Cl should be in some of the grains.  I also saw the } } } Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and } } } obviously must be there.  This gave me confidence that the Cl bump was } } } also real. } } }                     Yours, } } }                       Bill } } } } } } } } } } } } X-from:  bozhilov-at-ucr.edu } } } To:   William.F.Tivol-at-aero.org } } } Date:  04/20/2011 01:02 PM } } } Subject:     [Microscopy] Na in ZnO } } } } } } } } } } } } A recent paper in JACS (do not want to provide citation intentionally) } } } clams to have used EDS analysis in the TEM to determine presence of about } } } 1% of Na in ZnO single crystals. Any comments on validity and prudence of } } } such a claim? } } } } } } Krassimir Bozhilov } } } } } } } } } } } } } } } -- } } Peter Ingram } } Sr. Physicist } } Adj. Professor of Pathology } } Duke University Medical Center } } PO Box 90319 } } DURHAM  NC  27708-0319 } }
-- Paul Voyles Materials Science and Engineering University of Wisconsin, Madison 1509 University Ave, Rm 223 Madison, WI 53706-1595 voice: (608) 265-6740 fax: (608) 262-8353 voyles-at-engr.wisc.edu http://tem.msae.wisc.edu
==============================Original Headers============================== 7, 26 -- From voyles-at-engr.wisc.edu Thu Apr 21 16:03:03 2011 7, 26 -- Received: from smtp-out.cae.wisc.edu (smtp-out.cae.wisc.edu [144.92.12.60]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LL326T011724 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 7, 26 -- Received: from smtp.cae.wisc.edu (smtp.cae.wisc.edu [IPv6:2607:f388:1082:0:ac8d:e958:beb5:d993]) 7, 26 -- by smtp-out.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL32kW001354 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 7, 26 -- Received: from [IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65] ([IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65]) 7, 26 -- (authenticated bits=0) 7, 26 -- by smtp.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL31Hq005050 7, 26 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 7, 26 -- X-Virus-Status: Clean 7, 26 -- X-Virus-Scanned: clamav-milter 0.96.5 at smtp.cae.wisc.edu 7, 26 -- Message-ID: {4DB09B8D.3040903-at-engr.wisc.edu} 7, 26 -- Date: Thu, 21 Apr 2011 16:03:09 -0500 7, 26 -- From: Paul Voyles {voyles-at-engr.wisc.edu} 7, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 7, 26 -- MIME-Version: 1.0 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Subject: Re: [Microscopy] Na in ZnO 7, 26 -- References: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} 7, 26 -- In-Reply-To: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 26 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Dear Peter, John, Paul, and Warren, I must admit that I haven't used WDS, but I think it has the energy resolution (equivalently, wavelength resolution) to resolve [almost] all overlaps. Since it works by diffraction from a bent crystal, a sufficient camera length would resolve the smallest difference in Bragg angles. Perhaps a WDS expert can comment on what is available in commercial apparatus. I don't think that varying the kV would help, since the overvoltage would be the same for the two lines to be separated, so only the intensities of the lines would vary, not their ratio. The occurrence of two O x-rays can be investigated by lowering the beam current so that the x-ray flux to the detector is low enough so no two O x-rays will enter the detector at intervals shorter than its response time. I think Warren's discussion of the differences among the spectra is definitive. Yours, Bill
X-from: voyles-at-engr.wisc.edu To: William.F.Tivol-at-aero.org
On 4/21/2011 3:55 PM, bozzola-at-siu.edu wrote: } } Follow-up questions to Peter and Bill: } } 1. Would WDS give a definitive identification? } } 2. Would using a variety of kV's using EDS give a better identification?
I'll add a third question: why is the Zn K to Zn L ratio so different in the two EDS spectra in the supporting information? The ratio is much greater than 1 for the first spectrum and much less than 1 for the second spectrum.
Best wishes, Paul
} } Thanks, } } John Bozzola } } } } } Except  of course for the possibility of the doublet of Oxygen K } } (0.523 x 2 = 1.046 KeV)  The sample is ZnO! } } } } Peter I. } }
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LMjfew012782 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 21 Apr 2011 17:45:41 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3LMjf1V012780; Thu, 21 Apr 2011 17:45:41 -0500
I completely agree with Warren's comments below. Just I need to point out that all these considerations hold true for analysis of flat bulk specimens (SEM and microprobe).
EDS analysis of thin specimens in the TEM is strongly dependent on sample thickness, geometry of the particle and position of the point of analysis with respect to the grid bars of the supporting TEM grid. Quantification and comparison of peak profile and intensity for low energy L lines with high energy K lines is meaningless unless proper absorption correction and thickness are taken into account.
Also when analysis is done of a particle close to the TEM grid bars secondary scattering and fluorescence from grid bar material changes the peak intensities ratios between low energy (below ~ 1.5 KeV) and higher energy peaks. Thus quantitative comparison of peak intensities among different family of lines cannot be used as a reliable factor in the TEM.
Krassimir.
On Apr 21, 2011, at 3:04 PM, wesaia-at-iastate.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am basing my comments on the article citation provided by Jim Quinn. } http://pubs.acs.org/doi/suppl/10.1021/ja908521s/suppl_file/ja908521s_si_001.pdf } } WDS should give a more definitive identification I am in the SEM lab now and don't know off the top of my head how well the WDS resolves Na-K and Zn-L. I know it does very well with Mo-L and S-K. I could check when I am back in the probe lab. } } I doubt that different voltages would help much. It would alter the ratio between the lines at 9 keV and 1 keV, but it would not help to differentiate the Na and Zn portions of the peak near 1 keV. } } Some of the peak at 1 keV will be due to an Oxygen sum peak, but sum peaks will be only fractions of the main peaks and the O peak at 0.53 keV is much smaller than the peak at 1 keV, so the portion due to O contribution is probably negligible. } } Alan Nicholls suggested that the EDS spectrum in Fig S3-c is from an SEM while the spectrum in S5-d is from a TEM. That may be, but the images in part-a of the figures are both TEM images. The other graphics look the same. I would suppose the spectra are both from a TEM. } } I can live with the mislabeling in the figures, but the fact that the peak at 1 kV is higher in the sample that was not doped with Na than in the one doped with Na would normally lead me to conclude that there is more Na in the undoped sample than in the doped one. I would normally except the Zn L and K lines to have a fixed ratio. } } However, the background signal looks markedly different in the two spectra. Figure S3-c has background visible across the energy range. The O peak looks to be about 10% of the Zn-K peak. Figure S5-d shows very little background around 5 keV. The O peak is more than 50% of the Zn-K peak. Note that the ratio of O intensity to the intensity of the peak at 1 keV is roughly the same in both spectra. That tells me that the spectrum in S3-c was from an area facing more away from the EDS detector than the area used for S5-d. There is no definite difference in the peak at 1.0 keV due to the presence of Na. } } The plots in S3-d prove nothing. They are predominantly from the Zn-L line. } } Indeed, some serious revisions or retractions are in order. } } Warren S. } } -----Original Message----- } X-from: voyles-at-engr.wisc.edu [mailto:voyles-at-engr.wisc.edu] } Sent: Thursday, April 21, 2011 4:03 PM } To: wesaia-at-iastate.edu } Subject: [Microscopy] Re: Na in ZnO } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } On 4/21/2011 3:55 PM, bozzola-at-siu.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Follow-up questions to Peter and Bill: } } } } 1. Would WDS give a definitive identification? } } } } 2. Would using a variety of kV's using EDS give a better identification? } } I'll add a third question: why is the Zn K to Zn L ratio so different in } the two EDS spectra in the supporting information? The ratio is much } greater than 1 for the first spectrum and much less than 1 for the } second spectrum. } } Best wishes, } Paul } } } } } } Thanks, } } } } John Bozzola } } } } ----------------------------------------------------- } } } } } } } } } Except B of course for the possibility of the doublet of Oxygen K } } } (0.523 x 2 = 1.046 KeV) B The sample is ZnO! } } } } } } Peter I. } } } } } } } Dear Krassimir, } } } } B B B B 1% is possible, but close to the limit of detectability. B I } } } } presume that the EDS system has an ultrathin window or no window. B As to } } } } the prudence, if the spectra show a bump at the right position, and there } } } } is no possibility of interference from another element, (I don't have } } } } access to a table of x-ray lines, but I know that Cu-L is somewhere near } } } } Na-K.) I see nothing wrong with reporting it as Na. B At one time I was } } } } looking for chlorinated hydrocarbons in clay samples, and I could see a } } } } small bump where the Cl should be in some of the grains. B I also saw the } } } } Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and } } } } obviously must be there. B This gave me confidence that the Cl bump was } } } } also real. } } } } B B B B B B B B B B B B B B B B B B B B Yours, } } } } B B B B B B B B B B B B B B B B B B B B B B Bill } } } } } } } } } } } } } } } } X-from: B bozhilov-at-ucr.edu } } } } To: B B William.F.Tivol-at-aero.org } } } } Date: B 04/20/2011 01:02 PM } } } } Subject: B B B B [Microscopy] Na in ZnO } } } } } } } } } } } } } } } } A recent paper in JACS (do not want to provide citation intentionally) } } } } clams to have used EDS analysis in the TEM to determine presence of about } } } } 1% of Na in ZnO single crystals. Any comments on validity and prudence of } } } } such a claim? } } } } } } } } Krassimir Bozhilov } } } } } } } } } } } } } } } } } } } } } -- } } } Peter Ingram } } } Sr. Physicist } } } Adj. Professor of Pathology } } } Duke University Medical Center } } } PO Box 90319 } } } DURHAM B NC B 27708-0319 } } } } } } } -- } Paul Voyles } Materials Science and Engineering } University of Wisconsin, Madison } 1509 University Ave, Rm 223 } Madison, WI 53706-1595 } voice: (608) 265-6740 } fax: (608) 262-8353 } voyles-at-engr.wisc.edu } http://tem.msae.wisc.edu } } ==============================Original Headers============================== } 7, 26 -- From voyles-at-engr.wisc.edu Thu Apr 21 16:03:03 2011 } 7, 26 -- Received: from smtp-out.cae.wisc.edu (smtp-out.cae.wisc.edu [144.92.12.60]) } 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LL326T011724 } 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 } 7, 26 -- Received: from smtp.cae.wisc.edu (smtp.cae.wisc.edu [IPv6:2607:f388:1082:0:ac8d:e958:beb5:d993]) } 7, 26 -- by smtp-out.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL32kW001354 } 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 } 7, 26 -- Received: from [IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65] ([IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65]) } 7, 26 -- (authenticated bits=0) } 7, 26 -- by smtp.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL31Hq005050 } 7, 26 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) } 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 } 7, 26 -- X-Virus-Status: Clean } 7, 26 -- X-Virus-Scanned: clamav-milter 0.96.5 at smtp.cae.wisc.edu } 7, 26 -- Message-ID: {4DB09B8D.3040903-at-engr.wisc.edu} } 7, 26 -- Date: Thu, 21 Apr 2011 16:03:09 -0500 } 7, 26 -- From: Paul Voyles {voyles-at-engr.wisc.edu} } 7, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 } 7, 26 -- MIME-Version: 1.0 } 7, 26 -- To: Microscopy-at-microscopy.com } 7, 26 -- Subject: Re: [Microscopy] Na in ZnO } 7, 26 -- References: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} } 7, 26 -- In-Reply-To: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} } 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 26 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 24, 31 -- From wesaia-at-iastate.edu Thu Apr 21 16:59:45 2011 } 24, 31 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) } 24, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LLxixI012420 } 24, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:59:44 -0500 } 24, 31 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702a.its.iastate.edu [10.10.87.140]) } 24, 31 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id p3LLxemT015607 } 24, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:59:44 -0500 } 24, 31 -- Received: from EXITS711.its.iastate.edu ([10.10.87.143]) by } 24, 31 -- EXITS702A.its.iastate.edu ([10.10.87.140]) with mapi; Thu, 21 Apr 2011 } 24, 31 -- 16:59:42 -0500 } 24, 31 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} } 24, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 24, 31 -- Date: Thu, 21 Apr 2011 17:04:22 -0500 } 24, 31 -- Subject: RE: [Microscopy] Re: Na in ZnO } 24, 31 -- Thread-Topic: [Microscopy] Re: Na in ZnO } 24, 31 -- Thread-Index: AcwAZ45WC7HEjqL7QKuYM15TmJ8+/gABU70g } 24, 31 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F13580E1B30-at-EXITS711.its.iastate.edu} } 24, 31 -- References: {201104212103.p3LL3Onp012628-at-ns.microscopy.com} } 24, 31 -- In-Reply-To: {201104212103.p3LL3Onp012628-at-ns.microscopy.com} } 24, 31 -- Accept-Language: en-US } 24, 31 -- Content-Language: en-US } 24, 31 -- X-MS-Has-Attach: } 24, 31 -- X-MS-TNEF-Correlator: } 24, 31 -- acceptlanguage: en-US } 24, 31 -- Content-Type: text/plain; charset="iso-8859-1" } 24, 31 -- MIME-Version: 1.0 } 24, 31 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.4.21.214819 } 24, 31 -- X-ISUMailhub-test: Gauge=XXIII, Probability=23%, Report=' } 24, 31 -- RATWARE_RCVD_BONUS_SPC 2.0, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, DATE_TZ_NA 0, FROM_NAME_PHRASE 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HIGHBITS 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' } 24, 31 -- Content-Transfer-Encoding: 8bit } 24, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LLxixI012420 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 22 -- From bozhilov-at-ucr.edu Thu Apr 21 17:45:41 2011 11, 22 -- Received: from sententia.ucr.edu (sententia.ucr.edu [138.23.226.213]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LMjegJ012777 11, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 17:45:40 -0500 11, 22 -- Received: from cfamm2.ucr.edu (cfamm2.ucr.edu [138.23.103.15]) 11, 22 -- by sententia.ucr.edu (MOS 3.10.5-GA) 11, 22 -- with ESMTP id FGH04693 (AUTH bozhilov); 11, 22 -- Thu, 21 Apr 2011 15:45:38 -0700 (PDT) 11, 22 -- Content-Type: text/plain; charset=us-ascii 11, 22 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 22 -- Subject: Re: [Microscopy] Na in ZnO 11, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu} 11, 22 -- In-Reply-To: {201104212204.p3LM4jJS021248-at-ns.microscopy.com} 11, 22 -- Date: Thu, 21 Apr 2011 15:45:38 -0700 11, 22 -- Message-Id: {3A23718C-947A-4AED-AC1B-B1E1C732B976-at-ucr.edu} 11, 22 -- References: {201104212204.p3LM4jJS021248-at-ns.microscopy.com} 11, 22 -- To: "Warren E [BIOTC] Straszheim" {wesaia-at-iastate.edu} , 11, 22 -- microscopy-at-microscopy.com 11, 22 -- X-Mailer: Apple Mail (2.1084) 11, 22 -- X-Junkmail-Status: score=0/50, host= 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LMjegJ012777 ==============================End of - Headers==============================
Also, the line profiles in Figure 1.3 are clearly just the thickness profiles of the cylindrical wire. EELS presents no easy answers either, as the Zn L2,3 edge is a delayed edge starting at 1040 eV and ending around 1150 eV with another little dip at 1207 eV, and although the Na K edge is a sharp edge, it is at 1070eV, right in the middle of a huge background presented by the Zn delayed edge. Well, students need to learn from their mistakes, and there is a lot to learn here.
John Mardinly, ASU
-----Original Message----- X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] Sent: Thursday, April 21, 2011 3:53 PM To: John Mardinly
I completely agree with Warren's comments below. Just I need to point out that all these considerations hold true for analysis of flat bulk specimens (SEM and microprobe).
EDS analysis of thin specimens in the TEM is strongly dependent on sample thickness, geometry of the particle and position of the point of analysis with respect to the grid bars of the supporting TEM grid. Quantification and comparison of peak profile and intensity for low energy L lines with high energy K lines is meaningless unless proper absorption correction and thickness are taken into account.
Also when analysis is done of a particle close to the TEM grid bars secondary scattering and fluorescence from grid bar material changes the peak intensities ratios between low energy (below ~ 1.5 KeV) and higher energy peaks. Thus quantitative comparison of peak intensities among different family of lines cannot be used as a reliable factor in the TEM.
Krassimir.
On Apr 21, 2011, at 3:04 PM, wesaia-at-iastate.edu wrote:
} } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } I am basing my comments on the article citation provided by Jim Quinn. } http://pubs.acs.org/doi/suppl/10.1021/ja908521s/suppl_file/ja908521s_si_ 001.pdf } } WDS should give a more definitive identification I am in the SEM lab now and don't know off the top of my head how well the WDS resolves Na-K and Zn-L. I know it does very well with Mo-L and S-K. I could check when I am back in the probe lab. } } I doubt that different voltages would help much. It would alter the ratio between the lines at 9 keV and 1 keV, but it would not help to differentiate the Na and Zn portions of the peak near 1 keV. } } Some of the peak at 1 keV will be due to an Oxygen sum peak, but sum peaks will be only fractions of the main peaks and the O peak at 0.53 keV is much smaller than the peak at 1 keV, so the portion due to O contribution is probably negligible. } } Alan Nicholls suggested that the EDS spectrum in Fig S3-c is from an SEM while the spectrum in S5-d is from a TEM. That may be, but the images in part-a of the figures are both TEM images. The other graphics look the same. I would suppose the spectra are both from a TEM. } } I can live with the mislabeling in the figures, but the fact that the peak at 1 kV is higher in the sample that was not doped with Na than in the one doped with Na would normally lead me to conclude that there is more Na in the undoped sample than in the doped one. I would normally except the Zn L and K lines to have a fixed ratio. } } However, the background signal looks markedly different in the two spectra. Figure S3-c has background visible across the energy range. The O peak looks to be about 10% of the Zn-K peak. Figure S5-d shows very little background around 5 keV. The O peak is more than 50% of the Zn-K peak. Note that the ratio of O intensity to the intensity of the peak at 1 keV is roughly the same in both spectra. That tells me that the spectrum in S3-c was from an area facing more away from the EDS detector than the area used for S5-d. There is no definite difference in the peak at 1.0 keV due to the presence of Na. } } The plots in S3-d prove nothing. They are predominantly from the Zn-L line. } } Indeed, some serious revisions or retractions are in order. } } Warren S. } } -----Original Message----- } X-from: voyles-at-engr.wisc.edu [mailto:voyles-at-engr.wisc.edu] } Sent: Thursday, April 21, 2011 4:03 PM } To: wesaia-at-iastate.edu } Subject: [Microscopy] Re: Na in ZnO } } } } } ------------------------------------------------------------------------ ---- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ------------------------------------------------------------------------ ---- } } On 4/21/2011 3:55 PM, bozzola-at-siu.edu wrote: } } ------------------------------------------------------------------------ ---- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ------------------------------------------------------------------------ ---- } } } } Follow-up questions to Peter and Bill: } } } } 1. Would WDS give a definitive identification? } } } } 2. Would using a variety of kV's using EDS give a better identification? } } I'll add a third question: why is the Zn K to Zn L ratio so different in } the two EDS spectra in the supporting information? The ratio is much } greater than 1 for the first spectrum and much less than 1 for the } second spectrum. } } Best wishes, } Paul } } } } } } Thanks, } } } } John Bozzola } } } } ----------------------------------------------------- } } } } } } } } } Except B of course for the possibility of the doublet of Oxygen K } } } (0.523 x 2 = 1.046 KeV) B The sample is ZnO! } } } } } } Peter I. } } } } } } } Dear Krassimir, } } } } B B B B 1% is possible, but close to the limit of detectability. B I } } } } presume that the EDS system has an ultrathin window or no window. B As to } } } } the prudence, if the spectra show a bump at the right position, and there } } } } is no possibility of interference from another element, (I don't have } } } } access to a table of x-ray lines, but I know that Cu-L is somewhere near } } } } Na-K.) I see nothing wrong with reporting it as Na. B At one time I was } } } } looking for chlorinated hydrocarbons in clay samples, and I could see a } } } } small bump where the Cl should be in some of the grains. B I also saw the } } } } Ti-L alpha clearly and the Ti-L beta was the same size as the Cl and } } } } obviously must be there. B This gave me confidence that the Cl bump was } } } } also real. } } } } B B B B B B B B B B B B B B B B B B B B Yours, } } } } B B B B B B B B B B B B B B B B B B B B B B Bill } } } } } } } } } } } } } } } } X-from: B bozhilov-at-ucr.edu } } } } To: B B William.F.Tivol-at-aero.org } } } } Date: B 04/20/2011 01:02 PM } } } } Subject: B B B B [Microscopy] Na in ZnO } } } } } } } } } } } } } } } } A recent paper in JACS (do not want to provide citation intentionally) } } } } clams to have used EDS analysis in the TEM to determine presence of about } } } } 1% of Na in ZnO single crystals. Any comments on validity and prudence of } } } } such a claim? } } } } } } } } Krassimir Bozhilov } } } } } } } } } } } } } } } } } } } } } -- } } } Peter Ingram } } } Sr. Physicist } } } Adj. Professor of Pathology } } } Duke University Medical Center } } } PO Box 90319 } } } DURHAM B NC B 27708-0319 } } } } } } } -- } Paul Voyles } Materials Science and Engineering } University of Wisconsin, Madison } 1509 University Ave, Rm 223 } Madison, WI 53706-1595 } voice: (608) 265-6740 } fax: (608) 262-8353 } voyles-at-engr.wisc.edu } http://tem.msae.wisc.edu } } ==============================Original Headers============================== } 7, 26 -- From voyles-at-engr.wisc.edu Thu Apr 21 16:03:03 2011 } 7, 26 -- Received: from smtp-out.cae.wisc.edu (smtp-out.cae.wisc.edu [144.92.12.60]) } 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LL326T011724 } 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 } 7, 26 -- Received: from smtp.cae.wisc.edu (smtp.cae.wisc.edu [IPv6:2607:f388:1082:0:ac8d:e958:beb5:d993]) } 7, 26 -- by smtp-out.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL32kW001354 } 7, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) } 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 } 7, 26 -- Received: from [IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65] ([IPv6:2607:f388:1082:fff3:f00f:a0e3:261b:3a65]) } 7, 26 -- (authenticated bits=0) } 7, 26 -- by smtp.cae.wisc.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id p3LL31Hq005050 } 7, 26 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NOT) } 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:03:02 -0500 } 7, 26 -- X-Virus-Status: Clean } 7, 26 -- X-Virus-Scanned: clamav-milter 0.96.5 at smtp.cae.wisc.edu } 7, 26 -- Message-ID: {4DB09B8D.3040903-at-engr.wisc.edu} } 7, 26 -- Date: Thu, 21 Apr 2011 16:03:09 -0500 } 7, 26 -- From: Paul Voyles {voyles-at-engr.wisc.edu} } 7, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 } 7, 26 -- MIME-Version: 1.0 } 7, 26 -- To: Microscopy-at-microscopy.com } 7, 26 -- Subject: Re: [Microscopy] Na in ZnO } 7, 26 -- References: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} } 7, 26 -- In-Reply-To: {201104212055.p3LKtGbW029295-at-ns.microscopy.com} } 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 26 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 24, 31 -- From wesaia-at-iastate.edu Thu Apr 21 16:59:45 2011 } 24, 31 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) } 24, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LLxixI012420 } 24, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:59:44 -0500 } 24, 31 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702a.its.iastate.edu [10.10.87.140]) } 24, 31 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id p3LLxemT015607 } 24, 31 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 16:59:44 -0500 } 24, 31 -- Received: from EXITS711.its.iastate.edu ([10.10.87.143]) by } 24, 31 -- EXITS702A.its.iastate.edu ([10.10.87.140]) with mapi; Thu, 21 Apr 2011 } 24, 31 -- 16:59:42 -0500 } 24, 31 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} } 24, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 24, 31 -- Date: Thu, 21 Apr 2011 17:04:22 -0500 } 24, 31 -- Subject: RE: [Microscopy] Re: Na in ZnO } 24, 31 -- Thread-Topic: [Microscopy] Re: Na in ZnO } 24, 31 -- Thread-Index: AcwAZ45WC7HEjqL7QKuYM15TmJ8+/gABU70g } 24, 31 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F13580E1B30-at-EXITS711.its.iastate.edu} } 24, 31 -- References: {201104212103.p3LL3Onp012628-at-ns.microscopy.com} } 24, 31 -- In-Reply-To: {201104212103.p3LL3Onp012628-at-ns.microscopy.com} } 24, 31 -- Accept-Language: en-US } 24, 31 -- Content-Language: en-US } 24, 31 -- X-MS-Has-Attach: } 24, 31 -- X-MS-TNEF-Correlator: } 24, 31 -- acceptlanguage: en-US } 24, 31 -- Content-Type: text/plain; charset="iso-8859-1" } 24, 31 -- MIME-Version: 1.0 } 24, 31 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.4.21.214819 } 24, 31 -- X-ISUMailhub-test: Gauge=XXIII, Probability=23%, Report=' } 24, 31 -- RATWARE_RCVD_BONUS_SPC 2.0, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, DATE_TZ_NA 0, FROM_NAME_PHRASE 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_MEDIA_BODY 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __HIGHBITS 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' } 24, 31 -- Content-Transfer-Encoding: 8bit } 24, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LLxixI012420 } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 22 -- From bozhilov-at-ucr.edu Thu Apr 21 17:45:41 2011 11, 22 -- Received: from sententia.ucr.edu (sententia.ucr.edu [138.23.226.213]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LMjegJ012777 11, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 17:45:40 -0500 11, 22 -- Received: from cfamm2.ucr.edu (cfamm2.ucr.edu [138.23.103.15]) 11, 22 -- by sententia.ucr.edu (MOS 3.10.5-GA) 11, 22 -- with ESMTP id FGH04693 (AUTH bozhilov); 11, 22 -- Thu, 21 Apr 2011 15:45:38 -0700 (PDT) 11, 22 -- Content-Type: text/plain; charset=us-ascii 11, 22 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 22 -- Subject: Re: [Microscopy] Na in ZnO 11, 22 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu} 11, 22 -- In-Reply-To: {201104212204.p3LM4jJS021248-at-ns.microscopy.com} 11, 22 -- Date: Thu, 21 Apr 2011 15:45:38 -0700 11, 22 -- Message-Id: {3A23718C-947A-4AED-AC1B-B1E1C732B976-at-ucr.edu} 11, 22 -- References: {201104212204.p3LM4jJS021248-at-ns.microscopy.com} 11, 22 -- To: "Warren E [BIOTC] Straszheim" {wesaia-at-iastate.edu} , 11, 22 -- microscopy-at-microscopy.com 11, 22 -- X-Mailer: Apple Mail (2.1084) 11, 22 -- X-Junkmail-Status: score=0/50, host= 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LMjegJ012777 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 40 -- From John.Mardinly-at-asu.edu Thu Apr 21 18:26:41 2011 19, 40 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 19, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3LNQfQD029484 19, 40 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 18:26:41 -0500 19, 40 -- X-ASG-Debug-ID: 1303428391-5126472d0001-4CH8be 19, 40 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet8.asu.edu with ESMTP id gYSotyFayS7Py0hz (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Thu, 21 Apr 2011 16:26:31 -0700 (MST) 19, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 19, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 19, 40 -- X-ASG-Whitelist: Client 19, 40 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.207) by 19, 40 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 19, 40 -- 8.1.436.0; Thu, 21 Apr 2011 16:26:30 -0700 19, 40 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 19, 40 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 21 Apr 19, 40 -- 2011 16:26:30 -0700 19, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 40 -- Content-Class: urn:content-classes:message 19, 40 -- MIME-Version: 1.0 19, 40 -- Content-Type: text/plain; charset="us-ascii" 19, 40 -- Subject: RE: [Microscopy] Re: Na in ZnO 19, 40 -- Date: Thu, 21 Apr 2011 16:26:30 -0700 19, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: Na in ZnO 19, 40 -- Message-ID: {95FFD84122D92D4F913DE75095E9C42E0661034F-at-EX05.asurite.ad.asu.edu} 19, 40 -- In-Reply-To: {201104212253.p3LMrTI2028577-at-ns.microscopy.com} 19, 40 -- X-MS-Has-Attach: 19, 40 -- X-MS-TNEF-Correlator: 19, 40 -- Thread-Topic: [Microscopy] Re: Na in ZnO 19, 40 -- thread-index: AcwAdu/p4x60ZSriTFSqHXNADHCt3QAAwLDw 19, 40 -- References: {201104212253.p3LMrTI2028577-at-ns.microscopy.com} 19, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 19, 40 -- To: {bozhilov-at-ucr.edu} 19, 40 -- CC: {microscopy-at-microscopy.com} 19, 40 -- X-OriginalArrivalTime: 21 Apr 2011 23:26:30.0816 (UTC) FILETIME=[8B7DDA00:01CC007B] 19, 40 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 19, 40 -- X-Barracuda-Start-Time: 1303428391 19, 40 -- X-Barracuda-Encrypted: RC4-MD5 19, 40 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 19, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 19, 40 -- Content-Transfer-Encoding: 8bit 19, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3LNQfQD029484 ==============================End of - Headers==============================
All, Even with WDS there is some overlap but it can be corrected for quantitatively using an interference correction that includes the matrix effects relative to the standard used for the interference calibration since the absorption correction is large for Na Ka (see the Na ABSCOR below) in the presence of Zn and O. Note that the main overlap with Na Ka is with the Zn Lb1 line not the Zn La1 line.
From my CalcZAF program for Na in ZnO (assuming 50:50:1 ratio) using FFAST MACs and the Armstrong phi-rho-z corrections (at 20 keV):
SAMPLE: 1, ITERATIONS: 0, Z-BAR: 25.59163
ELEMENT ABSCOR FLUCOR ZEDCOR ZAFCOR STP-POW BKS-COR F(x)u Ec Eo/Ec MACs Zn ka .9971 1.0000 1.0512 1.0482 1.0764 .9766 .9867 9.6590 2.0706 37.1266 Na ka 5.3842 1.0000 .9175 4.9399 .8342 1.0998 .1426 1.0730 18.6393 6769.94 O ka 3.0800 .9978 .8383 2.5762 .7567 1.1078 .1931 .5317 37.6152 4838.20
ELEMENT K-RAW K-VALUE ELEMWT% OXIDWT% ATOMIC% FORMULA KILOVOL Zn ka .00000 .76214 79.885 ----- 49.505 .980 20.00 Na ka .00000 .00114 .562 ----- .990 .020 20.00 O ka .00000 .07590 19.553 ----- 49.505 .980 20.00 TOTAL: 100.000 ----- 100.000 1.980
Apologies for the formatting- but Nestor rules!
I do not have a measurement of Na in ZnO handy but I do for ZnTe (relative to pure Zn metal for Zn and Nepheline for Na at 20 keV). In ZnTe I get an apparent wt% concentration of .028 +/- 0.010 Na or 280 +/- 100 PPM but it's within 3 std devs of zero so the question is: is it real? I have no idea what the true concentration of Na in this ZnTe is, but it's supposed to be 5 "nines" pure material (metals basis of course).
Without an interference correction in WDS (using a TAP crystal), one gets an apparent wt% concentration of 1.68 +/- 0.009 Na, so an interference correction is important even in WDS.
I might look into this further since I have a ZnO single crystal standard in one of my mounts. john
==============================Original Headers============================== 10, 22 -- From donovan-at-uoregon.edu Thu Apr 21 19:49:23 2011 10, 22 -- Received: from smtp.uoregon.edu (oh-mserv3.uoregon.edu [128.223.142.146]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3M0nMqo014046 10, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 19:49:23 -0500 10, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 10, 22 -- (authenticated bits=0) 10, 22 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id p3M0nMeL025486 10, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 22 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2011 17:49:22 -0700 10, 22 -- Message-Id: {201104220049.p3M0nMeL025486-at-smtp.uoregon.edu} 10, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 22 -- Date: Thu, 21 Apr 2011 17:49:12 -0700 10, 22 -- To: microscopy-at-microscopy.com 10, 22 -- From: John Donovan {donovan-at-uoregon.edu} 10, 22 -- Subject: Re: [Microscopy] Na in ZnO 10, 22 -- Mime-Version: 1.0 10, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 10, 22 -- definitions=2011-04-21_07:2011-04-22,2011-04-21,1970-01-01 signatures=0 10, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=13 10, 22 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 10, 22 -- reason=mlx engine=6.0.2-1012030000 definitions=main-1104210167 ==============================End of - Headers==============================
The discussion that is going on is interesting and has digressed a bit to the resolution in WDS. Of course there will be difficulties in WDS, too, besides those of the instrument. One important one is that Na moves during analysis. Anyone who has ever looked at a glass with Na in it can attest to that. So there are also material issues. Especially since the samples are nanowires and WDS analysis in a microprobe would be more than a little tricky on one of them.
I'd like to make some comments about the original topic and a general statement. Paul Voyles touched on the topic of the ZnK to ZnL ratio. To do appropriate analysis in the TEM, you have to have a sufficiently thin sample that meets the thin film criteria. The principle concern of course is absorption. If the L to K ratio is not a constant and is smaller than a standard, then the sample is absorbing the lower energy X-rays and it is too thick. I published a little article on resolving severe peak overlaps in Microscopy Today a couple of years back that discusses the issue more fully. In one of their two spectra, there is a great deal of absorption of the low energy peaks. Never mentioned and never caught by reviewers!
The authors should have caught some of these points that contributors on the Listserver have pointed out, but certainly the reviewers should have. They should have caught the overlap issue in XEDS in the TEM as did Krassimir. Come on, 1% of a relatively light element with a severe peak overlap? No analysis information? No confidence value? No mention of the analysis technique at all? That, in itself, should have been caught. My concern is: why wasn't it caught? I'm afraid of the answer. It means people aren't learning, people aren't teaching, and they are making deductions from the data that they just don't understand. Is it because the instruments are too easy to use and take data that "looks" good? Or is it people are too rushed and pushed to publish and don't take the time to do it right and understand the data and the data collection process? I've seen it before. There are experts at institutions on instrumentation, but they aren't consulted or included on papers and the result is that garbage is published.
I had a bad day today and just decided to rant. Sorry about that.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-----Original Message----- X-from: bozhilov-at-ucr.edu [mailto:bozhilov-at-ucr.edu] Sent: Wednesday, April 20, 2011 1:01 PM To: swalck-at-southbaytech.com
A recent paper in JACS (do not want to provide citation intentionally) clams to have used EDS analysis in the TEM to determine presence of about 1% of Na in ZnO single crystals. Any comments on validity and prudence of such a claim?
Krassimir Bozhilov Central Facility for Advanced Microscopy and Microanalysis University of California, Riverside, CA 92521
phone 951 827 2998 fax 951 827 2489
==============================Original Headers============================== 7, 20 -- From bozhilov-at-ucr.edu Wed Apr 20 14:48:14 2011 7, 20 -- Received: from sententia.ucr.edu (sententia.ucr.edu [138.23.226.213]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3KJmEPW021315 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2011 14:48:14 -0500 7, 20 -- Received: from cfamm2.ucr.edu (cfamm2.ucr.edu [138.23.103.15]) 7, 20 -- by sententia.ucr.edu (MOS 3.10.5-GA) 7, 20 -- with ESMTP id FGD08574 (AUTH bozhilov) 7, 20 -- for {microscopy-at-microscopy.com} ; 7, 20 -- Wed, 20 Apr 2011 12:48:12 -0700 (PDT) 7, 20 -- From: Krassimir Bozhilov {bozhilov-at-ucr.edu} 7, 20 -- Content-Type: text/plain; charset=us-ascii 7, 20 -- Subject: Na in ZnO 7, 20 -- Date: Wed, 20 Apr 2011 12:48:11 -0700 7, 20 -- Message-Id: {1BEBCCA7-CF17-4069-BC5F-8C478CD7511B-at-ucr.edu} 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 7, 20 -- X-Mailer: Apple Mail (2.1084) 7, 20 -- X-Junkmail-Status: score=0/50, host= 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3KJmEPW021315 ==============================End of - Headers==============================
Hi Scott, You are correct of course that Na intensities can change over time, particularly in glasses. So even though the Na in Zn measurements I mentioned previously were in materials that did not contain Na, I still used a time dependent intensity (TDI) correction to extrapolate back to zero time. I pretty much do a TDI measurement routinely now because it doesn't hardly take any longer (a little software overhead is all) and you never know when it might be handy to have the intensity change over time well documented.
On the point about nano-wires and WDS I would just mention that one can utilize geometric models to account for particle and rod shapes smaller than the typical EPMA interaction volume (most of my EPMA work nowadays is characterizing compositions of thin films around 50 - 100 nm thick on electron opaque substrates), but it is helpful to have some knowledge of the specimen size and shape to make an accurate correction for such particle or rod geometries.
That also brings up the issue you mention of the Zn K and L lines. I would just add that given a choice I would probably perform WDS analysis using Zn La and Na Ka because the differences in the various corrections will be much smaller than the case for Zn Ka, since Zn La and Na Ka are very similar in energy (with regard to the absorption correction), though the presence of the Zn L edges are still problematic for the background correction. That is, the background intensity on the Na Ka side is significantly lower than on the Zn La side.
It's a non trivial problem for sure. john
At 06:48 PM 4/21/2011, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
He apparently left HTML mode on and the listserver bounced his message. ws ________________________________________ X-from: woody [woody-at-albe24.com] Sent: Thursday, April 21, 2011 8:26 PM To: wesaia-at-iastate.edu; MSA
I agree 100% with Warren. It is just about impossible using the "standardless" mode commonly available from the manufacturers. I would never trust the background subtraction and deconvolutions used to reliably detect Na in a Zn rich compound at 1% levels with "canned" standards.
Perhaps using the fully standardized mode with like-composition material for the standards and a well integrated, slowly collected (high filtering) spectrum, it may be possible but even then I would be very cautious.
I also agree that changing the excitation voltage will have no bearing on resolving the overlap issue.
My memory is a bit foggy, but when I had my Microspec WDS, I believe I could resolve the Na/Zn overlap. In general, how well depends on the how the WDS is setup (slit width, etc) and crystal quality.
Thank you in advance for assistance- this list is definitely worth more than my entire book collection!
I have a Technics Hummer II that does both plating and etching. Recently I started having issues with the voltage going to the target "Flashing" periodically. I traced the issue to a second HV cable which was just dangling in the cabinet of the coater, and it was near a ground line and was arcing with the ground.
My question is this: There seems to be two red cables coming out of the "Etch/Plate" switch box to the right of the coating chamber. One of them reaches the top of the chamber and can therefore connect to the target, the other can not. What is the second, shorter one for? For now I've got it wrapped in electrical tape to prevent the arcing issue, but I am curious about that. I've read the manual (An original!) cover to cover and found no reference to that at all- nor did I find a schematic or anything else that might be helpful. (Although it was interesting that when the manual was printed in 1976, an Au/Pd target cost $100.00- I doubt they'd still honor that price!)
This is more of a curiosity than anything else, the coater works fine now that I've got the second lead taped off, but I still want to know what it is for...
Hi to all!  We have a major problem with compressed air for our Tecnai G20 TEM. I’ll try to be as precise as possible but in the end I think that the answer should be quite standard.  I can remember that in the end of last year we had a discussion on the list about how important it was to make sure that the compressed air arriving at the TEM is dry to avoid wasting the valves. I took this message seriously and reviewed our own system but I found it to be well conceived since we had (1) a large pressure container which can collect a large volume of water between the compressor and the pipes. (2) a kind of filter after the container, although I admit I don’t really know its function. I guessed it was there to retain water.  The problem itself: about 2 weeks ago during a routine check we noticed that the small container located in the flow meter rack, which purpose is to collect the rest of condensed water from the pipes and which remained completely dry for 5 years was completely full with water. At that time it seemed that we came in time and the water did not overflow in the tubing to the microscope parts. The container was purged. Incidentally, several days later we had a planned power cutoff because of works on the main line and I decided to switch off the microscope completely. I was confident that with the power off there was no risk for the microscope until the problem of condensed water was solved by the engineers. However, several days later, with the system still switched off, we noticed again that the container in the flow meter rack was completely full, this time we could also see water in the transparent tubing going to the microscope. Some tubes are blue so we can’t see through. Now we blocked the compressed air entry to the flow meter rack with a valve but I need advices for the cleaning steps. The main question is: may I clean the tubes myself or is there a risk that I make a major mistake? And then, if I can do it myself, how should I proceed? What should I be aware of? I have nitrogen and SF6 tanks at my disposal to dry the tubes if it is necessary.  Many thanks in advance. I hope America can enjoy an early summer like we do in Europe.
Stephane
==============================Original Headers============================== 3, 34 -- From nizets2-at-yahoo.com Fri Apr 22 02:08:00 2011 3, 34 -- Received: from nm25-vm0.bullet.mail.sp2.yahoo.com (nm25-vm0.bullet.mail.sp2.yahoo.com [98.139.91.228]) 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p3M780q3028812 3, 34 -- for {microscopy-at-microscopy.com} ; Fri, 22 Apr 2011 02:08:00 -0500 3, 34 -- Received: from [98.139.91.66] by nm25.bullet.mail.sp2.yahoo.com with NNFMP; 22 Apr 2011 07:08:00 -0000 3, 34 -- Received: from [98.139.91.37] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 22 Apr 2011 07:08:00 -0000 3, 34 -- Received: from [127.0.0.1] by omp1037.mail.sp2.yahoo.com with NNFMP; 22 Apr 2011 07:08:00 -0000 3, 34 -- X-Yahoo-Newman-Property: ymail-3 3, 34 -- X-Yahoo-Newman-Id: 93980.79802.bm-at-omp1037.mail.sp2.yahoo.com 3, 34 -- Received: (qmail 65742 invoked by uid 60001); 22 Apr 2011 07:07:59 -0000 3, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1303456079; bh=QZNNBMpmRrnmvlro6ctmgxMrs8l7c5UngVcGZI44Csk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=cKGhhNYfaimqLcN3nuRP+sCMlkdQB7t02l73Z66xQVxr2bc27n/0v68xJ0e0GPEFAXZcjMjxqeONmoPJKLmAD7aR5L9pYIbw3FzGWMVql8SiLkRCXvQDpcnrmVOkkpsa+EyEQYjuLgwoSBOIjFc5yyeY1EjkenfUDZrHB815aFo= 3, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 34 -- s=s1024; d=yahoo.com; 3, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 3, 34 -- b=B3jffebdslBEj9yNB/8o1HZ9xs1PzonMr3r0tslhaEBZ657LS9UXOo3vnxe55cPu43oYop9eFgAcjjOnLYfIore6FNRS/yQwLVs+IadflbHWu+ObyXJO0S/f/yk+AkjGwMTGRcZw3vHAhLoT8HNDGMorW6ct3QGxk+eCXEEXGcs=; 3, 34 -- Message-ID: {653623.54769.qm-at-web110804.mail.gq1.yahoo.com} 3, 34 -- X-YMail-OSG: 4euVNJUVM1nBW949xmOGphOi_sbWtsuSU8WFoOLWPySTf7u 3, 34 -- bwuf9awL6iadHbO3_N0d_rVyZD9yoCJW9z1J7Zn6D2NCN2fJsqtRdqI8GIX5 3, 34 -- Rbx_YwGjMcM4N_G64cYMBRueg7NjGQSAsr9EdqQorrX6qP0c.uPDq09zGBqA 3, 34 -- 2N7WLh7cC66eDA2kdiQgJVPSIQJ3U9rHf28JUSv.BImwV6D5gnuoIED.Z0u5 3, 34 -- fLd7XQ0jPDkgqr6wqkgiuPBOgEcQOJNVEt1_t2hFIUNYmUpHX5O6Hf3c9sKU 3, 34 -- oRaaViE0VTet_XMge2HaB8g5cDCP5dQI.AeJVW9JjLT813AOWmGViKBNDIfK 3, 34 -- Z5fYhjxeo3oUojyfge9FsujkRGRWZtFhUG5j9sEriwczQ0inhydiQxGlSSDr 3, 34 -- O_L3zBj91LrP2_w-- 3, 34 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Fri, 22 Apr 2011 00:07:59 PDT 3, 34 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.110.299900 3, 34 -- Date: Fri, 22 Apr 2011 00:07:59 -0700 (PDT) 3, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 34 -- Subject: problem with compressed air 3, 34 -- To: microscopy-at-microscopy.com 3, 34 -- MIME-Version: 1.0 3, 34 -- Content-Type: text/plain; charset=utf-8 3, 34 -- Content-Transfer-Encoding: 8bit 3, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3M780q3028812 ==============================End of - Headers==============================
You say that you want to label a surface antigen right? In this case you may try to make the immuno reaction before the fixation, then the following steps can't disturb the immuno reaction.
Incidentally I remember an interesting finding of Prof Walther, you may want to check it out: http://www.ncbi.nlm.nih.gov/pubmed/18445157
Regards,
Stephane
Â
----- Original Message ---- X-from: "reinhard.rachel-at-biologie.uni-regensburg.de" {reinhard.rachel-at-biologie.uni-regensburg.de} To: nizets2-at-yahoo.com Sent: Mon, April 18, 2011 9:25:53 PM
} We are using high-pressure freezing along with freeze substitution to try } preserving the structure of Arabidopsis pollen. One of the challenges is } preserving the pollen kitt (tryphine) on the surface of the pollen which } mostly comprises of lipids and sterol-esters. We have attempted to use just
} paraformaldehyde (2%) and glutaraldehyde (0.2%) in the freeze substitution } fixative in the hopes that the proteinaceous components of the pollen kitt } would be sufficiently fixed to retain its structure after embedding but this
} has clearly shown to not be the case.Â
first, I do not have any experience with plant material, I have to admit. I can imagine that bio-material with lots of lipids and sterol esters is difficult to preserve. Whether the material is "sufficiently fixed to retain its structure after embedding", not only depends on the aldehydes but may also depend on the solvent, and the resin. You do not write which solvent is used, acetone, or ethanol, methanol, or something else? One of the tricky tasks is to find the best solvent for substitution, along with a good time schedule. Not easy. In addition, you don't know which embedding medium is to be used - which is usually infiltrated at -30, 0 C, or even room temp, but under these conditions, the liquid, monomer embedding medium is an organic solvent, which may / will extract some of the (hydrophobic) bio-material, although we don't like this to happen ...
} Our final option would be to try varying concentrations of osmium (which is
} known to fix lipid components) but we are very concerned about loss of } immunogenicity (which we will require from the protocol).Â
I do not trust OsO4 too much - I have seen too many cases where "solvent" + 0.5 (0.25) % Uac, plus aldehyde plus 2-5% Water (Yes!!) has been superior to adding OsO4 in any concentration. What I would try first: solvent + 0.5% Uranyl Acetate, aldehyde, (water).
} Does anyone have any experience with the use of osmium in low temperature } freeze substitution protocols where immunogenicity is adequately preserved?
I recall that those who have tried, reported some 0.0x or up to 0.x % OsO4 (x = 1 - 2), in acetone ... See above: try to omit OsO4 completely. Only use UAc. Worth a try. Alternatively: try to freeze-substitute with resin monomer, already present at very low temp. in the solvent. Epoxy was seen to act as fixative during freeze-substitution. see Matsko and M端ller 2005 JSB
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 10, 24 -- From reinhard.rachel-at-biologie.uni-regensburg.de Mon Apr 18 14:23:21 2011 10, 24 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 10, 24 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3IJNKx1010652 10, 24 -- Â Â Â for {Microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 14:23:20 -0500 10, 24 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 10, 24 -- Â Â Â by localhost (Postfix) with SMTP id 92C8B37C9 10, 24 -- Â Â Â for {Microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 21:23:17 +0200 (CEST) 10, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 10, 24 -- Â Â Â by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 75A9737C7 10, 24 -- Â Â Â for {Microscopy-at-microscopy.com} ; Mon, 18 Apr 2011 21:23:17 +0200 (CEST) 10, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 10, 24 -- Â Â Â with Novell_GroupWise; Mon, 18 Apr 2011 21:23:17 +0200 10, 24 -- Message-Id: {4DACABC3020000540000CC25-at-gwsmtp1.uni-regensburg.de} 10, 24 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 10, 24 -- Date: Mon, 18 Apr 2011 21:23:15 +0200 10, 24 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 10, 24 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 10, 24 -- Subject: Osmium, freeze substitution, and immonogenicity 10, 24 -- References: {201104181826.p3IIQPRS025938-at-ns.microscopy.com} 10, 24 -- In-Reply-To: {201104181826.p3IIQPRS025938-at-ns.microscopy.com} 10, 24 -- Mime-Version: 1.0 10, 24 -- Content-Type: text/plain; charset=UTF-8 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
Firstly I feel there is a misunderstanding in relation to the compressed air and moisture. Moisture, anywhere in the system, is a big problem. The compressed air when generated or contained in a moist zone will carry the moisture to the control valves which usually have aluminium bases. When an aluminium control valve becomes moist it will oxidise, which ultimately damages the valve and causes an air leek; hissing!
How to minimise the damage that your problem may cause? Firstly drain ALL of the compressed air containers, there should be a tap at the base of each.
Now it is down to ideas on cleaning the lines. If it was me I would disconnect the input line to the microscope and blow through the pipes with compressed air from another "dry" source or from as gas bottle (nitrogen would be fine). Cleaning the actuation valves without taking them all apart and blowing though is I think an impossible task.
Good luck buddy!
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
.
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: 22 April 2011 08:09 To: protrain-at-emcourses.com
Hi to all!  We have a major problem with compressed air for our Tecnai G20 TEM. I’ll try to be as precise as possible but in the end I think that the answer should be quite standard.  I can remember that in the end of last year we had a discussion on the list about how important it was to make sure that the compressed air arriving at the TEM is dry to avoid wasting the valves. I took this message seriously and reviewed our own system but I found it to be well conceived since we had (1) a large pressure container which can collect a large volume of water between the compressor and the pipes. (2) a kind of filter after the container, although I admit I don’t really know its function. I guessed it was there to retain water.  The problem itself: about 2 weeks ago during a routine check we noticed that the small container located in the flow meter rack, which purpose is to collect the rest of condensed water from the pipes and which remained completely dry for 5 years was completely full with water. At that time it seemed that we came in time and the water did not overflow in the tubing to the microscope parts. The container was purged. Incidentally, several days later we had a planned power cutoff because of works on the main line and I decided to switch off the microscope completely. I was confident that with the power off there was no risk for the microscope until the problem of condensed water was solved by the engineers. However, several days later, with the system still switched off, we noticed again that the container in the flow meter rack was completely full, this time we could also see water in the transparent tubing going to the microscope. Some tubes are blue so we can’t see through. Now we blocked the compressed air entry to the flow meter rack with a valve but I need advices for the cleaning steps. The main question is: may I clean the tubes myself or is there a risk that I make a major mistake? And then, if I can do it myself, how should I proceed? What should I be aware of? I have nitrogen and SF6 tanks at my disposal to dry the tubes if it is necessary.  Many thanks in advance. I hope America can enjoy an early summer like we do in Europe.
Stephane
==============================Original Headers============================== 3, 34 -- From nizets2-at-yahoo.com Fri Apr 22 02:08:00 2011 3, 34 -- Received: from nm25-vm0.bullet.mail.sp2.yahoo.com (nm25-vm0.bullet.mail.sp2.yahoo.com [98.139.91.228]) 3, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p3M780q3028812 3, 34 -- for {microscopy-at-microscopy.com} ; Fri, 22 Apr 2011 02:08:00 -0500 3, 34 -- Received: from [98.139.91.66] by nm25.bullet.mail.sp2.yahoo.com with NNFMP; 22 Apr 2011 07:08:00 -0000 3, 34 -- Received: from [98.139.91.37] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 22 Apr 2011 07:08:00 -0000 3, 34 -- Received: from [127.0.0.1] by omp1037.mail.sp2.yahoo.com with NNFMP; 22 Apr 2011 07:08:00 -0000 3, 34 -- X-Yahoo-Newman-Property: ymail-3 3, 34 -- X-Yahoo-Newman-Id: 93980.79802.bm-at-omp1037.mail.sp2.yahoo.com 3, 34 -- Received: (qmail 65742 invoked by uid 60001); 22 Apr 2011 07:07:59 -0000 3, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1303456079; bh=QZNNBMpmRrnmvlro6ctmgxMrs8l7c5UngVcGZI44Csk=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=cKGhhNYfaimqLcN3nuRP+sCMlkdQB7t02l73Z66xQVxr2bc27n/0v68xJ0e0GPEFAXZcjMjxqeONmoPJKLmAD7aR5L9pYIbw3FzGWMVql8SiLkRCXvQDpcnrmVOkkpsa+EyEQYjuLgwoSBOIjFc5yyeY1EjkenfUDZrHB815aFo= 3, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 34 -- s=s1024; d=yahoo.com; 3, 34 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 3, 34 -- b=B3jffebdslBEj9yNB/8o1HZ9xs1PzonMr3r0tslhaEBZ657LS9UXOo3vnxe55cPu43oYop9eFgAcjjOnLYfIore6FNRS/yQwLVs+IadflbHWu+ObyXJO0S/f/yk+AkjGwMTGRcZw3vHAhLoT8HNDGMorW6ct3QGxk+eCXEEXGcs=; 3, 34 -- Message-ID: {653623.54769.qm-at-web110804.mail.gq1.yahoo.com} 3, 34 -- X-YMail-OSG: 4euVNJUVM1nBW949xmOGphOi_sbWtsuSU8WFoOLWPySTf7u 3, 34 -- bwuf9awL6iadHbO3_N0d_rVyZD9yoCJW9z1J7Zn6D2NCN2fJsqtRdqI8GIX5 3, 34 -- Rbx_YwGjMcM4N_G64cYMBRueg7NjGQSAsr9EdqQorrX6qP0c.uPDq09zGBqA 3, 34 -- 2N7WLh7cC66eDA2kdiQgJVPSIQJ3U9rHf28JUSv.BImwV6D5gnuoIED.Z0u5 3, 34 -- fLd7XQ0jPDkgqr6wqkgiuPBOgEcQOJNVEt1_t2hFIUNYmUpHX5O6Hf3c9sKU 3, 34 -- oRaaViE0VTet_XMge2HaB8g5cDCP5dQI.AeJVW9JjLT813AOWmGViKBNDIfK 3, 34 -- Z5fYhjxeo3oUojyfge9FsujkRGRWZtFhUG5j9sEriwczQ0inhydiQxGlSSDr 3, 34 -- O_L3zBj91LrP2_w-- 3, 34 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Fri, 22 Apr 2011 00:07:59 PDT 3, 34 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.110.299900 3, 34 -- Date: Fri, 22 Apr 2011 00:07:59 -0700 (PDT) 3, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 34 -- Subject: problem with compressed air 3, 34 -- To: microscopy-at-microscopy.com 3, 34 -- MIME-Version: 1.0 3, 34 -- Content-Type: text/plain; charset=utf-8 3, 34 -- Content-Transfer-Encoding: 8bit 3, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3M780q3028812 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From protrain-at-emcourses.com Fri Apr 22 03:30:10 2011 20, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3M8U9QU005999 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 22 Apr 2011 03:30:10 -0500 20, 28 -- Received: from 5ad38803.bb.sky.com ([90.211.136.3]:50509 helo=ProtrainPC) 20, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.74 #0) 20, 28 -- (envelope-from {protrain-at-emcourses.com} ) 20, 28 -- id 1QDBkb-0006GG-5a by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Fri, 22 Apr 2011 10:30:06 +0200 20, 28 -- Reply-To: {protrain-at-emcourses.com} 20, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 20, 28 -- To: {nizets2-at-yahoo.com} 20, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 20, 28 -- References: {201104220708.p3M78VvM030445-at-ns.microscopy.com} 20, 28 -- In-Reply-To: {201104220708.p3M78VvM030445-at-ns.microscopy.com} 20, 28 -- Subject: RE: [Microscopy] problem with compressed air 20, 28 -- Date: Fri, 22 Apr 2011 09:29:59 +0100 20, 28 -- Organization: Protrain 20, 28 -- Message-ID: {00ca01cc00c7$7a6d3110$6f479330$-at-com} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="utf-8" 20, 28 -- X-Mailer: Microsoft Office Outlook 12.0 20, 28 -- Thread-Index: AcwAvCKGGtUpruKPT3qcJdZ/EVrdRwABLi1g 20, 28 -- Content-Language: en-gb 20, 28 -- X-Scan-Signature: 3b0414da2beadeab703fc9dfd9e5588c{183}} 20, 28 -- X-Trace: smtp01.dial-up.net 1QDBkb-0006GG-5a 36854178ff16c7a4c91e3e972a4d3c18 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3M8U9QU005999 ==============================End of - Headers==============================
To all students (or those of you who might have students) attending the 2011 M&M meeting in Nashville, TN, please consider the student bursary program offered by MSA. The purpose of these bursaries is to encourage students to attend the annual MSA/MAS Microscopy and Microanalysis meeting, where they can meet and interact with the established microscopy community while defraying some meeting costs.
Students work for 20 hours (or up to 40 hours) during the meeting and pre-meeting events and are paid $10 an hour. The jobs involve such things as providing support in the different symposia (helping with audio-visual needs, maintaining an attendance count, and helping speakers set up for their presentation), staffing the volunteer office, monitoring use of the Internet Café, and helping with vendor tutorials and poster set-up. Payment is given as a check at the end of the meetings or when the student leaves Nashville.
Once the task list has been finalized, each bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up *working* sessions they would attend anyway. There is an added bonus of $10 cash meal allotment for each morning and/or afternoon worked and a meeting shirt.
If anyone would like to participate in the bursary program, please check the *I wish to apply for a student bursary* box in section 2 of the registration form. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid the registration fee.
For those *non-students* volunteers are needed to help with the above mentioned meeting activities as well. Although not paid on an hourly basis as the student bursaries, volunteers do receive the same cash allotment for meals and shirt. Plus they also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.
If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact:
Amanda Lawrence Institute for Imaging and Analytical Technologies Mississippi State University 662-325-3019 alawrence-at-i2at.msstate.edu
==============================Original Headers============================== 11, 21 -- From ALawrence-at-entomology.msstate.edu Mon Apr 25 10:49:22 2011 11, 21 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3PFnMRK026313 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 25 Apr 2011 10:49:22 -0500 11, 21 -- Received: from mailhost.groupwise.msstate.edu (mailhost.groupwise.msstate.edu [130.18.2.197]) 11, 21 -- by catalpa.its.msstate.edu (8.13.8/8.13.8) with ESMTP id p3PFnLUv008710 11, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=OK) 11, 21 -- for {microscopy-at-microscopy.com} ; Mon, 25 Apr 2011 10:49:21 -0500 11, 21 -- X-Sender: {} 11, 21 -- Received: from GATEWAY-MTA by mailhost.groupwise.msstate.edu 11, 21 -- with Novell_GroupWise; Mon, 25 Apr 2011 10:49:21 -0500 11, 21 -- Message-Id: {4DB551AE020000E60009858C-at-mailhost.groupwise.msstate.edu} 11, 21 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 11, 21 -- Date: Mon, 25 Apr 2011 10:49:18 -0500 11, 21 -- From: "Amanda Lawrence" {ALawrence-at-entomology.msstate.edu} 11, 21 -- To: {microscopy-at-microscopy.com} 11, 21 -- Subject: Microscopy and Microanalysis 2011 Nashville, TN 11, 21 -- Mime-Version: 1.0 11, 21 -- Content-Type: text/plain; charset=ISO-8859-15 11, 21 -- Content-Transfer-Encoding: 8bit 11, 21 -- Content-Disposition: inline ==============================End of - Headers==============================
A colleague has a functional Philips 300 TEM that he needs to have removed within the next two weeks. He is willing to give it away, all you have to do is remove it. It is located at Providence College in Providence, RI.
Please contact him directly as he is not on this list. Joseph A DeGiorgis, PhD Biology Department Providence College Providence, RI 02 918-0001 jdegiorg-at-providence.edu 508-292-4605 (Cell)
Thanks, Louie
--
Louis Kerr lkerr-at-mbl.edu
Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
VISIT OUR WEB SITES: www.mbl.edu www.courses.mbl.edu
==============================Original Headers============================== 8, 32 -- From lkerr-at-mbl.edu Mon Apr 25 13:13:59 2011 8, 32 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3PIDxod015768 8, 32 -- for {Microscopy-at-Microscopy.Com} ; Mon, 25 Apr 2011 13:13:59 -0500 8, 32 -- Received: from mailfrontier.mbl.edu (127.0.0.1) by mailfrontier.mbl.edu (MlfMTA v3.2r9) id hmmsue0171s6 for {Microscopy-at-Microscopy.Com} ; Mon, 25 Apr 2011 14:13:58 -0400 (envelope-from {lkerr-at-mbl.edu} ) 8, 32 -- Received: from mail.mbl.edu ([128.128.172.47]) 8, 32 -- by mailfrontier.mbl.edu (SonicWALL 7.2.1.2841) 8, 32 -- with ESMTP; Mon, 25 Apr 2011 14:13:58 -0400 8, 32 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 8, 32 -- by mail.mbl.edu (Postfix) with ESMTP id DAD681824002 8, 32 -- for {Microscopy-at-Microscopy.Com} ; Mon, 25 Apr 2011 14:13:58 -0400 (EDT) 8, 32 -- X-Virus-Scanned: amavisd-new at mbl.edu 8, 32 -- Received: from mail.mbl.edu ([127.0.0.1]) 8, 32 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10024) 8, 32 -- with ESMTP id t9jjJJEVJ7B7 for {Microscopy-at-Microscopy.Com} ; 8, 32 -- Mon, 25 Apr 2011 14:13:58 -0400 (EDT) 8, 32 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) 8, 32 -- by mail.mbl.edu (Postfix) with ESMTP id 8044E173007B 8, 32 -- for {Microscopy-at-Microscopy.Com} ; Mon, 25 Apr 2011 14:13:58 -0400 (EDT) 8, 32 -- Date: Mon, 25 Apr 2011 14:13:58 -0400 (EDT) 8, 32 -- From: Louis Kerr {lkerr-at-mbl.edu} 8, 32 -- To: Microscopy {Microscopy-at-Microscopy.Com} 8, 32 -- Message-ID: {507730082.286030.1303755238483.JavaMail.root-at-mail.mbl.edu} 8, 32 -- In-Reply-To: {71278355.285982.1303755104209.JavaMail.root-at-mail.mbl.edu} 8, 32 -- Subject: Philips 300 TEM 8, 32 -- MIME-Version: 1.0 8, 32 -- Content-Type: text/plain; charset=utf-8 8, 32 -- Content-Transfer-Encoding: 7bit 8, 32 -- X-Originating-IP: [10.10.5.235] 8, 32 -- X-Mailer: Zimbra 6.0.8_GA_2685 (ZimbraWebClient - FF3.0 (Mac)/6.0.8_GA_2685) 8, 32 -- X-Mlf-Version: 7.2.1.2841 8, 32 -- X-Mlf-UniqueId: o201104251813580097229 ==============================End of - Headers==============================
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
I have a request for imaging magnetic nanoparticles in TEM. The particles are currently in dry powder form. They are easily attracted to a small permanent magnet. My initial reaction is to refuse the request for fear of coating the objective lens with magnetic particles.
However, perhaps someone has successfully imaged such and can advise a safe method for introducing them into the TEM.
Cheers Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 7, 21 -- From raristau-at-ims.uconn.edu Tue Apr 26 09:41:11 2011 7, 21 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QEfBHn009243 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 09:41:11 -0500 7, 21 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu [137.99.20.234]) 7, 21 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 51D6A2D143 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:41:11 -0400 (EDT) 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 7, 21 -- Date: Tue, 26 Apr 2011 10:41:08 -0400 7, 21 -- Subject: TEM of magnetic particles 7, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 7, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C9DC51C4.28BE%raristau-at-ims.uconn.edu} 7, 21 -- Thread-Topic: TEM of magnetic particles 7, 21 -- Thread-Index: AcwEH/p+OT+9M3ATEeC/vAAbY55CVA== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
You are right in a sense. The biggest problem with magnetic particles is that they are magnetic. My biggest concern would be could you even get an image that did not exhibit serious aberration as a result of the magnetic field, small as it is, interacting with the electrons in the beam. That may well be the biggest problem.
paul
-- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 6, 21 -- From paul_hazelton-at-umanitoba.ca Tue Apr 26 10:14:56 2011 6, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QFEuZO026030 6, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:14:56 -0500 6, 21 -- Received: from [140.193.25.116] (basic116.medmb.umanitoba.ca [140.193.25.116]) 6, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) 6, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p3QFEsbg028535 6, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); 6, 21 -- Tue, 26 Apr 2011 10:14:54 -0500 (CDT) 6, 21 -- Message-ID: {4DB6E16D.7030305-at-umanitoba.ca} 6, 21 -- Date: Tue, 26 Apr 2011 10:14:53 -0500 6, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 6, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.15) Gecko/20110303 Thunderbird/3.1.9 6, 21 -- MIME-Version: 1.0 6, 21 -- To: raristau-at-ims.uconn.edu, Microscopy Listserver {microscopy-at-microscopy.com} 6, 21 -- Subject: Re: [Microscopy] TEM of magnetic particles 6, 21 -- References: {201104261442.p3QEgv02011736-at-ns.microscopy.com} 6, 21 -- In-Reply-To: {201104261442.p3QEgv02011736-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
no problem with magnetic nanoparticles - this is one of my specialities, and I do it all the time with no problem at all. Just take care that they are dispersed, by creating a suspension in water, shaking all particles apart by a supersonic bath andd then drip a droplet on a holey carbon grid, before they re-coagulate. The smaller the individual particle, the less the magnetic force acting on it. The force is proportional to the integral over the particle's volume taken over its magnetization times the gradient of the magnetic field. (You may want to check http://en.wikipedia.org/wiki/Magnetic_force_microscope). The gradient is usually small, although the total field is around 1-2 Tesla for a 300 kV microscope, as is the volume of the specimen. Thus the total force is small. Care should be taken for extremely high-magnetic moment particles above a certain size. I also recommend to switch off the lenses (objective is most important) during specimen insertion, as the stray fields at the vacuum transfer port may be rather large.
Good luck - Joe
Prof. Dr. Josef Zweck - Elektronenmikroskopie Fakultät für Physik der Universität Regensburg D - 93040 Regensburg Fon: ++49 (0)941 943 2590 Fax: ++49 (0)941 943 4544 http://www-elektronenmikroskopie.uni-regensburg.de/ *** next upcoming EM conference: http://www.mc2011.de/ in Kiel/Germany from Aug. 28 - Sept. 2 *** Deutsche Ges. f. Elektronenmikroskopie: www.dge-homepage.de *** see also the European Microscopy Society's homepage *** http://www.eurmicsoc.org/
} } } {raristau-at-ims.uconn.edu} schrieb am 26.4.2011 um 16.51 Uhr in Nachricht {201104261451.p3QEp3YQ024669-at-ns.microscopy.com} :
} } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers } } I have a request for imaging magnetic nanoparticles in TEM. The particles } are currently in dry powder form. They are easily attracted to a small } permanent magnet. My initial reaction is to refuse the request for fear of } coating the objective lens with magnetic particles. } } However, perhaps someone has successfully imaged such and can advise a safe } method for introducing them into the TEM. } } Cheers } Roger } } Roger A. Ristau, PhD } Electron Microscopy Specialist } Institute of Materials Science } 97 North Eagleville Road } University of Connecticut } Storrs, CT 06269 } vox: 860-486-5453 } fax: 860-486-4745 } } } } } ==============================Original Headers============================== } 7, 21 -- From raristau-at-ims.uconn.edu Tue Apr 26 09:41:11 2011 } 7, 21 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu } [137.99.25.234]) } 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3QEfBHn009243 } 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 09:41:11 -0500 } 7, 21 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu } [137.99.20.234]) } 7, 21 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 51D6A2D143 } 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:41:11 -0400 (EDT) } 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 7, 21 -- Date: Tue, 26 Apr 2011 10:41:08 -0400 } 7, 21 -- Subject: TEM of magnetic particles } 7, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } 7, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } 7, 21 -- Message-ID: {C9DC51C4.28BE%raristau-at-ims.uconn.edu} } 7, 21 -- Thread-Topic: TEM of magnetic particles } 7, 21 -- Thread-Index: AcwEH/p+OT+9M3ATEeC/vAAbY55CVA== } 7, 21 -- Mime-version: 1.0 } 7, 21 -- Content-type: text/plain; } 7, 21 -- charset="US-ASCII" } 7, 21 -- Content-transfer-encoding: 7bit } 7, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 } 7, 21 -- X-Virus-Status: Clean } ==============================End of - Headers==============================
==============================Original Headers============================== 11, 25 -- From Josef.Zweck-at-physik.uni-regensburg.de Tue Apr 26 10:16:23 2011 11, 25 -- Received: from rrzmta5.uni-regensburg.de (rrzmta5.uni-regensburg.de [194.94.155.56]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QFGMgw029362 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:16:23 -0500 11, 25 -- Received: from rrzmta5.uni-regensburg.de (localhost [127.0.0.1]) 11, 25 -- by localhost (Postfix) with SMTP id 512BF3750 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 17:16:21 +0200 (CEST) 11, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 11, 25 -- by rrzmta5.uni-regensburg.de (Postfix) with ESMTP id 2C81B374D 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 17:16:20 +0200 (CEST) 11, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 11, 25 -- with Novell_GroupWise; Tue, 26 Apr 2011 17:16:21 +0200 11, 25 -- Message-Id: {4DB6FDE20200004000012600-at-gwsmtp1.uni-regensburg.de} 11, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 11, 25 -- Date: Tue, 26 Apr 2011 17:16:18 +0200 11, 25 -- From: "Josef Zweck" {Josef.Zweck-at-physik.uni-regensburg.de} 11, 25 -- To: {raristau-at-ims.uconn.edu} 11, 25 -- Cc: "Listserver Microscopy" {microscopy-at-microscopy.com} 11, 25 -- Subject: Antw: [Microscopy] TEM of magnetic particles 11, 25 -- References: {201104261451.p3QEp3YQ024669-at-ns.microscopy.com} 11, 25 -- In-Reply-To: {201104261451.p3QEp3YQ024669-at-ns.microscopy.com} 11, 25 -- Mime-Version: 1.0 11, 25 -- Content-Type: text/plain; charset=ISO-8859-15 11, 25 -- Content-Transfer-Encoding: 8bit 11, 25 -- Content-Disposition: inline ==============================End of - Headers==============================
Should you find a way of mounting the particles in safety may I help on the observation side?
With any magnetic material it is useful to reduce the level of the magnetic field in the objective lens. This is easily done using the eucentric stage to raise the specimen within the pole piece. With a "normal" specimen adjust the eucentric system so that as you change the Z prime you need to turn the objective lens anti clockwise. There are a few millimetres available to you and this will have quite an effect upon the lens field.
Remember your magnifications will be lower and your contrast will be higher. The magnification change due to the weaker lens and the contrast due to increasing the specimen to aperture distance and increasing aberrations.
Whilst I rarely carry out this action in this direction but often obtain more than the manufacturer claims in resolution by working the Z prime to lower the specimen within the lens, increasing the lens field and reducing its aberrations.
Good luck
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] Sent: 26 April 2011 15:42 To: protrain-at-emcourses.com
Dear Listers
I have a request for imaging magnetic nanoparticles in TEM. The particles are currently in dry powder form. They are easily attracted to a small permanent magnet. My initial reaction is to refuse the request for fear of coating the objective lens with magnetic particles.
However, perhaps someone has successfully imaged such and can advise a safe method for introducing them into the TEM.
Cheers Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 7, 21 -- From raristau-at-ims.uconn.edu Tue Apr 26 09:41:11 2011 7, 21 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QEfBHn009243 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 09:41:11 -0500 7, 21 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu [137.99.20.234]) 7, 21 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 51D6A2D143 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:41:11 -0400 (EDT) 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 7, 21 -- Date: Tue, 26 Apr 2011 10:41:08 -0400 7, 21 -- Subject: TEM of magnetic particles 7, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 7, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C9DC51C4.28BE%raristau-at-ims.uconn.edu} 7, 21 -- Thread-Topic: TEM of magnetic particles 7, 21 -- Thread-Index: AcwEH/p+OT+9M3ATEeC/vAAbY55CVA== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
All, I didn't chime in on the original question, but I would like to add my "2 cents" as they say on the general issue of compressed gases.
When we designed our new microscopy facility we found a large number of instruments using a large number of compressed air cylinders for valve control and N2 cylinders for vent gas. We then did a cost analysis and found that we could replace all this cylinder usage by a cheaper and easier means.
For the compressed air cylinders we designed an air drying system which utilized already clean and compressed dry air from the chemistry building to which we added two large capacity moisture dryers in parallel (so one could be changed while using the other) which happens about every three years or so and also a final activated carbon filter to remove hydrocarbons and a particle filter which gives us extremely dry, HC free compressed air at 110 psi. This compressed air is supplied to each instrument's gallery section to control instrument valves and the air moisture is monitored and connected to an alarm system.
For N2 use this dry clean air is supplied to a analytical grade N2 generation system (from Perkin Elmer) designed for ICP-MS systems so the purity is 99.996% or better with an N2 generation rate of 12 liters per minutes. The unit cost around $15K and uses a membrane filter that costs about $100 every 3 years to replace. This N2 gas is also supplied to each instrument's gallery section for use in venting.
With these two systems we can supply around 16 different analytical instruments and never buy/rent/haul or connect an air or N2 compressed gas cylinder again. I highly recommend this type of system for both low cost and ease of use. I have to admit- I never liked hauling those cylinders around very much.
The only compressed gases we still buy are a few P-10 and Ar cylinders which are used at very low rates and changed every 6 months to one year or so. The only caveat I would add is that if you hire contractors to plumb such a system in your lab you should specify "medical grade" plumbing which means they don't use any fluxes which could contaminate the system. One can also use Silphos solder while flowing N2 gas through the copper (used in refrigeration systems) which is also a fluxless method to prevent flux or scale formation inside the tubing. john
At 01:13 AM 4/26/2011, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
When I was at Western Digital, the SEMs would periodically need to have the final pole piece cleaned of magnetic particles, and these samples were solid sputtered films with obvious no loose particles and sub 10nm grain size, not powders. Be very careful with loose particles.
A. John Mardinly, ASU
-----Original Message----- X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] Sent: Tuesday, April 26, 2011 7:51 AM To: John Mardinly
Dear Listers
I have a request for imaging magnetic nanoparticles in TEM. The particles are currently in dry powder form. They are easily attracted to a small permanent magnet. My initial reaction is to refuse the request for fear of coating the objective lens with magnetic particles.
However, perhaps someone has successfully imaged such and can advise a safe method for introducing them into the TEM.
Cheers Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 7, 21 -- From raristau-at-ims.uconn.edu Tue Apr 26 09:41:11 2011 7, 21 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QEfBHn009243 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 09:41:11 -0500 7, 21 -- Received: from [137.99.20.234] (d20h234.public.uconn.edu [137.99.20.234]) 7, 21 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id 51D6A2D143 7, 21 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:41:11 -0400 (EDT) 7, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 7, 21 -- Date: Tue, 26 Apr 2011 10:41:08 -0400 7, 21 -- Subject: TEM of magnetic particles 7, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 7, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 7, 21 -- Message-ID: {C9DC51C4.28BE%raristau-at-ims.uconn.edu} 7, 21 -- Thread-Topic: TEM of magnetic particles 7, 21 -- Thread-Index: AcwEH/p+OT+9M3ATEeC/vAAbY55CVA== 7, 21 -- Mime-version: 1.0 7, 21 -- Content-type: text/plain; 7, 21 -- charset="US-ASCII" 7, 21 -- Content-transfer-encoding: 7bit 7, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 7, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 15, 39 -- From John.Mardinly-at-asu.edu Tue Apr 26 11:59:21 2011 15, 39 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 15, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QGxLCP028358 15, 39 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 11:59:21 -0500 15, 39 -- X-ASG-Debug-ID: 1303837124-7dc96f450005-4CH8be 15, 39 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet7.asu.edu with ESMTP id 3HG2XGasbNLnjSsd (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Tue, 26 Apr 2011 09:59:01 -0700 (MST) 15, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 15, 39 -- X-ASG-Whitelist: Client 15, 39 -- Received: from EX03.asurite.ad.asu.edu (129.219.12.197) by 15, 39 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 15, 39 -- 8.1.436.0; Tue, 26 Apr 2011 09:58:46 -0700 15, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 15, 39 -- EX03.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Tue, 26 Apr 15, 39 -- 2011 09:58:34 -0700 15, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 39 -- Content-Class: urn:content-classes:message 15, 39 -- MIME-Version: 1.0 15, 39 -- Content-Type: text/plain; charset="us-ascii" 15, 39 -- Subject: RE: [Microscopy] TEM of magnetic particles 15, 39 -- Date: Tue, 26 Apr 2011 09:58:33 -0700 15, 39 -- X-ASG-Orig-Subj: RE: [Microscopy] TEM of magnetic particles 15, 39 -- Message-ID: {95FFD84122D92D4F913DE75095E9C42E0661035D-at-EX05.asurite.ad.asu.edu} 15, 39 -- In-Reply-To: {201104261451.p3QEpHIa025166-at-ns.microscopy.com} 15, 39 -- X-MS-Has-Attach: 15, 39 -- X-MS-TNEF-Correlator: 15, 39 -- Thread-Topic: [Microscopy] TEM of magnetic particles 15, 39 -- thread-index: AcwEIWcr8tYX/9KESBqgU3L4GbENVwAETSkw 15, 39 -- References: {201104261451.p3QEpHIa025166-at-ns.microscopy.com} 15, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} 15, 39 -- To: {raristau-at-ims.uconn.edu} 15, 39 -- CC: {microscopy-at-microscopy.com} 15, 39 -- X-OriginalArrivalTime: 26 Apr 2011 16:58:34.0122 (UTC) FILETIME=[2D90F6A0:01CC0433] 15, 39 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 15, 39 -- X-Barracuda-Start-Time: 1303837141 15, 39 -- X-Barracuda-Encrypted: RC4-MD5 15, 39 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 15, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu 15, 39 -- Content-Transfer-Encoding: 8bit 15, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3QGxLCP028358 ==============================End of - Headers==============================
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
My old advisor at Michigan, Wil Bigelow, had a unique and cost effective method of capturing ultra high purity nitrogen gas for venting microscopes. There was an added bonus in that no regulator was required, and it could never overpressurize the chamber and damage ultrathin detector windows. The cost? Under $20! How? He would put a rubber stopper in the EDX dewar with a tube in it for attaching a surgical rubber hose that ran to an extra large beach ball. That hose was teed to another hose that ran to the microscope vent inlet. A small slit in the surgical rubber hose provided over-pressure protection. Boil-off from the EDX dewar would fill the beach ball, and that nitrogen would be used to vent the microscope. The only disadvantage, is that some EDX systems have an alarm for sensing low LN2 levels that goes through the fill opening, and that would be difficult to accommodate. I might add that it made the lab look a LOT more cheery and colorful, which was a nice thing to have during the dreary Michigan winters.
A. John Mardinly, ASU
-----Original Message----- X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu] Sent: Tuesday, April 26, 2011 8:48 AM To: John Mardinly
All, I didn't chime in on the original question, but I would like to add my "2 cents" as they say on the general issue of compressed gases.
When we designed our new microscopy facility we found a large number of instruments using a large number of compressed air cylinders for valve control and N2 cylinders for vent gas. We then did a cost analysis and found that we could replace all this cylinder usage by a cheaper and easier means.
For the compressed air cylinders we designed an air drying system which utilized already clean and compressed dry air from the chemistry building to which we added two large capacity moisture dryers in parallel (so one could be changed while using the other) which happens about every three years or so and also a final activated carbon filter to remove hydrocarbons and a particle filter which gives us extremely dry, HC free compressed air at 110 psi. This compressed air is supplied to each instrument's gallery section to control instrument valves and the air moisture is monitored and connected to an alarm system.
For N2 use this dry clean air is supplied to a analytical grade N2 generation system (from Perkin Elmer) designed for ICP-MS systems so the purity is 99.996% or better with an N2 generation rate of 12 liters per minutes. The unit cost around $15K and uses a membrane filter that costs about $100 every 3 years to replace. This N2 gas is also supplied to each instrument's gallery section for use in venting.
With these two systems we can supply around 16 different analytical instruments and never buy/rent/haul or connect an air or N2 compressed gas cylinder again. I highly recommend this type of system for both low cost and ease of use. I have to admit- I never liked hauling those cylinders around very much.
The only compressed gases we still buy are a few P-10 and Ar cylinders which are used at very low rates and changed every 6 months to one year or so. The only caveat I would add is that if you hire contractors to plumb such a system in your lab you should specify "medical grade" plumbing which means they don't use any fluxes which could contaminate the system. One can also use Silphos solder while flowing N2 gas through the copper (used in refrigeration systems) which is also a fluxless method to prevent flux or scale formation inside the tubing. john
At 01:13 AM 4/26/2011, you wrote:
} ----------------------------------------------------------------------- ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
Each tank is typically 220 cubic feet and with a large room and open door, I wouldn't think that there is too big a risk. What I use in my shop is an oil-based compressor that gets filtered through a coalescing filter that I think is rated at .05 microns for oil mist. The filter has an indicator that goes from green to red if the efficiency drops too much. It's still green and has been in use for about 25 years and I've had no problems with contamination. I only use the Freon for leak detecting with a Varian Smart Gauge so I don't use much.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, April 26, 2011 2:13 PM To: kenconverse-at-qualityimages.biz
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
Ken; An "A" cylinder is 1.76 cubic feet internally, which at 3,000 psi is 200 atmospheres which should be more like 352 cubic feet of nitrogen gas.
A. John Mardinly, ASU
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Tuesday, April 26, 2011 11:49 AM To: John Mardinly
Each tank is typically 220 cubic feet and with a large room and open door, I wouldn't think that there is too big a risk. What I use in my shop is an oil-based compressor that gets filtered through a coalescing filter that I think is rated at .05 microns for oil mist. The filter has an indicator that goes from green to red if the efficiency drops too much. It's still green and has been in use for about 25 years and I've had no problems with contamination. I only use the Freon for leak detecting with a Varian Smart Gauge so I don't use much.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, April 26, 2011 2:13 PM To: kenconverse-at-qualityimages.biz
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
Hi John, In another life I often heard them referred to as 220 cubic foot cylinders. I've never seen one with more than 2600psi, but that does come out to 305 cubic feet which is a good deal more than 220. I stand corrected. Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 4:53 PM To: kenconverse-at-qualityimages.biz
Ken; An "A" cylinder is 1.76 cubic feet internally, which at 3,000 psi is 200 atmospheres which should be more like 352 cubic feet of nitrogen gas.
Sent: Tuesday, April 26, 2011 11:49 AM To: John Mardinly
Each tank is typically 220 cubic feet and with a large room and open door, I wouldn't think that there is too big a risk. What I use in my shop is an oil-based compressor that gets filtered through a coalescing filter that I think is rated at .05 microns for oil mist. The filter has an indicator that goes from green to red if the efficiency drops too much. It's still green and has been in use for about 25 years and I've had no problems with contamination. I only use the Freon for leak detecting with a Varian Smart Gauge so I don't use much.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, April 26, 2011 2:13 PM To: kenconverse-at-qualityimages.biz
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
In any case, a single bottle is not nearly the hazard that plumbed in nitrogen is.
A. John Mardinly, ASU
-----Original Message----- X-from: Ken Converse [mailto:kenconverse-at-qualityimages.biz] Sent: Tuesday, April 26, 2011 3:19 PM To: John Mardinly; MSA Listserver
Ken; An "A" cylinder is 1.76 cubic feet internally, which at 3,000 psi is 200 atmospheres which should be more like 352 cubic feet of nitrogen gas.
Sent: Tuesday, April 26, 2011 11:49 AM To: John Mardinly
Each tank is typically 220 cubic feet and with a large room and open door, I wouldn't think that there is too big a risk. What I use in my shop is an oil-based compressor that gets filtered through a coalescing filter that I think is rated at .05 microns for oil mist. The filter has an indicator that goes from green to red if the efficiency drops too much. It's still green and has been in use for about 25 years and I've had no problems with contamination. I only use the Freon for leak detecting with a Varian Smart Gauge so I don't use much.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, April 26, 2011 2:13 PM To: kenconverse-at-qualityimages.biz
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
Just a word of caution about using gases in cylinders. We used to use 99.999% nitrogen on our microscopes but discovered water and other contaminants in the gas lines. We asked the gas supplier and they checked a number of the "high purity" cylinders of different gases only to discover water, rust and other "crud" in them.
We now use the nitrogen gas blow off from a 3000 litre liquid nitrogen vessel with multiple fail-safes in the line and multiple oxygen level monitors (and safety lock-outs) in all the labs that utilise this source. We have a single cylinder as a back up if the main vessel has to be disconnected.
Cheers
Colin Veitch
Electron Microscopist CSIRO Materials Science and Engineering, Geelong Laboratory PO Box 21, BELMONT, Vic. 3216. Australia.
The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000.
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Wednesday, 27 April 2011 08:33 To: Veitch, Colin (CMSE, Geelong Belmont)
In any case, a single bottle is not nearly the hazard that plumbed in nitrogen is.
A. John Mardinly, ASU
-----Original Message----- X-from: Ken Converse [mailto:kenconverse-at-qualityimages.biz] Sent: Tuesday, April 26, 2011 3:19 PM To: John Mardinly; MSA Listserver
Ken; An "A" cylinder is 1.76 cubic feet internally, which at 3,000 psi is 200 atmospheres which should be more like 352 cubic feet of nitrogen gas.
Sent: Tuesday, April 26, 2011 11:49 AM To: John Mardinly
Each tank is typically 220 cubic feet and with a large room and open door, I wouldn't think that there is too big a risk. What I use in my shop is an oil-based compressor that gets filtered through a coalescing filter that I think is rated at .05 microns for oil mist. The filter has an indicator that goes from green to red if the efficiency drops too much. It's still green and has been in use for about 25 years and I've had no problems with contamination. I only use the Freon for leak detecting with a Varian Smart Gauge so I don't use much.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, April 26, 2011 2:13 PM To: kenconverse-at-qualityimages.biz
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
I just learned a few things in a compressed gas course I took recently, and that is that tanks should not ever be fully emptied because then they need to be purged and cleaned before being refilled so that they do not get contaminants in them. Trouble is 99% of gas users do not know this, and run tanks until nothing comes out. Then since they did not notify the gas vendor, they did not clean or purge the cylinder. Another thing is that the instructor said tanks are supposed to immersed in water to cool them while being refilled. Perhaps that is one way they can pick up water.
John Mardinly ASU
On Apr 26, 2011, at 4:09 PM, "Colin.Veitch-at-csiro.au" {Colin.Veitch-at-csiro.au} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } G'day, } } Just a word of caution about using gases in cylinders. We used to use 99.999% nitrogen on our microscopes but discovered water and other contaminants in the gas lines. We asked the gas supplier and they checked a number of the "high purity" cylinders of different gases only to discover water, rust and other "crud" in them. } } We now use the nitrogen gas blow off from a 3000 litre liquid nitrogen vessel with multiple fail-safes in the line and multiple oxygen level monitors (and safety lock-outs) in all the labs that utilise this source. We have a single cylinder as a back up if the main vessel has to be disconnected. } } Cheers } } Colin Veitch } } } Electron Microscopist } CSIRO Materials Science and Engineering, Geelong Laboratory } PO Box 21, BELMONT, Vic. 3216. Australia. } } colin.veitch-at-csiro.au } http://www.tft.csiro.au } } Tel: +61 (0) 3 5246 4000 } Mobile: 0438 538 475 } Fax: +61 (0) 3 5246 4811 } } The information contained in this e-mail message may be privileged or confidential information. If you are not an intended recipient, you may not copy, distribute or take any action in reliance on it. If you have received this message in error, please telephone CSIRO Materials Science and Engineering on +61 3 5246 4000. } } -----Original Message----- } X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] } Sent: Wednesday, 27 April 2011 08:33 } To: Veitch, Colin (CMSE, Geelong Belmont) } Subject: [Microscopy] problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } In any case, a single bottle is not nearly the hazard that plumbed in nitrogen is. } } A. John Mardinly, } ASU } } -----Original Message----- } X-from: Ken Converse [mailto:kenconverse-at-qualityimages.biz] } Sent: Tuesday, April 26, 2011 3:19 PM } To: John Mardinly; MSA Listserver } Subject: RE: [Microscopy] RE: problem with compressed air-thanks! } } Hi John, } In another life I often heard them referred to as 220 cubic foot cylinders. } I've never seen one with more than 2600psi, but that does come out to 305 } cubic feet which is a good deal more than 220. I stand corrected. } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] } Sent: Tuesday, April 26, 2011 4:53 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] RE: problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Ken; } An "A" cylinder is 1.76 cubic feet internally, which at 3,000 psi is } 200 atmospheres which should be more like 352 cubic feet of nitrogen gas. } } A. John Mardinly, } ASU } } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } } Sent: Tuesday, April 26, 2011 11:49 AM } To: John Mardinly } Subject: [Microscopy] problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Each tank is typically 220 cubic feet and with a large room and open door, I } wouldn't think that there is too big a risk. What I use in my shop is an } oil-based compressor that gets filtered through a coalescing filter that I } think is rated at .05 microns for oil mist. The filter has an indicator } that goes from green to red if the efficiency drops too much. It's still } green and has been in use for about 25 years and I've had no problems with } contamination. I only use the Freon for leak detecting with a Varian Smart } Gauge so I don't use much. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Sent: Tuesday, April 26, 2011 2:13 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] RE: problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I have always been cautious with my liquid N2 for the reasons John raises } below but have never thought about compressed N2 tanks. In my microtome } room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It } is a lot cheaper and environmentally nicer than using those old compressed } Freon cans of gas. Am I really running a serious risk here? It is a } relatively big room with an open door into the main lab. Comments? Tom } } } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } -----Original Message----- } X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] } Sent: Tuesday, April 26, 2011 12:05 PM } To: Phillips, Thomas E. } Subject: [Microscopy] problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I had an IDE air system on a 2010F fill up with water once when we went too } long without purging the compressor. The replacement system had an automatic } purge and an air dryer in series. Be advised that using nitrogen cylinders } for valves is ill advised: valve actuators tend to develop leaks over time, } and a catastrophic leak could not only deplete your cylinder rapidly, it } could fill the room with asphyxiant, which is an unexpected but lethal } consequence. There were multiple fatalities from nitrogen use when I was at } Intel. } } A. John Mardinly, } ASU } } } -----Original Message----- } X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] } Sent: Tuesday, April 26, 2011 1:20 AM } To: John Mardinly } Subject: [Microscopy] Re: problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks to all who offered their advices. I have unplugged all the lines and } they } all contained water, but I am quite sure that the water was lying in the } bottom } because the system was off so there was no air movement. We'll probably } replace } the lines because we are almost sure that the water contained some oil. It } is } still a mystery why this happened but I don't want to care about it anymore. } In } the future we'll connect an N2 bottle in place of compressed air for the } valves. } No way that I worry again to waste 600 000 euros worth of material for some } stupid moisture (you may replace "stupid" by other not-politically-correct } words } here, to better reflect my thoughts). } } Regards, } } Stephane } } } ==============================Original Headers============================== } 4, 36 -- From nizets2-at-yahoo.com Tue Apr 26 03:08:52 2011 } 4, 36 -- Received: from nm28-vm0.bullet.mail.sp2.yahoo.com } (nm28-vm0.bullet.mail.sp2.yahoo.com [98.139.91.234]) } 4, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p3Q88pYQ015973 } 4, 36 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 03:08:51 } -0500 } 4, 36 -- Received: from [98.139.91.65] by nm28.bullet.mail.sp2.yahoo.com } with NNFMP; 26 Apr 2011 08:08:51 -0000 } 4, 36 -- Received: from [98.139.91.18] by tm5.bullet.mail.sp2.yahoo.com with } NNFMP; 26 Apr 2011 08:08:51 -0000 } 4, 36 -- Received: from [127.0.0.1] by omp1018.mail.sp2.yahoo.com with } NNFMP; 26 Apr 2011 08:08:51 -0000 } 4, 36 -- X-Yahoo-Newman-Property: ymail-3 } 4, 36 -- X-Yahoo-Newman-Id: 344787.71901.bm-at-omp1018.mail.sp2.yahoo.com } 4, 36 -- Received: (qmail 14970 invoked by uid 60001); 26 Apr 2011 08:08:50 } -0000 } 4, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; } s=s1024; t=1303805330; bh=HSYhHsZakS09GHNRuXg2n0ZbE/NJot+vm5rgMBQm0U8=; } h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:I } n-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } b=Vz03dlxKuYysw2/5KPRZCWHgRLbgZaY2Qf8p1mcfnrykEeiDmgRN7/hYa2VnsrTN6JH0KXcsiR } FZ2NeqjNsNmyq01RRu9xMI8sPE3W5wTrwOAkleRRUBkhAXtHGB2E2ksgwVRf/9AoFu986EixPBmp } zziORKHCPLXWNiEMvm7a4= } 4, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; } 4, 36 -- s=s1024; d=yahoo.com; } 4, 36 -- } h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:I } n-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; } 4, 36 -- } b=PaJoBvxcCvFfqh1vPmteLI8rjZ1tLJYkAgAAJ+tRU7FGOznwl1zzovmwvI5F3jqLHtSQTBQ6Yv } GL+JvY3h1w2nB6TEuPfhz/jieBhE77/FTAhzgcfU54z8R0f0evbmASvHG34D7fzA0U14pLCr1k80 } DfwDK648MZumXsmCYKCME=; } 4, 36 -- Message-ID: {769007.12438.qm-at-web110810.mail.gq1.yahoo.com} } 4, 36 -- X-YMail-OSG: HepaM6AVM1khQK2.Rmk5GIgON7Mrn4ZbhMMPIQi_WK6qElB } 4, 36 -- ouq1vWcGO.vFXBI85tkbnmf7YiFkFBC_Ko6KPqAAFjmAOBxTJe_1s75nNxpP } 4, 36 -- VN7bnUUnrvp0fuCb4HPvZ97ECULJuDMTn6IB6fkeMxv6AwRXK3H8QM_nNLQQ } 4, 36 -- RUFR0npYlGMcHJkNlD9pt2SAh70Ay4aW8A0vPfkxdKF1KO5idhHR892R7ncM } 4, 36 -- U1b93ZB5zNnk2IvjzSMRWkaodm8L9yZNonyF2EpZgCiOqY374jGRpLFyVqUG } 4, 36 -- i5hMEcuc2OBhJqcqE88_crf5i6ukFmYVhzmvZfSERUqllLtcrpttycSbsDpf } 4, 36 -- dr7fvrRCyi8uHaiOhNV26D89A96J49dS7GR_luZ_ougvrEyWvfbthricIC_X } 4, 36 -- J619ZWME8RhAp6A-- } 4, 36 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via } HTTP; Tue, 26 Apr 2011 01:08:50 PDT } 4, 36 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.110.299900 } 4, 36 -- References: {201104220714.p3M7EEmR018140-at-ns.microscopy.com} } {20110426093538.524cecc6-at-emf01} } 4, 36 -- Date: Tue, 26 Apr 2011 01:08:50 -0700 (PDT) } 4, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 4, 36 -- Subject: Re: [Microscopy] problem with compressed air-thanks! } 4, 36 -- To: microscopy-at-microscopy.com } 4, 36 -- In-Reply-To: {20110426093538.524cecc6-at-emf01} } 4, 36 -- MIME-Version: 1.0 } 4, 36 -- Content-Type: text/plain; charset=iso-8859-1 } 4, 36 -- Content-Transfer-Encoding: 8bit } 4, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3Q88pYQ015973 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 13, 39 -- From John.Mardinly-at-asu.edu Tue Apr 26 12:04:32 2011 } 13, 39 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) } 13, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3QH4WAl000839 } 13, 39 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 12:04:32 } -0500 } 13, 39 -- X-ASG-Debug-ID: 1303837470-7dc770380002-4CH8be } 13, 39 -- Received: from exhub2.asurite.ad.asu.edu } (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet7.asu.edu with ESMTP id } vysrgnAIHMvENT4L (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO) for } {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 10:04:30 -0700 (MST) } 13, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 13, 39 -- X-ASG-Whitelist: Client } 13, 39 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by } 13, 39 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP } Server id } 13, 39 -- 8.1.436.0; Tue, 26 Apr 2011 10:04:30 -0700 } 13, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by } 13, 39 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); } Tue, 26 Apr } 13, 39 -- 2011 10:04:30 -0700 } 13, 39 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 13, 39 -- Content-Class: urn:content-classes:message } 13, 39 -- MIME-Version: 1.0 } 13, 39 -- Content-Type: text/plain; charset="iso-8859-1" } 13, 39 -- Subject: RE: [Microscopy] Re: problem with compressed air-thanks! } 13, 39 -- Date: Tue, 26 Apr 2011 10:04:30 -0700 } 13, 39 -- X-ASG-Orig-Subj: RE: [Microscopy] Re: problem with compressed } air-thanks! } 13, 39 -- Message-ID: } {95FFD84122D92D4F913DE75095E9C42E0661035E-at-EX05.asurite.ad.asu.edu} } 13, 39 -- In-Reply-To: {201104260820.p3Q8KMJn031910-at-ns.microscopy.com} } 13, 39 -- X-MS-Has-Attach: } 13, 39 -- X-MS-TNEF-Correlator: } 13, 39 -- Thread-Topic: [Microscopy] Re: problem with compressed air-thanks! } 13, 39 -- thread-index: AcwD60gmWQefcwp0SuCAtGaFJ+xD/gASAcSg } 13, 39 -- References: {201104260820.p3Q8KMJn031910-at-ns.microscopy.com} } 13, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 13, 39 -- To: {nizets2-at-yahoo.com} } 13, 39 -- CC: {microscopy-at-microscopy.com} } 13, 39 -- X-OriginalArrivalTime: 26 Apr 2011 17:04:30.0560 (UTC) } FILETIME=[02051600:01CC0434] } 13, 39 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] } 13, 39 -- X-Barracuda-Start-Time: 1303837470 } 13, 39 -- X-Barracuda-Encrypted: RC4-MD5 } 13, 39 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi } 13, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu } 13, 39 -- Content-Transfer-Encoding: 8bit } 13, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3QH4WAl000839 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 26, 30 -- From PhillipsT-at-missouri.edu Tue Apr 26 13:10:12 2011 } 26, 30 -- Received: from mxnip01-missouri-out.um.umsystem.edu } (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) } 26, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3QIABFH013193 } 26, 30 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 13:10:12 } -0500 } 26, 30 -- X-IronPort-Anti-Spam-Filtered: true } 26, 30 -- X-IronPort-Anti-Spam-Result: } AkwBABQKt03Raua2/2dsb2JhbACXdg6NHkF3uFYBhQ6IbwKDF4JdBIV9kDuFeE8 } 26, 30 -- Received: from um-nhub02.um.umsystem.edu ([209.106.230.182]) } 26, 30 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 26 Apr 2011 } 13:10:03 -0500 } 26, 30 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by } 26, 30 -- UM-NHUB02.um.umsystem.edu ([209.106.230.182]) with mapi; Tue, 26 } Apr 2011 } 26, 30 -- 13:10:02 -0500 } 26, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 26, 30 -- To: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} , } 26, 30 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 26, 30 -- Date: Tue, 26 Apr 2011 13:10:02 -0500 } 26, 30 -- Subject: RE: [Microscopy] problem with compressed air-thanks! } 26, 30 -- Thread-Topic: [Microscopy] problem with compressed air-thanks! } 26, 30 -- Thread-Index: AcwENBounNL0ns62Td+L69/SjFBkPAACKvBw } 26, 30 -- Message-ID: } {616B99DC91A1D64A9BC81BA4326586DF267BC0F45C-at-UM-EMAIL05.um.umsystem.edu} } 26, 30 -- References: {201104261705.p3QH503e002034-at-ns.microscopy.com} } 26, 30 -- In-Reply-To: {201104261705.p3QH503e002034-at-ns.microscopy.com} } 26, 30 -- Accept-Language: en-US } 26, 30 -- Content-Language: en-US } 26, 30 -- X-MS-Has-Attach: } 26, 30 -- X-MS-TNEF-Correlator: } 26, 30 -- acceptlanguage: en-US } 26, 30 -- Content-Type: text/plain; charset="iso-8859-1" } 26, 30 -- MIME-Version: 1.0 } 26, 30 -- Content-Transfer-Encoding: 8bit } 26, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3QIABFH013193 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 37, 28 -- From kenconverse-at-qualityimages.biz Tue Apr 26 13:41:58 2011 } 37, 28 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.123]) } 37, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3QIfwOi029875 } 37, 28 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 13:41:58 } -0500 } 37, 28 -- X-Authority-Analysis: v=1.1 } cv=u0GDbnsGpYF3ufkDf8oJFk+kgusfHOwmXSY+Z6GJvsg= c=1 sm=0 a=T8SKUq4ufZkA:10 } a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 } a=KKXDUkkEAAAA:8 a=CjxXgO3LAAAA:8 a=vCpxnH_lAAAA:8 a=3eTFaH3e67iHxxlzM4gA:9 } a=QHQr4SiJOKyYSeaP9-gA:7 a=wPNLvfGTeEIA:10 a=hoF1kz9iuq0A:10 } a=5JUlSvKOtR0A:10 a=6XF2fXZ5PSMA:10 a=TiKuPFr2l_4A:10 a=RyjLG6jaqdoA:10 } a=RrvOwbEyzwoA:10 a=3_2eAsHTqJoA:10 a=rC2wZJ5BpNYA:10 a=G91thX30KR8A:10 } a=qiWi7Edwir4A:10 a=NWVoK91CQyQA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 37, 28 -- X-Cloudmark-Score: 0 } 37, 28 -- X-Originating-IP: 72.224.149.57 } 37, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1768] helo=Ken) } 37, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from } {kenconverse-at-qualityimages.biz} ) } 37, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP } 37, 28 -- id 72/FE-27107-5F117BD4; Tue, 26 Apr 2011 18:41:58 +0000 } 37, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 37, 28 -- To: {PhillipsT-at-missouri.edu} , "MSA Listserver" } {microscopy-at-microscopy.com} } 37, 28 -- Subject: RE: [Microscopy] RE: problem with compressed air-thanks! } 37, 28 -- Date: Tue, 26 Apr 2011 14:41:51 -0400 } 37, 28 -- Message-ID: {A4BD978866294831ACAAD1325F663620-at-Ken} } 37, 28 -- MIME-Version: 1.0 } 37, 28 -- Content-Type: text/plain; } 37, 28 -- charset="iso-8859-1" } 37, 28 -- X-Priority: 3 (Normal) } 37, 28 -- X-MSMail-Priority: Normal } 37, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 37, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5994 } 37, 28 -- In-Reply-To: {201104261812.p3QICgor016700-at-ns.microscopy.com} } 37, 28 -- Thread-Index: AcwEPYlNx8O26fSfSUW9GrQfyzeLZQAAp5kg } 37, 28 -- Importance: Normal } 37, 28 -- Content-Transfer-Encoding: 8bit } 37, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3QIfwOi029875 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 47, 40 -- From John.Mardinly-at-asu.edu Tue Apr 26 15:50:35 2011 } 47, 40 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) } 47, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3QKoZ3F016025 } 47, 40 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 15:50:35 } -0500 } 47, 40 -- X-ASG-Debug-ID: 1303851034-51f4038e0001-4CH8be } 47, 40 -- Received: from exhub2.asurite.ad.asu.edu } (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet7.asu.edu with ESMTP id } GD8PCK2GN52SRCdU (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Tue, 26 } Apr 2011 13:50:34 -0700 (MST) } 47, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 47, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 } 47, 40 -- X-ASG-Whitelist: Client } 47, 40 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by } 47, 40 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP } Server id } 47, 40 -- 8.1.436.0; Tue, 26 Apr 2011 13:50:33 -0700 } 47, 40 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by } 47, 40 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); } Tue, 26 Apr } 47, 40 -- 2011 13:50:28 -0700 } 47, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 47, 40 -- Content-Class: urn:content-classes:message } 47, 40 -- MIME-Version: 1.0 } 47, 40 -- Content-Type: text/plain; charset="iso-8859-1" } 47, 40 -- Subject: RE: [Microscopy] problem with compressed air-thanks! } 47, 40 -- Date: Tue, 26 Apr 2011 13:50:28 -0700 } 47, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] problem with compressed } air-thanks! } 47, 40 -- Message-ID: } {95FFD84122D92D4F913DE75095E9C42E06610369-at-EX05.asurite.ad.asu.edu} } 47, 40 -- In-Reply-To: {201104261849.p3QInOCa013272-at-ns.microscopy.com} } 47, 40 -- X-MS-Has-Attach: } 47, 40 -- X-MS-TNEF-Correlator: } 47, 40 -- Thread-Topic: [Microscopy] problem with compressed air-thanks! } 47, 40 -- thread-index: AcwEQqsL8uTSumP6SXqERtxHPtQiwQAEDI0Q } 47, 40 -- References: {201104261849.p3QInOCa013272-at-ns.microscopy.com} } 47, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 47, 40 -- To: {kenconverse-at-qualityimages.biz} } 47, 40 -- CC: {microscopy-at-microscopy.com} } 47, 40 -- X-OriginalArrivalTime: 26 Apr 2011 20:50:28.0616 (UTC) } FILETIME=[93404080:01CC0453] } 47, 40 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] } 47, 40 -- X-Barracuda-Start-Time: 1303851034 } 47, 40 -- X-Barracuda-Encrypted: RC4-MD5 } 47, 40 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi } 47, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu } 47, 40 -- Content-Transfer-Encoding: 8bit } 47, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3QKoZ3F016025 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 61, 40 -- From John.Mardinly-at-asu.edu Tue Apr 26 17:32:44 2011 } 61, 40 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) } 61, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QMWhsW017969 } 61, 40 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 17:32:43 -0500 } 61, 40 -- X-ASG-Debug-ID: 1303857162-158a55d50001-4CH8be } 61, 40 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet6.asu.edu with ESMTP id ysEwDJITMj1L8GRC (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Tue, 26 Apr 2011 15:32:42 -0700 (MST) } 61, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 61, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 } 61, 40 -- X-ASG-Whitelist: Client } 61, 40 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by } 61, 40 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id } 61, 40 -- 8.1.436.0; Tue, 26 Apr 2011 15:32:42 -0700 } 61, 40 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by } 61, 40 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Tue, 26 Apr } 61, 40 -- 2011 15:32:42 -0700 } 61, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 } 61, 40 -- Content-Class: urn:content-classes:message } 61, 40 -- MIME-Version: 1.0 } 61, 40 -- Content-Type: text/plain; charset="iso-8859-1" } 61, 40 -- Subject: RE: [Microscopy] RE: problem with compressed air-thanks! } 61, 40 -- Date: Tue, 26 Apr 2011 15:32:41 -0700 } 61, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: problem with compressed air-thanks! } 61, 40 -- Message-ID: {95FFD84122D92D4F913DE75095E9C42E0661036B-at-EX05.asurite.ad.asu.edu} } 61, 40 -- In-Reply-To: {1B8554DC2553473EB4D0D7CDF78D35B1-at-Ken} } 61, 40 -- X-MS-Has-Attach: } 61, 40 -- X-MS-TNEF-Correlator: } 61, 40 -- Thread-Topic: [Microscopy] RE: problem with compressed air-thanks! } 61, 40 -- thread-index: AcwEU/JAHDJHfP5NTLe74XHQja/dLAAC4qSAAACNAuA= } 61, 40 -- References: {201104262053.p3QKr7Rq019501-at-ns.microscopy.com} {1B8554DC2553473EB4D0D7CDF78D35B1-at-Ken} } 61, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 61, 40 -- To: Ken Converse {kenconverse-at-qualityimages.biz} } 61, 40 -- CC: {microscopy-at-microscopy.com} } 61, 40 -- X-OriginalArrivalTime: 26 Apr 2011 22:32:42.0246 (UTC) FILETIME=[DB2DEA60:01CC0461] } 61, 40 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] } 61, 40 -- X-Barracuda-Start-Time: 1303857162 } 61, 40 -- X-Barracuda-Encrypted: RC4-MD5 } 61, 40 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi } 61, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu } 61, 40 -- Content-Transfer-Encoding: 8bit } 61, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3QMWhsW017969 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 85, 38 -- From prvs=090db5830=Colin.Veitch-at-csiro.au Tue Apr 26 17:58:35 2011 } 85, 38 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) } 85, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3QMwX8o001923 } 85, 38 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2011 17:58:34 -0500 } 85, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 85, 38 -- d=csiro.au; i=Colin.Veitch-at-csiro.au; q=dns/txt; } 85, 38 -- s=email; t=1303858714; x=1335394714; } 85, 38 -- h=from:to:date:subject:message-id:references:in-reply-to: } 85, 38 -- content-transfer-encoding:mime-version; } 85, 38 -- bh=mENuuunUZ92TBMM+5xrRRrRFo+Gdsf1d9f0g9d/bJV0=; } 85, 38 -- b=rFSJXu+chhJX2TPQT4vVmSJcw8kqp186z/lm2n2SQLuFRcW8jh1oK/i0 } 85, 38 -- WSy4nYtH13Y0apnvSBnxDvT+616n739eWWYYYHZYRDmKPhpo3np+tdVe1 } 85, 38 -- 40uJPVuBQLvwx1f; } 85, 38 -- X-IronPort-AV: E=Sophos;i="4.64,271,1301839200"; } 85, 38 -- d="scan'208";a="19568313" } 85, 38 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127]) } 85, 38 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 27 Apr 2011 08:58:28 +1000 } 85, 38 -- Received: from EXVIC-MBX02.nexus.csiro.au ([138.194.81.122]) by } 85, 38 -- exvic-htca02.nexus.csiro.au ([138.194.81.127]) with mapi; Wed, 27 Apr 2011 } 85, 38 -- 08:58:28 +1000 } 85, 38 -- From: {Colin.Veitch-at-csiro.au} } 85, 38 -- To: {microscopy-at-microscopy.com} } 85, 38 -- Date: Wed, 27 Apr 2011 08:58:27 +1000 } 85, 38 -- Subject: [Microscopy] problem with compressed air-thanks! } 85, 38 -- Thread-Topic: [Microscopy] problem with compressed air-thanks! } 85, 38 -- Thread-Index: AcwEYfEaRBeP/PCVSt6fOldTvYQPzgAAnV9w } 85, 38 -- Message-ID: {4AA5DD81F68A934F8C80DA2BF125163D01A98BE39E22-at-EXVIC-MBX02.nexus.csiro.au} } 85, 38 -- References: {201104262232.p3QMWng0018062-at-ns.microscopy.com} } 85, 38 -- In-Reply-To: {201104262232.p3QMWng0018062-at-ns.microscopy.com} } 85, 38 -- Accept-Language: en-US, en-AU } 85, 38 -- Content-Language: en-US } 85, 38 -- X-MS-Has-Attach: } 85, 38 -- X-MS-TNEF-Correlator: } 85, 38 -- acceptlanguage: en-US, en-AU } 85, 38 -- Content-Type: text/plain; charset="iso-8859-1" } 85, 38 -- MIME-Version: 1.0 } 85, 38 -- Content-Transfer-Encoding: 8bit } 85, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3QMwX8o001923 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 39 -- From John.Mardinly-at-asu.edu Tue Apr 26 19:06:06 2011 5, 39 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3R065Jr019471 5, 39 -- for {Microscopy-at-Microscopy.com} ; Tue, 26 Apr 2011 19:06:05 -0500 5, 39 -- X-ASG-Debug-ID: 1303862765-027c23510001-FOsErg 5, 39 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet6.asu.edu with ESMTP id zeJYKiTfqD1K5j06 (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Tue, 26 Apr 2011 17:06:05 -0700 (MST) 5, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 5, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 5, 39 -- X-ASG-Whitelist: Client 5, 39 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.207) by 5, 39 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 5, 39 -- 8.1.436.0; Tue, 26 Apr 2011 17:06:04 -0700 5, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 5, 39 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Tue, 26 Apr 5, 39 -- 2011 17:06:04 -0700 5, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 5, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Wed, 27 Apr 2011 5, 39 -- 00:06:04 +0000 5, 39 -- Subject: Re: [Microscopy] problem with compressed air-thanks! 5, 39 -- References: {201104262308.p3QN8wmv017778-at-ns.microscopy.com} 5, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] problem with compressed air-thanks! 5, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} 5, 39 -- Content-Type: text/plain; charset="us-ascii" 5, 39 -- thread-topic: [Microscopy] problem with compressed air-thanks! 5, 39 -- thread-index: AcwEbuZKH9z3/cFzSK6OKD/N1iKwaw== 5, 39 -- In-Reply-To: {201104262308.p3QN8wmv017778-at-ns.microscopy.com} 5, 39 -- Message-ID: {4AEC76A7-43AB-4D2D-831E-975C0B51EAB4-at-exchange.asu.edu} 5, 39 -- Date: Tue, 26 Apr 2011 17:06:26 -0700 5, 39 -- To: {Colin.Veitch-at-csiro.au} 5, 39 -- CC: "MSA Listserver" {Microscopy-at-Microscopy.com} 5, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) 5, 39 -- X-OriginalArrivalTime: 27 Apr 2011 00:06:04.0855 (UTC) FILETIME=[E6983470:01CC046E] 5, 39 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 5, 39 -- X-Barracuda-Start-Time: 1303862765 5, 39 -- X-Barracuda-Encrypted: RC4-MD5 5, 39 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 5, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu 5, 39 -- Content-Transfer-Encoding: 8bit 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3R065Jr019471 ==============================End of - Headers==============================
John makes an good point about plumbed in N2 (or should we be saying "coppered in" nowadays?).
I think this is a real hazard, which is why all our N2 lines terminate in a separately ventilated equipment gallery that surrounds our lab complex, not to mention that we have O2 monitors as well. All the fume hoods and room ventilation are also on emergency power in addition to the instruments.
Another point worth raising is that each instrument has different requirements for psi delivery so at each instrument station in the equipment gallery we provide a two stage regulator to take it from 110 psi to a more moderate pressure, say 10 to 60 psi and then for those instruments that require further stepping down, an additional precision low pressure regulator for regulating from zero to 2 or 3 psi. john
At 03:36 PM 4/26/2011, you wrote:
} In any case, a single bottle is not nearly the hazard that plumbed } in nitrogen is. } } A. John Mardinly, } ASU } } -----Original Message----- } X-from: Ken Converse [mailto:kenconverse-at-qualityimages.biz] } Sent: Tuesday, April 26, 2011 3:19 PM } To: John Mardinly; MSA Listserver } Subject: RE: [Microscopy] RE: problem with compressed air-thanks! } } Hi John, } In another life I often heard them referred to as 220 cubic foot cylinders. } I've never seen one with more than 2600psi, but that does come out to 305 } cubic feet which is a good deal more than 220. I stand corrected. } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] } Sent: Tuesday, April 26, 2011 4:53 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] RE: problem with compressed air-thanks! } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
For general advice on preserving lipids for TEM, the expert is Lacey Samuels at UBC, Vancouver. She's also done quite a bit of immuno-EM.
Or your colleague could look up:
Mamun EA, Cantrill LC, Overall RL, Sutton BG 2005 Cellular organisation in meiotic and early post-meiotic rice anthers. Cell Biology International 29: 903-913
or here's an unpublished protocol from a thesis - really spectacular images of pollen at various stages of development in rice anthers:
The cryofixation protocol for immunolabelling was modified from Paxson-Sowders et al. (2001). Anthers at the required stages were cut and placed in specimen holders pre-coated with 7.5% (w/v) lecithin in chloroform and filled with 0.2M sucrose. Then samples were frozen in a Leica EM PACT Freezer (Leica, Germany). Following freezing, samples were freeze-substituted in 3% paraformaldehyde and 0.5% uranyl acetate in anhydrous acetone at -90ºC for 60 hours, -60ºC for 12 hours, -30ºC for 6 hours and then brought up to 20ºC with a temperature increase rate of +5ºC per hour in a Leica EM AFS (automatic freeze substitution) machine (Leica, Germany). Following this the anthers were rinsed 3 times in anhydrous acetone and infiltrated in a graded acetone/LR gold acrylic resin mixture and 100% LR gold resin for 5 days with two changes of fresh resin per day and polymerised under UV at -20ºC in the Leica EM AFS for 24 hours.
Immunolabelling techniques were modified from methods for indirect immunogold labelling of ultra-thin sections for TEM (Cole, 1996) and immunofluorescence labelling (Harper et al., 1996). One micrometer sections of LR-Gold embedded blocks were placed on poly-L-lysine coated slides and were blocked with 200 μl per sample of blocking buffer containing phosphate buffered saline (PBS) (0.8% NaCl, 0.02% KCl, 0.115% Na2HPO4, KH2PO4 and 0.02% NaN3, pH6.9) and 1% BSA at room temperature for 1 hour. Then the sections were incubated in a 1:100 solution of primary antibodies for callose and callose synthase in blocking buffer at 4ºC overnight at a rate of 20μl per sample. Sections were washed 3 times with PBS for 5 minutes each, followed by incubation of secondary antibody (1:50 dilution with the same blocking buffer) at 37 ºC for 3 hours. Sections were washed again 3 times with PBS for 5 minutes each, followed by background staining with propidium iodide (10μg/ml) in PBS for 10 minutes. Sections were washed twice with deionized water for 5 minutes each and then mounted in anti-fade solution, Citifluor (Citifluor, London, UK). Slides were covered and sealed with nail polish, and then allowed to air-dry for 10 minutes. Immunogold-labelling techniques at the electron microscopy level were modified based on indirect immunogold labelling of ultra-thin sections for TEM (Cole, 1996). Ultra-thin sections from LR gold blocks were placed on pioloform film coated nickel grids (100 hexagonal mesh) and were blocked in 30 μl/grid of blocking buffer containing phosphate buffered saline plus Tween 20 (PBST) (0.8 % NaCl, 0.02% KCl, 0.115% Na2HPO4, KH2PO4, 0.02% NaN3 and 0.5% Tween 20, pH6.9) and 1% BSA at room temperature for 1 hour. Then the sections were incubated in 20μl/grid of 1:100 primary antibodies for callose and callose synthase and 1:400 for β-1,3-glucanase in the blocking buffer at room temperature for 2 hours. Sections were washed with PBST 3 times for 5 minutes each, followed by incubation with secondary antibody (1:30 dilution with the blocking buffer) at room temperature for 1 hour. Sections were washed again 3 times with PBST and then with distilled water twice all for 5 minutes each, followed by background staining with saturated uranyl acetate (in 50% ethanol) and Reynold’s lead citrate for 10 minutes each. The sections were rinsed twice with distilled water for 1 minute after staining.
cheers, Rosemary White
On 19/04/11 4:31 AM, "Ann-Fook.Yang-at-AGR.GC.CA" {Ann-Fook.Yang-at-AGR.GC.CA} wrote:
Good Morning We have two 160 liter LN DeWars for general usage in our lab. We tap off the head pressure with standard compressed air connections typical of what is used in auto repair shops connected to a 3/8” OD Dekoron tubing manifold to all our labs. The head pressure is normally about 20 PSI. We use this free, dry and very pure nitrogen gas to vent, purge and backfill all our instruments with a low pressure regulator at the instrument. It has worked well without any problems for quite some time. With two supply DeWars we can switch from one source to the other when either supply is empty resulting in a constant nitrogen source. 20PSI may not be enough for some applications but for most of our uses it is adequate. Fran Laabs ________________________________________ X-from: kenconverse-at-qualityimages.biz [kenconverse-at-qualityimages.biz] Sent: Tuesday, April 26, 2011 1:42 PM To: fclaabs-at-iastate.edu
Each tank is typically 220 cubic feet and with a large room and open door, I wouldn't think that there is too big a risk. What I use in my shop is an oil-based compressor that gets filtered through a coalescing filter that I think is rated at .05 microns for oil mist. The filter has an indicator that goes from green to red if the efficiency drops too much. It's still green and has been in use for about 25 years and I've had no problems with contamination. I only use the Freon for leak detecting with a Varian Smart Gauge so I don't use much.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Tuesday, April 26, 2011 2:13 PM To: kenconverse-at-qualityimages.biz
I have always been cautious with my liquid N2 for the reasons John raises below but have never thought about compressed N2 tanks. In my microtome room, I run 3 N2 tanks to blow off debris from my ultramicrotome knives. It is a lot cheaper and environmentally nicer than using those old compressed Freon cans of gas. Am I really running a serious risk here? It is a relatively big room with an open door into the main lab. Comments? Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu] Sent: Tuesday, April 26, 2011 12:05 PM To: Phillips, Thomas E.
I had an IDE air system on a 2010F fill up with water once when we went too long without purging the compressor. The replacement system had an automatic purge and an air dryer in series. Be advised that using nitrogen cylinders for valves is ill advised: valve actuators tend to develop leaks over time, and a catastrophic leak could not only deplete your cylinder rapidly, it could fill the room with asphyxiant, which is an unexpected but lethal consequence. There were multiple fatalities from nitrogen use when I was at Intel.
A. John Mardinly, ASU
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Tuesday, April 26, 2011 1:20 AM To: John Mardinly
Thanks to all who offered their advices. I have unplugged all the lines and they all contained water, but I am quite sure that the water was lying in the bottom because the system was off so there was no air movement. We'll probably replace the lines because we are almost sure that the water contained some oil. It is still a mystery why this happened but I don't want to care about it anymore. In the future we'll connect an N2 bottle in place of compressed air for the valves. No way that I worry again to waste 600 000 euros worth of material for some stupid moisture (you may replace "stupid" by other not-politically-correct words here, to better reflect my thoughts).
I was wondering what others may be using to make their labels for glass slides permanent and insoluble to ultrasonic cleaning in ETOH. I’ve seen some slides where someone had labeled with a Sharpie, then applied something akin to clear nail polish (… except that I’d like to use something similar for covering a tape label). Whatever your method, would it be too much to ask that it also hold up to acetone?
TIA :o) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland SEM-MLA Calendar: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
==============================Original Headers============================== 6, 27 -- From michael-at-shaffer.net Wed Apr 27 11:16:56 2011 6, 27 -- Received: from smtprelay.hostedemail.com (smtprelay0004.hostedemail.com [216.40.44.4]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RGGu4K004116 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 11:16:56 -0500 6, 27 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 6, 27 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id 519BABC5A78 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 16:16:56 +0000 (UTC) 6, 27 -- X-Panda: scanned! 6, 27 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574 6, 27 -- X-Filterd-Recvd-Size: 1414 6, 27 -- Received: from shaffer (mshaffer.creait.mun.ca [134.153.131.207]) 6, 27 -- (Authenticated sender: michael-at-shaffer.net) 6, 27 -- by omf02.hostedemail.com (Postfix) with ESMTP 6, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 16:16:55 +0000 (UTC) 6, 27 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 27 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 27 -- Subject: making slide labels permanent 6, 27 -- Date: Wed, 27 Apr 2011 13:46:54 -0230 6, 27 -- Message-ID: {004501cc04f6$86d78940$94869bc0$-at-net} 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="iso-8859-1" 6, 27 -- X-Mailer: Microsoft Office Outlook 12.0 6, 27 -- Thread-Index: AcwE51huxqC4s4xVSF2Kz//2As/RWw== 6, 27 -- Content-Language: en-ca 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3RGGu4K004116 ==============================End of - Headers==============================
What works surprisingly well for us is the Brother P-Touch label makers.. the medium sized ones with a built in keyboard are great (don't need a computer) and can fit various height labels (up to 1"). Easily available from any office supply place (although you might have to order the larger sizes).
The labels are very robust and stand up quite well to a lot of abuse in our lab - heat, chemicals etc.
Chris Pawlowicz, B.Eng Manager, Lab Development UBM TechInsights 3000 Solandt Rd Ottawa ON Canada K2K 2X2 T: +1 613 576 0150 F: +1 613 599 6501 E: cpawlowicz-at-ubmtechinsights.com
I was wondering what others may be using to make their labels for glass slides permanent and insoluble to ultrasonic cleaning in ETOH. I've seen some slides where someone had labeled with a Sharpie, then applied something akin to clear nail polish (... except that I'd like to use something similar for covering a tape label). Whatever your method, would it be too much to ask that it also hold up to acetone?
TIA :o) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland SEM-MLA Calendar: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
CONFIDENTIALITY NOTICE: This e-mail transmission, and any documents, files or previous e-mail messages attached to it may contain confidential information that is legally privileged. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this transmission is STRICTLY PROHIBITED. If you have received this transmission in error, please immediately notify the sender. Please destroy the original transmission and its attachments without reading or saving in any manner. Thank you in advance for your cooperation.
If you have access to a hospital histology lab, ask the histotechs what they use (or borrow one to test).
My histotech uses a STATMARK PEN from StatLab Medical Products in Lewisville KY (800-442-3573) http://www.statlab.com. They are moderately expensive and need to be capped to ensure they don't dry out, but they work.
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
-----Original Message----- X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net] Sent: Wednesday, April 27, 2011 9:17 AM To: Cromey, Douglas W - (dcromey)
I was wondering what others may be using to make their labels for glass slides permanent and insoluble to ultrasonic cleaning in ETOH. I've seen some slides where someone had labeled with a Sharpie, then applied something akin to clear nail polish (. except that I'd like to use something similar for covering a tape label). Whatever your method, would it be too much to ask that it also hold up to acetone?
TIA :o) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland SEM-MLA Calendar: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
==============================Original Headers============================== 6, 27 -- From michael-at-shaffer.net Wed Apr 27 11:16:56 2011 6, 27 -- Received: from smtprelay.hostedemail.com (smtprelay0004.hostedemail.com [216.40.44.4]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RGGu4K004116 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 11:16:56 -0500 6, 27 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 6, 27 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id 519BABC5A78 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 16:16:56 +0000 (UTC) 6, 27 -- X-Panda: scanned! 6, 27 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574 6, 27 -- X-Filterd-Recvd-Size: 1414 6, 27 -- Received: from shaffer (mshaffer.creait.mun.ca [134.153.131.207]) 6, 27 -- (Authenticated sender: michael-at-shaffer.net) 6, 27 -- by omf02.hostedemail.com (Postfix) with ESMTP 6, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 16:16:55 +0000 (UTC) 6, 27 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 27 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 27 -- Subject: making slide labels permanent 6, 27 -- Date: Wed, 27 Apr 2011 13:46:54 -0230 6, 27 -- Message-ID: {004501cc04f6$86d78940$94869bc0$-at-net} 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="iso-8859-1" 6, 27 -- X-Mailer: Microsoft Office Outlook 12.0 6, 27 -- Thread-Index: AcwE51huxqC4s4xVSF2Kz//2As/RWw== 6, 27 -- Content-Language: en-ca 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3RGGu4K004116 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 47 -- From dcromey-at-email.arizona.edu Wed Apr 27 11:45:29 2011 22, 47 -- Received: from VA3EHSOBE006.bigfish.com (va3ehsobe006.messaging.microsoft.com [216.32.180.16]) 22, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RGjT30001945 22, 47 -- for {Microscopy-at-Microscopy.Com} ; Wed, 27 Apr 2011 11:45:29 -0500 22, 47 -- Received: from mail159-va3-R.bigfish.com (10.7.14.238) by 22, 47 -- VA3EHSOBE006.bigfish.com (10.7.40.26) with Microsoft SMTP Server id 22, 47 -- 14.1.225.8; Wed, 27 Apr 2011 16:45:28 +0000 22, 47 -- Received: from mail159-va3 (localhost.localdomain [127.0.0.1]) by 22, 47 -- mail159-va3-R.bigfish.com (Postfix) with ESMTP id D6B447404DA; Wed, 27 Apr 22, 47 -- 2011 16:45:28 +0000 (UTC) 22, 47 -- X-SpamScore: -24 22, 47 -- X-BigFish: VS-24(zz9371O62a3K7f0R542Mzz1202hzz8275bh8275chz32i87h2a8h668h839h61h) 22, 47 -- X-Spam-TCS-SCL: 0:0 22, 47 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:VA3DIAHUB040.RED001.local;RD:smtp801.microsoftonline.com;EFVD:NLI 22, 47 -- X-FB-DOMAIN-IP-MATCH: fail 22, 47 -- Received: from mail159-va3 (localhost.localdomain [127.0.0.1]) by mail159-va3 22, 47 -- (MessageSwitch) id 1303922728603619_9447; Wed, 27 Apr 2011 16:45:28 +0000 22, 47 -- (UTC) 22, 47 -- Received: from VA3EHSMHS025.bigfish.com (unknown [10.7.14.253]) by 22, 47 -- mail159-va3.bigfish.com (Postfix) with ESMTP id 9072D14F0001; Wed, 27 Apr 22, 47 -- 2011 16:45:28 +0000 (UTC) 22, 47 -- Received: from VA3DIAHUB040.RED001.local (65.55.171.153) by 22, 47 -- VA3EHSMHS025.bigfish.com (10.7.99.35) with Microsoft SMTP Server (TLS) id 22, 47 -- 14.1.225.8; Wed, 27 Apr 2011 16:45:26 +0000 22, 47 -- Received: from VA3DIAXVS211.RED001.local ([10.32.56.197]) by 22, 47 -- VA3DIAHUB040.RED001.local ([10.32.21.114]) with mapi; Wed, 27 Apr 2011 22, 47 -- 09:45:26 -0700 22, 47 -- From: "Cromey, Douglas W - (dcromey)" {dcromey-at-email.arizona.edu} 22, 47 -- To: "michael-at-shaffer.net" {michael-at-shaffer.net} 22, 47 -- CC: "Microscopy listserv (Microscopy-at-Microscopy.Com)" 22, 47 -- {Microscopy-at-Microscopy.Com} 22, 47 -- Date: Wed, 27 Apr 2011 09:45:14 -0700 22, 47 -- Subject: RE: [Microscopy] making slide labels permanent 22, 47 -- Thread-Topic: [Microscopy] making slide labels permanent 22, 47 -- Thread-Index: AcwE9p7ndGntwvQVQBa0+TbbTfKN1wAAy9/g 22, 47 -- Message-ID: {33EA1C13F421004C924AD94E7BF09A7D049AC13809-at-VA3DIAXVS211.RED001.local} 22, 47 -- References: {201104271617.p3RGHMHe004858-at-ns.microscopy.com} 22, 47 -- In-Reply-To: {201104271617.p3RGHMHe004858-at-ns.microscopy.com} 22, 47 -- Accept-Language: en-US 22, 47 -- Content-Language: en-US 22, 47 -- X-MS-Has-Attach: 22, 47 -- X-MS-TNEF-Correlator: 22, 47 -- acceptlanguage: en-US 22, 47 -- Content-Type: text/plain; charset="iso-8859-1" 22, 47 -- MIME-Version: 1.0 22, 47 -- Content-Transfer-Encoding: 8bit 22, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3RGjT30001945 ==============================End of - Headers==============================
I should have also mentioned that we have a printer that we purchased that prints directly onto the painted end of a microscope slide. It is completely solvent resistant. The one we have is particular about the brand of slide we use, but my understanding is that when Thermo-Fisher bought the product line from the original designers, T-F re-worked it so that it was not so fussy. This works well for the 15,000+ histology slides that my University service lab does per year.
We chose this product (Accuplace PSLIM) because it was the least expensive direct slide labeling device available at the time and our tests of solvent-resistant stick-on labels left us unimpressed.
The product is now distributed exclusively by Thermo-Fisher (at least in the US). http://www.thermoscientific.com/ecomm/servlet/productsdetail?productId=11962242&groupType=PRODUCT&searchType=0&storeId=11152
I have no commercial interest in this product. Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cell Biology & Anatomy, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
-----Original Message----- X-from: michael-at-shaffer.net [mailto:michael-at-shaffer.net] Sent: Wednesday, April 27, 2011 9:17 AM To: Cromey, Douglas W - (dcromey)
I was wondering what others may be using to make their labels for glass slides permanent and insoluble to ultrasonic cleaning in ETOH. I've seen some slides where someone had labeled with a Sharpie, then applied something akin to clear nail polish (. except that I'd like to use something similar for covering a tape label). Whatever your method, would it be too much to ask that it also hold up to acetone?
TIA :o) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland SEM-MLA Calendar: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
==============================Original Headers============================== 6, 27 -- From michael-at-shaffer.net Wed Apr 27 11:16:56 2011 6, 27 -- Received: from smtprelay.hostedemail.com (smtprelay0004.hostedemail.com [216.40.44.4]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RGGu4K004116 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 11:16:56 -0500 6, 27 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 6, 27 -- by smtprelay03.hostedemail.com (Postfix) with SMTP id 519BABC5A78 6, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 16:16:56 +0000 (UTC) 6, 27 -- X-Panda: scanned! 6, 27 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574 6, 27 -- X-Filterd-Recvd-Size: 1414 6, 27 -- Received: from shaffer (mshaffer.creait.mun.ca [134.153.131.207]) 6, 27 -- (Authenticated sender: michael-at-shaffer.net) 6, 27 -- by omf02.hostedemail.com (Postfix) with ESMTP 6, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 16:16:55 +0000 (UTC) 6, 27 -- From: "michael shaffer" {michael-at-shaffer.net} 6, 27 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 6, 27 -- Subject: making slide labels permanent 6, 27 -- Date: Wed, 27 Apr 2011 13:46:54 -0230 6, 27 -- Message-ID: {004501cc04f6$86d78940$94869bc0$-at-net} 6, 27 -- MIME-Version: 1.0 6, 27 -- Content-Type: text/plain; 6, 27 -- charset="iso-8859-1" 6, 27 -- X-Mailer: Microsoft Office Outlook 12.0 6, 27 -- Thread-Index: AcwE51huxqC4s4xVSF2Kz//2As/RWw== 6, 27 -- Content-Language: en-ca 6, 27 -- Content-Transfer-Encoding: 8bit 6, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3RGGu4K004116 ==============================End of - Headers==============================
==============================Original Headers============================== 22, 47 -- From dcromey-at-email.arizona.edu Wed Apr 27 11:54:18 2011 22, 47 -- Received: from TX2EHSOBE003.bigfish.com (tx2ehsobe002.messaging.microsoft.com [65.55.88.12]) 22, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RGsHY7020421 22, 47 -- for {Microscopy-at-Microscopy.Com} ; Wed, 27 Apr 2011 11:54:17 -0500 22, 47 -- Received: from mail25-tx2-R.bigfish.com (10.9.14.252) by 22, 47 -- TX2EHSOBE003.bigfish.com (10.9.40.23) with Microsoft SMTP Server id 22, 47 -- 14.1.225.8; Wed, 27 Apr 2011 16:54:17 +0000 22, 47 -- Received: from mail25-tx2 (localhost.localdomain [127.0.0.1]) by 22, 47 -- mail25-tx2-R.bigfish.com (Postfix) with ESMTP id 238031C702A7; Wed, 27 Apr 22, 47 -- 2011 16:54:17 +0000 (UTC) 22, 47 -- X-SpamScore: -24 22, 47 -- X-BigFish: VS-24(zz9371O62a3K7f0R542Mzz1202hzz8275bh8275chz32i87h2a8h668h839h63h) 22, 47 -- X-Spam-TCS-SCL: 2:0 22, 47 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:va3diahub019.RED001.local;RD:smtp801.microsoftonline.com;EFVD:NLI 22, 47 -- X-FB-DOMAIN-IP-MATCH: fail 22, 47 -- Received: from mail25-tx2 (localhost.localdomain [127.0.0.1]) by mail25-tx2 22, 47 -- (MessageSwitch) id 1303923256649220_29081; Wed, 27 Apr 2011 16:54:16 +0000 22, 47 -- (UTC) 22, 47 -- Received: from TX2EHSMHS026.bigfish.com (unknown [10.9.14.244]) by 22, 47 -- mail25-tx2.bigfish.com (Postfix) with ESMTP id 98BE1107804E; Wed, 27 Apr 2011 22, 47 -- 16:54:16 +0000 (UTC) 22, 47 -- Received: from va3diahub019.RED001.local (65.55.171.153) by 22, 47 -- TX2EHSMHS026.bigfish.com (10.9.99.126) with Microsoft SMTP Server (TLS) id 22, 47 -- 14.1.225.8; Wed, 27 Apr 2011 16:54:16 +0000 22, 47 -- Received: from VA3DIAXVS211.RED001.local ([10.32.56.197]) by 22, 47 -- va3diahub019.RED001.local ([10.32.21.19]) with mapi; Wed, 27 Apr 2011 22, 47 -- 09:54:10 -0700 22, 47 -- From: "Cromey, Douglas W - (dcromey)" {dcromey-at-email.arizona.edu} 22, 47 -- To: "michael-at-shaffer.net" {michael-at-shaffer.net} 22, 47 -- CC: "Microscopy listserv (Microscopy-at-Microscopy.Com)" 22, 47 -- {Microscopy-at-Microscopy.Com} 22, 47 -- Date: Wed, 27 Apr 2011 09:53:09 -0700 22, 47 -- Subject: RE: [Microscopy] making slide labels permanent 22, 47 -- Thread-Topic: [Microscopy] making slide labels permanent 22, 47 -- Thread-Index: AcwE9p7ndGntwvQVQBa0+TbbTfKN1wABBa8w 22, 47 -- Message-ID: {33EA1C13F421004C924AD94E7BF09A7D049AC13812-at-VA3DIAXVS211.RED001.local} 22, 47 -- References: {201104271617.p3RGHMHe004858-at-ns.microscopy.com} 22, 47 -- In-Reply-To: {201104271617.p3RGHMHe004858-at-ns.microscopy.com} 22, 47 -- Accept-Language: en-US 22, 47 -- Content-Language: en-US 22, 47 -- X-MS-Has-Attach: 22, 47 -- X-MS-TNEF-Correlator: 22, 47 -- acceptlanguage: en-US 22, 47 -- Content-Type: text/plain; charset="iso-8859-1" 22, 47 -- MIME-Version: 1.0 22, 47 -- Content-Transfer-Encoding: 8bit 22, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3RGsHY7020421 ==============================End of - Headers==============================
Thanks to those who have responded, but I should've also mentioned that I don't want anyone here relying on interpreting someone else's script (student come and they go, and I cannot read half of them), so it does need to be a labeling system.
We have a Brother P-Touch labeler, but I wouldn't have considered the adhesive to survive many ETOH usonic bathes (but I can put it to the test). Also because it would be labeling thousands of polished acrylic blocks, I can also consider a different labeler, because I am wanting to archive these sections and hope they last.
TIAA :o) ~~~~~~~~~~~ previous query ~~~~~~~~~~~~ I was wondering what others may be using to make their labels for glass slides permanent and insoluble to ultrasonic cleaning in ETOH. I’ve seen some slides where someone had labeled with a Sharpie, then applied something akin to clear nail polish (… except that I’d like to use something similar for covering a tape label). Whatever your method, would it be too much to ask that it also hold up to acetone?
TIA :o) ~~~~~~~~~~~~~~~~~~~~~~~~~ cheerios, michael shaffer :o) SEM-MLA Research Coordinator Bruneau Centre for Research & Innovation Memorial University St. John's, Newfoundland SEM-MLA Calendar: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
==============================Original Headers============================== 8, 27 -- From michael-at-shaffer.net Wed Apr 27 12:21:27 2011 8, 27 -- Received: from smtprelay.hostedemail.com (smtprelay0160.hostedemail.com [216.40.44.160]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RHLRuq004539 8, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 12:21:27 -0500 8, 27 -- Received: from filter.hostedemail.com (ff-bigip1 [10.5.19.254]) 8, 27 -- by smtprelay07.hostedemail.com (Postfix) with SMTP id 3F62ADDD096 8, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 17:21:27 +0000 (UTC) 8, 27 -- X-Panda: scanned! 8, 27 -- X-Session-Marker: 6D69636861656C40736861666665722E6E6574 8, 27 -- X-Filterd-Recvd-Size: 2073 8, 27 -- Received: from shaffer (mshaffer.creait.mun.ca [134.153.131.207]) 8, 27 -- (Authenticated sender: michael-at-shaffer.net) 8, 27 -- by omf08.hostedemail.com (Postfix) with ESMTP 8, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 17:21:26 +0000 (UTC) 8, 27 -- From: "michael shaffer" {michael-at-shaffer.net} 8, 27 -- To: "MSA Microscopy list" {Microscopy-at-microscopy.com} 8, 27 -- Subject: making slide labels permanent (II) 8, 27 -- Date: Wed, 27 Apr 2011 14:51:25 -0230 8, 27 -- Message-ID: {007501cc04ff$8a203580$9e60a080$-at-net} 8, 27 -- MIME-Version: 1.0 8, 27 -- Content-Type: text/plain; 8, 27 -- charset="iso-8859-1" 8, 27 -- X-Mailer: Microsoft Office Outlook 12.0 8, 27 -- Thread-Index: AcwE51huxqC4s4xVSF2Kz//2As/RWw== 8, 27 -- Content-Language: en-ca 8, 27 -- Content-Transfer-Encoding: 8bit 8, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3RHLRuq004539 ==============================End of - Headers==============================
Research Assistant/CAMCOR Laboratory Manager Posting: 11114 Location: Eugene Closed: Open Until Filled
The Laboratory Manager provides professional technical management and service to support CAMCOR shared technical laboratories and assets and provides essential specific technical services to a number of CAMCOR analytical laboratories. The Lab Manager provides over sight, supervision and maintenance for CAMCOR shared semi-conductor labs, optical/electrical characterization and sample preparation labs and specific technical sample preparation and instrument operation for several of the laboratories including the NanoFabrication and MicroAnalytical facilities. The Lab Manager will provide supervision and instruction for students using the shared laboratories, optical microscopes, computers, scanners and other shared equipment. The Lab Manager provides computer backup and file sharing maintenance and support for all facilities. The Lab Manager will work closely with the CAMCOR Office Manager and provide back-up assistance as needed to ensure that all necessary operations of the CAMCOR lab facilities are managed.
Qualifications: Bachelor's degree in a science related field and three years of experience in supervision or lead work of laboratory management staff and students.
This position will be hired at a .40 FTE with the possibility of an increase upwards to 1.0 FTE with growth of CAMCOR. For complete application instructions, see job page at: hr.uoregon.edu/jobs.
EO/AA/ADA institution committed to cultural diversity.
==============================Original Headers============================== 7, 22 -- From donovan-at-uoregon.edu Wed Apr 27 15:40:49 2011 7, 22 -- Received: from smtp.uoregon.edu (oh-mserv4.uoregon.edu [128.223.142.147]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3RKem28027275 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 15:40:49 -0500 7, 22 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 7, 22 -- (authenticated bits=0) 7, 22 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id p3RKellP010980 7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 7, 22 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 13:40:48 -0700 7, 22 -- Message-Id: {201104272040.p3RKellP010980-at-smtp.uoregon.edu} 7, 22 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 7, 22 -- Date: Wed, 27 Apr 2011 13:40:40 -0700 7, 22 -- To: microscopy-at-microscopy.com 7, 22 -- From: John Donovan {donovan-at-uoregon.edu} 7, 22 -- Subject: University of Oregon- CAMCOR Research Assistant/Lab Manager 7, 22 -- Mime-Version: 1.0 7, 22 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 22 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.2.15,1.0.148,0.0.0000 7, 22 -- definitions=2011-04-27_07:2011-04-27,2011-04-27,1970-01-01 signatures=0 7, 22 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=15 7, 22 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 7, 22 -- reason=mlx engine=6.0.2-1012030000 definitions=main-1104270143 ==============================End of - Headers==============================
We have a question related to rations of elements in a spectrum quant.
A student was measure Cu, Zn, Sn and Se in a sample. He had vastly different cation ratios depending on whether he included Se or did not include Se in the quantization of a single spectrum. He expected that adding an element to the quantization would not change the ratios of the other elements when analyzing the same spectra.
He used an accelerating voltage of 25 kV and a piece of Cu tape (single sided) for quant optimization. He had a high count rate (~5 kcps) and recorded data for 120s. After recording the data, he specified only the cations for one quantization report and then included Se in the next quantization. He was using the Kalpha lines of Cu and Zn and the Lalpha lines of Sn. There is no overlap in the emission peaks. See the attached data. (He accidentally included Ge in the first data set, even though it was not present, but assume this would not significantly affect the results as the Kalpha of Ge also does not overlap and its concentration was very low.)
Element Weight% Atomic%
Cu K 10.13 12.72 Zn K 6.88 8.39 Ge K 0.19 0.21 Se L 68.23 68.89 Sn L 14.56 9.78
Totals 100.00
Element Weight% Atomic%
Cu K 34.58 43.32 Zn K 23.38 28.48 Sn L 42.04 28.20
Totals 100.00
Assuming no overlap in the emission peaks, do relative amounts change when more elements are specified to be fit? Should he expect the accuracy of fits to be worse when specifying more elements? He is mostly concerned with the ratios of elements (say, Zn/Sn), and it seems these ratios are changing when he specify more elements.
Any explanation of the results would be appreciated.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 15, 28 -- From dsherman-at-purdue.edu Wed Apr 27 21:34:43 2011 15, 28 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3S2YheF017533 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 21:34:43 -0500 15, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 15, 28 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p3S2YgmO011760 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 22:34:43 -0400 15, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.138]) by WPPEXHUB01F.purdue.lcl 15, 28 -- ([::1]) with mapi; Wed, 27 Apr 2011 22:34:42 -0400 15, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 15, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 15, 28 -- Date: Wed, 27 Apr 2011 22:34:41 -0400 15, 28 -- Subject: Question about EDS results. 15, 28 -- Thread-Topic: Question about EDS results. 15, 28 -- Thread-Index: AcwFTNNxEB/ITv5+jE6/CKC0vjAZYg== 15, 28 -- Message-ID: {C9DE4A81.E0B6%dsherman-at-purdue.edu} 15, 28 -- Accept-Language: en-US 15, 28 -- Content-Language: en-US 15, 28 -- X-MS-Has-Attach: 15, 28 -- X-MS-TNEF-Correlator: 15, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 15, 28 -- acceptlanguage: en-US 15, 28 -- Content-Type: text/plain; charset="us-ascii" 15, 28 -- MIME-Version: 1.0 15, 28 -- X-PMX-Version: 5.5.9.388399 15, 28 -- X-PerlMx-Virus-Scanned: Yes 15, 28 -- Content-Transfer-Encoding: 8bit 15, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3S2YheF017533 ==============================End of - Headers==============================
For standardless, normalized EDS data with missing elements and an unnecessarily high voltage, the ratios of Cu-Zn-Sn are actually pretty good: 12.7-8.4-9.8 vs 43.3-28.5-28.2, so roughly 1.5-1-1. Given the errors involved, that is probably about as good as you're going to get. Standards aren't optional for "quantitation." Normalizing masks the errors involved. Missing elements affect the matrix corrections in unpredictable ways. High accelerating voltages make this worse by making X-ray absorption greater. Doing quicky semi-quant EDS will yield that degree of error -- it is generous to call that "quantitation." Actual quantitative EDS analysis is what your student needs to do, or better yet, greater accuracy and precision can be gained using quantitative EMPA-WDS instead.
Best, Ellery
Ellery Frahm, Ph.D. Research Associate, Department of Geology & Geophysics Manager & Principal Analyst, Electron Microprobe Laboratory University of Minnesota - Twin Cities campus Lab website: http://probelab.geo.umn.edu/ Personal: http://web.mac.com/elleryfrahm/
==============================Original Headers============================== 9, 20 -- From frah0010-at-umn.edu Wed Apr 27 22:15:50 2011 9, 20 -- Received: from mta-w3.tc.umn.edu (mta-w3.tc.umn.edu [134.84.119.32]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3S3FoOb001799 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2011 22:15:50 -0500 9, 20 -- Received: from [10.0.1.14] (c-75-72-178-217.hsd1.mn.comcast.net [75.72.178.217]) 9, 20 -- by mta-w3.tc.umn.edu (UMN smtpd) with ESMTP 9, 20 -- Wed, 27 Apr 2011 22:15:50 -0500 (CDT) 9, 20 -- X-Umn-Remote-Mta: [N] c-75-72-178-217.hsd1.mn.comcast.net [75.72.178.217] #+TS+AU+HN 9, 20 -- Content-Type: text/plain; charset=us-ascii 9, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) 9, 20 -- Subject: Re: [Microscopy] Question about EDS results. 9, 20 -- From: Ellery Frahm {frah0010-at-umn.edu} 9, 20 -- In-Reply-To: {201104280238.p3S2cuQr024349-at-ns.microscopy.com} 9, 20 -- Date: Wed, 27 Apr 2011 22:15:49 -0500 9, 20 -- Message-Id: {D80A9BEC-3D7F-4E25-A672-B72FC5270F5F-at-umn.edu} 9, 20 -- References: {201104280238.p3S2cuQr024349-at-ns.microscopy.com} 9, 20 -- To: Debra M Sherman {dsherman-at-purdue.edu} , microscopy-at-microscopy.com 9, 20 -- X-Mailer: Apple Mail (2.1082) 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3S3FoOb001799 ==============================End of - Headers==============================
The presence or absence of Se can have an appreciable effect on the ZAF correction especially if it is present in the sample in significant quantities.
Se has ionization energies at 11.2 keV (Ka), 12.5 keV (Kb) & 1.38 keV (L). I would expect the following:
Sn K lines (25.2, 28.6 keV) to be absorbed by Se K edges. Se K edges to fluoresce Cu K (8.0 & 8.8 keV) and Zn K (8.6 & 9.6 keV) Se L lines to absorb Sn L lines (3.4, 3.7 keV).
Notice that Se tends to absorb Sn-related X-rays, but increase the Cu & Zn X-ray intensities, i.e. the ZAF correction for Se has opposite effects on the Sn relative to the Cu/Zn.
Looking at the first set of data the elemental ratio Cu:Zn:Sn is about 6:4:5. With the Se inclusion this becomes 6:4:4, which is not that significant a change. This looks fine to me.
Yours, Jon
} Hi all, } } We have a question related to rations of elements in a spectrum quant. } } A student was measure Cu, Zn, Sn and Se in a sample. He had vastly } different cation ratios depending on whether he included Se or did not } include Se in the quantization of a single spectrum. He expected that } adding an element to the quantization would not change the ratios of the } other elements when analyzing the same spectra. } } He used an accelerating voltage of 25 kV and a piece of Cu tape (single } sided) for quant optimization. He had a high count rate (~5 kcps) and } recorded data for 120s. After recording the data, he specified only the } cations for one quantization report and then included Se in the next } quantization. He was using the Kalpha lines of Cu and Zn and the Lalpha } lines of Sn. There is no overlap in the emission peaks. See the attached } data. (He accidentally included Ge in the first data set, } even though it was not present, but assume this would not } significantly affect the results as the Kalpha of Ge also does not } overlap and its concentration was very low.) } } } Element Weight% Atomic% } } Cu K 10.13 12.72 } Zn K 6.88 8.39 } Ge K 0.19 0.21 } Se L 68.23 68.89 } Sn L 14.56 9.78 } } Totals 100.00 } } } Element Weight% Atomic% } } Cu K 34.58 43.32 } Zn K 23.38 28.48 } Sn L 42.04 28.20 } } Totals 100.00 } } Assuming no overlap in the emission peaks, do relative amounts change } when more elements are specified to be fit? Should he expect the } accuracy of fits to be worse when specifying more elements? He is mostly } concerned with the ratios of elements (say, Zn/Sn), and it seems these } ratios are changing when he specify more elements. } } Any explanation of the results would be appreciated. } } Debby } } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy } } } } } } ==============================Original } Headers============================== 15, 28 -- From dsherman-at-purdue.edu } Wed Apr 27 21:34:43 2011 15, 28 -- Received: from } mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) } 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3S2YheF017533 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr } 2011 21:34:43 -0500 15, 28 -- Received: from WPPEXHUB01F.purdue.lcl } (wppexhub01f.itap.purdue.edu [172.21.6.90]) 15, 28 -- by } mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with } ESMTP id p3S2YgmO011760 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, } 27 Apr 2011 22:34:43 -0400 15, 28 -- Received: from VPEXCH04.purdue.lcl } ([169.254.2.138]) by WPPEXHUB01F.purdue.lcl 15, 28 -- ([::1]) with mapi; } Wed, 27 Apr 2011 22:34:42 -0400 15, 28 -- From: "Sherman, Debra M" } {dsherman-at-purdue.edu} 15, 28 -- To: "message to: MSA list" } {microscopy-at-microscopy.com} 15, 28 -- Date: Wed, 27 Apr 2011 22:34:41 } -0400 15, 28 -- Subject: Question about EDS results. 15, 28 -- } Thread-Topic: Question about EDS results. 15, 28 -- Thread-Index: } AcwFTNNxEB/ITv5+jE6/CKC0vjAZYg== 15, 28 -- Message-ID: } {C9DE4A81.E0B6%dsherman-at-purdue.edu} 15, 28 -- Accept-Language: en-US 15, } 28 -- Content-Language: en-US 15, 28 -- X-MS-Has-Attach: 15, 28 -- } X-MS-TNEF-Correlator: 15, 28 -- user-agent: } Microsoft-Entourage/13.8.0.101117 15, 28 -- acceptlanguage: en-US 15, 28 } -- Content-Type: text/plain; charset="us-ascii" 15, 28 -- MIME-Version: } 1.0 15, 28 -- X-PMX-Version: 5.5.9.388399 15, 28 -- } X-PerlMx-Virus-Scanned: Yes 15, 28 -- Content-Transfer-Encoding: 8bit 15, } 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p3S2YheF017533 ==============================End of } - Headers==============================
==============================Original Headers============================== 9, 28 -- From jsb43-at-hermes.cam.ac.uk Thu Apr 28 03:42:19 2011 9, 28 -- Received: from ppsw-50.csi.cam.ac.uk (ppsw-50.csi.cam.ac.uk [131.111.8.150]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3S8gJwD022625 9, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 03:42:19 -0500 9, 28 -- X-Cam-AntiVirus: no malware found 9, 28 -- X-Cam-SpamDetails: not scanned 9, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 9, 28 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:59362) 9, 28 -- by ppsw-50.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.157]:25) 9, 28 -- with esmtpa (EXTERNAL:jsb43) id 1QFMnh-000618-s5 (Exim 4.72) 9, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 28 Apr 2011 09:42:17 +0100 9, 28 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk) 9, 28 -- with local (PRAYER:jsb43) id 1QFMnh-00081j-OL (Exim 4.67) 9, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Thu, 28 Apr 2011 09:42:17 +0100 9, 28 -- Received: from [128.232.229.159] by webmail.hermes.cam.ac.uk 9, 28 -- with HTTP (Prayer-1.3.3); 28 Apr 2011 09:42:17 +0100 9, 28 -- Date: 28 Apr 2011 09:42:17 +0100 9, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 9, 28 -- To: dsherman-at-purdue.edu 9, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 9, 28 -- Subject: Re: Question about EDS results. 9, 28 -- Message-ID: {Prayer.1.3.3.1104280942170.15248-at-hermes-2.csi.cam.ac.uk} 9, 28 -- In-Reply-To: {201104280240.p3S2eYSW027084-at-ns.microscopy.com} 9, 28 -- References: {201104280240.p3S2eYSW027084-at-ns.microscopy.com} 9, 28 -- X-Mailer: Prayer v1.3.3 9, 28 -- Mime-Version: 1.0 9, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 9, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
} Date: Thu, 28 Apr 2011 06:37:16 -0700 } From: Georgianne Ciraolo {Georgianne.Ciraolo-at-cchmc.org} } } realname - Georgianne Ciraolo } Email - Georgianne.Ciraolo-at-cchmc.org } ORGANIZATION - Cincinnati Children's Hospital Dept. of Pathology } EDUCATION - Graduate College } LOCATION - Cincinnati Ohio USA } SUBJECT_OF_QUESTION - fixation of mouse femurs for transmission } electrom microscopy } QUESTION - Does anyone have a protocol for fixation of bone for TEM } ? Do I need to decal or is there some other method? Any } suggestions would be greatly appreciated. } } Thanks } } Georgianne Ciraolo -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 27 -- From oshel1pe-at-cmich.edu Thu Apr 28 09:16:27 2011 1, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SEGRUb012632 1, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 09:16:27 -0500 1, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 1, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p3SEGKer012026 1, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 10:16:27 -0400 1, 27 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 1, 27 -- Thu, 28 Apr 2011 10:15:40 -0400 1, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Thu, 28 Apr 1, 27 -- 2011 10:15:40 -0400 1, 27 -- MIME-Version: 1.0 1, 27 -- Message-ID: {a06240802c9df26897b4f-at-[141.209.160.249]} 1, 27 -- Date: Thu, 28 Apr 2011 10:15:38 -0400 1, 27 -- To: {Microscopy-at-microscopy.com} 1, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 27 -- Subject: Fwd: Ask-A-Microscopist bone methods for TEM 1, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 27 -- X-Originating-IP: [141.209.160.249] 1, 27 -- X-OriginalArrivalTime: 28 Apr 2011 14:15:40.0840 (UTC) FILETIME=[C10FFA80:01CC05AE] 1, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 27 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 1, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 27 -- X-CanItPRO-Stream: default 1, 27 -- X-Canit-Stats-ID: 02EB2grEm - fdeb5bc34a4a - 20110428 1, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
We have a researcher who wants to do TEM on motor end plates from a mouse diaphragm . Is there a good way to localize NMJ before we process the tissue for TEM? Any advice will be greatly appreciated.
Thanks, Maya Yankova, M.Sc. Research Assistant 3 Central Electron Microscopy Facility Department of Molecular, Microbial & Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3305 ph: 860.679.2395 yankova-at-neuron.uchc.edu
==============================Original Headers============================== 10, 25 -- From prvs=10926ea75f=yankova-at-neuron.uchc.edu Thu Apr 28 10:23:19 2011 10, 25 -- Received: from uchc.edu (itiron2.uchc.edu [155.37.201.27]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SFNJgv029971 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 10:23:19 -0500 10, 25 -- Received: from ([10.91.15.93]) 10, 25 -- by itiron2.uchc.edu with ESMTP with TLS id CNJDJN1.5452318; 10, 25 -- Thu, 28 Apr 2011 11:23:16 -0400 10, 25 -- Received: from NSO-ITEXC-CCR04.uchc.net ([10.91.15.114]) by 10, 25 -- NSO-ITEXC-HT03.uchc.net ([::1]) with mapi; Thu, 28 Apr 2011 11:23:15 -0400 10, 25 -- From: "Yankova,Maya" {Yankova-at-neuron.uchc.edu} 10, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 25 -- Date: Thu, 28 Apr 2011 11:23:14 -0400 10, 25 -- Subject: TEM on motor end plates 10, 25 -- Thread-Topic: TEM on motor end plates 10, 25 -- Thread-Index: AQHMBbgxl7G/AbcWvUaVSsGJTymPIw== 10, 25 -- Message-ID: {8E7BE6D1E258C7449A99EAA21E6B9A5FAE8A248FFF-at-NSO-ITEXC-CCR04.uchc.net} 10, 25 -- Accept-Language: en-US 10, 25 -- Content-Language: en-US 10, 25 -- X-MS-Has-Attach: 10, 25 -- X-MS-TNEF-Correlator: 10, 25 -- acceptlanguage: en-US 10, 25 -- Content-Type: text/plain; charset="iso-8859-1" 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3SFNJgv029971 ==============================End of - Headers==============================
Hello All, I am a relatively new user and only operator of our Hitachi-7100 TEM. I went to use the instrument for the first time in a month and was not able to find the beam. I have bright illumination but no focussed beam. I have backed out the apertures and specimen holder to adjust the Gun alignment to get the brightest illumination at the largest spot size. There looks to be somewhat of a beam at the top of the screen but I am unable to get it to the centre without losing it all together.
I know this problem is well below the level of discussion on this ListServ so if you have any suggestions please feel free to contact me off line.
Thank you all for your suggestions. I hesitate to tell you what the problem was but I guess its a learning experience...the shutter was closed.
Tracy
Hello All, I am a relatively new user and only operator of our Hitachi-7100 TEM. I went to use the instrument for the first time in a month and was not able to find the beam. I have bright illumination but no focussed beam. I have backed out the apertures and specimen holder to adjust the Gun alignment to get the brightest illumination at the largest spot size. There looks to be somewhat of a beam at the top of the screen but I am unable to get it to the centre without losing it all together.
I know this problem is well below the level of discussion on this ListServ so if you have any suggestions please feel free to contact me off line.
Hi Georgianne, As usual, the best way to fix a mouse bone is by perfusion. 2.5% glut works fine. If perfusion is not possible, just go as with any other tissue: put a small piece of it in fixative as soon as possible. Splitting a bone can help for better (faster) fixation. If bone was split, then length is of no importance, it could be several mm. Main difference from other tissues: fix bone longer; 2-4 hrs at room temperature, or overnight at 4 Celsius. Only after fixation you can demineralize a bone, if you need to (if you are looking not for mineral distribution, but are interested in collagen, osteocytes, etc.) Good luck, Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] } Sent: Thursday, April 28, 2011 9:17 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Fwd: Ask-A-Microscopist bone methods for TEM } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } } Date: Thu, 28 Apr 2011 06:37:16 -0700 } } From: Georgianne Ciraolo {Georgianne.Ciraolo-at-cchmc.org} } } } } realname - Georgianne Ciraolo } } Email - Georgianne.Ciraolo-at-cchmc.org } } ORGANIZATION - Cincinnati Children's Hospital Dept. of Pathology } } EDUCATION - Graduate College } } LOCATION - Cincinnati Ohio USA } } SUBJECT_OF_QUESTION - fixation of mouse femurs for transmission } } electrom microscopy } } QUESTION - Does anyone have a protocol for fixation of bone for TEM } } ? Do I need to decal or is there some other method? Any } } suggestions would be greatly appreciated. } } } } Thanks } } } } Georgianne Ciraolo } -- } *********************************************************************** } **************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************** } ***************** } } ==============================Original } Headers============================== } 1, 27 -- From oshel1pe-at-cmich.edu Thu Apr 28 09:16:27 2011 } 1, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) } 1, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p3SEGRUb012632 } 1, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 09:16:27 } -0500 } 1, 27 -- Received: from egatea.central.cmich.local ([141.209.15.74]) } 1, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP } id p3SEGKer012026 } 1, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 10:16:27 } -0400 } 1, 27 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by } egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); } 1, 27 -- Thu, 28 Apr 2011 10:15:40 -0400 } 1, 27 -- Received: from [141.209.160.249] (141.209.160.249) by } cas1.central.cmich.local } 1, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id } 14.1.270.1; Thu, 28 Apr } 1, 27 -- 2011 10:15:40 -0400 } 1, 27 -- MIME-Version: 1.0 } 1, 27 -- Message-ID: {a06240802c9df26897b4f-at-[141.209.160.249]} } 1, 27 -- Date: Thu, 28 Apr 2011 10:15:38 -0400 } 1, 27 -- To: {Microscopy-at-microscopy.com} } 1, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} } 1, 27 -- Subject: Fwd: Ask-A-Microscopist bone methods for TEM } 1, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed } 1, 27 -- X-Originating-IP: [141.209.160.249] } 1, 27 -- X-OriginalArrivalTime: 28 Apr 2011 14:15:40.0840 (UTC) } FILETIME=[C10FFA80:01CC05AE] } 1, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, } default) } 1, 27 -- X-Spam-Score: -4.20 () [Hold at 6.00] } L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) } 1, 27 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; } city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=- } 84.8473; metrocode=513; areacode=989; } http://maps.google.com/maps?q=43.5647,-84.8473&z=6 } 1, 27 -- X-CanItPRO-Stream: default } 1, 27 -- X-Canit-Stats-ID: 02EB2grEm - fdeb5bc34a4a - 20110428 } 1, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on } 141.209.20.25 } ==============================End of - } Headers==============================
==============================Original Headers============================== 4, 29 -- From DusevichV-at-umkc.edu Thu Apr 28 14:30:35 2011 4, 29 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SJUZeJ018426 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 14:30:35 -0500 4, 29 -- X-IronPort-Anti-Spam-Filtered: true 4, 29 -- X-IronPort-Anti-Spam-Result: AiEBABrAuU2Dl3nN/2dsb2JhbACYE41yd70KiG+FdgSGCZZ6 4, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 4, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 28 Apr 2011 14:30:34 -0500 4, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.42]) by 4, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Thu, 28 Apr 2011 4, 29 -- 14:30:34 -0500 4, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 4, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 29 -- Date: Thu, 28 Apr 2011 14:30:35 -0500 4, 29 -- Subject: RE: [Microscopy] Fwd: Ask-A-Microscopist bone methods for TEM 4, 29 -- Thread-Topic: [Microscopy] Fwd: Ask-A-Microscopist bone methods for TEM 4, 29 -- Thread-Index: AcwFrwHD7Nn+OhMsQ7a2iVmg6p7A+gAKZvqQ 4, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3AE16852851-at-UM-EMAIL06.um.umsystem.edu} 4, 29 -- References: {201104281417.p3SEHM3G013392-at-ns.microscopy.com} 4, 29 -- In-Reply-To: {201104281417.p3SEHM3G013392-at-ns.microscopy.com} 4, 29 -- Accept-Language: en-US 4, 29 -- Content-Language: en-US 4, 29 -- X-MS-Has-Attach: 4, 29 -- X-MS-TNEF-Correlator: 4, 29 -- acceptlanguage: en-US 4, 29 -- Content-Type: text/plain; charset="us-ascii" 4, 29 -- MIME-Version: 1.0 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3SJUZeJ018426 ==============================End of - Headers==============================
What an emotional day- tears to laughter! Thank you Jan.
Tracy
} } } Jan Leunissen - Aurion {leunissen-at-aurion.nl} 4/28/2011 12:03 pm } } } I should tell you what it was, so you can smile as well:
I was in Vienna last year to do some tests. And you should know that I am not actively involved with an institute, out of practice somewhat (in my defense, weak... but still). Had only worked with the Morgagni once or twice, trying to do all well along the instructions I had received. So ... switched on HT, started up the filament... nothing. Checked if I had made a mistake ... no, didn't seem like that, HT was on, we had a filament current as well as a beam current, but the TEM chamber was dark as.
I thought: I'll be b...d if I have to ask for help, after all those years being an electron microscopist.... And of course there is also that feeling of looking silly. After 10 minutes of trying, I gave up and was about to get help. Then I turned the room lights on... and there was the reason for not getting any beam: the cover was still on the glass pane of the viewing chamber! I sat there laughing for a few minutes and felt so silly. Decided to tell the friendly crew in Vienna anyway, but they still tease me with it, it was so funny.
Well, hope that made you smile too.
Cheers
Jan
On 29/04/2011, at 6:45 AM, Tracy.Lawrence-at-inspection.gc.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------- } } Thank you all for your suggestions. I hesitate to tell you what the } problem was but I guess its a learning experience...the shutter was } closed. } } Tracy } } } Hello All, } I am a relatively new user and only operator of our Hitachi-7100 TEM.
OK, show of hands; how many of you have done this, even briefly?
Tracy, we all have stories like yours! Welcome to the club.
Aloha, Tina
} } } } Jan Leunissen - Aurion {leunissen-at-aurion.nl} 4/28/2011 12:03 pm } } } } } I should tell you what it was, so you can smile as well: } } I was in Vienna last year to do some tests. And you should know that I } am not actively involved with an institute, out of practice somewhat (in } my defense, weak... but still). } Had only worked with the Morgagni once or twice, trying to do all well } along the instructions I had received. So ... switched on HT, started up } the filament... nothing. } Checked if I had made a mistake ... no, didn't seem like that, HT was } on, we had a filament current as well as a beam current, but the TEM } chamber was dark as. } } I thought: I'll be b...d if I have to ask for help, after all those } years being an electron microscopist.... And of course there is also } that feeling of looking silly. } After 10 minutes of trying, I gave up and was about to get help. Then I } turned the room lights on... and there was the reason for not getting } any beam: the cover was still on the glass pane of the viewing chamber! } I sat there laughing for a few minutes and felt so silly. Decided to } tell the friendly crew in Vienna anyway, but they still tease me with } it, it was so funny.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 5, 22 -- From tina-at-pbrc.hawaii.edu Thu Apr 28 15:04:06 2011 5, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SK45JZ016980 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 15:04:05 -0500 5, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 5, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p3SK44pY002422 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 10:04:04 -1000 (HST) 5, 22 -- Received: from localhost (tina-at-localhost) 5, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p3SK43pY002417 5, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 10:04:03 -1000 (HST) 5, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 5, 22 -- Date: Thu, 28 Apr 2011 10:04:03 -1000 (HST) 5, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 5, 22 -- X-X-Sender: tina-at-b1000 5, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] Re: TEM Alignment 5, 22 -- In-Reply-To: {201104281958.p3SJwSHu003962-at-ns.microscopy.com} 5, 22 -- Message-ID: {Pine.GSO.4.64.1104281001440.1356-at-b1000} 5, 22 -- References: {201104281958.p3SJwSHu003962-at-ns.microscopy.com} 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
As Tina says, welcome - you are now a full, initiated member of the club. My worst happened when I was on vacation and a tech decided that the filament was blown. Getting an enterprising group together, including several academics, including the Unit Director .... Well, suffice to say the Wehnalt was so badly cross threaded when they were done that we had to replace it. While I did not laugh at the time, it proved a very valuable teaching lesson - to me and for me to use.
Since then I prepared a (laminated) check list titled "What to do if the screen is black." It is well used, including by me.
paul -- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
Yep. Even better is when you do this during a demonstration for a class ...
Phil
} OK, show of hands; how many of you have done this, even briefly? } } Tracy, we all have stories like yours! Welcome to the club. } } Aloha, } Tina } } } } } } Jan Leunissen - Aurion {leunissen-at-aurion.nl} 4/28/2011 12:03 pm } } } } } } } I should tell you what it was, so you can smile as well: } } } } I was in Vienna last year to do some tests. And you should know that I } } am not actively involved with an institute, out of practice somewhat (in } } my defense, weak... but still). } } Had only worked with the Morgagni once or twice, trying to do all well } } along the instructions I had received. So ... switched on HT, started up } } the filament... nothing. } } Checked if I had made a mistake ... no, didn't seem like that, HT was } } on, we had a filament current as well as a beam current, but the TEM } } chamber was dark as. } } } } I thought: I'll be b...d if I have to ask for help, after all those } } years being an electron microscopist.... And of course there is also } } that feeling of looking silly. } } After 10 minutes of trying, I gave up and was about to get help. Then I } } turned the room lights on... and there was the reason for not getting } } any beam: the cover was still on the glass pane of the viewing chamber! } } I sat there laughing for a few minutes and felt so silly. Decided to } } tell the friendly crew in Vienna anyway, but they still tease me with } } it, it was so funny. } } **************************************************************************** } * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * } * Biological Electron Microscope Facility * (808) 956-6251 * } * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* } ****************************************************************************
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 29 -- From oshel1pe-at-cmich.edu Thu Apr 28 15:29:09 2011 4, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SKT9Nq019016 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 15:29:09 -0500 4, 29 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p3SKSYXm010627 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 16:29:08 -0400 4, 29 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 4, 29 -- Thu, 28 Apr 2011 16:29:05 -0400 4, 29 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 29 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Thu, 28 Apr 4, 29 -- 2011 16:29:05 -0400 4, 29 -- MIME-Version: 1.0 4, 29 -- Message-ID: {a06240818c9df7e3b0911-at-[141.209.160.249]} 4, 29 -- In-Reply-To: {201104282006.p3SK6pwW024004-at-ns.microscopy.com} 4, 29 -- References: {201104282006.p3SK6pwW024004-at-ns.microscopy.com} 4, 29 -- Date: Thu, 28 Apr 2011 16:29:03 -0400 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 29 -- Subject: Re: [Microscopy] TEM Alignment 4, 29 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 29 -- X-Originating-IP: [141.209.160.249] 4, 29 -- X-OriginalArrivalTime: 28 Apr 2011 20:29:05.0748 (UTC) FILETIME=[EB6DA140:01CC05E2] 4, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 29 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 4, 29 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 29 -- X-CanItPRO-Stream: default 4, 29 -- X-Canit-Stats-ID: 02EB8t8Lf - d9f820353835 - 20110428 4, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Yes indeed, we all do experience such events. When I first joined the faculty of the University of Michigan the Department had just acquired an RCA Model EML TEM. The person who had been responsible for it's purchase had left, and I was put in charge of it. One day I walked into the lab and tried to turn it on, but despite all my best efforts I couldn't get the thing running. Finally, I called the local RCA Service Engineer, Tony Boulet, for help. He showed up, pushed the main power switch a couple of times, then walked around behind the instrument and said, "It helps if you plug it in". It turned out that the darned thing wasn't hard wired into the electrical system, but simply had a power line that plugged into a wall outlet. Somehow or other the plug had come out of the socket and was laying peacefully on the floor. It was so embarrassing that it was funny. I ended up taking Tony out for a steak dinner, and I enjoyed his expert assistance and his friendship for many years thereafter. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
Hi All, I may have everyone beat, so my hand is definitely up. When doing cryo-EM on a Tecnai 12 with the specimen mounted in a Gatan 626 cryostage, one closes the gun valve to load the specimen, which has a cryoshield in place to prevent ice contamination of the grid. After the specimen is in place and the temperature has stabilized, the gun valve is opened, the cryoshield is retracted, and the specimen can be observed. At one time or another I have had the experience of not seeing the beam due to forgetting each of the three items: opening the valve, retracting the shield, and, of course, failing to remove the cover. Yours, Bill
X-from: tina-at-pbrc.hawaii.edu To: William.F.Tivol-at-aero.org
OK, show of hands; how many of you have done this, even briefly?
Tracy, we all have stories like yours! Welcome to the club.
Aloha, Tina
} } } } Jan Leunissen - Aurion {leunissen-at-aurion.nl} 4/28/2011 12:03 pm } } } } } I should tell you what it was, so you can smile as well: } } I was in Vienna last year to do some tests. And you should know that I } am not actively involved with an institute, out of practice somewhat (in } my defense, weak... but still). } Had only worked with the Morgagni once or twice, trying to do all well } along the instructions I had received. So ... switched on HT, started up } the filament... nothing. } Checked if I had made a mistake ... no, didn't seem like that, HT was } on, we had a filament current as well as a beam current, but the TEM } chamber was dark as. } } I thought: I'll be b...d if I have to ask for help, after all those } years being an electron microscopist.... And of course there is also } that feeling of looking silly. } After 10 minutes of trying, I gave up and was about to get help. Then I } turned the room lights on... and there was the reason for not getting } any beam: the cover was still on the glass pane of the viewing chamber! } I sat there laughing for a few minutes and felt so silly. Decided to } tell the friendly crew in Vienna anyway, but they still tease me with } it, it was so funny.
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SNKq3n005977 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 28 Apr 2011 18:20:52 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3SNKqfW005975; Thu, 28 Apr 2011 18:20:52 -0500
Does anyone have any experience of high pressure freezing of whole embryos of invertebrates, other than Drosophila?
I'm aware that this is a fairly wide area, and that there is a lot of Caenorhabditis literature. I'm interested in invertebrates where the embryos are at the upper size limit of being squashed into an HPF planchette.
I've found the nice McDonald and Morphew paper from 1993 on Drosophila and Strongylocentrotus, and the mention in various papers that wasps had been done in Berkeley, but as far as I can tell, all the other invertebrate embryo papers deal with bits of tissue from the embryos. I'm probably missing stuff though... any pointers greatly appreciated.
Thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
Dear Giselle, I have not had experience freezing embryos, but you may want to look at Jan Leunissen's technique of constant-volume freezing, which can work for large volumes. Yours, Bill
X-from: giselle.walker-at-anatomy.otago.ac.nz To: William.F.Tivol-at-aero.org
Does anyone have any experience of high pressure freezing of whole embryos of invertebrates, other than Drosophila?
I'm aware that this is a fairly wide area, and that there is a lot of Caenorhabditis literature. I'm interested in invertebrates where the embryos are at the upper size limit of being squashed into an HPF planchette.
I've found the nice McDonald and Morphew paper from 1993 on Drosophila and Strongylocentrotus, and the mention in various papers that wasps had been done in Berkeley, but as far as I can tell, all the other invertebrate embryo papers deal with bits of tissue from the embryos. I'm probably missing stuff though... any pointers greatly appreciated.
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3T0CeUd006471 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 28 Apr 2011 19:12:40 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p3T0Ce5t006469; Thu, 28 Apr 2011 19:12:40 -0500
Hi Giselle, thanks Bill
We tested tube diameters up to 0.8 mm internal diameter (1.6 mm outer diameter) which worked for yeast and CE. Not sure if they would be ok for more sensitive material as at least for part of the tube diameter the freezing speed may be too low. Impossible to predict, you'd have to give it a go.
Good luck.
Jan
On 29/04/2011, at 11:34 AM, William.F.Tivol-at-aero.org wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Giselle, } I have not had experience freezing embryos, but you may want to } look at Jan Leunissen's technique of constant-volume freezing, which can } work for large volumes. } Yours, } Bill } } } } X-from: giselle.walker-at-anatomy.otago.ac.nz } To: William.F.Tivol-at-aero.org } Date: 04/28/2011 04:27 PM } Subject: [Microscopy] HPF on invertebrate embryos } } } } Does anyone have any experience of high pressure freezing of whole embryos } of invertebrates, other than Drosophila? } } I'm aware that this is a fairly wide area, and that there is a lot of } Caenorhabditis literature. I'm interested in invertebrates where the } embryos are at the upper size limit of being squashed into an HPF } planchette. } } I've found the nice McDonald and Morphew paper from 1993 on Drosophila and } Strongylocentrotus, and the mention in various papers that wasps had been } done in Berkeley, but as far as I can tell, all the other invertebrate } embryo papers deal with bits of tissue from the embryos. I'm probably } missing stuff though... any pointers greatly appreciated. } } Thanks } } Giselle } } } ==============================Original Headers============================== } 12, 46 -- From prvs=0927e9869=William.F.Tivol-at-aero.org Thu Apr 28 18:34:42 2011 } 12, 46 -- Received: from email1-west.aero.org (email1-west.aero.org [130.221.16.26]) } 12, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SNYgwQ022136 } 12, 46 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 18:34:42 -0500 } 12, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 12, 46 -- d=aero.org; i=William.F.Tivol-at-aero.org; q=dns/txt; } 12, 46 -- s=mailhub; t=1304033683; x=1335569683; } 12, 46 -- h=in-reply-to:references:to:mime-version:subject: } 12, 46 -- message-id:date:from; } 12, 46 -- z=In-Reply-To:=20 {201104282327.p3SNR7qE020627-at-ns.microscop } 12, 46 -- y.com} |References:=20 {201104282327.p3SNR7qE020627-at-ns.micr } 12, 46 -- oscopy.com} |To:=20microscopy-at-microscopy.com|MIME-Version: } 12, 46 -- =201.0|Subject:=20Re:=20[Microscopy]=20HPF=20on=20inverte } 12, 46 -- brate=20embryos|Message-ID:=20 {OF4831C922.2118C3CF-ON8825 } 12, 46 -- 7880.00812D17-88257880.0081852F-at-notes.aero.org} |Date:=20T } 12, 46 -- hu,=2028=20Apr=202011=2016:34:39=20-0700|From:=20William } 12, 46 -- =20F=20Tivol=20 {William.F.Tivol-at-aero.org} ; } 12, 46 -- bh=PSqb2d/yL7lk/ccNVpycgVJwqSw4P+lYK5qV/D7bhhg=; } 12, 46 -- b=pbYjCZ1M7M4mLZlQb+tGhBTT9eagGyp0UOeYA4PlYvy01hzLOiqFoJbk } 12, 46 -- SZOWMj+3a6DpU20WIFBXK7pcMDP/+8Ea0b/ku5zkFExhCGBZpZIcERdlx } 12, 46 -- F2EyuGu7+yIMG8n12pRu7iJQ8t9FQWAHX9VZO+WyFSmoV8PkrmEj8Gpy4 } 12, 46 -- A=; } 12, 46 -- From: William F Tivol {William.F.Tivol-at-aero.org} } 12, 46 -- x-SBRS: 2.9 } 12, 46 -- x-SenderGroup: Internal_Relay_hosts } 12, 46 -- X-IronPort-Anti-Spam-Filtered: true } 12, 46 -- X-IronPort-Anti-Spam-Result: AsQEAFn4uU2C3VwSgWdsb2JhbACYSI05FAEBFiYlxlWFdgSGCYhTjis } 12, 46 -- X-IronPort-AV: E=McAfee;i="5400,1158,6330"; a="55352020" } 12, 46 -- X-IronPort-AV: E=Sophos;i="4.64,283,1301900400"; } 12, 46 -- d="scan'208";a="55352020" } 12, 46 -- Received: from agosmtpout.aero.org ([130.221.92.18]) } 12, 46 -- by mailhub1.aero.org with ESMTP; 28 Apr 2011 16:34:42 -0700 } 12, 46 -- In-Reply-To: {201104282327.p3SNR7qE020627-at-ns.microscopy.com} } 12, 46 -- References: {201104282327.p3SNR7qE020627-at-ns.microscopy.com} } 12, 46 -- To: microscopy-at-microscopy.com } 12, 46 -- MIME-Version: 1.0 } 12, 46 -- Subject: Re: [Microscopy] HPF on invertebrate embryos } 12, 46 -- X-KeepSent: 4831C922:2118C3CF-88257880:00812D17; } 12, 46 -- type=4; name=$KeepSent } 12, 46 -- X-Mailer: Lotus Notes Release 8.5.1 September 28, 2009 } 12, 46 -- Message-ID: {OF4831C922.2118C3CF-ON88257880.00812D17-88257880.0081852F-at-notes.aero.org} } 12, 46 -- Date: Thu, 28 Apr 2011 16:34:39 -0700 } 12, 46 -- X-MIMETrack: Serialize by Router on agosmtpout/AeroNet/Aerospace/US(Release 8.5.2 HF250|November } 12, 46 -- 18, 2010) at 04/28/2011 04:34:42 PM, } 12, 46 -- Serialize complete at 04/28/2011 04:34:42 PM } 12, 46 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 20 -- From leunissen-at-aurion.nl Thu Apr 28 19:12:39 2011 9, 20 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3T0CZPF006465 9, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 19:12:38 -0500 9, 20 -- Received: from jan-desktop.lan ([219.89.62.96]) by fep01.xtra.co.nz 9, 20 -- with ESMTP 9, 20 -- id {20110429001224.ZVKY28201.fep01.xtra.co.nz-at-jan-desktop.lan} 9, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 29 Apr 2011 12:12:24 +1200 9, 20 -- Content-Type: text/plain; charset=us-ascii 9, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 9, 20 -- Subject: Re: [Microscopy] Re: HPF on invertebrate embryos 9, 20 -- From: Jan Leunissen {leunissen-at-aurion.nl} 9, 20 -- In-Reply-To: {201104282334.p3SNYpi6022426-at-ns.microscopy.com} 9, 20 -- Date: Fri, 29 Apr 2011 12:12:23 +1200 9, 20 -- Message-Id: {B3E1755B-CCBC-4C95-94B8-CA4C279302C4-at-aurion.nl} 9, 20 -- References: {201104282334.p3SNYpi6022426-at-ns.microscopy.com} 9, 20 -- To: Microscopy-at-microscopy.com 9, 20 -- X-Mailer: Apple Mail (2.1084) 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3T0CZPF006465 ==============================End of - Headers==============================
There's also those of us (who will go unnamed) who forget to remove the digital camera from the side port of the microscope when finished using the microscope. When we fire up the scope the next day at low mag and don't see the beam, it may take a while for the "light" to go on and remember that the camera's still blocking the beam!
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: Tracy.Lawrence-at-inspection.gc.ca [Tracy.Lawrence-at-inspection.gc.ca] Sent: Thursday, April 28, 2011 4:03 PM To: Haller, Edward
What an emotional day- tears to laughter! Thank you Jan.
Tracy
} } } Jan Leunissen - Aurion {leunissen-at-aurion.nl} 4/28/2011 12:03 pm } } } I should tell you what it was, so you can smile as well:
I was in Vienna last year to do some tests. And you should know that I am not actively involved with an institute, out of practice somewhat (in my defense, weak... but still). Had only worked with the Morgagni once or twice, trying to do all well along the instructions I had received. So ... switched on HT, started up the filament... nothing. Checked if I had made a mistake ... no, didn't seem like that, HT was on, we had a filament current as well as a beam current, but the TEM chamber was dark as.
I thought: I'll be b...d if I have to ask for help, after all those years being an electron microscopist.... And of course there is also that feeling of looking silly. After 10 minutes of trying, I gave up and was about to get help. Then I turned the room lights on... and there was the reason for not getting any beam: the cover was still on the glass pane of the viewing chamber! I sat there laughing for a few minutes and felt so silly. Decided to tell the friendly crew in Vienna anyway, but they still tease me with it, it was so funny.
Well, hope that made you smile too.
Cheers
Jan
On 29/04/2011, at 6:45 AM, Tracy.Lawrence-at-inspection.gc.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------- } } Thank you all for your suggestions. I hesitate to tell you what the } problem was but I guess its a learning experience...the shutter was } closed. } } Tracy } } } Hello All, } I am a relatively new user and only operator of our Hitachi-7100 TEM.
We have planned to buy the FESEM (Zeiss Supra 55) which will be served for all the units of the university (from dept of engineering to dept of medicine). However variable pressure (VP) mode caused indecision since we have critical point drier and sputter coater.
In the catalog of the FESEM, it is mentioned that variable pressure (VP) mode brings many advantages for imaging non-conducting specimens without charging artifacts.
Would you please inform me about the effectiveness of the VP mode (up to 133 Pa) at the FESEM (Zeiss Supra 55). Moreover, how does the VP mode affect the performance (negatively or positively) of the FESEM?
I tried to label end plates with rhodamine labelled bungarotoxin, but I didn't see any fluorescence after embedding in resin. I guess the dehydration killed it. I might try to label some NMJ specific receptor with a primary AB and then label the AB with a biotin or streptavidine secondary, expecting to see dark spots at the sites of the NMJs (end plates). But that is my "educated guess", I don't know if it actually works. Right now, I take the muscle sample (not from diaphragm) where the nerve branches to the smallest branches and try to section it there. Likewise, the probability to cut through a NMJ increases. So far, it goes well, last week in one day I hit NMJs from 4 different muscles. But before that I had a single sample which I was cutting for 2 consecutive days without success. When I re-checked it, it came out that the nerve was not branching enough there... After the Osmium treatment, the nerve is easy to follow and I can see it very nice in my samples (the muscle is very thin, I don't know if it will be the case with the diaphragm). binocular magnification of 3x was enough to localize the sites.
Hope this helps.
Regards,
Josif
P.S. Here come the auto-replies... One reason why I don't like posting to the list.
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS) Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y Dpto. Fisiologia Médica y Biofísica Universidad de Sevilla Facultad de Medicina Avda. Sánchez-Pizjuán 4 41009-Sevilla
-----Original Message----- X-from: Yankova-at-neuron.uchc.edu [mailto:Yankova-at-neuron.uchc.edu] Sent: Thursday, April 28, 2011 5:31 PM To: jmircheski-at-us.es
Hello everyone,
We have a researcher who wants to do TEM on motor end plates from a mouse diaphragm . Is there a good way to localize NMJ before we process the tissue for TEM? Any advice will be greatly appreciated.
Thanks, Maya Yankova, M.Sc. Research Assistant 3 Central Electron Microscopy Facility Department of Molecular, Microbial & Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3305 ph: 860.679.2395 yankova-at-neuron.uchc.edu
==============================Original Headers============================== 10, 25 -- From prvs=10926ea75f=yankova-at-neuron.uchc.edu Thu Apr 28 10:23:19 2011 10, 25 -- Received: from uchc.edu (itiron2.uchc.edu [155.37.201.27]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SFNJgv029971 10, 25 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 10:23:19 -0500 10, 25 -- Received: from ([10.91.15.93]) 10, 25 -- by itiron2.uchc.edu with ESMTP with TLS id CNJDJN1.5452318; 10, 25 -- Thu, 28 Apr 2011 11:23:16 -0400 10, 25 -- Received: from NSO-ITEXC-CCR04.uchc.net ([10.91.15.114]) by 10, 25 -- NSO-ITEXC-HT03.uchc.net ([::1]) with mapi; Thu, 28 Apr 2011 11:23:15 -0400 10, 25 -- From: "Yankova,Maya" {Yankova-at-neuron.uchc.edu} 10, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 25 -- Date: Thu, 28 Apr 2011 11:23:14 -0400 10, 25 -- Subject: TEM on motor end plates 10, 25 -- Thread-Topic: TEM on motor end plates 10, 25 -- Thread-Index: AQHMBbgxl7G/AbcWvUaVSsGJTymPIw== 10, 25 -- Message-ID: {8E7BE6D1E258C7449A99EAA21E6B9A5FAE8A248FFF-at-NSO-ITEXC-CCR04.uchc.net} 10, 25 -- Accept-Language: en-US 10, 25 -- Content-Language: en-US 10, 25 -- X-MS-Has-Attach: 10, 25 -- X-MS-TNEF-Correlator: 10, 25 -- acceptlanguage: en-US 10, 25 -- Content-Type: text/plain; charset="iso-8859-1" 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3SFNJgv029971 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 42 -- From jmircheski-at-us.es Fri Apr 29 10:03:33 2011 24, 42 -- Received: from mail.us.es (mail.us.es [193.147.175.20]) 24, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3TF3WaZ005276 24, 42 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2011 10:03:33 -0500 24, 42 -- Received: (qmail 8809 invoked from network); 29 Apr 2011 17:03:31 +0200 24, 42 -- Received: from unknown (HELO us.es) (192.168.2.12) 24, 42 -- by us.es with SMTP; 29 Apr 2011 17:03:31 +0200 24, 42 -- Received: (qmail 27383 invoked by uid 507); 29 Apr 2011 15:03:27 -0000 24, 42 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on antivirus2 24, 42 -- X-Spam-Level: * 24, 42 -- X-Spam-Status: No, score=1.6 required=7.5 tests=BAYES_50,DOS_OUTLOOK_TO_MX, 24, 42 -- FSL_HELO_NON_FQDN_1,HELO_NO_DOMAIN autolearn=disabled version=3.3.1 24, 42 -- Received: from 127.0.0.1 by antivirus2 (envelope-from {jmircheski-at-us.es} , uid 501) with qmail-scanner-2.08 24, 42 -- (clamdscan: 0.97/13024. 24, 42 -- Clear:RC:1(127.0.0.1):. 24, 42 -- Processed in 0.051624 secs); 29 Apr 2011 15:03:27 -0000 24, 42 -- Received: from unknown (HELO antivirus2) (127.0.0.1) 24, 42 -- by us.es with SMTP; 29 Apr 2011 15:03:27 -0000 24, 42 -- Received: from 192.168.1.13 (192.168.1.13) 24, 42 -- by antivirus2 (F-Secure/fsigk_smtp/403/antivirus2); 24, 42 -- Fri, 29 Apr 2011 17:03:27 +0200 (CEST) 24, 42 -- X-Virus-Status: clean(F-Secure/fsigk_smtp/403/antivirus2) 24, 42 -- Received: (qmail 24478 invoked from network); 29 Apr 2011 17:03:27 +0200 24, 42 -- Received: from macarfis.us.es (HELO cubiculos) (jmircheski-at-193.147.167.92) 24, 42 -- by us.es with RC4-MD5 encrypted SMTP; 29 Apr 2011 17:03:27 +0200 24, 42 -- Return-Receipt-To: "Josif Mircheski" {jmircheski-at-us.es} 24, 42 -- From: "Josif Mircheski" {jmircheski-at-us.es} 24, 42 -- To: {Yankova-at-neuron.uchc.edu} 24, 42 -- Cc: {microscopy-at-microscopy.com} 24, 42 -- References: {201104281530.p3SFUgC5011480-at-ns.microscopy.com} 24, 42 -- In-Reply-To: {201104281530.p3SFUgC5011480-at-ns.microscopy.com} 24, 42 -- Subject: RE: [Microscopy] TEM on motor end plates 24, 42 -- Date: Fri, 29 Apr 2011 17:03:26 +0200 24, 42 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAEPQoVbwH5VIu5W5KqvqHQfCgAAAEAAAAEjg4BQuwqpJjamQ3uympW8BAAAAAA==-at-us.es} 24, 42 -- MIME-Version: 1.0 24, 42 -- Content-Type: text/plain; 24, 42 -- charset="iso-8859-1" 24, 42 -- X-Mailer: Microsoft Office Outlook 12.0 24, 42 -- Thread-index: AcwFuUC7aFlz0wuTSTWHw1OrffZQ2wAwxfSA 24, 42 -- Content-Language: en-us 24, 42 -- Content-Transfer-Encoding: 8bit 24, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3TF3WaZ005276 ==============================End of - Headers==============================
The resolution should not be greatly affected by VP, but secondary electrons are weak and are easily deflected by bigger things in the environment like heavy gas molecules. The upside is that VP (gas assisted SEM) depresses charging and for many specimens, that will be critical. There is little to lose by having the VP capability on new SEMs - unless users of the VP mode are permitted to view/study specimens with substantial volatiles which leave contaminants behind. These latter specimens should only be studied in conjunction with the use of a cryo accessory. On our ESEM, we can add a 'cold' stage to help restrain volatiles (like HOH in hydrated specimens - we can work up to 20 Torr). VP can ameliorate the effect of having a poorly regulated HV supply that can't maintain constant power delivery over long periods of time at low accelerating voltages - thus, for really good EDS, carefully spec your voltage regulation expectations. We have a 6kVA UPS, and even it doesn't overcome the weakness of poor regulation in the power supply of the scope. As low voltage is the only other non-coating method of depressing charging, the absence of VP can leave one with poor imaging for Point-and-ID or Mapping with EDS.
The biggest effect of gas in the chamber is alleged to be on X-Ray analysis. [see Home Page below, Links, and articles and other info on the subject - especially those from NIST. OR by this direct URL: http://cmirt.wcupa.edu/CMIRT_links.html#ESEM ]
I also recommend the following book: http://onlinelibrary.wiley.com/doi/10.1002/9780470758731.fmatter/pdf. It is about $65 on Amazon and will help all potential users to estimate the utility of VP as well as get a dose of its theoretical foundations (~220 pages-hardbound).
We survey cut slabs of rock up to 95 x 95 x 10mm, and almost all geologic thin sections (100um) of rock at 70 Pa in our Fei Quanta 400 ESEM with Oxford INCA EDS + Feature/GSR and Automate. Typical results can be seen on our site:
http://cmirt.wcupa.edu/P-07-70-22/P-07-70-22-Montage-Map-Garnet-1.htm [NOTE: scale bar on montage]
Good luck, and hope this helps!
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu
Original Message----- X-from: albayrakonder-at-gmail.com [mailto:albayrakonder-at-gmail.com] Sent: Friday, April 29, 2011 10:33 AM To: Monson, Frederick
Dear All,
We have planned to buy the FESEM (Zeiss Supra 55) which will be served for all the units of the university (from dept of engineering to dept of medicine). However variable pressure (VP) mode caused indecision since we have critical point drier and sputter coater.
In the catalog of the FESEM, it is mentioned that variable pressure (VP) mode brings many advantages for imaging non-conducting specimens without charging artifacts.
Would you please inform me about the effectiveness of the VP mode (up to 133 Pa) at the FESEM (Zeiss Supra 55). Moreover, how does the VP mode affect the performance (negatively or positively) of the FESEM?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both crnl_srbu-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Natl. Inst. for Materials Physics, Bucharest, Romania
Title-Subject: [Filtered] Reply to "TEM of magnetic particles"
Message: In order to avoid any problem with deposition of nanoparticles on OL pole piece you can adopt the extreme solution of embedding the particles in a resin and afterwards processing the sample for TEM as if it were a solid sample. It is time consuming, but is the safest. I use that procedure when I do TEM on small grains of magnetic alloys. It takes time and effort to prepare the sample, but it guarantees no deposition of particles on OL pole piece will occur. Corneliu Sarbu Natl. Inst. for Materials Physics Bucharest-Magurele Romania Tuesday, April 26, 2011 8:03 PM } From: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} Add sender to Contacts To: crnl_srbu-at-yahoo.com
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
When I was at Western Digital, the SEMs would periodically need to have the final pole piece cleaned of magnetic particles, and these samples were solid sputtered films with obvious no loose particles and sub 10nm grain size, not powders. Be very careful with loose particles.
A. John Mardinly, ASU
-----Original Message----- X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] Sent: Tuesday, April 26, 2011 7:51 AM To: John Mardinly
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both brian.mcintyre-at-rochester.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: brian.mcintyre-at-rochester.edu Name: Brian McIntyre
Organization: Univ of Rochester
Title-Subject: [Filtered] Student projects
Message: Hi Listers-
If you have time please checkout the web versions of this years projects for the Univ of Rochester's Electron Microscopy course. Some interesting stuff...
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yankova-at-neuron.uchc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Electron microscopy on motor end plates
Message: Hello everyone,
We have a researcher who wants to do EM on a motor endplate of a mouse diaphragm. We would like to find a protocol to localize the motor endplates on the muscle before the tissue is processed for EM. Any advice would be greatly appreciated.
Thanks
Maya Yankova, M.Sc. Research Assistant 3 Central Electron Microscopy Facility Department of Molecular, Microbial & Structural Biology University of Connecticut Health Center 263 Farmington Avenue Farmington, CT 06030-3305 ph: 860.679.2395 yankova-at-neuron.uchc.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Tracy.Lawrence-at-inspection.gc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Tracy.Lawrence-at-inspection.gc.ca Name: Tracy Lawrence
Organization: CFIA
Title-Subject: [Filtered] Alignment of TEM
Message: I am a relatively new user of our Hitachi H-7100. I have very little experience in aligning the TEM and therefore, troubleshooting the alignment is very challenging. After a month of not using the microscope (it was left in Evac On) I turned the insturment on and got only a diffuse beam of light. Focusing the beam made it only slightly brighter but did not focus the beam. I backed out both the condenser and objective apertures to check gun alignment and I manipulated the gun horizontal and tilt until I got the brightest light but still unable to focus the beam. If I move to Spot Size 7 I can see a very small sliver of bright light off to the left of the phosphorus screen. I am not sure if this is my beam and I am unable to move it to the phosphorus screen. I am at a loss as to what to do. This problem is well below the level of issues discussed on this Listserv so if anyone can offer some guidance on alignment strategies they are welcome to contact me personally.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Organization: LBNL
Title-Subject: [Filtered] SiN TEM Windows
Message: Hello All,
Does anyone have an idea about how to separate the SiN film from the TEM window frame? I want to preserve the structural integrity of the film to use as a substrate for particle analysis. Thanks!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both petereaton-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: petereaton-at-hotmail.com Name: Peter Eaton
Organization: University of Porto, POrtugal
Title-Subject: [Filtered] Artifacts in aldehyde fixed cells
Message: Hi, I remember seeing some discussion (many years ago?), on the server about artifacts in microscopy of biological specimens in general. Specifically I think I read about membrane blebbing caused by aldehyde-based fixing. Does anyone have any actual references about this? I have seen what might be this effect, but it's hard to be sure. Thanks, Pete.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both benj-at-duke.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: benj-at-duke.edu Name: Ben
Organization: Duke university / CEE dpt.
Title-Subject: [Filtered] SEM of particles suspended in oil.
Message: Hello, I was wondering if someone had a method to visualize, with SEM, fine particles suspended in an oil phase. Let me know Thanks much Best Ben Login Host: 152.3.68.8 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 30 14:41:55 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3UJft52001833 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 5105938DB71 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id YXEy7EfcwYYc for {microscopy-at-microscopy.com} ; 7, 25 -- Sat, 30 Apr 2011 14:41:54 -0500 (CDT) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id CEED338DB66 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:54 -0500 (CDT) 7, 25 -- Message-ID: {4DBC6602.4090102-at-microscopy.com} 7, 25 -- Date: Sat, 30 Apr 2011 14:41:54 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: benj-at-duke.edu 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:SEM of particles suspended in oil 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cvierret-at-mst.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: Missouri Universityof Science and Technology
Title-Subject: [Filtered] Chamberscopes
Message: Greetings,
Does anyone have experience with a SEMtech chamber scope? If so in specific does anyone have experience with a SEMtech chamberscope on a Hitachi S4700? Please respond in private.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckee-at-helix.mgh.harvard.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mckee-at-helix.mgh.harvard.edu Name: Mary McKee
Organization: MGH
Title-Subject: [Filtered] does paraformaldehye fixation of human tissue inactivate potentially infective viruses like HIV?
Message: We are going to be cutting paraformaldehyde-fixed human tissue on our cryostats. My understanding was that paraformaldehyde does not inactivate HIV. Does anyone know if this is true? Thanks in advance.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both albayrakonder-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
We have planned to buy the FESEM which will be served for all the units of the university (from dept of engineering to dept of medicine). However variable pressure (VP) mode caused indecision. (we have critical point drier and sputter coater).
In the catalog of the FESEM, it is mentioned that variable pressure (VP) mode brings many advantages for imaging non-conducting specimens without charging artifacts. Would you please inform me about the effectiveness of the VP mode (up to 133 Pa) at the FESEM (Zeiss Supra 55). Moreover, how does the VP mode affect the performance (negatively or positively) of the FESEM?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jds451-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jds451-at-psu.edu Name: Jen Sloppy
Organization: Drexel University
Title-Subject: [Filtered] Bakelite in UHV ?
Message: Hi all, I have some samples mounted in Bakelite that I plan to study with XPS. Does anybody know if Bakelite outgasses? Is it mandatory to liberate the specimens from their mounts before loading them into the chamber?
As far as I recall, Bakelite is a trade name for plastics with phenolic resin, and if so then it would outgass like mad. I'd keep Bakelite out of vacuum system, if at all possible...
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/1/2011 5:54 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both jds451-at-psu.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: jds451-at-psu.edu Name: Jen Sloppy } } Organization: Drexel University } } Title-Subject: [Filtered] Bakelite in UHV ? } } Message: Hi all, } I have some samples mounted in Bakelite that I plan to study with XPS. } Does anybody know if Bakelite outgasses? Is it mandatory to liberate the } specimens from their mounts before loading them into the chamber? } } Thanks in advance for any advice. } } - Jen Sloppy } } Login Host: 68.80.76.57 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun May 1 16:53:32 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p41LrWG4003101 } 10, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2011 16:53:32 -0500 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 6420138E838 } 10, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2011 16:53:32 -0500 (CDT) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id tCC4We9NFZnK for {microscopy-at-microscopy.com} ; } 10, 25 -- Sun, 1 May 2011 16:53:29 -0500 (CDT) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id D487C38E828 } 10, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2011 16:53:29 -0500 (CDT) } 10, 25 -- Message-ID: {4DBDD659.8050208-at-microscopy.com} } 10, 25 -- Date: Sun, 01 May 2011 16:53:29 -0500 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: jds451-at-psu.edu } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: viaWWW:Bakelite in UHV ? } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 3, 33 -- From vray-at-partbeamsystech.com Sun May 1 18:10:47 2011 3, 33 -- Received: from nm4.bullet.mail.sp2.yahoo.com (nm4.bullet.mail.sp2.yahoo.com [98.139.91.74]) 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p41NAkpH019751 3, 33 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2011 18:10:46 -0500 3, 33 -- Received: from [98.139.91.67] by nm4.bullet.mail.sp2.yahoo.com with NNFMP; 01 May 2011 23:10:46 -0000 3, 33 -- Received: from [98.139.91.14] by tm7.bullet.mail.sp2.yahoo.com with NNFMP; 01 May 2011 23:10:46 -0000 3, 33 -- Received: from [127.0.0.1] by omp1014.mail.sp2.yahoo.com with NNFMP; 01 May 2011 23:10:46 -0000 3, 33 -- X-Yahoo-Newman-Id: 275830.56515.bm-at-omp1014.mail.sp2.yahoo.com 3, 33 -- Received: (qmail 39188 invoked from network); 1 May 2011 23:10:45 -0000 3, 33 -- Received: from [192.168.2.31] (vray-at-75.68.231.25 with plain) 3, 33 -- by smtp111.biz.mail.mud.yahoo.com with SMTP; 01 May 2011 16:10:45 -0700 PDT 3, 33 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 3, 33 -- X-YMail-OSG: FzPi.HMVM1nKzCVmccAVfS8A55N4f0TXYnzP.XOinSbSvLR 3, 33 -- Ug24taGeuQ4YecieCxYc76bCg81gb7kGxxYxSBUZLoeezDgujZFETD0OMEVQ 3, 33 -- lK7LQAjGD3SMPyN6d_737druqTzO_d1PVF.Pj0xTlIy78rTvUyNTa0tPcGrH 3, 33 -- dVZ6ODVEx3X8w7tODCq3IkiyX0_CXmAdgmOLg7RhRvqHM.7q4g39jwcMSUD3 3, 33 -- OU3.AebfcYN08.EOq0nrMC09.Z9Ovx7S_j7zr69V.1DxHEpQeYEDhku.0auO 3, 33 -- MGfT7KhqTqzLn6dbC_GRaQXr.LPMTBYy38NQEm20b1AjLMA3RhSHVQarW82a 3, 33 -- _ybrLOdzA.boJHuCYtQ_VOr.8alFCdJxA3RjEkgXXO0iJTw-- 3, 33 -- X-Yahoo-Newman-Property: ymail-3 3, 33 -- Message-ID: {4DBDE877.8020905-at-partbeamsystech.com} 3, 33 -- Date: Sun, 01 May 2011 19:10:47 -0400 3, 33 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 3, 33 -- Reply-To: vray-at-partbeamsystech.com 3, 33 -- Organization: PBS&T 3, 33 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 3, 33 -- MIME-Version: 1.0 3, 33 -- To: microscopy-at-microscopy.com, jds451-at-psu.edu 3, 33 -- Subject: Re: [Microscopy] viaWWW:Bakelite in UHV ? 3, 33 -- References: {201105012154.p41Lsd8n004442-at-ns.microscopy.com} 3, 33 -- In-Reply-To: {201105012154.p41Lsd8n004442-at-ns.microscopy.com} 3, 33 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 33 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Bakelite is a phenol-formaldehyde polymer. If properly compounded it should be fully cross-linked, and therefore should have a very low vapor pressure.
The problem with metal and ceramic specimens mounted in Bakelite, however, is that they have usually undergone a series of polishing and etching treatments. Often there are tiny cracks in the specimens themselves (especially if they are ceramics), or between the specimen and the surrounding Bakelite. Then small amounts of the liquids used in the polishing and etching processes can get into these cracks and remain there for a long time. If this happens these fluids can evolve when they are put into a vacuum system, and thereby degrade the vacuum.
It is therefore difficult to predict whether or not Specimens mounted in Bakelite (or any other material for that matter) will give you problems. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 15 -- From bigelow-at-umich.edu Sun May 1 19:05:36 2011 3, 15 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.12.86]) 3, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4205acW003849 3, 15 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2011 19:05:36 -0500 3, 15 -- Received: FROM [76.226.123.150] (adsl-76-226-123-150.dsl.sfldmi.sbcglobal.net [76.226.123.150]) 3, 15 -- By tombraider.mr.itd.umich.edu ID 4DBDF54E.C418E.27141 ; 3, 15 -- Authuser bigelow; 3, 15 -- 1 May 2011 20:05:35 EDT 3, 15 -- Mime-Version: 1.0 3, 15 -- Message-Id: {p06240803c9e3a380e698-at-[76.226.123.150]} 3, 15 -- Date: Sun, 1 May 2011 20:05:24 -0400 3, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 15 -- Subject: [Microscopy]Bakelite 3, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
You raise a fundamental question for biology microsopists and here I would like to remind you of another, a bit neglected, player in the process of aldehyde cell fixation. This player is TIME: How much time it takes for the fixative to diffuse and reach a cell within a piece of tissue. And more importantly, how much time passes from the moment the first tiny toxic concentration of the fixative reaches the cell until it rises to a lethal concentration and fixes this cell. And even before that moment, for how much time this cell was exposed to alarm signals emitted by neighbor cells in response to their contact with the toxic fixative milieu, and transmitted to this cell through cell to cell contacts and junctions.
If I was a cell I would be in a state of panic during these last moments of my life, feeling the catastrophy caused by the aldehydes that destroy my enviroment and my family and is going to kill me too. The countless cell functions occurring in live cells may change at sub-seconds rate. For instance flagellae and ciliae beat three times per second, pino- and exocytosis of plasma membrane is fast, and large molecules may change within microseconds. Membrane blebbing can happen very rapidly, perhaps in the course of activation of apoptotic mechanisms during this deadly fight of the cell with the aldehydes. I don't know well the physiochemical properties of aldehydes to estimate their diffusion times in the various animal or plant tissues, but I am sure that they don't act instantly.
X-from the diffusion point of view, epithelial and other mesothelial or endothelial cells lining tissue cavities would get fixed faster and better, because a large percentage of their surface is immediately exposed to the fixative. Ideally cells in suspension can be fixed instantly and then they can be observed under a microscope to provide a snapshot of live cells. Even so, what we see is far away from life. For instance, if you observe motile spermatozoa under light microscopy they look like snakes, but if you fix them they look more like spaghetti. And if this is true for cells like sperm that are prepared to come out of the body, what about a tissue we excise from an animal or during surgery and then we fix it. The cells in this tissue, deprived from their environment and blood supply, change rapidly and every single moment passing until final fixation counts.
I think that all these parameters together with the entire post-fixation preparation of the sample are of paramount importance when we make our microscopic observations and interpret our findings. In this sense the word "artifact" sounds a little bit like aphorism. We judge what images are closer to the "real" and ideal state we have in our mind (aesthetics are always involved) and we choose them to represent our findings. The rest of the images we reject, and we like to condemn them as artifacts or artefacts. In a way, we always choose something that we wish to see and we have to be very careful at this point. Pythagoras the philosopher forbade to his pupils to make wishes, on the grounds that they are too young and don't really know what to wish.
My apologies for much philosophy -we Greeks are good in this- and not providing references. I would love to hear yours and other colleagues' views on this subject.
Email: petereaton-at-hotmail.com Name: Peter Eaton
Organization: University of Porto, POrtugal
Title-Subject: [Filtered] Artifacts in aldehyde fixed cells
Message: Hi, I remember seeing some discussion (many years ago?), on the server about artifacts in microscopy of biological specimens in general. Specifically I think I read about membrane blebbing caused by aldehyde-based fixing. Does anyone have any actual references about this? I have seen what might be this effect, but it's hard to be sure. Thanks, Pete.
==============================Original Headers============================== 24, 25 -- From eikonika-at-otenet.gr Mon May 2 00:26:04 2011 24, 25 -- Received: from chimaera.otenet.gr (chimaera.otenet.gr [83.235.69.16]) 24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p425Q3bX024309 24, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 00:26:04 -0500 24, 25 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr [83.235.67.30]) 24, 25 -- by chimaera.otenet.gr (ESMTP) with ESMTP 24, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 08:26:02 +0300 (EEST) 24, 25 -- Received: from pegasus (athedsl-228738.home.otenet.gr [85.74.213.32]) 24, 25 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id p425PwYk012214 24, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 08:26:02 +0300 24, 25 -- Message-ID: {0C8A89B40DA94F34B3E1460CF3F19DAB-at-pegasus} 24, 25 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 24, 25 -- To: {microscopy-at-microscopy.com} 24, 25 -- Subject: Artifacts in aldehyde fixed cells 24, 25 -- Date: Mon, 2 May 2011 08:25:51 +0300 24, 25 -- MIME-Version: 1.0 24, 25 -- Content-Type: text/plain; 24, 25 -- format=flowed; 24, 25 -- charset="iso-8859-7"; 24, 25 -- reply-type=original 24, 25 -- Content-Transfer-Encoding: 7bit 24, 25 -- X-Priority: 3 24, 25 -- X-MSMail-Priority: Normal 24, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5843 24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
Will Bigelow made some comments that are right on the money. It has to be fully cross linked and can't have the polishing compounds in the cracks. You have a marvelous tool in the SEM to determine if you are going to see a contamination layer in the UHV XPS system. Although the SEM doesn't have quite as good of a vacuum as the XPS, it is still very good and the beam is very sensitive to hydrocarbon contamination. If you don't see contamination in the SEM, then your sample should be good in the XPS.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
US Toll Free: 1-800-728-2233 Tel: (949) 492-2600 Fax: (949) 492-1499
www.southbaytech.com SWalck-at-SouthBayTech.com
On Sun 01/05/11 3:03 PM , microscopylistserver-noreply-at-microscopy.com sent: } } } } --------------------------------------------------------------------------- } -The Microscopy ListServer -- CoSponsor: The Microscopy Society of } AmericaTo Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserverOn-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html--------------------------------------------------------------------------- } - } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html--------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both jds451-at-psu.edu } as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: jds451-at-psu.edu } Name: Jen Sloppy } Organization: Drexel University } } Title-Subject: [Filtered] Bakelite in UHV ? } } Message: Hi all, } I have some samples mounted in Bakelite that I plan to study with XPS. } Does anybody know if Bakelite outgasses? Is it mandatory to liberate the } specimens from their mounts before loading them into the chamber? } } Thanks in advance for any advice. } } - Jen Sloppy } } Login Host: 68.80.76.57 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers==============================10, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun May 1 } 16:53:32 201110, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p41LrWG400310110, 25 -- for {mic } roscopy-at-microscopy.com} ; Sun, 1 May 2011 16:53:32 -050010, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 6420138E838 } 10, 25 -- for {mic } roscopy-at-microscopy.com} ; Sun, 1 May 2011 16:53:32 -0500 (CDT)10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, } port 10024)10, 25 -- with ESMTP id tCC4We9NFZnK for {mic } roscopy-at-microscopy.com} ;10, 25 -- Sun, 1 May 2011 16:53:29 -0500 (CDT) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22])10, 25 -- by znl.com (Postfix) with ESMTPA id D487C38E828 } 10, 25 -- for {mic } roscopy-at-microscopy.com} ; Sun, 1 May 2011 16:53:29 -0500 (CDT)10, 25 -- Message-ID: {4DBDD659.8050208-at-microscopy.com} 10, 25 -- Date: Sun, 01 May 2011 16:53:29 -0500 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: jds451-at-psu.edu } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.410, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {mic } roscopy-at-microscopy.com} 10, 25 -- Subject: viaWWW:Bakelite in UHV ? } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 10, 19 -- From swalck-at-southbaytech.com Mon May 2 00:46:11 2011 10, 19 -- Received: from www5.webmail.pair.com (www5.webmail.pair.com [66.39.3.83]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p425kBuT008031 10, 19 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 00:46:11 -0500 10, 19 -- Received: (qmail 85962 invoked from network); 2 May 2011 05:46:11 -0000 10, 19 -- Received: from localhost.pair.com (HELO localhost) (127.0.0.1) 10, 19 -- by localhost.pair.com with SMTP; 2 May 2011 05:46:11 -0000 10, 19 -- MIME-Version: 1.0 10, 19 -- X-Mailer: AtMail PHP 5.5 10, 19 -- Message-ID: {50248.1304315171-at-southbaytech.com} 10, 19 -- To: {microscopy-at-microscopy.com} 10, 19 -- Reply-To: swalck-at-southbaytech.com 10, 19 -- Content-Type: text/plain; charset="utf-8" 10, 19 -- X-Origin: 72.197.11.236 10, 19 -- Date: Mon, 02 May 2011 01:46:11 -0400 10, 19 -- Subject: Re: [Microscopy] viaWWW:Bakelite in UHV ? 10, 19 -- From: Scott Walck {swalck-at-southbaytech.com} 10, 19 -- Content-Transfer-Encoding: 8bit 10, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p425kBuT008031 ==============================End of - Headers==============================
The only paper I'm readily familiar with (I'm sure there are others), is this one:
G.J. Hyde, S. Lancelle, P.K. Hepler, A.R. Hardham (1991) Freeze substitution reveals a new model for sporangial cleavage in Phytophthora, a result with implications for cytokinesis in other eukaryotes. Journal of Cell Science 100, 735-746 http://jcs.biologists.org/content/100/4/735.abstract
There are a number of citing articles on a similar theme.
cheers, Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 1/05/11 5:52 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both petereaton-at-hotmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: petereaton-at-hotmail.com Name: Peter Eaton } } Organization: University of Porto, POrtugal } } Title-Subject: [Filtered] Artifacts in aldehyde fixed cells } } Message: Hi, } I remember seeing some discussion (many years ago?), on the server about } artifacts in microscopy of biological specimens in general. Specifically } I think I read about membrane blebbing caused by aldehyde-based fixing. } Does anyone have any actual references about this? I have seen what } might be this effect, but it's hard to be sure. Thanks, } Pete. } } Login Host: 193.136.24.81 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 30 14:41:28 } 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p3UJfSeA032450 } 8, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:28 -0500 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id E0D7238DB65 } 8, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:28 -0500 } (CDT) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } 10024) } 8, 25 -- with ESMTP id 8+x2YDhuvD5K for {microscopy-at-microscopy.com} ; } 8, 25 -- Sat, 30 Apr 2011 14:41:28 -0500 (CDT) } 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id A3EC238DB5A } 8, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:28 -0500 } (CDT) } 8, 25 -- Message-ID: {4DBC65E8.5060703-at-microscopy.com} } 8, 25 -- Date: Sat, 30 Apr 2011 14:41:28 -0500 } 8, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: petereaton-at-hotmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:Artifacts in aldehyde fixed cells } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 32 -- From prvs=096e80240=Rosemary.White-at-csiro.au Mon May 2 01:33:38 2011 12, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) 12, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p426XbDl024713 12, 32 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 01:33:37 -0500 12, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 12, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 12, 32 -- s=email; t=1304318018; x=1335854018; 12, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 12, 32 -- content-transfer-encoding; 12, 32 -- bh=M9KWMfHCx1jJOzIjGTrgFcvBwDkDa04FFiMi7fB/yf4=; 12, 32 -- b=N4BIRSxbH7p/TXC81i82iaX2oGN6G30WjU8xtdb2hZydrFeA/e4i4p/F 12, 32 -- B8mEd/uo1mGJaISRkcaOyNu2QgFGka5P5pUcxP3B6NY1etKe7KNJm+UY7 12, 32 -- oIsfWOS3nB9Jd3y; 12, 32 -- X-IronPort-AV: E=Sophos;i="4.64,300,1301839200"; 12, 32 -- d="scan'208";a="19915750" 12, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 12, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 02 May 2011 16:33:36 +1000 12, 32 -- Received: from [152.83.195.117] (152.83.195.117) by 12, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 12, 32 -- (TLS) id 8.3.137.0; Mon, 2 May 2011 16:33:35 +1000 12, 32 -- User-Agent: Microsoft-Entourage/12.28.0.101117 12, 32 -- Date: Mon, 2 May 2011 16:33:33 +1000 12, 32 -- Subject: Re: [Microscopy] viaWWW:Artifacts in aldehyde fixed cells 12, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} 12, 32 -- To: {microscopy-at-microscopy.com} 12, 32 -- Message-ID: {C9E48D5D.236DE%rosemary.white-at-csiro.au} 12, 32 -- Thread-Topic: [Microscopy] viaWWW:Artifacts in aldehyde fixed cells 12, 32 -- Thread-Index: AcwHcC/FpXffnZ4uQYCjwSEf8RVWVQBIqvf/ 12, 32 -- In-Reply-To: {201104301952.p3UJqk1l010906-at-ns.microscopy.com} 12, 32 -- MIME-Version: 1.0 12, 32 -- Content-Type: text/plain; charset="US-ASCII" 12, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Yes sir I did them all: forget the side camera, the gun valve and the screen cover. I confess I did them all! May I go in peace, now?
Think positive: the worse in the mistake, the better you remember it. I've never forgotten the screen cover twice!
regards,
Stephane
----- Original Message ---- X-from: "ehaller-at-health.usf.edu" {ehaller-at-health.usf.edu} To: nizets2-at-yahoo.com Sent: Fri, April 29, 2011 2:23:29 PM
There's also those of us (who will go unnamed) who forget to remove the digital camera from the side port of the microscope when finished using the microscope. When we fire up the scope the next day at low mag and don't see the beam, it may take a while for the "light" to go on and remember that the camera's still blocking the beam!
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: Tracy.Lawrence-at-inspection.gc.ca [Tracy.Lawrence-at-inspection.gc.ca] Sent: Thursday, April 28, 2011 4:03 PM To: Haller, Edward
What an emotional day- tears to laughter! Thank you Jan.
Tracy
} } } Jan Leunissen - Aurion {leunissen-at-aurion.nl} 4/28/2011 12:03 pm } } } I should tell you what it was, so you can smile as well:
I was in Vienna last year to do some tests. And you should know that I am not actively involved with an institute, out of practice somewhat (in my defense, weak... but still). Had only worked with the Morgagni once or twice, trying to do all well along the instructions I had received. So ... switched on HT, started up the filament... nothing. Checked if I had made a mistake ... no, didn't seem like that, HT was on, we had a filament current as well as a beam current, but the TEM chamber was dark as.
I thought: I'll be b...d if I have to ask for help, after all those years being an electron microscopist.... And of course there is also that feeling of looking silly. After 10 minutes of trying, I gave up and was about to get help. Then I turned the room lights on... and there was the reason for not getting any beam: the cover was still on the glass pane of the viewing chamber! I sat there laughing for a few minutes and felt so silly. Decided to tell the friendly crew in Vienna anyway, but they still tease me with it, it was so funny.
Well, hope that made you smile too.
Cheers
Jan
On 29/04/2011, at 6:45 AM, Tracy.Lawrence-at-inspection.gc.ca wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ---------------------------------------------------------------------------- } } Thank you all for your suggestions. I hesitate to tell you what the } problem was but I guess its a learning experience...the shutter was } closed. } } Tracy } } } Hello All, } I am a relatively new user and only operator of our Hitachi-7100 TEM.
} I went to use the instrument for the first time in a month and was not } able to find the beam. I have bright illumination but no focussed beam. } I have backed out the apertures and specimen holder to adjust the Gun } alignment to get the brightest illumination at the largest spot size. } There looks to be somewhat of a beam at the top of the screen but I am } unable to get it to the centre without losing it all together. } } I know this problem is well below the level of discussion on this } ListServ so if you have any suggestions please feel free to contact me } off line. } } Thank you Tracy } } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } Tracy Lawrence } (250) 363-6650 ext 304 | Tracy.Lawrence-at-inspection.gc.ca } Facsimile / Télécopieur :(250)363-6661 } Virology Technician, Canadian Food Inspection Agency } Agence Canadienne d'inspection des aliments } 8801 East Saanich Road | 8801 Rue Saanich Est Sidney BC V8L 1H3 } Government of Canada | Gouvernement du Canada } www.inspection.gc.ca } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ } } } } ==============================Original Headers============================== } 9, 16 -- From Tracy.Lawrence-at-inspection.gc.ca Thu Apr 28 13:45:03 2011 } 9, 16 -- Received: from agrpazsmtp8.agr.gc.ca (agrpazsmtp8.agr.gc.ca [192.197.71.119]) } 9, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3SIj2jY001441 } 9, 16 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Apr 2011 13:45:03 -0500 } 9, 16 -- Message-Id: {4DB9533B.2FFA.006A.1-at-inspection.gc.ca} } 9, 16 -- X-Mailer: Novell GroupWise Internet Agent 7.0.3 } 9, 16 -- Date: Thu, 28 Apr 2011 14:45:00 -0400 } 9, 16 -- From: "Tracy Lawrence" {Tracy.Lawrence-at-inspection.gc.ca} } 9, 16 -- To: {Microscopy-at-microscopy.com} } 9, 16 -- Subject: TEM Alignment } 9, 16 -- Mime-Version: 1.0 } 9, 16 -- Content-Type: text/plain; charset=UTF-8 } 9, 16 -- Content-Dispositi on: inline } 9, 16 -- Received-SPF: none } 9, 16 -- Content-Transfer-Encoding: 8bit } 9, 16 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p3SIj2jY001441 } ==============================End of - Headers==============================
This is a principle of science that any interaction with the specimen modifies its nature, however we all strive to limit the modifications we introduce. The formaldehyde/glutaraldehyde mix is used for decades now and frankly I won't dare stand up against the huge amount of literature produced with the classical methods and claim that they introduce systematically significant artifacts. What you can say is that you lose some structures, that you don't see everything. This is right (and this is confirmed by the use of cryofixation). The facts are that formaldehyde is a fast-penetrating fixative, but its fixation is weak and (at least partly) reversible. This is why it is often used in association with glutaraldehyde, which is slower but stronger. Both together generally offer a fixation of quality. However I have a problem with the original message of Pete and specifically with the part "artifacts...in general". There is no protocol which works "in general". All protocols must be adapted to the specimen.
In general, with protocols well adapted for the specimen, formaldehyde fixation does not produce membrane blebbing artifacts. However it may well be that a general protocol is not suited for Pete's specimen. Also, generally formaldehyde fixation is completed by an osmium post-fixation step but this was not stated. Not knowing with which specimen Pete is working (and how) it is hard to guess what could be wrong, but I wouldn't be surprised if it had to do with osmoticity.
Have a nice week all. Summer just ended in Austria ;-)
Stephane
----- Original Message ---- X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Sat, April 30, 2011 9:48:52 PM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both petereaton-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: petereaton-at-hotmail.com Name: Peter Eaton
Organization: University of Porto, POrtugal
Title-Subject: [Filtered] Artifacts in aldehyde fixed cells
Message: Hi, I remember seeing some discussion (many years ago?), on the server about artifacts in microscopy of biological specimens in general. Specifically I think I read about membrane blebbing caused by aldehyde-based fixing. Does anyone have any actual references about this? I have seen what might be this effect, but it's hard to be sure. Thanks, Pete.
An early paper showing membrane induced artifacts resulting from aldehyde fixation: Hasty & Hay (1978) Freeze-fracture studies of the developing cell surface. II. Particle-Free Membrane Blisters on Glutaraldehyde-Fixed Corneal Fibroblasts Are Artefacts. J. Cell Biology 78:756-768.
There are other papers showing the dramatic change in morphology to mitochondria following aldehyde fixation compared to those prepared by high pressure freezing or quick-freezing using the metal mirror approach. I don't have a copy to double check but I believe that is shown in Terracio, L, Bankston, PW and McAteer, JA (1981) Ultrastructural observations on tissues processed by a quick-freezing, rapid-drying method: Comparison with conventional specimen preparation. Cryobiology 18(1):55-71
Here is a study showing aldehydes cause dramatic changes in endosomes: Murk JL, Posthuma G, Koster AJ, Geuze HJ, Verkleij AJ, Kleijmeer MJ, Humbel BM. (2003) Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography. J Microsc. 212(Pt 1):81-90.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: eikonika-at-otenet.gr [mailto:eikonika-at-otenet.gr] Sent: Monday, May 02, 2011 12:27 AM To: Phillips, Thomas E.
Dear Pete
You raise a fundamental question for biology microsopists and here I would like to remind you of another, a bit neglected, player in the process of aldehyde cell fixation. This player is TIME: How much time it takes for the fixative to diffuse and reach a cell within a piece of tissue. And more importantly, how much time passes from the moment the first tiny toxic concentration of the fixative reaches the cell until it rises to a lethal concentration and fixes this cell. And even before that moment, for how much time this cell was exposed to alarm signals emitted by neighbor cells in response to their contact with the toxic fixative milieu, and transmitted to this cell through cell to cell contacts and junctions.
If I was a cell I would be in a state of panic during these last moments of my life, feeling the catastrophy caused by the aldehydes that destroy my enviroment and my family and is going to kill me too. The countless cell functions occurring in live cells may change at sub-seconds rate. For instance flagellae and ciliae beat three times per second, pino- and exocytosis of plasma membrane is fast, and large molecules may change within microseconds. Membrane blebbing can happen very rapidly, perhaps in the course of activation of apoptotic mechanisms during this deadly fight of the cell with the aldehydes. I don't know well the physiochemical properties of aldehydes to estimate their diffusion times in the various animal or plant tissues, but I am sure that they don't act instantly.
X-from the diffusion point of view, epithelial and other mesothelial or endothelial cells lining tissue cavities would get fixed faster and better, because a large percentage of their surface is immediately exposed to the fixative. Ideally cells in suspension can be fixed instantly and then they can be observed under a microscope to provide a snapshot of live cells. Even so, what we see is far away from life. For instance, if you observe motile spermatozoa under light microscopy they look like snakes, but if you fix them they look more like spaghetti. And if this is true for cells like sperm that are prepared to come out of the body, what about a tissue we excise from an animal or during surgery and then we fix it. The cells in this tissue, deprived from their environment and blood supply, change rapidly and every single moment passing until final fixation counts.
I think that all these parameters together with the entire post-fixation preparation of the sample are of paramount importance when we make our microscopic observations and interpret our findings. In this sense the word "artifact" sounds a little bit like aphorism. We judge what images are closer to the "real" and ideal state we have in our mind (aesthetics are always involved) and we choose them to represent our findings. The rest of the images we reject, and we like to condemn them as artifacts or artefacts. In a way, we always choose something that we wish to see and we have to be very careful at this point. Pythagoras the philosopher forbade to his pupils to make wishes, on the grounds that they are too young and don't really know what to wish.
My apologies for much philosophy -we Greeks are good in this- and not providing references. I would love to hear yours and other colleagues' views on this subject.
Email: petereaton-at-hotmail.com Name: Peter Eaton
Organization: University of Porto, POrtugal
Title-Subject: [Filtered] Artifacts in aldehyde fixed cells
Message: Hi, I remember seeing some discussion (many years ago?), on the server about artifacts in microscopy of biological specimens in general. Specifically I think I read about membrane blebbing caused by aldehyde-based fixing. Does anyone have any actual references about this? I have seen what might be this effect, but it's hard to be sure. Thanks, Pete.
==============================Original Headers============================== 24, 25 -- From eikonika-at-otenet.gr Mon May 2 00:26:04 2011 24, 25 -- Received: from chimaera.otenet.gr (chimaera.otenet.gr [83.235.69.16]) 24, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p425Q3bX024309 24, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 00:26:04 -0500 24, 25 -- Received: from aiolos.otenet.gr (aiolos.otenet.gr [83.235.67.30]) 24, 25 -- by chimaera.otenet.gr (ESMTP) with ESMTP 24, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 08:26:02 +0300 (EEST) 24, 25 -- Received: from pegasus (athedsl-228738.home.otenet.gr [85.74.213.32]) 24, 25 -- by aiolos.otenet.gr (8.13.8/8.13.8/Debian-3) with SMTP id p425PwYk012214 24, 25 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 08:26:02 +0300 24, 25 -- Message-ID: {0C8A89B40DA94F34B3E1460CF3F19DAB-at-pegasus} 24, 25 -- From: "yorgos nikas" {eikonika-at-otenet.gr} 24, 25 -- To: {microscopy-at-microscopy.com} 24, 25 -- Subject: Artifacts in aldehyde fixed cells 24, 25 -- Date: Mon, 2 May 2011 08:25:51 +0300 24, 25 -- MIME-Version: 1.0 24, 25 -- Content-Type: text/plain; 24, 25 -- format=flowed; 24, 25 -- charset="iso-8859-7"; 24, 25 -- reply-type=original 24, 25 -- Content-Transfer-Encoding: 7bit 24, 25 -- X-Priority: 3 24, 25 -- X-MSMail-Priority: Normal 24, 25 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5843 24, 25 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5579 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 30 -- From PhillipsT-at-missouri.edu Mon May 2 06:50:26 2011 35, 30 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 35, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42BoQTS011991 35, 30 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 06:50:26 -0500 35, 30 -- X-IronPort-Anti-Spam-Filtered: true 35, 30 -- X-IronPort-Anti-Spam-Result: AlABAM2Zvk2Dl3nN/2dsb2JhbACYFA6Nene3GYhvAoMdgmEEhg6BDI92hik 35, 30 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 35, 30 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 02 May 2011 06:50:25 -0500 35, 30 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by 35, 30 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Mon, 2 May 2011 35, 30 -- 06:50:25 -0500 35, 30 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 35, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 35, 30 -- "petereaton-at-hotmail.com" {petereaton-at-hotmail.com} 35, 30 -- Date: Mon, 2 May 2011 06:50:24 -0500 35, 30 -- Subject: RE: [Microscopy] Artifacts in aldehyde fixed cells 35, 30 -- Thread-Topic: [Microscopy] Artifacts in aldehyde fixed cells 35, 30 -- Thread-Index: AcwIiYbLOTYHyN0/SQ2euiq6qnZhBQAM9dXA 35, 30 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF267BC0FAA0-at-UM-EMAIL05.um.umsystem.edu} 35, 30 -- References: {201105020526.p425QiqS025254-at-ns.microscopy.com} 35, 30 -- In-Reply-To: {201105020526.p425QiqS025254-at-ns.microscopy.com} 35, 30 -- Accept-Language: en-US 35, 30 -- Content-Language: en-US 35, 30 -- X-MS-Has-Attach: 35, 30 -- X-MS-TNEF-Correlator: 35, 30 -- acceptlanguage: en-US 35, 30 -- Content-Type: text/plain; charset="us-ascii" 35, 30 -- MIME-Version: 1.0 35, 30 -- Content-Transfer-Encoding: 8bit 35, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42BoQTS011991 ==============================End of - Headers==============================
Thanks to everyone who suggested ways to get the last bits of tissue off the scleral ossicles that I was trying to clean up. As I mentioned, I had trimmed the aldehyde-fixed tissue off these bones and then treated for a long time in sodium hydroxide which had removed much but not all the tissue. I thought collagen was one of the main remnants. Based on suggestions from several of you, I put them in Chlorox bleach. It certainly removed every trace of tissue. In fact, the imbricating bone plates (overlapping like roof tiles) all fell apart! The remaining plates are very white and brittle but I don't know if their fragility comes from the prolonged sodium hydroxide or too long treatment in Chlorox. Next time I will use shorter durations of each but, as I always tell my students, that's why we call them experiments. Thanks for the suggestions - I see success just over the horizon. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Ben, I didn't see any responses to this, so I'll give it a shot.
Basically, you've got to get rid of the oil. The beam isn't going to penetrate it worth a darn and, unless you're using a VP or ESEM, you're probably going to vaporize at least some of the oil and contaminate the whole chamber.
I would suggest getting an appropriate filter for the particle size and wash the oil away with an appropriate solvent, then put the washed (and maybe coated) filter in the SEM.
You didn't mention what the particles are made of or their size range, so there might possibly be some other options, but generally speaking SEMs don't deal with suspensions very well. The liquid stops the beam.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, April 30, 2011 3:45 PM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both benj-at-duke.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: benj-at-duke.edu Name: Ben
Organization: Duke university / CEE dpt.
Title-Subject: [Filtered] SEM of particles suspended in oil.
Message: Hello, I was wondering if someone had a method to visualize, with SEM, fine particles suspended in an oil phase. Let me know Thanks much Best Ben Login Host: 152.3.68.8 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 30 14:41:55 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3UJft52001833 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 5105938DB71 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id YXEy7EfcwYYc for {microscopy-at-microscopy.com} ; 7, 25 -- Sat, 30 Apr 2011 14:41:54 -0500 (CDT) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id CEED338DB66 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:54 -0500 (CDT) 7, 25 -- Message-ID: {4DBC6602.4090102-at-microscopy.com} 7, 25 -- Date: Sat, 30 Apr 2011 14:41:54 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: benj-at-duke.edu 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:SEM of particles suspended in oil 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 29 -- From kenconverse-at-qualityimages.biz Mon May 2 11:45:06 2011 21, 29 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42Gj6oE016404; 21, 29 -- Mon, 2 May 2011 11:45:06 -0500 21, 29 -- X-Authority-Analysis: v=1.1 cv=zBQrDHOGJVa+32FYvZQb0EuBWG+8UjOxCya+IIWDaxY= c=1 sm=0 a=ln1CGnfETAYA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=pzmDz54eAAAA:8 a=jU2bWg6BAAAA:8 a=emiYhC9mY8DBeq5eX7MA:9 a=9CZdg0ib7LTfs_EPfWAA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=Joj3jlGTlb8A:10 a=YC-Dtsxy4jEA:10 a=RyjLG6jaqdoA:10 a=AO_5y2s0I30A:10 a=bsRvvyHUn3IA:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 21, 29 -- X-Cloudmark-Score: 0 21, 29 -- X-Originating-IP: 72.224.149.57 21, 29 -- Received: from [72.224.149.57] ([72.224.149.57:2607] helo=Ken) 21, 29 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 21, 29 -- (ecelerity 2.2.3.46 r()) with ESMTP 21, 29 -- id F3/6A-29443-19FDEBD4; Mon, 02 May 2011 16:45:06 +0000 21, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 29 -- To: {microscopylistserver-noreply-at-microscopy.com} , 21, 29 -- "MSA Listserver" {microscopy-at-microscopy.com} 21, 29 -- Subject: RE: [Microscopy] viaWWW:SEM of particles suspended in oil 21, 29 -- Date: Mon, 2 May 2011 12:44:58 -0400 21, 29 -- Message-ID: {1EDB415A7A6445FB8E895AFF82C7C3BF-at-Ken} 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Type: text/plain; 21, 29 -- charset="us-ascii" 21, 29 -- X-Priority: 3 (Normal) 21, 29 -- X-MSMail-Priority: Normal 21, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 21, 29 -- In-Reply-To: {201104301945.p3UJj8IT015995-at-ns.microscopy.com} 21, 29 -- Importance: Normal 21, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 21, 29 -- Thread-Index: AcwHdJq0yurNwPlzQn6nnmVaG71LYABcm0gg 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42Gj6oE016404 ==============================End of - Headers==============================
Ben, An FYI about filters... In case you have not used them before, I highly recommend "Nuclepore" type membrane filters for SEM work. You don't have to contend with material being buried in the filter medium. An example can be found at: http://www.2spi.com/catalog/spec_prep/filter3.html No interest in SPI other than a customer.
Woody
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: Monday, May 02, 2011 12:52 PM To: WHITE Norvell (RS/IB)
Ben, I didn't see any responses to this, so I'll give it a shot.
Basically, you've got to get rid of the oil. The beam isn't going to penetrate it worth a darn and, unless you're using a VP or ESEM, you're probably going to vaporize at least some of the oil and contaminate the whole chamber.
I would suggest getting an appropriate filter for the particle size and wash the oil away with an appropriate solvent, then put the washed (and maybe coated) filter in the SEM.
You didn't mention what the particles are made of or their size range, so there might possibly be some other options, but generally speaking SEMs don't deal with suspensions very well. The liquid stops the beam.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, April 30, 2011 3:45 PM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both benj-at-duke.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: benj-at-duke.edu Name: Ben
Organization: Duke university / CEE dpt.
Title-Subject: [Filtered] SEM of particles suspended in oil.
Message: Hello, I was wondering if someone had a method to visualize, with SEM, fine particles suspended in an oil phase. Let me know Thanks much Best Ben Login Host: 152.3.68.8 ------------------------------------------------------------------------ ---
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 30 14:41:55 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3UJft52001833 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 5105938DB71 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id YXEy7EfcwYYc for {microscopy-at-microscopy.com} ; 7, 25 -- Sat, 30 Apr 2011 14:41:54 -0500 (CDT) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id CEED338DB66 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:54 -0500 (CDT) 7, 25 -- Message-ID: {4DBC6602.4090102-at-microscopy.com} 7, 25 -- Date: Sat, 30 Apr 2011 14:41:54 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: benj-at-duke.edu 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:SEM of particles suspended in oil 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 29 -- From kenconverse-at-qualityimages.biz Mon May 2 11:45:06 2011 21, 29 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 21, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42Gj6oE016404; 21, 29 -- Mon, 2 May 2011 11:45:06 -0500 21, 29 -- X-Authority-Analysis: v=1.1 cv=zBQrDHOGJVa+32FYvZQb0EuBWG+8UjOxCya+IIWDaxY= c=1 sm=0 a=ln1CGnfETAYA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=pzmDz54eAAAA:8 a=jU2bWg6BAAAA:8 a=emiYhC9mY8DBeq5eX7MA:9 a=9CZdg0ib7LTfs_EPfWAA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=Joj3jlGTlb8A:10 a=YC-Dtsxy4jEA:10 a=RyjLG6jaqdoA:10 a=AO_5y2s0I30A:10 a=bsRvvyHUn3IA:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 21, 29 -- X-Cloudmark-Score: 0 21, 29 -- X-Originating-IP: 72.224.149.57 21, 29 -- Received: from [72.224.149.57] ([72.224.149.57:2607] helo=Ken) 21, 29 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 21, 29 -- (ecelerity 2.2.3.46 r()) with ESMTP 21, 29 -- id F3/6A-29443-19FDEBD4; Mon, 02 May 2011 16:45:06 +0000 21, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 21, 29 -- To: {microscopylistserver-noreply-at-microscopy.com} , 21, 29 -- "MSA Listserver" {microscopy-at-microscopy.com} 21, 29 -- Subject: RE: [Microscopy] viaWWW:SEM of particles suspended in oil 21, 29 -- Date: Mon, 2 May 2011 12:44:58 -0400 21, 29 -- Message-ID: {1EDB415A7A6445FB8E895AFF82C7C3BF-at-Ken} 21, 29 -- MIME-Version: 1.0 21, 29 -- Content-Type: text/plain; 21, 29 -- charset="us-ascii" 21, 29 -- X-Priority: 3 (Normal) 21, 29 -- X-MSMail-Priority: Normal 21, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 21, 29 -- In-Reply-To: {201104301945.p3UJj8IT015995-at-ns.microscopy.com} 21, 29 -- Importance: Normal 21, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 21, 29 -- Thread-Index: AcwHdJq0yurNwPlzQn6nnmVaG71LYABcm0gg 21, 29 -- Content-Transfer-Encoding: 8bit 21, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42Gj6oE016404 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 32 -- From Woody.White-at-areva.com Mon May 2 13:39:26 2011 30, 32 -- Received: from ussmtpout2.areva.com (smtpout73.areva.com [160.84.253.73]) 30, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42IdQjr001448 30, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2011 13:39:26 -0500 30, 32 -- Received: by ussmtpout2.areva.com (Postfix, from userid 101) 30, 32 -- id B7E0C584D3; Mon, 2 May 2011 18:39:25 +0000 (UTC) 30, 32 -- Received: from RUSAHUBBX03.ad.corp (RUSAHUBBX03 [10.2.161.112]) 30, 32 -- by ussmtpout2.areva.com (Postfix) with ESMTP id B252A581FD 30, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2011 18:39:25 +0000 (UTC) 30, 32 -- Received: from auslyncbx03.adom.ad.corp ([160.84.193.81]) by RUSAHUBBX03.ad.corp with Microsoft SMTPSVC(6.0.3790.4675); 30, 32 -- Mon, 2 May 2011 14:39:25 -0400 30, 32 -- Received: from AUSLYNCMX02.adom.ad.corp ([160.84.193.181]) by auslyncbx03.adom.ad.corp with Microsoft SMTPSVC(6.0.3790.4675); 30, 32 -- Mon, 2 May 2011 14:39:25 -0400 30, 32 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 30, 32 -- Content-class: urn:content-classes:message 30, 32 -- MIME-Version: 1.0 30, 32 -- Content-Type: text/plain; 30, 32 -- charset="us-ascii" 30, 32 -- Subject: RE: [Microscopy] RE: viaWWW:SEM of particles suspended in oil 30, 32 -- Date: Mon, 2 May 2011 14:39:24 -0400 30, 32 -- Message-ID: {8D609EE2F807714CBF5297D9BA8602FB011B57D3-at-AUSLYNCMX02.adom.ad.corp} 30, 32 -- In-Reply-To: {201105021652.p42Gq6ta028073-at-ns.microscopy.com} 30, 32 -- X-MS-Has-Attach: 30, 32 -- X-MS-TNEF-Correlator: 30, 32 -- Thread-Topic: [Microscopy] RE: viaWWW:SEM of particles suspended in oil 30, 32 -- Thread-Index: AcwI6UZ6G5jdEIIMQjKRzKfqbEPlHQADj0Ng 30, 32 -- References: {201105021652.p42Gq6ta028073-at-ns.microscopy.com} 30, 32 -- From: "WHITE Norvell (AREVA)" {Woody.White-at-areva.com} 30, 32 -- To: {Microscopy-at-microscopy.com} 30, 32 -- X-OriginalArrivalTime: 02 May 2011 18:39:25.0201 (UTC) FILETIME=[42C4D810:01CC08F8] 30, 32 -- Content-Transfer-Encoding: 8bit 30, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42IdQjr001448 ==============================End of - Headers==============================
Both of our two SEMs are now VP-SEMs. One is a Hitachi and the other is an FEI. They are both good.
You certainly experience some loss of image resolution when operating in variable pressure mode. Some primary electrons scatter off of the residual gas, but most hit their intended target to give a good image.
As someone said, scattering can pose more of a problem with x-ray analysis. If 98% of the electrons hit their target, that means 2% still scatter. Suppose your sample is sparse particles of micron-sized chunks of pure A in a matrix of B. Those scattered electrons will mean you never find pure A. It will only ever be 98% pure with a 2% content of B. The effect will be proportional to pressure and it can be dealt with, but it will complicate analyses.
VP-SEMs can also be operated in high vacuum mode very similarly to what you are used to. However, I think you will find that the resolution lags just a little behind that of the high-vacuum SEMs. I recall that our Quanta-250 FEG was specified to have 1.2 nm resolution whereas the hivac SEM achieved a little better than 1 nm resolution. I understand that the detector choices are more limited for the VP-SEM than for the hi-vac SEM. In particular, I believe the "in-lens" detectors are not an option due to the design of a VP column. However, 1.2 nm is still pretty darn good in my opinion.
There is a bit of an extra cost for the fancier vacuum system compared to a conventional SEM with the same options. Personally, I prefer the flexibility that the VP-SEM gives and would go that way again.
Warren Straszheim Iowa State University
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, April 30, 2011 2:52 PM To: wesaia-at-iastate.edu
Hi Maya
I did a lot of this work many years ago. We used a copper- ferricyanide variant of the thiocholine technique (based on a Karnovsky and Roots paper 1964). Worked really well. The end plates become visible under a stereo microscope so can then be trimmed out easily for EM processing. They show up as little dark dots under the stereo microscope and in diaphragm they are really easy to find as they line up in a row across the diaphragm. Ultrastructure is fine. It was so successful we ended up doing this as a student practical for many years.
Check out chapter 4 of (including pages 223 to 228) Cytochemical Staining Methods for Electron Microscopy, PR Lewis and DP Knight, from the Audrey Glauert EM series (volume 14 I think).
My method is pre-computer but if you get stuck I can quickly type up method we used and send a copy to you.
Good luck
Allan
On 1/05/2011, at 7:57 AM, {microscopylistserver-noreply-at-microscopy.com} {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both yankova-at-neuron.uchc.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: yankova-at-neuron.uchc.edu Name: maya Yankova } } Title-Subject: [Filtered] Electron microscopy on motor end plates } } Message: Hello everyone, } } We have a researcher who wants to do EM on a motor endplate of a mouse } diaphragm. We would like to find a protocol to localize the motor } endplates on the muscle before the tissue is processed for EM. } Any advice would be greatly appreciated. } } Thanks } } Maya Yankova, M.Sc. } Research Assistant 3 } Central Electron Microscopy Facility } Department of Molecular, Microbial & Structural Biology } University of Connecticut Health Center } 263 Farmington Avenue } Farmington, CT 06030-3305 } ph: 860.679.2395 } yankova-at-neuron.uchc.edu }
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy and Structural Biology Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ NZ Microscopy Society: http://microscopynz.co.nz/
==============================Original Headers============================== 19, 23 -- From allan.mitchell-at-stonebow.otago.ac.nz Mon May 2 15:50:35 2011 19, 23 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 19, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42KoYgo002398 19, 23 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 15:50:35 -0500 19, 23 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 19, 23 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id p42KoWTA023287; 19, 23 -- Tue, 3 May 2011 08:50:32 +1200 19, 23 -- Received: from allan.otago.ac.nz (allan.otago.ac.nz [139.80.34.166]) 19, 23 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id 3D6434140893; 19, 23 -- Tue, 3 May 2011 08:50:32 +1200 (NZST) 19, 23 -- Subject: Re: [Microscopy] viaWWW:Electron microscopy on motor end plates 19, 23 -- Mime-Version: 1.0 (Apple Message framework v1084) 19, 23 -- Content-Type: text/plain; charset=us-ascii 19, 23 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 19, 23 -- In-Reply-To: {201104301957.p3UJvMcD027585-at-ns.microscopy.com} 19, 23 -- Date: Tue, 3 May 2011 08:50:32 +1200 19, 23 -- Cc: yankova-at-neuron.uchc.edu 19, 23 -- Message-Id: {900E0705-9E58-443E-8D82-DCF5D6E0C139-at-stonebow.otago.ac.nz} 19, 23 -- References: {201104301957.p3UJvMcD027585-at-ns.microscopy.com} 19, 23 -- To: microscopy-at-microscopy.com 19, 23 -- X-Mailer: Apple Mail (2.1084) 19, 23 -- Content-Transfer-Encoding: 8bit 19, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42KoYgo002398 ==============================End of - Headers==============================
I started a report sometime ago ( but sadly never finished it) about long term storage of tissue and while doing this came across the following papers. These may help.
Shelton, E. and Mowczko, W.E (1979) Scanning electron microscopy of membrane blisters produced by glutaraldehyde fixation and stablised by postfixation in Osmium Tetroxide, in: Freeze Fracture Methods, Artifacts and Interpretations, edited by J.E. Rash and C.S. Hudson. Raven Press , pp 67 - 69 **
Hay, E.D and Hasty, D.L (1979) Extrusion of particle free membrane blisters during glutaraldehyde fixation, in: Freeze Fracture Methods, Artifacts and Interpretations, edited by J.E. Rash and C.S. Hudson. Raven Press , pp 59 - 66
Hasty, D.L and Hay, E.D ., (1978) Freeze-fracture studies of the developing cell surface. 11. Particle free membrane blisters on glutaraldehyde-fixed corneal fibroblasts are artifacts. J. Cell Biology Vol 78: pp 756 - 768
Good luck
Allan
On 1/05/2011, at 7:57 AM, {microscopylistserver-noreply-at-microscopy.com} {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both petereaton-at-hotmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: petereaton-at-hotmail.com Name: Peter Eaton } } Organization: University of Porto, POrtugal } } Title-Subject: [Filtered] Artifacts in aldehyde fixed cells } } Message: Hi, } I remember seeing some discussion (many years ago?), on the server about } artifacts in microscopy of biological specimens in general. Specifically } I think I read about membrane blebbing caused by aldehyde-based fixing. } Does anyone have any actual references about this? I have seen what } might be this effect, but it's hard to be sure. Thanks, } Pete. } } Login Host: 193.136.24.81 } ---------------------------------------------------------------------------
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy and Structural Biology Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ NZ Microscopy Society: http://microscopynz.co.nz/
==============================Original Headers============================== 20, 23 -- From allan.mitchell-at-stonebow.otago.ac.nz Mon May 2 16:05:39 2011 20, 23 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 20, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42L5b7i018485 20, 23 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2011 16:05:38 -0500 20, 23 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 20, 23 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id p42L5ZJE030619; 20, 23 -- Tue, 3 May 2011 09:05:35 +1200 20, 23 -- Received: from allan.otago.ac.nz (allan.otago.ac.nz [139.80.34.166]) 20, 23 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id 39C104140D4D; 20, 23 -- Tue, 3 May 2011 09:05:35 +1200 (NZST) 20, 23 -- Subject: Re: [Microscopy] viaWWW:Artifacts in aldehyde fixed cells 20, 23 -- Mime-Version: 1.0 (Apple Message framework v1084) 20, 23 -- Content-Type: text/plain; charset=us-ascii 20, 23 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 20, 23 -- In-Reply-To: {201104301957.p3UJvMjg027595-at-ns.microscopy.com} 20, 23 -- Date: Tue, 3 May 2011 09:05:34 +1200 20, 23 -- Cc: petereaton-at-hotmail.com 20, 23 -- Message-Id: {D55156CE-6097-4334-87D6-7340BDD42DA9-at-stonebow.otago.ac.nz} 20, 23 -- References: {201104301957.p3UJvMjg027595-at-ns.microscopy.com} 20, 23 -- To: microscopy-at-microscopy.com 20, 23 -- X-Mailer: Apple Mail (2.1084) 20, 23 -- Content-Transfer-Encoding: 8bit 20, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42L5b7i018485 ==============================End of - Headers==============================
They do not give the more placid background of the NP filters, but they are impervious to most (probably all) solvents. Seems like this might be ideal to examine the particulates in oil since the particles would remain behind on the filters and the oil could be rinsed away with a solvent (like hexane).
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 20 -- From bozzola-at-siu.edu Mon May 2 16:21:05 2011 6, 20 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42LL5fD002254 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2011 16:21:05 -0500 6, 20 -- Received: by iyh42 with SMTP id 42so7099825iyh.0 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 02 May 2011 14:21:05 -0700 (PDT) 6, 20 -- MIME-Version: 1.0 6, 20 -- Received: by 10.42.147.137 with SMTP id n9mr178612icv.383.1304371265113; Mon, 6, 20 -- 02 May 2011 14:21:05 -0700 (PDT) 6, 20 -- Received: by 10.42.166.197 with HTTP; Mon, 2 May 2011 14:21:05 -0700 (PDT) 6, 20 -- In-Reply-To: {201105021840.p42IeAss002608-at-ns.microscopy.com} 6, 20 -- References: {201105021840.p42IeAss002608-at-ns.microscopy.com} 6, 20 -- Date: Mon, 2 May 2011 16:21:05 -0500 6, 20 -- Message-ID: {BANLkTimJL-bRB1N6bBQ1_E7P7-5c5BoU0g-at-mail.gmail.com} 6, 20 -- Subject: Re: [Microscopy] viaWWW:SEM of particles suspended in oil 6, 20 -- From: John Bozzola {bozzola-at-siu.edu} 6, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 20 -- Content-Type: text/plain; charset=UTF-8 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42LL5fD002254 ==============================End of - Headers==============================
They do not give the more placid background of the NP filters, but they are impervious to most (probably all) solvents. Seems like this might be ideal to examine the particulates in oil since the particles would remain behind on the filters and the oil could be rinsed away with a solvent (like hexane).
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, IL 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 20 -- From bozzola-at-siu.edu Mon May 2 16:21:05 2011 6, 20 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42LL5fD002254 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2011 16:21:05 -0500 6, 20 -- Received: by iyh42 with SMTP id 42so7099825iyh.0 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 02 May 2011 14:21:05 -0700 (PDT) 6, 20 -- MIME-Version: 1.0 6, 20 -- Received: by 10.42.147.137 with SMTP id n9mr178612icv.383.1304371265113; Mon, 6, 20 -- 02 May 2011 14:21:05 -0700 (PDT) 6, 20 -- Received: by 10.42.166.197 with HTTP; Mon, 2 May 2011 14:21:05 -0700 (PDT) 6, 20 -- In-Reply-To: {201105021840.p42IeAss002608-at-ns.microscopy.com} 6, 20 -- References: {201105021840.p42IeAss002608-at-ns.microscopy.com} 6, 20 -- Date: Mon, 2 May 2011 16:21:05 -0500 6, 20 -- Message-ID: {BANLkTimJL-bRB1N6bBQ1_E7P7-5c5BoU0g-at-mail.gmail.com} 6, 20 -- Subject: Re: [Microscopy] viaWWW:SEM of particles suspended in oil 6, 20 -- From: John Bozzola {bozzola-at-siu.edu} 6, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 20 -- Content-Type: text/plain; charset=UTF-8 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42LL5fD002254 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 36 -- From nizets2-at-yahoo.com Tue May 3 05:38:03 2011 19, 36 -- Received: from nm2-vm0.bullet.mail.ne1.yahoo.com (nm2-vm0.bullet.mail.ne1.yahoo.com [98.138.91.39]) 19, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p43Ac3H5029705 19, 36 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2011 05:38:03 -0500 19, 36 -- Received: from [98.138.90.54] by nm2.bullet.mail.ne1.yahoo.com with NNFMP; 03 May 2011 10:38:02 -0000 19, 36 -- Received: from [98.138.89.196] by tm7.bullet.mail.ne1.yahoo.com with NNFMP; 03 May 2011 10:38:02 -0000 19, 36 -- Received: from [127.0.0.1] by omp1054.mail.ne1.yahoo.com with NNFMP; 03 May 2011 10:38:02 -0000 19, 36 -- X-Yahoo-Newman-Property: ymail-3 19, 36 -- X-Yahoo-Newman-Id: 581714.53822.bm-at-omp1054.mail.ne1.yahoo.com 19, 36 -- Received: (qmail 32253 invoked by uid 60001); 3 May 2011 10:38:02 -0000 19, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1304419082; bh=gUdpqaUYUi3+ve+nMfSPCVqU9jtxgh+AT+zoq6Ea6PM=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=Hv1Hp6IsicRc6Hmq3IekRJVS0/d6B6eQE8SdC0u0x1rZpj+kfkxCGH0qeZGhZ5GEC9Z7JuFvVXwnDE7rVL5u32+Vo4k9yL2ZUZNY3hrLUInQH9H8nSdJMOWDnVEuzB89DhyVkR07+73CzX6xqjV6wyJD6fV2s2bmieYKNmxVV4I= 19, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 19, 36 -- s=s1024; d=yahoo.com; 19, 36 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 19, 36 -- b=IVW+bTYHplhbOYkppC0hkNhm78A3ROvPJNQZ1SDQA23E9o0aR9/l4fe6ApDR3+2aRJHob9AiWqzWGhq5+TH6iFyYwZFbgTCz174L7cZiyS69X1LxVXH+PdWhrCW+GpKBJYn1Y5sA+u37nHG6Rd4l9g/0DH2Y74V3AjEBe1/WETE=; 19, 36 -- Message-ID: {47893.13751.qm-at-web110804.mail.gq1.yahoo.com} 19, 36 -- X-YMail-OSG: IaW0mWQVM1m2FAantZ5HyH714ygKYKhUhCIuqYq3bhOmMbt 19, 36 -- VHgMyLuwod8yPR7EmVhT6jPRT5QWTNP8EubUgSMyBy32NeMWFafT1ke20Pq9 19, 36 -- WPsLiIKREpQ1gJYaDZDHz74iDi0wRkxR4x1F_5HXIGIoJn4U1hwRDcyfEGh0 19, 36 -- Njg3bK5X1a5JUYYWi4spo5I_z3gfM7BadIMSwZt3oYizRwBU3USnH_RiWAYH 19, 36 -- qoORDDcqnSYrMmiISUMONukBEhdfGSNqBh4HUOev6MsccVB_KZ7ZPOuVJ4yc 19, 36 -- 0aX66L_0NhwFFUD9OvKnurG8Hhv4v3bBmUGRwFt2h48foFmGoOsafdLlEfId 19, 36 -- cInIEWIiuczOcE8nRw0R1Rqrq4g1SAcOuIyVv98E0n9ItrBSwXjdfk8ElkYP 19, 36 -- 9RqHzvycPFTwW 19, 36 -- Received: from [80.122.101.100] by web110804.mail.gq1.yahoo.com via HTTP; Tue, 03 May 2011 03:38:01 PDT 19, 36 -- X-Mailer: YahooMailRC/559 YahooMailWebService/0.8.110.299900 19, 36 -- References: {201105022123.p42LNrPJ007948-at-ns.microscopy.com} 19, 36 -- Date: Tue, 3 May 2011 03:38:01 -0700 (PDT) 19, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} 19, 36 -- Subject: Re: [Microscopy] Re: viaWWW:SEM of particles suspended in oil 19, 36 -- To: microscopy-at-microscopy.com 19, 36 -- In-Reply-To: {201105022123.p42LNrPJ007948-at-ns.microscopy.com} 19, 36 -- MIME-Version: 1.0 19, 36 -- Content-Type: text/plain; charset=iso-8859-1 19, 36 -- Content-Transfer-Encoding: 8bit 19, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p43Ac3H5029705 ==============================End of - Headers==============================
Your problem has been informative and raises some anecdotes from my long-past youth that might be, at the least, philosophically interesting in the context of the varied processes used to prepare the harder parts of anatomy from their previous residents.
Two of the following three occurred while I was a graduate student (for six happy years - almost to the hour), and the other from earlier while doing my duty, 'standing in line waiting to ____ for my country' (as we used to put the time). The third occurred during my sojourn on the rifle range at 'Pleasure Island' while undergoing the trials of marksmanship.
The First occurred while on the range and demonstrating a disappointing level of incompetence, that I mused about how much better I would 'feel' if I were equipped, as a raptor, with an eye whose spericity was supported by bony plates. My older son, his sobriquet for the identical boot camp being "the bad place", came home with an 'Expert' badge while I had achieved that which he and his other extremely qualified marksmen called a 'toilet seat'.
The second occurred while as a graduate student, I was taking care of the department timber rattlesnake. I fed the beast from our mouse colony and cleaned the cage of her/his leavings which always included perfectly cleaned mouse skulls and other parts of the skeleton. On one occasion, I was forced by circumstance to ask an undergraduate 'intern' to perform the feeding while I was away learning the initial lessons of matrimonial bliss. When I returned, all appeared to be well, and I resumed my normal intellectual and husbanding responsibilities. When it came time for the next housekeeping chores in the reptile's cage, I found the usual skeletal remains and one perfectly preserved bicornate uterus sans all signs of the pregnancy of the previous offering provided by my substitute. Thus, I earned a piece of nickel-knowledge, that has survived to this day; for, there had been, when I returned from my 'vacation', a missing pregnant female, which I had been led to believe was among a group of mice that had been found deceased. Further interrogation of the undergraduate disclosed that the mouse he had provide the snake had been 'a real fat one'. Thus, it was clear, without need for further experiments that a pregnant uterus was quite distinct from a non-pregnant one. Interestingly, the uterus was resistant of every attempt to soften it for sectioning. It appeared to have been very nicely tanned.
The third occurred during my last summer when my wife and I resided at my fraternity house. Over the course of two days, the fraternity mascot - a goat - was found dead in its pen (we HAD fed and watered it) and I was provided with the body of a feral cat which was gaunt and obviously had died as a nursing mother. I had two reasons for determining to have skeletons of both of these animals - the goat, so the fraternity would not lack for its mascot, and the cat, because I wanted to have a skeleton from a feral, and very emaciated, feline to compare with my other which was acquired from a healthy deceased animal from the local 'SPCA'. Her skeleton appeared to be from an animal with very poor nutrition. All of the bones of the body were covered with a white, chalky dust, and over the almost thirty years I had them, they never lost their dusty texture. The dermal bones of the skull were especially delicate though they never wore through. Flakes of apatite were always to be found in the box, no matter how often I cleaned it out.
Except for the factual nature of these anecdotes, they would readily be recognized as 'sea stories' by any marine.
Cheers, and that's the truth,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] Sent: Monday, May 02, 2011 9:59 AM To: Monson, Frederick
Thanks to everyone who suggested ways to get the last bits of tissue off the scleral ossicles that I was trying to clean up. As I mentioned, I had trimmed the aldehyde-fixed tissue off these bones and then treated for a long time in sodium hydroxide which had removed much but not all the tissue. I thought collagen was one of the main remnants. Based on suggestions from several of you, I put them in Chlorox bleach. It certainly removed every trace of tissue. In fact, the imbricating bone plates (overlapping like roof tiles) all fell apart! The remaining plates are very white and brittle but I don't know if their fragility comes from the prolonged sodium hydroxide or too long treatment in Chlorox. Next time I will use shorter durations of each but, as I always tell my students, that's why we call them experiments. Thanks for the suggestions - I see success just over the horizon. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
Hi Ben, I did a lot of work on soap structures in grease and that means to get rid of the oil in the structure... See 9th image at my website: http://www.elektronenmikroskopie.info/galerie%20-%20rem-material.htm
Best (if you like to do EDS also) to use an aluminium stub, polish it down to 250nm roughness. Add a drop of your solution of the suspended particles to the stub, dry under infrared light. It might be best to dilute the oil in pet ether first... Then carefully drop 40° petroleum ether from one side on the tilted stub to wash away the oil. Do this ca. 5-10 times. Then coat as needed. With a chromium coating I came down to a resolution of ca. 2-3 nm... :-)
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Wissenschaftliche Photographie Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 30.04.11 21:49, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both benj-at-duke.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: benj-at-duke.edu Name: Ben } } Organization: Duke university / CEE dpt. } } Title-Subject: [Filtered] SEM of particles suspended in oil. } } Message: Hello, } I was wondering if someone had a method to visualize, with SEM, fine } particles suspended in an oil phase. } Let me know } Thanks much } Best } Ben } Login Host: 152.3.68.8 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Apr 30 14:41:55 2011 } 7, 25 -- Received: from znl.com ([206.69.208.20]) } 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p3UJft52001833 } 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 } 7, 25 -- Received: from localhost (localhost [127.0.0.1]) } 7, 25 -- by znl.com (Postfix) with ESMTP id 5105938DB71 } 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:55 -0500 (CDT) } 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 7, 25 -- Received: from znl.com ([127.0.0.1]) } 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 7, 25 -- with ESMTP id YXEy7EfcwYYc for {microscopy-at-microscopy.com} ; } 7, 25 -- Sat, 30 Apr 2011 14:41:54 -0500 (CDT) } 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 7, 25 -- by znl.com (Postfix) with ESMTPA id CEED338DB66 } 7, 25 -- for {microscopy-at-microscopy.com} ; Sat, 30 Apr 2011 14:41:54 -0500 (CDT) } 7, 25 -- Message-ID: {4DBC6602.4090102-at-microscopy.com} } 7, 25 -- Date: Sat, 30 Apr 2011 14:41:54 -0500 } 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 7, 25 -- Reply-To: benj-at-duke.edu } 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 7, 25 -- MIME-Version: 1.0 } 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 25 -- Subject: viaWWW:SEM of particles suspended in oil } 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 23 -- From stefan.diller-at-t-online.de Tue May 3 11:52:05 2011 10, 23 -- Received: from mailout06.t-online.de (mailout06.t-online.de [194.25.134.19]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p43Gq4na003536 10, 23 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2011 11:52:04 -0500 10, 23 -- Received: from fwd19.aul.t-online.de (fwd19.aul.t-online.de ) 10, 23 -- by mailout06.t-online.de with smtp 10, 23 -- id 1QHIpO-0007Cf-P9; Tue, 03 May 2011 18:52:02 +0200 10, 23 -- Received: from mac-pro.speedport.ip (TQO01aZerhZVlEhBC9p26GotdW6i+2DGfTj2d5YTK4uUVnr3DXJGkRDhUGAbJRygqV-at-[93.222.115.219]) by fwd19.t-online.de 10, 23 -- with esmtp id 1QHIpL-1Of9eK0; Tue, 3 May 2011 18:51:59 +0200 10, 23 -- Message-ID: {4DC032AE.90604-at-t-online.de} 10, 23 -- Date: Tue, 03 May 2011 18:51:58 +0200 10, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 10, 23 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 10, 23 -- MIME-Version: 1.0 10, 23 -- To: microscopy-at-microscopy.com 10, 23 -- Subject: Re: [Microscopy] viaWWW:SEM of particles suspended in oil 10, 23 -- References: {201104301949.p3UJnWtO031431-at-ns.microscopy.com} 10, 23 -- In-Reply-To: {201104301949.p3UJnWtO031431-at-ns.microscopy.com} 10, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 23 -- X-ID: TQO01aZerhZVlEhBC9p26GotdW6i+2DGfTj2d5YTK4uUVnr3DXJGkRDhUGAbJRygqV 10, 23 -- X-TOI-MSGID: 6bbd233b-a8cd-4258-b5c7-9ef66a23e444 10, 23 -- Content-Transfer-Encoding: 8bit 10, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p43Gq4na003536 ==============================End of - Headers==============================
I posted a message last week about a colleague who has a functional Philips 300 TEM. He is now looking for a outfit to remove the unit in short order. It is located at Providence College in Providence, RI.
Please contact him directly as he is not on this list. Joseph A DeGiorgis, PhD Biology Department Providence College Providence, RI 02 918-0001 jdegiorg-at-providence.edu 508-292-4605 (Cell)
Thanks, Louie
-- Louis Kerr lkerr-at-mbl.edu
Research and Education Support Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543 508-289-7273 508-540-6902 (FAX) 508-292-0289 (Cell phone)
VISIT OUR WEB SITES: http://www.mbl.edu/ http://www.courses.mbl.edu/
==============================Original Headers============================== 7, 31 -- From lkerr-at-mbl.edu Tue May 3 14:53:48 2011 7, 31 -- Received: from mailfrontier.mbl.edu (mailfrontier.mbl.edu [128.128.172.17]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p43JrlnN004645 7, 31 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2011 14:53:47 -0500 7, 31 -- Received: from mailfrontier.mbl.edu (127.0.0.1) by mailfrontier.mbl.edu (MlfMTA v3.2r9) id ho1ekm0171sv for {microscopy-at-microscopy.com} ; Tue, 3 May 2011 15:53:47 -0400 (envelope-from {lkerr-at-mbl.edu} ) 7, 31 -- Received: from mail.mbl.edu ([128.128.172.47]) 7, 31 -- by mailfrontier.mbl.edu (SonicWALL 7.2.1.2841) 7, 31 -- with ESMTP; Tue, 03 May 2011 15:53:47 -0400 7, 31 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 31 -- by mail.mbl.edu (Postfix) with ESMTP id 4628A1824002 7, 31 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2011 15:53:47 -0400 (EDT) 7, 31 -- X-Virus-Scanned: amavisd-new at mbl.edu 7, 31 -- Received: from mail.mbl.edu ([127.0.0.1]) 7, 31 -- by localhost (mail.mbl.edu [127.0.0.1]) (amavisd-new, port 10024) 7, 31 -- with ESMTP id jyM9LOWeRDAC for {microscopy-at-microscopy.com} ; 7, 31 -- Tue, 3 May 2011 15:53:46 -0400 (EDT) 7, 31 -- Received: from mail.mbl.edu (mail.mbl.edu [128.128.172.47]) 7, 31 -- by mail.mbl.edu (Postfix) with ESMTP id DA688173007E 7, 31 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2011 15:53:46 -0400 (EDT) 7, 31 -- Date: Tue, 3 May 2011 15:53:46 -0400 (EDT) 7, 31 -- From: Louis Kerr {lkerr-at-mbl.edu} 7, 31 -- To: Microscopy {microscopy-at-microscopy.com} 7, 31 -- Message-ID: {940757951.348368.1304452426799.JavaMail.root-at-mail.mbl.edu} 7, 31 -- Subject: Removal of a Philips 300 TEM 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Type: text/plain; charset=utf-8 7, 31 -- Content-Transfer-Encoding: 7bit 7, 31 -- X-Originating-IP: [10.10.5.235] 7, 31 -- X-Mailer: Zimbra 6.0.8_GA_2685 (ZimbraWebClient - FF3.0 (Mac)/6.0.8_GA_2685) 7, 31 -- X-Mlf-Version: 7.2.1.2841 7, 31 -- X-Mlf-UniqueId: o201105031953470037650 ==============================End of - Headers==============================
Dear Colleagues, On behalf of the meeting organizers I am pleased to invite you to attend the X Brazilian MRS Meeting (in special Symposium M: Advances and Applications of Electron Microscopy), which will be held in Gramado-RS-Brazil, in September 25-29. The submission deadline is 13th, may.
More information about Symposium M can be found at: http://www.sbpmat.org.br/10encontro/
Your attending will be very helpful to the success of the conference. We hope see you at Brazilian MRS Conference.
Best regards, Prof. Dr. Marcelo O. Orlandi Symposium Organizer
--------------------------------------------------- Esta mensagem foi enviada pelo WebMail do Instituto de Quimica de Araraquara-SP (UNESP)
==============================Original Headers============================== 7, 21 -- From orlandi-at-iq.unesp.br Tue May 3 15:57:38 2011 7, 21 -- Received: from mail.iq.unesp.br (mail.iq.unesp.br [200.145.86.145]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p43KvbX1021276 7, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 3 May 2011 15:57:38 -0500 7, 21 -- Received: by mail.iq.unesp.br (Postfix, from userid 48) 7, 21 -- id 80EAB13C0FA; Tue, 3 May 2011 17:54:40 -0300 (BRT) 7, 21 -- Received: from 200.145.90.190 ([200.145.90.190]) by mail.iq.unesp.br (Horde 7, 21 -- MIME library) with HTTP; Tue, 03 May 2011 17:54:40 -0300 7, 21 -- Message-ID: {20110503175440.c8fl60gqn4kgwc0s-at-mail.iq.unesp.br} 7, 21 -- Date: Tue, 03 May 2011 17:54:40 -0300 7, 21 -- From: orlandi-at-iq.unesp.br 7, 21 -- To: Microscopy-at-microscopy.com 7, 21 -- Subject: Brazilian MRS 7, 21 -- MIME-Version: 1.0 7, 21 -- Content-Type: text/plain; 7, 21 -- charset=ISO-8859-1; 7, 21 -- DelSp="Yes"; 7, 21 -- format="flowed" 7, 21 -- Content-Disposition: inline 7, 21 -- Content-Transfer-Encoding: 7bit 7, 21 -- User-Agent: Webmail STI/IQ - vr 1.0 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both travisj25-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: travisj25-at-gmail.com Name: Travis Johnson
Title-Subject: [Filtered] EDS - Electron Beam Excitation
Message: We are working with "Quantitative Analysis Software" (QAS) for an EDS detector. The software is used both for auto identification of peaks and for quantitation. The software was apparently developed for use with an X-ray excitation source but may be usable also for electron beam excitation. In our case the electron beam is a cold cathode source with a nominal beam energy of 20 kV. Because of the nature of the cold cathode electron beam source, there is an appreciable energy spread in the exciting beam (as contrasted with more elaborate sources on SEMs, etc.)
The samples for the most part are geological (rock thin sections and thin slabs).
If anyone has any experience or references to work in this area, we would be pleased to hear about them.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both y.han.1-at-bham.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: y.han.1-at-bham.ac.uk Name: Yisong Han
Organization: University of Birmingham
Title-Subject: [Filtered] EDX
Message: Dear All,
We planned to buy an SDD EDX for our JEOL 2100f probe corrected TEM with UHR pole pieces. We can go with either an Oxford X-MAX or a Bruker. I am wondering if anyone could comment on these two systems in terms of ease of use, reliability, sensitivity, compatibility with JEOL machines, etc. Thanks very much in advance.
I have been using SEMEDS for 30 years, but most of the time was spent on (obviously) early systems. I have had the Oxford system for two years. Having used only the X-Max SDD detector, I cannot offer a firsthand comparison to Bruker. I can say I am quite satisfied with the X-Max detector on my Zeiss SIGMA SEM. Sensitivity down to Boron is what one would expect of a premium detector. Count rates are good and after some initial (at install, fixed) problems with a spurious peak, operation has been trouble free.
I am not quite as happy with the INCA ENERGY software. It performs very well but it is somewhat inflexible, limiting how it is used (This is vague, I know, but there are too many small details to describe here). To be fair, as prefaced, it does a good job, just not the way I would always prefer. I understand that new software is being (has been?) released and may well address the situation. The new software is also supposed to be Windows7/64 bit compatible.
Woody
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both y.han.1-at-bham.ac.uk as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: y.han.1-at-bham.ac.uk Name: Yisong Han
Organization: University of Birmingham
Title-Subject: [Filtered] EDX
Message: Dear All,
We planned to buy an SDD EDX for our JEOL 2100f probe corrected TEM with UHR pole pieces. We can go with either an Oxford X-MAX or a Bruker. I am wondering if anyone could comment on these two systems in terms of ease of use, reliability, sensitivity, compatibility with JEOL machines, etc.
Does anyone know why the Aluminium oxide filters are discontinued? Also is it possible to clean the Silver filters?
Thanks Kahlee
Kahlee Redman Senior Forensic Scientist
Note: My work days are Monday, Wednesday and Thursday. I shall respond to your request as soon as possible. If your matter requires urgent attention please contact Hayley Brown at FSSA.
Forensic Science SA 21 Divett Place, SA 5000 Phone: + 61 8 82267700 Fax: +61 8 82267777 email: kahlee.redman-at-sa.gov.au
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Tuesday, 3 May 2011 6:58 AM To: Redman, Kahlee (AGD)
In addition to the Nucleopore filters mentioned earlier by Woody White, you might consider using either silver or aluminum oxide micro-filters:
They do not give the more placid background of the NP filters, but they are impervious to most (probably all) solvents. Seems like this might be ideal to examine the particulates in oil since the particles would remain behind on the filters and the oil could be rinsed away with a solvent (like hexane).
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 20 -- From bozzola-at-siu.edu Mon May 2 16:21:05 2011 6, 20 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p42LL5fD002254 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2011 16:21:05 -0500 6, 20 -- Received: by iyh42 with SMTP id 42so7099825iyh.0 6, 20 -- for {Microscopy-at-microscopy.com} ; Mon, 02 May 2011 14:21:05 -0700 (PDT) 6, 20 -- MIME-Version: 1.0 6, 20 -- Received: by 10.42.147.137 with SMTP id n9mr178612icv.383.1304371265113; Mon, 6, 20 -- 02 May 2011 14:21:05 -0700 (PDT) 6, 20 -- Received: by 10.42.166.197 with HTTP; Mon, 2 May 2011 14:21:05 -0700 (PDT) 6, 20 -- In-Reply-To: {201105021840.p42IeAss002608-at-ns.microscopy.com} 6, 20 -- References: {201105021840.p42IeAss002608-at-ns.microscopy.com} 6, 20 -- Date: Mon, 2 May 2011 16:21:05 -0500 6, 20 -- Message-ID: {BANLkTimJL-bRB1N6bBQ1_E7P7-5c5BoU0g-at-mail.gmail.com} 6, 20 -- Subject: Re: [Microscopy] viaWWW:SEM of particles suspended in oil 6, 20 -- From: John Bozzola {bozzola-at-siu.edu} 6, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 20 -- Content-Type: text/plain; charset=UTF-8 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p42LL5fD002254 ==============================End of - Headers==============================
==============================Original Headers============================== 21, 30 -- From Kahlee.Redman-at-sa.gov.au Wed May 4 19:06:40 2011 21, 30 -- Received: from mailgate9.sa.gov.au (mailgate9.sa.gov.au [203.6.147.11]) 21, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4506b4q012965 21, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 4 May 2011 19:06:39 -0500 21, 30 -- X-IronPort-AV: E=Sophos;i="4.64,317,1301841000"; 21, 30 -- d="scan'208";a="55404221" 21, 30 -- Received: from unknown (HELO EMSWM302.sagemsmrd01.sa.gov.au) ([10.154.32.68]) 21, 30 -- by mailgate9.sa.gov.au with ESMTP/TLS/RC4-MD5; 05 May 2011 09:36:29 +0930 21, 30 -- Received: from EMSGM101.sagemsmrd01.sa.gov.au (10.154.32.57) by 21, 30 -- EMSWM302.sagemsmrd01.sa.gov.au (10.154.32.68) with Microsoft SMTP Server 21, 30 -- (TLS) id 8.3.106.1; Thu, 5 May 2011 09:36:29 +0930 21, 30 -- Received: from EMSCM005.sagemsmrd01.sa.gov.au ([10.154.32.26]) by 21, 30 -- EMSGM101.sagemsmrd01.sa.gov.au ([10.154.32.57]) with mapi; Thu, 5 May 2011 21, 30 -- 09:36:28 +0930 21, 30 -- From: "Redman, Kahlee (AGD)" {Kahlee.Redman-at-sa.gov.au} 21, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 30 -- Date: Thu, 5 May 2011 09:36:28 +0930 21, 30 -- Subject: FW: [Microscopy] Re: viaWWW:SEM of particles suspended in oil 21, 30 -- Thread-Topic: [Microscopy] Re: viaWWW:SEM of particles suspended in oil 21, 30 -- Thread-Index: AcwJD+Qt56neXOYJSB2gZmswdhmKWgBqDRBQ 21, 30 -- Message-ID: {CB27E4164EE17744AC24B7250BFE86341D24FEDB65-at-EMSCM005.sagemsmrd01.sa.gov.au} 21, 30 -- Accept-Language: en-US, en-AU 21, 30 -- Content-Language: en-US 21, 30 -- X-MS-Has-Attach: 21, 30 -- X-MS-TNEF-Correlator: 21, 30 -- acceptlanguage: en-US, en-AU 21, 30 -- Content-Type: text/plain; charset="iso-8859-1" 21, 30 -- MIME-Version: 1.0 21, 30 -- Content-Transfer-Encoding: 8bit 21, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4506b4q012965 ==============================End of - Headers==============================
In doing a literature search I came across a paper recommending the use of polyvinylpyrrolidone in glutaraldehyde fixatives to help minimize changes due to osmotic pressure. In our case we would be using it on mouse intestinal tissue.
J. ULTRASTRUCTORE RESEARCH 30, 195-208 (1970) Effects on Tissue Fine Structure of Variations in Colloid Osmotic Pressure of Glutaraldehyde Fixatives ~ SVEN-OLOF BOHMAN AND ARVID B. MAUNSBACH
We often do add sucrose to our fix for osmotic control but I have never used PVP. I would appreciate comments from those who might have experience using it.
Thanks in advance Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 9, 28 -- From dsherman-at-purdue.edu Wed May 4 20:44:10 2011 9, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p451iAqv030449 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2011 20:44:10 -0500 9, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 9, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p451i9Cg007719 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2011 21:44:09 -0400 9, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.138]) by WPPEXHUB02F.purdue.lcl 9, 28 -- ([::1]) with mapi; Wed, 4 May 2011 21:44:09 -0400 9, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 9, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 9, 28 -- Date: Wed, 4 May 2011 21:44:08 -0400 9, 28 -- Subject: Use of polyvinylpyrrolidone in fix 9, 28 -- Thread-Topic: Use of polyvinylpyrrolidone in fix 9, 28 -- Thread-Index: AcwKxeyGuFoqfvEo90unkHOHZiq7Lw== 9, 28 -- Message-ID: {C9E77928.E40D%dsherman-at-purdue.edu} 9, 28 -- Accept-Language: en-US 9, 28 -- Content-Language: en-US 9, 28 -- X-MS-Has-Attach: 9, 28 -- X-MS-TNEF-Correlator: 9, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 9, 28 -- acceptlanguage: en-US 9, 28 -- Content-Type: text/plain; charset="us-ascii" 9, 28 -- MIME-Version: 1.0 9, 28 -- X-PMX-Version: 5.5.9.388399 9, 28 -- X-PerlMx-Virus-Scanned: Yes 9, 28 -- Content-Transfer-Encoding: 8bit 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p451iAqv030449 ==============================End of - Headers==============================
Research Fellow – Electron Microscopy (fixed term)
Job No: 495814 Location: Department of Materials Engineering, Monash University, (Clayton campus, Melbourne, Australia) Salary: The appointment level will depend upon qualifications and experience: $74,032 - $79,469 Level A (includes 9% superannuation) $83,651 - $99,337 Level B (includes 9% superannuation) Duration: Up to three years fixed-term appointment.
The Department of Materials Engineering at Monash University, in Melbourne, is seeking to appoint an outstanding Research Fellow with expertise in the area of aberration-corrected transmission electron microscopy. The Research Fellow will conduct high-level research in theoretical and/or experimental aspects of aberration-corrected STEM EELS and associated techniques in relation to the research project "Chemical mapping of materials at the atomic scale". The Fellow will work closely with the research team and instruments at the Monash Centre for Electron Microscopy. The Centre has a world-class suite of instrumentation, including a double-aberration-corrected Titan3 80-300kV FEG-TEM.
The successful applicant will have advanced understanding and skills in the theory and application of TEM and a PhD or equivalent qualification and will possess sound interpersonal skills, along with excellent written and oral communication skills.
Full details and instructions on how to apply can be found at: http://jobs.monash.edu.au/jobDetails.asp?sJobIDs=495814
Enquiries can be made to: Associate Professor Joanne Etheridge, Monash Centre for Electron Microscopy, Department of Materials Engineering on, Tel.: +61 (0) 3 9905 1836 Fax: +61 (0) 3 9905 3600 Email: joanne.etheridge-at-monash.edu
==============================Original Headers============================== 11, 23 -- From christian.dwyer-at-monash.edu Thu May 5 02:13:24 2011 11, 23 -- Received: from na3sys009aog113.obsmtp.com (na3sys009aog113.obsmtp.com [74.125.149.209]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p457DO4j027092 11, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 02:13:24 -0500 11, 23 -- Received: from mail-pv0-f176.google.com ([74.125.83.176]) (using TLSv1) by na3sys009aob113.postini.com ([74.125.148.12]) with SMTP 11, 23 -- ID DSNKTcJOE/UvEtW3lOZlobWV45TaFsTP+2RR-at-postini.com; Thu, 05 May 2011 00:13:24 PDT 11, 23 -- Received: by pve37 with SMTP id 37so1128721pve.21 11, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 05 May 2011 00:13:23 -0700 (PDT) 11, 23 -- Received: by 10.68.33.40 with SMTP id o8mr1580420pbi.370.1304579603117; 11, 23 -- Thu, 05 May 2011 00:13:23 -0700 (PDT) 11, 23 -- Received: from dyn-130-194-93-16.its.monash.edu.au (dyn-130-194-93-16.its.monash.edu.au [130.194.93.16]) 11, 23 -- by mx.google.com with ESMTPS id f4sm1252662pbs.36.2011.05.05.00.13.20 11, 23 -- (version=TLSv1/SSLv3 cipher=OTHER); 11, 23 -- Thu, 05 May 2011 00:13:22 -0700 (PDT) 11, 23 -- Message-ID: {4DC24E14.7010402-at-monash.edu} 11, 23 -- Date: Thu, 05 May 2011 17:13:24 +1000 11, 23 -- From: Christian Dwyer {christian.dwyer-at-monash.edu} 11, 23 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.4; en-US; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 11, 23 -- MIME-Version: 1.0 11, 23 -- To: Microscopy-at-microscopy.com 11, 23 -- Subject: Postdoctoral Position Available - Aberration-Corrected STEM 11, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed 11, 23 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
If anyone has an old Gatan PEELS 666 they don't want anymore or it has ceased to function. I would like the rear aluminium assembly that supports the detector (post phosphor) and also supplies the cooling water. Detector and electronics don't need to work. I just want the aluminium fitting to modify. I'm happy to pay for shipping.
I have images of the part in question, please contact me offline and I can send them through so we are comparing apples with apples.
Thank you in advance
Regards George
==============================Original Headers============================== 4, 28 -- From george.theodossiou-at-st-annes.ox.ac.uk Thu May 5 08:53:31 2011 4, 28 -- Received: from relay5.mail.ox.ac.uk (relay5.mail.ox.ac.uk [163.1.2.163]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45DrVaF016810 4, 28 -- for {Microscopy-at-Microscopy.com} ; Thu, 5 May 2011 08:53:31 -0500 4, 28 -- Received: from smtp3.nexus.ox.ac.uk ([163.1.154.137] helo=exht03.ad.oak.ox.ac.uk) 4, 28 -- by relay5.mail.ox.ac.uk with esmtp (Exim 4.75) 4, 28 -- (envelope-from {george.theodossiou-at-st-annes.ox.ac.uk} ) 4, 28 -- id 1QHyzi-0003FE-JC 4, 28 -- for Microscopy-at-Microscopy.com; Thu, 05 May 2011 14:53:30 +0100 4, 28 -- Received: from EXMBX01.ad.oak.ox.ac.uk ([169.254.1.140]) by 4, 28 -- exht03.ad.oak.ox.ac.uk ([163.1.154.54]) with mapi; Thu, 5 May 2011 14:53:30 4, 28 -- +0100 4, 28 -- From: George Theodossiou {george.theodossiou-at-st-annes.ox.ac.uk} 4, 28 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 4, 28 -- Date: Thu, 5 May 2011 14:51:00 +0100 4, 28 -- Subject: Gatan PEELS 666 - Parts 4, 28 -- Thread-Topic: Gatan PEELS 666 - Parts 4, 28 -- Thread-Index: AQHMCyvQf7i2tx1kgUiGAU85fEyI7A== 4, 28 -- Message-ID: {CFADFFD51D0744438FC8E1A5A990E4DF89266080B2-at-EXMBX01.ad.oak.ox.ac.uk} 4, 28 -- Accept-Language: en-US, en-GB 4, 28 -- Content-Language: en-GB 4, 28 -- X-MS-Has-Attach: 4, 28 -- X-MS-TNEF-Correlator: 4, 28 -- acceptlanguage: en-US, en-GB 4, 28 -- Content-Type: text/plain; charset="us-ascii" 4, 28 -- MIME-Version: 1.0 4, 28 -- Content-Transfer-Encoding: 8bit 4, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45DrVaF016810 ==============================End of - Headers==============================
I used PVP many, many years ago when I was doing TEM on the renal medulla, very hypertonic down there. How much the PVP helped versus how much adding lots of NaCl to the fix to make it hypertonic helped is open to question. I got the recipe from Bohman's work on the kidney which had very nice EMs. J. Ultrastruc. Res. 47:329-360, 1974. I actually met him some years later, nice guy and a good scientist. I have also done TEM of mouse duodenum and never had a problem getting good fixation with 2.5% glut in buffer. I think I used 0.08M cacodylate in those days but probably phosphate as well. I did not perfuse but just flushed the lumen with fix from a small syringe with a 25g needle. Final answer, it is probably not necessary but there is enough small intestine in a mouse to try adjacent pieces of tissue in fix with and without PVP.
Geoff
On 5/4/2011 9:44 PM, dsherman-at-purdue.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } In doing a literature search I came across a paper recommending the use of } polyvinylpyrrolidone in glutaraldehyde fixatives to help minimize changes } due to osmotic pressure. In our case we would be using it on mouse } intestinal tissue. } } J. ULTRASTRUCTORE RESEARCH 30, 195-208 (1970) } Effects on Tissue Fine Structure of Variations in Colloid } Osmotic Pressure of Glutaraldehyde Fixatives ~ } SVEN-OLOF BOHMAN AND ARVID B. MAUNSBACH } } We often do add sucrose to our fix for osmotic control but I have never used } PVP. I would appreciate comments from those who might have experience using } it. } } Thanks in advance } Debby } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy } } } } } ==============================Original Headers============================== } 9, 28 -- From dsherman-at-purdue.edu Wed May 4 20:44:10 2011 } 9, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p451iAqv030449 } 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2011 20:44:10 -0500 } 9, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) } 9, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p451i9Cg007719 } 9, 28 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2011 21:44:09 -0400 } 9, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.138]) by WPPEXHUB02F.purdue.lcl } 9, 28 -- ([::1]) with mapi; Wed, 4 May 2011 21:44:09 -0400 } 9, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 9, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 9, 28 -- Date: Wed, 4 May 2011 21:44:08 -0400 } 9, 28 -- Subject: Use of polyvinylpyrrolidone in fix } 9, 28 -- Thread-Topic: Use of polyvinylpyrrolidone in fix } 9, 28 -- Thread-Index: AcwKxeyGuFoqfvEo90unkHOHZiq7Lw== } 9, 28 -- Message-ID: {C9E77928.E40D%dsherman-at-purdue.edu} } 9, 28 -- Accept-Language: en-US } 9, 28 -- Content-Language: en-US } 9, 28 -- X-MS-Has-Attach: } 9, 28 -- X-MS-TNEF-Correlator: } 9, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 9, 28 -- acceptlanguage: en-US } 9, 28 -- Content-Type: text/plain; charset="us-ascii" } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- X-PMX-Version: 5.5.9.388399 } 9, 28 -- X-PerlMx-Virus-Scanned: Yes } 9, 28 -- Content-Transfer-Encoding: 8bit } 9, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p451iAqv030449 } ==============================End of - Headers============================== } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
I think I may have asked this before. What are your ideas about the shelf life of embedding resins?
We have a bunch, some very old (a few back to 1983), and I don't know what to do with them. Are they good to use or should they just go?
I know I could mix up test sets, but I'm not sure I can spare the time. Plus some of the things on the shelf I have never used.
Some are old & open, some old and never opened. All kinds of things, DMAE, DMP-30, BDMA, Spurr's components, Epon 812 substitutes, some Araldites, DBP, NMA, DDSA, Quetol, Maraglas, and Cardolite, LR White, the list goes on. All have been stored at RT in a glass doored cabinet in the lab, most are in brown glass bottles.
I'm ready to purge the lab of old, unused things, but don't want to discard anything that would be useful for the future. Happy to share a complete list and give away what we don't need.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 14, 34 -- From jkrupp-at-deltacollege.edu Thu May 5 11:13:35 2011 14, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 14, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p45GDZmM018397 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 11:13:35 -0500 14, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 14, 34 -- by localhost (Email Security Appliance) with SMTP id 060E795FCF6_DC2CCAFB 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 16:13:35 +0000 (GMT) 14, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 14, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id CFC8F95FCEB_DC2CCAEF 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 16:13:34 +0000 (GMT) 14, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 14, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 14, 34 -- ESMTP id LKQD2M00.39W for {Microscopy-at-microscopy.com} ; Thu, 5 14, 34 -- May 2011 09:13:34 -0700 14, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 14, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 98B1B7A3857C 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 09:13:34 -0700 (PDT) 14, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 14, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 14, 34 -- with ESMTP id zjkctGmyEj7E for {Microscopy-at-microscopy.com} ; 14, 34 -- Thu, 5 May 2011 09:13:34 -0700 (PDT) 14, 34 -- Received: from [172.20.3.238] (unknown [172.20.3.238]) 14, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id F3C917A3853F 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 09:13:33 -0700 (PDT) 14, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 14, 34 -- Content-Type: text/plain; charset=us-ascii 14, 34 -- Subject: Resin shelf life 14, 34 -- Date: Thu, 5 May 2011 09:13:33 -0700 14, 34 -- Message-Id: {3FC3F2E2-564A-4981-89DF-D5C627B10DF9-at-deltacollege.edu} 14, 34 -- To: Microscopy-at-microscopy.com 14, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 14, 34 -- X-Mailer: Apple Mail (2.1084) 14, 34 -- Content-Transfer-Encoding: 8bit 14, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45GDZmM018397 ==============================End of - Headers==============================
If you decide to discard some or all of them, let me tell you a story:
Roughly 20 years ago I worked in the clinical EM lab for our teaching hospital. As the budget got tight, my junior coworker cross-trained with the risk management people to give him some job security. One day he was helping the risk management people to clean out the "boom room", a negative pressure, continuous air-flow room where volatile solvents were stored. In a corner of this room were a number of bottles of EM resins (mostly LX-112 and Spurrs components). Now my coworker knew that the resins were hazardous, but he reasoned that they were less hazardous once they had polymerized. So, with good intentions, he mixed everything together in a 1 gal brown glass bottle.
Unfortunately, polymerization is an exothermic reaction and the mixture soon got hot and started to bubble. They quickly put the bottle in a secondary container (bucket) in case it overflowed. One problem, the hospital loading dock (where the boom room was located) was also the location for the air intake for several floors of the building [bad design choice]. If you have ever worked with DMP-30, it smells strongly like the inside of a bicycle tire. Pretty soon there were calls coming in that people in the building were getting nauseous, so they moved my coworker and his bubbling mess across the street. One small problem, he somehow managed to stand in the area where the air intake for that building was and again people started calling in complaining about the smell.
2-3hrs later they got everything under control and my coworker showed up back in the lab reeking of DMP-30. We sent him home and strongly encouraged him to take a shower and wash his clothes immediately.
In summary, I don't know the answer to your question (although if any of the components are hygroscopic, they would be suspect), but I encourage you to be careful in how you dispose of what you don't plan on keeping.
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ Douglas W. Cromey, M.S. - Assistant Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
http://swehsc.pharmacy.arizona.edu/exppath/ Home of: "Microscopy and Imaging Resources on the WWW"
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Thursday, May 05, 2011 9:14 AM To: Cromey, Douglas W - (dcromey)
Greetings
I think I may have asked this before. What are your ideas about the shelf life of embedding resins?
We have a bunch, some very old (a few back to 1983), and I don't know what to do with them. Are they good to use or should they just go?
I know I could mix up test sets, but I'm not sure I can spare the time. Plus some of the things on the shelf I have never used.
Some are old & open, some old and never opened. All kinds of things, DMAE, DMP-30, BDMA, Spurr's components, Epon 812 substitutes, some Araldites, DBP, NMA, DDSA, Quetol, Maraglas, and Cardolite, LR White, the list goes on. All have been stored at RT in a glass doored cabinet in the lab, most are in brown glass bottles.
I'm ready to purge the lab of old, unused things, but don't want to discard anything that would be useful for the future. Happy to share a complete list and give away what we don't need.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 14, 34 -- From jkrupp-at-deltacollege.edu Thu May 5 11:13:35 2011 14, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 14, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p45GDZmM018397 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 11:13:35 -0500 14, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 14, 34 -- by localhost (Email Security Appliance) with SMTP id 060E795FCF6_DC2CCAFB 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 16:13:35 +0000 (GMT) 14, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 14, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id CFC8F95FCEB_DC2CCAEF 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 16:13:34 +0000 (GMT) 14, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 14, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 14, 34 -- ESMTP id LKQD2M00.39W for {Microscopy-at-microscopy.com} ; Thu, 5 14, 34 -- May 2011 09:13:34 -0700 14, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 14, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 98B1B7A3857C 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 09:13:34 -0700 (PDT) 14, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 14, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 14, 34 -- with ESMTP id zjkctGmyEj7E for {Microscopy-at-microscopy.com} ; 14, 34 -- Thu, 5 May 2011 09:13:34 -0700 (PDT) 14, 34 -- Received: from [172.20.3.238] (unknown [172.20.3.238]) 14, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id F3C917A3853F 14, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 5 May 2011 09:13:33 -0700 (PDT) 14, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 14, 34 -- Content-Type: text/plain; charset=us-ascii 14, 34 -- Subject: Resin shelf life 14, 34 -- Date: Thu, 5 May 2011 09:13:33 -0700 14, 34 -- Message-Id: {3FC3F2E2-564A-4981-89DF-D5C627B10DF9-at-deltacollege.edu} 14, 34 -- To: Microscopy-at-microscopy.com 14, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 14, 34 -- X-Mailer: Apple Mail (2.1084) 14, 34 -- Content-Transfer-Encoding: 8bit 14, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45GDZmM018397 ==============================End of - Headers==============================
==============================Original Headers============================== 31, 47 -- From dcromey-at-email.arizona.edu Thu May 5 11:41:44 2011 31, 47 -- Received: from TX2EHSOBE001.bigfish.com (tx2ehsobe001.messaging.microsoft.com [65.55.88.11]) 31, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45Gfhm9002441 31, 47 -- for {Microscopy-at-Microscopy.Com} ; Thu, 5 May 2011 11:41:43 -0500 31, 47 -- Received: from mail150-tx2-R.bigfish.com (10.9.14.237) by 31, 47 -- TX2EHSOBE001.bigfish.com (10.9.40.21) with Microsoft SMTP Server id 31, 47 -- 14.1.225.8; Thu, 5 May 2011 16:41:43 +0000 31, 47 -- Received: from mail150-tx2 (localhost.localdomain [127.0.0.1]) by 31, 47 -- mail150-tx2-R.bigfish.com (Postfix) with ESMTP id 3EF4A3B834B; Thu, 5 May 31, 47 -- 2011 16:41:43 +0000 (UTC) 31, 47 -- X-SpamScore: -24 31, 47 -- X-BigFish: VS-24(zz9371O62a3K7f0R542Mzz1202hzz8275bhz32i87h2a8h668h839h61h) 31, 47 -- X-Spam-TCS-SCL: 0:0 31, 47 -- X-Forefront-Antispam-Report: KIP:(null);UIP:(null);IPVD:NLI;H:va3diahub023.RED001.local;RD:smtp801.microsoftonline.com;EFVD:NLI 31, 47 -- X-FB-DOMAIN-IP-MATCH: fail 31, 47 -- Received: from mail150-tx2 (localhost.localdomain [127.0.0.1]) by mail150-tx2 31, 47 -- (MessageSwitch) id 1304613550158385_29725; Thu, 5 May 2011 16:39:10 +0000 31, 47 -- (UTC) 31, 47 -- Received: from TX2EHSMHS038.bigfish.com (unknown [10.9.14.237]) by 31, 47 -- mail150-tx2.bigfish.com (Postfix) with ESMTP id AFC195100A5; Thu, 5 May 2011 31, 47 -- 16:37:18 +0000 (UTC) 31, 47 -- Received: from va3diahub023.RED001.local (65.55.171.153) by 31, 47 -- TX2EHSMHS038.bigfish.com (10.9.99.138) with Microsoft SMTP Server (TLS) id 31, 47 -- 14.1.225.8; Thu, 5 May 2011 16:37:17 +0000 31, 47 -- Received: from VA3DIAXVS211.RED001.local ([10.32.56.196]) by 31, 47 -- va3diahub023.RED001.local ([10.32.21.23]) with mapi; Thu, 5 May 2011 09:36:53 31, 47 -- -0700 31, 47 -- From: "Cromey, Douglas W - (dcromey)" {dcromey-at-email.arizona.edu} 31, 47 -- To: "jkrupp-at-deltacollege.edu" {jkrupp-at-deltacollege.edu} 31, 47 -- CC: "Microscopy listserv (Microscopy-at-Microscopy.Com)" 31, 47 -- {Microscopy-at-Microscopy.Com} 31, 47 -- Date: Thu, 5 May 2011 09:36:49 -0700 31, 47 -- Subject: RE: [Microscopy] Resin shelf life 31, 47 -- Thread-Topic: [Microscopy] Resin shelf life 31, 47 -- Thread-Index: AcwLP3tdYdk49TNUSk+nJJ4LZH5UQgAAIW0Q 31, 47 -- Message-ID: {33EA1C13F421004C924AD94E7BF09A7D055E77CB28-at-VA3DIAXVS211.RED001.local} 31, 47 -- References: {201105051614.p45GE7mS019127-at-ns.microscopy.com} 31, 47 -- In-Reply-To: {201105051614.p45GE7mS019127-at-ns.microscopy.com} 31, 47 -- Accept-Language: en-US 31, 47 -- Content-Language: en-US 31, 47 -- X-MS-Has-Attach: 31, 47 -- X-MS-TNEF-Correlator: 31, 47 -- acceptlanguage: en-US 31, 47 -- Content-Type: text/plain; charset="us-ascii" 31, 47 -- MIME-Version: 1.0 31, 47 -- Content-Transfer-Encoding: 8bit 31, 47 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p45Gfhm9002441 ==============================End of - Headers==============================
In a slightly peripheral but related question, I was wondering about the shelf life of Permount. We have a very old bottle in our lab, and it's been opened some time in the past. We rarely do alcohol/xylene series embedding, in fact, never have during the time I've been there. But if we were to, would an old bottle of Permount still be usable, or would the xylene base evaporated off too greatly to be usable?
Peter
On May 5, 2011, at 9:22 AM, jkrupp-at-deltacollege.edu wrote:
} Greetings } } I think I may have asked this before. What are your ideas about the } shelf life of embedding resins?
==============================Original Headers============================== 6, 21 -- From germpore-at-sonic.net Thu May 5 11:59:48 2011 6, 21 -- Received: from qmta13.emeryville.ca.mail.comcast.net (qmta13.emeryville.ca.mail.comcast.net [76.96.27.243]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45GxlcL018868 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2011 11:59:48 -0500 6, 21 -- Received: from omta22.emeryville.ca.mail.comcast.net ([76.96.30.89]) 6, 21 -- by qmta13.emeryville.ca.mail.comcast.net with comcast 6, 21 -- id fsSF1g00D1vN32cADsznGk; Thu, 05 May 2011 16:59:47 +0000 6, 21 -- Received: from [192.168.2.15] ([24.5.115.217]) 6, 21 -- by omta22.emeryville.ca.mail.comcast.net with comcast 6, 21 -- id fszm1g00G4hVnAG8iszmvY; Thu, 05 May 2011 16:59:46 +0000 6, 21 -- Message-Id: {84378507-EA5A-4BC2-802E-812A0E050FCA-at-sonic.net} 6, 21 -- From: Peter Werner {germpore-at-sonic.net} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- In-Reply-To: {201105051622.p45GMPZn000324-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Permount shelf life 6, 21 -- Date: Thu, 5 May 2011 09:59:45 -0700 6, 21 -- References: {201105051622.p45GMPZn000324-at-ns.microscopy.com} 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Thank You to those who responded to my question about putting Bakelite mounts into a UHV chamber. Your comments were very helpful. I think the mounts would have been fine, but they were too big to fit into our chamber, so the samples had to be extricated from the mounts. Hammer + Undergraduate = Success. - Jen
==============================Original Headers============================== 2, 35 -- From jensloppy-at-gmail.com Thu May 5 12:47:57 2011 2, 35 -- Received: from mail-ew0-f41.google.com (mail-ew0-f41.google.com [209.85.215.41]) 2, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45Hlv5Y003197 2, 35 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2011 12:47:57 -0500 2, 35 -- Received: by ewy9 with SMTP id 9so831982ewy.0 2, 35 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2011 10:47:56 -0700 (PDT) 2, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 35 -- d=gmail.com; s=gamma; 2, 35 -- h=domainkey-signature:mime-version:sender:date:x-google-sender-auth 2, 35 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 2, 35 -- bh=18rB2vrvz+Wt+CnUVRyu06Hx/lop0TtKUoTH1nZg+gA=; 2, 35 -- b=QNQEPcQMBaEeyddm1E4J7M9QWv7WkyXGh/BFZppAwfgbQ8fFhpMx5BBcFyugfgCT8f 2, 35 -- El4AWm7I3Dgqry15Px6Pbhxq3FXS7J/qQgbuEWuo50Ujcne8r4o2h2QZT2sfV72sqwLI 2, 35 -- Y29iCjBbidL75wowTB2J+U+PecCZVUiQ5gqQ0= 2, 35 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 35 -- d=gmail.com; s=gamma; 2, 35 -- h=mime-version:sender:date:x-google-sender-auth:message-id:subject 2, 35 -- :from:to:content-type:content-transfer-encoding; 2, 35 -- b=UKCyzcmGaWJrzrt+gOOl/3GyIPKSZLCZmdomiVKYt/yJFKFK19qyaQQxwc+PcQLKow 2, 35 -- B1CMtlOc+o7G+cgxW8JOT9TPfql1+VQSV3AglBIfu/0pOYP6nYtvkRlb2Zk7S2fg5+VI 2, 35 -- pKnku1kjXyh7+Bp/9Rp0rCrrBUJsbyFUVnFPc= 2, 35 -- MIME-Version: 1.0 2, 35 -- Received: by 10.14.42.200 with SMTP id j48mr1285805eeb.206.1304617676820; Thu, 2, 35 -- 05 May 2011 10:47:56 -0700 (PDT) 2, 35 -- Sender: jensloppy-at-gmail.com 2, 35 -- Received: by 10.14.53.73 with HTTP; Thu, 5 May 2011 10:47:56 -0700 (PDT) 2, 35 -- Date: Thu, 5 May 2011 13:47:56 -0400 2, 35 -- X-Google-Sender-Auth: 1S2EnrfMKT9CuLGNUzIpK5tviK4 2, 35 -- Message-ID: {BANLkTi=MhaXcwxcu-tiC9i15wH8J_3gmHw-at-mail.gmail.com} 2, 35 -- Subject: Thanks for Bakelite in UHV responses 2, 35 -- From: Jen Sloppy {jds451-at-psu.edu} 2, 35 -- To: microscopy {microscopy-at-microscopy.com} 2, 35 -- Content-Type: text/plain; charset=ISO-8859-1 2, 35 -- Content-Transfer-Encoding: 8bit 2, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45Hlv5Y003197 ==============================End of - Headers==============================
Having attempted to use an old bottle of Permount, I'd suggest that you dispose of it now and get a new bottle when the time comes to coverslip again.
IF you can get the cap off you'd be lucky and the fluid is most likely at least partially dehydrated and thick to the point that it will not flow properly under the coverslip.
Pat Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
In a slightly peripheral but related question, I was wondering about the shelf life of Permount. We have a very old bottle in our lab, and it's been opened some time in the past. We rarely do alcohol/xylene series embedding, in fact, never have during the time I've been there. But if we were to, would an old bottle of Permount still be usable, or would the xylene base evaporated off too greatly to be usable?
Peter
==============================Original Headers============================== 10, 28 -- From connellyps-at-nhlbi.nih.gov Thu May 5 13:01:46 2011 10, 28 -- Received: from nihxwayout.hub.nih.gov (nihxwayout.hub.nih.gov [128.231.90.109]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45I1kse019317 10, 28 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2011 13:01:46 -0500 10, 28 -- X-IronPortListener: Outbound_SMTP 10, 28 -- X-IronPort-Anti-Spam-Filtered: true 10, 28 -- X-IronPort-Anti-Spam-Result: AvsEAI7kwk2cKEcV/2dsb2JhbACmPXemBYNxm2gCgyKCYwSPSoRAiis 10, 28 -- Received: from unknown (HELO NIHHT02.nih.gov) ([156.40.71.21]) 10, 28 -- by nihxwayout.hub.nih.gov with ESMTP; 05 May 2011 14:01:44 -0400 10, 28 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT02.nih.gov 10, 28 -- ([156.40.71.21]) with mapi; Thu, 5 May 2011 14:01:41 -0400 10, 28 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 10, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 28 -- Date: Thu, 5 May 2011 14:01:41 -0400 10, 28 -- Subject: Re: [Microscopy] Permount shelf life 10, 28 -- Thread-Topic: [Microscopy] Permount shelf life 10, 28 -- Thread-Index: AcwLRlfOQ2unCTzUTdWEQsPJ+VhCHwACCSjV 10, 28 -- Message-ID: {C9E85E45.7F72%connellyps-at-nhlbi.nih.gov} 10, 28 -- In-Reply-To: {201105051703.p45H3Ntx025385-at-ns.microscopy.com} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="iso-8859-1" 10, 28 -- MIME-Version: 1.0 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45I1kse019317 ==============================End of - Headers==============================
The DMP-30 has a very short shelf life and the LR White should have been in the refrigerator. Any old open bottles of NMA and DDSA and anything else that is supposed to be anhydrous need to be discarded. Unopened bottles of Epon 812 substitutes should be fine for years.
My best suggestion would be to make a list with the lot numbers and send it to the suppliers and ask their opinions. Saving all the things in the world is not worth a failed embedding of an important experiment.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
I think I may have asked this before. What are your ideas about the shelf life of embedding resins?
We have a bunch, some very old (a few back to 1983), and I don't know what to do with them. Are they good to use or should they just go?
I know I could mix up test sets, but I'm not sure I can spare the time. Plus some of the things on the shelf I have never used.
Some are old & open, some old and never opened. All kinds of things, DMAE, DMP-30, BDMA, Spurr's components, Epon 812 substitutes, some Araldites, DBP, NMA, DDSA, Quetol, Maraglas, and Cardolite, LR White, the list goes on. All have been stored at RT in a glass doored cabinet in the lab, most are in brown glass bottles.
I'm ready to purge the lab of old, unused things, but don't want to discard anything that would be useful for the future. Happy to share a complete list and give away what we don't need.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 21, 28 -- From connellyps-at-nhlbi.nih.gov Thu May 5 13:13:51 2011 21, 28 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 21, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45IDpau003152 21, 28 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2011 13:13:51 -0500 21, 28 -- X-IronPortListener: Outbound_SMTP 21, 28 -- X-IronPort-Anti-Spam-Filtered: true 21, 28 -- X-IronPort-Anti-Spam-Result: AvsEABXowk2cKEcV/2dsb2JhbACmPXemAINxm2wCgyKCYwSPSoRAiis 21, 28 -- Received: from unknown (HELO NIHHT02.nih.gov) ([156.40.71.21]) 21, 28 -- by nihxway4out.hub.nih.gov with ESMTP; 05 May 2011 14:13:48 -0400 21, 28 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT02.nih.gov 21, 28 -- ([156.40.71.21]) with mapi; Thu, 5 May 2011 14:13:25 -0400 21, 28 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 21, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 21, 28 -- Date: Thu, 5 May 2011 14:13:25 -0400 21, 28 -- Subject: Re: [Microscopy] Resin shelf life 21, 28 -- Thread-Topic: [Microscopy] Resin shelf life 21, 28 -- Thread-Index: AcwLP/Xsgl70FlU5RiWSmr8p9Vs9MwAECoji 21, 28 -- Message-ID: {C9E86105.7F83%connellyps-at-nhlbi.nih.gov} 21, 28 -- In-Reply-To: {201105051617.p45GHRmf024890-at-ns.microscopy.com} 21, 28 -- Accept-Language: en-US 21, 28 -- Content-Language: en 21, 28 -- X-MS-Has-Attach: 21, 28 -- X-MS-TNEF-Correlator: 21, 28 -- acceptlanguage: en-US 21, 28 -- Content-Type: text/plain; charset="iso-8859-1" 21, 28 -- MIME-Version: 1.0 21, 28 -- Content-Transfer-Encoding: 8bit 21, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45IDpau003152 ==============================End of - Headers==============================
If memory serves, toluene is the normal Permount solvent - which is not to be used lightly to mount coverslips on xylene soaked sections. Specifically, I have almost Canada Balsam (Leitz, circa 1916) that is very viscous but still crystal clear. I would never throw such material away, nor would I fail to donate the near liter of Dammar in Xylene that I prepared almost 25 years ago. These latter materials are too precious to merely discard. Thus, for any who are seriously interested, please ask, and ye shall receive a fair aliquot, at least of the latter.
Permount? I never used the stuff, but then, I never did histology in bulk. My max was ~400 specimens for each of about 5 years during the middle 1990's, and all sections were covered manually, including those on the 2 x 3" slides. Ehrlichs Acid Hematoxylin, Gomori's Aldehyde Fuchsin (striking magenta for elastin), and Eosin (HG&E). Boy, how I loved the solitude during that part of my career/life! It was just me, my Leitz, and hundreds of slides. [Very, very strange, I admit!] Now, when I look into the binoculars and turn on the illuminator, I get to see a section with 10's of floaters in the foreground.
Finally, like other mountants, for as many times that I have seen a bottle of Permount on a shelf over the years (half empty), the fluid appears to be clear and of useful viscosity. Again, however, I have never been tempted.
Cheers,
Fred Monson
P.S. I also have histologic museum pieces: An unused, Fisher, "Tissuemat Knife" (paraffin knife), 120VAC, with original standard cord, instructions and almost complete box. AND A home-made but carefully designed and machined (anonymous) razor blade holder (4.75") for paraffin sectioning with big wing nuts to snug the blade clamp - 1" section width limit.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state. 1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008. 2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224
-----Original Message----- X-from: germpore-at-sonic.net [mailto:germpore-at-sonic.net] Sent: Thursday, May 05, 2011 1:07 PM To: Monson, Frederick
In a slightly peripheral but related question, I was wondering about the shelf life of Permount. We have a very old bottle in our lab, and it's been opened some time in the past. We rarely do alcohol/xylene series embedding, in fact, never have during the time I've been there. But if we were to, would an old bottle of Permount still be usable, or would the xylene base evaporated off too greatly to be usable?
Peter
On May 5, 2011, at 9:22 AM, jkrupp-at-deltacollege.edu wrote:
} Greetings } } I think I may have asked this before. What are your ideas about the } shelf life of embedding resins?
==============================Original Headers============================== 6, 21 -- From germpore-at-sonic.net Thu May 5 11:59:48 2011 6, 21 -- Received: from qmta13.emeryville.ca.mail.comcast.net (qmta13.emeryville.ca.mail.comcast.net [76.96.27.243]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45GxlcL018868 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2011 11:59:48 -0500 6, 21 -- Received: from omta22.emeryville.ca.mail.comcast.net ([76.96.30.89]) 6, 21 -- by qmta13.emeryville.ca.mail.comcast.net with comcast 6, 21 -- id fsSF1g00D1vN32cADsznGk; Thu, 05 May 2011 16:59:47 +0000 6, 21 -- Received: from [192.168.2.15] ([24.5.115.217]) 6, 21 -- by omta22.emeryville.ca.mail.comcast.net with comcast 6, 21 -- id fszm1g00G4hVnAG8iszmvY; Thu, 05 May 2011 16:59:46 +0000 6, 21 -- Message-Id: {84378507-EA5A-4BC2-802E-812A0E050FCA-at-sonic.net} 6, 21 -- From: Peter Werner {germpore-at-sonic.net} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- In-Reply-To: {201105051622.p45GMPZn000324-at-ns.microscopy.com} 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Permount shelf life 6, 21 -- Date: Thu, 5 May 2011 09:59:45 -0700 6, 21 -- References: {201105051622.p45GMPZn000324-at-ns.microscopy.com} 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
==============================Original Headers============================== 22, 36 -- From FMonson-at-wcupa.edu Thu May 5 15:24:27 2011 22, 36 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 22, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p45KOR02021706 22, 36 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2011 15:24:27 -0500 22, 36 -- X-ASG-Debug-ID: 1304627066-0378c321951adbf0001-4CH8be 22, 36 -- Received: from WCU-XCH-02.PASSHE.LCL (wcu-xch-02.wcupa.edu [144.26.0.86]) by MX02.WCUPA.EDU with ESMTP id jp2NbFelIehE1BUz (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 05 May 2011 16:24:26 -0400 (EDT) 22, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 22, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.86 22, 36 -- X-ASG-Whitelist: Client 22, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 22, 36 -- WCU-XCH-02.PASSHE.LCL ([fe80::7c95:2a81:a8ef:432b%16]) with mapi id 22, 36 -- 14.01.0270.001; Thu, 5 May 2011 16:24:26 -0400 22, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 22, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 22, 36 -- Subject: RE: [Microscopy] Permount shelf life 22, 36 -- Thread-Topic: [Microscopy] Permount shelf life 22, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] Permount shelf life 22, 36 -- Thread-Index: AQHMC0bI2PH+gUnQDUWWFpQ0+1tOn5R+ejGQ 22, 36 -- Date: Thu, 5 May 2011 20:24:25 +0000 22, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C05CF27-at-WCU-XCH-03.PASSHE.LCL} 22, 36 -- References: {201105051706.p45H6VG0031494-at-ns.microscopy.com} 22, 36 -- In-Reply-To: {201105051706.p45H6VG0031494-at-ns.microscopy.com} 22, 36 -- Accept-Language: en-US 22, 36 -- Content-Language: en-US 22, 36 -- X-MS-Has-Attach: 22, 36 -- X-MS-TNEF-Correlator: 22, 36 -- x-originating-ip: [10.28.57.79] 22, 36 -- Content-Type: text/plain; charset="us-ascii" 22, 36 -- MIME-Version: 1.0 22, 36 -- X-Barracuda-Connect: wcu-xch-02.wcupa.edu[144.26.0.86] 22, 36 -- X-Barracuda-Start-Time: 1304627066 22, 36 -- X-Barracuda-Encrypted: AES128-SHA 22, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 22, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 22, 36 -- Content-Transfer-Encoding: 8bit 22, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p45KOR02021706 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cvierret-at-mst.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: MS&T
Title-Subject: [Filtered] Stigmation Issue
Message: We have a Hitachi S4700. Last year it started to have stigmation issues. The left stigmation would peg out and we were not able to focus past 5000x. This was on every sample, in fact magnetic samples tend to do a bit better with stigmation. The problem gets worse the longer we use the scope. Todate the service persons have rebuilt the column, installed a new source, replace the objective lens (or the bottom unit if you don't want to consider it an objective lens). We are waiting on the bottom cover of the pole piece, this might take some time considering it will be coming from Japan. I was wondering if anybody else has had this issue and if so was it every corrected.
Thanking you for your replies in advance,
Clarissa Wisner Electron Microscope Specialist MS&T
Clarissa, You didn't mention whether or not the engineers have looked at the signal going to the stigmator coils. If they haven't, have them do that. In my experience on other brands if there is noise on the signal at under 500kHz with an amplitude over about 10mV, you can have uncorrectable astigmatism.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, May 05, 2011 7:40 PM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cvierret-at-mst.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: MS&T
Title-Subject: [Filtered] Stigmation Issue
Message: We have a Hitachi S4700. Last year it started to have stigmation issues. The left stigmation would peg out and we were not able to focus past 5000x. This was on every sample, in fact magnetic samples tend to do a bit better with stigmation. The problem gets worse the longer we use the scope. Todate the service persons have rebuilt the column, installed a new source, replace the objective lens (or the bottom unit if you don't want to consider it an objective lens). We are waiting on the bottom cover of the pole piece, this might take some time considering it will be coming from Japan. I was wondering if anybody else has had this issue and if so was it every corrected.
Thanking you for your replies in advance,
Clarissa Wisner Electron Microscope Specialist MS&T
We recently went through a similar purchasing decision for SEM where we did some detailed tests on EDS systems. The first thing to note, is they were all good. I'm not aware of any bad EDS systems. However, Oxford's X-Max pleasantly surprised me. I will describe one of the tests that you may find useful:
I gave them a chromite microprobe standard that was embedded in epoxy and microtomed so the surface was macroscopically flat, but not polished (roughness on the micron scale). Of course, I did not tell them it was chromite. I then asked for a standardless quant. All elements were identified by auto-ID except silicon (microprobe reported abundance of Si at 0.005 Wt%). The successful auto-ID included V (microprobe=0.122 Wt%) despite the fact that the V peak was interfering with Ti Kb. It also successfully found Mn (microprobe=0.225 Wt%) despite the fact that Mn Ka was buried under a massive Cr Kb peak (microprobe Cr = 31.905 Wt%!!!)
That was at 100,000 cps, on an unpolished surface! I was present the entire time. The whole operation was done in a few minutes -- the average of several spectra. The only catch is that they didn't catch the V or Mn at first because they were doing 10 second spectra. (I suppose they were trying to impress me with their speed?) I told them to sit on it for longer until it had enough counts and the auto-ID found the V and Mn. That was the only "hint" I gave them.
Having used mostly their older detectors Si(Li) on TEM and SEM, I find they are obviously not as good as the new ones, but still very reliable. I will often use an inferior e-beam instrument because it has the Oxford detector when I'm doing EDS because the results come out so nice.
I also share some disappointment with the Inca software -- it doesn't let you get under the hood of the computations. It also has some unpleasant quirks. For example, trying to do standards based quant in a TEM can be fussy because you only have one set of k-factors. I see the logic in this, but in a multiuser environment, that's not always a good thing.
I have found the hardware to be robust. One EDS I know of is poorly treated by grad students (room temp, cryogenic, room temp, cryo...) It still works....
I hope this helps and I didn't rattle your ears off.
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA 94720 510-642-9733 zackg-at-ssl.berkeley.edu
On May 3, 2011, at 9:27 PM, microscopylistserver-noreply-at-microscopy.com wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both y.han.1-at-bham.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: y.han.1-at-bham.ac.uk Name: Yisong Han
Organization: University of Birmingham
Title-Subject: [Filtered] EDX
Message: Dear All,
We planned to buy an SDD EDX for our JEOL 2100f probe corrected TEM with UHR pole pieces. We can go with either an Oxford X-MAX or a Bruker. I am wondering if anyone could comment on these two systems in terms of ease of use, reliability, sensitivity, compatibility with JEOL machines, etc. Thanks very much in advance.
I've never experienced this problem on my SEM and don't recall ever seeing any posts associating it with Hitachi SEM's. I've used an S-4700 for the past 9 years so I pay pretty close attention to posts associated with Hitachi. I recently had a stigmation issue with mine but it was related to sample material migrating to the Bse detector. A quick cleaning and we were back on line.
John A. Robson Research Scientist Boehringer Ingelheim Pharmaceuticals, Inc. (203)798-5640
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, May 05, 2011 7:49 PM To: Robson,John (AN) BIP-US-R
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cvierret-at-mst.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: MS&T
Title-Subject: [Filtered] Stigmation Issue
Message: We have a Hitachi S4700. Last year it started to have stigmation issues. The left stigmation would peg out and we were not able to focus past 5000x. This was on every sample, in fact magnetic samples tend to do a bit better with stigmation. The problem gets worse the longer we use the scope. Todate the service persons have rebuilt the column, installed a new source, replace the objective lens (or the bottom unit if you don't want to consider it an objective lens). We are waiting on the bottom cover of the pole piece, this might take some time considering it will be coming from Japan. I was wondering if anybody else has had this issue and if so was it every corrected.
Thanking you for your replies in advance,
Clarissa Wisner Electron Microscope Specialist MS&T
Microscopy Core, at Office of Collaborative Science of New York University Langone Medical Center, provides state-of-the-art imaging and visualization resources for the School of Medicine and local scientific community. Currently, we¹re seeking a full time research technician with expertise in current techniques for electron microscopy.
In this position you will coordinate operations of electron microscopes in the Microscopy core, assist with sample preparation, proficiency in cutting ultrathin sections and train users in microscope. Computer skills and research experience are a plus. The ideal applicant will have basic knowledge of transmission electron microscope, a BS/MS in biology or a related scientific field with 2-5 years¹ electron microscopy experience. The individual should be capable of multitasking and should enjoy working with other people. Good communication skills are essential. Qualified applicants should send a curriculum vitae and names of three references to Alice Liang (Fengxia.Liang-at-med.nyu.edu). Salary is commensurate with experience. The position is currently open and applications will be reviewed continuously until the position is filled. (http://cores.webdev.med.nyu.edu/list-cores/microscopy-imaging)
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 5, 30 -- From Fengxia.Liang-at-med.nyu.edu Fri May 6 09:06:43 2011 5, 30 -- Received: from mail4.nyumc.org (mail4.nyumc.org [216.165.126.170]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p46E6hb0010246 5, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 6 May 2011 09:06:43 -0500 5, 30 -- X-IronPort-Anti-Spam-Filtered: true 5, 30 -- X-IronPort-Anti-Spam-Result: ApsEAAH/w00KkyXl/2dsb2JhbACnOaY1ljyIb4YJBJ5I 5, 30 -- X-IronPort-AV: E=Sophos;i="4.64,326,1301889600"; 5, 30 -- d="scan'208";a="131886644" 5, 30 -- Received: from msgwsdcpht63.nyumc.org ([10.147.37.229]) 5, 30 -- by mail4.nyumc.org with SMTP; 06 May 2011 10:06:43 -0400 5, 30 -- Received: from MSGWSDCPMB05.nyumc.org ([10.147.54.52]) by 5, 30 -- MSGWSDCPHT63.nyumc.org ([10.147.37.229]) with mapi; Fri, 6 May 2011 10:06:43 5, 30 -- -0400 5, 30 -- From: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 5, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 30 -- Date: Fri, 6 May 2011 10:06:41 -0400 5, 30 -- Subject: Electron microscopy technician position available at NYULMC 5, 30 -- Thread-Topic: Electron microscopy technician position available at NYULMC 5, 30 -- Thread-Index: AcwL9tKY5R63+N6Cu0Wpr4vqk+3D7Q== 5, 30 -- Message-ID: {C9E978B1.7969%Fengxia.Liang-at-med.nyu.edu} 5, 30 -- Accept-Language: en-US 5, 30 -- Content-Language: en-US 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- user-agent: Microsoft-Entourage/13.4.0.100208 5, 30 -- acceptlanguage: en-US 5, 30 -- Content-Type: text/plain; charset="iso-8859-1" 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p46E6hb0010246 ==============================End of - Headers==============================
The Analytical Imaging Facility of the Albert Einstein College of Medicine has an opening for a research technician with skills in biological electron microscopy.
Description of the facility can be found here: http://www.einstein.yu.edu/aif/page.aspx
Details regarding the position are here: https://einsteincareers-yeshiva.icims.com/jobs/1460/job
Frank Macaluso Administrative Director and Director of Electron Microscopy Analytical Imaging Facility Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx, NY 10461
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Organization: LBNL
Title-Subject: [Filtered] Clamshell Cu TEM grids
Message: Who sells Cu clamshell TEM grids? Login Host: 198.128.31.46 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue May 10 07:27:17 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4ACRHt9032446 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2011 07:27:17 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 9B36A395255 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2011 07:27:17 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id zgCiURTnw+Yq for {microscopy-at-microscopy.com} ; 7, 25 -- Tue, 10 May 2011 07:27:16 -0500 (CDT) 7, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id EA2D339524A 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2011 07:27:16 -0500 (CDT) 7, 25 -- Message-ID: {4DC92F24.9090300-at-microscopy.com} 7, 25 -- Date: Tue, 10 May 2011 07:27:16 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: mlibbee-at-gmail.com 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Clamshell Cu TEM grids 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
All the EM Suppliers have these grids available so if you already have a favorite supplier check their web catalogue. The name may not be "Clam Shell Grids" but "Double Folding Grids" depending on the supplier.
If you do not have a current supplier just Google "Electron Microscopy Supplies" and many companies will be listed with their web sites. After choosing a company put the name (above) into the search box and you should have your answer. I have checked two catalogues that I have in my lab for the name of the grids for I have not bought any for quite some time.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
===== This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Organization: LBNL
Title-Subject: [Filtered] Clamshell Cu TEM grids
Message: Who sells Cu clamshell TEM grids? Login Host: 198.128.31.46
==============================Original Headers============================== 13, 29 -- From connellyps-at-nhlbi.nih.gov Tue May 10 09:35:48 2011 13, 29 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 13, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4AEZlID018051 13, 29 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2011 09:35:48 -0500 13, 29 -- X-IronPortListener: Outbound_SMTP 13, 29 -- X-IronPort-Anti-Spam-Filtered: true 13, 29 -- X-IronPort-Anti-Spam-Result: AmgIAFBHyU2cKEcW/2dsb2JhbACbFoQDhmV3qjKbfwKDJAEBgmcEh1yIFIREg3yGOg 13, 29 -- Received: from unknown (HELO NIHHT03.nih.gov) ([156.40.71.22]) 13, 29 -- by nihxway4out.hub.nih.gov with ESMTP; 10 May 2011 10:35:47 -0400 13, 29 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT03.nih.gov 13, 29 -- ([156.40.71.22]) with mapi; Tue, 10 May 2011 10:35:47 -0400 13, 29 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 13, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 13, 29 -- "mlibbee-at-gmail.com" {mlibbee-at-gmail.com} 13, 29 -- Date: Tue, 10 May 2011 10:35:46 -0400 13, 29 -- Subject: Re: [Microscopy] via WWW:Clamshell Cu TEM grids 13, 29 -- Thread-Topic: [Microscopy] via WWW:Clamshell Cu TEM grids 13, 29 -- Thread-Index: AcwPDihnj4mBn5iZQimOrPgRb39A3wAEWPyY 13, 29 -- Message-ID: {C9EEC582.7FE5%connellyps-at-nhlbi.nih.gov} 13, 29 -- In-Reply-To: {201105101231.p4ACVGi8006511-at-ns.microscopy.com} 13, 29 -- Accept-Language: en-US 13, 29 -- Content-Language: en 13, 29 -- X-MS-Has-Attach: 13, 29 -- X-MS-TNEF-Correlator: 13, 29 -- acceptlanguage: en-US 13, 29 -- Content-Type: text/plain; charset="iso-8859-1" 13, 29 -- MIME-Version: 1.0 13, 29 -- Content-Transfer-Encoding: 8bit 13, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4AEZlID018051 ==============================End of - Headers==============================
Microscopy Core, at Office of Collaborative Science of New York University Langone Medical Center, provides state-of-the-art imaging and visualization resources for the School of Medicine and local scientific community. Currently, we¹re seeking a full time research technician with expertise in current techniques for electron microscopy.
In this position you will coordinate operations of electron microscopes in the Microscopy core, assist with sample preparation, proficiency in cutting ultrathin sections and train users in microscope. Computer skills and research experience are a plus. The ideal applicant will have basic knowledge of transmission electron microscope, a BS/MS in biology with 2-5 years¹ electron microscopy experience, or associated degree in Electron Microscopy with basic biology knowledge. The individual should be capable of multitasking and should enjoy working with other people. Good communication skills are essential. Qualified applicants should send a curriculum vitae and names of three references to Alice Liang (Fengxia.Liang-at-med.nyu.edu). Salary is commensurate with experience. The position is currently open and applications will be reviewed continuously until the position is filled. (http://cores.webdev.med.nyu.edu/list-cores/microscopy-imaging)
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 5, 30 -- From Fengxia.Liang-at-med.nyu.edu Tue May 10 10:35:21 2011 5, 30 -- Received: from mail3.nyumc.org (mail3.nyumc.org [216.165.126.230]) 5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4AFZLxb002593 5, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2011 10:35:21 -0500 5, 30 -- X-IronPort-Anti-Spam-Filtered: true 5, 30 -- X-IronPort-Anti-Spam-Result: ApsEANJZyU0KkyXk/2dsb2JhbACnAKculnqIcIYPBJ5q 5, 30 -- X-IronPort-AV: E=Sophos;i="4.64,346,1301889600"; 5, 30 -- d="scan'208";a="139651503" 5, 30 -- Received: from msgwsdcpht62.nyumc.org ([10.147.37.228]) 5, 30 -- by mail3.nyumc.org with SMTP; 10 May 2011 11:35:19 -0400 5, 30 -- Received: from MSGWSDCPMB05.nyumc.org ([10.147.54.52]) by 5, 30 -- MSGWSDCPHT62.nyumc.org ([10.147.37.228]) with mapi; Tue, 10 May 2011 11:35:19 5, 30 -- -0400 5, 30 -- From: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 5, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 30 -- Date: Tue, 10 May 2011 11:35:15 -0400 5, 30 -- Subject: Electron microscopy technician position available at NYULMC 5, 30 -- Thread-Topic: Electron microscopy technician position available at NYULMC 5, 30 -- Thread-Index: AcwPJ9ujT1ma2IdI0Uu4xssntXA5qQ== 5, 30 -- Message-ID: {C9EED373.7A27%Fengxia.Liang-at-med.nyu.edu} 5, 30 -- Accept-Language: en-US 5, 30 -- Content-Language: en-US 5, 30 -- X-MS-Has-Attach: 5, 30 -- X-MS-TNEF-Correlator: 5, 30 -- user-agent: Microsoft-Entourage/13.4.0.100208 5, 30 -- acceptlanguage: en-US 5, 30 -- Content-Type: text/plain; charset="iso-8859-1" 5, 30 -- MIME-Version: 1.0 5, 30 -- Content-Transfer-Encoding: 8bit 5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4AFZLxb002593 ==============================End of - Headers==============================
I would avoid osmium as it likely would oxidize your antigen. We high pressure freeze arabidopsis anthers dissected out of the flowers (can freeze 6-10 per planchette) and sub them with the top hat removed. Preventing extraction of lipids is a challenge but you might be able to do this by warming only to minus 50 C after freeze substitution, and embedding in something like Lowicryl HM20 at this temperature. I have had success in immunoreactivity using this approach and subbing only in acetone.
For general advice on preserving lipids for TEM, the expert is Lacey Samuels at UBC, Vancouver. She's also done quite a bit of immuno-EM.
Or your colleague could look up:
Mamun EA, Cantrill LC, Overall RL, Sutton BG 2005 Cellular organisation in meiotic and early post-meiotic rice anthers. Cell Biology International 29: 903-913
or here's an unpublished protocol from a thesis - really spectacular images of pollen at various stages of development in rice anthers:
The cryofixation protocol for immunolabelling was modified from Paxson-Sowders et al. (2001). Anthers at the required stages were cut and placed in specimen holders pre-coated with 7.5% (w/v) lecithin in chloroform and filled with 0.2M sucrose. Then samples were frozen in a Leica EM PACT Freezer (Leica, Germany). Following freezing, samples were freeze-substituted in 3% paraformaldehyde and 0.5% uranyl acetate in anhydrous acetone at -90ºC for 60 hours, -60ºC for 12 hours, -30ºC for 6 hours and then brought up to 20ºC with a temperature increase rate of +5ºC per hour in a Leica EM AFS (automatic freeze substitution) machine (Leica, Germany). Following this the anthers were rinsed 3 times in anhydrous acetone and infiltrated in a graded acetone/LR gold acrylic resin mixture and 100% LR gold resin for 5 days with two changes of fresh resin per day and polymerised under UV at -20ºC in the Leica EM AFS for 24 hours.
Immunolabelling techniques were modified from methods for indirect immunogold labelling of ultra-thin sections for TEM (Cole, 1996) and immunofluorescence labelling (Harper et al., 1996). One micrometer sections of LR-Gold embedded blocks were placed on poly-L-lysine coated slides and were blocked with 200 μl per sample of blocking buffer containing phosphate buffered saline (PBS) (0.8% NaCl, 0.02% KCl, 0.115% Na2HPO4, KH2PO4 and 0.02% NaN3, pH6.9) and 1% BSA at room temperature for 1 hour. Then the sections were incubated in a 1:100 solution of primary antibodies for callose and callose synthase in blocking buffer at 4ºC overnight at a rate of 20μl per sample. Sections were washed 3 times with PBS for 5 minutes each, followed by incubation of secondary antibody (1:50 dilution with the same blocking buffer) at 37 ºC for 3 hours. Sections were washed again 3 times with PBS for 5 minutes each, followed by background staining with propidium iodide (10μg/ml) in PBS for 10 minutes. Sections were washed twice with deionized water for 5 minutes each and then mounted in anti-fade solution, Citifluor (Citifluor, London, UK). Slides were covered and sealed with nail polish, and then allowed to air-dry for 10 minutes. Immunogold-labelling techniques at the electron microscopy level were modified based on indirect immunogold labelling of ultra-thin sections for TEM (Cole, 1996). Ultra-thin sections from LR gold blocks were placed on pioloform film coated nickel grids (100 hexagonal mesh) and were blocked in 30 μl/grid of blocking buffer containing phosphate buffered saline plus Tween 20 (PBST) (0.8 % NaCl, 0.02% KCl, 0.115% Na2HPO4, KH2PO4, 0.02% NaN3 and 0.5% Tween 20, pH6.9) and 1% BSA at room temperature for 1 hour. Then the sections were incubated in 20μl/grid of 1:100 primary antibodies for callose and callose synthase and 1:400 for β-1,3-glucanase in the blocking buffer at room temperature for 2 hours. Sections were washed with PBST 3 times for 5 minutes each, followed by incubation with secondary antibody (1:30 dilution with the blocking buffer) at room temperature for 1 hour. Sections were washed again 3 times with PBST and then with distilled water twice all for 5 minutes each, followed by background staining with saturated uranyl acetate (in 50% ethanol) and Reynold’s lead citrate for 10 minutes each. The sections were rinsed twice with distilled water for 1 minute after staining.
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I am posting the following for a colleague who has difficulty with his computer. Please reply to microscopy so that everyone can read it, including him.
We are using high-pressure freezing along with freeze substitution to try preserving the structure of Arabidopsis pollen. One of the challenges is preserving the pollen kitt (tryphine) on the surface of the pollen which mostly comprises of lipids and sterol-esters. We have attempted to use just paraformaldehyde (2%) and glutaraldehyde (0.2%) in the freeze substitution fixative in the hopes that the proteinaceous components of the pollen kitt would be sufficiently fixed to retain its structure after embedding but this has clearly shown to not be the case.
Our final option would be to try varying concentrations of osmium (which is known to fix lipid components) but we are very concerned about loss of immunogenicity (which we will require from the protocol).
Does anyone have any experience with the use of osmium in low temperature freeze substitution protocols where immunogenicity is adequately preserved? We would like some feedback from the community concerning the range of concentrations that we could try as well as the compatibility of osmium with other fixatives (paraformaldehyde, glutaraldehyde). The last point is expecially important because one of our samples is whole anther and the last thing we need is black osmium metal ppt in an enclosed space.
Thank you kindly for any suggestions,
==============================Original Headers============================== 46, 29 -- From RHBerg-at-danforthcenter.org Tue May 10 16:06:22 2011 46, 29 -- Received: from spm1.danforthcenter.org (spm1.danforthcenter.org [65.254.111.26]) 46, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4AL6Lkx024168 46, 29 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2011 16:06:22 -0500 46, 29 -- Received: from spm1.danforthcenter.org (127.0.0.1) by spm1.danforthcenter.org (MlfMTA v3.2r9) id hp6kcq0171sn for {microscopy-at-microscopy.com} ; Tue, 10 May 2011 16:06:21 -0500 (envelope-from {RHBerg-at-danforthcenter.org} ) 46, 29 -- Received: from spm1.danforthcenter.org ([10.101.0.44]) 46, 29 -- by spm1.danforthcenter.org (SonicWALL 7.2.4.3927) 46, 29 -- with ESMTP (AIO); Tue, 10 May 2011 16:06:21 -0500 46, 29 -- Received: from mail2.ddpsc.org ([::1]) by mail2.ddpsc.org ([::1]) with mapi; 46, 29 -- Tue, 10 May 2011 16:06:21 -0500 46, 29 -- From: "Berg, R. Howard" {RHBerg-at-danforthcenter.org} 46, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 46, 29 -- Date: Tue, 10 May 2011 16:06:20 -0500 46, 29 -- Subject: [Microscopy] Re: Osmium, freeze substitution, and immonogenicity 46, 29 -- Thread-Topic: [Microscopy] Re: Osmium, freeze substitution, and 46, 29 -- immonogenicity 46, 29 -- Thread-Index: AcwPVhxXaVcyo4VRSrCgIU5mHnMtAA== 46, 29 -- Message-ID: {32B90E3D-33AA-4BB0-9148-D2412A884943-at-danforthcenter.org} 46, 29 -- Accept-Language: en-US 46, 29 -- Content-Language: en-US 46, 29 -- X-MS-Has-Attach: 46, 29 -- X-MS-TNEF-Correlator: 46, 29 -- acceptlanguage: en-US 46, 29 -- Content-Type: text/plain; charset="utf-8" 46, 29 -- MIME-Version: 1.0 46, 29 -- X-Mlf-Version: 7.2.4.3927 46, 29 -- X-Mlf-UniqueId: o201105102106210027771 46, 29 -- Content-Transfer-Encoding: 8bit 46, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p4AL6Lkx024168 ==============================End of - Headers==============================
Registration is now open for the Advanced Digital Imaging Workshop scheduled for June 20-23, 2011. Space is limited and the workshop may fill prior to registration closing. This workshop is appropriate for graduate students, research staff, post-docs and interested senior researchers. The Microscopy Facility operated by the Neuroscience Research Institute (NRI) and the Department of Molecular, Cellular, and Developmental Biology at UC Santa Barbara, offers this workshop to provide intensive training with light microscopy. This workshop includes three and a half days of seminars and hands on instruction. Through the course, participants will gain theoretical and practical experience with light microscopy. Lectures will focus on microscopy principals and applications while laboratories will focus on collecting quality images and data for research. Fixed biological sample will be used in this course although many topics will be relevant for non-biological samples and live cell imaging. This seminar serves as an excellent foundation for our live cell imaging workshop.
For more information and to register see https://www.lifesci.ucsb.edu/mcdb/events/imaging_workshop/
-- Mary Raven Microscopy Facility Director Neuroscience Research Institute& Molecular, Cellular& Developmental Biology Biology 2 Building, room 5173 The University of California Santa Barbara, CA 93106-5060
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both edith.stabentheiner-at-uni-graz.at as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I wanted to test HMDS (hexamethyldisilazane) as an alternative to CPD for SEM of plant material and insects. 100 % HMDS was not available and we got only 98% (greater or equal). Nonetheless, I prepared a sample and everything seemed to be fine. Drying was ok and there was no problem with the vacuum. However, I observed a slight discolouration of the gold target during sputter coating - the non sputtered gold surface turned greyish. Do you have experienced a similar phenomen and can you explain it to me? Is it a consequence of bad drying and/or the wrong quality of HMDS? Thank you for your help!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mike.net.mo-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mike.net.mo-at-gmail.com Name: Xiaoming Fan
Organization: Forschungszentrum Jülich
Title-Subject: [Filtered] How to fix the fluoreschence microspheres to the coverslip
Message: We want to use the 200nm fluospheres microspheres to fix it on the surface of the coverslip as a reference for measurement, but we have a problem that micrspheres just flow in the solutions which is on the surface of the coverslip, we don't know whether there is a method to fix the microspheres and meanwhile not influence the fluorescence character of the microspheres, our microspheres are from Invitrogen, and the product No is: (F8811) Fluospheres carboxylate-modified microspheres, 200 nm, yellow/green fluorescent (505/515). Thanks very much!
We use 98% pure HMDS and have never noticed discolouration of our gold target.
I would like to open out the discussion. Should I look for greater than HMDS of greater than 98% purity for dehydration of cultured human cell lines?
Dave
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 11 May 2011 13:17 To: David Patton
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both edith.stabentheiner-at-uni-graz.at as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I wanted to test HMDS (hexamethyldisilazane) as an alternative to CPD for SEM of plant material and insects. 100 % HMDS was not available and we got only 98% (greater or equal). Nonetheless, I prepared a sample and everything seemed to be fine. Drying was ok and there was no problem with the vacuum. However, I observed a slight discolouration of the gold target during sputter coating - the non sputtered gold surface turned greyish. Do you have experienced a similar phenomen and can you explain it to me? Is it a consequence of bad drying and/or the wrong quality of HMDS? Thank you for your help!
We use 98-99% HMDS and have not noticed any discoloration of our sputter coater targets. It may be that you did not completely remove the ethanol, or did not completely dry the sample from HMDS. How did the pump-down go in the sputter coater? Did you have trouble getting to vacuum at first? Did you do a few purges with argon before coating in order to completely flush out all the air and any adsorbed water vapor, etc., from the chamber?
Phil
} Email: edith.stabentheiner-at-uni-graz.at Name: Edith Stabentheiner } } Organization: University of Graz } } Title-Subject: [Filtered] Quality of HMDS } } Message: Dear colleagues } } I wanted to test HMDS (hexamethyldisilazane) as an alternative to CPD } for SEM of plant material and insects. 100 % HMDS was not available and } we got only 98% (greater or equal). Nonetheless, I prepared a sample and } everything seemed to be fine. Drying was ok and there was no problem } with the vacuum. However, I observed a slight discolouration of the gold } target during sputter coating - the non sputtered gold surface turned } greyish. Do you have experienced a similar phenomen and can you explain } it to me? Is it a consequence of bad drying and/or the wrong quality of } HMDS? } Thank you for your help! } } Edith Stabentheiner } Graz, Austria
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 30 -- From oshel1pe-at-cmich.edu Wed May 11 09:26:51 2011 4, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4BEQpri012254 4, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2011 09:26:51 -0500 4, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p4BEQeOQ002293; 4, 30 -- Wed, 11 May 2011 10:26:49 -0400 4, 30 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 4, 30 -- Wed, 11 May 2011 10:26:42 -0400 4, 30 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Wed, 11 May 4, 30 -- 2011 10:26:42 -0400 4, 30 -- MIME-Version: 1.0 4, 30 -- Message-ID: {a06240802c9f04c6e0db0-at-[141.209.160.249]} 4, 30 -- In-Reply-To: {201105111216.p4BCGmg6004569-at-ns.microscopy.com} 4, 30 -- References: {201105111216.p4BCGmg6004569-at-ns.microscopy.com} 4, 30 -- Date: Wed, 11 May 2011 10:26:40 -0400 4, 30 -- To: {edith.stabentheiner-at-uni-graz.at} 4, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 30 -- Subject: Re: [Microscopy] viaWWW:Quality of HMDS 4, 30 -- CC: {Microscopy-at-microscopy.com} 4, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 30 -- X-Originating-IP: [141.209.160.249] 4, 30 -- X-OriginalArrivalTime: 11 May 2011 14:26:42.0873 (UTC) FILETIME=[7308F290:01CC0FE7] 4, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 30 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 4, 30 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 30 -- X-CanItPRO-Stream: default 4, 30 -- X-Canit-Stats-ID: 02EGeqNKP - 4bb58d4b4c0c - 20110511 4, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I believe the discoloration was the result of incomplete drying of your sample, but could also be due to sample outgassing. You may need to extend your dehydration steps. What I recommend, also, is to place samples in a 45 degree C vacuum oven after HMDS and pull a vacuum on the sample overnight to insure the sample is completely dry if you are working with a thick or difficult sample. A Graduate Student in my lab is working with shark skin, which contains a lot of connective tissue. She was unable to coat 6 samples at a time in my sputter coater. There was too much sample outgassing with her samples even with the overnight baking in the oven. With the 6 samples in the chamber, our gold-palladium target discolored, also, and her samples never coated properly, although a plasma formed in the chamber. She asked me to try another run with the coater, and I had the same results as she did with another set of samples. I noticed that I needed almost no argon gas to produce a plasma. At first, I thought I was having a problem with my gas feed and disassembled my needle valve and cleaned it, but that was not the case. The same phenomenon occurred with the next set of samples. These were samples that had been processed, oven dried and been in a bell jar over dessicant for weeks. This led me to conclude that the samples were outgassing. Extended baking at 45 degrees did not cure the problem. When we reduced the sample number to 3 in the chamber, all three samples coat fine. Try placing fewer samples in the chamber with each coating run as well.
Ed Haller
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, May 11, 2011 8:22 AM To: Haller, Edward
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both edith.stabentheiner-at-uni-graz.at as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I wanted to test HMDS (hexamethyldisilazane) as an alternative to CPD for SEM of plant material and insects. 100 % HMDS was not available and we got only 98% (greater or equal). Nonetheless, I prepared a sample and everything seemed to be fine. Drying was ok and there was no problem with the vacuum. However, I observed a slight discolouration of the gold target during sputter coating - the non sputtered gold surface turned greyish. Do you have experienced a similar phenomen and can you explain it to me? Is it a consequence of bad drying and/or the wrong quality of HMDS? Thank you for your help!
---------------------------------------------------------------------------- --- Paid PhD position in microstructural characterization by TEM ---------------------------------------------------------------------------- ---
Location: Aristotle University of Thessaloniki, Thessaloniki, Greece
Duration: fixed appointment of 36 months
Framework: European Marie Curie ITN project NetFISiC
Job Description: Work on the characterization by Transmission Electron Microscopy (TEM) of the samples provided by the network institutions. Participation in project meeting. Numerous training opportunities (schools, conferences, secondments, etc). Previous experience on TEM characterization will be considered a great asset, but is not strictly required.
Salary: Generous package under European Marie Curie regulations (salary + rent + travel + conferences and meetings + career exploratory allowance)
Full details can be found at: http://ec.europa.eu/euraxess/index.cfm/jobs/jobDetails/33689063
Please forward this to anyone you think might be interested.
Prof. E.K. Polychroniadis Department of Physics Aristotle University of Thessaloniki Thessaloniki 54124, Greece Tel.: +30.2310.998163 Fax: +30.2310.998241 e-mail: polychr-at-auth.gr
==============================Original Headers============================== 13, 21 -- From polychr-at-auth.gr Wed May 11 10:16:02 2011 13, 21 -- Received: from hermes3.ccf.auth.gr (hermes3.ccf.auth.gr [155.207.1.69]) 13, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4BFG1Ik012600 13, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2011 10:16:01 -0500 13, 21 -- Received: from Kerasia (adsl-251-077.diodos.auth.gr [155.207.251.77]) 13, 21 -- by hermes3.ccf.auth.gr (8.14.4/8.14.4) with ESMTP id p4BFFx3K004117 13, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2011 18:15:59 +0300 13, 21 -- From: "Polychroniadis Stathis" {polychr-at-auth.gr} 13, 21 -- To: {Microscopy-at-microscopy.com} 13, 21 -- Subject: Position available: Paid PhD in microstructural characterization by TEM 13, 21 -- Date: Wed, 11 May 2011 18:15:56 +0300 13, 21 -- Message-ID: {00a401cc0fee$543ed9e0$fcbc8da0$-at-gr} 13, 21 -- MIME-Version: 1.0 13, 21 -- Content-Type: text/plain; 13, 21 -- charset="us-ascii" 13, 21 -- Content-Transfer-Encoding: 7bit 13, 21 -- X-Mailer: Microsoft Office Outlook 12.0 13, 21 -- Thread-Index: AcwP7lO0wTB0Hk9oRN6apvQ6rlryxA== 13, 21 -- Content-Language: el 13, 21 -- X-Virus-Scanned: clamav-milter 0.97 at hermes1 13, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
We are working with "Quantitative Analysis Software" (QAS) for an EDS detector. The software is used both for auto identification of peaks and for quantitation. The software was apparently developed for use with an X-ray excitation source but may be usable also for electron beam excitation. In our case the electron beam is a cold cathode source with a nominal beam energy of 20 kV. Because of the nature of the cold cathode electron beam source, there is an appreciable energy spread in the exciting beam (as contrasted with more elaborate sources on SEMs, etc.)
The samples for the most part are geological (rock thin sections and thin slabs).
If anyone has any experience or references to work in this area, we would be pleased to hear about them.
Thank you, Travis Johnson
-- Travis Johnson Bedford MA
==============================Original Headers============================== 7, 30 -- From travisj25-at-gmail.com Wed May 11 14:26:27 2011 7, 30 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4BJQQCd032536 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 11 May 2011 14:26:27 -0500 7, 30 -- Received: by iwg8 with SMTP id 8so1108717iwg.0 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 11 May 2011 12:26:26 -0700 (PDT) 7, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 30 -- d=gmail.com; s=gamma; 7, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 7, 30 -- :content-type; 7, 30 -- bh=JqancoZtcgX5mSAQHuzgNhCy3Sa8vUc9zk97az795Mo=; 7, 30 -- b=esm2aAX/dp1gstybkrsOgtXPS77RZim3hQdGdxXSQDUSOcoHg4Ccm1Q4yNcrUd4C2Q 7, 30 -- 5k87Pt++TJ4OJRkK5LGAYfhYSM/u0h35Q9NIpGWdifThjcpWKPoJQ91QFP3TDfgCme3j 7, 30 -- WIHgq/hgJlBkXgiCRPv83amkecKDW+DN5oYZo= 7, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 7, 30 -- d=gmail.com; s=gamma; 7, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 7, 30 -- b=V67g9h42uAZiuD89jNQGsZAkU0HUnmUJjEiHDrfZ3YyoTTtZXOloppycOznvhfasQr 7, 30 -- +xAMvf5jbsphD3cPtNFhKAqq/qmkOHc5jphFizQQkvH8m1vEMhoTgR9ifiW66QsMIZI1 7, 30 -- usbiI2G2LBrSj6M8a6xNjP37jIK6S9L6nmoT0= 7, 30 -- MIME-Version: 1.0 7, 30 -- Received: by 10.42.131.194 with SMTP id a2mr47503ict.229.1305141982764; Wed, 7, 30 -- 11 May 2011 12:26:22 -0700 (PDT) 7, 30 -- Received: by 10.231.145.147 with HTTP; Wed, 11 May 2011 12:26:22 -0700 (PDT) 7, 30 -- Date: Wed, 11 May 2011 15:26:22 -0400 7, 30 -- Message-ID: {BANLkTimB-5_goc6Qp-F3T-K_EJkkOs+CFA-at-mail.gmail.com} 7, 30 -- Subject: EDS - Electron Beam Excitation 7, 30 -- From: Travis Johnson {travisj25-at-gmail.com} 7, 30 -- To: microscopy-at-microscopy.com 7, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Does anyone in the EM community know of groups working on biological aspects of silica?
I have a PhD student who's been working on general eukaryotic mechanisms of silica transport, starting to look around for potential collaborations for postdocs. My background is taxonomy and systematics, so I don't really know the cell biological or materials science communities. Any organisms (animals, choanoflagellates, plants, big & small brown algae, radiolarians, euglyphids....), and any timescale (palaeontology as well as current molecular work as well as future biotechnology) would be fine.
thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
Hi, I have a 15 month old Haskris R100 Air Cooled Water Chiller, 208V available. My new lab has chilled water to cool a water cooled water chiller (sounds a bit redundant doesn't it?) Contact me directly if you are interested in a swap for some supplies. Shipping costs from San Francisco will be your responsibility.
Thanks, Larry
-- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes& Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S1355 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
larry.ackerman-at-ucsf.edu
415-999-4758
==============================Original Headers============================== 6, 34 -- From btv1==1133fc662cb==Larry.Ackerman-at-ucsf.edu Wed May 11 19:05:29 2011 6, 34 -- Received: from bcuda1.ucsf.edu (bcuda1.ucsf.edu [64.54.247.213]) 6, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4C05TYM004087 6, 34 -- for {microscopy-at-microscopy.com} ; Wed, 11 May 2011 19:05:29 -0500 6, 34 -- X-ASG-Debug-ID: 1305158728-02475d2df9344110001-4CH8be 6, 34 -- Received: from exht04.net.ucsf.edu (mx.ucsf.edu [64.54.247.193]) by bcuda1.ucsf.edu with ESMTP id gUMJ3AvN49HMqPkf (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 11 May 2011 17:05:28 -0700 (PDT) 6, 34 -- X-Barracuda-Envelope-From: Larry.Ackerman-at-ucsf.edu 6, 34 -- X-Barracuda-Apparent-Source-IP: 64.54.247.193 6, 34 -- Received: from [192.168.1.119] (128.218.108.218) by exht04.net.ucsf.edu 6, 34 -- (64.54.247.221) with Microsoft SMTP Server id 14.1.289.1; Wed, 11 May 2011 6, 34 -- 17:05:27 -0700 6, 34 -- Message-ID: {4DCB2447.3030508-at-ucsf.edu} 6, 34 -- Date: Wed, 11 May 2011 17:05:27 -0700 6, 34 -- From: Larry Ackerman {larry.ackerman-at-ucsf.edu} 6, 34 -- Reply-To: {larry.ackerman-at-ucsf.edu} 6, 34 -- Organization: Microscopy Core, UCSF 6, 34 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 6, 34 -- MIME-Version: 1.0 6, 34 -- To: MSA Listserver {microscopy-at-microscopy.com} 6, 34 -- Subject: Air Cooled Water Chiller 6, 34 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 6, 34 -- X-ASG-Orig-Subj: Air Cooled Water Chiller 6, 34 -- Content-Transfer-Encoding: 7bit 6, 34 -- X-Barracuda-Connect: mx.ucsf.edu[64.54.247.193] 6, 34 -- X-Barracuda-Start-Time: 1305158728 6, 34 -- X-Barracuda-Encrypted: AES128-SHA 6, 34 -- X-Barracuda-URL: http://bcuda1.ucsf.edu:80/cgi-mod/mark.cgi 6, 34 -- X-Virus-Scanned: by bsmtpd at ucsf.edu 6, 34 -- X-Barracuda-Spam-Score: 0.00 6, 34 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=9.0 KILL_LEVEL=5.0 tests= 6, 34 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.63468 6, 34 -- Rule breakdown below 6, 34 -- pts rule name description 6, 34 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
Can anyone suggest solutions (pun probably intended) to the problem of mystery precipitate on cells prepared for TEM? This is 5nm- 15 nm diameter black dots that appear on cells, but not on resin sections or on support film. The dots localise strongly to chloroplasts and myelin when osmium is involved, but not otherwise. In glutaraldehyde-only fixes the dots stay on the outside of the cells or in vacuoles.
Many people have posted before about precipitates; on the basis of having gone through the Listserver archives we decided to try a series of experiments to try and get rid of the mystery 5nm dots currently gracing all cells prepared for TEM in this lab. Our results are inconclusive, thus we're wondering if anyone else has tried anything and found a way of making the dots disappear.
I have tried many combinations and permutations of local/ bought water, 3 different purities of glutaraldehyde, paraformaldehyde with and without glut, new osmium, lots of different buffers, new glassware, plastic containers instead of glassware... the only thing i haven't tried yet is the ruthenium/ veronal acetate fixes...
Anyone with time to have a look can find details of some of the experiments in the pdf here, {http://dl.dropbox.com/u/10613310/PrecipLIST-12May2011.pdf} and these are representative of other results with different buffers. Obviously the cells aren't particularly nicely fixed - that's another set of experiments...
Any suggestions most gratefully received
thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
Dear All, someone has just asked me which companies provide add-on WDX systems for SEMs, they are looking to purchase a system. I can think of a few (e.g. Edax, Oxford Instruments, Thermo) , but I'm not confident that I know all of them - for example I would have thought Bruker make a system but there's nothing on their website. Any ideas? I would also be grateful for feedback on system performance, ease of use, quality of service response etc. from anyone who has one of these systems which might help make a decision on a provider.
Many thanks indeed
Richard Beanland
==============================Original Headers============================== 3, 27 -- From contact-at-integrityscientific.com Thu May 12 03:13:49 2011 3, 27 -- Received: from mo-p01-ob.rzone.de (mo-p01-ob.rzone.de [81.169.146.165]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4C8DnC2009633 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 03:13:49 -0500 3, 27 -- DKIM-Signature: v=1; a=rsa-sha1; c=relaxed/relaxed; t=1305188028; l=591; 3, 27 -- s=domk; d=integrityscientific.com; 3, 27 -- h=Content-Transfer-Encoding:Content-Type:Subject:To:MIME-Version:From: 3, 27 -- Date:X-RZG-CLASS-ID:X-RZG-AUTH; 3, 27 -- bh=vmag5tfyVUpxmiZC+3gNK2dc4F0=; 3, 27 -- b=wLUmzq8nulZCjq+SdYQfkFv6gLibHcPe67d7bEc7J1ziPzyO3m8m0ayirEe/9wxT/dP 3, 27 -- oITSixo7VLtcMJgWeiVZ2Oyv8W4KUt5RSaAqYT6jBMP35dlGp43XLzts8Aj8H0zCC3KDm 3, 27 -- iN2VUTTk3DIG6hiyUQUKGWJInKDPOr3faJc= 3, 27 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSd5rS69964oI29h2ZepZS93fbcBQpvfRt/n9g== 3, 27 -- X-RZG-CLASS-ID: mo01 3, 27 -- Received: from [127.0.0.1] ([137.205.62.100]) 3, 27 -- by post.strato.de (fruni mo24) (RZmta 25.18) 3, 27 -- with ESMTPA id v036b0n4C6Wjqc for {microscopy-at-microscopy.com} ; 3, 27 -- Thu, 12 May 2011 10:13:47 +0200 (MEST) 3, 27 -- Message-ID: {4DCB96BD.5050403-at-integrityscientific.com} 3, 27 -- Date: Thu, 12 May 2011 09:13:49 +0100 3, 27 -- From: Richard Beanland {contact-at-integrityscientific.com} 3, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-GB; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 3, 27 -- MIME-Version: 1.0 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: WDX providers 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I got similar precipitate when I didn't wash out the PBS enough before block-contrasting in Uranyl acetate. Also, I got this type of precipitate when I left my samples on Osmium too long (} 3h, and they were not the ones with the PBS not washed :) ) If you mix PBS (or any other phosphate) with Uranyl, you will get fine precipitate. I am not sure about the mechanism of precipitate forming after long(er) Osmium exposure.
I read one paper where the author identified Calcium (intracellular) as black precipitate, but concentrated in mitochondria and not diffused. But I don't remember the paper, nor the author, and I am not sure how accurate is that identification.
Maybe someone else has more and other ideas regarding the precipitate.
Hope it helps,
Josif
On Thu, 12 May 2011 00:41:58 -0500, giselle.walker-at-anatomy.otago.ac.nz wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver [1] } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } [2] } ---------------------------------------------------------------------------- } } Can anyone suggest solutions (pun probably intended) to the problem } of } mystery precipitate on cells prepared for TEM? This is 5nm- 15 nm } diameter black dots that appear on cells, but not on resin sections } or on } support film. The dots localise strongly to chloroplasts and myelin } when } osmium is involved, but not otherwise. In glutaraldehyde-only fixes } the } dots stay on the outside of the cells or in vacuoles. } } Many people have posted before about precipitates; on the basis of } having } gone through the Listserver archives we decided to try a series of } experiments to try and get rid of the mystery 5nm dots currently } gracing } all cells prepared for TEM in this lab. Our results are inconclusive, } thus we're wondering if anyone else has tried anything and found a } way of } making the dots disappear. } } I have tried many combinations and permutations of local/ bought } water, 3 } different purities of glutaraldehyde, paraformaldehyde with and } without } glut, new osmium, lots of different buffers, new glassware, plastic } containers instead of glassware... the only thing i haven't tried yet } is } the ruthenium/ veronal acetate fixes... } } Anyone with time to have a look can find details of some of the } experiments in the pdf here, } } and these are representative of other results with different buffers. } Obviously the cells aren't particularly nicely fixed - that's another } set } of experiments... } } Any suggestions most gratefully received } } thanks } } Giselle } } -- } Dr Giselle Walker } Microscopy Otago - Electron Microscopy } Department of Anatomy & Structural Biology } University of Otago } P.O. Box 913, Dunedin 9054 } New Zealand } } Phone: +64 (0)3 479 7301, +64 (0)210 403 669 } Email: giselle.walker-at-anatomy.otago.ac.nz [4]
The dreaded black dot plague! I've struggled with this precipitate here in Tampa for years. It seems that our groundwater in Florida is rich in phosphates, and that the Millipore and similar filtration units don't always take all of the phosphates out of the water when the systems filter the water. This results in phosphate microcrystal precipitates in tissue processed for TEM if I use filtered water. When I switched to true distilled water, this issue resolved itself. I now use true distilled water to make my reagents in, and for my rinses. I work with phosphate buffered fixatives, which preserve the cytosol of mammalian tissue better. After glutaraldehyde fixation, I do a half hour buffer rinse, 3x 10 minutes each, minimum, before osmication, and even longer if I am processing tissue such as myelinated nerve, in which case I rinse overnight in buffer. After osmication, I rinse in distilled water, again 3x 10 minutes each, minimum, and longer if the tissue is thick or dense, to remove both the osmium and the phosphate salts. This has cured the precipitate problems for me. Some advice I gave another E.M. technician was to rinse in Tris buffer after glutaraldehyde and osmicate in Tris. The two buffer systems are compatible. You could consider this, also. Let me know what you find out. If you switch to cacodylate buffer, you should not get this precipitate, if phosphate salts are the culprit. I don't know if this is a possibility for you. I don't like the look of my tissue when working with cacodylate buffer, and the arsenic in the buffer is something I try to avoid.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: giselle.walker-at-anatomy.otago.ac.nz [giselle.walker-at-anatomy.otago.ac.nz] Sent: Thursday, May 12, 2011 1:40 AM To: Haller, Edward
Can anyone suggest solutions (pun probably intended) to the problem of mystery precipitate on cells prepared for TEM? This is 5nm- 15 nm diameter black dots that appear on cells, but not on resin sections or on support film. The dots localise strongly to chloroplasts and myelin when osmium is involved, but not otherwise. In glutaraldehyde-only fixes the dots stay on the outside of the cells or in vacuoles.
Many people have posted before about precipitates; on the basis of having gone through the Listserver archives we decided to try a series of experiments to try and get rid of the mystery 5nm dots currently gracing all cells prepared for TEM in this lab. Our results are inconclusive, thus we're wondering if anyone else has tried anything and found a way of making the dots disappear.
I have tried many combinations and permutations of local/ bought water, 3 different purities of glutaraldehyde, paraformaldehyde with and without glut, new osmium, lots of different buffers, new glassware, plastic containers instead of glassware... the only thing i haven't tried yet is the ruthenium/ veronal acetate fixes...
Anyone with time to have a look can find details of some of the experiments in the pdf here, {http://dl.dropbox.com/u/10613310/PrecipLIST-12May2011.pdf} and these are representative of other results with different buffers. Obviously the cells aren't particularly nicely fixed - that's another set of experiments...
Any suggestions most gratefully received
thanks
Giselle
-- Dr Giselle Walker Microscopy Otago - Electron Microscopy Department of Anatomy & Structural Biology University of Otago P.O. Box 913, Dunedin 9054 New Zealand
Back in the dark ages, in the 1980s, we had a Microspec WDX-2A on our SEM. We had originally bought it for light element analyses because our EDS could not get below sodium. Since we got a light element EDS detectors we found little need for it. There was one occasion we used it to confirm the EDS-measured ratio of Pb and Bi in a sample. Basically it fell into disuse.
Granted, that system was quite limited and a bit cumbersome not having the computerization that is available today. That could have made a difference if we could have easily incorporated WDS measurements with EDS measurements.
We also had to deal with different requirements for WDS and EDS. We had a Si(Li) detector that was hitting 30% deadtime at 1500 cps. The WDS was barely registering counts at those conditions. If we cranked up the beam to get good WDS counts, the EDS detector had to be retracted and/or used at short processing times to keep the deadtime manageable. I know that issue is less now with the new SDD systems.
I wonder what your colleague has in mind for WDX and if it is worth the trouble and expense to pursue the matter. I am spending half of my time on a microprobe now, so I know there is definitely a place for WDS. I'm sure the vendors could cite some examples of where the combined system is useful. I just hope your colleague has thought it well through. I spent nearly 30 years working with EDS alone and know it can be quite useful. With new developments it is getting all the more useful.
Warren Straszheim
-----Original Message----- X-from: contact-at-integrityscientific.com [mailto:contact-at-integrityscientific.com] Sent: Thursday, May 12, 2011 3:15 AM To: wesaia-at-iastate.edu
Does anyone have experience with the microcalorimetry EDS detector (MICA1600) from STAR Cryoelectronics? I know it is a high cost addition but it seems to improve the energy resolution of EDS making it competitive with WDS.
John A. Robson Research Scientist Boehringer Ingelheim Pharmaceuticals, Inc. (203)798-5640
-----Original Message----- X-from: contact-at-integrityscientific.com [mailto:contact-at-integrityscientific.com] Sent: Thursday, May 12, 2011 4:22 AM To: Robson,John (AN) BIP-US-R
Dear All, someone has just asked me which companies provide add-on WDX systems for SEMs, they are looking to purchase a system. I can think of a few (e.g. Edax, Oxford Instruments, Thermo) , but I'm not confident that I know all of them - for example I would have thought Bruker make a system but there's nothing on their website. Any ideas? I would also be grateful for feedback on system performance, ease of use, quality of service response etc. from anyone who has one of these systems which might help make a decision on a provider.
Many thanks indeed
Richard Beanland
==============================Original Headers============================== 3, 27 -- From contact-at-integrityscientific.com Thu May 12 03:13:49 2011 3, 27 -- Received: from mo-p01-ob.rzone.de (mo-p01-ob.rzone.de [81.169.146.165]) 3, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4C8DnC2009633 3, 27 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 03:13:49 -0500 3, 27 -- DKIM-Signature: v=1; a=rsa-sha1; c=relaxed/relaxed; t=1305188028; l=591; 3, 27 -- s=domk; d=integrityscientific.com; 3, 27 -- h=Content-Transfer-Encoding:Content-Type:Subject:To:MIME-Version:From: 3, 27 -- Date:X-RZG-CLASS-ID:X-RZG-AUTH; 3, 27 -- bh=vmag5tfyVUpxmiZC+3gNK2dc4F0=; 3, 27 -- b=wLUmzq8nulZCjq+SdYQfkFv6gLibHcPe67d7bEc7J1ziPzyO3m8m0ayirEe/9wxT/dP 3, 27 -- oITSixo7VLtcMJgWeiVZ2Oyv8W4KUt5RSaAqYT6jBMP35dlGp43XLzts8Aj8H0zCC3KDm 3, 27 -- iN2VUTTk3DIG6hiyUQUKGWJInKDPOr3faJc= 3, 27 -- X-RZG-AUTH: :L2MKYUGrb9+u5owo+jDbZQFQdONAbZjDx+2vFy606k0cBSd5rS69964oI29h2ZepZS93fbcBQpvf Rt/n9g== 3, 27 -- X-RZG-CLASS-ID: mo01 3, 27 -- Received: from [127.0.0.1] ([137.205.62.100]) 3, 27 -- by post.strato.de (fruni mo24) (RZmta 25.18) 3, 27 -- with ESMTPA id v036b0n4C6Wjqc for {microscopy-at-microscopy.com} ; 3, 27 -- Thu, 12 May 2011 10:13:47 +0200 (MEST) 3, 27 -- Message-ID: {4DCB96BD.5050403-at-integrityscientific.com} 3, 27 -- Date: Thu, 12 May 2011 09:13:49 +0100 3, 27 -- From: Richard Beanland {contact-at-integrityscientific.com} 3, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-GB; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 3, 27 -- MIME-Version: 1.0 3, 27 -- To: microscopy-at-microscopy.com 3, 27 -- Subject: WDX providers 3, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 3, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 12, 28 -- From prvs=106a1f5e5=john.robson-at-boehringer-ingelheim.com Thu May 12 09:56:44 2011 12, 28 -- Received: from mail1.boehringer-ingelheim.com (mail1.boehringer-ingelheim.com [148.188.9.170]) 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4CEuhu0015803 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:56:43 -0500 12, 28 -- Received: from nahexb01.am.boehringer.com ([10.128.98.121]) by RDGEXH01.boehringer.com with Microsoft SMTPSVC(6.0.3790.4675); 12, 28 -- Thu, 12 May 2011 10:56:18 -0400 12, 28 -- Received: from nahexm105.am.boehringer.com ([10.128.98.84]) by nahexb01.am.boehringer.com with Microsoft SMTPSVC(6.0.3790.4675); 12, 28 -- Thu, 12 May 2011 10:56:16 -0400 12, 28 -- x-mimeole: Produced By Microsoft Exchange V6.5 12, 28 -- Content-class: urn:content-classes:message 12, 28 -- MIME-Version: 1.0 12, 28 -- Content-Type: text/plain; 12, 28 -- charset="us-ascii" 12, 28 -- Subject: RE: [Microscopy] WDX providers 12, 28 -- Date: Thu, 12 May 2011 10:55:41 -0400 12, 28 -- Message-ID: {18F7E98C5EF4664BBBD1144AFE1585125C0DED-at-nahexm105.am.boehringer.com} 12, 28 -- In-Reply-To: {201105120821.p4C8LlUG023780-at-ns.microscopy.com} 12, 28 -- X-MS-Has-Attach: 12, 28 -- X-MS-TNEF-Correlator: 12, 28 -- Thread-Topic: [Microscopy] WDX providers 12, 28 -- Thread-Index: AcwQfaN5demo55USSMG6kY7Ou3BerQANffHQ 12, 28 -- References: {201105120821.p4C8LlUG023780-at-ns.microscopy.com} 12, 28 -- From: {john.robson-at-boehringer-ingelheim.com} 12, 28 -- To: {contact-at-integrityscientific.com} 12, 28 -- Cc: {microscopy-at-microscopy.com} 12, 28 -- X-OriginalArrivalTime: 12 May 2011 14:56:16.0728 (UTC) FILETIME=[BEBF7D80:01CC10B4] 12, 28 -- Content-Transfer-Encoding: 8bit 12, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CEuhu0015803 ==============================End of - Headers==============================
ELECTRON MICROSCOPY AND ELECTRON PROBE ANALYSIS OF MITOCHONDRIAL CATION ACCUMULATION IN SMOOTH MUSCLE A . P . SOMLYO, A . V . SOMLYO, C . E . DEVINE, P . D . PETERS, and T . A . HALL
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: jmircheski-at-us.es [mailto:jmircheski-at-us.es] Sent: Thursday, May 12, 2011 6:12 AM To: Monson, Frederick
Hi Giselle,
I got similar precipitate when I didn't wash out the PBS enough before block-contrasting in Uranyl acetate. Also, I got this type of precipitate when I left my samples on Osmium too long (} 3h, and they were not the ones with the PBS not washed :) ) If you mix PBS (or any other phosphate) with Uranyl, you will get fine precipitate. I am not sure about the mechanism of precipitate forming after long(er) Osmium exposure.
I read one paper where the author identified Calcium (intracellular) as black precipitate, but concentrated in mitochondria and not diffused. But I don't remember the paper, nor the author, and I am not sure how accurate is that identification.
Maybe someone else has more and other ideas regarding the precipitate.
Hope it helps,
Josif
On Thu, 12 May 2011 00:41:58 -0500, giselle.walker-at-anatomy.otago.ac.nz wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver [1] } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } [2] } ---------------------------------------------------------------------------- } } Can anyone suggest solutions (pun probably intended) to the problem } of } mystery precipitate on cells prepared for TEM? This is 5nm- 15 nm } diameter black dots that appear on cells, but not on resin sections } or on } support film. The dots localise strongly to chloroplasts and myelin } when } osmium is involved, but not otherwise. In glutaraldehyde-only fixes } the } dots stay on the outside of the cells or in vacuoles. } } Many people have posted before about precipitates; on the basis of } having } gone through the Listserver archives we decided to try a series of } experiments to try and get rid of the mystery 5nm dots currently } gracing } all cells prepared for TEM in this lab. Our results are inconclusive, } thus we're wondering if anyone else has tried anything and found a } way of } making the dots disappear. } } I have tried many combinations and permutations of local/ bought } water, 3 } different purities of glutaraldehyde, paraformaldehyde with and } without } glut, new osmium, lots of different buffers, new glassware, plastic } containers instead of glassware... the only thing i haven't tried yet } is } the ruthenium/ veronal acetate fixes... } } Anyone with time to have a look can find details of some of the } experiments in the pdf here, } } and these are representative of other results with different buffers. } Obviously the cells aren't particularly nicely fixed - that's another } set } of experiments... } } Any suggestions most gratefully received } } thanks } } Giselle } } -- } Dr Giselle Walker } Microscopy Otago - Electron Microscopy } Department of Anatomy & Structural Biology } University of Otago } P.O. Box 913, Dunedin 9054 } New Zealand } } Phone: +64 (0)3 479 7301, +64 (0)210 403 669 } Email: giselle.walker-at-anatomy.otago.ac.nz [4]
We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training.
As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments.
Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic.
We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts.
This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not?
Hoping for a miracle,
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 13, 34 -- May 2011 09:47:24 -0700 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 34 -- Content-Type: text/plain; charset=us-ascii 13, 34 -- Subject: Microscope service strategies 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 13, 34 -- X-Mailer: Apple Mail (2.1084) 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 ==============================End of - Headers==============================
One of our colleagues recently sent us some STEM-EDS maps of a thin film sample and we're trying to find a viewer for the files. The data was recorded on a JEOL 2010F with a Thermo Noran Vantage EDX system and sent to us in a ".si" format. Are there any free viewers or utilities that we can use to look at the spectra and maps? Ideally we'd like to be able to export graphs and images, or at least raw data, for publication.
Any help would be greatly appreciated. Thanks!
-- Steven Spurgeon Dynamic Characterization Group Department of Materials Science & Engineering Drexel University
the service contract for a new microscope is worth trying to include in the price paid for at least about 3 years. During this time keeping a record of each and every little thing that goes wrong will be useful to whoever eventually takes over the maintenance and repair duties. A service contract costs on average about 10% of the purchase price, so a three year contract is a substantial saving that will please the finance people.
The service you get is very dependent on the people who work for the service departments of the individual companies; often cutting edge instruments are unknown quantities to the field service engineers. You don't want to waste money while the instrument is down for the engineer to be educated on-site; so try to get the first few years with a service contract (beyond the warranty period). Don't sign-off the instrument until you are happy that everything is working, the installation needs to settle the instrument, so try everything yourself before being satisfied.
Eventually though there is a time when you are going to be better off without paying the huge bills to the service manager. It has to happen and will depend on you and your staff, to take the brunt of the work, using instructions of friendly service engineers. I don't believe the stories about low priority, in fact I'd argue they want your money; and so long as you have the quotation matched to the purchase order, you are on the schedule, same as everyone else.
If you have a lot of instruments from one company and it makes financial sense to hire an ex-service engineer (it's down to respect they feel from you) it's a good route, if you get the right one. Parts are often sold from a separate office to the service manager, so again if you have the numbers and they have the stock you are going to be fine. For instance we wanted some resistors and didn't want to wait three months for delivery, so alternatives were found that we believe are as good.
Good luck
Rob Keyse
On 5/12/2011 12:54 PM, jkrupp-at-deltacollege.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 25 -- From rok210-at-lehigh.edu Thu May 12 12:38:25 2011 8, 25 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4CHcPYn017744 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 12:38:25 -0500 8, 25 -- Received: from [127.0.0.1] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 8, 25 -- (authenticated bits=0) 8, 25 -- by rain.cc.lehigh.edu (8.14.4/8.14.4) with ESMTP id p4CHcMne021735 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 8, 25 -- Thu, 12 May 2011 13:38:23 -0400 8, 25 -- Message-ID: {4DCC1B0E.2030901-at-lehigh.edu} 8, 25 -- Date: Thu, 12 May 2011 13:38:22 -0400 8, 25 -- From: Rob Keyse {rok210-at-lehigh.edu} 8, 25 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 8, 25 -- MIME-Version: 1.0 8, 25 -- To: jkrupp-at-deltacollege.edu 8, 25 -- CC: microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] Microscope service strategies 8, 25 -- References: {201105121654.p4CGsrj7032024-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201105121654.p4CGsrj7032024-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 7bit 8, 25 -- X-Antivirus: avast! (VPS 110512-0, 05/12/2011), Outbound message 8, 25 -- X-Antivirus-Status: Clean 8, 25 -- X-Virus-Scanned: clamav-milter 0.97 at rain.cc.lehigh.edu 8, 25 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Ironically we just last week were informed of an exploratory committee being set up to address the rather substantial service contract costs the Institution pays annually with tax dollars drying up-and Jonathan- you were tops of my list to call on. One of the measures under exploration is the hiring of individuals to provide service onsite. I have not heard of a budget academic type EM facility that has made this model cost effective but would be interested in discussing this with anyone out there who has.
Just examining EM's, my Lab has 4 basic units from 3 different manufacturers all under continuous contract and if we include the rest of the machines in very close proximity we have 7 basic from 4. And the whole place 10 from 5. I would love to hear from anyone who is making onsite service in the absence of a contract work; in an academic type setting; with instruments from multiple manufacturers driven by relatively inexperienced users.
Thanks for any input,
Scott Whittaker Chief NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
The SEM Lab move is complete and is now located in room EG13B. East Wing Ground floor.
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Thursday, May 12, 2011 12:49 PM To: Whittaker, Scott
Friends:
We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training.
As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments.
Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic.
We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts.
This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not?
Hoping for a miracle,
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 13, 34 -- May 2011 09:47:24 -0700 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 34 -- Content-Type: text/plain; charset=us-ascii 13, 34 -- Subject: Microscope service strategies 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 13, 34 -- X-Mailer: Apple Mail (2.1084) 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 29 -- From WHITTAKS-at-si.edu Thu May 12 13:24:53 2011 27, 29 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4CIOrt3001983 27, 29 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 13:24:53 -0500 27, 29 -- Received: from SI-MSEHUB-N01.US.SINET.SI.EDU (si-msehubi-01.us.sinet.si.edu [160.111.49.142]) 27, 29 -- by si-mailout04.si.edu (Postfix) with ESMTP id 2F7BC5D70; 27, 29 -- Thu, 12 May 2011 14:24:53 -0400 (EDT) 27, 29 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::58ac:1cff:44b0:524e]) by 27, 29 -- SI-MSEHUB-N01.US.SINET.SI.EDU ([fe80::34ec:9394:533a:63ad%11]) with mapi; 27, 29 -- Thu, 12 May 2011 14:24:52 -0400 27, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 27, 29 -- To: "'jkrupp-at-deltacollege.edu'" {jkrupp-at-deltacollege.edu} 27, 29 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 27, 29 -- Date: Thu, 12 May 2011 14:24:51 -0400 27, 29 -- Subject: RE: [Microscopy] Microscope service strategies 27, 29 -- Thread-Topic: [Microscopy] Microscope service strategies 27, 29 -- Thread-Index: AcwQxHPf8MiJ1t2HT9yLD8R7S5j7OwABiSOQ 27, 29 -- Message-ID: {038C04650A93A14381E944A473695F619399F10ACC-at-SI-MSEV03.US.SINET.SI.EDU} 27, 29 -- References: {201105121648.p4CGmfGc019233-at-ns.microscopy.com} 27, 29 -- In-Reply-To: {201105121648.p4CGmfGc019233-at-ns.microscopy.com} 27, 29 -- Accept-Language: en-US 27, 29 -- Content-Language: en-US 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- acceptlanguage: en-US 27, 29 -- Content-Type: text/plain; charset="iso-8859-1" 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CIOrt3001983 ==============================End of - Headers==============================
Service from the scope companies is always the best bet until the money runs out.
You need contracts of some type for if a scope runs into a big problem just one off service contract call can cost nearly as much as a year's contract. Perhaps if you can list the service calls that you've had over the last year and price the hourly rate for non-contract calls you can convince those who are questioning the cost that it is a good deal. Do not forget to add in transportation etc. for the clock starts running from the time the engineer leaves the office I believe, not when he arrives at your lab. Ask your friendly scope companies and they will give you the prices.
In my previous employment I had used third party services from 4 different companies after our scopes were old. We had a TEM and a SEM under one PI and his grant. I did not hear about money coming from other sources.
The best was a service tech who retired and started up his own business. This was great. He knew my TEM, the cost was less than the scope company and our old scope was no longer covered for parts anyway. Oh the days of dumpster diving when we heard of a scope being discarded! He had a major stroke from which he did not recover and that terminated our contract.
The service that followed him had some drawbacks in that the man had never serviced our particular TEM before and at times I was telling him how to fix it. It was written into our contract that someone (me) would assist as needed during all service calls. He died and I kept the scope going for another year before I could not fix the problems any more. The scope was just under 40 years old. I turned to borrowing time on a TEM in another department until my PI retired shortly there after.
Our older SEM did not pose a problem for the companies that serviced it. They came in a timely manner and were very good at keeping it in proper condition.
Maybe you can talk a company who has employed some of the Delta EM Graduates to sponsor a service contract for you as a tax deduction. It might work!
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
I always advise institutional users to stay with OEM service contract if they can, or to purchase a service contract from the reputable third-party service provider as a second choice.
In the beginning of this year I've heard from two industrial users of FIB equipment that they are not allowed to have a service contract with anybody and forced into "pay as you go" mode. Well, guess what - one of them already has her FIB "down" for over a month, and there is nothing I or anyone else can do about it until the institution shells over US$20K in parts and commits to pay for labor of changing them.
Think twice.
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/12/2011 12:48 PM, jkrupp-at-deltacollege.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 40 -- From vray-at-partbeamsystech.com Thu May 12 18:15:46 2011 6, 40 -- Received: from nm1.bullet.mail.sp2.yahoo.com (nm1.bullet.mail.sp2.yahoo.com [98.139.91.71]) 6, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CNFkFh005888 6, 40 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 18:15:46 -0500 6, 40 -- Received: from [98.139.91.67] by nm1.bullet.mail.sp2.yahoo.com with NNFMP; 12 May 2011 23:15:45 -0000 6, 40 -- Received: from [98.139.91.52] by tm7.bullet.mail.sp2.yahoo.com with NNFMP; 12 May 2011 23:15:45 -0000 6, 40 -- Received: from [127.0.0.1] by omp1052.mail.sp2.yahoo.com with NNFMP; 12 May 2011 23:15:45 -0000 6, 40 -- X-Yahoo-Newman-Id: 946558.98228.bm-at-omp1052.mail.sp2.yahoo.com 6, 40 -- Received: (qmail 8526 invoked from network); 12 May 2011 23:15:45 -0000 6, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305242145; bh=lceTGS4bC/t1pbs3ZYFzWwlaUci+KCLg0XgQc2dR0Ks=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=6ZVvKFzm1Mkww1RkdZJO8ssShMhAH2ZePqWHCgBpZqb/Ngkch29A8M0bIWyT2ec3Z1/7DT5dLZWOt9IfAcrprgZDN698+dP4mM9KLrUgf4MhJrsOgj0Rw+S7adSs8GLLo8qjTBfPZHIymIDMXYmnNGwRfI10NnkCLOZ7+a+e0LI= 6, 40 -- Received: from [192.168.2.31] (vray-at-75.68.231.25 with plain) 6, 40 -- by smtp115.biz.mail.sp1.yahoo.com with SMTP; 12 May 2011 16:15:45 -0700 PDT 6, 40 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 40 -- X-YMail-OSG: rErEEb0VM1kdVQpW6ZCVCNW3PapKAmyGedkEtCQAJ1GhYxy 6, 40 -- vRTeiDMvoizgx0oCZbbePPLGSbodTI.ZMg7kJLHsw1zOJoX9OHKmBZriHxP_ 6, 40 -- jQF0uPGDb2yd1RBuxyuCZl003YcgPEiJ847muTaJ5kgnOY1_hFBJCJV04b18 6, 40 -- 0o.8o9Tp2yWC4Eb4TibXDBDow.G9fwKRF__dzmf62AkaS1lnW9IkyRm5FLRG 6, 40 -- BZ5zeoXfmWdMfuJGRie55ySV2jR.k_FWLi96kHasjyi9YPrnNBu0lgM7VVge 6, 40 -- O4CcTnsFdSVMwWNkPj7W1jlMHXvz3yD8vXCQx5EKvmKkhxZh4OmZUuiIu0eE 6, 40 -- MuVFiadi4ga6u1B.TVr.qAUpPhjhluyiMvIhT8sefBmkiIIu8_Ch9Ich.0NA 6, 40 -- FwKCT8OJ2xsak1Eg70F_NnPiJmkjwcd56sl4M2K1TDrc5kjlIEhUS6ld7hjR 6, 40 -- y_ePRQSkFbMnY0.4zzwaXZtIRaNWLZg8xQiFTgzTPjnj41.fWtoVLvbBVtbo 6, 40 -- AvHRcRN7jeMdLLBDOV7WnGrUa2fW_mTOjjlaUICKTi2y4cJad5sTS1ChLSgu 6, 40 -- lDpStc3kVC8t4mndVl2OM2cIJp5o8YSDGY8f0wRtmdPqX2c_gH1cO30aI4ac 6, 40 -- dXgo7wkPv2uZs22FjDCeoN1Dnmu2tMiT6PFl3JUmQGXhDXWmxMTZFg7SgrLR 6, 40 -- TGSHKTLc5S_cJq7saJj9bYYIBfymn8hiLChmcVJwHbCLj2hOdv0c- 6, 40 -- X-Yahoo-Newman-Property: ymail-3 6, 40 -- Message-ID: {4DCC6A21.7090500-at-partbeamsystech.com} 6, 40 -- Date: Thu, 12 May 2011 19:15:45 -0400 6, 40 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 6, 40 -- Reply-To: vray-at-partbeamsystech.com 6, 40 -- Organization: PBS&T 6, 40 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 6, 40 -- MIME-Version: 1.0 6, 40 -- To: jkrupp-at-deltacollege.edu, microscopy-at-microscopy.com 6, 40 -- Subject: Re: [Microscopy] Microscope service strategies 6, 40 -- References: {201105121648.p4CGmOKN018604-at-ns.microscopy.com} 6, 40 -- In-Reply-To: {201105121648.p4CGmOKN018604-at-ns.microscopy.com} 6, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was wondering if anyone has issues with complete infiltration of dextran when used as a filler for high pressure freezing? Lately, I've been using 20% Dextran (MW 70,000), but the embedded blocks (in Embed-812) are very difficult to section. Although the sample itself seems well infiltrated, the surrounding dextran is extremely hydrophillic and doesn't section smoothly. Rather, the sections tend to crinkle up at the knife edge and never fully flatten. This has happened for the past 2 high pressure freezing runs, and I'm tempted to try a different cryo-protectant. I was wondering if the issue lies in the concentration or MW of the dextran we are using.
Thanks, Shannon
==============================Original Headers============================== 3, 25 -- From modla-at-dbi.udel.edu Thu May 12 20:47:09 2011 3, 25 -- Received: from newmail.dbi.udel.edu (newmail.dbi.udel.edu [128.175.253.7]) 3, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D1l9bA023456 3, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 20:47:09 -0500 3, 25 -- Received: from localhost (localhost [127.0.0.1]) 3, 25 -- by newmail.dbi.udel.edu (Postfix) with ESMTP id 850CA1E6D885 3, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 21:47:09 -0400 (EDT) 3, 25 -- X-Virus-Scanned: amavisd-new at newmail.dbi.udel.edu 3, 25 -- Received: from newmail.dbi.udel.edu ([127.0.0.1]) 3, 25 -- by localhost (newmail.dbi.udel.edu [127.0.0.1]) (amavisd-new, port 10024) 3, 25 -- with ESMTP id dE0IjX6F2Q8V for {Microscopy-at-microscopy.com} ; 3, 25 -- Thu, 12 May 2011 21:47:09 -0400 (EDT) 3, 25 -- Received: from newmail.dbi.udel.edu (newmail.dbi.udel.edu [128.175.253.7]) 3, 25 -- by newmail.dbi.udel.edu (Postfix) with ESMTP id 3F9C51E6D884 3, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 21:47:09 -0400 (EDT) 3, 25 -- Date: Thu, 12 May 2011 21:47:09 -0400 (EDT) 3, 25 -- From: Shannon Modla {modla-at-dbi.udel.edu} 3, 25 -- To: Microscopy-at-microscopy.com 3, 25 -- Subject: Dextran for HPF 3, 25 -- Message-ID: {e3389b56-4b00-49e8-82fb-2d8acb4645fd-at-newmail} 3, 25 -- Content-Type: text/plain; charset=utf-8 3, 25 -- Content-Transfer-Encoding: 7bit 3, 25 -- MIME-Version: 1.0 3, 25 -- X-Originating-IP: [76.99.182.176] 3, 25 -- X-Mailer: Zimbra 7.0.1_GA_3105 (ZimbraWebClient - SAF3 (Win)/7.0.1_GA_3105) ==============================End of - Headers==============================
We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same.
Dave
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: 12 May 2011 17:50 To: David Patton
Friends:
We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training.
As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments.
Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic.
We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts.
This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not?
Hoping for a miracle,
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 13, 34 -- May 2011 09:47:24 -0700 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 34 -- Content-Type: text/plain; charset=us-ascii 13, 34 -- Subject: Microscope service strategies 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 13, 34 -- X-Mailer: Apple Mail (2.1084) 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 23, 46 -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id C44681BB808D; Fri, 13 May 23, 46 -- 2011 09:58:50 +0000 (UTC) 23, 46 -- X-SpamScore: -14 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) 23, 46 -- X-Spam-TCS-SCL: 1:0 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe.ac.uk;RD:none;EFVD:NLI 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by mail92-am1 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 23, 46 -- (UTC) 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown [10.3.201.242]) by 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 23, 46 -- 09:58:50 +0000 (UTC) 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 23, 46 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Fri, 13 May 2011 23, 46 -- 10:57:55 +0100 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} 23, 46 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 23, 46 -- Subject: RE: [spam]* [Microscopy] Microscope service strategies 23, 46 -- Thread-Topic: [spam]* [Microscopy] Microscope service strategies 23, 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads.uwe.ac.uk} 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} 23, 46 -- Accept-Language: en-US, en-GB 23, 46 -- Content-Language: en-US 23, 46 -- X-MS-Has-Attach: 23, 46 -- X-MS-TNEF-Correlator: 23, 46 -- acceptlanguage: en-US, en-GB 23, 46 -- Content-Type: text/plain; charset="us-ascii" 23, 46 -- MIME-Version: 1.0 23, 46 -- X-OriginatorOrg: uwe.ac.uk 23, 46 -- Content-Transfer-Encoding: 8bit 23, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 ==============================End of - Headers==============================
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 } 23, 46 -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe.ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 } 23, 46 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 46 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 } 23, 46 -- Subject: RE: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Topic: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads.uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB } 23, 46 -- Content-Language: en-US } 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB } 23, 46 -- Content-Type: text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit } 23, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO5XVijSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Valery, Very well stated. The only thing I might add is that the customer with a service contract is guaranteed certain things, such as preventive maintenance, operation to specification, perhaps even guaranteed maximum down-time. The one-off customer has none of this. The service organization is contractually bound to the contract customer. Period. Yes, the one off cash is nice, but the regular customers are far more valuable in the long run. They often are happier customer, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 13, 2011 8:59 AM To: kenconverse-at-qualityimages.biz
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 } 23, 46 -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe .ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 } 23, 46 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 46 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 } 23, 46 -- Subject: RE: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Topic: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB } 23, 46 -- Content-Language: en-US } 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB } 23, 46 -- Content-Type: text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit } 23, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date: X-from:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References: In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm 2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO5XVi jSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Fri May 13 08:22:05 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DDM5G5001569 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:22:05 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=2lq4+qobG88tJFkiXmxSLStFAwyj+PriBwHB+7DDebs= c=1 sm=0 a=d6ZFszS330cA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=DahTZl4gAAAA:8 a=Zx37jsudAAAA:8 a=_CgzANL9AAAA:8 a=-gyhParZAAAA:8 a=hCCmtxs2AAAA:8 a=yMhMjlubAAAA:8 a=CjxXgO3LAAAA:8 a=15LFL-canmR1PGpTlQ8A:9 a=YOHmFmhgio4DZwt2t8EA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=U5vkcOzocHIA:10 a=brlFRoIUItEA:10 a=RO2JPw24U6UA:10 a=PmYly_lw2igA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=f96-IYUahzkA:10 a=G91thX30KR8A:10 a=rC2wZJ5BpNYA:10 a=yUsKKQj0Tui2F24u:21 a=NZ21XijlkcsDGYSt:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2310] helo=Ken) 20, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id AC/77-17273-C703DCD4; Fri, 13 May 2011 13:22:05 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {vray-at-partbeamsystech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 20, 28 -- Date: Fri, 13 May 2011 09:21:53 -0400 20, 28 -- Message-ID: {784D2C0EDDA74302903AA04B2F8E61BF-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- Importance: Normal 20, 28 -- Thread-Index: AcwRbZETKQmlIEYgQWGwqB32vBi+0AAAjrwg 20, 28 -- In-Reply-To: {201105131259.p4DCxF4C021152-at-ns.microscopy.com} 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DDM5G5001569 ==============================End of - Headers==============================
It is my understanding, purely hearsay, you understand, that NIST and NIH have dedicated vendor service engineers attending some installations of individual EM's.
Forgive the thoughts from the mote to the asteroid, but the notion of planning for/against the TOTAL COST OF OWNERSHIP is not new and is likely taught in all credible schools of business. Whether anyone hires individuals with such expertise is debatable.
We, for example just took receipt of a small XRD whose fundamental OS is apparently a vendor-specific linux on which an embedded version of XP runs the windows applications. There must be a reason for manufacturers to finally resort to this reasonable tactic in production and service. This one anyway must see the advantage to be in control of all of its product.
To seek efficiency in service, one probably should seek less diversity in source of large scientific instrumentation. If all electron microscopes come from one vendor - say, ten instruments - it might be more efficient to pay for one resident service engineer - trained and paid by the vendor, than to pay for one engineer to service a diversity within ten and not to have vendor-level of access to all of the vendor-specific hardware parts. One gets more well aligned instruments working at peak efficiency.
If the vendor must supply - a la carte - the control PC with its vendor-manufactured controlling boards, and does so at a price intended to convince the user of the advisability of purchasing a service contract, then any purchaser that doesn't understand the TCO concept has little chance of successfully managing down the total cost of ongoing service. If 5-10% of cost = annual cost of service, then any reasonable plan should likely conclude that a decade of use will mark the end of serviceability of even the most expensive microscope. i.e., If one has not found a reason (or wish) to upgrade an instrument in a decade, then one will have found a good reason to keep it running for another decade (in our historic view, that means running the control software today on a year 2000 PC with severe limitations on video, memory, CPU, etc. What are the prospects for a system that still runs on NT4 today, and will anyone agree that with such an instrument and no other prospects, the need for a service contract on such an instrument is more valuable than when it was new!
New prospect: If instrument leasing carried an advantage, then the lessor also would be paid for carrying the burden of service. TCO becomes something more manageable such as Total Cost of USE (TCU). The lessor and its customers determine the level of instrument 'quality' that is required, the Duration of Service (DOS) estimated by the lessee as well as the user base on which the lease is based. Lessors might even become regional providers in fixed locations for institutional users with 'spotty' requirements for use and associated services.
Finally, what would any reader of this message say to a donor who came bearing a FEI Titan as a gift? I think I know the question I would ask from the location of my office on the base of the pyramid before I could muster the courage to speak to my supervisor about the offer.
Cheers, and good luck to us all.
Fred Monson
Planning for the future is all well and good, but it is only based on what one knows of the past.
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
-----Original Message----- X-from: WHITTAKS-at-si.edu [mailto:WHITTAKS-at-si.edu] Sent: Thursday, May 12, 2011 2:31 PM To: Monson, Frederick
Ironically we just last week were informed of an exploratory committee being set up to address the rather substantial service contract costs the Institution pays annually with tax dollars drying up-and Jonathan- you were tops of my list to call on. One of the measures under exploration is the hiring of individuals to provide service onsite. I have not heard of a budget academic type EM facility that has made this model cost effective but would be interested in discussing this with anyone out there who has.
Just examining EM's, my Lab has 4 basic units from 3 different manufacturers all under continuous contract and if we include the rest of the machines in very close proximity we have 7 basic from 4. And the whole place 10 from 5. I would love to hear from anyone who is making onsite service in the absence of a contract work; in an academic type setting; with instruments from multiple manufacturers driven by relatively inexperienced users.
Thanks for any input,
Scott Whittaker Chief NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
The SEM Lab move is complete and is now located in room EG13B. East Wing Ground floor.
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Thursday, May 12, 2011 12:49 PM To: Whittaker, Scott
Friends:
We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training.
As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments.
Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic.
We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts.
This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not?
Hoping for a miracle,
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 13, 34 -- May 2011 09:47:24 -0700 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 34 -- Content-Type: text/plain; charset=us-ascii 13, 34 -- Subject: Microscope service strategies 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 13, 34 -- X-Mailer: Apple Mail (2.1084) 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 29 -- From WHITTAKS-at-si.edu Thu May 12 13:24:53 2011 27, 29 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 27, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4CIOrt3001983 27, 29 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2011 13:24:53 -0500 27, 29 -- Received: from SI-MSEHUB-N01.US.SINET.SI.EDU (si-msehubi-01.us.sinet.si.edu [160.111.49.142]) 27, 29 -- by si-mailout04.si.edu (Postfix) with ESMTP id 2F7BC5D70; 27, 29 -- Thu, 12 May 2011 14:24:53 -0400 (EDT) 27, 29 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::58ac:1cff:44b0:524e]) by 27, 29 -- SI-MSEHUB-N01.US.SINET.SI.EDU ([fe80::34ec:9394:533a:63ad%11]) with mapi; 27, 29 -- Thu, 12 May 2011 14:24:52 -0400 27, 29 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 27, 29 -- To: "'jkrupp-at-deltacollege.edu'" {jkrupp-at-deltacollege.edu} 27, 29 -- CC: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 27, 29 -- Date: Thu, 12 May 2011 14:24:51 -0400 27, 29 -- Subject: RE: [Microscopy] Microscope service strategies 27, 29 -- Thread-Topic: [Microscopy] Microscope service strategies 27, 29 -- Thread-Index: AcwQxHPf8MiJ1t2HT9yLD8R7S5j7OwABiSOQ 27, 29 -- Message-ID: {038C04650A93A14381E944A473695F619399F10ACC-at-SI-MSEV03.US.SINET.SI.EDU} 27, 29 -- References: {201105121648.p4CGmfGc019233-at-ns.microscopy.com} 27, 29 -- In-Reply-To: {201105121648.p4CGmfGc019233-at-ns.microscopy.com} 27, 29 -- Accept-Language: en-US 27, 29 -- Content-Language: en-US 27, 29 -- X-MS-Has-Attach: 27, 29 -- X-MS-TNEF-Correlator: 27, 29 -- acceptlanguage: en-US 27, 29 -- Content-Type: text/plain; charset="iso-8859-1" 27, 29 -- MIME-Version: 1.0 27, 29 -- Content-Transfer-Encoding: 8bit 27, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CIOrt3001983 ==============================End of - Headers==============================
==============================Original Headers============================== 47, 36 -- From FMonson-at-wcupa.edu Fri May 13 10:44:56 2011 47, 36 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) 47, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DFiuT1019878 47, 36 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 10:44:56 -0500 47, 36 -- X-ASG-Debug-ID: 1305301495-0368bc13a41f91d0001-4CH8be 47, 36 -- Received: from WCU-XCH-01.PASSHE.LCL (wcu-xch-01.wcupa.edu [144.26.0.85]) by MX01.WCUPA.EDU with ESMTP id KAoDaUWg6dQWEXVn (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 11:44:55 -0400 (EDT) 47, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 47, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.85 47, 36 -- X-ASG-Whitelist: Client 47, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 47, 36 -- WCU-XCH-01.PASSHE.LCL ([fe80::d594:aa4c:f7e5:2e16%16]) with mapi id 47, 36 -- 14.01.0270.001; Fri, 13 May 2011 11:44:55 -0400 47, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 47, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 47, 36 -- Subject: RE: [Microscopy] RE: Microscope service strategies 47, 36 -- Thread-Topic: [Microscopy] RE: Microscope service strategies 47, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] RE: Microscope service strategies 47, 36 -- Thread-Index: AQHMENLMQ1B07+B1j0mc4BcQ5CiXwJSK3biw 47, 36 -- Date: Fri, 13 May 2011 15:44:54 +0000 47, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C05ED8F-at-WCU-XCH-03.PASSHE.LCL} 47, 36 -- References: {201105121831.p4CIVNCN014500-at-ns.microscopy.com} 47, 36 -- In-Reply-To: {201105121831.p4CIVNCN014500-at-ns.microscopy.com} 47, 36 -- Accept-Language: en-US 47, 36 -- Content-Language: en-US 47, 36 -- X-MS-Has-Attach: 47, 36 -- X-MS-TNEF-Correlator: 47, 36 -- x-originating-ip: [10.28.57.79] 47, 36 -- Content-Type: text/plain; charset="iso-8859-1" 47, 36 -- MIME-Version: 1.0 47, 36 -- X-Barracuda-Connect: wcu-xch-01.wcupa.edu[144.26.0.85] 47, 36 -- X-Barracuda-Start-Time: 1305301495 47, 36 -- X-Barracuda-Encrypted: AES128-SHA 47, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 47, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 47, 36 -- Content-Transfer-Encoding: 8bit 47, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DFiuT1019878 ==============================End of - Headers==============================
A colleague at our institution is setting up his donated FE SEM and EDS system and needs an instruction manual.
Specifically, he has an Oxford Instruments EDS detector linked to Link XP3 pulse processor (TMXP3) working in conjunction with 4PI board and DTSA software.
So, he needs a technical manual describing how to set up the detector with the Link XP3 controller. Any help would be appreciated.
Thank you
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 18 -- From bozzola-at-siu.edu Fri May 13 10:53:28 2011 6, 18 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DFrR6l000613 6, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 10:53:28 -0500 6, 18 -- Received: by iwg8 with SMTP id 8so3260597iwg.0 6, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:53:27 -0700 (PDT) 6, 18 -- MIME-Version: 1.0 6, 18 -- Received: by 10.42.150.135 with SMTP id a7mr1950875icw.316.1305302007761; Fri, 6, 18 -- 13 May 2011 08:53:27 -0700 (PDT) 6, 18 -- Received: by 10.42.180.133 with HTTP; Fri, 13 May 2011 08:53:27 -0700 (PDT) 6, 18 -- Date: Fri, 13 May 2011 10:53:27 -0500 6, 18 -- Message-ID: {BANLkTikr=cskRBeKjumBJRuAZO4H0k1F4A-at-mail.gmail.com} 6, 18 -- Subject: EDS: Oxford Link Setup 6, 18 -- From: John Bozzola {bozzola-at-siu.edu} 6, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 18 -- Content-Type: text/plain; charset=UTF-8 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DFrR6l000613 ==============================End of - Headers==============================
I am at NIH in Bethesda, MD and work with two TEMs from JEOL and a Hitachi SEM in our EM Core Facility. I believe that I can speak to the beginning of Fred's remarks. Our vendor service engineers work at different locations although as is the case with most companies that I have been familiar with, they tend to send the closest man and the ones who are most familiar with my scopes.
There are many scopes from several different companies on campus so it may seem that one person is dedicated to work on our scopes but this is not true as far as I know.
Pat Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Dear List, I have about a dozen CBED patterns obtained from the same specimen at different orientations, and I need to determine the zone axes and reflections. The specimen is too thick to use the method of constructing Kikuchi maps outlined in Williams and Carter. For many of the patterns I was able to find a reflection in the [110] pattern that is the same in the unknown pattern, and I could simulate various possibilities for the pole of the unknown pattern and compare these to the observed pattern. However, there are still a few patterns that are ambiguous, and for my best guesses the spot in the [110] that is expected to match is very high order and doesn't appear in either pattern. Can anyone point me to a better reference than Williams and Carter (if such exists), or give me other words of wisdom? TIA. Yours, Bill
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both drg.mitchell-at-sydney.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: drg.mitchell-at-sydney.edu.au Name: David Mitchell
Organization: ACMM/University of Sydney
Title-Subject: [Filtered] TEM: Large UPS systems - stray fields?
Message: Dear Listers
I am considering installing an inverter type UPS to keep my FEG TEM running during power outages/blips and to provide it with clean power. My options for locating the UPS and battery stack are limited. I obviously want it located as far from the microscope as possible and plan on putting it in the plant room for the microscope, adjacent to the lens/ion pump power supplies. Does anyone have any experience with, or advice on, potential interference issues between UPSs and TEM systems?
Thanks and regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, TEM Manager
Contact: PH +61 2 9036 7633 FAX +61 2 9351 7682 drg.mitchell-at-sydney.edu.au Address: Australian Centre for Microscopy & Microanalysis Incorporating: Australian Microscopy & Microanalysis Research Facility ARC Centre of Excellence for Design in Light Metals Madsen Building F09, Room 128A The University of Sydney NSW, 2006, Australia sydney.edu.au/acmm {http://www.sydney.edu.au/acmm} www.ammrf.org.au {http://www.ammrf.org.au/} www.dmscripting.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball Institute of Biomolecular Medicine
Title-Subject: [Filtered] service contracts
Message: Universities make money from grants, but research is a low margin business, and thus the pressure on service contract expenses. The service contracts on the clinical instruments are not under such pressure; being down for even hours can seriously cut into billings.
Getting grants requires steady publications and publications require reliable high tech equipment. (Novelty sells.) Therefore, service contracts are a necessary capital expense. Administrators need to understand that to protect both the brand of the university as cutting edge and to raise capital, the expense of maintenance fees is essential. This is a simple business decision.
Dear Dave, The UPS attached to the FEI Polara FEG TEM at Caltech was located in an adjacent room, ~10 m from the scope. The Haskris coolers, air compressors, and some of the electronics were also in this room. We saw no interference from the UPS. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
Email: drg.mitchell-at-sydney.edu.au Name: David Mitchell
Organization: ACMM/University of Sydney
Title-Subject: [Filtered] TEM: Large UPS systems - stray fields?
Message: Dear Listers
I am considering installing an inverter type UPS to keep my FEG TEM running during power outages/blips and to provide it with clean power. My options for locating the UPS and battery stack are limited. I obviously want it located as far from the microscope as possible and plan on putting it in the plant room for the microscope, adjacent to the lens/ion pump power supplies. Does anyone have any experience with, or advice on, potential interference issues between UPSs and TEM systems?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DNepPJ008443 for {MicroscopyListserverArchive2-at-microscopy.com} ; Fri, 13 May 2011 18:40:51 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p4DNepxm008441; Fri, 13 May 2011 18:40:51 -0500
The Phase One UPS I had on my 2010F at Intel had a requirement from JEOL that it be at least 20 feet away from the microscope. I measured 35 milligauss coming out of that thing. We kept it 50 feet from the microscope. The new lab at ASU has the UPS located in the attic at the far end of the building, and there is extensive shielding between the UPS and the microscopes. You definitely want the UPS as far from your column as possible.
A. John Mardinly, ASU
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, May 13, 2011 3:42 PM To: John Mardinly
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both drg.mitchell-at-sydney.edu.au as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: drg.mitchell-at-sydney.edu.au Name: David Mitchell
Organization: ACMM/University of Sydney
Title-Subject: [Filtered] TEM: Large UPS systems - stray fields?
Message: Dear Listers
I am considering installing an inverter type UPS to keep my FEG TEM running during power outages/blips and to provide it with clean power. My options for locating the UPS and battery stack are limited. I obviously want it located as far from the microscope as possible and plan on putting it in the plant room for the microscope, adjacent to the
lens/ion pump power supplies. Does anyone have any experience with, or advice on, potential interference issues between UPSs and TEM systems?
Thanks and regards,
Dave Mitchell
Dr David Mitchell Senior Microscopist, TEM Manager
Contact: PH +61 2 9036 7633 FAX +61 2 9351 7682 drg.mitchell-at-sydney.edu.au Address: Australian Centre for Microscopy & Microanalysis Incorporating: Australian Microscopy & Microanalysis Research Facility ARC Centre of Excellence for Design in Light Metals Madsen Building F09, Room 128A The University of Sydney NSW, 2006, Australia sydney.edu.au/acmm {http://www.sydney.edu.au/acmm} www.ammrf.org.au {http://www.ammrf.org.au/} www.dmscripting.com
The fields fall off with the distance squared. I would put it as far away as reasonably possible, but I would not get too heroic about it unless it would putting it any closer would put you out of spec. I would think the UPS manufacturers are aware that some of their units will be used with sensitive equipment and they would offer a well shielded system. Of course they may charge extra for that shielding.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, May 13, 2011 5:30 PM To: wesaia-at-iastate.edu
Does anyone have a protocol for going from straight-from-the-store yogurt to prepared SEM specimen? I have a student interested in seeing what the bacteria looks like. They are also trying to find out just how much of yogurt is, in fact, bacteria. Not being a biologist, I'm at a bit of a loss...
--Justin A. Kraft
==============================Original Headers============================== 2, 37 -- From kraftpiano-at-gmail.com Sat May 14 17:20:11 2011 2, 37 -- Received: from mail-gx0-f169.google.com (mail-gx0-f169.google.com [209.85.161.169]) 2, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4EMKBdR024280 2, 37 -- for {microscopy-at-microscopy.com} ; Sat, 14 May 2011 17:20:11 -0500 2, 37 -- Received: by gxk23 with SMTP id 23so1499807gxk.0 2, 37 -- for {microscopy-at-microscopy.com} ; Sat, 14 May 2011 15:20:11 -0700 (PDT) 2, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 37 -- d=gmail.com; s=gamma; 2, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 2, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 2, 37 -- bh=vLo2EgGwMjLnVmRdzTUAO2MyewRK4TY57AlbA6sdyPc=; 2, 37 -- b=Y11zFMYCKzxmlSK1G2BR5MYk8s2lGt8g90NyXT6KbU2TGXjYORE5ZYyxMTgXnk3rWu 2, 37 -- RFGqk9i6ALM9dMQFTwXsGasupDjunSGLbUDYXzmdTAy0b0Ye/5GJnjY5TmoiKgU4D+aY 2, 37 -- ZzfYdF8aw8fwojy3Iql7a8UGxWPPfFj1zpwGc= 2, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 2, 37 -- d=gmail.com; s=gamma; 2, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 2, 37 -- :to:mime-version:x-mailer; 2, 37 -- b=kq9xo4nOHGceYVNkT1DxsBuRoS+lJIarJYKcuHf7PUY8+udIWy/1cvlekZIapYt4o0 2, 37 -- +4xmgPna4lQtBW2RqomH3jtJ/rbVDChiMS+ONg4+EvygHEDqWzfSPZs+GqGhP0V1LU97 2, 37 -- dt54lM+eXnTqGpgQZOHh91j9CWYqYcDOc58OA= 2, 37 -- Received: by 10.236.78.200 with SMTP id g48mr2888885yhe.465.1305411611498; 2, 37 -- Sat, 14 May 2011 15:20:11 -0700 (PDT) 2, 37 -- Received: from [10.101.1.3] (c-98-249-167-116.hsd1.fl.comcast.net [98.249.167.116]) 2, 37 -- by mx.google.com with ESMTPS id 41sm1748035yha.65.2011.05.14.15.20.09 2, 37 -- (version=TLSv1/SSLv3 cipher=OTHER); 2, 37 -- Sat, 14 May 2011 15:20:10 -0700 (PDT) 2, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 2, 37 -- Content-Type: text/plain; charset=us-ascii 2, 37 -- Subject: SEM preparation of yogurt bacteria? 2, 37 -- Date: Sat, 14 May 2011 18:20:07 -0400 2, 37 -- Message-Id: {176D8FD7-59EF-409E-AD66-629A37AA6EB3-at-gmail.com} 2, 37 -- To: microscopy-at-microscopy.com 2, 37 -- Mime-Version: 1.0 (Apple Message framework v1084) 2, 37 -- X-Mailer: Apple Mail (2.1084) 2, 37 -- Content-Transfer-Encoding: 8bit 2, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4EMKBdR024280 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjwanzek-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mjwanzek-at-gmail.com Name: Mike
Organization: Washington State University
Title-Subject: [Filtered] JEM 1200EX
Message: I purchased a JEM 1200EX TEM microscope from a university surplus auction. I don't know much about electron microscopes, and I listed it on Ebay for $5000 (just go to ebay and search "jem 1200ex").
Anyways, I got a quesion asking if it has a "high angle detector". I don't know what this is, and I couldn't find out from some googling, so does anyone know what this is, where it is located, and what it looks like?
Also, who would you expect would be interested in an auction like this? Do you think universities or companies, would be interested, or mainly people who just want an electron microscope that they can set up in their house? Thanks. Login Host: 134.121.117.20 ---------------------------------------------------------------------------
==============================Original Headers============================== 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun May 15 15:51:54 2011 9, 25 -- Received: from znl.com ([206.69.208.20]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4FKpsqb009380 9, 25 -- for {microscopy-at-microscopy.com} ; Sun, 15 May 2011 15:51:54 -0500 9, 25 -- Received: from localhost (localhost [127.0.0.1]) 9, 25 -- by znl.com (Postfix) with ESMTP id 4935E39977D 9, 25 -- for {microscopy-at-microscopy.com} ; Sun, 15 May 2011 15:51:54 -0500 (CDT) 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 25 -- Received: from znl.com ([127.0.0.1]) 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 25 -- with ESMTP id 6Of-nBuDbSq0 for {microscopy-at-microscopy.com} ; 9, 25 -- Sun, 15 May 2011 15:51:52 -0500 (CDT) 9, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 9, 25 -- by znl.com (Postfix) with ESMTPA id 9791E399772 9, 25 -- for {microscopy-at-microscopy.com} ; Sun, 15 May 2011 15:51:52 -0500 (CDT) 9, 25 -- Message-ID: {4DD03CE8.6050706-at-microscopy.com} 9, 25 -- Date: Sun, 15 May 2011 15:51:52 -0500 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 9, 25 -- Reply-To: mjwanzek-at-gmail.com 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 25 -- MIME-Version: 1.0 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 25 -- Subject: viaWWW:JEM 1200EX 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Basically what you are saying is that people with more money are happier. This is quite an evidence, isn't it? I don't think people leave a service contract when they don't have to.
Regards,
Stephane
----- Original Message ---- X-from: "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} To: nizets2-at-yahoo.com Sent: Fri, May 13, 2011 3:25:39 PM
Valery, Very well stated. The only thing I might add is that the customer with a service contract is guaranteed certain things, such as preventive maintenance, operation to specification, perhaps even guaranteed maximum down-time. The one-off customer has none of this. The service organization is contractually bound to the contract customer. Period. Yes, the one off cash is nice, but the regular customers are far more valuable in the long run. They often are happier customer, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 13, 2011 8:59 AM To: kenconverse-at-qualityimages.biz
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 } 23, 46 -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe .ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 } 23, 46 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 46 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 } 23, 46 -- Subject: RE: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Topic: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB } 23, 46 -- Content-Language: en-US } 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB } 23, 46 -- Content-Type: text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit } 23, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date: X-from:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References: In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm 2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO5XVi jSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Fri May 13 08:22:05 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DDM5G5001569 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:22:05 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=2lq4+qobG88tJFkiXmxSLStFAwyj+PriBwHB+7DDebs= c=1 sm=0 a=d6ZFszS330cA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=DahTZl4gAAAA:8 a=Zx37jsudAAAA:8 a=_CgzANL9AAAA:8 a=-gyhParZAAAA:8 a=hCCmtxs2AAAA:8 a=yMhMjlubAAAA:8 a=CjxXgO3LAAAA:8 a=15LFL-canmR1PGpTlQ8A:9 a=YOHmFmhgio4DZwt2t8EA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=U5vkcOzocHIA:10 a=brlFRoIUItEA:10 a=RO2JPw24U6UA:10 a=PmYly_lw2igA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=f96-IYUahzkA:10 a=G91thX30KR8A:10 a=rC2wZJ5BpNYA:10 a=yUsKKQj0Tui2F24u:21 a=NZ21XijlkcsDGYSt:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2310] helo=Ken) 20, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id AC/77-17273-C703DCD4; Fri, 13 May 2011 13:22:05 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {vray-at-partbeamsystech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 20, 28 -- Date: Fri, 13 May 2011 09:21:53 -0400 20, 28 -- Message-ID: {784D2C0EDDA74302903AA04B2F8E61BF-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- Importance: Normal 20, 28 -- Thread-Index: AcwRbZETKQmlIEYgQWGwqB32vBi+0AAAjrwg 20, 28 -- In-Reply-To: {201105131259.p4DCxF4C021152-at-ns.microscopy.com} 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DDM5G5001569 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 50 -- From nizets2-at-yahoo.com Mon May 16 03:39:53 2011 35, 50 -- Received: from nm14.bullet.mail.sp2.yahoo.com (nm14.bullet.mail.sp2.yahoo.com [98.139.91.84]) 35, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4G8dqB9001730 35, 50 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 03:39:52 -0500 35, 50 -- Received: from [98.139.91.67] by nm14.bullet.mail.sp2.yahoo.com with NNFMP; 16 May 2011 08:39:52 -0000 35, 50 -- Received: from [98.139.91.42] by tm7.bullet.mail.sp2.yahoo.com with NNFMP; 16 May 2011 08:39:52 -0000 35, 50 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 16 May 2011 08:39:51 -0000 35, 50 -- X-Yahoo-Newman-Property: ymail-3 35, 50 -- X-Yahoo-Newman-Id: 985366.66804.bm-at-omp1042.mail.sp2.yahoo.com 35, 50 -- Received: (qmail 88670 invoked by uid 60001); 16 May 2011 08:39:51 -0000 35, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305535191; bh=fZxUvAQ9uanwFcclKg1xLte2Y46aBpLDOTew1DueBuA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=D9OY+i7Yy06WK8ye3kbgOVawYeCG3X4SmrvTh82j8DuTh4ge3VRt3cwHk/pWUD+DnyhEgOGYYQEThtpuGUMdx7xrbGaKTahd73BgLfgG7IUBbMzDw2wP86zn9ITs30GkmPKya3lmH4huCHexfRAtqkFi3PgJEFfzOpxTMhGtFvo= 35, 50 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 35, 50 -- s=s1024; d=yahoo.com; 35, 50 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 35, 50 -- b=yI/vGamFIYuqC8MCqdC+O0r+kzSLAigv8n+TCTofFh7Ga9OoydBMjXXMR/yr2aBAboyKd36vCFqPAGFVvGN/R7tNJHqDYrTsPqlQJZ9brd6kTI3HEhhOlcXH9Plk5UKnip7CWTYxUiOXiQbpb07dEkIAzTg+wwrRIAT5sjm8DYk=; 35, 50 -- Message-ID: {670062.87435.qm-at-web110801.mail.gq1.yahoo.com} 35, 50 -- X-YMail-OSG: Cqu_fvMVM1mop0TJob1Pp_Izp2tPkPfirUrRne90yLdA0VK 35, 50 -- ve3pAEiziidnyPImy3RC3JWto.8Oz9Zy2Hncumgx9l_qC35fMlLmaimIV9qm 35, 50 -- jfnBUR0JCTBo_xwq8eTLVylTHy9MyQl6CyGYNtJCUq57iTIrqd1ziMsMMK7P 35, 50 -- nCTCtGvgTg5aiDySlBlc2AbuhwpnoIspW56ApH_59ZGacohTqNdeEZkwTGl4 35, 50 -- nGzzFkK5Bk.WMvy_51uzPGOTrcSntrfbyYs40OqKZ4pRMlWNIYIKhDqC_V76 35, 50 -- L0WbDCuGvRIWWX2U7n4odTALrl1AWU7fBLj_O732r_FNAE79YcVdHwrgGI4t 35, 50 -- .wzpkvdmKcJdqeTmsnICB01O7TXeajgktQ_qnBS1JUOWyrquPu7PlL3vRaRI 35, 50 -- YYiuS_yy2zZYRw0aG8Skleflb8C0AGNNVYi_eiDl7X2QStZH9TYCT5xoqwNm 35, 50 -- 7.xi8Z9S2CYwngkGYXrqnvDSS1PxJvu1NKQ8amGcMgrs3zfxkOMjnFd9Wq_m 35, 50 -- pWGrwINTsOnwsw1omHWplvWjWfl0CfLqTSOoUPHWzt..d1HzX54K_QYNOj6P 35, 50 -- ZHKWvTqOJB3lRMiZ45R62Z6Zeb1P1E3ItsfC8Kyv0kxHnijWZ1_8JeflEeJ8 35, 50 -- aYuO7uxTdebY89Nr1W6rXObX0WIQtCt3vr6yKgOcRxBR3kfo1MjnAEu2Z6e4 35, 50 -- zg_epvUT3MCZzwiQg1.XcCGC5baYqVl0zr19u1SoPiGIIpOMLjXChvhSTJWq 35, 50 -- v3zoh4Gu.lQGxpVueBS8QrsH_V9E.pIE5vvFWo6quVID3G9Xsn8dHzFxTb8G 35, 50 -- UYfgAS1Q15iZNjrCISUircGbdjX3VxgriWSpuo7Z2XHhBJP8hcEhV9jUEN2x 35, 50 -- _vAGqh2jeL0JLB6ZDS8lyv5BhXSdpj2nTNvkOOLMhqfUll6EFf1Po6i_kVHk 35, 50 -- IYTyj0snuIY.et9HuWRBUz3x9Mv4c38PC.CIO6624VwVKICUcHP9JNVEMrSe 35, 50 -- 8EsUoJ94wyRuKeLBQDgWqLN677IDuPQr_Agg6pZpGzTnKLdAtme2DjG0Vqk2 35, 50 -- Riaa9KKV3SEbB0raZEBmmh_8hGk0cJItRwsOeUWdNDCk7N0QN0Dp_XyfA0jp 35, 50 -- Vau._ALv2dlszc9JDu4z20IMOhvZGyY5A1bAJBqO.T6zIuL3VVNS8Wpo6Aid 35, 50 -- K5BNeA9I.L3tPX8cz2MMrgwCGa5JOdnG95N7ebgzojtXyFEOmosj_mxyROcV 35, 50 -- QWG6TWRgzrWagjf9eCBqXVIlvuEwCXgcerYfpJxCEuyiQORkXdQJOSLxL 35, 50 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Mon, 16 May 2011 01:39:51 PDT 35, 50 -- X-Mailer: YahooMailRC/567 YahooMailWebService/0.8.111.303096 35, 50 -- References: {201105131325.p4DDPdpV007301-at-ns.microscopy.com} 35, 50 -- Date: Mon, 16 May 2011 01:39:51 -0700 (PDT) 35, 50 -- From: Stephane Nizet {nizets2-at-yahoo.com} 35, 50 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 35, 50 -- To: microscopy-at-microscopy.com 35, 50 -- In-Reply-To: {201105131325.p4DDPdpV007301-at-ns.microscopy.com} 35, 50 -- MIME-Version: 1.0 35, 50 -- Content-Type: text/plain; charset=iso-8859-1 35, 50 -- Content-Transfer-Encoding: 8bit 35, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4G8dqB9001730 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cserhati-at-delfin.unideb.hu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Dear Collegaues, I am looking for film casettes for JEOL TEM for the following film size: 99.6 x 80.9 mm (4"x3 1/4"). It would be nice if there were some second-hand ones.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both degraef-at-cmu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: degraef-at-cmu.edu Name: Marc De Graef
Organization: Carnegie Mellon University
Title-Subject: [Filtered] Remote and in-situ microscopy workshop
Message: Dear colleague,
On June 6-8, 2011, the Third International Workshop on Remote Electron Microscopy and In-Situ Studies will take place at Carnegie Mellon University, Pittsburgh, PA. On behalf of my co-organizers, I would like to invite you to consider attending this workshop. We have a truly outstanding group of invited speakers:
Amanda Petford-Long (ANL), Angus Wilkinson (Oxford), Eric Stach (BNL), Angus Kirkland (Oxford), Peter Crozier (ASU), Katayun Barmak (CMU), Robert Sinclair (Stanford), Judith Yang (U. Pittsburgh), John Mansfield (U. Michigan), Alejandro Zúñiga (U. Chile), Paulo Ferreira (U. Texas), Michael Uchic (WPAFB), Prakash Palanisamy (U. Virginia), Phil Batson (Rutgers U.), Eva Olsson (Chalmers U.), Uli Dahmen (LBL), Frans Tichelaar (Delft U. Tech.), Ian Robertson (U. Illinois), Vicente Garibay (U. Mexico), Andrew Minor (UCBerkeley), Jan Rignalda (FEI), Steven Mick (ProtoChips), Pushkarraj Deshmukh (Fishione), ...
and the Plenary Talk will be given by Dr. Peter Swann.
Information on the workshop, including the scientific program, can be found at the following URL:
http://mpg.web.cmu.edu/CMU-IWREMISS-2011.html
[please note that the presentation abstract will pop up when you hover the mouse over the letter A that precedes each presentation title.]
Registration is free (first-come, first-serve), and hotel and travel information can be found on the web site.
We are looking forward to seeing you at the workshop in June!
With kind regards,
Marc De Graef Tom Nuhfer Robert Sinclair Richard Chin
Stephane, No, what I'm saying is that some people are happier because of HOW they spend their money.
To start with some of the more obvious places:
If the system is down because service is not readily available then A) your students and researchers are not able to do what is required/needed in a timely manner, B) you need to send your work out to another lab and pay by the hour to use their systems or, C) your customers take their work elsewhere (to a competitor) to be done on a timely basis. Even the bean counters can see some of the costs involved here, although they are likely to "overlook" at least some of them.
There are some less obvious costs, also:
A) You bought a system with 3.5nm resolution but through many little problems (death through a thousand tiny cuts) that have accumulated over time, it will only produce 20nm resolution. First, you may not be able to do the work that needs to be done, resulting in similar expenses to above. Second, if your response is, "I don't need better than 20nm resolution for my work" then a considerable amount of money was wasted in the original purchase. B) Productivity goes down because the people using the system (whether "tool operator" or "microscopist") know it doesn't operate properly and become discouraged.
The people with the service contract are happier because they don't have to constantly worry about their system. They are confident that it will remain operating up to spec. The people without the service contract may actually be spending as much money, if not more, but the amounts aren't always obvious. In addition, they have to constantly worry whether or not their system is going to be able to do the work that needs to be done. That's why they are less likely to be happy.
The benefits of service contracts are not entirely obvious and this shows very clearly in scientific grants. It's not all that difficult to get someone to give a lot of money for a building that will have their name on it. It may be even easier to get someone to give money for a fancy state of the art piece of equipment that will have a bronze plaque on it. There are no bronze plaques adorning service contracts, hence grant money for such unseen things is more difficult to come by. Is it of less importance? I have my opinion on that. Others have different opinions.
X-from your many posts, it appears that you have the necessary skills (and time) to keep your system running to spec. Congratulations! In my experience you are in the minority. I admire people who really get deeply into their equipment (I'm like them), but others get deeply into other aspects of their jobs and view the equipment as a fancy hammer or screwdriver that they know how to use, but not necessarily repair or maintain. They are not better or worse, just different (as my kids were told before going on Rotary Youth Exchange). There are many things that I don't repair because someone else has more expertise, the tools and the parts to do it more efficiently than I can.
It's about HOW you spend your money, not that you have more.
Sincerely, Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Monday, May 16, 2011 4:43 AM To: kenconverse-at-qualityimages.biz
Basically what you are saying is that people with more money are happier. This is quite an evidence, isn't it? I don't think people leave a service contract when they don't have to.
Regards,
Stephane
----- Original Message ---- X-from: "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} To: nizets2-at-yahoo.com Sent: Fri, May 13, 2011 3:25:39 PM
Valery, Very well stated. The only thing I might add is that the customer with a service contract is guaranteed certain things, such as preventive maintenance, operation to specification, perhaps even guaranteed maximum down-time. The one-off customer has none of this. The service organization is contractually bound to the contract customer. Period. Yes, the one off cash is nice, but the regular customers are far more valuable in the long run. They often are happier customer, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 13, 2011 8:59 AM To: kenconverse-at-qualityimages.biz
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 } 23, 46 -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe .ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 } 23, 46 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 46 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 } 23, 46 -- Subject: RE: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Topic: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB } 23, 46 -- Content-Language: en-US } 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB } 23, 46 -- Content-Type: text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit } 23, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date: X-from:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:Reference s: In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm 2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO5XVi jSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Fri May 13 08:22:05 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DDM5G5001569 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:22:05 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=2lq4+qobG88tJFkiXmxSLStFAwyj+PriBwHB+7DDebs= c=1 sm=0 a=d6ZFszS330cA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=DahTZl4gAAAA:8 a=Zx37jsudAAAA:8 a=_CgzANL9AAAA:8 a=-gyhParZAAAA:8 a=hCCmtxs2AAAA:8 a=yMhMjlubAAAA:8 a=CjxXgO3LAAAA:8 a=15LFL-canmR1PGpTlQ8A:9 a=YOHmFmhgio4DZwt2t8EA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=U5vkcOzocHIA:10 a=brlFRoIUItEA:10 a=RO2JPw24U6UA:10 a=PmYly_lw2igA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=f96-IYUahzkA:10 a=G91thX30KR8A:10 a=rC2wZJ5BpNYA:10 a=yUsKKQj0Tui2F24u:21 a=NZ21XijlkcsDGYSt:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2310] helo=Ken) 20, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id AC/77-17273-C703DCD4; Fri, 13 May 2011 13:22:05 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {vray-at-partbeamsystech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 20, 28 -- Date: Fri, 13 May 2011 09:21:53 -0400 20, 28 -- Message-ID: {784D2C0EDDA74302903AA04B2F8E61BF-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- Importance: Normal 20, 28 -- Thread-Index: AcwRbZETKQmlIEYgQWGwqB32vBi+0AAAjrwg 20, 28 -- In-Reply-To: {201105131259.p4DCxF4C021152-at-ns.microscopy.com} 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DDM5G5001569 ==============================End of - Headers==============================
A SiPin detector is being used on a vacuum chamber equipped with a cold cathode discharge electron gun. Occasionally there are "cut-outs".Apparently some of the electrical noise in the cold cathode discharge is being picked up by the detector and it is sufficient to trigger it off.
All the usual things like rearranging grounds, running with surge suppressors, running the computer from the battery only, etc., have been tried and make very little difference. We have even used a separately powered USB Hub with no improvement. What is notable is that with an older Dell Inspiron the problem is much less than with a brand new Dell Inspiron.
Any suggestions would be welcomed
-- Travis Johnson Bedford MA
==============================Original Headers============================== 4, 30 -- From travisj25-at-gmail.com Mon May 16 10:26:20 2011 4, 30 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GFQJUG007809 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 10:26:20 -0500 4, 30 -- Received: by iwg8 with SMTP id 8so5513673iwg.0 4, 30 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 08:26:18 -0700 (PDT) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 4, 30 -- :content-type; 4, 30 -- bh=Bo+qrr95lDDzXJp1YH0Cym6rXOC6MO/owzftjFNOJrM=; 4, 30 -- b=HHvJBriOh/hUeNId7bn7Pji30YcwjsWO2fGqwmChQqNYJg7jk8CnRiT2dsL+W6kQcA 4, 30 -- Q/w3zLtG+6YIrahTpfEDGcO51BQAvxwQtLJVALPKUVmygiZdAQ+FtMQxB9IYGIcA6ZMg 4, 30 -- 4/pzWq2UpIJu11yBqj2zK/LpLbY98oarPG+Tk= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 30 -- b=fcGnTR4nb8f4d+TymE/6zi5q5y+4MGzrehJ0WkmpAd07Ue57r+r1Xe3sLiM0p0lAHJ 4, 30 -- 4S9jycXgd9DaTnJehRf30vmszXpQRzd9R2fZy1cylZGoSrBLmOvIcyP4JXu36gST7WZp 4, 30 -- 482QObEmTWMX1/gw7O5rDCVDdyRDG08K7p1+k= 4, 30 -- MIME-Version: 1.0 4, 30 -- Received: by 10.231.194.165 with SMTP id dy37mr3331434ibb.30.1305559578473; 4, 30 -- Mon, 16 May 2011 08:26:18 -0700 (PDT) 4, 30 -- Received: by 10.231.145.147 with HTTP; Mon, 16 May 2011 08:26:18 -0700 (PDT) 4, 30 -- Date: Mon, 16 May 2011 11:26:18 -0400 4, 30 -- Message-ID: {BANLkTinOfq9QLkNwFDa=Uu1YiHjkJrTfQQ-at-mail.gmail.com} 4, 30 -- Subject: EDS SiPin Detector 4, 30 -- From: Travis Johnson {travisj25-at-gmail.com} 4, 30 -- To: microscopy-at-microscopy.com 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
I have been following this thread intermittently so I do not know if what I am suggesting has been mentioned. Also, it is a little off topic so please forgive me; I am not trying to high-jack the thread but just to insert a comment. In my experience, if the administration of your school or university is looking for a way to indirectly close down a program, this is one of the strategies they will use. I have seen it happen many times at the university where I have worked since 1979.
Good luck,
Rick A. Harris
______________________________________ X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com] Sent: Monday, May 16, 2011 1:43 AM To: Rick A Harris
Basically what you are saying is that people with more money are happier. This is quite an evidence, isn't it? I don't think people leave a service contract when they don't have to.
Regards,
Stephane
----- Original Message ---- X-from: "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} To: nizets2-at-yahoo.com Sent: Fri, May 13, 2011 3:25:39 PM
Valery, Very well stated. The only thing I might add is that the customer with a service contract is guaranteed certain things, such as preventive maintenance, operation to specification, perhaps even guaranteed maximum down-time. The one-off customer has none of this. The service organization is contractually bound to the contract customer. Period. Yes, the one off cash is nice, but the regular customers are far more valuable in the long run. They often are happier customer, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 13, 2011 8:59 AM To: kenconverse-at-qualityimages.biz
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic. } } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 } 13, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 13, 34 -- Content-Type: text/plain; charset=us-ascii } 13, 34 -- Subject: Microscope service strategies } 13, 34 -- Date: Thu, 12 May 2011 09:47:15 -0700 } 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com } 13, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 13, 34 -- X-Mailer: Apple Mail (2.1084) } 13, 34 -- Content-Transfer-Encoding: 8bit } 13, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 } 23, 46 -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe .ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 } 23, 46 -- Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} } 23, 46 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 } 23, 46 -- Subject: RE: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Topic: [spam]* [Microscopy] Microscope service strategies } 23, 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB } 23, 46 -- Content-Language: en-US } 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB } 23, 46 -- Content-Type: text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit } 23, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date: X-from:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References: In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm 2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO5XVi jSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Fri May 13 08:22:05 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DDM5G5001569 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:22:05 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=2lq4+qobG88tJFkiXmxSLStFAwyj+PriBwHB+7DDebs= c=1 sm=0 a=d6ZFszS330cA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=DahTZl4gAAAA:8 a=Zx37jsudAAAA:8 a=_CgzANL9AAAA:8 a=-gyhParZAAAA:8 a=hCCmtxs2AAAA:8 a=yMhMjlubAAAA:8 a=CjxXgO3LAAAA:8 a=15LFL-canmR1PGpTlQ8A:9 a=YOHmFmhgio4DZwt2t8EA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=U5vkcOzocHIA:10 a=brlFRoIUItEA:10 a=RO2JPw24U6UA:10 a=PmYly_lw2igA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=f96-IYUahzkA:10 a=G91thX30KR8A:10 a=rC2wZJ5BpNYA:10 a=yUsKKQj0Tui2F24u:21 a=NZ21XijlkcsDGYSt:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2310] helo=Ken) 20, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id AC/77-17273-C703DCD4; Fri, 13 May 2011 13:22:05 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {vray-at-partbeamsystech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 20, 28 -- Date: Fri, 13 May 2011 09:21:53 -0400 20, 28 -- Message-ID: {784D2C0EDDA74302903AA04B2F8E61BF-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- Importance: Normal 20, 28 -- Thread-Index: AcwRbZETKQmlIEYgQWGwqB32vBi+0AAAjrwg 20, 28 -- In-Reply-To: {201105131259.p4DCxF4C021152-at-ns.microscopy.com} 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DDM5G5001569 ==============================End of - Headers==============================
==============================Original Headers============================== 35, 50 -- From nizets2-at-yahoo.com Mon May 16 03:39:53 2011 35, 50 -- Received: from nm14.bullet.mail.sp2.yahoo.com (nm14.bullet.mail.sp2.yahoo.com [98.139.91.84]) 35, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4G8dqB9001730 35, 50 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 03:39:52 -0500 35, 50 -- Received: from [98.139.91.67] by nm14.bullet.mail.sp2.yahoo.com with NNFMP; 16 May 2011 08:39:52 -0000 35, 50 -- Received: from [98.139.91.42] by tm7.bullet.mail.sp2.yahoo.com with NNFMP; 16 May 2011 08:39:52 -0000 35, 50 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 16 May 2011 08:39:51 -0000 35, 50 -- X-Yahoo-Newman-Property: ymail-3 35, 50 -- X-Yahoo-Newman-Id: 985366.66804.bm-at-omp1042.mail.sp2.yahoo.com 35, 50 -- Received: (qmail 88670 invoked by uid 60001); 16 May 2011 08:39:51 -0000 35, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305535191; bh=fZxUvAQ9uanwFcclKg1xLte2Y46aBpLDOTew1DueBuA=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=D9OY+i7Yy06WK8ye3kbgOVawYeCG3X4SmrvTh82j8DuTh4ge3VRt3cwHk/pWUD+DnyhEgOGYYQEThtpuGUMdx7xrbGaKTahd73BgLfgG7IUBbMzDw2wP86zn9ITs30GkmPKya3lmH4huCHexfRAtqkFi3PgJEFfzOpxTMhGtFvo= 35, 50 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 35, 50 -- s=s1024; d=yahoo.com; 35, 50 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 35, 50 -- b=yI/vGamFIYuqC8MCqdC+O0r+kzSLAigv8n+TCTofFh7Ga9OoydBMjXXMR/yr2aBAboyKd36vCFqPAGFVvGN/R7tNJHqDYrTsPqlQJZ9brd6kTI3HEhhOlcXH9Plk5UKnip7CWTYxUiOXiQbpb07dEkIAzTg+wwrRIAT5sjm8DYk=; 35, 50 -- Message-ID: {670062.87435.qm-at-web110801.mail.gq1.yahoo.com} 35, 50 -- X-YMail-OSG: Cqu_fvMVM1mop0TJob1Pp_Izp2tPkPfirUrRne90yLdA0VK 35, 50 -- ve3pAEiziidnyPImy3RC3JWto.8Oz9Zy2Hncumgx9l_qC35fMlLmaimIV9qm 35, 50 -- jfnBUR0JCTBo_xwq8eTLVylTHy9MyQl6CyGYNtJCUq57iTIrqd1ziMsMMK7P 35, 50 -- nCTCtGvgTg5aiDySlBlc2AbuhwpnoIspW56ApH_59ZGacohTqNdeEZkwTGl4 35, 50 -- nGzzFkK5Bk.WMvy_51uzPGOTrcSntrfbyYs40OqKZ4pRMlWNIYIKhDqC_V76 35, 50 -- L0WbDCuGvRIWWX2U7n4odTALrl1AWU7fBLj_O732r_FNAE79YcVdHwrgGI4t 35, 50 -- .wzpkvdmKcJdqeTmsnICB01O7TXeajgktQ_qnBS1JUOWyrquPu7PlL3vRaRI 35, 50 -- YYiuS_yy2zZYRw0aG8Skleflb8C0AGNNVYi_eiDl7X2QStZH9TYCT5xoqwNm 35, 50 -- 7.xi8Z9S2CYwngkGYXrqnvDSS1PxJvu1NKQ8amGcMgrs3zfxkOMjnFd9Wq_m 35, 50 -- pWGrwINTsOnwsw1omHWplvWjWfl0CfLqTSOoUPHWzt..d1HzX54K_QYNOj6P 35, 50 -- ZHKWvTqOJB3lRMiZ45R62Z6Zeb1P1E3ItsfC8Kyv0kxHnijWZ1_8JeflEeJ8 35, 50 -- aYuO7uxTdebY89Nr1W6rXObX0WIQtCt3vr6yKgOcRxBR3kfo1MjnAEu2Z6e4 35, 50 -- zg_epvUT3MCZzwiQg1.XcCGC5baYqVl0zr19u1SoPiGIIpOMLjXChvhSTJWq 35, 50 -- v3zoh4Gu.lQGxpVueBS8QrsH_V9E.pIE5vvFWo6quVID3G9Xsn8dHzFxTb8G 35, 50 -- UYfgAS1Q15iZNjrCISUircGbdjX3VxgriWSpuo7Z2XHhBJP8hcEhV9jUEN2x 35, 50 -- _vAGqh2jeL0JLB6ZDS8lyv5BhXSdpj2nTNvkOOLMhqfUll6EFf1Po6i_kVHk 35, 50 -- IYTyj0snuIY.et9HuWRBUz3x9Mv4c38PC.CIO6624VwVKICUcHP9JNVEMrSe 35, 50 -- 8EsUoJ94wyRuKeLBQDgWqLN677IDuPQr_Agg6pZpGzTnKLdAtme2DjG0Vqk2 35, 50 -- Riaa9KKV3SEbB0raZEBmmh_8hGk0cJItRwsOeUWdNDCk7N0QN0Dp_XyfA0jp 35, 50 -- Vau._ALv2dlszc9JDu4z20IMOhvZGyY5A1bAJBqO.T6zIuL3VVNS8Wpo6Aid 35, 50 -- K5BNeA9I.L3tPX8cz2MMrgwCGa5JOdnG95N7ebgzojtXyFEOmosj_mxyROcV 35, 50 -- QWG6TWRgzrWagjf9eCBqXVIlvuEwCXgcerYfpJxCEuyiQORkXdQJOSLxL 35, 50 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Mon, 16 May 2011 01:39:51 PDT 35, 50 -- X-Mailer: YahooMailRC/567 YahooMailWebService/0.8.111.303096 35, 50 -- References: {201105131325.p4DDPdpV007301-at-ns.microscopy.com} 35, 50 -- Date: Mon, 16 May 2011 01:39:51 -0700 (PDT) 35, 50 -- From: Stephane Nizet {nizets2-at-yahoo.com} 35, 50 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 35, 50 -- To: microscopy-at-microscopy.com 35, 50 -- In-Reply-To: {201105131325.p4DDPdpV007301-at-ns.microscopy.com} 35, 50 -- MIME-Version: 1.0 35, 50 -- Content-Type: text/plain; charset=iso-8859-1 35, 50 -- Content-Transfer-Encoding: 8bit 35, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4G8dqB9001730 ==============================End of - Headers==============================
==============================Original Headers============================== 47, 32 -- From raharris-at-ucdavis.edu Mon May 16 12:28:26 2011 47, 32 -- Received: from smtp3.ucdavis.edu (smtp3.ucdavis.edu [128.120.32.129]) 47, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GHSP6I025493 47, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 12:28:25 -0500 47, 32 -- Received: from exhub.ex.ad3.ucdavis.edu ([169.237.229.95]) 47, 32 -- by smtp3.ucdavis.edu (8.13.1/8.13.1/it-rhel5-mimedefang-smtp-1.2) with ESMTP id p4GHSGr4046480 47, 32 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 47, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 10:28:23 -0700 47, 32 -- Received: from EXMBX5.ex.ad3.ucdavis.edu ([169.254.2.238]) by 47, 32 -- exhub4.ex.ad3.ucdavis.edu ([169.237.229.95]) with mapi id 14.01.0289.001; 47, 32 -- Mon, 16 May 2011 10:28:20 -0700 47, 32 -- From: Rick A Harris {raharris-at-ucdavis.edu} 47, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 47, 32 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 47, 32 -- Thread-Topic: [Microscopy] [spam]* Microscope service strategies 47, 32 -- Thread-Index: AQHME6ViA4P2u48c2Ua8PXk0W3rmSJSPs23s 47, 32 -- Date: Mon, 16 May 2011 17:28:19 +0000 47, 32 -- Message-ID: {F039D1AA92BC114EBAFC2A5E8259B15A22079C08-at-exmbx5.ex.ad3.ucdavis.edu} 47, 32 -- References: {201105160843.p4G8hhl4006092-at-ns.microscopy.com} 47, 32 -- In-Reply-To: {201105160843.p4G8hhl4006092-at-ns.microscopy.com} 47, 32 -- Accept-Language: en-US 47, 32 -- Content-Language: en-US 47, 32 -- X-MS-Has-Attach: 47, 32 -- X-MS-TNEF-Correlator: 47, 32 -- x-originating-ip: [169.237.121.164] 47, 32 -- Content-Type: text/plain; charset="us-ascii" 47, 32 -- MIME-Version: 1.0 47, 32 -- X-Virus-Scanned: clamav-milter 0.97 at av3 47, 32 -- X-Virus-Status: Clean 47, 32 -- X-Scanned-By: MIMEDefang 2.67 on 128.120.32.8 47, 32 -- Content-Transfer-Encoding: 8bit 47, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4GHSP6I025493 ==============================End of - Headers==============================
Dear Justin, I have no experience with preparing SEM specimens, but be sure your student gets a yogurt advertised as containing live culture. Otherwise, there would possibly be no bacteria to see. It will be essential to dilute out the remains of the milk solids, etc., to get a good look at the bacteria, and it might be a good idea to take a bit of yogurt and prepare a lactose solution to grow the bacteria in. Just put the yogurt into the solution, place in a warm room or oven at ~37 C, and wait overnight to harvest the bacteria. Lactose solution can be centrifuged at relatively low speed to concentrate the bacteria for the prep. Yours, Bill
X-from: kraftpiano-at-gmail.com To: William.F.Tivol-at-aero.org
Does anyone have a protocol for going from straight-from-the-store yogurt to prepared SEM specimen? I have a student interested in seeing what the bacteria looks like. They are also trying to find out just how much of yogurt is, in fact, bacteria. Not being a biologist, I'm at a bit of a loss...
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GI96jO025853 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 16 May 2011 13:09:06 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p4GI96Wo025851; Mon, 16 May 2011 13:09:06 -0500
Dear Mike, A high-angle detector is for STEM mode. It is usually called a high-angle annular dark-field or HAADF detector. It should look like a large washer, more-or-less, with electrical connections attached. It detects electrons that have been scattered through angles greater than ~50 mrad, according to Williams and Carter, page 359. This detector is located below the specimen, but I don't know where it would be, exactly, in the 1200EX, assuming that the 1200 EX has a STEM mode of operation. Yours, Bill
X-from: microscopylistserver-noreply-at-microscopy.com To: William.F.Tivol-at-aero.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjwanzek-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mjwanzek-at-gmail.com Name: Mike
Organization: Washington State University
Title-Subject: [Filtered] JEM 1200EX
Message: I purchased a JEM 1200EX TEM microscope from a university surplus auction. I don't know much about electron microscopes, and I listed it on Ebay for $5000 (just go to ebay and search "jem 1200ex").
Anyways, I got a quesion asking if it has a "high angle detector". I don't know what this is, and I couldn't find out from some googling, so does anyone know what this is, where it is located, and what it looks like?
Also, who would you expect would be interested in an auction like this? Do you think universities or companies, would be interested, or mainly people who just want an electron microscope that they can set up in their house? Thanks.
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GIumE1010129 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 16 May 2011 13:56:48 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p4GIum8M010127; Mon, 16 May 2011 13:56:48 -0500
Justin,
I once ran up Colby cheese for TEM and the bacteria were beautiful. If you can interest the student into switching I'd suggest cutting thin strips of cheese then break them and run the sample up for SEM or after CPD snap the strip into two. I'd think the bacteria should be visible along the fractured surface.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Does anyone have a protocol for going from straight-from-the-store yogurt to prepared SEM specimen? I have a student interested in seeing what the bacteria looks like. They are also trying to find out just how much of yogurt is, in fact, bacteria. Not being a biologist, I'm at a bit of a loss...
--Justin A. Kraft
==============================Original Headers============================== 19, 28 -- From connellyps-at-nhlbi.nih.gov Mon May 16 13:56:47 2011 19, 28 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GIukcf010122 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 13:56:47 -0500 19, 28 -- X-IronPortListener: Outbound_SMTP 19, 28 -- X-IronPort-Anti-Spam-Filtered: true 19, 28 -- X-IronPort-Anti-Spam-Result: Am4GAGdy0U2cKEcU/2dsb2JhbACbDIsLd6Z2g3KbSAKDLIJrBJARhEyDf4ZG 19, 28 -- Received: from unknown (HELO NIHHT01.nih.gov) ([156.40.71.20]) 19, 28 -- by nihxway3out.hub.nih.gov with ESMTP; 16 May 2011 14:56:45 -0400 19, 28 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT01.nih.gov 19, 28 -- ([156.40.71.20]) with mapi; Mon, 16 May 2011 14:56:38 -0400 19, 28 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 19, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 28 -- Date: Mon, 16 May 2011 14:56:37 -0400 19, 28 -- Subject: Re: [Microscopy] Re: SEM preparation of yogurt bacteria? 19, 28 -- Thread-Topic: [Microscopy] Re: SEM preparation of yogurt bacteria? 19, 28 -- Thread-Index: AcwT8VKY3ixSbfSqSye9dIhyNlC6zwACaj1c 19, 28 -- Message-ID: {C9F6EBA5.809D%connellyps-at-nhlbi.nih.gov} 19, 28 -- In-Reply-To: {201105161747.p4GHlPT8015891-at-ns.microscopy.com} 19, 28 -- Accept-Language: en-US 19, 28 -- Content-Language: en 19, 28 -- X-MS-Has-Attach: 19, 28 -- X-MS-TNEF-Correlator: 19, 28 -- acceptlanguage: en-US 19, 28 -- Content-Type: text/plain; charset="iso-8859-1" 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4GIukcf010122 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GJGt8n026506 for {MicroscopyListserverArchive2-at-microscopy.com} ; Mon, 16 May 2011 14:16:55 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p4GJGtXq026504; Mon, 16 May 2011 14:16:55 -0500
All, There is a new option that is floating around the University of Texas system. An independent company has signed a deal with UT system to save everyone 25% on their service contracts (not just EMs - a whole range of equipment, as long as it is a parts and labor contract). They get you to move to an "on demand" arrangement with your existing service provider. They pay all the bills and take the chance that you will spend less than a full price contract. Of course there is the issue with how service providers prioritize contract versus on-demand customers. This company will freeze the cost for 4 years and as part of the arrangement with UT system (with qualifying contracts and equipment) will not decline coverage.
I am tempted but don't like the idea of having less priority when I make a service call.
Regards
Chris
Christopher J Gilpin Ph.D. Assistant Professor Director, Molecular and Cellular Imaging Facility K1.A04 UT Southwestern Medical Center at Dallas 5323 Harry Hines Boulevard Dallas, TX 75390-9039 Phone +1 214 648 2827
-----Original Message----- X-from: raharris-at-ucdavis.edu [mailto:raharris-at-ucdavis.edu] Sent: Monday, May 16, 2011 12:38 PM To: Christopher Gilpin
I have been following this thread intermittently so I do not know if what I am suggesting has been mentioned. Also, it is a little off topic so please forgive me; I am not trying to high-jack the thread but just to insert a comment. In my experience, if the administration of your school or university is looking for a way to indirectly close down a program, this is one of the strategies they will use. I have seen it happen many times at the university where I have worked since 1979.
Good luck,
Rick A. Harris
______________________________________ X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com] Sent: Monday, May 16, 2011 1:43 AM To: Rick A Harris
Basically what you are saying is that people with more money are happier. This is quite an evidence, isn't it? I don't think people leave a service contract when they don't have to.
Regards,
Stephane
----- Original Message ---- X-from: "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} To: nizets2-at-yahoo.com Sent: Fri, May 13, 2011 3:25:39 PM
Valery, Very well stated. The only thing I might add is that the customer with a service contract is guaranteed certain things, such as preventive maintenance, operation to specification, perhaps even guaranteed maximum down-time. The one-off customer has none of this. The service organization is contractually bound to the contract customer. Period. Yes, the one off cash is nice, but the regular customers are far more valuable in the long run. They often are happier customer, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 13, 2011 8:59 AM To: kenconverse-at-qualityimages.biz
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so } tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to } their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic.
} } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, 34 } -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu } (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us } (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 34 -- } Content-Type: text/plain; charset=us-ascii 13, 34 -- Subject: } Microscope service strategies 13, 34 -- Date: Thu, 12 May 2011 } 09:47:15 -0700 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- Mime-Version: 1.0 } (Apple Message framework v1084) 13, 34 -- X-Mailer: Apple Mail } (2.1084) 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 23, 46 } -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 23, 46 -- } Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id } C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe .ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk } designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain } [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown } [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk } (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 23, 46 -- } Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with } mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} 23, 46 -- To: } "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 23, 46 -- Subject: RE: } [spam]* [Microscopy] Microscope service strategies 23, 46 -- } Thread-Topic: [spam]* [Microscopy] Microscope service strategies 23, } 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB 23, 46 -- Content-Language: } en-US 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB 23, 46 -- Content-Type: } text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit 23, 46 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date: X-from:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:Reference s: In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm 2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO 2+5XVi jSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Fri May 13 08:22:05 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DDM5G5001569 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:22:05 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=2lq4+qobG88tJFkiXmxSLStFAwyj+PriBwHB+7DDebs= c=1 sm=0 a=d6ZFszS330cA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=DahTZl4gAAAA:8 a=Zx37jsudAAAA:8 a=_CgzANL9AAAA:8 a=-gyhParZAAAA:8 a=hCCmtxs2AAAA:8 a=yMhMjlubAAAA:8 a=CjxXgO3LAAAA:8 a=15LFL-canmR1PGpTlQ8A:9 a=YOHmFmhgio4DZwt2t8EA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=U5vkcOzocHIA:10 a=brlFRoIUItEA:10 a=RO2JPw24U6UA:10 a=PmYly_lw2igA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=f96-IYUahzkA:10 a=G91thX30KR8A:10 a=rC2wZJ5BpNYA:10 a=yUsKKQj0Tui2F24u:21 a=NZ21XijlkcsDGYSt:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2310] helo=Ken) 20, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id AC/77-17273-C703DCD4; Fri, 13 May 2011 13:22:05 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {vray-at-partbeamsystech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 20, 28 -- Date: Fri, 13 May 2011 09:21:53 -0400 20, 28 -- Message-ID: {784D2C0EDDA74302903AA04B2F8E61BF-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- Importance: Normal 20, 28 -- Thread-Index: AcwRbZETKQmlIEYgQWGwqB32vBi+0AAAjrwg 20, 28 -- In-Reply-To: {201105131259.p4DCxF4C021152-at-ns.microscopy.com} 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DDM5G5001569 ==============================End of - Headers==============================
While I think Bill is right in answering your question, there is another possibility (somewhat remote) about the "high-angle" detector. Some of the older JEOL scopes also had a "high-angle" EDX detector with ~68 deg. elevation angle. Our old 200CX had a detector like this and I think that the "EX" series scopes had the large-bore upper pole-piece and could accommodate this EDX detector. At the time of these scopes, HAADF was not as common a technique as now.
With that minor caveat, I agree with Bill that your potential buyer is most likely interested in HAADF when he says "high-angle detector". In your ebay photo, the cylinder on the left just above the viewing chamber looks like a CCD type camera. This is where I would expect an HAADF detector to be mounted.
Based on the age and type of scope, I would be surprised if your scope had an HAADF detector.
Cheers, Henk
At 5/16/2011 2:10 PM, William.F.Tivol-at-aero.org wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Mike, } A high-angle detector is for STEM mode. It is usually called a } high-angle annular dark-field or HAADF detector. It should look like a } large washer, more-or-less, with electrical connections attached. It } detects electrons that have been scattered through angles greater than ~50 } mrad, according to Williams and Carter, page 359. This detector is } located below the specimen, but I don't know where it would be, exactly, } in the 1200EX, assuming that the 1200 EX has a STEM mode of operation. } Yours, } Bill } } } } X-from: microscopylistserver-noreply-at-microscopy.com } To: William.F.Tivol-at-aero.org } Date: 05/15/2011 02:02 PM } Subject: [Microscopy] viaWWW:JEM 1200EX } } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both mjwanzek-at-gmail.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mjwanzek-at-gmail.com Name: Mike } } Organization: Washington State University } } Title-Subject: [Filtered] JEM 1200EX } } Message: I purchased a JEM 1200EX TEM microscope from a university } surplus auction. I don't know much about electron microscopes, and I } listed it on Ebay for $5000 (just go to ebay and search "jem 1200ex"). } } Anyways, I got a quesion asking if it has a "high angle detector". I } don't know what this is, and I couldn't find out from some googling, so } does anyone know what this is, where it is located, and what it looks } like? } } Also, who would you expect would be interested in an auction like this? } Do you think universities or companies, would be interested, or mainly } people who just want an electron microscope that they can set up in } their house? Thanks. } } } ==============================Original Headers============================== } 18, 46 -- From prvs=110e1b1e5=William.F.Tivol-at-aero.org Mon May 16 13:09:06 2011 } 18, 46 -- Received: from email2-west.aero.org (email2-west.aero.org [130.221.16.27]) } 18, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GI95xa025848 } 18, 46 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 13:09:06 -0500 } 18, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 18, 46 -- d=aero.org; i=William.F.Tivol-at-aero.org; q=dns/txt; } 18, 46 -- s=mailhub; t=1305569346; x=1337105346; } 18, 46 -- h=in-reply-to:references:to:mime-version:subject: } 18, 46 -- message-id:date:from; } 18, 46 -- z=In-Reply-To:=20 {201105152102.p4FL2W4j023999-at-ns.microscop } 18, 46 -- y.com} |References:=20 {201105152102.p4FL2W4j023999-at-ns.micr } 18, 46 -- oscopy.com} |To:=20microscopy-at-microscopy.com,=0D=0A=09mjwa } 18, 46 -- nzek-at-gmail.com|MIME-Version:=201.0|Subject:=20Re:=20[Micr } 18, 46 -- oscopy]=20viaWWW:JEM=201200EX|Message-ID:=20 {OF1BD0D965.4 } 18, 46 -- 969FB34-ON88257892.006266E6-88257892.0063BB75-at-notes.aero. } 18, 46 -- org} |Date:=20Mon,=2016=20May=202011=2011:08:49=20-0700 } 18, 46 -- |From:=20William=20F=20Tivol=20 {William.F.Tivol-at-aero.org} ; } 18, 46 -- bh=mAAI2FZwMX2MWGCOfTrjlDKEI/xf7Ozt3Zn0L59Ldfw=; } 18, 46 -- b=E0PqrOPpXUXy0mvbOPmjgkFEpZCvwZB7kWxc/Ym6tk71NowFEoI+hwTz } 18, 46 -- 8M7aEMALkoo8jNoBk+XL3sm+NrUV3t37N2WoRNlZWA/JF2uXpic1tHAmH } 18, 46 -- 3PWMEiIGNItM0l0H/CCeMOMJG9nTR5WK2iBI1UTNW6YeFSu3f10Ka480u } 18, 46 -- E=; } 18, 46 -- From: William F Tivol {William.F.Tivol-at-aero.org} } 18, 46 -- x-SBRS: 2.9 } 18, 46 -- x-SenderGroup: Internal_Relay_hosts } 18, 46 -- X-IronPort-Anti-Spam-Filtered: true } 18, 46 -- X-IronPort-Anti-Spam-Result: Ak4IANRn0U2C3VwSgWdsb2JhbACfE4cEFAEBFiYlxmSGGQSGUIEeiCOIS4ZK } 18, 46 -- X-IronPort-AV: E=McAfee;i="5400,1158,6348"; a="45091464" } 18, 46 -- X-IronPort-AV: E=Sophos;i="4.65,220,1304319600"; } 18, 46 -- d="scan'208";a="45091464" } 18, 46 -- Received: from agosmtpout.aero.org ([130.221.92.18]) } 18, 46 -- by mailhub2.aero.org with ESMTP; 16 May 2011 11:09:05 -0700 } 18, 46 -- In-Reply-To: {201105152102.p4FL2W4j023999-at-ns.microscopy.com} } 18, 46 -- References: {201105152102.p4FL2W4j023999-at-ns.microscopy.com} } 18, 46 -- To: microscopy-at-microscopy.com, mjwanzek-at-gmail.com } 18, 46 -- MIME-Version: 1.0 } 18, 46 -- Subject: Re: [Microscopy] viaWWW:JEM 1200EX } 18, 46 -- X-KeepSent: 1BD0D965:4969FB34-88257892:006266E6; } 18, 46 -- type=4; name=$KeepSent } 18, 46 -- X-Mailer: Lotus Notes Release 8.5.1 September 28, 2009 } 18, 46 -- Message-ID: {OF1BD0D965.4969FB34-ON88257892.006266E6-88257892.0063BB75-at-notes.aero.org} } 18, 46 -- Date: Mon, 16 May 2011 11:08:49 -0700 } 18, 46 -- X-MIMETrack: Serialize by Router on agosmtpout/AeroNet/Aerospace/US(Release 8.5.2 HF250|November } 18, 46 -- 18, 2010) at 05/16/2011 11:09:05 AM, } 18, 46 -- Serialize complete at 05/16/2011 11:09:05 AM } 18, 46 -- Content-Type: text/plain; charset="US-ASCII" } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 9, 26 -- From colijn.1-at-osu.edu Mon May 16 14:55:17 2011 9, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GJtH4P010782 9, 26 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 14:55:17 -0500 9, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 26 -- id {01O1CQIQHWE89ETDDZ-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 9, 26 -- Mon, 16 May 2011 15:55:16 -0400 (EDT) 9, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 9, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 26 -- with ESMTPA id {01O1CQIAOEWQ9EZBYF-at-ecr6.ohio-state.edu} ; Mon, 9, 26 -- 16 May 2011 15:54:58 -0400 (EDT) 9, 26 -- Date: Mon, 16 May 2011 15:54:53 -0400 9, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 26 -- Subject: Re: [Microscopy] Re: viaWWW:JEM 1200EX 9, 26 -- In-reply-to: {201105161810.p4GIANwa027746-at-ns.microscopy.com} 9, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 26 -- To: William.F.Tivol-at-aero.org, mjwanzek-at-gmail.com, microscopy-at-microscopy.com 9, 26 -- Message-id: {4DD1810D.8050700-at-osu.edu} 9, 26 -- MIME-version: 1.0 9, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 26 -- Content-transfer-encoding: 7bit 9, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) 9, 26 -- Gecko/20110414 Thunderbird/3.1.10 9, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 26 -- References: {201105161810.p4GIANwa027746-at-ns.microscopy.com} ==============================End of - Headers==============================
Don't do it. I dealt with a system like this back in my previous position. Worked fine as long as we only did routine maintenance and a maybe a small changing-out-a-part repair. But the first time we did something unusual to them but perfectly normal for EM - tracing a stray EM field - the company demanded we pay the full bill. Because they don't pay for room tests. Didn't matter that the EMF test is routine for diagnosing imaging problems, didn't matter that the test was a minor part of the bill, didn't matter that ... etc. etc. . Also didn't matter that I specifically asked them a series of "what if" questions about what they covered - like the above - and their answer was always "you're covered". We got back on an instrument vendor service contract and were much happier.
Now what I do is keep track of time-and-materials charges for any repairs or maintenance and anytime some admin type thinks of dropping the service contract, I show them the comparison: service contract vs. time-and-materials (T&M). The contract has always been cheaper for any year other than ones in which the instruments never needed more than routine PMs. In more than one case, as someone mentioned earlier, a single repair visit if charged as T&M would have cost } 2x the annual price of a full-option service contract.
Mind, CMU generally is a believer in service contracts, unlike some places I've been, so that helps, but it still is very useful to have the contract cost vs. T&M cost comparison handy.
Phil
} All, } There is a new option that is floating around the University of Texas } system. An independent company has signed a deal with UT system to save } everyone 25% on their service contracts (not just EMs - a whole range of } equipment, as long as it is a parts and labor contract). They get you to } move to an "on demand" arrangement with your existing service provider. They } pay all the bills and take the chance that you will spend less than a full } price contract. Of course there is the issue with how service providers } prioritize contract versus on-demand customers. This company will freeze the } cost for 4 years and as part of the arrangement with UT system (with } qualifying contracts and equipment) will not decline coverage. } } I am tempted but don't like the idea of having less priority when I make a } service call. } } Regards } } Chris } } } Christopher J Gilpin Ph.D. } Assistant Professor } Director, Molecular and Cellular Imaging Facility } K1.A04 } UT Southwestern Medical Center at Dallas } 5323 Harry Hines Boulevard } Dallas, TX 75390-9039 } Phone +1 214 648 2827
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 30 -- From oshel1pe-at-cmich.edu Mon May 16 14:57:01 2011 7, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GJv1rE014275 7, 30 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 14:57:01 -0500 7, 30 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 7, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p4GJuuvW029735; 7, 30 -- Mon, 16 May 2011 15:57:00 -0400 7, 30 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 7, 30 -- Mon, 16 May 2011 15:56:39 -0400 7, 30 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 7, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Mon, 16 May 7, 30 -- 2011 15:56:39 -0400 7, 30 -- MIME-Version: 1.0 7, 30 -- Message-ID: {a06240806c9f72e10138c-at-[141.209.160.249]} 7, 30 -- In-Reply-To: {201105161920.p4GJKEhE031694-at-ns.microscopy.com} 7, 30 -- References: {201105161920.p4GJKEhE031694-at-ns.microscopy.com} 7, 30 -- Date: Mon, 16 May 2011 15:56:37 -0400 7, 30 -- To: {Christopher.Gilpin-at-utsouthwestern.edu} 7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 30 -- Subject: Re: [Microscopy] FW: RE: [spam]* Microscope service strategies 7, 30 -- CC: {Microscopy-at-microscopy.com} 7, 30 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 30 -- X-Originating-IP: [141.209.160.249] 7, 30 -- X-OriginalArrivalTime: 16 May 2011 19:56:39.0357 (UTC) FILETIME=[5EB96ED0:01CC1403] 7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 30 -- X-Spam-Score: -3.00 () [Hold at 6.00] L_EXCH_MF,L_USD,L_USDOC,RDNS_NONE,Bayes(0.0001:-0.5) 7, 30 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 30 -- X-CanItPRO-Stream: default 7, 30 -- X-Canit-Stats-ID: 02EIjV0OU - 6b4c773f8668 - 20110516 7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Our management was really enthusiastic about migrating to one of these insurance plans because of the promised savings. We have been under one for most instruments for five years. Despite initial skepticism on the part of the technical staff, it has worked well for some of the auxiliary prep equipment in the microscopy lab (such as ultramicrotomes) and for most of our division's analytical instrumentation. There have been a few significant issues with our electron microscopes that have caused us to migrate them back to vendor service. We have done this as we could demonstrate that it was cost effective for our instruments. Here are some issues to consider:
1. Read the fine print on the contract carefully. For example, in ours there was a provision excluding high voltage transformers that applied to high voltage tanks. Think about the financial exposure you will have if you have an arc that causes a problem in the tank that the vendor recommends replacing it. We had a close call. Happily, ours turned out to be the emission chamber, that was covered. The prospect of paying for a HV tank really frightened my division director.
2. Will the vendor take the insurance company PO directly? I am pretty sure at the current time none of the "big 3" EM companies will. Call the service manager for your scopes and ask. Think about all the extra costs and time involved in generating and getting approval for purchase orders for parts and labor for every service call and the paperwork for submitting for reimbursement and tracking that everything goes through.
3. Most vendors will not ship a part if they don't have a PO with sufficient value to cover it. Imagine that it is 4 pm and the engineer thinks they need 3 or 4 boards and a component or two to work on a particular problem the next day. In all likelihood they may only need 1 or 2 of them and will send the rest back, but can't tell until they get into the repair. Given the scenario in #2 above, what do you think the probability that the parts you need will make the FedEx cut off. If they don't, you now have an engineer who needs to bill time without the parts. Do you "eat" the labor costs so the engineer is there the next day when the parts come or let them go, not knowing when they can come back? This created MANY Mylanta moments for me.
4. Contract customers are usually higher in the queue than time-and-materials customers. This is business. You reward your loyal customers. What is the cost of waiting for the engineer?
Here is my recommendation: take the last 4 or 5 service calls on your scopes and get the prices for time and materials for your scopes. Work out the senarios under the insurance schemes looking at items 1-4 above. Crunch the numbers for your management.
At the end of the day, it all comes down to your management and a line from a Clint Eastwood movie: "Do you feel lucky?" I have seen the time and material costs for a single difficult service call reach 3-4X the contract price. Ask your boss how their boss would respond to one of these unplanned expenses. One of the key values of service contracts that MUST be considered is predictability of costs.
best regards, John Minter
Christopher.Gilpin-at-utsouthwestern.edu wrote: } All, } There is a new option that is floating around the University of Texas } system. An independent company has signed a deal with UT system to save } everyone 25% on their service contracts (not just EMs - a whole range of } equipment, as long as it is a parts and labor contract). They get you to } move to an "on demand" arrangement with your existing service provider. They } pay all the bills and take the chance that you will spend less than a full } price contract. Of course there is the issue with how service providers } prioritize contract versus on-demand customers. This company will freeze the } cost for 4 years and as part of the arrangement with UT system (with } qualifying contracts and equipment) will not decline coverage. } } I am tempted but don't like the idea of having less priority when I make a } service call.
==============================Original Headers============================== 13, 23 -- From jrminter-at-rochester.rr.com Mon May 16 16:15:04 2011 13, 23 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.124]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GLF4nL011529 13, 23 -- for {Microscopy-at-Microscopy.Com} ; Mon, 16 May 2011 16:15:04 -0500 13, 23 -- X-Authority-Analysis: v=1.1 cv=NmbQexcdgr4rtO3OwYGrP5Q3rTMpacrTPhuaXkv4uP8= c=1 sm=0 a=wt9oAqeLfrcA:10 a=kj9zAlcOel0A:10 a=QOXvuJxCzNGPTHlfmoyVfA==:17 a=Q4qwp2JwhZvQ9RJ1je4A:9 a=WJsBJlTLjGAXhT29jMIA:7 a=CjuIK1q_8ugA:10 a=QOXvuJxCzNGPTHlfmoyVfA==:117 13, 23 -- X-Cloudmark-Score: 0 13, 23 -- X-Originating-IP: 74.65.12.77 13, 23 -- Received: from [74.65.12.77] ([74.65.12.77:42435] helo=[10.0.1.4]) 13, 23 -- by hrndva-oedge01.mail.rr.com (envelope-from {jrminter-at-rochester.rr.com} ) 13, 23 -- (ecelerity 2.2.3.46 r()) with ESMTP 13, 23 -- id 3C/19-08391-8D391DD4; Mon, 16 May 2011 21:15:04 +0000 13, 23 -- Content-Type: text/plain; charset=us-ascii 13, 23 -- Mime-Version: 1.0 (Apple Message framework v1084) 13, 23 -- Subject: Re: [Microscopy] Microscope service strategies 13, 23 -- From: John Minter {jrminter-at-rochester.rr.com} 13, 23 -- In-Reply-To: {201105161917.p4GJH0nR026610-at-ns.microscopy.com} 13, 23 -- Date: Mon, 16 May 2011 17:15:04 -0400 13, 23 -- Message-Id: {B54BB23C-020F-4CE9-9AB5-C61DD8437F37-at-rochester.rr.com} 13, 23 -- References: {201105161917.p4GJH0nR026610-at-ns.microscopy.com} 13, 23 -- To: Microscopy-at-Microscopy.Com 13, 23 -- X-Mailer: Apple Mail (2.1084) 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4GLF4nL011529 ==============================End of - Headers==============================
I know the topic of insurance in place of manufacturer's service contract has come up before, and I have seen that discussion. I am interested to know if anyone has specific experience with the REMI Group insuring electron microscopes (or other high-tech equipment). Feel free to respond off-list (jan.factor-at-purchase.edu). If you would prefer to talk instead of write, you can call me (below) or send your phone number and I will gladly call you. Many thanks in advance. --Best, Jan Factor
---------------------------------------------------------- Jan Robert Factor, Ph.D. Professor of Biology ---------------------------------------------------------- Purchase College State University of New York 735 Anderson Hill Rd. Purchase, NY 10577 USA ---------------------------------------------------------- Office Tel: 914-251-6659 Office: 2016NS Email: jan.factor-at-purchase.edu ----------------------------------------------------------
==============================Original Headers============================== 4, 25 -- From Jan.Factor-at-purchase.edu Mon May 16 19:00:20 2011 4, 25 -- Received: from purchase.edu (vsexchcas01-rcn.purchase.edu [199.79.170.79]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4H00JM4029654 4, 25 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 19:00:20 -0500 4, 25 -- Received: from svexchmb01.purchase.edu ([10.0.248.71]) by 4, 25 -- VSEXCHCAS01.purchase.edu ([fe80::c841:1dfd:d7bd:78be%12]) with mapi; Mon, 16 4, 25 -- May 2011 20:00:14 -0400 4, 25 -- From: "Factor, Jan" {Jan.Factor-at-purchase.edu} 4, 25 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 4, 25 -- Date: Mon, 16 May 2011 20:00:13 -0400 4, 25 -- Subject: EM insurance experience 4, 25 -- Thread-Topic: EM insurance experience 4, 25 -- Thread-Index: AcwUEP/wrAL0iUvPRjiEOP3gTbYyGQAExhhQ 4, 25 -- Message-ID: {A395C4E6EB90404ABBC720ACFA8CF13006B67BAD61-at-svexchmb01} 4, 25 -- References: {A395C4E6EB90404ABBC720ACFA8CF13006B67BAD5C-at-svexchmb01} 4, 25 -- In-Reply-To: {A395C4E6EB90404ABBC720ACFA8CF13006B67BAD5C-at-svexchmb01} 4, 25 -- Accept-Language: en-US 4, 25 -- Content-Language: en-US 4, 25 -- X-MS-Has-Attach: 4, 25 -- X-MS-TNEF-Correlator: 4, 25 -- acceptlanguage: en-US 4, 25 -- Content-Type: text/plain; charset="us-ascii" 4, 25 -- MIME-Version: 1.0 4, 25 -- Content-Transfer-Encoding: 8bit 4, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4H00JM4029654 ==============================End of - Headers==============================
Just to get some numbers here, how much is reasonable for a service contract? 10% of original cost of the instrument? 15% of cost?
We have a service contract on one instrument (confocal) that was (10 years ago) about 8% of original cost, now is about 10%. This is essentially a full extended warranty, and includes an annual service visit, unlimited emergency callouts and all replacement parts, even lasers are included. It's been great. I don't think they have this type of contract for new instruments, thoughŠ
However, for a new instrument (ESEM) from a different supplier, we were quoted about 15% of the original cost for a contract with 2 annual maintenance visits plus unlimited emergency callouts, but no coverage of replacement parts. For 2 maintenance visits and 2 emergency callouts but no parts the cost went down to about 8% of initial cost. The reason given for the cost was that we had many breakdowns in the 12 month warranty period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, computer fan blew up, etc, etc), and they had to factor this in. Considering the presumed reliability of SEMs compared to confocals (in my experience before this new instrument), and that no parts were covered, it seemed quite expensive.
Is the latter about what you'd expect to pay for a service contract?
Thanks, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
==============================Original Headers============================== 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 2011 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4H1wX9q015257 13, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 -0500 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 13, 32 -- s=email; t=1305597514; x=1337133514; 13, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 13, 32 -- content-transfer-encoding; 13, 32 -- bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; 13, 32 -- b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv 13, 32 -- VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb 13, 32 -- +BsypGn+/TtdMUH; 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; 13, 32 -- d="scan'208";a="20957752" 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 13, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May 2011 11:58:32 +1000 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 13, 32 -- (TLS) id 8.3.159.2; Tue, 17 May 2011 11:58:32 +1000 13, 32 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 13, 32 -- Date: Tue, 17 May 2011 11:58:29 +1000 13, 32 -- Subject: Re: Microscope service strategies - numbers 13, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} 13, 32 -- To: {Microscopy-at-microscopy.com} 13, 32 -- Message-ID: {C9F81355.10B35%rosemary.white-at-csiro.au} 13, 32 -- Thread-Topic: Microscope service strategies - numbers 13, 32 -- In-Reply-To: {201105162121.p4GLLsvq019450-at-ns.microscopy.com} 13, 32 -- MIME-Version: 1.0 13, 32 -- Content-Type: text/plain; charset="ISO-8859-1" 13, 32 -- Content-Transfer-Encoding: 8bit 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4H1wX9q015257 ==============================End of - Headers==============================
I find it funny that you have to pay because you had so many problems during the first 12 monthes. This means that in the end you paid because they bought an unreliable instrument! I wonder if you could not get an extension of the warranty period for such a dramatic unreliability!
Regards,
Stephane
Â
----- Original Message ---- X-from: "rosemary.white-at-csiro.au" {rosemary.white-at-csiro.au} To: nizets2-at-yahoo.com Sent: Tue, May 17, 2011 4:02:17 AM
Dear all,
Just to get some numbers here, how much is reasonable for a service contract? 10% of original cost of the instrument? 15% of cost?
We have a service contract on one instrument (confocal) that was (10 years ago) about 8% of original cost, now is about 10%. This is essentially a full extended warranty, and includes an annual service visit, unlimited emergency callouts and all replacement parts, even lasers are included. It's been great. I don't think they have this type of contract for new instruments, thoughÅ
However, for a new instrument (ESEM) from a different supplier, we were quoted about 15% of the original cost for a contract with 2 annual maintenance visits plus unlimited emergency callouts, but no coverage of replacement parts. For 2 maintenance visits and 2 emergency callouts but no parts the cost went down to about 8% of initial cost. The reason given for the cost was that we had many breakdowns in the 12 month warranty period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, computer fan blew up, etc, etc), and they had to factor this in. Considering the presumed reliability of SEMs compared to confocals (in my experience before this new instrument), and that no parts were covered, it seemed quite expensive.
Is the latter about what you'd expect to pay for a service contract?
Thanks, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
==============================Original Headers============================== 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 2011 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) 13, 32 -- Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4H1wX9q015257 13, 32 -- Â Â Â for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 -0500 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 13, 32 --Â d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 13, 32 --Â s=email; t=1305597514; x=1337133514; 13, 32 --Â h=date:subject:from:to:message-id:in-reply-to:mime-version: 13, 32 --Â Â content-transfer-encoding; 13, 32 --Â bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; 13, 32 --Â b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv 13, 32 --Â Â VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb 13, 32 --Â Â +BsypGn+/TtdMUH; 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; 13, 32 --Â Â d="scan'208";a="20957752" 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 13, 32 --Â by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May 2011 11:58:32 +1000 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by 13, 32 --Â EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 13, 32 --Â (TLS) id 8.3.159.2; Tue, 17 May 2011 11:58:32 +1000 13, 32 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 13, 32 -- Date: Tue, 17 May 2011 11:58:29 +1000 13, 32 -- Subject: Re: Microscope service strategies - numbers 13, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} 13, 32 -- To: {Microscopy-at-microscopy.com} 13, 32 -- Message-ID: {C9F81355.10B35%rosemary.white-at-csiro.au} 13, 32 -- Thread-Topic: Microscope service strategies - numbers 13, 32 -- In-Reply-To: {201105162121.p4GLLsvq019450-at-ns.microscopy.com} 13, 32 -- MIME-Version: 1.0 13, 32 -- Content-Type: text/plain; charset="ISO-8859-1" 13, 32 -- Content-Transfer-Encoding: 8bit 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4H1wX9q015257 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 43 -- From nizets2-at-yahoo.com Tue May 17 02:39:54 2011 26, 43 -- Received: from nm20-vm0.bullet.mail.sp2.yahoo.com (nm20-vm0.bullet.mail.sp2.yahoo.com [98.139.91.218]) 26, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4H7drsP007378 26, 43 -- for {microscopy-at-microscopy.com} ; Tue, 17 May 2011 02:39:53 -0500 26, 43 -- Received: from [98.139.91.65] by nm20.bullet.mail.sp2.yahoo.com with NNFMP; 17 May 2011 07:39:53 -0000 26, 43 -- Received: from [98.139.91.42] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 17 May 2011 07:39:53 -0000 26, 43 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 17 May 2011 07:39:53 -0000 26, 43 -- X-Yahoo-Newman-Property: ymail-3 26, 43 -- X-Yahoo-Newman-Id: 125468.50882.bm-at-omp1042.mail.sp2.yahoo.com 26, 43 -- Received: (qmail 16948 invoked by uid 60001); 17 May 2011 07:39:52 -0000 26, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305617992; bh=frjB1VF4+S99ZKuFs4TsxatsBtm9iSzu7KS2vX5Q3NY=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=ZiJQ92JRBe/n8wBeHy6zr78uzib1KuFtWGJXo7+cilBxuwsQ4oj3Mtm7NkTH2RASpMiFn+TTDTzHr9JLQZERk9ugltObtJxSZhnr09D6/gcWV6PCjQFJ70wutriqRy3N8YHrvOItGw0dB08xiGsWarcwXaNxJ9ChAf1nvzSlnvA= 26, 43 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 26, 43 -- s=s1024; d=yahoo.com; 26, 43 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 26, 43 -- b=TkmXkiwXKmlnJ/cgysuBRF4bMvWRpdGiabH/KtU2ABjj6Dus6kYACdxhbaXwzdDwKH0sSC7q/lXc57lbglSD9avDuAWmIORbJFYa1ETnqbzP/nwcke8xAXd1WwkBCJ4fO7E4b45lqpvcsWdBEoMCt/BU6wvBBvzSDpoeeW5ncfk=; 26, 43 -- Message-ID: {528165.7754.qm-at-web110810.mail.gq1.yahoo.com} 26, 43 -- X-YMail-OSG: o2ufPzIVM1kDRb7fbKm5pXZ_A780FJ4JVd6Iz2psRA_EVQx 26, 43 -- 1uvVFOtscyrtD2A9fGMxyY4L1cGfTzvt_UbYr2QB3piyW3cCnf.lu25HhWw3 26, 43 -- HSwr_fn0hFqofhzEGV_SsaKXMQ6hVTN9_qoySdlyZ6GVTBV3hSKuJzIg3hrG 26, 43 -- cN2MgFVECodGFwINJT8SSk5etYj5cuxSOr8EoPLyTpmgqllxYYBlT5wHWdeN 26, 43 -- 3adsaOGiA6mOrTzrOrHg2HF8_HVn8fuU6MPAxXnQyq4bU2S4m8Zxf2lskMUi 26, 43 -- 5N9Kf.0NfKOIGpJ62oY15fckA3XcmnkhKJVfxRQIXXvlc1iW5DJjnJNao..d 26, 43 -- HhJCeRwam1AJ8E8wDgv6_4.aLZsepC3WqsHY2.4tbGw9GAFv2oR0yD5gW8At 26, 43 -- 2Lq0oQdfMk3_nNJkNTW0XsIb1ci38UqifRwijgLFbahK_g5qZ7zaCRfBPIfG 26, 43 -- hQQOnMNlITcvMJOWsf7nnFK3Eb6RtX8.Kr5IoXwlgrS6AeR7PdM452iFJEcq 26, 43 -- tAMPX2GTT3dsWE4l8bKe9AzusRgoSh_006o0kSrkYImc9q6OiaBdtGN8X151 26, 43 -- VCv41KmoR5t0Tt.4rk05iHKhpuR1YWBm6fUIFwQpwp73J71ZNO2p.hvnLdGq 26, 43 -- XxvNnewnMuZ5G8bSQY9ueeXl4xOgKgkLk0YJKGsAOEeGNfK7VqOGimo0uQoM 26, 43 -- k15HbUul9yt7B1OkQQRptaD79lBUdCJLIkeYomIJ9htheA8Nq4pNbNba_x6s 26, 43 -- c49.dg_7bxidRzBjSuJaE3u1sX7cA6eXXfChDgE2flLfzM49X3Kai10xUiKx 26, 43 -- zdPWrO2tFkv8kSg8P6Wvxi.I5liH2Ycg2ghYDW0ce 26, 43 -- Received: from [80.122.101.100] by web110810.mail.gq1.yahoo.com via HTTP; Tue, 17 May 2011 00:39:52 PDT 26, 43 -- X-Mailer: YahooMailRC/567 YahooMailWebService/0.8.111.303096 26, 43 -- References: {201105170202.p4H22HqV021001-at-ns.microscopy.com} 26, 43 -- Date: Tue, 17 May 2011 00:39:52 -0700 (PDT) 26, 43 -- From: Stephane Nizet {nizets2-at-yahoo.com} 26, 43 -- Subject: Re: [Microscopy] Re: Microscope service strategies - numbers 26, 43 -- To: microscopy-at-microscopy.com 26, 43 -- In-Reply-To: {201105170202.p4H22HqV021001-at-ns.microscopy.com} 26, 43 -- MIME-Version: 1.0 26, 43 -- Content-Type: text/plain; charset=utf-8 26, 43 -- Content-Transfer-Encoding: 8bit 26, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4H7drsP007378 ==============================End of - Headers==============================
There are several ways to do this depending on your time and type of equipment available. Quickest would be to just smear some yoghurt on a microscopy slide with a coverslip ontop and observe using good old fashioned light microscopy (DIC can give nice images here).
For SEM:
a) Just spread (thinly) on a stub and air dry - can be surprisingly effective but the coagulated milk may obscure many of the bugs. b) Use a traditional wet fixation and solvent dehydration - search "Kalab" for protocols (lots of papers from 1970's & 80's) which are modifications of typical glut/os fixation used in biology. Then sequential ethanol dehydration from 30 - 100%, followed by air drying or CPD via acetone. Dried pieces (2 - 3 mm cubed) can be fractured and mounted on a stub with carbon cement. c) Use cryo-SEM if possible as this gives the best preservation. I can send you an image or two showing the bacteria using this technique.
If you just want to see the bugs then a 1:1 dilution with water followed by mixing & gentle centrifugation will separate the milk solids, leaving bacteria suspended in the supernatant which can then be filtered.
Most half-decent fresh yoghurts should be teeming with bugs - choose a probiotic yoghurt to make sure as they require } 10exp9 viable organisms/g to be considered probiotic. You should see mixtures of long bacilli and short rods/cocci within a network of protein aggregates.
Regards Mark
Dr Mark A.E. Auty Manager, National Food Imaging Centre Food Chemistry and Technology Department Teagasc Food Research Centre Moorepark,Fermoy, Co. Cork, Ireland
28th & 29th September 2011 website: teagasc.ie/events/2011/cheese_symposium email: cheesesymposium2011-at-teagasc.ie
-----Original Message----- X-from: connellyps-at-nhlbi.nih.gov [mailto:connellyps-at-nhlbi.nih.gov] Sent: 16 May 2011 20:01 To: Mark Auty
Justin,
I once ran up Colby cheese for TEM and the bacteria were beautiful. If you can interest the student into switching I'd suggest cutting thin strips of cheese then break them and run the sample up for SEM or after CPD snap the strip into two. I'd think the bacteria should be visible along the fractured surface.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Does anyone have a protocol for going from straight-from-the-store yogurt to prepared SEM specimen? I have a student interested in seeing what the bacteria looks like. They are also trying to find out just how much of yogurt is, in fact, bacteria. Not being a biologist, I'm at a bit of a loss...
--Justin A. Kraft
==============================Original Headers============================== 19, 28 -- From connellyps-at-nhlbi.nih.gov Mon May 16 13:56:47 2011 19, 28 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4GIukcf010122 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 16 May 2011 13:56:47 -0500 19, 28 -- X-IronPortListener: Outbound_SMTP 19, 28 -- X-IronPort-Anti-Spam-Filtered: true 19, 28 -- X-IronPort-Anti-Spam-Result: Am4GAGdy0U2cKEcU/2dsb2JhbACbDIsLd6Z2g3KbSAKDLIJrBJARhEyDf4ZG 19, 28 -- Received: from unknown (HELO NIHHT01.nih.gov) ([156.40.71.20]) 19, 28 -- by nihxway3out.hub.nih.gov with ESMTP; 16 May 2011 14:56:45 -0400 19, 28 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT01.nih.gov 19, 28 -- ([156.40.71.20]) with mapi; Mon, 16 May 2011 14:56:38 -0400 19, 28 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 19, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 28 -- Date: Mon, 16 May 2011 14:56:37 -0400 19, 28 -- Subject: Re: [Microscopy] Re: SEM preparation of yogurt bacteria? 19, 28 -- Thread-Topic: [Microscopy] Re: SEM preparation of yogurt bacteria? 19, 28 -- Thread-Index: AcwT8VKY3ixSbfSqSye9dIhyNlC6zwACaj1c 19, 28 -- Message-ID: {C9F6EBA5.809D%connellyps-at-nhlbi.nih.gov} 19, 28 -- In-Reply-To: {201105161747.p4GHlPT8015891-at-ns.microscopy.com} 19, 28 -- Accept-Language: en-US 19, 28 -- Content-Language: en 19, 28 -- X-MS-Has-Attach: 19, 28 -- X-MS-TNEF-Correlator: 19, 28 -- acceptlanguage: en-US 19, 28 -- Content-Type: text/plain; charset="iso-8859-1" 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4GIukcf010122 ==============================End of - Headers============================== ------------------------------------------------------------------------------ Attention: This e-mail is privileged and confidential. If you are not the intended recipient please delete the message and notify the sender. Any views or opinions presented are solely those of the author. This email was scanned by Teagasc and has been certified virus free with the pattern file currently in use. This however cannot guarantee that it does not contain malicious content.
Tabhair aire: Ta an r-phost seo faoi phribhleid agus faoi run. Mura tusa an duine a bhi beartaithe leis an teachtaireacht seo a fhail, scrios e le do thoil agus cuir an seoltoir ar an eolas. Is leis an udar amhain aon dearcai no tuairimi a leiritear. Scanadh an r-phost seo le Teagasc agus deimhniodh go raibh se saor o vioras leis an bpatrunchomhad ata in usaid faoi lathair. Ni feidir a rathu leis seo afach nach bhfuil abhar mailiseach ann.
==============================Original Headers============================== 38, 27 -- From Mark.Auty-at-teagasc.ie Tue May 17 04:18:28 2011 38, 27 -- Received: from exchange02.teagasc.ie (exchange02.teagasc.ie [137.191.243.71]) 38, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4H9IRJ9025211 38, 27 -- for {microscopy-at-microscopy.com} ; Tue, 17 May 2011 04:18:27 -0500 38, 27 -- Received: from mail pickup service by exchange02.teagasc.ie with Microsoft SMTPSVC; Tue, 17 May 2011 10:18:26 +0100 38, 27 -- Message-ID: {D49F5C5008814A3CA8E190251A4F58C5-at-teagasc.net} 38, 27 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4841 38, 27 -- X-Mailer: Microsoft CDO for Exchange 2000 38, 27 -- Importance: normal 38, 27 -- Priority: normal 38, 27 -- Content-Class: urn:content-classes:message 38, 27 -- MIME-Version: 1.0 38, 27 -- Content-Type: text/plain; 38, 27 -- charset="us-ascii" 38, 27 -- Subject: RE: [Microscopy] SEM preparation of yogurt bacteria? 38, 27 -- Date: Tue, 17 May 2011 10:18:26 +0100 38, 27 -- In-Reply-To: {201105161901.p4GJ1Ffu017587-at-ns.microscopy.com} 38, 27 -- X-MS-Has-Attach: 38, 27 -- X-MS-TNEF-Correlator: 38, 27 -- Thread-Topic: [Microscopy] SEM preparation of yogurt bacteria? 38, 27 -- thread-index: AcwT+6LF/pDLHEpHRQiLf/fcTC8UNQAcOyIg 38, 27 -- References: {201105161901.p4GJ1Ffu017587-at-ns.microscopy.com} 38, 27 -- From: "Mark Auty" {Mark.Auty-at-teagasc.ie} 38, 27 -- To: {microscopy-at-microscopy.com} 38, 27 -- X-OriginalArrivalTime: 17 May 2011 09:18:26.0321 (UTC) FILETIME=[60B59810:01CC1473] 38, 27 -- Content-Transfer-Encoding: 8bit 38, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4H9IRJ9025211 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4HCxGYT012449 for {MicroscopyListserverArchive2-at-microscopy.com} ; Tue, 17 May 2011 07:59:16 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p4HCxG4w012447; Tue, 17 May 2011 07:59:16 -0500
Hi Rosemary, Your confocal sounds about right, although with only about a 25% increase over 10 years, I think you can count your blessings.
As for your ESEM, I should think the terms should look very much like your confocal contract. Are they claiming that all the break-downs were caused by mis-use and abuse? If that were the case, they shouldn't have covered the repairs even under warranty. On the other hand, if the failures were due to their poor design and construction, they certainly have a lot of nerve implying that your contract is being increased because of that. I'm also very surprised that parts are not covered. To me, that is not a service contract, particularly for a new instrument. It can be understandable for very old instruments, but should be reflected in the pricing.
Whoever the vendor is, I would certainly have a hard time recommending them to anyone, given the situation you describe.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: rosemary.white-at-csiro.au [mailto:rosemary.white-at-csiro.au] Sent: Monday, May 16, 2011 10:01 PM To: kenconverse-at-qualityimages.biz
Dear all,
Just to get some numbers here, how much is reasonable for a service contract? 10% of original cost of the instrument? 15% of cost?
However, for a new instrument (ESEM) from a different supplier, we were quoted about 15% of the original cost for a contract with 2 annual maintenance visits plus unlimited emergency callouts, but no coverage of replacement parts. For 2 maintenance visits and 2 emergency callouts but no parts the cost went down to about 8% of initial cost. The reason given for the cost was that we had many breakdowns in the 12 month warranty period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, computer fan blew up, etc, etc), and they had to factor this in. Considering the presumed reliability of SEMs compared to confocals (in my experience before this new instrument), and that no parts were covered, it seemed quite expensive.
Is the latter about what you'd expect to pay for a service contract?
Thanks, Rosemary White
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
==============================Original Headers============================== 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 2011 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4H1wX9q015257 13, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 -0500 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 13, 32 -- s=email; t=1305597514; x=1337133514; 13, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 13, 32 -- content-transfer-encoding; 13, 32 -- bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; 13, 32 -- b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv 13, 32 -- VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb 13, 32 -- +BsypGn+/TtdMUH; 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; 13, 32 -- d="scan'208";a="20957752" 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 13, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May 2011 11:58:32 +1000 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 13, 32 -- (TLS) id 8.3.159.2; Tue, 17 May 2011 11:58:32 +1000 13, 32 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 13, 32 -- Date: Tue, 17 May 2011 11:58:29 +1000 13, 32 -- Subject: Re: Microscope service strategies - numbers 13, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} 13, 32 -- To: {Microscopy-at-microscopy.com} 13, 32 -- Message-ID: {C9F81355.10B35%rosemary.white-at-csiro.au} 13, 32 -- Thread-Topic: Microscope service strategies - numbers 13, 32 -- In-Reply-To: {201105162121.p4GLLsvq019450-at-ns.microscopy.com} 13, 32 -- MIME-Version: 1.0 13, 32 -- Content-Type: text/plain; charset="ISO-8859-1" 13, 32 -- Content-Transfer-Encoding: 8bit 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4H1wX9q015257 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 28 -- From kenconverse-at-qualityimages.biz Tue May 17 07:59:16 2011 26, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 26, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4HCxFtC012444 26, 28 -- for {microscopy-at-microscopy.com} ; Tue, 17 May 2011 07:59:16 -0500 26, 28 -- X-Authority-Analysis: v=1.1 cv=2eRU7/Hz3VlqkdLxzKFKJPBwNf5HXLm+W19RdYpLufc= c=1 sm=0 a=qFnv_w0bjHcA:10 a=-CRmgG0JhlAA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=XnRPBOH4DihgOh83M_YA:9 a=pt6S-TXmAZJB4dK-i18A:7 a=jiObf9B0YAUA:10 a=5JUlSvKOtR0A:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=G91thX30KR8A:10 a=wi6guWX3a2tTbfmz:21 a=XBpoec4P42n-xxcn:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 26, 28 -- X-Cloudmark-Score: 0 26, 28 -- X-Originating-IP: 72.224.149.57 26, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1416] helo=Ken) 26, 28 -- by cdptpa-oedge03.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 26, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 26, 28 -- id 84/2B-03125-32172DD4; Tue, 17 May 2011 12:59:15 +0000 26, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 26, 28 -- To: {rosemary.white-at-csiro.au} , "MSA Listserver" {microscopy-at-microscopy.com} 26, 28 -- Subject: RE: [Microscopy] Re: Microscope service strategies - numbers 26, 28 -- Date: Tue, 17 May 2011 08:59:11 -0400 26, 28 -- Message-ID: {E9C2B6DDE3574F998FE24A44FCB9D732-at-Ken} 26, 28 -- MIME-Version: 1.0 26, 28 -- Content-Type: text/plain; 26, 28 -- charset="iso-8859-2" 26, 28 -- X-Priority: 3 (Normal) 26, 28 -- X-MSMail-Priority: Normal 26, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 26, 28 -- Importance: Normal 26, 28 -- Thread-Index: AcwUNkEb7efDY/ljTW6jHrG8pRn0NQAWhmew 26, 28 -- In-Reply-To: {201105170200.p4H20s4F018750-at-ns.microscopy.com} 26, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 26, 28 -- Content-Transfer-Encoding: 8bit 26, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4HCxFtC012444 ==============================End of - Headers==============================
I see that the MicroscopyListserver user's account "lingpine" has been used to spam the list. It was a valid account, but now is no longer trustworthy.
This account has been deleted and should no longer have access to the server.
Sorry for cluttering your Email.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory President Microscopy Society of America
Nestor, Please don't apologize! I'm having a hard time remembering the last time got any spam via the Listserver. I don't know how you do it, but thank you for doing such an outstanding job.
I sure wish I could automate my mailbox that well!
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Tuesday, May 17, 2011 12:17 PM To: kenconverse-at-qualityimages.biz
Colleagues;
I see that the MicroscopyListserver user's account "lingpine" has been used to spam the list. It was a valid account, but now is no longer trustworthy.
This account has been deleted and should no longer have access to the server.
Sorry for cluttering your Email.
Nestor Your Friendly Neighborhood SysOp
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science Division/Bldg 212 9700 S. Cass Ave Argonne, Illinois 60439 USA
Tel: 530-NES-TORZ (530-637-8679) has Voice Mail Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory President Microscopy Society of America
I was doing a backscattered imaging of a rock thin section today (in ESEM mode) which was mainly composed of K-feldpar, silicate matrix. There were a few square-shaped crystals that I thought to contain Fe-S, because of its very bright color (against a more grayish background). However, the spectrum gave me Na-Cl. Can salt crystals really appear bright in BSE Imaging?!
Thoughts?
Many thanks, Melina Miralles
==============================Original Headers============================== 7, 29 -- From mmiralles-at-pi.ac.ae Wed May 18 06:19:54 2011 7, 29 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4IBJrKQ030569 7, 29 -- for {microscopy-at-microscopy.com} ; Wed, 18 May 2011 06:19:54 -0500 7, 29 -- X-IronPort-AV: E=Sophos;i="4.65,231,1304280000"; 7, 29 -- d="scan'208";a="5212080" 7, 29 -- Received: from unknown (HELO pi-exf2.PI.AC.AE) ([192.168.2.13]) 7, 29 -- by mx1.pi.ac.ae with ESMTP; 18 May 2011 14:31:58 +0400 7, 29 -- Received: from pi-exm.PI.AC.AE ([192.168.2.10]) by pi-exf2.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 7, 29 -- Wed, 18 May 2011 15:21:26 +0400 7, 29 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 29 -- MIME-Version: 1.0 7, 29 -- Content-Type: text/plain; 7, 29 -- charset="us-ascii" 7, 29 -- x-cr-puzzleid: {8A3F07B6-B416-4C46-B290-FA92FA9A545B} 7, 29 -- x-cr-hashedpuzzle: AO7C A/50 BVgi BwI8 B8bR D+Yy Ee+K E/gE HSC9 H3ta H95/ IJmr Ib7W I5+b MGMT MWkk;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{8A3F07B6-B416-4C46-B290-FA92FA9A545B};bQBtAGkAcgBhAGwAbABlAHMAQABwAGkALgBhAGMALgBhAGUA;Wed, 18 May 2011 11:21:05 GMT;TgBBAEMAbAAgAGkAbgAgAEIAYQBjAGsAcwBjAGEAdAB0AGUAcgBlAGQAIABJAG0AYQBnAGkAbgBnAA== 7, 29 -- Content-class: urn:content-classes:message 7, 29 -- Subject: NACl in Backscattered Imaging 7, 29 -- Date: Wed, 18 May 2011 15:21:05 +0400 7, 29 -- Message-ID: {9FB1E16F7E29574A9095E74960E3C55D351143-at-pi-exm.PI.AC.AE} 7, 29 -- X-MS-Has-Attach: 7, 29 -- X-MS-TNEF-Correlator: 7, 29 -- Thread-Topic: NACl in Backscattered Imaging 7, 29 -- Thread-Index: AcwVTa3Os3sx3c0BS72XnmvxTRcQyg== 7, 29 -- From: "Melina Miralles" {mmiralles-at-pi.ac.ae} 7, 29 -- To: {microscopy-at-microscopy.com} 7, 29 -- X-OriginalArrivalTime: 18 May 2011 11:21:26.0873 (UTC) FILETIME=[BA463490:01CC154D] 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4IBJrKQ030569 ==============================End of - Headers==============================
Yes, salt will look bright in BSE because the Na is bound to Cl, not O, and Cl is even heavier than S. The crystals will also stand out in the image because they are sitting on the specimen surface, left behind by sweaty fingers. You've described exactly what one sees when sweat has left small salt crystals on an uncleaned specimen.
Best, Ellery
Ellery Frahm, Ph.D. Manager & Principal Analyst, Electron Microprobe Laboratory Research Associate, Department of Geology & Geophysics University of Minnesota - Twin Cities http://probelab.geo.umn.edu
==============================Original Headers============================== 5, 20 -- From frah0010-at-umn.edu Wed May 18 08:38:03 2011 5, 20 -- Received: from mta-a3.tc.umn.edu (mta-a3.tc.umn.edu [134.84.119.232]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4IDc3qu017107 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 18 May 2011 08:38:03 -0500 5, 20 -- Received: from [10.0.1.14] (c-75-72-178-217.hsd1.mn.comcast.net [75.72.178.217]) 5, 20 -- by mta-a3.tc.umn.edu (UMN smtpd) with ESMTP 5, 20 -- Wed, 18 May 2011 08:38:03 -0500 (CDT) 5, 20 -- X-Umn-Remote-Mta: [N] c-75-72-178-217.hsd1.mn.comcast.net [75.72.178.217] #+TS+AU+HN 5, 20 -- Content-Type: text/plain; charset=us-ascii 5, 20 -- Mime-Version: 1.0 (Apple Message framework v1082) 5, 20 -- Subject: Re: [Microscopy] NACl in Backscattered Imaging 5, 20 -- From: Ellery Frahm {frah0010-at-umn.edu} 5, 20 -- In-Reply-To: {201105181125.p4IBPsMu004970-at-ns.microscopy.com} 5, 20 -- Date: Wed, 18 May 2011 08:38:02 -0500 5, 20 -- Message-Id: {F8478F55-1E58-424B-B6E5-2C6F271E349A-at-umn.edu} 5, 20 -- References: {201105181125.p4IBPsMu004970-at-ns.microscopy.com} 5, 20 -- To: mmiralles-at-pi.ac.ae, microscopy-at-microscopy.com 5, 20 -- X-Mailer: Apple Mail (2.1082) 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4IDc3qu017107 ==============================End of - Headers==============================
I have seen similar behavior in Cl-rich areas in polished concrete. I have suspected it was due to charging but have not pursued the matter further to nail that down.
I also have seen increased BSE brightness from cathodoluminescent materials. I can confirm those by disconnecting the power to the illuminator on our chamber scope. I can see the beam scanning across those materials. I don't think that is the case for Cl, but will need to check.
Warren Straszheim
-----Original Message----- X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae] Sent: Wednesday, May 18, 2011 6:21 AM To: wesaia-at-iastate.edu
Hi,
I was doing a backscattered imaging of a rock thin section today (in ESEM mode) which was mainly composed of K-feldpar, silicate matrix. There were a few square-shaped crystals that I thought to contain Fe-S, because of its very bright color (against a more grayish background). However, the spectrum gave me Na-Cl. Can salt crystals really appear bright in BSE Imaging?!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kwylly-at-ppg.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kwylly-at-ppg.com Name: Kelly Wylly
Organization: PPG Industries, Inc.
Title-Subject: [Filtered] Cryo-SEM
Message: We are interested in finding a contract lab within the United States that could analyze liquid paint samples using cryo-SEM.
When I am asked to analyze a thin section, I have generally analyzed the free embedding plastic (at the edges of the section) to check for a Cl signal which regularly appears in cracks and holes in many sections - just to confirm the source when there appears to be 'nothing' at any B/C setting within the darkest BSE zones in the section.
Cheers,
FCM
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I have seen similar behavior in Cl-rich areas in polished concrete. I have suspected it was due to charging but have not pursued the matter further to nail that down.
I also have seen increased BSE brightness from cathodoluminescent materials. I can confirm those by disconnecting the power to the illuminator on our chamber scope. I can see the beam scanning across those materials. I don't think that is the case for Cl, but will need to check.
Warren Straszheim
-----Original Message----- X-from: mmiralles-at-pi.ac.ae [mailto:mmiralles-at-pi.ac.ae] Sent: Wednesday, May 18, 2011 6:21 AM To: wesaia-at-iastate.edu
Hi,
I was doing a backscattered imaging of a rock thin section today (in ESEM mode) which was mainly composed of K-feldpar, silicate matrix. There were a few square-shaped crystals that I thought to contain Fe-S, because of its very bright color (against a more grayish background). However, the spectrum gave me Na-Cl. Can salt crystals really appear bright in BSE Imaging?!
Thoughts?
Many thanks, Melina Miralles
==============================Original Headers============================== 19, 36 -- From FMonson-at-wcupa.edu Wed May 18 10:58:16 2011 19, 36 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) 19, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4IFwFkW003736 19, 36 -- for {microscopy-at-microscopy.com} ; Wed, 18 May 2011 10:58:16 -0500 19, 36 -- X-ASG-Debug-ID: 1305734295-0368bc4d0710dd50001-4CH8be 19, 36 -- Received: from WCU-XCH-06.PASSHE.LCL (wcu-xch-06.wcupa.edu [144.26.0.90]) by MX01.WCUPA.EDU with ESMTP id EouIFiPgQJhQAagp (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Wed, 18 May 2011 11:58:15 -0400 (EDT) 19, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 19, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.90 19, 36 -- X-ASG-Whitelist: Client 19, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 19, 36 -- WCU-XCH-06.PASSHE.LCL ([fe80::14b9:d9d5:4b99:2bb9%16]) with mapi id 19, 36 -- 14.01.0270.001; Wed, 18 May 2011 11:58:14 -0400 19, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 19, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 19, 36 -- Subject: RE: [Microscopy] NACl in Backscattered Imaging 19, 36 -- Thread-Topic: [Microscopy] NACl in Backscattered Imaging 19, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] NACl in Backscattered Imaging 19, 36 -- Thread-Index: AQHMFXRlV+lh0PXNZUuPpErWjrq2OA== 19, 36 -- Date: Wed, 18 May 2011 15:58:14 +0000 19, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C05FD80-at-WCU-XCH-03.PASSHE.LCL} 19, 36 -- References: {201105181400.p4IE0cqh013465-at-ns.microscopy.com} 19, 36 -- In-Reply-To: {201105181400.p4IE0cqh013465-at-ns.microscopy.com} 19, 36 -- Accept-Language: en-US 19, 36 -- Content-Language: en-US 19, 36 -- X-MS-Has-Attach: 19, 36 -- X-MS-TNEF-Correlator: 19, 36 -- x-originating-ip: [10.28.57.79] 19, 36 -- Content-Type: text/plain; charset="us-ascii" 19, 36 -- MIME-Version: 1.0 19, 36 -- X-Barracuda-Connect: wcu-xch-06.wcupa.edu[144.26.0.90] 19, 36 -- X-Barracuda-Start-Time: 1305734295 19, 36 -- X-Barracuda-Encrypted: AES128-SHA 19, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 19, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 19, 36 -- Content-Transfer-Encoding: 8bit 19, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4IFwFkW003736 ==============================End of - Headers==============================
Try using WinXray (from McGill), Electron Flight Simulator (SmallWorld), or similar to simply calculate the backscatter coefficient of the compounds in your sample.
This is a valuable tool.
Jim
} Date: Wed, 18 May 2011 06:30:58 -0500 } To: jquinn-at-ms.cc.sunysb.edu } From: mmiralles-at-pi.ac.ae } Subject: [Microscopy] NACl in Backscattered Imaging } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 16 -- From jquinn-at-ms.cc.sunysb.edu Wed May 18 12:13:36 2011 8, 16 -- Received: from mailrelay.stonybrook.edu (mrs.stonybrook.edu [129.49.1.206]) 8, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4IHDaCT021616 8, 16 -- for {microscopy-at-microscopy.com} ; Wed, 18 May 2011 12:13:36 -0500 8, 16 -- Received: from happy-b95c68896.ms.cc.sunysb.edu (ceas2064.matscieng.sunysb.edu [129.49.36.224]) 8, 16 -- by mailrelay.stonybrook.edu (8.14.2/8.14.2) with ESMTP id p4IHDYVF010720 8, 16 -- for {microscopy-at-microscopy.com} ; Wed, 18 May 2011 13:13:35 -0400 (EDT) 8, 16 -- Message-Id: {201105181713.p4IHDYVF010720-at-mailrelay.stonybrook.edu} 8, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 16 -- Date: Wed, 18 May 2011 13:13:18 -0400 8, 16 -- To: microscopy-at-microscopy.com 8, 16 -- From: Jim Quinn {jquinn-at-ms.cc.sunysb.edu} 8, 16 -- Subject: Fwd: [Microscopy] NACl in Backscattered Imaging 8, 16 -- Mime-Version: 1.0 8, 16 -- Content-Type: text/plain; charset="us-ascii" 8, 16 -- X-Brightmail-Tracker: AAAAAQAAAUA= ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both htsmith-at-syr.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: htsmith-at-syr.edu Name: Hunter Smith
Organization: Upstate Medical Center/ SUNY-ESF
Title-Subject: [Filtered] protein crystal embedding
Message: Just wondering if anyone could give some input or direction on embedding protein crystals. I have already embedded one set into L.R. resin, with one (1) dehydration step 50/50 (etoh/L.R.). Does anyone have tips for dehydration (or even if you need to run through dehydration steps with such small crystals) and the positioning of the crystals to 'float' in the L.R. resin? While embedding the I would fill the mold halfway with L.R. and insert the crystals. These crystals would float for a couple seconds, but once touched to reposition them, they would sink to the bottom. Is this going to make sectioning tough?
} Message: Just wondering if anyone could give some input or direction on } embedding protein crystals. I have already embedded one set into L.R. } resin, with one (1) dehydration step 50/50 (etoh/L.R.). } Does anyone have tips for dehydration (or even if you need to run } through dehydration steps with such small crystals) and the positioning } of the crystals to 'float' in the L.R. resin? } While embedding the I would fill the mold halfway with L.R. and insert } the crystals. These crystals would float for a couple seconds, but once } touched to reposition them, they would sink to the bottom. Is this going } to make sectioning tough?
depending on what you finally want to visualize, I would suggest another method - freeze-etching. Much faster, more reliable, for what you - probably - would like to see. have a look at this web-page (showing light microscopy and TEM of a freeze-etched crystal of a globular protein complex; of liposomes; and of a Euglena cell) - and decide. http://www.ch.tum.de/em/emlabor/methoden/tem-praep-b-gg.htm if you would prefer to have a translation of the text on this web-page, please contact either Sevil Weinkauf or myself directly.
Otherwise, I would recommend to do freeze-substitution (incl. fixation, if necessary), rather than dehydration/embedding at room temperature. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 7, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu May 19 01:24:18 2011 7, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4J6OHic032355 7, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 01:24:17 -0500 7, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 7, 25 -- by localhost (Postfix) with SMTP id 45C841E8E 7, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 08:24:12 +0200 (CEST) 7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 2D3B11E47 7, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 08:24:12 +0200 (CEST) 7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 25 -- with Novell_GroupWise; Thu, 19 May 2011 08:24:12 +0200 7, 25 -- Message-Id: {4DD4D3AB0200005400025196-at-gwsmtp1.uni-regensburg.de} 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 7, 25 -- Date: Thu, 19 May 2011 08:24:11 +0200 7, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 7, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 7, 25 -- Subject: protein crystal embedding 7, 25 -- References: {201105182350.p4INoaRw011837-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {201105182350.p4INoaRw011837-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=US-ASCII 7, 25 -- Content-Disposition: inline 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4J6OHic032355 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lisa.whitworth-at-okstate.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: lisa.whitworth-at-okstate.edu Name: Lisa Whitworth
Organization: Oklahoma State University
Title-Subject: [Filtered] TNT
Message: Has anyone looked at TNT particles using SEM? I'm looking for references, if possible.
An engineering graduate student would like to use the EBSD system which is in our Zeiss Ultraplus thermal FESEM to get some data from his sample. He thinks EBSD is the best solution. However, I have no expertise in EBSD analysis, so any help in this matter will be very much appreciated. I have placed two powerpoint slides in my dropbox for people to view. Please click on the link below or cut and paste the URL on your web browser to see the diagram and what he wants to do with his sample.
http://dl.dropbox.com/u/7737489/EBSD.ppt
Thanks in advance.
Soumitra
************************************************************ Soumitra Ghoshroy Director, Electron Microscopy Center Research Professor, Biology University of South Carolina 715 Sumter Street Columbia, SC 29208 803-777-7085 (office) 803-777-8908 (fax) http://www.emc.sc.edu
==============================Original Headers============================== 6, 30 -- From rintugr-at-gmail.com Thu May 19 13:39:59 2011 6, 30 -- Received: from mail-pv0-f169.google.com (mail-pv0-f169.google.com [74.125.83.169]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4JIdxrr010911 6, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 13:39:59 -0500 6, 30 -- Received: by pvc12 with SMTP id 12so1816760pvc.0 6, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 11:39:59 -0700 (PDT) 6, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 30 -- d=gmail.com; s=gamma; 6, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 6, 30 -- :content-type; 6, 30 -- bh=5L+hroCC1MvBYJuB7cPfghGsviAX9KyFpG/SG/5hlnM=; 6, 30 -- b=JT7ANogLtumQwNONWtitamGm6+fQBBZ0ZI9yIEkrMzCuDLd9kLGIyhn0OSazWEe0Da 6, 30 -- AjaQInyhFuYcEkCynIK7xgwFBd89h24ltelKQia4Q+4jQlkaSV6ZFITBz7pCQO49UunU 6, 30 -- EbE7CdaJT1yMUrJf4w0mPtByZ83TJiGEUVkzQ= 6, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 30 -- d=gmail.com; s=gamma; 6, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 6, 30 -- b=KE7JCfV6MnFvdsKDU4ZdeH8g0aibPGPtkxz5DW/MBi2xUEc39+hFBZSVhiV3vjN96f 6, 30 -- iBp/ckXnxSaHcMYFARP856j1VmYFgQPtBSwsZm+ei1ItsY9egKdEeHyWOYyMtdvdEp/g 6, 30 -- JTNXywqWiLs4Zzggi1AbzOverJ1jmnpUb7O+M= 6, 30 -- MIME-Version: 1.0 6, 30 -- Received: by 10.142.229.18 with SMTP id b18mr1991430wfh.201.1305830398893; 6, 30 -- Thu, 19 May 2011 11:39:58 -0700 (PDT) 6, 30 -- Received: by 10.142.221.17 with HTTP; Thu, 19 May 2011 11:39:58 -0700 (PDT) 6, 30 -- Date: Thu, 19 May 2011 14:39:58 -0400 6, 30 -- Message-ID: {BANLkTim2tdYHV8iyEiVb8SFV8_kP=sC8Mg-at-mail.gmail.com} 6, 30 -- Subject: EBSD question 6, 30 -- From: Soumitra Ghoshroy {rintugr-at-gmail.com} 6, 30 -- To: Microscopy-at-microscopy.com 6, 30 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
We are looking for parts for our H600 TEM. If you have any used or unused Hitachi H600 parts, please email us, we will be glad to pay small cost plus shipping for it.
Thank you.
Lisa
Glosuntech
==============================Original Headers============================== 7, 37 -- From lisa-at-glosuntech.com Fri May 20 00:56:33 2011 7, 37 -- Received: from omta2.brinkster.com (omta2.brinkster.com [65.182.109.101]) 7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4K5uW4g002399 7, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 May 2011 00:56:33 -0500 7, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 37 -- by omta2.brinkster.com (Postfix) with ESMTP id 489C01C1833D 7, 37 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 May 2011 22:56:31 -0700 (MST) 7, 37 -- X-Virus-Scanned: amavisd-new at 7, 37 -- Received: from omta2.brinkster.com ([127.0.0.1]) 7, 37 -- by localhost (omta2.brinkster.com [127.0.0.1]) (amavisd-new, port 10024) 7, 37 -- with ESMTP id Q6cqBTBhmjb3 for {Microscopy-at-Microscopy.Com} ; 7, 37 -- Thu, 19 May 2011 22:56:31 -0700 (MST) 7, 37 -- Received: from mta4.brinkster.com (unknown [65.182.109.83]) 7, 37 -- by omta2.brinkster.com (Postfix) with ESMTPS id 295621C181F4 7, 37 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 May 2011 22:56:31 -0700 (MST) 7, 37 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 7, 37 -- by mta4.brinkster.com (Postfix) with ESMTP id 1DA1E281406 7, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 May 2011 01:56:32 -0400 (EDT) 7, 37 -- X-Virus-Scanned: amavisd-new at mta4.brinkster.com 7, 37 -- Received: from mta4.brinkster.com ([127.0.0.1]) 7, 37 -- by localhost (mta4.brinkster.com [127.0.0.1]) (amavisd-new, port 10024) 7, 37 -- with ESMTP id zHxgWn6+Kb1q for {Microscopy-at-Microscopy.Com} ; 7, 37 -- Fri, 20 May 2011 01:56:31 -0400 (EDT) 7, 37 -- Received: from mail10a.brinkster.com (mail10a.brinkster.com [10.0.6.217]) 7, 37 -- by mta4.brinkster.com (Postfix) with ESMTP id CC1C7281318 7, 37 -- for {Microscopy-at-Microscopy.Com} ; Fri, 20 May 2011 01:56:31 -0400 (EDT) 7, 37 -- Date: Fri, 20 May 2011 01:56:30 -0400 (EDT) 7, 37 -- From: lisa-at-glosuntech.com 7, 37 -- To: Microscopy {Microscopy-at-Microscopy.Com} 7, 37 -- Message-ID: {13337219.7132.1305870990095.JavaMail.root-at-mail10a.brinkster.com} 7, 37 -- In-Reply-To: {19748197.7092.1305866822063.JavaMail.root-at-mail10a.brinkster.com} 7, 37 -- Subject: Hitachi H600 TEM Parts needed 7, 37 -- MIME-Version: 1.0 7, 37 -- Content-Type: text/plain; charset=utf-8 7, 37 -- Content-Transfer-Encoding: 7bit 7, 37 -- X-Originating-IP: [75.140.98.215] 7, 37 -- X-Mailer: Zimbra 6.0.6_GA_2332.RHEL4 (ZimbraWebClient - IE8 (Win)/6.0.6_GA_2324.RHEL5) ==============================End of - Headers==============================
If he really needs to determine the individual crystallographic orientation(s) of areas only about 5µm across, EBSD would indeed be the best solution (actually the only one I know), but ...
I have encountered EBSD in two applications (in metals): 1) The determination of the orientation density function of the crystallites as a complement to an XRD texture analysis. 2) The determination of the 'misalignment' between the lattice structures of adjoining crystallites to calculate the interface energy/strength.
Since the plates in his sample are separated by a secondary phase, it cannot be case 2), and in case 1) he would have to map a lot of plates, not just a few.
Regards, Jens
Jens Lietzau Electron Microscopy, X-Ray Diffraction & Spectroscopy GKN Driveline Research & Product Development Centre Hauptstrasse 130, 53797 Lohmar, Germany
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Colleagues,
An engineering graduate student would like to use the EBSD system which is in our Zeiss Ultraplus thermal FESEM to get some data from his sample. He thinks EBSD is the best solution. However, I have no expertise in EBSD analysis, so any help in this matter will be very much appreciated. I have placed two powerpoint slides in my dropbox for people to view. Please click on the link below or cut and paste the URL on your web browser to see the diagram and what he wants to do with his sample.
http://dl.dropbox.com/u/7737489/EBSD.ppt
Thanks in advance.
Soumitra
************************************************************ Soumitra Ghoshroy Director, Electron Microscopy Center Research Professor, Biology University of South Carolina 715 Sumter Street Columbia, SC 29208 803-777-7085 (office) 803-777-8908 (fax) http://www.emc.sc.edu
--------------------------------------------------------------------------  GKN Driveline International GmbH, Hauptstr. 130, 53797 Lohmar Sitz der Gesellschaft: Lohmar - Amtsgericht Siegburg HR B 2117 Geschäftsführung: Karl-Friedrich Roth, Dieter Thiede -------------------------------------------------------------------------- This e-mail and any attachments transmitted with it contain information which is confidential, intended solely for the addressee(s) and which may also be privileged or exempt from disclosure under applicable law. If you are not the addressee(s), or have received this e-mail in error, please notify the sender immediately, delete it from your system and do not copy, print, disclose or use any part of it or its attachments. Internet communications are not guaranteed to be secure or free of any virus. The GKN Group does not accept liability for any loss or damage arising in any way from changes to this e-mail or its attachments which may occur in transmission due to network, machine or software failure or manufacturer or operator error, or from unauthorised access or interference with internet communications by any third party or from the transmission of any viruses. Any opinion or other information in this e-mail or its attachments that does not relate to the business of the GKN Group or any part of it is personal to the sender and is not given or in any way endorsed by the GKN Group or any part of it.
==============================Original Headers============================== 17, 28 -- From Jens.Lietzau-at-gkndriveline.com Fri May 20 01:44:18 2011 17, 28 -- Received: from na3sys009aog101.obsmtp.com (na3sys009aog101.obsmtp.com [74.125.149.67]) 17, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4K6iH9u019064 17, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 01:44:17 -0500 17, 28 -- Received: from Mail4.gkndriveline.com ([194.76.60.27]) by na3sys009aob101.postini.com ([74.125.148.12]) with SMTP 17, 28 -- ID DSNKTdYNwODqWuIoNt/epYDQ/fSZuDe1d4kE-at-postini.com; Thu, 19 May 2011 23:44:17 PDT 17, 28 -- Received: from MAILSRV13.DL.ADD.INT ([10.150.16.147] RDNS failed) by Mail4.gkndriveline.com with Microsoft SMTPSVC(6.0.3790.4675); 17, 28 -- Fri, 20 May 2011 08:44:15 +0200 17, 28 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 17, 28 -- Content-class: urn:content-classes:message 17, 28 -- MIME-Version: 1.0 17, 28 -- Content-Type: text/plain; 17, 28 -- charset=utf-8 17, 28 -- Disposition-Notification-To: "Jens Lietzau" {Jens.Lietzau-at-gkndriveline.com} 17, 28 -- Subject: RE: EBSD question 17, 28 -- Date: Fri, 20 May 2011 08:44:15 +0200 17, 28 -- Message-ID: {FF86EB43A37DBD4CBB64E85F075AD27402954350-at-MAILSRV13.DL.ADD.INT} 17, 28 -- In-Reply-To: {201105191848.p4JImnFj026894-at-ns.microscopy.com} 17, 28 -- X-MS-Has-Attach: 17, 28 -- X-MS-TNEF-Correlator: 17, 28 -- Thread-Topic: EBSD question 17, 28 -- Thread-Index: AcwWVWeOHc4U/S4wT6O5svBXYKbi7QAYTVXg 17, 28 -- References: {201105191848.p4JImnFj026894-at-ns.microscopy.com} 17, 28 -- From: "Jens Lietzau" {Jens.Lietzau-at-gkndriveline.com} 17, 28 -- To: {Microscopy-at-microscopy.com} 17, 28 -- X-OriginalArrivalTime: 20 May 2011 06:44:15.0781 (UTC) FILETIME=[56327550:01CC16B9] 17, 28 -- Content-Transfer-Encoding: 8bit 17, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4K6iH9u019064 ==============================End of - Headers==============================
We recently bought a second-hand JEOL 6301F which came with an Olympus ADDA II imaging system controller and PCI card but not the Dongle and Software. I have been trying to get a dongle and software licence from Olympus but they are are not interested in supplying the original software (AnalySIS) and disks as they want us to buy Scandium which seems to cost the earth for just capturing images (£3000) especially as the hardware it is not the most current model. My questions are has anyone:
1. Got a spare software version and licence they would be willing to part with? 2. Got experience grabbing images using this particular hardware but by using some other piece of software. 3. Got any advice on the Scandium software, is it worth the £3k for the basic package? 4. Have any suggestions for an alternative image capture system of comparable price? We do not need to do anything else except grab images. We do not need any sort of column control. Any advice would be gratefully received.
Regards John University of Bath, UK
==============================Original Headers============================== 5, 33 -- From john.mitchels-at-gmail.com Fri May 20 03:55:54 2011 5, 33 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 5, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4K8trHE004734 5, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 03:55:54 -0500 5, 33 -- Received: by pzk4 with SMTP id 4so2154861pzk.0 5, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 01:55:52 -0700 (PDT) 5, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 33 -- d=gmail.com; s=gamma; 5, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 5, 33 -- :content-type:content-transfer-encoding; 5, 33 -- bh=WGm4P7ezzNMZbZ1wfLlh9qBDVhq8loShwFF5fntVB78=; 5, 33 -- b=Wk/t2KY1jSNp0D80e9gYTV6CFZmHn8QGqUCvIwiBHtX7iIqp2d9GyACOFFL5lPACFX 5, 33 -- HlSknZwlTZxWmjhBijKmypZU3U7DRVBilFJEkbdDoGCD0MA5VrWSwa8j0CLv6GsDQQlt 5, 33 -- 0K9VEvbz9NY6RVYyMswJR/dtetUfy0gm2Aao0= 5, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 5, 33 -- d=gmail.com; s=gamma; 5, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 5, 33 -- :content-transfer-encoding; 5, 33 -- b=NYCU9Iw4eHv1Udphx03hiuBaIkEoiq5x7G6I64leSsSqUapikY7WpELjfO4Co2PH9+ 5, 33 -- gFfGJv7lJhgrIQVJ2ojv6bK1mLqCtB1DsI3ux7awkdKi6YoN6ZPu609ILkIVgUD/VKp3 5, 33 -- yNy9Ngk6OFK3WE64tO5Yh2f7jphgw65sZ8ZsE= 5, 33 -- MIME-Version: 1.0 5, 33 -- Received: by 10.68.17.129 with SMTP id o1mr6597480pbd.178.1305881752122; Fri, 5, 33 -- 20 May 2011 01:55:52 -0700 (PDT) 5, 33 -- Received: by 10.68.60.163 with HTTP; Fri, 20 May 2011 01:55:51 -0700 (PDT) 5, 33 -- Date: Fri, 20 May 2011 09:55:51 +0100 5, 33 -- Message-ID: {BANLkTin-PrXkJh_8ANkV8vWXRX8RjZZmFw-at-mail.gmail.com} 5, 33 -- Subject: Olympus ADDA II Question 5, 33 -- From: John Mitchels {john.mitchels-at-gmail.com} 5, 33 -- To: Microscopy-at-microscopy.com 5, 33 -- Content-Type: text/plain; charset=ISO-8859-1 5, 33 -- Content-Transfer-Encoding: 8bit 5, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4K8trHE004734 ==============================End of - Headers==============================
Not TNT and no literature references, but I look at explosive powders (RDX, HMX, HNS, PETN etc.) in the SEM regularly. Aluminum stub for heat dissipation, gold sputter coat to limit charging and enhance secondary electron yield, 3-5 KV accelerating voltage, nice images. What kind of details are you looking for?
Regards, Andrew Werner Chief Metallurgist, Perforating Schlumberger Reservoir Completions 14910 Airline Road Rosharon, TX 77583
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, May 19, 2011 12:40 PM To: Andrew Werner
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lisa.whitworth-at-okstate.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: lisa.whitworth-at-okstate.edu Name: Lisa Whitworth
Organization: Oklahoma State University
Title-Subject: [Filtered] TNT
Message: Has anyone looked at TNT particles using SEM? I'm looking for references, if possible.
Chris, You should also consider that the company may advertise 25% savings, but on advanced equipment, they will probably back off to 10-15%, since one unreliable instrument can suck up their pool of money faster than their profit margin can afford.
If can you show me an equipment service underwriter's quote for 25% less than list for a well-equipped confocal or electron microscope, you'll definitely have my interest. Of course this depends on the size of the company's pool of contracts.
Regards, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan Molecular, Cellular, and Developmental Biology Ann Arbor, Michigan USA
-----Original Message----- X-from: Christopher.Gilpin-at-utsouthwestern.edu [mailto:Christopher.Gilpin-at-utsouthwestern.edu] Sent: Monday, May 16, 2011 3:24 PM To: Sobocinski, Gregg
All, There is a new option that is floating around the University of Texas system. An independent company has signed a deal with UT system to save everyone 25% on their service contracts (not just EMs - a whole range of equipment, as long as it is a parts and labor contract). They get you to move to an "on demand" arrangement with your existing service provider. They pay all the bills and take the chance that you will spend less than a full price contract. Of course there is the issue with how service providers prioritize contract versus on-demand customers. This company will freeze the cost for 4 years and as part of the arrangement with UT system (with qualifying contracts and equipment) will not decline coverage.
I am tempted but don't like the idea of having less priority when I make a service call.
Regards
Chris
Christopher J Gilpin Ph.D. Assistant Professor Director, Molecular and Cellular Imaging Facility K1.A04 UT Southwestern Medical Center at Dallas 5323 Harry Hines Boulevard Dallas, TX 75390-9039 Phone +1 214 648 2827
-----Original Message----- X-from: raharris-at-ucdavis.edu [mailto:raharris-at-ucdavis.edu] Sent: Monday, May 16, 2011 12:38 PM To: Christopher Gilpin
I have been following this thread intermittently so I do not know if what I am suggesting has been mentioned. Also, it is a little off topic so please forgive me; I am not trying to high-jack the thread but just to insert a comment. In my experience, if the administration of your school or university is looking for a way to indirectly close down a program, this is one of the strategies they will use. I have seen it happen many times at the university where I have worked since 1979.
Good luck,
Rick A. Harris
______________________________________ X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com] Sent: Monday, May 16, 2011 1:43 AM To: Rick A Harris
Basically what you are saying is that people with more money are happier. This is quite an evidence, isn't it? I don't think people leave a service contract when they don't have to.
Regards,
Stephane
----- Original Message ---- X-from: "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} To: nizets2-at-yahoo.com Sent: Fri, May 13, 2011 3:25:39 PM
Valery, Very well stated. The only thing I might add is that the customer with a service contract is guaranteed certain things, such as preventive maintenance, operation to specification, perhaps even guaranteed maximum down-time. The one-off customer has none of this. The service organization is contractually bound to the contract customer. Period. Yes, the one off cash is nice, but the regular customers are far more valuable in the long run. They often are happier customer, also.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 13, 2011 8:59 AM To: kenconverse-at-qualityimages.biz
Dave's message below compelled me to explain a difference between service contract and one-off service call from the vendor's perspective.
For most of FIB/SEM equipment service providers, both small and large, equipment service is a business activity; it must be profitable or could not continue. Delivering service and making it possible also costs money: most of users do not realize how enormous are the aggregated expenses of labor, travel, sourcing parts, re-designing or substituting obsolete components, warehousing, bean counting, etc...
When you buy a service contract from the certain provider, it not just gives you reliable support with predictable response time, but also gives that service organization reliable stream of revenue and predictable work load. Stability allows to plan and structure business, retain and maintain necessary resources, source and stock parts, etc.
If customer goes off service contract then situation becomes completely unpredictable: he/she may call in for service, but also may do self-service, hire internal service person, or do whatever else. In such situation managers of the service organization have no other choice but to prepare for the worse and assume that there will be no service calls from this customer. To insure survival of the organization, cost-saving measures follow immediately: engineers are laid off, facilities are closed or downsized, parts are not procured and not stored, and so forth. These measures are inevitable - otherwise service organization simply could not continue to exist. Reduced resources will usually be matched to the needs of contract-paying customers, with little to no spare capacity.
When odd service call comes from the customer without the service contract, it is treated as such - one-off, single-case business, which may never return. Yes, it is nice to get some extra revenue, but (already reduced) resources of engineering time and parts will be given to this call only if and when all the needs of service-contract customers are taken care of, and not a split second earlier.
The bottom line is that service organization simply can't provide more service then what it paid for, and overall level of service users receive will always degrade in one way or another, as the service revenue decreases.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We went off contract last year and had to wait over 4 months for a service. The engineer said that if it had been a breakdown the delay would have been the same. } } Dave } } -----Original Message----- } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } Sent: 12 May 2011 17:50 } To: David Patton } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Friends: } } We are caught on the horns of a dilemma. For nearly forty years, Delta College has trained EM technicians for positions in academia and industry. For most of those forty years our instruments, now numbering 3 TEM's, 4, SEM's, an AFM and an FIB, have been covered by service contracts. Our instruments are used all day, every day for student training. } } As you may have heard, funding for California schools is getting so } tight that the bean counters are turning to drastic measures to make ends meet. Among pressures put on us is a requirement that we enroll at least 20 students/class, nearly impossible in our advanced, hand-on classes. In addition, the administration is now reluctant to authorize service contracts for our instruments. } } Many or our microscopes are older and require more repairs due to } their constant use as training instruments. We have received good service from our scope vendor, but it is expensive, mostly because we have so many instruments. The admin. people only look at the bottom line and when they see the big number, they go ballistic.
} } We are afraid that if we drop our current service contracts that the manufacturer will not pick them up if we want to come back after a year off their service. We are afraid that if we go to pay as you go with the manufacturer, we will be low in their priority list, something not good with so many students depending on microscope time to finish projects. We are afraid that if we can find a cheaper, independent service provider, parts will be hard to get and the price of the parts would be in addition to the cost of the service agreement, whereas parts are included in our current contracts. } } This is getting long. Bottom line is we are asking for any advice or suggestions about how to approach this. I have heard all the horror stories about 3rd party insurance plans, etc. Are those stories true? Is it true that vendors move you to the bottom of the list without a contract? What is your experience if ever faced by this kind of situation? What kind of strategy has worked, or not? } } Hoping for a miracle, } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, 34 } -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4CGlQmx017267 } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 11:47:26 -0500 } 13, 34 -- Received: from mailin.deltacollege.edu } (localhost.localdomain [127.0.0.1]) } 13, 34 -- by localhost (Email Security Appliance) with SMTP id BA1EEABF5A8_DCC0F1DB } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from sunspot.sjdccd.cc.ca.us } (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 13, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 57311ABE27A_DCC0F1DF } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 16:47:25 +0000 (GMT) } 13, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 13, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 13, 34 -- ESMTP id LL3DB000.PFX for {Microscopy-at-microscopy.com} ; Thu, 12 } 13, 34 -- May 2011 09:47:24 -0700 } 13, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 268157A3841C } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:25 -0700 (PDT) } 13, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 13, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 13, 34 -- with ESMTP id pHJsHJArXDuA for {Microscopy-at-microscopy.com} ; } 13, 34 -- Thu, 12 May 2011 09:47:21 -0700 (PDT) } 13, 34 -- Received: from vstewart-green.deltacollege.edu (vstewart-green.deltacollege.edu [172.20.2.131]) } 13, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 13E637A3842F } 13, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2011 09:47:16 -0700 (PDT) } 13, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 13, 34 -- } Content-Type: text/plain; charset=us-ascii 13, 34 -- Subject: } Microscope service strategies 13, 34 -- Date: Thu, 12 May 2011 } 09:47:15 -0700 13, 34 -- Message-Id: {67D01FE5-B154-4495-93DF-55ADE91114BF-at-deltacollege.edu} } 13, 34 -- To: Microscopy-at-microscopy.com 13, 34 -- Mime-Version: 1.0 } (Apple Message framework v1084) 13, 34 -- X-Mailer: Apple Mail } (2.1084) 13, 34 -- Content-Transfer-Encoding: 8bit 13, 34 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4CGlQmx017267 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 23, 46 -- From David.Patton-at-uwe.ac.uk Fri May 13 04:58:55 2011 23, 46 } -- Received: from AM1EHSOBE004.bigfish.com (am1ehsobe004.messaging.microsoft.com [213.199.154.207]) } 23, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4D9wsRc031818 } 23, 46 -- for {Microscopy-at-microscopy.com} ; Fri, 13 May 2011 04:58:54 -0500 } 23, 46 -- Received: from mail92-am1-R.bigfish.com (10.3.201.250) by } 23, 46 -- AM1EHSOBE004.bigfish.com (10.3.204.24) with Microsoft SMTP Server id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:50 +0000 23, 46 -- } Received: from mail92-am1 (localhost.localdomain [127.0.0.1]) by } 23, 46 -- mail92-am1-R.bigfish.com (Postfix) with ESMTP id } C44681BB808D; Fri, 13 May } 23, 46 -- 2011 09:58:50 +0000 (UTC) } 23, 46 -- X-SpamScore: -14 } 23, 46 -- X-BigFish: VPS-14(zz7f0R542Mzz1202hz31iz8275bhz2fh2a8h668h839h62h) } 23, 46 -- X-Spam-TCS-SCL: 1:0 } 23, 46 -- X-Forefront-Antispam-Report: CIP:164.11.127.42;KIP:(null);UIP:(null);IPVD:NLI;H:egen-hub02.campus.ads.uwe .ac.uk;RD:none;EFVD:NLI } 23, 46 -- Received-SPF: pass (mail92-am1: domain of uwe.ac.uk } designates 164.11.127.42 as permitted sender) client-ip=164.11.127.42; envelope-from=David.Patton-at-uwe.ac.uk; helo=egen-hub02.campus.ads.uwe.ac.uk ;ds.uwe.ac.uk ; } 23, 46 -- Received: from mail92-am1 (localhost.localdomain } [127.0.0.1]) by mail92-am1 } 23, 46 -- (MessageSwitch) id 1305280730406851_26673; Fri, 13 May 2011 09:58:50 +0000 } 23, 46 -- (UTC) } 23, 46 -- Received: from AM1EHSMHS001.bigfish.com (unknown } [10.3.201.242]) by } 23, 46 -- mail92-am1.bigfish.com (Postfix) with ESMTP id 56BCF1004B; Fri, 13 May 2011 } 23, 46 -- 09:58:50 +0000 (UTC) } 23, 46 -- Received: from egen-hub02.campus.ads.uwe.ac.uk } (164.11.127.42) by } 23, 46 -- AM1EHSMHS001.bigfish.com (10.3.207.101) with Microsoft SMTP Server (TLS) id } 23, 46 -- 14.1.225.22; Fri, 13 May 2011 09:58:49 +0000 23, 46 -- } Received: from EGEN-MBX02.campus.ads.uwe.ac.uk ([fe80::7dcf:a903:5a0:acc3]) by } 23, 46 -- egen-hub02.campus.ads.uwe.ac.uk ([164.11.127.42]) with } mapi; Fri, 13 May 2011 } 23, 46 -- 10:57:55 +0100 } 23, 46 -- From: David Patton {David.Patton-at-uwe.ac.uk} 23, 46 -- To: } "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} } 23, 46 -- Date: Fri, 13 May 2011 10:57:55 +0100 23, 46 -- Subject: RE: } [spam]* [Microscopy] Microscope service strategies 23, 46 -- } Thread-Topic: [spam]* [Microscopy] Microscope service strategies 23, } 46 -- Thread-Index: AcwQxKwIp6eBV2VtSmCXiSInmQDlSgAjsewA } 23, 46 -- Message-ID: {A169BAD2C2DC6D418270CDC03DF5CDF437E7C868DE-at-EGEN-MBX02.campus.ads .uwe.ac.uk} } 23, 46 -- References: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- In-Reply-To: {201105121650.p4CGo36I022302-at-ns.microscopy.com} } 23, 46 -- Accept-Language: en-US, en-GB 23, 46 -- Content-Language: } en-US 23, 46 -- X-MS-Has-Attach: } 23, 46 -- X-MS-TNEF-Correlator: } 23, 46 -- acceptlanguage: en-US, en-GB 23, 46 -- Content-Type: } text/plain; charset="us-ascii" } 23, 46 -- MIME-Version: 1.0 } 23, 46 -- X-OriginatorOrg: uwe.ac.uk } 23, 46 -- Content-Transfer-Encoding: 8bit 23, 46 -- } X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4D9wsRc031818 } ==============================End of - Headers============================== }
==============================Original Headers============================== 9, 46 -- From vray-at-partbeamsystech.com Fri May 13 07:56:45 2011 9, 46 -- Received: from nm11-vm0.bullet.mail.sp2.yahoo.com (nm11-vm0.bullet.mail.sp2.yahoo.com [98.139.91.240]) 9, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4DCuiFa017690 9, 46 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 07:56:44 -0500 9, 46 -- Received: from [98.139.91.65] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [98.139.91.44] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- Received: from [127.0.0.1] by omp1044.mail.sp2.yahoo.com with NNFMP; 13 May 2011 12:56:43 -0000 9, 46 -- X-Yahoo-Newman-Id: 926684.30124.bm-at-omp1044.mail.sp2.yahoo.com 9, 46 -- Received: (qmail 24298 invoked from network); 13 May 2011 12:56:43 -0000 9, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1305291403; bh=i3t6rce7EUD6aDGPvzQA/rQ817rrGLyBWYws3CjYnFc=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date: X-from:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:Reference s: In-Reply-To:Content-Type:Content-Transfer-Encoding; b=LMDPdpKtj5mgnWOEaGVEKmUjfqFNLAvYPwnPka7kC4crghDG1dZNm6HXxw/G3RHedIkiWRDbZm 2+xDJh3XLkULLpUSnz0zLFj/os6cSNM+oI4/vpORPBheuT6YFQKu07bBlmoYk3ZG6Yj4a7cO 2+5XVi jSeh727RbKIgzNj4VrHQ4= 9, 46 -- Received: from [192.168.1.111] (vray-at-75.69.242.43 with plain) 9, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 13 May 2011 05:56:43 -0700 PDT 9, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 46 -- X-YMail-OSG: VtIzVvAVM1mOzDedm8J49KEXsbmPJsSdstcYcJjs_TEHrRm 9, 46 -- nqQj8MH_PkLg6UunVzpZJLtBsQcxDstWyfaf_FmuRQDIBzzvdhmVqgVcxdcw 9, 46 -- O47RduhMqWKpL3HCAdD7kiIA44BQT85ASoYN6X3tx42O8mHYmKJy8m3JAIFc 9, 46 -- bi5tbpuf3Id8Aullt7ksUSoleokcH73mb28nCow482Z3UEF1QjODThuYTkkg 9, 46 -- sQvRkVrq8fMts2ViAvaZl8434_9Lo2j6kODcLhq3HHyc5.5sG9Isbd4qRQWP 9, 46 -- iWhgaIXWqBJOdzA1Iw8Pm9yTRgxa2q5wA2onOlfU7Kqed1sVYd75.7mYhpPz 9, 46 -- ImfQ4aUMdwiQc6ktmwOOQDbE.jruIMERsuUWLPUWLIJTfRUs5Wg2.fxa0AhL 9, 46 -- JuQW80e7rNrh6NDXqLdqKOI4rVD_b.ekebopJBaj7fSTmAj6Sm_ZZ.N.TcnA 9, 46 -- 1y1Lqcqvr8fWjA89Ye4zSHpiLRjrsELrOQ4AbEkArggCS8kfzjhFS7QnwIxZ 9, 46 -- 7VNnzb3gkJ.qUXONbZXTAy2XID4uXmavh1qdCKL9O1YhfiGFV9HsPJZ_rQ.W 9, 46 -- sivH7RLmKjgXX6EssVcBa6jU5YRkJBq2mY9p7BuEWCQYRGYFYiCtLYfXcLv6 9, 46 -- wFcAiaRPmMIvpvkRimRBftERUbFA5jvUVw26AdFzLKGV8WSndtfOB4ekBR4E 9, 46 -- roBTLyN7VMVSJ6oIoswEfEfd6KGoinoJmt_0CHYBYE2qjEPuHnqBTGLzBrb4 9, 46 -- _2NkXrygVBuyEi9ig87T9nQAR1k.JCOG9zw1q.XwB4sCflAnLUrhWBK3MxyU 9, 46 -- U9Zad823Sxov6.Qj_gldIdBeHLH2fo1jyXIpM7QOb8PfXXj3_WTyWrwG0y1f 9, 46 -- 24FYtpJdkwflewdarDg5HPcpIeIE0bqAJ4zatw71XkrWJZitIQlXZSCmxubM 9, 46 -- XJ301_BGbOeSpadeuEhBGFTXwlRRdvliOrpaNMVhIKpIO6cV3sukibxvR4kQ 9, 46 -- - 9, 46 -- X-Yahoo-Newman-Property: ymail-3 9, 46 -- Message-ID: {4DCD2A8B.6030903-at-partbeamsystech.com} 9, 46 -- Date: Fri, 13 May 2011 08:56:43 -0400 9, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 46 -- Reply-To: vray-at-partbeamsystech.com 9, 46 -- Organization: PBS&T 9, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 9, 46 -- MIME-Version: 1.0 9, 46 -- To: David.Patton-at-uwe.ac.uk 9, 46 -- CC: microscopy-at-microscopy.com 9, 46 -- Subject: Re: [Microscopy] RE: [spam]* Microscope service strategies 9, 46 -- References: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- In-Reply-To: {201105130959.p4D9xxRN000709-at-ns.microscopy.com} 9, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 46 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Fri May 13 08:22:05 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4DDM5G5001569 20, 28 -- for {microscopy-at-microscopy.com} ; Fri, 13 May 2011 08:22:05 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=2lq4+qobG88tJFkiXmxSLStFAwyj+PriBwHB+7DDebs= c=1 sm=0 a=d6ZFszS330cA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=DahTZl4gAAAA:8 a=Zx37jsudAAAA:8 a=_CgzANL9AAAA:8 a=-gyhParZAAAA:8 a=hCCmtxs2AAAA:8 a=yMhMjlubAAAA:8 a=CjxXgO3LAAAA:8 a=15LFL-canmR1PGpTlQ8A:9 a=YOHmFmhgio4DZwt2t8EA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=U5vkcOzocHIA:10 a=brlFRoIUItEA:10 a=RO2JPw24U6UA:10 a=PmYly_lw2igA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=f96-IYUahzkA:10 a=G91thX30KR8A:10 a=rC2wZJ5BpNYA:10 a=yUsKKQj0Tui2F24u:21 a=NZ21XijlkcsDGYSt:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2310] helo=Ken) 20, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id AC/77-17273-C703DCD4; Fri, 13 May 2011 13:22:05 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {vray-at-partbeamsystech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] [spam]* Microscope service strategies 20, 28 -- Date: Fri, 13 May 2011 09:21:53 -0400 20, 28 -- Message-ID: {784D2C0EDDA74302903AA04B2F8E61BF-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- Importance: Normal 20, 28 -- Thread-Index: AcwRbZETKQmlIEYgQWGwqB32vBi+0AAAjrwg 20, 28 -- In-Reply-To: {201105131259.p4DCxF4C021152-at-ns.microscopy.com} 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4DDM5G5001569 ==============================End of - Headers==============================
I'd like to bring to your attention a problem with hotel booking for M&M 2011 through web. It looks like the web page does not work with new Internet Explorer 9 and with Fox. It shows no vacancies for users with mentioned browsers (I have checked on two computers), but for Explorer 8 it gives rooms in four hotels. Regards,
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: greggps-at-umich.edu [mailto:greggps-at-umich.edu] } Sent: Friday, May 20, 2011 1:03 PM } To: Dusevich, Vladimir } Subject: [Microscopy] RE: RE: Microscope service strategies-- Third- } party } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Chris, } You should also consider that the company may advertise 25% savings, } but on advanced equipment, they will probably back off to 10-15%, since } one unreliable instrument can suck up their pool of money faster than } their profit margin can afford. } } If can you show me an equipment service underwriter's quote for 25% } less than list for a well-equipped confocal or electron microscope, } you'll definitely have my interest. Of course this depends on the size } of the company's pool of contracts. } } Regards, } ~Gregg } } Gregg Sobocinski } Microscope Imaging Specialist } University of Michigan } Molecular, Cellular, and Developmental Biology } Ann Arbor, Michigan } USA } } } } -----Original Message----- } X-from: Christopher.Gilpin-at-utsouthwestern.edu } [mailto:Christopher.Gilpin-at-utsouthwestern.edu] } Sent: Monday, May 16, 2011 3:24 PM } To: Sobocinski, Gregg } Subject: [Microscopy] FW: RE: [spam]* Microscope service strategies } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } } } All, } There is a new option that is floating around the University of Texas } system. An independent company has signed a deal with UT system to save } everyone 25% on their service contracts (not just EMs - a whole range } of } equipment, as long as it is a parts and labor contract). They get you } to } move to an "on demand" arrangement with your existing service provider. } They } pay all the bills and take the chance that you will spend less than a } full } price contract. Of course there is the issue with how service providers } prioritize contract versus on-demand customers. This company will } freeze the } cost for 4 years and as part of the arrangement with UT system (with } qualifying contracts and equipment) will not decline coverage. } } I am tempted but don't like the idea of having less priority when I } make a } service call. } } Regards } } Chris } } } Christopher J Gilpin Ph.D. } Assistant Professor } Director, Molecular and Cellular Imaging Facility } K1.A04 } UT Southwestern Medical Center at Dallas } 5323 Harry Hines Boulevard } Dallas, TX 75390-9039 } Phone +1 214 648 2827 } } -----Original Message----- } X-from: raharris-at-ucdavis.edu [mailto:raharris-at-ucdavis.edu] } Sent: Monday, May 16, 2011 12:38 PM } To: Christopher Gilpin } Subject: [Microscopy] RE: [spam]* Microscope service strategies } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } I have been following this thread intermittently so I do not know if } what I } am suggesting has been mentioned. Also, it is a little off topic so } please } forgive me; I am not trying to high-jack the thread but just to insert } a } comment. In my experience, if the administration of your school or } university is looking for a way to indirectly close down a program, } this is } one of the strategies they will use. I have seen it happen many times } at } the university where I have worked since 1979. } } Good luck, } } Rick A. Harris } } } } } } } ______________________________________ } X-from: nizets2-at-yahoo.com [nizets2-at-yahoo.com] } Sent: Monday, May 16, 2011 1:43 AM } To: Rick A Harris } Subject: [Microscopy] [spam]* Microscope service strategies } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Basically what you are saying is that people with more money are } happier. } This is quite an evidence, isn't it? } I don't think people leave a service contract when they don't have to. } } Regards, } } Stephane } } } } ----- Original Message ---- } X-from: "kenconverse-at-qualityimages.biz" {kenconverse-at-qualityimages.biz} } To: nizets2-at-yahoo.com } Sent: Fri, May 13, 2011 3:25:39 PM } Subject: [Microscopy] RE: [spam]* Microscope service strategies } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Valery, } Very well stated. The only thing I might add is that the customer with } a } service contract is guaranteed certain things, such as preventive } maintenance, operation to specification, perhaps even guaranteed } maximum } down-time. The one-off customer has none of this. The service } organization } is contractually bound to the contract customer. Period. Yes, the one } off } cash is nice, but the regular customers are far more valuable in the } long } run. They often are happier customer, also. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } Sent: Friday, May 13, 2011 8:59 AM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] [spam]* Microscope service strategies } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Dave's message below compelled me to explain a difference between } service } contract and one-off service call from the vendor's perspective. } } For most of FIB/SEM equipment service providers, both small and large, } equipment service is a business activity; it must be profitable or } could not } continue. Delivering service and making it possible also costs } money: most of users do not realize how enormous are the aggregated } expenses } of labor, travel, sourcing parts, re-designing or substituting obsolete } components, warehousing, bean counting, etc... } } When you buy a service contract from the certain provider, it not just } gives } you reliable support with predictable response time, but also gives } that } service organization reliable stream of revenue and predictable work } load. } Stability allows to plan and structure business, retain and maintain } necessary resources, source and stock parts, etc. } } If customer goes off service contract then situation becomes completely } unpredictable: he/she may call in for service, but also may do self- } service, } hire internal service person, or do whatever else. In such situation } managers of the service organization have no other choice but to } prepare for } the worse and assume that there will be no service calls from this } customer. } To insure survival of the organization, cost-saving measures follow } immediately: engineers are laid off, facilities are closed or } downsized, } parts are not procured and not stored, and so forth. These measures are } inevitable - otherwise service organization simply could not continue } to } exist. Reduced resources will usually be matched to the needs of } contract-paying customers, with little to no spare capacity. } } When odd service call comes from the customer without the service } contract, } it is treated as such - one-off, single-case business, which may never } return. Yes, it is nice to get some extra revenue, but (already } reduced) } resources of engineering time and parts will be given to this call only } if } and when all the needs of service-contract customers are taken care of, } and } not a split second earlier. } } The bottom line is that service organization simply can't provide more } service then what it paid for, and overall level of service users } receive } will always degrade in one way or another, as the service revenue } decreases. } } Cheers :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 5/13/2011 5:59 AM, David.Patton-at-uwe.ac.uk wrote: } } } ----------------------------------------------------------------------- } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } ----- } } } } We went off contract last year and had to wait over 4 months for a } service. The engineer said that if it had been a breakdown the delay } would } have been the same. } } } } Dave } } } } -----Original Message----- } } X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] } } Sent: 12 May 2011 17:50 } } To: David Patton } } Subject: [spam]* [Microscopy] Microscope service strategies } } } } } } } } } } } ----------------------------------------------------------------------- } ----- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ----------------------------------------------------------------------- } ----- } } } } Friends: } } } } We are caught on the horns of a dilemma. For nearly forty years, } Delta } College has trained EM technicians for positions in academia and } industry. } For most of those forty years our instruments, now numbering 3 TEM's, } 4, } SEM's, an AFM and an FIB, have been covered by service contracts. Our } instruments are used all day, every day for student training. } } } } As you may have heard, funding for California schools is getting so } } tight } that the bean counters are turning to drastic measures to make ends } meet. } Among pressures put on us is a requirement that we enroll at least 20 } students/class, nearly impossible in our advanced, hand-on classes. In } addition, the administration is now reluctant to authorize service } contracts } for our instruments. } } } } Many or our microscopes are older and require more repairs due to } } their } constant use as training instruments. We have received good service } from our } scope vendor, but it is expensive, mostly because we have so many } instruments. The admin. people only look at the bottom line and when } they } see the big number, they go ballistic. } } } } } We are afraid that if we drop our current service contracts that the } manufacturer will not pick them up if we want to come back after a year } off } their service. We are afraid that if we go to pay as you go with the } manufacturer, we will be low in their priority list, something not good } with } so many students depending on microscope time to finish projects. We } are } afraid that if we can find a cheaper, independent service provider, } parts } will be hard to get and the price of the parts would be in addition to } the } cost of the service agreement, whereas parts are included in our } current } contracts. } } } } This is getting long. Bottom line is we are asking for any advice or } suggestions about how to approach this. I have heard all the horror } stories } about 3rd party insurance plans, etc. Are those stories true? Is it } true } that vendors move you to the bottom of the list without a contract? } What is } your experience if ever faced by this kind of situation? What kind of } strategy has worked, or not? } } } } Hoping for a miracle, } } } } Jon } } } } Jonathan Krupp } } Delta College } } 5151Pacific Ave. } } Stockton, CA 95207 } } 209-954-5284 } } jkrupp-at-deltacollege.edu } } } } } } } } } } } } ==============================Original } Headers============================== } } 13, 34 -- From jkrupp-at-deltacollege.edu Thu May 12 11:47:26 2011 13, } 34 } } -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu } [207.62.178.150]) } } 13, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
On our Zeiss instrument (just } 1 year old) during pump down, the instrument gets to ~9 Pa and progress seems to stop. Under "Vac status" it says "Waiting Penning." There were no error messages when initializing the software, and the green light on the Penning gauge is on. I am no expert, but I suspect that the vacuum system has pumped down as it should but the system can't read any signal from the Penning gauge and it won't allow the gun to fire. I have turned off the tool and restarted it several times, but always with the same problem.
This was the first time the tool has been started since the terrible weather we had in Alabama on April 27th and we lost power for several hours (it was on Standby when the power went out). Again, there appears to be no damage and there are no error messages.
Any suggestions on what the problem may be would be greatly appreciated.
Thank you,
Mark Puckett
T. Markham Puckett, Ph.D. Associate Professor of Geology Department of Physics and Earth Science P.O. Box 5130 University of North Alabama Florence, Alabama 35632-0001 “Why, sometimes I’ve believed as many as six impossible things before breakfast.” The White Queen to Alice, Through the Looking-Glass
==============================Original Headers============================== 6, 18 -- From piper1960-at-hotmail.com Fri May 20 15:17:53 2011 6, 18 -- Received: from col0-omc3-s3.col0.hotmail.com (col0-omc3-s3.col0.hotmail.com [65.55.34.141]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4KKHqGc008612 6, 18 -- for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 15:17:53 -0500 6, 18 -- Received: from COL117-W13 ([65.55.34.137]) by col0-omc3-s3.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 6, 18 -- Fri, 20 May 2011 13:16:24 -0700 6, 18 -- Message-ID: {COL117-W13F7EE103E35FC7DA0BCE2BF710-at-phx.gbl} 6, 18 -- X-Originating-IP: [204.29.102.55] 6, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} 6, 18 -- To: {microscopy-at-microscopy.com} 6, 18 -- Subject: 6, 18 -- Date: Fri, 20 May 2011 15:16:24 -0500 6, 18 -- Importance: Normal 6, 18 -- Content-Type: text/plain; charset="Windows-1252" 6, 18 -- MIME-Version: 1.0 6, 18 -- X-OriginalArrivalTime: 20 May 2011 20:16:24.0414 (UTC) FILETIME=[CAB9C7E0:01CC172A] 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4KKHqGc008612 ==============================End of - Headers==============================
Hi Jonathan, if you are able to send me some photos of your CR21 system and the controller, I might be able to help you. I have the service manual etc. Depends on what you like to section you should be able to make decent glass knifes or have a cryo diamond knife :-) I am doing RMC service for Germany. If anything other fails I can get you in touch with RMC at Tucson.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 20.05.11 22:10, schrieb jkrupp-at-deltacollege.edu: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } I have a student who would like to section some polymer samples. We figure we will need to cool them to make this work. } } Our cryo resources a limited, an RMC MT-7 and some parts for the CR-21 attachment, but it is not complete. } } Any ideas for how to proceed or jury-rig a home made system? } } Thanks } } Jon } } Jonathan Krupp } Delta College } 5151Pacific Ave. } Stockton, CA 95207 } 209-954-5284 } jkrupp-at-deltacollege.edu } } } } } } ==============================Original Headers============================== } 11, 34 -- From jkrupp-at-deltacollege.edu Fri May 20 15:08:33 2011 } 11, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) } 11, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4KK8W7g024936 } 11, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 15:08:32 -0500 } 11, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) } 11, 34 -- by localhost (Email Security Appliance) with SMTP id 4AB2895CD07_DD6CA40B } 11, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 20:08:32 +0000 (GMT) } 11, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) } 11, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 31F3F95CD09_DD6CA40F } 11, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 20:08:32 +0000 (GMT) } 11, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.178]) by } 11, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with } 11, 34 -- ESMTP id LLIFY700.A58 for {Microscopy-at-microscopy.com} ; Fri, 20 } 11, 34 -- May 2011 13:08:31 -0700 } 11, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) } 11, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id F3E01827A359 } 11, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 13:08:31 -0700 (PDT) } 11, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) } 11, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) } 11, 34 -- with ESMTP id Y4J+cugZ1QcE for {Microscopy-at-microscopy.com} ; } 11, 34 -- Fri, 20 May 2011 13:08:27 -0700 (PDT) } 11, 34 -- Received: from [172.20.3.86] (unknown [172.20.3.86]) } 11, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id EAA08827A326 } 11, 34 -- for {Microscopy-at-microscopy.com} ; Fri, 20 May 2011 13:08:27 -0700 (PDT) } 11, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} } 11, 34 -- Content-Type: text/plain; charset=us-ascii } 11, 34 -- Subject: Polymer cryo sectioning } 11, 34 -- Date: Fri, 20 May 2011 13:08:27 -0700 } 11, 34 -- Message-Id: {69FC48C8-9BDD-4D76-B0F1-F15F60613DB1-at-deltacollege.edu} } 11, 34 -- To: Microscopy-at-microscopy.com } 11, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) } 11, 34 -- X-Mailer: Apple Mail (2.1084) } 11, 34 -- Content-Transfer-Encoding: 8bit } 11, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4KK8W7g024936 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 22 -- From stefan.diller-at-t-online.de Fri May 20 15:21:10 2011 8, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 8, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4KKL9Nl015796 8, 22 -- for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 15:21:10 -0500 8, 22 -- Received: from fwd17.aul.t-online.de (fwd17.aul.t-online.de ) 8, 22 -- by mailout08.t-online.de with smtp 8, 22 -- id 1QNWC4-0003RW-Sb; Fri, 20 May 2011 22:21:08 +0200 8, 22 -- Received: from mac-pro.speedport.ip (Vrc5I8ZfQhyfc0CwLWQKGbu4EnFa0obGbXNPyQH2QoKza0+-jsPg3WnfBWYDjJOwZ1-at-[93.222.127.25]) by fwd17.t-online.de 8, 22 -- with esmtp id 1QNWC2-17IsE40; Fri, 20 May 2011 22:21:06 +0200 8, 22 -- Message-ID: {4DD6CD32.70301-at-t-online.de} 8, 22 -- Date: Fri, 20 May 2011 22:21:06 +0200 8, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 8, 22 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 8, 22 -- MIME-Version: 1.0 8, 22 -- To: microscopy-at-microscopy.com 8, 22 -- Subject: Re: [Microscopy] Polymer cryo sectioning 8, 22 -- References: {201105202010.p4KKAwHn031017-at-ns.microscopy.com} 8, 22 -- In-Reply-To: {201105202010.p4KKAwHn031017-at-ns.microscopy.com} 8, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 22 -- Content-Transfer-Encoding: 7bit 8, 22 -- X-ID: Vrc5I8ZfQhyfc0CwLWQKGbu4EnFa0obGbXNPyQH2QoKza0+-jsPg3WnfBWYDjJOwZ1 8, 22 -- X-TOI-MSGID: 0c5cc5b3-5c74-40f5-aa6f-628032fc5c61 ==============================End of - Headers==============================
On our Zeiss instrument (just over 1 year old) during pump down, the instrument gets to 9 Pa and progress seems to stop. Under "Vac status" it says "Waiting Penning." There were no error messages when initializing the software, and the green light on the Penning gauge is on. I am no expert, but I suspect that the vacuum system has pumped down as it should but the system can't read any signal from the Penning gauge and it won't allow the gun to fire.
This was the first time the tool has been started since the terrible weather we had in Alabama on April 27th and we lost power for several hours (it was on Standby when the power went out). I have turned off the tool and restarted it several times, but always with the same problem. Again, there appears to be no damage and there are no error messages.
Any suggestions on what the problem may be would be greatly appreciated.
Thank you,
Mark Puckett
T. Markham Puckett, Ph.D.
Associate Professor of Geology
Department of Physics and Earth Science
P.O. Box 5130
University of North Alabama
Florence, Alabama 35632-0001
“Why, sometimes I’ve believed as many as six impossible things before breakfast.” The White Queen to Alice, Through the Looking-Glass
==============================Original Headers============================== 24, 18 -- From piper1960-at-hotmail.com Fri May 20 15:27:19 2011 24, 18 -- Received: from col0-omc3-s2.col0.hotmail.com (col0-omc3-s2.col0.hotmail.com [65.55.34.140]) 24, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4KKRJgx004869 24, 18 -- for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 15:27:19 -0500 24, 18 -- Received: from COL117-W42 ([65.55.34.137]) by col0-omc3-s2.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 24, 18 -- Fri, 20 May 2011 13:27:18 -0700 24, 18 -- Message-ID: {COL117-W4241347A690ADF9FE34529BF710-at-phx.gbl} 24, 18 -- X-Originating-IP: [204.29.102.55] 24, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} 24, 18 -- To: {microscopy-at-microscopy.com} 24, 18 -- Subject: Penning gauge on Zeiss EVO MA10 24, 18 -- Date: Fri, 20 May 2011 15:27:18 -0500 24, 18 -- Importance: Normal 24, 18 -- Content-Type: text/plain; charset="Windows-1252" 24, 18 -- MIME-Version: 1.0 24, 18 -- X-OriginalArrivalTime: 20 May 2011 20:27:18.0777 (UTC) FILETIME=[50C1A690:01CC172C] 24, 18 -- Content-Transfer-Encoding: 8bit 24, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4KKRJgx004869 ==============================End of - Headers==============================
With our 912 the IGPs take a bit of time to pump down and during this the Penning gauge message is displayed. Due to your long hiatus from active pumping I bet you will need to have the instrument pump down over the weekend before it reaches hi vac. Hope you survived the storms OK!
Howard
On May 20, 2011, at 3:28 PM, {piper1960-at-hotmail.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Hi All, } } Sorry if you received the message twice. } } On our Zeiss instrument (just over 1 year old) during pump down, the instrument gets to 9 Pa and progress seems to stop. Under "Vac status" it says "Waiting Penning." There were no error messages when initializing the software, and the green light on the Penning gauge is on. I am no expert, but I suspect that the vacuum system has pumped down as it should but the system can't read any signal from the Penning gauge and it won't allow the gun to fire. } } This was the first time the tool has been started since the terrible weather we had in Alabama on April 27th and we lost power for several hours (it was on Standby when the power went out). I have turned off the tool and restarted it several times, but always with the same problem. Again, there appears to be no damage and there are no error messages. } } Any suggestions on what the problem may be would be greatly appreciated. } } Thank you, } } Mark Puckett } } } } T. Markham Puckett, Ph.D. } } } Associate Professor of Geology } } } Department of Physics and Earth Science } } } P.O. Box 5130 } } } University of North Alabama } } } Florence, Alabama 35632-0001 } } } } } “Why, sometimes I’ve believed as many as six impossible things before breakfast.” The White Queen to Alice, Through the Looking-Glass } } ==============================Original Headers============================== } 24, 18 -- From piper1960-at-hotmail.com Fri May 20 15:27:19 2011 } 24, 18 -- Received: from col0-omc3-s2.col0.hotmail.com (col0-omc3-s2.col0.hotmail.com [65.55.34.140]) } 24, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4KKRJgx004869 } 24, 18 -- for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 15:27:19 -0500 } 24, 18 -- Received: from COL117-W42 ([65.55.34.137]) by col0-omc3-s2.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); } 24, 18 -- Fri, 20 May 2011 13:27:18 -0700 } 24, 18 -- Message-ID: {COL117-W4241347A690ADF9FE34529BF710-at-phx.gbl} } 24, 18 -- X-Originating-IP: [204.29.102.55] } 24, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} } 24, 18 -- To: {microscopy-at-microscopy.com} } 24, 18 -- Subject: Penning gauge on Zeiss EVO MA10 } 24, 18 -- Date: Fri, 20 May 2011 15:27:18 -0500 } 24, 18 -- Importance: Normal } 24, 18 -- Content-Type: text/plain; charset="Windows-1252" } 24, 18 -- MIME-Version: 1.0 } 24, 18 -- X-OriginalArrivalTime: 20 May 2011 20:27:18.0777 (UTC) FILETIME=[50C1A690:01CC172C] } 24, 18 -- Content-Transfer-Encoding: 8bit } 24, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4KKRJgx004869 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 32 -- From RHBerg-at-danforthcenter.org Fri May 20 17:02:32 2011 9, 32 -- Received: from spm1.danforthcenter.org (spm1.danforthcenter.org [65.254.111.26]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4KM2Wog026176 9, 32 -- for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 17:02:32 -0500 9, 32 -- Received: from spm1.danforthcenter.org (127.0.0.1) by spm1.danforthcenter.org (MlfMTA v3.2r9) id hqrifg0171s6 for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 17:02:32 -0500 (envelope-from {RHBerg-at-danforthcenter.org} ) 9, 32 -- Received: from spm1.danforthcenter.org ([10.101.0.44]) 9, 32 -- by spm1.danforthcenter.org (SonicWALL 7.2.4.3927) 9, 32 -- with ESMTP (AIO); Fri, 20 May 2011 17:02:32 -0500 9, 32 -- Received: from mail2.ddpsc.org ([::1]) by mail2.ddpsc.org ([::1]) with mapi; 9, 32 -- Fri, 20 May 2011 17:02:32 -0500 9, 32 -- From: "Berg, R. Howard" {RHBerg-at-danforthcenter.org} 9, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 32 -- Date: Fri, 20 May 2011 17:02:31 -0500 9, 32 -- Subject: Re: [Junk released by Policy action] [Microscopy] Penning gauge on 9, 32 -- Zeiss EVO MA10 9, 32 -- Thread-Topic: [Junk released by Policy action] [Microscopy] Penning gauge on 9, 32 -- Zeiss EVO MA10 9, 32 -- Thread-Index: AcwXOZ28x4xS9JLiQl2ydn0M7XUUyw== 9, 32 -- Message-ID: {09951FAA-33A3-454F-895D-D9FA2ADD74DA-at-danforthcenter.org} 9, 32 -- References: {201105202028.p4KKSJZU008721-at-ns.microscopy.com} 9, 32 -- In-Reply-To: {201105202028.p4KKSJZU008721-at-ns.microscopy.com} 9, 32 -- Accept-Language: en-US 9, 32 -- Content-Language: en-US 9, 32 -- X-MS-Has-Attach: 9, 32 -- X-MS-TNEF-Correlator: 9, 32 -- acceptlanguage: en-US 9, 32 -- Content-Type: text/plain; charset="Windows-1252" 9, 32 -- MIME-Version: 1.0 9, 32 -- X-Mlf-Version: 7.2.4.3927 9, 32 -- X-Mlf-UniqueId: o201105202202320019633 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4KM2Wog026176 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dietz-at-anl.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: dietz-at-anl.gov Name: Nancy
Organization: ANL
Title-Subject: [Filtered] AC fields
Message: Erase this text and type your question here Has anyone effectively removed AC fields by shielding the column/specimen chamber by using GIRON or Magnetic Shielding Foil? The magnetic field in question is around 1-3 mG. The alternative is an expensive Helmholtz cage. Thank you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both D.L.Bayliss-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: D.L.Bayliss-at-lboro.ac.uk Name: Danny Bayliss
Organization: Loughborough University
Title-Subject: [Filtered] TEM: Bacterial sample preparation and staining
Message: Hi All, I wanted to prepare some bacteria in solution for TEM imaging. Unfortunately at my university the people responsible for the TEM havenÂ’t had experience in preparing biological samples so I am going it alone. My question is regarding the best protocol for what I want to image. I am inactivating bacteria (Listeria, gram positive) and trying to understand the mechanism, so far SEM imaging reveals no visual morphological damage but flow cytometry shows PI staining meaning there is membrane damage, I would like to use TEM to visualise this membrane damage. I am looking for the best protocol that will help preserve and stain the membrane. I understand fixing is probably best with Gluteraldehyde then post fixing with Osmium Tetroxide (OSO4) but what is the best buffer to use with these fixing agents? I have read that CaCl2 can help to retain lipids that may be lost during fixing but I wanted some guidance from people who have experience with preparing bacterial samples for the best conditions.
For my second question I apologise in advance if this sounds like a stupid question but I have also read protocols that suspend fixed bacteria in agrose then centrifuge and allow the pellet to solidify. Is this just to make the sample easier to manipulate for the subsequent dehydration and embedding steps?
My last question is regarding staining, what is the best stain or stains typically used for bacterial membranes to give a good contrast and when is it best to stain? Do you commonly stain during fixing, before dehydration or after embedding?
I look forward to hearing any advice you have to help me.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: z.zhou-at-lboro.ac.uk Name: Zhaoxia Zhou
Organization: Loughborough University UK
Title-Subject: [Filtered] TEM dislocation density measurement
Message: Greetings to all,
A student studying Ni-based super-alloys asked how to measure dislocation densities using a 2000FX TEM. I understand that grains can be tilted to certain orientations to make dislocations visible/invisible, eventually the burgers vector of the dislocations can be worked out. Does that mean at no orientation all the dislocations are visible? How do we then quantify the density of dislocations? Any advice, references, books, manuals are highly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bloos-at-sun.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bloos-at-sun.ac.za Name: Ben Loos
Organization: Stellenbosch University
Title-Subject: [Filtered] fluorescent probes and their hydrophobic/hydrophilic character
Message: Dear colleagues,
I trust you are well? May be some one of you can advice? What type of fluorescent probes are of high hydrophobic and which ones of high hydrophilic character? I get increasing users from the polymer science field, where this information will be helpful.
could anyone advice? a nice and narrow spectral ex/em pattern will of course be useful to avoid bleedthrough. thanks alot and best wishes Ben
Dr Ben Loos Cell Imaging Unit -Fluorescence Live Cell Imaging & Flow Cytometry- Central Analytical Facility-CAF Stellenbosch University - South Africa Department of Physiological Sciences Mike de Vries Building Office 2022 7600 Stellenbosch Tel: +27 21 808 9196 Fax:+27 21 808 3145 email: bloos-at-sun.ac.za website: http://academic.sun.ac.za/saf
piper1960-at-hotmail.com wrote: } On our Zeiss instrument (just over 1 year old) during pump down, the } instrument gets to 9 Pa and progress seems to stop. Under "Vac status" } it says "Waiting Penning." There were no error messages when } initializing the software, and the green light on the Penning gauge is } on. I am no expert, but I suspect that the vacuum system has pumped } down as it should but the system can't read any signal from the } Penning gauge and it won't allow the gun to fire.
Hello Mark, this is the question for Zeiss service people, isn't it? Please continue reading just in a case you have confidence in a friendly competitor's advice :-). As the pressure readout stops at 9 Pa, I do not think longer pumping would help - such pressure is too high. Try to connect your external gauge somewhere on the chamber or nearby. In a case you measure lower pressure on your gauge, there is a problem related to the ignition of the microscope penning gauge - just clean it or replace it. In a case you will measure the same pressure at the external gauge, there is a leak somewhere - in that case, you would probably observe overheating of the turbomolecular pump after an extended pumping period. However do not count these advices as absolutely correct and believe more Zeiss advices :-). Good luck, Tomas
I recommend you to have a look at the nice "Handbook" from Molecular probes. Most fluorescent probes for cell biology are hydrophilic and the choice is really big. For hydrophobic/lipophilic probes you may look at "Probes for lipids and membranes":
Also please consider other important parameters like the wavelengthes (for optimal signal they must fit your lasers) and quantum yield. I have no direct interest with molecular probes. I am just a regular customer of their products for 15 years and always found them of very high quality (and price, too).
Best regards,
Stephane
----- Original Message ---- X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: nizets2-at-yahoo.com Sent: Sat, May 21, 2011 5:38:33 AM
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bloos-at-sun.ac.za as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bloos-at-sun.ac.za Name: Ben Loos
Organization: Stellenbosch University
Title-Subject: [Filtered] fluorescent probes and their hydrophobic/hydrophilic character
Message: Dear colleagues,
I trust you are well? May be some one of you can advice? What type of fluorescent probes are of high hydrophobic and which ones of high hydrophilic character? I get increasing users from the polymer science field, where this information will be helpful.
could anyone advice? a nice and narrow spectral ex/em pattern will of course be useful to avoid bleedthrough. thanks alot and best wishes Ben
Dr Ben Loos Cell Imaging Unit -Fluorescence Live Cell Imaging & Flow Cytometry- Central Analytical Facility-CAF Stellenbosch University - South Africa Department of Physiological Sciences Mike de Vries Building Office 2022 7600 Stellenbosch Tel: +27 21 808 9196 Fax:+27 21 808 3145 email: bloos-at-sun.ac.za website: http://academic.sun.ac.za/saf
I'm using Vincent Hou's Image recorder script (posted on the University of Graz DM website) to capture ronchigrams and CBED patterns on a CCD and also ADF images via a Digiscan II. Works well!! Now what I want to do is scan the probe between image acquisitions. I don't want a stationary probe unless I'm recording the rochigram, CBED patterns. With the ADF signal I can synch the image recorder with the ADF refresh rate. Does anyone have a script that controls the probe via the Digiscan or knows of a method to achieve this.
I've been told the way to do it is to define an ROI 1 pixel in size and scan this ROI. I can find examples to define an ROI on the University of Graz website but nothing on actually controlling the beam or the ROI, ie the commands to use or a script that demonstrates this.
Any hints, help gratefully appreciated.
Cheers George
==============================Original Headers============================== 5, 28 -- From george.theodossiou-at-st-annes.ox.ac.uk Mon May 23 05:35:05 2011 5, 28 -- Received: from relay4.mail.ox.ac.uk (relay4.mail.ox.ac.uk [129.67.1.163]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4NAZ4YX024994 5, 28 -- for {Microscopy-at-Microscopy.com} ; Mon, 23 May 2011 05:35:05 -0500 5, 28 -- Received: from smtp2.nexus.ox.ac.uk ([163.1.154.136] helo=exht02.ad.oak.ox.ac.uk) 5, 28 -- by relay4.mail.ox.ac.uk with esmtp (Exim 4.75) 5, 28 -- (envelope-from {george.theodossiou-at-st-annes.ox.ac.uk} ) 5, 28 -- id 1QOSTY-0001cQ-D4 5, 28 -- for Microscopy-at-Microscopy.com; Mon, 23 May 2011 11:35:04 +0100 5, 28 -- Received: from EXMBX01.ad.oak.ox.ac.uk ([169.254.2.200]) by 5, 28 -- exht02.ad.oak.ox.ac.uk ([163.1.154.53]) with mapi; Mon, 23 May 2011 11:35:03 5, 28 -- +0100 5, 28 -- From: George Theodossiou {george.theodossiou-at-st-annes.ox.ac.uk} 5, 28 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com} 5, 28 -- Date: Mon, 23 May 2011 11:35:03 +0100 5, 28 -- Subject: Digital Micrograph Scripting - Probe/Beam Control 5, 28 -- Thread-Topic: Digital Micrograph Scripting - Probe/Beam Control 5, 28 -- Thread-Index: AQHMGTUTwykFnFrTpUic3T+K9OY3FA== 5, 28 -- Message-ID: {CFADFFD51D0744438FC8E1A5A990E4DF89288A9D4E-at-EXMBX01.ad.oak.ox.ac.uk} 5, 28 -- Accept-Language: en-US, en-GB 5, 28 -- Content-Language: en-GB 5, 28 -- X-MS-Has-Attach: 5, 28 -- X-MS-TNEF-Correlator: 5, 28 -- acceptlanguage: en-US, en-GB 5, 28 -- Content-Type: text/plain; charset="us-ascii" 5, 28 -- MIME-Version: 1.0 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4NAZ4YX024994 ==============================End of - Headers==============================
Hello Listers, I am using an RMC MT-7000 and recently I have replaced the belt that connects the ~"Autocut" motor to the hand-wheel. As the hand-wheel is rotated the specimen arm moves up and down but the specimen arm does not advance. It can be clearly seen that the stepper motor is not rotating as I manually move the hand-wheel, or use the Autocut feature. I have confirmed that the stepper motor is functioning(hitting the "Return" button or the "Step Advance" the motor rotates in the correct fashion). Prior to replacing the belt the microtome the specimen arm was advancing just fine. What have I mucked up? Any help/advice will be greatly appreciated!
-Joe
-- Joseph Heintz Lab/BBPIC Manager University of Wisconsin-Madison Room 1048, Animal Sciences Building 1675 Observatory Dr. Madison, WI 53706 608.263.4162 BBPIC Facility Website
==============================Original Headers============================== 3, 33 -- From josephheintz-at-gmail.com Mon May 23 07:32:42 2011 3, 33 -- Received: from mail-ew0-f41.google.com (mail-ew0-f41.google.com [209.85.215.41]) 3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4NCWg9Y010330 3, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 23 May 2011 07:32:42 -0500 3, 33 -- Received: by ewy9 with SMTP id 9so2113111ewy.0 3, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 23 May 2011 05:32:41 -0700 (PDT) 3, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 33 -- d=gmail.com; s=gamma; 3, 33 -- h=domainkey-signature:mime-version:sender:from:date 3, 33 -- :x-google-sender-auth:message-id:subject:to:content-type; 3, 33 -- bh=onkx7pp1pDS6liTPIJcHFYPc5+hi5YsLnect60bY3M0=; 3, 33 -- b=V+gWTJTGvkcRaYLzwFa4DCfFsH0k+iBRupK9GLD9+W6SjmetfPx35VKSOlt04gs4a6 3, 33 -- YXpMO7wIL7Sqejs5+B8fSbO5ysxm7bNbpyK4cz4M+y13WpkjWM3jGKAfProG3Dq7Lkcy 3, 33 -- GGSU066nhyuO0Qru+jbFCM8wYsWDoHW2hutUg= 3, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 33 -- d=gmail.com; s=gamma; 3, 33 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 3, 33 -- :subject:to:content-type; 3, 33 -- b=g6eyfLe0wJXoYB6HrqDUzALK0aCcRUbwlBO/IHV/qGNjOFuvqEySq+++TLlKW6QqRB 3, 33 -- JNjtQxxURX4Yb+EI4m6rXflWYc1LKPVE2Kxt/pSCXfUAUVfwszylEajcu+u5l/NnDCpt 3, 33 -- bHShKIsP35hTtqSSA3KmpZsaahnunOaGtsIQA= 3, 33 -- Received: by 10.14.15.15 with SMTP id e15mr754059eee.231.1306153961268; Mon, 3, 33 -- 23 May 2011 05:32:41 -0700 (PDT) 3, 33 -- MIME-Version: 1.0 3, 33 -- Sender: josephheintz-at-gmail.com 3, 33 -- Received: by 10.14.97.10 with HTTP; Mon, 23 May 2011 05:32:21 -0700 (PDT) 3, 33 -- From: Joseph Heintz {jheintz-at-wisc.edu} 3, 33 -- Date: Mon, 23 May 2011 07:32:21 -0500 3, 33 -- X-Google-Sender-Auth: Ns6BrMYCYVgEJs9Aous73vVrQ2c 3, 33 -- Message-ID: {BANLkTi=yAGdFPOJWtcvF0+ADycRtzNxH7w-at-mail.gmail.com} 3, 33 -- Subject: Microtome Advancement Issue 3, 33 -- To: Microscopy-at-microscopy.com 3, 33 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both meetingmanager-at-microscopy.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: meetingmanager-at-microscopy.org Name: Nicole Guy
Organization: M&M 2011 Meeting Manager
Title-Subject: [Filtered] Online Hotel Reservations site for M&M meeting
Message: For those of you who are using Internet Explorer 9, please note that the hotel reservations website is incompatible with the newest version of IE. If you are using IE 9, it will appear as though there are no hotels available in Nashville to book for the meeting. There are plenty of hotel rooms available at all our conference hotels.
Internet Explorer 7 and 8, Chrome, Firefox and Safari all work just fine. Please use an alternate browser if you can; if not, please call our housing bureau call center at TOLL FREE: 1-877-259-4716 (U.S. & Canada Only; 9:00 am - 5pm CT Monday-Friday.
We do apologize for the inconvenience. If you need a PDF form to fax or mail in, you can obtain that by going to this URL: http://www.microscopy.org/MandM/2011/housingform.pdf
Contrast from dislocations can only arise if the crystal is oriented in such a way that the strain field scatters the Bloch waves set up by diffraction. Although dislocation contrast in aluminium was discovered by Peter Hirsch and co-workers back in 1956, Archie Howie and Michael Whelan explained the dynamical diffraction contrast of imperfect crystals with phenomenal results. Much of this is contained within what is considered to the 'The Bible' of electron microscopy: "Electron Microscopy of Thin Crystals" by Hirsch, Howie, Whelan, Pashley & Nicholson. Several different editions of the book exist and it is still highly recommended as a text book. Mike Loretto's book "Defect Analysis in Electron Microscopy" is a shorter, and in some ways more accessible book to consult, with helpful diffraction patterns and Kikuchi line maps in the Appendices that assist navigation to the required diffraction conditions. More recent books include Barry Carter & David Williams "Transmission Electron Microscopy" which is aided by a selection of some the the most beautiful pictures of defects to have appeared in the past 55 years. Some of the best images seared into my mind date back from the 1970's!
All of these books (and many more) will tell you that successful dislocation analysis requires setting up well known diffraction conditions for each crystal grain. Therefore, no single orientation can give you all the information you want.
Identifying dislocation Burgers vectors requires multiple images of the same set of dislocations under diffraction conditions to see which ones disappear, i.e. dislocations are identified by a process of elimination. However, it is reasonably straightforward to find a set of reflections in which all dislocations will eventually appear. Therefore, the dislocation density can established only if all crystal grains have each diffracted strongly with several different reflections. It is painstaking to do, but is worth the effort.
With a microscope like the JEOL 2000FX, it should be relatively easy and fun to do- the JEOL 2000FX still remains one of my favourite microscopes to do this sort of work, because the condenser optics gives a very bright beam, even for a thermionic emitter. Dislocation 'hunting' on the 2000FX is probably one of the more enjoyable things one can do on a TEM. Happy hunting!
Jon
} Email: z.zhou-at-lboro.ac.uk Name: Zhaoxia Zhou } Organization: Loughborough University UK } Title-Subject: [Filtered] TEM dislocation density measurement } } Message: Greetings to all, } } A student studying Ni-based super-alloys asked how to measure } dislocation densities using a 2000FX TEM. } I understand that grains can be tilted to certain orientations to make } dislocations visible/invisible, eventually the burgers vector of the } dislocations can be worked out. Does that mean at no orientation all the } dislocations are visible? How do we then quantify the density of } dislocations? } Any advice, references, books, manuals are highly appreciated. } } Zhou
==============================Original Headers============================== 8, 28 -- From jsb43-at-hermes.cam.ac.uk Mon May 23 10:39:10 2011 8, 28 -- Received: from ppsw-52.csi.cam.ac.uk (ppsw-52.csi.cam.ac.uk [131.111.8.152]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4NFd9bM013755 8, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 23 May 2011 10:39:10 -0500 8, 28 -- X-Cam-AntiVirus: no malware found 8, 28 -- X-Cam-SpamDetails: not scanned 8, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 8, 28 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:58781) 8, 28 -- by ppsw-52.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.159]:25) 8, 28 -- with esmtpa (EXTERNAL:jsb43) id 1QOXDp-0001Hl-EU (Exim 4.72) 8, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Mon, 23 May 2011 16:39:09 +0100 8, 28 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk) 8, 28 -- with local (PRAYER:jsb43) id 1QOXDp-00086P-FV (Exim 4.67) 8, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Mon, 23 May 2011 16:39:09 +0100 8, 28 -- Received: from [128.232.229.180] by webmail.hermes.cam.ac.uk 8, 28 -- with HTTP (Prayer-1.3.3); 23 May 2011 16:39:09 +0100 8, 28 -- Date: 23 May 2011 16:39:09 +0100 8, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 8, 28 -- To: z.zhou-at-lboro.ac.uk 8, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 8, 28 -- Subject: Re: TEM dislocation density measurement 8, 28 -- Message-ID: {Prayer.1.3.3.1105231639090.10109-at-hermes-2.csi.cam.ac.uk} 8, 28 -- In-Reply-To: {201105210338.p4L3cp4M006384-at-ns.microscopy.com} 8, 28 -- References: {201105210338.p4L3cp4M006384-at-ns.microscopy.com} 8, 28 -- X-Mailer: Prayer v1.3.3 8, 28 -- Mime-Version: 1.0 8, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 8, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
Dear Joe, if your encoder on the shaft which drives the up and down movement of the microtome cutting arm is misaligned and not in the mid of the stroke: this will disturb the microcontroller and it won`t send an advance signal.
Check for the alignment of the decoder (left hand side of the shaft...). Be sure that either when the knob on the handwheel is at "3 o`clock" or the cutting arm is at the knife edge, the VISUTRAC should be in the mid of the LED scale.
If not you have to readjust the decoder (hopefully you did not ruin it during changing the drive-belts because it is very fragile and the distance between the encoder disc and the infrared LEDs should be set very precisely... You need a VERY small ALLEN key to do this ;-)
It might also be an electronics problem but I doubt this when you can advance manually... I send more information offline.
Feel free to contact dave-at-boeckeler.com for more help.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 23.05.11 14:36, schrieb jheintz-at-wisc.edu: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Listers, } I am using an RMC MT-7000 and recently I have replaced the belt that } connects the ~"Autocut" motor to the hand-wheel. As the hand-wheel is } rotated the specimen arm moves up and down but the specimen arm does } not advance. It can be clearly seen that the stepper motor is not } rotating as I manually move the hand-wheel, or use the Autocut } feature. I have confirmed that the stepper motor is } functioning(hitting the "Return" button or the "Step Advance" the } motor rotates in the correct fashion). Prior to replacing the belt the } microtome the specimen arm was advancing just fine. What have I mucked } up? Any help/advice will be greatly appreciated! } } -Joe }
==============================Original Headers============================== 10, 22 -- From stefan.diller-at-t-online.de Mon May 23 11:01:52 2011 10, 22 -- Received: from mailout11.t-online.de (mailout11.t-online.de [194.25.134.85]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4NG1quU030131 10, 22 -- for {microscopy-at-microscopy.com} ; Mon, 23 May 2011 11:01:52 -0500 10, 22 -- Received: from fwd13.aul.t-online.de (fwd13.aul.t-online.de ) 10, 22 -- by mailout11.t-online.de with smtp 10, 22 -- id 1QOXZn-0003j5-9I; Mon, 23 May 2011 18:01:51 +0200 10, 22 -- Received: from mac-pro.speedport.ip (TlseWOZSZhrrSAcnJWm8a1x--LrtZpRpG9BJb8ePcaD1IhIHOt1iCD2JjNUQUl3wjj-at-[93.222.116.238]) by fwd13.t-online.de 10, 22 -- with esmtp id 1QOXZh-1zl6i80; Mon, 23 May 2011 18:01:45 +0200 10, 22 -- Message-ID: {4DDA84E9.1010009-at-t-online.de} 10, 22 -- Date: Mon, 23 May 2011 18:01:45 +0200 10, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 10, 22 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 10, 22 -- MIME-Version: 1.0 10, 22 -- To: microscopy-at-microscopy.com 10, 22 -- Subject: Re: [Microscopy] Microtome Advancement Issue 10, 22 -- References: {201105231236.p4NCa43I016425-at-ns.microscopy.com} 10, 22 -- In-Reply-To: {201105231236.p4NCa43I016425-at-ns.microscopy.com} 10, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 22 -- Content-Transfer-Encoding: 7bit 10, 22 -- X-ID: TlseWOZSZhrrSAcnJWm8a1x--LrtZpRpG9BJb8ePcaD1IhIHOt1iCD2JjNUQUl3wjj 10, 22 -- X-TOI-MSGID: b1286d38-5ecb-46f3-8ee0-41cefbb8d5b6 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: michael cammer
Organization: Skirball Institute at NYU Langone Medical College
Title-Subject: [Filtered] seeking fluorescence for Olympus IMT-2
Message: We have a stripped down Olympus IMT-2 that people have been using for screening their cell cultures. Colleagues have requested fluorescence on this microscope.
Does anyone have an illumination pillar and arc lamp for this microscope that they, perhaps, don't need? Also, any extra filter blocks (we have one)? We'd also welcome inexpensive proposals for commerical solutions. Please contact off-line at michael.cammer-at-med.nyu.edu.
Hi - We are giving away a JEOL JXA 733 microprobe. I believe it is working (except for the EDS detector). It is free as long as interested parties arrange to pick it up. Please let me know if you are interested. -Dan
----------------------------------- Dan Ruscitto
Laboratory Manager 1W13 Jonsson-Rowland Science Center Earth & Environmental Sciences Rensselaer Polytechnic Institute 110 8th St. Troy NY 12180 Ph: 518-276-2372 Fax: 518-276-2012
==============================Original Headers============================== 9, 24 -- From ruscid2-at-rpi.edu Mon May 23 17:30:37 2011 9, 24 -- Received: from smtp8.server.rpi.edu (smtp8.server.rpi.edu [128.113.2.228]) 9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4NMUbdl004288 9, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 May 2011 17:30:37 -0500 9, 24 -- Received: from [192.168.0.12] (cpe-24-92-47-165.nycap.res.rr.com [24.92.47.165]) 9, 24 -- (authenticated bits=0) 9, 24 -- by smtp8.server.rpi.edu (8.13.1/8.13.1) with ESMTP id p4NMUZoO020644 9, 24 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=NO) 9, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 23 May 2011 18:30:36 -0400 9, 24 -- From: Dan Ruscitto {ruscid2-at-rpi.edu} 9, 24 -- Content-Type: text/plain; charset=us-ascii 9, 24 -- Subject: Free JEOL JXA 733 microprobe 9, 24 -- Date: Mon, 23 May 2011 18:30:35 -0400 9, 24 -- Message-Id: {ACAA84D1-3AB5-414F-8F26-CB80DEFE2C48-at-rpi.edu} 9, 24 -- To: Microscopy-at-microscopy.com 9, 24 -- Mime-Version: 1.0 (Apple Message framework v1084) 9, 24 -- X-Mailer: Apple Mail (2.1084) 9, 24 -- X-Bayes-Prob: 0.0001 (Score 0) 9, 24 -- X-RPI-SA-Score: 0.00 () [Hold at 12.00] 22490(-25) 9, 24 -- X-CanItPRO-Stream: outgoing 9, 24 -- X-Canit-Stats-ID: Bayes signature not available 9, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.113.2.228 9, 24 -- Content-Transfer-Encoding: 8bit 9, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4NMUbdl004288 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ram-at-cen.dtu.dk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ram-at-cen.dtu.dk Name: Ramona
Organization: DTU Cen
Title-Subject: [Filtered] Sample prep of Pseudomonas Aeruginosa
Message: Dear all, I was wondering if any of you have experience with preparation of Pseudomonas Aeruginosa samples grown on Agar plates for SEM. I am interested in being able to have a bit of the Agar substrate with the bacteria in the SEM. We are able to do LVSEM, ESEM, Peltier, Cryo SEM and even TEM; so whatever protocol, article review and experience will be appreciated :-) Regards, Ramona
Check out "Ultrastructural preservation of biofilms formed by non-typeable Hemophilus influenzae" P. Webster, S. Wu , S. Webster, K.A.Rich and K. McDonald. Biofilms (2004) 1, 165–182, for a great SEM method.
and also
"High-Resolution Visualization of Pseudomonas aeruginosa PAO1 Biofilms by Freeze-Substitution Transmission Electron Microscopy" Ryan C. Hunter* and Terry J. Beveridge, for interesting stuff about Pseudomonas aeruginosa Journal of Bacteriology , Nov. 2005, p. 7619–7630 Vol. 187, No. 22
Let me know if you have trouble getting them and I will send you the PDF's.
Good luck
Allan
On 24/05/2011, at 6:46 PM, {microscopylistserver-noreply-at-microscopy.com} {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both ram-at-cen.dtu.dk as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: ram-at-cen.dtu.dk Name: Ramona } } Organization: DTU Cen } } Title-Subject: [Filtered] Sample prep of Pseudomonas Aeruginosa } } Message: Dear all, } I was wondering if any of you have experience with preparation of } Pseudomonas Aeruginosa samples grown on Agar plates for SEM. I am } interested in being able to have a bit of the Agar substrate with the } bacteria in the SEM. We are able to do LVSEM, ESEM, Peltier, Cryo SEM } and even TEM; so whatever protocol, article review and experience will } be appreciated :-) } Regards, } Ramona } } Login Host: 130.225.78.18 } --------------------------------------------------------------------------- }
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
Phone (03) 479 5642 or 479 7301 Fax (03) 479 5086 or 479 7254
EM Centre: http://ocem.otago.ac.nz/ Confocal Centre: http://occm.otago.ac.nz/ NZ Microscopy Society: http://microscopynz.co.nz/
==============================Original Headers============================== 17, 21 -- From allan.mitchell-at-stonebow.otago.ac.nz Tue May 24 04:08:20 2011 17, 21 -- Received: from mta03.xtra.co.nz (mta03.xtra.co.nz [210.54.141.252]) 17, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4O98Jjs013725 17, 21 -- for {microscopy-at-microscopy.com} ; Tue, 24 May 2011 04:08:20 -0500 17, 21 -- Received: from yom.mail.yahoo.net ([210.86.102.56]) by mta03.xtra.co.nz 17, 21 -- with ESMTP 17, 21 -- id {20110524090813.WBPK4106.mta03.xtra.co.nz-at-yom.mail.yahoo.net} ; 17, 21 -- Tue, 24 May 2011 21:08:13 +1200 17, 21 -- Subject: Re: [Microscopy] viaWWW: Sample prep of Pseudomonas Aeruginosa 17, 21 -- Mime-Version: 1.0 (Apple Message framework v1084) 17, 21 -- Content-Type: text/plain; charset=windows-1252 17, 21 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 17, 21 -- In-Reply-To: {201105240646.p4O6kRlp005231-at-ns.microscopy.com} 17, 21 -- Date: Tue, 24 May 2011 21:05:16 +1200 17, 21 -- Cc: microscopy-at-microscopy.com 17, 21 -- Message-Id: {5E2E5751-829D-496B-BE81-7DB4D03FF336-at-stonebow.otago.ac.nz} 17, 21 -- References: {201105240646.p4O6kRlp005231-at-ns.microscopy.com} 17, 21 -- To: ram-at-cen.dtu.dk 17, 21 -- X-Mailer: Apple Mail (2.1084) 17, 21 -- Content-Transfer-Encoding: 8bit 17, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4O98Jjs013725 ==============================End of - Headers==============================
Many thanks to everyone for taking the time to answer my question regarding the EBSD use. I received a lot of great answers. I will forward all the replies to the grad student and he might contact you in the future.
Thanks again. The listserve is truly a fantastic resource.
An engineering graduate student would like to use the EBSD system which is in our Zeiss Ultraplus thermal FESEM to get some data from his sample. He thinks EBSD is the best solution. However, I have no expertise in EBSD analysis, so any help in this matter will be very much appreciated. I have placed two powerpoint slides in my dropbox for people to view. Please click on the link below or cut and paste the URL on your web browser to see the diagram and what he wants to do with his sample.
http://dl.dropbox.com/u/7737489/EBSD.ppt
Thanks in advance.
Soumitra
************************************************************ Soumitra Ghoshroy Director, Electron Microscopy Center Research Professor, Biology University of South Carolina 715 Sumter Street Columbia, SC 29208 803-777-7085Â (office) 803-777-8908Â (fax) http://www.emc.sc.edu
==============================Original Headers============================== 6, 30 -- From rintugr-at-gmail.com Thu May 19 13:39:59 2011 6, 30 -- Received: from mail-pv0-f169.google.com (mail-pv0-f169.google.com [74.125.83.169]) 6, 30 -- Â Â Â Â by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4JIdxrr010911 6, 30 -- Â Â Â Â for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 13:39:59 -0500 6, 30 -- Received: by pvc12 with SMTP id 12so1816760pvc.0 6, 30 -- Â Â Â Â for {Microscopy-at-microscopy.com} ; Thu, 19 May 2011 11:39:59 -0700 (PDT) 6, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 30 -- Â Â Â Â d=gmail.com; s=gamma; 6, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 6, 30 -- Â Â Â Â Â :content-type; 6, 30 -- Â Â Â Â bh=5L+hroCC1MvBYJuB7cPfghGsviAX9KyFpG/SG/5hlnM=; 6, 30 -- b=JT7ANogLtumQwNONWtitamGm6+fQBBZ0ZI9yIEkrMzCuDLd9kLGIyhn0OSazWEe0Da 6, 30 -- AjaQInyhFuYcEkCynIK7xgwFBd89h24ltelKQia4Q+4jQlkaSV6ZFITBz7pCQO49UunU 6, 30 -- Â Â Â Â Â EbE7CdaJT1yMUrJf4w0mPtByZ83TJiGEUVkzQ= 6, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 30 -- Â Â Â Â d=gmail.com; s=gamma; 6, 30 -- Â Â Â Â h=mime-version:date:message-id:subject:from:to:content-type; 6, 30 -- b=KE7JCfV6MnFvdsKDU4ZdeH8g0aibPGPtkxz5DW/MBi2xUEc39+hFBZSVhiV3vjN96f 6, 30 -- iBp/ckXnxSaHcMYFARP856j1VmYFgQPtBSwsZm+ei1ItsY9egKdEeHyWOYyMtdvdEp/g 6, 30 -- Â Â Â Â Â JTNXywqWiLs4Zzggi1AbzOverJ1jmnpUb7O+M= 6, 30 -- MIME-Version: 1.0 6, 30 -- Received: by 10.142.229.18 with SMTP id b18mr1991430wfh.201.1305830398893; 6, 30 -- Â Thu, 19 May 2011 11:39:58 -0700 (PDT) 6, 30 -- Received: by 10.142.221.17 with HTTP; Thu, 19 May 2011 11:39:58 -0700 (PDT) 6, 30 -- Date: Thu, 19 May 2011 14:39:58 -0400 6, 30 -- Message-ID: {BANLkTim2tdYHV8iyEiVb8SFV8_kP=sC8Mg-at-mail.gmail.com} 6, 30 -- Subject: EBSD question 6, 30 -- From: Soumitra Ghoshroy {rintugr-at-gmail.com} 6, 30 -- To: Microscopy-at-microscopy.com 6, 30 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 33 -- From rintugr-at-gmail.com Tue May 24 09:53:14 2011 20, 33 -- Received: from mail-pw0-f41.google.com (mail-pw0-f41.google.com [209.85.160.41]) 20, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4OErC1W004241 20, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 24 May 2011 09:53:13 -0500 20, 33 -- Received: by pwi12 with SMTP id 12so4136016pwi.0 20, 33 -- for {Microscopy-at-microscopy.com} ; Tue, 24 May 2011 07:53:11 -0700 (PDT) 20, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 20, 33 -- d=gmail.com; s=gamma; 20, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 20, 33 -- :content-type:content-transfer-encoding; 20, 33 -- bh=8O5P7MQIMdVb8pl1wfpkOL0EigCu0kX8AzFJVdWA+LE=; 20, 33 -- b=GY6Vv+ves9R+dvCwT+hg/tDCj/tE2LPl3sOqdZ4R9w6Eqe4dWeSt8MGjEhKMrmK5Ra 20, 33 -- RLDpdB6HDyDJuZsxXnAFq0FpujvevHS65d1ntwP5jDGm5DsH20mWlYT1rG2DMbOU9foM 20, 33 -- PCZxvYAk483C6BpjcoVLiWCeSSSP2W5ZGWEgE= 20, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 20, 33 -- d=gmail.com; s=gamma; 20, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 20, 33 -- :content-transfer-encoding; 20, 33 -- b=ED1VPV+yJavugdAxVaJBuV0P9+hIc6+wVQORbP9rwok1if6kVhjzOiS6eIonofOFVE 20, 33 -- 4EvJWqnyTRNnuYoyMXBRiDpncCrxvDATfmHWCdCMcA9Upq+9KT9lirBC6GCJb4SqXDMq 20, 33 -- 03izJ/qkkPsK7NNrZHPpk51PYZrfjB9MlsYcQ= 20, 33 -- MIME-Version: 1.0 20, 33 -- Received: by 10.142.229.18 with SMTP id b18mr1105130wfh.201.1306248791787; 20, 33 -- Tue, 24 May 2011 07:53:11 -0700 (PDT) 20, 33 -- Received: by 10.142.221.17 with HTTP; Tue, 24 May 2011 07:53:11 -0700 (PDT) 20, 33 -- Date: Tue, 24 May 2011 10:53:11 -0400 20, 33 -- Message-ID: {BANLkTi=04A6gg7T0i3fEfUpGBcZiXPCYDg-at-mail.gmail.com} 20, 33 -- Subject: [Microscopy] EBSD question, Thank you 20, 33 -- From: Soumitra Ghoshroy {rintugr-at-gmail.com} 20, 33 -- To: Microscopy-at-microscopy.com 20, 33 -- Content-Type: text/plain; charset=UTF-8 20, 33 -- Content-Transfer-Encoding: 8bit 20, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4OErC1W004241 ==============================End of - Headers==============================
Your system may, in fact, be pumped down. If you have a turbo pumped system and the turbo is at full speed, then you have high vacuum. We have a cold cathode system in one of our systems that we sell. This is a Penning gauge. Every once in a while, a customer will call and tell us that the system is not going below a certain value. The gauge that we have is actually a dual gauge with a cross over point. The value that it is reading is the minimum pressure that the high pressure gauge can read. This sounds similar to what you are experiencing. There is a cure that the manufacturer of the gauge gave us that almost always works.
Take the gauge off of the system. Holding the gauge firmly in your hand, strike the gauge hard onto a telephone book hard several times with the flange of the gauge hitting the book. We really whack it to do this.
Why does it work? The Penning cell is actually sputtering material inside the gauge. Sometimes a flake can drop across the collector or short out the high voltage. Whacking it dislodges the flake. With the higher pressures that the EVO system can operate at, you will have more sputtering in the gauge that you will have at lower pressures.
You better check with the manufacturer prior to doing this procedure.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-----Original Message----- X-from: piper1960-at-hotmail.com [mailto:piper1960-at-hotmail.com] Sent: Friday, May 20, 2011 1:33 PM To: swalck-at-southbaytech.com
Hi All,
Sorry if you received the message twice.
On our Zeiss instrument (just over 1 year old) during pump down, the instrument gets to 9 Pa and progress seems to stop. Under "Vac status" it says "Waiting Penning." There were no error messages when initializing the software, and the green light on the Penning gauge is on. I am no expert, but I suspect that the vacuum system has pumped down as it should but the system can't read any signal from the Penning gauge and it won't allow the gun to fire.
This was the first time the tool has been started since the terrible weather we had in Alabama on April 27th and we lost power for several hours (it was on Standby when the power went out). I have turned off the tool and restarted it several times, but always with the same problem. Again, there appears to be no damage and there are no error messages.
Any suggestions on what the problem may be would be greatly appreciated.
Thank you,
Mark Puckett
T. Markham Puckett, Ph.D.
Associate Professor of Geology
Department of Physics and Earth Science
P.O. Box 5130
University of North Alabama
Florence, Alabama 35632-0001
"Why, sometimes I've believed as many as six impossible things before breakfast." The White Queen to Alice, Through the Looking-Glass
==============================Original Headers============================== 24, 18 -- From piper1960-at-hotmail.com Fri May 20 15:27:19 2011 24, 18 -- Received: from col0-omc3-s2.col0.hotmail.com (col0-omc3-s2.col0.hotmail.com [65.55.34.140]) 24, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4KKRJgx004869 24, 18 -- for {microscopy-at-microscopy.com} ; Fri, 20 May 2011 15:27:19 -0500 24, 18 -- Received: from COL117-W42 ([65.55.34.137]) by col0-omc3-s2.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 24, 18 -- Fri, 20 May 2011 13:27:18 -0700 24, 18 -- Message-ID: {COL117-W4241347A690ADF9FE34529BF710-at-phx.gbl} 24, 18 -- X-Originating-IP: [204.29.102.55] 24, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} 24, 18 -- To: {microscopy-at-microscopy.com} 24, 18 -- Subject: Penning gauge on Zeiss EVO MA10 24, 18 -- Date: Fri, 20 May 2011 15:27:18 -0500 24, 18 -- Importance: Normal 24, 18 -- Content-Type: text/plain; charset="Windows-1252" 24, 18 -- MIME-Version: 1.0 24, 18 -- X-OriginalArrivalTime: 20 May 2011 20:27:18.0777 (UTC) FILETIME=[50C1A690:01CC172C] 24, 18 -- Content-Transfer-Encoding: 8bit 24, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4KKRJgx004869 ==============================End of - Headers==============================
==============================Original Headers============================== 41, 23 -- From swalck-at-southbaytech.com Tue May 24 11:40:19 2011 41, 23 -- Received: from relay02.pair.com (relay02.pair.com [209.68.5.16]) 41, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4OGeJvD022490 41, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 May 2011 11:40:19 -0500 41, 23 -- Received: (qmail 80714 invoked from network); 24 May 2011 16:40:17 -0000 41, 23 -- Received: from 99.154.21.202 (HELO swalckPC) (99.154.21.202) 41, 23 -- by relay02.pair.com with SMTP; 24 May 2011 16:40:17 -0000 41, 23 -- X-pair-Authenticated: 99.154.21.202 41, 23 -- From: "Scott Walck" {swalck-at-southbaytech.com} 41, 23 -- To: {microscopy-at-microscopy.com} 41, 23 -- Cc: {piper1960-at-hotmail.com} 41, 23 -- References: {201105202032.p4KKWccA019992-at-ns.microscopy.com} 41, 23 -- In-Reply-To: {201105202032.p4KKWccA019992-at-ns.microscopy.com} 41, 23 -- Subject: RE: [Microscopy] Penning gauge on Zeiss EVO MA10 41, 23 -- Date: Tue, 24 May 2011 09:44:47 -0700 41, 23 -- Message-ID: {006101cc1a31$e4e0d000$aea27000$-at-com} 41, 23 -- MIME-Version: 1.0 41, 23 -- Content-Type: text/plain; 41, 23 -- charset="us-ascii" 41, 23 -- Content-Transfer-Encoding: 7bit 41, 23 -- X-Mailer: Microsoft Office Outlook 12.0 41, 23 -- Thread-Index: AcwXLQ+fZVLziwtTSFOhLxB9FuvxLgDAyMnw 41, 23 -- Content-language: en-us ==============================End of - Headers==============================
I fully agree with Ken - an all-inclusive (parts and labor) service contract, priced around 10% of the new instrument cost, is about right to keep most FIBs and SEMs running at OEM-spec level virtually indefinitely, but surely for 10+ years after the formally-declared EOL. Cost may get higher by 2% - 3% if the instrument is *very* old (20+ years) or if the customer with a single tool is located very remotely from the service organization. It is also reasonable for customer to expect that if FIB/SEM tool is covered by all-inclusive service contract, then its consumable parts should be provided at significant discount comparatively to the open-market prices.
The obvious dilemma "do I keep spending money on servicing this tool, or do I run it to ground and get a new one in a couple of years" is a business decision which everyone makes based on his/her/institution financial situation.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
The 10% rule would value a service contract at $50K- $60K to maintain an SEM with a purchase price of $500K - $600K. That sounds somewhat unjustifiably high assuming your proximity to the service technician doesn't entail extensive travel time/costs. In my opinion the 10% formula doesn't track very well as the purchase price of the instrument increases from entry level to "top of the line". I have maintained 2 somewhat costly FESEMs over the past 20+ years and in my experience their service history and cost of ownership don't support the 10% rule. The cost to maintain a high end system is only marginally higher than a less costly entry level model yet the 10% rule could easily double the service contract price.
John A. Robson
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Tuesday, May 24, 2011 11:17 PM To: Robson,John (AN) BIP-US-R
Catching up with e-mail....
I fully agree with Ken - an all-inclusive (parts and labor) service contract, priced around 10% of the new instrument cost, is about right to keep most FIBs and SEMs running at OEM-spec level virtually indefinitely, but surely for 10+ years after the formally-declared EOL. Cost may get higher by 2% - 3% if the instrument is *very* old (20+ years) or if the customer with a single tool is located very remotely from the service organization. It is also reasonable for customer to expect that if FIB/SEM tool is covered by all-inclusive service contract, then its consumable parts should be provided at significant discount comparatively to the open-market prices.
The obvious dilemma "do I keep spending money on servicing this tool, or do I run it to ground and get a new one in a couple of years" is a business decision which everyone makes based on his/her/institution financial situation.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/17/2011 9:00 AM, kenconverse-at-qualityimages.biz wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Rosemary, } Your confocal sounds about right, although with only about a 25% increase } over 10 years, I think you can count your blessings. } } As for your ESEM, I should think the terms should look very much like your } confocal contract. Are they claiming that all the break-downs were caused } by mis-use and abuse? If that were the case, they shouldn't have covered } the repairs even under warranty. On the other hand, if the failures were } due to their poor design and construction, they certainly have a lot of } nerve implying that your contract is being increased because of that. I'm } also very surprised that parts are not covered. To me, that is not a } service contract, particularly for a new instrument. It can be } understandable for very old instruments, but should be reflected in the } pricing. } } Whoever the vendor is, I would certainly have a hard time recommending them } to anyone, given the situation you describe. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: rosemary.white-at-csiro.au [mailto:rosemary.white-at-csiro.au] } Sent: Monday, May 16, 2011 10:01 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear all, } } Just to get some numbers here, how much is reasonable for a service } contract? 10% of original cost of the instrument? 15% of cost? } } We have a service contract on one instrument (confocal) that was (10 years } ago) about 8% of original cost, now is about 10%. This is essentially a } full extended warranty, and includes an annual service visit, unlimited } emergency callouts and all replacement parts, even lasers are included. } It's been great. I don't think they have this type of contract for new } instruments, though(c) } } However, for a new instrument (ESEM) from a different supplier, we were } quoted about 15% of the original cost for a contract with 2 annual } maintenance visits plus unlimited emergency callouts, but no coverage of } replacement parts. For 2 maintenance visits and 2 emergency callouts but } no parts the cost went down to about 8% of initial cost. The reason given } for the cost was that we had many breakdowns in the 12 month warranty } period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, } computer fan blew up, etc, etc), and they had to factor this in. } Considering the presumed reliability of SEMs compared to confocals (in my } experience before this new instrument), and that no parts were covered, it } seemed quite expensive. } } Is the latter about what you'd expect to pay for a service contract? } } Thanks, } Rosemary White } } Dr Rosemary White } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au } } } } } } } ==============================Original Headers============================== } 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 } 2011 } 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au } [150.229.7.36]) } 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p4H1wX9q015257 } 13, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 } -0500 } 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; } 13, 32 -- s=email; t=1305597514; x=1337133514; } 13, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: } 13, 32 -- content-transfer-encoding; } 13, 32 -- bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; } 13, 32 -- b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv } 13, 32 -- VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb } 13, 32 -- +BsypGn+/TtdMUH; } 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; } 13, 32 -- d="scan'208";a="20957752" } 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) } 13, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May 2011 } 11:58:32 +1000 } 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by } 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP } Server } 13, 32 -- (TLS) id 8.3.159.2; Tue, 17 May 2011 11:58:32 +1000 } 13, 32 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 } 13, 32 -- Date: Tue, 17 May 2011 11:58:29 +1000 } 13, 32 -- Subject: Re: Microscope service strategies - numbers } 13, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} } 13, 32 -- To: {Microscopy-at-microscopy.com} } 13, 32 -- Message-ID: {C9F81355.10B35%rosemary.white-at-csiro.au} } 13, 32 -- Thread-Topic: Microscope service strategies - numbers } 13, 32 -- In-Reply-To: {201105162121.p4GLLsvq019450-at-ns.microscopy.com} } 13, 32 -- MIME-Version: 1.0 } 13, 32 -- Content-Type: text/plain; charset="ISO-8859-1" } 13, 32 -- Content-Transfer-Encoding: 8bit } 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p4H1wX9q015257 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 26, 28 -- From kenconverse-at-qualityimages.biz Tue May 17 07:59:16 2011 } 26, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) } 26, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4HCxFtC012444 } 26, 28 -- for {microscopy-at-microscopy.com} ; Tue, 17 May 2011 07:59:16 -0500 } 26, 28 -- X-Authority-Analysis: v=1.1 cv=2eRU7/Hz3VlqkdLxzKFKJPBwNf5HXLm+W19RdYpLufc= c=1 sm=0 a=qFnv_w0bjHcA:10 a=-CRmgG0JhlAA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=XnRPBOH4DihgOh83M_YA:9 a=pt6S-TXmAZJB4dK-i18A:7 a=jiObf9B0YAUA:10 a=5JUlSvKOtR0A:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=G91thX30KR8A:10 a=wi6guWX3a2tTbfmz:21 a=XBpoec4P42n-xxcn:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 26, 28 -- X-Cloudmark-Score: 0 } 26, 28 -- X-Originating-IP: 72.224.149.57 } 26, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1416] helo=Ken) } 26, 28 -- by cdptpa-oedge03.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) } 26, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP } 26, 28 -- id 84/2B-03125-32172DD4; Tue, 17 May 2011 12:59:15 +0000 } 26, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 26, 28 -- To: {rosemary.white-at-csiro.au} , "MSA Listserver" {microscopy-at-microscopy.com} } 26, 28 -- Subject: RE: [Microscopy] Re: Microscope service strategies - numbers } 26, 28 -- Date: Tue, 17 May 2011 08:59:11 -0400 } 26, 28 -- Message-ID: {E9C2B6DDE3574F998FE24A44FCB9D732-at-Ken} } 26, 28 -- MIME-Version: 1.0 } 26, 28 -- Content-Type: text/plain; } 26, 28 -- charset="iso-8859-2" } 26, 28 -- X-Priority: 3 (Normal) } 26, 28 -- X-MSMail-Priority: Normal } 26, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 26, 28 -- Importance: Normal } 26, 28 -- Thread-Index: AcwUNkEb7efDY/ljTW6jHrG8pRn0NQAWhmew } 26, 28 -- In-Reply-To: {201105170200.p4H20s4F018750-at-ns.microscopy.com} } 26, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 } 26, 28 -- Content-Transfer-Encoding: 8bit } 26, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4HCxFtC012444 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 46 -- From vray-at-partbeamsystech.com Tue May 24 22:07:27 2011 6, 46 -- Received: from nm15-vm0.bullet.mail.sp2.yahoo.com (nm15-vm0.bullet.mail.sp2.yahoo.com [98.139.91.208]) 6, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4P37RUK017376 6, 46 -- for {microscopy-at-microscopy.com} ; Tue, 24 May 2011 22:07:27 -0500 6, 46 -- Received: from [98.139.91.62] by nm15.bullet.mail.sp2.yahoo.com with NNFMP; 25 May 2011 03:07:26 -0000 6, 46 -- Received: from [98.139.91.51] by tm2.bullet.mail.sp2.yahoo.com with NNFMP; 25 May 2011 03:07:26 -0000 6, 46 -- Received: from [127.0.0.1] by omp1051.mail.sp2.yahoo.com with NNFMP; 25 May 2011 03:07:26 -0000 6, 46 -- X-Yahoo-Newman-Id: 943886.66614.bm-at-omp1051.mail.sp2.yahoo.com 6, 46 -- Received: (qmail 46035 invoked from network); 25 May 2011 03:07:26 -0000 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1306292846; bh=D5Ev/Ntxbs5VEOqIkNczW5zkgBv4b7HtanNlShi3cOo=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date:F rom:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:References:In-Re ply-To:Content-Type:Content-Transfer-Encoding; b=LG6vIB8mmPiJmvgMTpmFpCWEB+p2eYs9jElalsCBE8p3tCkyqB3jSAt4TJy6Ez8fR4Kx76xP3dv 80p0tHCN9Tz2LB9WXnLTaWa+FHlgu6hGo0+TKSIJyeHbCFgNIe0DO7nOIhGGspDtTxyZF4r6lJ+ls uAXpI7OogwRi4yiWCaQ= 6, 46 -- Received: from [192.168.2.31] (vray-at-75.68.231.25 with plain) 6, 46 -- by smtp114.biz.mail.sp1.yahoo.com with SMTP; 24 May 2011 20:07:26 -0700 PDT 6, 46 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 46 -- X-YMail-OSG: E62VMggVM1nbRm268.ukeOY0zQOFAZhod5B5RZexxE2HP7Z 6, 46 -- pQt19YXV_p6wAVnv8AXX5rhEIZmRpRGyfG9jYpZlX_aXtervAzX58mQEeXxn 6, 46 -- jfIfGe0QRjgfFsI8KYdGARQTMbFNgjWoCrEANA_4y.IBOlBF0ZZ3dK49hGfm 6, 46 -- ahEtNoqn3eH.Q5oOG63QX_WooAYdBowRnWjCcVr83IFGJk2CTpZZBKwg0m3B 6, 46 -- HkvMbCfofO7dqj6i353OlbdcR8U1OEa3aO.EiB4rWmMUlSDWwYl2vT1rM9ZI 6, 46 -- Yowv6Ts2.gxutuPdzQJMpL4RjcTTuz0RBH8FpwCCfQAFlYa_7Y2JQZ8b_DZ_ 6, 46 -- fw4BFwMUB4UOgQFvl7ryK7ZdmAwzkI1DdPMeVw.plr8MGnbGml9nfnsJ3B2h 6, 46 -- 7ZwgP0CePWo1mtpjQA.MYdB2Q2VnnVn5YYHRrPqFWNF5rACpZrOhofkAcSkc 6, 46 -- R7fdzxRKEm5CKW5KYmjQscQjuKC4lMArzZvLdAPt8ZDj31anYT5Sldl8EO8K 6, 46 -- ilNxWhY8OVtz_ayC8n2DLQHxB01cJyXjImQiqKC.2EsAm8LzkC4TeUaH.6wM 6, 46 -- sChcLqmdHu_VgnVeisBomlhauhSjG0l7AAV8zX2ud88D97X5M5dD7JNTGChN 6, 46 -- ghtf0IixqoNvbEyeFBNrq0Fhqtno4ykjlyeX3CWdahp188ULAad08wVJFi9x 6, 46 -- 0Zn0gR8PXbNYfvKYO.V6P3C2kdYyFufeDNtj3EValBBMrklEvmSFPMySTWdm 6, 46 -- jst3jSjio8Uz9Mj37zpD0UnF__rRHJ_6.03_.SYfjTHT.1CAFLJ73UjHv49R 6, 46 -- _.ZAejL6_UCrQ6P26qAtaWtX_yJTwsv2Ikbo4gFU7i0WM_8mtEy8HevtdDxG 6, 46 -- V4xBtPgnmvswgsaT8AhdCorgTFdF15RRTmSksBQ8EFaF8WJVKZyGfY2Ts8St 6, 46 -- d1y8RS122mKw1P_l5gZBXWcbDuTZgVp03r6ftf8aQYNrhrdOuRU0Kt4zhLi0 6, 46 -- rBHtySgSpuGGw_FEzROpuKp.s1C9JPnkcAirmBpkJXQeNzRNWf6vBnXFgZ32 6, 46 -- NMZyCtt4- 6, 46 -- X-Yahoo-Newman-Property: ymail-3 6, 46 -- Message-ID: {4DDC726D.2070606-at-partbeamsystech.com} 6, 46 -- Date: Tue, 24 May 2011 23:07:25 -0400 6, 46 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 6, 46 -- Reply-To: vray-at-partbeamsystech.com 6, 46 -- Organization: PBS&T 6, 46 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 6, 46 -- MIME-Version: 1.0 6, 46 -- To: kenconverse-at-qualityimages.biz, microscopy-at-microscopy.com 6, 46 -- Subject: Re: [Microscopy] Microscope service strategies - numbers 6, 46 -- References: {201105171300.p4HD0Ttk013785-at-ns.microscopy.com} 6, 46 -- In-Reply-To: {201105171300.p4HD0Ttk013785-at-ns.microscopy.com} 6, 46 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 46 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 15, 28 -- From prvs=1199275cb=john.robson-at-boehringer-ingelheim.com Wed May 25 09:50:09 2011 15, 28 -- Received: from mail4.boehringer-ingelheim.com (mail4.boehringer-ingelheim.com [148.188.192.93]) 15, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4PEo9iA004738 15, 28 -- for {microscopy-at-microscopy.com} ; Wed, 25 May 2011 09:50:09 -0500 15, 28 -- Received: from nahexb01.am.boehringer.com ([10.128.98.121]) by RDGEXH01.boehringer.com with Microsoft SMTPSVC(6.0.3790.4675); 15, 28 -- Wed, 25 May 2011 10:50:08 -0400 15, 28 -- Received: from nahexm105.am.boehringer.com ([10.128.98.84]) by nahexb01.am.boehringer.com with Microsoft SMTPSVC(6.0.3790.4675); 15, 28 -- Wed, 25 May 2011 10:50:08 -0400 15, 28 -- x-mimeole: Produced By Microsoft Exchange V6.5 15, 28 -- Content-class: urn:content-classes:message 15, 28 -- MIME-Version: 1.0 15, 28 -- Content-Type: text/plain; 15, 28 -- charset="us-ascii" 15, 28 -- Subject: RE: [Microscopy] Re: Microscope service strategies - numbers 15, 28 -- Date: Wed, 25 May 2011 10:49:14 -0400 15, 28 -- Message-ID: {18F7E98C5EF4664BBBD1144AFE158512620564-at-nahexm105.am.boehringer.com} 15, 28 -- In-Reply-To: {201105250316.p4P3Ge3i031357-at-ns.microscopy.com} 15, 28 -- X-MS-Has-Attach: 15, 28 -- X-MS-TNEF-Correlator: 15, 28 -- Thread-Topic: [Microscopy] Re: Microscope service strategies - numbers 15, 28 -- Thread-Index: AcwaiisFKr6O9ReMTFq5nhuInzYo+AAWnvxA 15, 28 -- References: {201105250316.p4P3Ge3i031357-at-ns.microscopy.com} 15, 28 -- From: {john.robson-at-boehringer-ingelheim.com} 15, 28 -- To: {microscopy-at-microscopy.com} 15, 28 -- Cc: {vray-at-partbeamsystech.com} 15, 28 -- X-OriginalArrivalTime: 25 May 2011 14:50:08.0292 (UTC) FILETIME=[0A835E40:01CC1AEB] 15, 28 -- Content-Transfer-Encoding: 8bit 15, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4PEo9iA004738 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-med.wayne.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Immunogold with SEM
Message: Does anyone know of literature references or vendor technical notes that mentions labelling of surface antigens on cells in tissue explants? I am looking for large probes (~ 100 nm)for SEM studies. Thanks, Vickie
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] Ultramicrotome service
Message: Hi, I am looking for a service for our Reichert-Jung Ultracut E microtome in Maritime area, Canada. Our ultramicrotomes are in need of a tune up or more. Thank you Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both david.springett-at-spotimaging.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: david.springett-at-spotimaging.com Name: David Springett
Message: We have an opening in our Florida territory for a Sales Specialist - selling digital cameras, grossing imaging systems and microscopes. Experience in the technology and in sales preferred.
If you are interested please contact me. Thank you
I would recommend using nanogold labeled probes followed by gold enhancement. The Nanoprobes kit worked great for us - see: Meagher, C.K., H. Liu, C.P. Moore, and T.E. Phillips. 2005. Conjunctival M cells selectively bind and translocate Maackia amurensis leukoagglutinin. Experimental Eye Research= 80(4):545-553.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
The College of Nanoscale Science and Engineering (CNSE) seeks to hire a research scientist in the area of aberration-corrected transmission electron microscopy (TEM). Duties will include, but not be limited to: managing the FEI Titan TEM facility, and working closely with the CNSE faculty, staff and strategic partners to develop new methodologies for nanomaterials analysis and semiconductor metrology. Effort will be split between advanced applications support and advanced technique development.
Thomas M. Murray, Ph.D. Senior Research Support Specialist / Instructor College of Nanoscale Science & Engineering University at Albany-SUNY 251 Fuller Rd. Albany, NY 12203
==============================Original Headers============================== 6, 30 -- From mullarkay-at-gmail.com Wed May 25 12:30:04 2011 6, 30 -- Received: from mail-yw0-f41.google.com (mail-yw0-f41.google.com [209.85.213.41]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4PHU3ZC023314 6, 30 -- for {microscopy-at-microscopy.com} ; Wed, 25 May 2011 12:30:04 -0500 6, 30 -- Received: by ywa1 with SMTP id 1so3941360ywa.0 6, 30 -- for {microscopy-at-microscopy.com} ; Wed, 25 May 2011 10:30:03 -0700 (PDT) 6, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 30 -- d=gmail.com; s=gamma; 6, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 6, 30 -- :content-type; 6, 30 -- bh=e/8S9/IApPDQvtdwkzP62uQmXAM+T34rqJgcfSEIMjQ=; 6, 30 -- b=IN5kAi/onFjGkMXLaYFtubTKr5widD+ODv3uLupiWjQnrR1m/+n/nKJl+40WdFI/dW 6, 30 -- 2UVpKAn7zx9AUbyEbEfwA8ylZzW6cwuAbeZQ3qakcMd2YZbUvC+SoPCnSocFx+T0MTl0 6, 30 -- 3BkDGov7nVHD/CZyTUiA3DYjv8xnLbn9/5NTk= 6, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 6, 30 -- d=gmail.com; s=gamma; 6, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 6, 30 -- b=PyaUMwt7q3JwSX8z0nsUQ2OHGx+dDf8gHdXJObiW45a48Au88BJc3SicZq/DbgM00y 6, 30 -- +REl/0vY0+KZU5mMXTbuaPLpWc+SFu7WMBgZypl8dU+7mJKjIgVd5aBgQ4o9YEfBY96m 6, 30 -- fak67wEobXYVHIgIc8SHHyZgDX/7xGKtZ921A= 6, 30 -- MIME-Version: 1.0 6, 30 -- Received: by 10.100.241.13 with SMTP id o13mr3977731anh.143.1306344602940; 6, 30 -- Wed, 25 May 2011 10:30:02 -0700 (PDT) 6, 30 -- Received: by 10.101.1.17 with HTTP; Wed, 25 May 2011 10:30:02 -0700 (PDT) 6, 30 -- Date: Wed, 25 May 2011 13:30:02 -0400 6, 30 -- Message-ID: {BANLkTi=HCU3wxBdHUdWEJUB+fSxqEb+UGA-at-mail.gmail.com} 6, 30 -- Subject: TEM Job Opening 6, 30 -- From: Thomas Murray {mullarkay-at-gmail.com} 6, 30 -- To: microscopy-at-microscopy.com 6, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
The 10% rule applies to all-inclusive support by external organization and must sustain existence of such service organization, cover all costs, risks, and provide reasonable profit. The amount of $50K - $60K per year are about right, it will be hard to sustain business of the support organization otherwise.
Of cause, you can do self-service and keep tools running at much lower costs: for a while, on some instruments, and with higher risks. That is a business decision made by many and it may be justified in your particular situation. Just understand clearly what are the risks that you are taking and accept the fact that whatever happens you are (or your institution) will be on your (its) own.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/25/2011 10:49 AM, john.robson-at-boehringer-ingelheim.com wrote: } The 10% rule would value a service contract at $50K- $60K to maintain an SEM } with a purchase price of $500K - $600K. That sounds somewhat unjustifiably } high assuming your proximity to the service technician doesn't entail } extensive travel time/costs. In my opinion the 10% formula doesn't track } very well as the purchase price of the instrument increases from entry level } to "top of the line". I have maintained 2 somewhat costly FESEMs over the } past 20+ years and in my experience their service history and cost of } ownership don't support the 10% rule. The cost to maintain a high end system } is only marginally higher than a less costly entry level model yet the 10% } rule could easily double the service contract price. } } } John A. Robson } } -----Original Message----- } From: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } Sent: Tuesday, May 24, 2011 11:17 PM } To: Robson,John (AN) BIP-US-R } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Catching up with e-mail.... } } I fully agree with Ken - an all-inclusive (parts and labor) service } contract, priced around 10% of the new instrument cost, is about right } to keep most FIBs and SEMs running at OEM-spec level virtually } indefinitely, but surely for 10+ years after the formally-declared EOL. } Cost may get higher by 2% - 3% if the instrument is *very* old (20+ } years) or if the customer with a single tool is located very remotely } from the service organization. It is also reasonable for customer to } expect that if FIB/SEM tool is covered by all-inclusive service } contract, then its consumable parts should be provided at significant } discount comparatively to the open-market prices. } } The obvious dilemma "do I keep spending money on servicing this tool, or } do I run it to ground and get a new one in a couple of years" is a } business decision which everyone makes based on his/her/institution } financial situation. } } Cheers :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } } On 5/17/2011 9:00 AM, kenconverse-at-qualityimages.biz wrote: } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hi Rosemary, } } Your confocal sounds about right, although with only about a 25% increase } } over 10 years, I think you can count your blessings. } } } } As for your ESEM, I should think the terms should look very much like your } } confocal contract. Are they claiming that all the break-downs were caused } } by mis-use and abuse? If that were the case, they shouldn't have covered } } the repairs even under warranty. On the other hand, if the failures were } } due to their poor design and construction, they certainly have a lot of } } nerve implying that your contract is being increased because of that. I'm } } also very surprised that parts are not covered. To me, that is not a } } service contract, particularly for a new instrument. It can be } } understandable for very old instruments, but should be reflected in the } } pricing. } } } } Whoever the vendor is, I would certainly have a hard time recommending them } } to anyone, given the situation you describe. } } } } Ken Converse } } owner } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } -----Original Message----- } } X-from: rosemary.white-at-csiro.au [mailto:rosemary.white-at-csiro.au] } } Sent: Monday, May 16, 2011 10:01 PM } } To: kenconverse-at-qualityimages.biz } } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Dear all, } } } } Just to get some numbers here, how much is reasonable for a service } } contract? 10% of original cost of the instrument? 15% of cost? } } } } We have a service contract on one instrument (confocal) that was (10 years } } ago) about 8% of original cost, now is about 10%. This is essentially a } } full extended warranty, and includes an annual service visit, unlimited } } emergency callouts and all replacement parts, even lasers are included. } } It's been great. I don't think they have this type of contract for new } } instruments, though(c) } } } } However, for a new instrument (ESEM) from a different supplier, we were } } quoted about 15% of the original cost for a contract with 2 annual } } maintenance visits plus unlimited emergency callouts, but no coverage of } } replacement parts. For 2 maintenance visits and 2 emergency callouts but } } no parts the cost went down to about 8% of initial cost. The reason given } } for the cost was that we had many breakdowns in the 12 month warranty } } period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, } } computer fan blew up, etc, etc), and they had to factor this in. } } Considering the presumed reliability of SEMs compared to confocals (in my } } experience before this new instrument), and that no parts were covered, it } } seemed quite expensive. } } } } Is the latter about what you'd expect to pay for a service contract? } } } } Thanks, } } Rosemary White } } } } Dr Rosemary White } } CSIRO Plant Industry } } GPO Box 1600 } } Canberra, ACT 2601 } } Australia } } } } T 61 2 6246 5475 } } F 61 2 6246 5334 } } E rosemary.white-at-csiro.au } } } } } } } } } } } } } } ==============================Original } Headers============================== } } 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 } } 2011 } } 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au } } [150.229.7.36]) } } 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p4H1wX9q015257 } } 13, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 } } -0500 } } 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } } 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; } } 13, 32 -- s=email; t=1305597514; x=1337133514; } } 13, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: } } 13, 32 -- content-transfer-encoding; } } 13, 32 -- bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; } } 13, 32 -- b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv } } 13, 32 -- VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb } } 13, 32 -- +BsypGn+/TtdMUH; } } 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; } } 13, 32 -- d="scan'208";a="20957752" } } 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) } } 13, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May } 2011 } } 11:58:32 +1000 } } 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by } } 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft
I appreciate your tips on best methods for TEM imaging of a C/Si material. It is prepared for me as a spread over a glass microscope slide. Will need to peel it off the slide for processing for thin sectioning TEM. It has been suggested that cooling/freezing and fracturing the material might be another method for thinning. Are there protocols that readers can guide me to for such samples?
Thanks so much,
Steve
-- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
==============================Original Headers============================== 6, 18 -- From steven.samuelsson-at-sri.com Wed May 25 19:23:07 2011 6, 18 -- Received: from mail1.sri.com (newmail.SRI.COM [128.18.30.17]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4Q0N7tt030431 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 25 May 2011 19:23:07 -0500 6, 18 -- MIME-version: 1.0 6, 18 -- Content-transfer-encoding: 7BIT 6, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 6, 18 -- Received: from Steves-iMac.local ([unknown] [128.18.51.102]) 6, 18 -- by mail.sri.com (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built 6, 18 -- Jul 30 2009)) with ESMTP id {0LLS00H5G12ISLD0-at-mail.sri.com} for 6, 18 -- Microscopy-at-microscopy.com; Wed, 25 May 2011 17:23:06 -0700 (PDT) 6, 18 -- Message-id: {4DDD9D6A.3060109-at-sri.com} 6, 18 -- Date: Wed, 25 May 2011 17:23:06 -0700 6, 18 -- From: Steven Samuelsson {steven.samuelsson-at-sri.com} 6, 18 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.2.17) 6, 18 -- Gecko/20110414 Thunderbird/3.1.10 6, 18 -- To: Microscopy-at-microscopy.com 6, 18 -- Subject: C/Si material for thin-X TEM ==============================End of - Headers==============================
Full-time Electron Microscopy Analyst and Microscopy Laboratory Supervisor position available at The Catholic University of America, Washington, DC
Position Summary: This position supports the research and teaching efforts of The Vitreous State Laboratory and the Physics Department by supervising the Scanning Electron Microscopy (SEM) facility, and by producing, reporting and filing data from SEM examination of specimens submitted by managers of various laboratory research projects. Energy Dispersive Spectroscopy (EDS), Wavelength Dispersive Spectroscopy (WDS), and image analysis are available and employed in specimen characterization. The majority of facility work is generated by our nuclear waste-glass research program. The position also involves the instruction of lab employees and students in the use of the SEM´s and associated equipment, of visible light-optical microscopes, of sample preparation equipment and techniques, and the development and maintenance of protocols for the safe use of the equipment and chemicals related to electron microscopy. The facility is responsible for the performing and evaluating the Vapor Hydration Test (VHT) (ASTM C1663-09) on selected candidate waste-glass compositions to evaluate their resistance to aqueous corrosion.
Description: Full-time permanent position.
Minimum Qualifications: Masters degree in Physics, Chemistry, Geology, Materials Science or a closely related field. A lesser degree may suffice with specialized education and work experience in microscopy. Work Experience: A minimum of 3 years experience in the operation and routine maintenance of electron microscopes, light-optical microscopes and metallographic sample preparation equipment. At least one year of supervisory experience, or demonstrated supervisory skills and experience in laboratory management and procedures. Transmission Electron Microscopy (TEM) skills and experience desirable.
Submit application and CV to Dr. Andrew Buechele buechele-at-cua.edu Vitreous State Laboratory 409 Hannan Hall Cardinal Station Washington, DC 20064
==============================Original Headers============================== 7, 20 -- From andrewb-at-vsl.cua.edu Wed May 25 20:17:03 2011 7, 20 -- Received: from mail.vsl.cua.edu (interface.vsl.cua.edu [136.242.188.2]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4Q1H3qU014760 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 May 2011 20:17:03 -0500 7, 20 -- Received: from vsl.cua.edu (ab-rm409.vsl.cua.edu [136.242.189.129]) 7, 20 -- by mail.vsl.cua.edu (Postfix) with ESMTPA id A294B4600BBDE 7, 20 -- for {Microscopy-at-Microscopy.Com} ; Wed, 25 May 2011 21:17:03 -0400 (EDT) 7, 20 -- From: "Andrew Buechele" {andrewb-at-vsl.cua.edu} 7, 20 -- To: Microscopy-at-Microscopy.Com 7, 20 -- Date: Wed, 25 May 2011 21:17:04 -0400 7, 20 -- MIME-Version: 1.0 7, 20 -- Subject: Microscopy Position avalilable at Catholic University of America 7, 20 -- Reply-to: andrewb-at-vsl.cua.edu 7, 20 -- Message-ID: {4DDD71D0.31448.1B31856-at-andrewb.vsl.cua.edu} 7, 20 -- Priority: normal 7, 20 -- X-mailer: Pegasus Mail for Windows (4.41) 7, 20 -- Content-type: text/plain; charset=ISO-8859-1 7, 20 -- Content-description: Mail message body 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from Quoted-printable to 8bit by ns.microscopy.com id p4Q1H3qU014760 ==============================End of - Headers==============================
There are references with the images. More images at: http://www.ansci.wisc.edu/facstaff/Faculty/pages/albrecht/albrecht_web/Programs/microscopy/gallery.html
Imaging colloidal-gold conjugated antibodies on cell surface antigens in the SEM is quite doable. Depending on the SEM. You do need a good low-voltage FESEM and a high quality backscatter detector, one that works well at low voltages.
A "standard" SEM could see 100 nm targets, but that is really huge for labelling surface antigens. There will be lots of problems with such a large probe. Even getting proper labelling will be an issue. Do you have access to a good field-emission SEM with a Good BSE detector? If yes, then you can use 10 nm or smaller particles, 18 nm particles easily. If you don't have this access, then you might want to think of a different approach.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both vakimler-at-med.wayne.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Wayne State University School of Medicine
Title-Subject: [Filtered] Immunogold with SEM
Message: Does anyone know of literature references or vendor technical notes that mentions labelling of surface antigens on cells in tissue explants? I am looking for large probes (~ 100 nm)for SEM studies. Thanks, Vickie
Question: Based upon what information, other than the 10% rule, have you arrived at the conclusion that $50k - %60 is a reasonable amount? I never mentioned which SEM I was running, the service history of the SEM / facility, or what materials were being examined? An across the board determination fails to consider several risks associated with each instrument.
I totally agree that a service organization needs to turn a profit and investors should have every expectation that they have made a wise investment. I also agree that there is a degree of uncertainty with regards to the liability associated with equipment failure. Costs associated with both technician time (and travel/housing/food costs) and replacement parts is a gamble for the service provider, although a larger customer base mitigates the risk. In fact a case could easily be made that lower cost service contracts with a higher customer base could lead to greater profits. However, my comments with regards to a sweeping across the board 10% rule actually goes to the financial risks associated with each type of instrument. Customers with high cost instruments, with only a slightly higher cost of maintenance, shouldn't bare the brunt of the service organizations profits. The price of a service contract should be determined by the actual costs to maintain the tool with a reasonable amount of profit tacked on to the bill. This does vary by instrument type and design but doesn't track very well with purchase price of the equipment. Suppose you're in a multi-user facility with a track record of heavy handed use leading to frequent repairs? Perhaps you work with materials that require frequent visits to clean up a messy column? Either situation could, and does, result in multiple years where service costs exceed 10%? Does the 10% rule still apply? I suppose if you're running the lab it sounds about right! :-)
However, going back to the original posting in this thread, it sounds like Rosemary White's instrument isn't very reliable and her facility is being extorted by the manufacturer. If I were in the market for a new ESEM I would contact her off line, you might want to get the name of that company! But it does go straight to the heart of my argument regarding "10%". Her service provider has crunched the numbers and for her particular failure prone ESEM they need to charge 15% to keep their organization going!
My experience has been that service contract costs, for the high ticket instruments (that work!), don't track with the 10% rule. And my service company has still managed to turn a profit from my site every year for 20 years.
John A. Robson
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Wednesday, May 25, 2011 5:37 PM To: Robson,John (AN) BIP-US-R Cc: microscopy-at-microscopy.com
Hello everyone, I need the collective wisdom of the list server: I have a badly cared for Zeiss Photomicroscope III (the universal stand, I believe), which I recently inherited.
Taking it apart I have discovered it appears to be set up for reflected fluorescence and transmitted phase. (It is missing the large Hg burner used for fluorescence.) It's a very cool scope, once set up for 35mm film, but I need a pol scope for particle identification. I also need a trinocular head.
My question is, is there anyone who refurbishes these scopes and can supply the needed parts to convert the scope to polarized light.
Thanks ................
Frank
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 11, 25 -- From frank_karl-at-ardl.com Thu May 26 09:21:10 2011 11, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4QEL9UF017516 11, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 26 May 2011 09:21:10 -0500 11, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 11, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 26 11, 25 -- May 2011 10:21:09 -0400 11, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 11, 25 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 11, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 11, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 11, 25 -- Date: Thu, 26 May 2011 10:21:07 -0400 11, 25 -- Subject: Zeiss photomicroscopeIII 11, 25 -- Thread-Topic: Zeiss photomicroscopeIII 11, 25 -- Thread-Index: AcwbsCdaAAJVCMWzRPm7p2ZK3Y64gg== 11, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077CD096A50-at-exchange2k7.ad.ardl.com} 11, 25 -- Accept-Language: en-US 11, 25 -- Content-Language: en-US 11, 25 -- X-MS-Has-Attach: 11, 25 -- X-MS-TNEF-Correlator: 11, 25 -- acceptlanguage: en-US 11, 25 -- Content-Type: text/plain; charset="iso-8859-1" 11, 25 -- MIME-Version: 1.0 11, 25 -- Content-Transfer-Encoding: 8bit 11, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4QEL9UF017516 ==============================End of - Headers==============================
About Pol Scopes: http://earth2geologists.net/Microscopes/ [with links] http://cgi.ebay.com/Professional-Zeiss-Polarization-Microscope-5-objectives-/140455050865 [ebay]
LED fluorescence: http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=2615&gclid=CMyuotj6hakCFUM65Qodvidgqw here you get the excitation you want with long life [I have no experience with LED UV, but I see it mor and more] http://www.viewsfromscience.com/documents/webpages/led_fluorescence.html [strange & homemade, but???] http://www.mightexsystems.com/family_info.php?cPath=187&categories_id=187
Google strings: {LED fluorescent microscope illuminators} ; and {used Zeiss microscopes} [quick search, NO hits, but I didn't need anything Zeiss} B&B in Pittsburgh [Olympus history, good folks]: http://www.bbmicro.com/index.php [NO direct relationship, we have Olympus confocal]
If what you NEED is polarizing optics, then perhaps you can work a trade of what you don't need. I admit the Zeiss is a wonderful system, but it appears to be what you do not need, thus, it has only for which you may need only an accessory.
Khowing that bad advice (as a Biologist, I would NEVER get rid of such a good instrument!!) is almost always free, I humbly submit the above for your consideration.
Cheers from a Leitz Ortholux with Ploem fluorescence user,
Fred Monson Technical Director
CMIRT, West Chester University West Chester, PA, 19383 610-738-0437 http://cmirt.wcupa.edu ============================================================
From: frank_karl-at-ardl.com [frank_karl-at-ardl.com] Sent: Thursday, May 26, 2011 10:28 AM To: Monson, Frederick
Hello everyone, I need the collective wisdom of the list server: I have a badly cared for Zeiss Photomicroscope III (the universal stand, I believe), which I recently inherited.
Taking it apart I have discovered it appears to be set up for reflected fluorescence and transmitted phase. (It is missing the large Hg burner used for fluorescence.) It's a very cool scope, once set up for 35mm film, but I need a pol scope for particle identification. I also need a trinocular head.
My question is, is there anyone who refurbishes these scopes and can supply the needed parts to convert the scope to polarized light.
Thanks ................
Frank
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 11, 25 -- From frank_karl-at-ardl.com Thu May 26 09:21:10 2011 11, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4QEL9UF017516 11, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 26 May 2011 09:21:10 -0500 11, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 11, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 26 11, 25 -- May 2011 10:21:09 -0400 11, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 11, 25 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com} 11, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 11, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 11, 25 -- Date: Thu, 26 May 2011 10:21:07 -0400 11, 25 -- Subject: Zeiss photomicroscopeIII 11, 25 -- Thread-Topic: Zeiss photomicroscopeIII 11, 25 -- Thread-Index: AcwbsCdaAAJVCMWzRPm7p2ZK3Y64gg== 11, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077CD096A50-at-exchange2k7.ad.ardl.com} 11, 25 -- Accept-Language: en-US 11, 25 -- Content-Language: en-US 11, 25 -- X-MS-Has-Attach: 11, 25 -- X-MS-TNEF-Correlator: 11, 25 -- acceptlanguage: en-US 11, 25 -- Content-Type: text/plain; charset="iso-8859-1" 11, 25 -- MIME-Version: 1.0 11, 25 -- Content-Transfer-Encoding: 8bit 11, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4QEL9UF017516 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 36 -- From FMonson-at-wcupa.edu Thu May 26 11:04:15 2011 24, 36 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 24, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4QG4F58008008 24, 36 -- for {microscopy-at-microscopy.com} ; Thu, 26 May 2011 11:04:15 -0500 24, 36 -- X-ASG-Debug-ID: 1306425854-0378c35d273e01d0001-4CH8be 24, 36 -- Received: from WCU-XCH-02.PASSHE.LCL (wcu-xch-02.wcupa.edu [144.26.0.86]) by MX02.WCUPA.EDU with ESMTP id 12469v3JqiKbND3W (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 26 May 2011 12:04:14 -0400 (EDT) 24, 36 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 24, 36 -- X-Barracuda-Apparent-Source-IP: 144.26.0.86 24, 36 -- X-ASG-Whitelist: Client 24, 36 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 24, 36 -- WCU-XCH-02.PASSHE.LCL ([fe80::7c95:2a81:a8ef:432b%16]) with mapi id 24, 36 -- 14.01.0270.001; Thu, 26 May 2011 12:04:14 -0400 24, 36 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 24, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 24, 36 -- Subject: RE: [Microscopy] Zeiss photomicroscopeIII 24, 36 -- Thread-Topic: [Microscopy] Zeiss photomicroscopeIII 24, 36 -- X-ASG-Orig-Subj: RE: [Microscopy] Zeiss photomicroscopeIII 24, 36 -- Thread-Index: AQHMG7EydgM4BJsS3UmsLWHSFZEt2JSfPQNh 24, 36 -- Date: Thu, 26 May 2011 16:04:13 +0000 24, 36 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C0620B1-at-WCU-XCH-03.PASSHE.LCL} 24, 36 -- References: {201105261428.p4QESYu8030443-at-ns.microscopy.com} 24, 36 -- In-Reply-To: {201105261428.p4QESYu8030443-at-ns.microscopy.com} 24, 36 -- Accept-Language: en-US 24, 36 -- Content-Language: en-US 24, 36 -- X-MS-Has-Attach: 24, 36 -- X-MS-TNEF-Correlator: 24, 36 -- x-originating-ip: [10.32.20.205] 24, 36 -- Content-Type: text/plain; charset="us-ascii" 24, 36 -- MIME-Version: 1.0 24, 36 -- X-Barracuda-Connect: wcu-xch-02.wcupa.edu[144.26.0.86] 24, 36 -- X-Barracuda-Start-Time: 1306425854 24, 36 -- X-Barracuda-Encrypted: AES128-SHA 24, 36 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 24, 36 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 24, 36 -- Content-Transfer-Encoding: 8bit 24, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4QG4F58008008 ==============================End of - Headers==============================
for me, one reference paper on this topic is here:
Schroeder-Reiter E, Houben A, Wanner G (2003) Chromosoma Research 11: 585-596
FE-SEM of chromosomes, with nanogold ... have a close look.
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 8, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu May 26 12:41:53 2011 8, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4QHfq9v031729 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 26 May 2011 12:41:52 -0500 8, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 8, 25 -- by localhost (Postfix) with SMTP id B733824F0 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 26 May 2011 19:41:47 +0200 (CEST) 8, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 8, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 96408251A 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 26 May 2011 19:41:47 +0200 (CEST) 8, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 8, 25 -- with Novell_GroupWise; Thu, 26 May 2011 19:41:47 +0200 8, 25 -- Message-Id: {4DDEACFD0200005400025903-at-gwsmtp1.uni-regensburg.de} 8, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 8, 25 -- Date: Thu, 26 May 2011 19:41:49 +0200 8, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 8, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 8, 25 -- Subject: Immunogold with SEM 8, 25 -- References: {201105261236.p4QCarOx006137-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201105261236.p4QCarOx006137-at-ns.microscopy.com} 8, 25 -- Mime-Version: 1.0 8, 25 -- Content-Type: text/plain; charset=US-ASCII 8, 25 -- Content-Disposition: inline 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4QHfq9v031729 ==============================End of - Headers==============================
Hello all I have been asked to embed some paper to enable cross section viewing in the sem. I thought I could use epofix resin then microtome to leave a smooth block face. But any tips on embedding the sample to ensure I get a good cross section of the paper. I have some BEEM capsules or a flat mold available.
Many thanks NikkiThis message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.
This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 2, 34 -- From Nicola.Weston-at-nottingham.ac.uk Fri May 27 03:41:09 2011 2, 34 -- Received: from thb-mta-19.emailfiltering.com (thb-mta-19-tx.emailfiltering.com [194.116.199.150]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4R8f8Nq032026 2, 34 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 03:41:08 -0500 2, 34 -- Received: from smtp1.nottingham.ac.uk ([128.243.44.4]) 2, 34 -- by thb-mta-19.emailfiltering.com with emfmta (version 4.8.1.33) vanilla id 1972587762 2, 34 -- for microscopy-at-microscopy.com;e0d47fa035750408; Fri, 27 May 2011 09:41:07 +0100 2, 34 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 2, 34 -- by smtp1.nottingham.ac.uk with esmtp (Exim 4.60) 2, 34 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 2, 34 -- id 1QPsbT-0001gX-Q1 2, 34 -- for microscopy-at-microscopy.com; Fri, 27 May 2011 09:41:07 +0100 2, 34 -- Received: from UIWEXHUB02.ad.nottingham.ac.uk ([128.243.15.132]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 2, 34 -- Fri, 27 May 2011 09:41:07 +0100 2, 34 -- Received: from EXCHANGE1.ad.nottingham.ac.uk ([fe80::7962:f868:e6ee:6267]) by 2, 34 -- UIWEXHUB02.ad.nottingham.ac.uk ([2002:80f3:f84::80f3:f84]) with mapi; Fri, 27 2, 34 -- May 2011 09:41:07 +0100 2, 34 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 34 -- Date: Fri, 27 May 2011 09:40:50 +0100 2, 34 -- Subject: Embedding paper 2, 34 -- Thread-Topic: Embedding paper 2, 34 -- Thread-Index: AcwcScgr1xizhTxwR6qQvMRDnVcIvA== 2, 34 -- Message-ID: {443CA551C991D84AB0FF11A607D4D46C0B95EA581C-at-EXCHANGE1.ad.nottingham.ac.uk} 2, 34 -- Accept-Language: en-US, en-GB 2, 34 -- Content-Language: en-US 2, 34 -- X-MS-Has-Attach: 2, 34 -- X-MS-TNEF-Correlator: 2, 34 -- acceptlanguage: en-US, en-GB 2, 34 -- Content-Type: text/plain; charset="us-ascii" 2, 34 -- MIME-Version: 1.0 2, 34 -- X-OriginalArrivalTime: 27 May 2011 08:41:07.0810 (UTC) FILETIME=[D2956C20:01CC1C49] 2, 34 -- Content-Transfer-Encoding: 8bit 2, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4R8f8Nq032026 ==============================End of - Headers==============================
This issue has already been dealt with in the past. You may want to search the archives of the MicroscopyListserver for 2009. This message has no chance to contain viruses and infect your computer ;-)
Regards, Stephane
----- Original Message ---- X-from: "Nicola.Weston-at-nottingham.ac.uk" {Nicola.Weston-at-nottingham.ac.uk} To: nizets2-at-yahoo.com Sent: Fri, May 27, 2011 10:44:45 AM
Hello all I have been asked to embed some paper to enable cross section viewing in the sem. I thought I could use epofix resin then microtome to leave a smooth block face. But any tips on embedding the sample to ensure I get a good cross section of the paper. I have some BEEM capsules or a flat mold available.
Many thanks NikkiThis message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.
This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 2, 34 -- From Nicola.Weston-at-nottingham.ac.uk Fri May 27 03:41:09 2011 2, 34 -- Received: from thb-mta-19.emailfiltering.com (thb-mta-19-tx.emailfiltering.com [194.116.199.150]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4R8f8Nq032026 2, 34 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 03:41:08 -0500 2, 34 -- Received: from smtp1.nottingham.ac.uk ([128.243.44.4]) 2, 34 -- by thb-mta-19.emailfiltering.com with emfmta (version 4.8.1.33) vanilla id 1972587762 2, 34 -- for microscopy-at-microscopy.com;e0d47fa035750408; Fri, 27 May 2011 09:41:07 +0100 2, 34 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 2, 34 -- by smtp1.nottingham.ac.uk with esmtp (Exim 4.60) 2, 34 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 2, 34 -- id 1QPsbT-0001gX-Q1 2, 34 -- for microscopy-at-microscopy.com; Fri, 27 May 2011 09:41:07 +0100 2, 34 -- Received: from UIWEXHUB02.ad.nottingham.ac.uk ([128.243.15.132]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 2, 34 -- Fri, 27 May 2011 09:41:07 +0100 2, 34 -- Received: from EXCHANGE1.ad.nottingham.ac.uk ([fe80::7962:f868:e6ee:6267]) by 2, 34 -- UIWEXHUB02.ad.nottingham.ac.uk ([2002:80f3:f84::80f3:f84]) with mapi; Fri, 27 2, 34 -- May 2011 09:41:07 +0100 2, 34 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 34 -- Date: Fri, 27 May 2011 09:40:50 +0100 2, 34 -- Subject: Embedding paper 2, 34 -- Thread-Topic: Embedding paper 2, 34 -- Thread-Index: AcwcScgr1xizhTxwR6qQvMRDnVcIvA== 2, 34 -- Message-ID: {443CA551C991D84AB0FF11A607D4D46C0B95EA581C-at-EXCHANGE1.ad.nottingham.ac.uk} 2, 34 -- Accept-Language: en-US, en-GB 2, 34 -- Content-Language: en-US 2, 34 -- X-MS-Has-Attach: 2, 34 -- X-MS-TNEF-Correlator: 2, 34 -- acceptlanguage: en-US, en-GB 2, 34 -- Content-Type: text/plain; charset="us-ascii" 2, 34 -- MIME-Version: 1.0 2, 34 -- X-OriginalArrivalTime: 27 May 2011 08:41:07.0810 (UTC) FILETIME=[D2956C20:01CC1C49] 2, 34 -- Content-Transfer-Encoding: 8bit 2, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4R8f8Nq032026 ==============================End of - Headers==============================
==============================Original Headers============================== 16, 43 -- From nizets2-at-yahoo.com Fri May 27 04:07:34 2011 16, 43 -- Received: from nm9.bullet.mail.sp2.yahoo.com (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) 16, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4R97X7R016410 16, 43 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 04:07:33 -0500 16, 43 -- Received: from [98.139.91.64] by nm9.bullet.mail.sp2.yahoo.com with NNFMP; 27 May 2011 09:07:33 -0000 16, 43 -- Received: from [98.139.91.4] by tm4.bullet.mail.sp2.yahoo.com with NNFMP; 27 May 2011 09:07:32 -0000 16, 43 -- Received: from [127.0.0.1] by omp1004.mail.sp2.yahoo.com with NNFMP; 27 May 2011 09:07:32 -0000 16, 43 -- X-Yahoo-Newman-Property: ymail-3 16, 43 -- X-Yahoo-Newman-Id: 815279.87847.bm-at-omp1004.mail.sp2.yahoo.com 16, 43 -- Received: (qmail 65033 invoked by uid 60001); 27 May 2011 09:07:32 -0000 16, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1306487252; bh=T57c40FpUjZRdffbMYPiNH8lywUn/bpQFTQDUZ/ToLo=; h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=prG6f8jn472pYYpHfzZAYmPWo6aZgXwUMWS66vQbIizEPU6Cj9Puab+D44fo3uubs7bIDKzchFOKtmrsO/pUJZWnJgdwUrAMM66SZ4sxnDU1fnxAZ3l2hWqBFNZolt/L8xdw/2t4dIq0G4/1lh7bK8TNAFSyEzLa103pSYLV1M0= 16, 43 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 16, 43 -- s=s1024; d=yahoo.com; 16, 43 -- h=Message-ID:X-YMail-OSG:Received:X-Mailer:References:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 16, 43 -- b=Cvq2NySDZFjPTMph5f4dzs90nqiEwnT38QkVNpiH8kh/JF7WIQgubAgEEFbCPcHBnin+SdpK+YWD6VfbuXXOPkeUP414Z+9NLtH5XMxq2inechbAlUo5iRgd2KyFqaxOmYsCcPm+hhijJgEWu44WZ43rTbidIbBpQDthGGoMGrk=; 16, 43 -- Message-ID: {322090.60309.qm-at-web110811.mail.gq1.yahoo.com} 16, 43 -- X-YMail-OSG: NQLmTk8VM1nWoOf1knr49cQU9cxTDrXBzbuEL_Je.DIXmoj 16, 43 -- KI7WC53HMhyqsg.KaPq2n3Wpj0WEIYvxihA5MFxjRA_T1eBwcIB1lJatllFg 16, 43 -- cCkBzsvpSzjr_UI8IbSfS0d5T2SOwwnvjkRWTKg8Xpu1abso32TTlfQq6GTc 16, 43 -- U8cCpm749Rmk1trG7gvr.f5LwDLL.YuR5AD7.jWD40HxEsfIG8Rw4wSXaknm 16, 43 -- JJTpBsS20U8v1LQ98Iz0aAmRIsRZu4BSpJER67.xi7WaRG21ooX4CGNf7ktl 16, 43 -- dGCUK3g41ACA_4Dx.LU61hgbYYMaGdf5Q5eetTcoB9tZOqaLDUl1jWSZdL_P 16, 43 -- Euygb2O0dZVe3U44MfHU_8f0MIGDEQ5kscylQxCgA7940jQKGWs8t8ywHFzv 16, 43 -- wk3sMTPKubFvNYsUClcVX77p8H.fnFcROg6uwFpGusx_f0TchODG_Icf9RHW 16, 43 -- oSUtt8gzQQlLpVxjfQNCplNCyWwCgdSHFJy_RXwuEqoJyO4vOyLm.Az71XAK 16, 43 -- TD5wJZqGGAtTSRIhXtNgTca7AQnlabJi_NriFGcqCNVISvlQciwrowpL.Qpq 16, 43 -- MP7cgH1BIIlaxL8GdoYppH.7qum.1whQQfLs4nany5cQWcWYqTPwidaPpUkZ 16, 43 -- f.tGoTPaVyX5.bJJ._KdwgJyPLT1pPsqH0zMlmd9Ysw8ruZ7aClt6SLyVT_Z 16, 43 -- xNPxr5bazIpRx_qHklSwXDTByFc7nvOrCY_REODTbE1U7rgPtAsI0rjbYSTy 16, 43 -- SYJxVVhx.xUqBGzPyAfO3l3YaFzKYoAs3XsMNAlvpJ1AeX1aMUdW_izcGk_Q 16, 43 -- BB5q9Z4jIym84jfA- 16, 43 -- Received: from [80.122.101.100] by web110811.mail.gq1.yahoo.com via HTTP; Fri, 27 May 2011 02:07:32 PDT 16, 43 -- X-Mailer: YahooMailRC/567 YahooMailWebService/0.8.111.304355 16, 43 -- References: {201105270844.p4R8ijH0005362-at-ns.microscopy.com} 16, 43 -- Date: Fri, 27 May 2011 02:07:32 -0700 (PDT) 16, 43 -- From: Stephane Nizet {nizets2-at-yahoo.com} 16, 43 -- Subject: Re: [Microscopy] Embedding paper 16, 43 -- To: microscopy-at-microscopy.com 16, 43 -- In-Reply-To: {201105270844.p4R8ijH0005362-at-ns.microscopy.com} 16, 43 -- MIME-Version: 1.0 16, 43 -- Content-Type: text/plain; charset=iso-8859-1 16, 43 -- Content-Transfer-Encoding: 8bit 16, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p4R97X7R016410 ==============================End of - Headers==============================
VACANCY: JOHNSON MATTHEY Technology Centre - Sonning Common (approx. 35 miles West of Central London)
A research scientist position specialising in Electron Microscopy has arisen working with both Scanning and Transmission electron microscopes to characterise JM catalysts and materials.
See www.matthey.com/cgi-bin/JobView.pl?id=677&location=9 for more detailed information.
Closing date for applications: 24th June 2011
For further information contact:
Georgie Floyd, Personnel Officer, Johnson Matthey Technology Centre, Blounts Court, Sonning Common, Reading, RG4 9NH, UK or e-mail hrjmtc-at-matthey.com
Johnson Matthey Plc is an equal opportunities employer and positively encourages applications from suitably qualified and eligible candidates regardless of sex, race, disability, age, sexual orientation, religion or belief.
If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey PLC has its main place of business at 5th Floor, 25 Farringdon Street, London (020 7269 8400).
Johnson Matthey Public Limited Company Registered Office: 5th Floor, 25 Farringdon Street, London EC4A 4AB Registered in England No 33774
Whilst Johnson Matthey aims to keep its network free from viruses you should note that we are unable to scan certain emails, particularly if any part is encrypted or password-protected, and accordingly you are strongly advised to check this email and any attachments for viruses. The company shall NOT ACCEPT any liability with regard to computer viruses transferred by way of email.
Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.
==============================Original Headers============================== 9, 23 -- From goodlg-at-matthey.com Fri May 27 05:19:11 2011 9, 23 -- Received: from cluster-b.mailcontrol.com (cluster-b.mailcontrol.com [85.115.56.190]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4RAJAcI001088 9, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2011 05:19:11 -0500 9, 23 -- Received: from royhosmtp.pmp.royston.matthey.com ([193.113.135.105]) 9, 23 -- by rly37b.srv.mailcontrol.com (MailControl) with ESMTP id p4RAJ98L015132 9, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2011 11:19:09 +0100 9, 23 -- Received: from tsqhof6.trafalgar.jm ([192.168.1.238]) 9, 23 -- by royhosmtp.pmp.royston.matthey.com (8.13.6/8.13.6) with ESMTP id p4RAJ9oE010088 9, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2011 11:19:09 +0100 9, 23 -- Received: from London-MTA by tsqhof6.trafalgar.jm 9, 23 -- with Novell_GroupWise; Fri, 27 May 2011 11:19:09 +0100 9, 23 -- Message-Id: {4DDF88A50200002000008D66-at-tsqhof6.trafalgar.jm} 9, 23 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 9, 23 -- Date: Fri, 27 May 2011 11:19:01 +0100 9, 23 -- From: "Gregory Goodlet" {goodlg-at-matthey.com} 9, 23 -- To: {Microscopy-at-microscopy.com} 9, 23 -- Subject: Vacancy: TEM/SEM (UK) 9, 23 -- Mime-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=US-ASCII 9, 23 -- Content-Transfer-Encoding: 7bit 9, 23 -- Content-Disposition: inline 9, 23 -- X-Scanned-By: MailControl A-10-80-00 (www.mailcontrol.com) on 10.66.0.147 ==============================End of - Headers==============================
The determination of $50K - $60K as being a reasonable price for sustainable service of $600K instrument is based on.... 10% rule of thumb, but also 17+ years servicing various industrial and analytical SEMs and FIBs, of which 7 years as a third-party service provider. I can not speak about TEMs as I do not know that segment, but no matter how I do the numbers for FIBs or SEMs, calculation for the cost of sustainable (meaning: without pre-programmed End Of Life for the instrument) service comes close to 10% +/- .
I agree that a very large customer base, in close proximity from each other and with the same type of instruments, may allow providing sustainable service with price tag lower then the 10% "rule of thumb" - maybe. Same as small customer base with diverse instruments would push for higher price of the service.
It may seem that for very expensive instruments 10% is an overkill, until you come across just one case of replacing high-voltage parts or major column components.
I also agree that mentioned earlier annual service contract, priced at 15% of the tool cost seem to be excessive. Unless, of cause, there was something else going on behind the scenes that we do not know about. For example, it could have happened that eager-to-make-a-deal sales people agreed to give unconditional-no-matter-what-all-inclusive warranty to win the sale, but then tool was abused during first year and most of repairs were due to user damage. It is reasonable to expect that in such case service organization will try to recover the losses. Who knows?
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/26/2011 9:42 AM, john.robson-at-boehringer-ingelheim.com wrote: } Hi Valery, } } Question: Based upon what information, other than the 10% rule, have you } arrived at the conclusion that $50k - %60 is a reasonable amount? I never } mentioned which SEM I was running, the service history of the SEM / facility, } or what materials were being examined? An across the board determination } fails to consider several risks associated with each instrument. } } I totally agree that a service organization needs to turn a profit and } investors should have every expectation that they have made a wise } investment. I also agree that there is a degree of uncertainty with regards } to the liability associated with equipment failure. Costs associated with } both technician time (and travel/housing/food costs) and replacement parts is } a gamble for the service provider, although a larger customer base mitigates } the risk. In fact a case could easily be made that lower cost service } contracts with a higher customer base could lead to greater profits. } However, my comments with regards to a sweeping across the board 10% rule } actually goes to the financial risks associated with each type of instrument. } Customers with high cost instruments, with only a slightly higher cost of } maintenance, shouldn't bare the brunt of the service organizations profits. } The price of a service contract should be determined by the actual costs to } maintain the tool with a reasonable amount of profit tacked on to the bill. } This does vary by instrument type and design but doesn't track very well with } purchase price of the equipment. Suppose you're in a multi-user facility } with a track record of heavy handed use leading to frequent repairs? Perhaps } you work with materials that require frequent visits to clean up a messy } column? Either situation could, and does, result in multiple years where } service costs exceed 10%? Does the 10% rule still apply? I suppose if } you're running the lab it sounds about right! :-) } } However, going back to the original posting in this thread, it sounds like } Rosemary White's instrument isn't very reliable and her facility is being } extorted by the manufacturer. If I were in the market for a new ESEM I would } contact her off line, you might want to get the name of that company! But it } does go straight to the heart of my argument regarding "10%". Her service } provider has crunched the numbers and for her particular failure prone ESEM } they need to charge 15% to keep their organization going! } } My experience has been that service contract costs, for the high ticket } instruments (that work!), don't track with the 10% rule. And my service } company has still managed to turn a profit from my site every year for 20 } years. } } John A. Robson } } -----Original Message----- } From: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } Sent: Wednesday, May 25, 2011 5:37 PM } To: Robson,John (AN) BIP-US-R } Cc: microscopy-at-microscopy.com } Subject: Re: [Microscopy] Re: Microscope service strategies - numbers } } Hi John, } } The 10% rule applies to all-inclusive support by external organization } and must sustain existence of such service organization, cover all } costs, risks, and provide reasonable profit. The amount of $50K - $60K } per year are about right, it will be hard to sustain business of the } support organization otherwise. } } Of cause, you can do self-service and keep tools running at much lower } costs: for a while, on some instruments, and with higher risks. That is } a business decision made by many and it may be justified in your } particular situation. Just understand clearly what are the risks that } you are taking and accept the fact that whatever happens you are (or } your institution) will be on your (its) own. } } Cheers :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } } On 5/25/2011 10:49 AM, john.robson-at-boehringer-ingelheim.com wrote: } } The 10% rule would value a service contract at $50K- $60K to maintain an } SEM } } with a purchase price of $500K - $600K. That sounds somewhat unjustifiably } } high assuming your proximity to the service technician doesn't entail } } extensive travel time/costs. In my opinion the 10% formula doesn't track } } very well as the purchase price of the instrument increases from entry } level } } to "top of the line". I have maintained 2 somewhat costly FESEMs over the } } past 20+ years and in my experience their service history and cost of } } ownership don't support the 10% rule. The cost to maintain a high end } system } } is only marginally higher than a less costly entry level model yet the 10% } } rule could easily double the service contract price. } } } } } } John A. Robson } } } } -----Original Message----- } } From: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } } Sent: Tuesday, May 24, 2011 11:17 PM } } To: Robson,John (AN) BIP-US-R } } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Catching up with e-mail.... } } } } I fully agree with Ken - an all-inclusive (parts and labor) service } } contract, priced around 10% of the new instrument cost, is about right } } to keep most FIBs and SEMs running at OEM-spec level virtually } } indefinitely, but surely for 10+ years after the formally-declared EOL. } } Cost may get higher by 2% - 3% if the instrument is *very* old (20+ } } years) or if the customer with a single tool is located very remotely } } from the service organization. It is also reasonable for customer to } } expect that if FIB/SEM tool is covered by all-inclusive service } } contract, then its consumable parts should be provided at significant } } discount comparatively to the open-market prices. } } } } The obvious dilemma "do I keep spending money on servicing this tool, or } } do I run it to ground and get a new one in a couple of years" is a } } business decision which everyone makes based on his/her/institution } } financial situation. } } } } Cheers :) } } } } Valery Ray } } ================================= } } PBS&T, MEO Engineering Co., Inc. } } 290 Broadway, Suite 298 } } Methuen, MA 01844 USA } } Phone: +1-978-296-5063 } } US Mobile: +1-978-305-0479 } } Skype: pbstmeo } } E-mail: vray-at-partbeamsystech.com } } Web: www.partbeamsystech.com } } } } On 5/17/2011 9:00 AM, kenconverse-at-qualityimages.biz wrote: } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Hi Rosemary, } } } Your confocal sounds about right, although with only about a 25% increase } } } over 10 years, I think you can count your blessings. } } } } } } As for your ESEM, I should think the terms should look very much like your } } } confocal contract. Are they claiming that all the break-downs were caused } } } by mis-use and abuse? If that were the case, they shouldn't have covered } } } the repairs even under warranty. On the other hand, if the failures were } } } due to their poor design and construction, they certainly have a lot of } } } nerve implying that your contract is being increased because of that. I'm } } } also very surprised that parts are not covered. To me, that is not a } } } service contract, particularly for a new instrument. It can be } } } understandable for very old instruments, but should be reflected in the } } } pricing. } } } } } } Whoever the vendor is, I would certainly have a hard time recommending } them } } } to anyone, given the situation you describe. } } } } } } Ken Converse } } } owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } -----Original Message----- } } } X-from: rosemary.white-at-csiro.au [mailto:rosemary.white-at-csiro.au] } } } Sent: Monday, May 16, 2011 10:01 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Dear all, } } } } } } Just to get some numbers here, how much is reasonable for a service } } } contract? 10% of original cost of the instrument? 15% of cost? } } } } } } We have a service contract on one instrument (confocal) that was (10 years } } } ago) about 8% of original cost, now is about 10%. This is essentially a } } } full extended warranty, and includes an annual service visit, unlimited } } } emergency callouts and all replacement parts, even lasers are included. } } } It's been great. I don't think they have this type of contract for new } } } instruments, though(c) } } } } } } However, for a new instrument (ESEM) from a different supplier, we were } } } quoted about 15% of the original cost for a contract with 2 annual } } } maintenance visits plus unlimited emergency callouts, but no coverage of } } } replacement parts. For 2 maintenance visits and 2 emergency callouts but } } } no parts the cost went down to about 8% of initial cost. The reason given } } } for the cost was that we had many breakdowns in the 12 month warranty } } } period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, } } } computer fan blew up, etc, etc), and they had to factor this in. } } } Considering the presumed reliability of SEMs compared to confocals (in my } } } experience before this new instrument), and that no parts were covered, it } } } seemed quite expensive. } } } } } } Is the latter about what you'd expect to pay for a service contract? } } } } } } Thanks, } } } Rosemary White } } } } } } Dr Rosemary White } } } CSIRO Plant Industry } } } GPO Box 1600 } } } Canberra, ACT 2601 } } } Australia } } } } } } T 61 2 6246 5475 } } } F 61 2 6246 5334 } } } E rosemary.white-at-csiro.au } } } } } } } } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 } } } 2011 } } } 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au } } } [150.229.7.36]) } } } 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } p4H1wX9q015257 } } } 13, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 } } } -0500 } } } 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } } } 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; } } } 13, 32 -- s=email; t=1305597514; x=1337133514; } } } 13, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: } } } 13, 32 -- content-transfer-encoding; } } } 13, 32 -- bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; } } } 13, 32 -- b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv } } } 13, 32 -- VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb } } } 13, 32 -- +BsypGn+/TtdMUH; } } } 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; } } } 13, 32 -- d="scan'208";a="20957752" } } } 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) } } } 13, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May } } 2011 } } } 11:58:32 +1000 } } } 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by } } } 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Croix Snapp/AMPAC/ECCI-at-ECCI as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Kaolin Dispersant Message: I am an intern who is looking for an ideal dispersant for Kaolin clays, in order to observe individual Kaolin plates under a Jeol JSM-6700F Field Emission Scanning Electron Microscope. Would anyone on the listserver have a suggestion? At the moment I need as many ideas as possible, hopefully from a list of ionic dispersants.
Croix, This was many years ago, but at the US Geological survey where I worked for a while, various cores were dispersed with Calgon (water softener) and an ultra-sonic probe (marketed as a cell disrupter). I don't know if there are any down sides to using it with Kaolin, but it might be worth a try.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, May 27, 2011 9:49 AM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Croix Snapp/AMPAC/ECCI-at-ECCI as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Kaolin Dispersant Message: I am an intern who is looking for an ideal dispersant for Kaolin clays, in order to observe individual Kaolin plates under a Jeol JSM-6700F Field Emission Scanning Electron Microscope. Would anyone on the listserver have a suggestion? At the moment I need as many ideas as possible, hopefully from a list of ionic dispersants.
Again it appears that I haven't clearly conveyed my point. I don't disagree that 10% across the board satisfies the service companies need, and expectation, of turning a profit. My point is that an across the board 10% charge totally disregards the individual risks (instrument type, user competency, sample type, etc...) associated with each contract. A somewhat analogous example would be a homeowner near a nuclear reactor in Sendai Japan paying for insurance at the same rate as someone living near Peabody Massachusetts. Aside from the universal risks of fire (normal wear and tear on an instrument), accident (expensive but infrequent failure e.g. power supply / lens), etc... clearly the risks associated with living in Sendai... potential earthquakes (instrument type-with features known to have short lifespan), cyclone(sample type-known to require frequent cleaning), tsunami (usage - high usage multi-user facility), radioactive contamination (technical competency - inexperienced / poorly supervised users) warrants some add-on to the premium?
Another analogy would be 2 drivers with the same exact make and model car. One lives in the Bronx and works/drives to Manhattan has had 3 speeding tickets, 2 accidents, and a DWI all in the past year. The other lives in Milford Pennsylvania, works 1 mile from home and has been driving for 30 years without incident. Would they pay the same car insurance? I think not, so why would an SEM be treated any differently?
Certainly economy of scale is an issue too, manufacturers service organization vs. 3rd party providers? We also haven't mentioned response time. Immediate response is a factor one should expect to increase the cost of your premium?
FYI Truth be told Valery, immediately after sending my last reply I regretted comments I made regarding the ESEM manufacture. We only have one side of the story. Although I find it hard to believe 2 mother boards and a computer fan failure, along with multiple other failures, were caused by the operator my comments were based on insufficient "hearsay" information (No offense intended Rosemary).
Regards, John A. Robson
-----Original Message----- X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] Sent: Friday, May 27, 2011 8:26 AM To: Robson,John (AN) BIP-US-R Cc: microscopy-at-microscopy.com
1. I would suggest reviewing the following papers to get a feel for some of the issues with clay prep:
Bohor, Scanning Electron Microscopy of Clays and Clay Minerals, Clay Clay Min, 19, 1, 49-54, 1971
Sing, An Electron Optical Investigation of the Alteration of Kaolinite to Halloysite, Clays Clay Min, 40, 2, 212-229, 1992
Zbik, Nanomorphology of Kaolinites, Comparative SEM and AFM Studies, Clays Clay Min, 46, 2, 153-160, 1998
Keller, Scanning Electron Micrographs of Kaolins Collected from Diverse Environments of Origin-I, Clays Clay Min, 24, 3, 107-113, 1976
Escudey, Apparent Dissolution During Ultrasonic Dispersion of Allophanic Soils and Soil Fractions, Clays Clay Min, 37, 5, 493-496, 1989
Jernigan, Critical Point Drying of Electron Microscope Samples of Clay Minerals, Clay Clay Min, 23, 2, 161-162, 1975
2. Also, I should mention that Debbie Sherman at Purdue has fair amount of work with clays and could probably give more ideas but since there is no direct email to reply on this post, I'll let her respond if she chooses.
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123 www.ph2llc.com (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
Tony
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, May 27, 2011 9:50 AM To: ph2-at-sprynet.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Croix Snapp/AMPAC/ECCI-at-ECCI as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Kaolin Dispersant Message: I am an intern who is looking for an ideal dispersant for Kaolin clays, in order to observe individual Kaolin plates under a Jeol JSM-6700F Field Emission Scanning Electron Microscope. Would anyone on the listserver have a suggestion? At the moment I need as many ideas as possible, hopefully from a list of ionic dispersants.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/27/2011 4:59 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both Angel.Paredes-at-fda.hhs.gov as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Angel.Paredes-at-fda.hhs.gov Name: Angel Paredes } } Organization: NCTR } } Title-Subject: [Filtered] Carbon evaporator } } Message: Hi, } } I am in need of a used carbon evaporator. Does anyone know we could get } relatively quickly while we wait for the new one we ordered to arrive? } } Sincerely, } Angel Paredes } NCTR } Jefferson, AR 72079 } } Login Host: 150.148.0.65 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri May 27 15:58:09 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4RKw9Jw011620 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 15:58:09 -0500 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 52FF23A24D9 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 15:58:09 -0500 (CDT) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id Jo8xZpYD2YcT for {microscopy-at-microscopy.com} ; } 10, 25 -- Fri, 27 May 2011 15:58:08 -0500 (CDT) } 10, 25 -- Received: from NestorPwrBk.local (unknown [220.118.58.41]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id 7942E3A24CE } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 15:58:08 -0500 (CDT) } 10, 25 -- Message-ID: {4DE0105B.2050004-at-microscopy.com} } 10, 25 -- Date: Sat, 28 May 2011 05:58:03 +0900 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: Angel.Paredes-at-fda.hhs.gov } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: viaWWW:Carbon evaporator } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 43 -- From vray-at-partbeamsystech.com Fri May 27 16:58:58 2011 5, 43 -- Received: from nm11.bullet.mail.sp2.yahoo.com (nm11.bullet.mail.sp2.yahoo.com [98.139.91.81]) 5, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4RLww4W028561 5, 43 -- for {microscopy-at-microscopy.com} ; Fri, 27 May 2011 16:58:58 -0500 5, 43 -- Received: from [98.139.91.70] by nm11.bullet.mail.sp2.yahoo.com with NNFMP; 27 May 2011 21:58:58 -0000 5, 43 -- Received: from [98.139.91.48] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 27 May 2011 21:58:58 -0000 5, 43 -- Received: from [127.0.0.1] by omp1048.mail.sp2.yahoo.com with NNFMP; 27 May 2011 21:58:58 -0000 5, 43 -- X-Yahoo-Newman-Id: 26956.55053.bm-at-omp1048.mail.sp2.yahoo.com 5, 43 -- Received: (qmail 58625 invoked from network); 27 May 2011 21:58:57 -0000 5, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1306533537; bh=BaYRcn7hmu6rOshMfLXO9Nix31k7aOF+aRSQoDeDu2k=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=PD6V3svgL3GPkyWtHrOe9Nf+xOhUlCZn0Y4iDy3cJ0et4CSm0KVEXjZkTO9eUBcQpq/5osyMLGt6sE+CFIiqWInm72xhHQJa6F/jMfLZXQt+qjXf2FgGXMyeDQbtAiJE8PAaYKPE78jwplhPIN/YmY++yG10dyGKKJcDm193ISM= 5, 43 -- Received: from [192.168.2.31] (vray-at-75.68.231.25 with plain) 5, 43 -- by smtp115.biz.mail.sp1.yahoo.com with SMTP; 27 May 2011 14:58:57 -0700 PDT 5, 43 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 43 -- X-YMail-OSG: gzVrXqoVM1klS0VIx9Bdx93zzUXoZ76wROc1hOl3RzSDLJJ 5, 43 -- _47hkh_I8XSCCj34U8DScBL3jv9aJYWc7LPPLbjX1OYqk.v_DI0YMTkYsFQx 5, 43 -- yghXoLIZvcqsqgZpzrc_ykAPTvUX_ykc.SuynwozNcGkL._Ds9Ej50q8XgR8 5, 43 -- cg1ricM5fMA7YpfEs.U0ma91YYCScKph_mYb8HF2SGR8sY33shbJmgmOMMcS 5, 43 -- Dxi__Wz.ydzizaf.nH_hyPwepfuhqXLQbUjczVrDX2XEGNY_JGWsiFMoHooC 5, 43 -- lM.4GpJ8wRN6FeHHSYUSHDwebfmPwoAr8hm0OvyL5FNm9mYjL_Bw42P5ehJR 5, 43 -- 8LTDFxSFkh1gGTceA00YmKQwp7TNDROZPXY4dIAwu0E9Zx3Rhd_lv.RLmvsG 5, 43 -- B3v3jWD6SJZDH4a3SxAn752GeHrnxMFf04mbhzKw22YrsKjGsbLIBLkb6HEU 5, 43 -- YfzZeulnaEzK4hogORWcDiZ9aryW5KBeXe5K139LEFHYnhqXZ8.CZrmGc7SR 5, 43 -- 0YSSwMRArSEYrMgPM_VD6MGXkzqvvkvPblJnkthNlRPK4gnrQYLbTCwoHzGi 5, 43 -- XTuUZARemXlwh2PZOkFSMK2RI8tRRr6EDCJ8Z6M428pytQxGOc07eemmBlIR 5, 43 -- RahAekhdF3twCgfWsdebSeKo8mkfFHGPTadAB3QXIrqdurqUuC1MGStrIb9p 5, 43 -- vhfwP1hHaFivjqIiyZ6RBwwewzBN2xQZHtON7gBxwkKt8Lygs3OgThpRPwdS 5, 43 -- V5izbBbzXT.hU5W74j7WqdTefPumIDBpsUVPOr0iHiZ9qk9WVh3rVHXCeCIM 5, 43 -- QaMyAC5DkqilTGdeUP6sqJynuKlSSIZbKqi7055_Ofoc8GBDURkUNp0K95bI 5, 43 -- tLeCI1rby4ULgoIdDnp6uYfTzKw-- 5, 43 -- X-Yahoo-Newman-Property: ymail-3 5, 43 -- Message-ID: {4DE01EA2.60702-at-partbeamsystech.com} 5, 43 -- Date: Fri, 27 May 2011 17:58:58 -0400 5, 43 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 43 -- Reply-To: vray-at-partbeamsystech.com 5, 43 -- Organization: PBS&T 5, 43 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 5, 43 -- MIME-Version: 1.0 5, 43 -- To: microscopy-at-microscopy.com, Angel.Paredes-at-fda.hhs.gov 5, 43 -- Subject: Re: [Microscopy] viaWWW:Carbon evaporator 5, 43 -- References: {201105272059.p4RKx9de013031-at-ns.microscopy.com} 5, 43 -- In-Reply-To: {201105272059.p4RKx9de013031-at-ns.microscopy.com} 5, 43 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 43 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Sure, of cause, specific level of coverage provided by the service contract would affect the price!
It is exactly like insurance - reduced coverage lets you reduce premium with higher risk, and higher level of coverage increases premium but can virtually eliminate risk. One can also go without insurance and bear all the risks, or anything in between - this is a business decision and the right answer is different for different users.
And yes, "15 minute call" (to alternative service provider) can save you a little bit, but be beware of "significant" savings - usually you get (quality and coverage) that you are paying for. IMHO 10% is a good reference point for full-coverage service contract on particle beam instrument. Of cause - depending on particularities, actual price may vary slightly, but in my (still limited) experience not by much...
Have a great weekend :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/27/2011 10:12 AM, john.robson-at-boehringer-ingelheim.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Valery, } } Again it appears that I haven't clearly conveyed my point. I don't disagree } that 10% across the board satisfies the service companies need, and } expectation, of turning a profit. My point is that an across the board 10% } charge totally disregards the individual risks (instrument type, user } competency, sample type, etc...) associated with each contract. A somewhat } analogous example would be a homeowner near a nuclear reactor in Sendai Japan } paying for insurance at the same rate as someone living near Peabody } Massachusetts. Aside from the universal risks of fire (normal wear and tear } on an instrument), accident (expensive but infrequent failure e.g. power } supply / lens), etc... clearly the risks associated with living in Sendai... } potential earthquakes (instrument type-with features known to have short } lifespan), cyclone(sample type-known to require frequent cleaning), tsunami } (usage - high usage multi-user facility), radioactive contamination } (technical competency - inexperienced / poorly supervised users) warrants } some add-on to the premium? } } Another analogy would be 2 drivers with the same exact make and model car. } One lives in the Bronx and works/drives to Manhattan has had 3 speeding } tickets, 2 accidents, and a DWI all in the past year. The other lives in } Milford Pennsylvania, works 1 mile from home and has been driving for 30 } years without incident. Would they pay the same car insurance? I think not, } so why would an SEM be treated any differently? } } Certainly economy of scale is an issue too, manufacturers service } organization vs. 3rd party providers? We also haven't mentioned response } time. Immediate response is a factor one should expect to increase the cost } of your premium? } } FYI Truth be told Valery, immediately after sending my last reply I regretted } comments I made regarding the ESEM manufacture. We only have one side of the } story. Although I find it hard to believe 2 mother boards and a computer fan } failure, along with multiple other failures, were caused by the operator my } comments were based on insufficient "hearsay" information (No offense } intended Rosemary). } } Regards, } John A. Robson } } -----Original Message----- } X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } Sent: Friday, May 27, 2011 8:26 AM } To: Robson,John (AN) BIP-US-R } Cc: microscopy-at-microscopy.com } Subject: Re: [Microscopy] Re: Microscope service strategies - numbers } } Hi John, } } The determination of $50K - $60K as being a reasonable price for } sustainable service of $600K instrument is based on.... 10% rule of } thumb, but also 17+ years servicing various industrial and analytical } SEMs and FIBs, of which 7 years as a third-party service provider. I can } not speak about TEMs as I do not know that segment, but no matter how I } do the numbers for FIBs or SEMs, calculation for the cost of sustainable } (meaning: without pre-programmed End Of Life for the instrument) service } comes close to 10% +/- . } } I agree that a very large customer base, in close proximity from each } other and with the same type of instruments, may allow providing } sustainable service with price tag lower then the 10% "rule of thumb" - } maybe. Same as small customer base with diverse instruments would push } for higher price of the service. } } It may seem that for very expensive instruments 10% is an overkill, } until you come across just one case of replacing high-voltage parts or } major column components. } } I also agree that mentioned earlier annual service contract, priced at } 15% of the tool cost seem to be excessive. Unless, of cause, there was } something else going on behind the scenes that we do not know about. For } example, it could have happened that eager-to-make-a-deal sales people } agreed to give unconditional-no-matter-what-all-inclusive warranty to } win the sale, but then tool was abused during first year and most of } repairs were due to user damage. It is reasonable to expect that in such } case service organization will try to recover the losses. Who knows? } } Cheers :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } } On 5/26/2011 9:42 AM, john.robson-at-boehringer-ingelheim.com wrote: } } Hi Valery, } } } } Question: Based upon what information, other than the 10% rule, have you } } arrived at the conclusion that $50k - %60 is a reasonable amount? I never } } mentioned which SEM I was running, the service history of the SEM / } facility, } } or what materials were being examined? An across the board determination } } fails to consider several risks associated with each instrument. } } } } I totally agree that a service organization needs to turn a profit and } } investors should have every expectation that they have made a wise } } investment. I also agree that there is a degree of uncertainty with } regards } } to the liability associated with equipment failure. Costs associated with } } both technician time (and travel/housing/food costs) and replacement parts } is } } a gamble for the service provider, although a larger customer base } mitigates } } the risk. In fact a case could easily be made that lower cost service } } contracts with a higher customer base could lead to greater profits. } } However, my comments with regards to a sweeping across the board 10% rule } } actually goes to the financial risks associated with each type of } instrument. } } Customers with high cost instruments, with only a slightly higher cost of } } maintenance, shouldn't bare the brunt of the service organizations profits. } } The price of a service contract should be determined by the actual costs to } } maintain the tool with a reasonable amount of profit tacked on to the bill. } } This does vary by instrument type and design but doesn't track very well } with } } purchase price of the equipment. Suppose you're in a multi-user facility } } with a track record of heavy handed use leading to frequent repairs? } Perhaps } } you work with materials that require frequent visits to clean up a messy } } column? Either situation could, and does, result in multiple years where } } service costs exceed 10%? Does the 10% rule still apply? I suppose if } } you're running the lab it sounds about right! :-) } } } } However, going back to the original posting in this thread, it sounds like } } Rosemary White's instrument isn't very reliable and her facility is being } } extorted by the manufacturer. If I were in the market for a new ESEM I } would } } contact her off line, you might want to get the name of that company! But } it } } does go straight to the heart of my argument regarding "10%". Her service } } provider has crunched the numbers and for her particular failure prone ESEM } } they need to charge 15% to keep their organization going! } } } } My experience has been that service contract costs, for the high ticket } } instruments (that work!), don't track with the 10% rule. And my service } } company has still managed to turn a profit from my site every year for 20 } } years. } } } } John A. Robson } } } } -----Original Message----- } } From: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } } Sent: Wednesday, May 25, 2011 5:37 PM } } To: Robson,John (AN) BIP-US-R } } Cc: microscopy-at-microscopy.com } } Subject: Re: [Microscopy] Re: Microscope service strategies - numbers } } } } Hi John, } } } } The 10% rule applies to all-inclusive support by external organization } } and must sustain existence of such service organization, cover all } } costs, risks, and provide reasonable profit. The amount of $50K - $60K } } per year are about right, it will be hard to sustain business of the } } support organization otherwise. } } } } Of cause, you can do self-service and keep tools running at much lower } } costs: for a while, on some instruments, and with higher risks. That is } } a business decision made by many and it may be justified in your } } particular situation. Just understand clearly what are the risks that } } you are taking and accept the fact that whatever happens you are (or } } your institution) will be on your (its) own. } } } } Cheers :) } } } } Valery Ray } } ================================= } } PBS&T, MEO Engineering Co., Inc. } } 290 Broadway, Suite 298 } } Methuen, MA 01844 USA } } Phone: +1-978-296-5063 } } US Mobile: +1-978-305-0479 } } Skype: pbstmeo } } E-mail: vray-at-partbeamsystech.com } } Web: www.partbeamsystech.com } } } } On 5/25/2011 10:49 AM, john.robson-at-boehringer-ingelheim.com wrote: } } } The 10% rule would value a service contract at $50K- $60K to maintain an } } SEM } } } with a purchase price of $500K - $600K. That sounds somewhat } unjustifiably } } } high assuming your proximity to the service technician doesn't entail } } } extensive travel time/costs. In my opinion the 10% formula doesn't track } } } very well as the purchase price of the instrument increases from entry } } level } } } to "top of the line". I have maintained 2 somewhat costly FESEMs over the } } } past 20+ years and in my experience their service history and cost of } } } ownership don't support the 10% rule. The cost to maintain a high end } } system } } } is only marginally higher than a less costly entry level model yet the 10% } } } rule could easily double the service contract price. } } } } } } } } } John A. Robson } } } } } } -----Original Message----- } } } From: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } } } Sent: Tuesday, May 24, 2011 11:17 PM } } } To: Robson,John (AN) BIP-US-R } } } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Catching up with e-mail.... } } } } } } I fully agree with Ken - an all-inclusive (parts and labor) service } } } contract, priced around 10% of the new instrument cost, is about right } } } to keep most FIBs and SEMs running at OEM-spec level virtually } } } indefinitely, but surely for 10+ years after the formally-declared EOL. } } } Cost may get higher by 2% - 3% if the instrument is *very* old (20+ } } } years) or if the customer with a single tool is located very remotely } } } from the service organization. It is also reasonable for customer to } } } expect that if FIB/SEM tool is covered by all-inclusive service } } } contract, then its consumable parts should be provided at significant } } } discount comparatively to the open-market prices. } } } } } } The obvious dilemma "do I keep spending money on servicing this tool, or } } } do I run it to ground and get a new one in a couple of years" is a } } } business decision which everyone makes based on his/her/institution } } } financial situation. } } } } } } Cheers :) } } } } } } Valery Ray } } } ================================= } } } PBS&T, MEO Engineering Co., Inc. } } } 290 Broadway, Suite 298 } } } Methuen, MA 01844 USA } } } Phone: +1-978-296-5063 } } } US Mobile: +1-978-305-0479 } } } Skype: pbstmeo } } } E-mail: vray-at-partbeamsystech.com } } } Web: www.partbeamsystech.com } } } } } } On 5/17/2011 9:00 AM, kenconverse-at-qualityimages.biz wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } } } } ---------------------------------------------------------------------------- } } } } } } } } Hi Rosemary, } } } } Your confocal sounds about right, although with only about a 25% increase } } } } over 10 years, I think you can count your blessings. } } } } } } } } As for your ESEM, I should think the terms should look very much like } your } } } } confocal contract. Are they claiming that all the break-downs were } caused } } } } by mis-use and abuse? If that were the case, they shouldn't have covered } } } } the repairs even under warranty. On the other hand, if the failures were } } } } due to their poor design and construction, they certainly have a lot of } } } } nerve implying that your contract is being increased because of that. } I'm } } } } also very surprised that parts are not covered. To me, that is not a } } } } service contract, particularly for a new instrument. It can be } } } } understandable for very old instruments, but should be reflected in the } } } } pricing. } } } } } } } } Whoever the vendor is, I would certainly have a hard time recommending } } them } } } } to anyone, given the situation you describe. } } } } } } } } Ken Converse } } } } owner } } } } } } } } QUALITY IMAGES } } } } Servicing Scanning Electron Microscopes } } } } Since 1981 } } } } 474 So. Bridgton Rd. } } } } Bridgton, ME 04009 } } } } 207-647-4348 } } } } kenconverse-at-qualityimages.biz } } } } qualityimages.biz } } } } } } } } } } } } -----Original Message----- } } } } X-from: rosemary.white-at-csiro.au [mailto:rosemary.white-at-csiro.au] } } } } Sent: Monday, May 16, 2011 10:01 PM } } } } To: kenconverse-at-qualityimages.biz } } } } Subject: [Microscopy] Re: Microscope service strategies - numbers } } } } } } } } } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } } } } } ---------------------------------------------------------------------------- } } } } } } } } Dear all, } } } } } } } } Just to get some numbers here, how much is reasonable for a service } } } } contract? 10% of original cost of the instrument? 15% of cost? } } } } } } } } We have a service contract on one instrument (confocal) that was (10 } years } } } } ago) about 8% of original cost, now is about 10%. This is essentially a } } } } full extended warranty, and includes an annual service visit, unlimited } } } } emergency callouts and all replacement parts, even lasers are included. } } } } It's been great. I don't think they have this type of contract for new } } } } instruments, though(c) } } } } } } } } However, for a new instrument (ESEM) from a different supplier, we were } } } } quoted about 15% of the original cost for a contract with 2 annual } } } } maintenance visits plus unlimited emergency callouts, but no coverage of } } } } replacement parts. For 2 maintenance visits and 2 emergency callouts but } } } } no parts the cost went down to about 8% of initial cost. The reason } given } } } } for the cost was that we had many breakdowns in the 12 month warranty } } } } period (2 mother boards, 3 different Peltier stages, 2 BSE detectors, } } } } computer fan blew up, etc, etc), and they had to factor this in. } } } } Considering the presumed reliability of SEMs compared to confocals (in my } } } } experience before this new instrument), and that no parts were covered, } it } } } } seemed quite expensive. } } } } } } } } Is the latter about what you'd expect to pay for a service contract? } } } } } } } } Thanks, } } } } Rosemary White } } } } } } } } Dr Rosemary White } } } } CSIRO Plant Industry } } } } GPO Box 1600 } } } } Canberra, ACT 2601 } } } } Australia } } } } } } } } T 61 2 6246 5475 } } } } F 61 2 6246 5334 } } } } E rosemary.white-at-csiro.au } } } } } } } } } } } } } } } } } } } } } } } } } } } } ==============================Original } } } Headers============================== } } } } 13, 32 -- From prvs=111c25117=Rosemary.White-at-csiro.au Mon May 16 20:58:34 } } } } 2011 } } } } 13, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au } } } } [150.229.7.36]) } } } } 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } } p4H1wX9q015257 } } } } 13, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 16 May 2011 20:58:33 } } } } -0500 } } } } 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } } } } 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; } } } } 13, 32 -- s=email; t=1305597514; x=1337133514; } } } } 13, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: } } } } 13, 32 -- content-transfer-encoding; } } } } 13, 32 -- bh=lQS+GFg5Kg0e5UmZOYa/zW1OZI1YQjfIXY74O3zgm1o=; } } } } 13, 32 -- b=e9k6BD2gso69GRaS/kLEosc9wrIgXI4oJOgcpiCYmztF8NiR9Dh0JQUv } } } } 13, 32 -- VKRb9mwqMAIs07TfVMQgBXA8sPAuAJHM6NpGDi+ggGjZMr0IebNMMdIyb } } } } 13, 32 -- +BsypGn+/TtdMUH; } } } } 13, 32 -- X-IronPort-AV: E=Sophos;i="4.65,222,1304258400"; } } } } 13, 32 -- d="scan'208";a="20957752" } } } } 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) } } } } 13, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 17 May } } } 2011 } } } } 11:58:32 +1000 } } } } 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by } } } } 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft
After installation, we noticed erratic EPSE signals, the EPSE detector was replaced 6 months later, but the cool stage still interfered with the detector. This went on for a further 6 months with two replacements until it was finally fixed, sort of. Even though it's marketed as a full ESEM able to be used with water vapour, it doesn't like this with the cool stage below about 3 degrees, which makes it much less useful than we anticipated. The EPSE detector finally worked correctly 14 months after installation.
The two motherboards failed two months after installation by the engineers, and it took two months before they were replaced, which seemed rather a long time.
The computer fan blew up when the engineer came to do a routine service - he was at the instrument, not one of us. Fortunately, we could just go to a local shop and get a new fan.
Weirdly, one quadrant of the BSE detector also failed during a service visit. It took 4 months to replace the BSE detector. 5 months after this, the BSE diode and pre-amp failed, and it took 6 weeks for the parts to arrive. During installation of the replacement BSE diode and pre-amp by the service engineer, the SE detector died. We had to pay the $5,000 to get it replaced.
The final straw, which I didn't mention earlier, was that we were guaranteed, in writing, that our cryostage would fit onto the new SEM, and it doesn't. The company hasn't even tried to help us with this.
When I asked about a maintenance contract, the branch manager specifically pointed out that because we'd been so expensive to them in the warranty period, our contract was more expensive and would not cover parts. She also said that the installation and warranty agreements had been heavily discounted to get sales and although our instrument was more expensive than their discounted budget, it did fall under her quote for standard install and warranty, so it's on their heads, in my mind, that it cost more than they bargained for.
Unlimited callouts plus two maintenance visits would be just under 15% of the initial cost, whereas two maintenance visits plus two callouts per year would be about 7% of initial cost, and two maintenance visits with no callouts would be 3% of the initial cost. None of these cover parts.
Anyway, I'll never purchase anything from this lot again. I get annoyed with the "blame the customer" attitude. I've been in this game 25 years, and I've never had this experience before. As I mentioned, we have a confocal - in my books a much more temperamental and failure-prone beast than an SEM - and we've had a full extended warranty on it for 10 years and the company has never complained that we're too expensive. We also have a 30-year old SEM which we still use although its mechanical parts are wearing out, but it just keeps on chugging on, we've had very few problems with it.
But thanks for all the comments and numbers, it's been very useful.
Rosemary
Dr Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 28/05/11 12:18 AM, "john.robson-at-boehringer-ingelheim.com" {john.robson-at-boehringer-ingelheim.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 18, 31 -- From prvs=122d272a8=Rosemary.White-at-csiro.au Fri May 27 19:03:14 2011 18, 31 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) 18, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4S03C6f009115 18, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2011 19:03:13 -0500 18, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 18, 31 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 18, 31 -- s=email; t=1306540993; x=1338076993; 18, 31 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 18, 31 -- content-transfer-encoding; 18, 31 -- bh=rO3fEEy8Y0ze55cWWuMAT4peZLle+oAn3qmdXVzGedY=; 18, 31 -- b=QntOPY1A4aFAGHbjmspZfNSjOrCC9/PGLhnK8ooO5Dz5jPlueqc5khv0 18, 31 -- jKOiY+P8LDTt8xa2Eo+a76uKnrtIPCZtLEqWaC8ncVtBT+qvRp0QA2yfj 18, 31 -- od59/vGHlQnetWh; 18, 31 -- X-IronPort-AV: E=Sophos;i="4.65,283,1304258400"; 18, 31 -- d="scan'208";a="21747991" 18, 31 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 18, 31 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 28 May 2011 10:03:02 +1000 18, 31 -- Received: from [152.83.195.117] (152.83.195.117) by 18, 31 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 18, 31 -- (TLS) id 8.3.159.2; Sat, 28 May 2011 10:03:02 +1000 18, 31 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 18, 31 -- Date: Sat, 28 May 2011 10:02:58 +1000 18, 31 -- Subject: Re: [Microscopy] Microscope service strategies - numbers 18, 31 -- From: Rosemary White {rosemary.white-at-csiro.au} 18, 31 -- To: {Microscopy-at-microscopy.com} 18, 31 -- Message-ID: {CA066CBD.19F7B%rosemary.white-at-csiro.au} 18, 31 -- Thread-Topic: [Microscopy] Microscope service strategies - numbers 18, 31 -- In-Reply-To: {201105271418.p4REIRxf004191-at-ns.microscopy.com} 18, 31 -- MIME-Version: 1.0 18, 31 -- Content-Type: text/plain; charset="US-ASCII" 18, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
1. Based on your recollection of the comments from branch manager, what happened is very close to what I've guessed: eager-to-make-a-deal sales organization of the OEM discounted installation and warranty to the point that it became impossible for the service organization of the same company to profitably carry these activities. Apparently OEM does not have internal mechanism for absorbing such losses and "bailing out" local branch when and if this happens. So local branch manager tried to recover losses by passing exuberant service fees to the customer (since now you are stuck with a brand-new SEM which most of alternative support vendors would not touch). If this happened by accident then you should receive due apologies and some kind of compensation. Otherwise would mean that this was a practical execution of that OEM's business strategy...
Did you try to ask your manager about possibility of writing a letter to the CEO of that OEM company? Upward flow of negative information in large organizations is very restricted, so chances are that on the level of management where service and sales organizations of that OEM converge your story is unheard of, and people with sufficient authority to make things right are completely unaware of it! Within the OEM organization, local branch manager and sales account manager that you customary deal with, have loads of fiscal responsibilities but zero authority for resolving this kind of situations...
2. If you ever purchased a used car, you should know that salesmen would give you written guarantees for virtually anything, as long as they think that you are not likely to sue them over it and confident that your distant uncle Corleone will not ask cousin Luigi to enforce these promises. Some of SEM sales people do come from that used-car background and consider such tactics a fair game. When you get written promises, you as user should tie a *significant* portion of payment for the tool to that magic moment when promises will materialize. Or make sure that promises are on the purchase and sale agreement, do not sign for installation and do not pay for the tool until your cryo stage fits. If you already paid for the tool, then there are no meaningful money tied to the promise to fit your cryo stage. Without *significant* money tied to the promise, chances are that nobody will do anything about it. Your only possible recourse now would be to convince management of your institution to take a legal action, but over such a small (in respect of money involved) matter it probably would not make sense.
On the service side:
You had typical service-call type of support, instead of warranty coverage. Length of the lead times for parts suggests that you were sold a tool for which OEM service organization does not have parts in stock, or (even worse) have no ability to procure and deliver the parts in a timely manner. If so - what will they do in 2 years from now, when these motherboards will be out of production???
On the technical side:
One can try to argue that failure of detectors could have somehow been caused by user's mode of operation, but if they failed while service engineer was working on the tool then even this argument becomes too weak. Everything else should be pure warranty repairs... Unless of cause, there were traces of Pepsi-Cola spills on all the failed motherboards and inside of BSE preamplifier, which I doubt.
On the service cost side:
Without knowing specific details of the coverage contract, proximity of other customers, etc...: 3% for two maintenance visits, without parts and without service calls may be reasonable, 7% for two maintenance visits and two service calls starts to get high, but may still be OK if you are very remote and/or if response time is relatively short, and/or if you got unlimited phone support to help you with self-maintenance. But 15% for unlimited service calls without parts seems to be very, very steep (unless you are getting our own, dedicated, and fully-trained service engineer supporting just your tool and located 100% on-site).
The bottom line - based on the available information, I would agree with your conclusion to not buy anything from these guys. Unless, of cause, they deliver due apologies for unfortunate mistakes and misunderstandings, extend warranty for the whole period of time that it took them to stabilize your instrument and make it fully functional, and agree to provide good support at reasonable price afterward...
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com
On 5/27/2011 8:04 PM, rosemary.white-at-csiro.au wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I can give you the details and you can judge. } } After installation, we noticed erratic EPSE signals, the EPSE detector was } replaced 6 months later, but the cool stage still interfered with the } detector. This went on for a further 6 months with two replacements until } it was finally fixed, sort of. Even though it's marketed as a full ESEM } able to be used with water vapour, it doesn't like this with the cool } stage below about 3 degrees, which makes it much less useful than we } anticipated. The EPSE detector finally worked correctly 14 months after } installation. } } The two motherboards failed two months after installation by the } engineers, and it took two months before they were replaced, which seemed } rather a long time. } } The computer fan blew up when the engineer came to do a routine service - } he was at the instrument, not one of us. Fortunately, we could just go to } a local shop and get a new fan. } } Weirdly, one quadrant of the BSE detector also failed during a service } visit. It took 4 months to replace the BSE detector. 5 months after } this, the BSE diode and pre-amp failed, and it took 6 weeks for the parts } to arrive. During installation of the replacement BSE diode and pre-amp } by the service engineer, the SE detector died. We had to pay the $5,000 } to get it replaced. } } The final straw, which I didn't mention earlier, was that we were } guaranteed, in writing, that our cryostage would fit onto the new SEM, and } it doesn't. The company hasn't even tried to help us with this. } } When I asked about a maintenance contract, the branch manager specifically } pointed out that because we'd been so expensive to them in the warranty } period, our contract was more expensive and would not cover parts. She } also said that the installation and warranty agreements had been heavily } discounted to get sales and although our instrument was more expensive } than their discounted budget, it did fall under her quote for standard } install and warranty, so it's on their heads, in my mind, that it cost } more than they bargained for. } } Unlimited callouts plus two maintenance visits would be just under 15% of } the initial cost, whereas two maintenance visits plus two callouts per } year would be about 7% of initial cost, and two maintenance visits with no } callouts would be 3% of the initial cost. None of these cover parts. } } Anyway, I'll never purchase anything from this lot again. I get annoyed } with the "blame the customer" attitude. I've been in this game 25 years, } and I've never had this experience before. As I mentioned, we have a } confocal - in my books a much more temperamental and failure-prone beast } than an SEM - and we've had a full extended warranty on it for 10 years } and the company has never complained that we're too expensive. We also } have a 30-year old SEM which we still use although its mechanical parts } are wearing out, but it just keeps on chugging on, we've had very few } problems with it. } } But thanks for all the comments and numbers, it's been very useful. } } Rosemary } } Dr Rosemary White } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } E rosemary.white-at-csiro.au } } } On 28/05/11 12:18 AM, "john.robson-at-boehringer-ingelheim.com" } {john.robson-at-boehringer-ingelheim.com} wrote: } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } -------------------------------------------------------------------------- } } -- } } } } Hi Valery, } } } } Again it appears that I haven't clearly conveyed my point. I don't } } disagree } } that 10% across the board satisfies the service companies need, and } } expectation, of turning a profit. My point is that an across the board } } 10% } } charge totally disregards the individual risks (instrument type, user } } competency, sample type, etc...) associated with each contract. A somewhat } } analogous example would be a homeowner near a nuclear reactor in Sendai } } Japan } } paying for insurance at the same rate as someone living near Peabody } } Massachusetts. Aside from the universal risks of fire (normal wear and } } tear } } on an instrument), accident (expensive but infrequent failure e.g. power } } supply / lens), etc... clearly the risks associated with living in } } Sendai... } } potential earthquakes (instrument type-with features known to have short } } lifespan), cyclone(sample type-known to require frequent cleaning), } } tsunami } } (usage - high usage multi-user facility), radioactive contamination } } (technical competency - inexperienced / poorly supervised users) warrants } } some add-on to the premium? } } } } Another analogy would be 2 drivers with the same exact make and model car. } } One lives in the Bronx and works/drives to Manhattan has had 3 speeding } } tickets, 2 accidents, and a DWI all in the past year. The other lives in } } Milford Pennsylvania, works 1 mile from home and has been driving for 30 } } years without incident. Would they pay the same car insurance? I think } } not, } } so why would an SEM be treated any differently? } } } } Certainly economy of scale is an issue too, manufacturers service } } organization vs. 3rd party providers? We also haven't mentioned response } } time. Immediate response is a factor one should expect to increase the } } cost } } of your premium? } } } } FYI Truth be told Valery, immediately after sending my last reply I } } regretted } } comments I made regarding the ESEM manufacture. We only have one side of } } the } } story. Although I find it hard to believe 2 mother boards and a computer } } fan } } failure, along with multiple other failures, were caused by the operator } } my } } comments were based on insufficient "hearsay" information (No offense } } intended Rosemary). } } } } Regards, } } John A. Robson } } } } -----Original Message----- } } X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } } Sent: Friday, May 27, 2011 8:26 AM } } To: Robson,John (AN) BIP-US-R } } Cc: microscopy-at-microscopy.com } } Subject: Re: [Microscopy] Re: Microscope service strategies - numbers } } } } Hi John, } } } } The determination of $50K - $60K as being a reasonable price for } } sustainable service of $600K instrument is based on.... 10% rule of } } thumb, but also 17+ years servicing various industrial and analytical } } SEMs and FIBs, of which 7 years as a third-party service provider. I can } } not speak about TEMs as I do not know that segment, but no matter how I } } do the numbers for FIBs or SEMs, calculation for the cost of sustainable } } (meaning: without pre-programmed End Of Life for the instrument) service } } comes close to 10% +/- . } } } } I agree that a very large customer base, in close proximity from each } } other and with the same type of instruments, may allow providing } } sustainable service with price tag lower then the 10% "rule of thumb" - } } maybe. Same as small customer base with diverse instruments would push } } for higher price of the service. } } } } It may seem that for very expensive instruments 10% is an overkill, } } until you come across just one case of replacing high-voltage parts or } } major column components. } } } } I also agree that mentioned earlier annual service contract, priced at } } 15% of the tool cost seem to be excessive. Unless, of cause, there was } } something else going on behind the scenes that we do not know about. For } } example, it could have happened that eager-to-make-a-deal sales people } } agreed to give unconditional-no-matter-what-all-inclusive warranty to } } win the sale, but then tool was abused during first year and most of } } repairs were due to user damage. It is reasonable to expect that in such } } case service organization will try to recover the losses. Who knows? } } } } Cheers :) } } } } Valery Ray } } ================================= } } PBS&T, MEO Engineering Co., Inc. } } 290 Broadway, Suite 298 } } Methuen, MA 01844 USA } } Phone: +1-978-296-5063 } } US Mobile: +1-978-305-0479 } } Skype: pbstmeo } } E-mail: vray-at-partbeamsystech.com } } Web: www.partbeamsystech.com } } } } } } } } } } ==============================Original Headers============================== } 18, 31 -- From prvs=122d272a8=Rosemary.White-at-csiro.au Fri May 27 19:03:14 2011 } 18, 31 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) } 18, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p4S03C6f009115 } 18, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 27 May 2011 19:03:13 -0500 } 18, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 18, 31 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; } 18, 31 -- s=email; t=1306540993; x=1338076993; } 18, 31 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: } 18, 31 -- content-transfer-encoding; } 18, 31 -- bh=rO3fEEy8Y0ze55cWWuMAT4peZLle+oAn3qmdXVzGedY=; } 18, 31 -- b=QntOPY1A4aFAGHbjmspZfNSjOrCC9/PGLhnK8ooO5Dz5jPlueqc5khv0 } 18, 31 -- jKOiY+P8LDTt8xa2Eo+a76uKnrtIPCZtLEqWaC8ncVtBT+qvRp0QA2yfj } 18, 31 -- od59/vGHlQnetWh; } 18, 31 -- X-IronPort-AV: E=Sophos;i="4.65,283,1304258400"; } 18, 31 -- d="scan'208";a="21747991" } 18, 31 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) } 18, 31 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 28 May 2011 10:03:02 +1000 } 18, 31 -- Received: from [152.83.195.117] (152.83.195.117) by } 18, 31 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server } 18, 31 -- (TLS) id 8.3.159.2; Sat, 28 May 2011 10:03:02 +1000 } 18, 31 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 } 18, 31 -- Date: Sat, 28 May 2011 10:02:58 +1000 } 18, 31 -- Subject: Re: [Microscopy] Microscope service strategies - numbers } 18, 31 -- From: Rosemary White {rosemary.white-at-csiro.au} } 18, 31 -- To: {Microscopy-at-microscopy.com} } 18, 31 -- Message-ID: {CA066CBD.19F7B%rosemary.white-at-csiro.au} } 18, 31 -- Thread-Topic: [Microscopy] Microscope service strategies - numbers } 18, 31 -- In-Reply-To: {201105271418.p4REIRxf004191-at-ns.microscopy.com} } 18, 31 -- MIME-Version: 1.0 } 18, 31 -- Content-Type: text/plain; charset="US-ASCII" } 18, 31 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 15, 43 -- From vray-at-partbeamsystech.com Sat May 28 10:42:03 2011 15, 43 -- Received: from nm15.bullet.mail.ac4.yahoo.com (nm15.bullet.mail.ac4.yahoo.com [98.139.52.212]) 15, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p4SFg2gI015568 15, 43 -- for {microscopy-at-microscopy.com} ; Sat, 28 May 2011 10:42:03 -0500 15, 43 -- Received: from [98.139.52.189] by nm15.bullet.mail.ac4.yahoo.com with NNFMP; 28 May 2011 15:42:02 -0000 15, 43 -- Received: from [98.139.52.134] by tm2.bullet.mail.ac4.yahoo.com with NNFMP; 28 May 2011 15:42:02 -0000 15, 43 -- Received: from [127.0.0.1] by omp1017.mail.ac4.yahoo.com with NNFMP; 28 May 2011 15:42:02 -0000 15, 43 -- X-Yahoo-Newman-Id: 503903.61956.bm-at-omp1017.mail.ac4.yahoo.com 15, 43 -- Received: (qmail 6686 invoked from network); 28 May 2011 15:42:02 -0000 15, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1306597322; bh=TfmLZbZ9YBPQq5vDbLGOaJ7o10mw0u4qVWYfQA/EFvY=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=O2uE0ZuWpV/2rLHt83ixL/krrRezttbyLJJ40Z6E1JBtUHNFrGhMav2JT8I+lzSbePihiiGhd/W9vK9s2RXNISBURSn0Q5AHLmFa2UYIuQFhlWvXI/Ar1BehUFOwWcd6gM/3NnuZMskWYFwhbKVyXHiLXjhyZ8p9cCX1BL6W69w= 15, 43 -- Received: from [192.168.2.31] (vray-at-75.68.231.25 with plain) 15, 43 -- by smtp111.biz.mail.mud.yahoo.com with SMTP; 28 May 2011 08:42:01 -0700 PDT 15, 43 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 15, 43 -- X-YMail-OSG: fPHfpX8VM1lipG93jwiBVblA7Ni.kZorBfi3i09pj29Mrna 15, 43 -- HXIAamp_KteVk3sCeGizUani58S9YeTe1RzGrgctLW.keUHzMKv3l5MMUvQB 15, 43 -- H3XCrSEITgGJ_phVARWH_jTiav1tNDfl6HFZC5HPzoxtmvQoYEldVtla8anO 15, 43 -- _GzLcTP5rdz45h681MlMyZlFi86reV5duTDtkxb8VPeruMJWxXHnt049mnHu 15, 43 -- BPgWMNyNyvK9cUs42cOwWbbUpeJSquHQlo7TTKiiPmOcie7tMTgaRk6laj1F 15, 43 -- 0iYxUwigdlPVxvUAkxTqUyqGBtvG_LGb3HMtsqHPlYOBUOWdvFAnYZnzV4hp 15, 43 -- 1ov5IiBRcQAwltNah18HLieILROFrlS1TecENQKwQlYQMY2u7bVXggOEyQ_F 15, 43 -- aNNuTdBMnw1ltjxw6h4ODsuByxeYrXj6AjPMb66Enc1TAd2mo6H9ZRkCavT5 15, 43 -- V_w5BZAAQtPmE2q0P__4WfRceuqV2rRgvEG9VZ2vTUlCO9IRbk7aYI5_icu0 15, 43 -- 0jRXIA30LY_1e_i0jmVqRPDIeyigkoLUQg8tFwkeReUngTF7z9e7LN6H5b3W 15, 43 -- osT26XOfgFtUTpVuC_cpaXbux_ocb_Y7c8QEiYc0g_h_dtMvXZ9NHu_3r7ay 15, 43 -- sh7p.rW8pB0uGDCeZLDkizYW8QYkmNR_YWieA6z3DEX2yiP0pODO.JiUm5Q8 15, 43 -- QOJAFhNSsXrnxgYaqtwvFebIcpCGKIsqSEnRGbXieAHVC999I8U8Zb66OIQV 15, 43 -- AABbU4e9YUftitXOHnKP1iH_f8IrtSGD8ekSHG1yxwyXdgBk.epmlpw6B1Wt 15, 43 -- nEP9r_w.teCBOPZJbKfiPxL1TMmM4rQ06rfLgNeR9hLZAF.vrArs_xqQU5vN 15, 43 -- i5Q8uTd4bVXBCf73. 15, 43 -- X-Yahoo-Newman-Property: ymail-3 15, 43 -- Message-ID: {4DE117C8.1060204-at-partbeamsystech.com} 15, 43 -- Date: Sat, 28 May 2011 11:42:00 -0400 15, 43 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 15, 43 -- Reply-To: vray-at-partbeamsystech.com 15, 43 -- Organization: PBS&T 15, 43 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 15, 43 -- MIME-Version: 1.0 15, 43 -- To: rosemary.white-at-csiro.au, microscopy-at-microscopy.com 15, 43 -- Subject: Re: [Microscopy] Re: Microscope service strategies - numbers 15, 43 -- References: {201105280004.p4S04B0J010643-at-ns.microscopy.com} 15, 43 -- In-Reply-To: {201105280004.p4S04B0J010643-at-ns.microscopy.com} 15, 43 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 43 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both petra.rosner-at-ww.uni-erlangen.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: petra.rosner-at-ww.uni-erlangen.de Name: Petra Rosner
Organization: Universität Erlangen
Title-Subject: [Filtered] LR White
Message: Hello does anybody know thomething about the shrinking of LR White. What kind of embedding material for ultramicrotomy ( we want to cut Ag nanoparitcles) is the one with the least shrinking? thanks for all suggestions Petra
The University of Texas Southwestern Medical Center at Dallas has an immediate opening for an electron microscopy technician. The lab is a core facility serving the medical campus. We have a well-equipped lab and will be moving to a purpose built new facility at the beginning of 2012. The candidate should ideally have experience in some or all of : Resin sample preparation and sectioning TEM operation and ability to train others Immunolabeling using cryo preparation methods and/or high pressure freezing, freeze substitution Good communication and customer service skills A willingness to work to tight deadlines and attention to detail Digital imaging, computer and networking skills
Further information about the lab can be found at http://www4.utsouthwestern.edu/mcif/index.htm
Please contact me for more details or to arrange a preliminary phone interview.
Our institutions policy on diversity can be found at http://www.utsouthwestern.edu/utsw/cda/dept36801/files/269061.html
Christopher J Gilpin Ph.D. Assistant Professor Director, Molecular and Cellular Imaging Facility K1.A04 UT Southwestern Medical Center at Dallas 5323 Harry Hines Boulevard Dallas, TX 75390-9039 Phone +1 214 648 2827
________________________________
UT Southwestern Medical Center The future of medicine, today.
==============================Original Headers============================== 11, 37 -- From christopher.gilpin-at-utsouthwestern.edu Thu Jun 2 13:51:55 2011 11, 37 -- Received: from zixvpm02.utsouthwestern.edu (zixvpm02.utsouthwestern.edu [199.242.239.76]) 11, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p52Iptcl008624 11, 37 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jun 2011 13:51:55 -0500 11, 37 -- Received: from zixvpm02.utsouthwestern.edu (ZixVPM [127.0.0.1]) 11, 37 -- by Outbound.utsouthwestern.edu (Proprietary) with ESMTP id D906C4C14B 11, 37 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jun 2011 13:51:54 -0500 (CDT) 11, 37 -- Received: from swlxsmtp2.swmed.edu (swlxsmtp2.swmed.edu [199.165.152.167]) 11, 37 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 11, 37 -- (No client certificate requested) 11, 37 -- by zixvpm02.utsouthwestern.edu (Proprietary) with ESMTP id 7AEEC4C1B0 11, 37 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jun 2011 13:51:41 -0500 (CDT) 11, 37 -- Received: from swmshub1.swmed.org ([199.242.234.130]) 11, 37 -- by swlxsmtp2.swmed.edu with esmtp (Exim 4.71) 11, 37 -- (envelope-from {Christopher.Gilpin-at-UTSouthwestern.edu} ) 11, 37 -- id 1QSCzd-0003Mf-Ak 11, 37 -- for microscopy-at-microscopy.com; Thu, 02 Jun 2011 13:51:41 -0500 11, 37 -- Received: from swmsmail4.swmed.org ([199.242.234.123]) by swmshub1 11, 37 -- ([199.242.234.116]) with mapi id 14.01.0289.001; Thu, 2 Jun 2011 13:51:40 11, 37 -- -0500 11, 37 -- From: Christopher Gilpin {Christopher.Gilpin-at-utsouthwestern.edu} 11, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 37 -- Thread-Topic: Open position for an EM technician 11, 37 -- Thread-Index: AcwhVJiq/JOSSVwZRUyUjgPiXjxFog== 11, 37 -- Date: Thu, 2 Jun 2011 18:51:40 +0000 11, 37 -- Message-ID: {D827A312AC5735449076C42E1A5157DC04D1E37B-at-swmsmail4.swmed.org} 11, 37 -- Accept-Language: en-US 11, 37 -- Content-Language: en-US 11, 37 -- X-MS-Has-Attach: 11, 37 -- X-MS-TNEF-Correlator: 11, 37 -- x-originating-ip: [129.112.115.6] 11, 37 -- Content-Type: text/plain; charset="iso-8859-1" 11, 37 -- MIME-Version: 1.0 11, 37 -- X-Scan-Signature: 40cf72356374e0af06fce51ab8fa263b 11, 37 -- Subject: Open position for an EM technician 11, 37 -- Content-Transfer-Encoding: 8bit 11, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p52Iptcl008624 ==============================End of - Headers==============================
For those running cooled CCD cameras on your TEM's, do you have a problem with them acting like cold traps, with the surface of the scintillator becoming contaminated?
Thanks, Randy
________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________
==============================Original Headers============================== 7, 27 -- From randy-nessler-at-uiowa.edu Thu Jun 2 13:57:50 2011 7, 27 -- Received: from HC-EDGE2.healthcare.uiowa.edu (hc-edge2.healthcare.uiowa.edu [129.255.126.23]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p52IvoG7016300 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Jun 2011 13:57:50 -0500 7, 27 -- Received: from HC-HUB2.healthcare.uiowa.edu (129.255.112.195) by 7, 27 -- HC-EDGE2.healthcare.uiowa.edu (129.255.126.23) with Microsoft SMTP Server 7, 27 -- (TLS) id 14.1.289.1; Thu, 2 Jun 2011 13:57:46 -0500 7, 27 -- Received: from HC-MAILBOXC1-N5.healthcare.uiowa.edu ([169.254.5.3]) by 7, 27 -- HC-HUB2.healthcare.uiowa.edu ([129.255.112.195]) with mapi id 14.01.0289.001; 7, 27 -- Thu, 2 Jun 2011 13:57:49 -0500 7, 27 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 7, 27 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 7, 27 -- Subject: Contamination on TEM CCD camera 7, 27 -- Thread-Topic: Contamination on TEM CCD camera 7, 27 -- Thread-Index: AcwhVqVuyhlCbU0rSi+6imTxcQICQg== 7, 27 -- Date: Thu, 2 Jun 2011 18:57:49 +0000 7, 27 -- Message-ID: {962DA0541BCBF346AAC391AE682C87A2048EC5EC-at-HC-MAILBOXC1-N5.healthcare.uiowa.edu} 7, 27 -- Accept-Language: en-US 7, 27 -- Content-Language: en-US 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- x-originating-ip: [172.26.0.1] 7, 27 -- x-my-disclaimer: ready 7, 27 -- Content-Type: text/plain; charset="us-ascii" 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p52IvoG7016300 ==============================End of - Headers==============================
Of course! That's why there is usually a control to turn off the Peltier when the camera is not in use, or even a heater to outgas it periodically. How much you need to use these controls, though, depends on how clean you vacuum is.
A. John Mardinly, ASU
-----Original Message----- X-from: randy-nessler-at-uiowa.edu [mailto:randy-nessler-at-uiowa.edu] Sent: Thursday, June 02, 2011 12:05 PM To: John Mardinly
For those running cooled CCD cameras on your TEM's, do you have a problem with them acting like cold traps, with the surface of the scintillator becoming contaminated?
Thanks, Randy
________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________
==============================Original Headers============================== 7, 27 -- From randy-nessler-at-uiowa.edu Thu Jun 2 13:57:50 2011 7, 27 -- Received: from HC-EDGE2.healthcare.uiowa.edu (hc-edge2.healthcare.uiowa.edu [129.255.126.23]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p52IvoG7016300 7, 27 -- for {Microscopy-at-Microscopy.Com} ; Thu, 2 Jun 2011 13:57:50 -0500 7, 27 -- Received: from HC-HUB2.healthcare.uiowa.edu (129.255.112.195) by 7, 27 -- HC-EDGE2.healthcare.uiowa.edu (129.255.126.23) with Microsoft SMTP Server 7, 27 -- (TLS) id 14.1.289.1; Thu, 2 Jun 2011 13:57:46 -0500 7, 27 -- Received: from HC-MAILBOXC1-N5.healthcare.uiowa.edu ([169.254.5.3]) by 7, 27 -- HC-HUB2.healthcare.uiowa.edu ([129.255.112.195]) with mapi id 14.01.0289.001; 7, 27 -- Thu, 2 Jun 2011 13:57:49 -0500 7, 27 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 7, 27 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 7, 27 -- Subject: Contamination on TEM CCD camera 7, 27 -- Thread-Topic: Contamination on TEM CCD camera 7, 27 -- Thread-Index: AcwhVqVuyhlCbU0rSi+6imTxcQICQg== 7, 27 -- Date: Thu, 2 Jun 2011 18:57:49 +0000 7, 27 -- Message-ID: {962DA0541BCBF346AAC391AE682C87A2048EC5EC-at-HC-MAILBOXC1-N5.healthcare.uio wa.edu} 7, 27 -- Accept-Language: en-US 7, 27 -- Content-Language: en-US 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- x-originating-ip: [172.26.0.1] 7, 27 -- x-my-disclaimer: ready 7, 27 -- Content-Type: text/plain; charset="us-ascii" 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p52IvoG7016300 ==============================End of - Headers==============================
==============================Original Headers============================== 15, 40 -- From John.Mardinly-at-asu.edu Thu Jun 2 15:37:03 2011 15, 40 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p52Kb3tF010021 15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 2 Jun 2011 15:37:03 -0500 15, 40 -- X-ASG-Debug-ID: 1307047022-032fb5780001-4CH8be 15, 40 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet8.asu.edu with ESMTP id M4xZUQjcnqhJztZF (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Thu, 02 Jun 2011 13:37:02 -0700 (MST) 15, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 15, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 15, 40 -- X-ASG-Whitelist: Client 15, 40 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.196) by 15, 40 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 15, 40 -- 8.1.436.0; Thu, 2 Jun 2011 13:37:01 -0700 15, 40 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by 15, 40 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 2 Jun 15, 40 -- 2011 13:37:01 -0700 15, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 15, 40 -- Content-Class: urn:content-classes:message 15, 40 -- MIME-Version: 1.0 15, 40 -- Content-Type: text/plain; charset="US-ASCII" 15, 40 -- Subject: RE: [Microscopy] Contamination on TEM CCD camera 15, 40 -- Date: Thu, 2 Jun 2011 13:37:01 -0700 15, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] Contamination on TEM CCD camera 15, 40 -- Message-ID: {95FFD84122D92D4F913DE75095E9C42E0661041D-at-EX05.asurite.ad.asu.edu} 15, 40 -- In-Reply-To: {201106021904.p52J4Val007930-at-ns.microscopy.com} 15, 40 -- X-MS-Has-Attach: 15, 40 -- X-MS-TNEF-Correlator: 15, 40 -- Thread-Topic: [Microscopy] Contamination on TEM CCD camera 15, 40 -- Thread-Index: AcwhV+sgSak3cZ3GQMG6ZQ38gXcykgADKnHg 15, 40 -- References: {201106021904.p52J4Val007930-at-ns.microscopy.com} 15, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 15, 40 -- To: {randy-nessler-at-uiowa.edu} 15, 40 -- CC: {microscopy-at-microscopy.com} 15, 40 -- X-OriginalArrivalTime: 02 Jun 2011 20:37:01.0144 (UTC) FILETIME=[D33E8D80:01CC2164] 15, 40 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 15, 40 -- X-Barracuda-Start-Time: 1307047022 15, 40 -- X-Barracuda-Encrypted: RC4-MD5 15, 40 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 15, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 15, 40 -- Content-Transfer-Encoding: 8bit 15, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p52Kb3tF010021 ==============================End of - Headers==============================
And what if the temperature that the heat cycle obtains does not cause sublimation of the contaminants?
I've had a couple of off-line replies that are curious about this as well. Thanks, Randy
-----Original Message----- X-from: John Mardinly [mailto:John.Mardinly-at-asu.edu] Sent: Thursday, June 02, 2011 3:37 PM To: Nessler, Randy A Cc: microscopy-at-microscopy.com
If the oil gets decomposed by the beam, it will never come off and you may need a new phosphor. The worse I have seen was a very old camera that was oil coupled from the phosphor to the fiber optic-the oil leaked out and got on the phosphor.
A. John Mardinly, ASU
-----Original Message----- X-from: Nessler, Randy A [mailto:randy-nessler-at-uiowa.edu] Sent: Thursday, June 02, 2011 1:40 PM To: John Mardinly Cc: microscopy-at-microscopy.com
Hello Listers,
I just received an e-mail from our TEM service engineer stating that Santovac5 is a controlled substance that must be kept secure and can't be sold or transferred to foreign nationals, even if they are located in the USA.
Here is what he sent me:
"Santovac-5 can be used as a space optimized lubricant (Specifically it is used on Nuclear Launch Vehicles). It is controlled by the Bureau of Industry and Security. It carries an ECCN # of 1C006. Small quantities are allowed to be exported assuming they fall under a value threshold. If the shipment goes above this a license is required. While this isn’t an export, transfer to a foreign national is an export even when conducted inside the US borders. It may seem silly but it’s 100K per violation so it is serious.
Do not allow access to the item by any foreign national. Also never leave it alone unsecured, it is to be on your person, or locked in a secure location until it is used."
I'm sure lots of labs have Santovac just sitting out on shelves.
Has anyone heard of this? If so, can you explain it so I understand the reasoning? It's not super clear to me.
Have a great weekend!
Paula :-) -- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 10, 33 -- From patpxs-at-gmail.com Fri Jun 3 06:08:10 2011 10, 33 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53B89SI005902 10, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 06:08:09 -0500 10, 33 -- Received: by iwg8 with SMTP id 8so2040464iwg.0 10, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 03 Jun 2011 04:08:08 -0700 (PDT) 10, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 33 -- d=gmail.com; s=gamma; 10, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 10, 33 -- :content-type:content-transfer-encoding; 10, 33 -- bh=nX8VkX8LYr5cTGf3EFOFVbZIYqsysVcJtvX+IHejj30=; 10, 33 -- b=byPYD+uodz9g9tdHNoo8e27kGltswUaOzlbbHp0DEK79Bp+j/PMMZvQQdWYywWy9ez 10, 33 -- tEfWk95hzMs9He2hcP+0rGCLUZGsHCeO5oun5CCdLVeUm4/467OabfmnW8y4dz3+A9qj 10, 33 -- c8h0qjnFA6mRZ7X7kPv+34zKWClqgsxhjOps4= 10, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 10, 33 -- d=gmail.com; s=gamma; 10, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 10, 33 -- :content-transfer-encoding; 10, 33 -- b=wd+dItLuLGv2/nMDSMpaxQ/rAyciH/bL9rEjQiIzrF2DlMrNGV5yVajhjXTY0TiP5k 10, 33 -- R2uperc0d+n4UDEo4Igtjg51Dnz4mvLq1AqfC318Yf8XzwuhlosFj7Om9SuIEdFl/gb5 10, 33 -- 7QqCJk8ZFXILzstegjYES0F6gegXbaNOtt1wc= 10, 33 -- MIME-Version: 1.0 10, 33 -- Received: by 10.42.137.193 with SMTP id z1mr2827233ict.455.1307099288639; Fri, 10, 33 -- 03 Jun 2011 04:08:08 -0700 (PDT) 10, 33 -- Received: by 10.42.222.138 with HTTP; Fri, 3 Jun 2011 04:08:08 -0700 (PDT) 10, 33 -- Date: Fri, 3 Jun 2011 07:08:08 -0400 10, 33 -- Message-ID: {BANLkTi=CMsh2ut1PPFiQYY8fW4AMQQxWkg-at-mail.gmail.com} 10, 33 -- Subject: Santovac5 10, 33 -- From: Paula Sicurello {patpxs-at-gmail.com} 10, 33 -- To: Microscopy-at-microscopy.com 10, 33 -- Content-Type: text/plain; charset=windows-1252 10, 33 -- Content-Transfer-Encoding: 8bit 10, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p53B89SI005902 ==============================End of - Headers==============================
I found that Santovac5 isn't one of the items but a bunch of hydrocarbons are: The fed's list the CAS's as 25497-30-7, 124-73-2, and 27336-23-8. Santovac is 2455-71-2. Even if it is covered the regulations are talking about 55 gallon barrels.
Seems like someone at FEI should get their Fedreal Regulations straight. Not that they aren't a moving target.
It appears that humanity is still safe from the dreaded and expensive Santovac5.
Paula
Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Fri, Jun 3, 2011 at 8:35 AM, Cammer, Michael {Michael.Cammer-at-med.nyu.edu} wrote: } And is there any research lab in the US that isn't staffed by foreign nationals? } } -----Original Message----- } From: patpxs-at-gmail.com [mailto:patpxs-at-gmail.com] } Sent: Friday, June 03, 2011 7:20 AM } To: Cammer, Michael } Subject: [Microscopy] Santovac5 } } } Do not allow access to the item by any foreign national. } Also never leave it alone unsecured, it is to be on your person, or } locked in a secure location until it is used." } } } } ------------------------------------------------------------ } This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. } ================================= } }
--
==============================Original Headers============================== 10, 36 -- From patpxs-at-gmail.com Fri Jun 3 07:51:38 2011 10, 36 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53Cpb8w023692 10, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 07:51:37 -0500 10, 36 -- Received: by iyl8 with SMTP id 8so714510iyl.0 10, 36 -- for {Microscopy-at-microscopy.com} ; Fri, 03 Jun 2011 05:51:37 -0700 (PDT) 10, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 36 -- d=gmail.com; s=gamma; 10, 36 -- h=domainkey-signature:mime-version:in-reply-to:references:date 10, 36 -- :message-id:subject:from:to:content-type:content-transfer-encoding; 10, 36 -- bh=Fbfi/s3ezc9KdsjT81+OARC2okPQbc4xa24RNZguXP8=; 10, 36 -- b=R+n6jlfujbKcQ/mj1vH9xju68vykTsyph9PTDHS/6pa44W/08Y1d4HRcW7Crw7e+yG 10, 36 -- qs5LmBJ/7rTcS4hG7K4HWSHc6WbFRofMCY0lfKXjnnzUGrxCelYeqMrBoL36HBR8j8q7 10, 36 -- MYyc0MFPNCup9RF0F8m1v4SR/yhJ/2GU/DNCM= 10, 36 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 10, 36 -- d=gmail.com; s=gamma; 10, 36 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 10, 36 -- :content-type:content-transfer-encoding; 10, 36 -- b=vBhAYbmVnfVdKsisYGFGWCW563E8mbDArLYZZhbhh9P76iE2CWeLnIm72kKapDduyP 10, 36 -- 2RPTGcroKSfbMI+0KpVECW6tiAiKGaW5SmQ3lMXgJ/jRBXLO5RhIf3tZUwFOpTyVaPJy 10, 36 -- 5ETBwTWxq4qifZwLWtqIbmchAXWXwNwjzohKc= 10, 36 -- MIME-Version: 1.0 10, 36 -- Received: by 10.42.163.130 with SMTP id c2mr3002273icy.522.1307105496937; Fri, 10, 36 -- 03 Jun 2011 05:51:36 -0700 (PDT) 10, 36 -- Received: by 10.42.222.138 with HTTP; Fri, 3 Jun 2011 05:51:36 -0700 (PDT) 10, 36 -- In-Reply-To: {725986F6C4F57449A27E7D10B49F82B33A019CFDC4-at-MSGWSDCPMB06.nyumc.org} 10, 36 -- References: {201106031120.p53BK7rc020457-at-ns.microscopy.com} 10, 36 -- {725986F6C4F57449A27E7D10B49F82B33A019CFDC4-at-MSGWSDCPMB06.nyumc.org} 10, 36 -- Date: Fri, 3 Jun 2011 08:51:36 -0400 10, 36 -- Message-ID: {BANLkTim_rwh-DWPoRpBnmqArmS4BA134RQ-at-mail.gmail.com} 10, 36 -- Subject: Re: [Microscopy] Santovac5 10, 36 -- From: Paula Sicurello {patpxs-at-gmail.com} 10, 36 -- To: "Cammer, Michael" {Michael.Cammer-at-med.nyu.edu} , Microscopy-at-microscopy.com 10, 36 -- Content-Type: text/plain; charset=ISO-8859-1 10, 36 -- Content-Transfer-Encoding: 8bit 10, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p53Cpb8w023692 ==============================End of - Headers==============================
Rats. There go my dreams of getting rich smuggling Santovac into Canada ...
Phil
} I dug through the federal regulations, whew! } } I found that Santovac5 isn't one of the items but a bunch of } hydrocarbons are: The fed's list the CAS's as 25497-30-7, 124-73-2, } and 27336-23-8. Santovac is 2455-71-2. Even if it is covered the } regulations are talking about 55 gallon barrels. } } Seems like someone at FEI should get their Fedreal Regulations } straight. Not that they aren't a moving target. } } It appears that humanity is still safe from the dreaded and expensive } Santovac5. } } Paula } } Paula Sicurello, HTL (ASCP) } Supervisor, Electron Microscope Laboratory } Duke University Health System } Rm.#251M, Duke South, Green Zone } Durham, North Carolina 27710 } P: 919.684.2091 } On Fri, Jun 3, 2011 at 8:35 AM, Cammer, Michael } {Michael.Cammer-at-med.nyu.edu} wrote: } } And is there any research lab in the US that isn't staffed by } } foreign nationals? } } } } -----Original Message----- } } From: patpxs-at-gmail.com [mailto:patpxs-at-gmail.com] } } Sent: Friday, June 03, 2011 7:20 AM } } To: Cammer, Michael } } Subject: [Microscopy] Santovac5 } } } } } } Do not allow access to the item by any foreign national. } } Also never leave it alone unsecured, it is to be on your person, or } } locked in a secure location until it is used."
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 4, 29 -- From oshel1pe-at-cmich.edu Fri Jun 3 08:00:22 2011 4, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53D0M0L005048 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 08:00:22 -0500 4, 29 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 4, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p53D0GGr031156 4, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 09:00:21 -0400 4, 29 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 4, 29 -- Fri, 3 Jun 2011 08:59:44 -0400 4, 29 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 29 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Fri, 3 Jun 4, 29 -- 2011 08:59:44 -0400 4, 29 -- MIME-Version: 1.0 4, 29 -- Message-ID: {a06240802ca0e8b084502-at-[141.209.160.249]} 4, 29 -- In-Reply-To: {201106031254.p53CsptF028799-at-ns.microscopy.com} 4, 29 -- References: {201106031254.p53CsptF028799-at-ns.microscopy.com} 4, 29 -- Date: Fri, 3 Jun 2011 08:59:41 -0400 4, 29 -- To: {Microscopy-at-microscopy.com} 4, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 29 -- Subject: [Microscopy] Re: Santovac5 4, 29 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 29 -- X-Originating-IP: [141.209.160.249] 4, 29 -- X-OriginalArrivalTime: 03 Jun 2011 12:59:44.0194 (UTC) FILETIME=[1BF5FA20:01CC21EE] 4, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 29 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 4, 29 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 29 -- X-CanItPRO-Stream: default 4, 29 -- X-Canit-Stats-ID: 02EPp0lql - dfe981af4fd2 - 20110603 4, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Geez Phil First it's Santovac, next you'll graduate to Apiezon L, or, help us, N. Will it stop before the bottom, before Vestopal W or (psst, really I have some legitimate) Epon 812....
I'm gettin' worried phil ;).
paul -- Paul R. Hazelton, PhD Gastroenteric Diseases Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 730 William Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca Phone: 204-789-3313 (w) 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 21 -- From paul_hazelton-at-umanitoba.ca Fri Jun 3 09:30:25 2011 5, 21 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53EUPAu025131 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 09:30:25 -0500 5, 21 -- Received: from [140.193.25.116] (basic116.medmb.umanitoba.ca [140.193.25.116]) 5, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) 5, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p53EUISe005461 5, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); 5, 21 -- Fri, 3 Jun 2011 09:30:18 -0500 (CDT) 5, 21 -- Message-ID: {4DE8EFFC.8030209-at-umanitoba.ca} 5, 21 -- Date: Fri, 03 Jun 2011 09:30:20 -0500 5, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} 5, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 5, 21 -- MIME-Version: 1.0 5, 21 -- To: oshel1pe-at-cmich.edu 5, 21 -- Subject: Re: [Microscopy] Re: Santovac5 5, 21 -- References: {201106031301.p53D1WLB009690-at-ns.microscopy.com} 5, 21 -- In-Reply-To: {201106031301.p53D1WLB009690-at-ns.microscopy.com} 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 21 -- Content-Transfer-Encoding: 7bit 5, 21 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
I have kept my Metallputz under lock and key with my spools of gold bonding wire, for years! 8^)
Darrell
paul_hazelton-at-umanitoba.ca wrote on 06/03/2011 10:31:27 AM:
} } [Microscopy] Re: Re: Santovac5 } } paul_hazelton } } to: } } Darrell Miles } } 06/03/2011 10:32 AM } } Please respond to paul_hazelton } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Geez Phil First it's Santovac, next you'll graduate to Apiezon L, or, } help us, N. Will it stop before the bottom, before Vestopal W or (psst,
} really I have some legitimate) Epon 812.... } } I'm gettin' worried phil ;). } } } paul } -- } Paul R. Hazelton, PhD } Gastroenteric Diseases Study Group } University of Manitoba } Department of Medical Microbiology } 511 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 3J7 } e-mail: paul_hazelton-at-umanitoba.ca } Phone: 204-789-3313 (w) } 204-489-6924 (h) } Cell: 204-781-6982 } Fax: 204-789-3926 } } } ==============================Original Headers============================== } 5, 21 -- From paul_hazelton-at-umanitoba.ca Fri Jun 3 09:30:25 2011 } 5, 21 -- Received: from electra.cc.umanitoba.ca } (electra.cc.umanitoba.ca [130.179.16.34]) } 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id p53EUPAu025131 } 5, 21 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 09:30:25 -0500 } 5, 21 -- Received: from [140.193.25.116] } (basic116.medmb.umanitoba.ca [140.193.25.116]) } 5, 21 -- (user=hazeltn mech=CRAM-MD5 bits=0) } 5, 21 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id } p53EUISe005461 } 5, 21 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO); } 5, 21 -- Fri, 3 Jun 2011 09:30:18 -0500 (CDT) } 5, 21 -- Message-ID: {4DE8EFFC.8030209-at-umanitoba.ca} } 5, 21 -- Date: Fri, 03 Jun 2011 09:30:20 -0500 } 5, 21 -- From: Paul Hazelton {paul_hazelton-at-umanitoba.ca} } 5, 21 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; } rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 } 5, 21 -- MIME-Version: 1.0 } 5, 21 -- To: oshel1pe-at-cmich.edu } 5, 21 -- Subject: Re: [Microscopy] Re: Santovac5 } 5, 21 -- References: {201106031301.p53D1WLB009690-at-ns.microscopy.com} } 5, 21 -- In-Reply-To: {201106031301.p53D1WLB009690-at-ns.microscopy.com} } 5, 21 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 5, 21 -- Content-Transfer-Encoding: 7bit } 5, 21 -- X-DCC-UofM-Metrics: electra; whitelist } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 31 -- From milesd-at-us.ibm.com Fri Jun 3 09:57:19 2011 8, 31 -- Received: from e6.ny.us.ibm.com (e6.ny.us.ibm.com [32.97.182.146]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53EvIFM009216 8, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Jun 2011 09:57:18 -0500 8, 31 -- Received: from d01relay02.pok.ibm.com (d01relay02.pok.ibm.com [9.56.227.234]) 8, 31 -- by e6.ny.us.ibm.com (8.14.4/8.13.1) with ESMTP id p53EX7Sb020741 8, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Jun 2011 10:33:07 -0400 8, 31 -- Received: from d01av03.pok.ibm.com (d01av03.pok.ibm.com [9.56.224.217]) 8, 31 -- by d01relay02.pok.ibm.com (8.13.8/8.13.8/NCO v10.0) with ESMTP id p53EuXMr118386 8, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Jun 2011 10:56:37 -0400 8, 31 -- Received: from d01av03.pok.ibm.com (loopback [127.0.0.1]) 8, 31 -- by d01av03.pok.ibm.com (8.14.4/8.13.1/NCO v10.0 AVout) with ESMTP id p53AuLih030356 8, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Jun 2011 07:56:21 -0300 8, 31 -- Received: from d01ml076.pok.ibm.com ([9.63.8.33]) 8, 31 -- by d01av03.pok.ibm.com (8.14.4/8.13.1/NCO v10.0 AVin) with ESMTP id p53AuLxo030346 8, 31 -- for {Microscopy-at-Microscopy.Com} ; Fri, 3 Jun 2011 07:56:21 -0300 8, 31 -- In-Reply-To: {201106031431.p53EVRVO025995-at-ns.microscopy.com} 8, 31 -- References: {201106031431.p53EVRVO025995-at-ns.microscopy.com} 8, 31 -- To: Microscopy-at-Microscopy.Com 8, 31 -- MIME-Version: 1.0 8, 31 -- Subject: Re: [Microscopy] Re: Re: Santovac5 8, 31 -- X-KeepSent: 0CB94DED:776F65C3-852578A4:00502CCD; 8, 31 -- type=4; name=$KeepSent 8, 31 -- X-Mailer: Lotus Notes Release 8.0.2 HF623 January 16, 2009 8, 31 -- Message-ID: {OF0CB94DED.776F65C3-ON852578A4.00502CCD-852578A4.00521350-at-us.ibm.com} 8, 31 -- From: Darrell Miles {milesd-at-us.ibm.com} 8, 31 -- Date: Fri, 3 Jun 2011 10:56:31 -0400 8, 31 -- X-MIMETrack: Serialize by Router on D01ML076/01/M/IBM(Release 8.5.2FP1 ZX852FP1HF6|May 2, 2011) at 8, 31 -- 06/03/2011 10:56:32, 8, 31 -- Serialize complete at 06/03/2011 10:56:32 8, 31 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
Yeah, it's a slippery slope to a sticky mess. Phil
} Geez Phil First it's Santovac, next you'll graduate to Apiezon L, or, } help us, N. Will it stop before the bottom, before Vestopal W or (psst, } really I have some legitimate) Epon 812.... } } I'm gettin' worried phil ;). } } } paul } -- } Paul R. Hazelton, PhD } Gastroenteric Diseases Study Group } University of Manitoba } Department of Medical Microbiology } 511 Basic Medical Sciences Building } 730 William Avenue } Winnipeg, Manitoba, Canada, R3E 3J7 } e-mail: paul_hazelton-at-umanitoba.ca } Phone: 204-789-3313 (w) } 204-489-6924 (h) } Cell: 204-781-6982 } Fax: 204-789-3926
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 3, 29 -- From oshel1pe-at-cmich.edu Fri Jun 3 10:18:34 2011 3, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53FIXua025610 3, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 10:18:34 -0500 3, 29 -- Received: from egatea.central.cmich.local ([141.209.15.74]) 3, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p53FIOPt012038 3, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 11:18:33 -0400 3, 29 -- Received: from cas1.central.cmich.local ([141.209.15.75]) by egatea.central.cmich.local with Microsoft SMTPSVC(6.0.3790.4675); 3, 29 -- Fri, 3 Jun 2011 11:17:44 -0400 3, 29 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 3, 29 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Fri, 3 Jun 3, 29 -- 2011 11:17:44 -0400 3, 29 -- MIME-Version: 1.0 3, 29 -- Message-ID: {a06240801ca0eab88e31b-at-[141.209.160.249]} 3, 29 -- In-Reply-To: {201106031433.p53EXogM030250-at-ns.microscopy.com} 3, 29 -- References: {201106031433.p53EXogM030250-at-ns.microscopy.com} 3, 29 -- Date: Fri, 3 Jun 2011 11:17:42 -0400 3, 29 -- To: {Microscopy-at-microscopy.com} 3, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 3, 29 -- Subject: [Microscopy] Re: Re: Santovac5 3, 29 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 3, 29 -- X-Originating-IP: [141.209.160.249] 3, 29 -- X-OriginalArrivalTime: 03 Jun 2011 15:17:44.0537 (UTC) FILETIME=[636E0C90:01CC2201] 3, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 3, 29 -- X-Spam-Score: -4.20 () [Hold at 6.00] L_EXCH_MF,RDNS_NONE,Bayes(0.0001:-0.5) 3, 29 -- X-CanIt-Geo: ip=141.209.15.74; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 3, 29 -- X-CanItPRO-Stream: default 3, 29 -- X-Canit-Stats-ID: 02EPrixlN - 9b8c4667a99a - 20110603 3, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Several years ago a faculty member got into a panic because he'd read about the supposed suitability of osmium tetroxide as a weapon of terrorism, and wanted to lock up our vast stock of the reagent (10 x 1 mL vials). Fortunately this didn't happen, and we didn't have to pay to have corn oil showers installed throughout the building...
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Unfortunate Book Titles: Handbook for the Limbless Punishment by Robin Banks Motorcycling for Beginners by Geoff Carless Illustrated History of Gymnastics by John Goodbody Care for your Kitten by Anna Mews 1587. A Year of No Importance The English. Are They Human? Dr G.J. Rennier, 1931
==============================Original Headers============================== 7, 19 -- From jehrman-at-mta.ca Fri Jun 3 11:04:56 2011 7, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.30]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53G4uUV010012 7, 19 -- for {Microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 11:04:56 -0500 7, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:54143) 7, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 7, 19 -- (Exim 4.71) 7, 19 -- (envelope-from {jehrman-at-mta.ca} ) 7, 19 -- id 1QSWrn-0001Jr-VB 7, 19 -- for Microscopy-at-microscopy.com; Fri, 03 Jun 2011 13:04:55 -0300 7, 19 -- Message-ID: {4DE90624.9070302-at-mta.ca} 7, 19 -- Date: Fri, 03 Jun 2011 13:04:52 -0300 7, 19 -- From: James Ehrman {jehrman-at-mta.ca} 7, 19 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 7, 19 -- MIME-Version: 1.0 7, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 7, 19 -- Subject: [Microscopy] Re: Santovac5 7, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Randy, We had such a problem on a Krios where film was used, and the Gatan service person was able to clean the oil and other crud off the scintillator by carefully flowing solvent (I think ethanol, but I'm not sure at this point) over it. It was a pretty tricky process, so I would definitely call the appropriate service person rather than trying it yourself. You might be able to do it yourself once you've seen it done. Yours, Bill
X-from: randy-nessler-at-uiowa.edu To: William.F.Tivol-at-aero.org
And what if the temperature that the heat cycle obtains does not cause sublimation of the contaminants?
I've had a couple of off-line replies that are curious about this as well. Thanks, Randy
Of course! That's why there is usually a control to turn off the Peltier when the camera is not in use, or even a heater to outgas it periodically. How much you need to use these controls, though, depends on how clean you vacuum is.
A. John Mardinly, ASU
For those running cooled CCD cameras on your TEM's, do you have a problem with them acting like cold traps, with the surface of the scintillator becoming contaminated?
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53MdjGp016187 for {MicroscopyListserverArchive2-at-microscopy.com} ; Fri, 3 Jun 2011 17:39:45 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p53Mdjec016185; Fri, 3 Jun 2011 17:39:45 -0500
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Libbee
Organization: LBNL
Title-Subject: [Filtered] Clamshell Cu TEM grids
Message: Who sells Cu clamshell TEM grids? Login Host: 198.128.25.247 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Jun 3 17:39:45 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p53Mdjgg016182 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 17:39:45 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 467B23B47D7 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 17:39:45 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id T6Ww5z3cTpFD for {microscopy-at-microscopy.com} ; 7, 25 -- Fri, 3 Jun 2011 17:39:44 -0500 (CDT) 7, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id ADEA73B47CC 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 3 Jun 2011 17:39:44 -0500 (CDT) 7, 25 -- Message-ID: {4DE962B0.3080902-at-microscopy.com} 7, 25 -- Date: Fri, 03 Jun 2011 17:39:44 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: mlibbee-at-gmail.com 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Clamshell Cu TEM grids 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hrd-at-uoregon.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hrd-at-uoregon.edu Name: Hannah Dietterich
Organization: University of Oregon
Title-Subject: [Filtered] Help: Epo-fix epoxy did not set in EPMA mount
Message: Hello,
I have an acrylic mount for the electron microprobe with wells into which I have placed mineral samples. I then filled the wells with epo-fix cool mounting epoxy, but after 2 days of drying at room temperature and some time on a hot plate, the epoxy is still soft at the base of the wells. Fortunately, it appears to be hardened at the top where the samples are mounted. I think it is likely that the epoxy was not mixed well enough, but my samples are not replaceable so I am looking for a way to rescue the mount. Please respond if you have any ideas or suggestions as to how to get the existing epoxy to harden fully, or how to effectively plug the wells to allow the mount to be placed in the microprobe.
Thank you, Hannah Dietterich
-- Hannah R. Dietterich Ph.D. Candidate Department of Geological Sciences University of Oregon
When embedding particulate samples such as cell pellets, nanoparticles, etc. in LR White, I get a crumbling sample. That is the particles are embedded, but sample does not maintain a solid block. I don't have this problem with pieces of solid tissue. Has anyone out there encountered this problem?
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 3, 23 -- From tbargar-at-unmc.edu Mon Jun 6 10:30:56 2011 3, 23 -- Received: from zixvpm01.unmc.edu (mysecuremail.unmc.edu [192.198.54.126] (may be forged)) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p56FUtpO002670 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 10:30:55 -0500 3, 23 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 3, 23 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 7D9FF109045C 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 10:30:55 -0500 (CDT) 3, 23 -- Received: from unmcnotes01.unmc.edu (unknown [10.8.51.5]) 3, 23 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 55B4F1090465 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 10:30:54 -0500 (CDT) 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- MIME-Version: 1.0 3, 23 -- Subject: Problem with LR White 3, 23 -- X-KeepSent: 8933F56B:F6FCC749-862578A7:0054C5F1; 3, 23 -- type=4; name=$KeepSent 3, 23 -- X-Mailer: Lotus Notes Release 8.0.2 August 07, 2008 3, 23 -- Message-ID: {OF8933F56B.F6FCC749-ON862578A7.0054C5F1-862578A7.00553A17-at-unmc.edu} 3, 23 -- From: Tom W Bargar {tbargar-at-unmc.edu} 3, 23 -- Date: Mon, 6 Jun 2011 10:30:54 -0500 3, 23 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 06/06/2011 10:30:54 3, 23 -- AM, 3, 23 -- Serialize complete at 06/06/2011 10:30:54 AM 3, 23 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
One possibility is that the polymerization occurred at different rates in the cells or nanoparticles as compared to free plastic in surrounding areas. This is especially obvious when using a UV polymerized resin like LR Gold but I suspect it happens in all resins even heat-polymerized LR White. Uneven polymerization can result from the UV light or heat not penetrating evenly or differential infiltration of one resin component into the tissue. Another possibility is that the tissue regions shrink during polymerization and pull away from the surrounding plastic. If it was easy, even the heathen molecular geneticists could do the magic; this is why only the best scientists become microscopists.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu] Sent: Monday, June 06, 2011 10:32 AM To: Phillips, Thomas E.
When embedding particulate samples such as cell pellets, nanoparticles, etc. in LR White, I get a crumbling sample. That is the particles are embedded, but sample does not maintain a solid block. I don't have this problem with pieces of solid tissue. Has anyone out there encountered this problem?
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 3, 23 -- From tbargar-at-unmc.edu Mon Jun 6 10:30:56 2011 3, 23 -- Received: from zixvpm01.unmc.edu (mysecuremail.unmc.edu [192.198.54.126] (may be forged)) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p56FUtpO002670 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 10:30:55 -0500 3, 23 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 3, 23 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 7D9FF109045C 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 10:30:55 -0500 (CDT) 3, 23 -- Received: from unmcnotes01.unmc.edu (unknown [10.8.51.5]) 3, 23 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 55B4F1090465 3, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 10:30:54 -0500 (CDT) 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- MIME-Version: 1.0 3, 23 -- Subject: Problem with LR White 3, 23 -- X-KeepSent: 8933F56B:F6FCC749-862578A7:0054C5F1; 3, 23 -- type=4; name=$KeepSent 3, 23 -- X-Mailer: Lotus Notes Release 8.0.2 August 07, 2008 3, 23 -- Message-ID: {OF8933F56B.F6FCC749-ON862578A7.0054C5F1-862578A7.00553A17-at-unmc.edu} 3, 23 -- From: Tom W Bargar {tbargar-at-unmc.edu} 3, 23 -- Date: Mon, 6 Jun 2011 10:30:54 -0500 3, 23 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 06/06/2011 10:30:54 3, 23 -- AM, 3, 23 -- Serialize complete at 06/06/2011 10:30:54 AM 3, 23 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
==============================Original Headers============================== 16, 31 -- From PhillipsT-at-missouri.edu Mon Jun 6 11:39:37 2011 16, 31 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p56Gdb08019867 16, 31 -- for {microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 11:39:37 -0500 16, 31 -- X-IronPort-Anti-Spam-Filtered: true 16, 31 -- X-IronPort-Anti-Spam-Result: Ah4BAM0B7U3Raua1/2dsb2JhbABNBhCXMo5id8BjiHQCgy+CcASGdJkKUw 16, 31 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) 16, 31 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 06 Jun 2011 11:39:37 -0500 16, 31 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by 16, 31 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Mon, 6 Jun 2011 16, 31 -- 11:39:37 -0500 16, 31 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 16, 31 -- To: "tbargar-at-unmc.edu" {tbargar-at-unmc.edu} , 16, 31 -- "microscopy-at-microscopy.com" 16, 31 -- {microscopy-at-microscopy.com} 16, 31 -- Date: Mon, 6 Jun 2011 11:39:36 -0500 16, 31 -- Subject: RE: [Microscopy] Problem with LR White 16, 31 -- Thread-Topic: [Microscopy] Problem with LR White 16, 31 -- Thread-Index: AcwkXuIoOVIF/RX/Qx6KX0SgUByx9AACJDyw 16, 31 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF267BF9D562-at-UM-EMAIL05.um.umsystem.edu} 16, 31 -- References: {201106061532.p56FW1tl003629-at-ns.microscopy.com} 16, 31 -- In-Reply-To: {201106061532.p56FW1tl003629-at-ns.microscopy.com} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- acceptlanguage: en-US 16, 31 -- Content-Type: text/plain; charset="us-ascii" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p56Gdb08019867 ==============================End of - Headers==============================
I am trying to view live cells using a confocal laser scanning microscope and experience immense photobleaching with my cell while trying to do the z-slices. Any idea if there is a certain "antifade" medium I could suspend the live cells with?
==============================Original Headers============================== 1, 21 -- From dmywong-at-ucdavis.edu Mon Jun 6 17:16:07 2011 1, 21 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p56MG6qQ011556 1, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 17:16:06 -0500 1, 21 -- Received: by pzk4 with SMTP id 4so2578738pzk.0 1, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Jun 2011 15:16:05 -0700 (PDT) 1, 21 -- Received: by 10.68.38.163 with SMTP id h3mr2308777pbk.196.1307398565563; 1, 21 -- Mon, 06 Jun 2011 15:16:05 -0700 (PDT) 1, 21 -- Received: from [192.168.1.114] ([128.120.242.213]) 1, 21 -- by mx.google.com with ESMTPS id y2sm3973984pbi.19.2011.06.06.15.16.04 1, 21 -- (version=TLSv1/SSLv3 cipher=OTHER); 1, 21 -- Mon, 06 Jun 2011 15:16:04 -0700 (PDT) 1, 21 -- Message-Id: {BA342407-F2F0-4793-A02F-EFFEDA43BD87-at-ucdavis.edu} 1, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 1, 21 -- To: Microscopy-at-microscopy.com 1, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 1, 21 -- Content-Transfer-Encoding: 7bit 1, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 1, 21 -- Subject: Photobleaching prevention for live cells 1, 21 -- Date: Mon, 6 Jun 2011 15:16:03 -0700 1, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
I am looking for a program that could quantify fluorescence for confocal imaging. Do you know of any? ______________________________ Diana M. Wong Graduate Student
Franz Group University of California, Davis Department of Chemistry One Shields Ave Davis, CA 95616 dmywong-at-ucdavis.edu http://chemgroups.ucdavis.edu/~franz/
==============================Original Headers============================== 6, 21 -- From dmywong-at-ucdavis.edu Mon Jun 6 17:17:49 2011 6, 21 -- Received: from mail-pw0-f41.google.com (mail-pw0-f41.google.com [209.85.160.41]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p56MHml5012446 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 17:17:48 -0500 6, 21 -- Received: by pwi12 with SMTP id 12so2836057pwi.0 6, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Jun 2011 15:17:47 -0700 (PDT) 6, 21 -- Received: by 10.68.32.231 with SMTP id m7mr2122308pbi.200.1307398667654; 6, 21 -- Mon, 06 Jun 2011 15:17:47 -0700 (PDT) 6, 21 -- Received: from [192.168.1.114] ([128.120.242.213]) 6, 21 -- by mx.google.com with ESMTPS id k4sm3971929pbl.43.2011.06.06.15.17.46 6, 21 -- (version=TLSv1/SSLv3 cipher=OTHER); 6, 21 -- Mon, 06 Jun 2011 15:17:46 -0700 (PDT) 6, 21 -- Message-Id: {888E6CFA-5CE4-4277-A843-0B98DB332D45-at-ucdavis.edu} 6, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 6, 21 -- To: Microscopy-at-microscopy.com 6, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 6, 21 -- Content-Transfer-Encoding: 7bit 6, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 6, 21 -- Subject: Fluorescence Quantification 6, 21 -- Date: Mon, 6 Jun 2011 15:17:45 -0700 6, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yun.peng-at-ge.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: yun.peng-at-ge.com Name: Peng, Yun
Organization: GE-GRC
Title-Subject: [Filtered] About cross section study by SEM
Message: I am a relative newcomer to the Microscopy field and need some advice for the sample preparation for cross section study of fiber material. This kind of film material (Polyethylene, polypropylene etc.) has pores structure and orientation. The thickness is around 30-50 microns. It is too tough to break in liquid nitrogen. We had ever tried to cut it in liquid nitrogen by a blade. But the pores structure is some distorted. meanwhile, I found cryostat microtome doesnÂ’t work for our case. Is there any trick for this case? I had ever got some information from this website before. I still have some questions about it.
1. Insert the material in a small diameter tube (thin drinking straws are ideal). Cut the straw down to about 3cms tall. Block one end with wax, modeling clay or similar material. Q&A: Is wax, modeling clay or similar material only used to block end? If it is also filled in the big gap between straw and film materials? 2. Using a syringe force water into the straw and block the end as above. Q&A: Is water filled in the big gap between straw and film? 3. Drop the straw into liquid nitrogen then follow method A part 2 above. Q&A: should we cut the sample by blade or break it by tweezers?
I am looking forward your reply and give me a hand. I appreciate it.
I don't have any comments about the methods you list below, but I would be inclined to try a typical metallurgical cross-section. Mount the sample in a material with similar hardness (epoxy may work fine), and grind and polish to achieve your cross-section. There should be plenty of facilities and people at GE-GRC that are familiar with metallurgical sectioning. Good luck.
Diane ___________________________________________
Diane Ciaburri Materials Analysis General Dynamics 100 Plastics Ave., Rm 2517A Pittsfield MA 01201 phone: (413) 494-3430 email: diane.ciaburri-at-gd-ais.com ___________________________________________
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, June 07, 2011 8:30 AM To: Ciaburri, Diane A.
Hi All,
Later this year I'll be leaving Purdue for a new challenge. The following position will become available:
Director, EM Facility/Project Leader, Structural Virology EM Studies – West Lafayette – Biological Sciences
Develop and maintain the EM Facility as a world-class resource allowing Purdue’s Structure Group to continue to function as a world leader in structural biology. Applicants should apply online at www.purdue.edu/jobs and reference posting number 1100885.
Required: • Master’s degree in Science. • Minimum of five years of experience in conventional transmission electron microscopy. • Superior organizational skills. • Ability to manage a large, multi-user research laboratory with no immediate supervision. • Ability to solicit and evaluate advice from and interact with appropriate professionals to maintain the equipment in optimal condition. • Capable of using independent judgment for purchases. • Ability to quantitatively analyze scientific results. • Above-average attention to detail and be able to communicate effectively with faculty, staff, and students. • Ability to research or develop new technologies and be capable of evaluation and recommending new instrumentation. • Willing to learn/travel and keep abreast of new technology. • Ability to use and teach others to use all the equipment related to the EM facility. • Ability to use and teach others in the techniques of 3D image reconstructions. • Ability to manage the managerial aspects of this position with the research and supervisory component. Preferred: • PhD in Science. • Background in life sciences. • Formal training in transmission electron microscopy. • Experience in cryo-electron microscopy and/or cryo-electron tomography. • Experience in managing the operations of a research facility, troubleshooting with high precision instruments, and teaching students and postdoctoral researchers and staff how to use electron microscopes. • Experience in managing service contracts and interacting with service engineers. • Experience with three-dimensional image analysis and publication in peer-reviewed journals. Additional Information: • A background check will be required for employment in this position. • FLSA: Exempt (Not Eligible For Overtime) • Retirement Eligibility: Fidelity Contributions Immediately • Purdue University is an equal opportunity, equal access, affirmative action employer.
Cheers, Paul
-- Paul Chipman Director, Biological Electron Microscopy Facility Purdue University 765-494-1487
==============================Original Headers============================== 10, 20 -- From paulrc-at-bilbo.bio.purdue.edu Tue Jun 7 10:24:43 2011 10, 20 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 10, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p57FOgDd030844 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 10:24:43 -0500 10, 20 -- Received: from dhcp44-100-fixed.bio.purdue.edu (dhcp44-100-fixed.bio.purdue.edu [128.210.44.100]) 10, 20 -- (authenticated bits=0) 10, 20 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-auth.smtp.purdue.edu) with ESMTP id p57FOgQn029587 10, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 10, 20 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 11:24:42 -0400 10, 20 -- Message-ID: {4DEE42BA.50701-at-bilbo.bio.purdue.edu} 10, 20 -- Date: Tue, 07 Jun 2011 11:24:42 -0400 10, 20 -- From: "Chipman, Paul R" {paulrc-at-bilbo.bio.purdue.edu} 10, 20 -- User-Agent: Thunderbird 2.0.0.14 (Macintosh/20080421) 10, 20 -- MIME-Version: 1.0 10, 20 -- To: microscopy-at-microscopy.com 10, 20 -- Subject: Open Position at Purdue Univ. 10, 20 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 20 -- Content-Transfer-Encoding: 8bit 10, 20 -- X-PMX-Version: 5.5.9.388399 10, 20 -- X-PerlMx-Virus-Scanned: Yes ==============================End of - Headers==============================
I wrote the paper that you discuss so let me try to help you? The basic idea is a media stiffens the fibre and forces it to fracture. Firstly to answer your questions.
1. the wax is simply to block one end
2. water may be used to provide a solid interface that will fracture cleanly
3. use strong tweezers or grippers (in the UK we would say pliers) to bend the straw unit until fracture occurs.
OR
A infiltrate the fibres within the drinking with straw a water soluble carbon solution. B Once fully infiltrated block one end with wax as above; allow to dry fully (hours). C Place in liquid nitrogen until it stops bubbling. D Remove and with two strong grippers (UK we would say pliers) bend the straw unit to fracture.
We have many times conducted investigation of fibre internal structure by using this method.
Come back to me if you need more?
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Email: yun.peng-at-ge.com Name: Peng, Yun
Organization: GE-GRC
Title-Subject: [Filtered] About cross section study by SEM
Message: I am a relative newcomer to the Microscopy field and need some advice for the sample preparation for cross section study of fiber material. This kind of film material (Polyethylene, polypropylene etc.) has pores structure and orientation. The thickness is around 30-50 microns. It is too tough to break in liquid nitrogen. We had ever tried to cut it in liquid nitrogen by a blade. But the pores structure is some distorted. meanwhile, I found cryostat microtome doesnÂ’t work for our case. Is there any trick for this case? I had ever got some information from this website before. I still have some questions about it.
1. Insert the material in a small diameter tube (thin drinking straws are ideal). Cut the straw down to about 3cms tall. Block one end with wax, modeling clay or similar material. Q&A: Is wax, modeling clay or similar material only used to block end? If it is also filled in the big gap between straw and film materials? 2. Using a syringe force water into the straw and block the end as above. Q&A: Is water filled in the big gap between straw and film? 3. Drop the straw into liquid nitrogen then follow method A part 2 above. Q&A: should we cut the sample by blade or break it by tweezers?
I am looking forward your reply and give me a hand. I appreciate it.
I am not sure if someone responded to you as confocal list might be a better place to post these questions. Nevertheless, here are few things you might consider.
1) First, make sure your system is well aligned and in the peak performance. Check pinhole alignment, make sure lens is clean, etc.
2) Make sure your system is at most sensitive settings. For live cell imaging, it is always a compromise of many factors. Here are few things to look into: a) check the PMTs setting should be at the most sensitive level with acceptable noise level. b) set pinhole to somewhat larger. Depending on the lens you use, this could be as large as 2-3 airy disk size. c) reduce resolution by dropping zoom value. Your photobleach/toxicity level increases by power of 2 of the zoom value. Pixel value should be match or a bit below the Nyquist value because with live cell imaging (unless you use a well corrected water immersion lens), aberration will cause resolution drop. d) check your average value and scanning speed. Remember, slower the scan, more average, more dosage of light will be put onto your sample. Therefore, reduce those parameters if you can afford it (depending on your signal-noise level requirement).
e) Use the least amount of excitation light (laser setting) as you could afford. f) Use a more photo-stable dye.
3) If all above failed, you could try some chemicals to reduce photobleaching rate. We tested a whole bunch of them. One seems working the best is using Trolox at ~300uM either overnight or 2 hrs before imaging (added to the media). But remember this might interfere with the biological process you are investigating. Therefore, please do proper control first.
As to fluorescence quantification, I do not know what you want to do. ImageJ comes free (or you can search Fuji which is imageJ with some plugin prepackaged in). There are many plugins for various purposes. So you need to do some home work to find the right plugin for your quantification.
Best wishes,
xuejun
Xuejun SUN, Ph.D. Dept. Exp. Oncology Cross Cancer Institute 11560 University Ave. Edmonton Alberta, T6G1Z2
-----Original Message----- X-from: dmywong-at-ucdavis.edu [mailto:dmywong-at-ucdavis.edu] Sent: Monday, June 06, 2011 4:25 PM To: xjsun-at-ualberta.ca
I am trying to view live cells using a confocal laser scanning microscope and experience immense photobleaching with my cell while trying to do the z-slices. Any idea if there is a certain "antifade" medium I could suspend the live cells with?
==============================Original Headers============================== 1, 21 -- From dmywong-at-ucdavis.edu Mon Jun 6 17:16:07 2011 1, 21 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p56MG6qQ011556 1, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 6 Jun 2011 17:16:06 -0500 1, 21 -- Received: by pzk4 with SMTP id 4so2578738pzk.0 1, 21 -- for {Microscopy-at-microscopy.com} ; Mon, 06 Jun 2011 15:16:05 -0700 (PDT) 1, 21 -- Received: by 10.68.38.163 with SMTP id h3mr2308777pbk.196.1307398565563; 1, 21 -- Mon, 06 Jun 2011 15:16:05 -0700 (PDT) 1, 21 -- Received: from [192.168.1.114] ([128.120.242.213]) 1, 21 -- by mx.google.com with ESMTPS id y2sm3973984pbi.19.2011.06.06.15.16.04 1, 21 -- (version=TLSv1/SSLv3 cipher=OTHER); 1, 21 -- Mon, 06 Jun 2011 15:16:04 -0700 (PDT) 1, 21 -- Message-Id: {BA342407-F2F0-4793-A02F-EFFEDA43BD87-at-ucdavis.edu} 1, 21 -- From: "Diana M. Wong" {dmywong-at-ucdavis.edu} 1, 21 -- To: Microscopy-at-microscopy.com 1, 21 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 1, 21 -- Content-Transfer-Encoding: 7bit 1, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 1, 21 -- Subject: Photobleaching prevention for live cells 1, 21 -- Date: Mon, 6 Jun 2011 15:16:03 -0700 1, 21 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you.
==============================Original Headers============================== 22, 28 -- From xjsun-at-ualberta.ca Tue Jun 7 12:02:10 2011 22, 28 -- Received: from scutum.calgaryhealthregion.ca (scutum.calgaryhealthregion.ca [198.161.2.98]) 22, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p57H2APj032057 22, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 12:02:10 -0500 22, 28 -- Received: from scutum.calgaryhealthregion.ca (unknown [127.0.0.1]) 22, 28 -- by IMSA (Postfix) with ESMTP id 2E55820F0F8; 22, 28 -- Tue, 7 Jun 2011 11:02:04 -0600 (MDT) 22, 28 -- Received: from EXHUB1B.crha.bewell.ca (unknown [139.48.198.163]) 22, 28 -- by scutum.calgaryhealthregion.ca (Postfix) with ESMTP id 136A220F0EF; 22, 28 -- Tue, 7 Jun 2011 11:02:04 -0600 (MDT) 22, 28 -- Received: from ccexo129 (139.48.198.12) by exhub1b.crha.bewell.ca 22, 28 -- (139.48.198.163) with Microsoft SMTP Server id 8.2.254.0; Tue, 7 Jun 2011 22, 28 -- 11:02:03 -0600 22, 28 -- From: Xuejun SUN {xjsun-at-ualberta.ca} 22, 28 -- To: {Microscopy-at-microscopy.com} 22, 28 -- CC: {dmywong-at-ucdavis.edu} 22, 28 -- References: {201106062225.p56MP2Gf023413-at-ns.microscopy.com} 22, 28 -- Subject: RE: [Microscopy] Photobleaching prevention for live cells 22, 28 -- Date: Tue, 7 Jun 2011 11:02:02 -0600 22, 28 -- Message-ID: {8AC7DCD5B30F4318A33DFB2D1049DCBC-at-ad.cancerboard.ab.ca} 22, 28 -- MIME-Version: 1.0 22, 28 -- Content-Type: text/plain; charset="us-ascii" 22, 28 -- X-Mailer: Microsoft Office Outlook 11 22, 28 -- Thread-Index: AcwkmJRi9pOovocETBmpuxXnmOnaOwAmSjfA 22, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 22, 28 -- In-Reply-To: {201106062225.p56MP2Gf023413-at-ns.microscopy.com} 22, 28 -- Content-Transfer-Encoding: 8bit 22, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p57H2APj032057 ==============================End of - Headers==============================
I know this issue has surfaced several times, but I am gathering information about researcher's experiences with ultramicrotomes.
Reply to as many questions as you feel comfortable answering.
More specifically:
1. How long have you owned your ultramicrotome? 2. How often it is used? 3. How many different users? 4. How versatile has it been (i.e., suitable to cut different specimen types)? 5. How reliable has it been? 6. Regarding repairs: how was the cost and was it properly repaired? 7. What is the brand name? 8. Would you purchase another one?
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 18 -- From bozzola-at-siu.edu Tue Jun 7 16:09:00 2011 6, 18 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p57L8xvc020406 6, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 16:09:00 -0500 6, 18 -- Received: by iyl8 with SMTP id 8so5462963iyl.0 6, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 07 Jun 2011 14:08:59 -0700 (PDT) 6, 18 -- MIME-Version: 1.0 6, 18 -- Received: by 10.42.147.69 with SMTP id m5mr11127196icv.12.1307480937909; Tue, 6, 18 -- 07 Jun 2011 14:08:57 -0700 (PDT) 6, 18 -- Received: by 10.42.167.193 with HTTP; Tue, 7 Jun 2011 14:08:57 -0700 (PDT) 6, 18 -- Date: Tue, 7 Jun 2011 16:08:57 -0500 6, 18 -- Message-ID: {BANLkTikTAtm3gRJTLJMb=vecEM3RR8KRqQ-at-mail.gmail.com} 6, 18 -- Subject: ultramicrotome questions 6, 18 -- From: John Bozzola {bozzola-at-siu.edu} 6, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 18 -- Content-Type: text/plain; charset=UTF-8 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p57L8xvc020406 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jgould-at-nanotem.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jgould-at-nanotem.com Name: Janyce Gould
Organization: NanoTEM
Title-Subject: [Filtered] Post-doc position opening
Message: Industrial post-doc position in advanced TEM/AEM of semiconductors*
*US Citizenship required Scottsdale, Arizona 1-year contract, extendable to 2nd year
Position entails involvement in multiple government contracts and commercial projects. Work schedule requires total flexibility. Publishing opportunities are restricted but may be available in limited cases.
If interested, please send a letter of application, resume, and a list of three references via email. No phone calls will be accepted or returned.
I’m a graduate student at the University of South Carolina and I’m looking for a lab with a epi-microspectrophotometer, somewhere in the range of ~ 200 miles or so of Columbia, South Carolina, that would be kind and willing enough to discuss the possibility of using it. Or any information on somebody I might contact would also be greatly appreciated.
I’m in the initial stages of considering the possibility of measuring the absorbance spectra of the Daphnia compound eye. I’m interested in knowing the range of spectral sensitivity in the compound eye across multiple species. To the best of my knowledge, most of the literature exploring the spectral sensitivity of compound eyes has employed this instrument.
Thanks Much,
Chris brandonc-at-email.sc.edu
==============================Original Headers============================== 10, 33 -- From rintugr-at-gmail.com Wed Jun 8 12:43:06 2011 10, 33 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p58Hh52p008071 10, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jun 2011 12:43:06 -0500 10, 33 -- Received: by pzk4 with SMTP id 4so433760pzk.0 10, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 08 Jun 2011 10:43:05 -0700 (PDT) 10, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 33 -- d=gmail.com; s=gamma; 10, 33 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 10, 33 -- :content-type:content-transfer-encoding; 10, 33 -- bh=ULYBj2TG6dJfxq3CmmJ+BO/AwqsBFsBUwp+ndFNh9SY=; 10, 33 -- b=KQ0LKSLTblSMCeO2mhOzeiRQFdJEFC7iz+9IZ02HydAm0ox/VV/n4pBiV5oEbL4lzY 10, 33 -- KsfAUN+aAdBvJeMC9YvchC+N6j6dCoM86dZsSZbluiWTn2Y14qdWXM6pDN4E/uqDiLbQ 10, 33 -- 15nRD2SXl0m24yRBm6Mw3vVpqgCY0pHFfXM7Q= 10, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 10, 33 -- d=gmail.com; s=gamma; 10, 33 -- h=mime-version:date:message-id:subject:from:to:content-type 10, 33 -- :content-transfer-encoding; 10, 33 -- b=HdABBjfeTQRZiH/ZesnirOAgk0RhlMkhImq/DOVJMIaRVi6wCbko9pJ8ocLJYHV+UM 10, 33 -- zSB2/rCBu02FcQhidRirZaf5B+pl/PEpwiaNY8I2yAFWTSxOvybM7ZCswhIHYd1mg9Qq 10, 33 -- MiFSBIBPsaRJlZCaePTXlhXs87LV6E4e1Ei9g= 10, 33 -- MIME-Version: 1.0 10, 33 -- Received: by 10.142.147.10 with SMTP id u10mr423168wfd.112.1307554985193; Wed, 10, 33 -- 08 Jun 2011 10:43:05 -0700 (PDT) 10, 33 -- Received: by 10.142.143.18 with HTTP; Wed, 8 Jun 2011 10:43:05 -0700 (PDT) 10, 33 -- Date: Wed, 8 Jun 2011 13:43:05 -0400 10, 33 -- Message-ID: {BANLkTi=3i7w-MXNF2A2kprfEbZpaNUiGAQ-at-mail.gmail.com} 10, 33 -- Subject: help needed epi-microspectrophotometer 10, 33 -- From: Soumitra Ghoshroy {rintugr-at-gmail.com} 10, 33 -- To: Microscopy-at-microscopy.com 10, 33 -- Content-Type: text/plain; charset=UTF-8 10, 33 -- Content-Transfer-Encoding: 8bit 10, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p58Hh52p008071 ==============================End of - Headers==============================
We are in the process of evaluating WDS systems for our SEM-FEG (JEOL 7600F). Does anyone have data comparing the performance of the WDS system from Noran and Oxford? Any comments (positive or negative) are welcomed either on this list or privately off list if you prefer.
We're considering purchasing a new desktop SEM. All of our imaging is done below 15,000x, and all of it is of coated specimens. We have access to a research grade SEM in our building and a field-emission SEM on campus. We want the convenience of our own instrument. How rugged are the desktops, and how dependable are they? We will not be getting a service contract. We would be getting a 15kV system that magnifies to 30,000x with a backscattered detector. We don't have the space for a full sized SEM, nor the budget.
Ed Haller
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119
==============================Original Headers============================== 3, 37 -- From ehaller-at-health.usf.edu Wed Jun 8 16:19:22 2011 3, 37 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p58LJMxD007479 3, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jun 2011 16:19:22 -0500 3, 37 -- X-ASG-Debug-ID: 1307567961-1e0801d10000-1DjkGe 3, 37 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-bin/mark.cgi 3, 37 -- Received: from mailhub1.hscnet.hsc.usf.edu (localhost [127.0.0.1]) 3, 37 -- by hscantispam.health.usf.edu (Spam & Virus Firewall) with ESMTP id F24558B4B776 3, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jun 2011 17:19:21 -0400 (EDT) 3, 37 -- Received: from mailhub1.hscnet.hsc.usf.edu ([10.119.4.12]) by hscantispam.health.usf.edu with ESMTP id ad9UKiIrj1AUVE53 for {Microscopy-at-microscopy.com} ; Wed, 08 Jun 2011 17:19:21 -0400 (EDT) 3, 37 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 3, 37 -- Received: from EXCH05.hscnet.hsc.usf.edu (10.119.4.45) by 3, 37 -- mailhub1.hscnet.hsc.usf.edu (10.119.4.12) with Microsoft SMTP Server (TLS) id 3, 37 -- 8.2.213.0; Wed, 8 Jun 2011 17:19:22 -0400 3, 37 -- Received: from EXCH03.hscnet.hsc.usf.edu ([fe80::94db:1b22:fb18:d0b4]) by 3, 37 -- EXCH05.hscnet.hsc.usf.edu ([fe80::7974:53df:c461:2a13%14]) with mapi id 3, 37 -- 14.01.0270.001; Wed, 8 Jun 2011 17:19:21 -0400 3, 37 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 3, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 37 -- X-ASG-Orig-Subj: Asking for experiences from owners of Desktop SEMs 3, 37 -- Subject: Asking for experiences from owners of Desktop SEMs 3, 37 -- Thread-Topic: Asking for experiences from owners of Desktop SEMs 3, 37 -- Thread-Index: AcwmHf67bWOGnVEJQQKS3ZinXRtZtw== 3, 37 -- Date: Wed, 8 Jun 2011 21:19:20 +0000 3, 37 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C814EFC55F-at-EXCH03.hscnet.hsc.usf.edu} 3, 37 -- Accept-Language: en-US 3, 37 -- Content-Language: en-US 3, 37 -- X-MS-Has-Attach: 3, 37 -- X-MS-TNEF-Correlator: 3, 37 -- x-originating-ip: [10.119.4.53] 3, 37 -- Content-Type: text/plain; charset="us-ascii" 3, 37 -- MIME-Version: 1.0 3, 37 -- X-Barracuda-Connect: UNKNOWN[10.119.4.12] 3, 37 -- X-Barracuda-Start-Time: 1307567961 3, 37 -- X-Barracuda-Virus-Scanned: by USF Health AntiSpam System at health.usf.edu 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p58LJMxD007479 ==============================End of - Headers==============================
We have also had success with fracturing hair specimens using the technique that Steve described, using the mountant for cryostat microtomy (when carbon wasn't available). The specimen was then rinsed in water then alcohol to dry. And if in a hurry, wrapping in aluminium foil (or some small zip-lock bags, some will completely shatter) has also achieved success.
Steve's reference to placing in liquid nitrogen until it stops bubbling was the critical step. We found that it is very important to have the specimen as cold as possible to get it to snap rather than just keep bending.
I can confirm that the 'cracked' transverse section is far superior to any we achieved previously by cutting.
Kind regards Naomi
} } } {protrain-at-emcourses.com} 6/8/2011 1:43 am } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi
I wrote the paper that you discuss so let me try to help you? The basic idea is a media stiffens the fibre and forces it to fracture. Firstly to answer your questions.
1. the wax is simply to block one end
2. water may be used to provide a solid interface that will fracture cleanly
3. use strong tweezers or grippers (in the UK we would say pliers) to bend the straw unit until fracture occurs.
OR
Ainfiltrate the fibres within the drinking with straw a water soluble carbon solution. BOnce fully infiltrated block one end with wax as above; allow to dry fully (hours). CPlace in liquid nitrogen until it stops bubbling. DRemove and with two strong grippers (UK we would say pliers) bend the straw unit to fracture.
We have many times conducted investigation of fibre internal structure by using this method.
Come back to me if you need more?
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967
www.emcourses.com
Email: yun.peng-at-ge.com Name: Peng, Yun
Organization: GE-GRC
Title-Subject: [Filtered] About cross section study by SEM
Message: I am a relative newcomer to the Microscopy field and need some
advice for the sample preparation for cross section study of fiber material. This kind of film material (Polyethylene, polypropylene etc.)
has pores structure and orientation. The thickness is around 30-50 microns. It is too tough to break in liquid nitrogen. We had ever tried
to cut it in liquid nitrogen by a blade. But the pores structure is some distorted. meanwhile, I found cryostat microtome doesn’t work for our
case. Is there any trick for this case? I had ever got some information from this website before. I still have
some questions about it.
1. Insert the material in a small diameter tube (thin drinking straws are ideal). Cut the straw down to about 3cms tall. Block one end with
wax, modeling clay or similar material. Q&A: Is wax, modeling clay or similar material only used to block end?
If it is also filled in the big gap between straw and film materials? 2. Using a syringe force water into the straw and block the end as above. Q&A: Is water filled in the big gap between straw and film? 3. Drop the straw into liquid nitrogen then follow method A part 2 above. Q&A: should we cut the sample by blade or break it by tweezers?
I am looking forward your reply and give me a hand. I appreciate it.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jun 7 07:30:21 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p57CULZv025806 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 07:30:21 -0500 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id 849263B76C0 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 07:30:21 -0500 (CDT) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id 7ruddfpo9ZLg for {microscopy-at-microscopy.com} ; 12, 25 -- Tue, 7 Jun 2011 07:30:18 -0500 (CDT) 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 25 -- by znl.com (Postfix) with ESMTPA id A24363B76B0 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 07:30:18 -0500 (CDT) 12, 25 -- Message-ID: {4DEE19DA.9010706-at-microscopy.com} 12, 25 -- Date: Tue, 07 Jun 2011 07:30:18 -0500 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: yun.peng-at-ge.com 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 -- Subject: viaWWW:About cross section study by SEM 12, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed 12, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 27, 28 -- From protrain-at-emcourses.com Tue Jun 7 10:29:38 2011 27, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 27, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p57FTbVO001546 27, 28 -- for {microscopy-at-microscopy.com} ; Tue, 7 Jun 2011 10:29:38 -0500 27, 28 -- Received: from [71.46.49.251] (port=8222 helo=ProtrainPC) 27, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 27, 28 -- (envelope-from {protrain-at-emcourses.com} ) 27, 28 -- id 1QTyDm-000HBH-1N by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Tue, 07 Jun 2011 17:29:35 +0200 27, 28 -- Reply-To: {protrain-at-emcourses.com} 27, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 27, 28 -- To: {yun.peng-at-ge.com} 27, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 27, 28 -- References: {201106071232.p57CWeuP027040-at-ns.microscopy.com} 27, 28 -- In-Reply-To: {201106071232.p57CWeuP027040-at-ns.microscopy.com} 27, 28 -- Subject: RE: [Microscopy] viaWWW:About cross section study by SEM 27, 28 -- Date: Tue, 7 Jun 2011 16:29:17 +0100 27, 28 -- Organization: Protrain 27, 28 -- Message-ID: {012601cc2527$b2b80b60$18282220$-at-com} 27, 28 -- MIME-Version: 1.0 27, 28 -- Content-Type: text/plain; 27, 28 -- charset="iso-8859-1" 27, 28 -- X-Mailer: Microsoft Office Outlook 12.0 27, 28 -- Thread-Index: AcwlDw7NDW8k7n0jQ1+CEvhj+GZQ4AAFr5+w 27, 28 -- Content-Language: en-gb 27, 28 -- X-Scan-Signature: 242bce4a7e4d7be507981b7a34ac953c{132}} 27, 28 -- X-Trace: smtp01.dial-up.net 1QTyDm-000HBH-1N 45a690e612415b1c6848d6073e16d4b0 27, 28 -- Content-Transfer-Encoding: 8bit 27, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p57FTbVO001546 ==============================End of - Headers==============================
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 44, 24 -- From prvs=11411b3a29=naomi_mccallum-at-health.qld.gov.au Wed Jun 8 22:47:18 2011 44, 24 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 44, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p593lH6x032283 44, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 8 Jun 2011 22:47:18 -0500 44, 24 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 44, 24 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id p593kfuW025034 44, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 13:46:41 +1000 44, 24 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 44, 24 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 44, 24 -- with ESMTP id 44, 24 -- {T9d81106718c0a803351e40-at-gwd-mail06.remote.health.qld.gov.au} for 44, 24 -- {Microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 13:46:41 +1000 44, 24 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 44, 24 -- Novell_GroupWise; Thu, 09 Jun 2011 13:46:41 +1000 44, 24 -- Message-Id: {4DF0CEB7.88BE.00AA.1-at-health.qld.gov.au} 44, 24 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 44, 24 -- Date: Thu, 09 Jun 2011 13:46:35 +1000 44, 24 -- From: "Naomi McCallum" {naomi_mccallum-at-health.qld.gov.au} 44, 24 -- To: {Microscopy-at-microscopy.com} 44, 24 -- Subject: Fwd: [Microscopy] RE: viaWWW:About cross section study by SEM 44, 24 -- Mime-Version: 1.0 44, 24 -- Content-Type: text/plain; charset="UTF-8" 44, 24 -- Content-Transfer-Encoding: 8bit 44, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
When trying to get images from our BSD,I can get an image in the "high" range (but with faint white lines extending across the screen that rotate off of horizontal at lower scan speeds), but it does not get bright get enough contrast. When it is turned to "very high" range, there are just a series of black and white horizontal lines that zip across the screen.
Any ideas of what to do?
Thanks so much,
Mark
T. Markham Puckett, Ph.D. Associate Professor of Geology Department of Physics and Earth Science P.O. Box 5130 University of North Alabama Florence, Alabama 35632-0001 “Why, sometimes I’ve believed as many as six impossible things before breakfast.” The White Queen to Alice, Through the Looking-Glass
==============================Original Headers============================== 5, 18 -- From piper1960-at-hotmail.com Thu Jun 9 08:44:20 2011 5, 18 -- Received: from col0-omc3-s12.col0.hotmail.com (col0-omc3-s12.col0.hotmail.com [65.55.34.150]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59DiKoE025408 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 08:44:20 -0500 5, 18 -- Received: from COL117-W2 ([65.55.34.137]) by col0-omc3-s12.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 5, 18 -- Thu, 9 Jun 2011 06:42:04 -0700 5, 18 -- Message-ID: {COL117-W29FF2B7898DD4FF598C68BF650-at-phx.gbl} 5, 18 -- X-Originating-IP: [204.29.102.55] 5, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} 5, 18 -- To: {microscopy-at-microscopy.com} 5, 18 -- Subject: BSD problem 5, 18 -- Date: Thu, 9 Jun 2011 08:42:04 -0500 5, 18 -- Importance: Normal 5, 18 -- Content-Type: text/plain; charset="Windows-1252" 5, 18 -- MIME-Version: 1.0 5, 18 -- X-OriginalArrivalTime: 09 Jun 2011 13:42:04.0848 (UTC) FILETIME=[04C98300:01CC26AB] 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p59DiKoE025408 ==============================End of - Headers==============================
When trying to get images from our BSD,I can get an image in the "high" range (but with faint white lines extending across the screen that rotate off of horizontal at lower scan speeds), but it does not get bright get enough contrast. When it is turned to "very high" range, there are just a series of black and white horizontal lines that zip across the screen.
Any ideas of what to do?
Thanks so much,
Mark
T. Markham Puckett, Ph.D. Associate Professor of Geology Department of Physics and Earth Science P.O. Box 5130 University of North Alabama Florence, Alabama 35632-0001 “Why, sometimes I’ve believed as many as six impossible things before breakfast.” The White Queen to Alice, Through the Looking-Glass
==============================Original Headers============================== 5, 18 -- From piper1960-at-hotmail.com Thu Jun 9 08:54:46 2011 5, 18 -- Received: from col0-omc3-s12.col0.hotmail.com (col0-omc3-s12.col0.hotmail.com [65.55.34.150]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59DiKoE025408 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 08:44:20 -0500 5, 18 -- Received: from COL117-W2 ([65.55.34.137]) by col0-omc3-s12.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 5, 18 -- Thu, 9 Jun 2011 06:42:04 -0700 5, 18 -- Message-ID: {COL117-W29FF2B7898DD4FF598C68BF650-at-phx.gbl} 5, 18 -- X-Originating-IP: [204.29.102.55] 5, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} 5, 18 -- To: {microscopy-at-microscopy.com} 5, 18 -- Subject: BSD problem 5, 18 -- Date: Thu, 9 Jun 2011 08:42:04 -0500 5, 18 -- Importance: Normal 5, 18 -- Content-Type: text/plain; charset="Windows-1252" 5, 18 -- MIME-Version: 1.0 5, 18 -- X-OriginalArrivalTime: 09 Jun 2011 13:42:04.0848 (UTC) FILETIME=[04C98300:01CC26AB] 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p59DiKoE025408 ==============================End of - Headers==============================
Sometimes these types of problems with the BSD can be corrected by doing a 'Black Level Calibration', which, on some microscopes, can be found in the 'CZ Detector Calibration' tab. Sometimes they require this to be done rather frequently to operate optimally. This happens more often with my STEM detector, which is a similar solid state detector.
Bruce Thomson Microscopist, Imaging bthomson-at-21cm.com
21st Century Medicine 14960 Hilton Drive Fontana, CA. 92336 ph. (909) 466-8633 fax (909) 466-8618 www.21cm.com
This message has been scanned for any potential malware.
==============================Original Headers============================== 12, 34 -- From bthomson-at-21cm.com Thu Jun 9 11:39:36 2011 12, 34 -- Received: from p02c12o141.mxlogic.net (p02c12o141.mxlogic.net [208.65.145.74]) 12, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59GdZeU020048 12, 34 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 11:39:36 -0500 12, 34 -- Received: from unknown [74.212.219.34] (EHLO 21cm.com) 12, 34 -- by p02c12o141.mxlogic.net(mxl_mta-6.10.0-2) 12, 34 -- with ESMTP id 747f0fd4.0.1336.00-374.3430.p02c12o141.mxlogic.net (envelope-from {bthomson-at-21cm.com} ); 12, 34 -- Thu, 09 Jun 2011 10:39:36 -0600 (MDT) 12, 34 -- X-MXL-Hash: 4df0f7483a7dc168-22a0e867da0a11cf6b304f1c2e1635937d59d75f 12, 34 -- Content-class: urn:content-classes:message 12, 34 -- MIME-Version: 1.0 12, 34 -- Content-Type: text/plain; 12, 34 -- charset="us-ascii" 12, 34 -- Subject: Re: BSD problem 12, 34 -- Date: Thu, 9 Jun 2011 09:39:11 -0700 12, 34 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 34 -- Message-ID: {824E5F5FF9A5E74480853FD0B40C4BBC244655-at-Server1.21CM.local} 12, 34 -- X-MS-Has-Attach: 12, 34 -- X-MS-TNEF-Correlator: 12, 34 -- Thread-Topic: Re: BSD problem 12, 34 -- Thread-Index: Acwmw8LMCKQCgMz6SD6ZMpB8DpFnfg== 12, 34 -- From: "Bruce Thomson" {bthomson-at-21cm.com} 12, 34 -- To: {microscopy-at-microscopy.com} 12, 34 -- X-Processed-By: Rebuild v2.0-0 12, 34 -- X-Spam: [F=0.2000000000; CM=0.500; S=0.200(2010122901)] 12, 34 -- X-MAIL-FROM: {bthomson-at-21cm.com} 12, 34 -- X-SOURCE-IP: [74.212.219.34] 12, 34 -- X-AnalysisOut: [v=1.0 c=1 a=TKFq8H8yXD8A:10 a=BLceEmwcHowA:10 a=kj9zAlcOel] 12, 34 -- X-AnalysisOut: [0A:10 a=rpGmfqynNp5hD1BdSTXp5A==:17 a=c38n3t_TAAAA:8 a=KcL] 12, 34 -- X-AnalysisOut: [F8TGpaGE7_tRLGksA:9 a=CjuIK1q_8ugA:10 a=WJ_dtGxla2IA:10 a=] 12, 34 -- X-AnalysisOut: [M3OdVr6-8VEA:10 a=QEXaimFis7IA:10 a=i_Q9QRP--P4A:10 a=fD4z] 12, 34 -- X-AnalysisOut: [NOj7GI4A:10 a=IXW8o0AqNScA:10] 12, 34 -- Content-Transfer-Encoding: 8bit 12, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p59GdZeU020048 ==============================End of - Headers==============================
Am working with an engineer on a proposal that involves predictive modeling: processes and procedures for silicone wafer etching and design. I have been included because they wish to monitor etchings via some kind of imager. Although this is outside of my level of expertise, the intrigue has brought me into the project. Now I need to discover our options and hope turn-key systems, at affordable prices, are available. There are a number of parameters and restrictions and are listed here.
-object is a 6" silicone wafer; submerged in/out of 5% HF acid with the imaging taking place remote to the bath. Robot would likely pull out the wafer and position on an imager stage (or the equivalent) then return to bath, etc. -most of the 6" disc will need to be imaged; we realize that fields will be tiled together -resolution: ~10um. -capture rate is a few images each hour; maybe more but no video rate. -wish to be able to display image sets in 3-D (much like SEMs) -capital limit at ~$15K
Thanks so much for suggestions.
Steve
-- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
==============================Original Headers============================== 7, 18 -- From steven.samuelsson-at-sri.com Thu Jun 9 12:11:11 2011 7, 18 -- Received: from mail1.sri.com (newmail.SRI.COM [128.18.30.17]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59HBB1d003903 7, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 12:11:11 -0500 7, 18 -- MIME-version: 1.0 7, 18 -- Content-transfer-encoding: 7BIT 7, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 7, 18 -- Received: from Steves-iMac.local ([unknown] [128.18.51.104]) 7, 18 -- by mail.sri.com (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built 7, 18 -- Jul 30 2009)) with ESMTP id {0LMJ00LFC92NXL40-at-mail.sri.com} for 7, 18 -- Microscopy-at-microscopy.com; Thu, 09 Jun 2011 10:11:11 -0700 (PDT) 7, 18 -- Message-id: {4DF0FEAF.7080301-at-sri.com} 7, 18 -- Date: Thu, 09 Jun 2011 10:11:11 -0700 7, 18 -- From: Steven Samuelsson {steven.samuelsson-at-sri.com} 7, 18 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.2.17) 7, 18 -- Gecko/20110414 Thunderbird/3.1.10 7, 18 -- To: Microscopy-at-microscopy.com 7, 18 -- Subject: Suggestions for a 3-D Imager ==============================End of - Headers==============================
Could it be that the particle/cell loading of the resin is too high? Or that the dispersion of the particles in the resin is not uniform? If particles touch each other, or nearly touch, a composite resin/particle material is often very brittle, and if the interfacial bond with the particles is not inherently strong, the material can be dreadfully fragile. (This comment is not based on LR White resins specifically.) -------Roy Arrowood
==============================Original Headers============================== 3, 24 -- From arrowood-at-utep.edu Thu Jun 9 12:54:32 2011 3, 24 -- Received: from itdseciron01.utep.edu (itdseciron01.utep.edu [129.108.0.53]) 3, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59HsWGa020432 3, 24 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 12:54:32 -0500 3, 24 -- Received: from itdsrvhub00.utep.edu (HELO mail.utep.edu) ([129.108.0.69]) 3, 24 -- by itdseciron01.utep.edu with ESMTP/TLS/AES128-SHA; 09 Jun 2011 11:54:32 -0600 3, 24 -- Received: from ITDSRVMBX00.utep.edu ([169.254.1.35]) by ITDSRVHUB00.utep.edu 3, 24 -- ([129.108.0.69]) with mapi; Thu, 9 Jun 2011 11:54:32 -0600 3, 24 -- From: "Arrowood, Roy" {arrowood-at-utep.edu} 3, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 24 -- Date: Thu, 9 Jun 2011 11:54:30 -0600 3, 24 -- Subject: problem with LR White 3, 24 -- Thread-Topic: problem with LR White 3, 24 -- Thread-Index: Acwmzkhfwkn52sNxQveUVcn1InM4Mw== 3, 24 -- Message-ID: {43E96985CDE2184D88C28E130BA692D32538BD27D4-at-ITDSRVMBX00.utep.edu} 3, 24 -- Accept-Language: en-US 3, 24 -- Content-Language: en-US 3, 24 -- X-MS-Has-Attach: 3, 24 -- X-MS-TNEF-Correlator: 3, 24 -- acceptlanguage: en-US 3, 24 -- Content-Type: text/plain; charset="iso-8859-1" 3, 24 -- MIME-Version: 1.0 3, 24 -- Content-Transfer-Encoding: 8bit 3, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p59HsWGa020432 ==============================End of - Headers==============================
Hi Mark, There's so much you haven't told us. Is this a solid state BSE detector, PMT (Robinson) or MCP (micro channel plate)? Is it under mechanical control or computer control? What is your beam voltage? How much beam current are you using? How far is the sample from the detector? What is the atomic # of the sample? What is the sample tilt?
All of these things will affect how much signal is available and it sounds like your basic problem is low signal level, although messing with the dark level or DC off set at high gains can be very touchy. The lines are likely 60Hz interference or possibly the HV oscillator (20kHz-50kHz). More signal will mask them better.
Using 0 tilt, a fairly short WD (3 or 4 mm from the detector), High kV (20kV or above), large spot size (low condenser lens current) and a heavy element sample will all increase your signal level. A diode type detector is the least sensitive and is particularly poor below 10kV. A Robinson or MCP may do better at low voltages. If the detector is computer controlled, it may have a feature that will allow it to set its gain by itself. If it's all manual, be aware that it may be VERY touchy in the highest gain setting.
Give us a little more info.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: piper1960-at-hotmail.com [mailto:piper1960-at-hotmail.com] Sent: Thursday, June 09, 2011 10:02 AM To: kenconverse-at-qualityimages.biz
Hello,
When trying to get images from our BSD,I can get an image in the "high" range (but with faint white lines extending across the screen that rotate off of horizontal at lower scan speeds), but it does not get bright get enough contrast. When it is turned to "very high" range, there are just a series of black and white horizontal lines that zip across the screen.
Any ideas of what to do?
Thanks so much,
Mark
T. Markham Puckett, Ph.D. Associate Professor of Geology Department of Physics and Earth Science P.O. Box 5130 University of North Alabama Florence, Alabama 35632-0001 "Why, sometimes I've believed as many as six impossible things before breakfast." The White Queen to Alice, Through the Looking-Glass
==============================Original Headers============================== 5, 18 -- From piper1960-at-hotmail.com Thu Jun 9 08:54:46 2011 5, 18 -- Received: from col0-omc3-s12.col0.hotmail.com (col0-omc3-s12.col0.hotmail.com [65.55.34.150]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59DiKoE025408 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 08:44:20 -0500 5, 18 -- Received: from COL117-W2 ([65.55.34.137]) by col0-omc3-s12.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 5, 18 -- Thu, 9 Jun 2011 06:42:04 -0700 5, 18 -- Message-ID: {COL117-W29FF2B7898DD4FF598C68BF650-at-phx.gbl} 5, 18 -- X-Originating-IP: [204.29.102.55] 5, 18 -- From: Mark Puckett {piper1960-at-hotmail.com} 5, 18 -- To: {microscopy-at-microscopy.com} 5, 18 -- Subject: BSD problem 5, 18 -- Date: Thu, 9 Jun 2011 08:42:04 -0500 5, 18 -- Importance: Normal 5, 18 -- Content-Type: text/plain; charset="Windows-1252" 5, 18 -- MIME-Version: 1.0 5, 18 -- X-OriginalArrivalTime: 09 Jun 2011 13:42:04.0848 (UTC) FILETIME=[04C98300:01CC26AB] 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p59DiKoE025408 ==============================End of - Headers==============================
==============================Original Headers============================== 20, 28 -- From kenconverse-at-qualityimages.biz Thu Jun 9 14:54:12 2011 20, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 20, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59JsBe8006005 20, 28 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 14:54:11 -0500 20, 28 -- X-Authority-Analysis: v=1.1 cv=pLPlkKcK38cN4Cv4YkVeTGGRYpmKez/IrX640LVKWnc= c=1 sm=0 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=69EAbJreAAAA:8 a=Zx37jsudAAAA:8 a=uWcSPKCLcsbtbVz_HXUA:9 a=0znNXv-9fMVaA0qnqccA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=RyjLG6jaqdoA:10 a=-fXH9i5dZQ0A:10 a=3_2eAsHTqJoA:10 a=EfJqPEOeqlMA:10 a=G91thX30KR8A:10 a=vC7zVQ143SBG9LVm:21 a=_YatpOTCL06tKY2w:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 20, 28 -- X-Cloudmark-Score: 0 20, 28 -- X-Originating-IP: 72.224.149.57 20, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2575] helo=Ken) 20, 28 -- by cdptpa-oedge04.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 20, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 20, 28 -- id ED/F4-20202-2E421FD4; Thu, 09 Jun 2011 19:54:11 +0000 20, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 20, 28 -- To: {piper1960-at-hotmail.com} , "MSA Listserver" {microscopy-at-microscopy.com} 20, 28 -- Subject: RE: [Microscopy] BSD problem 20, 28 -- Date: Thu, 9 Jun 2011 15:54:04 -0400 20, 28 -- Message-ID: {AB6862DDFC664C6BA36858C7EBD62A27-at-Ken} 20, 28 -- MIME-Version: 1.0 20, 28 -- Content-Type: text/plain; 20, 28 -- charset="us-ascii" 20, 28 -- X-Priority: 3 (Normal) 20, 28 -- X-MSMail-Priority: Normal 20, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 20, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6090 20, 28 -- Thread-Index: Acwmrc+JFCoyJCX4RoSYTqQyVkLw3wALtqAg 20, 28 -- Importance: Normal 20, 28 -- In-Reply-To: {201106091402.p59E27mp005080-at-ns.microscopy.com} 20, 28 -- Content-Transfer-Encoding: 8bit 20, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p59JsBe8006005 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jnowak51-at-comcast.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jnowak51-at-comcast.net Name: J. Nowak
Organization: NorthShore University HealthSystem, Evanston, IL
Title-Subject: [Filtered] retired Siemens EM 101 Message: We're about to dispose of (i.e. trash) a Siemens EM 101. I understand this is quite old, but it was still operational not too long ago, and I suspect some others might still be in use. If anyone has any use for this instrument please contact me.
Jan Nowak, PhD, MD Department of Pathology NorthShore University HealthSystem Evanston, IL 60201 jnowak51-at-comcast.net Login Host: 204.26.30.5 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Jun 9 18:38:54 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59NcsAn025078 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 18:38:54 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 2599D3B99D1 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 18:38:54 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id x-krQQvDBKvQ for {microscopy-at-microscopy.com} ; 7, 25 -- Thu, 9 Jun 2011 18:38:53 -0500 (CDT) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id A62963B99C6 7, 25 -- for {microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 18:38:53 -0500 (CDT) 7, 25 -- Message-ID: {4DF1598D.3000409-at-microscopy.com} 7, 25 -- Date: Thu, 09 Jun 2011 18:38:53 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: jnowak51-at-comcast.net 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:retired Siemens EM 101 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sherry.clendenon-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sherry.clendenon-at-gmail.com Name: Sherry G Clendenon
Message: Does anyone have factory service manuals for the Leica TCS SP2 MP system (or even just the Leica TCS SP2)? I've recently started working in a group with an instrument that has been off service contract for some number of years and is not likely to be reinstated in the near future. We need to do some basic maintenance on the system and some minor repairs.
Dear Friends, I am facing the strange TEM alignment problem problem,
I have found the following observations : 1. When we just on the filament, it gives bright beam for a just second, then its intensity become very low. 2. Very low intensity beam is getting on only LM aperture, exactly at 16 degree temp, 1-2 emission step with 1-5 mA emission current. 3. If I increase the magnification higher than LM 490 the beam gets disappear. 4. If we increase or decrease temp. from 16 degree beam getting disappear. 5. If we increase the emission step above 2, beam getting disappear. 6. If we click on alignment, beam getting disappear.
What would be solution for the above mentioned problem. Plz respond as soon as possible.
I had the chance to acquire a Perfect Loop recently, thinking that I can increase the quality of the collected sections. Unfortunately, it was not that much helpful (at least in my hands). I lose plenty of good section when picking them up with the "perfect" loop. The grids that I routinely use are slot grids coated with Formvar, and it is very difficult to transfer from the loop to the grid. I bought tabbed grids in order to handle them better, but then I had to cut the tab, so I had to buy cutter tweezers... All in all, a tool that didn't deserve the investment. But before I leave it in some box and forget it for years, I'd like to hear if there is anybody out there that has used it or still uses it and if there are any special tricks that I can apply. In the meantime, I will still call it the "Imperfect Loop".
Regards, and bon voyage to all the people that are out of their offices!
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS) Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y Dpto. Fisiologia Médica y Biofísica Universidad de Sevilla Facultad de Medicina Avda. Sánchez-Pizjuán 4 41009-Sevilla
I have had excellent results with the Perfect Loop and use it all the time. However, I am not using formvar coated grids, just plain copper mesh.
Geoff
On 6/10/2011 8:53 AM, jmircheski-at-us.es wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I had the chance to acquire a Perfect Loop recently, thinking that I can } increase the quality of the collected sections. Unfortunately, it was not } that much helpful (at least in my hands). I lose plenty of good section when } picking them up with the "perfect" loop. The grids that I routinely use are } slot grids coated with Formvar, and it is very difficult to transfer from } the loop to the grid. I bought tabbed grids in order to handle them better, } but then I had to cut the tab, so I had to buy cutter tweezers... } All in all, a tool that didn't deserve the investment. But before I leave it } in some box and forget it for years, I'd like to hear if there is anybody } out there that has used it or still uses it and if there are any special } tricks that I can apply. In the meantime, I will still call it the } "Imperfect Loop". } } Regards, and bon voyage to all the people that are out of their offices! } } Dr. Josif Mircheski } ____________________________________________________________________________ } ___ } Instituto de Biomedicina de Sevilla (IBIS) } Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y } Dpto. Fisiologia Médica y Biofísica } Universidad de Sevilla } Facultad de Medicina } Avda. Sánchez-Pizjuán 4 } 41009-Sevilla } } Phone:+34-954556103 } Fax:+34-954551769 } e-mail: jmircheski-at-us.es } } } } } ==============================Original Headers============================== } 6, 39 -- From jmircheski-at-us.es Fri Jun 10 07:52:32 2011 } 6, 39 -- Received: from mail.us.es (mail.us.es [193.147.175.20]) } 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5ACqVca001123 } 6, 39 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 07:52:32 -0500 } 6, 39 -- Received: (qmail 18441 invoked from network); 10 Jun 2011 14:52:31 +0200 } 6, 39 -- Received: from unknown (HELO us.es) (192.168.2.13) } 6, 39 -- by us.es with SMTP; 10 Jun 2011 14:52:31 +0200 } 6, 39 -- Received: (qmail 22750 invoked by uid 507); 10 Jun 2011 12:52:28 -0000 } 6, 39 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on antivirus3 } 6, 39 -- X-Spam-Level: * } 6, 39 -- X-Spam-Status: No, score=1.6 required=7.5 tests=BAYES_50,DOS_OUTLOOK_TO_MX, } 6, 39 -- FSL_HELO_NON_FQDN_1,HELO_NO_DOMAIN autolearn=disabled version=3.3.1 } 6, 39 -- Received: from 127.0.0.1 by antivirus3 (envelope-from {jmircheski-at-us.es} , uid 501) with qmail-scanner-2.08 } 6, 39 -- (clamdscan: 0.97/13177. } 6, 39 -- Clear:RC:1(127.0.0.1):. } 6, 39 -- Processed in 0.022841 secs); 10 Jun 2011 12:52:28 -0000 } 6, 39 -- Received: from unknown (HELO antivirus3) (127.0.0.1) } 6, 39 -- by us.es with SMTP; 10 Jun 2011 12:52:28 -0000 } 6, 39 -- Received: from 192.168.1.13 (192.168.1.13) } 6, 39 -- by antivirus3 (F-Secure/fsigk_smtp/403/antivirus3); } 6, 39 -- Fri, 10 Jun 2011 14:52:28 +0200 (CEST) } 6, 39 -- X-Virus-Status: clean(F-Secure/fsigk_smtp/403/antivirus3) } 6, 39 -- Received: (qmail 7978 invoked from network); 10 Jun 2011 14:52:28 +0200 } 6, 39 -- Received: from chacon4.us.es (HELO cubiculos) (jmircheski-at-150.214.11.24) } 6, 39 -- by us.es with RC4-MD5 encrypted SMTP; 10 Jun 2011 14:52:28 +0200 } 6, 39 -- Return-Receipt-To: "Josif Mircheski" {jmircheski-at-us.es} } 6, 39 -- From: "Josif Mircheski" {jmircheski-at-us.es} } 6, 39 -- To: {microscopy-at-microscopy.com} } 6, 39 -- Subject: The Perfect Loop - is it perfect? } 6, 39 -- Date: Fri, 10 Jun 2011 14:52:21 +0200 } 6, 39 -- Message-ID: {!&!AAAAAAAAAAAYAAAAAAAAAEPQoVbwH5VIu5W5KqvqHQfCgAAAEAAAAHH+tZrUnQxGnCnHjD86Ny8BAAAAAA==-at-us.es} } 6, 39 -- MIME-Version: 1.0 } 6, 39 -- Content-Type: text/plain; } 6, 39 -- charset="iso-8859-1" } 6, 39 -- X-Mailer: Microsoft Office Outlook 12.0 } 6, 39 -- thread-index: AcwnbTyHx7tTfPVbQRydnPc6xwJBCQ== } 6, 39 -- Content-Language: en-us } 6, 39 -- Content-Transfer-Encoding: 8bit } 6, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5ACqVca001123 } ==============================End of - Headers============================== } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
==============================Original Headers============================== 10, 63 -- From mcauliff-at-umdnj.edu Fri Jun 10 08:35:59 2011 10, 63 -- Received: from zix01.umdnj.edu (zix01.UMDNJ.EDU [130.219.34.124]) 10, 63 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p5ADZwiM017915 10, 63 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 08:35:59 -0500 10, 63 -- Received: from zix01.umdnj.edu (ZixVPM [127.0.0.1]) 10, 63 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id 9903C1A50EF1 10, 63 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 09:35:58 -0400 (EDT) 10, 63 -- Received: from umdnj.edu (unknown [10.32.15.102]) 10, 63 -- by zix01.umdnj.edu (Proprietary) with ESMTP id 35A0A1A50E48 10, 63 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 09:35:58 -0400 (EDT) 10, 63 -- Received: from ([10.32.15.102]) 10, 63 -- by imail.umdnj.edu with ESMTP with TLS id 8XSJWG1.78244762; 10, 63 -- Fri, 10 Jun 2011 09:35:53 -0400 10, 63 -- Received: from [127.0.0.1] ([10.32.15.102]) 10, 63 -- by scpmmp2.umdnj.edu (Oracle Communications Messaging Exchange Server 10, 63 -- 7u4-21.01 64bit (built Feb 16 2011)) 10, 63 -- with ESMTP id {0LMK00FLBTRSJN70-at-scpmmp2.umdnj.edu} for 10, 63 -- microscopy-at-microscopy.com; Fri, 10 Jun 2011 09:35:53 -0400 (EDT) 10, 63 -- Date: Fri, 10 Jun 2011 09:39:33 -0400 10, 63 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} 10, 63 -- Subject: Re: [Microscopy] The Perfect Loop - is it perfect? 10, 63 -- In-reply-to: {201106101253.p5ACrDB7002423-at-ns.microscopy.com} 10, 63 -- To: jmircheski-at-us.es, microscopy-at-microscopy.com 10, 63 -- Message-id: {4DF21E95.9000806-at-umdnj.edu} 10, 63 -- MIME-version: 1.0 10, 63 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 10, 63 -- References: {201106101253.p5ACrDB7002423-at-ns.microscopy.com} 10, 63 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.13) 10, 63 -- Gecko/20101207 Thunderbird/3.1.7 10, 63 -- X-esp: ESP {10} = 10, 63 -- SHA: {0} 10, 63 -- SHA_FLAGS: {0} 10, 63 -- UHA: {10} 10, 63 -- ISC: {0} 10, 63 -- BAYES: {-1} 10, 63 -- SenderID: {0} 10, 63 -- DKIM: {0} 10, 63 -- TS: {0} 10, 63 -- SIG: {} 10, 63 -- DSC: {0} 10, 63 -- TRU_freehosting: {0} 10, 63 -- TRU_misc_spam: {0} 10, 63 -- TRU_marketing_spam: {0} 10, 63 -- TRU_profanity_spam: {0} 10, 63 -- TRU_spam1: {0} 10, 63 -- TRU_scam_spam: {0} 10, 63 -- TRU_money_spam: {0} 10, 63 -- URL Real-Time Signatures: {0} 10, 63 -- TRU_watch_spam: {0} 10, 63 -- TRU_embedded_image_spam: {1} 10, 63 -- TRU_spam2: {0} 10, 63 -- TRU_medical_spam: {0} 10, 63 -- TRU_playsites: {0} 10, 63 -- TRU_html_image_spam: {0} 10, 63 -- TRU_stock_spam: {0} 10, 63 -- TRU_urllinks: {0} 10, 63 -- TRU_adult_spam: {0} 10, 63 -- TRU_legal_spam: {0} 10, 63 -- TRU_lotto_spam: {0} 10, 63 -- TRU_ru_spamsubj: {0} 10, 63 -- TRU_phish_spam: {0} 10, 63 -- Content-Transfer-Encoding: 8bit 10, 63 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id p5ADZwiM017915 ==============================End of - Headers==============================
Here is a how I use it: after cutting the ultra sections, I separate them (by ones, twos, threes etc.) and then lower the loop on top of them, avoiding that the rim of the loop touches the sections. Then, I raise the loop, trying to minimize the water droplet taken by the loop by raising the loop near the edge of the boat. Then, while holding the grid with tweezers (grid is single slot, formvar coated), I touch the loop on the grid surface, trying to match the orientation of the section with the orientation of the slot so that it sticks to the film only, but not on the metal. The tricky part is there - the presence of the tweezers' tips prevents that the loop and the grid stick perfectly and to easily transfer the section. This is the point where I lose most of the sections. I used the tabbed slot grids in order to remove the tip away from the contact area, but then I still have problems while picking up the sections and transferring them with the loop at the grid. Also, the tabs need to be removed, so I have to put three more steps in the handling of the grid - I lose grids there, too. So, I guess this explanation can help somebody to tell me what I do wrong, or where I can improve. And, I have been trying various modes, for months now...
Thanks again and regards,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS) Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y Dpto. Fisiologia Médica y Biofísica Universidad de Sevilla Facultad de Medicina Avda. Sánchez-Pizjuán 4 41009-Sevilla
-----Original Message----- X-from: Ralph Common [mailto:common-at-msu.edu] Sent: Friday, June 10, 2011 3:10 PM To: jmircheski-at-us.es
Josif,
I also use the perfect-loop for naked grids and it works perfectly. It is most useful when ribbons are not achieved and sections are all individual.
I tried it with coated grids for a short while and had trouble like you described. I did not hold the grid but had it sitting on a piece of filter paper. I got the best results by first filling the open loop with as many sections as would fit. That way when they moved with the water being wicked out there were still sections covering the slot of the grid.
Getting the final bit of water out between the grid and the loop was where I had the most trouble. I finally gave up as it was easier to take the time to trim better and make ribbons of sections. I pick them up the old fashioned way of dipping the grid into the water of the boat, hooking the top of the ribbon over the edge of the grid and lifting the grid up at a fairly steep angle so that the rest of the ribbon lays nicely across the slot of the grid. Newly coated grids or ones that have been glow discharged are needed to do this for older ones tend to be hydrophobic and the sections will "run away".
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
The best technique I've tried in getting sections onto a coated slotted grid with consistent results is to use homemade support films (e.g. formvar or butvar) and a domino rack (EMS# 70620). The domino rack is a great investment. I use it everytime I transfer serial sections on coated slotted grids.
After casting the film on water surface, the domino rack is carefully used to pick up the film. The film on the rack is allowed to dry. Sections are picked up with the slotted grid (The grid serves now as your loop). The slotted grid containing the sections suspended in water is carefully placed on the film on the rack and allowed to dry.
This technique is carefully described in Biological Specimen Preparation for Correlative Light and Electron Microscopy by David Moran and Carter Rowley.
After reading your post, I wanted to start looking for my commercial perfect loop. It's been missing for a while but I don't think I need it for now.
Regards,
Claire
==============================Original Headers============================== 7, 30 -- From chaueter-at-bcm.edu Fri Jun 10 12:39:16 2011 7, 30 -- Received: from iron2.corp.bcm.tmc.edu (iron2.corp.bcm.tmc.edu [128.249.224.14]) 7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5AHdFYa004685 7, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 12:39:16 -0500 7, 30 -- X-IronPort-AV: E=Sophos;i="4.65,348,1304312400"; 7, 30 -- d="scan'208";a="97392063" 7, 30 -- Received: from unknown (HELO EPW-EXHUB3-P022.ad.bcm.edu) ([10.22.132.49]) 7, 30 -- by iron2-out.bcm.edu with ESMTP; 10 Jun 2011 12:39:15 -0500 7, 30 -- Received: from EXCMSMBX03.ad.bcm.edu ([fe80::a5f2:b0d8:7a66:1bbf]) by 7, 30 -- EPW-EXHUB3-P022.ad.bcm.edu ([::1]) with mapi; Fri, 10 Jun 2011 12:39:15 -0500 7, 30 -- From: "Haueter, Claire Menoza" {chaueter-at-bcm.edu} 7, 30 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 30 -- Date: Fri, 10 Jun 2011 12:39:14 -0500 7, 30 -- Subject: [Microscopy] Re: The Perfect Loop - is it perfect? 7, 30 -- Thread-Topic: [Microscopy] Re: The Perfect Loop - is it perfect? 7, 30 -- Thread-Index: AQHMJ5VRFpaHIPu7RUegf18Z8s7bZQ== 7, 30 -- Message-ID: {F3EEF3767DFF3244826E72285C1C27344AE6BE1C5E-at-EXCMSMBX03.ad.bcm.edu} 7, 30 -- Accept-Language: en-US 7, 30 -- Content-Language: en-US 7, 30 -- X-MS-Has-Attach: 7, 30 -- X-MS-TNEF-Correlator: 7, 30 -- acceptlanguage: en-US 7, 30 -- x-tm-as-product-ver: SMEX-8.0.0.4194-6.500.1024-18192.000 7, 30 -- x-tm-as-result: No--29.552900-8.000000-31 7, 30 -- x-tm-as-user-approved-sender: No 7, 30 -- x-tm-as-user-blocked-sender: No 7, 30 -- Content-Type: text/plain; charset="us-ascii" 7, 30 -- MIME-Version: 1.0 7, 30 -- Content-Transfer-Encoding: 8bit 7, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5AHdFYa004685 ==============================End of - Headers==============================
You don't say if this a FEG of W/LaB6 source but, assuming a W/LaB6 source, the first thing I would do is remove the wehnelt and check the physical alignment of the filament in the wehnelt and that the whole filament assembly is securely fastened in position.
My first guess at what is happening is that a physical misalignment of the filament means the electrons are coming out of the wehnelt at an odd angle. As the filament heating is adjusted, the angle changes (or the filament moves).
Larry
On 10 Jun 2011, at 13:26, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Friends, } I am facing the strange TEM alignment problem problem, } } I have found the following observations : } 1. When we just on the filament, it gives bright beam for a just } second, then its intensity become very low. } 2. Very low intensity beam is getting on only LM aperture, exactly at 16 } degree temp, 1-2 emission step with 1-5 mA emission current. } 3. If I increase the magnification higher than LM 490 the beam gets } disappear. } 4. If we increase or decrease temp. from 16 degree beam getting disappear. } 5. If we increase the emission step above 2, beam getting disappear. } 6. If we click on alignment, beam getting disappear. } } What would be solution for the above mentioned problem. } Plz respond as soon as possible. } } } ------------------------------------------------------------------------- } Ravindra Thakkar } Cryo TEM - Central Facility, } 309 - CRNTS, } I.I.T. Bombay, } Mumbai - 400076 (INDIA) } Tel. : +91-22-25764688/4681 } Mo. : +91-9769474636 } E-mail : ravi.thakkar-at-iitb.ac.in } ------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 6, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Jun 10 07:20:48 2011 } 6, 25 -- Received: from znl.com ([206.69.208.20]) } 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5ACKm6k016938 } 6, 25 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 07:20:48 -0500 } 6, 25 -- Received: from localhost (localhost [127.0.0.1]) } 6, 25 -- by znl.com (Postfix) with ESMTP id 623C33BA0F3 } 6, 25 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 07:20:47 -0500 (CDT) } 6, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 6, 25 -- Received: from znl.com ([127.0.0.1]) } 6, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 6, 25 -- with ESMTP id FDgJnkrR+QGd for {microscopy-at-microscopy.com} ; } 6, 25 -- Fri, 10 Jun 2011 07:20:46 -0500 (CDT) } 6, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 6, 25 -- by znl.com (Postfix) with ESMTPA id A57FC3BA0E8 } 6, 25 -- for {microscopy-at-microscopy.com} ; Fri, 10 Jun 2011 07:20:46 -0500 (CDT) } 6, 25 -- Message-ID: {4DF20C1E.6070507-at-microscopy.com} } 6, 25 -- Date: Fri, 10 Jun 2011 07:20:46 -0500 } 6, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 6, 25 -- Reply-To: Ravi Thakkar {ravi.thakkar369-at-gmail.com} } 6, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 6, 25 -- MIME-Version: 1.0 } 6, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 6, 25 -- Subject: viaWWW: TEM : Electron Beam is not getting proper URGENT } 6, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 6, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
Larry Stoter (Working on a Microsoft-free computer)
==============================Original Headers============================== 10, 22 -- From larry-at-cymru666.plus.com Sat Jun 11 00:08:15 2011 10, 22 -- Received: from relay.pcl-ipout02.plus.net (relay.pcl-ipout02.plus.net [212.159.7.100]) 10, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5B58EwA029009 10, 22 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Jun 2011 00:08:15 -0500 10, 22 -- X-IronPort-Anti-Spam-Filtered: true 10, 22 -- X-IronPort-Anti-Spam-Result: Av8EAJL38k3Unw4T/2dsb2JhbABTEKVLcnfGK4YkBJYAhD+GFTY 10, 22 -- Received: from outmx04.plus.net ([212.159.14.19]) 10, 22 -- by relay.pcl-ipout02.plus.net with ESMTP; 11 Jun 2011 06:08:13 +0100 10, 22 -- Received: from [46.208.80.219] (helo=[10.175.1.101]) 10, 22 -- by outmx04.plus.net with esmtp (Exim) id 1QVGQf-00027G-GU; Sat, 11 Jun 2011 06:08:13 +0100 10, 22 -- Content-Type: text/plain; charset=us-ascii 10, 22 -- Mime-Version: 1.0 (Apple Message framework v1084) 10, 22 -- Subject: Re: [Microscopy] viaWWW: TEM : Electron Beam is not getting proper URGENT 10, 22 -- From: Larry Stoter {larry-at-cymru666.plus.com} 10, 22 -- In-Reply-To: {201106101226.p5ACQtLm025431-at-ns.microscopy.com} 10, 22 -- Date: Sat, 11 Jun 2011 06:08:12 +0100 10, 22 -- Message-Id: {E8389308-A9E1-47F4-AC69-24B389233860-at-cymru666.plus.com} 10, 22 -- References: {201106101226.p5ACQtLm025431-at-ns.microscopy.com} 10, 22 -- To: ravi.thakkar-at-iitb.ac.in, MSA Microscopy List {Microscopy-at-microscopy.com} 10, 22 -- X-Mailer: Apple Mail (2.1084) 10, 22 -- Content-Transfer-Encoding: 8bit 10, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5B58EwA029009 ==============================End of - Headers==============================
Senior Technician/Technician - SEM AUT University School of Engineering - SEM (Scanning Electron Microscope) Technician
The School of Engineering is undertaking exciting research in advanced materials and manufacturing processes. The school is currently installing a state-of-the-art SEM facility. This will support microstructure research in advanced materials and metallurgy but also in biosciences. Particular areas of materials research interest are; the solidification related processes of casting and welding, plastic deformation processes of machining, friction stir welding, extrusion, rolling, forging and sheet metal forming, and microelectronic materials and devices.
Our new Hitachi SU-70 SEM system will be entering service after mid-year 2011. We are looking for an appropriately qualified Technician to help establish and support all aspects of our SEM/EBSD facility.
The role will also involve operating materials test and measurement equipment and support of engineering instrumentation and data acquisition systems used in laboratory classes for Engineering degree students and in postgraduate research.
Do you have the skills to operate and manage an SEM facility, and carry out wider technical laboratory duties? If so, then this job may be for you.
Tertiary education is a diverse and rewarding work environment. You will be working with a wide range of students, staff and external clients, varied requirements and unusual teaching and research projects. We are looking for someone motivated and versatile who can make a difference.
Ref: 10941
Closing Date: 22 June 2011
For more information please see: http://careers.aut.ac.nz/jobdetails?ajid=6Y4B7
Dave Crofts Technical Services Manager | School of Engineering | AUT University | phone: 09 921 9999 ext 8154 | mobile: 021 339 581 | fax: 09 921 9973 | email: dave.crofts-at-aut.ac.nz
==============================Original Headers============================== 16, 26 -- From dave.crofts-at-aut.ac.nz Sun Jun 12 20:33:34 2011 16, 26 -- Received: from horuhoru.aut.ac.nz (horuhoru-3.aut.ac.nz [156.62.3.13]) 16, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5D1XWqP012763 16, 26 -- for {microscopy-at-microscopy.com} ; Sun, 12 Jun 2011 20:33:33 -0500 16, 26 -- Received: (from root-at-localhost) 16, 26 -- by horuhoru.aut.ac.nz (8.14.2/8.14.2) id p5D1XT53012183 16, 26 -- for microscopy-at-microscopy.com; Mon, 13 Jun 2011 13:33:29 +1200 16, 26 -- Received: from localhost(156.62.1.10) by horuhoru.aut.ac.nz via smap (V2.0) 16, 26 -- id xma012052; Mon, 13 Jun 11 13:33:12 +1200 16, 26 -- Received: from eden.aut.ac.nz ([156.62.1.10] helo=eden.aut.ac.nz) by 16, 26 -- horuhoru.aut.ac.nz with SMTP (ASSP 1.8.5.9); 13 Jun 2011 13:33:12 +1200 16, 26 -- Received: from smtpdom.aut.ac.nz (smtpdom.aut.ac.nz [156.62.50.184]) 16, 26 -- by eden.aut.ac.nz (8.14.3/8.14.3) with ESMTP id p5D1X9Kd024609 16, 26 -- for {Microscopy-at-Microscopy.Com} ; Mon, 13 Jun 2011 13:33:10 +1200 16, 26 -- Received: from SMTPDOM-MTA by smtpdom.aut.ac.nz 16, 26 -- with Novell_GroupWise; Mon, 13 Jun 2011 13:33:09 +1200 16, 26 -- Message-Id: {4DF61194.FCFC.0065.1-at-aut.ac.nz} 16, 26 -- Date: Mon, 13 Jun 2011 13:33:08 +1200 16, 26 -- From: "Dave Crofts" {dave.crofts-at-aut.ac.nz} 16, 26 -- To: {Microscopy-at-microscopy.com} 16, 26 -- Subject: SEM Technician Vacancy - Auckland, New Zealand 16, 26 -- Mime-Version: 1.0 16, 26 -- Content-Type: text/plain; charset=US-ASCII 16, 26 -- Content-Disposition: inline 16, 26 -- Content-Transfer-Encoding: 8bit 16, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5D1XWqP012763 ==============================End of - Headers==============================
During my last semester of studying electron microscopy at San Joaquin Delta College, I used a Gatan 693 Ilion Advantage planar polishing system to prepare the transverse of different hair samples for SEM (canine, feline, human).
The Ilion uses dual ion beams to mill/polish the surface of a cross section, plus a cold stage for heat sensitive samples. The porous structures in the cortex and medulla were nicely preserved with no distortions. The hairs were not embedded but sandwiched between two 1.7 mm glass cover slips and held in place with double-stick tape and gel superglue.
If fracturing does not give you the desired results, you may want to check with Gatan to see if they can either prepare the sample for you, or point you to someone in your area who has an Ilion. You can e-mail me off-line if you need a contact at Gatan.
Gigi Kemalyan Research Technician Nogales Lab, HHMI at UC Berkeley singinggardenersx2-at-live.com
==============================Original Headers============================== 7, 18 -- From singinggardenersx2-at-live.com Sun Jun 12 21:52:10 2011 7, 18 -- Received: from col0-omc3-s16.col0.hotmail.com (col0-omc3-s16.col0.hotmail.com [65.55.34.154]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5D2qAUD029854 7, 18 -- for {microscopy-at-microscopy.com} ; Sun, 12 Jun 2011 21:52:10 -0500 7, 18 -- Received: from COL115-W63 ([65.55.34.137]) by col0-omc3-s16.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 7, 18 -- Sun, 12 Jun 2011 19:52:09 -0700 7, 18 -- Message-ID: {COL115-W635C97C2338989BCAFA53EE5690-at-phx.gbl} 7, 18 -- X-Originating-IP: [69.226.98.51] 7, 18 -- From: Gigi K {singinggardenersx2-at-live.com} 7, 18 -- To: {microscopy-at-microscopy.com} , {yun.peng-at-ge.com} 7, 18 -- Subject: Re: About cross section study by SEM 7, 18 -- Date: Sun, 12 Jun 2011 19:52:09 -0700 7, 18 -- Importance: Normal 7, 18 -- Content-Type: text/plain; charset="iso-8859-1" 7, 18 -- MIME-Version: 1.0 7, 18 -- X-OriginalArrivalTime: 13 Jun 2011 02:52:09.0744 (UTC) FILETIME=[E38BD900:01CC2974] 7, 18 -- Content-Transfer-Encoding: 8bit 7, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5D2qAUD029854 ==============================End of - Headers==============================
Thank you for sharing your experience with me about the Perfect Loop. From your answers I can summarize that it is better to use it with normal grids, and not with slotted grids. I use only slot grids (for now), so I guess it won’t be of much use in the near future. I guess I should have asked this question before buying it. Nevertheless, now I know that it can help me in other applications. Thanks again.
Regards,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
X-from: Gnägi Helmut [mailto:helmut.gnaegi-at-diatome.ch] Sent: Saturday, June 11, 2011 3:52 PM To: 'jmircheski-at-us.es' Cc: 'sgkcck-at-aol.com'
I had the chance to acquire a Perfect Loop recently, thinking that I can increase the quality of the collected sections. Unfortunately, it was not that much helpful (at least in my hands). I lose plenty of good section when picking them up with the "perfect" loop. The grids that I routinely use are slot grids coated with Formvar, and it is very difficult to transfer from the loop to the grid. I bought tabbed grids in order to handle them better, but then I had to cut the tab, so I had to buy cutter tweezers... All in all, a tool that didn't deserve the investment. But before I leave it in some box and forget it for years, I'd like to hear if there is anybody out there that has used it or still uses it and if there are any special tricks that I can apply. In the meantime, I will still call it the "Imperfect Loop".
Regards, and bon voyage to all the people that are out of their offices!
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS) Hosp.Univ. Virgen del Rocío/CSIC/Univ. de Sevilla y Dpto. Fisiologia Médica y Biofísica Universidad de Sevilla Facultad de Medicina Avda. Sánchez-Pizjuán 4 41009-Sevilla
I have a student who wants to microtome some materials samples. The samples are GaSb and Si wafers. The length and the width are less than 1cm each. The thickness, which is the most important is less than 0.5mm for GaSb and 1mm for Si. The structures are on top of the support surfaces (same material). The GaSb samples are really not very hard.
My concern is that these are students who have never touched a microtome. They need advise as to type of microtome knife to purchase and any other hints you have for success with these samples. Will it be necessary to embed them in resin (which one?) to stabilize the samples in the chuck? Most likely the resin will peel away from the cut sections but that is not a problem.
I will teach them using a resin block and glass knives so that they get the idea but that is a long way from success with their samples. They are hoping for thicknesses of 100-300nm and will be imaging them with a 300kV FEI Titan TEM. FIB is not an option with these samples as the ion beam destroys the detail of interest.
Thanks to all in advance, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 11, 28 -- From dsherman-at-purdue.edu Mon Jun 13 19:55:37 2011 11, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5E0tZvw016035 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jun 2011 19:55:37 -0500 11, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 11, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5E0tWUQ029756 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jun 2011 20:55:32 -0400 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.138]) by WPPEXHUB02F.purdue.lcl 11, 28 -- ([::1]) with mapi; Mon, 13 Jun 2011 20:55:32 -0400 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 28 -- Date: Mon, 13 Jun 2011 20:55:30 -0400 11, 28 -- Subject: Microtoming materials samples 11, 28 -- Thread-Topic: Microtoming materials samples 11, 28 -- Thread-Index: AcwqLcHJ03GrcWKL/06qhgIlD+rLUA== 11, 28 -- Message-ID: {CA1C29C2.F0F2%dsherman-at-purdue.edu} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="us-ascii" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-PMX-Version: 5.5.9.388399 11, 28 -- X-PerlMx-Virus-Scanned: Yes 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5E0tZvw016035 ==============================End of - Headers==============================
Debby, It has been done with semiconductors, but the results give you a bunch of cleaved stuff that doesn't look great.
If they are looking for a relatively straightforward process for making TEM samples that doesn't require very expensive equipment, I highly recommend the MicroCleave(TM) technique, also known as the Small Angle Cleavage Technique. This technique is fairly easy to learn and it will make absolutely fantastic samples with these types of materials. Here are several links to application notes on our website. We also have a video on how to do the technique that we can send out.
The first of these files have references for the technique.
If you like, have your students give me a call and I will talk to them about the technique. It is a much better approach for these materials than microtoming. For about the cost of a knife (or less), they could buy the equipment and supplies to do it or they could purchase our MicroCleave(TM) kit.
Disclaimer: South Bay Technology manufactures and sells the MicroCleave(TM) Kit Model 520.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Monday, June 13, 2011 6:04 PM To: swalck-at-southbaytech.com
Hi all,
I have a student who wants to microtome some materials samples. The samples are GaSb and Si wafers. The length and the width are less than 1cm each. The thickness, which is the most important is less than 0.5mm for GaSb and 1mm for Si. The structures are on top of the support surfaces (same material). The GaSb samples are really not very hard.
My concern is that these are students who have never touched a microtome. They need advise as to type of microtome knife to purchase and any other hints you have for success with these samples. Will it be necessary to embed them in resin (which one?) to stabilize the samples in the chuck? Most likely the resin will peel away from the cut sections but that is not a problem.
I will teach them using a resin block and glass knives so that they get the idea but that is a long way from success with their samples. They are hoping for thicknesses of 100-300nm and will be imaging them with a 300kV FEI Titan TEM. FIB is not an option with these samples as the ion beam destroys the detail of interest.
Thanks to all in advance, Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 11, 28 -- From dsherman-at-purdue.edu Mon Jun 13 19:55:37 2011 11, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5E0tZvw016035 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jun 2011 19:55:37 -0500 11, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 11, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5E0tWUQ029756 11, 28 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jun 2011 20:55:32 -0400 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.138]) by WPPEXHUB02F.purdue.lcl 11, 28 -- ([::1]) with mapi; Mon, 13 Jun 2011 20:55:32 -0400 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 28 -- Date: Mon, 13 Jun 2011 20:55:30 -0400 11, 28 -- Subject: Microtoming materials samples 11, 28 -- Thread-Topic: Microtoming materials samples 11, 28 -- Thread-Index: AcwqLcHJ03GrcWKL/06qhgIlD+rLUA== 11, 28 -- Message-ID: {CA1C29C2.F0F2%dsherman-at-purdue.edu} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="us-ascii" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-PMX-Version: 5.5.9.388399 11, 28 -- X-PerlMx-Virus-Scanned: Yes 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5E0tZvw016035 ==============================End of - Headers==============================
==============================Original Headers============================== 36, 23 -- From swalck-at-southbaytech.com Mon Jun 13 20:52:28 2011 36, 23 -- Received: from relay03.pair.com (relay03.pair.com [209.68.5.17]) 36, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p5E1qSWn004983 36, 23 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jun 2011 20:52:28 -0500 36, 23 -- Received: (qmail 27826 invoked from network); 14 Jun 2011 01:52:27 -0000 36, 23 -- Received: from 99.154.21.202 (HELO swalckPC) (99.154.21.202) 36, 23 -- by relay03.pair.com with SMTP; 14 Jun 2011 01:52:26 -0000 36, 23 -- X-pair-Authenticated: 99.154.21.202 36, 23 -- From: "Scott Walck" {swalck-at-southbaytech.com} 36, 23 -- To: {microscopy-at-microscopy.com} 36, 23 -- Cc: {dsherman-at-purdue.edu} 36, 23 -- References: {201106140103.p5E13bKl027623-at-ns.microscopy.com} 36, 23 -- In-Reply-To: {201106140103.p5E13bKl027623-at-ns.microscopy.com} 36, 23 -- Subject: RE: [Microscopy] Microtoming materials samples 36, 23 -- Date: Mon, 13 Jun 2011 18:57:00 -0700 36, 23 -- Message-ID: {006601cc2a36$5d35cfb0$17a16f10$-at-com} 36, 23 -- MIME-Version: 1.0 36, 23 -- Content-Type: text/plain; 36, 23 -- charset="us-ascii" 36, 23 -- Content-Transfer-Encoding: 7bit 36, 23 -- X-Mailer: Microsoft Office Outlook 12.0 36, 23 -- Thread-Index: AcwqLuSoXmAsVJy/RUOBVxJTcNQ4OQABAWWg 36, 23 -- Content-Language: en-us ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cvierret-at-mst.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cvierret-at-mst.edu Name: Clarissa Wisner
Organization: MS&T
Title-Subject: [Filtered] JEOL 733 Microprobe
Message: We are surplusing our JEOL 733 microprobe. It will sold as it sets and the buyer will have to arrange shipping. If you want more information please let me know privately and I will be happy to assist you.
If you know of anyone that might need or want this instrument please send this along.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jacques.werckmann-at-ipcms.u-strasbg.fr as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jacques.werckmann-at-ipcms.u-strasbg.fr Name: jacques Werckmann
Organization: UFRJ
Title-Subject: [Filtered] STEM
Message: I study, with a STEM, LaAlO3. zone axis (001). On a Jeol 2100F (200kV) uncorrected I see the columns of LaO and AlO those by cons on a Titan corrected (300kV) I do not see the columns of AlO. A microscopist would he explain this difference Thanks Jacques
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both parishcm-at-ornl.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: parishcm-at-ornl.gov Name: Chad Parish
Organization: ORNL
Title-Subject: [Filtered] EBSD: sample prep for cordierite Mg-Al-Si-oxides
Message: Dear listers,
I'm more of a metallurgist, but I've had a customer ask for EBSD of Cordierite (Mg2Al4Si5O18). We tried a colloidal silica polish, and as I had hypothesized, we did not get usable patterns. I've ordered a few sample preparation articles suggested in the Prior et al. chapter of the recent EBSD book, but while I'm waiting for them to arrive, can anyone suggest some references or recipes that might help me out?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alice.dohnalkova-at-pnnl.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: alice.dohnalkova-at-pnnl.gov Name: Alice Dohnalkova
Message: Dear list, Here is a question from my colleague:
An older, yet never opened bottle of nanoplast catalyst have some hardened, crunchy-looking crystalline material inside. Is there any way how to reconstitute that?
Recently I got a Edwards S150 carbon coating unit (Part Nr E087-23-003), the unit alone without the vaccum vessel, i.e. the power supply with two cables fixed to an Al flange, with some sort of holder for the carbon rods on it. As it look like, and compared to a picture found on internet (on an auction web site...), I fear that my head is not complete.
So I'm looking for a picture, or a sketch, of a garanted working head, and if possible for the a copy of the manual (paper or pdf file).
Does someone on the list have this stuff ?
Many thanks in advance
-- J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Our lab recently acquired a used Sorvall MT-5000 microtome. It works well and I would like to get a manual with instructions and other technical info. Does anybody know who bought Sorvall in recent years? I was informed that RMC bought it a while ago and then passed it on to another company... Thanks a lot!!
-- Tami Bogea PhD Research Associate Ophthalmology & Visual Sciences University of British Columbia 2550 Willow St. Vancouver, BC V5Z 3N9 Canada
==============================Original Headers============================== 4, 25 -- From tbogea-at-interchange.ubc.ca Wed Jun 15 18:48:41 2011 4, 25 -- Received: from vmaprod1.mail-relay.ubc.ca (vmaprod1.mail-relay.ubc.ca [142.103.117.132]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5FNmfm6012182 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 18:48:41 -0500 4, 25 -- Received: from vmaprod1.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 4, 25 -- by localhost (Email Security Appliance) with SMTP id B667129E7BA_DF944D8B 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 23:48:40 +0000 (GMT) 4, 25 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) 4, 25 -- by vmaprod1.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTP id 8A54729E7A0_DF944D8F 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 23:48:40 +0000 (GMT) 4, 25 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12]) 4, 25 -- by smtp.interchange.ubc.ca 4, 25 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) 4, 25 -- with ESMTP id {0LMU001D6VH387-at-smtp.interchange.ubc.ca} for 4, 25 -- Microscopy-at-microscopy.com; Wed, 15 Jun 2011 16:48:40 -0700 (PDT) 4, 25 -- Date: Wed, 15 Jun 2011 16:48:39 -0700 (PDT) 4, 25 -- From: Tami Bogea {tbogea-at-interchange.ubc.ca} 4, 25 -- Subject: Sorvall MT-5000 microtome 4, 25 -- To: Microscopy-at-microscopy.com 4, 25 -- Message-id: {4295219.6001308181719468.JavaMail.myubc2-at-brahms.my.ubc.ca} 4, 25 -- MIME-version: 1.0 4, 25 -- X-Mailer: uPortal WEB email client 3.0 4, 25 -- Content-type: text/plain; charset=us-ascii 4, 25 -- Content-transfer-encoding: 7bit 4, 25 -- X-UBC-Relayed: Relayed through vma.mail-relay.ubc.ca ==============================End of - Headers==============================
I am trying to acquire HOLZ lines in 000 transmitted spot of CBED patterns for point group determination purpose, but have been experiencing difficulties in acquiring/recording images of HOLZ lines. The TEM I am using is Philips EM-20 with Gatan camera. On the phosphor screen, I was able to see HOLZ lines for higher index zone axis patterns and they seems to be clearer under 80kV than 200kV. However, when I inserted the Gatan camera to take pictures, I noticed that the quality of the diffraction images was too bad - the HOLZ lines were blurry, and some areas were too bright, making the nearby HOLZ lines invisible. Does anyone on this list have similar problem before? Any suggestions on how to record sharp/clear HOLZ images would be much appreciated!
Best regards,
Chuan Zhang Graduate Student Researcher, Dept. of Chemical Engineering and Materials Science, University of California, Irvine
==============================Original Headers============================== 4, 33 -- From zhangchuan827-at-gmail.com Thu Jun 16 01:52:57 2011 4, 33 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G6qurU001454 4, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:52:57 -0500 4, 33 -- Received: by vxk20 with SMTP id 20so1186653vxk.0 4, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 23:52:56 -0700 (PDT) 4, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 33 -- d=gmail.com; s=gamma; 4, 33 -- h=domainkey-signature:mime-version:sender:from:date 4, 33 -- :x-google-sender-auth:message-id:subject:to:content-type; 4, 33 -- bh=3BCUubw8UQvFaIuaHjXqwmLSxm5++INmIemWH5pYPSU=; 4, 33 -- b=YGaDpTcRnzV1eeI9BbQ3b6SJTW+gF13/ox5hrM4Fz9xttahrmh2qUVAbLnS2w/+Hhe 4, 33 -- 3wcfAEJnSwatogkn8/UYx85qkmJ5FJ942Y9zfiPpOkfnQHNMOCWn652tNuuFxedj70yh 4, 33 -- beEiCB5Qq49d1o8vXW9/HUD+DAtqN3mtBpzTI= 4, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 33 -- d=gmail.com; s=gamma; 4, 33 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 4, 33 -- :subject:to:content-type; 4, 33 -- b=Kf16Bnn/c9C9N+fqLfrgd3f1qYT7bJcKnnVZpIXk5KkByvAK6uJw/OcecVMYeSmCIz 4, 33 -- e1anKyVhbci0T86boI5wWmnMJJN8m1QX548MKW9t57MxdH/m+M/R+L60GyLugmCFHRcJ 4, 33 -- 4u+sGiSXMtry6kHcVCXMq7apyx9V5fygICwHU= 4, 33 -- Received: by 10.52.187.164 with SMTP id ft4mr734594vdc.59.1308207176102; Wed, 4, 33 -- 15 Jun 2011 23:52:56 -0700 (PDT) 4, 33 -- MIME-Version: 1.0 4, 33 -- Sender: zhangchuan827-at-gmail.com 4, 33 -- Received: by 10.52.113.35 with HTTP; Wed, 15 Jun 2011 23:52:16 -0700 (PDT) 4, 33 -- From: Chuan Zhang {chuanz-at-uci.edu} 4, 33 -- Date: Wed, 15 Jun 2011 23:52:16 -0700 4, 33 -- X-Google-Sender-Auth: f54XRRGmX0SHm0dNdP4cGNdXWRg 4, 33 -- Message-ID: {BANLkTinLay6CrYhPqCDEPqtSk6ir4z4OkQ-at-mail.gmail.com} 4, 33 -- Subject: TEM - Problem with Acquiring HOLZ lines in 000 spot 4, 33 -- To: Microscopy-at-microscopy.com 4, 33 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Hi Tami, I am doing service for RMC here in Germany. I will send you a PDF of the service manual offline. I do not have a manual for the MT-5000 but will include an operation mannual of the MT-7000 which is mostly similar in functions like the 5000...
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 16.06.11 01:52, schrieb tbogea-at-interchange.ubc.ca: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all! } } Our lab recently acquired a used Sorvall MT-5000 microtome. It works well and I would like to get a manual with instructions and other technical info. Does anybody know who bought Sorvall in recent years? I was informed that RMC bought it a while ago and then passed it on to another company... Thanks a lot!! } } -- } Tami Bogea PhD } Research Associate } Ophthalmology& Visual Sciences } University of British Columbia } 2550 Willow St. Vancouver, BC } V5Z 3N9 Canada } } } ==============================Original Headers============================== } 4, 25 -- From tbogea-at-interchange.ubc.ca Wed Jun 15 18:48:41 2011 } 4, 25 -- Received: from vmaprod1.mail-relay.ubc.ca (vmaprod1.mail-relay.ubc.ca [142.103.117.132]) } 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5FNmfm6012182 } 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 18:48:41 -0500 } 4, 25 -- Received: from vmaprod1.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) } 4, 25 -- by localhost (Email Security Appliance) with SMTP id B667129E7BA_DF944D8B } 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 23:48:40 +0000 (GMT) } 4, 25 -- Received: from mta1.interchange.ubc.ca (mta1.interchange.ubc.ca [142.103.145.69]) } 4, 25 -- by vmaprod1.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTP id 8A54729E7A0_DF944D8F } 4, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 23:48:40 +0000 (GMT) } 4, 25 -- Received: from brahms.my.ubc.ca (brahms.my.ubc.ca [137.82.115.12]) } 4, 25 -- by smtp.interchange.ubc.ca } 4, 25 -- (iPlanet Messaging Server 5.2 HotFix 1.21 (built Sep 8 2003)) } 4, 25 -- with ESMTP id {0LMU001D6VH387-at-smtp.interchange.ubc.ca} for } 4, 25 -- Microscopy-at-microscopy.com; Wed, 15 Jun 2011 16:48:40 -0700 (PDT) } 4, 25 -- Date: Wed, 15 Jun 2011 16:48:39 -0700 (PDT) } 4, 25 -- From: Tami Bogea {tbogea-at-interchange.ubc.ca} } 4, 25 -- Subject: Sorvall MT-5000 microtome } 4, 25 -- To: Microscopy-at-microscopy.com } 4, 25 -- Message-id: {4295219.6001308181719468.JavaMail.myubc2-at-brahms.my.ubc.ca} } 4, 25 -- MIME-version: 1.0 } 4, 25 -- X-Mailer: uPortal WEB email client 3.0 } 4, 25 -- Content-type: text/plain; charset=us-ascii } 4, 25 -- Content-transfer-encoding: 7bit } 4, 25 -- X-UBC-Relayed: Relayed through vma.mail-relay.ubc.ca } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 22 -- From stefan.diller-at-t-online.de Thu Jun 16 02:16:40 2011 5, 22 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G7Gdhg017881 5, 22 -- for {microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 02:16:40 -0500 5, 22 -- Received: from fwd14.aul.t-online.de (fwd14.aul.t-online.de ) 5, 22 -- by mailout04.t-online.de with smtp 5, 22 -- id 1QX6og-0004dA-Ol; Thu, 16 Jun 2011 09:16:38 +0200 5, 22 -- Received: from mac-pro.speedport.ip (VmlGNiZv8hB76Xez5EbVQ+OY0Tm-Jj6-+dvcGXLtW-juruCQ86rFU-uflIdGoi+gdb-at-[87.178.234.229]) by fwd14.t-online.de 5, 22 -- with esmtp id 1QX6of-1PiKEy0; Thu, 16 Jun 2011 09:16:37 +0200 5, 22 -- Message-ID: {4DF9ADD5.7020406-at-t-online.de} 5, 22 -- Date: Thu, 16 Jun 2011 09:16:37 +0200 5, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 5, 22 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microscopy-at-microscopy.com 5, 22 -- Subject: Re: [Microscopy] Sorvall MT-5000 microtome 5, 22 -- References: {201106152352.p5FNqqMq018283-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201106152352.p5FNqqMq018283-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-ID: VmlGNiZv8hB76Xez5EbVQ+OY0Tm-Jj6-+dvcGXLtW-juruCQ86rFU-uflIdGoi+gdb 5, 22 -- X-TOI-MSGID: 0ace31d8-3365-4255-bf8d-504b16b483da ==============================End of - Headers==============================
We have a Jeol 1200 EXII TEM and we were wondering if it is good enough in which to use a LaB6. The engineers seem to have mix opinions, as does the great google. I would like comments from anyone who has or has had a 1200 fitted with a LaB6. If you have, which ones? The microscope is behaving itself very well, no leaks everything is as good as it gets given its age. With one fitted do you notice any significant difference when working at the top end?
Thanks in advance.
John
==============================Original Headers============================== 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to 4, 30 -- :content-type; 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 30 -- d=gmail.com; s=gamma; 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= 4, 30 -- MIME-Version: 1.0 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} 4, 30 -- To: Microscopy-at-microscopy.com 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
LaB6 filament have to work in a good vacuum. There is some instruments like your TEM working with LaB6 in a gun pumped by DP or turbo, but most of the LaB6 TEM work with high vacuum in the gun provided by SIP. If your vacuum system is very good you may use LaB6 but you must understand that you will work on an edge. Anyway, you may try without danger for your instrument and you will see if LaB6 stay save during one year (good) or one month. Please note that you may use another wehnelt cap for this kind of filament and you have to change distance beetween filament tip and wehnelt cap. First eating of this filament must be done very slowy (about one hour) after almost one night of pumping. Working with LaB6 is very comfortable because the brigtness is very high and it may increase significantly top end result. Regards
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
--
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GAbEpl020489 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5GAb9nv007050; 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5GAb7iQ000928 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
} LaB6 filament have to work in a good vacuum. There is some instruments } like your TEM working with LaB6 in a gun pumped by DP or turbo, but most } of the LaB6 TEM work with high vacuum in the gun provided by SIP.
I would not recommend to work with a LaB6 in a (T)EM pumped by a DP or turbo only. For me, and from our 20yr experience, an IGP or ion getter pump (is SIP the same?) plus a nitrogen-trap is a must. Other people may comment on this or have other experience, though. The lifetime of a LaB6 in our TEM (heavily used by bio-people; though, no cryo) , pumped by ODP, plus IGP plus nitrogen trap (all are always on, during day time) is 3 years, up to 3.5 years, in the last 20 years of use, now. I was told that the lifetime in a turbo-pumped TEM (bio, too) is about .5 to 1 year only, at best. It is not my own experience. Everybody can decide what this means, and what to do.
} if your vacuum system is very good you may use LaB6 but you must understand } that you will work on an edge. TRUE } Anyway, you may try without danger for } your instrument and you will see if LaB6 stay save during one year } (good) or one month. see above ...
} First Heating of this filament must be done } very slowy (about one hour) true - but I suggest "slowly" is sufficient; we heat for about 15 min, after a LaB6 change.
} ... after almost one night of pumping. that depends - very much - on the quality of the pumping system and the status of the pumps and so on. And, how long the gun area was opened, for changing the filament. - Last week, in our machine, we reached the vacuum which "allows" us to start the LaB6 (with all pumps, ODP, IGP, nitrogen trap ON) after 30 to 60 minutes, only.
Kind regards, Reinhard Rachel
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 9, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Thu Jun 16 09:56:32 2011 9, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GEuVOk008667 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 09:56:31 -0500 9, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 9, 25 -- by localhost (Postfix) with SMTP id 5E2CE3876 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 16:56:26 +0200 (CEST) 9, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 9, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 42B213775 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 16:56:25 +0200 (CEST) 9, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 9, 25 -- with Novell_GroupWise; Thu, 16 Jun 2011 16:56:26 +0200 9, 25 -- Message-Id: {4DFA35C50200005400026970-at-gwsmtp1.uni-regensburg.de} 9, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 9, 25 -- Date: Thu, 16 Jun 2011 16:56:37 +0200 9, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 9, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 9, 25 -- Subject: LaB6 in a Jeol 1200ex2 9, 25 -- References: {201106161037.p5GAbVA1020810-at-ns.microscopy.com} 9, 25 -- In-Reply-To: {201106161037.p5GAbVA1020810-at-ns.microscopy.com} 9, 25 -- Mime-Version: 1.0 9, 25 -- Content-Type: text/plain; charset=US-ASCII 9, 25 -- Content-Disposition: inline 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5GEuVOk008667 ==============================End of - Headers==============================
We have the same model Jeol 1200 EXII as you do and it has worked flawlessly for 25 years with LaB6 filaments. (It has diffusion pumps not turbo). We get anywhere from 900 to 1500 hrs of livetime filament life - and that is running at fairly high emission currents of ~ 20µA. One caveat: it helps to keep a clean column (vacuum). We have a custom-built LN2 cold trap that lasts for up to 48 hrs. Hope this helps. Feel free to call me off-line if you need further discussion.
Peter
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Peter Ingram Sr. Physicist Adj. Professor of Pathology Duke University Medical Center PO Box 90319 DURHAM NC 27708-0319
The Cell: An Image Library is honored to be named a finalist in the website Labby Awards. Please help us win this award and vote for us at the site below. Please be patient if the site does not load right away and apologies for cross posting. Please tell your friends to vote for us as well.
I just wanted to report that we were able to resolve the image banding problem on our Gatan 794 controller.
It turns out that there is a 6800uF (30V) capacitor inside the camera controller that was adding about 2V! of noise to our system. This noise prevented the acquisition of a Gain Reference Image.
Many thanks to all Gatan folks that helped and especially to the Univ of Michigan who made a much needed controller available to us.
It turns out that this older vintage controller is out of "official production" and Gatan can help with repairs on a "best effort" basis, but you can not just go buy a new one if yours goes down. We also learned that the controller needs to be matched with your particular camera as it depends on which generation chip is used inside the controller.
Like so many things, it is was easy to find a solution one you know what you are looking for. That was certainly our case. When the camera is down, the TEM is down, hence our pointed interest.
A good electrics person with a hot soldering iron and the right capacitor can do the job in an hour. We took a little longer... please contact me if i can be of help
best regards
bryan tracy Spansion
==============================Original Headers============================== 10, 25 -- From Bryan.Tracy-at-spansion.com Thu Jun 16 15:02:01 2011 10, 25 -- Received: from USSVLMGTY02.spansion.com (ussvlmgty02.spansion.com [12.151.36.113]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GK20e2013196 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 15:02:01 -0500 10, 25 -- Received: from ussvlvexcasht2.spansion.com ([10.247.35.124]) 10, 25 -- by USSVLMGTY02.spansion.com with ESMTP; 16 Jun 2011 13:02:00 -0700 10, 25 -- Received: from US2K7EXMBPF1.spansion.com ([10.247.27.5]) by 10, 25 -- USSVLVEXCASHT2.spansion.com ([10.247.35.124]) with mapi; Thu, 16 Jun 2011 10, 25 -- 13:02:00 -0700 10, 25 -- From: "Tracy, Bryan" {Bryan.Tracy-at-spansion.com} 10, 25 -- To: "Listserver (Microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com} 10, 25 -- Date: Thu, 16 Jun 2011 13:01:58 -0700 10, 25 -- Subject: Image banding problem resolved on Gatan 794 controller 10, 25 -- Thread-Topic: Image banding problem resolved on Gatan 794 controller 10, 25 -- Thread-Index: AcwsYD9zeypVJRtRS72uuVqjP5KW9w== 10, 25 -- Message-ID: {F946A2B523FFC24EABFA434A3D143C820DF725E780-at-US2K7EXMBPF1.spansion.com} 10, 25 -- Accept-Language: en-US 10, 25 -- Content-Language: en-US 10, 25 -- X-MS-Has-Attach: 10, 25 -- X-MS-TNEF-Correlator: 10, 25 -- acceptlanguage: en-US 10, 25 -- Content-Type: text/plain; charset="us-ascii" 10, 25 -- MIME-Version: 1.0 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5GK20e2013196 ==============================End of - Headers==============================
Dear Chuan Zhang, I am nowhere near being an expert on this, but, since no one else has replied, I'll take a shot. Have you tried making the exposure time smaller, or, equivalently, making the beam dimmer? Since the CCD is more sensitive than the phosphor, you will need to have fewer electrons/pixel to get similar visibility. Increasing the camera length would be another way to accomplish this. Yours, Bill
X-from: chuanz-at-uci.edu To: William.F.Tivol-at-aero.org
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Greetings listers,
I am trying to acquire HOLZ lines in 000 transmitted spot of CBED patterns for point group determination purpose, but have been experiencing difficulties in acquiring/recording images of HOLZ lines. The TEM I am using is Philips EM-20 with Gatan camera. On the phosphor screen, I was able to see HOLZ lines for higher index zone axis patterns and they seems to be clearer under 80kV than 200kV. However, when I inserted the Gatan camera to take pictures, I noticed that the quality of the diffraction images was too bad - the HOLZ lines were blurry, and some areas were too bright, making the nearby HOLZ lines invisible. Does anyone on this list have similar problem before? Any suggestions on how to record sharp/clear HOLZ images would be much appreciated!
Best regards,
Chuan Zhang Graduate Student Researcher, Dept. of Chemical Engineering and Materials Science, University of California, Irvine
==============================Original Headers============================== 4, 33 -- From zhangchuan827-at-gmail.com Thu Jun 16 01:52:57 2011 4, 33 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 4, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G6qurU001454 4, 33 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:52:57 -0500 4, 33 -- Received: by vxk20 with SMTP id 20so1186653vxk.0 4, 33 -- for {Microscopy-at-microscopy.com} ; Wed, 15 Jun 2011 23:52:56 -0700 (PDT) 4, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 33 -- d=gmail.com; s=gamma; 4, 33 -- h=domainkey-signature:mime-version:sender:from:date 4, 33 -- :x-google-sender-auth:message-id:subject:to:content-type; 4, 33 -- bh=3BCUubw8UQvFaIuaHjXqwmLSxm5++INmIemWH5pYPSU=; 4, 33 -- b=YGaDpTcRnzV1eeI9BbQ3b6SJTW+gF13/ox5hrM4Fz9xttahrmh2qUVAbLnS2w/+Hhe 4, 33 -- 3wcfAEJnSwatogkn8/UYx85qkmJ5FJ942Y9zfiPpOkfnQHNMOCWn652tNuuFxedj70yh 4, 33 -- beEiCB5Qq49d1o8vXW9/HUD+DAtqN3mtBpzTI= 4, 33 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 4, 33 -- d=gmail.com; s=gamma; 4, 33 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 4, 33 -- :subject:to:content-type; 4, 33 -- b=Kf16Bnn/c9C9N+fqLfrgd3f1qYT7bJcKnnVZpIXk5KkByvAK6uJw/OcecVMYeSmCIz 4, 33 -- e1anKyVhbci0T86boI5wWmnMJJN8m1QX548MKW9t57MxdH/m+M/R+L60GyLugmCFHRcJ 4, 33 -- 4u+sGiSXMtry6kHcVCXMq7apyx9V5fygICwHU= 4, 33 -- Received: by 10.52.187.164 with SMTP id ft4mr734594vdc.59.1308207176102; Wed, 4, 33 -- 15 Jun 2011 23:52:56 -0700 (PDT) 4, 33 -- MIME-Version: 1.0 4, 33 -- Sender: zhangchuan827-at-gmail.com 4, 33 -- Received: by 10.52.113.35 with HTTP; Wed, 15 Jun 2011 23:52:16 -0700 (PDT) 4, 33 -- From: Chuan Zhang {chuanz-at-uci.edu} 4, 33 -- Date: Wed, 15 Jun 2011 23:52:16 -0700 4, 33 -- X-Google-Sender-Auth: f54XRRGmX0SHm0dNdP4cGNdXWRg 4, 33 -- Message-ID: {BANLkTinLay6CrYhPqCDEPqtSk6ir4z4OkQ-at-mail.gmail.com} 4, 33 -- Subject: TEM - Problem with Acquiring HOLZ lines in 000 spot 4, 33 -- To: Microscopy-at-microscopy.com 4, 33 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5H5jYa5012073 for {MicroscopyListserverArchive2-at-microscopy.com} ; Fri, 17 Jun 2011 00:45:34 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p5H5jYCT012071; Fri, 17 Jun 2011 00:45:34 -0500
Use of LaB6 cathode in a vacuum system without an IGP and differential pumping is possible but impractical. Even if all following rules will be enforced:
- have LN trap cold before heating filament; - turn off filament heating before specimen exchange; - never heat up filament before gun/column vacuum fully recovers after specimen (or film) exchange,
LaB6 will not likely last for even 1000 hours. Much less at higher emission setting. Vacuum for LaB6 must be better then 10-6 Torr. Very difficult to maintain without IGP and differential pumping system. I consider LaB6 use justified when it lasts at least 2000 hours at low/medium emission. My average service time experience for LaB6 is above 3500 hours in a UHV system, with the record approx. 7000 hours and still going. This particular Denka cathode was installed on Philips CM-12 in 1997 and still works 14 years later.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 6/16/2011 4:28 AM, john.mitchels-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 26 -- From vitalylazar-at-att.net Fri Jun 17 00:45:34 2011 5, 26 -- Received: from fmailhost01.isp.att.net (fmailhost01.isp.att.net [204.127.217.101]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5H5jX3J012068 5, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 00:45:33 -0500 5, 26 -- DKIM-Signature: v=1; q=dns/txt; d=att.net; s=dkim01; 5, 26 -- i=vitalylazar-at-att.net; a=rsa-sha256; c=relaxed/relaxed; 5, 26 -- t=1308289534; h=Content-Transfer-Encoding:Content-Type: 5, 26 -- In-Reply-To:References:Subject:To:MIME-Version:From:Date: 5, 26 -- Message-ID; bh=CcBb0s9Bj+M4z7dhk0Mtg/iqokLSOfXCu0sxba75hPw=; b=JWr 5, 26 -- 60WkDWSUKE4e+8ELhpWSo35LFT+AyrNXHY17FfhiWVNXItpCyi2jOAcyJa8rK16RJA6 5, 26 -- oqmPuOeLqvHKS67A== 5, 26 -- Received: from [192.168.0.101] (adsl-74-190-38-67.asm.bellsouth.net[74.190.38.67]) 5, 26 -- by worldnet.att.net (frfwmhc01) with ESMTP 5, 26 -- id {20110617054532H0100ks05ae} ; Fri, 17 Jun 2011 05:45:33 +0000 5, 26 -- X-Originating-IP: [74.190.38.67] 5, 26 -- Message-ID: {4DFAE9F9.8090000-at-att.net} 5, 26 -- Date: Fri, 17 Jun 2011 01:45:29 -0400 5, 26 -- From: Vitaly Feingold {vitalylazar-at-att.net} 5, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) Gecko/20110414 Thunderbird/3.1.10 5, 26 -- MIME-Version: 1.0 5, 26 -- To: john.mitchels-at-gmail.com, Microscopy Listserver {Microscopy-at-microscopy.com} 5, 26 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 5, 26 -- References: {201106160828.p5G8SUpQ004168-at-ns.microscopy.com} 5, 26 -- In-Reply-To: {201106160828.p5G8SUpQ004168-at-ns.microscopy.com} 5, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There are four points to consider when taking convergent beam electron diffraction patterns (CBED) on a digital camera:
1. Correct focus (diffraction lens). If your camera is far from the viewing screen, then the CBED pattern can appear to be out of focus and the HOLZ deficiency lines are quickly lost. I have used a Gatan Imaging Filter to acquire (energy filtered) CBED patterns and always have to fine-tune the diffraction focus to get a nice sharp (000) CBED disc- the condenser aperture should appear sharp when the diffraction focus is correct.
2. Sampling rate. If your binning is too high or the camera length is too small the modulation transfer function (MTF) of the camera may be blurring the HOLZ deficiency lines too much. Try using single binning and/or a higher camera length. You may have to try several different areas because HOLZ deficiency lines tend to narrow with thicker areas.
3. Dynamic range. Because of the highly inhomogeneous distribution of intensity in a diffraction pattern, it is easy to saturate the camera. I have found using multiple exposures with a small acquisition time to help tremendously. For example, I have taken diffraction patterns with } 100,000 counts per pixel on a 16-bit camera (max. 16396) by using the multiple frames option (DM} Camera} Acquisition Parameters} Acquire} Processing} Frame Sum (No. of Frames). Ten or twenty frames can make a significant difference to the visibility of weak features.
4. Spot size (used in conjunction with point 3). I've found that using a smaller probe with less current leads to better CBED patterns (and using a larger exposure time). Most samples have a thickness gradient so the CBED pattern you see are an incoherent mixture of CBED patterns from each point on the sample that is irradiated, i.e. smaller probes 'average out' the CBED pattern over a smaller area. However, if your samples are flat (and clean) this shouldn't make too much difference.
I hope these help.
Yours, Jon Barnard
} Greetings listers, } } I am trying to acquire HOLZ lines in 000 transmitted spot of } CBED patterns for point group determination purpose, but have been } experiencing difficulties in acquiring/recording images of HOLZ lines. } The TEM I am using is Philips EM-20 with Gatan camera. On the phosphor } screen, I was able to see HOLZ lines for higher index zone axis } patterns and they seems to be clearer under 80kV than 200kV. However, } when I inserted the Gatan camera to take pictures, I noticed that the } quality of the diffraction images was too bad - the HOLZ lines were } blurry, and some areas were too bright, making the nearby HOLZ lines } invisible. Does anyone on this list have similar problem before? Any } suggestions on how to record sharp/clear HOLZ images would be much } appreciated! } } Best regards, } } Chuan Zhang } Graduate Student Researcher, } Dept. of Chemical Engineering and Materials Science, } University of California, Irvine
==============================Original Headers============================== 10, 28 -- From jsb43-at-hermes.cam.ac.uk Fri Jun 17 03:44:54 2011 10, 28 -- Received: from ppsw-41.csi.cam.ac.uk (ppsw-41.csi.cam.ac.uk [131.111.8.141]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5H8irSQ029963 10, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 03:44:54 -0500 10, 28 -- X-Cam-AntiVirus: no malware found 10, 28 -- X-Cam-SpamDetails: not scanned 10, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 10, 28 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:46568) 10, 28 -- by ppsw-41.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25) 10, 28 -- with esmtpa (EXTERNAL:jsb43) id 1QXUfc-0008AN-Sv (Exim 4.72) 10, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 17 Jun 2011 09:44:52 +0100 10, 28 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk) 10, 28 -- with local (PRAYER:jsb43) id 1QXUfc-0000dX-Uf (Exim 4.67) 10, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 17 Jun 2011 09:44:52 +0100 10, 28 -- Received: from [128.232.229.182] by webmail.hermes.cam.ac.uk 10, 28 -- with HTTP (Prayer-1.3.3); 17 Jun 2011 09:44:51 +0100 10, 28 -- Date: 17 Jun 2011 09:44:51 +0100 10, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 10, 28 -- To: chuanz-at-uci.edu 10, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 10, 28 -- Subject: Re: TEM - Problem with Acquiring HOLZ lines in 000 spot 10, 28 -- Message-ID: {Prayer.1.3.3.1106170944510.19799-at-hermes-2.csi.cam.ac.uk} 10, 28 -- In-Reply-To: {201106160659.p5G6xoLU010985-at-ns.microscopy.com} 10, 28 -- References: {201106160659.p5G6xoLU010985-at-ns.microscopy.com} 10, 28 -- X-Mailer: Prayer v1.3.3 10, 28 -- Mime-Version: 1.0 10, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 10, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
Many thanks to all which sent me some documentation and advices. I've now the manual and some pictures of the head, which seems to be complete and in working conditions.
Best regards to all !
J. Faerber IPCMS-GSI (Institut de Physique et Chimie des Matériaux de Strasbourg Groupe Surface et Interfaces) 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 15/06/2011 13:57, jacques.faerber-at-ipcms.u-strasbg.fr a écrit : } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all } } Recently I got a Edwards S150 carbon coating unit (Part Nr E087-23-003), } the unit alone without the vaccum vessel, i.e. the power supply with two } cables fixed to an Al flange, with some sort of holder for the carbon } rods on it. } As it look like, and compared to a picture found on internet (on an } auction web site...), I fear that my head is not complete. } } So I'm looking for a picture, or a sketch, of a garanted working head, } and if possible for the a copy of the manual (paper or pdf file). } } Does someone on the list have this stuff ? } } Many thanks in advance }
==============================Original Headers============================== 6, 29 -- From jacques.faerber-at-ipcms.u-strasbg.fr Fri Jun 17 03:46:32 2011 6, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5H8kWHx000477 6, 29 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 03:46:32 -0500 6, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 6, 29 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id p5H8kUWi032233 6, 29 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 10:46:31 +0200 (CEST) (envelope-from jacques.faerber-at-ipcms.u-strasbg.fr) 6, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 6, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 6, 29 -- (No client certificate requested) 6, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id 6B0DB32001 6, 29 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 10:41:58 +0200 (CEST) 6, 29 -- Message-ID: {4DFB143A.5080008-at-ipcms.u-strasbg.fr} 6, 29 -- Date: Fri, 17 Jun 2011 10:45:46 +0200 6, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 6, 29 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.17) Gecko/20110424 Thunderbird/3.1.10 6, 29 -- MIME-Version: 1.0 6, 29 -- To: microscopy-at-microscopy.com 6, 29 -- Subject: Thanks : Edwards S150 carbon coating unit 6, 29 -- References: {201106151157.p5FBv0Be001716-at-ns.microscopy.com} 6, 29 -- In-Reply-To: {201106151157.p5FBv0Be001716-at-ns.microscopy.com} 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-IPCMS-MailScanner: Found to be clean 6, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.u-strasbg.fr 6, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Fri, 17 Jun 2011 10:46:31 +0200 (CEST) 6, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=T_RP_MATCHES_RCVD, 6, 29 -- USER_IN_WHITELIST autolearn=disabled version=3.3.1 6, 29 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
I find that I have the entire MT-5000 service manual. It is about 200 pages. I'm not sure I am offering to scan in and PDF the whole thing, unless I find someone who is bored (I am not). Do you have specific questions I can look up for you now? If not, I can work on scanning the thing in over a period of time and then send it to you.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 24 -- From tina-at-pbrc.hawaii.edu Fri Jun 17 16:54:21 2011 4, 24 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5HLsKP8025440 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 16:54:20 -0500 4, 24 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 24 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p5HLsHYJ007678 4, 24 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 24 -- Fri, 17 Jun 2011 11:54:17 -1000 (HST) 4, 24 -- Received: from localhost (tina-at-localhost) 4, 24 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p5HLsGnR007674; 4, 24 -- Fri, 17 Jun 2011 11:54:17 -1000 (HST) 4, 24 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 24 -- Date: Fri, 17 Jun 2011 11:54:16 -1000 (HST) 4, 24 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 24 -- X-X-Sender: tina-at-b1000 4, 24 -- To: tbogea-at-interchange.ubc.ca 4, 24 -- cc: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 24 -- Subject: Re: [Microscopy] Sorvall MT-5000 microtome 4, 24 -- In-Reply-To: {Pine.GSO.4.64.1106151352510.10616-at-b1000} 4, 24 -- Message-ID: {Pine.GSO.4.64.1106171151150.5925-at-b1000} 4, 24 -- References: {201106152349.p5FNnUeW013437-at-ns.microscopy.com} 4, 24 -- {Pine.GSO.4.64.1106151352510.10616-at-b1000} 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Hello, I have a Kevex model 2003 preamplifier that I'm trying to re-purpose into a completely different project I'm working on. Does anybody have a pinout for the external and internal connections, i.e. what pins get what voltages on the five pin power connector, and what parts of the FET do the internal wires go to?
Thanks,
Justin A. Kraft
==============================Original Headers============================== 3, 37 -- From kraftpiano-at-gmail.com Fri Jun 17 17:45:31 2011 3, 37 -- Received: from mail-yi0-f41.google.com (mail-yi0-f41.google.com [209.85.218.41]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5HMjVpu009467 3, 37 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 17:45:31 -0500 3, 37 -- Received: by yia13 with SMTP id 13so2073369yia.0 3, 37 -- for {microscopy-at-microscopy.com} ; Fri, 17 Jun 2011 15:45:31 -0700 (PDT) 3, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 37 -- d=gmail.com; s=gamma; 3, 37 -- h=domainkey-signature:from:content-type:content-transfer-encoding 3, 37 -- :subject:date:message-id:to:mime-version:x-mailer; 3, 37 -- bh=8mCCOUc4p7eB/tCLgXsphNdDDtc1Nig8S7RRy6eg8J4=; 3, 37 -- b=G2I12xvPQW/MdrpIFCvETLig4RtF+BafJ2CAkeqOX8JJb9po9uzpOiske3lWgb/GK7 3, 37 -- dNUwlj4xjyz1OLo3TvTzwwgf9D5QU64+Qxu5HnCu6XqTEMZm6j8HMAoNER7jkqxuvcC5 3, 37 -- mXZJWeVe6ArsYPzAuUJ8ZlHdFNHWdLjTPGMcY= 3, 37 -- DomainKey-Signature: a=rsa-sha1; c=nofws; 3, 37 -- d=gmail.com; s=gamma; 3, 37 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 3, 37 -- :to:mime-version:x-mailer; 3, 37 -- b=TBBLZcwt9I6ODgM/u3dcWqtnFWkd/1ol7IvzaCiwg+5alBONpsLM8mN5jfTjTc16dc 3, 37 -- k1VRc/EWpBYD2SBN1gTF+YLqC24tpHqv9GV4R1XwhCmEEgF7szASzecWRUXwPGwHAcpk 3, 37 -- 5zjnKCk3MBYL+DXqxtcFS/pj7wA8veBseyriY= 3, 37 -- Received: by 10.236.182.130 with SMTP id o2mr4492333yhm.77.1308350727873; 3, 37 -- Fri, 17 Jun 2011 15:45:27 -0700 (PDT) 3, 37 -- Received: from [10.101.1.113] (c-66-176-126-164.hsd1.fl.comcast.net [66.176.126.164]) 3, 37 -- by mx.google.com with ESMTPS id k10sm2044571yhj.64.2011.06.17.15.45.26 3, 37 -- (version=TLSv1/SSLv3 cipher=OTHER); 3, 37 -- Fri, 17 Jun 2011 15:45:26 -0700 (PDT) 3, 37 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 37 -- Content-Type: text/plain; charset=us-ascii 3, 37 -- Subject: EDS system (Kevex) pinout information. 3, 37 -- Date: Fri, 17 Jun 2011 18:45:24 -0400 3, 37 -- Message-Id: {A705ACEE-748A-4FA8-8982-6DE525A32178-at-gmail.com} 3, 37 -- To: microscopy-at-microscopy.com 3, 37 -- Mime-Version: 1.0 (Apple Message framework v1084) 3, 37 -- X-Mailer: Apple Mail (2.1084) 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5HMjVpu009467 ==============================End of - Headers==============================
We have a Rontec (now Bruker) Xflash Detector 1000 Series attached to our Cameca SX-100 electron probe that is no longer detecting X-ray signals following a series of schedule power outages over the last couple weeks. The system was completely shut down during each power outage to prevent any electrical damage.
The Bruker software (Esprit 1.8) appears to communicate with the detector and we are able to read noise (~ 7 cps/eV) at the low ( { 0.5 keV) energy range, but higher energy x-rays are not being detected when we analyze a sample (I've been looking at benitoite for the tests to make sure that we have a beam).
I'm not aware of anything like a closed aperture or structure blocking x-rays from reaching the detector window. The cooling system also appears to be working and the detected temperature is -15 *C.
I have turned the EDS system off overnight and reset it this morning, I've also reset the electronics to the entire probe but nothing seems to be working.
Has anyone else encountered this type of problem?
Any help would/insight would be appreciated!
-Dan
-- Dan Ruscitto, Ph.D.
Laboratory Manager 1W13 Jonsson-Rowland Science Center Earth& Environmental Sciences Rensselaer Polytechnic Institute 110 8th St. Troy NY 12180 Ph: 518-276-2372 Fax: 518-276-2012
==============================Original Headers============================== 11, 23 -- From ruscid2-at-rpi.edu Mon Jun 20 08:32:18 2011 11, 23 -- Received: from smtp8.server.rpi.edu (smtp8.server.rpi.edu [128.113.2.228]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5KDWHgM029807 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Jun 2011 08:32:18 -0500 11, 23 -- Received: from [128.113.7.86] (rotameter-25.dynamic.rpi.edu [128.113.7.86]) 11, 23 -- (authenticated bits=0) 11, 23 -- by smtp8.server.rpi.edu (8.13.1/8.13.1) with ESMTP id p5KDWDbM011938 11, 23 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 11, 23 -- for {microscopy-at-microscopy.com} ; Mon, 20 Jun 2011 09:32:14 -0400 11, 23 -- Message-ID: {4DFF4BDD.6050503-at-rpi.edu} 11, 23 -- Date: Mon, 20 Jun 2011 09:32:13 -0400 11, 23 -- From: Dan Ruscitto {ruscid2-at-rpi.edu} 11, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.17) Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 11, 23 -- MIME-Version: 1.0 11, 23 -- To: microscopy-at-microscopy.com 11, 23 -- Subject: Bruker EDS Troubles 11, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 23 -- Content-Transfer-Encoding: 7bit 11, 23 -- X-Bayes-Prob: 0.0001 (Score 0) 11, 23 -- X-RPI-SA-Score: 1.40 (*) [Hold at 12.00] RATWARE_GECKO_BUILD,22490(-25) 11, 23 -- X-CanItPRO-Stream: outgoing 11, 23 -- X-Canit-Stats-ID: Bayes signature not available 11, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.113.2.228 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nbrowning-at-ucdavis.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Abstracts and Registration for FEMMS 2011
Message: TOP OF THE NEWS
-- FEMMS REGISTRATION IS NOW OPEN -- CALL FOR ABSTRACTS -- FEMMS2011 INVITED SPEAKERS
FEMMS INFO THAT YOU NEED TO KNOW
-- BOOK YOUR ROOM ONLINE -- TRAVEL TO FEMMS2011
-- FEMMS REGISTRATION
Online registration for FEMMS2011 is now available http://www.regonline.com/femms2011
-- CALL FOR ABSTRACTS
Abstracts for the 13th Frontiers of Electron Microscopy in Materials Science - FEMMS2011 are due no later than July 31, 2011. Because participation is limited, we encourage early submission. Information can be found at http://femms2011.llnl.gov/abstracts. The abstract template is available at http://femms2011.llnl.gov/abstracts/Abstract_template.doc. Abstracts are to be submitted by email to mailto:femms2011-at-llnl.gov with a subject of ABSTRACT FOR FEMMS2011.
-- FEMMS2011 INVITED SPEAKERS
The organizers of the 11th Frontiers of Electron Microscopy in Materials Science - FEMMS2011 have assembled a remarkable list of invited speakers for this meeting. Check them out at http://femms2011.llnl.gov/program. Read their bios at http://femms2011.llnl.gov/program/invited.shtml
-- BOOK YOUR ROOM ONLINE
To take advantage of the special conference rate at the DoubleTree Hotel Sonoma Wine Country, book your room online at http://tinyurl.com/bookroomforFEMMS (link goes to Hilton Hotels)
-- TRAVEL TO THE CONFERENCE
It's convenient to get to the DoubleTree Hotel Sonoma Wine Country from any of the Bay Area airports. Get your travel hints here http://femms2011.llnl.gov/travelinfo/
13th Frontiers of Electron Microscopy in Materials Science - FEMMS2011 September 18-23, 2011 DoubleTree Hotel Sonoma County One Doubletree Drive Rohnert Park, CA 94928 http://femms2011.llnl.gov
Major support for FEMMS2011 is provided by Lawrence Livermore National Laboratory (LLNL), Oak Ridge National Laboratory (ORNL), Pacific Northwest National Laboratory (PNNL), and the National Nuclear Security Administration (NNSA)
I have 2 not completely related questions but please allow me to pack them up in one single email in order to avoid an overflow of junk out-of-office-you-know-what-I-mean replies.
1. I would be interested in quantifying cells in 96 MTP wells directly, which means avoiding the so-called "viability" or "proliferation" ELISA assay since the treatment to the cells probably influences their metabolism (a consideration which rarely bothers authors of papers related to oxydative stres f.ex.).
One way would be a software-driven automatic cell counting associated with fluorescence microscopy. We have a Zeiss axiovert 200M so I think we could try a good software. Now the question is: which software is good? Optimally it should be free and do the job but we definitely will give preference to "doing the job" than being free. I have read a paper about CellProfiler. I would be interested in any feedback in this domain. The cells to be counted are very different eucaryotic cells: HepG2, Caco-2, MCF-10A and other cell lines as well as primary fibroblasts and keratinocytes.
2. I am searching a source for a Brdu Hybridoma. The idea is to develop myself an ELISA method to quantify the cell proliferation in culture because I would like to use it routinely. Each company sell their own kit but actually the key point of these kits is the specific primary antibody. If I can get hold of a hybridoma producing a primary which can do the job, bingo! The hybridoma must be available for purchase by a for-profit organisation, though. It is important to state it because sometimes a product is restricted to academic research (like HaCat cell f.ex.).
Many thanks in advance
Stephane
==============================Original Headers============================== 9, 34 -- From nizets2-at-yahoo.com Tue Jun 21 04:03:11 2011 9, 34 -- Received: from nm9.bullet.mail.sp2.yahoo.com (nm9.bullet.mail.sp2.yahoo.com [98.139.91.79]) 9, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p5L93BsY015231 9, 34 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jun 2011 04:03:11 -0500 9, 34 -- Received: from [98.139.91.65] by nm9.bullet.mail.sp2.yahoo.com with NNFMP; 21 Jun 2011 09:03:11 -0000 9, 34 -- Received: from [98.139.91.42] by tm5.bullet.mail.sp2.yahoo.com with NNFMP; 21 Jun 2011 09:03:11 -0000 9, 34 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 21 Jun 2011 09:03:11 -0000 9, 34 -- X-Yahoo-Newman-Property: ymail-3 9, 34 -- X-Yahoo-Newman-Id: 17737.20274.bm-at-omp1042.mail.sp2.yahoo.com 9, 34 -- Received: (qmail 55456 invoked by uid 60001); 21 Jun 2011 09:03:10 -0000 9, 34 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1308646990; bh=1hY9uvS9uHBS4gyaL7C2EciNHQEKKkxeQ4qvZ5zDAns=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=2y7DoXvvQONfJSqPuIjQaICLF4xw1psCLity89qVG0HR449QtmMmYXUYy4qbnI3XVBIrqrU/aanIhpu4NpuKLTt/c3KenVRP6WpFQQ22b8kO3yALVWo0KZW1PSM2AYLNAjMYJP9HhZo4BpSuSCMskL1ALoJruCS6Ak0NII8SiNc= 9, 34 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 9, 34 -- s=s1024; d=yahoo.com; 9, 34 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 9, 34 -- b=lKc1rlPp3hu8h2qc8UDm9l5bvWs2q/+3E9s2uMacHLDbblDe+cUPBjAed+dJSEWt4pnXvPG8HMEO/XWNU/Kqfb9p4WuEQobbCqV91yGhsbsLbX2DU36cns5Am+Njaa/U9bbLhhLGc5uqdOPN/uPKyz0HBBM06TTn4+MOGe2dQo8=; 9, 34 -- X-YMail-OSG: dSyY7uIVM1mzKHNn.nyF3W1aELsnnWLjKQiCYJU64YVehbf 9, 34 -- 51IKA4y14RZXgzBEY0jQS8A698UAZ.NP_o0lydnwJmHwcO83cZJ_1ZkxE_cJ 9, 34 -- 1xE47dmeCtfNgEI.ajRjt_rWCEoH0SAaKAWZvzyqIToNBzGg5wLjdb3KmOzN 9, 34 -- 1R_UgatFYsjtiuCdhCt8MWMbS43aN9Sl9J77qBMCXmn1MAt2zmdJdTw49GQA 9, 34 -- _J27P0timZok07pGjZtWbtzu2jTTt1GLTcL_JVk7T.GmNrwJTppww6_WWEWK 9, 34 -- tlVLs.mp1etWPM2WGf3xSikQIfHO9ruxebVC4hxqBd15OE57rp_XRJtWO7Zf 9, 34 -- 4hSobHIFjWRiru82LukgRZXC84M_SfAPs6S2lyNcSlNuhjHPnbZWLsb8KkmE 9, 34 -- KHcUmaD9yeUzzUw-- 9, 34 -- Received: from [80.122.101.100] by web110801.mail.gq1.yahoo.com via HTTP; Tue, 21 Jun 2011 02:03:10 PDT 9, 34 -- X-Mailer: YahooMailRC/570 YahooMailWebService/0.8.112.307740 9, 34 -- Message-ID: {1308646990.53754.YahooMailRC-at-web110801.mail.gq1.yahoo.com} 9, 34 -- Date: Tue, 21 Jun 2011 02:03:10 -0700 (PDT) 9, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com} 9, 34 -- Subject: 2 questions. automatic cell counting and BrdU hybridoma 9, 34 -- To: microscopy-at-microscopy.com 9, 34 -- MIME-Version: 1.0 9, 34 -- Content-Type: text/plain; charset=iso-8859-1 9, 34 -- Content-Transfer-Encoding: 8bit 9, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5L93BsY015231 ==============================End of - Headers==============================
Dear All, I am in the progress of developing a modular remote interface for the Philips (FEI) SEM 5x5 (types 515, 525, 545).
The remote control will handle: - zoom function on the magnification range (1x to 8x in 255 steps) - beamshift x and y in 255 steps - read and set final lens current in 65536 steps, if a FCA unit is existing in the scope - gain and blacklevel on SE detector in 1024 steps - gain and blacklevel on BSE-detector in 1024 steps - remote control of the 6" motorstage via RS 232 - remote control of customized piezo stages
With an existing CIF unit (Computer interface for the 5x5, made by Philips), you will also be able to remote: - high voltage settings - condensor settings - set and read magnification
Anybody out there who is interested in joining the project or might contribute some thoughts?
Best wishes, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jgoolsby-at-paragonbioservices.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jgoolsby-at-paragonbioservices.com Name: James Goolsby
Organization: Paragon Bioservices
Title-Subject: [Filtered] EM Position in Baltimore MD
Message: An EM position is opening at a Contract Research and Manufacturing Organization (CRO/CMO) in Baltimore Maryland. Paragon Bioservices is a rapidly growing company that does pre-clinical R&D and GMP manufacturing. EM is among our array of analytics that is expanding and we are looking for a technician to help handle the increased capacity. Experience with biological TEM sample preparation is a must (1-2+ years). Additional diverse skills in biological R&D techniques would be welcome.
For more information, or to send a CV, email to: Jgoolsby-at-paragonbioservices.com
Hi, I wonder if you have encountered that problem and might have a solution. We have H7000 and inserting a specimen injector became problematic. After specimen chamber is evacuated I turn the specimen injector and I have a strong resistance as if something is holding the injector locked in place and does not let it go. I have to use force and that causes stage tilt to change. I don’t have this problem when I am taking the specimen out of the microscope. Do you have any suggestions and how it can be fixed? Thanks Dorota
Like Nicolas said here below, it's all a matter of vacuum. Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs usually have a filament life of way above 1000 hours. If you take the same SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get way above 1000 hours with the tungsten filament.
So, in theory if your SEM gun has a very good vacuum, you should be getting a very high lifetime from each tungsten filament. If not, you could expect to get good result with the LaB6 filemant, but keep the lifetime of your current tungsten one. Keep in mind that the LaB6 filament is exponentially more expensive than tungsten.
It'd be best to consult with Jeol in any case before you decide to do this.
Regards, Roy
-----Original Message----- X-from: Nicolas.Stephant-at-univ-nantes.fr [mailto:Nicolas.Stephant-at-univ-nantes.fr] Sent: Thursday, June 16, 2011 1:45 PM To: Roy Golombick
Hi John
LaB6 filament have to work in a good vacuum. There is some instruments like your TEM working with LaB6 in a gun pumped by DP or turbo, but most of the LaB6 TEM work with high vacuum in the gun provided by SIP. If your vacuum system is very good you may use LaB6 but you must understand that you will work on an edge. Anyway, you may try without danger for your instrument and you will see if LaB6 stay save during one year (good) or one month. Please note that you may use another wehnelt cap for this kind of filament and you have to change distance beetween filament tip and wehnelt cap. First eating of this filament must be done very slowy (about one hour) after almost one night of pumping. Working with LaB6 is very comfortable because the brigtness is very high and it may increase significantly top end result. Regards
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
--
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GAbEpl020489 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5GAb9nv007050; 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5GAb7iQ000928 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From Roy-at-picotech.co.il Wed Jun 22 09:29:42 2011 21, 25 -- Received: from mail.picotech.co.il (mail.picotech.co.il [212.143.255.226]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5METfrh003527 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 09:29:41 -0500 21, 25 -- Received: from SERVER01.picotech.local ([192.168.1.2]) by server01 21, 25 -- ([192.168.1.2]) with mapi; Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- From: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 25 -- Disposition-Notification-To: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- Date: Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Topic: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Index: AcwsEjA2FcR8LNxjSzquPhESHEjIbQE1a7nA 21, 25 -- Message-ID: {ABB156E109DC9A42B8109368CA6F4895178F899703-at-server01} 21, 25 -- References: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- In-Reply-To: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- Accept-Language: en-US 21, 25 -- Content-Language: en-US 21, 25 -- X-MS-Has-Attach: 21, 25 -- X-MS-TNEF-Correlator: 21, 25 -- acceptlanguage: en-US 21, 25 -- Content-Type: text/plain; charset="iso-8859-1" 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5METfrh003527 ==============================End of - Headers==============================
Roy, I beg to differ. I have not seen any significant increase in tungsten filament life in an ion-pumped gun versus a normal gun in good condition in an SEM. Poor vacuum will definitely shorten a W filament's life, but a better vacuum makes very little difference due to the fact that W filaments run hotter than LaB6 and evaporate faster. The reason poor vacuum accelerates their demise is due to oxidation on top of evaporation.
No W filament I've ever seen has lasted more than about 300 hours at operating temperature in an SEM, 10E-7T vacuum notwithstanding. My observations may not hold for a TEM because TEMs run at lower temperatures/emission currents and therefore evaporation rates should be significantly lower.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] Sent: Wednesday, June 22, 2011 10:31 AM To: kenconverse-at-qualityimages.biz
Hi John.
Like Nicolas said here below, it's all a matter of vacuum. Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs usually have a filament life of way above 1000 hours. If you take the same SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get way above 1000 hours with the tungsten filament.
So, in theory if your SEM gun has a very good vacuum, you should be getting a very high lifetime from each tungsten filament. If not, you could expect to get good result with the LaB6 filemant, but keep the lifetime of your current tungsten one. Keep in mind that the LaB6 filament is exponentially more expensive than tungsten.
It'd be best to consult with Jeol in any case before you decide to do this.
Regards, Roy
-----Original Message----- X-from: Nicolas.Stephant-at-univ-nantes.fr [mailto:Nicolas.Stephant-at-univ-nantes.fr] Sent: Thursday, June 16, 2011 1:45 PM To: Roy Golombick
Hi John
LaB6 filament have to work in a good vacuum. There is some instruments like your TEM working with LaB6 in a gun pumped by DP or turbo, but most of the LaB6 TEM work with high vacuum in the gun provided by SIP. If your vacuum system is very good you may use LaB6 but you must understand that you will work on an edge. Anyway, you may try without danger for your instrument and you will see if LaB6 stay save during one year (good) or one month. Please note that you may use another wehnelt cap for this kind of filament and you have to change distance beetween filament tip and wehnelt cap. First eating of this filament must be done very slowy (about one hour) after almost one night of pumping. Working with LaB6 is very comfortable because the brigtness is very high and it may increase significantly top end result. Regards
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
--
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GAbEpl020489 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5GAb9nv007050; 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5GAb7iQ000928 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Ste phant-at-univ-nantes.fr} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From Roy-at-picotech.co.il Wed Jun 22 09:29:42 2011 21, 25 -- Received: from mail.picotech.co.il (mail.picotech.co.il [212.143.255.226]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5METfrh003527 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 09:29:41 -0500 21, 25 -- Received: from SERVER01.picotech.local ([192.168.1.2]) by server01 21, 25 -- ([192.168.1.2]) with mapi; Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- From: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 25 -- Disposition-Notification-To: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- Date: Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Topic: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Index: AcwsEjA2FcR8LNxjSzquPhESHEjIbQE1a7nA 21, 25 -- Message-ID: {ABB156E109DC9A42B8109368CA6F4895178F899703-at-server01} 21, 25 -- References: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- In-Reply-To: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- Accept-Language: en-US 21, 25 -- Content-Language: en-US 21, 25 -- X-MS-Has-Attach: 21, 25 -- X-MS-TNEF-Correlator: 21, 25 -- acceptlanguage: en-US 21, 25 -- Content-Type: text/plain; charset="iso-8859-1" 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5METfrh003527 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 10:06:24 2011 33, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MF6NHc030811 33, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 10:06:24 -0500 33, 28 -- X-Authority-Analysis: v=1.1 cv=pLPlkKcK38cN4Cv4YkVeTGGRYpmKez/IrX640LVKWnc= c=1 sm=0 a=nGPnte84VBEA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=tkoM7y6riZj4z8YaxSEA:9 a=ZHt2qSYvVdxlc-6TZLkA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 33, 28 -- X-Cloudmark-Score: 0 33, 28 -- X-Originating-IP: 72.224.149.57 33, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3813] helo=Ken) 33, 28 -- by cdptpa-oedge04.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 33, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 33, 28 -- id D4/E5-20202-FE4020E4; Wed, 22 Jun 2011 15:06:23 +0000 33, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 33, 28 -- To: {Roy-at-picotech.co.il} , "MSA Listserver" {microscopy-at-microscopy.com} 33, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 33, 28 -- Date: Wed, 22 Jun 2011 11:06:10 -0400 33, 28 -- Message-ID: {99C306969A954EAE952E39FD81828717-at-Ken} 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="iso-8859-1" 33, 28 -- X-Priority: 3 (Normal) 33, 28 -- X-MSMail-Priority: Normal 33, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 33, 28 -- In-Reply-To: {201106221431.p5MEVDhm008546-at-ns.microscopy.com} 33, 28 -- Thread-Index: Acww6Qn916P2m7amSB6IsRlktAomswAAqauA 33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 33, 28 -- Importance: Normal 33, 28 -- Content-Transfer-Encoding: 8bit 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MF6NHc030811 ==============================End of - Headers==============================
If you look at the numbers involved in this project, you will see that it will be very tough to find a solution. Your wafer is 6". That's 730 cm^2 or 7.3x10^12 microns^2. If you have, say, a 7 MPixel camera and you could perfectly align the images, you would need on the order of 10,000 images (more if you consider Nyquist, even more if you need overlap). That would get you a 2D image. 20,000 or 30,000 images would take you about 1 hour if you could snap 10 pictures a second. However, you probably have to slow this down for mechanical movement to stabilize when you either move the camera or the wafer. Obviously you don't want to reduce the resolution by compression, so you would end up with 30,000 images, each 21 MB (color, 24 bit), or almost a Terabyte for each wafer. These images would then have to be montaged. And I am not sure how you would get 3D information from your images.
The Medical field has been working on something like this for a while now. There are "slide scanners", which scan a whole medical light microscopy slide and allow the doctors to see it off-line. However, they typically scan an area of 1x1". And depending on the resolution, it can take hours. Nevertheless, you might want to talk to the manufacturers of these systems to find out if there is anything that can be done for the "wafer scanner" you have in mind. One of those companies is Olympus (disclaimer: I used to work for them, but I have no longer any connection to that part of the business), which you can find here: http://www.olympusamerica.com/seg_section/product.asp?product=1087&c=9. There are other companies as well.
--- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: steven.samuelsson-at-sri.com [mailto:steven.samuelsson-at-sri.com] Sent: Thursday, June 09, 2011 11:27 AM To: mike.bode-at-resaltatech.com
Dear Listserve,
Am working with an engineer on a proposal that involves predictive modeling: processes and procedures for silicone wafer etching and design. I have been included because they wish to monitor etchings via some kind of imager. Although this is outside of my level of expertise, the intrigue has brought me into the project. Now I need to discover our options and hope turn-key systems, at affordable prices, are available. There are a number of parameters and restrictions and are listed here.
-object is a 6" silicone wafer; submerged in/out of 5% HF acid with the imaging taking place remote to the bath. Robot would likely pull out the wafer and position on an imager stage (or the equivalent) then return to bath, etc. -most of the 6" disc will need to be imaged; we realize that fields will be tiled together -resolution: ~10um. -capture rate is a few images each hour; maybe more but no video rate. -wish to be able to display image sets in 3-D (much like SEMs) -capital limit at ~$15K
Thanks so much for suggestions.
Steve
-- Steven Samuelsson, PhD Director Cell and Molecular Imaging Facility 100-51 SRI International, Inc. 333 Ravenswood Ave Menlo Park, CA 94061 (650) 859-2980
==============================Original Headers============================== 7, 18 -- From steven.samuelsson-at-sri.com Thu Jun 9 12:11:11 2011 7, 18 -- Received: from mail1.sri.com (newmail.SRI.COM [128.18.30.17]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p59HBB1d003903 7, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 9 Jun 2011 12:11:11 -0500 7, 18 -- MIME-version: 1.0 7, 18 -- Content-transfer-encoding: 7BIT 7, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 7, 18 -- Received: from Steves-iMac.local ([unknown] [128.18.51.104]) 7, 18 -- by mail.sri.com (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built 7, 18 -- Jul 30 2009)) with ESMTP id {0LMJ00LFC92NXL40-at-mail.sri.com} for 7, 18 -- Microscopy-at-microscopy.com; Thu, 09 Jun 2011 10:11:11 -0700 (PDT) 7, 18 -- Message-id: {4DF0FEAF.7080301-at-sri.com} 7, 18 -- Date: Thu, 09 Jun 2011 10:11:11 -0700 7, 18 -- From: Steven Samuelsson {steven.samuelsson-at-sri.com} 7, 18 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.2.17) 7, 18 -- Gecko/20110414 Thunderbird/3.1.10 7, 18 -- To: Microscopy-at-microscopy.com 7, 18 -- Subject: Suggestions for a 3-D Imager ==============================End of - Headers==============================
==============================Original Headers============================== 24, 31 -- From mike.bode-at-resaltatech.com Wed Jun 22 11:24:11 2011 24, 31 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 24, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MGOBtE015772 24, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 11:24:11 -0500 24, 31 -- Received: from c-174-51-0-206.hsd1.co.comcast.net ([174.51.0.206]:16932 helo=resdtpden01) 24, 31 -- by web38.justhost.com with esmtpa (Exim 4.69) 24, 31 -- (envelope-from {mike.bode-at-resaltatech.com} ) 24, 31 -- id 1QZQDu-00043U-AW; Wed, 22 Jun 2011 11:24:14 -0500 24, 31 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 24, 31 -- To: {Microscopy-at-microscopy.com} 24, 31 -- Cc: {steven.samuelsson-at-sri.com} 24, 31 -- References: {201106091727.p59HR6YL017441-at-ns.microscopy.com} 24, 31 -- In-Reply-To: {201106091727.p59HR6YL017441-at-ns.microscopy.com} 24, 31 -- Subject: RE: [Microscopy] Suggestions for a 3-D Imager 24, 31 -- Date: Wed, 22 Jun 2011 10:24:05 -0600 24, 31 -- Message-ID: {006501cc30f8$cf996120$6ecc2360$-at-bode-at-resaltatech.com} 24, 31 -- MIME-Version: 1.0 24, 31 -- Content-Type: text/plain; 24, 31 -- charset="us-ascii" 24, 31 -- Content-Transfer-Encoding: 7bit 24, 31 -- X-Mailer: Microsoft Office Outlook 12.0 24, 31 -- Content-Language: en-us 24, 31 -- Thread-Index: AcwmyncUnPVECTfrSv+09KqRF9jvlgKLBdaw 24, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 24, 31 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 24, 31 -- X-AntiAbuse: Original Domain - microscopy.com 24, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 24, 31 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 24, 31 -- X-Source: 24, 31 -- X-Source-Args: 24, 31 -- X-Source-Dir: ==============================End of - Headers==============================
I usually agree with your comments but this time I have to throw in a curved ball.
I often work with automated analysis SEMs which have a tungsten filament life of upwards from 2500 hours; admittedly we run at 11 micro amps emission with the filament well back. The instrument are not used by electron microscopists so the idea behind this long life being that the service technician is able to fit a new filament on their six monthly service visits.
We worked hard to obtain this life and one of the features that we found that was very important in our development was the vacuum level. Thus we run with an ion pump on the gun and the difference in filament life is considerable. This is no criticism of the basic instrument, which exhibits a gun vacuum level in its standard form the same if not better than its competitors.
Another area that the gun vacuum level influenced was the high voltage stability. The instruments need to run for up to 24 hours unattended and it is most important for the high voltage to remain absolutely stable over that entire period of time. Double plots of high voltage level and gun vacuum show that once the gun vacuum settles so does the high voltage. Thus the operators wait for this stability period prior to starting a sample run; proven to be the best practice.
As a second point I know LaB6 users who have been forced to use tungsten whilst waiting for a new source and they have also commented on the increased filament life.
Maybe I should say that not one of these comments relates to clients in the United States; must be related to the type of air :-)
Regards
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: 22 June 2011 16:07 To: protrain-at-emcourses.com
Roy, I beg to differ. I have not seen any significant increase in tungsten filament life in an ion-pumped gun versus a normal gun in good condition in an SEM. Poor vacuum will definitely shorten a W filament's life, but a better vacuum makes very little difference due to the fact that W filaments run hotter than LaB6 and evaporate faster. The reason poor vacuum accelerates their demise is due to oxidation on top of evaporation.
No W filament I've ever seen has lasted more than about 300 hours at operating temperature in an SEM, 10E-7T vacuum notwithstanding. My observations may not hold for a TEM because TEMs run at lower temperatures/emission currents and therefore evaporation rates should be significantly lower.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] Sent: Wednesday, June 22, 2011 10:31 AM To: kenconverse-at-qualityimages.biz
Hi John.
Like Nicolas said here below, it's all a matter of vacuum. Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs usually have a filament life of way above 1000 hours. If you take the same SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get way above 1000 hours with the tungsten filament.
So, in theory if your SEM gun has a very good vacuum, you should be getting a very high lifetime from each tungsten filament. If not, you could expect to get good result with the LaB6 filemant, but keep the lifetime of your current tungsten one. Keep in mind that the LaB6 filament is exponentially more expensive than tungsten.
It'd be best to consult with Jeol in any case before you decide to do this.
Regards, Roy
-----Original Message----- X-from: Nicolas.Stephant-at-univ-nantes.fr [mailto:Nicolas.Stephant-at-univ-nantes.fr] Sent: Thursday, June 16, 2011 1:45 PM To: Roy Golombick
Hi John
LaB6 filament have to work in a good vacuum. There is some instruments like your TEM working with LaB6 in a gun pumped by DP or turbo, but most of the LaB6 TEM work with high vacuum in the gun provided by SIP. If your vacuum system is very good you may use LaB6 but you must understand that you will work on an edge. Anyway, you may try without danger for your instrument and you will see if LaB6 stay save during one year (good) or one month. Please note that you may use another wehnelt cap for this kind of filament and you have to change distance beetween filament tip and wehnelt cap. First eating of this filament must be done very slowy (about one hour) after almost one night of pumping. Working with LaB6 is very comfortable because the brigtness is very high and it may increase significantly top end result. Regards
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
--
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GAbEpl020489 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5GAb9nv007050; 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5GAb7iQ000928 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Ste phant-at-univ-nantes.fr} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From Roy-at-picotech.co.il Wed Jun 22 09:29:42 2011 21, 25 -- Received: from mail.picotech.co.il (mail.picotech.co.il [212.143.255.226]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5METfrh003527 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 09:29:41 -0500 21, 25 -- Received: from SERVER01.picotech.local ([192.168.1.2]) by server01 21, 25 -- ([192.168.1.2]) with mapi; Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- From: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 25 -- Disposition-Notification-To: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- Date: Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Topic: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Index: AcwsEjA2FcR8LNxjSzquPhESHEjIbQE1a7nA 21, 25 -- Message-ID: {ABB156E109DC9A42B8109368CA6F4895178F899703-at-server01} 21, 25 -- References: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- In-Reply-To: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- Accept-Language: en-US 21, 25 -- Content-Language: en-US 21, 25 -- X-MS-Has-Attach: 21, 25 -- X-MS-TNEF-Correlator: 21, 25 -- acceptlanguage: en-US 21, 25 -- Content-Type: text/plain; charset="iso-8859-1" 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5METfrh003527 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 10:06:24 2011 33, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MF6NHc030811 33, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 10:06:24 -0500 33, 28 -- X-Authority-Analysis: v=1.1 cv=pLPlkKcK38cN4Cv4YkVeTGGRYpmKez/IrX640LVKWnc= c=1 sm=0 a=nGPnte84VBEA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=tkoM7y6riZj4z8YaxSEA:9 a=ZHt2qSYvVdxlc-6TZLkA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 33, 28 -- X-Cloudmark-Score: 0 33, 28 -- X-Originating-IP: 72.224.149.57 33, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3813] helo=Ken) 33, 28 -- by cdptpa-oedge04.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 33, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 33, 28 -- id D4/E5-20202-FE4020E4; Wed, 22 Jun 2011 15:06:23 +0000 33, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 33, 28 -- To: {Roy-at-picotech.co.il} , "MSA Listserver" {microscopy-at-microscopy.com} 33, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 33, 28 -- Date: Wed, 22 Jun 2011 11:06:10 -0400 33, 28 -- Message-ID: {99C306969A954EAE952E39FD81828717-at-Ken} 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="iso-8859-1" 33, 28 -- X-Priority: 3 (Normal) 33, 28 -- X-MSMail-Priority: Normal 33, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 33, 28 -- In-Reply-To: {201106221431.p5MEVDhm008546-at-ns.microscopy.com} 33, 28 -- Thread-Index: Acww6Qn916P2m7amSB6IsRlktAomswAAqauA 33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 33, 28 -- Importance: Normal 33, 28 -- Content-Transfer-Encoding: 8bit 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MF6NHc030811 ==============================End of - Headers==============================
==============================Original Headers============================== 52, 28 -- From protrain-at-emcourses.com Wed Jun 22 11:50:32 2011 52, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 52, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MGoU41032318 52, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 11:50:31 -0500 52, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:51659 helo=ProtrainPC) 52, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 52, 28 -- (envelope-from {protrain-at-emcourses.com} ) 52, 28 -- id 1QZQdG-000Loz-0E by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 22 Jun 2011 18:50:27 +0200 52, 28 -- Reply-To: {protrain-at-emcourses.com} 52, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 52, 28 -- To: {kenconverse-at-qualityimages.biz} 52, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 52, 28 -- References: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} 52, 28 -- In-Reply-To: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} 52, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 52, 28 -- Date: Wed, 22 Jun 2011 17:49:52 +0100 52, 28 -- Organization: Protrain 52, 28 -- Message-ID: {004d01cc30fc$7b2a66d0$717f3470$-at-com} 52, 28 -- MIME-Version: 1.0 52, 28 -- Content-Type: text/plain; 52, 28 -- charset="iso-8859-1" 52, 28 -- X-Mailer: Microsoft Office Outlook 12.0 52, 28 -- Thread-Index: Acww7ieAAtwiidP2TCyhjyuu2/R/AQABzfdA 52, 28 -- Content-Language: en-gb 52, 28 -- X-Scan-Signature: c7f1ef588874116c1f795ee3e363243c{443}} 52, 28 -- X-Trace: smtp01.dial-up.net 1QZQdG-000Loz-0E 53857f849a2877cf96ab3a0e288b1a64 52, 28 -- Content-Transfer-Encoding: 8bit 52, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MGoU41032318 ==============================End of - Headers==============================
Hi Steve, The air, the air... oh, you mean all the hot air? You might have a point ;-)
The 11 uA emission current leads me to think that the lower temperature enhances the effects of good vacuum. As for a more normal system, I've found Amrays follow the manual, 40 hrs, ion pump or not, unless the vacuum is especially poor. JEOLs seem about the same except their typical life is 100-200 hours. I did have one dry pumped ETEC that would run for weeks and months 24-7 in the 10e-8T range, but it was run slightly undersaturated because life was more important than stability for the particular experiment.
Maybe you can shed some light on this thought. I've always wondered how much effect the physical gun design has on the temperature required for a particular emission current. Your example seems to indicate the same thing that TEMs seem to indicate and that is that the lower emission currents, and consequent lower temperatures result in oxidation being a larger factor than evaporation on filament life, so better vacuum has a greater effect on lower temperature filaments. Any thoughts?
Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Wednesday, June 22, 2011 12:52 PM To: kenconverse-at-qualityimages.biz
Hi Ken
I usually agree with your comments but this time I have to throw in a curved ball.
I often work with automated analysis SEMs which have a tungsten filament life of upwards from 2500 hours; admittedly we run at 11 micro amps emission with the filament well back. The instrument are not used by electron microscopists so the idea behind this long life being that the service technician is able to fit a new filament on their six monthly service visits.
We worked hard to obtain this life and one of the features that we found that was very important in our development was the vacuum level. Thus we run with an ion pump on the gun and the difference in filament life is considerable. This is no criticism of the basic instrument, which exhibits a gun vacuum level in its standard form the same if not better than its competitors.
Another area that the gun vacuum level influenced was the high voltage stability. The instruments need to run for up to 24 hours unattended and it is most important for the high voltage to remain absolutely stable over that entire period of time. Double plots of high voltage level and gun vacuum show that once the gun vacuum settles so does the high voltage. Thus the operators wait for this stability period prior to starting a sample run; proven to be the best practice.
As a second point I know LaB6 users who have been forced to use tungsten whilst waiting for a new source and they have also commented on the increased filament life.
Maybe I should say that not one of these comments relates to clients in the United States; must be related to the type of air :-)
Regards
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Sent: 22 June 2011 16:07 To: protrain-at-emcourses.com
Roy, I beg to differ. I have not seen any significant increase in tungsten filament life in an ion-pumped gun versus a normal gun in good condition in an SEM. Poor vacuum will definitely shorten a W filament's life, but a better vacuum makes very little difference due to the fact that W filaments run hotter than LaB6 and evaporate faster. The reason poor vacuum accelerates their demise is due to oxidation on top of evaporation.
No W filament I've ever seen has lasted more than about 300 hours at operating temperature in an SEM, 10E-7T vacuum notwithstanding. My observations may not hold for a TEM because TEMs run at lower temperatures/emission currents and therefore evaporation rates should be significantly lower.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] Sent: Wednesday, June 22, 2011 10:31 AM To: kenconverse-at-qualityimages.biz
Hi John.
Like Nicolas said here below, it's all a matter of vacuum. Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs usually have a filament life of way above 1000 hours. If you take the same SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get way above 1000 hours with the tungsten filament.
So, in theory if your SEM gun has a very good vacuum, you should be getting a very high lifetime from each tungsten filament. If not, you could expect to get good result with the LaB6 filemant, but keep the lifetime of your current tungsten one. Keep in mind that the LaB6 filament is exponentially more expensive than tungsten.
It'd be best to consult with Jeol in any case before you decide to do this.
Regards, Roy
-----Original Message----- X-from: Nicolas.Stephant-at-univ-nantes.fr [mailto:Nicolas.Stephant-at-univ-nantes.fr] Sent: Thursday, June 16, 2011 1:45 PM To: Roy Golombick
Hi John
LaB6 filament have to work in a good vacuum. There is some instruments like your TEM working with LaB6 in a gun pumped by DP or turbo, but most of the LaB6 TEM work with high vacuum in the gun provided by SIP. If your vacuum system is very good you may use LaB6 but you must understand that you will work on an edge. Anyway, you may try without danger for your instrument and you will see if LaB6 stay save during one year (good) or one month. Please note that you may use another wehnelt cap for this kind of filament and you have to change distance beetween filament tip and wehnelt cap. First eating of this filament must be done very slowy (about one hour) after almost one night of pumping. Working with LaB6 is very comfortable because the brigtness is very high and it may increase significantly top end result. Regards
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
--
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GAbEpl020489 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5GAb9nv007050; 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5GAb7iQ000928 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Ste phant-at-univ-nantes.fr} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From Roy-at-picotech.co.il Wed Jun 22 09:29:42 2011 21, 25 -- Received: from mail.picotech.co.il (mail.picotech.co.il [212.143.255.226]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5METfrh003527 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 09:29:41 -0500 21, 25 -- Received: from SERVER01.picotech.local ([192.168.1.2]) by server01 21, 25 -- ([192.168.1.2]) with mapi; Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- From: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 25 -- Disposition-Notification-To: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- Date: Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Topic: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Index: AcwsEjA2FcR8LNxjSzquPhESHEjIbQE1a7nA 21, 25 -- Message-ID: {ABB156E109DC9A42B8109368CA6F4895178F899703-at-server01} 21, 25 -- References: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- In-Reply-To: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- Accept-Language: en-US 21, 25 -- Content-Language: en-US 21, 25 -- X-MS-Has-Attach: 21, 25 -- X-MS-TNEF-Correlator: 21, 25 -- acceptlanguage: en-US 21, 25 -- Content-Type: text/plain; charset="iso-8859-1" 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5METfrh003527 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 10:06:24 2011 33, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MF6NHc030811 33, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 10:06:24 -0500 33, 28 -- X-Authority-Analysis: v=1.1 cv=pLPlkKcK38cN4Cv4YkVeTGGRYpmKez/IrX640LVKWnc= c=1 sm=0 a=nGPnte84VBEA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=tkoM7y6riZj4z8YaxSEA:9 a=ZHt2qSYvVdxlc-6TZLkA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 33, 28 -- X-Cloudmark-Score: 0 33, 28 -- X-Originating-IP: 72.224.149.57 33, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3813] helo=Ken) 33, 28 -- by cdptpa-oedge04.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 33, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 33, 28 -- id D4/E5-20202-FE4020E4; Wed, 22 Jun 2011 15:06:23 +0000 33, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 33, 28 -- To: {Roy-at-picotech.co.il} , "MSA Listserver" {microscopy-at-microscopy.com} 33, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 33, 28 -- Date: Wed, 22 Jun 2011 11:06:10 -0400 33, 28 -- Message-ID: {99C306969A954EAE952E39FD81828717-at-Ken} 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="iso-8859-1" 33, 28 -- X-Priority: 3 (Normal) 33, 28 -- X-MSMail-Priority: Normal 33, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 33, 28 -- In-Reply-To: {201106221431.p5MEVDhm008546-at-ns.microscopy.com} 33, 28 -- Thread-Index: Acww6Qn916P2m7amSB6IsRlktAomswAAqauA 33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 33, 28 -- Importance: Normal 33, 28 -- Content-Transfer-Encoding: 8bit 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MF6NHc030811 ==============================End of - Headers==============================
==============================Original Headers============================== 52, 28 -- From protrain-at-emcourses.com Wed Jun 22 11:50:32 2011 52, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 52, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MGoU41032318 52, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 11:50:31 -0500 52, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:51659 helo=ProtrainPC) 52, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 52, 28 -- (envelope-from {protrain-at-emcourses.com} ) 52, 28 -- id 1QZQdG-000Loz-0E by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 22 Jun 2011 18:50:27 +0200 52, 28 -- Reply-To: {protrain-at-emcourses.com} 52, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 52, 28 -- To: {kenconverse-at-qualityimages.biz} 52, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 52, 28 -- References: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} 52, 28 -- In-Reply-To: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} 52, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 52, 28 -- Date: Wed, 22 Jun 2011 17:49:52 +0100 52, 28 -- Organization: Protrain 52, 28 -- Message-ID: {004d01cc30fc$7b2a66d0$717f3470$-at-com} 52, 28 -- MIME-Version: 1.0 52, 28 -- Content-Type: text/plain; 52, 28 -- charset="iso-8859-1" 52, 28 -- X-Mailer: Microsoft Office Outlook 12.0 52, 28 -- Thread-Index: Acww7ieAAtwiidP2TCyhjyuu2/R/AQABzfdA 52, 28 -- Content-Language: en-gb 52, 28 -- X-Scan-Signature: c7f1ef588874116c1f795ee3e363243c{443}} 52, 28 -- X-Trace: smtp01.dial-up.net 1QZQdG-000Loz-0E 53857f849a2877cf96ab3a0e288b1a64 52, 28 -- Content-Transfer-Encoding: 8bit 52, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MGoU41032318 ==============================End of - Headers==============================
==============================Original Headers============================== 66, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 12:33:48 2011 66, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 66, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MHXmOw018481 66, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 12:33:48 -0500 66, 28 -- X-Authority-Analysis: v=1.1 cv=aeMH4JcVOnVr0LmJAzqEvfnmJyuaZufWdlng4HTRGCk= c=1 sm=0 a=nGPnte84VBEA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=TjS3GnXOAAAA:8 a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=ayC55rCoAAAA:8 a=h_vVgvW_AAAA:8 a=E0F6PzzqAAAA:8 a=5qZVn9eiuOZf3ur7tZcA:9 a=pz22PO0dAz2PExYwF1kA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=a7qs6diK2AgA:10 a=RyjLG6jaqdoA:10 a=4NIop_tvI_gA:10 a=3_2eAsHTqJoA:10 a=18pRNVDt_m0A:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 66, 28 -- X-Cloudmark-Score: 0 66, 28 -- X-Originating-IP: 72.224.149.57 66, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1109] helo=Ken) 66, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 66, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 66, 28 -- id F9/A2-03893-B77220E4; Wed, 22 Jun 2011 17:33:48 +0000 66, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 66, 28 -- To: {protrain-at-emcourses.com} , "MSA Listserver" {microscopy-at-microscopy.com} 66, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 66, 28 -- Date: Wed, 22 Jun 2011 13:33:34 -0400 66, 28 -- Message-ID: {645DAD6306BA4DD9AD55BD210C83BA7A-at-Ken} 66, 28 -- MIME-Version: 1.0 66, 28 -- Content-Type: text/plain; 66, 28 -- charset="iso-8859-1" 66, 28 -- X-Priority: 3 (Normal) 66, 28 -- X-MSMail-Priority: Normal 66, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 66, 28 -- In-Reply-To: {201106221652.p5MGqBWY003344-at-ns.microscopy.com} 66, 28 -- Thread-Index: Acww/LtZV9um7gK0RnWik8TGADTktQAA2pnQ 66, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 66, 28 -- Importance: Normal 66, 28 -- Content-Transfer-Encoding: 8bit 66, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MHXmOw018481 ==============================End of - Headers==============================
I seem to have spent most of my life playing (!) with electron guns. How often I have moved filaments forward or backwards, sharpened tungsten filaments to obtain LaB6 type performance, lifted up anodes and checked out different cathode shapes!
To most people in laboratories a gun is what the manufacturer gives you, but with the good old tungsten hairpin that may be considered to be just a starting point. Total cathode geometry and the changes that you are able to make regarding filament life in one direction and performance in the other, are outside of the norm. Add to this raising the anode to improve performance and few appreciate how you may transform an instrument.
On tungsten filament temperature I have a great deal to say but as usual from me it is probably controversial? I rarely fully saturate the gun! I am a great believer in under saturating. I see no point in throwing away filament life and with modern stabilities there is usually no point in doing so. An amazing amount of work may be accomplished and for most SEM analysis there is no point, it hardly can be said to improve x-ray resolution!
I only saturate when working at the absolute limit at a particular kV and I challenge anyone to see a visual degradation in any images I record through using this technique; but only up to 100,000X. The big bonus for most labs is this technique really does improve tungsten or even LaB6 filament life.
Thus I agree with you with low filament currents it is oxidation that is the final tungsten filament killer not evaporation! Too hot a filament and it just evaporates but be more thoughtful and life is extended. We improve the vacuum and the life is extended even further; but probably at the cost ($) of an ion pump!
Regards
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
---Original Message----- X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] Sent: 22 June 2011 18:35 To: protrain-at-emcourses.com
Hi Steve, The air, the air... oh, you mean all the hot air? You might have a point ;-)
The 11 uA emission current leads me to think that the lower temperature enhances the effects of good vacuum. As for a more normal system, I've found Amrays follow the manual, 40 hrs, ion pump or not, unless the vacuum is especially poor. JEOLs seem about the same except their typical life is 100-200 hours. I did have one dry pumped ETEC that would run for weeks and months 24-7 in the 10e-8T range, but it was run slightly undersaturated because life was more important than stability for the particular experiment.
Maybe you can shed some light on this thought. I've always wondered how much effect the physical gun design has on the temperature required for a particular emission current. Your example seems to indicate the same thing that TEMs seem to indicate and that is that the lower emission currents, and consequent lower temperatures result in oxidation being a larger factor than evaporation on filament life, so better vacuum has a greater effect on lower temperature filaments. Any thoughts?
Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Wednesday, June 22, 2011 12:52 PM To: kenconverse-at-qualityimages.biz
Hi Ken
I usually agree with your comments but this time I have to throw in a curved ball.
I often work with automated analysis SEMs which have a tungsten filament life of upwards from 2500 hours; admittedly we run at 11 micro amps emission with the filament well back. The instrument are not used by electron microscopists so the idea behind this long life being that the service technician is able to fit a new filament on their six monthly service visits.
We worked hard to obtain this life and one of the features that we found that was very important in our development was the vacuum level. Thus we run with an ion pump on the gun and the difference in filament life is considerable. This is no criticism of the basic instrument, which exhibits a gun vacuum level in its standard form the same if not better than its competitors.
Another area that the gun vacuum level influenced was the high voltage stability. The instruments need to run for up to 24 hours unattended and it is most important for the high voltage to remain absolutely stable over that entire period of time. Double plots of high voltage level and gun vacuum show that once the gun vacuum settles so does the high voltage. Thus the operators wait for this stability period prior to starting a sample run; proven to be the best practice.
As a second point I know LaB6 users who have been forced to use tungsten whilst waiting for a new source and they have also commented on the increased filament life.
Maybe I should say that not one of these comments relates to clients in the United States; must be related to the type of air :-)
Regards
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Sent: 22 June 2011 16:07 To: protrain-at-emcourses.com
Roy, I beg to differ. I have not seen any significant increase in tungsten filament life in an ion-pumped gun versus a normal gun in good condition in an SEM. Poor vacuum will definitely shorten a W filament's life, but a better vacuum makes very little difference due to the fact that W filaments run hotter than LaB6 and evaporate faster. The reason poor vacuum accelerates their demise is due to oxidation on top of evaporation.
No W filament I've ever seen has lasted more than about 300 hours at operating temperature in an SEM, 10E-7T vacuum notwithstanding. My observations may not hold for a TEM because TEMs run at lower temperatures/emission currents and therefore evaporation rates should be significantly lower.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] Sent: Wednesday, June 22, 2011 10:31 AM To: kenconverse-at-qualityimages.biz
Hi John.
Like Nicolas said here below, it's all a matter of vacuum. Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs usually have a filament life of way above 1000 hours. If you take the same SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get way above 1000 hours with the tungsten filament.
So, in theory if your SEM gun has a very good vacuum, you should be getting a very high lifetime from each tungsten filament. If not, you could expect to get good result with the LaB6 filemant, but keep the lifetime of your current tungsten one. Keep in mind that the LaB6 filament is exponentially more expensive than tungsten.
It'd be best to consult with Jeol in any case before you decide to do this.
Regards, Roy
-----Original Message----- X-from: Nicolas.Stephant-at-univ-nantes.fr [mailto:Nicolas.Stephant-at-univ-nantes.fr] Sent: Thursday, June 16, 2011 1:45 PM To: Roy Golombick
Hi John
LaB6 filament have to work in a good vacuum. There is some instruments like your TEM working with LaB6 in a gun pumped by DP or turbo, but most of the LaB6 TEM work with high vacuum in the gun provided by SIP. If your vacuum system is very good you may use LaB6 but you must understand that you will work on an edge. Anyway, you may try without danger for your instrument and you will see if LaB6 stay save during one year (good) or one month. Please note that you may use another wehnelt cap for this kind of filament and you have to change distance beetween filament tip and wehnelt cap. First eating of this filament must be done very slowy (about one hour) after almost one night of pumping. Working with LaB6 is very comfortable because the brigtness is very high and it may increase significantly top end result. Regards
Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung john.mitchels-at-gmail.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from anyone } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } The microscope is behaving itself very well, no leaks everything is as } good as it gets given its age. With one fitted do you notice any } significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original Headers============================== } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5G8RUwL002495 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 -0500 } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=domainkey-signature:mime-version:date:message-id:subject:from:to } 4, 30 -- :content-type; } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } 4, 30 -- b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } 4, 30 -- fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } 4, 30 -- d=gmail.com; s=gamma; } 4, 30 -- h=mime-version:date:message-id:subject:from:to:content-type; } 4, 30 -- b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } 4, 30 -- fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } 4, 30 -- MIME-Version: 1.0 } 4, 30 -- Received: by 10.68.51.201 with SMTP id m9mr351917pbo.135.1308212849644; Thu, } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 -0700 (PDT) } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } 4, 30 -- To: Microscopy-at-microscopy.com } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== }
--
==============================Original Headers============================== 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5GAbEpl020489 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 -0500 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5GAb9nv007050; 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5GAb7iQ000928 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 8, 25 -- MIME-Version: 1.0 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 8, 25 -- X-j-chkmail-Enveloppe: 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Ste phant-at-univ-nantes.fr} ==============================End of - Headers==============================
==============================Original Headers============================== 21, 25 -- From Roy-at-picotech.co.il Wed Jun 22 09:29:42 2011 21, 25 -- Received: from mail.picotech.co.il (mail.picotech.co.il [212.143.255.226]) 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5METfrh003527 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 09:29:41 -0500 21, 25 -- Received: from SERVER01.picotech.local ([192.168.1.2]) by server01 21, 25 -- ([192.168.1.2]) with mapi; Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- From: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 21, 25 -- Disposition-Notification-To: Roy Golombick {Roy-at-picotech.co.il} 21, 25 -- Date: Wed, 22 Jun 2011 17:27:59 +0300 21, 25 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Topic: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 21, 25 -- Thread-Index: AcwsEjA2FcR8LNxjSzquPhESHEjIbQE1a7nA 21, 25 -- Message-ID: {ABB156E109DC9A42B8109368CA6F4895178F899703-at-server01} 21, 25 -- References: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- In-Reply-To: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} 21, 25 -- Accept-Language: en-US 21, 25 -- Content-Language: en-US 21, 25 -- X-MS-Has-Attach: 21, 25 -- X-MS-TNEF-Correlator: 21, 25 -- acceptlanguage: en-US 21, 25 -- Content-Type: text/plain; charset="iso-8859-1" 21, 25 -- MIME-Version: 1.0 21, 25 -- Content-Transfer-Encoding: 8bit 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5METfrh003527 ==============================End of - Headers==============================
==============================Original Headers============================== 33, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 10:06:24 2011 33, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MF6NHc030811 33, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 10:06:24 -0500 33, 28 -- X-Authority-Analysis: v=1.1 cv=pLPlkKcK38cN4Cv4YkVeTGGRYpmKez/IrX640LVKWnc= c=1 sm=0 a=nGPnte84VBEA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=tkoM7y6riZj4z8YaxSEA:9 a=ZHt2qSYvVdxlc-6TZLkA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 33, 28 -- X-Cloudmark-Score: 0 33, 28 -- X-Originating-IP: 72.224.149.57 33, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3813] helo=Ken) 33, 28 -- by cdptpa-oedge04.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 33, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 33, 28 -- id D4/E5-20202-FE4020E4; Wed, 22 Jun 2011 15:06:23 +0000 33, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 33, 28 -- To: {Roy-at-picotech.co.il} , "MSA Listserver" {microscopy-at-microscopy.com} 33, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 33, 28 -- Date: Wed, 22 Jun 2011 11:06:10 -0400 33, 28 -- Message-ID: {99C306969A954EAE952E39FD81828717-at-Ken} 33, 28 -- MIME-Version: 1.0 33, 28 -- Content-Type: text/plain; 33, 28 -- charset="iso-8859-1" 33, 28 -- X-Priority: 3 (Normal) 33, 28 -- X-MSMail-Priority: Normal 33, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 33, 28 -- In-Reply-To: {201106221431.p5MEVDhm008546-at-ns.microscopy.com} 33, 28 -- Thread-Index: Acww6Qn916P2m7amSB6IsRlktAomswAAqauA 33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 33, 28 -- Importance: Normal 33, 28 -- Content-Transfer-Encoding: 8bit 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MF6NHc030811 ==============================End of - Headers==============================
==============================Original Headers============================== 52, 28 -- From protrain-at-emcourses.com Wed Jun 22 11:50:32 2011 52, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 52, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MGoU41032318 52, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 11:50:31 -0500 52, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:51659 helo=ProtrainPC) 52, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 52, 28 -- (envelope-from {protrain-at-emcourses.com} ) 52, 28 -- id 1QZQdG-000Loz-0E by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 22 Jun 2011 18:50:27 +0200 52, 28 -- Reply-To: {protrain-at-emcourses.com} 52, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 52, 28 -- To: {kenconverse-at-qualityimages.biz} 52, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 52, 28 -- References: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} 52, 28 -- In-Reply-To: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} 52, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 52, 28 -- Date: Wed, 22 Jun 2011 17:49:52 +0100 52, 28 -- Organization: Protrain 52, 28 -- Message-ID: {004d01cc30fc$7b2a66d0$717f3470$-at-com} 52, 28 -- MIME-Version: 1.0 52, 28 -- Content-Type: text/plain; 52, 28 -- charset="iso-8859-1" 52, 28 -- X-Mailer: Microsoft Office Outlook 12.0 52, 28 -- Thread-Index: Acww7ieAAtwiidP2TCyhjyuu2/R/AQABzfdA 52, 28 -- Content-Language: en-gb 52, 28 -- X-Scan-Signature: c7f1ef588874116c1f795ee3e363243c{443}} 52, 28 -- X-Trace: smtp01.dial-up.net 1QZQdG-000Loz-0E 53857f849a2877cf96ab3a0e288b1a64 52, 28 -- Content-Transfer-Encoding: 8bit 52, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MGoU41032318 ==============================End of - Headers==============================
==============================Original Headers============================== 66, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 12:33:48 2011 66, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 66, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MHXmOw018481 66, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 12:33:48 -0500 66, 28 -- X-Authority-Analysis: v=1.1 cv=aeMH4JcVOnVr0LmJAzqEvfnmJyuaZufWdlng4HTRGCk= c=1 sm=0 a=nGPnte84VBEA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=TjS3GnXOAAAA:8 a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=ayC55rCoAAAA:8 a=h_vVgvW_AAAA:8 a=E0F6PzzqAAAA:8 a=5qZVn9eiuOZf3ur7tZcA:9 a=pz22PO0dAz2PExYwF1kA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=a7qs6diK2AgA:10 a=RyjLG6jaqdoA:10 a=4NIop_tvI_gA:10 a=3_2eAsHTqJoA:10 a=18pRNVDt_m0A:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 66, 28 -- X-Cloudmark-Score: 0 66, 28 -- X-Originating-IP: 72.224.149.57 66, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1109] helo=Ken) 66, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 66, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 66, 28 -- id F9/A2-03893-B77220E4; Wed, 22 Jun 2011 17:33:48 +0000 66, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 66, 28 -- To: {protrain-at-emcourses.com} , "MSA Listserver" {microscopy-at-microscopy.com} 66, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 66, 28 -- Date: Wed, 22 Jun 2011 13:33:34 -0400 66, 28 -- Message-ID: {645DAD6306BA4DD9AD55BD210C83BA7A-at-Ken} 66, 28 -- MIME-Version: 1.0 66, 28 -- Content-Type: text/plain; 66, 28 -- charset="iso-8859-1" 66, 28 -- X-Priority: 3 (Normal) 66, 28 -- X-MSMail-Priority: Normal 66, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 66, 28 -- In-Reply-To: {201106221652.p5MGqBWY003344-at-ns.microscopy.com} 66, 28 -- Thread-Index: Acww/LtZV9um7gK0RnWik8TGADTktQAA2pnQ 66, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 66, 28 -- Importance: Normal 66, 28 -- Content-Transfer-Encoding: 8bit 66, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MHXmOw018481 ==============================End of - Headers==============================
==============================Original Headers============================== 85, 28 -- From protrain-at-emcourses.com Wed Jun 22 14:31:29 2011 85, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 85, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MJVRiF010165 85, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 14:31:27 -0500 85, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:53191 helo=ProtrainPC) 85, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 85, 28 -- (envelope-from {protrain-at-emcourses.com} ) 85, 28 -- id 1QZT91-00009r-QT by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 22 Jun 2011 21:31:25 +0200 85, 28 -- Reply-To: {protrain-at-emcourses.com} 85, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 85, 28 -- To: {kenconverse-at-qualityimages.biz} 85, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 85, 28 -- References: {201106221734.p5MHYhsW019899-at-ns.microscopy.com} 85, 28 -- In-Reply-To: {201106221734.p5MHYhsW019899-at-ns.microscopy.com} 85, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 85, 28 -- Date: Wed, 22 Jun 2011 20:31:18 +0100 85, 28 -- Organization: Protrain 85, 28 -- Message-ID: {000601cc3112$f7af60a0$e70e21e0$-at-com} 85, 28 -- MIME-Version: 1.0 85, 28 -- Content-Type: text/plain; 85, 28 -- charset="iso-8859-1" 85, 28 -- X-Mailer: Microsoft Office Outlook 12.0 85, 28 -- Thread-Index: AcwxArmSvc7TTHkSRlOQmywslhoTnAADB+mQ 85, 28 -- Content-Language: en-gb 85, 28 -- X-Scan-Signature: 60128edfd7223a9476a9143ebe930403{696}} 85, 28 -- X-Trace: smtp01.dial-up.net 1QZT91-00009r-QT 48489186918435a5fb73c649123431f3 85, 28 -- Content-Transfer-Encoding: 8bit 85, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MJVRiF010165 ==============================End of - Headers==============================
I have a fresh 1% tannic acid in acetone solution and I am wondering if I can store it for future experiments.
My questions are: 1. What is the lifetime of a tannic acid solution (days, months or even years)? 2. How is it best stored? (Temperature?, in glass?) 3. Is there an optimal storage concentration (1%, 5%, 20%)?
If anyone has any insight about this, I would appreciate it very much! Thank-you kindly Erin ________________________________
Erin Tranfield PhD Cell Biology and Biophysics Unit European Molecular Biology Laboratory Meyerhofstrasse 1 69117 Heidelberg, Germany T +49 6221 3878620
==============================Original Headers============================== 9, 21 -- From erin.tranfield-at-embl.de Wed Jun 22 14:37:55 2011 9, 21 -- Received: from ironport1.embl.de (ironport1.embl.de [194.94.44.224]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MJbsVk018112 9, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 14:37:54 -0500 9, 21 -- X-IronPort-AV: E=Sophos;i="4.65,407,1304287200"; 9, 21 -- d="scan'208";a="5402806" 9, 21 -- Received: from lxmail01-vm.embl.de (HELO mail.embl.de) ([10.1.1.220]) 9, 21 -- by ironport1.embl.de with ESMTP; 22 Jun 2011 21:37:54 +0200 9, 21 -- Received: (qmail 3997 invoked by uid 107); 22 Jun 2011 19:37:54 -0000 9, 21 -- Received: from unknown (HELO ?192.168.1.6?) (tranfiel-at-78.43.174.182) 9, 21 -- by lxmail01-vm.embl.de with ESMTPA; 22 Jun 2011 19:37:54 -0000 9, 21 -- From: Erin Tranfield {erin.tranfield-at-embl.de} 9, 21 -- Content-Type: text/plain; charset=us-ascii 9, 21 -- Subject: TEM - Lifetime of 1% Tannic Acid Solution? 9, 21 -- Date: Wed, 22 Jun 2011 21:37:53 +0200 9, 21 -- Message-Id: {D4C46511-E10A-488E-AEC2-03979C232542-at-embl.de} 9, 21 -- To: Microscopy-at-microscopy.com 9, 21 -- Mime-Version: 1.0 (Apple Message framework v1084) 9, 21 -- X-Mailer: Apple Mail (2.1084) 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MJbsVk018112 ==============================End of - Headers==============================
OK. The key issue is: "is the vacuum good enough?" If you have an ion pump on the gun and it is operating, then chances are that the vacuum IS good enough. So, all that remains is to buy a filament and try it. JEOL will tell you the best operating conditions for any given make of LaB6 filament. Cost in the US would be up to about $1000, Euros, probably 800 or so. If you can afford that much of a gamble then I would just go for it. You might be able to get longish life out of a tungsten filament, but the LaB6 should be about three orders of magnitude brighter (if my rapidly failing memory serves me correctly). Careful bias and heating should allow 1800 hours plus of use. I choose this figure as it was one of the better numbers we got out of a filament. Remember, we operate in a multi-user, multi-skill-level environment (three to five dozen users) and so I think that is quite remarkable.
PS, We like Denka filaments, we don't have an interest in them but they have pretty much given us good service over the years.
On Jun 22, 2011, at 3:42 PM, protrain-at-emcourses.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Ken } } I seem to have spent most of my life playing (!) with electron guns. How } often I have moved filaments forward or backwards, sharpened tungsten } filaments to obtain LaB6 type performance, lifted up anodes and checked out } different cathode shapes! } } To most people in laboratories a gun is what the manufacturer gives you, but } with the good old tungsten hairpin that may be considered to be just a } starting point. Total cathode geometry and the changes that you are able to } make regarding filament life in one direction and performance in the other, } are outside of the norm. Add to this raising the anode to improve } performance and few appreciate how you may transform an instrument. } } On tungsten filament temperature I have a great deal to say but as usual } from me it is probably controversial? I rarely fully saturate the gun! I } am a great believer in under saturating. I see no point in throwing away } filament life and with modern stabilities there is usually no point in doing } so. An amazing amount of work may be accomplished and for most SEM analysis } there is no point, it hardly can be said to improve x-ray resolution! } } I only saturate when working at the absolute limit at a particular kV and I } challenge anyone to see a visual degradation in any images I record through } using this technique; but only up to 100,000X. The big bonus for most labs } is this technique really does improve tungsten or even LaB6 filament life. } } Thus I agree with you with low filament currents it is oxidation that is the } final tungsten filament killer not evaporation! Too hot a filament and it } just evaporates but be more thoughtful and life is extended. We improve the } vacuum and the life is extended even further; but probably at the cost ($) } of an ion pump! } } Regards } } Steve } } Steve Chapman FRMS } Senior Consultant Protrain } For consultancy and training in electron microscopy world wide } Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 } www.emcourses.com } } } } } ---Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } Sent: 22 June 2011 18:35 } To: protrain-at-emcourses.com } Subject: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Steve, } The air, the air... oh, you mean all the hot air? You might have a point } ;-) } } The 11 uA emission current leads me to think that the lower temperature } enhances the effects of good vacuum. As for a more normal system, I've } found Amrays follow the manual, 40 hrs, ion pump or not, unless the vacuum } is especially poor. JEOLs seem about the same except their typical life is } 100-200 hours. I did have one dry pumped ETEC that would run for weeks and } months 24-7 in the 10e-8T range, but it was run slightly undersaturated } because life was more important than stability for the particular } experiment. } } Maybe you can shed some light on this thought. I've always wondered how } much effect the physical gun design has on the temperature required for a } particular emission current. Your example seems to indicate the same thing } that TEMs seem to indicate and that is that the lower emission currents, and } consequent lower temperatures result in oxidation being a larger factor than } evaporation on filament life, so better vacuum has a greater effect on lower } temperature filaments. Any thoughts? } } Ken } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] } Sent: Wednesday, June 22, 2011 12:52 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Ken } } I usually agree with your comments but this time I have to throw in a curved } ball. } } I often work with automated analysis SEMs which have a tungsten filament } life of upwards from 2500 hours; admittedly we run at 11 micro amps emission } with the filament well back. The instrument are not used by electron } microscopists so the idea behind this long life being that the service } technician is able to fit a new filament on their six monthly service } visits. } } We worked hard to obtain this life and one of the features that we found } that was very important in our development was the vacuum level. Thus we } run with an ion pump on the gun and the difference in filament life is } considerable. This is no criticism of the basic instrument, which exhibits a } gun vacuum level in its standard form the same if not better than its } competitors. } } Another area that the gun vacuum level influenced was the high voltage } stability. The instruments need to run for up to 24 hours unattended and it } is most important for the high voltage to remain absolutely stable over that } entire period of time. Double plots of high voltage level and gun vacuum } show that once the gun vacuum settles so does the high voltage. Thus the } operators wait for this stability period prior to starting a sample run; } proven to be the best practice. } } As a second point I know LaB6 users who have been forced to use tungsten } whilst waiting for a new source and they have also commented on the } increased filament life. } } Maybe I should say that not one of these comments relates to clients in the } United States; must be related to the type of air :-) } } Regards } } Steve } } Steve Chapman FRMS } Senior Consultant Protrain } For consultancy and training in electron microscopy world wide } Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 } www.emcourses.com } } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } } Sent: 22 June 2011 16:07 } To: protrain-at-emcourses.com } Subject: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Roy, } I beg to differ. I have not seen any significant increase in tungsten } filament life in an ion-pumped gun versus a normal gun in good condition in } an SEM. Poor vacuum will definitely shorten a W filament's life, but a } better vacuum makes very little difference due to the fact that W filaments } run hotter than LaB6 and evaporate faster. The reason poor vacuum } accelerates their demise is due to oxidation on top of evaporation. } } No W filament I've ever seen has lasted more than about 300 hours at } operating temperature in an SEM, 10E-7T vacuum notwithstanding. My } observations may not hold for a TEM because TEMs run at lower } temperatures/emission currents and therefore evaporation rates should be } significantly lower. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] } Sent: Wednesday, June 22, 2011 10:31 AM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John. } } Like Nicolas said here below, it's all a matter of vacuum. } Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs } usually have a filament life of way above 1000 hours. If you take the same } SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get } way above 1000 hours with the tungsten filament. } } So, in theory if your SEM gun has a very good vacuum, you should be getting } a very high lifetime from each tungsten filament. If not, you could expect } to get good result with the LaB6 filemant, but keep the lifetime of your } current tungsten one. Keep in mind that the LaB6 filament is exponentially } more expensive than tungsten. } } It'd be best to consult with Jeol in any case before you decide to do this. } } Regards, } Roy } } -----Original Message----- } X-from: Nicolas.Stephant-at-univ-nantes.fr } [mailto:Nicolas.Stephant-at-univ-nantes.fr] } Sent: Thursday, June 16, 2011 1:45 PM } To: Roy Golombick } Subject: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John } } LaB6 filament have to work in a good vacuum. There is some instruments } like your TEM working with LaB6 in a gun pumped by DP or turbo, but most } of the LaB6 TEM work with high vacuum in the gun provided by SIP. If } your vacuum system is very good you may use LaB6 but you must understand } that you will work on an edge. Anyway, you may try without danger for } your instrument and you will see if LaB6 stay save during one year } (good) or one month. Please note that you may use another wehnelt cap } for this kind of filament and you have to change distance beetween } filament tip and wehnelt cap. First eating of this filament must be done } very slowy (about one hour) after almost one night of pumping. Working } with LaB6 is very comfortable because the brigtness is very high and it } may increase significantly top end result. } Regards } } Nicolas STEPHANT } } Université de Nantes } Institut Jean Rouxel } Service de microscopie électronique à balayage et microanalyse } 2 rue de la Houssinière } BP 92208 } 44322 Nantes cédex 3 } } Tél : 02 40 37 64 26 } Mél : nicolas.stephant-at-univ-nantes.fr } Web : http://www.univ-nantes.fr/smebm } } "Le monde n'existe que pour autant que nous sommes capables d'en } produire une image" } } C.G Jung } john.mitchels-at-gmail.com a écrit : } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hi Listers } } } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } } enough in which to use a LaB6. The engineers seem to have mix } } opinions, as does the great google. I would like comments from anyone } } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } } The microscope is behaving itself very well, no leaks everything is as } } good as it gets given its age. With one fitted do you notice any } } significant difference when working at the top end? } } } } Thanks in advance. } } } } John } } } } ==============================Original } Headers============================== } } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com } [209.85.210.41]) } } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5G8RUwL002495 } } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 } -0500 } } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 } 01:27:29 -0700 (PDT) } } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 4, 30 -- d=gmail.com; s=gamma; } } 4, 30 -- } h=domainkey-signature:mime-version:date:message-id:subject:from:to } } 4, 30 -- :content-type; } } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } } 4, 30 -- } b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } } 4, 30 -- } fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 4, 30 -- d=gmail.com; s=gamma; } } 4, 30 -- } h=mime-version:date:message-id:subject:from:to:content-type; } } 4, 30 -- } b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } } 4, 30 -- } fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } } 4, 30 -- MIME-Version: 1.0 } } 4, 30 -- Received: by 10.68.51.201 with SMTP id } m9mr351917pbo.135.1308212849644; Thu, } } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 } -0700 (PDT) } } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } } 4, 30 -- To: Microscopy-at-microscopy.com } } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } } ==============================End of - } Headers============================== } } } } -- } } } ==============================Original Headers============================== } 8, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 16 05:37:15 2011 } 8, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5GAbEpl020489 } 8, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 05:37:14 } -0500 } 8, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr } [194.214.57.230]) } 8, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id } p5GAb9nv007050; } 8, 25 -- Thu, 16 Jun 2011 12:37:10 +0200 } 8, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) } 8, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id } p5GAb7iQ000928 } 8, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 } verify=NO); } 8, 25 -- Thu, 16 Jun 2011 12:37:07 +0200 } 8, 25 -- Message-ID: {4DF9DF3D.3020203-at-univ-nantes.fr} } 8, 25 -- Date: Thu, 16 Jun 2011 12:47:25 +0200 } 8, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} } 8, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr } 8, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: john.mitchels-at-gmail.com, Microscopy-at-microscopy.com } 8, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } 8, 25 -- References: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} } 8, 25 -- In-Reply-To: {201106160835.p5G8ZN5o017617-at-ns.microscopy.com} } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-Miltered: at b-mail with ID 4DF9DCD5.000 by Joe's j-chkmail (http } : // j-chkmail dot ensmp dot fr)! } 8, 25 -- X-j-chkmail-Enveloppe: } 4DF9DCD5.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Ste } phant-at-univ-nantes.fr} } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 21, 25 -- From Roy-at-picotech.co.il Wed Jun 22 09:29:42 2011 } 21, 25 -- Received: from mail.picotech.co.il (mail.picotech.co.il } [212.143.255.226]) } 21, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5METfrh003527 } 21, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 09:29:41 } -0500 } 21, 25 -- Received: from SERVER01.picotech.local ([192.168.1.2]) by server01 } 21, 25 -- ([192.168.1.2]) with mapi; Wed, 22 Jun 2011 17:27:59 +0300 } 21, 25 -- From: Roy Golombick {Roy-at-picotech.co.il} } 21, 25 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 21, 25 -- Disposition-Notification-To: Roy Golombick {Roy-at-picotech.co.il} } 21, 25 -- Date: Wed, 22 Jun 2011 17:27:59 +0300 } 21, 25 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it } possible? } 21, 25 -- Thread-Topic: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it } possible? } 21, 25 -- Thread-Index: AcwsEjA2FcR8LNxjSzquPhESHEjIbQE1a7nA } 21, 25 -- Message-ID: {ABB156E109DC9A42B8109368CA6F4895178F899703-at-server01} } 21, 25 -- References: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} } 21, 25 -- In-Reply-To: {201106161045.p5GAjCgF032488-at-ns.microscopy.com} } 21, 25 -- Accept-Language: en-US } 21, 25 -- Content-Language: en-US } 21, 25 -- X-MS-Has-Attach: } 21, 25 -- X-MS-TNEF-Correlator: } 21, 25 -- acceptlanguage: en-US } 21, 25 -- Content-Type: text/plain; charset="iso-8859-1" } 21, 25 -- MIME-Version: 1.0 } 21, 25 -- Content-Transfer-Encoding: 8bit } 21, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p5METfrh003527 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 33, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 10:06:24 2011 } 33, 28 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.120]) } 33, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5MF6NHc030811 } 33, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 10:06:24 } -0500 } 33, 28 -- X-Authority-Analysis: v=1.1 } cv=pLPlkKcK38cN4Cv4YkVeTGGRYpmKez/IrX640LVKWnc= c=1 sm=0 a=nGPnte84VBEA:10 } a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 } a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 a=tkoM7y6riZj4z8YaxSEA:9 } a=ZHt2qSYvVdxlc-6TZLkA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 } a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 } a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 33, 28 -- X-Cloudmark-Score: 0 } 33, 28 -- X-Originating-IP: 72.224.149.57 } 33, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3813] helo=Ken) } 33, 28 -- by cdptpa-oedge04.mail.rr.com (envelope-from } {kenconverse-at-qualityimages.biz} ) } 33, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP } 33, 28 -- id D4/E5-20202-FE4020E4; Wed, 22 Jun 2011 15:06:23 +0000 } 33, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 33, 28 -- To: {Roy-at-picotech.co.il} , "MSA Listserver" } {microscopy-at-microscopy.com} } 33, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } 33, 28 -- Date: Wed, 22 Jun 2011 11:06:10 -0400 } 33, 28 -- Message-ID: {99C306969A954EAE952E39FD81828717-at-Ken} } 33, 28 -- MIME-Version: 1.0 } 33, 28 -- Content-Type: text/plain; } 33, 28 -- charset="iso-8859-1" } 33, 28 -- X-Priority: 3 (Normal) } 33, 28 -- X-MSMail-Priority: Normal } 33, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 33, 28 -- In-Reply-To: {201106221431.p5MEVDhm008546-at-ns.microscopy.com} } 33, 28 -- Thread-Index: Acww6Qn916P2m7amSB6IsRlktAomswAAqauA } 33, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 } 33, 28 -- Importance: Normal } 33, 28 -- Content-Transfer-Encoding: 8bit } 33, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p5MF6NHc030811 } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 52, 28 -- From protrain-at-emcourses.com Wed Jun 22 11:50:32 2011 } 52, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net } [196.26.208.170]) } 52, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5MGoU41032318 } 52, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 11:50:31 } -0500 } 52, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:51659 } helo=ProtrainPC) } 52, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) } 52, 28 -- (envelope-from {protrain-at-emcourses.com} ) } 52, 28 -- id 1QZQdG-000Loz-0E by authid } {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 22 Jun 2011 } 18:50:27 +0200 } 52, 28 -- Reply-To: {protrain-at-emcourses.com} } 52, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} } 52, 28 -- To: {kenconverse-at-qualityimages.biz} } 52, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} } 52, 28 -- References: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} } 52, 28 -- In-Reply-To: {201106221507.p5MF7Tbo032023-at-ns.microscopy.com} } 52, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it } possible? } 52, 28 -- Date: Wed, 22 Jun 2011 17:49:52 +0100 } 52, 28 -- Organization: Protrain } 52, 28 -- Message-ID: {004d01cc30fc$7b2a66d0$717f3470$-at-com} } 52, 28 -- MIME-Version: 1.0 } 52, 28 -- Content-Type: text/plain; } 52, 28 -- charset="iso-8859-1" } 52, 28 -- X-Mailer: Microsoft Office Outlook 12.0 } 52, 28 -- Thread-Index: Acww7ieAAtwiidP2TCyhjyuu2/R/AQABzfdA } 52, 28 -- Content-Language: en-gb } 52, 28 -- X-Scan-Signature: c7f1ef588874116c1f795ee3e363243c{443}} } 52, 28 -- X-Trace: smtp01.dial-up.net 1QZQdG-000Loz-0E } 53857f849a2877cf96ab3a0e288b1a64 } 52, 28 -- Content-Transfer-Encoding: 8bit } 52, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p5MGoU41032318 } ==============================End of - Headers============================== } } } } } ==============================Original Headers============================== } 66, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 12:33:48 2011 } 66, 28 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.120]) } 66, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5MHXmOw018481 } 66, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 12:33:48 } -0500 } 66, 28 -- X-Authority-Analysis: v=1.1 } cv=aeMH4JcVOnVr0LmJAzqEvfnmJyuaZufWdlng4HTRGCk= c=1 sm=0 a=nGPnte84VBEA:10 } a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=TjS3GnXOAAAA:8 } a=Zx37jsudAAAA:8 a=oJzxCKBaAAAA:8 a=pGLkceISAAAA:8 a=1XWaLZrsAAAA:8 } a=ayC55rCoAAAA:8 a=h_vVgvW_AAAA:8 a=E0F6PzzqAAAA:8 a=5qZVn9eiuOZf3ur7tZcA:9 } a=pz22PO0dAz2PExYwF1kA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 } a=Gn2-7-Lc7R4A:10 a=n0CbYuQnzTYA:10 a=a7qs6diK2AgA:10 a=RyjLG6jaqdoA:10 } a=4NIop_tvI_gA:10 a=3_2eAsHTqJoA:10 a=18pRNVDt_m0A:10 a=MSl-tDqOz04A:10 } a=G91thX30KR8A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 66, 28 -- X-Cloudmark-Score: 0 } 66, 28 -- X-Originating-IP: 72.224.149.57 } 66, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1109] helo=Ken) } 66, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from } {kenconverse-at-qualityimages.biz} ) } 66, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP } 66, 28 -- id F9/A2-03893-B77220E4; Wed, 22 Jun 2011 17:33:48 +0000 } 66, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 66, 28 -- To: {protrain-at-emcourses.com} , "MSA Listserver" } {microscopy-at-microscopy.com} } 66, 28 -- Subject: RE: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } 66, 28 -- Date: Wed, 22 Jun 2011 13:33:34 -0400 } 66, 28 -- Message-ID: {645DAD6306BA4DD9AD55BD210C83BA7A-at-Ken} } 66, 28 -- MIME-Version: 1.0 } 66, 28 -- Content-Type: text/plain; } 66, 28 -- charset="iso-8859-1" } 66, 28 -- X-Priority: 3 (Normal) } 66, 28 -- X-MSMail-Priority: Normal } 66, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 66, 28 -- In-Reply-To: {201106221652.p5MGqBWY003344-at-ns.microscopy.com} } 66, 28 -- Thread-Index: Acww/LtZV9um7gK0RnWik8TGADTktQAA2pnQ } 66, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 } 66, 28 -- Importance: Normal } 66, 28 -- Content-Transfer-Encoding: 8bit } 66, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p5MHXmOw018481 } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 85, 28 -- From protrain-at-emcourses.com Wed Jun 22 14:31:29 2011 } 85, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) } 85, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MJVRiF010165 } 85, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 14:31:27 -0500 } 85, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:53191 helo=ProtrainPC) } 85, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) } 85, 28 -- (envelope-from {protrain-at-emcourses.com} ) } 85, 28 -- id 1QZT91-00009r-QT by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Wed, 22 Jun 2011 21:31:25 +0200 } 85, 28 -- Reply-To: {protrain-at-emcourses.com} } 85, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} } 85, 28 -- To: {kenconverse-at-qualityimages.biz} } 85, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} } 85, 28 -- References: {201106221734.p5MHYhsW019899-at-ns.microscopy.com} } 85, 28 -- In-Reply-To: {201106221734.p5MHYhsW019899-at-ns.microscopy.com} } 85, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? } 85, 28 -- Date: Wed, 22 Jun 2011 20:31:18 +0100 } 85, 28 -- Organization: Protrain } 85, 28 -- Message-ID: {000601cc3112$f7af60a0$e70e21e0$-at-com} } 85, 28 -- MIME-Version: 1.0 } 85, 28 -- Content-Type: text/plain; } 85, 28 -- charset="iso-8859-1" } 85, 28 -- X-Mailer: Microsoft Office Outlook 12.0 } 85, 28 -- Thread-Index: AcwxArmSvc7TTHkSRlOQmywslhoTnAADB+mQ } 85, 28 -- Content-Language: en-gb } 85, 28 -- X-Scan-Signature: 60128edfd7223a9476a9143ebe930403{696}} } 85, 28 -- X-Trace: smtp01.dial-up.net 1QZT91-00009r-QT 48489186918435a5fb73c649123431f3 } 85, 28 -- Content-Transfer-Encoding: 8bit } 85, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MJVRiF010165 } ==============================End of - Headers============================== } }
-- John Mansfield PhD Cphys MInstP 2010 Microscopy & Microanalysis Program Chair North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 11, 20 -- From jfmjfm-at-umich.edu Wed Jun 22 15:28:53 2011 11, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 11, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MKSrJl011881 11, 20 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 15:28:53 -0500 11, 20 -- Received: FROM 52.129.241.10.in-addr.arpa (m2d2836d0.tmodns.net [208.54.40.45]) 11, 20 -- By hellskitchen.mr.itd.umich.edu ID 4E025080.E4E96.15640 ; 11, 20 -- Authuser jfmjfm; 11, 20 -- 22 Jun 2011 16:28:49 EDT 11, 20 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 11, 20 -- Mime-Version: 1.0 (Apple Message framework v1082.1) 11, 20 -- Content-Type: text/plain; charset=iso-8859-1 11, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 11, 20 -- In-Reply-To: {201106221942.p5MJgD5j031767-at-ns.microscopy.com} 11, 20 -- Date: Wed, 22 Jun 2011 16:28:48 -0400 11, 20 -- Message-Id: {B0E9D25C-2528-4635-AE27-5B2345DE1737-at-umich.edu} 11, 20 -- References: {201106221942.p5MJgD5j031767-at-ns.microscopy.com} 11, 20 -- To: microscopy-at-microscopy.com 11, 20 -- X-Mailer: Apple Mail (2.1082.1) 11, 20 -- Content-Transfer-Encoding: 8bit 11, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MKSrJl011881 ==============================End of - Headers==============================
Micro/macro-gigapan for the image-tiling and stepping: http://www.gigamacro.com/gigapixel_macro_photography_technology.php
http://smallworld.gigapan.org/
Alternatively, since the wafer is "flat" you could invert an EpsonV750 and move the wafer to within 1mm of the scanner's surface. 2400dpi (true) is just about 10micron/pixel. However, the image will be 600MBytes. I do this for kind of "macro" imaging all the time.
regards,
JQuinn
PS: "silicon wafer", not "silicone wafer".
At 12:33 PM 6/22/2011, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 14, 16 -- From jquinn-at-ms.cc.sunysb.edu Wed Jun 22 16:38:23 2011 14, 16 -- Received: from mailrelay.stonybrook.edu (mrs.stonybrook.edu [129.49.1.206]) 14, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MLcNba029292 14, 16 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 16:38:23 -0500 14, 16 -- Received: from happy-b95c68896.ms.cc.sunysb.edu (ceas2064.matscieng.sunysb.edu [129.49.36.224]) 14, 16 -- by mailrelay.stonybrook.edu (8.14.2/8.14.2) with ESMTP id p5MLcImO021805 14, 16 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:38:22 -0400 (EDT) 14, 16 -- Message-Id: {201106222138.p5MLcImO021805-at-mailrelay.stonybrook.edu} 14, 16 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 14, 16 -- Date: Wed, 22 Jun 2011 17:38:08 -0400 14, 16 -- To: microscopy-at-microscopy.com 14, 16 -- From: Jim Quinn {jquinn-at-ms.cc.sunysb.edu} 14, 16 -- Subject: Re: [Microscopy] RE: Suggestions for a 3-D Imager 14, 16 -- Mime-Version: 1.0 14, 16 -- Content-Type: text/plain; charset="us-ascii" 14, 16 -- X-Brightmail-Tracker: AAAAAQAAAUA= ==============================End of - Headers==============================
We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the spectra. I understood that these elements are from the polepiece and other components in the sample area.
When a student submitted a manuscript with these elements in the spectrum, along with the other elements of interest (IN, Sb), a reviewer said that the TEM instruments are strictly restricted from using the magnetic impurities/parts, so there must be other sources for iron.
Comments? How would you respond to this reviewer.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 11, 28 -- From dsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 11, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MMDw5g013430 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 11, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB01F.purdue.lcl 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 11, 28 -- Subject: Tem EDX 11, 28 -- Thread-Topic: Tem EDX 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-PMX-Version: 5.5.9.388399 11, 28 -- X-PerlMx-Virus-Scanned: Yes 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MMDw5g013430 ==============================End of - Headers==============================
The pole-pieces are necessarily a magnetic alloy to "focus" the magnetic flux lines. The usual composition is Permalloy or a (Co, Fe) alloy. The Cu is likely from the TEM grid. You can even check the Wikipedia entry on "electron microscopy" which talks about pole pieces being made of magnetic material. Sounds like the reviewer needs his head smacked! (in my not so humble opinion!)
from Williams & Carter section 6.3
{...snip...} To make a magnetic electron lens we need two parts. Both are drawn schematically in cross section in Figure 6.6. First there is a cylindrically symmetrical core of soft magnetic material such as soft iron, with a hole drilled through it. We call this soft iron a polepiece and the hole is called the bore of the polepiece. (Soft refers to the magnetic not the mechanical behavior.) {...snip...}
Cheers, Henk
At 6/22/2011 6:14 PM, dsherman-at-purdue.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the } spectra. I understood that these elements are from the polepiece and other } components in the sample area. } } When a student submitted a manuscript with these elements in the spectrum, } along with the other elements of interest (IN, Sb), a reviewer said that } the TEM instruments are strictly restricted from using the magnetic } impurities/parts, so there must be other sources for iron. } } Comments? How would you respond to this reviewer. } } Debby } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy } } } } } } } ==============================Original Headers============================== } 11, 28 -- From dsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 } 11, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) } 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MMDw5g013430 } 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 } 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) } 11, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 } 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 } 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB01F.purdue.lcl } 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 } 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 } 11, 28 -- Subject: Tem EDX } 11, 28 -- Thread-Topic: Tem EDX } 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== } 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} } 11, 28 -- Accept-Language: en-US } 11, 28 -- Content-Language: en-US } 11, 28 -- X-MS-Has-Attach: } 11, 28 -- X-MS-TNEF-Correlator: } 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 11, 28 -- acceptlanguage: en-US } 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" } 11, 28 -- MIME-Version: 1.0 } 11, 28 -- X-PMX-Version: 5.5.9.388399 } 11, 28 -- X-PerlMx-Virus-Scanned: Yes } 11, 28 -- Content-Transfer-Encoding: 8bit } 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MMDw5g013430 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 9, 27 -- From colijn.1-at-osu.edu Wed Jun 22 17:51:26 2011 9, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MMpQQa029905 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:51:26 -0500 9, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 27 -- id {01O2SLFY9AMO9FF1BL-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 9, 27 -- Wed, 22 Jun 2011 18:51:25 -0400 (EDT) 9, 27 -- Received: from [192.168.1.102] 9, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 9, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 27 -- with ESMTPA id {01O2SLFNKXN49FAJ9C-at-ecr6.ohio-state.edu} ; Wed, 9, 27 -- 22 Jun 2011 18:51:25 -0400 (EDT) 9, 27 -- Date: Wed, 22 Jun 2011 18:51:08 -0400 9, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 27 -- Subject: Re: [Microscopy] Tem EDX 9, 27 -- In-reply-to: {201106222214.p5MMEjdj015302-at-ns.microscopy.com} 9, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 27 -- To: dsherman-at-purdue.edu, microscopy-at-microscopy.com 9, 27 -- Message-id: {4E0271DC.5080801-at-osu.edu} 9, 27 -- MIME-version: 1.0 9, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 27 -- Content-transfer-encoding: 7bit 9, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) 9, 27 -- Gecko/20110414 Thunderbird/3.1.10 9, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 27 -- References: {201106222214.p5MMEjdj015302-at-ns.microscopy.com} ==============================End of - Headers==============================
I think what he or she is saying is that the instruments don't have iron or other magnetic materials close to the beam to avoid magnetic distortions, so there shouldn't be any Iron detected. But aren't the pole pieces made out of magnetic materials which could include iron? You could simply show that you get an Fe peak when you put in just the holder, or perhaps the holder and an empty grid. I don't think you would have to show that in the publication itself.
mike --- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, June 22, 2011 4:20 PM To: mike.bode-at-resaltatech.com
Hi all,
We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the spectra. I understood that these elements are from the polepiece and other components in the sample area.
When a student submitted a manuscript with these elements in the spectrum, along with the other elements of interest (IN, Sb), a reviewer said that the TEM instruments are strictly restricted from using the magnetic impurities/parts, so there must be other sources for iron.
Comments? How would you respond to this reviewer.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 11, 28 -- From dsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 11, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MMDw5g013430 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 11, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB01F.purdue.lcl 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 11, 28 -- Subject: Tem EDX 11, 28 -- Thread-Topic: Tem EDX 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-PMX-Version: 5.5.9.388399 11, 28 -- X-PerlMx-Virus-Scanned: Yes 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MMDw5g013430 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 32 -- From mike.bode-at-resaltatech.com Wed Jun 22 18:07:26 2011 28, 32 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 28, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MN7QLe013795 28, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:07:26 -0500 28, 32 -- Received: from c-174-51-0-206.hsd1.co.comcast.net ([174.51.0.206]:17099 helo=resdtpden01) 28, 32 -- by web38.justhost.com with esmtpa (Exim 4.69) 28, 32 -- (envelope-from {mike.bode-at-resaltatech.com} ) 28, 32 -- id 1QZWWC-0004KW-Hr; Wed, 22 Jun 2011 18:07:32 -0500 28, 32 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 28, 32 -- To: {Microscopy-at-microscopy.com} 28, 32 -- Cc: {dsherman-at-purdue.edu} 28, 32 -- References: {201106222220.p5MMK8Wr026883-at-ns.microscopy.com} 28, 32 -- In-Reply-To: {201106222220.p5MMK8Wr026883-at-ns.microscopy.com} 28, 32 -- Subject: RE: [Microscopy] Tem EDX 28, 32 -- Date: Wed, 22 Jun 2011 17:07:20 -0600 28, 32 -- Message-ID: {009c01cc3131$253858b0$6fa90a10$-at-bode-at-resaltatech.com} 28, 32 -- MIME-Version: 1.0 28, 32 -- Content-Type: text/plain; 28, 32 -- charset="iso-8859-1" 28, 32 -- X-Mailer: Microsoft Office Outlook 12.0 28, 32 -- Content-Language: en-us 28, 32 -- Thread-Index: AcwxKo68Cwvr+ROXRJC8a5omtFtEcQABHgsg 28, 32 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 28, 32 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 28, 32 -- X-AntiAbuse: Original Domain - microscopy.com 28, 32 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 28, 32 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 28, 32 -- X-Source: 28, 32 -- X-Source-Args: 28, 32 -- X-Source-Dir: 28, 32 -- Content-Transfer-Encoding: 8bit 28, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MN7QLe013795 ==============================End of - Headers==============================
Debbie, I'm with Henk. The reviewer needs a smack. Very few EMs use anything other than electromagnetic lenses made of iron (often a 1001 or 1002 very mild steel to reduce hysterisis). The lens is going to be very close to the sample. Often SEM lenses are plated with Rh and if the EDX detector is pulled too far out, you get Rh in your spectrum. I'm not sure if you have any motion capability with the TEM. Is the collimator properly mounted? That can often make a big difference in what gets picked up from the microscope itself.
The reviewer's reasoning is wrong. Period.
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: mike.bode-at-resaltatech.com [mailto:mike.bode-at-resaltatech.com] Sent: Wednesday, June 22, 2011 7:09 PM To: kenconverse-at-qualityimages.biz
Hi Debby,
I think what he or she is saying is that the instruments don't have iron or other magnetic materials close to the beam to avoid magnetic distortions, so there shouldn't be any Iron detected. But aren't the pole pieces made out of magnetic materials which could include iron? You could simply show that you get an Fe peak when you put in just the holder, or perhaps the holder and an empty grid. I don't think you would have to show that in the publication itself.
mike --- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, June 22, 2011 4:20 PM To: mike.bode-at-resaltatech.com
Hi all,
We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the spectra. I understood that these elements are from the polepiece and other components in the sample area.
When a student submitted a manuscript with these elements in the spectrum, along with the other elements of interest (IN, Sb), a reviewer said that the TEM instruments are strictly restricted from using the magnetic impurities/parts, so there must be other sources for iron.
Comments? How would you respond to this reviewer.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 11, 28 -- From dsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 11, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MMDw5g013430 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 11, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB01F.purdue.lcl 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 11, 28 -- Subject: Tem EDX 11, 28 -- Thread-Topic: Tem EDX 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-PMX-Version: 5.5.9.388399 11, 28 -- X-PerlMx-Virus-Scanned: Yes 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MMDw5g013430 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 32 -- From mike.bode-at-resaltatech.com Wed Jun 22 18:07:26 2011 28, 32 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 28, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MN7QLe013795 28, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:07:26 -0500 28, 32 -- Received: from c-174-51-0-206.hsd1.co.comcast.net ([174.51.0.206]:17099 helo=resdtpden01) 28, 32 -- by web38.justhost.com with esmtpa (Exim 4.69) 28, 32 -- (envelope-from {mike.bode-at-resaltatech.com} ) 28, 32 -- id 1QZWWC-0004KW-Hr; Wed, 22 Jun 2011 18:07:32 -0500 28, 32 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 28, 32 -- To: {Microscopy-at-microscopy.com} 28, 32 -- Cc: {dsherman-at-purdue.edu} 28, 32 -- References: {201106222220.p5MMK8Wr026883-at-ns.microscopy.com} 28, 32 -- In-Reply-To: {201106222220.p5MMK8Wr026883-at-ns.microscopy.com} 28, 32 -- Subject: RE: [Microscopy] Tem EDX 28, 32 -- Date: Wed, 22 Jun 2011 17:07:20 -0600 28, 32 -- Message-ID: {009c01cc3131$253858b0$6fa90a10$-at-bode-at-resaltatech.com} 28, 32 -- MIME-Version: 1.0 28, 32 -- Content-Type: text/plain; 28, 32 -- charset="iso-8859-1" 28, 32 -- X-Mailer: Microsoft Office Outlook 12.0 28, 32 -- Content-Language: en-us 28, 32 -- Thread-Index: AcwxKo68Cwvr+ROXRJC8a5omtFtEcQABHgsg 28, 32 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 28, 32 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 28, 32 -- X-AntiAbuse: Original Domain - microscopy.com 28, 32 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 28, 32 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 28, 32 -- X-Source: 28, 32 -- X-Source-Args: 28, 32 -- X-Source-Dir: 28, 32 -- Content-Transfer-Encoding: 8bit 28, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MN7QLe013795 ==============================End of - Headers==============================
==============================Original Headers============================== 39, 28 -- From kenconverse-at-qualityimages.biz Wed Jun 22 18:27:12 2011 39, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.123]) 39, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MNRC6R030051 39, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:27:12 -0500 39, 28 -- X-Authority-Analysis: v=1.1 cv=8mDY8c80ZOa76EOwICuS+E2YRQjxDgO9xqUnRMONc7w= c=1 sm=0 a=JAfuBhRnIwsA:10 a=8nJEP1OIZ-IA:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=CmOMp8_9AAAA:8 a=Zx37jsudAAAA:8 a=35Thz0BbAAAA:8 a=C_IRinGWAAAA:8 a=BY8XqHikAAAA:8 a=1UmCCR50HzkwtIKoOOEA:9 a=J5zzDqagygR_5KabAsoA:7 a=wPNLvfGTeEIA:10 a=5JUlSvKOtR0A:10 a=GLDqNL25GNkA:10 a=nJqo5kjLZsgA:10 a=AtnM69iVAwYA:10 a=-DdyyhJ99MMA:10 a=ZiPVLjR5StsA:10 a=RyjLG6jaqdoA:10 a=ayl80Gp6apUA:10 a=3_2eAsHTqJoA:10 a=PV6Khv4P5wQA:10 a=BQPYhLvFQawA:10 a=SYU6H1v74FsA:10 a=G91thX30KR8A:10 a=p44A_C2j4aAslhd9:21 a=S1d8bHey0baPm0-A:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 39, 28 -- X-Cloudmark-Score: 0 39, 28 -- X-Originating-IP: 72.224.149.57 39, 28 -- Received: from [72.224.149.57] ([72.224.149.57:3054] helo=Ken) 39, 28 -- by cdptpa-oedge03.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 39, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 39, 28 -- id F7/15-00666-F4A720E4; Wed, 22 Jun 2011 23:27:12 +0000 39, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 39, 28 -- To: {mike.bode-at-resaltatech.com} , "MSA Listserver" {microscopy-at-microscopy.com} 39, 28 -- Subject: RE: [Microscopy] RE: Tem EDX 39, 28 -- Date: Wed, 22 Jun 2011 19:26:58 -0400 39, 28 -- Message-ID: {11A9F2FDC66A4967B657F5523044613C-at-Ken} 39, 28 -- MIME-Version: 1.0 39, 28 -- Content-Type: text/plain; 39, 28 -- charset="iso-8859-1" 39, 28 -- X-Priority: 3 (Normal) 39, 28 -- X-MSMail-Priority: Normal 39, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 39, 28 -- In-Reply-To: {201106222308.p5MN8iXJ017223-at-ns.microscopy.com} 39, 28 -- Thread-Index: AcwxMVXTvyzKKqKXQzasxdPJDOKonwAAWnmQ 39, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 39, 28 -- Importance: Normal 39, 28 -- Content-Transfer-Encoding: 8bit 39, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MNRC6R030051 ==============================End of - Headers==============================
we're running our tungsten source SEM (no IGP, whole instrument vented and TP off during sample exchange...) in two modes:
Automated mineralogy, EDS mapping...: 40uA emission, fila tip way back in Wehnelt, not undersaturated as that seems to help beam stability, wait 1 hour for stabilisation after pump down and HV on. Filament heat setting at around 1.5-1.6V (not sure what temperature that corresponds to) after initial burn in. Filament lifetimes are between 700h and over 2000h, but usually over 1000h. Penning gauge usually reads mid E-4 Pa values during runs, ultimate pressure of the system around 5E-5 Pa.
Other SEM operation (biol. imaging, CL...): 100uA emission, fila tip much closer to grid cap, slightly undersaturated. Fila usually breaks at around 2 V after 40-150h, usually somewhere around 100h.
We have a second Wehnelt prepared in a desiccator for the other mode so we swap them, usually every 1-2 weeks. Fila lifetimes appear to be longer if one Wehnelt is in the SEM all the time, i.e. not swapped and stored externally.
Does this add some useful "data" to the oxidation / vac. quality vs. evaporation considerations? Cheers,
Karsten
-- Dr Karsten Goemann Research Fellow, Electron Microscopy & X-ray Microanalysis Central Science Laboratory, University of Tasmania Private Bag 74, Hobart TAS 7001, Australia Tel.: +61 (0) 3 6226 2146 (office & labs) Mobile: +61 (0) 407 101 990 Fax: +61 (0) 3 6226 2494 (general CSL) E-mail: Karsten.Goemann-at-utas.edu.au http://www.utas.edu.au/csl
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 23 -- From Karsten.Goemann-at-utas.edu.au Wed Jun 22 18:49:24 2011 13, 23 -- Received: from mx2.utas.edu.au (ironport.its.utas.edu.au [131.217.6.11]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MNnLh2013964 13, 23 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:49:22 -0500 13, 23 -- X-IronPort-AV: E=Sophos;i="4.65,409,1304258400"; 13, 23 -- d="scan'208";a="24609081" 13, 23 -- Received: from unknown (HELO mmp-sby1.its.utas.edu.au) ([10.10.3.71]) 13, 23 -- by ironport-outgoing.utas.edu.au with ESMTP; 23 Jun 2011 09:49:15 +1000 13, 23 -- MIME-version: 1.0 13, 23 -- Content-type: text/plain; charset=iso-8859-1; format=flowed 13, 23 -- Received: from [131.217.50.60] by mmp-sby1.its.utas.edu.au 13, 23 -- (Sun Java(tm) System Messaging Server 7u3-17.01 64bit (built Jun 8 2010)) 13, 23 -- with ESMTPA id {0LN700MP9U626660-at-mmp-sby1.its.utas.edu.au} for 13, 23 -- microscopy-at-microscopy.com; Thu, 23 Jun 2011 09:49:15 +1000 (EST) 13, 23 -- Message-id: {a06240801ca28292a0f77-at-[131.217.50.60]} 13, 23 -- In-reply-to: {201106221738.p5MHci6c026839-at-ns.microscopy.com} 13, 23 -- References: {201106221738.p5MHci6c026839-at-ns.microscopy.com} 13, 23 -- Date: Thu, 23 Jun 2011 09:49:29 +1000 13, 23 -- To: microscopy-at-microscopy.com 13, 23 -- From: Karsten Goemann {Karsten.Goemann-at-utas.edu.au} 13, 23 -- Subject: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MNnLh2013964 ==============================End of - Headers==============================
Thanks to all for the quick response. Consensus is that the reviewer is incorrect as many scopes do see Fe and Co in EDX spectra from the polepiece. The amount may depend on the type of microscope, detector, etc but apparently many instruments show this.
I didn't mention this in my first E-mail but I regularly take a background spectrum and they invariably show Fe and Co in our instrument. It is difficult to do a background subtraction and remove all of it. Presumably this is because the sample does deflect many more electrons than just the support film resulting in more X-ray production from other areas within the objective lens.
We also have a cryo blade that is made of copper and also the connection from the external nitrogen dewar to the decontaminator is a copper braid. Thus, even when using a low background (Beryllium) holder and nickel grid we often get a small amount of Cu. This may not show up with a SiLi detector but we get so many counts with the SDD that this may translate into higher background peaks for some of these elements.
Debby
On 6/22/11 6:15 PM, "Debby Sherman" {dsherman-at-purdue.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi all, } } We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the } spectra. I understood that these elements are from the polepiece and other } components in the sample area. } } When a student submitted a manuscript with these elements in the spectrum, } along with the other elements of interest (IN, Sb), a reviewer said that } the TEM instruments are strictly restricted from using the magnetic } impurities/parts, so there must be other sources for iron. } } Comments? How would you respond to this reviewer. } } Debby } } --- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy } } } } } } } ==============================Original Headers============================== } 11, 28 -- From dsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 } 11, 28 -- Received: from mailhub128.itcs.purdue.edu } (mailhub128.itcs.purdue.edu [128.210.5.128]) } 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p5MMDw5g013430 } 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 } 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu } [172.21.6.90]) } 11, 28 -- by mailhub128.itcs.purdue.edu } (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 } 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 } 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by } WPPEXHUB01F.purdue.lcl } 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 } 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 } 11, 28 -- Subject: Tem EDX } 11, 28 -- Thread-Topic: Tem EDX } 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== } 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} } 11, 28 -- Accept-Language: en-US } 11, 28 -- Content-Language: en-US } 11, 28 -- X-MS-Has-Attach: } 11, 28 -- X-MS-TNEF-Correlator: } 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 11, 28 -- acceptlanguage: en-US } 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" } 11, 28 -- MIME-Version: 1.0 } 11, 28 -- X-PMX-Version: 5.5.9.388399 } 11, 28 -- X-PerlMx-Virus-Scanned: Yes } 11, 28 -- Content-Transfer-Encoding: 8bit } 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p5MMDw5g013430 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 29 -- From dsherman-at-purdue.edu Wed Jun 22 19:30:48 2011 9, 29 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5N0Um1s030596 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 19:30:48 -0500 9, 29 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 9, 29 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5N0UlfB014850 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 20:30:47 -0400 9, 29 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB04H.purdue.lcl 9, 29 -- ([::1]) with mapi; Wed, 22 Jun 2011 20:30:47 -0400 9, 29 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 9, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 9, 29 -- Date: Wed, 22 Jun 2011 20:30:43 -0400 9, 29 -- Subject: Re: [Microscopy] Tem EDX 9, 29 -- Thread-Topic: [Microscopy] Tem EDX 9, 29 -- Thread-Index: AcwxKc+ZrMVcwD1+REutdyv38FFgNQAEvmXQ 9, 29 -- Message-ID: {CA280173.F573%dsherman-at-purdue.edu} 9, 29 -- In-Reply-To: {201106222215.p5MMF71e016120-at-ns.microscopy.com} 9, 29 -- Accept-Language: en-US 9, 29 -- Content-Language: en-US 9, 29 -- X-MS-Has-Attach: 9, 29 -- X-MS-TNEF-Correlator: 9, 29 -- user-agent: Microsoft-Entourage/13.8.0.101117 9, 29 -- acceptlanguage: en-US 9, 29 -- Content-Type: text/plain; charset="iso-8859-1" 9, 29 -- MIME-Version: 1.0 9, 29 -- X-PMX-Version: 5.5.9.388399 9, 29 -- X-PerlMx-Virus-Scanned: Yes 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5N0Um1s030596 ==============================End of - Headers==============================
Some time ago I wrote about stability and the time taken to achieve heat gained in the high voltage generator to equal heat lost.
For a typical SEM from first switching on the high voltage through to stability takes about 45 minutes. With a TEM an oil filled tank at 100kV takes about 2 to 3 hours but a gas filled tank is usually less than an hour. If you are changing specimens within a short time period the time to stability should be reduced. Are you sure your instability is due to being off saturation as from my experience with a modern machine that is very rare?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: Karsten.Goemann-at-utas.edu.au [mailto:Karsten.Goemann-at-utas.edu.au] Sent: 23 June 2011 00:50 To: protrain-at-emcourses.com
Ken, Steve,
we're running our tungsten source SEM (no IGP, whole instrument vented and TP off during sample exchange...) in two modes:
Automated mineralogy, EDS mapping...: 40uA emission, fila tip way back in Wehnelt, not undersaturated as that seems to help beam stability, wait 1 hour for stabilisation after pump down and HV on. Filament heat setting at around 1.5-1.6V (not sure what temperature that corresponds to) after initial burn in. Filament lifetimes are between 700h and over 2000h, but usually over 1000h. Penning gauge usually reads mid E-4 Pa values during runs, ultimate pressure of the system around 5E-5 Pa.
Other SEM operation (biol. imaging, CL...): 100uA emission, fila tip much closer to grid cap, slightly undersaturated. Fila usually breaks at around 2 V after 40-150h, usually somewhere around 100h.
We have a second Wehnelt prepared in a desiccator for the other mode so we swap them, usually every 1-2 weeks. Fila lifetimes appear to be longer if one Wehnelt is in the SEM all the time, i.e. not swapped and stored externally.
Does this add some useful "data" to the oxidation / vac. quality vs. evaporation considerations? Cheers,
Karsten
-- Dr Karsten Goemann Research Fellow, Electron Microscopy & X-ray Microanalysis Central Science Laboratory, University of Tasmania Private Bag 74, Hobart TAS 7001, Australia Tel.: +61 (0) 3 6226 2146 (office & labs) Mobile: +61 (0) 407 101 990 Fax: +61 (0) 3 6226 2494 (general CSL) E-mail: Karsten.Goemann-at-utas.edu.au http://www.utas.edu.au/csl
} --------------------------------------------------------------------------- - } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 23 -- From Karsten.Goemann-at-utas.edu.au Wed Jun 22 18:49:24 2011 13, 23 -- Received: from mx2.utas.edu.au (ironport.its.utas.edu.au [131.217.6.11]) 13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MNnLh2013964 13, 23 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:49:22 -0500 13, 23 -- X-IronPort-AV: E=Sophos;i="4.65,409,1304258400"; 13, 23 -- d="scan'208";a="24609081" 13, 23 -- Received: from unknown (HELO mmp-sby1.its.utas.edu.au) ([10.10.3.71]) 13, 23 -- by ironport-outgoing.utas.edu.au with ESMTP; 23 Jun 2011 09:49:15 +1000 13, 23 -- MIME-version: 1.0 13, 23 -- Content-type: text/plain; charset=iso-8859-1; format=flowed 13, 23 -- Received: from [131.217.50.60] by mmp-sby1.its.utas.edu.au 13, 23 -- (Sun Java(tm) System Messaging Server 7u3-17.01 64bit (built Jun 8 2010)) 13, 23 -- with ESMTPA id {0LN700MP9U626660-at-mmp-sby1.its.utas.edu.au} for 13, 23 -- microscopy-at-microscopy.com; Thu, 23 Jun 2011 09:49:15 +1000 (EST) 13, 23 -- Message-id: {a06240801ca28292a0f77-at-[131.217.50.60]} 13, 23 -- In-reply-to: {201106221738.p5MHci6c026839-at-ns.microscopy.com} 13, 23 -- References: {201106221738.p5MHci6c026839-at-ns.microscopy.com} 13, 23 -- Date: Thu, 23 Jun 2011 09:49:29 +1000 13, 23 -- To: microscopy-at-microscopy.com 13, 23 -- From: Karsten Goemann {Karsten.Goemann-at-utas.edu.au} 13, 23 -- Subject: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 13, 23 -- Content-Transfer-Encoding: 8bit 13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MNnLh2013964 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 28 -- From protrain-at-emcourses.com Thu Jun 23 03:32:11 2011 28, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 28, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5N8W9ki022142 28, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 03:32:10 -0500 28, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:54202 helo=ProtrainPC) 28, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 28, 28 -- (envelope-from {protrain-at-emcourses.com} ) 28, 28 -- id 1QZfKV-0004Y4-T5 by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Thu, 23 Jun 2011 10:32:05 +0200 28, 28 -- Reply-To: {protrain-at-emcourses.com} 28, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 28, 28 -- To: {Karsten.Goemann-at-utas.edu.au} 28, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 28, 28 -- References: {201106222350.p5MNo2hB015168-at-ns.microscopy.com} 28, 28 -- In-Reply-To: {201106222350.p5MNo2hB015168-at-ns.microscopy.com} 28, 28 -- Subject: RE: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? 28, 28 -- Date: Thu, 23 Jun 2011 09:31:59 +0100 28, 28 -- Organization: Protrain 28, 28 -- Message-ID: {003c01cc3180$0635b620$12a12260$-at-com} 28, 28 -- MIME-Version: 1.0 28, 28 -- Content-Type: text/plain; 28, 28 -- charset="iso-8859-1" 28, 28 -- X-Mailer: Microsoft Office Outlook 12.0 28, 28 -- Thread-Index: AcwxNyfx77MKjlTvT2Kg/x3SF0A9VQAR7ZaQ 28, 28 -- Content-Language: en-gb 28, 28 -- X-Scan-Signature: 639da18d672d40a763013f2afc2ad59d{802}} 28, 28 -- X-Trace: smtp01.dial-up.net 1QZfKV-0004Y4-T5 af24a42e9a6c900f162b48e49324d6c5 28, 28 -- Content-Transfer-Encoding: 8bit 28, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5N8W9ki022142 ==============================End of - Headers==============================
In fact a tungstene filament can get a very long life if it is not saturate. This way is usable in SEM but not so much in TEM because light density of the spot may become irregular. Temperature of a tungsten filament increase quickly even when a few current eating is added that is the reason why it's very important for lifetime to adjust saturation just on the edge. There is many lifetime value of a tungstene filament almost one for each microscope and for each people who adjust saturation current. Vacuum is more important otherwise it cannot work well, then adjustment of current is also very important for lifetime. Working unsaturate can be done if the job is not precise (small magnification, big spot size, no low voltage, etc..) but may cause problems if you need resolution or very stable current. In our SEM, tungstene filament is changed every 3 month; we work 5 days per week with a saturate filament and under 4.10-6 mBar. Regards.
-- Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung
protrain-at-emcourses.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Ken } } I usually agree with your comments but this time I have to throw in a curved } ball. } } I often work with automated analysis SEMs which have a tungsten filament } life of upwards from 2500 hours; admittedly we run at 11 micro amps emission } with the filament well back. The instrument are not used by electron } microscopists so the idea behind this long life being that the service } technician is able to fit a new filament on their six monthly service } visits. } } We worked hard to obtain this life and one of the features that we found } that was very important in our development was the vacuum level. Thus we } run with an ion pump on the gun and the difference in filament life is } considerable. This is no criticism of the basic instrument, which exhibits a } gun vacuum level in its standard form the same if not better than its } competitors. } } Another area that the gun vacuum level influenced was the high voltage } stability. The instruments need to run for up to 24 hours unattended and it } is most important for the high voltage to remain absolutely stable over that } entire period of time. Double plots of high voltage level and gun vacuum } show that once the gun vacuum settles so does the high voltage. Thus the } operators wait for this stability period prior to starting a sample run; } proven to be the best practice. } } As a second point I know LaB6 users who have been forced to use tungsten } whilst waiting for a new source and they have also commented on the } increased filament life. } } Maybe I should say that not one of these comments relates to clients in the } United States; must be related to the type of air :-) } } Regards } } Steve } } Steve Chapman FRMS } Senior Consultant Protrain } For consultancy and training in electron microscopy world wide } Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 } www.emcourses.com } } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } Sent: 22 June 2011 16:07 } To: protrain-at-emcourses.com } Subject: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Roy, } I beg to differ. I have not seen any significant increase in tungsten } filament life in an ion-pumped gun versus a normal gun in good condition in } an SEM. Poor vacuum will definitely shorten a W filament's life, but a } better vacuum makes very little difference due to the fact that W filaments } run hotter than LaB6 and evaporate faster. The reason poor vacuum } accelerates their demise is due to oxidation on top of evaporation. } } No W filament I've ever seen has lasted more than about 300 hours at } operating temperature in an SEM, 10E-7T vacuum notwithstanding. My } observations may not hold for a TEM because TEMs run at lower } temperatures/emission currents and therefore evaporation rates should be } significantly lower. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] } Sent: Wednesday, June 22, 2011 10:31 AM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John. } } Like Nicolas said here below, it's all a matter of vacuum. } Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs } usually have a filament life of way above 1000 hours. If you take the same } SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get } way above 1000 hours with the tungsten filament. } } So, in theory if your SEM gun has a very good vacuum, you should be getting } a very high lifetime from each tungsten filament. If not, you could expect } to get good result with the LaB6 filemant, but keep the lifetime of your } current tungsten one. Keep in mind that the LaB6 filament is exponentially } more expensive than tungsten. } } It'd be best to consult with Jeol in any case before you decide to do this. } } Regards, } Roy } } -----Original Message----- } X-from: Nicolas.Stephant-at-univ-nantes.fr } [mailto:Nicolas.Stephant-at-univ-nantes.fr] } Sent: Thursday, June 16, 2011 1:45 PM } To: Roy Golombick } Subject: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John } } LaB6 filament have to work in a good vacuum. There is some instruments } like your TEM working with LaB6 in a gun pumped by DP or turbo, but most } of the LaB6 TEM work with high vacuum in the gun provided by SIP. If } your vacuum system is very good you may use LaB6 but you must understand } that you will work on an edge. Anyway, you may try without danger for } your instrument and you will see if LaB6 stay save during one year } (good) or one month. Please note that you may use another wehnelt cap } for this kind of filament and you have to change distance beetween } filament tip and wehnelt cap. First eating of this filament must be done } very slowy (about one hour) after almost one night of pumping. Working } with LaB6 is very comfortable because the brigtness is very high and it } may increase significantly top end result. } Regards } } Nicolas STEPHANT } } Université de Nantes } Institut Jean Rouxel } Service de microscopie électronique à balayage et microanalyse } 2 rue de la Houssinière } BP 92208 } 44322 Nantes cédex 3 } } Tél : 02 40 37 64 26 } Mél : nicolas.stephant-at-univ-nantes.fr } Web : http://www.univ-nantes.fr/smebm } } "Le monde n'existe que pour autant que nous sommes capables d'en } produire une image" } } C.G Jung } john.mitchels-at-gmail.com a écrit : } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } Hi Listers } } } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } } enough in which to use a LaB6. The engineers seem to have mix } } opinions, as does the great google. I would like comments from anyone } } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } } The microscope is behaving itself very well, no leaks everything is as } } good as it gets given its age. With one fitted do you notice any } } significant difference when working at the top end? } } } } Thanks in advance. } } } } John } } } } ==============================Original } } } Headers============================== } } } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com } } } [209.85.210.41]) } } } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } p5G8RUwL002495 } } } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 } } } -0500 } } } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 } } } 01:27:29 -0700 (PDT) } } } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 4, 30 -- d=gmail.com; s=gamma; } } 4, 30 -- } } } h=domainkey-signature:mime-version:date:message-id:subject:from:to } } } 4, 30 -- :content-type; } } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } } 4, 30 -- } } } b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } } } 4, 30 -- } } } fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } } } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 4, 30 -- d=gmail.com; s=gamma; } } 4, 30 -- } } } h=mime-version:date:message-id:subject:from:to:content-type; } } } 4, 30 -- } } } b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } } } 4, 30 -- } } } fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } } } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } } 4, 30 -- MIME-Version: 1.0 } } 4, 30 -- Received: by 10.68.51.201 with SMTP id } } } m9mr351917pbo.135.1308212849644; Thu, } } } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 } } } -0700 (PDT) } } } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } } 4, 30 -- To: Microscopy-at-microscopy.com } } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } } ==============================End of - } } } Headers============================== } } } } } } }
==============================Original Headers============================== 9, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 23 04:44:57 2011 9, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5N9iuGg007457 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 04:44:57 -0500 9, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 9, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5N9iskV021506 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 11:44:54 +0200 9, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 9, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5N9iq63002130 9, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 11:44:52 +0200 9, 25 -- Message-ID: {4E030B20.2060800-at-univ-nantes.fr} 9, 25 -- Date: Thu, 23 Jun 2011 11:45:04 +0200 9, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 9, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 9, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 9, 25 -- MIME-Version: 1.0 9, 25 -- To: Microscopy-at-microscopy.com 9, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 9, 25 -- References: {201106221658.p5MGw4Qp014948-at-ns.microscopy.com} 9, 25 -- In-Reply-To: {201106221658.p5MGw4Qp014948-at-ns.microscopy.com} 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-Miltered: at b-mail with ID 4E030B16.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 9, 25 -- X-j-chkmail-Enveloppe: 4E030B16.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Stephant-at-univ-nantes.fr} ==============================End of - Headers==============================
I am interested in your comment regarding the TEM. As a ex demonstrator for Hitachi, JEOL and ISI we always ran TEM just under saturated for a reason.
If you operate a TEM with the condenser at crossover you visualise the virtual source. It is a very poor operating technique if you do this because the illumination is at its least coherent! Why demonstrators (and I teach people) run under saturated is that at high magnifications you would not see that you are in this poor operating condition if you did not sense the shadows from imaging the under saturated filament.
What I teach is to operate with some dark patches within the filament image. At low magnifications you are able to see when you are at condenser crossover and are able to over focus the illumination to increase the coherence. At high magnification as mentioned above you sense the uneven illumination at crossover so you know that from that point you should move over focus to improve coherence/performance.
As far as I am concerned these are fundamentals of TEM operation. Just take an image with the illumination near crossover and then repeat with the illumination further over focus and you will see my point. If you are unable to see a difference in the image quality it indicates to me that the camera you are using has insufficient resolution. Be fortunate enough to still use film and you will clearly see the difference.
I hope this helps?
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: Nicolas.Stephant-at-univ-nantes.fr [mailto:Nicolas.Stephant-at-univ-nantes.fr] Sent: 23 June 2011 10:46 To: protrain-at-emcourses.com
Hi listeners
In fact a tungstene filament can get a very long life if it is not saturate. This way is usable in SEM but not so much in TEM because light density of the spot may become irregular. Temperature of a tungsten filament increase quickly even when a few current eating is added that is the reason why it's very important for lifetime to adjust saturation just on the edge. There is many lifetime value of a tungstene filament almost one for each microscope and for each people who adjust saturation current. Vacuum is more important otherwise it cannot work well, then adjustment of current is also very important for lifetime. Working unsaturate can be done if the job is not precise (small magnification, big spot size, no low voltage, etc..) but may cause problems if you need resolution or very stable current. In our SEM, tungstene filament is changed every 3 month; we work 5 days per week with a saturate filament and under 4.10-6 mBar. Regards.
-- Nicolas STEPHANT
Université de Nantes Institut Jean Rouxel Service de microscopie électronique à balayage et microanalyse 2 rue de la Houssinière BP 92208 44322 Nantes cédex 3
"Le monde n'existe que pour autant que nous sommes capables d'en produire une image"
C.G Jung
protrain-at-emcourses.com a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Ken } } I usually agree with your comments but this time I have to throw in a curved } ball. } } I often work with automated analysis SEMs which have a tungsten filament } life of upwards from 2500 hours; admittedly we run at 11 micro amps emission } with the filament well back. The instrument are not used by electron } microscopists so the idea behind this long life being that the service } technician is able to fit a new filament on their six monthly service } visits. } } We worked hard to obtain this life and one of the features that we found } that was very important in our development was the vacuum level. Thus we } run with an ion pump on the gun and the difference in filament life is } considerable. This is no criticism of the basic instrument, which exhibits a } gun vacuum level in its standard form the same if not better than its } competitors. } } Another area that the gun vacuum level influenced was the high voltage } stability. The instruments need to run for up to 24 hours unattended and it } is most important for the high voltage to remain absolutely stable over that } entire period of time. Double plots of high voltage level and gun vacuum } show that once the gun vacuum settles so does the high voltage. Thus the } operators wait for this stability period prior to starting a sample run; } proven to be the best practice. } } As a second point I know LaB6 users who have been forced to use tungsten } whilst waiting for a new source and they have also commented on the } increased filament life. } } Maybe I should say that not one of these comments relates to clients in the } United States; must be related to the type of air :-) } } Regards } } Steve } } Steve Chapman FRMS } Senior Consultant Protrain } For consultancy and training in electron microscopy world wide } Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 } www.emcourses.com } } } } -----Original Message----- } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz] } Sent: 22 June 2011 16:07 } To: protrain-at-emcourses.com } Subject: [Microscopy] RE: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Roy, } I beg to differ. I have not seen any significant increase in tungsten } filament life in an ion-pumped gun versus a normal gun in good condition in } an SEM. Poor vacuum will definitely shorten a W filament's life, but a } better vacuum makes very little difference due to the fact that W filaments } run hotter than LaB6 and evaporate faster. The reason poor vacuum } accelerates their demise is due to oxidation on top of evaporation. } } No W filament I've ever seen has lasted more than about 300 hours at } operating temperature in an SEM, 10E-7T vacuum notwithstanding. My } observations may not hold for a TEM because TEMs run at lower } temperatures/emission currents and therefore evaporation rates should be } significantly lower. } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: Roy-at-picotech.co.il [mailto:Roy-at-picotech.co.il] } Sent: Wednesday, June 22, 2011 10:31 AM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John. } } Like Nicolas said here below, it's all a matter of vacuum. } Because of the well pumped gun (usually turbo or ion pumped), LaB6 SEMs } usually have a filament life of way above 1000 hours. If you take the same } SEM and exchange the LaB6 filament for a Tungsten one, you'll be able to get } way above 1000 hours with the tungsten filament. } } So, in theory if your SEM gun has a very good vacuum, you should be getting } a very high lifetime from each tungsten filament. If not, you could expect } to get good result with the LaB6 filemant, but keep the lifetime of your } current tungsten one. Keep in mind that the LaB6 filament is exponentially } more expensive than tungsten. } } It'd be best to consult with Jeol in any case before you decide to do this. } } Regards, } Roy } } -----Original Message----- } X-from: Nicolas.Stephant-at-univ-nantes.fr } [mailto:Nicolas.Stephant-at-univ-nantes.fr] } Sent: Thursday, June 16, 2011 1:45 PM } To: Roy Golombick } Subject: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi John } } LaB6 filament have to work in a good vacuum. There is some instruments } like your TEM working with LaB6 in a gun pumped by DP or turbo, but most } of the LaB6 TEM work with high vacuum in the gun provided by SIP. If } your vacuum system is very good you may use LaB6 but you must understand } that you will work on an edge. Anyway, you may try without danger for } your instrument and you will see if LaB6 stay save during one year } (good) or one month. Please note that you may use another wehnelt cap } for this kind of filament and you have to change distance beetween } filament tip and wehnelt cap. First eating of this filament must be done } very slowy (about one hour) after almost one night of pumping. Working } with LaB6 is very comfortable because the brigtness is very high and it } may increase significantly top end result. } Regards } } Nicolas STEPHANT } } Université de Nantes } Institut Jean Rouxel } Service de microscopie électronique à balayage et microanalyse } 2 rue de la Houssinière } BP 92208 } 44322 Nantes cédex 3 } } Tél : 02 40 37 64 26 } Mél : nicolas.stephant-at-univ-nantes.fr } Web : http://www.univ-nantes.fr/smebm } } "Le monde n'existe que pour autant que nous sommes capables d'en } produire une image" } } C.G Jung } john.mitchels-at-gmail.com a écrit : } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } Hi Listers } } } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } } enough in which to use a LaB6. The engineers seem to have mix } } opinions, as does the great google. I would like comments from anyone } } who has or has had a 1200 fitted with a LaB6. If you have, which ones? } } The microscope is behaving itself very well, no leaks everything is as } } good as it gets given its age. With one fitted do you notice any } } significant difference when working at the top end? } } } } Thanks in advance. } } } } John } } } } ==============================Original } } } Headers============================== } } } 4, 30 -- From john.mitchels-at-gmail.com Thu Jun 16 03:27:31 2011 } } 4, 30 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com } } } [209.85.210.41]) } } } 4, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } } p5G8RUwL002495 } } } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 03:27:30 } } } -0500 } } } 4, 30 -- Received: by pzk4 with SMTP id 4so1148803pzk.0 } } 4, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Jun 2011 } } } 01:27:29 -0700 (PDT) } } } 4, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 4, 30 -- d=gmail.com; s=gamma; } } 4, 30 -- } } } h=domainkey-signature:mime-version:date:message-id:subject:from:to } } } 4, 30 -- :content-type; } } 4, 30 -- bh=BmLtXz/tyRHLIvsvSIwdgbBBfxSaqB7Xad59EJb4v/k=; } } 4, 30 -- } } } b=j1MYi0tcYKKF68uwwH5zONhnFgNpDjzNzFAi3jgXgzZWbu7ZPXXXLwoNzAUhMlH83f } } } 4, 30 -- } } } fThWmJksuzPWZG9ODcDxCOXXmWDS5atq+8Cs0jAs1uzFvXLCPaHxtvLbyadIwk2QIQIA } } } 4, 30 -- b/GJMaDfLQd4/icDX67NiicZEc7RLac9Ni/I4= } } 4, 30 -- DomainKey-Signature: a=rsa-sha1; c=nofws; } } 4, 30 -- d=gmail.com; s=gamma; } } 4, 30 -- } } } h=mime-version:date:message-id:subject:from:to:content-type; } } } 4, 30 -- } } } b=uMAyhiBE6bnMecvG2bwYbFXs+ivleMZ8NvGhtKqq/ZzrXv+96nlgGHIPlNfuNlOfMy } } } 4, 30 -- } } } fDsR6sltJNEJZYH48CpJ1BSi3xvo/BbC4HUUxjAy1DWXg2+nBmEIJi/bGcmuK5qjgUfi } } } 4, 30 -- Ft7TTRPVLp6ZhILauXV+eaPlsrdyEQTVAQuQs= } } 4, 30 -- MIME-Version: 1.0 } } 4, 30 -- Received: by 10.68.51.201 with SMTP id } } } m9mr351917pbo.135.1308212849644; Thu, } } } 4, 30 -- 16 Jun 2011 01:27:29 -0700 (PDT) } } 4, 30 -- Received: by 10.68.44.5 with HTTP; Thu, 16 Jun 2011 01:27:29 } } } -0700 (PDT) } } } 4, 30 -- Date: Thu, 16 Jun 2011 09:27:29 +0100 } } 4, 30 -- Message-ID: {BANLkTi=ReDJ8FYz_b+NXqTe6CnijZeSs5w-at-mail.gmail.com} } } 4, 30 -- Subject: LaB6 in a Jeol 1200ex2 is it possible? } } 4, 30 -- From: John Mitchels {john.mitchels-at-gmail.com} } } 4, 30 -- To: Microscopy-at-microscopy.com } } 4, 30 -- Content-Type: text/plain; charset=ISO-8859-1 } } ==============================End of - } } } Headers============================== } } } } } } }
==============================Original Headers============================== 9, 25 -- From Nicolas.Stephant-at-univ-nantes.fr Thu Jun 23 04:44:57 2011 9, 25 -- Received: from mail.cnrs-imn.fr (mail.cnrs-imn.fr [193.52.97.14]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5N9iuGg007457 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 04:44:57 -0500 9, 25 -- Received: from i-smtp.cnrs-imn.fr (smtp.cnrs-imn.fr [194.214.57.230]) 9, 25 -- by mail.cnrs-imn.fr (8.14.3/8.14.3/DG) with ESMTP id p5N9iskV021506 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 11:44:54 +0200 9, 25 -- Received: from [10.2.13.180] (p180-nom.cnrs-imn.fr [10.2.13.180]) 9, 25 -- by i-smtp.cnrs-imn.fr (8.13.8/8.13.8/Debian-3) with ESMTP id p5N9iq63002130 9, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 9, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 11:44:52 +0200 9, 25 -- Message-ID: {4E030B20.2060800-at-univ-nantes.fr} 9, 25 -- Date: Thu, 23 Jun 2011 11:45:04 +0200 9, 25 -- From: Nicolas Stephant {Nicolas.Stephant-at-univ-nantes.fr} 9, 25 -- Reply-To: Nicolas.Stephant-at-univ-nantes.fr 9, 25 -- User-Agent: Thunderbird 2.0.0.6 (Windows/20070728) 9, 25 -- MIME-Version: 1.0 9, 25 -- To: Microscopy-at-microscopy.com 9, 25 -- Subject: Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 9, 25 -- References: {201106221658.p5MGw4Qp014948-at-ns.microscopy.com} 9, 25 -- In-Reply-To: {201106221658.p5MGw4Qp014948-at-ns.microscopy.com} 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-Miltered: at b-mail with ID 4E030B16.000 by Joe's j-chkmail (http : // j-chkmail dot ensmp dot fr)! 9, 25 -- X-j-chkmail-Enveloppe: 4E030B16.000/194.214.57.230/smtp.cnrs-imn.fr/i-smtp.cnrs-imn.fr/ {Nicolas.Ste phant-at-univ-nantes.fr} ==============================End of - Headers==============================
==============================Original Headers============================== 27, 28 -- From protrain-at-emcourses.com Thu Jun 23 06:29:58 2011 27, 28 -- Received: from smtp01.dial-up.net (smtp01.dial-up.net [196.26.208.170]) 27, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5NBTtss025205 27, 28 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 06:29:56 -0500 27, 28 -- Received: from 5ac8bf09.bb.sky.com ([90.200.191.9]:54807 helo=ProtrainPC) 27, 28 -- by smtp01.dial-up.net with esmtpa (Exim 4.76 #0) 27, 28 -- (envelope-from {protrain-at-emcourses.com} ) 27, 28 -- id 1QZi6X-000KcH-Ee by authid {09b79efaf87c50cb314d7cc58a4aab80} with fixed_login; Thu, 23 Jun 2011 13:29:51 +0200 27, 28 -- Reply-To: {protrain-at-emcourses.com} 27, 28 -- From: "Steve Chapman" {protrain-at-emcourses.com} 27, 28 -- To: {Nicolas.Stephant-at-univ-nantes.fr} 27, 28 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 27, 28 -- References: {201106230945.p5N9jqHU008661-at-ns.microscopy.com} 27, 28 -- In-Reply-To: {201106230945.p5N9jqHU008661-at-ns.microscopy.com} 27, 28 -- Subject: RE: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 27, 28 -- Date: Thu, 23 Jun 2011 12:29:42 +0100 27, 28 -- Organization: Protrain 27, 28 -- Message-ID: {007501cc3198$db5ac210$92104630$-at-com} 27, 28 -- MIME-Version: 1.0 27, 28 -- Content-Type: text/plain; 27, 28 -- charset="iso-8859-1" 27, 28 -- X-Mailer: Microsoft Office Outlook 12.0 27, 28 -- Thread-Index: AcwximSaL79hfBDESaqH8ZQLS8+D1gAB/RVA 27, 28 -- Content-Language: en-gb 27, 28 -- X-Scan-Signature: ee4878f3577b28b052e17883476015f7{529}} 27, 28 -- X-Trace: smtp01.dial-up.net 1QZi6X-000KcH-Ee 946705cdfb4112793d44b262c22ad744 27, 28 -- Content-Transfer-Encoding: 8bit 27, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5NBTtss025205 ==============================End of - Headers==============================
Yes, we also have a JEOL 1200EXII with stacked DP, and running LaB6 (Gun vacuum runs below 1 x10-4 Pa the gage pegs there). We recently inherited the scope so I do not have LaB6 life expectancy. It came with the LaB6 installed and I know that it has been running LaB6 for years. I am happy with Peter gets 900-1500 hours.
I was horrified to have the LaB6 initially fearing its fragility. But it has survived 7 months including training 10 brand new TEM students! (New to EM not just this TEM).
For some maintenance issues (allowing rapid filament startups) and for testing we recently switched to a Tungsten filament. Tungsten is fine below 150Kx but brightness really becomes and issue 100K - 500K.
So here's a question for the list. I noted on our new JEOL 2100 with a LaB6, running at 80Kv or 100Kv imaging on the Phos Screen appears "dim" (aligned and operating properly). I compared this to a JEOL 100S Tungsten, and JEOL 100CXII Tungsten, and Zeiss 10C Tungsten. All these appear brighter at 80 & 100Kv. So my assumpution was: (1) the Phos mixture on the 2100 screen was different to accomdate the 200Kv or (2) the tigher alignments (and 3 Cond Lenes) to gain higher resolution removed more electrons from the beam, effectively reducing the beam current down the column. But I have noted the same reduced brightness on the 1200EXII and does not have the 3 CL's only 2CL's. In talking with some service engineers they noted the same thing newer scopes appear to be "dimmer" to human eyes. One mentioned the a posible assumption that everyone uses digital cameras these days so losing more beam current isn't an issue. However, the 1200 series was before ubiquity of digital cameras (early 90's). And yes, I have taken into account worn out beat up screens and have compared in fact worn out screen (100s) and brand new coated screens (10C & 2100) and good/ excellent condition 100CXII & 1200) - so I do not believe it is wear and tear on the screens. BUT could it be changes in the Phos-mixture on the screens? Or something else?
Any one have any comments?
Please I do not want to start a brand war. I have not had the chance to spend siognificant time on FEI or Hitachi Scopes to fairly judge their brightness.
On 16 Jun 2011 at 4:28, john.mitchels-at-gmail.com wrote:
} } } } -------------------------------------------------------------------- } -- ------ The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------- } -- ------ } } Hi Listers } } We have a Jeol 1200 EXII TEM and we were wondering if it is good } enough in which to use a LaB6. The engineers seem to have mix } opinions, as does the great google. I would like comments from } anyone who has or has had a 1200 fitted with a LaB6. If you have, } which ones? The microscope is behaving itself very well, no leaks } everything is as good as it gets given its age. With one fitted do } you notice any significant difference when working at the top end? } } Thanks in advance. } } John } } ==============================Original } Headers============================== 4, 30 -- From
Richard E. Edelmann, Ph.D. EXPO Editor, Microscopy and Microanalysis Supplement Director, Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-muohio.edu http://www.cami.muohio.edu
==============================Original Headers============================== 18, 23 -- From edelmare-at-muohio.edu Thu Jun 23 07:44:08 2011 18, 23 -- Received: from mualmarp02.mcs.muohio.edu (mualmarp02.mcs.muohio.edu [134.53.6.74]) 18, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5NCi71C010170 18, 23 -- for {microscopy-at-Microscopy.com} ; Thu, 23 Jun 2011 07:44:08 -0500 18, 23 -- Received: from mualmafp02.mcs.muohio.edu (mualmafp02.mcs.muohio.edu [134.53.6.6]) 18, 23 -- by mualmarp02.mcs.muohio.edu (8.13.8/8.13.8) with ESMTP id p5NCi74H022530 18, 23 -- for {microscopy-at-Microscopy.com} ; Thu, 23 Jun 2011 08:44:07 -0400 18, 23 -- Received: from [10.34.160.232] ([10.34.160.232]) 18, 23 -- by mualmafp02.mcs.muohio.edu (8.13.8/8.13.8) with ESMTP id p5NCi72t024205 18, 23 -- for {microscopy-at-Microscopy.com} ; Thu, 23 Jun 2011 08:44:07 -0400 18, 23 -- From: "Richard E. Edelmann" {edelmare-at-muohio.edu} 18, 23 -- To: microscopy-at-Microscopy.com 18, 23 -- Date: Thu, 23 Jun 2011 08:44:07 -0400 18, 23 -- MIME-Version: 1.0 18, 23 -- Subject: (Fwd) Re: [Microscopy] LaB6 in a Jeol 1200ex2 is it possible? 18, 23 -- Message-ID: {4E02FCD7.20628.4817940D-at-edelmare.muohio.edu} 18, 23 -- X-Confirm-Reading-To: "Richard E. Edelmann" {edelmare-at-muohio.edu} 18, 23 -- X-pmrqc: 1 18, 23 -- Priority: normal 18, 23 -- X-mailer: Pegasus Mail for Windows (4.41) 18, 23 -- Content-type: text/plain; charset=US-ASCII 18, 23 -- Content-transfer-encoding: 7BIT 18, 23 -- Content-description: Mail message body ==============================End of - Headers==============================
The reviewer should visit the electron microscope lab at Western Digital Media. All the samples are at least slightly magnetic, because that is all they work on. I am sure the same is true at Seagate, Hitachi and every university that does research on magnetic materials.
A. John Mardinly, ASU
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Wednesday, June 22, 2011 3:21 PM To: John Mardinly
Hi all,
We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the spectra. I understood that these elements are from the polepiece and other components in the sample area.
When a student submitted a manuscript with these elements in the spectrum, along with the other elements of interest (IN, Sb), a reviewer said that the TEM instruments are strictly restricted from using the magnetic impurities/parts, so there must be other sources for iron.
Comments? How would you respond to this reviewer.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 11, 28 -- From dsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 11, 28 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5MMDw5g013430 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu [172.21.6.90]) 11, 28 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB01F.purdue.lcl 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 11, 28 -- Subject: Tem EDX 11, 28 -- Thread-Topic: Tem EDX 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} 11, 28 -- Accept-Language: en-US 11, 28 -- Content-Language: en-US 11, 28 -- X-MS-Has-Attach: 11, 28 -- X-MS-TNEF-Correlator: 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 11, 28 -- acceptlanguage: en-US 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" 11, 28 -- MIME-Version: 1.0 11, 28 -- X-PMX-Version: 5.5.9.388399 11, 28 -- X-PerlMx-Virus-Scanned: Yes 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5MMDw5g013430 ==============================End of - Headers==============================
==============================Original Headers============================== 19, 40 -- From John.Mardinly-at-asu.edu Thu Jun 23 15:38:46 2011 19, 40 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 19, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5NKckNf002235 19, 40 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 15:38:46 -0500 19, 40 -- X-ASG-Debug-ID: 1308861525-11b3b8760001-4CH8be 19, 40 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet6.asu.edu with ESMTP id 5F8FUhYtj0Bvjmxe (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Thu, 23 Jun 2011 13:38:45 -0700 (MST) 19, 40 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 19, 40 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 19, 40 -- X-ASG-Whitelist: Client 19, 40 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.196) by 19, 40 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 19, 40 -- 8.1.436.0; Thu, 23 Jun 2011 13:38:45 -0700 19, 40 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by 19, 40 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 23 Jun 19, 40 -- 2011 13:38:45 -0700 19, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 19, 40 -- Content-Class: urn:content-classes:message 19, 40 -- MIME-Version: 1.0 19, 40 -- Content-Type: text/plain; charset="iso-8859-1" 19, 40 -- Subject: RE: [Microscopy] Tem EDX 19, 40 -- Date: Thu, 23 Jun 2011 13:38:44 -0700 19, 40 -- X-ASG-Orig-Subj: RE: [Microscopy] Tem EDX 19, 40 -- Message-ID: {95FFD84122D92D4F913DE75095E9C42E06B900FB-at-EX05.asurite.ad.asu.edu} 19, 40 -- In-Reply-To: {201106222220.p5MMKmeu028904-at-ns.microscopy.com} 19, 40 -- X-MS-Has-Attach: 19, 40 -- X-MS-TNEF-Correlator: 19, 40 -- Thread-Topic: [Microscopy] Tem EDX 19, 40 -- Thread-Index: AcwxKqSvnV6RBWaxTVe/3jG6aDPWWwAuo3UQ 19, 40 -- References: {201106222220.p5MMKmeu028904-at-ns.microscopy.com} 19, 40 -- From: John Mardinly {John.Mardinly-at-asu.edu} 19, 40 -- To: {dsherman-at-purdue.edu} 19, 40 -- CC: {microscopy-at-microscopy.com} 19, 40 -- X-OriginalArrivalTime: 23 Jun 2011 20:38:45.0049 (UTC) FILETIME=[8BD9F290:01CC31E5] 19, 40 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 19, 40 -- X-Barracuda-Start-Time: 1308861525 19, 40 -- X-Barracuda-Encrypted: RC4-MD5 19, 40 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 19, 40 -- X-Virus-Scanned: by bsmtpd at asu.edu 19, 40 -- Content-Transfer-Encoding: 8bit 19, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5NKckNf002235 ==============================End of - Headers==============================
While the Fe, Co etc are undoubtedly from the pole-piece, I wonder why you see such a significant contribution to the spectrum. As long as the objective aperture is out, even the scattered electrons should go down the bore of the pole-piece. Brehmstrahlung from the condenser apertures may have some effect, but it should be small.
Analytical scopes have improved dramatically over the years and I thought stray signal was pretty much a thing of the past. Pretty much every microscope I've used in the past 25 years has almost no x-rays from the p-p. Do you have an unusual scope? (You can reply off-line if you don't wish to identify the scope publicly.) In my experience, the EDX collimators on TEM detectors don't shield very much, so the improvements in stray signal have been due to changes in the column itself.
Cheers, Henk
At 6/22/2011 8:32 PM, dsherman-at-purdue.edu wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Thanks to all for the quick response. Consensus is that the reviewer is } incorrect as many scopes do see Fe and Co in EDX spectra from the polepiece. } The amount may depend on the type of microscope, detector, etc but } apparently many instruments show this. } } I didn't mention this in my first E-mail but I regularly take a background } spectrum and they invariably show Fe and Co in our instrument. It is } difficult to do a background subtraction and remove all of it. Presumably } this is because the sample does deflect many more electrons than just the } support film resulting in more X-ray production from other areas within the } objective lens. } } We also have a cryo blade that is made of copper and also the connection } from the external nitrogen dewar to the decontaminator is a copper braid. } Thus, even when using a low background (Beryllium) holder and nickel grid we } often get a small amount of Cu. This may not show up with a SiLi detector } but we get so many counts with the SDD that this may translate into higher } background peaks for some of these elements. } } Debby } } } On 6/22/11 6:15 PM, "Debby Sherman" {dsherman-at-purdue.edu} wrote: } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi all, } } } } We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the } } spectra. I understood that these elements are from the polepiece and other } } components in the sample area. } } } } When a student submitted a manuscript with these elements in the spectrum, } } along with the other elements of interest (IN, Sb), a reviewer said that } } the TEM instruments are strictly restricted from using the magnetic } } impurities/parts, so there must be other sources for iron. } } } } Comments? How would you respond to this reviewer. } } } } Debby } } } } --- } } Debby Sherman, Director Phone: 765-494-6666 } } Life Science Microscopy Facility FAX: 765-494-5896 } } Purdue University E-mail:dsherman-at-purdue.edu } } S-052 Whistler Building } } 170 S. University Street } } West Lafayette, IN 47907 } } http://www.ag.purdue.edu/facilities/microscopy } } } } } } } } } } } } } } ==============================Original Headers============================== } } 11, 28 -- Fromdsherman-at-purdue.edu Wed Jun 22 17:13:58 2011 } } 11, 28 -- Received: from mailhub128.itcs.purdue.edu } } (mailhub128.itcs.purdue.edu [128.210.5.128]) } } 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p5MMDw5g013430 } } 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 17:13:58 -0500 } } 11, 28 -- Received: from WPPEXHUB01F.purdue.lcl (wppexhub01f.itap.purdue.edu } } [172.21.6.90]) } } 11, 28 -- by mailhub128.itcs.purdue.edu } } (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5MMDvIH016569 } } 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 18:13:57 -0400 } } 11, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by } } WPPEXHUB01F.purdue.lcl } } 11, 28 -- ([::1]) with mapi; Wed, 22 Jun 2011 18:13:57 -0400 } } 11, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } } 11, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } } 11, 28 -- Date: Wed, 22 Jun 2011 18:13:53 -0400 } } 11, 28 -- Subject: Tem EDX } } 11, 28 -- Thread-Topic: Tem EDX } } 11, 28 -- Thread-Index: AcwxKauksR1Q2TqPW0qILO91Ge7kCw== } } 11, 28 -- Message-ID: {CA27E161.F54A%dsherman-at-purdue.edu} } } 11, 28 -- Accept-Language: en-US } } 11, 28 -- Content-Language: en-US } } 11, 28 -- X-MS-Has-Attach: } } 11, 28 -- X-MS-TNEF-Correlator: } } 11, 28 -- user-agent: Microsoft-Entourage/13.8.0.101117 } } 11, 28 -- acceptlanguage: en-US } } 11, 28 -- Content-Type: text/plain; charset="iso-8859-1" } } 11, 28 -- MIME-Version: 1.0 } } 11, 28 -- X-PMX-Version: 5.5.9.388399 } } 11, 28 -- X-PerlMx-Virus-Scanned: Yes } } 11, 28 -- Content-Transfer-Encoding: 8bit } } 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } } ns.microscopy.com id p5MMDw5g013430 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 9, 29 -- Fromdsherman-at-purdue.edu Wed Jun 22 19:30:48 2011 } 9, 29 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) } 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5N0Um1s030596 } 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 19:30:48 -0500 } 9, 29 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) } 9, 29 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p5N0UlfB014850 } 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 22 Jun 2011 20:30:47 -0400 } 9, 29 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.244]) by WPPEXHUB04H.purdue.lcl } 9, 29 -- ([::1]) with mapi; Wed, 22 Jun 2011 20:30:47 -0400 } 9, 29 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 9, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} } 9, 29 -- Date: Wed, 22 Jun 2011 20:30:43 -0400 } 9, 29 -- Subject: Re: [Microscopy] Tem EDX } 9, 29 -- Thread-Topic: [Microscopy] Tem EDX } 9, 29 -- Thread-Index: AcwxKc+ZrMVcwD1+REutdyv38FFgNQAEvmXQ } 9, 29 -- Message-ID: {CA280173.F573%dsherman-at-purdue.edu} } 9, 29 -- In-Reply-To: {201106222215.p5MMF71e016120-at-ns.microscopy.com} } 9, 29 -- Accept-Language: en-US } 9, 29 -- Content-Language: en-US } 9, 29 -- X-MS-Has-Attach: } 9, 29 -- X-MS-TNEF-Correlator: } 9, 29 -- user-agent: Microsoft-Entourage/13.8.0.101117 } 9, 29 -- acceptlanguage: en-US } 9, 29 -- Content-Type: text/plain; charset="iso-8859-1" } 9, 29 -- MIME-Version: 1.0 } 9, 29 -- X-PMX-Version: 5.5.9.388399 } 9, 29 -- X-PerlMx-Virus-Scanned: Yes } 9, 29 -- Content-Transfer-Encoding: 8bit } 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5N0Um1s030596 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 8, 26 -- From colijn.1-at-osu.edu Thu Jun 23 16:25:56 2011 8, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 8, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5NLPu5E018959 8, 26 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 16:25:56 -0500 8, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 8, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 26 -- id {01O2TWR91CTS9I6GRV-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 8, 26 -- Thu, 23 Jun 2011 17:25:54 -0400 (EDT) 8, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 8, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 26 -- with ESMTPA id {01O2TWR9RWUU9ER1R5-at-ecr6.ohio-state.edu} ; Thu, 8, 26 -- 23 Jun 2011 17:25:55 -0400 (EDT) 8, 26 -- Date: Thu, 23 Jun 2011 17:25:55 -0400 8, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 8, 26 -- Subject: Re: [Microscopy] Re: Tem EDX 8, 26 -- In-reply-to: {201106230032.p5N0WCZA032485-at-ns.microscopy.com} 8, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 8, 26 -- To: dsherman-at-purdue.edu, microscopy-at-microscopy.com 8, 26 -- Message-id: {4E03AF63.4000301-at-osu.edu} 8, 26 -- MIME-version: 1.0 8, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 26 -- Content-transfer-encoding: 7bit 8, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) 8, 26 -- Gecko/20110616 Thunderbird/3.1.11 8, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 8, 26 -- References: {201106230032.p5N0WCZA032485-at-ns.microscopy.com} ==============================End of - Headers==============================
We haven't spent a lot of time on quantifying this so 45 minutes might be enough. But we're loading up to 16 thick polished rock mounts together, so the time to achieve good vacuum can also vary a little. 1 hour certainly works and we can use the time to set up the run, do surveys of the specimens...
Is the filament heat current not still drifting during the HV tank stabilisation? I guess we could try saturated vs. undersaturated stabilisation with some sort of reproducible conditions (e.g. same pressure before venting, same duration vented, pump down without specimens installed...). Or constantly have a picoammeter hooked up and monitor the probe current during stabilisation.
Optimising this further wasn't high priority so far as overall stability and filament lifetimes are good (current one still going after over 2000h).
Cheers,
Karsten
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 11, 23 -- From Karsten.Goemann-at-utas.edu.au Thu Jun 23 17:47:58 2011 11, 23 -- Received: from mx2.utas.edu.au (ironport.its.utas.edu.au [131.217.6.11]) 11, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5NMluRN003520 11, 23 -- for {microscopy-at-microscopy.com} ; Thu, 23 Jun 2011 17:47:57 -0500 11, 23 -- X-IronPort-AV: E=Sophos;i="4.65,415,1304258400"; 11, 23 -- d="scan'208";a="24675929" 11, 23 -- Received: from unknown (HELO mmp-sby2.its.utas.edu.au) ([10.10.3.73]) 11, 23 -- by ironport-outgoing.utas.edu.au with ESMTP; 24 Jun 2011 08:47:55 +1000 11, 23 -- MIME-version: 1.0 11, 23 -- Content-type: text/plain; charset=iso-8859-1; format=flowed 11, 23 -- Received: from [10.1.1.3] ([131.217.240.14]) by mmp-sby2.its.utas.edu.au 11, 23 -- (Sun Java(tm) System Messaging Server 7u3-17.01 64bit (built Jun 8 2010)) 11, 23 -- with ESMTPA id {0LN900046LZS4VE0-at-mmp-sby2.its.utas.edu.au} for 11, 23 -- microscopy-at-microscopy.com; Fri, 24 Jun 2011 08:47:55 +1000 (EST) 11, 23 -- Message-id: {a06240800ca28cf4674a8-at-[10.1.1.3]} 11, 23 -- In-reply-to: {201106230838.p5N8cDiT029346-at-ns.microscopy.com} 11, 23 -- References: {201106230838.p5N8cDiT029346-at-ns.microscopy.com} 11, 23 -- Date: Fri, 24 Jun 2011 08:47:51 +1000 11, 23 -- To: microscopy-at-microscopy.com 11, 23 -- From: Karsten Goemann {Karsten.Goemann-at-utas.edu.au} 11, 23 -- Subject: [Microscopy] Re: LaB6 in a Jeol 1200ex2 is it possible? 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5NMluRN003520 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Mathew_1980-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I'm new at SEM EDX. At my new place we have a jeol jsm 6460lv with capability for both LaB6 and tungsten filaments. Most of the EDX work done here was made using the W anode; however, nobody can really explain why and that is my question: -For SEM EDX at magnifications of ~ 5000x, what source is better LaB6 or W filament? Why?
In the process of acquiring a new SEM the JEOL InTouchScope is up for consideration. Has any one encountered this tool that is willing to share their thoughts (on/off list)? We are particularly curious about the performance of the SE detector for LV. And can EDS really be simplified for the uninitiated to obtain accurate results?
We routinely process biological specimens for pathological diagnosis. Eg. Hair, tissue (incl teeth & bone), and occasional prosthetics.
Thanks in advance Naomi
Naomi McCallum Supervising Scientist, EM Unit, Anatomical Pathology Pathology Queensland Australia
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 10, 25 -- From prvs=11569584eb=naomi_mccallum-at-health.qld.gov.au Fri Jun 24 02:18:47 2011 10, 25 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5O7IkcE009947 10, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 02:18:47 -0500 10, 25 -- Received: from gwd-mail05.remote.health.qld.gov.au (gwd-mail05.remote.health.qld.gov.au [192.168.3.52]) 10, 25 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id p5O7IUa2008441 10, 25 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 17:18:31 +1000 10, 25 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 10, 25 -- gwd-mail05.remote.health.qld.gov.au (Queensland Health SMTP Server) 10, 25 -- with ESMTP id 10, 25 -- {T9dcf11b984c0a80334304-at-gwd-mail05.remote.health.qld.gov.au} for 10, 25 -- {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 17:18:30 +1000 10, 25 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 10, 25 -- Novell_GroupWise; Fri, 24 Jun 2011 17:18:30 +1000 10, 25 -- Message-Id: {4E04C6E0.88BE.00AA.1-at-health.qld.gov.au} 10, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 10, 25 -- Date: Fri, 24 Jun 2011 17:18:26 +1000 10, 25 -- From: "Naomi McCallum" {naomi_mccallum-at-health.qld.gov.au} 10, 25 -- To: {Microscopy-at-microscopy.com} 10, 25 -- Subject: JEOL JSM-6010LA InTouchScope 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="us-ascii" 10, 25 -- Content-Disposition: inline 10, 25 -- Content-Transfer-Encoding: 8bit 10, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5O7IkcE009947 ==============================End of - Headers==============================
If you are working above 10kV and at a max of 5,000X I see no point of moving to LaB6. LaB6 is brighter than tungsten and running at a lower temperature has less chromatic aberration. The problem with LaB6 is that you must have a very good vacuum or the possibility of a discharge could be catastrophic! A discharge may seed a crystal in what is meant to be a single crystal tip. Unfortunately over time this seed crystal may grow and eventually you have a double emitting source. Nice for fancy images but not for science! Thus great caution is required when running up the LaB6 filament and changing kV in order to prevent discharge occurring.
If you were always working below 5kV and looking at images I would suggest LaB6 as due to the higher brightness and lower aberrations this source will always better tungsten at these levels.
Enjoy!
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: 24 June 2011 00:39 To: protrain-at-emcourses.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Mathew_1980-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I'm new at SEM EDX. At my new place we have a jeol jsm 6460lv with capability for both LaB6 and tungsten filaments. Most of the EDX work done here was made using the W anode; however, nobody can really explain why and that is my question: -For SEM EDX at magnifications of ~ 5000x, what source is better LaB6 or W filament? Why?
We are delighted to bring to your attention the following workshop at the Center for Nanophase Materials Sciences: Advanced Scanning Probe Microscopies at the CNMS: Materials Structure and Function from Atomic to Micron Scales
September 21-22, 2011
Organizers: Arthur P. Baddorf (ORNL) Sergei V. Kalinin (ORNL)
Invited Lecturers: S. Jesse, A. Tselev, A.P. Li, P. Maksymovych, N. Balke, Z. Gai, M. Pan With participation of Asylum Research, Anasys, Bruker, NT-MDT, Omicron NanoTechnology USA, RHK, and SPECS
Development of nanoscience and nanotechnology requires the capability to image, manipulate, and control matter and energy on the nanometer, molecular, and ultimately, atomic levels. Scanning probe microscopy (SPM) techniques provide unparalleled access to the nanoscale world through structural, functional, and chemical imaging and manipulation on nanometer and atomic scales. Beyond imaging surface topography, SPMs have found an extremely broad range of applications for probing electronic, transport, optical, magnetic, mechanical, and electromechanical properties – often at the level of several tens of nanometers and below. Achieving full potential of SPM requires focused effort on developing novel SPM platforms and imaging modes, data interpretation routines, as well as capabilities for the in-situ sample preparation.
This workshop features presentations by CNMS staff members and SPM industry focused on technical capabilities developed and/or available at CNMS. These include:
• Atomic and vibrational imaging by low-temperature high magnetic field STM • Transport characterization by 4-probe STM/SEM • Scanning Electron Microscopy with Polarization Analysis • Piezoresponse Force Microscopy and Spectroscopy • Band Excitation SPMs for thermal, magnetic, and mechanical property mapping • Electrochemical Strain Microscopy of Li-ion and oxygen conductors • Microwave imaging and spectroscopy
This workshop will also feature presentation by leading SPM vendors devoted to the latest advances in SPM modes. Ultimately, we aim to build a network of advanced SPM practitioners to promote rapid dissemination of theoretical knowledge, experimental protocols, and novel technique development in these rapidly growing areas.
For more information and registration, please check regularly the CNMS web site at www.cnms.ornl.gov or contact the organizers at sergei2-at-ornl.gov or baddorfap-at-ornl.gov.
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 15, 25 -- From sergei2-at-ornl.gov Fri Jun 24 13:23:11 2011 15, 25 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OINB6n015168 15, 25 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 13:23:11 -0500 15, 25 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 15, 25 -- by emroute3.ornl.gov (PMDF V6.5-x6 #31890) 15, 25 -- with ESMTP id {0LNB007304EMNY-at-emroute3.ornl.gov} for 15, 25 -- microscopy-at-microscopy.com; Fri, 24 Jun 2011 14:23:11 -0400 (EDT) 15, 25 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 15, 25 -- (PMDF V6.5-x6 #31890) id {0LNB007014EMVZ-at-emroute3.ornl.gov} for 15, 25 -- microscopy-at-microscopy.com; Fri, 24 Jun 2011 14:23:10 -0400 (EDT) 15, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 15, 25 -- by emroute3.ornl.gov (PMDF V6.5-x6 #31890) 15, 25 -- with ESMTPS id {0LNB007804EMKM-at-emroute3.ornl.gov} for 15, 25 -- microscopy-at-microscopy.com; Fri, 24 Jun 2011 14:23:10 -0400 (EDT) 15, 25 -- Date: Fri, 24 Jun 2011 14:23:10 -0400 15, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 15, 25 -- Subject: Advanced SPM Workshop at CNMS 15, 25 -- To: microscopy-at-microscopy.com 15, 25 -- Message-id: {4E04D60E.6050800-at-ornl.gov} 15, 25 -- MIME-version: 1.0 15, 25 -- Content-type: text/plain; charset=windows-1252; format=flowed 15, 25 -- Content-transfer-encoding: 8BIT 15, 25 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.17) 15, 25 -- Gecko/20110414 Lightning/1.0b2 Thunderbird/3.1.10 ==============================End of - Headers==============================
Dear Group: has anyone had any luck in finding a system that users can log into - when using an instrument - this system would keep track of time used on the instrument and would help in creating invoices. Any recommendations would be greatly appreciated. Also this may help in keeping hackers out of your system. Thanks Barbara
==============================Original Headers============================== 1, 16 -- From maloneyb-at-fiu.edu Fri Jun 24 14:54:56 2011 1, 16 -- Received: from fiumailsmtp.fiu.edu (dithub02.ad.fiu.edu [131.94.72.23]) 1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OJsufg032555 1, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 14:54:56 -0500 1, 16 -- Received: from [131.94.220.250] (131.94.220.250) by fiumailsmtp.fiu.edu 1, 16 -- (192.168.251.14) with Microsoft SMTP Server (TLS) id 14.1.270.1; Fri, 24 Jun 1, 16 -- 2011 15:54:55 -0400 1, 16 -- Message-ID: {4E04EB8F.9090108-at-fiu.edu} 1, 16 -- Date: Fri, 24 Jun 2011 15:54:55 -0400 1, 16 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 16 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Thunderbird/3.1.11 1, 16 -- MIME-Version: 1.0 1, 16 -- To: {Microscopy-at-microscopy.com} 1, 16 -- Subject: accounting software and instrument usage 1, 16 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 1, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
One flaw in the concept of feeding "log in" and "log out" into an accounting system is that it assumes one uses all the time one has reserved. If a client books an instrument from 2 pm to 5 pm, but arrives and logs in at 3:15 and leaves at 4:15, they would be billed for 1 hr even though the reservation kept others from using the instrument for 3 hours. We are often not selling beam time as much as room time. We use the Calcium software http://www.brownbearsw.com/calcium/ program to schedule our core instruments. Any client can log in to the calendar on line to see when free time exists but they can't add or delete a reservation on their own. They are required to call core staff to book time. That prevents someone who is unqualified from reserving an instrument or a qualified user cancelling a reservation at the last minute and avoid being billed. The booking information can be exported out to an excel spread sheet for billing purposes. It is not fully automated but it works pretty well. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: maloneyb-at-fiu.edu [mailto:maloneyb-at-fiu.edu] Sent: Friday, June 24, 2011 2:55 PM To: Phillips, Thomas E.
Dear Group: has anyone had any luck in finding a system that users can log into - when using an instrument - this system would keep track of time used on the instrument and would help in creating invoices. Any recommendations would be greatly appreciated. Also this may help in keeping hackers out of your system. Thanks Barbara
==============================Original Headers============================== 1, 16 -- From maloneyb-at-fiu.edu Fri Jun 24 14:54:56 2011 1, 16 -- Received: from fiumailsmtp.fiu.edu (dithub02.ad.fiu.edu [131.94.72.23]) 1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OJsufg032555 1, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 14:54:56 -0500 1, 16 -- Received: from [131.94.220.250] (131.94.220.250) by fiumailsmtp.fiu.edu 1, 16 -- (192.168.251.14) with Microsoft SMTP Server (TLS) id 14.1.270.1; Fri, 24 Jun 1, 16 -- 2011 15:54:55 -0400 1, 16 -- Message-ID: {4E04EB8F.9090108-at-fiu.edu} 1, 16 -- Date: Fri, 24 Jun 2011 15:54:55 -0400 1, 16 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 16 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Thunderbird/3.1.11 1, 16 -- MIME-Version: 1.0 1, 16 -- To: {Microscopy-at-microscopy.com} 1, 16 -- Subject: accounting software and instrument usage 1, 16 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 1, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 12, 31 -- From PhillipsT-at-missouri.edu Fri Jun 24 15:05:26 2011 12, 31 -- Received: from mxnip01-missouri-out.um.umsystem.edu (mxnip01-missouri-out.um.umsystem.edu [209.106.229.53]) 12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OK5QEZ016347 12, 31 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 15:05:26 -0500 12, 31 -- X-IronPort-Anti-Spam-Filtered: true 12, 31 -- X-IronPort-Anti-Spam-Result: AggBAFntBE7Raua1/2dsb2JhbABNBhCYC48kd8E0iHkCgzeCdASHKZotVQ 12, 31 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) 12, 31 -- by mxnip01-mizzou-out.um.umsystem.edu with ESMTP; 24 Jun 2011 15:05:25 -0500 12, 31 -- Received: from um-email05.um.umsystem.edu ([169.254.1.116]) by 12, 31 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Fri, 24 Jun 2011 12, 31 -- 15:05:25 -0500 12, 31 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 12, 31 -- To: "maloneyb-at-fiu.edu" {maloneyb-at-fiu.edu} , 12, 31 -- "microscopy-at-microscopy.com" 12, 31 -- {microscopy-at-microscopy.com} 12, 31 -- Date: Fri, 24 Jun 2011 15:05:24 -0500 12, 31 -- Subject: RE: [Microscopy] accounting software and instrument usage 12, 31 -- Thread-Topic: [Microscopy] accounting software and instrument usage 12, 31 -- Thread-Index: AcwyqKt/QvFmWg61R6e6Gba6FruOOwAAEeGA 12, 31 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF267C0964F1-at-UM-EMAIL05.um.umsystem.edu} 12, 31 -- References: {201106241955.p5OJtRJq001040-at-ns.microscopy.com} 12, 31 -- In-Reply-To: {201106241955.p5OJtRJq001040-at-ns.microscopy.com} 12, 31 -- Accept-Language: en-US 12, 31 -- Content-Language: en-US 12, 31 -- X-MS-Has-Attach: 12, 31 -- X-MS-TNEF-Correlator: 12, 31 -- acceptlanguage: en-US 12, 31 -- Content-Type: text/plain; charset="us-ascii" 12, 31 -- MIME-Version: 1.0 12, 31 -- Content-Transfer-Encoding: 8bit 12, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5OK5QEZ016347 ==============================End of - Headers==============================
We use OCF for scheduling but haven't used the billing functionality of it yet. It is reservation versus logon/logout. As Tom Phillips commented, you probably want to think about capturing room time as it prevents others from accessing the equipment. http://bcf.arl.arizona.edu/
FOM will capture login/logout but you need to buy swipe card readers at each instrument (if I remember correctly). http://www.fomnetworks.com/FOM_features.html
Randy
-----Original Message----- X-from: maloneyb-at-fiu.edu [mailto:maloneyb-at-fiu.edu] Sent: Friday, June 24, 2011 3:03 PM To: Nessler, Randy A
Dear Group: has anyone had any luck in finding a system that users can log into - when using an instrument - this system would keep track of time used on the instrument and would help in creating invoices. Any recommendations would be greatly appreciated. Also this may help in keeping hackers out of your system. Thanks Barbara
==============================Original Headers============================== 1, 16 -- From maloneyb-at-fiu.edu Fri Jun 24 14:54:56 2011 1, 16 -- Received: from fiumailsmtp.fiu.edu (dithub02.ad.fiu.edu [131.94.72.23]) 1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OJsufg032555 1, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 14:54:56 -0500 1, 16 -- Received: from [131.94.220.250] (131.94.220.250) by fiumailsmtp.fiu.edu 1, 16 -- (192.168.251.14) with Microsoft SMTP Server (TLS) id 14.1.270.1; Fri, 24 Jun 1, 16 -- 2011 15:54:55 -0400 1, 16 -- Message-ID: {4E04EB8F.9090108-at-fiu.edu} 1, 16 -- Date: Fri, 24 Jun 2011 15:54:55 -0400 1, 16 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 16 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Thunderbird/3.1.11 1, 16 -- MIME-Version: 1.0 1, 16 -- To: {Microscopy-at-microscopy.com} 1, 16 -- Subject: accounting software and instrument usage 1, 16 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 1, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________
==============================Original Headers============================== 14, 31 -- From randy-nessler-at-uiowa.edu Fri Jun 24 15:21:02 2011 14, 31 -- Received: from HC-EDGE2.healthcare.uiowa.edu (hc-edge2.healthcare.uiowa.edu [129.255.126.23]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OKL2uK032633 14, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 15:21:02 -0500 14, 31 -- Received: from HC-HUB1.healthcare.uiowa.edu (129.255.112.194) by 14, 31 -- HC-EDGE2.healthcare.uiowa.edu (129.255.126.23) with Microsoft SMTP Server 14, 31 -- (TLS) id 14.1.289.1; Fri, 24 Jun 2011 15:20:58 -0500 14, 31 -- Received: from HC-MAILBOXC1-N5.healthcare.uiowa.edu ([169.254.5.3]) by 14, 31 -- HC-HUB1.healthcare.uiowa.edu ([129.255.112.194]) with mapi id 14.01.0289.001; 14, 31 -- Fri, 24 Jun 2011 15:21:01 -0500 14, 31 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 14, 31 -- To: "maloneyb-at-fiu.edu" {maloneyb-at-fiu.edu} , 14, 31 -- "Microscopy-at-microscopy.com" 14, 31 -- {Microscopy-at-microscopy.com} 14, 31 -- Subject: RE: [Microscopy] accounting software and instrument usage 14, 31 -- Thread-Topic: [Microscopy] accounting software and instrument usage 14, 31 -- Thread-Index: AQHMMqmpNbnA1uTvAUaiAjzW9xhcKZTM8Ttw 14, 31 -- Date: Fri, 24 Jun 2011 20:21:01 +0000 14, 31 -- Message-ID: {962DA0541BCBF346AAC391AE682C87A2048FA0E8-at-HC-MAILBOXC1-N5.healthcare.uiowa.edu} 14, 31 -- References: {201106242002.p5OK2Y3O014831-at-ns.microscopy.com} 14, 31 -- In-Reply-To: {201106242002.p5OK2Y3O014831-at-ns.microscopy.com} 14, 31 -- Accept-Language: en-US 14, 31 -- Content-Language: en-US 14, 31 -- X-MS-Has-Attach: 14, 31 -- X-MS-TNEF-Correlator: 14, 31 -- x-originating-ip: [172.26.0.1] 14, 31 -- x-my-disclaimer: ready 14, 31 -- Content-Type: text/plain; charset="us-ascii" 14, 31 -- MIME-Version: 1.0 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5OKL2uK032633 ==============================End of - Headers==============================
Claim: This email is sent on behalf of FOM Networks, Inc.
Dear Barbara and Randy, I believe the FOM system is the best solution that suits your needs. A typical use case of the system is as follows: 1) The user reserves a session via a standard webpage. There is no need to install any client-side software. 2) The user logs into the system and turns on a hardware access control box or a software access control to the instrument. This ensures the accurate login/logoff time. 3) The user logs off when he is done and the access control is turned off. 4) At the end of each month, the manager downloads fully customizable reports/invoices.
Please feel free to email me offline if you have more questions. Thanks, Shuyou
_________________ Shuyou Li, Ph.D. FOM Networks, Inc. www.fomnetworks.com 224-225-9168
-----Original Message----- X-from: randy-nessler-at-uiowa.edu [mailto:randy-nessler-at-uiowa.edu] Sent: Friday, June 24, 2011 3:23 PM To: syli-at-northwestern.edu
We use OCF for scheduling but haven't used the billing functionality of it yet. It is reservation versus logon/logout. As Tom Phillips commented, you probably want to think about capturing room time as it prevents others from accessing the equipment. http://bcf.arl.arizona.edu/
FOM will capture login/logout but you need to buy swipe card readers at each instrument (if I remember correctly). http://www.fomnetworks.com/FOM_features.html
Randy
-----Original Message----- X-from: maloneyb-at-fiu.edu [mailto:maloneyb-at-fiu.edu] Sent: Friday, June 24, 2011 3:03 PM To: Nessler, Randy A
Dear Group: has anyone had any luck in finding a system that users can log into - when using an instrument - this system would keep track of time used on the instrument and would help in creating invoices. Any recommendations would be greatly appreciated. Also this may help in keeping hackers out of your system. Thanks Barbara
==============================Original Headers============================== 1, 16 -- From maloneyb-at-fiu.edu Fri Jun 24 14:54:56 2011 1, 16 -- Received: from fiumailsmtp.fiu.edu (dithub02.ad.fiu.edu [131.94.72.23]) 1, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OJsufg032555 1, 16 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 14:54:56 -0500 1, 16 -- Received: from [131.94.220.250] (131.94.220.250) by fiumailsmtp.fiu.edu 1, 16 -- (192.168.251.14) with Microsoft SMTP Server (TLS) id 14.1.270.1; Fri, 24 Jun 1, 16 -- 2011 15:54:55 -0400 1, 16 -- Message-ID: {4E04EB8F.9090108-at-fiu.edu} 1, 16 -- Date: Fri, 24 Jun 2011 15:54:55 -0400 1, 16 -- From: Barbara Maloney {maloneyb-at-fiu.edu} 1, 16 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Thunderbird/3.1.11 1, 16 -- MIME-Version: 1.0 1, 16 -- To: {Microscopy-at-microscopy.com} 1, 16 -- Subject: accounting software and instrument usage 1, 16 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed 1, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________
==============================Original Headers============================== 14, 31 -- From randy-nessler-at-uiowa.edu Fri Jun 24 15:21:02 2011 14, 31 -- Received: from HC-EDGE2.healthcare.uiowa.edu (hc-edge2.healthcare.uiowa.edu [129.255.126.23]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5OKL2uK032633 14, 31 -- for {Microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 15:21:02 -0500 14, 31 -- Received: from HC-HUB1.healthcare.uiowa.edu (129.255.112.194) by 14, 31 -- HC-EDGE2.healthcare.uiowa.edu (129.255.126.23) with Microsoft SMTP Server 14, 31 -- (TLS) id 14.1.289.1; Fri, 24 Jun 2011 15:20:58 -0500 14, 31 -- Received: from HC-MAILBOXC1-N5.healthcare.uiowa.edu ([169.254.5.3]) by 14, 31 -- HC-HUB1.healthcare.uiowa.edu ([129.255.112.194]) with mapi id 14.01.0289.001; 14, 31 -- Fri, 24 Jun 2011 15:21:01 -0500 14, 31 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 14, 31 -- To: "maloneyb-at-fiu.edu" {maloneyb-at-fiu.edu} , 14, 31 -- "Microscopy-at-microscopy.com" 14, 31 -- {Microscopy-at-microscopy.com} 14, 31 -- Subject: RE: [Microscopy] accounting software and instrument usage 14, 31 -- Thread-Topic: [Microscopy] accounting software and instrument usage 14, 31 -- Thread-Index: AQHMMqmpNbnA1uTvAUaiAjzW9xhcKZTM8Ttw 14, 31 -- Date: Fri, 24 Jun 2011 20:21:01 +0000 14, 31 -- Message-ID: {962DA0541BCBF346AAC391AE682C87A2048FA0E8-at-HC-MAILBOXC1-N5.healthcare.uiowa.e du} 14, 31 -- References: {201106242002.p5OK2Y3O014831-at-ns.microscopy.com} 14, 31 -- In-Reply-To: {201106242002.p5OK2Y3O014831-at-ns.microscopy.com} 14, 31 -- Accept-Language: en-US 14, 31 -- Content-Language: en-US 14, 31 -- X-MS-Has-Attach: 14, 31 -- X-MS-TNEF-Correlator: 14, 31 -- x-originating-ip: [172.26.0.1] 14, 31 -- x-my-disclaimer: ready 14, 31 -- Content-Type: text/plain; charset="us-ascii" 14, 31 -- MIME-Version: 1.0 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5OKL2uK032633 ==============================End of - Headers==============================
==============================Original Headers============================== 27, 23 -- From syli-at-northwestern.edu Fri Jun 24 20:47:10 2011 27, 23 -- Received: from mailp8.ci.northwestern.edu (casbah.it.northwestern.edu [129.105.16.98]) 27, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5P1lADJ019862 27, 23 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 20:47:10 -0500 27, 23 -- Received: from ShuyouPC (prep.ms.northwestern.edu [129.105.37.239]) 27, 23 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 27, 23 -- (No client certificate requested) 27, 23 -- by mailp8.ci.northwestern.edu (Postfix) with ESMTP id 5862376E15 27, 23 -- for {microscopy-at-microscopy.com} ; Fri, 24 Jun 2011 20:47:10 -0500 (CDT) 27, 23 -- From: "Shuyou Li" {syli-at-northwestern.edu} 27, 23 -- To: {microscopy-at-microscopy.com} 27, 23 -- References: {201106242023.p5OKNC5Q005371-at-ns.microscopy.com} 27, 23 -- In-Reply-To: {201106242023.p5OKNC5Q005371-at-ns.microscopy.com} 27, 23 -- Subject: RE: [Microscopy] RE: accounting software and instrument usage 27, 23 -- Date: Fri, 24 Jun 2011 20:47:15 -0500 27, 23 -- Message-ID: {061001cc32d9$cf41cdb0$6dc56910$-at-northwestern.edu} 27, 23 -- MIME-Version: 1.0 27, 23 -- Content-Type: text/plain; 27, 23 -- charset="us-ascii" 27, 23 -- Content-Transfer-Encoding: 7bit 27, 23 -- X-Mailer: Microsoft Outlook 14.0 27, 23 -- Thread-Index: AQHJf3m8TlWhIQkE0TPBM1qY1pucEZTSmCJQ 27, 23 -- Content-Language: en-us ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both luo-at-tamu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: luo-at-tamu.edu Name: Zhiping Luo
Organization: Texas A&M University
Title-Subject: [Filtered] RE: Tem EDX
Message: Hi Debby,
I agree with Henk that you should not see the elements from the p-p if the detector geometry is optimized. You need to request for service to figure out where is the problem.
Normally the possible spurious source is the grids if you use, as well as the specimen holder when it is translated far way from its zero position.
Sincerely,
Zhiping Luo
} Date: Thu, 23 Jun 2011 16:28:09 -0500 } To: zhiping_luo-at-hotmail.com } From: colijn.1-at-osu.edu } Subject: [Microscopy] Tem EDX } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Debby, } } While the Fe, Co etc are undoubtedly from the pole-piece, I wonder why } you see such a significant contribution to the spectrum. As long as the } objective aperture is out, even the scattered electrons should go down } the bore of the pole-piece. Brehmstrahlung from the condenser apertures } may have some effect, but it should be small. } } Analytical scopes have improved dramatically over the years and I } thought stray signal was pretty much a thing of the past. Pretty much } every microscope I've used in the past 25 years has almost no x-rays } from the p-p. Do you have an unusual scope? (You can reply off-line if } you don't wish to identify the scope publicly.) In my experience, the } EDX collimators on TEM detectors don't shield very much, so the } improvements in stray signal have been due to changes in the column itself. } } Cheers, } Henk } } } At 6/22/2011 8:32 PM, dsherman-at-purdue.edu wrote: } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Thanks to all for the quick response. Consensus is that the reviewer is } } incorrect as many scopes do see Fe and Co in EDX spectra from the polepiece. } } The amount may depend on the type of microscope, detector, etc but } } apparently many instruments show this. } } } } I didn't mention this in my first E-mail but I regularly take a background } } spectrum and they invariably show Fe and Co in our instrument. It is } } difficult to do a background subtraction and remove all of it. Presumably } } this is because the sample does deflect many more electrons than just the } } support film resulting in more X-ray production from other areas within the } } objective lens. } } } } We also have a cryo blade that is made of copper and also the connection } } from the external nitrogen dewar to the decontaminator is a copper braid. } } Thus, even when using a low background (Beryllium) holder and nickel grid we } } often get a small amount of Cu. This may not show up with a SiLi detector } } but we get so many counts with the SDD that this may translate into higher } } background peaks for some of these elements. } } } } Debby } } } } } } On 6/22/11 6:15 PM, "Debby Sherman" {dsherman-at-purdue.edu} wrote: } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } } } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi all, } } } } } } We have a SDD EDX system on our TEM and routinely get Co, Cu, and Fe in the } } } spectra. I understood that these elements are from the polepiece and other } } } components in the sample area. } } } } } } When a student submitted a manuscript with these elements in the spectrum, } } } along with the other elements of interest (IN, Sb), a reviewer said that } } } the TEM instruments are strictly restricted from using the magnetic } } } impurities/parts, so there must be other sources for iron. } } } } } } Comments? How would you respond to this reviewer. } } } } } } Debby } } } } } } --- } } } Debby Sherman, Director Phone: 765-494-6666 } } } Life Science Microscopy Facility FAX: 765-494-5896 } } } Purdue University E-mail:dsherman-at-purdue.edu } } } S-052 Whistler Building } } } 170 S. University Street } } } West Lafayette, IN 47907 } } } http://www.ag.purdue.edu/facilities/microscopy } } }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both cmazareanu-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: cmazareanu-at-yahoo.com Name: Constantin
Title-Subject: [Filtered] wanted to buy DRV-2000
Message: Hi, I am looking to buy a pc isa board DRV-2000 and its additional software. It can be good or defective. Thank you
Hi all, I need advice on dismantling a Reichert 2800 cryostat. I cannot remove the handle. I've tried an allen wrench but I can't get it to budge. Is there a magic alignment? thanks for any help, Beth
==============================Original Headers============================== 2, 19 -- From beth-at-plantbio.uga.edu Mon Jun 27 16:16:23 2011 2, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 2, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5RLGMDH015843 2, 19 -- for {microscopy-at-microscopy.com} ; Mon, 27 Jun 2011 16:16:23 -0500 2, 19 -- Received: from [128.192.26.69] ([128.192.26.69]) 2, 19 -- (authenticated user beth-at-plantbio.uga.edu) 2, 19 -- by dogwood.plantbio.uga.edu 2, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 2, 19 -- for microscopy-at-microscopy.com; 2, 19 -- Mon, 27 Jun 2011 17:16:21 -0400 2, 19 -- Message-Id: {17ADC648-84FC-4372-94BE-38240F701D17-at-plantbio.uga.edu} 2, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 2, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 2, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 2, 19 -- Content-Transfer-Encoding: 7bit 2, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 2, 19 -- Subject: removing the handle from a Reichert 2800 cryostat 2, 19 -- Date: Mon, 27 Jun 2011 17:16:20 -0400 2, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
1. How to get coordinates of the most intense pixels
I want to get coordinates of the most intense pixels in an area on STEM image. Manually I can find them on Digital Micrograph and get the coordinates, but I am looking for a more convenient method. If there is a software for such purpose, please advise.
Because of the background noise, the most intense pixels are not always the atom positions. If there is a software that handles the background noise problem and finds true “most intense pixels”, please advise.
2. How to get total pixel number in a ROI on Digital Micrograph
I want to know how to get the total pixel number in a ROI.
Thank you,
Hiromi
UW-Madison
==============================Original Headers============================== 30, 35 -- From hkonishi-at-wisc.edu Tue Jun 28 17:02:25 2011 30, 35 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 30, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5SM2P3v027738 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Jun 2011 17:02:25 -0500 30, 35 -- MIME-version: 1.0 30, 35 -- Content-disposition: inline 30, 35 -- Content-type: text/plain; charset=windows-1252 30, 35 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 30, 35 -- smtpauth1.wiscmail.wisc.edu 30, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 30, 35 -- id {0LNI00300T81Z300-at-smtpauth1.wiscmail.wisc.edu} for 30, 35 -- Microscopy-at-microscopy.com; Tue, 28 Jun 2011 17:02:25 -0500 (CDT) 30, 35 -- Received: from wisc.edu (wmcnv1pvt.pri.doit.wisc.edu [144.92.8.208]) 30, 35 -- by smtpauth1.wiscmail.wisc.edu 30, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 30, 35 -- with ESMTPA id {0LNI00KTDT80LZ20-at-smtpauth1.wiscmail.wisc.edu} for 30, 35 -- Microscopy-at-microscopy.com; Tue, 28 Jun 2011 17:02:24 -0500 (CDT) 30, 35 -- Received: from [144.92.197.149] (Forwarded-For: 144.92.207.51) 30, 35 -- by wmcnv1.doit.wisc.edu (mshttpd); Tue, 28 Jun 2011 17:02:24 -0500 30, 35 -- Date: Tue, 28 Jun 2011 17:02:24 -0500 30, 35 -- From: Hiromi Konishi {hkonishi-at-wisc.edu} 30, 35 -- Subject: Software to find the most intense pixels 30, 35 -- To: Microscopy-at-microscopy.com 30, 35 -- Message-id: {7580c1d08facb.4e0a0920-at-wiscmail.wisc.edu} 30, 35 -- X-Mailer: Oracle Communications Messenger Express 7u4-19.01 64bit (built Sep 7 30, 35 -- 2010) 30, 35 -- Content-language: en 30, 35 -- X-Accept-Language: en 30, 35 -- Priority: normal 30, 35 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.8.208 30, 35 -- X-Spam-PmxInfo: Server=avs-13, Version=5.6.1.2065439, 30, 35 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.6.28.215415, 30, 35 -- SenderIP=144.92.8.208 30, 35 -- Content-Transfer-Encoding: 8bit 30, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5SM2P3v027738 ==============================End of - Headers==============================
Have a look at ImageJ. This is a free image analysis program that has a lot of usefull features and plugins. Total number of pixels in ROI is generated automatically when you make/select a ROI. Coordinate of most intense pixel(s) can be found as well, by performing a measurement. If this specific option is not present it should be rather straightforward to write your own macro that will give you the x,y coordinate.
Kind regards
dr. Peter Zoon Forensic Scientist
.................................................................................. Netherlands Forensisc Institute Microtrace Department Laan van Ypenburg 6 | 2497 GB | The Hague Postbus 24044 | 2490 AA | The Hague
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
I have two questions.
1. How to get coordinates of the most intense pixels
I want to get coordinates of the most intense pixels in an area on STEM image. Manually I can find them on Digital Micrograph and get the coordinates, but I am looking for a more convenient method. If there is a software for such purpose, please advise.
Because of the background noise, the most intense pixels are not always the atom positions. If there is a software that handles the background noise problem and finds true "most intense pixels", please advise.
2. How to get total pixel number in a ROI on Digital Micrograph
I want to know how to get the total pixel number in a ROI.
Thank you,
Hiromi
UW-Madison
==============================Original Headers============================== 30, 35 -- From hkonishi-at-wisc.edu Tue Jun 28 17:02:25 2011 30, 35 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 30, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5SM2P3v027738 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Jun 2011 17:02:25 -0500 30, 35 -- MIME-version: 1.0 30, 35 -- Content-disposition: inline 30, 35 -- Content-type: text/plain; charset=windows-1252 30, 35 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 30, 35 -- smtpauth1.wiscmail.wisc.edu 30, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 30, 35 -- id {0LNI00300T81Z300-at-smtpauth1.wiscmail.wisc.edu} for 30, 35 -- Microscopy-at-microscopy.com; Tue, 28 Jun 2011 17:02:25 -0500 (CDT) 30, 35 -- Received: from wisc.edu (wmcnv1pvt.pri.doit.wisc.edu [144.92.8.208]) 30, 35 -- by smtpauth1.wiscmail.wisc.edu 30, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 30, 35 -- with ESMTPA id {0LNI00KTDT80LZ20-at-smtpauth1.wiscmail.wisc.edu} for 30, 35 -- Microscopy-at-microscopy.com; Tue, 28 Jun 2011 17:02:24 -0500 (CDT) 30, 35 -- Received: from [144.92.197.149] (Forwarded-For: 144.92.207.51) 30, 35 -- by wmcnv1.doit.wisc.edu (mshttpd); Tue, 28 Jun 2011 17:02:24 -0500 30, 35 -- Date: Tue, 28 Jun 2011 17:02:24 -0500 30, 35 -- From: Hiromi Konishi {hkonishi-at-wisc.edu} 30, 35 -- Subject: Software to find the most intense pixels 30, 35 -- To: Microscopy-at-microscopy.com 30, 35 -- Message-id: {7580c1d08facb.4e0a0920-at-wiscmail.wisc.edu} 30, 35 -- X-Mailer: Oracle Communications Messenger Express 7u4-19.01 64bit (built Sep 7 30, 35 -- 2010) 30, 35 -- Content-language: en 30, 35 -- X-Accept-Language: en 30, 35 -- Priority: normal 30, 35 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.8.208 30, 35 -- X-Spam-PmxInfo: Server=avs-13, Version=5.6.1.2065439, 30, 35 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.6.28.215415, 30, 35 -- SenderIP=144.92.8.208 30, 35 -- Content-Transfer-Encoding: 8bit 30, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5SM2P3v027738 ==============================End of - Headers==============================
-------------------------------------------------------------------- Dit bericht kan informatie bevatten die niet voor u is bestemd. Indien u niet de geadresseerde bent of dit bericht abusievelijk aan u is toegezonden, wordt u verzocht dat aan de afzender te melden en het bericht te verwijderen. De Staat aanvaardt geen aansprakelijkheid voor schade, van welke aard ook, die verband houdt met risico's verbonden aan het elektronisch verzenden van berichten.
This message may contain information that is not intended for you. If you are not the addressee or if this message was sent to you by mistake, you are requested to inform the sender and delete the message. The State accepts no liability for damage of any kind resulting from the risks inherent in the electronic transmission of messages.
Nederlands Forensisch Instituut Ministerie van Veiligheid en Justitie
==============================Original Headers============================== 42, 30 -- From p.zoon-at-nfi.minjus.nl Wed Jun 29 01:30:33 2011 42, 30 -- Received: from minjus.nl (smtp1.minjus.nl [159.46.2.165]) 42, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5T6UWmW023194 42, 30 -- for {microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 01:30:32 -0500 42, 30 -- Received: from ([10.48.129.102]) 42, 30 -- by smtp1.minjus.nl with ESMTP with TLS id 1Z84LF1.520580290; 42, 30 -- Wed, 29 Jun 2011 08:30:25 +0200 42, 30 -- Received: from ([10.63.0.72]) 42, 30 -- by smtp12.minjus.nl with ESMTP id D5DC7G1.27421799; 42, 30 -- Wed, 29 Jun 2011 08:30:23 +0200 42, 30 -- Received: from nfi-vmexch-01.nfi.minjus.nl ([10.1.200.65]) by NFI-IIS-01.nfi.minjus.nl with Microsoft SMTPSVC(6.0.3790.3959); Wed, 29 Jun 2011 08:30:23 +0200 42, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4133 42, 30 -- Content-Class: urn:content-classes:message 42, 30 -- MIME-Version: 1.0 42, 30 -- Content-Type: text/plain; 42, 30 -- charset="iso-8859-1" 42, 30 -- Subject: RE: [Microscopy] Software to find the most intense pixels 42, 30 -- Importance: normal 42, 30 -- Priority: normal 42, 30 -- Date: Wed, 29 Jun 2011 08:30:23 +0200 42, 30 -- Message-ID: {99282C97DBCD874BBBD13938A9E5C9C705436014-at-nfi-vmexch-01.nfi.minjus.nl} 42, 30 -- X-MS-Has-Attach: 42, 30 -- X-MS-TNEF-Correlator: 42, 30 -- Thread-Topic: [Microscopy] Software to find the most intense pixels 42, 30 -- thread-index: Acw14HVhx1H5BV3MS7quHfqvYn486gARMTuw 42, 30 -- From: "Zoon, Peter" {p.zoon-at-nfi.minjus.nl} 42, 30 -- To: {Microscopy-at-microscopy.com} 42, 30 -- X-OriginalArrivalTime: 29 Jun 2011 06:30:23.0533 (UTC) FILETIME=[06A97DD0:01CC3626] 42, 30 -- Content-Transfer-Encoding: 8bit 42, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5T6UWmW023194 ==============================End of - Headers==============================
Hi Hiromi, after reading your question again, it seems that you are not simply looking for a pixel value. You mention STEM and atom positions, so I suppose that you rather want to locate the atom from noisy data. Is that correct?
If so, I think you will have to do some modeling. I don't know what intensity distribution you can expect from a single atom (or a column of atoms in a crystal) from STEM, but let's assume it could be something like a Gaussian distribution, where the maximum shows the most likely position of the atom. In that case you would have to set an ROI around the area of the atom, and fit a 2-D Gaussian to the data. This can also give you error values for the position of the maximum. If you have a crystal, then you can probably define a grid, and run your algorithm automatically on any field in the grid.
Unfortunately I can't help you with DM, but I am sure it has capabilities to do this.
Mike
--- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: hkonishi-at-wisc.edu [mailto:hkonishi-at-wisc.edu] Sent: Tuesday, June 28, 2011 4:13 PM To: mike.bode-at-resaltatech.com
I have two questions.
1. How to get coordinates of the most intense pixels
I want to get coordinates of the most intense pixels in an area on STEM image. Manually I can find them on Digital Micrograph and get the coordinates, but I am looking for a more convenient method. If there is a software for such purpose, please advise.
Because of the background noise, the most intense pixels are not always the atom positions. If there is a software that handles the background noise problem and finds true "most intense pixels", please advise.
2. How to get total pixel number in a ROI on Digital Micrograph
I want to know how to get the total pixel number in a ROI.
Thank you,
Hiromi
UW-Madison
==============================Original Headers============================== 30, 35 -- From hkonishi-at-wisc.edu Tue Jun 28 17:02:25 2011 30, 35 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 30, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5SM2P3v027738 30, 35 -- for {Microscopy-at-microscopy.com} ; Tue, 28 Jun 2011 17:02:25 -0500 30, 35 -- MIME-version: 1.0 30, 35 -- Content-disposition: inline 30, 35 -- Content-type: text/plain; charset=windows-1252 30, 35 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 30, 35 -- smtpauth1.wiscmail.wisc.edu 30, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 30, 35 -- id {0LNI00300T81Z300-at-smtpauth1.wiscmail.wisc.edu} for 30, 35 -- Microscopy-at-microscopy.com; Tue, 28 Jun 2011 17:02:25 -0500 (CDT) 30, 35 -- Received: from wisc.edu (wmcnv1pvt.pri.doit.wisc.edu [144.92.8.208]) 30, 35 -- by smtpauth1.wiscmail.wisc.edu 30, 35 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 30, 35 -- with ESMTPA id {0LNI00KTDT80LZ20-at-smtpauth1.wiscmail.wisc.edu} for 30, 35 -- Microscopy-at-microscopy.com; Tue, 28 Jun 2011 17:02:24 -0500 (CDT) 30, 35 -- Received: from [144.92.197.149] (Forwarded-For: 144.92.207.51) 30, 35 -- by wmcnv1.doit.wisc.edu (mshttpd); Tue, 28 Jun 2011 17:02:24 -0500 30, 35 -- Date: Tue, 28 Jun 2011 17:02:24 -0500 30, 35 -- From: Hiromi Konishi {hkonishi-at-wisc.edu} 30, 35 -- Subject: Software to find the most intense pixels 30, 35 -- To: Microscopy-at-microscopy.com 30, 35 -- Message-id: {7580c1d08facb.4e0a0920-at-wiscmail.wisc.edu} 30, 35 -- X-Mailer: Oracle Communications Messenger Express 7u4-19.01 64bit (built Sep 7 30, 35 -- 2010) 30, 35 -- Content-language: en 30, 35 -- X-Accept-Language: en 30, 35 -- Priority: normal 30, 35 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=144.92.8.208 30, 35 -- X-Spam-PmxInfo: Server=avs-13, Version=5.6.1.2065439, 30, 35 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.6.28.215415, 30, 35 -- SenderIP=144.92.8.208 30, 35 -- Content-Transfer-Encoding: 8bit 30, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5SM2P3v027738 ==============================End of - Headers==============================
==============================Original Headers============================== 49, 31 -- From mike.bode-at-resaltatech.com Wed Jun 29 10:07:59 2011 49, 31 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 49, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5TF7xvK015958 49, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 10:07:59 -0500 49, 31 -- Received: from c-174-51-0-206.hsd1.co.comcast.net ([174.51.0.206]:17185 helo=resdtpden01) 49, 31 -- by web38.justhost.com with esmtpa (Exim 4.69) 49, 31 -- (envelope-from {mike.bode-at-resaltatech.com} ) 49, 31 -- id 1QbwN0-0002r2-3j; Wed, 29 Jun 2011 10:08:02 -0500 49, 31 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 49, 31 -- To: {Microscopy-at-microscopy.com} 49, 31 -- Cc: {hkonishi-at-wisc.edu} 49, 31 -- References: {201106282213.p5SMDEBr008799-at-ns.microscopy.com} 49, 31 -- In-Reply-To: {201106282213.p5SMDEBr008799-at-ns.microscopy.com} 49, 31 -- Subject: RE: [Microscopy] Software to find the most intense pixels 49, 31 -- Date: Wed, 29 Jun 2011 09:07:50 -0600 49, 31 -- Message-ID: {001501cc366e$51db49d0$f591dd70$-at-bode-at-resaltatech.com} 49, 31 -- MIME-Version: 1.0 49, 31 -- Content-Type: text/plain; 49, 31 -- charset="us-ascii" 49, 31 -- Content-Transfer-Encoding: 7bit 49, 31 -- X-Mailer: Microsoft Office Outlook 12.0 49, 31 -- Thread-Index: Acw14JZz3909vgTYQMyx8IKZfywlNQAjAuvg 49, 31 -- Content-Language: en-us 49, 31 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 49, 31 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 49, 31 -- X-AntiAbuse: Original Domain - microscopy.com 49, 31 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 49, 31 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 49, 31 -- X-Source: 49, 31 -- X-Source-Args: 49, 31 -- X-Source-Dir: ==============================End of - Headers==============================
A researcher wants to study the uptake of 200 nm polystyrene beads using TEM of sectioned materials. I believe the beads will be dissolved in any solvent we use (including ethanol). I suggested other nanoparticles, but the researcher believes PS would be best for the work.
I was hoping to use ethanol with the epoxy resin (to avoid more powerful organic solvents like acetone and polypropylene) but the manufacturer of the beads said any organic solvent would dissolve the beads.
Anyone have experience using PS beads and embedding?
Thanks.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 18 -- From bozzola-at-siu.edu Wed Jun 29 12:30:49 2011 6, 18 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5THUn8r001958 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 12:30:49 -0500 6, 18 -- Received: by iwn8 with SMTP id 8so1608971iwn.0 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 10:30:48 -0700 (PDT) 6, 18 -- MIME-Version: 1.0 6, 18 -- Received: by 10.42.195.207 with SMTP id ed15mr1173159icb.61.1309368648602; 6, 18 -- Wed, 29 Jun 2011 10:30:48 -0700 (PDT) 6, 18 -- Received: by 10.42.165.71 with HTTP; Wed, 29 Jun 2011 10:30:48 -0700 (PDT) 6, 18 -- Date: Wed, 29 Jun 2011 12:30:48 -0500 6, 18 -- Message-ID: {BANLkTi=_C_eXtcZwOGjytzhsNQ+P=xZv_A-at-mail.gmail.com} 6, 18 -- Subject: TEM stability of polystyrene beads 6, 18 -- From: John Bozzola {bozzola-at-siu.edu} 6, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 18 -- Content-Type: text/plain; charset=UTF-8 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5THUn8r001958 ==============================End of - Headers==============================
You might want to remember that some of the Epon substitutes will also dissolve PS. I have used LX-112 from Ladd and it works well with PS. I assume that there are others that do also but EMbed-812 will not work with it for it melted my small polystyrene petri dishes.
I have no financial ties to the above products but simply use both.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
On Jun 29, 2011, at 1:39 PM, bozzola-at-siu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } A researcher wants to study the uptake of 200 nm polystyrene beads } using TEM of sectioned materials. I believe the beads will be } dissolved in any solvent we use (including ethanol). I suggested other } nanoparticles, but the researcher believes PS would be best for the } work. } } I was hoping to use ethanol with the epoxy resin (to avoid more } powerful organic solvents like acetone and polypropylene) but the } manufacturer of the beads said any organic solvent would dissolve the } beads. } } Anyone have experience using PS beads and embedding? } } Thanks. } } -- } John J. Bozzola, Ph.D., Professor & Director of IMAGE } Integrated Microscopy & Graphics Expertise } Southern Illinois University } 750 Communications Drive } Carbondale, ILÂ 62901 } Phone: 618-453-3730 } } } ==============================Original Headers============================== } 6, 18 -- From bozzola-at-siu.edu Wed Jun 29 12:30:49 2011 } 6, 18 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) } 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5THUn8r001958 } 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 12:30:49 -0500 } 6, 18 -- Received: by iwn8 with SMTP id 8so1608971iwn.0 } 6, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 10:30:48 -0700 (PDT) } 6, 18 -- MIME-Version: 1.0 } 6, 18 -- Received: by 10.42.195.207 with SMTP id ed15mr1173159icb.61.1309368648602; } 6, 18 -- Wed, 29 Jun 2011 10:30:48 -0700 (PDT) } 6, 18 -- Received: by 10.42.165.71 with HTTP; Wed, 29 Jun 2011 10:30:48 -0700 (PDT) } 6, 18 -- Date: Wed, 29 Jun 2011 12:30:48 -0500 } 6, 18 -- Message-ID: {BANLkTi=_C_eXtcZwOGjytzhsNQ+P=xZv_A-at-mail.gmail.com} } 6, 18 -- Subject: TEM stability of polystyrene beads } 6, 18 -- From: John Bozzola {bozzola-at-siu.edu} } 6, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} } 6, 18 -- Content-Type: text/plain; charset=UTF-8 } 6, 18 -- Content-Transfer-Encoding: 8bit } 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5THUn8r001958 } ==============================End of - Headers==============================
Michal Jarnik NICHD, CBMP Bldg 18T, Rm. 101 Tel. 301-594-3944
==============================Original Headers============================== 8, 25 -- From jarnikm-at-mail.nih.gov Wed Jun 29 14:54:40 2011 8, 25 -- Received: from nihrelayxway6.hub.nih.gov (nihrelayxway6.hub.nih.gov [128.231.90.100]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5TJseLI004466 8, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 29 Jun 2011 14:54:40 -0500 8, 25 -- X-IronPortListener: NIH_Relay 8, 25 -- X-SBRS: None 8, 25 -- X-IronPort-AV: E=Sophos;i="4.65,445,1304308800"; 8, 25 -- d="scan'208";a="73153033" 8, 25 -- Received: from mailfwd.nih.gov ([128.231.90.107]) 8, 25 -- by nihrelayxway6.hub.nih.gov with ESMTP; 29 Jun 2011 15:54:40 -0400 8, 25 -- Received: from [156.40.161.206] (156.40.161.206) by mailfwd.nih.gov 8, 25 -- (128.231.90.107) with Microsoft SMTP Server id 14.1.255.0; Wed, 29 Jun 2011 8, 25 -- 15:54:39 -0400 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 (Apple Message framework v1082) 8, 25 -- Subject: Re: [Microscopy] TEM stability of polystyrene beads 8, 25 -- From: Michal Jarnik {jarnikm-at-mail.nih.gov} 8, 25 -- In-Reply-To: {201106291739.p5THdg4m017672-at-ns.microscopy.com} 8, 25 -- Date: Wed, 29 Jun 2011 15:54:39 -0400 8, 25 -- Message-ID: {9896C283-8FB7-418E-BBE8-D1DF54B2B6EE-at-mail.nih.gov} 8, 25 -- References: {201106291739.p5THdg4m017672-at-ns.microscopy.com} 8, 25 -- To: {Microscopy-at-microscopy.com} 8, 25 -- X-Mailer: Apple Mail (2.1082) 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5TJseLI004466 ==============================End of - Headers==============================
I can confirm that polystyrene beads will dissolve. The research specimens that we processed using Acetone and Procure 812 resin demonstrated this. However, the loss of the beads (FLUORESBRITE PLAIN YG 2.00 MICRON MICROSPHERES) was not a concern with this project as the fluorescent yellow of the beads worked well to demonstrate the target area for dissection; and then the unmistakable uniformly round spaces in the infiltrating inflammatory cells was clearly visible in the semi-thin resin sections. Researcher was happy. As always it depends on what the researcher is seeking to demonstrate.
(No commercial associations) Regards Naomi
Naomi McCallum Supervising Scientist, EM Unit, Anatomical Pathology Pathology Queensland
} } } {connellyps-at-nhlbi.nih.gov} 6/30/2011 5:47 am } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
John,
You might want to remember that some of the Epon substitutes will also dissolve PS. I have used LX-112 from Ladd and it works well with PS. I assume that there are others that do also but EMbed-812 will not work with it for it melted my small polystyrene petri dishes.
I have no financial ties to the above products but simply use both.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
While there is no doubt that acetone dissolves plastics, I would be surprised if ethanol would do any harm to them. I would recommend bypassing the acetone step and directly embedding in ethanol/resin mixture after dehydration.
Just be sure to leave no trace of ethanol in the resin.
In any case it is quite straightforward to test the resistance of the beads to ethanol or anything else: leave them in ethanol overnight and check by SEM.
Best regards,
Stephane
----- Original Message ---- X-from: "naomi_mccallum-at-health.qld.gov.au" {naomi_mccallum-at-health.qld.gov.au} To: nizets2-at-yahoo.com Sent: Thu, June 30, 2011 12:35:23 AM
Good Morning
I can confirm that polystyrene beads will dissolve. The research specimens that we processed using Acetone and Procure 812 resin demonstrated this. However, the loss of the beads (FLUORESBRITE PLAIN YG 2.00 MICRON MICROSPHERES) was not a concern with this project as the fluorescent yellow of the beads worked well to demonstrate the target area for dissection; and then the unmistakable uniformly round spaces in the infiltrating inflammatory cells was clearly visible in the semi-thin resin sections. Researcher was happy. As always it depends on what the researcher is seeking to demonstrate.
(No commercial associations) Regards Naomi
Naomi McCallum Supervising Scientist, EM Unit, Anatomical Pathology Pathology Queensland
} } } {connellyps-at-nhlbi.nih.gov} 6/30/2011 5:47 am } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
John,
You might want to remember that some of the Epon substitutes will also dissolve PS. I have used LX-112 from Ladd and it works well with PS. I assume that there are others that do also but EMbed-812 will not work with it for it melted my small polystyrene petri dishes.
I have no financial ties to the above products but simply use both.
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
This is clearly a Zeiss AxioImager scope. Ah well, I guess we Canadians have a monarch of another country on change and other bills, why not a German scope on the $100.
JME
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
I am in a big (read urgent) need of a plug-in for ImageJ (or any other image processing software) that can help me quantify synaptic vesicles. I need to count vesicles and in the same time get one of these parameters: diameter or radius or area of the same vesicle. Automatic processing of images involving thresholding does not recognize many of the vesicles, so I’d like to have something like a semi-automatic process: I define where the vesicle is (e.g. by clicking in the centre), and the software does the rest. Does anybody know if there is any software/plug-in/macro for ImageJ that can help me? Or any other software (preferentially free)?
I am speaking of quantifying hundreds of vesicles in tens of end-plates...
Thanks in advance for the help.
Warmest regards at 42ºC from Seville,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
If you wish to test what solvents dissolve polystyrene try a low cost styrofoam cup. It dissolves is seconds if you try to fill it with acetone--tried it in chem lab decades ago, the lesson sticks. Since many alcoholic beverages are served in #6 PS cups, alcohol should be fine.
Have a great day! Roseann
Roseann Csencsits, PhD Supervising Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720
==============================Original Headers============================== 4, 29 -- From rcsencsits-at-lbl.gov Thu Jun 30 12:26:48 2011 4, 29 -- Received: from ironport3.lbl.gov (ironport3.lbl.gov [128.3.41.25]) 4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5UHQmuM006918 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jun 2011 12:26:48 -0500 4, 29 -- X-Ironport-SBRS: 3.8 4, 29 -- X-IronPort-Anti-Spam-Filtered: true 4, 29 -- X-IronPort-Anti-Spam-Result: AmUDAImxDE7RVda0imdsb2JhbABSmGaOaAgUAQEBCgkNBxIGIakhglCcTYYxBIdAlws8g3Y 4, 29 -- X-IronPort-AV: E=Sophos;i="4.65,453,1304319600"; 4, 29 -- d="scan'208";a="45443867" 4, 29 -- Received: from mail-iw0-f180.google.com ([209.85.214.180]) 4, 29 -- by ironport3.lbl.gov with ESMTP; 30 Jun 2011 10:26:47 -0700 4, 29 -- Received: by mail-iw0-f180.google.com with SMTP id 9so3531636iwn.39 4, 29 -- for {Microscopy-at-microscopy.com} ; Thu, 30 Jun 2011 10:26:47 -0700 (PDT) 4, 29 -- Received: by 10.42.141.136 with SMTP id o8mr2517668icu.512.1309454807321; 4, 29 -- Thu, 30 Jun 2011 10:26:47 -0700 (PDT) 4, 29 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.32.225]) 4, 29 -- by mx.google.com with ESMTPS id j7sm2371978icq.2.2011.06.30.10.26.45 4, 29 -- (version=TLSv1/SSLv3 cipher=OTHER); 4, 29 -- Thu, 30 Jun 2011 10:26:46 -0700 (PDT) 4, 29 -- Subject: stability of polystyrene 4, 29 -- From: Roseann Csencsits {rcsencsits-at-lbl.gov} 4, 29 -- Content-Type: text/plain; charset=us-ascii 4, 29 -- Message-Id: {2388CBC0-1E98-4982-836A-2DE0BEF1E865-at-lbl.gov} 4, 29 -- Date: Thu, 30 Jun 2011 10:26:41 -0700 4, 29 -- To: Microscopy-at-microscopy.com 4, 29 -- Mime-Version: 1.0 (Apple Message framework v1084) 4, 29 -- X-Mailer: Apple Mail (2.1084) 4, 29 -- Content-Transfer-Encoding: 8bit 4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p5UHQmuM006918 ==============================End of - Headers==============================
For the ones looking for a resource scheduler... Cirklo is proud to announce the release of Agendo v1.5.
Agendo is an open source (EUPL) resource scheduler developed by Cirklo at the Gulbenkian Institute of Science. This software was thought and partially developed by core facility managers with accumulated years of experience in research. This software is running in our facility for almost 2 years and we are continuously developing it so that other facilities can use it as well.
You can download it from http://github.com/Cirklo/CirkloPackage
just follow these instructions https://github.com/Cirklo/CirkloPackage/blob/master/INSTALL.txt
Contact us if you need help during the installation.
You can visit our demo site at http://www.cirklo.org/agendo_demo.php
USERNAME: admin PASSWORD: agendo
Go tohttp://www.cirklo.org/agendo_help.php to learn how to work with Agendo.
We can also set up and host a database for you in our server under a fee. Contactinfo-at-cirklo.org for more information.
Some features:
• Resource dependent configuration • Three different booking methods: pre-scheduling with administrator confirmation, pre-scheduling with user confirmation in situ and direct scheduling • Waiting lists with email or SMS warnings if the schedule is available • Personal calendar - each user is allowed to view past and future entries for all resources with permission to use • Warnings via email or SMS to the equipment admin if resource is scheduled but not used • Session comments directly sent through email to the equipment administrator • Color scheme that provides direct information about the equipment's usage • Easy invoicing reports • Static and dynamic reports (e.g. resource usage, unconfirmed entries, invoicing, most used resource, etc...) • Quick integration with Ekrano for live remote monitoring of equipment usage • Quick access to feature videos and help
More details inwww.cirklo.org
Best regards, João Lagarto
Equipment management Unit Instituto Gulbenkian de Ciência Rua da Quinta Grande, 6 2780-156 Oeiras Phone: + 351 214 464 607
==============================Original Headers============================== 18, 24 -- From jlagarto-at-cirklo.org Thu Jun 30 13:07:32 2011 18, 24 -- Received: from mx00.igc.gulbenkian.pt (mx00.igc.gulbenkian.pt [193.137.94.10]) 18, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p5UI7Wa2023833 18, 24 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jun 2011 13:07:32 -0500 18, 24 -- Received: from localhost (localhost [127.0.0.1]) 18, 24 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id 6D2082B60A4 18, 24 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jun 2011 19:07:31 +0100 (WEST) 18, 24 -- X-Virus-Scanned: Debian amavisd-new at igc.gulbenkian.pt 18, 24 -- Received: from mx00.igc.gulbenkian.pt ([127.0.0.1]) 18, 24 -- by localhost (igc.gulbenkian.pt [127.0.0.1]) (amavisd-new, port 10024) 18, 24 -- with LMTP id KCc08pFps3At for {microscopy-at-microscopy.com} ; 18, 24 -- Thu, 30 Jun 2011 19:07:13 +0100 (WEST) 18, 24 -- Received: from [172.22.31.231] (fw1dmz.igc.gulbenkian.pt [192.168.50.3]) 18, 24 -- by mx00.igc.gulbenkian.pt (Postfix) with ESMTP id AABDB2B608F 18, 24 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jun 2011 19:07:13 +0100 (WEST) 18, 24 -- Message-ID: {4E0CBB56.8040705-at-cirklo.org} 18, 24 -- Date: Thu, 30 Jun 2011 19:07:18 +0100 18, 24 -- From: =?windows-1252?Q?Jo=E3o_Lagarto?= {jlagarto-at-cirklo.org} 18, 24 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; pt-PT; rv:1.9.2.18) Gecko/20110616 Lightning/1.0b2 Thunderbird/3.1.11 18, 24 -- MIME-Version: 1.0 18, 24 -- To: microscopy-at-microscopy.com 18, 24 -- Subject: Agendo resource scheduler 18, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 18, 24 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both smythen-at-musc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: smythen-at-musc.edu Name: Nancy Smythe
Organization: Medical University of SC
Title-Subject: [Filtered] Perl's Fe stain with DAB
Message: I am looking for a protocol for enhancing Perl's staining with DAB. If anyone is currently doing this please contact me. It is to be on 5 micron paraffin sections.
oo Excerpt of used methods in that paper on Arabidopsis embryos (==} embedding in KULZER Technovit resin):
o Perls Stain and DAB/H2O2 Intensification The embryos were dissected from seeds previously imbibed in distilled water for 3 h, using a binocular magnifying lens. The isolated embryos were vacuum infiltrated with equal volumes of 4% (v/v) HCl and 4% (w/v) K-ferrocyanide (Perls stain solution) for 15 min and incubated for 30 min at room temperature (Stacey et al., 2008). The DAB intensification method is described in Meguro et al. (2007). After washing with distillated water, the embryos were incubated in a methanol solution containing 0.01 m NaN3 and 0.3% (v/v) H2O2 for 1 h, and then washed with 0.1 m phosphate buffer (pH 7.4). For the intensification reaction the embryos were incubated between 10 to 30 min in a 0.1 m phosphate buffer (pH 7.4) solution containing 0.025% (w/v) DAB (Sigma), 0.005% (v/v) H2O2, and 0.005% (w/v) CoCl2 (intensification solution). The reaction was stopped by rinsing with distilled water.The in vitro assay for FeII and FeIII was performed as follows: 1 mL of Perls stain solution was mixed with either 2 μL of FeSO4 or FeCl3 at 35 mm and, when necessary, 10 μL of a 57 mm ascorbic acid solution to reduce FeIII. The obtained solutions were dot blotted on a nitrocellulose membrane and further stained with DAB/H2O2 as described earlier for the staining of embryos. For Fe specificity assay of Perls/DAB stain (Fig. 1B, d) 1 mL of Perls stain solution was mixed with either 2 μL of FeSO4, FeCl3, CuSO4, MnCl2, or ZnCl2 at 35 mm, these solution were centrifuged, the supernantant discarded, and 1 mL of intensification solution was added and the tubes were vortexed. Finally, 2 μL were dot blotted on a nitrocellulose membrane.
o In Situ Perls/DAB/H2O2 Intensification The isolated embryos were vacuum infiltrated with fixation solution containing 2% (w/v) paraformaldehyde, 1% (v/v) glutaraldehyde, 1% (w/v) caffeine in 100 mm phosphate buffer (pH 7) for 30 min and incubated for 15 h in the same solution. The fixated embryos were washed with 0.1 m phosphate buffer (pH 7.4) three times, and dehydrated in successive baths of 50%, 70%, 90%, 95%, and 100% ethanol, butanol/ethanol 1:1 (v/v), and 100% butanol. Then, the embryos were embedded in the Technovit 7100 resin (Kulzer) according to the manufacturer's instructions and thin sections (3 μm) were made. The sections were deposited on glass slides that were incubated for 45 min in Perls stain solution. The intensification procedure was then applied as described above.Microscopic Analysis The embryos stained with Perls/DAB/H2O2 were dehydrated in successive baths of 50%, 70%, 90%, 95%, and 100% ethanol, butanol/ethanol 1:1 (v/v), and 100% butanol. Then, the embryos were embedded in the Technovit 7100 resin (Kulzer) according to the manufacturer's instructions and thin sections (7 μm) were made.
oo Lit. References in that paper concerning the methods used:
Meguro R, Asano Y, Odagiri S, Li C, Iwatsuki H, Shoumura K (2007) Nonheme-iron histochemistry for light and electron microscopy: a historical, theoretical and technical review. Arch Histol Cytol 70 1–19. [PubMed: http://www.ncbi.nlm.nih.gov/pubmed/17558140]
Stacey MG, Patel A, McClain WE, Mathieu M, Remley M, Rogers EE, Gassmann W, Blevins DG, Stacey G (2008) The Arabidopsis AtOPT3 protein functions in metal homeostasis and movement of iron to developing seeds. Plant Physiol 146 589–601. [PMC free article: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2245856/] [PubMed: http://www.ncbi.nlm.nih.gov/pubmed/18083798 ]
or even going back "to the roots": Histochemistry. 1980;66(3):239-44. ["Diaminobenzidine black" as a new histochemical demonstration of exogenous iron (author's transl)]. [Article in French] Nguyen-Legros J, Bizot J, Bolesse M, Pulicani JP. Abstract A new reaction for the histochemical demonstration of exogenous iron, used as a tracer for the study of connectivity in the central nervous system, is described. It consists of, first, the conversion of iron into Prussian blue, which acts secondarily as a catalyst for the oxidation of diaminobenzidine by hydrogen peroxide. The oxidized diaminobenzidine precipitates by polymerization and gives rise to a brown-colored insoluble reaction product. This reaction has a strikingly better sensitivity and contrast than the classical Perls's reaction. PMID:7399970[PubMed - indexed for MEDLINE==} http://www.ncbi.nlm.nih.gov/pubmed/7399970]
Best wishes, good luck and regards,
Wolfgang MUSS EM-Lab SALK-LKH & PMU Salzburg AUSTRIA
================================================================================================================ Von: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Gesendet: Freitag, 01. Juli 2011 03:17 An: Muß Wolfgang Betreff: [Microscopy] Perl's Fe stain with DAB
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
I am looking for a protocol for enhancing Perl's staining with DAB. If anyone is currently doing this please contact me. It is to be on 5 micron paraffin sections.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both zwang-at-genectr.hunter.cuny.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: zwang-at-genectr.hunter.cuny.edu Name: Zhong Wang
Organization: Hunter College
Title-Subject: [Filtered] Remote instrumentation
Message: My name is Zhong Wang working for the Bio-Imaging Facility at the Hunter College, CUNY. At present we are working on a remote instrumentation project for microscopic imaging. This project aims at manipulating microscopic systems from remote sites via the fast internet connection and supporting web conferencing software. By using proper remote connection protocols, microscopic imaging system can be shared remotely for research purposes. Now we are looking for partners to set up networks for tests and demos(we have confocal and spinning-disk system available for this purpose). The description of our facility can be found from the following link: http://biology.hunter.cuny.edu/index.php?option=com_content&view=article&id=44&Itemid=62 If you are interested, please send me an email including: Contact information Job Title
Your interest
Scientific Equipment that can be shared Internet Connection speed Does the remote end have any video conferencing codes? Thank you.
Zhong Wang Bio-imaging Facility Hunter College of CUNY
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alline.myers-at-nist.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] FIB/SEM/TEM Engineer Position
Message: FIB/SEM/TEM Engineer
NIST/Center for Nanoscale Science and Technology - Gaithersburg, MD 20899
Please send resumes to the address below: Vincent K. Luciani NanoFab Manager Center for Nanoscale Science and Technology National Institute of Standards and Technology 100 Bureau Drive, MS 6201 Gaithersburg, MD 20899-6200 USA
The Nanofabrication Facility (NanoFab) at the Center for Nanoscale Science and Technology (CNST) of the National Institute of Standards and Technology (NIST) seeks a highly motivated individual with significant experience in electron beam imaging and micro-/nano- fabrication technologies. The individual will have primary responsibility for the operation of multiple Focused Ion Beam (FIB) systems and a Field Emission Scanning Electron Microscope (FESEM), including process development, user training, operation and management of associated vendor service contracts and service activities. As part of a growing, state-of-the-art nanofabrication user facility, the successful candidate will possess a good ability to interact with and train users with skill levels varying from novice to expert. There will be extensive opportunities to expand experience into all facets of materials characterization and nanofabrication. Skills required: The individual should have significant practical experience in the FIB and FESEM operation, and a sound understanding of the associated analytic techniques such as EDS. Other desirable skills include: Experience specifically with FEI Helios FIBÂ’s, TEM sample preparation, TEM operation and Raith (SEM based) E-Beam lithography. The successful candidate will be proficient in MS Office applications and general computer use. Also important is a hands-on approach, an ability to work with a wide range of scientists and engineers, and an enthusiasm for working on a number of projects simultaneously. The ability to train and interact well with users is essential to supporting the user program. We will consider candidates at any appropriate level (pay band ZP I-IV, salary $21,592-$136,771). Candidates must have at least a bachelorÂ’s degree in science or engineering, or equivalent education combined with experience in FIB, SEM and TEM operation.
About the CNST NanoFab The NanoFab, part of NISTÂ’s Center for Nanoscale Science and Technology (http://cnst.nist.gov), is a 1,000 m2 Class 100 Cleanroom that contains new processing and analytical equipment and enables the fabrication of prototypical nanoscale test structures, measurement instruments, standard reference materials, electronic devices, magnetic devices, MEMS and bio-devices critical to NISTÂ’s nanotechnology programs. The NIST NanoFab is operated as a fee-based shared access user facility. This means that users from NIST and its partners are permitted to independently operate the equipment. NanoFab staff members also perform services for users.
About NIST: Located in Gaithersburg, MD, just outside Washington DC, the National Institute of Standards and Technology is a premier high technology government laboratory. An essential part of NIST's work is to anticipate the future. Fast-moving sectors such as nanotechnology, quantum information science, homeland security, information technology, and advanced manufacturing need sophisticated technical support systems to flourish and grow. NIST provides that support by continually improving the U.S. measurement system, developing new technologies, fostering standards, and providing both the business and technical evaluation tools needed to produce quality products and organizations. NIST is an unusual federal agency. Its mission is broad-to promote U.S. innovation and industrial competitiveness by advancing measurement science, standards, and technology in ways that enhance economic security and improve our quality of life. NIST recognizes two key assets: the diversity of the research programs within its multi-disciplinary structure and the diversity of the people it employs. Both provide strength and flexibility to meet the challenges of the future. It is this combination of talent, energy, commitment, and dedication that will keep the Agency on the frontiers of science. Many onsite services are available to our employees: free parking, child care, employee assistance, cafeterias, credit union, fitness center, and health services. Additionally, there are employee associations that sponsor various wellness, recreation, and social activities. For a summary of benefits available to NIST employees, go to http://www.nist.gov/hrmd/benefits/summarychart.htm.
The Department of Commerce is an Equal Opportunity Employer. US citizenship is required.
==============================Original Headers============================== 15, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 5 19:03:36 2011 15, 25 -- Received: from znl.com ([206.69.208.20]) 15, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6603amg024322 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jul 2011 19:03:36 -0500 15, 25 -- Received: from localhost (localhost [127.0.0.1]) 15, 25 -- by znl.com (Postfix) with ESMTP id 821AA3D4C6E 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jul 2011 19:03:36 -0500 (CDT) 15, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 15, 25 -- Received: from znl.com ([127.0.0.1]) 15, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 15, 25 -- with ESMTP id R4T7iRiyc5oz for {microscopy-at-microscopy.com} ; 15, 25 -- Tue, 5 Jul 2011 19:03:35 -0500 (CDT) 15, 25 -- Received: from NestorPwrBk.local (unknown [202.62.85.227]) 15, 25 -- by znl.com (Postfix) with ESMTPA id 94EF83D4C63 15, 25 -- for {microscopy-at-microscopy.com} ; Tue, 5 Jul 2011 19:03:34 -0500 (CDT) 15, 25 -- Message-ID: {4E13A654.2010206-at-microscopy.com} 15, 25 -- Date: Wed, 06 Jul 2011 05:33:32 +0530 15, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 15, 25 -- Reply-To: alline.myers-at-nist.gov 15, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 15, 25 -- MIME-Version: 1.0 15, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 25 -- Subject: viaWWW:FIB/SEM/TEM Engineer Position 15, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed 15, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From roy.goldup-at-tesco.net Wed Jul 6 18:49:48 2011 Return-Path: {roy.goldup-at-tesco.net} Received: from mtaout01-winn.ispmail.ntl.com (mtaout01-winn.ispmail.ntl.com [81.103.221.47]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p66NnWNK014355; Wed, 6 Jul 2011 18:49:33 -0500 Received: from aamtaout04-winn.ispmail.ntl.com ([81.103.221.35]) by mtaout01-winn.ispmail.ntl.com (InterMail vM.7.08.04.00 201-2186-134-20080326) with ESMTP id {20110706234932.QKZM10265.mtaout01-winn.ispmail.ntl.com-at-aamtaout04-winn.ispmail.ntl.com} ; Thu, 7 Jul 2011 00:49:32 +0100 Received: from web09-winn.ispmail.private.ntl.com ([81.103.221.25]) by aamtaout04-winn.ispmail.ntl.com (InterMail vG.3.00.04.00 201-2196-133-20080908) with ESMTP id {20110706234932.YTCQ25656.aamtaout04-winn.ispmail.ntl.com-at-web09-winn.ispmail.private.ntl.com} ; Thu, 7 Jul 2011 00:49:32 +0100 Message-ID: {20110707004932.C944G.569758.root-at-web09-winn.ispmail.private.ntl.com}
1) The Facilities Operation and Management Focus Interest Group (FOM FIG) hopes that everyone attending M&M 2011 will make plans to attend the Symposium/Roundtable that will be presented on Wednesday from 1:30 to 3:30 in Room 109 of the Nashville Convention Center. We think that the topic is of interest to many.
A14B Equipment Funding Opportunities & Strategies for Success Owen Mills and Christopher Gilpin With reduction in government funding it has become increasingly competitive to obtain grant funding from federal funding agencies. Agency budgets have been reduced and criteria for successful submissions evolve over time. This session will consist of a set of informal presentations, in an open forum format, by representatives from federal funding agencies Office of Naval Research, National Institutes of Health and the National Science Foundation. Each speaker will give an overview of their process for announcing funding opportunities together with a description the application and review process. Each presentation will specifically address programs and procedures that pertain to acquiring major equipment that will be relevant to society members. In addition, speakers will cover strategies to write a successful application that could improve funding prospects. The open format will allow for audience participation and the session is planned to be fluid and dynamic to enable individual components of the grant application process to be explored in more detail especially where common mistakes are often made. Invited Speakers: Debby Sherman, Purdue University Shahbazian Yassar Reza, Michigan Technological University Richard Edelmann, Miami University Isabel Cardenas-Navia, Office of Naval Research Craig Henderson, National Science Foundation Christina Liu, National Institutes of Health
2) Any day now our Facilities Operation and Management Focus Interest Group web page will be activated. We will give you the link and information very soon. [We hope!]
3) The FOM FIG welcomes new members and if you think you are a member and have not received a previous message concerning the symposium and the Annual Luncheon and Business Meeting of the Facilities Operation and Management Focus Interest Group (FOM FIG) your membership has expired. You can join by contacting the MSA business office to pay the $10.00 FIG fee. Please also contact Pat Connelly at {psconnelly-at-gmail.com} if you are joining now or have questions.
4) FOM FIG Members shall have their Annual Luncheon and Business Meeting this year during M&M 2011 on Tuesday August 9. The Lunch will be provided at no cost to our members. There is however a need for you to reply to Pat Connelly as soon as possible deadline is July 15th so do NOT hit ³Reply² but please email {psconnelly-at-gmail.com} for your Reservation must be made before then.
Owen Mills, Leader of the FOM FIG, will lead the meeting. During the Business Meeting we will be having Elections for a Leader-Elect to follow Tom Williams, soon to become the Leader. We are also in need of a Secretary. The current officers request that you nominate yourself to serve in either of these two capacities by contacting Pat Connelly. There is a lot of cooperation between past office holders and the newly elected and there is time to get familiar with the duties. You will not be thrown to the wolves so to speak.
The discussion topic to follow the business meeting is: Deferred Maintenance: Core Facilities and the implications of aging infrastructure in the era of shrinking budgets -what are the short-term and long-term problems for Labs -innovative solutions and "band-aid" fixes
See you in Nashville!
Owen P. Mills, FOM FIG Leader Pat Connelly, FOM FIG Secretary {psconnelly-at-gmail.com}
==============================Original Headers============================== 9, 27 -- From connellyps-at-nhlbi.nih.gov Wed Jul 6 19:46:23 2011 9, 27 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p670kNNa014874 9, 27 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jul 2011 19:46:23 -0500 9, 27 -- X-IronPortListener: Outbound_SMTP 9, 27 -- X-IronPort-Anti-Spam-Filtered: true 9, 27 -- X-IronPort-Anti-Spam-Result: AuIGAKr9FE6cKEcW/2dsb2JhbABTnDKLWXAHrE+EE48ii22GNwSJAYlAhReENocM 9, 27 -- Received: from unknown (HELO NIHHT03.nih.gov) ([156.40.71.22]) 9, 27 -- by nihxway4out.hub.nih.gov with ESMTP; 06 Jul 2011 20:46:22 -0400 9, 27 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT03.nih.gov 9, 27 -- ([156.40.71.22]) with mapi; Wed, 6 Jul 2011 20:46:22 -0400 9, 27 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 9, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 27 -- Date: Wed, 6 Jul 2011 20:46:22 -0400 9, 27 -- Subject: Facilities Operation and Management Focus Interest Group News 9, 27 -- Thread-Topic: Facilities Operation and Management Focus Interest Group News 9, 27 -- Thread-Index: Acw8P0qnZm/dnhVrSpGLIYCJNts3nA== 9, 27 -- Message-ID: {CA3A7A1E.858D%connellyps-at-nhlbi.nih.gov} 9, 27 -- Accept-Language: en-US 9, 27 -- Content-Language: en 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- acceptlanguage: en-US 9, 27 -- Content-Type: text/plain; charset="iso-8859-1" 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p670kNNa014874 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Edward.Duke-at-sdsmt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Edward.Duke-at-sdsmt.edu Name: Ed Duke
Organization: South Dakota School of Mines and Technology
Title-Subject: [Filtered] JEOL 840A SEM surplus
Message: JEOL 840 SEM with Oxford ISIS EDS and heating/cooling stage is available through online auction site PublicSurplus.com
Do you know any way to concentrate the bacteria to about 1 ul for observation on a slide-well?
I have a 1 ml aqueous solution containing about 10 bacteria cells. So that I can observe the bacteria cells, I am centrifuging the 1 ml, removing the supernatant and spotting 10 ul to a 0.6 mm diameter slide-well before staining with DAPI.
Unfortunately the 10 ul volume and well area is too large at 60X objective to quickly find the stained bacteria. It would be much better I centrifuge the volume still smaller to 1 ul, but this is difficult to spot without losing some of the bacteria. I have tried filtering, but the filter area is still too large for rapid detection.
Regards,
Tom
-- Tom Lawson PhD Student tomxlawson-at-gmail.com Macquarie University NSW, 2109 Australia
==============================Original Headers============================== 7, 24 -- From tomxlawson-at-gmail.com Thu Jul 7 02:34:19 2011 7, 24 -- Received: from mail-gw0-f41.google.com (mail-gw0-f41.google.com [74.125.83.41]) 7, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p677YJcx021806 7, 24 -- for {Microscopy-at-Microscopy.Com} ; Thu, 7 Jul 2011 02:34:19 -0500 7, 24 -- Received: by gwaa12 with SMTP id a12so335946gwa.0 7, 24 -- for {Microscopy-at-Microscopy.Com} ; Thu, 07 Jul 2011 00:34:18 -0700 (PDT) 7, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 24 -- d=gmail.com; s=gamma; 7, 24 -- h=mime-version:date:message-id:subject:from:to:content-type; 7, 24 -- bh=q/3VXCHaURo8Etw3pIFWLCHDQHHP/E5MeuEiPt7ddNI=; 7, 24 -- b=oYc4GRtix9CR4BfVElHRAGiDVn1IPXW66+eWTd5GftIlsLTFCFKBuBBV3gKwlGFVRV 7, 24 -- tMFWpHFhFQaqEmEsF/Meic+yseDbeNdLHd6w9ootsHjCMn/XF0l+RuMLhHQvluhOkyfx 7, 24 -- pPfr1vzUCWMt4gEfVkORVYjWqNqElR//xEm6w= 7, 24 -- MIME-Version: 1.0 7, 24 -- Received: by 10.236.125.161 with SMTP id z21mr568743yhh.124.1310024058783; 7, 24 -- Thu, 07 Jul 2011 00:34:18 -0700 (PDT) 7, 24 -- Received: by 10.147.36.27 with HTTP; Thu, 7 Jul 2011 00:34:18 -0700 (PDT) 7, 24 -- Date: Thu, 7 Jul 2011 17:34:18 +1000 7, 24 -- Message-ID: {CA+UEVcvVPGmbhskzdsqWBBun823-Ac29dv0eUdhQVZ=OpKxwMA-at-mail.gmail.com} 7, 24 -- Subject: Any way to concentrate the bacteria to about 1 ul for observation on 7, 24 -- a slide-well? 7, 24 -- From: Tom Lawson {tomxlawson-at-gmail.com} 7, 24 -- To: Microscopy-at-Microscopy.Com 7, 24 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
If you are planning to attend the forthcoming M&M 2011 meeting in Nashville, and have an interest in High Pressure Freezing, please consider attending the following user group meeting:
High Pressure Freezer User Group Meeting
Tuesday Aug 9th, 2011 6:00pm until around 8:00pm Gospel Room, Renaissance Nashville Hotel 611 Commerce Street Nashville (adjacent to the Convention Center)
Meeting Philosophy: An informal, relaxed discussion group sharing experiences with high pressure freezing of biological materials. Although this meeting is primarily focused on experiences with the HPM 010 High Pressure Freezing Machine, it is open to anyone with an interest in cryoimmobilization by high pressure freezing or with experiences to share using other freezing devices and associated cryo techniques such as freeze substitution.
Meeting Co-Chairs: Joe Austin, PhD Director-Advanced Electron Microscopy Facility Biological Science Division University of Chicago
Frank Macaluso Director - Analytical Imaging Facility Albert Einstein College of Medicine Bronx, NY
Meeting Agenda:
6:00pm Welcome & Introduction
6:15pm - 6:45pm "How I learned to Stop Worrying and Trust my TEM Images" - a brief introduction to high pressure freezing and freeze substitution Joe Austin, PhD
6:45pm - 7:15pm " Neutral lipids: a moving target for preservation in freeze substitution of plant cells" R. Howard Berg, PhD
Director-Integrated Microscopy Facility Danforth Plant Science Center St. Louis
7:15pm - 8:00pm (open): Open discussion
Complimentary soft drinks, light refreshments, coffee and tea will be served plus a cash bar will be open during the meeting serving wine, beer and cocktails.
Please drop me an email if you would like to reserve a place.
See you in Nashville.
Dave Roberts Director-RMC Products Boeckeler Instruments Inc 4650 S. Butterfield Drive Tucson, Arizona 85714 Tel: 520-745-0001 Fax: 520-745-0004 www.rmcproducts.com dave-at-boeckeler.com
==============================Original Headers============================== 17, 15 -- From dave-at-boeckeler.com Fri Jul 8 17:54:59 2011 17, 15 -- Received: from spamstopper.cnsusa.com ([209.104.7.157]) 17, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p68MsvNN010437 17, 15 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jul 2011 17:54:58 -0500 17, 15 -- Received: from [10.0.1.241] [207.224.156.250] by spamstopper.cnsusa.com with ESMTP 17, 15 -- (SMTPD-10.02) id AAAC05B8; Fri, 08 Jul 2011 15:54:36 -0700 17, 15 -- Message-ID: {4E178AA3.5070109-at-boeckeler.com} 17, 15 -- Date: Fri, 08 Jul 2011 15:54:27 -0700 17, 15 -- From: Dave Roberts {dave-at-boeckeler.com} 17, 15 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.9) Gecko/20100825 Thunderbird/3.1.3 17, 15 -- MIME-Version: 1.0 17, 15 -- To: microscopy-at-microscopy.com 17, 15 -- Subject: High Pressure Freezer User Group Meeting 17, 15 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 15 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yamawaki-at-stanford.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I still live in the dark ages of printing micrographs. My Durst Varicontrol is no longer varicontroling. I know it is not the bulb because I have tried two different new bulbs. If you have a Durst Varicontrol available please contact me. The enlarger is Durst Femokit.
Thank you.
Ruth Yamawaki Stanford University Department of Comparative Medicine Palo Alto, Ca 650 723-3457
Dear Ruth, if you are still doing b+w prints and need to repair stuff, why not add little comfort and changing to a better controller and get results faster, easier and better looking? I am using the splitgrade-system. It fits on a lot of colour or multigrade enlargers but works best when the enlarger head is re-worked containing motorized dichroitic filters (you can bring in the filters manually also...). See more at http://www.heilandelectronic.de/html/english/products/splitgrade_main.htm
Just a satisfied customer.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 09.07.11 05:41, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both yamawaki-at-stanford.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: yamawaki-at-stanford.edu Name: Ruth Yamawaki } } Organization: Stanford University } } Title-Subject: [Filtered] Durst varicontroller wanted } } Message: I still live in the dark ages of printing micrographs. My } Durst Varicontrol is no longer varicontroling. I know it is not the bulb } because I have tried two different new bulbs. If you have a Durst } Varicontrol available please contact me. The enlarger is Durst Femokit. } } } Thank you. } } Ruth Yamawaki } Stanford University } Department of Comparative Medicine } Palo Alto, Ca } 650 723-3457 } } Login Host: 171.65.84.72 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Jul 8 22:37:44 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p693bi4F029222 } 11, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jul 2011 22:37:44 -0500 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id 6F5493D741F } 11, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jul 2011 22:37:44 -0500 (CDT) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 25 -- with ESMTP id m6AUoiASM0YF for {microscopy-at-microscopy.com} ; } 11, 25 -- Fri, 8 Jul 2011 22:37:41 -0500 (CDT) } 11, 25 -- Received: from NestorPwrBk.local (unknown [202.62.85.227]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id 8F8AB3D7414 } 11, 25 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jul 2011 22:37:40 -0500 (CDT) } 11, 25 -- Message-ID: {4E17CD01.1000109-at-microscopy.com} } 11, 25 -- Date: Sat, 09 Jul 2011 09:07:37 +0530 } 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 11, 25 -- Reply-To: yamawaki-at-stanford.edu } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: viaWWW:Durst varicontroller } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 22 -- From stefan.diller-at-t-online.de Sat Jul 9 01:32:08 2011 7, 22 -- Received: from mailout03.t-online.de (mailout03.t-online.de [194.25.134.81]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p696W8v2015006 7, 22 -- for {microscopy-at-microscopy.com} ; Sat, 9 Jul 2011 01:32:08 -0500 7, 22 -- Received: from fwd07.aul.t-online.de (fwd07.aul.t-online.de ) 7, 22 -- by mailout03.t-online.de with smtp 7, 22 -- id 1QfR5D-0005JA-DN; Sat, 09 Jul 2011 08:32:07 +0200 7, 22 -- Received: from mac-pro.speedport.ip (SrJtXEZcohHRvwqaWipMHjnCNxH8npWTbnt1ckYkF-9XgvJlJOEC+-cokqZvnlbguN-at-[93.222.107.184]) by fwd07.t-online.de 7, 22 -- with esmtp id 1QfR59-0M1pvE0; Sat, 9 Jul 2011 08:32:03 +0200 7, 22 -- Message-ID: {4E17F5E3.5080006-at-t-online.de} 7, 22 -- Date: Sat, 09 Jul 2011 08:32:03 +0200 7, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 22 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; de; rv:1.9.2.18) Gecko/20110616 Thunderbird/3.1.11 7, 22 -- MIME-Version: 1.0 7, 22 -- To: microscopy-at-microscopy.com 7, 22 -- Subject: Re: [Microscopy] viaWWW:Durst varicontroller 7, 22 -- References: {201107090341.p693faYb002900-at-ns.microscopy.com} 7, 22 -- In-Reply-To: {201107090341.p693faYb002900-at-ns.microscopy.com} 7, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 22 -- Content-Transfer-Encoding: 7bit 7, 22 -- X-ID: SrJtXEZcohHRvwqaWipMHjnCNxH8npWTbnt1ckYkF-9XgvJlJOEC+-cokqZvnlbguN 7, 22 -- X-TOI-MSGID: 566de315-cfcf-4ef1-ac7a-7b9ce4c236f8 ==============================End of - Headers==============================
I've just been reading about an experiment exploring the use of a 229 Th (thorium) *nuclear* transition at 7.6 eV that might form the basis of a precise clock (NewScientist website).
However, what intrigued me was this: has this transition ever been seen in the electron microscope (band-gap region)? If so, would it work well as the basis of crystal-field splitting measurements, and would its excitation depend sensitively on the excitation of the 1s Bloch states?
Best, Jon
==============================Original Headers============================== 5, 25 -- From jsb43-at-hermes.cam.ac.uk Mon Jul 11 07:51:11 2011 5, 25 -- Received: from ppsw-51.csi.cam.ac.uk (ppsw-51.csi.cam.ac.uk [131.111.8.151]) 5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6BCpBK7002168 5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 07:51:11 -0500 5, 25 -- X-Cam-AntiVirus: no malware found 5, 25 -- X-Cam-SpamDetails: not scanned 5, 25 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 5, 25 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:51643) 5, 25 -- by ppsw-51.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.158]:25) 5, 25 -- with esmtpa (EXTERNAL:jsb43) id 1QgFx8-0005Nw-Wu (Exim 4.72) for Microscopy-at-microscopy.com 5, 25 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Mon, 11 Jul 2011 13:51:10 +0100 5, 25 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk) 5, 25 -- with local (PRAYER:jsb43) id 1QgFx8-00030A-68 (Exim 4.67) for Microscopy-at-microscopy.com 5, 25 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Mon, 11 Jul 2011 13:51:10 +0100 5, 25 -- Received: from [128.232.229.150] by webmail.hermes.cam.ac.uk 5, 25 -- with HTTP (Prayer-1.3.4); 11 Jul 2011 13:51:10 +0100 5, 25 -- Date: 11 Jul 2011 13:51:10 +0100 5, 25 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 5, 25 -- To: MSA Listserver {Microscopy-at-microscopy.com} 5, 25 -- Subject: 229 Th transition in EELS? 5, 25 -- Message-ID: {Prayer.1.3.4.1107111351100.1173-at-hermes-2.csi.cam.ac.uk} 5, 25 -- X-Mailer: Prayer v1.3.4 5, 25 -- Mime-Version: 1.0 5, 25 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 5, 25 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
Did anyone take a trouble of putting together some kind of tutorial, or any other form of programing aid, or a cheat-sheet for FEI AutoScript language? I am looking into the project which will require automation, and could use some help in the beginning.
We have the "AutoScript Technical Note" which came with the tool, which is helpful, but that is rather a reference then the manual or a tutorial...
Thanks everyone beforehand, -- Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
==============================Original Headers============================== 4, 27 -- From vray-at-partbeamsystech.com Mon Jul 11 12:16:07 2011 4, 27 -- Received: from nm19.bullet.mail.sp2.yahoo.com (nm19.bullet.mail.sp2.yahoo.com [98.139.91.89]) 4, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p6BHG5DN024735 4, 27 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 12:16:06 -0500 4, 27 -- Received: from [98.139.91.61] by nm19.bullet.mail.sp2.yahoo.com with NNFMP; 11 Jul 2011 17:16:05 -0000 4, 27 -- Received: from [98.139.91.60] by tm1.bullet.mail.sp2.yahoo.com with NNFMP; 11 Jul 2011 17:16:05 -0000 4, 27 -- Received: from [127.0.0.1] by omp1060.mail.sp2.yahoo.com with NNFMP; 11 Jul 2011 17:16:05 -0000 4, 27 -- X-Yahoo-Newman-Id: 43590.91144.bm-at-omp1060.mail.sp2.yahoo.com 4, 27 -- Received: (qmail 26978 invoked from network); 11 Jul 2011 17:16:04 -0000 4, 27 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1310404564; bh=1z/GeswJoCbop3JIqtk20v/+3Z36EI58NrgPZJlGA8I=; h=Received:X-Yahoo-SMTP:X-YMail-OSG:X-Yahoo-Newman-Property:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:Content-Type:Content-Transfer-Encoding; b=6TmfPAPuynQWUpOpw4dJk5vrPcd94/OuifG1/9m9O0EJ9LLf0sMxz15lCLbHm5DUsgzGtMmRB6ntRAhuW5uWZUWA1CAtL9mrEyxMEV7y5ahDY9IlGS65Q+7HY3uBbdgPSA01YeHbAM8fKVFjgTTi43CP0nG77cGyy51j8Qn7bQI= 4, 27 -- Received: from [192.168.2.2] (vray-at-75.68.231.25 with plain) 4, 27 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 11 Jul 2011 10:16:03 -0700 PDT 4, 27 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 27 -- X-YMail-OSG: a6CdGrwVM1lfowtSmo88dpCdAWaz0X6Or1AlnrHVmfBA1vB 4, 27 -- 312E- 4, 27 -- X-Yahoo-Newman-Property: ymail-3 4, 27 -- Message-ID: {4E1B2FD4.6080604-at-partbeamsystech.com} 4, 27 -- Date: Mon, 11 Jul 2011 13:16:04 -0400 4, 27 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 27 -- Reply-To: vray-at-partbeamsystech.com 4, 27 -- Organization: PBS&T 4, 27 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Lightning/1.0b2 Thunderbird/3.1.11 4, 27 -- MIME-Version: 1.0 4, 27 -- To: microscopy-at-microscopy.com 4, 27 -- Subject: Tutorial or "How To" guide for FEI "Auto Script" languate 4, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I have 2 MT-2s that need a home. I'm not sure if they still work but if you want them and are willing to pick them up or pay for shipping you can have them.
First come, first served. These are "As Is".
Contact me if you are interested.
Paula :-)
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 7, 26 -- From patpxs-at-gmail.com Mon Jul 11 13:04:13 2011 7, 26 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6BI4D2u009201 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 13:04:13 -0500 7, 26 -- Received: by iwn8 with SMTP id 8so4534824iwn.0 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 11:04:12 -0700 (PDT) 7, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 26 -- d=gmail.com; s=gamma; 7, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 7, 26 -- :content-transfer-encoding; 7, 26 -- bh=/4mFj4vhhvbsMb9Ux/ox4iTYgovi6h20V/XMx50Ml8Y=; 7, 26 -- b=t2S64N2DwNWX1oKyNPe/zNuNoQr25YVy2LcMrUSCWSgRJnh9o17SSfqLnKSsYfoG+P 7, 26 -- jpyg+y1LO7n3HC+uG0bhiyvr5GgFIry0YApPwLaLBlCDukWvUfIvm/fgepaA3F6EudGX 7, 26 -- ezhFxBOpG3qq/XNuUqz20GnnpeKk61pqRL5s0= 7, 26 -- MIME-Version: 1.0 7, 26 -- Received: by 10.43.53.9 with SMTP id vo9mr3178684icb.270.1310407452696; Mon, 7, 26 -- 11 Jul 2011 11:04:12 -0700 (PDT) 7, 26 -- Received: by 10.42.229.10 with HTTP; Mon, 11 Jul 2011 11:04:12 -0700 (PDT) 7, 26 -- Date: Mon, 11 Jul 2011 14:04:12 -0400 7, 26 -- Message-ID: {CAPSjddbwE3Xm_7mEtfxqBoTt2NQW6R5O8_++Y54xk4=gSC-hiA-at-mail.gmail.com} 7, 26 -- Subject: MT-2 Free To Good Home 7, 26 -- From: Paula Sicurello {patpxs-at-gmail.com} 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- Content-Type: text/plain; charset=ISO-8859-1 7, 26 -- Content-Transfer-Encoding: 8bit 7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6BI4D2u009201 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both benjamin.brezler-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: benjamin.brezler-at-gmail.com Name: Benjamin Brezler
Organization: San Francisco State University
Title-Subject: [Filtered] Bacterial Specimen Dehydration Help for SEM
Message: I am having some difficulty with dehydration of my specimens, and help I could get would be much appreciated.
I am working with a Gram-negative, motile, alpha-proteobacteria. My general protocol is as follows; 1) Fix for 15min. at room temp. with 2.5% glutaraldehyde in a 0.1M Sodium Cacodylate solution (pH~7). 2) Dehydrate with a ethanol series--30%, 50%, 70%, 100% (X3), then critical point dry.
Unfortunately, my bacteria are coming out wrinkled, shriveled, and just generally improperly dehydrated.
I have tried several permutations of this protocol including fixing at in the fridge for an hour, post-fixing with 1% Osmium Tetroxide for 30min. before the dehydration process, but I haven't found anything that has really helped.
Does anyone have advice/experience with this sort of preparation?
When I have problems, I buckle down and do what Dennis Kunkel does (Google his stuff to see beautiful microbes):
Your fix is OK, wash well, postfix 1% OsO4, wash with cacodylate again, then (I think this is the trick) dehydrate in ethanol, 10%, 20%, 30%, 50%, 70%, 85%, 95%, two times each dilution; first time for 5 in, second time for 15 min. Don't rush this part.
When in 70% ethanol transfer to filter holder (or smooth lens tissue origami packets).
Dehydrate in 100% ethanol 3 x 10 min, then critical point dry
Good luck! Aloha, Tina } } Message: I am having some difficulty with dehydration of my specimens, } and help I could get would be much appreciated. } } I am working with a Gram-negative, motile, alpha-proteobacteria. My } general protocol is as follows; } 1) Fix for 15min. at room temp. with 2.5% glutaraldehyde in a 0.1M } Sodium Cacodylate solution (pH~7). } 2) Dehydrate with a ethanol series--30%, 50%, 70%, 100% (X3), then } critical point dry. } } Unfortunately, my bacteria are coming out wrinkled, shriveled, and just } generally improperly dehydrated. } } I have tried several permutations of this protocol including fixing at } in the fridge for an hour, post-fixing with 1% Osmium Tetroxide for } 30min. before the dehydration process, but I haven't found anything that } has really helped. } } Does anyone have advice/experience with this sort of preparation? } } } Thanks, } Benjamin } } Login Host: 98.234.237.9 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 14, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 11 21:29:34 2011 } 14, 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6C2TYmt031604 } 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 21:29:34 -0500 } 14, 25 -- Received: from localhost (localhost [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id 942B63D98F2 } 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 21:29:34 -0500 (CDT) } 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 14, 25 -- with ESMTP id bWmVb6nCI32l for {microscopy-at-microscopy.com} ; } 14, 25 -- Mon, 11 Jul 2011 21:29:34 -0500 (CDT) } 14, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 14, 25 -- by znl.com (Postfix) with ESMTPA id 03C723D98E7 } 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 21:29:34 -0500 (CDT) } 14, 25 -- Message-ID: {4E1BB18D.8050501-at-microscopy.com} } 14, 25 -- Date: Mon, 11 Jul 2011 21:29:33 -0500 } 14, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 14, 25 -- Reply-To: benjamin.brezler-at-gmail.com } 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 14, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 25 -- Subject: viaWWW:Bacterial Specimen Dehydration Help for SEM } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 23 -- From tina-at-pbrc.hawaii.edu Mon Jul 11 22:03:33 2011 7, 23 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6C33WBP015734 7, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 22:03:32 -0500 7, 23 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 23 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p6C33O8d018580 7, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 23 -- Mon, 11 Jul 2011 17:03:24 -1000 (HST) 7, 23 -- Received: from localhost (tina-at-localhost) 7, 23 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p6C33N6H018576; 7, 23 -- Mon, 11 Jul 2011 17:03:24 -1000 (HST) 7, 23 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 7, 23 -- Date: Mon, 11 Jul 2011 17:03:23 -1000 (HST) 7, 23 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 23 -- X-X-Sender: tina-at-b1000 7, 23 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} , 7, 23 -- benjamin.brezler-at-gmail.com 7, 23 -- Subject: Re: [Microscopy] viaWWW:Bacterial Specimen Dehydration Help for SEM 7, 23 -- In-Reply-To: {201107120230.p6C2UkXN000393-at-ns.microscopy.com} 7, 23 -- Message-ID: {Pine.GSO.4.64.1107111657390.13650-at-b1000} 7, 23 -- References: {201107120230.p6C2UkXN000393-at-ns.microscopy.com} 7, 23 -- MIME-Version: 1.0 7, 23 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Your fix seems short to me - I generally use 30-60 minutes in 1% to 1.25% glut in buffer. The dehydration definitely needs expanding - especially the lack of 90% and 95% EtOH. But. Mostly, what do you mean by "critical point dry"? That is, what is your exact procedure? You have to think of the CPD in the same way as you do the dehydration. The EtOH must be replaced within the cells with liquid CO2, which requires flushing and soaking cycles, analogous to the dehydration steps used to substitute H2O with EtOH. I generally use 5, 5 minute soaks when doing bacteria on membranes. If I'm doing cells on agar, I treat them as tissue - the agar has to be dried properly to maintain the relationships between the cells (they're a community, not just a bunch of bugs). The time and number of steps varies with the cells, the community (how many different types, what kind of biofilm, etc.), and is pretty much empirical. Have you tried drying from HMDS (hexamethyldisilizane)? That can work very nicely. And some bacteria will air dry from 100% EtOH or acetone just fine.
Phil
} Email: benjamin.brezler-at-gmail.com Name: Benjamin Brezler } } Organization: San Francisco State University } } Title-Subject: [Filtered] Bacterial Specimen Dehydration Help for SEM } } Message: I am having some difficulty with dehydration of my specimens, } and help I could get would be much appreciated. } } I am working with a Gram-negative, motile, alpha-proteobacteria. My } general protocol is as follows; } 1) Fix for 15min. at room temp. with 2.5% glutaraldehyde in a 0.1M } Sodium Cacodylate solution (pH~7). } 2) Dehydrate with a ethanol series--30%, 50%, 70%, 100% (X3), then } critical point dry. } } Unfortunately, my bacteria are coming out wrinkled, shriveled, and just } generally improperly dehydrated. } } I have tried several permutations of this protocol including fixing at } in the fridge for an hour, post-fixing with 1% Osmium Tetroxide for } 30min. before the dehydration process, but I haven't found anything that } has really helped. } } Does anyone have advice/experience with this sort of preparation? } } } Thanks, } Benjamin
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 28 -- From oshel1pe-at-cmich.edu Tue Jul 12 07:15:37 2011 5, 28 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6CCFbWS012362 5, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 07:15:37 -0500 5, 28 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 5, 28 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p6CBrqPj011251; 5, 28 -- Tue, 12 Jul 2011 07:53:52 -0400 5, 28 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 28 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.270.1; Tue, 12 Jul 5, 28 -- 2011 08:15:35 -0400 5, 28 -- MIME-Version: 1.0 5, 28 -- Message-ID: {a06240801ca41e96863db-at-[141.209.160.249]} 5, 28 -- In-Reply-To: {201107120233.p6C2XGmX004327-at-ns.microscopy.com} 5, 28 -- References: {201107120233.p6C2XGmX004327-at-ns.microscopy.com} 5, 28 -- Date: Tue, 12 Jul 2011 08:15:32 -0400 5, 28 -- To: {Microscopy-at-microscopy.com} 5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 28 -- Subject: Re: [Microscopy] viaWWW:Bacterial Specimen Dehydration Help for 5, 28 -- SEM 5, 28 -- CC: {benjamin.brezler-at-gmail.com} 5, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 28 -- X-Originating-IP: [141.209.160.249] 5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 28 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 5, 28 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 28 -- X-CanItPRO-Stream: default 5, 28 -- X-Canit-Stats-ID: 03F6XRQCs - eea98c9495a1 - 20110712 5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
We have a Cambridge Stereoscan 100 SEM (~1982) which was dismantled a few years ago, the "hardware" part dealing now as model of how a SEM is built, in the entry hall of our EM facility.
We have still the electronics laying in the way, which must be discard soon and is so available if someone is interested. It's complete exept the turbo pump controler.
Please contacte me, or caroline.habold-at-iphc.cnrs.fr, but please before july 22. I'll be away after that. It's located in Strasbourg, France, and of coarse is are free of charge expet shipping and packing at your charge. It will be discard beginning september.
Best regards
Jacques
-- Veuillez prendre note de la nouvelle adresse mail.
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
We are looking for a BSE detector for a JEOL840 A (or any backscatter detector that might work). One of our students raised a sample just a little too high! If anyone has one sitting around gathering dust they would be willing to part with we would be forever grateful.
TIA
Matt Duley
Matthew L. Duley
Microscopy Specialist Center for Advanced Microscopy & Imaging 9 Upham Hall Miami University Oxford, Ohio 45056 513.529.4164 FAX 513.529.4243 Duleyml-at-muohio.edu
==============================Original Headers============================== 9, 18 -- From duleyml-at-muohio.edu Tue Jul 12 10:46:13 2011 9, 18 -- Received: from mualmarp02.mcs.muohio.edu (mualmarp02.mcs.muohio.edu [134.53.6.74]) 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6CFkC7u015868 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 10:46:12 -0500 9, 18 -- Received: from mualmafp02.mcs.muohio.edu (mualmafp02.mcs.muohio.edu [134.53.6.6]) 9, 18 -- by mualmarp02.mcs.muohio.edu (8.13.8/8.13.8) with ESMTP id p6CFkCbk015446 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 11:46:12 -0400 9, 18 -- Received: from EMF07.muohio.edu ([10.34.160.229]) 9, 18 -- by mualmafp02.mcs.muohio.edu (8.13.8/8.13.8) with ESMTP id p6CFkCPP012316 9, 18 -- for {microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 11:46:12 -0400 9, 18 -- Message-Id: {7.0.1.0.2.20110712114458.028830b8-at-muohio.edu} 9, 18 -- X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 9, 18 -- Date: Tue, 12 Jul 2011 11:46:12 -0400 9, 18 -- To: microscopy-at-microscopy.com 9, 18 -- From: Matthew Duley {duleyml-at-muohio.edu} 9, 18 -- Subject: need a BSE detector for JEOL 840 9, 18 -- Mime-Version: 1.0 9, 18 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
I do an extended fixation in glut (1 day) and Os (1-2 days), followed by dehydration in EtOH, like Tina describes.
Then, a long exchange of CO2 in the CPD device, like Phil mentions.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901
==============================Original Headers============================== 5, 18 -- From bozzola-at-siu.edu Tue Jul 12 11:10:13 2011 5, 18 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6CGACJM032340 5, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 11:10:12 -0500 5, 18 -- Received: by iyl8 with SMTP id 8so5630905iyl.0 5, 18 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 09:10:12 -0700 (PDT) 5, 18 -- MIME-Version: 1.0 5, 18 -- Received: by 10.42.150.71 with SMTP id z7mr36345icv.474.1310487012011; Tue, 12 5, 18 -- Jul 2011 09:10:12 -0700 (PDT) 5, 18 -- Received: by 10.43.50.138 with HTTP; Tue, 12 Jul 2011 09:10:12 -0700 (PDT) 5, 18 -- Date: Tue, 12 Jul 2011 11:10:12 -0500 5, 18 -- Message-ID: {CAKeCJ9xZo8gxAXHP+67ffYe6zEyHJ90tmDYmxyMa8jXDBoabkA-at-mail.gmail.com} 5, 18 -- Subject: Re: Bacterial Specimen Dehydration Help for 5, 18 -- From: John Bozzola {bozzola-at-siu.edu} 5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 18 -- Content-Type: text/plain; charset=UTF-8 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6CGACJM032340 ==============================End of - Headers==============================
Ben, Joining in the chorus of excellent responses you've gotten so far, here are a couple of thoughts:
} 1) Fix for 15min. at room temp. with 2.5% glutaraldehyde in a 0.1M } Sodium Cacodylate solution (pH~7).
In my hands, short ( {4 hrs -at- RT) fixations of bacteria can cause later shrinkage problems. Admittedly I usually work with Gram-positives, but even Gram-negative bugs often need at least an overnight fix. If you're trying to maintain any extracellular matrix, this problem becomes even more acute - 16-24 hrs at RT is where I usually start.
Though not usually an issue with single bacterial cells, the osmolality of your fix is pretty low, too.
} 2) Dehydrate with a ethanol series--30%, 50%, 70%, 100% (X3), then } critical point dry.
As other commentators have noted, this EtOH series is short and pretty abrupt: I've moved toward more steps rather than fewer (I typically do eight gradations - 25% -} 50% -} 70% -} 85% -} 95% x2 -} 100% x2). Even this may not be enough: I'm considering moving to 10 stages (adding an early (~10%) and a late (~90%)) for my most morphologically important specimens.
Tina also suggested lengthening the transition times - while it hasn't helped my specific preps, it is an excellent point.
Overall, I think you may be trying to rush your preps. Good SEM prep takes lots of time (and figuring out the most efficient prep for your particular sample often takes even longer).
Aaron
---------------------------- Aaron Barnes Dunny Lab - Dept of Microbiology University of Minnesota
} Unfortunately, my bacteria are coming out wrinkled, shriveled, and just } generally improperly dehydrated. } } I have tried several permutations of this protocol including fixing at } in the fridge for an hour, post-fixing with 1% Osmium Tetroxide for } 30min. before the dehydration process, but I haven't found anything that } has really helped. } } Does anyone have advice/experience with this sort of preparation? } } } Thanks, } Benjamin } } Login Host: 98.234.237.9 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 14, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Jul 11 21:29:34 2011 } 14, 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6C2TYmt031604 } 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 21:29:34 -0500 } 14, 25 -- Received: from localhost (localhost [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id 942B63D98F2 } 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 21:29:34 -0500 (CDT) } 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 14, 25 -- with ESMTP id bWmVb6nCI32l for {microscopy-at-microscopy.com} ; } 14, 25 -- Mon, 11 Jul 2011 21:29:34 -0500 (CDT) } 14, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 14, 25 -- by znl.com (Postfix) with ESMTPA id 03C723D98E7 } 14, 25 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jul 2011 21:29:34 -0500 (CDT) } 14, 25 -- Message-ID: {4E1BB18D.8050501-at-microscopy.com} } 14, 25 -- Date: Mon, 11 Jul 2011 21:29:33 -0500 } 14, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 14, 25 -- Reply-To: benjamin.brezler-at-gmail.com } 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 14, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 25 -- Subject: viaWWW:Bacterial Specimen Dehydration Help for SEM } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 22 -- From barnesa-at-umn.edu Tue Jul 12 15:30:50 2011 12, 22 -- Received: from vs-m.tc.umn.edu (vs-m.tc.umn.edu [134.84.135.97]) 12, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6CKUolI000742 12, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 15:30:50 -0500 12, 22 -- Received: from [160.94.104.70] (02240-ac.bsbe.umn.edu [160.94.104.70]) 12, 22 -- by vs-m.tc.umn.edu (UMN smtpd) with ESMTP 12, 22 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 15:30:50 -0500 (CDT) 12, 22 -- X-Umn-Remote-Mta: [N] 02240-ac.bsbe.umn.edu [160.94.104.70] #+LO+TS+AU+HN 12, 22 -- X-Umn-Classification: local 12, 22 -- Message-ID: {4E1CAEF9.9060505-at-umn.edu} 12, 22 -- Date: Tue, 12 Jul 2011 15:30:49 -0500 12, 22 -- From: Aaron Barnes {barnesa-at-umn.edu} 12, 22 -- Reply-To: barnesa-at-umn.edu 12, 22 -- Organization: University of Minnesota 12, 22 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 12, 22 -- MIME-Version: 1.0 12, 22 -- To: Microscopy-at-microscopy.com 12, 22 -- Subject: Re: Bacterial Specimen Dehydration Help for SEM 12, 22 -- References: {201107120238.p6C2cPLB009790-at-ns.microscopy.com} 12, 22 -- In-Reply-To: {201107120238.p6C2cPLB009790-at-ns.microscopy.com} 12, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 22 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Benjamin Bacteria are known to be tough creatures so it is difficult to comment on the difficulties you are facing in preparing them for SEM Especially if we don't see any pictures with your comments describing the problem you see. Of course in SEM anything can go wrong but in your case, speaking 'sans voir', it could be a layer of dirt covering the microbes and their fine details. Which means that you have to do something before fixation or at least before dehydration.. With best regards yorgos
----- Original Message ----- X-from: {microscopylistserver-noreply-at-microscopy.com} To: {eikonika-at-otenet.gr} Sent: Tuesday, July 12, 2011 5:36 AM
Dear all,
The A*STAR Institute of Medical Biology in Singapore has a position for an Electron Microscopy Officer available.
Full details for the job are here: http://sg.dimension.jobsdb.com/career/ad.asp?AC=ASTAR&EC=001&JobID=5013
Brief details: Electron Microscopy Officer
The IMB Microscopy Unit (IMU) is a core technology platform of IMB whose mission is to provide optical imaging and image analysis tools with expertise to facilitate research in molecular, cell and developmental biology. The recent purchase of both a scanning electron microscope (SEM) and a transmission electron microscope (TEM) along with the associated sample preparation equipment will see the establishment of the Electron Microscopy Suite and will extend the techniques available in IMU to cover both optical and electron microscopy.
Duties and responsibilities:
The successful candidate will be involved in all aspects of the operation of the IMB Electron Microcopy Suite. This will include: · Carrying out sample preparation and electron microscopy for researchers · Training, advising and supporting users in the application of electron microscopy techniques to biomedical research; from sample preparation, through image acquisition, to image processing and analysis · Developing collaborative research projects with research staff · Liaising with the manufacturers for repairs, upgrades and application support · Organising international workshops, courses and conferences · Trouble-shooting, providing basic maintenance and upkeep of microscope systems · Performing other administrative duties
Regards, Graham -- Dr Graham Wright Microscopy Unit Manager
Institute of Medical Biology 8A Biomedical Grove, #06-06 Immunos, Singapore 138648 W: http://www.imb.a-star.edu.sg/imu/
==============================Original Headers============================== 11, 26 -- From graham.d.wright-at-googlemail.com Wed Jul 13 01:26:39 2011 11, 26 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6D6Qdtr007392 11, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jul 2011 01:26:39 -0500 11, 26 -- Received: by vws4 with SMTP id 4so5125883vws.0 11, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jul 2011 23:26:39 -0700 (PDT) 11, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 26 -- d=googlemail.com; s=gamma; 11, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 11, 26 -- :content-transfer-encoding; 11, 26 -- bh=H3LLwCgiUjrGMggAdRXqApXZ2DZr6fRzHcvtQhorvRw=; 11, 26 -- b=X7CvTjRVD+QgncaveXH7v46ds89+rvDiTNGbRNZ8iG6a3RJcLSNdWem2XYk1Hp3RX8 11, 26 -- Gmu71Uzbjl7cdQOlGC3OwD8nEuYIxDVR3SqimAdYf7PPer44tgmk6PTgYkYWNqb1q3rd 11, 26 -- Qtk877qV2/APci4unp84/kIWR1eyzWYG5vbYg= 11, 26 -- MIME-Version: 1.0 11, 26 -- Received: by 10.220.5.11 with SMTP id 11mr202689vct.268.1310538397370; Tue, 12 11, 26 -- Jul 2011 23:26:37 -0700 (PDT) 11, 26 -- Received: by 10.220.162.193 with HTTP; Tue, 12 Jul 2011 23:26:37 -0700 (PDT) 11, 26 -- Date: Wed, 13 Jul 2011 14:26:37 +0800 11, 26 -- Message-ID: {CAJa2-MuJOBTK5ueh-WSE8uTzmf4cfrThNrS4Bh67RUeypHvZ=A-at-mail.gmail.com} 11, 26 -- Subject: EM - job advert, Electron Microscopy Officer 11, 26 -- From: Graham Wright {graham.d.wright-at-googlemail.com} 11, 26 -- To: Microscopy-at-microscopy.com 11, 26 -- Content-Type: text/plain; charset=ISO-8859-1 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6D6Qdtr007392 ==============================End of - Headers==============================
We have several pieces of excess equipment that will be sent to surplus/salvage within the next couple of weeks. There might be components, such as printed circuit boards, that could be useful. Please let me know by Friday, July 22, if you are interested. No guarantee that any are still functional. These items are free, but packing and shipping costs are the responsibility of the receiver.
Available: EDS, consoles only: Tracor Northern 5500 EDAX 9800 Plus Link eXL II LogE photographic printer
--John Chandler EM Lab Colorado School of Mines Golden, Colorado jpchandl-at-mines.edu
==============================Original Headers============================== 5, 22 -- From jpchandl-at-mines.edu Wed Jul 13 12:02:58 2011 5, 22 -- Received: from exch-cas2.adit.mines.edu (exch-cas2.Mines.EDU [138.67.132.205]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6DH2vYL001334 5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jul 2011 12:02:58 -0500 5, 22 -- Received: from exch-mb.adit.mines.edu ([169.254.2.217]) by exch-cas2 5, 22 -- ([138.67.138.69]) with mapi; Wed, 13 Jul 2011 11:02:57 -0600 5, 22 -- From: John Chandler {jpchandl-at-mines.edu} 5, 22 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 22 -- Date: Wed, 13 Jul 2011 11:02:06 -0600 5, 22 -- Subject: EDS and photography surplus equipment available 5, 22 -- Thread-Topic: EDS and photography surplus equipment available 5, 22 -- Thread-Index: AcxBfrYwX/FtICkwQb+hnpzbp3v7kA== 5, 22 -- Message-ID: {CE11C7F3-4FC7-4E08-A513-BC4A892039F0-at-mines.edu} 5, 22 -- Accept-Language: en-US 5, 22 -- Content-Language: en-US 5, 22 -- X-MS-Has-Attach: 5, 22 -- X-MS-TNEF-Correlator: 5, 22 -- acceptlanguage: en-US 5, 22 -- Content-Type: text/plain; charset="us-ascii" 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6DH2vYL001334 ==============================End of - Headers==============================
The little arm or finger that holds a microscope slide in place on my XY stage no longer pops back into place. I assume there is some spring inside that has tired out (I know how it feels....). Has anyone ever repaired one of these? This is on a Zeiss Axiophot but the design seems similar to other brands. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both junhe1970-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: junhe1970-at-yahoo.com Name: Jun
Organization: GCS
Title-Subject: [Filtered] CDSEM service needed
Message: We are a foundry based in Los Angeles area looking for comercial CD SEM servcie nearby. Most of our samples are 4 inch wafers. We have our own Hitachi FSEM in house but need a sencond source when it is down.
Thanks for all the replies, I didn't think there would be thast much interest in these old but reliable beasties.
Paula :-)
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 6, 26 -- From patpxs-at-gmail.com Wed Jul 13 17:52:13 2011 6, 26 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6DMqCTZ016549 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jul 2011 17:52:12 -0500 6, 26 -- Received: by iwn8 with SMTP id 8so7002338iwn.0 6, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jul 2011 15:52:12 -0700 (PDT) 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=gamma; 6, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 6, 26 -- :content-transfer-encoding; 6, 26 -- bh=D8Hm9yvZ34s6zYmMEYiJs9Mzxfgy4l44P445MQ4af+8=; 6, 26 -- b=MPScpULIP91xgm9IP1UD2tWMiwmCY/c9ZpZ6NzhtPTN6RSRrShYe8vfCz4MGLHm3lU 6, 26 -- Zsxsc2o0/clKAp5wCLGVw822p+7hZiJDTa0ZoVFgOVc6kXv9Z9g+runm658SXrfBGcn6 6, 26 -- kE9hBho5bDynR0jIkAy3FBLKymFW8ymNeiz9A= 6, 26 -- MIME-Version: 1.0 6, 26 -- Received: by 10.42.1.11 with SMTP id 11mr1023563ice.471.1310597531939; Wed, 13 6, 26 -- Jul 2011 15:52:11 -0700 (PDT) 6, 26 -- Received: by 10.42.222.5 with HTTP; Wed, 13 Jul 2011 15:52:11 -0700 (PDT) 6, 26 -- Date: Wed, 13 Jul 2011 18:52:11 -0400 6, 26 -- Message-ID: {CAPSjddbh7JQ6_fLdi_ysZ3HKU6UBkgxrBFwqjXUsmES3_dEMPw-at-mail.gmail.com} 6, 26 -- Subject: MT-2s have a new home 6, 26 -- From: Paula Sicurello {patpxs-at-gmail.com} 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6DMqCTZ016549 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rfoley-at-uab.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I used to have a company come around and clean my light microscopes about once per year. Unfortunately, they went out of business and my microscopes are REALLY dirty! Do any of you know companies that clean microscopes? Alternatively - what methods do you use to clean your lenses and digital camera connections? I'm looking for specifics as I know the lenses are quite delicate.
If it helps - I've got all Zeiss optical microscopes (5 or 6 different models).
This PDF is useful: http://www.zeiss.com/industry/general_clean_microscope.pdf
We use the recommended 85% hexane 15% isopropanol cleaning solvent and it works well.
Ben
-- Research Support Manager MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271866 {http://mrcanu.pharm.ox.ac.uk/}
On 14/07/2011 14:53, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Robin, IMHO, do it yourself is fine for a critically dirty bit that you have to clean, but there is nothing like having a professional work over your scope from lamp to camera. If you are in the New England area I can recommend Accu-Tech Optical" {service-at-accutechoptical.com} . I have had great service from them (based I think in Vermont). I don't recognize "uab" so maybe you are in Oregon. But fyi. Tobias
} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both rfoley-at-uab.edu as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: rfoley-at-uab.edu Name: Robin Foley } } Organization: UAB } } Title-Subject: [Filtered] Cleaning light microscope lenses } } Message: All, } } I used to have a company come around and clean my light microscopes } about once per year. Unfortunately, they went out of business and my } microscopes are REALLY dirty! } Do any of you know companies that clean microscopes? } Alternatively - what methods do you use to clean your lenses and digital } camera connections? I'm looking for specifics as I know the lenses are } quite delicate. } } If it helps - I've got all Zeiss optical microscopes (5 or 6 different } models). } } Thanks! } } Robin Foley } } Login Host: 138.26.80.193 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Jul } 14 08:45:45 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id p6EDjjNR030732 } 12, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 } 08:45:45 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id A84933DB754 } 12, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 } 08:45:45 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 12, 25 -- with ESMTP id ZAftRPToe-f4 for {microscopy-at-microscopy.com} ; } 12, 25 -- Thu, 14 Jul 2011 08:45:43 -0500 (CDT) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 0FD743DB749 } 12, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 } 08:45:43 -0500 (CDT) } 12, 25 -- Message-ID: {4E1EF307.9050504-at-microscopy.com} } 12, 25 -- Date: Thu, 14 Jul 2011 08:45:43 -0500 } 12, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: rfoley-at-uab.edu } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X } 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW:Cleaning light microscope lenses } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
Hello Everyone, We're having a little trouble with our Edwards Sputter Coater S150B. It seems it doesn't deposit metal, specifically gold, on samples. We purge with argon and we believe we have the voltages set correctly. Does anyone know of anyone who services these instruments? I'm concern that we're not getting the voltages we thing we are getting.
any suggestions would be welcome!
Thanks!!!
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 8, 25 -- From frank_karl-at-ardl.com Thu Jul 14 11:27:03 2011 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6EGR3Vr017042 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 11:27:03 -0500 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 14 8, 25 -- Jul 2011 12:27:03 -0400 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 8, 25 -- Date: Thu, 14 Jul 2011 12:27:02 -0400 8, 25 -- Subject: Sputtering sputter coaters 8, 25 -- Thread-Topic: Sputtering sputter coaters 8, 25 -- Thread-Index: AcxCQtxGwdHXS/dzR0+MJz9hMwRe7g== 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077D7649557-at-exchange2k7.ad.ardl.com} 8, 25 -- Accept-Language: en-US 8, 25 -- Content-Language: en-US 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- acceptlanguage: en-US 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6EGR3Vr017042 ==============================End of - Headers==============================
Hello- I'd like to know if anyone has attempted to: 1) successfully clean the oil off of a dirty mirror without ruining it 2) drilled out a larger hole through the mirror to get a wider field of view Please let me know if you have tried these things, even if it didn't work. Thanks, Dan Ruscitto
-- Dan Ruscitto, Ph.D.
Laboratory Manager 1W13 Jonsson-Rowland Science Center Earth& Environmental Sciences Rensselaer Polytechnic Institute 110 8th St. Troy NY 12180 Ph: 518-276-2372 Fax: 518-276-2012
==============================Original Headers============================== 4, 23 -- From ruscid2-at-rpi.edu Thu Jul 14 11:35:19 2011 4, 23 -- Received: from smtp5.server.rpi.edu (smtp5.server.rpi.edu [128.113.2.225]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6EGZIvw031111 4, 23 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 11:35:19 -0500 4, 23 -- Received: from [128.113.7.89] (rotameter-28.dynamic.rpi.edu [128.113.7.89]) 4, 23 -- (authenticated bits=0) 4, 23 -- by smtp5.server.rpi.edu (8.13.1/8.13.1) with ESMTP id p6EGZI3r001288 4, 23 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=NO) 4, 23 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 12:35:18 -0400 4, 23 -- Message-ID: {4E1F1AC4.5070403-at-rpi.edu} 4, 23 -- Date: Thu, 14 Jul 2011 12:35:16 -0400 4, 23 -- From: Dan Ruscitto {ruscid2-at-rpi.edu} 4, 23 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 6.1; en-US; rv:1.9.2.18) Gecko/20110616 Lightning/1.0b2 Thunderbird/3.1.11 4, 23 -- MIME-Version: 1.0 4, 23 -- To: microscopy-at-microscopy.com 4, 23 -- Subject: Gatan MonoCL3 Mirrors 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- X-Bayes-Prob: 0.0001 (Score 0) 4, 23 -- X-RPI-SA-Score: 1.40 (*) [Hold at 12.00] RATWARE_GECKO_BUILD,22490(-25) 4, 23 -- X-CanItPRO-Stream: outgoing 4, 23 -- X-Canit-Stats-ID: Bayes signature not available 4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.113.2.225 ==============================End of - Headers==============================
I routinely clean dielectric-coated front surface mirrors in positioning interferometers by blowing dust off and solvent/blow dry cleaning with airbrush. This method may work for metal front-surface mirrors, but I never tried it.
Instead of drilling larger hole in existing mirror and risking ruining the instrument, I'd suggest making a replacement with the desired hole and substituting it for existing. Higher cost, but you have a solid fallback plan in case something goes wrong.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 7/14/2011 12:36 PM, ruscid2-at-rpi.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello- } I'd like to know if anyone has attempted to: } 1) successfully clean the oil off of a dirty mirror without ruining it } 2) drilled out a larger hole through the mirror to get a wider field of } view } Please let me know if you have tried these things, even if it didn't work. } Thanks, } Dan Ruscitto }
We have in our posession a box of 7 really old Field Emission tips, circa 1986. Some have the American Optical Corporation label on them. Some have the FEI label on them.
The FEI labels also have this written on them, by hand:
The AO tips have nothing written on them to indicate how old they are but they are probably the same vintage if not older. Shoot, when did AO go out of business?
I'm not even sure what type of EM can use these.
If you are a brave soul and wants to give them a try, let me know.
You pay for shipping, etc. These are "As-Is".
Paula :-)
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 11, 26 -- From patpxs-at-gmail.com Thu Jul 14 15:29:27 2011 11, 26 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6EKTQwD004485 11, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 15:29:27 -0500 11, 26 -- Received: by iyl8 with SMTP id 8so652022iyl.0 11, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 13:29:26 -0700 (PDT) 11, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 26 -- d=gmail.com; s=gamma; 11, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 11, 26 -- :content-transfer-encoding; 11, 26 -- bh=qTC0ryalkMnM4LpDoihEDYF/aOiWIBVpamvetbBTA2Y=; 11, 26 -- b=J4tzQYJrNHXqHTIQDzgE6sDwFIJobfEBxr9pdVVW9N4hjQROKzB5DUl4a8n60J6NlP 11, 26 -- Kr+jINWGhbEQXZ09gGBwzPQ2vpLtKXqASo87cKZQc7e48abrTo+TnQ2rhWV0ve4AZDDJ 11, 26 -- AwkrZ0f+eRKlfWAbCmMHuGsPY+5cYh/sRWtPg= 11, 26 -- MIME-Version: 1.0 11, 26 -- Received: by 10.42.246.134 with SMTP id ly6mr3064637icb.136.1310675366140; 11, 26 -- Thu, 14 Jul 2011 13:29:26 -0700 (PDT) 11, 26 -- Received: by 10.42.222.5 with HTTP; Thu, 14 Jul 2011 13:29:26 -0700 (PDT) 11, 26 -- Date: Thu, 14 Jul 2011 16:29:26 -0400 11, 26 -- Message-ID: {CAPSjddaje_eVsa6p3trpxTtiLNnwA7=BYCFingG5317tZMDDLw-at-mail.gmail.com} 11, 26 -- Subject: Field Emission Tips Free 11, 26 -- From: Paula Sicurello {patpxs-at-gmail.com} 11, 26 -- To: Microscopy-at-microscopy.com 11, 26 -- Content-Type: text/plain; charset=ISO-8859-1 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6EKTQwD004485 ==============================End of - Headers==============================
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6ELQMrR021897 for {MicroscopyListserverArchive2-at-microscopy.com} ; Thu, 14 Jul 2011 16:26:22 -0500 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id p6ELQMJG021895; Thu, 14 Jul 2011 16:26:22 -0500
Hello Fellow Microscopists,
I am looking to obtain a Balzers Electron Beam Gun HV power supply designated EVM 052A. My hope is that a few of you may have a moth-balled freeze fracture unit or evaporator that used this controller for the electron beam guns. If you have one of these and would be willing to part with it, would you please contact me to discuss the details? I'll try to make the exchange as easy as possible for you.
Many thanks,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3101 S.W. Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Cell: 503-819-3600
==============================Original Headers============================== 7, 31 -- From DRK-at-shcc.org Thu Jul 14 16:26:22 2011 7, 31 -- Received: from mail131.messagelabs.com (mail131.messagelabs.com [216.82.242.99]) 7, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6ELQLnH021892 7, 31 -- for {Microscopy-at-Microscopy.Com} ; Thu, 14 Jul 2011 16:26:21 -0500 7, 31 -- X-VirusChecked: Checked 7, 31 -- X-Env-Sender: DRK-at-shcc.org 7, 31 -- X-Msg-Ref: server-14.tower-131.messagelabs.com!1310678764!30636063!32 7, 31 -- X-StarScan-Version: 6.2.17; banners=-,-,- 7, 31 -- X-Originating-IP: [216.166.12.72] 7, 31 -- Received: (qmail 29448 invoked from network); 14 Jul 2011 21:26:20 -0000 7, 31 -- Received: from out001.collaborationhost.net (HELO out001.collaborationhost.net) (216.166.12.72) 7, 31 -- by server-14.tower-131.messagelabs.com with RC4-SHA encrypted SMTP; 14 Jul 2011 21:26:20 -0000 7, 31 -- Received: from AUSP01VMBX30.collaborationhost.net ([10.2.12.44]) by 7, 31 -- AUSP01MHUB50.collaborationhost.net ([10.2.10.3]) with mapi; Thu, 14 Jul 2011 7, 31 -- 16:26:11 -0500 7, 31 -- From: Doug Keene {DRK-at-shcc.org} 7, 31 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 7, 31 -- Date: Thu, 14 Jul 2011 16:28:56 -0500 7, 31 -- Subject: Balzers Control Unit EVM 052 for electron beam gun 7, 31 -- Thread-Topic: Balzers Control Unit EVM 052 for electron beam gun 7, 31 -- Thread-Index: AcxCbQlIwF6kpGmERym8aZBVaJvmAA== 7, 31 -- Message-ID: {1DDBEFFEDA3EED4B8817469E593164AA137993404D-at-AUSP01VMBX30.collaborationhost.net} 7, 31 -- Accept-Language: en-US 7, 31 -- Content-Language: en-US 7, 31 -- X-MS-Has-Attach: 7, 31 -- X-MS-TNEF-Correlator: 7, 31 -- acceptlanguage: en-US 7, 31 -- Content-Type: text/plain; charset="us-ascii" 7, 31 -- MIME-Version: 1.0 7, 31 -- Content-Transfer-Encoding: 8bit 7, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6ELQLnH021892 ==============================End of - Headers==============================
Hello Frank, We had similar trouble with our old Polaron sputter-coater. The problem was in the HT cable. The central lead wire was broken. Fortunately, the cable was available as spare part.
I hope it might help you.
Best regards Oldrich
-- Oldrich Benada Institute of Microbiology ASCR, v.v.i. Videnska 1083 142 20 Prague 4
On Thursday 14 of July 2011 18:29:12 you wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } Hello Everyone, } We're having a little trouble with our Edwards Sputter Coater S150B. It } seems it doesn't deposit metal, specifically gold, on samples. We purge } with argon and we believe we have the voltages set correctly. Does anyone } know of anyone who services these instruments? I'm concern that we're not } getting the voltages we thing we are getting. } } any suggestions would be welcome! } } Thanks!!! } } Frank Karl } Microscopist } ARDL } 2887 Gilchrist Road } Akron, Ohio 44305 } 330-794-6600 } } } } ________________________________ } This email and any of its attachments may contain confidential information } intended only for the use of the addressee(s). If the reader of this email } is not the intended recipient or the employee or agent responsible for } delivering it to the intended recipient, you are hereby notified that any } dissemination or copying of this email is strictly prohibited. If you have } received this email in error, please notify us by return email at } info-at-ardl.com, permanently delete the email, and destroy any printouts. If } this email contains test data and/or draft reports, you are hereby } notified that only a signed original test report will contain official } results, a copy of which resides in the project folder located at ARDL, } Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } ==============================Original } Headers============================== 8, 25 -- From frank_karl-at-ardl.com } Thu Jul 14 11:27:03 2011 } 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p6EGR3Vr017042 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jul 2011 } 11:27:03 -0500 8, 25 -- Received: from exchange2k7.ad.ardl.com } ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com } ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 14 8, 25 -- Jul 2011 } 12:27:03 -0400 } 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} } 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} } 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} } 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} } 8, 25 -- Date: Thu, 14 Jul 2011 12:27:02 -0400 } 8, 25 -- Subject: Sputtering sputter coaters } 8, 25 -- Thread-Topic: Sputtering sputter coaters } 8, 25 -- Thread-Index: AcxCQtxGwdHXS/dzR0+MJz9hMwRe7g== } 8, 25 -- Message-ID: } {DB672743AFB6A64B9EB5CAC80AF15077D7649557-at-exchange2k7.ad.ardl.com} 8, 25 } -- Accept-Language: en-US } 8, 25 -- Content-Language: en-US } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- acceptlanguage: en-US } 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p6EGR3Vr017042 ==============================End of - } Headers==============================
High voltage connections are quite probable cause. For my new sputter coater I needed to tighten screw to restore HT connection.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz] } Sent: Friday, July 15, 2011 4:16 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Re: Sputtering sputter coaters } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Hello Frank, } We had similar trouble with our old Polaron sputter-coater. The problem } was in the HT cable. The } central lead wire was broken. Fortunately, the cable was available as } spare part. } } I hope it might help you. } } Best regards Oldrich } } -- } Oldrich Benada } Institute of Microbiology ASCR, v.v.i. } Videnska 1083 } 142 20 Prague 4 } } On Thursday 14 of July 2011 18:29:12 you wrote: } } --------------------------------------------------------------------- } ------ } } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help
If you have been using the sputter coater for a while it may just be that the target needs to be cleaned. I think that even a thin coat of hydrocarbons that are barely visible can impede coating. You will still see the argon plasma and normal current/vacuum readings.
Kevin
-----Original Message----- X-from: DusevichV-at-umkc.edu [mailto:DusevichV-at-umkc.edu] Sent: Friday, July 15, 2011 8:42 AM To: Nick, Kevin (LLU)
High voltage connections are quite probable cause. For my new sputter coater I needed to tighten screw to restore HT connection.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz] } Sent: Friday, July 15, 2011 4:16 AM } To: Dusevich, Vladimir } Subject: [Microscopy] Re: Sputtering sputter coaters } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Hello Frank, } We had similar trouble with our old Polaron sputter-coater. The problem } was in the HT cable. The } central lead wire was broken. Fortunately, the cable was available as } spare part. } } I hope it might help you. } } Best regards Oldrich } } -- } Oldrich Benada } Institute of Microbiology ASCR, v.v.i. } Videnska 1083 } 142 20 Prague 4 } } On Thursday 14 of July 2011 18:29:12 you wrote: } } --------------------------------------------------------------------- } ------ } } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver On-Line Help
I am having new found fun with our donated gift of a working ADDA2 on our 6301F thanks to Frank in the Albert Einstein College of Medicine. One thing we were wondering is there a com port somewhere in the inerds of a 6300 or 6400 jeol microscope that we can use to read the mag externally through the ADDA2. If anyone knows of where we might find it and on which board. I would appriciate the info. We still are not sure and Jeol is checking if this microscope has had the necessary firmware upgrade but they too are unsure of the location of the com port. So I thought I would ask the list.
Thanks in advance. John
==============================Original Headers============================== 3, 23 -- From john.mitchels-at-gmail.com Fri Jul 15 11:47:20 2011 3, 23 -- Received: from mail-pz0-f53.google.com (mail-pz0-f53.google.com [209.85.210.53]) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6FGlI7b011648 3, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jul 2011 11:47:19 -0500 3, 23 -- Received: by pzk6 with SMTP id 6so1880584pzk.26 3, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jul 2011 09:47:18 -0700 (PDT) 3, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 23 -- d=gmail.com; s=gamma; 3, 23 -- h=mime-version:date:message-id:subject:from:to:content-type; 3, 23 -- bh=nMAQJpINjbe7i/WpcY+bx/u8Foo9QI1h/1pqoRunO34=; 3, 23 -- b=f4CcvPccKBkWM5yoFjgJVENTaPugJguNNol2m4/pNrgKL12C2QVgxAY2Hf5bgzmaPo 3, 23 -- eIYwSEyuMv9RyP15R8uBJ4IYMK/6oq851WukXlsh+jdOAVFx86MngJNDY6NHCV4mJG5u 3, 23 -- 08+5jcLQapAjY4otHuPW58/rU90k8/3r6khrM= 3, 23 -- MIME-Version: 1.0 3, 23 -- Received: by 10.68.28.137 with SMTP id b9mr3724010pbh.191.1310748437878; Fri, 3, 23 -- 15 Jul 2011 09:47:17 -0700 (PDT) 3, 23 -- Received: by 10.68.41.225 with HTTP; Fri, 15 Jul 2011 09:47:17 -0700 (PDT) 3, 23 -- Date: Fri, 15 Jul 2011 17:47:17 +0100 3, 23 -- Message-ID: {CAHX7yTRrkpnbyXhwon0mxJ3OAtzU6u=EX6Q+X0w3U4GpuQnV6A-at-mail.gmail.com} 3, 23 -- Subject: Mag Com Port Jeol 6300 or 6400 series 3, 23 -- From: John Mitchels {john.mitchels-at-gmail.com} 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
I will be processing a set of insect specimens for a group of high school students. I've spent some time web-searching for SEM protocols and I haven't found many.
What is the best way to fix insects in the field. (The students will be doing this with an instructor/supervisor who will later bring the specimens to me.)
I regularly process mammalian tissue for SEM, but what is the best way to process insects?
(As part of a twelve-Saturday arts/science program, the students will be designing and producing interpretive signage for a local National Fish and Wildlife refuge. The SEM images will be incorporated into the signage.)
Thank you,
Jaci
Jaclynn Lett Senior Research Technician Research Center for Auditory and Vestibular Studies Department of Otolaryngology Washington University School of Medicine 660 S Euclid Avenue, Campus Box 8115 St. Louis, MO 63110
The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email.
==============================Original Headers============================== 12, 23 -- From LettJ-at-ent.wustl.edu Fri Jul 15 17:13:03 2011 12, 23 -- Received: from MAIL3.wusm-pcf.wustl.edu (mail3.wusm-pcf.wustl.edu [128.252.17.170]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6FMD1EX031667 12, 23 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jul 2011 17:13:02 -0500 12, 23 -- Received: from EX04.wusm-pcf.wustl.edu ([10.39.162.184]) by MAIL3.wusm-pcf.wustl.edu with Microsoft SMTPSVC(6.0.3790.4675); 12, 23 -- Fri, 15 Jul 2011 17:12:59 -0500 12, 23 -- Content-class: urn:content-classes:message 12, 23 -- MIME-Version: 1.0 12, 23 -- Content-Type: text/plain; 12, 23 -- charset="us-ascii" 12, 23 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 23 -- Subject: SEM: protocol for insects 12, 23 -- Date: Fri, 15 Jul 2011 17:12:59 -0500 12, 23 -- Message-ID: {1C8B3F4710BBDD4CA24CB5269F2B4C3901E997D6-at-EX04.wusm-pcf.wustl.edu} 12, 23 -- X-MS-Has-Attach: 12, 23 -- X-MS-TNEF-Correlator: 12, 23 -- Thread-Topic: SEM: protocol for insects 12, 23 -- Thread-Index: AcxDPFsRWm+KW90hSWevqPPfk5l5FQ== 12, 23 -- From: "Lett, Jaclynn" {LettJ-at-ent.wustl.edu} 12, 23 -- To: {microscopy-at-microscopy.com} 12, 23 -- X-OriginalArrivalTime: 15 Jul 2011 22:12:59.0466 (UTC) FILETIME=[5B3C02A0:01CC433C] 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6FMD1EX031667 ==============================End of - Headers==============================
I would like to call your attention to the following information about a series of 1-day workshops to be held at the Life Science Microscopy Facility at Purdue university. We have two FEG-SEMs with cryosystems and they are heavily used. It has turned out to be a very useful technique for many different types of hydrated samples...not just biological samples.
================================================================== Cryo SEM Workshop - August 3 - 4, 2011
Hosted by: The Life Science Microscopy Facility Purdue University
You are invited to a FREE Cryo SEM Workshop hosted by Purdue University.
This Workshop will cover basic and advanced techniques and methodology for cryo-imaging. Lectures and lab sessions are coordinated to provide an interactive educational program for all participants. Attendees are encouraged to sign up for a one day session and bring specimens for preparation and imaging using Gatan¹s Alto 2500 systems.
*Marilyn Carey is a Cryo-SEM Specialist at Gatun UK
Bring your own Cryo samples to run on Purdue University Cryo SEM Systems: NOVA nanoSEM FESEM Quanta 3D FEG
To register for the one day session please contact:
Steve Nagy - Gatan Central Regional SEM Mgr. SNagy-at-Gatan.com {mailto:SNagy-at-Gatan.com} (612) 616-9557
Hotel Accommodations: Hilton Garden Inn 356 East State Street, West Lafayette, Indiana 47906
Telephone: 765 743-2100 or www.hiltongardeninn.com {http://www.hiltongardeninn.com}
Regards, Debby
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
Below please find a description of a postdoctoral position in the areas of scanning probe and electron microscopy of energy storage materials. I will greatly appreciate if you can bring it to attention of potential candidates with experience in SPM of energy materials, PFM/nanoferroelectrics, in-situ SPM, and in-situ (S)TEM.
The Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory (ORNL) is seeking to fill a postdoctoral position in the field of scanning probe and electron microscopy of energy storage and conversion systems. The applicant will conduct research on the nanoscale functionality of energy materials, including Li-ion batteries and fuel cells. The primary experimental technique is electrochemical strain microscopy under environmental control. Research will be directed toward the correlation of macroscopic and nanoscopic parameters and their role in electrochemical functionality in current and advanced battery materials. The program will have a strong component of innovative technique development, namely the development of an in-situ electrochemical cell to be used with the scanning probe microscope. The CNMS {http://cnms.ornl.gov/} is a collaborative nanoscience user research facility established by the Office of Science, U.S. Department of Energy.
This position requires a Ph.D. in condensed matter physics, materials science, electrochemistry or a related field. The successful applicant should have a strong background in scanning probe microscopy in ambient and liquid environment or in-situ electrochemical experiments using scanning and transmission electron microscopy, synchrotron methods, or scanning probe microscopy. Familiarity with numerical data analysis and customization of control software is a plus (Labview, Matlab). Prior research work needs to be supported by a strong record of publications in peer-reviewed journals, preferably as a senior author, and presentations at scientific conferences. The applicant should be motivated, safety conscious and possess excellent oral and written communication skills. The applicant must have the ability to work in a team, interact effectively with colleagues and external collaborators. Demonstrated ability to communicate in English to an international scientific audience is essential.Applicants must have received their highest degree not more than five years prior to the date of application, and must complete all degree requirements before starting the appointment.
*Technical Questions: *For more information about this position, please contact Dr. Nina Balke (_n2b-at-ornl.gov {mailto:5nm-at-ornl.gov} _) and reference the ad number listed above in the subject line. Applications will be accepted until the position is filled.
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 12, 25 -- From sergei2-at-ornl.gov Mon Jul 18 11:40:35 2011 12, 25 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6IGeY8x012384 12, 25 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jul 2011 11:40:34 -0500 12, 25 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 12, 25 -- by emroute1.ornl.gov (PMDF V6.5-x6 #31890) 12, 25 -- with ESMTP id {0LOJ00908FNLL3-at-emroute1.ornl.gov} for 12, 25 -- microscopy-at-microscopy.com; Mon, 18 Jul 2011 12:40:33 -0400 (EDT) 12, 25 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 12, 25 -- (PMDF V6.5-x6 #31890) id {0LOJ00601FNLTQ-at-emroute1.ornl.gov} for 12, 25 -- microscopy-at-microscopy.com; Mon, 18 Jul 2011 12:40:33 -0400 (EDT) 12, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 12, 25 -- by emroute1.ornl.gov (PMDF V6.5-x6 #31890) 12, 25 -- with ESMTPS id {0LOJ00984FNK20-at-emroute1.ornl.gov} for 12, 25 -- microscopy-at-microscopy.com; Mon, 18 Jul 2011 12:40:33 -0400 (EDT) 12, 25 -- Date: Mon, 18 Jul 2011 12:40:32 -0400 12, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 12, 25 -- Subject: Position opening 12, 25 -- To: microscopy-at-microscopy.com 12, 25 -- Message-id: {4E246200.1060408-at-ornl.gov} 12, 25 -- MIME-version: 1.0 12, 25 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 12, 25 -- Content-transfer-encoding: 7bit 12, 25 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) 12, 25 -- Gecko/20110616 Lightning/1.0b2 Thunderbird/3.1.11 ==============================End of - Headers==============================
Last week I posted a "want" for a Balzers EVM052A electron beam gun controller. So far no one has come forward with a working unit, perhaps not surprising since these units were of 1980's vintage. But it did occur to me that my last message might have suggested I was looking for a donation; in fact I am willing to pay $$$ for a working unit. If you have one that you are willing to part with, please respond to me directly so that we can work out the details.
Many thanks,
Doug
Douglas R. Keene Assistant Investigator Micro-Imaging Center Shriners Hospital for Children 3101 S.W. Sam Jackson Park Road Portland, Oregon 97239 Office: 503-221-3434 Cell: 503-819-3600
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both candace_haigler-at-ncsu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] TEM Biology Position Available
Message: A postdoc or other research associate position is available at North Carolina State University, Raleigh, NC in the lab of Dr. Candace Haigler, Professor of Crop Science and Plant Biology (candace_haigler-at-ncsu.edu) as part of the Center for Lignocellulose Structure and Formation. The research will focus on advanced TEM to elucidate the structure and function of the cellulose-synthesizing complex in plant systems. The intellectually challenging research will be coordinated with other Center researchers working in the areas of molecular genetics, nanobioengineering, computational modeling, and biochemistry. Work will involve independent use of TEM, thin sectioning, immunolabeling, and sample preparation by freeze fracture, with training possible for freeze fracture for otherwise highly qualified candidates. Persons with a PhD emphasizing biological TEM or highly experienced candidates with a M.S. degree may apply at http://jobs.ncsu.edu for position number 101332, which also provides further details about the application process. NC State University is an equal opportunity/affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, national origin, religion sex, age, veteran status, disability, or sexual orientation. Individuals with disabilities desiring accommodations during the application process should contact Joyce Elias at 919-515-2648.
All, I would like to gauge the interest of the EM community in a new version of Desktop Microscopist. As many of you know, currently the accessibility of the old Mac version of DM is becoming obsolete, as I believe the last system it would operate on was OS9. Recently we have been in contact with the rights owner/creator of DM to possibly develop a new version which would run on any system (PC, Mac, Linux). It would be helpful to have an idea of who still uses DM, and if there is a desire to have it updated.
Our group still uses DM on a regular basis to solve any number of phase identification and orientation relationships in the oxidation of stainless steels, and so we are hopeful we can bring it over for a new generation of electron microscopists.
Emails responses can be sent to matthew.olszta-at-pnnl.gov
Regards, Matt Olszta
==============================Original Headers============================== 5, 24 -- From prvs=17408b823=matthew.olszta-at-pnnl.gov Tue Jul 19 10:55:00 2011 5, 24 -- Received: from emailgw03.pnl.gov (emailgw03.pnl.gov [192.101.109.31]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6JFsxDQ004455 5, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 19 Jul 2011 10:54:59 -0500 5, 24 -- Received: from emailhub01.pnl.gov ([130.20.251.61]) 5, 24 -- by emailgw03.pnl.gov with ESMTP/TLS/AES128-SHA; 19 Jul 2011 08:54:58 -0700 5, 24 -- Received: from Email04.pnl.gov ([130.20.251.63]) by emailhub01.pnl.gov 5, 24 -- ([130.20.251.61]) with mapi; Tue, 19 Jul 2011 08:54:58 -0700 5, 24 -- From: "Olszta, Matthew J" {matthew.olszta-at-pnnl.gov} 5, 24 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 24 -- Date: Tue, 19 Jul 2011 08:54:49 -0700 5, 24 -- Subject: Desktop Microscopist 5, 24 -- Thread-Topic: Desktop Microscopist 5, 24 -- Thread-Index: AcxGLDCM8cDUOqvZSZ6bJFKRak66Mg== 5, 24 -- Message-ID: {02748B1F2ABDB84EBFB546F8789ED5590183D54C77C5-at-EMAIL04.pnl.gov} 5, 24 -- Accept-Language: en-US 5, 24 -- Content-Language: en-US 5, 24 -- X-MS-Has-Attach: 5, 24 -- X-MS-TNEF-Correlator: 5, 24 -- acceptlanguage: en-US 5, 24 -- Content-Type: text/plain; charset="us-ascii" 5, 24 -- MIME-Version: 1.0 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6JFsxDQ004455 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both r.g.phillips-at-sussex.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: r.g.phillips-at-sussex.ac.uk Name: Roger Phillips
Organization: University of Sussex, School of Life Sciences
Title-Subject: [Filtered] Imaging in Biomedical Research at the University of Sussex
Message: Dear colleagues, I am Programme Convenor for the MSc in Imaging in Biomedical Research at the University of Sussex. Due to late withdrawals, we can still offer two fully funded (fees and stipend) MRC studentships for our course. I would be very grateful if you would pass information about this opportunity on to your graduated tutees. The programme includes taught and research components and covers various techniques in microscopy, medical imaging and image processing in equal parts. For further information and for application please follow the links: http://www.sussex.ac.uk/study/pg/2011/taught/1654/23871#tabs-1 http://www.sussex.ac.uk/study/pg/applying/ Please advise any interested student to contact me at R.G.Phillips-at-sussex.ac.uk Thanks for your help, Roger
Dr Roger Guy Phillips Centre for Advanced Microscopy, University of Sussex School of Life Sciences John Maynard Smith Building Falmer, Brighton & Hove BN1 9QG United Kingdom
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both guosheng.liu-at-usask.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: guosheng.liu-at-usask.ca Name: Guosheng Liu
Organization: U of S
Title-Subject: [Filtered] Ultramicrotome problem
Message: Hi, All,
Our two Reichert-Jung Ultracut microtomes are long due for tuning up or service. The worst is that the Reset Control for Specimen Advance knob doesn't work yesterday (the warning light keep flashing). Any suggestion/idea for fixing it? I noticed an message that was sent by Dorota two months ago requesting for service (see below) but no replies were posted. I got similar question: any service available for those old microtomes in north America?
Thanks a lot,
Guosheng Liu
_______________________________________________________________ Organization: Atlantic Veterinary College at UPEI
Ultramicrotome service
Hi, I am looking for a service for our Reichert-Jung Ultracut E microtome in Maritime area, Canada. Our ultramicrotomes are in need of a tune up or more. Thank you Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both garnet.martens-at-botany.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] long term effects of flooding
Message: Hello all,
We suffered a major flood in our emlab over the past weekend. A water pipe on the third floor of our building fell apart on Saturday morning and eventually flooded the entire wing. All four of our scopes got very wet and I was wondering if anyone out there has had a similar experience. The water filtered through three floors of concrete and was basically brine by the time in hit the basement. Some of our ancillary equipment is now showing signs of rust and some external parts of the scopes are showing rust as well. By the way, this is our third flood in 6 years but definitely the worst.
Hoping for the best and the scopes are resurrected what are some of the long term effects that you have discovered? How have the microscope companies dealt with extended warranties, etc. Thanks,
Garnet
-- Garnet Martens Research Manager BioImaging Facility 6270 University Blvd Vancouver, BC V6T 1Z4 CANADA
I don't know what type of scope this is, or what vintage, but I was teaching in my classroom in West Palm Beach, FL when a torrential deluge erupted, which resulted in the flooding of my classroom, mid-lecture. (Apparently, as we later found out, the downspouts were all pointing into a drainage ditch which ran into my room, which was in the corner of the building) I had a JEOL JSM-35CF in there, and although there was some rusting around the base, everything was OK, as the height of the base was two inches or so, which was below the high water line. I would imagine the true extent of the damage really depends on how high the water got, and what components came into contact with it. That being said, everything was allowed to dry off for a significant amount of time before I felt comfortable powering anything up again.
--Justin A. Kraft
On Jul 19, 2011, at 9:07 PM, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both garnet.martens-at-botany.ubc.ca as well as } the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: garnet.martens-at-botany.ubc.ca Name: Garnet Martens } } Organization: UBC Bioimaging Facility } } Title-Subject: [Filtered] long term effects of flooding } } Message: Hello all, } } We suffered a major flood in our emlab over the past weekend. A water } pipe on the third floor of our building fell apart on Saturday morning } and eventually flooded the entire wing. All four of our scopes got very } wet and I was wondering if anyone out there has had a similar } experience. The water filtered through three floors of concrete and was } basically brine by the time in hit the basement. Some of our ancillary } equipment is now showing signs of rust and some external parts of the } scopes are showing rust as well. By the way, this is our third flood in } 6 years but definitely the worst. } } Hoping for the best and the scopes are resurrected what are some of the } long term effects that you have discovered? How have the microscope } companies dealt with extended warranties, etc. } Thanks, } } Garnet } } -- } Garnet Martens } Research Manager } BioImaging Facility } 6270 University Blvd } Vancouver, BC } V6T 1Z4 } CANADA } } garnet.martens-at-botany.ubc.ca } www.emlab.ubc.ca } 604-822-3354 } } } Login Host: 137.82.85.214 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 14, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 19 20:02:19 2011 } 14, 25 -- Received: from znl.com ([206.69.208.20]) } 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6K12JqL024151 } 14, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2011 20:02:19 -0500 } 14, 25 -- Received: from localhost (localhost [127.0.0.1]) } 14, 25 -- by znl.com (Postfix) with ESMTP id C72883DFC65 } 14, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2011 20:02:19 -0500 (CDT) } 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 14, 25 -- Received: from znl.com ([127.0.0.1]) } 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 14, 25 -- with ESMTP id yb3wmURFbTyk for {microscopy-at-microscopy.com} ; } 14, 25 -- Tue, 19 Jul 2011 20:02:16 -0500 (CDT) } 14, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 14, 25 -- by znl.com (Postfix) with ESMTPA id ECA313DFC5A } 14, 25 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2011 20:02:16 -0500 (CDT) } 14, 25 -- Message-ID: {4E262918.5090104-at-microscopy.com} } 14, 25 -- Date: Tue, 19 Jul 2011 20:02:16 -0500 } 14, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 14, 25 -- Reply-To: garnet.martens-at-botany.ubc.ca } 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 14, 25 -- MIME-Version: 1.0 } 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 25 -- Subject: viaWWW:long term effects of flooding } 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 14, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 33 -- From kraftpiano-at-gmail.com Tue Jul 19 20:12:38 2011 8, 33 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 8, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6K1Cb1T027388 8, 33 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2011 20:12:37 -0500 8, 33 -- Received: by yxj17 with SMTP id 17so2310338yxj.0 8, 33 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jul 2011 18:12:37 -0700 (PDT) 8, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 33 -- d=gmail.com; s=gamma; 8, 33 -- h=subject:mime-version:content-type:from:in-reply-to:date:cc 8, 33 -- :content-transfer-encoding:message-id:references:to:x-mailer; 8, 33 -- bh=ncXgVwIUWyekvOyP6oLoaaWkOxQdKTEzbGYLaFczPPY=; 8, 33 -- b=Eh9Jt7AN70wOucK9p1kcgFmGsT45F+bKcTWcT9N5S4vp14UNacD4r0tBpf2MvQXC2M 8, 33 -- i3b3SGpxKt3jGWRL4jf3M34meuFCbfkj95mkkW7vDYfAOj30NHt4DlCfS1X5d0iwYVbs 8, 33 -- BOWoXDg3aHk08d/EdnQolXsZ/HoYCYFyHZ8do= 8, 33 -- Received: by 10.236.75.228 with SMTP id z64mr9855640yhd.211.1311124355988; 8, 33 -- Tue, 19 Jul 2011 18:12:35 -0700 (PDT) 8, 33 -- Received: from [10.101.1.3] (c-66-229-197-89.hsd1.fl.comcast.net [66.229.197.89]) 8, 33 -- by mx.google.com with ESMTPS id v4sm384849yhm.20.2011.07.19.18.12.34 8, 33 -- (version=TLSv1/SSLv3 cipher=OTHER); 8, 33 -- Tue, 19 Jul 2011 18:12:34 -0700 (PDT) 8, 33 -- Subject: Re: [Microscopy] viaWWW:long term effects of flooding 8, 33 -- Mime-Version: 1.0 (Apple Message framework v1084) 8, 33 -- Content-Type: text/plain; charset=us-ascii 8, 33 -- From: Justin Kraft {kraftpiano-at-gmail.com} 8, 33 -- In-Reply-To: {201107200107.p6K17qIH011722-at-ns.microscopy.com} 8, 33 -- Date: Tue, 19 Jul 2011 21:12:33 -0400 8, 33 -- Cc: garnet.martens-at-botany.ubc.ca 8, 33 -- Message-Id: {D940A6A9-16F7-4314-B8CC-93EAE66EEC5F-at-gmail.com} 8, 33 -- References: {201107200107.p6K17qIH011722-at-ns.microscopy.com} 8, 33 -- To: microscopy-at-microscopy.com 8, 33 -- X-Mailer: Apple Mail (2.1084) 8, 33 -- Content-Transfer-Encoding: 8bit 8, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6K1Cb1T027388 ==============================End of - Headers==============================
Those of you who have had your instruments flooded recently may be interested in products called"Vapor Phase Corrosion Inhibitors". I first encountered this stuff while in the Navy, working in the Corrosion Group at the Naval Research Laboratory back during WWII. These are compounds that give off a vapor that adsorbs onto metal surfaces and prevents corrosion from occurring. The amount adsorbed is of the order of a monomolecular layer - you"ll never know it's there. We found we could ship steel rocket cases overseas with water sloshing around in them, but no corrosion would occur as long as a VPI was present. This leads me to think that if you put some VPI inside the cabinets of the instruments that have been flooded it might well prevent them from rusting.
Incidentally, if you buy a fancy, expensive tool, like a micrometer caliper, you will often find a piece of yellow paper in the case with it. This paper has been treated with a VPI and is present to keep the tool from rusting during shipment and while on the dealers shelf.
The VPI we used back in the 40s was manufactured by the Shell Chemical Co. If you do a Google search for Vapor Phase Corrosion Inhibitors you will find numerous references. From these it appears that the Cortec Co. is now a major manufacturer of them. Perhaps it would be worthwhile to get in contact with them and see if they might suggest something that would keep your instruments from rusting.. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
Dear listers, I will be moving shortly, and am trying to downsize some of the random stuff I have hanging around that I do not need. In that vein, I have a Link eXL system that is fully functional (No detector) complete with software install disks and a bunch of manuals. I also have a complete set of spare parts for it to keep it running. The software is node-locked to the CPU, so those will have to go together, but if there is anyone out there still maintaining one of these systems, this is a great opportunity to get replacement parts and spares for the cost of shipping!
Unfortunately, if it doesn't get claimed before I leave for M&M, it will get tossed.
--Justin A. Kraft
==============================Original Headers============================== 3, 30 -- From kraftpiano-at-gmail.com Wed Jul 20 07:18:31 2011 3, 30 -- Received: from mail-gx0-f169.google.com (mail-gx0-f169.google.com [209.85.161.169]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KCIUL3014425 3, 30 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:18:31 -0500 3, 30 -- Received: by gxk23 with SMTP id 23so75697gxk.0 3, 30 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 05:18:30 -0700 (PDT) 3, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 30 -- d=gmail.com; s=gamma; 3, 30 -- h=from:content-type:content-transfer-encoding:subject:date:message-id 3, 30 -- :to:mime-version:x-mailer; 3, 30 -- bh=1iF+hL11jr+TU05u6O8+GjoP3+OSwfMxecsTT6oa684=; 3, 30 -- b=VxPsprqQ3izAPJm3QMt5VQEq/JCx3E0slN0m5iJ2YfjWEz0trC/XhUY19LBLJE8RZp 3, 30 -- LwoW9MssgJUiQ4B/Z4cY7zAQZyG9g0fbs/gC4A+Gr2QXXokMtlgQSgBprQpMOOPAatHE 3, 30 -- rdR6b//ZvwQ4HIH0AmgKPgqKRU1Bwyw8NyIlU= 3, 30 -- Received: by 10.236.195.69 with SMTP id o45mr11464471yhn.460.1311164309933; 3, 30 -- Wed, 20 Jul 2011 05:18:29 -0700 (PDT) 3, 30 -- Received: from [10.101.1.3] (c-66-229-197-89.hsd1.fl.comcast.net [66.229.197.89]) 3, 30 -- by mx.google.com with ESMTPS id c69sm97523yhm.29.2011.07.20.05.18.28 3, 30 -- (version=TLSv1/SSLv3 cipher=OTHER); 3, 30 -- Wed, 20 Jul 2011 05:18:28 -0700 (PDT) 3, 30 -- From: Justin Kraft {kraftpiano-at-gmail.com} 3, 30 -- Content-Type: text/plain; charset=us-ascii 3, 30 -- Subject: Link eXL system to a good home. 3, 30 -- Date: Wed, 20 Jul 2011 08:18:27 -0400 3, 30 -- Message-Id: {B19B25E8-125A-44A2-AB1C-C8A529B05CDB-at-gmail.com} 3, 30 -- To: microscopy-at-microscopy.com 3, 30 -- Mime-Version: 1.0 (Apple Message framework v1084) 3, 30 -- X-Mailer: Apple Mail (2.1084) 3, 30 -- Content-Transfer-Encoding: 8bit 3, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6KCIUL3014425 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bengu-at-fen.bilkent.edu.tr as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Need help with Ion Pump problem on a EVO40
Message: Hi All,
We are currently experiencing a problem with our Carl-Zeiss EVO 40 SEM. We have fitted this SEM with a LaB6 filament, and the gun area is pumped with an ion pump. We occasionally (every 3-4 months) bake the gun area to reach ~ 2-3 x 10(-7) mbar vacuum levels as a req for the LaB6 filament operation. During the last bake, we found that the ion pump stopped working during the cool down stage while the final vacuum level in the system was good around 10(-7) mbar levels. However, since then we were not able to keep the ion-pump working. When we turn the ion pump on, it slowly dies away. That is, the reading of the vacuum level in the gun area is thru the ion current (there is not an extra ion gauge in the system), and the vacuum level starts at 10(-8)mbars and within 5 mins goes down to 10(-11)mbars. Finally, the ion pump shuts down. This pattern repeated after several restarts of the system. I tried gently tapping the ion pump with a plastic handle of a screwdriver, which kept vacuum level around 10(-8)'s and the pump running, but I think that is not a viable option for running any SEM with decent resolution. :)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] service ultramicrotome
Message: Hi, I posted a message asking about ultramicrotomes and I also sent e-mails to EM labs in my area. Depending on what model of Reichetr-Jung you have there might or might not be a service available. I got a contact for Leica Microsystems and they agreed to service it. I have Ultracut E and apparantly there is no service available at all. Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both richard.ross-at-allisontransmission.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: richard.ross-at-allisontransmission.com Name: Richard Ross
Organization: Allison Transmission Inc. Title-Subject: [Microscopy] Long term effects of flooding
Message: Long term effects can't be good. In general, corrosion will be promoted. If electrical ciruitry is powered up while wet, electricity will aggressively accelerate the corrosion. Definitely not good. Sounds like your facilities people are about as concerned as our were. For years we had an ancient air handler above our SEM that leaked steam condensate in the winter and A/C condensate in the summer, not to mention roof leaks. I finally built an ugly tent of reinforced polyethylene sheeting over the scope. Finally, our area was remodeled and the leaking air handler disappeared. Now its just the occasional roof leak. Login Host: 12.53.132.5 ---------------------------------------------------------------------------
==============================Original Headers============================== 6, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jul 20 07:21:13 2011 6, 25 -- Received: from znl.com ([206.69.208.20]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KCLCxD018414 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:21:12 -0500 6, 25 -- Received: from localhost (localhost [127.0.0.1]) 6, 25 -- by znl.com (Postfix) with ESMTP id EC0CF3E022D 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:21:12 -0500 (CDT) 6, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 6, 25 -- Received: from znl.com ([127.0.0.1]) 6, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 6, 25 -- with ESMTP id faDUig2xW44N for {microscopy-at-microscopy.com} ; 6, 25 -- Wed, 20 Jul 2011 07:21:12 -0500 (CDT) 6, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 6, 25 -- by znl.com (Postfix) with ESMTPA id A82BC3E0222 6, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:21:12 -0500 (CDT) 6, 25 -- Message-ID: {4E26C838.80500-at-microscopy.com} 6, 25 -- Date: Wed, 20 Jul 2011 07:21:12 -0500 6, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 6, 25 -- Reply-To: richard.ross-at-allisontransmission.com 6, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 25 -- MIME-Version: 1.0 6, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 6, 25 -- Subject: viaWWW:Long term effects of flooding 6, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We badly need your help because at the moment our Jeol JCXA 733 (Superprobe) EPMA instrument at the Institute for Geochemical Research, Hungary cannot be controlled by its original computer interface for some time. It had originally been equipped with a DEC PDP-11 minicomputer but in 1996 we replaced the PDP with a PC [Z.Valkai, L.Varga: PC interface for an old-fashioned EPMA, proc. of EMAS'96, 2nd Regional Workshop on Electron Probe Microanal. of Materials Today - Practical Aspects, 19-22 May 1996, Balatonfüred, Hungary, p.71-72 (poster session)]. Our solution was based on a special ISA card developed by my colleague. This card is connected to the UNIBUS lines so that the PC could play the exact role of the PDP. After working for some 15 years this system is out of order now. The Superprobe can still be controlled manually when the three state switch on the operation console is in LOCAL mode. My colleague has investigated the PC card and fixed some errors of the card. However, the system still does'nt work under computer control. We suspect that something went wrong in the operation control and interface (OCX) unit. This unit is located in a big rack under the X-ray counting system, made by ORTEC. The PDP computer used to be under these units in the same rack. Please let us know if you have the circuit diagram of the OCX unit. We would also appreciate any ideas to revive our system. With my best regards:
Laszlo Varga, computer programmer
P.S. I am sending this message to the microscopy and to the EPMA lists at the same time so I would like to apologize to those of you who receive it twice.
==============================Original Headers============================== 5, 21 -- From varguc-at-freemail.hu Wed Jul 20 07:49:42 2011 5, 21 -- Received: from fmx03.freemail.hu (fmx03.freemail.hu [195.228.245.53]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p6KCngqx014698 5, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:49:42 -0500 5, 21 -- Received: (qmail 57168 invoked from network); 20 Jul 2011 14:49:41 +0200 5, 21 -- Received: from 195.228.245.211 (HELO localhost) (193.68.41.17) 5, 21 -- by fmx03.freemail.hu with SMTP; 20 Jul 2011 14:49:41 +0200 5, 21 -- Date: Wed, 20 Jul 2011 14:49:40 +0200 (CEST) 5, 21 -- From: Varga Laszlo {varguc-at-freemail.hu} 5, 21 -- Subject: JCXA 733 operation console schematics needed 5, 21 -- To: Microscopy-at-microscopy.com 5, 21 -- cc: gnagy {gnagy-at-sparc.core.hu} , 5, 21 -- =?ISO-8859-2?Q?Geok=E9mia?= {dobosi-at-geochem.hu} , 5, 21 -- =?ISO-8859-2?Q?Valkai_Zolt=E1n?= {valkai.zoltan-at-kvk.uni-obuda.hu} 5, 21 -- Message-ID: {freemail.20110720144940.895.4-at-xmldata06.freemail.hu} 5, 21 -- X-Originating-IP: [193.68.41.17] 5, 21 -- X-HTTP-User-Agent: Mozilla/4.0 (compatible; MSIE 8.0; Windows NT 6.1; Win64; x64; Trident/4.0; .NET CLR 2.0.50727; SLCC2; .NET CLR 3.5.30729; .NET CLR 3.0.30729; Media Center PC 6.0; .NET4.0C; .NET4.0E) 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Type: TEXT/plain; CHARSET=ISO-8859-2 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from QUOTED-PRINTABLE to 8bit by ns.microscopy.com id p6KCngqx014698 ==============================End of - Headers==============================
Ion pumps have a long, but limited lifetime. Most likely internals of your pump became coated with dielectric deposits, and you will need to replace it. If new pump is beyond the budget, then you can send your original pump to be rebuild.
For rebuilding I wholeheartedly recommend http://www.duniway.com/ No any connections to Duniway here - just a very happy customer.
Good luck :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 7/20/2011 8:21 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both bengu-at-fen.bilkent.edu.tr as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: bengu-at-fen.bilkent.edu.tr Name: Erman Bengu } } Organization: Bilkent University } } Title-Subject: [Filtered] Need help with Ion Pump problem on a EVO40 } } Message: Hi All, } } We are currently experiencing a problem with our Carl-Zeiss EVO 40 SEM. } We have fitted this SEM with a LaB6 filament, and the gun area is pumped } with an ion pump. We occasionally (every 3-4 months) bake the gun area } to reach ~ 2-3 x 10(-7) mbar vacuum levels as a req for the LaB6 } filament operation. } During the last bake, we found that the ion pump stopped working during } the cool down stage while the final vacuum level in the system was good } around 10(-7) mbar levels. } However, since then we were not able to keep the ion-pump working. When } we turn the ion pump on, it slowly dies away. That is, the reading of } the vacuum level in the gun area is thru the ion current (there is not } an extra ion gauge in the system), and the vacuum level starts at } 10(-8)mbars and within 5 mins goes down to 10(-11)mbars. Finally, the } ion pump shuts down. This pattern repeated after several restarts of the } system. } I tried gently tapping the ion pump with a plastic handle of a } screwdriver, which kept vacuum level around 10(-8)'s and the pump } running, but I think that is not a viable option for running any SEM } with decent resolution. :) } } Any ideas, suggestions ? } } Thanx in advance } } Erman Bengu } } Login Host: 139.179.97.107 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jul 20 07:20:21 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KCKKtX016346 } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:20:20 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id D93413E0213 } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:20:20 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id 5JTiImx5MkIs for {microscopy-at-microscopy.com} ; } 12, 25 -- Wed, 20 Jul 2011 07:20:16 -0500 (CDT) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 40D5A3E0208 } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:20:16 -0500 (CDT) } 12, 25 -- Message-ID: {4E26C800.9010904-at-microscopy.com} } 12, 25 -- Date: Wed, 20 Jul 2011 07:20:16 -0500 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: bengu-at-fen.bilkent.edu.tr } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW:Need help with Ion Pump problem on a EVO40 } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
Gentle taps with a screw driver? I can remember the Phillips engineers hitting the magnet of the ion pump on the Bristol EM400 with a 2" by 4" block of wood. This was to knock the whiskers off the inside of the pump which tended to cause it to short out and not operate. If you are going to have to replace the pump anyway, a couple of fairly brutal knocks might be in order, just to see if you can eek out a couple more months. A rebuild is probably in the offing, but you might be able to stretch the life of the current one. Cheers Jfm
On Jul 20, 2011, at 9:52 AM, vray-at-partbeamsystech.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Erman, } } Ion pumps have a long, but limited lifetime. Most likely internals of } your pump became coated with dielectric deposits, and you will need to } replace it. If new pump is beyond the budget, then you can send your } original pump to be rebuild. } } For rebuilding I wholeheartedly recommend http://www.duniway.com/ No any } connections to Duniway here - just a very happy customer. } } Good luck :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 7/20/2011 8:21 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both bengu-at-fen.bilkent.edu.tr as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: bengu-at-fen.bilkent.edu.tr Name: Erman Bengu } } } } Organization: Bilkent University } } } } Title-Subject: [Filtered] Need help with Ion Pump problem on a EVO40 } } } } Message: Hi All, } } } } We are currently experiencing a problem with our Carl-Zeiss EVO 40 SEM. } } We have fitted this SEM with a LaB6 filament, and the gun area is pumped } } with an ion pump. We occasionally (every 3-4 months) bake the gun area } } to reach ~ 2-3 x 10(-7) mbar vacuum levels as a req for the LaB6 } } filament operation. } } During the last bake, we found that the ion pump stopped working during } } the cool down stage while the final vacuum level in the system was good } } around 10(-7) mbar levels. } } However, since then we were not able to keep the ion-pump working. When } } we turn the ion pump on, it slowly dies away. That is, the reading of } } the vacuum level in the gun area is thru the ion current (there is not } } an extra ion gauge in the system), and the vacuum level starts at } } 10(-8)mbars and within 5 mins goes down to 10(-11)mbars. Finally, the } } ion pump shuts down. This pattern repeated after several restarts of the } } system. } } I tried gently tapping the ion pump with a plastic handle of a } } screwdriver, which kept vacuum level around 10(-8)'s and the pump } } running, but I think that is not a viable option for running any SEM } } with decent resolution. :) } } } } Any ideas, suggestions ? } } } } Thanx in advance } } } } Erman Bengu } } } } Login Host: 139.179.97.107 } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jul 20 07:20:21 2011 } } 12, 25 -- Received: from znl.com ([206.69.208.20]) } } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KCKKtX016346 } } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:20:20 -0500 } } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } } 12, 25 -- by znl.com (Postfix) with ESMTP id D93413E0213 } } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:20:20 -0500 (CDT) } } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 12, 25 -- Received: from znl.com ([127.0.0.1]) } } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } } 12, 25 -- with ESMTP id 5JTiImx5MkIs for {microscopy-at-microscopy.com} ; } } 12, 25 -- Wed, 20 Jul 2011 07:20:16 -0500 (CDT) } } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } } 12, 25 -- by znl.com (Postfix) with ESMTPA id 40D5A3E0208 } } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 07:20:16 -0500 (CDT) } } 12, 25 -- Message-ID: {4E26C800.9010904-at-microscopy.com} } } 12, 25 -- Date: Wed, 20 Jul 2011 07:20:16 -0500 } } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } } 12, 25 -- Reply-To: bengu-at-fen.bilkent.edu.tr } } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 12, 25 -- MIME-Version: 1.0 } } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 12, 25 -- Subject: viaWWW:Need help with Ion Pump problem on a EVO40 } } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 12, 25 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 6, 40 -- From vray-at-partbeamsystech.com Wed Jul 20 08:43:41 2011 } 6, 40 -- Received: from nm27-vm3.bullet.mail.ne1.yahoo.com (nm27-vm3.bullet.mail.ne1.yahoo.com [98.138.91.157]) } 6, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p6KDhexJ031474 } 6, 40 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 08:43:40 -0500 } 6, 40 -- Received: from [98.138.90.48] by nm27.bullet.mail.ne1.yahoo.com with NNFMP; 20 Jul 2011 13:43:39 -0000 } 6, 40 -- Received: from [98.138.89.244] by tm1.bullet.mail.ne1.yahoo.com with NNFMP; 20 Jul 2011 13:43:39 -0000 } 6, 40 -- Received: from [127.0.0.1] by omp1058.mail.ne1.yahoo.com with NNFMP; 20 Jul 2011 13:43:39 -0000 } 6, 40 -- X-Yahoo-Newman-Id: 474885.86389.bm-at-omp1058.mail.ne1.yahoo.com } 6, 40 -- Received: (qmail 77645 invoked from network); 20 Jul 2011 13:43:39 -0000 } 6, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1311169419; bh=JDm6OSnAmt5klzqQF60lo9oLJ3X2q8suSpvgsYLezhs=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=Km4ymsGr3H7c8TxQ18/kih/LK9DR+5cjYoTOIOwxBkAh//3pXlY58BdrAmeJgK0i8jS0OzwLxDYOa5llcMJwZZDP0DqeT8vZqCBu53aLFlTUvHHJ9sHi43f4QO0FyYioybkTWnkwRyHnDjcqY9zEumvR+83Yi2X24O+32Yc5GZg= } 6, 40 -- X-Yahoo-Newman-Property: ymail-3 } 6, 40 -- X-YMail-OSG: UZHehyIVM1k9ycF4hjUCTw67y5x2gZ9k6rS9ekDVsJbD5My } 6, 40 -- 9LzApgId10jF46qlNC2GMN9QRqNtaSmVPhHRjEQJrNfCmHTg_KLYgdJMyfN4 } 6, 40 -- phmJSzgw3l3vskOfOABecOh6iBn7uz23E.1vT291Of9HHfmNpB4axQHPifAn } 6, 40 -- HGQXbrkpdBOj9Mx0xgzHNme_3R5u9cB.6oGO0vAqqYaT1FwmW0G80USp84Qs } 6, 40 -- PiOEgdmAu6kBpj9rNwjTbXGXtYez8z4rQJKPhwx8.3JLC2.glPSuMz.pGE8I } 6, 40 -- WQ__2Y.nxwM7Y5Dir8XaiaN23crnPYNe1iGZdkOANUJSQdJhatjBaNO.5bsj } 6, 40 -- pyQ6jJZWurXtROjN.ulVkEtraKc8icrb0j7jGSZnEbnheDUzmeheludsHx96 } 6, 40 -- BJkv_Yo2uF_g.NmTYd3NPpOkzq_Sj1IZprU2SzWO4ErQv58ZCZpfYCQD1UVN } 6, 40 -- uvg9Ww.t0A4KIux4w6WHJlCgKZ5NfqvE6__TGwRth9A53vYLc3Ux5wfuCplw } 6, 40 -- dU.fSQ4DfP.PmTQRBMF3PqT7NPVBstpKiiy56EtM4RqOmsIOcjowUTZJJl65 } 6, 40 -- PZIjWxGLBO_KkhCLVNb_8RW6Ggg_tCfcw9DfM2HbM8e8C9NKJhEDgvlC9mhk } 6, 40 -- AHu0J20Ltn_om7uCqamb_LeZ_Nkv3ALrMCEgk11VG0HN9YOEDh8E4cGa.BOL } 6, 40 -- mJ5kuTJTS2ms17N9alJCjclEg80pxkvAAVesBy9U- } 6, 40 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- } 6, 40 -- Received: from [192.168.2.2] (vray-at-75.68.231.25 with plain) } 6, 40 -- by smtp114.biz.mail.mud.yahoo.com with SMTP; 20 Jul 2011 06:43:38 -0700 PDT } 6, 40 -- Message-ID: {4E26DB8D.4010303-at-partbeamsystech.com} } 6, 40 -- Date: Wed, 20 Jul 2011 09:43:41 -0400 } 6, 40 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} } 6, 40 -- Reply-To: vray-at-partbeamsystech.com } 6, 40 -- Organization: PBS&T } 6, 40 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Lightning/1.0b2 Thunderbird/3.1.11 } 6, 40 -- MIME-Version: 1.0 } 6, 40 -- To: microscopy-at-microscopy.com, bengu-at-fen.bilkent.edu.tr } 6, 40 -- Subject: Re: [Microscopy] viaWWW:Need help with Ion Pump problem on a EVO40 } 6, 40 -- References: {201107201221.p6KCLWYd019627-at-ns.microscopy.com} } 6, 40 -- In-Reply-To: {201107201221.p6KCLWYd019627-at-ns.microscopy.com} } 6, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 6, 40 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } }
___ John Mansfield PhD Cphys MInstP Microanalysis Society - President Elect Associate Director North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 9, 20 -- From jfmjfm-at-umich.edu Wed Jul 20 09:00:48 2011 9, 20 -- Received: from tombraider.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.12.86]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KE0lYB015524 9, 20 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 09:00:47 -0500 9, 20 -- Received: FROM jfm-mbp2.engin.umich.edu (jfm-mbp2.engin.umich.edu [141.213.21.70]) 9, 20 -- By tombraider.mr.itd.umich.edu ID 4E26DF8D.6739B.2109 ; 9, 20 -- Authuser jfmjfm; 9, 20 -- 20 Jul 2011 10:00:45 EDT 9, 20 -- Subject: Re: [Microscopy] Re: viaWWW:Need help with Ion Pump problem on a EVO40 9, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 9, 20 -- Content-Type: text/plain; charset=iso-8859-1 9, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 9, 20 -- In-Reply-To: {201107201352.p6KDqAdG012638-at-ns.microscopy.com} 9, 20 -- Date: Wed, 20 Jul 2011 10:00:45 -0400 9, 20 -- Message-Id: {DAAB8CA4-20E2-4D0E-977D-986D19C98A38-at-umich.edu} 9, 20 -- References: {201107201352.p6KDqAdG012638-at-ns.microscopy.com} 9, 20 -- To: microscopy-at-microscopy.com 9, 20 -- X-Mailer: Apple Mail (2.1084) 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6KE0lYB015524 ==============================End of - Headers==============================
Erman; Use a hammer and pound the crap out of it. If you are doing it right, you should also use hearing protection and eye protection.
A. John Mardinly, ASU
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, July 20, 2011 5:35 AM To: John Mardinly
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both bengu-at-fen.bilkent.edu.tr as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Title-Subject: [Filtered] Need help with Ion Pump problem on a EVO40
Message: Hi All,
We are currently experiencing a problem with our Carl-Zeiss EVO 40 SEM. We have fitted this SEM with a LaB6 filament, and the gun area is pumped
with an ion pump. We occasionally (every 3-4 months) bake the gun area to reach ~ 2-3 x 10(-7) mbar vacuum levels as a req for the LaB6 filament operation. During the last bake, we found that the ion pump stopped working during the cool down stage while the final vacuum level in the system was good around 10(-7) mbar levels. However, since then we were not able to keep the ion-pump working. When we turn the ion pump on, it slowly dies away. That is, the reading of the vacuum level in the gun area is thru the ion current (there is not an extra ion gauge in the system), and the vacuum level starts at 10(-8)mbars and within 5 mins goes down to 10(-11)mbars. Finally, the ion pump shuts down. This pattern repeated after several restarts of the
system. I tried gently tapping the ion pump with a plastic handle of a screwdriver, which kept vacuum level around 10(-8)'s and the pump running, but I think that is not a viable option for running any SEM with decent resolution. :)
Took me 20 minutes to burrow into the Leica site - back-and-forth between Google and Leica - before I got a link that gave a Canadian address following an ultramicrotome 'hit'
If I didn't know better, from your experience and mine today, I might think that Leica doesn't want anyone to contact them on this subject. If the owner is a public university, then get the lawyers to make the call, and make certain they have your service contract in hand.
Hope this helps,
Fred Monson
HTTP://CMIRT.WCUPA.EDU fmonson-at-wcupa.edu
P.S. And I've been a Leitz user and owner all of my life!
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, July 20, 2011 8:32 AM To: Monson, Frederick
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] service ultramicrotome
Message: Hi, I posted a message asking about ultramicrotomes and I also sent e-mails to EM labs in my area. Depending on what model of Reichetr-Jung you have there might or might not be a service available. I got a contact for Leica Microsystems and they agreed to service it. I have Ultracut E and apparantly there is no service available at all. Dorota
If it's any use, I was told that Leica's specimen sectioning/preparation business is now handled by Mager Scientific. Their contact information is below:
www.magersci.com Questions? Email us or call 734.426.3885 Mager Scientific, Inc. P.O. Box 160 Dexter, Michigan 48130
I don't believe they service the ultramicrotomes, but they have contacts for parts, and possibly a third party vendor recommendation.
Good luck, ~Gregg
Gregg Sobocinski Microscope Imaging Specialist University of Michigan Molecular, Cellular, and Developmental Biology Ann Arbor, Michigan USA
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] service ultramicrotome
Message: Hi, I posted a message asking about ultramicrotomes and I also sent e-mails to EM labs in my area. Depending on what model of Reichetr-Jung you have there might or might not be a service available. I got a contact for Leica Microsystems and they agreed to service it. I have Ultracut E and apparantly there is no service available at all. Dorota
We routinely store small ampoules of our glutaraldehyde in the freezer. Someone noticed that 4 out of the 20 or so ampoules did not freeze even after 4 months. We first thought they must be different concentrations, but they are all 8%.
Anyone know why this might occur?
Thank you.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 5, 18 -- From bozzola-at-siu.edu Wed Jul 20 17:38:53 2011 5, 18 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KMcqZo031776 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 17:38:53 -0500 5, 18 -- Received: by iwn8 with SMTP id 8so739562iwn.0 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 15:38:52 -0700 (PDT) 5, 18 -- MIME-Version: 1.0 5, 18 -- Received: by 10.231.111.36 with SMTP id q36mr8218961ibp.142.1311201532299; 5, 18 -- Wed, 20 Jul 2011 15:38:52 -0700 (PDT) 5, 18 -- Received: by 10.42.246.135 with HTTP; Wed, 20 Jul 2011 15:38:52 -0700 (PDT) 5, 18 -- Date: Wed, 20 Jul 2011 17:38:52 -0500 5, 18 -- Message-ID: {CAKeCJ9z7LQUg6QdrVtapO5guO9SPxrxM24UdbsQZK_t_WyYRVQ-at-mail.gmail.com} 5, 18 -- Subject: EM: frozen glutaraldehyde question 5, 18 -- From: John Bozzola {bozzola-at-siu.edu} 5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 18 -- Content-Type: text/plain; charset=UTF-8 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6KMcqZo031776 ==============================End of - Headers==============================
I don't have the answer but will share a similar observation with Mowiol mounting medium. I freeze 2 ml aliquots of a batch and store them all in one rack in my -20 C freezer. Invariably some freeze and others don't. It doesn't appear to be related to where they are stored in the freezer. Initially I blamed this on technicians who failed to adequately mix the ingredients. But then I made a batch and stirred it for a ridiculous amount of time and still observed the variability. Perhaps the answer to John's question will also solve my mystery. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu] Sent: Wednesday, July 20, 2011 5:40 PM To: Phillips, Thomas E.
We routinely store small ampoules of our glutaraldehyde in the freezer. Someone noticed that 4 out of the 20 or so ampoules did not freeze even after 4 months. We first thought they must be different concentrations, but they are all 8%.
Anyone know why this might occur?
Thank you.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 5, 18 -- From bozzola-at-siu.edu Wed Jul 20 17:38:53 2011 5, 18 -- Received: from mail-iw0-f169.google.com (mail-iw0-f169.google.com [209.85.214.169]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6KMcqZo031776 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 17:38:53 -0500 5, 18 -- Received: by iwn8 with SMTP id 8so739562iwn.0 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 15:38:52 -0700 (PDT) 5, 18 -- MIME-Version: 1.0 5, 18 -- Received: by 10.231.111.36 with SMTP id q36mr8218961ibp.142.1311201532299; 5, 18 -- Wed, 20 Jul 2011 15:38:52 -0700 (PDT) 5, 18 -- Received: by 10.42.246.135 with HTTP; Wed, 20 Jul 2011 15:38:52 -0700 (PDT) 5, 18 -- Date: Wed, 20 Jul 2011 17:38:52 -0500 5, 18 -- Message-ID: {CAKeCJ9z7LQUg6QdrVtapO5guO9SPxrxM24UdbsQZK_t_WyYRVQ-at-mail.gmail.com} 5, 18 -- Subject: EM: frozen glutaraldehyde question 5, 18 -- From: John Bozzola {bozzola-at-siu.edu} 5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 18 -- Content-Type: text/plain; charset=UTF-8 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6KMcqZo031776 ==============================End of - Headers==============================
Have you checked to see if you have a non-frosting freezer? Some freezers routinely switch on a thawing cycle to remove ice from the chamber.
You can easily tell of you have one or not - if your freezer is filled with ice, you don't have one. If there is no ice build-up, it is because the machine is thawing it on a time schedule.
Paul.
Paul Webster, Ph.D. House Ear Institute 2100 West Third Street Los Angeles CA 90057 (213) 273 8026
On 7/20/11 3:42 PM, "bozzola-at-siu.edu" {bozzola-at-siu.edu} wrote:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both singinggardenersx2-at-live.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Nonfat Dry Milk Powder for OsO4 Neutralization
Message: Hello Listers, We are in the process of getting set-up for traditional bench top fixation and resin embedding for a special TEM project, and as part of that I have the task of writing an SOP for handling osmium tetroxide. A colleague at the neighboring Lawrence Berkeley National Lab uses nonfat dry milk powder for his OsO4 emergency spill kit. Try as I might, I have not been able to find any literature that describes this as a safe way to neutralize osmium tetroxide -- just the usual corn oil/kitty litter. Have any of you used nonfat dry milk powder for osmium spills, or do you know a source I could quote in our SOP? It seems a very simple and easy way to handle a spill, but I want to make sure this method actually safe before we make it part of our OsO4 handling protocol. Many thanks,
Gigi Kemalyan Research Technician HHMI/Nogales Lab 742 Stanley Hall Berkeley, CA 94720 http://cryoem.berkeley.edu Email (work): gigik-at-berkeley.edu Email (ListServer): singinggardenersx2-at-live.com
Please carefully review the return address when using the "REPLY" feature of your email program for Microscopy Listserver postings.
It is important to note that postings made to the Listserver using the on-line WWW form (i.e. viaWWWW in the Subject line) have a return address of
microscopylistserver-noreply-at-microscopy.com
If you "reply" to this address then it gets delivered to an Email blackhole. No one receives your reply and your valuable comments will be lost.
When there is a posting with a subject line starting with the keyphrase viaWWWW:" you should reply to
Microscopy-at-microscopy.com to have your message copied to the entire list. Alternatively carefully copy and paste the senders address into Email To: line and you will be sending a private reply.
Cheers
Nestor Your Friendly Neighborhood SysOp.
==============================Original Headers============================== 11, 25 -- From zaluzec-at-aaem.amc.anl.gov Wed Jul 20 19:54:34 2011 11, 25 -- Received: from znl.com ([206.69.208.20]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6L0sYf3000520 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 19:54:34 -0500 11, 25 -- Received: from localhost (localhost [127.0.0.1]) 11, 25 -- by znl.com (Postfix) with ESMTP id 81CD93E0962 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 19:54:34 -0500 (CDT) 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 25 -- Received: from znl.com ([127.0.0.1]) 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 25 -- with ESMTP id C01YxPkU5oDy for {microscopy-at-microscopy.com} ; 11, 25 -- Wed, 20 Jul 2011 19:54:33 -0500 (CDT) 11, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 11, 25 -- by znl.com (Postfix) with ESMTPA id DC0B63E0957 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 19:54:33 -0500 (CDT) 11, 25 -- Message-ID: {4E2778C9.1020401-at-aaem.amc.anl.gov} 11, 25 -- Date: Wed, 20 Jul 2011 19:54:33 -0500 11, 25 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 11, 25 -- Organization: ANL 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 25 -- MIME-Version: 1.0 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 25 -- Subject: Administrivia: Replying to postings from microscopylistserver-noreply-at-microscopy.com 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dr. Mardinly's comments about using a hammer to dislodge whiskers inside an ion pump leads me to point out that if the whiskers are not too firmly established it is often possible to get rid of them by turning the high voltage supply on for a few seconds (not too long or you may damage the power supply) three or four times with the pressure in the pump above 1 Pa (10-2 Torr), If this approach works it is gentler, and quieter, than the hammer method. Incidentally, this method (but not the hammer method) and other characteristics of ion pumps, are described on page 295 of my book, 'Vacuum Methods in Electron Microscopy'.
Good luck! -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
this is a good sign! obviously your solution in the state of a supercooled fluid and is very clean (pure) and without any particles (serving as starters for freezing of ice crystals) just tap hardly on the vial immediately after taking from the freezer and usually it should freeze (get solid) within a second.
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ----------------------------------------------------------------------------
We routinely store small ampoules of our glutaraldehyde in the freezer. Someone noticed that 4 out of the 20 or so ampoules did not freeze even after 4 months. We first thought they must be different concentrations, but they are all 8%. Anyone know why this might occur? Thank you.
This is the correct procedure how to remove whiskers, however I do not think that Erman's problem is caused by whiskers in the ion pump. Presence of whiskers causes additional field emission current and as a result it appears like additional (leakage) current. Such ion pump shows higher ion current and therefore it measures higher pressure than expected. Erman's ion pump shows unexpectedly low pressure. Therefore it could be a problem of the ion pump controller (e.g. lower voltage than usual), ion pump is not necessarily bad. I would not use a hammer in this case :-). Erman, please let us know your results later, so that we will know what was your problem. Tomas
On Wed, 20 Jul 2011 20:51:28 -0500 bigelow-at-umich.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dr. Mardinly's comments about using a hammer to dislodge whiskers } inside an ion pump leads me to point out that if the whiskers are } not too firmly established it is often possible to get rid of them by } turning the high voltage supply on for a few seconds (not too long or } you may damage the power supply) three or four times with the } pressure in the pump above 1 Pa (10-2 Torr), If this approach works } it is gentler, and quieter, than the hammer method. Incidentally, } this method (but not the hammer method) and other characteristics of } ion pumps, are described on page 295 of my book, 'Vacuum Methods in } Electron Microscopy'. } } Good luck! } -- } Wilbur C. Bigelow, Professor Emeritus } Materials Sci. & Engr., Univ. of Michigan } Ann Arbor, Michigan 48109-2136 } e-mail: bigelow-at-umich.edu; } Fx:734-763-4788; Ph:734-975-0858 } Address mail to: 2911 Whittier Court } Ann Arbor, MI 48104-6731
as to my knowledge, there are several (US-) (internet-)sources (SOP's, pdf's) on OsO4 handling, storage and disposal available, some of them I have filed in my e-library. e.g.: OsO4 SOP: Div.of Occupatl.Health&Safety,NIH OsO4 SOP: UnivMed&Dent.NJ
Also I have chosen a very simple ("European") way of treating used OsO4 working solutions and disposing them of very effective by first overlaying such with (ca.98-99%) EtOH (ETHANOL) solution (which has been collected from the last two dehydration steps of specimens in 100% EtOH) and in a second step, to decant the more less clear overlaying solution and keep the } Osmium Black { precipitate in a bigger storage flask until it is filled and last but not least can be
EITHER "recycled" (by yourself, colleagues of a Chemistry Department or a company willing to take over the material for recycling)
OR disposed of as "OsO2-metal precipitate in EtOH" according to your national/local laws/regulations.
Such a method IMHO should be favoured (compared to treating OsO4-working solutions with either corn oil, kitty litter and so on....), since it produces more/less safe and stable storage conditions for a more/less non-hazardous precipitation product, which - additionally - easily can be } stored { as well as } recovered { in higher amounts and might be "recycled" by chemical proven methods if such a possibility is desired for any reason.
Such a "method", as I described earlier (MUSS 1992) was not invented by myself, since there were bibliographic sources pointing to such possibilities, but has been tested and proven over a long period of } practical { experience (my own hand on: 1983-1992), was communicated also several times on the MSA listserver, you'll find several postings on this in the MSA List-Server archives [1997, 2002 2007, last thread on this was Nov.2010, as well as some Net-Notes in the MSA-periodical: Microscopy Today..]
You need not to follow such personal practical hints, because they were made "official" by a } European { Electron microscopist like me (opposing at that time the disposal considerations of a big US-Company), but there also have been some US-colleagues using that method independently in parallel and there were given quite strong hints on that "precipitating OsO4 to OsO2= Osmiumblack " method by
e.g. MILLONIG 1976 ( "About Methodology" conc. OsO4 Fixative(s) in: Laboratory Manual of Biological Electron Microscopy, Millonig G (ed.), Mario SAVIOLO EDITORE, Vercelli Italy)
as well as KIERNAN, e.g. 2001 John A. KIERNAN: e-mail: jkiernan-at-julian.uwo.ca Recycling Osmium Tetroxide. MICROSCOPY TODAY, #01-1, 19, (Jan) 2001 keywords: recycling, osmeth, OsO4, OsO2, primary, secondary, post - fixative { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {CITATION} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } Osmium tetroxide is indeed a wonderful stuff. Osmium is a rare element, so disposal of used solutions should consist of recycling, not dumping, even though osmium compounds are not considered environmentally hazardous (Smith et al., 1978, Trace Metal in the Environment, vol. 4, Ann Arbor Science Publishers). The colorless and soluble toxic tetroxide is rapidly reduced by almost any kind of dirt to a black, insoluble dioxide, usually in a colloidal form that's readily dispersed by moving water if it isn't firmly stuck to the solid organic matter that brought about the reduction.
If OsO4 slops are collected in alcohol, the osmium (now in the form of crude, harmless, insoluble osmium dioxide) can be reoxidized, purified, re-reduced to pure OsO2 and stored. OsO2 is easily re-oxidized to give a buffered solution of osmium tetroxide (2% or less). See J. Micr. 113, 77-82 (1978) 1; the procedure does involve certain hazards, so it must be done carefully.
Recovered OsO4 can also be used to make osmeth, which is a beautiful golden crystalline solid that contains osmium tetroxide complexed with methenamine ( = hexamethylene tetramine or hexamine [HANKER et al., 1976, Histochemistry 49, 263-291] 2). It costs next to nothing to make your own osmeth from recycled OsO4, but osmeth is very expensive to buy. Osmeth does not emit osmic (and therefore toxic) fumes. { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {end of citation { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {
but also there are other points of views published in the net: see RE by Jim Darley in 2000) to KIERNAN's view at: http://www.histosearch.com/histonet/Nov00A/RE.quotDisposalquotofOsmiA.html
As a matter of fact, recently (don't exactly know when companies started to offer such reagents) there have been described other ways to fully deactivate as well as recover OsO4-OsO2 by means of chemical scavenger reagent: cf: Osmium recovery is possible with scavengers such as {QuadraPure} (http://www.sigmaaldrich.com/etc/medialib/docs/Aldrich/Brochure/al_quadrapure_guide.Par.0001.File.tmp/al_quadrapure_guide.pdf) Now available from Johnson Matthey on scale as well as Sigma-Aldrich for smaller amounts. http://www.scavengingtechnologies.com/site.asp?id=1297&pageid=1299 (but that means: you have to pay for additional, perhaps costly chemicals)
If you would like to know more about the low cost, effective and reliable "technique" I personally use for now over 25 years in our lab (and got a local award for environment protection!), or would like to get some documents wherein the steps not only have been described but have been enlighting also the "Why's" and "How's", please feel free to reply to me and request/allow sending of those documents (pdf's).
Naturally I would like to hear about what you are going to do, for that: best wishes and good luck,
Wolfgang MUSS (MSA Member in good standing since 1996) Head EM-Lab Univ.-Inst. Pathology SALK-LKH (Gen. Hosp.) and PMU (priv. Paracelsus Medical University)
SALZBURG AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Gesendet: Donnerstag, 21. Juli 2011 01:03 } An: Muß Wolfgang } Betreff: [Microscopy] Nonfat Dry Milk Powder for OsO4 Neutralization } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } This Question/Comment was submitted to the Microscopy Listserver using the } WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both singinggardenersx2-at-live.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } Email: singinggardenersx2-at-live.com } Name: Gigi Kemalyan } Organization: HHMI/Nogales Lab } Title-Subject: Nonfat Dry Milk Powder for OsO4 } Neutralization } Message: } } Hello Listers, } We are in the process of getting set-up for traditional bench top } fixation and resin embedding for a special TEM project, and as part of } that I have the task of writing an SOP for handling osmium tetroxide. } } A colleague at the neighboring Lawrence Berkeley National Lab uses } nonfat dry milk powder for his OsO4 emergency spill kit. } Try as I might, I have not been able to find any literature that } describes this as a safe way to neutralize osmium tetroxide -- just the } usual corn oil/kitty litter. } } Have any of you used nonfat dry milk powder for osmium spills, or do } you know a source I could quote in our SOP? It seems a very simple and } easy way to handle a spill, but I want to make sure this method } actually safe before we make it part of our OsO4 handling protocol. } } Many thanks, } } Gigi Kemalyan } Research Technician } HHMI/Nogales Lab } 742 Stanley Hall } Berkeley, CA 94720 } http://cryoem.berkeley.edu } Email (work): gigik-at-berkeley.edu } Email (ListServer): singinggardenersx2-at-live.com } } } } Login Host: 136.152.21.151 } ----------------------------------------------------------------------- } ---- } } } } ==============================Original } Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Jul 20 } 18:00:34 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p6KN0YvC001106 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 18:00:34 } -0500 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id D8E003E0861 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 18:00:34 } -0500 (CDT) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 11, 25 -- with ESMTP id GwkvWDduIskK for {microscopy-at-microscopy.com} ; } 11, 25 -- Wed, 20 Jul 2011 18:00:29 -0500 (CDT) } 11, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id 25EC73E0856 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jul 2011 18:00:29 } -0500 (CDT) } 11, 25 -- Message-ID: {4E275E0C.4020608-at-microscopy.com} } 11, 25 -- Date: Wed, 20 Jul 2011 18:00:28 -0500 } 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 11, 25 -- Reply-To: singinggardenersx2-at-live.com } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: viaWWW:Nonfat Dry Milk Powder for OsO4 } Neutralization } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 34, 40 -- From W.Muss-at-salk.at Thu Jul 21 03:43:37 2011 34, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 34, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6L8haUD005544 34, 40 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2011 03:43:37 -0500 34, 40 -- Received: from localhost (localhost [127.0.0.1]) 34, 40 -- by hermes.salk.at (Postfix) with ESMTP id 78D7BC3855 34, 40 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2011 10:43:35 +0200 (CEST) 34, 40 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 34, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 34, 40 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 34, 40 -- with ESMTP id bx5jq4dLol-y for {microscopy-at-microscopy.com} ; 34, 40 -- Thu, 21 Jul 2011 10:43:35 +0200 (CEST) 34, 40 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 34, 40 -- by hermes.salk.at (Postfix) with ESMTP id 31E36C3872 34, 40 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2011 10:43:34 +0200 (CEST) 34, 40 -- Received: from n1rz122.lksdom21.lks.local ([192.168.101.122]) 34, 40 -- by N2EX199.lks.local with ESMTP; 21 Jul 2011 10:44:21 +0200 34, 40 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 34, 40 -- Thu, 21 Jul 2011 10:43:34 +0200 34, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 34, 40 -- Content-class: urn:content-classes:message 34, 40 -- MIME-Version: 1.0 34, 40 -- Content-Type: text/plain; 34, 40 -- charset="iso-8859-1" 34, 40 -- Subject: [Microscopy] Re: Nonfat Dry Milk Powder for OsO4 Neutralization [SOP for handling OsO4], sorry for longness 34, 40 -- Date: Thu, 21 Jul 2011 10:43:33 +0200 34, 40 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F32FAE-at-N1RZ116.lksdom21.lks.local} 34, 40 -- In-Reply-To: {201107202302.p6KN2p3B010175-at-ns.microscopy.com} 34, 40 -- X-MS-Has-Attach: 34, 40 -- X-MS-TNEF-Correlator: 34, 40 -- Thread-Topic: [Microscopy] Re: Nonfat Dry Milk Powder for OsO4 Neutralization [SOP for handling OsO4], sorry for longness 34, 40 -- Thread-Index: AcxHMS7x1wD0JgVcTN+iTxfRE8FwggAReTKA 34, 40 -- References: {201107202302.p6KN2p3B010175-at-ns.microscopy.com} 34, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 34, 40 -- To: {singinggardenersx2-at-live.com} 34, 40 -- Cc: {microscopy-at-microscopy.com} 34, 40 -- X-OriginalArrivalTime: 21 Jul 2011 08:43:34.0297 (UTC) FILETIME=[469DD090:01CC4782] 34, 40 -- X-Scanned-By: SALK-Content-Filter 34, 40 -- Content-Transfer-Encoding: 8bit 34, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6L8haUD005544 ==============================End of - Headers==============================
Drats! Here, I thought I had finally discovered proof for dark energy, and was ready to publish a paper on it! I thought the majic particles were keeping these vials thawed. :)
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: pwebster-at-hei.org [pwebster-at-hei.org] Sent: Wednesday, July 20, 2011 7:06 PM To: Haller, Edward
Hi John,
Have you checked to see if you have a non-frosting freezer? Some freezers routinely switch on a thawing cycle to remove ice from the chamber.
You can easily tell of you have one or not - if your freezer is filled with ice, you don't have one. If there is no ice build-up, it is because the machine is thawing it on a time schedule.
Paul.
Paul Webster, Ph.D. House Ear Institute 2100 West Third Street Los Angeles CA 90057 (213) 273 8026
On 7/20/11 3:42 PM, "bozzola-at-siu.edu" {bozzola-at-siu.edu} wrote:
Nah, it's really string theory = the glutaraldehyde has formed long chains.
It hasn't? Then it must be string theory - the predicted value is wrong.
Phil
} Drats! Here, I thought I had finally discovered proof for dark } energy, and was ready to publish a paper on it! I thought the majic } particles were keeping these vials thawed. :) } } Ed } } Edward Haller, Lab Manager } University of South Florida } Integrative Biology Department } Electron Microscopy Core } SCA 110 } 4202 East Fowler Avenue } Tampa, FL 33620 } 813-974-2676 } ehaller-at-usf.edu } Office: LSA 119 } ________________________________________ } X-from: pwebster-at-hei.org [pwebster-at-hei.org] } Sent: Wednesday, July 20, 2011 7:06 PM } To: Haller, Edward } Subject: [Microscopy] Re: EM: frozen glutaraldehyde question } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
I need to etch some Epon from my blocks, and I intended using Sodium Ethoxide for that. Unfortunately, I can't find the detailed protocol that I used (only once) several years ago. Can anyone provide a detailed protocol for the preparation, please? Thanks,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
One more question for today: Has anyone examined EPON blocks in a SEM? I tried to image my muscle samples embedded in EPON with a SEM. (the same block was previously used for ultra-thin sections, so its surface was pretty flat). The only thing I could see were brighter than background spots representing the muscle fibres. I'll try to remove some resin from the surface with sodium ethoxide, so that only the sample "sticks out" a little bit over the resin, unembedded. Has anyone else done something similar (seeing EPON blocks in SEM)? Could you please share your experience?
Thanks again,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
Drats, indeed! Now I have to recall my online paper:
A New Phenomenon: Dark Matter Causes Freezing Point Depression of Aqueous Solutions of Glutaraldehyde
I hoped it would be a classic, along with the articles on polywater and spiritual ectoplasms.
John Bozzola
} } Drats! Here, I thought I had finally discovered proof for dark energy, and was ready to publish a paper on it! I thought the majic particles were keeping these vials thawed. Â :) } } Ed } } Edward Haller, Lab Manager } University of South Florida
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 8, 20 -- From bozzola-at-siu.edu Thu Jul 21 09:14:48 2011 8, 20 -- Received: from mail-gy0-f169.google.com (mail-gy0-f169.google.com [209.85.160.169]) 8, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6LEElEB029335 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Jul 2011 09:14:48 -0500 8, 20 -- Received: by gyg13 with SMTP id 13so742205gyg.0 8, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Jul 2011 07:14:47 -0700 (PDT) 8, 20 -- MIME-Version: 1.0 8, 20 -- Received: by 10.150.2.16 with SMTP id 16mr697876ybb.303.1311257687100; Thu, 21 8, 20 -- Jul 2011 07:14:47 -0700 (PDT) 8, 20 -- Received: by 10.42.246.135 with HTTP; Thu, 21 Jul 2011 07:14:47 -0700 (PDT) 8, 20 -- In-Reply-To: {201107211219.p6LCJRVP002256-at-ns.microscopy.com} 8, 20 -- References: {201107211219.p6LCJRVP002256-at-ns.microscopy.com} 8, 20 -- Date: Thu, 21 Jul 2011 09:14:47 -0500 8, 20 -- Message-ID: {CAKeCJ9wk+K=QgzYxsieW6kQKZx=zsS57-MgEfB=Yx32Z2T3ybA-at-mail.gmail.com} 8, 20 -- Subject: Re: [Microscopy] EM: frozen glutaraldehyde question 8, 20 -- From: John Bozzola {bozzola-at-siu.edu} 8, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 8, 20 -- Content-Type: text/plain; charset=UTF-8 8, 20 -- Content-Transfer-Encoding: 8bit 8, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6LEElEB029335 ==============================End of - Headers==============================
I usually just dump a bunch of sodium hydroxide pellets into the bottle of a glass Schott-style bottle and then add about 50-100 mls of ethanol to it. You want a saturated solution so if all the pellets dissolve, add more. You could use a stir bar to speed equilibration but I usually do it a couple of days beforehand and give the bottle a swirl once or twice a day and that is enough. It is obviously a caustic solution so be careful. Wear gloves and I usually require my students to wear safety glasses when using it. I simply carefully decant off the solution when I want to use it. Many anecdotal comments I have read on line over the years suggest it gets stronger the longer you let the solution sit. When I want to etch the sections, I use this procedure:
1) Pour about 40-50 mls of sodium ethoxide solution into a glass Coplin jar. This solution is very caustic - use gloves and eye protection!
2) Etch number and circle sections on the slides using a diamond pen. Rinse the slides with dH2O.
3) Carefully insert slides into the caustic sodium ethoxide solution using a forceps. Leave in etching solution for 1 hour.
4) Fill 3 Coplin jars with 95% ethanol, 70% ethanol, and dH2O.
5) Using a forceps, transfer the slides from the etching solution into the 95% ethanol for 10 min.
6) Using a forceps, transfer the slides from the 95% solution into the 70% ethanol for 10 min.
7) Using a forceps, transfer the slides to the distilled water Coplin jar. Transfer the Coplin gar to the sink and slowly run distilled water into the jar for 10 min.
8) Let the slides dry.
Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: jmircheski-at-us.es [mailto:jmircheski-at-us.es] Sent: Thursday, July 21, 2011 9:08 AM To: Phillips, Thomas E.
Dear Listers,
I need to etch some Epon from my blocks, and I intended using Sodium Ethoxide for that. Unfortunately, I can't find the detailed protocol that I used (only once) several years ago. Can anyone provide a detailed protocol for the preparation, please? Thanks,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
If you don't mind shipping your equipment try http://southeastpathology.com/service/ for microtome service. best, Beth
} Email: wadowska-at-upei.ca Name: Dorota Wadowska } } Organization: Atlantic Veterinary College at UPEI } } Title-Subject: [Filtered] service ultramicrotome } } Message: Hi, } I posted a message asking about ultramicrotomes and I also sent e- } mails } to EM labs in my area. Depending on what model of Reichetr-Jung you } have } there might or might not be a service available. I got a contact for } Leica Microsystems and they agreed to service it. I have Ultracut E } and } apparantly there is no service available at all. Dorota
==============================Original Headers============================== 4, 19 -- From beth-at-plantbio.uga.edu Thu Jul 21 09:37:45 2011 4, 19 -- Received: from dogwood.plantbio.uga.edu (dogwood.plantbio.uga.edu [128.192.26.2]) 4, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6LEbjip015610 4, 19 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jul 2011 09:37:45 -0500 4, 19 -- Received: from [128.192.26.69] ([128.192.26.69]) 4, 19 -- (authenticated user beth-at-plantbio.uga.edu) 4, 19 -- by dogwood.plantbio.uga.edu 4, 19 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 4, 19 -- for microscopy-at-microscopy.com; 4, 19 -- Thu, 21 Jul 2011 10:37:44 -0400 4, 19 -- Message-Id: {D7D50A9C-270B-4CA6-8B5F-3EA935CC3EBC-at-plantbio.uga.edu} 4, 19 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 4, 19 -- To: microscopy microscopy {microscopy-at-microscopy.com} 4, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 4, 19 -- Content-Transfer-Encoding: 7bit 4, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 4, 19 -- Subject: service ultramicrotome 4, 19 -- Date: Thu, 21 Jul 2011 10:37:45 -0400 4, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society will hold its fifth annual Optical Techniques Workshop on Wednesday, August 31, 2011, at the Hooke College of Applied Sciences in Westmont, IL. John G. Delly will present an overview of contrast techniques for light microscopy. Program details and registration information can be found on our website under Meetings:
www.midwestmicroscopy.org
We look forward to seeing you there!
Regards,
Elaine Schumacher M3S Program Coordinator
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
I haven't seen any replies to your question on the listserver. Yes, I have done SEM on EPON thick sections without etching them, which work better than using the block. Use a razor blade and hand-trim a "thick" section (actually as thin as you can trim manually) from an EPON block after you have cut some thin sections from the block face to smooth the face off, and mount this section with the smooth side facing up on some double-sided carbon tape. Use a piece of plastic wrap (Saran Wrap, sandwich wrap, plastic bag) to press the section onto the tape. If you have access to a carbon coater, give the sample a light carbon coating to reduce charging. Put a couple of marks on the tape so you can find the section easily in the SEM, either by pin pricks or dots of silver paint. Now observe your section using your backscattered electron detector. I've never tried this with en-bloc uranyl acetate stained tissue, only osmicated tissue, but have been able to obtain photos of the tissue in this manner, and if the tissue had foreign particles in it, have located them rapidly by this technique for x-ray analysis. You may try staining your tissue with uranyl acetate and lead citrate and see if this is a possibility. I have not experimented with this.
Ed Haller
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: jmircheski-at-us.es [jmircheski-at-us.es] Sent: Thursday, July 21, 2011 10:21 AM To: Haller, Edward
One more question for today: Has anyone examined EPON blocks in a SEM? I tried to image my muscle samples embedded in EPON with a SEM. (the same block was previously used for ultra-thin sections, so its surface was pretty flat). The only thing I could see were brighter than background spots representing the muscle fibres. I'll try to remove some resin from the surface with sodium ethoxide, so that only the sample "sticks out" a little bit over the resin, unembedded. Has anyone else done something similar (seeing EPON blocks in SEM)? Could you please share your experience?
Thanks again,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball/NYU
Title-Subject: [Filtered] Olympus CKX41 question
Message: The Olympus CKX41 fluorescent tissue culture microscope is sold with filter blocks for "blue" and "green" fluorescence. Has anyone retrofitted it for additional filters, such as mCherry?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both saidel-at-camden.rutgers.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: saidel-at-camden.rutgers.edu Name: Bill Saidel
Organization: Rutgers Univ., Dept of Biology, Camden campus
Title-Subject: [Filtered] converting TEM to digital image capture
Message: Has anyone successfully used the side port of a Zeiss 902 (or any model TEM) with a digital camera body (Nikon) to capture images? If so, please rsvp me with details (saidel-at-camden.rutgers.edu) or information about companies that have the accessory equipment?
The latest Microscopy Today just bounced on my doorstep and I have to say I am 100% with Charles Lyman's editorial comment! And, whilst we are at it, what about destroying the other errors in understanding that have made their home in our subject?
Most errors seem to crop up in SEM terminology. Take objective lens as a name for the third condenser, it does not follow microscopy parlance in that the does not produce the image, it simply acts as a third condenser. In truth it is the instrument's electronics that produces the image. Then there is depth of field when what people are talking about is depth of focus. How does the position of an imaging surface explain the way we visualise depth in an image? Please, please let us sort out depth of field and depth of focus too!
Microscopy has an amazing history, so abusing names that were part of that history does seem so wrong!
I also agree. I bought a microscope and there is nothing micro about it. I can barely lift the thing! :) Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Friday, July 22, 2011 3:14 PM To: Phillips, Thomas E.
Hi
The latest Microscopy Today just bounced on my doorstep and I have to say I am 100% with Charles Lyman's editorial comment! And, whilst we are at it, what about destroying the other errors in understanding that have made their home in our subject?
Most errors seem to crop up in SEM terminology. Take objective lens as a name for the third condenser, it does not follow microscopy parlance in that the does not produce the image, it simply acts as a third condenser. In truth it is the instrument's electronics that produces the image. Then there is depth of field when what people are talking about is depth of focus. How does the position of an imaging surface explain the way we visualise depth in an image? Please, please let us sort out depth of field and depth of focus too!
Microscopy has an amazing history, so abusing names that were part of that history does seem so wrong!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both charlesping-at-hotmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Circuit diagrams of PHI 660
Message: Dear all
Has anyone got circuit diagrams of PHI 660 Scanning Auger System? Our PHI 660 has difficulties in high magnification. The deflection waveform shows high noise. Just wonder if anyone has the circuit diagrams for PHI 660 so that we can diagnose the issue. Many thanks
I agree that the terms "depth of field" and "depth of focus" are too frequently interchanged, but I feel that you, too, have fallen into the same trap. These two terms are well-documented and describe two quite different features in an imaging system. You got it correct in your 1986 book: Working with the Scanning Electron Microscope (page 22).
Depth of field relates to the object space and depth of focus relates to the image space. Some microscopists use "depth of focus" when they really mean "depth of field"; the confusion seems to come from the fact that as the focus control is adjusted, different parts of the specimen (in the z-direction) go in and out of focus. So, when photographers (who first coined the terms) and light and electron microscopists use the term "depth of field" they are correct if they are talking about how much of the subject or specimen is in acceptable focus. Depth of focus is used to describe the how much a camera can be moved from the film /detector plane before the image becomes unacceptably out of focus. Depth of focus is usually a fixed distance that cannot be changed because it is unusual for the microscopist to be able to move the film or detector plane away from the imaging/projection lens. For digital imaging in an SEM, depth of focus is a redundant term because there is no camera, so a microscopist shouldn't even need to think about this term nowadays.
Microscopy does have an amazing history and the two terms are well-established and easily understood, so any attempt to change their meaning will result in even more confusion. I hope that the depth of field and depth of focus is now sorted!
Gary Nichols Pfizer UK ------------------------- Hi
The latest Microscopy Today just bounced on my doorstep and I have to say I am 100% with Charles Lyman's editorial comment! And, whilst we are at it, what about destroying the other errors in understanding that have made their home in our subject?
Most errors seem to crop up in SEM terminology. Take objective lens as a name for the third condenser, it does not follow microscopy parlance in that the does not produce the image, it simply acts as a third condenser. In truth it is the instrument's electronics that produces the image. Then there is depth of field when what people are talking about is depth of focus. How does the position of an imaging surface explain the way we visualise depth in an image? Please, please let us sort out depth of field and depth of focus too!
Microscopy has an amazing history, so abusing names that were part of that history does seem so wrong!
Steve Chapman Protrain
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: 22 July 2011 21:16 To: Nichols, Gary
Hi
The latest Microscopy Today just bounced on my doorstep and I have to say I am 100% with Charles Lyman's editorial comment! And, whilst we are at it, what about destroying the other errors in understanding that have made their home in our subject?
Most errors seem to crop up in SEM terminology. Take objective lens as a name for the third condenser, it does not follow microscopy parlance in that the does not produce the image, it simply acts as a third condenser. In truth it is the instrument's electronics that produces the image. Then there is depth of field when what people are talking about is depth of focus. How does the position of an imaging surface explain the way we visualise depth in an image? Please, please let us sort out depth of field and depth of focus too!
Microscopy has an amazing history, so abusing names that were part of that history does seem so wrong!
Thank you for pointing out a misunderstanding I may have portrayed, my term "imaging surface" was me referring to the "film plane".
For those who I may have confused I think the TEM portrays depth of field and depth of focus very clearly. It is easy to see how small a depth of field the operator has (just microns) and it is clear the depth of focus is almost limitless with an image in focus on a digital camera both above and below the screen!
Watching TV and writing an email is clearly not the best way of explaining a situation; sorry listers.
Steve
-----Original Message----- X-from: gary.nichols-at-pfizer.com [mailto:gary.nichols-at-pfizer.com] Sent: 25 July 2011 11:37 To: protrain-at-emcourses.com
Steve,
I agree that the terms "depth of field" and "depth of focus" are too frequently interchanged, but I feel that you, too, have fallen into the same trap. These two terms are well-documented and describe two quite different features in an imaging system. You got it correct in your 1986 book: Working with the Scanning Electron Microscope (page 22).
Depth of field relates to the object space and depth of focus relates to the image space. Some microscopists use "depth of focus" when they really mean "depth of field"; the confusion seems to come from the fact that as the focus control is adjusted, different parts of the specimen (in the z-direction) go in and out of focus. So, when photographers (who first coined the terms) and light and electron microscopists use the term "depth of field" they are correct if they are talking about how much of the subject or specimen is in acceptable focus. Depth of focus is used to describe the how much a camera can be moved from the film /detector plane before the image becomes unacceptably out of focus. Depth of focus is usually a fixed distance that cannot be changed because it is unusual for the microscopist to be able to move the film or detector plane away from the imaging/projection lens. For digital imaging in an SEM, depth of focus is a redundant term because there is no camera, so a microscopist shouldn't even need to think about this term nowadays.
Microscopy does have an amazing history and the two terms are well-established and easily understood, so any attempt to change their meaning will result in even more confusion. I hope that the depth of field and depth of focus is now sorted!
Gary Nichols Pfizer UK ------------------------- Hi
The latest Microscopy Today just bounced on my doorstep and I have to say I am 100% with Charles Lyman's editorial comment! And, whilst we are at it, what about destroying the other errors in understanding that have made their home in our subject?
Most errors seem to crop up in SEM terminology. Take objective lens as a name for the third condenser, it does not follow microscopy parlance in that the does not produce the image, it simply acts as a third condenser. In truth it is the instrument's electronics that produces the image. Then there is depth of field when what people are talking about is depth of focus. How does the position of an imaging surface explain the way we visualise depth in an image? Please, please let us sort out depth of field and depth of focus too!
Microscopy has an amazing history, so abusing names that were part of that history does seem so wrong!
Steve Chapman Protrain
-----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: 22 July 2011 21:16 To: Nichols, Gary
Hi
The latest Microscopy Today just bounced on my doorstep and I have to say I am 100% with Charles Lyman's editorial comment! And, whilst we are at it, what about destroying the other errors in understanding that have made their home in our subject?
Most errors seem to crop up in SEM terminology. Take objective lens as a name for the third condenser, it does not follow microscopy parlance in that the does not produce the image, it simply acts as a third condenser. In truth it is the instrument's electronics that produces the image. Then there is depth of field when what people are talking about is depth of focus. How does the position of an imaging surface explain the way we visualise depth in an image? Please, please let us sort out depth of field and depth of focus too!
Microscopy has an amazing history, so abusing names that were part of that history does seem so wrong!
Hi Steve, 'Microns' of depth of field was a long time before aberration correctors became popular. I think this discussion of depth of field will become more and more important as correctors allow larger convergence angles and subsequently severely reduce the depth of field. Furthermore the effect of sample thickness, convergence angle and also channeling will become more and more important especially due to the popular pass-time of analyzing at the atomic level directly down a zone-axis. In terms of systems with probe correctors, the depth of field goes to a number in the nanometer range. Well perhaps a few tens of nanometers...
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dzhou-at-tcd.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dzhou-at-tcd.ie Name: Dan Zhou
Organization: TCD & CRANN, Ireland
Title-Subject: [Filtered] TomoJ - 3D tomography
Message: Hi, all.
I'm using TomoJ in ImageJ to do 3D tomography. But when I import the tilt series into ImageJ, the later images will turn very black although originally it is not.
The most likely problem is that you have imported an image with a dc offset or gain setting which does not match the display and the total range of intensities is outside the display capabilities.
This can happen when for example you import 8 bit images into an 16 bit display mode.
In ImageJ go to the pull down menus
Image-} Adjust-} Brightness/Contrast
Try the Auto setting and see if that fixes your data display range.
Cheers
Nestor Your Friendly Neighborhood SysOp
=========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 9700 S. Cass Ave. Argonne, Illinois 60439 USA Tel: 530-NES-TORZ (530-637-8679) Fax: 630-252-4798
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America Senior Fellow of the Computational Institute - University of Chicago E.P. Wigner Fellow - Oak Ridge National Laboratory President: Microscopy Society of America ===========================================
==============================Original Headers============================== 18, 25 -- From Zaluzec-at-aaem.amc.anl.gov Mon Jul 25 08:38:52 2011 18, 25 -- Received: from aaem.amc.anl.gov (aaem.amc.anl.gov [146.139.72.3]) 18, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6PDcpg8029813 18, 25 -- for {microscopy-at-microscopy.com} ; Mon, 25 Jul 2011 08:38:51 -0500 18, 25 -- Received: from localhost (localhost [127.0.0.1]) 18, 25 -- by aaem.amc.anl.gov (Postfix) with ESMTP id B628A2FBA979; 18, 25 -- Mon, 25 Jul 2011 08:38:50 -0500 (CDT) 18, 25 -- X-Virus-Scanned: amavisd-new at aaem.amc.anl.gov 18, 25 -- Received: from aaem.amc.anl.gov ([127.0.0.1]) 18, 25 -- by localhost (aem005.amc.anl.gov [127.0.0.1]) (amavisd-new, port 10024) 18, 25 -- with ESMTP id 5t7jpeIk0pDk; Mon, 25 Jul 2011 08:38:49 -0500 (CDT) 18, 25 -- Received: from [206.69.208.22] (msdvpn005.msd.anl.gov [130.202.251.5]) 18, 25 -- by aaem.amc.anl.gov (Postfix) with ESMTPA id 393232FBA96E; 18, 25 -- Mon, 25 Jul 2011 08:38:49 -0500 (CDT) 18, 25 -- From: "Nestor J. Zaluzec" {Zaluzec-at-aaem.amc.anl.gov} 18, 25 -- Content-Type: text/plain; charset=us-ascii 18, 25 -- Subject: Re: [Microscopy] viaWWW:TomoJ 3D tomography 18, 25 -- Date: Mon, 25 Jul 2011 08:38:48 -0500 18, 25 -- Message-Id: {A0531AB0-556C-4664-AAFD-19CF701B13D7-at-aaem.amc.anl.gov} 18, 25 -- Cc: "Nestor J. Zaluzec" {Zaluzec-at-aaem.amc.anl.gov} 18, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} , dzhou-at-tcd.ie 18, 25 -- Mime-Version: 1.0 (Apple Message framework v1084) 18, 25 -- X-Mailer: Apple Mail (2.1084) 18, 25 -- Content-Transfer-Encoding: 8bit 18, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6PDcpg8029813 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both edsworth-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: edsworth-at-aol.com Name: Ed Holdsworth
Organization: SEMTEC Laboratories, Inc. also MAS
Title-Subject: [Filtered] Need part for JEOL 6100
Message: I need a magnification power amplifier (steel box with lots of stuff in it) for a JEOL 6100. If anybody is piecing out either a JEOL 6100 or a JEOL 820 (not 840) and would like to sell the mag pwr amp, please contact me off line at 602-276-6138. Thanks! Login Host: 65.121.29.2 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Jul 26 07:39:32 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6QCdWu5030864 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2011 07:39:32 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 0DFAF3E503E 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2011 07:39:32 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id nCHms4e3YDf6 for {microscopy-at-microscopy.com} ; 7, 25 -- Tue, 26 Jul 2011 07:39:29 -0500 (CDT) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id A7A933E5033 7, 25 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2011 07:39:29 -0500 (CDT) 7, 25 -- Message-ID: {4E2EB581.6080801-at-microscopy.com} 7, 25 -- Date: Tue, 26 Jul 2011 07:39:29 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: edsworth-at-aol.com 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:Need part for JEOL 6100 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both drg.mitchell-at-sydney.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: drg.mitchell-at-sydney.edu.au Name: David Mitchell
Organization: University of Sydney
Title-Subject: [Filtered] TEM: RDF Software
Message: Dear Listers Radial Distribution Function (RDF) analysis is a powerful tool for understanding structure in amorphous materials. The methods for RDF analysis of electron diffraction data are well established, although to the uninitiated they may seem quite daunting. To help demystify the technique and make its implementation quick and simple, myself and Tim Petersen have developed a suite of tools for RDF analysis in DigitalMicrograph (Gatan inc.). The software is called RDFTools and can be downloaded freely from the following site:
http://www.dmscripting.com/rdftools.html
There is a detailed instruction manual and some sample data to work through. I hope you find it of interest. Regards, Dave Mitchell TEM Manager ACMM, University of Sydney.
New YorkMicroscopical Society Bernard Friedman Memorial Workshops Use of the Microscope& Polarized Light Microscopy  September 17, 24, October 1, 8, 15, 22, 29, 2011  A basic course on light microscopy which will cover the following topics: Theory of microscopy, Kohler Illumination Diffraction Theory, Contrast Methods          Polarized light, Phase Contrast, Interference     Hoffman contrast, Rheinberg, Dark-field & oblique Illumination  An advanced course on polarized light microscopy which will cover the following topics: The nature of polarized light The origin and interpretation of interference colors Birefringence and crystal orientation, The Indicatrix Compensation and variable compensators Interference figures and their interpretation  The workshop will consist of seven consecutive Saturdays of lectures and hands on labs to cover the theoretical and practical aspects of microscopy. The course instructors are Jan Hinsch formerly of Leica Microsystems, Inc., Dennis O’Leary of Micro-Optical Methods, Mary McCann of McCann Imaging, John Reffner of John Jay College and N.Y.M.S. Instructor Don O'Leary.  WHEN:        September 17, 24, October 1 ,8, 15, 22, 29, 2011. 10AM to 4 PM  WHERE:      One Prospect Village Plaza, Clifton, NJ 07013, accessible by public transportation. Information on car pools and transportation will be provided.)  COST:           $695 for NYMS members, $725 for non-members (includes membership) Lunch and course materials are included. Checks made out to NYMS.
HOW:            Register using form below. Limited to the first 12 registrants.     Send form to Don O'Leary, 10 Sampson Street, Unit 113, Saddle Brook, NJ 07663  FURTHER INFORMATION: Call D. O'Leary (201) 368 -8849 E-mail: dkoleary-at-verizon.net Fax (425) 988-1415  PLEASE MAIL THIS APPLICATION WITH YOUR PAYMENT ------------------------------------------------------------------------- Registration Form Use of the Microscope & Polarized Light Microscopy N.Y.M.S. Member_________________ ($695) Non-Member__________($725) Sept. 17 to Oct. 29 Registration for Use of the Microscope only (4 Sessions) N.Y.M.S. Member_________________ ($395) Non-Member__________($425) Sept. 17 to Oct. 8 Registration for Polarized Light Microscopy Only (4 Sessions) N.Y.M.S. Member_________________ ($395) Non-Member__________($425) Oct. 8 to 29 Name______________________________________________________________________ Address____________________________________________________________________ City___________________________State_________________zip____________ Phone (W)_______________________(H)___________________________  e-mail address___________________.  Please send your application and payment directly to:  NYMS Fall 2011 Courses c/o Mel Pollinger, Treasurer 18-04 Hillery Street Fair Lawn, NJ 07410-5207                  Â
==============================Original Headers============================== 3, 31 -- From dkoleary-at-verizon.net Tue Jul 26 15:54:45 2011 3, 31 -- Received: from nm14.access.bullet.mail.mud.yahoo.com (nm14.access.bullet.mail.mud.yahoo.com [66.94.237.215]) 3, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p6QKsjfY005548 3, 31 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jul 2011 15:54:45 -0500 3, 31 -- Received: from [66.94.237.197] by nm14.access.bullet.mail.mud.yahoo.com with NNFMP; 26 Jul 2011 20:54:45 -0000 3, 31 -- Received: from [66.94.237.98] by tm8.access.bullet.mail.mud.yahoo.com with NNFMP; 26 Jul 2011 20:54:45 -0000 3, 31 -- Received: from [127.0.0.1] by omp1003.access.mail.mud.yahoo.com with NNFMP; 26 Jul 2011 20:54:45 -0000 3, 31 -- X-Yahoo-Newman-Property: ymail-3 3, 31 -- X-Yahoo-Newman-Id: 178562.64273.bm-at-omp1003.access.mail.mud.yahoo.com 3, 31 -- Received: (qmail 5497 invoked by uid 60001); 26 Jul 2011 20:54:45 -0000 3, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1311713685; bh=NCOmrNY2bmrTIFXG9kkCIy/As0GUf4p4tLRnz3Im90c=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=dwB7tbLA0GOPzv7NJ+CPiqn1ZytK7kQK6KoRgD4MW7qsXRt3PG8Fy37TRtnUwL+ZXqLUayhOeS55w+k8UqmleOBilF+TvvtqfhkY+yt4P69GnZXL129oKUS0JvgSxxCVB+Bo6pecOOq+ur4gsQQ6v8abOv8sp+DOEFTwMkfSLUg= 3, 31 -- X-YMail-OSG: O_wjHdMVM1nXauX1bvPs3MPOyUoQvIJI7aBBCXJnSwwoGwM 3, 31 -- j_2kIBRE2uQT_VSbQd76xrLfEghgKzLNL53HY2F0YQOKQTsrjvhS.IpgZ53M 3, 31 -- 0OLUyv9Zp6ES3ToU4VRKqBZjxQCPckVkO9IfnfjFi.547FU_9.XkD_8PsL7M 3, 31 -- QgnmybDzDZ02gLOGJkd2a4.7VSIIDANgL4ma8aVuoTh5Eu9uTEUsi42Ot.N8 3, 31 -- LOVZsmvjS6IVYbZ2YtN2zhyuOEbU72aiY63XJblbXEfWZddccpEj8C5apL24 3, 31 -- 0r3ak0Nd3Zd_PJQCRXs._cJX1.AHSRr_HoHrrszcmgfsM3NogwDWUv06CPQF 3, 31 -- rN15ts0GMoJkLG.kvIZf2KKeLCjFxddeEeTuDOBbH0TwUWVu5sIN8qV_TWrg 3, 31 -- - 3, 31 -- Received: from [68.239.207.181] by web84004.mail.mud.yahoo.com via HTTP; Tue, 26 Jul 2011 13:54:44 PDT 3, 31 -- X-Mailer: YahooMailWebService/0.8.112.310352 3, 31 -- Message-ID: {1311713684.95050.YahooMailNeo-at-web84004.mail.mud.yahoo.com} 3, 31 -- Date: Tue, 26 Jul 2011 13:54:44 -0700 (PDT) 3, 31 -- From: DONALD OLEARY {dkoleary-at-verizon.net} 3, 31 -- Reply-To: DONALD OLEARY {dkoleary-at-verizon.net} 3, 31 -- Subject: LM NYMS 7 Saturday course on Use of & polarized light Microscopy 3, 31 -- To: Microscopy List server {Microscopy-at-microscopy.com} 3, 31 -- MIME-Version: 1.0 3, 31 -- Content-Type: text/plain; charset=utf-8 3, 31 -- Content-Transfer-Encoding: 8bit 3, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6QKsjfY005548 ==============================End of - Headers==============================
I would like to make you aware of the Sunday (Aug. 7, 8:30 AM-5 PM) short course on 3D electron microscopy held at the Microcopy and Microanalysis meeting Aug 7-11 in Nashville TN. This course should not only be interesting to biologists, but also for people in the material sciences field interested in 3D electron microscopy. Registration for this course is still open (http://www.microscopy.org/MandM/2011/register.cfm). Description below.
X14: 3D Electron Microscopy of Macromolecular Assemblies Teresa Ruiz, Michael Radermacher
This short course will provide a comprehensive description of the methods used for 3D structure determination of macromolecular complexes from electron micrographs. First specimen preparation techniques for single particles (deep stain, vitreous ice), will be presented and the selection of imaging conditions including low-dose imaging. This will be followed by a detailed explanation of image processing techniques with special emphasis on the random conical reconstruction technique. In the last part structure interpretation and docking of X-ray structures to 3D EM densities will be demonstrated.
The techniques described have application in both biological and materials science.
-- Michael Radermacher, Ph.D., Prof. University of Vermont Dept. Molecular Physiology and Biophysics HSRF 120 / 149 Beaumont Ave Burlington, VT 05405 USA
==============================Original Headers============================== 8, 25 -- From mraderma-at-uvm.edu Tue Jul 26 22:50:39 2011 8, 25 -- Received: from smtp1.uvm.edu (smtp1.uvm.edu [132.198.101.168]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6R3od12025429 8, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Jul 2011 22:50:39 -0500 8, 25 -- Received: from uvm.edu (penguin6.uvm.edu [132.198.101.216]) 8, 25 -- by smtp1.uvm.edu (8.14.2/8.14.2) with ESMTP id p6R3odIC018127 8, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 26 Jul 2011 23:50:39 -0400 8, 25 -- Received: from pool-71-254-9-179.burl.east.myfairpoint.net 8, 25 -- (pool-71-254-9-179.burl.east.myfairpoint.net [71.254.9.179]) by 8, 25 -- webmail.uvm.edu (Horde MIME library) with HTTP; Tue, 26 Jul 2011 23:50:39 8, 25 -- -0400 8, 25 -- Message-ID: {20110726235039.5puq6lrgz4u0wss4-at-webmail.uvm.edu} 8, 25 -- Date: Tue, 26 Jul 2011 23:50:39 -0400 8, 25 -- From: Michael Radermacher {mraderma-at-uvm.edu} 8, 25 -- To: Microscopy-at-microscopy.com 8, 25 -- Subject: Sunday short course on 3D EM at M&M meeting 2011 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Type: text/plain; 8, 25 -- charset=ISO-8859-1; 8, 25 -- DelSp="Yes"; 8, 25 -- format="flowed" 8, 25 -- Content-Disposition: inline 8, 25 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.4) 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6R3od12025429 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dzhou-at-tcd.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dzhou-at-tcd.ie Name: Dan
Organization: TCD & CRANN, Ireland
Title-Subject: [Filtered] TEM: EELS alingment or GIF CCD problem?
Message: Hi, all.
I recently really confused by our EELS system here. Anyone can help me to figure out if it is my alingment or the GIF CCD (or the lenses for EELS) damaged? Following is the procedure I did and the phenomenon I observed: 1 I align ZLP and tune GIF CCD in imaging mode. Check the image and observed image quite like overfocused carbon image while I illuminate a hole area without anything there. 2 Didn't met that before and didn't think it as abnormal at that stage. 3 Go to spectroscopy mode. The zero loss peak's FWHM is double the value I checked one day before, but the alignmetn file is the same, nobody use it between. Play a bit with 'focus x' and 'focus y', the energy resolution goes to the value I expected. To this stage, the zero loss peak looks OK. 4 But when I set offset to 500 eV, still hole area, and acquire spectrum with relative long time without saturate the CCD, it shows irregular wave with 3-5eV peak continuously. 5 Go back to imaging mode,prepare gain reference, align ZLP, tune GIF, and go back to spectroscopy mode again. The spectrum I got is still with irregular wave peaks, which don't belong to the sample energy loss. Thanks in advance. Dan
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gary-at-gaugler.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gary-at-gaugler.com Name: gary gaugler
Title-Subject: [Filtered] Microscopy errors
Message: This is of course very interesting. But I have a totally different experience that was and has been exasperating.
Filing the PTO Form VA for copyright registration of SEM, LM and TEM pix, the application was rejected....not once but several times. VA is visual arts--photographs. Well, the examiner rejected the application the first time because an image from a SEM or TEM was not a photograph. In his view, it was the product of a method of producing images and hence, was a patent issue. Sigh. There was no optical glass lens. There was no film (for SEM) but a negative for TEM.
The explanation I gave about how a TEM and SEM "images" were formed based on scanned or transmitted electrons did not connect with visual arts. Too technical of an explanation of how SEMs and TEMs work--oops. Electron optics was a deal killer.
Re-submitted and hope to get a different person. Nope. OK. Change the submission title. Re-submit. Now waiting for several months. Patents are backed-up too as I hear.
An objective lens made from copper wire? That did not fly. No shutter or flap mirror. No viewing prism. Registration denied. Finally, sanity should prevail.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lisa.whitworth-at-okstate.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: lisa.whitworth-at-okstate.edu Name: Lisa Whitworth
Organization: Oklahoma State University
Title-Subject: [Filtered] insect ovaries
Message: Hello, I am having a difficult time infiltrating ovaries from hawk moths. I tried Spurr's and Poly Bed 812 with 3:1 propylene oxide for up to 7 days, then 1:1, then 100% resin. I have not found much reference material, but 2 of the references I have found cite the use of these resins, but without much detail. One reference suggests LR White polymerized at 50oC under nitrogen.
If anyone has any experience with this and can give me some advice, I would greatly appreciate it!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kcildavis02-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kcildavis02-at-gmail.com Name: Christie Davis
Title-Subject: [Filtered] Epon Thick Sections Are Rolling-up
Message: Does anyone have suggestions on how to get epon thick sections (4 microns) to stop rolling up? We've played with the water level and the sectioning speed, but they still roll. Thank you!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ttxu-at-uncc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ttxu-at-uncc.edu Name: Terry Xu
Organization: UNC Charlotte
Title-Subject: [Filtered] TEM_Postdoctoral-level Electron Microscopist Position Available
Message: The University of North Carolina at Charlotte (UNC Charlotte) is seeking a postdoctoral-level electron microscopist to lead the operation and the user program of the Transmission Electron Microscopy lab. The start date of this position is January 2012.
For details, please visit http://coefs.uncc.edu/ttxu/position-opening/
Candidates please email applications to Dr. Terry Xu (ttxu-at-uncc.edu).
4 microns is a pretty thick Epon section. Is there a reason you don't want to do 1 micron which is more standard?
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
As mentioned in another reply the standard is about 1 micron.
It is difficult to get anything over 2 microns to stick to a slide during staining. You will not get good stain all the way through 4 microns.
Let us know more about your project . Why are you attempting 4 microns?
Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kcildavis02-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kcildavis02-at-gmail.com Name: Christie Davis
Title-Subject: [Filtered] Epon Thick Sections Are Rolling-up
Message: Does anyone have suggestions on how to get epon thick sections (4 microns) to stop rolling up? We've played with the water level and the sectioning speed, but they still roll. Thank you!
Sounds like a project that would be better suited for paraffin.
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
X-from: Christine Davis [mailto:kcildavis02-at-gmail.com] Sent: Wednesday, July 27, 2011 3:30 PM To: Phillips, Thomas E.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both marcela.redigolo-at-mail.wvu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV
Message: Dear all:
We have one user that prepares his biological tissues in resin and then cut them for TEM studies. He has been using a 80KV TEM for that, but that TEM does't have digital camera and he wants to start using ours.
We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do some imaging while playing a bit with the settings of the scope, but still in some cases the sample ends up burning. I'm not experienced with biological samples.
Is there a different sample preparation he should look for, or maybe change his resin or do cryo-cut, before coming to our scope, or is there a certain group of settings I should work with for this particular case? Thanks for any help.
By burning do you mean bubbling or exploding sections? Sometimes a thin carbon coating of the section will improve things greatly, even if the sections are on carbon coated formvar or lacey film supports. I can use a 10 nm STEM probe in a 2100F for EDS linescans and maps of biological sections-- if carbon coated. Without the carbon coating the films can explode under the beam.
In TEM mode I tend to use spot sizes 3-5 to minimize beam exposure/damage.
I do not believe a cryo cut would be of any value. A small objective aperture will help prevent/balance charging effects. How thick are the sections? I generally find 150-250 nm quite fine.
I grew up in the Materials world and now manage a structural biology TEM facility. HAADF STEM can be a great way to image biological low contrast samples.
Best regards, Roseann
Roseann Csencsits, PhD Manager Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Jul 27, 2011, at 3:03 PM, microscopylistserver-noreply-at-microscopy.com wrote:
} Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo } } Organization: WVU SRF } } Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV } } Message: Dear all: } } We have one user that prepares his biological tissues in resin and then } cut them for TEM studies. He has been using a 80KV TEM for that, but } that TEM does't have digital camera and he wants to start using ours. } } We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do } some imaging while playing a bit with the settings of the scope, but } still in some cases the sample ends up burning. I'm not experienced with } biological samples. } } Is there a different sample preparation he should look for, or maybe } change his resin or do cryo-cut, before coming to our scope, or is there } a certain group of settings I should work with for this particular case? } Thanks for any help. } } Marcela. }
==============================Original Headers============================== 11, 31 -- From rcsencsits-at-lbl.gov Wed Jul 27 18:07:05 2011 11, 31 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6RN74J4008607 11, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2011 18:07:04 -0500 11, 31 -- X-Ironport-SBRS: 4.4 11, 31 -- X-IronPort-Anti-Spam-Filtered: true 11, 31 -- X-IronPort-Anti-Spam-Result: AtQBAIGZME7RVdW0kGdsb2JhbAA0AQEBAQIBEAQCKjEZDAwMCyAbFCYBBQEcCAUDFienHggUAQEBAQkJDQcUBCGIfKRsCp0dgz2CJF8Eh1eXaDyDJVc 11, 31 -- X-IronPort-AV: E=Sophos;i="4.67,279,1309762800"; 11, 31 -- d="scan'208";a="47078346" 11, 31 -- Received: from mail-yx0-f180.google.com ([209.85.213.180]) 11, 31 -- by ironport4.lbl.gov with ESMTP; 27 Jul 2011 16:07:03 -0700 11, 31 -- Received: by yxk30 with SMTP id 30so1711731yxk.39 11, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2011 16:07:03 -0700 (PDT) 11, 31 -- Received: by 10.142.187.3 with SMTP id k3mr183490wff.391.1311808022822; 11, 31 -- Wed, 27 Jul 2011 16:07:02 -0700 (PDT) 11, 31 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.226]) 11, 31 -- by mx.google.com with ESMTPS id t7sm192215wfl.14.2011.07.27.16.07.01 11, 31 -- (version=TLSv1/SSLv3 cipher=OTHER); 11, 31 -- Wed, 27 Jul 2011 16:07:01 -0700 (PDT) 11, 31 -- References: {201107272203.p6RM3EeY005695-at-ns.microscopy.com} 11, 31 -- In-Reply-To: {201107272203.p6RM3EeY005695-at-ns.microscopy.com} 11, 31 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 31 -- Content-Type: text/plain; charset=us-ascii 11, 31 -- Message-Id: {99155632-DF84-4683-AD78-A56A0FE069CD-at-lbl.gov} 11, 31 -- From: Roseann Csencsits {rcsencsits-at-lbl.gov} 11, 31 -- Subject: Re: Biosample in TEM 80 KV and 200 KV 11, 31 -- Date: Wed, 27 Jul 2011 16:06:59 -0700 11, 31 -- To: Microscopy-at-microscopy.com, marcela.redigolo-at-mail.wvu.edu 11, 31 -- X-Mailer: Apple Mail (2.1084) 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6RN74J4008607 ==============================End of - Headers==============================
Your 200kV microscope will work very well down at 80kV and when you return to 200kV it will regain its absolute stability quite quickly.
I had a similar incident with a client who also worried about changing from their 200kV JEOL. I had the client take test pictures of the gold lattice from 200kV down to 80kV in 20kV steps; she was 100% successful!
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV
Message: Dear all:
We have one user that prepares his biological tissues in resin and then cut them for TEM studies. He has been using a 80KV TEM for that, but that TEM does't have digital camera and he wants to start using ours.
We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do some imaging while playing a bit with the settings of the scope, but still in some cases the sample ends up burning. I'm not experienced with biological samples.
Is there a different sample preparation he should look for, or maybe change his resin or do cryo-cut, before coming to our scope, or is there a certain group of settings I should work with for this particular case? Thanks for any help.
since we know a little bit more about the basics of the original question (Thanks to Prof. Phillips's posting!), i.e. sponge containing loosely, and therefore only little distributed organic matter in a normal 1 µm semithin section) I would like to chime in...
For me there are several questions: ia) did the sections roll up at 1µm thickness too? ib) what is the current block face area you try to section?
iia) no question about 4 µm a little bit too thick IMO, iib) what about sectioning speed, use of diamond knife/glass knife, and esp. sectioning angle of knife/knives
iiia) since much blank resin seems to be contained in the block/section therefore - one should think about the chosen hardness of the resin (should be adapted to the hardness of sponge structure(s), are there calcified structures included or were samples decalcified prior to embedding?) iiib) what about penetration quality of resin into the sample?
iv) I have serially sectioned (in the "primitive times" of my dissertation) rat brain (+/- whole hypothalamus, at least up to 3x4 mm sectioning area) at 2 µm routinely for 2D-3D reconstruction (at that time using "ordinary" glass knives: neither a problem with sectioning or adhesion as well as staining afterwards with a "crude" 3 step LM staining method (chrome-alum-gallocyanin stain), but had to iva) adjust resin to really SOFT as well as (I was able to section 10-15 consecutive sections -at- 2µm with ONE glass knife) Oh, yes...BTW...for sure knowing about the differences between soft brain and {hard} sponge tissue.. but since with my brain samples the whole space of the third ventricle had to be included there also was much blank resin in the sections... ivb) to pretreat slides (subbing) with (if I recall this correctly) chrome-alum-gelatine (==} cf. http://stainsfile.info/StainsFile/prepare/adhesives/chromegelatin.htm ) for this task.
v) IMHO, the advantage of "more material" to be seen in a section by making the section thicker will go against "resolution" if you are going to view at higher mag. or document by micrographs.
vi) therefore either you have to accept the material included in the section is low in quantity, as well as morphological detail (suggest or compare e.g. with {hydra} samples??) and you have eventually to section serially with high quality OR you collect every say 10th section (or collecting randomly, following "stereological" = mathematical principles) or vii) you go better (as Tom pointed out) if you try another technique (e.g. EM-Fixation and Dehydration as usual, followed by paraffin embedding, thick sectioning, overview-staining and IF you need special LM or EM then on a respective, interesting location in the section, you could re-embed that thick section into resin without difficulties or use another resin, like (Kulzer)Technovit (c),TM), cf.: http://www.immunologie-labor.com/cellmarker_files/IET_tissue_05.pdf, KUHLMANN 2008...ok: no reference for 4µm resin thick sections but rather infos on microtoming issues).
As an alternative, try to find references for such a task [google: { 4 µm semithin resin sections } ] e.g. http://jcp.bmj.com/content/58/9/897.full J Clin Pathol 2005;58:897-903 doi:10.1136/jcp.2004.023788 How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies T Krenacs, E Bagdi, E Stelkovics, L Bereczki, L Krenacs
or also http://www.sciencedirect.com/science/article/pii/0047720679900086 Micron (1969) Volume 10, Issue 2, 1979, Pages 145-147 A rapid method for resectioning of semithin large epoxy sections for electron microscopy [6 µm sections!] W. I. Butcher, R. E. Schmidt, F. A. Elias and M. J. Hammond
Nevertheless, for your quite nasty task: all the best and good luck!
Regards, Wolfgang Muss SALZBURG-AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu] } Gesendet: Mittwoch, 27. Juli 2011 23:44 } An: Muß Wolfgang } Betreff: [Microscopy] Re: Epon Thick Sections Are Rolling-up } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Sounds like a project that would be better suited for paraffin. } } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } X-from: Christine Davis [mailto:kcildavis02-at-gmail.com] } Sent: Wednesday, July 27, 2011 3:30 PM } To: Phillips, Thomas E. } Subject: Re: Subject: [Filtered] Epon Thick Sections Are Rolling-up } } The researcher is looking at sponge which isn't very dense and they } don't see enough tissue at 1 micron. } } Thank you! } Christie } On Wed, Jul 27, 2011 at 3:41 PM, Phillips, Thomas E. } {PhillipsT-at-missouri.edu} wrote: } 4 microns is a pretty thick Epon section. Is there a reason you don't } want to do 1 micron which is more standard? } } Thomas E. Phillips, Ph.D } Professor of Biological Sciences } Director, Molecular Cytology Core } 2 Tucker Hall } University of Missouri } Columbia, MO 65211-7400 } 573-882-4712 (office) } 573-882-0123 (fax) } phillipst-at-missouri.edu } } http://www.biology.missouri.edu/faculty/phillips.html } http://www.biotech.missouri.edu/mcc/ } } } } ==============================Original Headers============================== } 14, 27 -- From PhillipsT-at-missouri.edu Wed Jul 27 16:40:59 2011 } 14, 27 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01- } umsystem-out.um.umsystem.edu [209.106.229.49]) } 14, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p6RLexSg007807 } 14, 27 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jul 2011 16:40:59 } -0500 } 14, 27 -- X-IronPort-Anti-Spam-Filtered: true } 14, 27 -- X-IronPort-Anti-Spam-Result: } AgkBAKOEME7Raua1/2dsb2JhbAA0AQEFgRQEARoEAQEgFBsDDxEYDgkBBRcMl3ePSHAHiQC } iApZ+iH8CgzuCJF8EhygvlQmHFQ } 14, 27 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) } 14, 27 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 27 Jul } 2011 16:40:59 -0500 } 14, 27 -- Received: from um-email05.um.umsystem.edu ([169.254.1.65]) by } 14, 27 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; } Wed, 27 Jul 2011 } 14, 27 -- 16:40:59 -0500 } 14, 27 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} } 14, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 14, 27 -- Date: Wed, 27 Jul 2011 16:40:58 -0500 } 14, 27 -- Subject: Subject: [Filtered] Epon Thick Sections Are Rolling- } up } 14, 27 -- Thread-Topic: Subject: [Filtered] Epon Thick Sections Are } Rolling-up } 14, 27 -- Thread-Index: AcxMm+zB3Ilc5chqQYuYSNDnfAqSXAACV83QAAAeDhA= } 14, 27 -- Message-ID: {616B99DC91A1D64A9BC81BA4326586DF290DE69099-at-UM- } EMAIL05.um.umsystem.edu} } 14, 27 -- Accept-Language: en-US } 14, 27 -- Content-Language: en-US } 14, 27 -- X-MS-Has-Attach: } 14, 27 -- X-MS-TNEF-Correlator: } 14, 27 -- acceptlanguage: en-US } 14, 27 -- Content-Type: text/plain; charset="iso-8859-1" } 14, 27 -- MIME-Version: 1.0 } 14, 27 -- Content-Transfer-Encoding: 8bit } 14, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p6RLexSg007807 } ==============================End of - Headers==============================
==============================Original Headers============================== 21, 40 -- From W.Muss-at-salk.at Thu Jul 28 05:30:03 2011 21, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 21, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6SAU27E017614 21, 40 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2011 05:30:02 -0500 21, 40 -- Received: from localhost (localhost [127.0.0.1]) 21, 40 -- by hermes.salk.at (Postfix) with ESMTP id A517BC389C 21, 40 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2011 12:29:59 +0200 (CEST) 21, 40 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 21, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 21, 40 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 21, 40 -- with ESMTP id sI4F28xvXGgp for {microscopy-at-microscopy.com} ; 21, 40 -- Thu, 28 Jul 2011 12:29:59 +0200 (CEST) 21, 40 -- Received: from N2EX199.lks.local (n2ex199.lks.local [192.168.13.199]) 21, 40 -- by hermes.salk.at (Postfix) with ESMTP id E9E27C388E 21, 40 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2011 12:29:58 +0200 (CEST) 21, 40 -- Received: from n1rz122.lksdom21.lks.local ([192.168.101.122]) 21, 40 -- by N2EX199.lks.local with ESMTP; 28 Jul 2011 12:30:47 +0200 21, 40 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.130]) by n1rz122.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 21, 40 -- Thu, 28 Jul 2011 12:29:58 +0200 21, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 40 -- Content-class: urn:content-classes:message 21, 40 -- MIME-Version: 1.0 21, 40 -- Content-Type: text/plain; 21, 40 -- charset="iso-8859-1" 21, 40 -- Subject: [Microscopy] Re: Epon Thick Sections Are Rolling-up [ sponge ==} sounds like a project better suited for paraffin ? 21, 40 -- Date: Thu, 28 Jul 2011 12:29:58 +0200 21, 40 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F32FF0-at-N1RZ116.lksdom21.lks.local} 21, 40 -- In-Reply-To: {201107272143.p6RLhh38012715-at-ns.microscopy.com} 21, 40 -- X-MS-Has-Attach: 21, 40 -- X-MS-TNEF-Correlator: 21, 40 -- Thread-Topic: [Microscopy] Re: Epon Thick Sections Are Rolling-up [ sponge ==} sounds like a project better suited for paraffin ? 21, 40 -- Thread-Index: AcxMpkaphpEUGz+6SuaI18EhGmZhTgAYvUrw 21, 40 -- References: {201107272143.p6RLhh38012715-at-ns.microscopy.com} 21, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 21, 40 -- To: {kcildavis02-at-gmail.com} 21, 40 -- Cc: {microscopy-at-microscopy.com} 21, 40 -- X-OriginalArrivalTime: 28 Jul 2011 10:29:58.0735 (UTC) FILETIME=[4CEDFDF0:01CC4D11] 21, 40 -- X-Scanned-By: SALK-Content-Filter 21, 40 -- Content-Transfer-Encoding: 8bit 21, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6SAU27E017614 ==============================End of - Headers==============================
Just to add what may be an obvious note about operating at a lower accelerating voltage. If the 2100 has never been aligned at anything other than 200kV, you may need to have a service engineer in to do initial alignments at lower voltages. When our JEOL JEM-3010 was installed, the engineers did alignments at 50kV intervals from 50 to 300kV and stored those values for future use. On the rare occasions when I use a lower accelerating voltage, I just have to do some minor touch-up to the alignment. Also, you'll want to check your magnification calibration at the lower accelerating voltage.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. ********************************************************************* -----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Thursday, July 28, 2011 3:35 AM To: Elaine F. Schumacher
Dear Marcela
Your 200kV microscope will work very well down at 80kV and when you return to 200kV it will regain its absolute stability quite quickly.
I had a similar incident with a client who also worried about changing from their 200kV JEOL. I had the client take test pictures of the gold lattice from 200kV down to 80kV in 20kV steps; she was 100% successful!
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV
Message: Dear all:
We have one user that prepares his biological tissues in resin and then cut them for TEM studies. He has been using a 80KV TEM for that, but that TEM does't have digital camera and he wants to start using ours.
We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do some imaging while playing a bit with the settings of the scope, but still in some cases the sample ends up burning. I'm not experienced with biological samples.
Is there a different sample preparation he should look for, or maybe change his resin or do cryo-cut, before coming to our scope, or is there a certain group of settings I should work with for this particular case? Thanks for any help.
Hi Marcela, I also needed to adapt biological sections for use in a 200 KV TEM. I routinely purchase carbon/formvar-coated copper grids (200 mesh) and collect the sections on the 'light' non-carbon side of the grid (less phobic and easier to collect sections from the boat water). The stability of the carbon plus support film permits the use of thinner sections (less than 70 nm) and there is less drift of the material under the electron beam. The electron beam can be focused to a fine spot on these grids for making height adjustment and for x-ray analysis. I did not like carbon-only coated grids because of the increased background texture.
I stopped trying to make these grids myself - the time involved and inconsistent quality of my homemade grids were not worth it. The increased grid cost is offset by your reduced photography cost.
Changing KV to 120 in the instrument required additional magnification calibrations and although this may improve contrast in the biological material on the screen, the digital camera we use provides excellent contrast in the final image. So depending on the camera and your final images, a lower KV may improve contrast but I would definitely recommend carbon/formvar-coated grids.
Regards, Susan Belfry
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Marcela,
By burning do you mean bubbling or exploding sections? Sometimes a thin carbon coating of the section will improve things greatly, even if the sections are on carbon coated formvar or lacey film supports. I can use a 10 nm STEM probe in a 2100F for EDS linescans and maps of biological sections-- if carbon coated. Without the carbon coating the films can explode under the beam. In TEM mode I tend to use spot sizes 3-5 to minimize beam exposure/damage.
I do not believe a cryo cut would be of any value. A small objective aperture will help prevent/balance charging effects. How thick are the sections? I generally find 150-250 nm quite fine.
I grew up in the Materials world and now manage a structural biology TEM facility. HAADF STEM can be a great way to image biological low contrast samples.
Best regards, Roseann
Roseann Csencsits, PhD Manager Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Jul 27, 2011, at 3:03 PM, microscopylistserver-noreply-at-microscopy.com wrote:
} Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo } } Organization: WVU SRF } } Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV } } Message: Dear all: } } We have one user that prepares his biological tissues in resin and then } cut them for TEM studies. He has been using a 80KV TEM for that, but } that TEM does't have digital camera and he wants to start using ours. } } We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do } some imaging while playing a bit with the settings of the scope, but } still in some cases the sample ends up burning. I'm not experienced with } biological samples. } } Is there a different sample preparation he should look for, or maybe } change his resin or do cryo-cut, before coming to our scope, or is there } a certain group of settings I should work with for this particular case? } Thanks for any help. } } Marcela. }
==============================Original Headers============================== 11, 31 -- From rcsencsits-at-lbl.gov Wed Jul 27 18:07:05 2011 11, 31 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6RN74J4008607 11, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2011 18:07:04 -0500 11, 31 -- X-Ironport-SBRS: 4.4 11, 31 -- X-IronPort-Anti-Spam-Filtered: true 11, 31 -- X-IronPort-Anti-Spam-Result: AtQBAIGZME7RVdW0kGdsb2JhbAA0AQEBAQIBEAQCKjEZDAwMCyAbFCYBBQEcCAUDFienHggUAQEBAQkJDQcUBCGIfKRsCp0dgz2CJF8Eh1eXaDyDJVc 11, 31 -- X-IronPort-AV: E=Sophos;i="4.67,279,1309762800"; 11, 31 -- d="scan'208";a="47078346" 11, 31 -- Received: from mail-yx0-f180.google.com ([209.85.213.180]) 11, 31 -- by ironport4.lbl.gov with ESMTP; 27 Jul 2011 16:07:03 -0700 11, 31 -- Received: by yxk30 with SMTP id 30so1711731yxk.39 11, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 27 Jul 2011 16:07:03 -0700 (PDT) 11, 31 -- Received: by 10.142.187.3 with SMTP id k3mr183490wff.391.1311808022822; 11, 31 -- Wed, 27 Jul 2011 16:07:02 -0700 (PDT) 11, 31 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov [131.243.35.226]) 11, 31 -- by mx.google.com with ESMTPS id t7sm192215wfl.14.2011.07.27.16.07.01 11, 31 -- (version=TLSv1/SSLv3 cipher=OTHER); 11, 31 -- Wed, 27 Jul 2011 16:07:01 -0700 (PDT) 11, 31 -- References: {201107272203.p6RM3EeY005695-at-ns.microscopy.com} 11, 31 -- In-Reply-To: {201107272203.p6RM3EeY005695-at-ns.microscopy.com} 11, 31 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 31 -- Content-Type: text/plain; charset=us-ascii 11, 31 -- Message-Id: {99155632-DF84-4683-AD78-A56A0FE069CD-at-lbl.gov} 11, 31 -- From: Roseann Csencsits {rcsencsits-at-lbl.gov} 11, 31 -- Subject: Re: Biosample in TEM 80 KV and 200 KV 11, 31 -- Date: Wed, 27 Jul 2011 16:06:59 -0700 11, 31 -- To: Microscopy-at-microscopy.com, marcela.redigolo-at-mail.wvu.edu 11, 31 -- X-Mailer: Apple Mail (2.1084) 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6RN74J4008607 ==============================End of - Headers==============================
==============================Original Headers============================== 17, 16 -- From belfry-at-unb.ca Thu Jul 28 08:10:59 2011 17, 16 -- Received: from tempo.its.unb.ca (tempo.its.unb.ca [131.202.1.21]) 17, 16 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6SDAxBb019622 17, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2011 08:10:59 -0500 17, 16 -- Received: from [127.0.0.1] (mmf.biology.unb.ca [131.202.94.20]) 17, 16 -- by tempo.its.unb.ca (8.13.8/8.13.8) with ESMTP id p6SDAxIE014156 17, 16 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jul 2011 10:10:59 -0300 17, 16 -- Message-ID: {4E315FD7.8040305-at-unb.ca} 17, 16 -- Date: Thu, 28 Jul 2011 10:10:47 -0300 17, 16 -- From: Susan Belfry {belfry-at-unb.ca} 17, 16 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.18) Gecko/20110616 Thunderbird/3.1.11 17, 16 -- MIME-Version: 1.0 17, 16 -- To: microscopy-at-microscopy.com 17, 16 -- Subject: [Microscopy] Re: Biosample in TEM 80 KV and 200 KV 17, 16 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 17, 16 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Thanks for all the replies and many suggestions. We indeed never worked with the scope at 80KV and you are correct, Elaine. The alignment is way off. Still, I'll keep this idea in mind. It will be very helpful to have the scope operating on both accelerating voltages. But before doing this, I'll try many other ideas that were suggested for this biosample in particular.
Thanks again to all for your replies. I really appreciate!
Kindest regards,
Marcela.
} } } 07/28/11 9:01 AM } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello Steve and Fellow Listers,
Just to add what may be an obvious note about operating at a lower accelerating voltage. If the 2100 has never been aligned at anything other than 200kV, you may need to have a service engineer in to do initial alignments at lower voltages. When our JEOL JEM-3010 was installed, the engineers did alignments at 50kV intervals from 50 to 300kV and stored those values for future use. On the rare occasions when I use a lower accelerating voltage, I just have to do some minor touch-up to the alignment. Also, you'll want to check your magnification calibration at the lower accelerating voltage.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. ********************************************************************* -----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Thursday, July 28, 2011 3:35 AM To: Elaine F. Schumacher
Dear Marcela
Your 200kV microscope will work very well down at 80kV and when you return to 200kV it will regain its absolute stability quite quickly.
I had a similar incident with a client who also worried about changing from their 200kV JEOL. I had the client take test pictures of the gold lattice from 200kV down to 80kV in 20kV steps; she was 100% successful!
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV
Message: Dear all:
We have one user that prepares his biological tissues in resin and then cut them for TEM studies. He has been using a 80KV TEM for that, but that TEM does't have digital camera and he wants to start using ours.
We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do some imaging while playing a bit with the settings of the scope, but still in some cases the sample ends up burning. I'm not experienced with biological samples.
Is there a different sample preparation he should look for, or maybe change his resin or do cryo-cut, before coming to our scope, or is there a certain group of settings I should work with for this particular case? Thanks for any help.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kcildavis02-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kcildavis02-at-gmail.com Name: Christie Davis
Title-Subject: [Filtered] Epon Thick Sections Are Rolling-up
Message: Thank you for your suggestions and patience as I learn to use the list server! HereÂ’s the project info so far as IÂ’ve learned: The material is sponge with spicules, the researcher does want 4-5 micron thick sections, and we use a histo diamond knife. We trim to 2 mm size trapezoids. The lab has been doing this for a few years, but the two students who mainly work on the project are away this summer and the person who supervised them left the institution. We also bought a new epon kit and theyÂ’re epon recipe doesnÂ’t allow for the WPE change as mine does so the block composition has possibly changed. I think itÂ’s softer. IÂ’m teaching students who have never sectioned anything before and IÂ’ve never done such thick epon sections. The toluidine blue (2.9% w/v) with borax (2.7% w/v) penetrates the entire 5 microns without a problem. Pre-warmed Superfrosted slides must be used to hold the sections and we wash with 95%Etoh then 50% Etoh then water. They seem to stick fine, but you must be careful on the water step. The sections tend to only curve slightly when I do it by hand or when I play with the water and speed to get it just right. I think experience will help the students some. They have done extremely large square block faces and 5-micron sections in the past (over 4mm), but have blown through the histo knife. When asked, I suggested going to 4 microns and smaller block faces to help stretch the knife life. So as you can see a lot has changed in the project so pinpointing the solution might be difficult. The solution might also just be experience since I can cope better than the students. I never got to try the old epon, but I suspect that change is important also. Does anyone think the square shape would be better? Thank you again and I hope the details help.
We are a long time user of Gatan Digital Micrograph (DM), including several plug-ins that are available for it. We did install a new ccd camera from Tvips in one of our TEMs, and obviously it uses a different image file format. It is a kind of proprietary 16 bit tiff with a 512 bit header. We are able to open the tiff image in the DM, but the calibration is lost. We can extract the calibration from the tiff with another program and fix calibration of the image in the DM. The problem is when you have a batch of images and it is a pain to do this task manually. With no calibration the image is useless.
I was wondering if someone has already solved this problem and have a script to this task on DM. Open a Tvips tiff file and correct calibration. There is a script that does the opposite, batch convert DM3 files to standard tiff. I just don't want to reinvent the wheel. Before someone mentions, I already did took a look at the DMScripting webpage.
Thanks,
Carlos
__ Carlos Kazuo Inoki Laboratório Nacional de Nanotecnologia Caixa Postal 6192 Campinas, SP 13083-970 Brazil
==============================Original Headers============================== 6, 25 -- From carlos.inoki-at-lnls.br Thu Jul 28 09:49:15 2011 6, 25 -- Received: from correio.lnls.br (correio.lnls.br [200.133.194.7]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6SEnDfF004709 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jul 2011 09:49:14 -0500 6, 25 -- Received: from oberon.abtlus.org.br (unknown [10.0.0.18]) 6, 25 -- by correio.lnls.br (Postfix) with ESMTP id C3BE2DDA20 6, 25 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jul 2011 11:49:05 -0300 (BRT) 6, 25 -- Received: from oberon.abtlus.org.br ([10.0.0.18]) by oberon ([10.0.0.18]) with 6, 25 -- mapi; Thu, 28 Jul 2011 11:49:08 -0300 6, 25 -- From: Carlos Kazuo Inoki {carlos.inoki-at-lnls.br} 6, 25 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 6, 25 -- Date: Thu, 28 Jul 2011 11:49:08 -0300 6, 25 -- Subject: TEM: Tvips EM-Menu TIFF and Gatan Digital Micrograph DM3 6, 25 -- Thread-Topic: Tvips EM-Menu TIFF and Gatan Digital Micrograph DM3 6, 25 -- Thread-Index: AQHMTTLVnxXJSMfo/EKb4XGNOUa55g== 6, 25 -- Message-ID: {E8C8ED1A9F8E9641B9E548358677CCB0A046788856-at-oberon} 6, 25 -- Accept-Language: en-US, pt-BR 6, 25 -- Content-Language: en-US 6, 25 -- X-MS-Has-Attach: 6, 25 -- X-MS-TNEF-Correlator: 6, 25 -- acceptlanguage: en-US, pt-BR 6, 25 -- Content-Type: text/plain; charset="iso-8859-1" 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6SEnDfF004709 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both csown-at-beamdevices.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: csown-at-beamdevices.com Name: Chris Own
Organization: BeamDevices, Inc
Title-Subject: [Filtered] Looking for a used Digiscan I
Message: Hi,
I am interested in obtaining a used Gatan Digiscan I for interfacing with an SEM. I'm wondering if anybody on the listserv might have an extra one sitting around in a closet in their lab gathering dust that they'd be willing to part with. If you have one, please contact me.
Has anybody tried to download a copy of the program from the Cambridge Journals web site? It displays on line as a Flash publication. There is an off-line version that can be downloaded, but it does not work on an ipad. There is a help menu that says any page range can be printed, but when I try to print it, it only allows 15 pages at a time to be printed, which is a pain because there are over 200 pages, and Flash crashes every time anyway when i try to print to PDF. What I really need it is PDF so I can load it onto my iPad and/or iPhone. Does anyone know how do do this? Is Cambridge going to post a more useful version? I am not going to put it on my laptop because my laptop weighs more than the printed version. Can we save some trees here?
John Mardinly ASU
==============================Original Headers============================== 2, 36 -- From John.Mardinly-at-asu.edu Fri Jul 29 01:41:09 2011 2, 36 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6T6f9E6015742 2, 36 -- for {Microscopy-at-Microscopy.com} ; Fri, 29 Jul 2011 01:41:09 -0500 2, 36 -- X-ASG-Debug-ID: 1311921668-759f60fe0001-FOsErg 2, 36 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id SX8QxWtpkw50aM5y (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Thu, 28 Jul 2011 23:41:08 -0700 (MST) 2, 36 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 2, 36 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 2, 36 -- X-ASG-Whitelist: Client 2, 36 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.207) by 2, 36 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 2, 36 -- 8.2.255.0; Thu, 28 Jul 2011 23:41:08 -0700 2, 36 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 2, 36 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 28 Jul 2, 36 -- 2011 23:41:08 -0700 2, 36 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 2, 36 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Fri, 29 Jul 2011 2, 36 -- 06:41:08 +0000 2, 36 -- Subject: M and M 2011 Program 2, 36 -- From: John Mardinly {John.Mardinly-at-asu.edu} 2, 36 -- X-ASG-Orig-Subj: M and M 2011 Program 2, 36 -- Content-Type: text/plain; charset="us-ascii" 2, 36 -- thread-topic: M and M 2011 Program 2, 36 -- Message-ID: {BF4BBE32-3AC5-4959-9AE6-55B2F14EFBAE-at-exchange.asu.edu} 2, 36 -- thread-index: AcxNun8z/aL5/rUNQ1um6juwdlyZOg== 2, 36 -- Date: Thu, 28 Jul 2011 23:44:10 -0700 2, 36 -- To: MSA Listserver {Microscopy-at-Microscopy.com} 2, 36 -- MIME-Version: 1.0 (iPad Mail 8G4) 2, 36 -- X-OriginalArrivalTime: 29 Jul 2011 06:41:08.0824 (UTC) FILETIME=[7FAD9580:01CC4DBA] 2, 36 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 2, 36 -- X-Barracuda-Start-Time: 1311921668 2, 36 -- X-Barracuda-Encrypted: RC4-MD5 2, 36 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 2, 36 -- X-Virus-Scanned: by bsmtpd at asu.edu 2, 36 -- Content-Transfer-Encoding: 8bit 2, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6T6f9E6015742 ==============================End of - Headers==============================
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
-----Original Message----- X-from: Phillips, Thomas E. [mailto:PhillipsT-at-missouri.edu] Sent: Thursday, July 21, 2011 4:29 PM To: jmircheski-at-us.es; microscopy-at-microscopy.com
Dear Listers,
I need to etch some Epon from my blocks, and I intended using Sodium Ethoxide for that. Unfortunately, I can't find the detailed protocol that I used (only once) several years ago. Can anyone provide a detailed protocol for the preparation, please? Thanks,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
Thank you very much for your advices and help. I tried to image epon block faces, but without success. We used two microscopes, a JEOL 6460LV and a higher resolution Hitachi S5200. There were no environmental SEM nor a SEM with a microtome available. We used coated and uncoated blocks, combined with etched and unetched resin. The result was always the same - zero, or close to zero. Now, after re-reading your messages, I see that many of you (Derrick, Zack, Ed...) suggest coating with CARBON, and we coated with GOLD. Two reasons for our choice of gold: the carbon coater is broken, and the SEM technician has little experience in biological samples (I have 0 experience in SEM) and assumed that it will be fine with the gold. Well, now I have to wait until September for people to come back from vacations, and don't know till when until the carbon coater is repaired... Then maybe I can tell you if it was successful.
The question: what do you think, was the gold the reason that we couldn't see any fibres at the block surface? Will carbon alleviate the situation?
And one additional, maybe worthy of another topic: the intention of the "epon in SEM" was to check these blocks under SEM, so that we can use them later in a FIB/SEM. Is there anyone experienced in FIB/SEM? If so, have you ever tried to use epon blocks/sections?
Thanks again to everyone.
Regards from Sevilla, I'm freezing here (it's only 32ºC)
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
-----Original Message----- X-from: Haller, Edward [mailto:ehaller-at-health.usf.edu] Sent: Thursday, July 21, 2011 9:19 PM To: jmircheski-at-us.es; Microscopy-at-microscopy.com
One more question for today: Has anyone examined EPON blocks in a SEM? I tried to image my muscle samples embedded in EPON with a SEM. (the same block was previously used for ultra-thin sections, so its surface was pretty flat). The only thing I could see were brighter than background spots representing the muscle fibres. I'll try to remove some resin from the surface with sodium ethoxide, so that only the sample "sticks out" a little bit over the resin, unembedded. Has anyone else done something similar (seeing EPON blocks in SEM)? Could you please share your experience?
Thanks again,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
I agree...let's make this user friendly with modern communication devices. -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} } Reply-To: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} } Date: Fri, 29 Jul 2011 02:42:50 -0400 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] M and M 2011 Program } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Has anybody tried to download a copy of the program from the Cambridge } Journals web site? It displays on line as a Flash publication. There is an } off-line version that can be downloaded, but it does not work on an ipad. } There is a help menu that says any page range can be printed, but when I try } to print it, it only allows 15 pages at a time to be printed, which is a pain } because there are over 200 pages, and Flash crashes every time anyway when i } try to print to PDF. What I really need it is PDF so I can load it onto my } iPad and/or iPhone. Does anyone know how do do this? Is Cambridge going to } post a more useful version? I am not going to put it on my laptop because my } laptop weighs more than the printed version. Can we save some trees here? } } John Mardinly } ASU } } ==============================Original Headers============================== } 2, 36 -- From John.Mardinly-at-asu.edu Fri Jul 29 01:41:09 2011 } 2, 36 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) } 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p6T6f9E6015742 } 2, 36 -- for {Microscopy-at-Microscopy.com} ; Fri, 29 Jul 2011 01:41:09 -0500 } 2, 36 -- X-ASG-Debug-ID: 1311921668-759f60fe0001-FOsErg } 2, 36 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu } [129.219.103.59]) by bcnet6.asu.edu with ESMTP id SX8QxWtpkw50aM5y } (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO) for } {Microscopy-at-Microscopy.com} ; Thu, 28 Jul 2011 23:41:08 -0700 (MST) } 2, 36 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 2, 36 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 } 2, 36 -- X-ASG-Whitelist: Client } 2, 36 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.207) by } 2, 36 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP } Server id } 2, 36 -- 8.2.255.0; Thu, 28 Jul 2011 23:41:08 -0700 } 2, 36 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by } 2, 36 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, } 28 Jul } 2, 36 -- 2011 23:41:08 -0700 } 2, 36 -- Received: from 129.219.12.225 ([129.219.12.225]) by } EX05.asurite.ad.asu.edu } 2, 36 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Fri, 29 } Jul 2011 } 2, 36 -- 06:41:08 +0000 } 2, 36 -- Subject: M and M 2011 Program } 2, 36 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 2, 36 -- X-ASG-Orig-Subj: M and M 2011 Program } 2, 36 -- Content-Type: text/plain; charset="us-ascii" } 2, 36 -- thread-topic: M and M 2011 Program } 2, 36 -- Message-ID: {BF4BBE32-3AC5-4959-9AE6-55B2F14EFBAE-at-exchange.asu.edu} } 2, 36 -- thread-index: AcxNun8z/aL5/rUNQ1um6juwdlyZOg== } 2, 36 -- Date: Thu, 28 Jul 2011 23:44:10 -0700 } 2, 36 -- To: MSA Listserver {Microscopy-at-Microscopy.com} } 2, 36 -- MIME-Version: 1.0 (iPad Mail 8G4) } 2, 36 -- X-OriginalArrivalTime: 29 Jul 2011 06:41:08.0824 (UTC) } FILETIME=[7FAD9580:01CC4DBA] } 2, 36 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] } 2, 36 -- X-Barracuda-Start-Time: 1311921668 } 2, 36 -- X-Barracuda-Encrypted: RC4-MD5 } 2, 36 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi } 2, 36 -- X-Virus-Scanned: by bsmtpd at asu.edu } 2, 36 -- Content-Transfer-Encoding: 8bit } 2, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p6T6f9E6015742 } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 31 -- From dsherman-at-purdue.edu Fri Jul 29 07:43:25 2011 5, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 5, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6TChO2K021926 5, 31 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2011 07:43:24 -0500 5, 31 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 5, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p6TChOJJ010459 5, 31 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2011 08:43:24 -0400 5, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.217]) by WPPEXHUB04H.purdue.lcl 5, 31 -- ([::1]) with mapi; Fri, 29 Jul 2011 08:43:24 -0400 5, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 5, 31 -- To: "John.Mardinly-at-asu.edu" {John.Mardinly-at-asu.edu} , 5, 31 -- "message to: MSA list" 5, 31 -- {microscopy-at-microscopy.com} 5, 31 -- Date: Fri, 29 Jul 2011 08:43:22 -0400 5, 31 -- Subject: Re: [Microscopy] M and M 2011 Program 5, 31 -- Thread-Topic: [Microscopy] M and M 2011 Program 5, 31 -- Thread-Index: AcxNury0FbtE7TJ8RsK8UlLAFKbZcAAMlz03 5, 31 -- Message-ID: {CA58232A.11D3D%dsherman-at-purdue.edu} 5, 31 -- In-Reply-To: {201107290642.p6T6goIH018611-at-ns.microscopy.com} 5, 31 -- Accept-Language: en-US 5, 31 -- Content-Language: en-US 5, 31 -- X-MS-Has-Attach: 5, 31 -- X-MS-TNEF-Correlator: 5, 31 -- user-agent: Microsoft-Entourage/13.10.0.110428 5, 31 -- acceptlanguage: en-US 5, 31 -- Content-Type: text/plain; charset="us-ascii" 5, 31 -- MIME-Version: 1.0 5, 31 -- X-PMX-Version: 5.5.9.388399 5, 31 -- X-PerlMx-Virus-Scanned: Yes 5, 31 -- Content-Transfer-Encoding: 8bit 5, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6TChO2K021926 ==============================End of - Headers==============================
I have contacted Cambridge about this a few days ago as I discovered the same issues with the Flash version of EXPO.
Cambridge has promised a full PDF downloadable version on-line in a few days. Hopefully it will be there by this coming Monday (7/1).
Nestor Your Friendly Neighborhood SysOp & President - Microscopy Society of America
-------- Original Message --------
Has anybody tried to download a copy of the program from the Cambridge Journals web site? It displays on line as a Flash publication. There is an off-line version that can be downloaded, but it does not work on an ipad. There is a help menu that says any page range can be printed, but when I try to print it, it only allows 15 pages at a time to be printed, which is a pain because there are over 200 pages, and Flash crashes every time anyway when i try to print to PDF. What I really need it is PDF so I can load it onto my iPad and/or iPhone. Does anyone know how do do this? Is Cambridge going to post a more useful version? I am not going to put it on my laptop because my laptop weighs more than the printed version. Can we save some trees here?
John Mardinly ASU
==============================Original Headers============================== 2, 36 -- From John.Mardinly-at-asu.edu Fri Jul 29 01:41:09 2011 2, 36 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6T6f9E6015742 2, 36 -- for {Microscopy-at-Microscopy.com} ; Fri, 29 Jul 2011 01:41:09 -0500 2, 36 -- X-ASG-Debug-ID: 1311921668-759f60fe0001-FOsErg 2, 36 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id SX8QxWtpkw50aM5y (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Thu, 28 Jul 2011 23:41:08 -0700 (MST) 2, 36 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 2, 36 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 2, 36 -- X-ASG-Whitelist: Client 2, 36 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.207) by 2, 36 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 2, 36 -- 8.2.255.0; Thu, 28 Jul 2011 23:41:08 -0700 2, 36 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 2, 36 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 28 Jul 2, 36 -- 2011 23:41:08 -0700 2, 36 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 2, 36 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Fri, 29 Jul 2011 2, 36 -- 06:41:08 +0000 2, 36 -- Subject: [Filtered] M and M 2011 Program 2, 36 -- From: John Mardinly {John.Mardinly-at-asu.edu} 2, 36 -- X-ASG-Orig-Subj: M and M 2011 Program 2, 36 -- Content-Type: text/plain; charset="us-ascii" 2, 36 -- thread-topic: M and M 2011 Program 2, 36 -- Message-ID: {BF4BBE32-3AC5-4959-9AE6-55B2F14EFBAE-at-exchange.asu.edu} 2, 36 -- thread-index: AcxNun8z/aL5/rUNQ1um6juwdlyZOg== 2, 36 -- Date: Thu, 28 Jul 2011 23:44:10 -0700 2, 36 -- To: MSA Listserver {Microscopy-at-Microscopy.com} 2, 36 -- MIME-Version: 1.0 (iPad Mail 8G4) 2, 36 -- X-OriginalArrivalTime: 29 Jul 2011 06:41:08.0824 (UTC) FILETIME=[7FAD9580:01CC4DBA] 2, 36 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 2, 36 -- X-Barracuda-Start-Time: 1311921668 2, 36 -- X-Barracuda-Encrypted: RC4-MD5 2, 36 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 2, 36 -- X-Virus-Scanned: by bsmtpd at asu.edu 2, 36 -- Content-Transfer-Encoding: 8bit 2, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6T6f9E6015742 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 28 -- From zaluzec-at-aaem.amc.anl.gov Fri Jul 29 08:00:03 2011 18, 28 -- Received: from znl.com ([206.69.208.20]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6TD031K017175 18, 28 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2011 08:00:03 -0500 18, 28 -- Received: from localhost (localhost [127.0.0.1]) 18, 28 -- by znl.com (Postfix) with ESMTP id 0CA913E76F1; 18, 28 -- Fri, 29 Jul 2011 08:00:03 -0500 (CDT) 18, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 18, 28 -- Received: from znl.com ([127.0.0.1]) 18, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 18, 28 -- with ESMTP id H7lh9swfPzIf; Fri, 29 Jul 2011 08:00:00 -0500 (CDT) 18, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 18, 28 -- by znl.com (Postfix) with ESMTPA id 06B5D3E76E5; 18, 28 -- Fri, 29 Jul 2011 08:00:00 -0500 (CDT) 18, 28 -- Message-ID: {4E32AECF.9050401-at-aaem.amc.anl.gov} 18, 28 -- Date: Fri, 29 Jul 2011 07:59:59 -0500 18, 28 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 18, 28 -- Organization: ANL 18, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 18, 28 -- MIME-Version: 1.0 18, 28 -- To: Aaron B Johnson {abjohnson-at-cambridge.org} , 18, 28 -- Janet Woodward {jhwoodward-at-buckman.com} , 18, 28 -- "Piston, Dave" {dave.piston-at-vanderbilt.edu} , 18, 28 -- MicroscopyListServer-Forward {microscopy-at-microscopy.com} , 18, 28 -- Morrell Gillette {mgillette-at-cambridge.org} 18, 28 -- Subject: Fwd: [Microscopy] M and M 2011 Program - PDF Version 18, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 18, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You can attempt to view your block using a low accelerating voltage and not coating the block if you have a field-emission SEM. With the Hitachi S5200, you might be able to image at a few kV in backscattered mode uncoated and not melt the epon. It is worth a try. As soon as you coat with gold, you will cover everything in the block, and will see nothing inside the block unless it has a higher atomic number than the gold itself, or unless it is physically sticking out of the surface of the block. With the FIB, although I don't use one, you may be able to preferentially etch away the epon to get some surface relief of the tissue, since the tissue is osmium fixed, it may be slightly harder than the plastic, and may resist etching a bit. I haven't tried this. If that's the case, then you could lightly coat with gold. You might have to explore etching at different angles to do this.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: Josif Mircheski [jmircheski-at-us.es] Sent: Friday, July 29, 2011 8:40 AM To: Haller, Edward; microscopy-at-microscopy.com
One more question for today: Has anyone examined EPON blocks in a SEM? I tried to image my muscle samples embedded in EPON with a SEM. (the same block was previously used for ultra-thin sections, so its surface was pretty flat). The only thing I could see were brighter than background spots representing the muscle fibres. I'll try to remove some resin from the surface with sodium ethoxide, so that only the sample "sticks out" a little bit over the resin, unembedded. Has anyone else done something similar (seeing EPON blocks in SEM)? Could you please share your experience?
Thanks again,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
Hi Nestor I haven't seen any comments about the on-line version.
It is excellent. They did a really good job. http://content.yudu.com/A1sxh8/MAMv17s1/resources/index.htm?referrerUrl=http%3A%2F%2Fjournals.cambridge.org%2Faction%2FdisplayJournal%3Fjid%3DMAM
The bookmarks and the search engine work very well.
Do you know what program they use to do this? Elaine
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Dr. Elaine C. Humphrey STEHM Technologist and Lab Manager Bob Wright Science Centre A015 Advanced Microscopy Facility University of Victoria, Canada
Hello Everyone, I'm using Pax-it software to capture images and make measurements on my stereomicroscope but I'm having trouble with calibrating the images.
If I take an image of a mm ruler, run the calibration routine on a distance of 5mm and then take the same image, and measure the same distance I get 4.5mm. Now I'm using the same side of the markers. I get this effect if I close the calibrated image and then reopen it. I've recalibrated, restarted the software and the computer and get the same results.
We did a Pax-on line conference, the service guy got to work, but as soon as we signed off and I repeated his step we were back to getting 4.5 or 4.8 for a actual 5mm span.
Any Pax-it users with suggestions?
Frank Karl Microscopist
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 9, 25 -- From frank_karl-at-ardl.com Fri Jul 29 12:01:35 2011 9, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6TH1YFk004113 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jul 2011 12:01:35 -0500 9, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 9, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 29 9, 25 -- Jul 2011 13:01:32 -0400 9, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 9, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 9, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 9, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 9, 25 -- Date: Fri, 29 Jul 2011 13:01:31 -0400 9, 25 -- Subject: pax-it software 9, 25 -- Thread-Topic: pax-it software 9, 25 -- Thread-Index: AcxOESoUDfRyDs9iTH2vjeEToOh2Pg== 9, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077D764956E-at-exchange2k7.ad.ardl.com} 9, 25 -- Accept-Language: en-US 9, 25 -- Content-Language: en-US 9, 25 -- X-MS-Has-Attach: 9, 25 -- X-MS-TNEF-Correlator: 9, 25 -- acceptlanguage: en-US 9, 25 -- Content-Type: text/plain; charset="iso-8859-1" 9, 25 -- MIME-Version: 1.0 9, 25 -- Content-Transfer-Encoding: 8bit 9, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p6TH1YFk004113 ==============================End of - Headers==============================
The on-line page turning version of EXPO is written/done using FLASH, and was done by the team at Cambridge University Press.
I concur it works well (even on my Desktop Mac).
Flash is not supported on a number of Apple OS platforms (iPad, iPhone ....) and since many of us carry portable HW to the meeting this is an issue. A downloadable PDF version will fix this issue.
Cambridge will have a full PDF version available for download shortly. When that happens we will post an update on the MM2011 & Journal WWW sites and I will also see to it that the Listserver subscribers are notified.
Just to confirm, all attendee's at M&M2011 will receive a printed copy of the EXPO. This will include a DVD of the Full M&M Proceedings with all the manuscripts. Electronic download of manuscripts will also be available on-line through the Journal Web site, after the meeting opens.
Lastly, as long as I'm churning out details, a hard copy of the Proceedings, for those of you like me who still like to have a printed book, is available via Print-on-Demand service. Details available on the Registration Page, at the Meeting or via Cambridge Press.
Hope to see many of you in about at week in Nashville, still plenty of time to register (Nestor takes off his M&M/MSA PR hat now ...)
Cheers, Nestor Your Friendly Neighborhood SysOp.
On 7/29/11 8:57 AM, ech-at-uvic.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Nestor } I haven't seen any comments about the on-line version. } } It is excellent. They did a really good job. } http://content.yudu.com/A1sxh8/MAMv17s1/resources/index.htm?referrerUrl=http%3A%2F%2Fjournals.cambridge.org%2Faction%2FdisplayJournal%3Fjid%3DMAM } } The bookmarks and the search engine work very well. } } Do you know what program they use to do this? } Elaine } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } John } } } } I have contacted Cambridge about this a few days ago } } as I discovered the same issues with the Flash version of EXPO. } } } } Cambridge has promised a full PDF downloadable version on-line } } in a few days. Hopefully it will be there by this coming Monday (7/1). } } } } Nestor } } Your Friendly Neighborhood SysOp } } & } } President - Microscopy Society of America } } } } } } } } } } } } -------- Original Message -------- } } Subject: [Microscopy] M and M 2011 Program } } Date: Fri, 29 Jul 2011 01:41:10 -0500 } } X-from: John.Mardinly-at-asu.edu } } Reply-To: John.Mardinly-at-asu.edu } } To: Nestor_Zaluzec-at-microscopy.com } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Has anybody tried to download a copy of the program from the Cambridge } } Journals web site? It displays on line as a Flash publication. There is } } an off-line version that can be downloaded, but it does not work on an } } ipad. There is a help menu that says any page range can be printed, but } } when I try to print it, it only allows 15 pages at a time to be printed, } } which is a pain because there are over 200 pages, and Flash crashes } } every time anyway when i try to print to PDF. What I really need it is } } PDF so I can load it onto my iPad and/or iPhone. Does anyone know how do } } do this? Is Cambridge going to post a more useful version? I am not } } going to put it on my laptop because my laptop weighs more than the } } printed version. Can we save some trees here? } } } } John Mardinly } } ASU } } } }
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America E.P. Wigner Fellow - Oak Ridge National Laboratory Senior Fellow of the Computational Institute - University of Chicago President Microscopy Society of America
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 22, 27 -- From zaluzec-at-aaem.amc.anl.gov Sat Jul 30 08:37:13 2011 22, 27 -- Received: from znl.com ([206.69.208.20]) 22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p6UDbDdf032086 22, 27 -- for {microscopy-at-microscopy.com} ; Sat, 30 Jul 2011 08:37:13 -0500 22, 27 -- Received: from localhost (localhost [127.0.0.1]) 22, 27 -- by znl.com (Postfix) with ESMTP id F33413E839D; 22, 27 -- Sat, 30 Jul 2011 08:37:12 -0500 (CDT) 22, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 22, 27 -- Received: from znl.com ([127.0.0.1]) 22, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 22, 27 -- with ESMTP id eJ8Ua7+cnCyl; Sat, 30 Jul 2011 08:37:12 -0500 (CDT) 22, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 22, 27 -- by znl.com (Postfix) with ESMTPA id 161E73E8392; 22, 27 -- Sat, 30 Jul 2011 08:37:12 -0500 (CDT) 22, 27 -- Message-ID: {4E340907.2000105-at-aaem.amc.anl.gov} 22, 27 -- Date: Sat, 30 Jul 2011 08:37:11 -0500 22, 27 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 22, 27 -- Organization: ANL 22, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 22, 27 -- MIME-Version: 1.0 22, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} , 22, 27 -- Aaron B Johnson {abjohnson-at-cambridge.org} 22, 27 -- Subject: Re: M and M 2011 Program online Version 22, 27 -- References: {201107291357.p6TDvslJ002007-at-ns.microscopy.com} 22, 27 -- In-Reply-To: {201107291357.p6TDvslJ002007-at-ns.microscopy.com} 22, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 22, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
what is the current status with deconvolution of AFM images please? Your suggestions and links on software, protocols and other resources would be highly appreciated.
With kind regards, Dmitry
==============================Original Headers============================== 4, 18 -- From d.sokolov-at-massey.ac.nz Mon Aug 1 00:42:41 2011 4, 18 -- Received: from mu-mail1.massey.ac.nz (mu-mail1.massey.ac.nz [130.123.129.15]) 4, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p715geUm032749 4, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 00:42:41 -0500 4, 18 -- Received: from TUR-MM2.massey.ac.nz (tur-mm2.massey.ac.nz [130.123.128.139]) 4, 18 -- by mu-mail1.massey.ac.nz (Postfix) with ESMTP id 2691A125ECE 4, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 17:42:39 +1200 (NZST) 4, 18 -- Received: from [127.0.0.1] (Not Verified[10.100.189.3]) by TUR-MM2.massey.ac.nz with MailMarshal 4, 18 -- id {B4e363cce0001} ; Mon, 01 Aug 2011 17:42:38 +1200 4, 18 -- Message-ID: {4E363CD1.6060808-at-massey.ac.nz} 4, 18 -- Date: Mon, 01 Aug 2011 17:42:41 +1200 4, 18 -- From: Dmitry Sokolov {d.sokolov-at-massey.ac.nz} 4, 18 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 4, 18 -- MIME-Version: 1.0 4, 18 -- To: Microscopy-at-microscopy.com 4, 18 -- Subject: AFM Deconvolution 4, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear Listers, our SEM Jeol JSM-5600LV is experiencing some problems in the filament heating current (thermoionic emission, W hairpin filament). At first the current was very unstable, fluctuating between about 15 and 150 µA. Now it has more or less stabilised, but at values definitely too high. I can try to clean the gun components, at least, but I've never opened an SEM gun. In particular, I wonder if there's some isolating gas like SF6 in TEM guns. Does anyone have advices for me? Thanks a lot
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 7, 19 -- From dcristofori-at-unive.it Mon Aug 1 05:07:11 2011 7, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71A7APr021489 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 05:07:11 -0500 7, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 7, 19 -- (authenticated bits=0) 7, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p71A75Jd006663 7, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 12:07:09 +0200 7, 19 -- Message-ID: {4E367D21.9080009-at-unive.it} 7, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 7, 19 -- Date: Mon, 01 Aug 2011 12:17:05 +0200 7, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 7, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 7, 19 -- MIME-Version: 1.0 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- Subject: SEM - filament heating current 7, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 7, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Opening the gun is easy, and there should be full directions in the SEM manual. Your symptoms sound like most like either the filament has physically drifted and needs to be (manually) recentered, or it has grown a whisker that is approaching the Wehnelt. If you are very careful, the whisker can be knocked off without disturbing the filament. Just make sure the now loose whisker is not rattling around in the Wehnelt, then reinstall the Wehnelt and you should be OK. But, expect to have to replace the filament if it is a whisker.
Phil
} Dear Listers, } our SEM Jeol JSM-5600LV is experiencing some problems in the filament } heating current (thermoionic emission, W hairpin filament). At first the } current was very unstable, fluctuating between about 15 and 150 µA. Now } it has more or less stabilised, but at values definitely too high. I can } try to clean the gun components, at least, but I've never opened an SEM } gun. In particular, I wonder if there's some isolating gas like SF6 in } TEM guns. } Does anyone have advices for me? } Thanks a lot } } Davide } } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Universita' Ca' Foscari Venezia } Dipartimento di Sc. Molecolari e Nanosistemi } Lab. di Scienza e Tecnologia dei Materiali } Via Torino, 155b } I-30172 Mestre (VE) } Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 29 -- From oshel1pe-at-cmich.edu Mon Aug 1 07:26:02 2011 5, 29 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71CQ2ss006762 5, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 07:26:02 -0500 5, 29 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 5, 29 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p71CQ1ot030387; 5, 29 -- Mon, 1 Aug 2011 08:26:02 -0400 5, 29 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 29 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.289.1; Mon, 1 Aug 5, 29 -- 2011 08:26:01 -0400 5, 29 -- MIME-Version: 1.0 5, 29 -- Message-ID: {a06240802ca5c4ae5166c-at-[141.209.160.249]} 5, 29 -- In-Reply-To: {201108011010.p71AAxJg026569-at-ns.microscopy.com} 5, 29 -- References: {201108011010.p71AAxJg026569-at-ns.microscopy.com} 5, 29 -- Date: Mon, 1 Aug 2011 08:25:59 -0400 5, 29 -- To: {Microscopy-at-microscopy.com} 5, 29 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 29 -- Subject: Re: [Microscopy] SEM - filament heating current 5, 29 -- CC: {dcristofori-at-unive.it} 5, 29 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 5, 29 -- X-Originating-IP: [141.209.160.249] 5, 29 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 29 -- X-Spam-Score: -0.50 () [Hold at 6.00] MIME_QP_LONG_LINE,_L_LLEXCH,Bayes(0.0001:-0.5) 5, 29 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 29 -- X-CanItPRO-Stream: default 5, 29 -- X-Canit-Stats-ID: 02Ff0q18w - ea5f64b5b571 - 20110801 5, 29 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p71CQ2ss006762 ==============================End of - Headers==============================
I am planning to retire from my present position in December, 2011. I will be staying local and will actively continue microscopy but from a different direction.
This is a great position for anyone interested in a challenging, multi-faceted job. Although the facility name is Life Science Microscopy, the position involves working with researchers form throughout the university. You never know what will come in the door, often from Engineering or the more physical sciences (Chemistry, Earth and Atmospheric Sciences, etc) as well as from the Colleges of Agriculture, Vet Medicine, etc. Lots of variety and great administrative support.
Along with the job comes a very pleasant location with strong academic, retail and manufacturing economy, good schools and affordable housing. We are only 1hr from Indianapolis and 2hrs from Chicago....easy day trips to both.
I will be at M&M so if you have any interest at all and would like to talk to me there than please contact me...or just contact me in any case and I can give you more information.
The formal position is not yet up but will be soon. It will be posted on the MSA jobs listing as well as through the Purdue University HR site as soon as it is finalized. Following is the tentative job description that is awaiting final HR approval before posting:
Debby
Director, Life Science Microscopy Facility Purdue University is seeking an individual with broad training in microscopy to direct a multiuser and service core facility. The individual will be responsible for the operation of the Life Science Microscopy Facility (LSMF) (http://www.ag.purdue.edu/facilities/microscopy). The applicant must be knowledgeable in theory and operation of transmission and scanning electron microscopes, light microscopes, and all related equipment. Although both non-biological and biological samples are routinely imaged, the individual must have a strong background in biological sample preparation. The individual is also responsible for routine maintenance of facilities, budgetary decisions, and supervision of support staff in addition to working directly with researchers on a daily basis. Teaching commitments will include major roles in microscopy theory/laboratory courses as well as one-on-one teaching of facility users. The individual must demonstrate a desire to remain current with technology and assist a faculty advisory committee in obtaining state-of-the-art equipment. Scholarly pursuit as a co-investigator with faculty on research projects is encouraged. The position requires an individual with strong inter-personal and multi-tasking skills. Good communication skills (oral and written) are essential.
The mission of the LSMF is to provide a broad range of Purdue researchers access to state-of-the-art microscopy instrumentation and to provide service, consultation and training to assist scientists in obtaining their research objectives. Technology in the LSMF provides capabilities for light, transmission, and scanning electron microscopy, and computer-based image analysis. Equipment is available for cryoSEM imaging, high pressure freezing and freeze substitution, ultramicrotomy, critical point drying, vacuum evaporation, sputter coating, microwave sample preparation, and other support instrumentation. Staff in the LSMF provides expertise in a wide range of specialized preparation techniques including immunocytochemistry and HPF-freeze substitution. Equipment in the facility includes FEI/Philips CM-100 and FEI/Philips CM-10 bio-twin transmission electron microscopes, FEI NOVA nanoSEM FESEM, FEI Quanta 3D FEG Dual-beam, and JEOL JSM-840 scanning electron microscopes.
Candidates should have an M.S. or Ph.D. degree and a minimum of 5 years post-graduate experience in a life-science microscopy field. Experience directing a multiuser facility is highly preferred.
Interested applicants should apply for the position on-line through the Purdue University Human Resources website ( position requisition ID number not yet assigned)
-- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/ Hi All,
==============================Original Headers============================== 18, 28 -- From dsherman-at-purdue.edu Mon Aug 1 07:50:22 2011 18, 28 -- Received: from mailhub131.itcs.purdue.edu (mailhub131.itcs.purdue.edu [128.210.5.131]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71CoM3g023349 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 07:50:22 -0500 18, 28 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 18, 28 -- by mailhub131.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p71CoM4A020567 18, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 08:50:22 -0400 18, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.217]) by WPPEXHUB04H.purdue.lcl 18, 28 -- ([::1]) with mapi; Mon, 1 Aug 2011 08:50:22 -0400 18, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 18, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 18, 28 -- Date: Mon, 1 Aug 2011 08:50:20 -0400 18, 28 -- Subject: Facility Director Position available 18, 28 -- Thread-Topic: Facility Director Position available 18, 28 -- Thread-Index: AcxQSZIM7oBmZyvIHECsaJGivbDrWA== 18, 28 -- Message-ID: {CA5C194C.11E1D%dsherman-at-purdue.edu} 18, 28 -- Accept-Language: en-US 18, 28 -- Content-Language: en-US 18, 28 -- X-MS-Has-Attach: 18, 28 -- X-MS-TNEF-Correlator: 18, 28 -- user-agent: Microsoft-Entourage/13.10.0.110428 18, 28 -- acceptlanguage: en-US 18, 28 -- Content-Type: text/plain; charset="us-ascii" 18, 28 -- MIME-Version: 1.0 18, 28 -- X-PMX-Version: 5.5.9.388399 18, 28 -- X-PerlMx-Virus-Scanned: Yes 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p71CoM3g023349 ==============================End of - Headers==============================
Dear All, Thank you for your quick replies and suggestions. I forgot to say I checked the filament and no wiskers are present. Indeed, the problem is present also with a couple of brand new filaments, from different boxes (but not sure they're from different batches). Also the filament position looks the right one, and actually the hardware doesn't allow for such big drifts. As for opening the gun, I mean "dismantle", because the idea was to check for some problem on electrical contacts for the heating current and high tension. Just an attempt, because we had a similar problem with TEM time ago. So, is it safe for the gun to dismantle it without knowing how the instrument is built? May there be SF6 or any other insulating gas? Thanks again
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 5, 19 -- From dcristofori-at-unive.it Mon Aug 1 08:07:59 2011 5, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71D7w19007362 5, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 08:07:58 -0500 5, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 5, 19 -- (authenticated bits=0) 5, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p71D7vUK007251 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 15:07:57 +0200 5, 19 -- Message-ID: {4E36A786.2050909-at-unive.it} 5, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 5, 19 -- Date: Mon, 01 Aug 2011 15:17:58 +0200 5, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 5, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: SEM - filament heating current: addenda 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Location : East Coast, USA (preferably Upstate, NY region - Albany, East Fishkill)
Brief Description & Qualifications : Newly created role to support increased demand on East Coast for growing and leading edge SEM/TEM sample preparation equipment division of global semiconductor manufacturer. Seeking a talented, practical technologist/engineer possessing 3 years+ "best practice" industry exposure in technical support and field engineering within advanced microscopy (TEM, SEM) sample prep environment. This professional is going to oversee primarily East Coast (United States) installation, qualification and acceptance of advanced sample prep inspection systems at customer sites. More comprehensive description available upon request. =20
BS Engineering/Physics required. Technical Masters desirable. Ability and desire to travel 40%. Must be comfortable working independently at customer sites.
Please reply directly to Jeff Dollar Thank you!
==============================Original Headers============================== 6, 20 -- From Jeffd-at-capstone-inc.com Mon Aug 1 08:16:25 2011 6, 20 -- Received: from capstone-inc.com (mail.capstone-inc.com [24.97.8.122]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71DGOax023663 6, 20 -- for {Microscopy-at-Microscopy.Com} ; Mon, 1 Aug 2011 08:16:25 -0500 6, 20 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 6, 20 -- Content-class: urn:content-classes:message 6, 20 -- MIME-Version: 1.0 6, 20 -- Content-Type: text/plain; 6, 20 -- charset="us-ascii" 6, 20 -- Subject: Field Application Engineer Opening - East Coast United States 6, 20 -- Date: Mon, 1 Aug 2011 09:16:24 -0400 6, 20 -- Message-ID: {D7593C9967520F4F85F0BB59B670FED9AA1975-at-mail.capstone-inc.com} 6, 20 -- X-MS-Has-Attach: 6, 20 -- X-MS-TNEF-Correlator: 6, 20 -- Thread-Topic: Field Application Engineer Opening - East Coast United States 6, 20 -- Thread-Index: AcxQTT0zMuIzyIx8QS+Kt7k1ksy0Iw== 6, 20 -- From: "Jeff Dollar" {Jeffd-at-capstone-inc.com} 6, 20 -- To: {Microscopy-at-Microscopy.Com} 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p71DGOax023663 ==============================End of - Headers==============================
Davide, I'm assuming the gun is of similar construction to the 5200, 5300, 5400. There is no gas. There are pieces of red silicone rubber and a machined nylon core. This can all be disassembled, cleaned, inspected carefully for arc tracks and reassembled with a small amount of silicone grease on the silicone rubber parts.
It is a smaller version of the 840/6400 gun.
I have had to get a new nylon piece machined for a 6400 that had arced through due to mostly being operated at 40kV. I also had a 5200 with a bad cable which I tried repairing/replacing several times but had to finally give up and by the cable/gun assembly from JEOL. They would only sell the complete assembly.
Before you go through all of that, is the gun vacuum good enough? Having the emission current bounce all around could possibly be arcing in the gun due to poor vacuum. The arcing could also have damaged components in the HT tank. Check the bias circuit carefully.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: Monday, August 01, 2011 9:10 AM To: kenconverse-at-qualityimages.biz
Dear All, Thank you for your quick replies and suggestions. I forgot to say I checked the filament and no wiskers are present. Indeed, the problem is present also with a couple of brand new filaments, from different boxes (but not sure they're from different batches). Also the filament position looks the right one, and actually the hardware doesn't allow for such big drifts. As for opening the gun, I mean "dismantle", because the idea was to check for some problem on electrical contacts for the heating current and high tension. Just an attempt, because we had a similar problem with TEM time ago. So, is it safe for the gun to dismantle it without knowing how the instrument is built? May there be SF6 or any other insulating gas? Thanks again
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 5, 19 -- From dcristofori-at-unive.it Mon Aug 1 08:07:59 2011 5, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 5, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71D7w19007362 5, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 08:07:58 -0500 5, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 5, 19 -- (authenticated bits=0) 5, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p71D7vUK007251 5, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 5, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 15:07:57 +0200 5, 19 -- Message-ID: {4E36A786.2050909-at-unive.it} 5, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 5, 19 -- Date: Mon, 01 Aug 2011 15:17:58 +0200 5, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 5, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 5, 19 -- MIME-Version: 1.0 5, 19 -- To: Microscopy-at-microscopy.com 5, 19 -- Subject: SEM - filament heating current: addenda 5, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 5, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From kenconverse-at-qualityimages.biz Mon Aug 1 08:46:12 2011 19, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71DkBG3007767 19, 28 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 08:46:11 -0500 19, 28 -- X-Authority-Analysis: v=1.1 cv=8mDY8c80ZOa76EOwICuS+E2YRQjxDgO9xqUnRMONc7w= c=1 sm=0 a=4gAfbMTMWB8A:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Zx37jsudAAAA:8 a=uqGNuOHP2nwR3MdEBTQA:9 a=Rfp6n-5o_fE21HqDgR8A:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=PhGD0Z7zLlKKZZSM:21 a=HBo4uGb0iSJiYhYv:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 19, 28 -- X-Cloudmark-Score: 0 19, 28 -- X-Originating-IP: 72.224.149.57 19, 28 -- Received: from [72.224.149.57] ([72.224.149.57:4008] helo=Ken) 19, 28 -- by cdptpa-oedge03.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 19, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 19, 28 -- id D9/06-00666-32EA63E4; Mon, 01 Aug 2011 13:46:11 +0000 19, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 19, 28 -- To: {dcristofori-at-unive.it} , "MSA Listserver" {microscopy-at-microscopy.com} 19, 28 -- Subject: RE: [Microscopy] SEM - filament heating current: addenda 19, 28 -- Date: Mon, 1 Aug 2011 09:45:53 -0400 19, 28 -- Message-ID: {6A297E250B5A452BA2038813EC87C705-at-Ken} 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="us-ascii" 19, 28 -- X-Priority: 3 (Normal) 19, 28 -- X-MSMail-Priority: Normal 19, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 19, 28 -- Importance: Normal 19, 28 -- Thread-Index: AcxQTEREi8enRMLdTlSYGxD8f8LczgAAuKrg 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 19, 28 -- In-Reply-To: {201108011309.p71D9b99010792-at-ns.microscopy.com} 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p71DkBG3007767 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both abjohnson-at-cambridge.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: abjohnson-at-cambridge.org Name: Aaron Bernard Johnson
Organization: Cambridge Journals
Title-Subject: [Filtered] M&M 2011 EXPO: single PDF to download (Apple friendly!)
Message: Dear Microscopy Community,
I write to you as the Cambridge Journals publisher for the Microscopy & Microanalysis 2011 EXPO. As several of you have pointed, out the magazine page-turning view does not work on an iPad because of Adobe Flash compatibility with Apple. However, there are now 2 options:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dzhou-at-tcd.ie as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dzhou-at-tcd.ie Name: Dan Zhou
Organization: CRANN, TCD, Ireland
Title-Subject: [Filtered] 3D tomography in SEM
Message: Hi, all. May I know is there any free or open source software specifically suitable for 3D tomography in SEM?
I know some very good in TEM tomography, but not sure if they suit SEM.
First I agree with you it seems that the gun may be dirty or the vacuum system is inadequate. The fact that the system improved would be the vacuum improving but you still need to clean the gun area. I would still worry about electrical connections and possibly the high voltage cable but first thing first make sure that the gun chamber is clean.
The electron gun in an SEM is a very simple system. The cathode assembly is fixed to a ceramic insulator and the anode screws (or simply fits inside) the top of the first condenser lens and the gun alignment coil system.
There are a vast array of cleaning media used by laboratories to clean the cathode assemblies of electron microscopes. With many of these the biggest failing is the difficulty of removing completely the cleaning media leading to excessive contamination within the system. This problem is further complicated by the human hazards associated with some of the solvents being used. In some countries acetone and ether are not permitted in the laboratory.
In the best technique the cathode assembly is placed, aperture face upwards, in a beaker of stock ammonia solution diluted 3 parts ammonia to one part water. The stock solution may be 30 to 40% ammonia. After 15 minutes in the ultrasonic cleaner the beaker is placed under running water and thoroughly flushed through. Care should be taken to ensure that none of the clamping or alignment screws had fallen out of the cathode assembly and could be flushed away! The cathode is then washed with alcohol before being dried with a hair drier. A new filament should be fitted and centred. The assembly should be checked for cleanliness by observing with a 10X lens prior to re installation in the microscope. Total time for this procedure should be less than 25 minutes from filament break to pump down.
Great care should be taken not to allow the ammonia solution to make contact with the skin or eyes of the operator. When flushing the solution through with water its flow should be set so as not to splash the solution over the operator prior to placing the beaker under the flow.
The anode should be cleaned in a similar fashion.
If the metal walls of the gun chamber are coloured they too should be cleaned using 1 part ammonia solution with 10 parts of water. It would be ideal to remove the gun chamber but with care it may be cleaned in situ. Once clean it should be heated with a hair dryer to out gas. Cover the anode area with aluminium foil during cleaning and use damped fluff free but absorbent tissue to apply the solution.
Check all of the "O" rings that are disturbed and wash them, like washing your hands, in hot soapy water. Clean the "O" ring seats and flat faces with a solvent.
I hope that this helps but if you need more please come back to me.
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 01 August 2011 11:08 To: protrain-at-emcourses.com
Dear Listers, our SEM Jeol JSM-5600LV is experiencing some problems in the filament heating current (thermoionic emission, W hairpin filament). At first the current was very unstable, fluctuating between about 15 and 150 µA. Now it has more or less stabilised, but at values definitely too high. I can try to clean the gun components, at least, but I've never opened an SEM gun. In particular, I wonder if there's some isolating gas like SF6 in TEM guns. Does anyone have advices for me? Thanks a lot
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 7, 19 -- From dcristofori-at-unive.it Mon Aug 1 05:07:11 2011 7, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p71A7APr021489 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 05:07:11 -0500 7, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 7, 19 -- (authenticated bits=0) 7, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p71A75Jd006663 7, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 7, 19 -- for {microscopy-at-microscopy.com} ; Mon, 1 Aug 2011 12:07:09 +0200 7, 19 -- Message-ID: {4E367D21.9080009-at-unive.it} 7, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 7, 19 -- Date: Mon, 01 Aug 2011 12:17:05 +0200 7, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 7, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 7, 19 -- MIME-Version: 1.0 7, 19 -- To: microscopy-at-microscopy.com 7, 19 -- Subject: SEM - filament heating current 7, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 7, 19 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 27, 24 -- From protrain-at-emcourses.com Tue Aug 2 13:04:05 2011 27, 24 -- Received: from mail.esentra.net (mail.esentra.net [94.228.36.208]) 27, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p72I44CP018322 27, 24 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 13:04:05 -0500 27, 24 -- Received: from mail.clientmail.net (mail.clientmail.net [127.0.0.1]) by mail.esentra.net with MailMarshal 27, 24 -- id {B4e383c000000} ; Tue, 02 Aug 2011 19:03:44 +0100 27, 24 -- Reply-To: {protrain-at-emcourses.com} 27, 24 -- From: {protrain-at-emcourses.com} 27, 24 -- To: {dcristofori-at-unive.it} 27, 24 -- Cc: "Microscopical Soc of America" {microscopy-at-microscopy.com} 27, 24 -- References: {201108011008.p71A8I7s022688-at-ns.microscopy.com} 27, 24 -- In-Reply-To: {201108011008.p71A8I7s022688-at-ns.microscopy.com} 27, 24 -- Subject: RE: [Microscopy] SEM - filament heating current 27, 24 -- Date: Tue, 2 Aug 2011 19:03:56 +0100 27, 24 -- Organization: protrain-at-emcourses.com 27, 24 -- Message-ID: {00bf01cc513e$8c48df70$a4da9e50$-at-com} 27, 24 -- MIME-Version: 1.0 27, 24 -- Content-Type: text/plain; 27, 24 -- charset="iso-8859-1" 27, 24 -- X-Mailer: Microsoft Office Outlook 12.0 27, 24 -- Thread-Index: AcxRLKqha65Aqz7cRv6ISkNkFgJKbQADcAdA 27, 24 -- Content-Language: en-gb 27, 24 -- Content-Transfer-Encoding: 8bit 27, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p72I44CP018322 ==============================End of - Headers==============================
Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity:
The company currently has an opening for a Technical Service Engineer based in Berlin Germany. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary.
Salary: Commensurate with experience
Other: Company car
Primary Responsibilities:
The Technical Service Engineer will visit customers for preventative maintenance, repairs, fault analysis, phone support and upgrades in order to achieve optimum customer care and ensure a professional repair in due time with effective and efficient working practice. The Technical Service Engineer will also deliver, promote and sell high quality technical service within the area of responsibility with the objective of achieving customer satisfaction while obtaining individual or team revenue and targets.
Additional Responsibilities: - Work closely with the Service Team to ensure all customers in territory are provided a professional After Sales service - Work closely with Sales Reps within the respective Segment in order to support activities such as Installations and Demo/Exhibitions - Exchange valuable information resulting from customer contacts to increase customer satisfaction and sales - Work closely with Customer Care in order to ensure smooth and fast processes and information flow - Deliver accurate reporting, handling and timely submission of Mobile Service reports, Expenses, Spares Stock management and other Service documentation
Education and Experience Required:
- Degree in Engineering; Familiarity with standard IT tools; Experienced Technical Engineer with qualifications in IT (Connectivity and Networking) - Ability to demonstrate understanding of basic Optical principles and demonstrate sound Electronic and Instrumentation engineering skills - Ability to plan own work and work unsupervised - Fluency in German, English capable to communicate, and strong communication skills
If you meet and/or exceed the experience criteria, please submit your resume by emailing Christy Edwards (Sr. e-Recruitment Consultant with Personify). We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
==============================Original Headers============================== 16, 19 -- From ce-at-personifysearch.com Tue Aug 2 15:12:36 2011 16, 19 -- Received: from na3sys010aob108.obsmtp.com (na3sys010aob108.obsmtp.com [74.125.245.83]) 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p72KCZgF023292 16, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 15:12:35 -0500 16, 19 -- Received: from mail-pz0-f43.google.com ([209.85.210.43]) (using TLSv1) by na3sys010aob108.postini.com ([74.125.244.12]) with SMTP 16, 19 -- ID DSNKTjhaMt+6iFa3Uy6bLha9SeelLURuP8/F-at-postini.com; Tue, 02 Aug 2011 13:12:35 PDT 16, 19 -- Received: by mail-pz0-f43.google.com with SMTP id 1so160669pzk.2 16, 19 -- for {microscopy-at-microscopy.com} ; Tue, 02 Aug 2011 13:12:33 -0700 (PDT) 16, 19 -- Received: by 10.68.24.103 with SMTP id t7mr3869926pbf.419.1312315952974; Tue, 16, 19 -- 02 Aug 2011 13:12:32 -0700 (PDT) 16, 19 -- From: Christy Edwards {ce-at-personifysearch.com} 16, 19 -- MIME-Version: 1.0 16, 19 -- X-Mailer: Microsoft Office Outlook 12.0 16, 19 -- Thread-Index: AcxRUIK9nz5x+sOgSXqCqFseZP5yzA== 16, 19 -- Date: Tue, 2 Aug 2011 16:12:31 -0400 16, 19 -- Message-ID: {645879fe96f33327c92e399193305e18-at-mail.gmail.com} 16, 19 -- Subject: Technical Service Engineer Opportunity in Berlin Germany 16, 19 -- To: microscopy-at-microscopy.com 16, 19 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity:
The company currently has an opening for an R&D Manager for the business segment, Digital Pathology. Ideal base for this position is Germany. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary.
Salary: Commensurate with experience
Primary Responsibilities:
The R&D Manager is responsible for all Research and Development issues regarding Virtual Microscopes/Digital Pathology and will manage R&D teams in Dublin, Ireland and Wetzlar, Germany (approx. 20 employees working in development, testing, system engineering and project management).
Additional Responsibilities: - Build Research and Development (R&D) Team; Alignment of existing teams in Dublin, Ireland and Wetzlar Germany; Lead, develop and rate R&D staff - Insourcing software development and testing team for Slide Scanner; Implement system engineer - Support in preparation and implementation of Digital Pathology strategy and transfer/lead over into roadmap portfolio - Definition and implementation of whole Slide Scanner strategy and Software-application strategy (together with Production Management) - Install medium-term capacity planning for R&D Digital Pathology and sourcing strategy for all critical components - Agree on portfolio and technology topics with Program Management and Management R&D Clinical Imaging
Education and Experience Required:
- University degree in Engineering or Sciences - Serveral years of experience in R&D (preferalbly hard- and software) including some years in teamleader or project leader role - Experience in Medical Device Industry (regulated environment) - Broad knowledge of modern hard- and software development techniques - Experience in cross-platform systems integration - Good leadership and project managment skills - Knowledge in optics and mechanics is preferable
If you meet and/or exceed the experience criteria, please submit your resume by emailing Christy Edwards (Sr. e-Recruitment Consultant with Personify) at ce-at-personifysearch.com. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
==============================Original Headers============================== 16, 19 -- From ce-at-personifysearch.com Tue Aug 2 15:19:19 2011 16, 19 -- Received: from na3sys010aob103.obsmtp.com (na3sys010aob103.obsmtp.com [74.125.245.73]) 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p72KJJWh004846 16, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 15:19:19 -0500 16, 19 -- Received: from mail-pz0-f42.google.com ([209.85.210.42]) (using TLSv1) by na3sys010aob103.postini.com ([74.125.244.12]) with SMTP 16, 19 -- ID DSNKTjhbxf0TH/3g6H33RLCe5lhF67c58yN4-at-postini.com; Tue, 02 Aug 2011 13:19:18 PDT 16, 19 -- Received: by mail-pz0-f42.google.com with SMTP id 37so203619pzk.15 16, 19 -- for {microscopy-at-microscopy.com} ; Tue, 02 Aug 2011 13:19:17 -0700 (PDT) 16, 19 -- Received: by 10.68.40.232 with SMTP id a8mr11094873pbl.84.1312316357526; Tue, 16, 19 -- 02 Aug 2011 13:19:17 -0700 (PDT) 16, 19 -- From: Christy Edwards {ce-at-personifysearch.com} 16, 19 -- MIME-Version: 1.0 16, 19 -- X-Mailer: Microsoft Office Outlook 12.0 16, 19 -- Thread-Index: AcxRUXQnK9kQhGLeSLykj72mjUhOrw== 16, 19 -- Date: Tue, 2 Aug 2011 16:19:16 -0400 16, 19 -- Message-ID: {f68090b57ebf502ce139aa8aa4479c11-at-mail.gmail.com} 16, 19 -- Subject: R&D Manager - Digital Pathology Opportunity in Germany 16, 19 -- To: microscopy-at-microscopy.com 16, 19 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity:
The company currently has an opening for a Technical Service Engineer based in Berlin Germany. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary.
Salary: Commensurate with experience
Other: Company car
Primary Responsibilities:
The Technical Service Engineer will visit customers for preventative maintenance, repairs, fault analysis, phone support and upgrades in order to achieve optimum customer care and ensure a professional repair in due time with effective and efficient working practice. The Technical Service Engineer will also deliver, promote and sell high quality technical service within the area of responsibility with the objective of achieving customer satisfaction while obtaining individual or team revenue and targets.
Additional Responsibilities: - Work closely with the Service Team to ensure all customers in territory are provided a professional After Sales service - Work closely with Sales Reps within the respective Segment in order to support activities such as Installations and Demo/Exhibitions - Exchange valuable information resulting from customer contacts to increase customer satisfaction and sales - Work closely with Customer Care in order to ensure smooth and fast processes and information flow - Deliver accurate reporting, handling and timely submission of Mobile Service reports, Expenses, Spares Stock management and other Service documentation
Education and Experience Required:
- Degree in Engineering; Familiarity with standard IT tools; Experienced Technical Engineer with qualifications in IT (Connectivity and Networking) - Ability to demonstrate understanding of basic Optical principles and demonstrate sound Electronic and Instrumentation engineering skills - Ability to plan own work and work unsupervised - Fluency in German, English capable to communicate, and strong communication skills
If you meet and/or exceed the experience criteria, please submit your resume by emailing Christy Edwards (Sr. e-Recruitment Consultant with Personify) at ce-at-personifysearch.com. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
==============================Original Headers============================== 17, 19 -- From ce-at-personifysearch.com Tue Aug 2 15:22:55 2011 17, 19 -- Received: from na3sys010aob104.obsmtp.com (na3sys010aob104.obsmtp.com [74.125.245.75]) 17, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p72KMt6n014869 17, 19 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 15:22:55 -0500 17, 19 -- Received: from mail-pz0-f41.google.com ([209.85.210.41]) (using TLSv1) by na3sys010aob104.postini.com ([74.125.244.12]) with SMTP 17, 19 -- ID DSNKTjhcnUERLEOeY6+UzmNihqsEzlMVSYC2-at-postini.com; Tue, 02 Aug 2011 13:22:54 PDT 17, 19 -- Received: by mail-pz0-f41.google.com with SMTP id 4so242784pzk.0 17, 19 -- for {microscopy-at-microscopy.com} ; Tue, 02 Aug 2011 13:22:53 -0700 (PDT) 17, 19 -- Received: by 10.68.24.103 with SMTP id t7mr3886363pbf.419.1312316573640; Tue, 17, 19 -- 02 Aug 2011 13:22:53 -0700 (PDT) 17, 19 -- From: Christy Edwards {ce-at-personifysearch.com} 17, 19 -- MIME-Version: 1.0 17, 19 -- X-Mailer: Microsoft Office Outlook 12.0 17, 19 -- Thread-Index: AcxRUfT2UeI/sYTtTmuGaRha+wglcg== 17, 19 -- Date: Tue, 2 Aug 2011 16:22:53 -0400 17, 19 -- Message-ID: {522442546f325d2ef1b960b4a1e4f613-at-mail.gmail.com} 17, 19 -- Subject: Technical Service Engineer Opportunity in Berlin Germany 17, 19 -- To: microscopy-at-microscopy.com 17, 19 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
I saw the spam posting from AOL. This subscription has been disabled so no additional posting should be forthcoming. Investigating it appears that the AOL Email address has been hijacked/broken into. I have informed the user of the problem with their accounts.
Cheers,
Nestor Your Friendly Neighborhood SysOp
==============================Original Headers============================== 4, 25 -- From zaluzec-at-aaem.amc.anl.gov Tue Aug 2 19:57:59 2011 4, 25 -- Received: from znl.com ([206.69.208.20]) 4, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p730vx8K010502 4, 25 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 19:57:59 -0500 4, 25 -- Received: from localhost (localhost [127.0.0.1]) 4, 25 -- by znl.com (Postfix) with ESMTP id 32F9E3EAD8F 4, 25 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 19:57:59 -0500 (CDT) 4, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 4, 25 -- Received: from znl.com ([127.0.0.1]) 4, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 4, 25 -- with ESMTP id FskaB29mSCls for {microscopy-at-microscopy.com} ; 4, 25 -- Tue, 2 Aug 2011 19:57:58 -0500 (CDT) 4, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 4, 25 -- by znl.com (Postfix) with ESMTPA id 684583EAD84 4, 25 -- for {microscopy-at-microscopy.com} ; Tue, 2 Aug 2011 19:57:58 -0500 (CDT) 4, 25 -- Message-ID: {4E389D16.7020506-at-aaem.amc.anl.gov} 4, 25 -- Date: Tue, 02 Aug 2011 19:57:58 -0500 4, 25 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 4, 25 -- Organization: ANL 4, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 4, 25 -- MIME-Version: 1.0 4, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 4, 25 -- Subject: Administrivia: Spam from AOL User 4, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Just one additional thought: did you insert the objective aperture? Perhaps on the 200kV (EDX?) you mainly work with the aperture out. It needs to be inserted otherwise there is a big chance that you blow up your section.
Regards Stephane
----- Original Message ---- X-from: "Marcela.Redigolo-at-mail.wvu.edu" {Marcela.Redigolo-at-mail.wvu.edu} To: nizets2-at-yahoo.com Sent: Thu, July 28, 2011 3:14:46 PM
Dear all:
Thanks for all the replies and many suggestions. We indeed never worked with the scope at 80KV and you are correct, Elaine. The alignment is way off. Still, I'll keep this idea in mind. It will be very helpful to have the scope
operating on both accelerating voltages. But before doing this, I'll try many other ideas that were suggested for this biosample in particular.
Thanks again to all for your replies. I really appreciate!
Kindest regards,
Marcela.
} } } 07/28/11 9:01 AM } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hello Steve and Fellow Listers,
Just to add what may be an obvious note about operating at a lower accelerating voltage. If the 2100 has never been aligned at anything other than 200kV, you may need to have a service engineer in to do initial alignments at lower voltages. When our JEOL JEM-3010 was installed, the engineers did alignments at 50kV intervals from 50 to 300kV and stored those values for future use. On the rare occasions when I use a lower accelerating voltage, I just have to do some minor touch-up to the alignment. Also, you'll want to check your magnification calibration at the lower accelerating voltage.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. ********************************************************************* -----Original Message----- X-from: protrain-at-emcourses.com [mailto:protrain-at-emcourses.com] Sent: Thursday, July 28, 2011 3:35 AM To: Elaine F. Schumacher
Dear Marcela
Your 200kV microscope will work very well down at 80kV and when you return to 200kV it will regain its absolute stability quite quickly.
I had a similar incident with a client who also worried about changing from their 200kV JEOL. I had the client take test pictures of the gold lattice from 200kV down to 80kV in 20kV steps; she was 100% successful!
Enjoy
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
Title-Subject: [Filtered] Biosample in TEM 80 KV and 200 KV
Message: Dear all:
We have one user that prepares his biological tissues in resin and then cut them for TEM studies. He has been using a 80KV TEM for that, but that TEM does't have digital camera and he wants to start using ours.
We have a JEOL 2100 TEM operating at 200KV. We can run the sample and do some imaging while playing a bit with the settings of the scope, but still in some cases the sample ends up burning. I'm not experienced with biological samples.
Is there a different sample preparation he should look for, or maybe change his resin or do cryo-cut, before coming to our scope, or is there a certain group of settings I should work with for this particular case? Thanks for any help.
there was a posting on 'tomo and SEM' on the listserver and I accidentally hit the delete button. Anyway: I know that Paul Walther in Ulm , Germany , did something in this direction, and showed it on a conference. You may contact him directly. - As far as I remember, he collected the data straight forward by tilting the specimen in the SEM (is every SEM able to do this ...? eucentric height??) and processed the data straight away in IMOD (Boulder; David Mastronarde and co!). -- Paul is the person to ask. kind regards - Reinhard
-- Prof. Dr. Reinhard Rachel Universitaet Regensburg Centre for EM / Anatomy Faculty for Biology&Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 4, 23 -- From reinhard.rachel-at-biologie.uni-regensburg.de Wed Aug 3 06:44:17 2011 4, 23 -- Received: from rrzmta5.uni-regensburg.de (rrzmta5.uni-regensburg.de [194.94.155.56]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p73BiG7x019519 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 06:44:17 -0500 4, 23 -- Received: from rrzmta5.uni-regensburg.de (localhost [127.0.0.1]) 4, 23 -- by localhost (Postfix) with SMTP id ED0A741C9 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 13:44:12 +0200 (CEST) 4, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 4, 23 -- by rrzmta5.uni-regensburg.de (Postfix) with ESMTP id DB738417B 4, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 13:44:12 +0200 (CEST) 4, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 4, 23 -- with Novell_GroupWise; Wed, 03 Aug 2011 13:44:13 +0200 4, 23 -- Message-Id: {4E3950AB0200005400029FE7-at-gwsmtp1.uni-regensburg.de} 4, 23 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 4, 23 -- Date: Wed, 03 Aug 2011 13:44:11 +0200 4, 23 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 4, 23 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 4, 23 -- Subject: tomo and SEM 4, 23 -- Mime-Version: 1.0 4, 23 -- Content-Type: text/plain; charset=US-ASCII 4, 23 -- Content-Disposition: inline 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p73BiG7x019519 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scott.wight-at-nist.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Abstracts are now being accepted for the Cathodoluminescence Topical Conference to be held at NIST in Gaithersburg, MD, USA on October 24-28, 2011. Please see the conference website for details: http://www.microbeamanalysis.org/topical-conferences/cl-2011
Dear Listers, thank you so much for your support! You gave me very useful and detailed information! I'd like to follow your advice to monitor the vacuum in the gun, but actually I don't know how to do it: there's no gauge reading in the software, so the gauge output is just a voltage signal which I'm not sure to be able to measure and to convert in pressure. And no aperture for an external vacuum gauge in the gun. Maybe all of this is getting too technical and not interesting for other Listers. If you feel this is the case, please let me know. If in trouble, I'll contact privately some of you. Thanks again
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 6, 19 -- From dcristofori-at-unive.it Wed Aug 3 08:40:30 2011 6, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p73DeSqq030364 6, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 08:40:29 -0500 6, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 6, 19 -- (authenticated bits=0) 6, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p73DeQXb013631 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 15:40:26 +0200 6, 19 -- Message-ID: {4E395229.60206-at-unive.it} 6, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 6, 19 -- Date: Wed, 03 Aug 2011 15:50:33 +0200 6, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 6, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 6, 19 -- MIME-Version: 1.0 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- Subject: RE: SEM - filament heating current 6, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There has been a change in instructors for the Sunday short course X14: 3D Electron Microscopy of Macromolecular Assemblies. We are glad to announce that the section on docking of X-ray structures will now be taught by Dr. Edward Morris from the Structural Biology group at the Institute of Cancer Research in London, UK.
Course description (on-site registration is possible):
X14: 3D Electron Microscopy of Macromolecular Assemblies Teresa Ruiz, Michael Radermacher, and Edward Morris
This short course will provide a comprehensive description of the methods used for 3D structure determination of macromolecular complexes from electron micrographs. First specimen preparation techniques for single particles (deep stain, vitreous ice), will be presented and the selection of imaging conditions including low-dose imaging. This will be followed by a detailed explanation of image processing techniques with special emphasis on the random conical reconstruction technique. In the last part structure interpretation and docking of X-ray structures to 3D EM densities will be demonstrated. The techniques described have application in both biological and materials science.
-- Michael Radermacher, Ph.D., Prof. University of Vermont Dept. Molecular Physiology and Biophysics HSRF 120 / 149 Beaumont Ave Burlington, VT 05405 USA
==============================Original Headers============================== 11, 24 -- From mraderma-at-uvm.edu Wed Aug 3 17:10:18 2011 11, 24 -- Received: from smtp1.uvm.edu (smtp1.uvm.edu [132.198.101.168]) 11, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p73MAHad029197 11, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 17:10:17 -0500 11, 24 -- Received: from uvm.edu (penguin4.uvm.edu [132.198.101.214]) 11, 24 -- by smtp1.uvm.edu (8.14.2/8.14.2) with ESMTP id p73MAFHJ030797 11, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 18:10:15 -0400 11, 24 -- Received: from egress.sbb.uvm.edu (egress.sbb.uvm.edu [132.198.90.90]) by 11, 24 -- webmail.uvm.edu (Horde MIME library) with HTTP; Wed, 3 Aug 2011 18:10:15 11, 24 -- -0400 11, 24 -- Message-ID: {20110803181015.gss47k7jbmokgg08-at-webmail.uvm.edu} 11, 24 -- Date: Wed, 3 Aug 2011 18:10:15 -0400 11, 24 -- From: Michael Radermacher {mraderma-at-uvm.edu} 11, 24 -- To: Microscopy-at-microscopy.com 11, 24 -- Subject: Instructor Change, Sunday short course X14 11, 24 -- MIME-Version: 1.0 11, 24 -- Content-Type: text/plain; 11, 24 -- charset=ISO-8859-1; 11, 24 -- DelSp="Yes"; 11, 24 -- format="flowed" 11, 24 -- Content-Disposition: inline 11, 24 -- User-Agent: Internet Messaging Program (IMP) H3 (4.1.4) 11, 24 -- Content-Transfer-Encoding: 8bit 11, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p73MAHad029197 ==============================End of - Headers==============================
A researcher at our institution is writing a grant proposal and would like to include two light microscopes in the proposal: (1) an inverted, phase microscope with objectives of 5, 10, 20 and 40X, (2) a stereomicroscope with bottom lighting and a magnification range of 5 to 40X.
She mostly would be looking at large cells (swimming algae) and trying to "pick" individual cells using a fine glass pipette. We have been doing this on my Olympus SZH10 but she now wants her own system. Price would be a consideration; therefore, a "price range" would be the best approach.
Thank you.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 5, 18 -- From bozzola-at-siu.edu Wed Aug 3 17:46:41 2011 5, 18 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p73MkfbI013304 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Aug 2011 17:46:41 -0500 5, 18 -- Received: by vxj15 with SMTP id 15so39923vxj.0 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 03 Aug 2011 15:46:38 -0700 (PDT) 5, 18 -- MIME-Version: 1.0 5, 18 -- Received: by 10.52.97.1 with SMTP id dw1mr93919vdb.307.1312411597923; Wed, 03 5, 18 -- Aug 2011 15:46:37 -0700 (PDT) 5, 18 -- Received: by 10.52.162.70 with HTTP; Wed, 3 Aug 2011 15:46:37 -0700 (PDT) 5, 18 -- Date: Wed, 3 Aug 2011 17:46:37 -0500 5, 18 -- Message-ID: {CAKeCJ9xzXtPWHA3LDnCG7NkecQjRjxDgecjLuVDprR-vCkqK3g-at-mail.gmail.com} 5, 18 -- Subject: LM: pricing quotes 5, 18 -- From: John Bozzola {bozzola-at-siu.edu} 5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 18 -- Content-Type: text/plain; charset=UTF-8 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p73MkfbI013304 ==============================End of - Headers==============================
Dear All, Registration is Now Open for the 2012 Advanced Microscopy and Digital Imaging Workshop at UC, Santa Barbara. January 17-20, 2012 Offered by the Neuroscience Research Institute (NRI) and Department of Molecular, Cellular, and Developmental Biology (MCDB).
For more details please see https://www.lifesci.ucsb.edu/mcdb/events/imaging_workshop/
Best Regards Mary
-- Mary Raven Microscopy Facility Director Neuroscience Research Institute& Molecular, Cellular& Developmental Biology Biology 2 Building, room 5173 The University of California Santa Barbara, CA 93106-5060
Hello All, I am attempting to remedy an issue with a Conductavac IV sputter coater by See Vac. Recently, it was observed that the ammeter needle was wavering considerably during the sputter coating process. The needle would be at 0 mA and with a slight twist of the Current Adjust Knob the needle would be up near 40mA(~ half way up ammeter). I assumed there might be a short somewhere in the system and after checking all the connections and making sure all junctions were snug no obvious problems were observed. Another sputter coating run was attempted but to no avail. However, the needle now moves with more fluidity. Control of the needle has been regained though the needle currently maxes out(100mA) where the needle would typically be at 30mA(proper amps for general sputter coating). Why the rapid increase in amps? I have also clean the anode and cathode hoping the removing any debris would help solve my issue. The vacuum pumping out the system is working fine and easily pumps below 100mTorr. SeeVac appears to have gone under or been bought since making the Conductavac IV(~25 years of age) so trying to find a troubleshooting source has been difficult. Any suggestions, whether is specially pertains to the Conductavac IV or any sputter coater, on my amp problem will be greatly appreciated. Thank you for your time.
-Joe
-- Joseph Heintz Lab/BBPIC Manager University of Wisconsin-Madison Room 1048, Animal Sciences Building 1675 Observatory Dr. Madison, WI 53706 608.263.4162
==============================Original Headers============================== 4, 28 -- From josephheintz-at-gmail.com Thu Aug 4 08:21:46 2011 4, 28 -- Received: from mail-ey0-f171.google.com (mail-ey0-f171.google.com [209.85.215.171]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74DLiU2023616 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 08:21:45 -0500 4, 28 -- Received: by eyg24 with SMTP id 24so1587840eyg.30 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 04 Aug 2011 06:21:43 -0700 (PDT) 4, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 28 -- d=gmail.com; s=gamma; 4, 28 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 4, 28 -- :subject:to:content-type:content-transfer-encoding; 4, 28 -- bh=PtNp7rAOGmRk3jijIhul4oVB6DJUFeIOj46Nq6TZ208=; 4, 28 -- b=Q5owK5WdPh3znc1Xt8SknFvMoQnvl6NPt+oe9ogkaU/NFoDkRDbjZo6v2kV7MtEcn2 4, 28 -- 2R6enN196sKdMHnlsBvXIdZbQMgf586aTRaon8Kctj+jMoIdxDs/lH8ZSqKq3ZyT/KMe 4, 28 -- QpyEHo7b8iGwRg5IYiRj5bW1QhTUD6tjJkGU0= 4, 28 -- Received: by 10.213.36.9 with SMTP id r9mr675877ebd.75.1312464103452; Thu, 04 4, 28 -- Aug 2011 06:21:43 -0700 (PDT) 4, 28 -- MIME-Version: 1.0 4, 28 -- Sender: josephheintz-at-gmail.com 4, 28 -- Received: by 10.213.22.200 with HTTP; Thu, 4 Aug 2011 06:21:22 -0700 (PDT) 4, 28 -- From: Joseph Heintz {jheintz-at-wisc.edu} 4, 28 -- Date: Thu, 4 Aug 2011 08:21:22 -0500 4, 28 -- X-Google-Sender-Auth: y8MEp8SMldYp3edC6-SmDVyHDDQ 4, 28 -- Message-ID: {CALFNmMDzxg5XdS5svSu7vmA28oe9+GMpL9fCiAMAZYgRaFvFsg-at-mail.gmail.com} 4, 28 -- Subject: Sputter Coater( Conductavac IV ) Issue 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1 4, 28 -- Content-Transfer-Encoding: 8bit 4, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p74DLiU2023616 ==============================End of - Headers==============================
Check for break-down of the high-temperature epoxy around the electrical feed-through at the top. I had that problem in the past.
Phil
} Hello All, } I am attempting to remedy an issue with a Conductavac IV sputter coater by } See Vac. Recently, it was observed that the ammeter needle was wavering } considerably during the sputter coating process. The needle would be at 0 mA } and with a slight twist of the Current Adjust Knob the needle would be up } near 40mA(~ half way up ammeter). I assumed there might be a short } somewhere in the system and after checking all the connections and making } sure all junctions were snug no obvious problems were observed. } Another sputter coating run was attempted but to no avail. However, the } needle now moves with more fluidity. Control of the needle has been regained } though the needle currently maxes out(100mA) where the needle would } typically be at 30mA(proper amps for general sputter coating). Why the rapid } increase in amps? I have also clean the anode and cathode hoping the } removing any debris would help solve my issue. The vacuum pumping out the } system is working fine and easily pumps below 100mTorr. } SeeVac appears to have gone under or been bought since making the } Conductavac IV(~25 years of age) so trying to find a troubleshooting source } has been difficult. Any suggestions, whether is specially pertains to the } Conductavac IV or any sputter coater, on my amp problem will be greatly } appreciated. Thank you for your time. } } -Joe } } -- } Joseph Heintz } Lab/BBPIC Manager } University of Wisconsin-Madison } Room 1048, Animal Sciences Building } 1675 Observatory Dr. } Madison, WI 53706 } 608.263.4162
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 27 -- From oshel1pe-at-cmich.edu Thu Aug 4 09:31:21 2011 5, 27 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74EVJIw008305 5, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 09:31:20 -0500 5, 27 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 5, 27 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p74EVBbf032484; 5, 27 -- Thu, 4 Aug 2011 10:31:12 -0400 5, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.289.1; Thu, 4 Aug 5, 27 -- 2011 10:31:11 -0400 5, 27 -- MIME-Version: 1.0 5, 27 -- Message-ID: {a06240806ca605d6b3d55-at-[141.209.160.249]} 5, 27 -- In-Reply-To: {201108041330.p74DUUjx028736-at-ns.microscopy.com} 5, 27 -- References: {201108041330.p74DUUjx028736-at-ns.microscopy.com} 5, 27 -- Date: Thu, 4 Aug 2011 10:31:07 -0400 5, 27 -- To: {Microscopy-at-microscopy.com} 5, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 27 -- Subject: Re: [Microscopy] Sputter Coater( Conductavac IV ) Issue 5, 27 -- CC: {jheintz-at-wisc.edu} 5, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 27 -- X-Originating-IP: [141.209.160.249] 5, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 27 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 5, 27 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 27 -- X-CanItPRO-Stream: default 5, 27 -- X-Canit-Stats-ID: 02FgevcTw - e5a6092fa1a1 - 20110804 5, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
My start at troubleshooting would be to determine 1) if the ammeter is at fault or 2) if the current being measured is really fluctuating as the meter seems to indicate. Background: Ammeters are typically voltmeters that measure the voltage drop across a shunt resistor. Then, using Ohms law, the current measured = I = V/R, where V is the voltage drop measured and R is the value of the shunt resistance. The shunt resistor may be external to the meter, inside the meter case, or for very low currents the resistance of the meter coil itself will suffice (there would be no shunt resistor - external or internal -in this instance). Suggested troubleshooting: After rechecking and cleaning any connections to a possible external shunt resistor, I would substitute a good digital volt-ohm-meter (a typical Fluke 112, etc.) for the suspect ammeter, set to read amperage, and see if the fluctuations reproduce. With the results of this test, you should then know whether the meter needs replacing or you need to keep looking elsewhere.
Hope this helps, Rick
Richard A. Ross Sr. Engineer Materials Engineering Allison Transmission, Inc. PO Box 894, Mail M-13 Indianapolis, IN 46206 317-242-4880
-----Original Message----- X-from: jheintz-at-wisc.edu [mailto:jheintz-at-wisc.edu] Sent: Thursday, August 04, 2011 9:38 AM To: Richard A. Ross
Hello All, I am attempting to remedy an issue with a Conductavac IV sputter coater by See Vac. Recently, it was observed that the ammeter needle was wavering considerably during the sputter coating process. The needle would be at 0 mA and with a slight twist of the Current Adjust Knob the needle would be up near 40mA(~ half way up ammeter). I assumed there might be a short somewhere in the system and after checking all the connections and making sure all junctions were snug no obvious problems were observed. Another sputter coating run was attempted but to no avail. However, the needle now moves with more fluidity. Control of the needle has been regained though the needle currently maxes out(100mA) where the needle would typically be at 30mA(proper amps for general sputter coating). Why the rapid increase in amps? I have also clean the anode and cathode hoping the removing any debris would help solve my issue. The vacuum pumping out the system is working fine and easily pumps below 100mTorr. SeeVac appears to have gone under or been bought since making the Conductavac IV(~25 years of age) so trying to find a troubleshooting source has been difficult. Any suggestions, whether is specially pertains to the Conductavac IV or any sputter coater, on my amp problem will be greatly appreciated. Thank you for your time.
-Joe
-- Joseph Heintz Lab/BBPIC Manager University of Wisconsin-Madison Room 1048, Animal Sciences Building 1675 Observatory Dr. Madison, WI 53706 608.263.4162
==============================Original Headers============================== 4, 28 -- From josephheintz-at-gmail.com Thu Aug 4 08:21:46 2011 4, 28 -- Received: from mail-ey0-f171.google.com (mail-ey0-f171.google.com [209.85.215.171]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74DLiU2023616 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 08:21:45 -0500 4, 28 -- Received: by eyg24 with SMTP id 24so1587840eyg.30 4, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 04 Aug 2011 06:21:43 -0700 (PDT) 4, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 28 -- d=gmail.com; s=gamma; 4, 28 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 4, 28 -- :subject:to:content-type:content-transfer-encoding; 4, 28 -- bh=PtNp7rAOGmRk3jijIhul4oVB6DJUFeIOj46Nq6TZ208=; 4, 28 -- b=Q5owK5WdPh3znc1Xt8SknFvMoQnvl6NPt+oe9ogkaU/NFoDkRDbjZo6v2kV7MtEcn2 4, 28 -- 2R6enN196sKdMHnlsBvXIdZbQMgf586aTRaon8Kctj+jMoIdxDs/lH8ZSqKq3ZyT/KMe 4, 28 -- QpyEHo7b8iGwRg5IYiRj5bW1QhTUD6tjJkGU0= 4, 28 -- Received: by 10.213.36.9 with SMTP id r9mr675877ebd.75.1312464103452; Thu, 04 4, 28 -- Aug 2011 06:21:43 -0700 (PDT) 4, 28 -- MIME-Version: 1.0 4, 28 -- Sender: josephheintz-at-gmail.com 4, 28 -- Received: by 10.213.22.200 with HTTP; Thu, 4 Aug 2011 06:21:22 -0700 (PDT) 4, 28 -- From: Joseph Heintz {jheintz-at-wisc.edu} 4, 28 -- Date: Thu, 4 Aug 2011 08:21:22 -0500 4, 28 -- X-Google-Sender-Auth: y8MEp8SMldYp3edC6-SmDVyHDDQ 4, 28 -- Message-ID: {CALFNmMDzxg5XdS5svSu7vmA28oe9+GMpL9fCiAMAZYgRaFvFsg-at-mail.gmail.com} 4, 28 -- Subject: Sputter Coater( Conductavac IV ) Issue 4, 28 -- To: Microscopy-at-microscopy.com 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1 4, 28 -- Content-Transfer-Encoding: 8bit 4, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p74DLiU2023616 ==============================End of - Headers==============================
==============================Original Headers============================== 11, 39 -- From richard.ross-at-allisontransmission.com Thu Aug 4 09:50:37 2011 11, 39 -- Received: from IMR01.allisontransmission.com (imr01.allisontransmission.com [12.53.132.85]) 11, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74EoaO6023084 11, 39 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 09:50:36 -0500 11, 39 -- X-ASG-Debug-ID: 1312471264-13b538e60001-4CH8be 11, 39 -- Received: from USAT0SAW083.US.AD.ATI.INT ([143.242.253.149]) by IMR01.allisontransmission.com with ESMTP id BwZAXaXFpyUjSbnR (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 04 Aug 2011 11:21:04 -0400 (EDT) 11, 39 -- X-Barracuda-Envelope-From: richard.ross-at-allisontransmission.com 11, 39 -- X-Barracuda-Apparent-Source-IP: 143.242.253.149 11, 39 -- Received: from USAT0SAW080.US.AD.ATI.INT ([192.168.1.69]) by 11, 39 -- USAT0SAW083.US.AD.ATI.INT ([192.168.1.182]) with mapi id 14.01.0270.001; Thu, 11, 39 -- 4 Aug 2011 10:50:34 -0400 11, 39 -- From: "Richard A. Ross" {richard.ross-at-allisontransmission.com} 11, 39 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 39 -- Subject: Re: [Microscopy] Sputter Coater( Conductavac IV ) Issue 11, 39 -- Thread-Topic: [Microscopy] Sputter Coater( Conductavac IV ) Issue 11, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] Sputter Coater( Conductavac IV ) Issue 11, 39 -- Thread-Index: AcxStdycYkhpIx//T7uAHIZzq+08IQ== 11, 39 -- Date: Thu, 4 Aug 2011 14:50:33 +0000 11, 39 -- Message-ID: {2BF09958126C994AB7C38B7B1247D94E1312BE14-at-USAT0SAW080} 11, 39 -- Accept-Language: en-US 11, 39 -- Content-Language: en-US 11, 39 -- X-MS-Has-Attach: 11, 39 -- X-MS-TNEF-Correlator: 11, 39 -- x-originating-ip: [10.1.2.196] 11, 39 -- Content-Type: text/plain; charset="iso-8859-1" 11, 39 -- MIME-Version: 1.0 11, 39 -- X-Barracuda-Connect: UNKNOWN[143.242.253.149] 11, 39 -- X-Barracuda-Start-Time: 1312471264 11, 39 -- X-Barracuda-Encrypted: AES128-SHA 11, 39 -- X-Barracuda-URL: http://12.53.132.85:8000/cgi-mod/mark.cgi 11, 39 -- X-Virus-Scanned: by bsmtpd at allisontransmission.com 11, 39 -- X-Barracuda-Spam-Score: 0.00 11, 39 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 11, 39 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.70889 11, 39 -- Rule breakdown below 11, 39 -- pts rule name description 11, 39 -- ---- ---------------------- -------------------------------------------------- 11, 39 -- Content-Transfer-Encoding: 8bit 11, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p74EoaO6023084 ==============================End of - Headers==============================
I have a researcher with a project where the antigen they want to label is naturally present in the control and should be present in a greater amount in the experimental cell culture. Right now the amount of label looks to be similar in both. I'm looking for help on how to proceed to differentiate these two conditions better. If anyone is interested in helping please contact me privately. Thanks.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 3, 23 -- From tbargar-at-unmc.edu Thu Aug 4 11:00:47 2011 3, 23 -- Received: from zixvpm01.unmc.edu (mysecuremail.unmc.edu [192.198.54.126] (may be forged)) 3, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74G0jXt009752 3, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 11:00:46 -0500 3, 23 -- Received: from zixvpm01.unmc.edu (ZixVPM [127.0.0.1]) 3, 23 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 1EC331090482 3, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 11:00:43 -0500 (CDT) 3, 23 -- Received: from unmcnotes01.unmc.edu (unknown [10.8.51.5]) 3, 23 -- by zixvpm01.unmc.edu (Proprietary) with ESMTP id 48A6510904FB 3, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 11:00:42 -0500 (CDT) 3, 23 -- To: Microscopy-at-microscopy.com 3, 23 -- MIME-Version: 1.0 3, 23 -- Subject: Looking for advice about a immuno-gold project 3, 23 -- X-KeepSent: EAF80DC0:B322D5AA-862578E2:00576479; 3, 23 -- type=4; name=$KeepSent 3, 23 -- X-Mailer: Lotus Notes Release 8.0.2 August 07, 2008 3, 23 -- Message-ID: {OFEAF80DC0.B322D5AA-ON862578E2.00576479-862578E2.0057F4FA-at-unmc.edu} 3, 23 -- From: Tom W Bargar {tbargar-at-unmc.edu} 3, 23 -- Date: Thu, 4 Aug 2011 11:00:41 -0500 3, 23 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 08/04/2011 11:00:42 3, 23 -- AM, 3, 23 -- Serialize complete at 08/04/2011 11:00:42 AM 3, 23 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wall1-at-LLNL.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wall1-at-LLNL.gov Name: Mark A. Wall
Organization: LLNL
Title-Subject: [Filtered] Desktop Microscopist
Message: Can anyone tell me if it is still possible to purchase a copy of Desktop Microscopist? If so, from who? My Web searches keep hitting roadblocks. thanks,
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kjl226-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kjl226-at-vt.edu Name: Kathy Lowe
Organization: Virginia Tech
Title-Subject: [Filtered] a successful service lab
Message: How many full time electron microscoptist do you think it would take to run a successful Electron Microscopy Service Lab with the following work load occurring in just 6 months?
1. Processing: 140 TEM samples 2. Processing:107 SEM samples including some CPD and Sputter coating 3. Negative staining: 68 4. Thick section:50 5. Thin sections: 140+ 6. Helping on the SEM and TEM
I would like to add that the samples submitted are mostly biological with some polymer and other materials.
Harvey Hoch is retiring from Cornell and he wants to find a home for his JEOL 100SX TEM. He loves this 20 year old scope and he says it is working fine. He has permission to give it away. The party receiving the scope would be responsible for the expense of having it disassembled and moved. If interested please contact Harvey at hch1-at-cornell.edu as soon as possible.
my best, Beth (I'm just the messenger)
==============================Original Headers============================== 3, 20 -- From beth-at-plantbio.uga.edu Thu Aug 4 16:18:30 2011 3, 20 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 3, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74LIUGI008895 3, 20 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 16:18:30 -0500 3, 20 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 3, 20 -- Received: from [128.192.26.69] ([128.192.26.69]) 3, 20 -- (authenticated user beth-at-plantbio.uga.edu) 3, 20 -- by dogwood.plantbio.uga.edu 3, 20 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 3, 20 -- for microscopy-at-microscopy.com; 3, 20 -- Thu, 4 Aug 2011 17:18:28 -0400 3, 20 -- Message-Id: {72603033-05FB-43F8-A7F8-6717B71656AB-at-plantbio.uga.edu} 3, 20 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 3, 20 -- To: microscopy microscopy {microscopy-at-microscopy.com} 3, 20 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 3, 20 -- Content-Transfer-Encoding: 7bit 3, 20 -- Mime-Version: 1.0 (Apple Message framework v936) 3, 20 -- Subject: JEOL 100SX TEM - free to a good home 3, 20 -- Date: Thu, 4 Aug 2011 17:18:27 -0400 3, 20 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lisa.whitworth-at-okstate.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: Wolfgang Jäger {wj-at-tf.uni-kiel.de}
Organization: Christian-Albrechts-University of Kiel
Microscopy Conference MC 2011 August 28 - September 2, 2011, Kiel / Germany http://www.mc2011.de
Scientists and manufacturers meet at the Bay of Kiel for 6 days, from August 28th to September 2nd, 2011, in order to discuss and exchange the latest developments in Instrumentation and Methods and current topics in Materials Science and Life Science during the International Microscopy Conference 2011.
The venue of the congress is the campus of the Christian-Albrechts-University of Kiel, for the first time. The congress is jointly organized by the DGE German Society for Electron Microscopy in collaboration with the European Microscopy Society (EMS), the Nordic Microscopy Society (SCANDEM), the Polish Microscopy Society (PTMi), and with scientists from research institutions in Estonia, Latvia, Lithuania, and St. Petersburg, Russia.
The congress was initiated by Professor Wolfgang Jaeger of the Faculty of Engineering of the Kiel University and will bring together internationally distinguished scientists with experts from industry. Currently 700 participants from all over the world and 49 companies are expected to meet in Kiel from August 28 until September 2, 2011. The scientific program of the congress, designed by an international panel of distinguished scientists, focuses on electron microscopy and related methods and comprises more than 25 symposia and workshops. The program is complemented by plenary talks of leading scientists on current developments of Instrumentation and Methods and on current topics in Materials Science and in Life Science.
The conference is actively supported by the Helmholtz Zentrum Geesthacht (HZG) Centre for Materials and Coastal Research and by the Fraunhofer-Institute for Silicon Technology (ISIT) Itzehoe.
In addition, the conference will provide a forum for the information exchange between scientists and manufacturers, as it will host a major trade exhibition, bringing together manufacturers of microscopes and suppliers of equipment and products in all fields of microscopy and related techniques.
Wolfgang Jaeger Professor, Dr. Mikrostrukturanalytik, Institut für Materialwissenschaft Microanalysis of Materials, Institute for Materials Science Christian-Albrechts-Universitaet zu Kiel Kaiserstrasse 2 24143 Kiel Germany EU T +49 431 880 6177 F -6178 wj-at-tf.uni-kiel.de Chairman MC2011 Microscopy Conference Kiel Germany www.mc2011.de
==============================Original Headers============================== 14, 31 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 4 16:22:49 2011 14, 31 -- Received: from mx0a-000f2801.pphosted.com (mx0a-000f2801.pphosted.com [67.231.144.139]) 14, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74LMmmj024834 14, 31 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 16:22:48 -0500 14, 31 -- Received: from pps.filterd (m0000510 [127.0.0.1]) 14, 31 -- by m0000510.ppops.net (8.14.3/8.14.3) with SMTP id p74LK9O4024052 14, 31 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 17:22:48 -0400 14, 31 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) 14, 31 -- by mx0a-000f2801.pphosted.com with ESMTP id xyta1dg2v-1 14, 31 -- for {microscopy-at-microscopy.com} ; Thu, 04 Aug 2011 17:22:47 -0400 14, 31 -- Received: from NestorPwrBk.local ([192.168.13.114]) 14, 31 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p74LMlqx063175 14, 31 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 21:22:47 GMT 14, 31 -- Message-ID: {4E3B0DA7.60901-at-microscopy.com} 14, 31 -- Date: Thu, 04 Aug 2011 16:22:47 -0500 14, 31 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 31 -- Reply-To: =?ISO-8859-1?Q?Wolfgang_J=E4ger?= {wj-at-tf.uni-kiel.de} 14, 31 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 14, 31 -- MIME-Version: 1.0 14, 31 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 31 -- Subject: viaWWW: MC 2011 Meeting Aug 28-Sept 2 14, 31 -- X-Priority: 1 (Highest) 14, 31 -- References: {4DCAFCE0.1010000-at-microscopy.com} {9CF7044DB7A24752B77DDE462EB5D075-at-rifleman} {4E3AB07C.9050401-at-microscopy.com} 14, 31 -- In-Reply-To: {4E3AB07C.9050401-at-microscopy.com} 14, 31 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 14, 31 -- definitions=2011-08-04_05:2011-08-04,2011-08-04,1970-01-01 signatures=0 14, 31 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=2 14, 31 -- phishscore=0 bulkscore=42 adultscore=0 classifier=spam adjust=0 reason=mlx 14, 31 -- engine=6.0.2-1012030000 definitions=main-1108040224 ==============================End of - Headers==============================
At the moment, Desktop Microscopist is essentially defunct. The person who was doing the development has pretty much dropped the project.
A few weeks back, Matt Olszta (matthew.olszta-at-pnnl.gov) posted a message to the listserver asking about interest in reviving Desktop Microscopist. He might be a good contact to see if anyone else is interested.
Cheers, Henk
At 8/4/2011 5:14 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both wall1-at-LLNL.gov as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: wall1-at-LLNL.gov Name: Mark A. Wall } } Organization: LLNL } } Title-Subject: [Filtered] Desktop Microscopist } } Message: Can anyone tell me if it is still possible to purchase a copy } of Desktop Microscopist? If so, from who? My Web searches keep hitting } roadblocks. } thanks, } } Mark A. Wall } LLNL } } Login Host: 128.115.27.11 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 4 16:13:32 2011 } 9, 28 -- Received: from mx0a-000f2801.pphosted.com (mx0a-000f2801.pphosted.com [67.231.144.139]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74LDWZ1029551 } 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 16:13:32 -0500 } 9, 28 -- Received: from pps.filterd (m0000510 [127.0.0.1]) } 9, 28 -- by m0000510.ppops.net (8.14.3/8.14.3) with SMTP id p74LB6WD017157 } 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 17:13:31 -0400 } 9, 28 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) } 9, 28 -- by mx0a-000f2801.pphosted.com with ESMTP id xyta1df0x-1 } 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 04 Aug 2011 17:13:26 -0400 } 9, 28 -- Received: from NestorPwrBk.local ([192.168.13.114]) } 9, 28 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p74LDMb4053208 } 9, 28 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 21:13:22 GMT } 9, 28 -- Message-ID: {4E3B0B72.7040502-at-microscopy.com} } 9, 28 -- Date: Thu, 04 Aug 2011 16:13:22 -0500 } 9, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 9, 28 -- Reply-To: wall1-at-LLNL.gov } 9, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 28 -- Subject: [Filtered] MicroscopyListserverviaWWW: Looking for Desktop Microscopist } 9, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 28 -- Content-Transfer-Encoding: 7bit } 9, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 } 9, 28 -- definitions=2011-08-04_05:2011-08-04,2011-08-04,1970-01-01 signatures=0 } 9, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 } 9, 28 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx } 9, 28 -- engine=6.0.2-1012030000 definitions=main-1108040221 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 7, 27 -- From colijn.1-at-osu.edu Thu Aug 4 18:52:28 2011 7, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p74NqST9031848 7, 27 -- for {microscopy-at-microscopy.com} ; Thu, 4 Aug 2011 18:52:28 -0500 7, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 7, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 7, 27 -- id {01O4GQ4EUJ749J44H4-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 7, 27 -- Thu, 04 Aug 2011 19:52:25 -0400 (EDT) 7, 27 -- Received: from [192.168.1.103] 7, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 7, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 7, 27 -- with ESMTPA id {01O4GQ4EHWPK9ENZD6-at-ecr6.ohio-state.edu} for 7, 27 -- microscopy-at-microscopy.com; Thu, 04 Aug 2011 19:52:25 -0400 (EDT) 7, 27 -- Date: Thu, 04 Aug 2011 19:52:25 -0400 7, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 7, 27 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Looking for 7, 27 -- Desktop Microscopist 7, 27 -- In-reply-to: {201108042114.p74LEfc9031414-at-ns.microscopy.com} 7, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 7, 27 -- To: microscopy-at-microscopy.com 7, 27 -- Message-id: {4E3B30B9.4050806-at-osu.edu} 7, 27 -- MIME-version: 1.0 7, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 7, 27 -- Content-transfer-encoding: 7bit 7, 27 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 7, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 7, 27 -- References: {201108042114.p74LEfc9031414-at-ns.microscopy.com} ==============================End of - Headers==============================
We need to get a TEM plasma cleaner in our lab, but we have some restrictions on our budget. The plasma cleaner should be suitable for TEM holders, and both soft and hard materials. We are thinking about a FEMTO UHP Plasma Treatment System (from Diener electronic) or the SPI Plasma Prep Plasma Cleaner. Does anyone have those plasma cleaners in ther lab? If yes, could you provide some feedback?
Best Regards,
Ana
==============================Original Headers============================== 4, 28 -- From contact-at-integrityscientific.com Fri Aug 5 03:19:45 2011 4, 28 -- Received: from mail-relay-1.csv.warwick.ac.uk (mail-relay-1.csv.warwick.ac.uk [137.205.128.7]) 4, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p758Ji27020803 4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 03:19:45 -0500 4, 28 -- Received: from localhost (localhost [127.0.0.1]) 4, 28 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p758JiLF065744 4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 09:19:44 +0100 (BST) 4, 28 -- X-Virus-Scanned: amavisd-new at warwick.ac.uk 4, 28 -- Received: from mail-relay-1.csv.warwick.ac.uk ([127.0.0.1]) 4, 28 -- by localhost (localhost [127.0.0.1]) (amavisd-new, port 10024) 4, 28 -- with LMTP id cAoCYaifldpn for {microscopy-at-microscopy.com} ; 4, 28 -- Fri, 5 Aug 2011 09:19:43 +0100 (BST) 4, 28 -- Received: from [127.0.0.1] (bogwort [137.205.160.5]) 4, 28 -- by mail-relay-1.csv.warwick.ac.uk (8.13.8/8.13.6) with ESMTP id p758Jh9A065715 4, 28 -- for {microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 09:19:43 +0100 (BST) 4, 28 -- X-Envelope-From: contact-at-integrityscientific.com 4, 28 -- Message-ID: {4E3BA79E.5050702-at-integrityscientific.com} 4, 28 -- Date: Fri, 05 Aug 2011 09:19:42 +0100 4, 28 -- From: Richard Beanland {contact-at-integrityscientific.com} 4, 28 -- Reply-To: contact-at-integrityscientific.com 4, 28 -- Organization: Integrity Scientific Ltd 4, 28 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:5.0) Gecko/20110624 Thunderbird/5.0 4, 28 -- MIME-Version: 1.0 4, 28 -- To: microscopy-at-microscopy.com 4, 28 -- Subject: TEM plasma cleaner 4, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 28 -- Content-Transfer-Encoding: 7bit 4, 28 -- X-DCC-Warwick-Metrics: bluebell 1332; Body=1 Fuz1=1 Fuz2=1 ==============================End of - Headers==============================
Dear please consider that the OWLS 2012 website is open even if under "work in progress". Please, put on the agenda the dates. If you are interested un bursaries please let me know via email using as subject "OWLS12 support", deadline is "asap". All the best Alby
==============================Original Headers============================== 1, 24 -- From sekkio-at-mac.com Fri Aug 5 07:29:01 2011 1, 24 -- Received: from asmtpout029.mac.com (asmtpout029.mac.com [17.148.16.104]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p75CT0dV012023 1, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 07:29:01 -0500 1, 24 -- MIME-version: 1.0 1, 24 -- Content-transfer-encoding: 7BIT 1, 24 -- Content-type: text/plain; CHARSET=US-ASCII 1, 24 -- Received: from [172.20.10.3] (unknown [109.54.212.161]) 1, 24 -- by asmtp029.mac.com (Oracle Communications Messaging Exchange Server 7u4-20.01 1, 24 -- 64bit (built Nov 21 2010)) with ESMTPSA id {0LPG004KEFZSYS60-at-asmtp029.mac.com} 1, 24 -- for Microscopy-at-microscopy.com; Fri, 05 Aug 2011 05:28:45 -0700 (PDT) 1, 24 -- X-Proofpoint-Virus-Version: vendor=fsecure 1, 24 -- engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 1, 24 -- definitions=2011-08-05_04:2011-08-05,2011-08-05,1970-01-01 signatures=0 1, 24 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 1, 24 -- ipscore=0 suspectscore=0 phishscore=0 bulkscore=0 adultscore=0 classifier=spam 1, 24 -- adjust=0 reason=mlx engine=6.0.2-1012030000 definitions=main-1108050065 1, 24 -- From: al {sekkio-at-mac.com} 1, 24 -- Subject: alby about owls2012 1, 24 -- Date: Fri, 05 Aug 2011 14:28:40 +0200 1, 24 -- Message-id: {A09C2A7C-8613-44C8-9912-B1BECB0074DE-at-mac.com} 1, 24 -- To: Confocal Microscopy List {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} , 1, 24 -- Microscopy-at-microscopy.com 1, 24 -- X-Mailer: Apple Mail (2.1244.3) ==============================End of - Headers==============================
I know that this isn't exactly microscopy-related but I have a question about fitting x-ray reflectivity data.
All of the programs I've used for fitting thin film x-ray reflectivity (GenX, SimulReflec, ReflPak) require three columns of data: q, reflectivity, and counting error dR. I've been measuring some samples on a Rigaku SmartLab system and it only outputs two columns: q and counts. I know how to normalize the counts to the critical edge to get reflectivity, but how do I extract the error dR? Is it something that the x-ray system has to record during a scan?
I have a feeling it's something simple that I slept through in my statistics class but any help would be greatly appreciated. Thanks!
-- Steven Spurgeon Dynamic Characterization Group Department of Materials Science & Engineering Drexel University
If the count rate is genuinely the number of photons detected (N), then the count rate is Poisson distributed, i.e. the standard deviation = sqrt(N) to a very good approximation. The approximation gets better the higher the count rate is; I would use this approximation only if N} 10 counts/channel.
Yours, Jon
} Hi listers, } } I know that this isn't exactly microscopy-related but I have a } question about fitting x-ray reflectivity data. } } All of the programs I've used for fitting thin film x-ray reflectivity } (GenX, SimulReflec, ReflPak) require three columns of data: q, } reflectivity, and counting error dR. I've been measuring some samples } on a Rigaku SmartLab system and it only outputs two columns: q and } counts. I know how to normalize the counts to the critical edge to get } reflectivity, but how do I extract the error dR? Is it something that } the x-ray system has to record during a scan? } } I have a feeling it's something simple that I slept through in my } statistics class but any help would be greatly appreciated. Thanks! } } -- } Steven Spurgeon } Dynamic Characterization Group } Department of Materials Science & Engineering } Drexel University } } Office: Bossone 105 } Email: spurgeon-at-drexel.edu } Web: http://www.materials.drexel.edu/dcg/ } } ==============================Original } Headers============================== 6, 23 -- From spurgeon-at-drexel.edu } Fri Aug 5 11:10:28 2011 6, 23 -- Received: from smtp.mail.drexel.edu } (pm2.irt.drexel.edu [144.118.29.82]) 6, 23 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id p75GASH6015147 6, 23 -- for } {Microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 11:10:28 -0500 6, 23 -- } Received: from smtp.mail.drexel.edu (localhost.localdomain [127.0.0.1]) } 6, 23 -- by smtp.mail.drexel.edu (Postfix) with SMTP id EAB34116947 6, 23 } -- for {Microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 12:10:27 -0400 (EDT) } 6, 23 -- Received: from mail-iy0-f169.google.com } (mail-iy0-f169.google.com [209.85.210.169]) 6, 23 -- by } smtp.mail.drexel.edu (Postfix) with ESMTP id C09991168A0 6, 23 -- for } {Microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 12:10:27 -0400 (EDT) 6, 23 } -- Received: by iym1 with SMTP id 1so3756212iym.0 6, 23 -- for } {Microscopy-at-microscopy.com} ; Fri, 05 Aug 2011 09:10:27 -0700 (PDT) 6, 23 } -- Received: by 10.43.43.129 with SMTP id } uc1mr811279icb.72.1312560627378; Fri, 6, 23 -- 05 Aug 2011 09:10:27 -0700 } (PDT) 6, 23 -- MIME-Version: 1.0 6, 23 -- Received: by 10.42.219.133 with } HTTP; Fri, 5 Aug 2011 09:10:07 -0700 (PDT) 6, 23 -- From: Steven Spurgeon } {spurgeon-at-drexel.edu} 6, 23 -- Date: Fri, 5 Aug 2011 12:10:07 -0400 6, 23 } -- Message-ID: } {CAL9U+jyef+MtSowsuFB=suk6-dwU+OxW6jq1udr_UiZgiBhUsg-at-mail.gmail.com} 6, } 23 -- Subject: Fitting x-ray reflectivity data 6, 23 -- To: } Microscopy-at-microscopy.com 6, 23 -- Content-Type: text/plain; } charset=ISO-8859-1 6, 23 -- X-PerlMx-Authed: User SMTP Authed } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 28 -- From jsb43-at-hermes.cam.ac.uk Fri Aug 5 12:35:53 2011 6, 28 -- Received: from ppsw-41.csi.cam.ac.uk (ppsw-41.csi.cam.ac.uk [131.111.8.141]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p75HZqTo000543 6, 28 -- for {Microscopy-at-microscopy.com} ; Fri, 5 Aug 2011 12:35:53 -0500 6, 28 -- X-Cam-AntiVirus: no malware found 6, 28 -- X-Cam-SpamDetails: not scanned 6, 28 -- X-Cam-ScannerInfo: http://www.cam.ac.uk/cs/email/scanner/ 6, 28 -- Received: from hermes-2.csi.cam.ac.uk ([131.111.8.54]:53434) 6, 28 -- by ppsw-41.csi.cam.ac.uk (smtp.hermes.cam.ac.uk [131.111.8.156]:25) 6, 28 -- with esmtpa (EXTERNAL:jsb43) id 1QpOJM-0005wH-RB (Exim 4.72) 6, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Aug 2011 18:35:52 +0100 6, 28 -- Received: from prayer by hermes-2.csi.cam.ac.uk (hermes.cam.ac.uk) 6, 28 -- with local (PRAYER:jsb43) id 1QpOJM-0006KD-DK (Exim 4.67) 6, 28 -- (return-path {jsb43-at-hermes.cam.ac.uk} ); Fri, 05 Aug 2011 18:35:52 +0100 6, 28 -- Received: from [131.111.102.31] by webmail.hermes.cam.ac.uk 6, 28 -- with HTTP (Prayer-1.3.4); 05 Aug 2011 18:35:52 +0100 6, 28 -- Date: 05 Aug 2011 18:35:52 +0100 6, 28 -- From: "J.S. Barnard" {jsb43-at-cam.ac.uk} 6, 28 -- To: spurgeon-at-drexel.edu 6, 28 -- Cc: MSA Listserver {Microscopy-at-microscopy.com} 6, 28 -- Subject: Re: [Microscopy] Fitting x-ray reflectivity data 6, 28 -- Message-ID: {Prayer.1.3.4.1108051835520.20282-at-hermes-2.csi.cam.ac.uk} 6, 28 -- In-Reply-To: {201108051615.p75GFkAS021368-at-ns.microscopy.com} 6, 28 -- References: {201108051615.p75GFkAS021368-at-ns.microscopy.com} 6, 28 -- X-Mailer: Prayer v1.3.4 6, 28 -- Mime-Version: 1.0 6, 28 -- Content-Type: text/plain; format=flowed; charset=ISO-8859-1 6, 28 -- Sender: "J.S. Barnard" {jsb43-at-hermes.cam.ac.uk} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both DougM-at-gmaol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: DougM-at-gmaol.com Name: Doug
Organization: IEFAS Inc
Title-Subject: [Filtered] Room Design
Message: Hello,
We're in the process of building a SEM room and were asked what color do we want our walls to be. I figured this would be a good place to get some opinions. Is there an ideal color for a SEM room? Does one color lend itself to better imaging then another? Thanks all!
Hi Doug, if you do coloured SEM work like me :-) I would propose an "Ansel Adams" neutral grey. Images on the wall will look perfect that way. I would add some spotlights also, if you will be the proud owner of a new scope. :-))
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 06.08.11 07:20, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both DougM-at-gmaol.com as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: DougM-at-gmaol.com Name: Doug } } Organization: IEFAS Inc } } Title-Subject: [Filtered] Room Design } } Message: Hello, } } We're in the process of building a SEM room and were asked what color do } we want our walls to be. I figured this would be a good place to get } some opinions. Is there an ideal color for a SEM room? Does one color } lend itself to better imaging then another? } Thanks all! } } Login Host: 152.135.235.188 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 9, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Aug 6 00:14:08 2011 } 9, 28 -- Received: from mx0a-000f2801.pphosted.com (mx0a-000f2801.pphosted.com [67.231.144.139]) } 9, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p765E8Nn024704 } 9, 28 -- for {microscopy-at-microscopy.com} ; Sat, 6 Aug 2011 00:14:08 -0500 } 9, 28 -- Received: from pps.filterd (m0000510 [127.0.0.1]) } 9, 28 -- by m0000510.ppops.net (8.14.3/8.14.3) with SMTP id p765B1p5005961 } 9, 28 -- for {microscopy-at-microscopy.com} ; Sat, 6 Aug 2011 01:14:07 -0400 } 9, 28 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) } 9, 28 -- by mx0a-000f2801.pphosted.com with ESMTP id xyta1ubpy-1 } 9, 28 -- for {microscopy-at-microscopy.com} ; Sat, 06 Aug 2011 01:14:07 -0400 } 9, 28 -- Received: from NestorPwrBk.local ([192.168.13.114]) } 9, 28 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p765E6jQ090418 } 9, 28 -- for {microscopy-at-microscopy.com} ; Sat, 6 Aug 2011 05:14:06 GMT } 9, 28 -- Message-ID: {4E3CCD9E.5060704-at-microscopy.com} } 9, 28 -- Date: Sat, 06 Aug 2011 00:14:06 -0500 } 9, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 9, 28 -- Reply-To: DougM-at-gmaol.com } 9, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 9, 28 -- MIME-Version: 1.0 } 9, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 28 -- Subject: viaWWW:Room Design } 9, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 28 -- Content-Transfer-Encoding: 7bit } 9, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 } 9, 28 -- definitions=2011-08-06_01:2011-08-05,2011-08-06,1970-01-01 signatures=0 } 9, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 } 9, 28 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx } 9, 28 -- engine=6.0.2-1012030000 definitions=main-1108050255 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 22 -- From stefan.diller-at-t-online.de Sun Aug 7 12:47:35 2011 6, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de [194.25.134.17]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p77HlZ7h009829 6, 22 -- for {microscopy-at-microscopy.com} ; Sun, 7 Aug 2011 12:47:35 -0500 6, 22 -- Received: from fwd09.aul.t-online.de (fwd09.aul.t-online.de ) 6, 22 -- by mailout02.t-online.de with smtp 6, 22 -- id 1Qq7Rm-0000P2-BW; Sun, 07 Aug 2011 19:47:34 +0200 6, 22 -- Received: from mac-pro.speedport.ip (Gh2kA8ZFgh-yoDm0CxoNvaGjaaawyL8ibqkUhqtwVeyMGZx8Fu24T356JhUMZlPQPh-at-[93.222.93.79]) by fwd09.t-online.de 6, 22 -- with esmtp id 1Qq7Rg-1hO88W0; Sun, 7 Aug 2011 19:47:28 +0200 6, 22 -- Message-ID: {4E3ECFB0.7040605-at-t-online.de} 6, 22 -- Date: Sun, 07 Aug 2011 19:47:28 +0200 6, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:5.0) Gecko/20110624 Thunderbird/5.0 6, 22 -- MIME-Version: 1.0 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] viaWWW:Room Design 6, 22 -- References: {201108060520.p765KE5h030718-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {201108060520.p765KE5h030718-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-ID: Gh2kA8ZFgh-yoDm0CxoNvaGjaaawyL8ibqkUhqtwVeyMGZx8Fu24T356JhUMZlPQPh 6, 22 -- X-TOI-MSGID: 28fcdf03-319c-485b-ae92-d7d51b31c865 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Looking for supplier(s) in the UK or Europe of the diamond knife “Drukker international”. The one I inherited was from EMITECH LTD, but was told no longer supply them. The knife is mainly for room temperature TEM sectioning polymer composites. Type: Ultra with trough 2mm-45°. Agar supplies “Diatome”. Attempted to try one of equivalent functions. How do you compare these two knives?
The Company: Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity: The company currently has an opening for a Product Specialist - Microscopy to be based in Chicago IL or Philadelphia PA. Relocation may be considered for this opportunity.
Base: Commensurate with experience Other: Full benefits - 401k program/matching
Primary Responsibilities: This position requires interfacing with customers and sales/applications specialist to deeply understand and provide extensive application support on issues related to Widefield and Advanced Fluorescence Microscopy. Significant time will be spent working with customers to identify optimal solutions to their needs and troubleshoot application, hardware and software problems. In addition, candidate will support trade shows and training courses, including planning, communicating, attending and reporting. Handling demonstration system logistics and confirming correct system functionality is also required.
Education and Experience Required: - Minimum BA/BS in Biological Sciences or Engineering; Advanced degree preferred - Business aptitude required - Familiarity with biological imaging applications needed - Fluorescence microscopy experience highly valued - Good written and verbal communication skills are necessary
If you meet and/or exceed the experience criteria, please submit your resume by emailing Christy Edwards (Sr. e-Recruitment Consultant with Personify) at ce-at-personifysearch.com. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
==============================Original Headers============================== 8, 21 -- From ce-at-personifysearch.com Tue Aug 9 09:10:25 2011 8, 21 -- Received: from na3sys010aob108.obsmtp.com (na3sys010aob108.obsmtp.com [74.125.245.83]) 8, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p79EAOU3014425 8, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Aug 2011 09:10:24 -0500 8, 21 -- Received: from mail-pz0-f41.google.com ([209.85.210.41]) (using TLSv1) by na3sys010aob108.postini.com ([74.125.244.12]) with SMTP 8, 21 -- ID DSNKTkE/yxqEgPPsv5IQ2Yy6Xo7r+VjFvYG9-at-postini.com; Tue, 09 Aug 2011 07:10:22 PDT 8, 21 -- Received: by mail-pz0-f41.google.com with SMTP id 4so42516pzk.28 8, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 09 Aug 2011 07:10:19 -0700 (PDT) 8, 21 -- Received: by 10.143.67.7 with SMTP id u7mr7017603wfk.339.1312899019404; Tue, 8, 21 -- 09 Aug 2011 07:10:19 -0700 (PDT) 8, 21 -- From: Christy Edwards {ce-at-personifysearch.com} 8, 21 -- MIME-Version: 1.0 8, 21 -- X-Mailer: Microsoft Office Outlook 12.0 8, 21 -- Thread-Index: AcxWnhEi8mC9+FXERQmv8/+Ve/4vIg== 8, 21 -- Date: Tue, 9 Aug 2011 10:10:17 -0400 8, 21 -- Message-ID: {3c74d6c1562d5652d21eee31b0c02dca-at-mail.gmail.com} 8, 21 -- Subject: Product Specialist Opportunity with a Market Leader in Microscopy 8, 21 -- To: Microscopy-at-microscopy.com 8, 21 -- Content-Type: text/plain; charset=ISO-8859-1 8, 21 -- Content-Transfer-Encoding: 8bit 8, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p79EAOU3014425 ==============================End of - Headers==============================
We have a Gatan camera showing some unusall features on the gain reference images (those "honeycomb" images). These features, according to Gatan, are perhaps "physical artifacts" on the phosphor coating. They also claim that the scintillator cannot be made defect-free. As long as those artifacts do not affect image quality, there is no need to replace the scintillator - as the new one may have its own problem.
Has anyone met a similar problem? What would be the long-term effects of the physical artifacts (of the phosphor coating) on the performance of the camera? Right now, these artifacts can be corrected by software method (gain reference corrected). However, will they lead to dead pixels eventually?
I should mention that these artifacts are not oil contaminants. We baked the camera several times and they are still there. They are not hardened oil contaminants either. A service engineer from Gatan had cleaned the surface with ethanol. But those features remain on the uncorrected images. Their latest explanation is they might be the "physical artifacts on the phosphor coating".
Any input is welcome. Also, if you would like to see how those artifacts look like, please send me an email.
Kind regards,
Hongwen Zhou
Temple University 1901 N 13th Street Philadelphia, PA 19122 Tel: (215) 204-0366
==============================Original Headers============================== 9, 23 -- From zhouhw33-at-gmail.com Tue Aug 9 13:44:46 2011 9, 23 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 9, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p79IijEV002673 9, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Aug 2011 13:44:45 -0500 9, 23 -- Received: by iym1 with SMTP id 1so466422iym.0 9, 23 -- for {Microscopy-at-microscopy.com} ; Tue, 09 Aug 2011 11:44:45 -0700 (PDT) 9, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 23 -- d=gmail.com; s=gamma; 9, 23 -- h=mime-version:date:message-id:subject:from:to:content-type; 9, 23 -- bh=zr/ax72nq2qrIHBgEUesZi83cfWOFBFnfBM1czrzCyk=; 9, 23 -- b=SjPDbHrCaKJPp3GlX2W/xjrB9/M0rK9Zxiz8vBe7sEr0d2NFTJ4UIIrvR3qObrkNAE 9, 23 -- NDYyQ5I9xDOSpawj7JDFEg4OGcmAvG942kkffUhcGI7OiMvjqCiRknh/7Umdozumf3kl 9, 23 -- WGRz4r257Y+4b0AxNBb6wBhSxPP9buQgA7oyY= 9, 23 -- MIME-Version: 1.0 9, 23 -- Received: by 10.42.146.138 with SMTP id j10mr5434599icv.105.1312915485194; 9, 23 -- Tue, 09 Aug 2011 11:44:45 -0700 (PDT) 9, 23 -- Received: by 10.231.19.204 with HTTP; Tue, 9 Aug 2011 11:44:45 -0700 (PDT) 9, 23 -- Date: Tue, 9 Aug 2011 14:44:45 -0400 9, 23 -- Message-ID: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} 9, 23 -- Subject: Artifacts of the scintillator on a Gatan camera 9, 23 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} 9, 23 -- To: Microscopy-at-microscopy.com 9, 23 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
It seems that my words had caused some confusion. To clarify: when I say "artifacts", I did not refer to the honeycomb pattern. The artifacts are superimposed on the honeycomb pattern.
On Tue, Aug 9, 2011 at 2:44 PM, Hongwen Zhou {zhouhw33-at-gmail.com} wrote: } Dear Listers, } } We have a Gatan camera showing some unusall features on the gain } reference images (those "honeycomb" images). These features, according } to Gatan, are perhaps "physical artifacts" on the phosphor coating. } They also claim that the scintillator cannot be made defect-free. As } long as those artifacts do not affect image quality, there is no need } to replace the scintillator - as the new one may have its own problem. } } Has anyone met a similar problem? What would be the long-term effects } of the physical artifacts (of the phosphor coating) on the performance } of the camera? Right now, these artifacts can be corrected by software } method (gain reference corrected). However, will they lead to dead } pixels eventually? } } I should mention that these artifacts are not oil contaminants. We } baked the camera several times and they are still there. They are not } hardened oil contaminants either. A service engineer from Gatan had } cleaned the surface with ethanol. But those features remain on the } uncorrected images. Their latest explanation is they might be the } "physical artifacts on the phosphor coating". } } Any input is welcome. Also, if you would like to see how those } artifacts look like, please send me an email. } } Kind regards, } } Hongwen Zhou } } } Temple University } 1901 N 13th Street } Philadelphia, PA 19122 } Tel: (215) 204-0366 }
==============================Original Headers============================== 4, 26 -- From zhouhw33-at-gmail.com Tue Aug 9 15:01:43 2011 4, 26 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p79K1h0G020998 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Aug 2011 15:01:43 -0500 4, 26 -- Received: by iym1 with SMTP id 1so83411iym.0 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 09 Aug 2011 13:01:42 -0700 (PDT) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=gamma; 4, 26 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 4, 26 -- :content-type; 4, 26 -- bh=ZzBQoYx23CWnoxkV++iqGtAkVt8yxMmiZkuM0Hii+jI=; 4, 26 -- b=XOu7M1fd8Ld/rgbHIklgOmDr3nc5QxnG1W3ZIC74fVSwyWtrxQc5i0KsPmN344suag 4, 26 -- 3A7o/34jagFXVgAtRgQBzQCkeczts+aUJcuzBsWoncDbyezRFrV5csM2szbwqBCPL5dg 4, 26 -- NtkQxHNAyv2Yfvz4fjD2IL/p3hFCDvW648fwU= 4, 26 -- MIME-Version: 1.0 4, 26 -- Received: by 10.42.149.69 with SMTP id u5mr790154icv.38.1312920102529; Tue, 09 4, 26 -- Aug 2011 13:01:42 -0700 (PDT) 4, 26 -- Received: by 10.231.19.204 with HTTP; Tue, 9 Aug 2011 13:01:42 -0700 (PDT) 4, 26 -- In-Reply-To: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} 4, 26 -- References: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} 4, 26 -- Date: Tue, 9 Aug 2011 16:01:42 -0400 4, 26 -- Message-ID: {CAODik+v-bVBfHSKtXzO=dVs9X8WTsmhc80O3PwsADgzpOmhr7A-at-mail.gmail.com} 4, 26 -- Subject: Re: Artifacts of the scintillator on a Gatan camera 4, 26 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
It is difficult to picture what you mean by "artefacts" without an image. Would it be possible for you to host the image on a website and put a link here, so that we can see what you are talking about? I have to admit that I was also one of the people thinking that you were worried by the honeycomb effect of the fibre optics.
TobyS
Dr Tobias Starborg Senior Experimental Officer: 3DEM Michael Smith Building Oxford Road Manchester M13 9PT Tel: +44(0)1612755170 http://manchesterpolara.org.uk
________________________________________ X-from: zhouhw33-at-gmail.com [zhouhw33-at-gmail.com] Sent: 09 August 2011 21:08 To: Tobias Starborg
It seems that my words had caused some confusion. To clarify: when I say "artifacts", I did not refer to the honeycomb pattern. The artifacts are superimposed on the honeycomb pattern.
On Tue, Aug 9, 2011 at 2:44 PM, Hongwen Zhou {zhouhw33-at-gmail.com} wrote: } Dear Listers, } } We have a Gatan camera showing some unusall features on the gain } reference images (those "honeycomb" images). These features, according } to Gatan, are perhaps "physical artifacts" on the phosphor coating. } They also claim that the scintillator cannot be made defect-free. As } long as those artifacts do not affect image quality, there is no need } to replace the scintillator - as the new one may have its own problem. } } Has anyone met a similar problem? What would be the long-term effects } of the physical artifacts (of the phosphor coating) on the performance } of the camera? Right now, these artifacts can be corrected by software } method (gain reference corrected). However, will they lead to dead } pixels eventually? } } I should mention that these artifacts are not oil contaminants. We } baked the camera several times and they are still there. They are not } hardened oil contaminants either. A service engineer from Gatan had } cleaned the surface with ethanol. But those features remain on the } uncorrected images. Their latest explanation is they might be the } "physical artifacts on the phosphor coating". } } Any input is welcome. Also, if you would like to see how those } artifacts look like, please send me an email. } } Kind regards, } } Hongwen Zhou } } } Temple University } 1901 N 13th Street } Philadelphia, PA 19122 } Tel: (215) 204-0366 }
==============================Original Headers============================== 4, 26 -- From zhouhw33-at-gmail.com Tue Aug 9 15:01:43 2011 4, 26 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p79K1h0G020998 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Aug 2011 15:01:43 -0500 4, 26 -- Received: by iym1 with SMTP id 1so83411iym.0 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 09 Aug 2011 13:01:42 -0700 (PDT) 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 26 -- d=gmail.com; s=gamma; 4, 26 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 4, 26 -- :content-type; 4, 26 -- bh=ZzBQoYx23CWnoxkV++iqGtAkVt8yxMmiZkuM0Hii+jI=; 4, 26 -- b=XOu7M1fd8Ld/rgbHIklgOmDr3nc5QxnG1W3ZIC74fVSwyWtrxQc5i0KsPmN344suag 4, 26 -- 3A7o/34jagFXVgAtRgQBzQCkeczts+aUJcuzBsWoncDbyezRFrV5csM2szbwqBCPL5dg 4, 26 -- NtkQxHNAyv2Yfvz4fjD2IL/p3hFCDvW648fwU= 4, 26 -- MIME-Version: 1.0 4, 26 -- Received: by 10.42.149.69 with SMTP id u5mr790154icv.38.1312920102529; Tue, 09 4, 26 -- Aug 2011 13:01:42 -0700 (PDT) 4, 26 -- Received: by 10.231.19.204 with HTTP; Tue, 9 Aug 2011 13:01:42 -0700 (PDT) 4, 26 -- In-Reply-To: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} 4, 26 -- References: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} 4, 26 -- Date: Tue, 9 Aug 2011 16:01:42 -0400 4, 26 -- Message-ID: {CAODik+v-bVBfHSKtXzO=dVs9X8WTsmhc80O3PwsADgzpOmhr7A-at-mail.gmail.com} 4, 26 -- Subject: Re: Artifacts of the scintillator on a Gatan camera 4, 26 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} 4, 26 -- To: Microscopy-at-microscopy.com 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
==============================Original Headers============================== 13, 32 -- From tobias.starborg-at-manchester.ac.uk Wed Aug 10 03:32:34 2011 13, 32 -- Received: from tranquility.mcc.ac.uk (tranquility.mcc.ac.uk [130.88.200.145]) 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7A8WYFf017796 13, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 03:32:34 -0500 13, 32 -- Received: from casp10.ds.man.ac.uk ([10.2.172.12]) 13, 32 -- by tranquility.mcc.ac.uk with esmtps (TLSv1:AES128-SHA:128) 13, 32 -- (Exim 4.76 (FreeBSD)) 13, 32 -- (envelope-from {tobias.starborg-at-manchester.ac.uk} ) 13, 32 -- id 1Qr4DJ-000AqK-Kt 13, 32 -- for Microscopy-at-microscopy.com; Wed, 10 Aug 2011 09:32:33 +0100 13, 32 -- Received: from MBXP05.ds.man.ac.uk ([169.254.1.2]) by CASP10.ds.man.ac.uk 13, 32 -- ([10.2.172.12]) with mapi id 14.01.0289.008; Wed, 10 Aug 2011 09:32:33 +0100 13, 32 -- From: Tobias Starborg {tobias.starborg-at-manchester.ac.uk} 13, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 32 -- Subject: RE: [Microscopy] Re: Artifacts of the scintillator on a Gatan camera 13, 32 -- Thread-Topic: [Microscopy] Re: Artifacts of the scintillator on a Gatan 13, 32 -- camera 13, 32 -- Thread-Index: AQHMVtAcqP91NZRBSU2PWSZo561pxZUVwXC1 13, 32 -- Date: Wed, 10 Aug 2011 08:28:50 +0000 13, 32 -- Message-ID: {72B0FA1F4D0BCE43B32E5CAD932BBAC50660F5-at-MBXP05.ds.man.ac.uk} 13, 32 -- References: {201108092008.p79K8Spo001710-at-ns.microscopy.com} 13, 32 -- In-Reply-To: {201108092008.p79K8Spo001710-at-ns.microscopy.com} 13, 32 -- Content-Language: en-GB 13, 32 -- X-MS-Has-Attach: 13, 32 -- X-MS-TNEF-Correlator: 13, 32 -- x-originating-ip: [10.2.172.53] 13, 32 -- Content-Type: text/plain; charset="us-ascii" 13, 32 -- MIME-Version: 1.0 13, 32 -- X-Spam-Score: -5.0(?) 13, 32 -- X-Spam-Flag: NO 13, 32 -- Content-Transfer-Encoding: 8bit 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7A8WYFf017796 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both adrian.vega-at-utoronto,ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: adrian.vega-at-utoronto,ca Name: Adrian Vega
Organization: Chemical Engineering - University of Toronto
Title-Subject: [Filtered] EDX on nanoporous material
Message: Good afternnon
I am doing some SEM/EDX analysis on nanoporous alloys containing Ag-Au-Pt with a pore size between 3-15nm. This nanoporous alloy is on top of an alloy with the same elements. The average thickness of this nanoporous layer is around 8um. Typically, I prepare metallographic specimens to either determine the thickness of my nanoporous alloys or to analyze its composition by doing EDX on a cross section of the sample. However, I found that because the Pt concentration is very low (~1at.%) I have to use accelerating voltages higher than 20kV (I have tried lower accelerating voltages obtaining very weir EDX results). I have two main concerns. One is about the effect of porosity in my results because I know that this type of analysis assumes 100% density whether you are using standardless routines or with standards. My second concern, which is related to the first one, is if there is any real concern of having an interference of the substrate in my EDX reading, mainly due to high interaction volume and high porosity. Therefore, I was wondering of there is any correction or consideration to take into account to do this kind of analysis.
Thanks in advance for any help
Best regards
Adrian Vega Login Host: 128.100.199.138 ---------------------------------------------------------------------------
==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 10 07:26:58 2011 11, 28 -- Received: from mx0b-000f2801.pphosted.com (mx0b-000f2801.pphosted.com [67.231.152.119]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ACQuAh005054 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 07:26:57 -0500 11, 28 -- Received: from pps.filterd (m0000739 [127.0.0.1]) 11, 28 -- by mx0b-000f2801.pphosted.com (8.14.3/8.14.3) with SMTP id p7ACQtU6018633 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 08:26:55 -0400 11, 28 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) 11, 28 -- by mx0b-000f2801.pphosted.com with ESMTP id y31bru48a-1 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 08:26:54 -0400 11, 28 -- Received: from NestorPwrBk.local ([192.168.13.114]) 11, 28 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p7ACQs5L001961 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 12:26:54 GMT 11, 28 -- Message-ID: {4E4278EA.1030301-at-microscopy.com} 11, 28 -- Date: Wed, 10 Aug 2011 07:26:18 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: adrian.vega-at-utoronto, ca-at-ns.microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 28 -- MIME-Version: 1.0 11, 28 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:EDX on nanoporous material 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit 11, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 11, 28 -- definitions=2011-08-10_05:2011-08-10,2011-08-10,1970-01-01 signatures=0 11, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 11, 28 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 11, 28 -- engine=6.0.2-1012030000 definitions=main-1108100106 ==============================End of - Headers==============================
There are three artifacts on that image, one near the upper left corner, one at the lower left corner, and one near the lower right corner, respectively.
Regards,
Hongwen Zhou
On Wed, Aug 10, 2011 at 4:43 AM, {tobias.starborg-at-manchester.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Zhou, } } It is difficult to picture what you mean by "artefacts" without an image. Would it be possible for you to host the image on a website and put a link here, so that we can see what you are talking about? I have to admit that I was also one of the people thinking that you were worried by the honeycomb effect of the fibre optics. } } TobyS } } } Dr Tobias Starborg } Senior Experimental Officer: 3DEM } Michael Smith Building } Oxford Road } Manchester } M13 9PT } Tel: +44(0)1612755170 } http://manchesterpolara.org.uk } } ________________________________________ } X-from: zhouhw33-at-gmail.com [zhouhw33-at-gmail.com] } Sent: 09 August 2011 21:08 } To: Tobias Starborg } Subject: [Microscopy] Re: Artifacts of the scintillator on a Gatan camera } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It seems that my words had caused some confusion. To clarify: when I } say "artifacts", I did not refer to the honeycomb pattern. The } artifacts are superimposed on the honeycomb pattern. } } } } On Tue, Aug 9, 2011 at 2:44 PM, Hongwen Zhou {zhouhw33-at-gmail.com} wrote: } } Dear Listers, } } } } We have a Gatan camera showing some unusall features on the gain } } reference images (those "honeycomb" images). These features, according } } to Gatan, are perhaps "physical artifacts" on the phosphor coating. } } They also claim that the scintillator cannot be made defect-free. As } } long as those artifacts do not affect image quality, there is no need } } to replace the scintillator - as the new one may have its own problem. } } } } Has anyone met a similar problem? What would be the long-term effects } } of the physical artifacts (of the phosphor coating) on the performance } } of the camera? Right now, these artifacts can be corrected by software } } method (gain reference corrected). However, will they lead to dead } } pixels eventually? } } } } I should mention that these artifacts are not oil contaminants. We } } baked the camera several times and they are still there. They are not } } hardened oil contaminants either. A service engineer from Gatan had } } cleaned the surface with ethanol. But those features remain on the } } uncorrected images. Their latest explanation is they might be the } } "physical artifacts on the phosphor coating". } } } } Any input is welcome. Also, if you would like to see how those } } artifacts look like, please send me an email. } } } } Kind regards, } } } } Hongwen Zhou } } } } } } Temple University } } 1901 N 13th Street } } Philadelphia, PA 19122 } } Tel: (215) 204-0366 } } } } ==============================Original Headers============================== } 4, 26 -- From zhouhw33-at-gmail.com Tue Aug 9 15:01:43 2011 } 4, 26 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) } 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p79K1h0G020998 } 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Aug 2011 15:01:43 -0500 } 4, 26 -- Received: by iym1 with SMTP id 1so83411iym.0 } 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 09 Aug 2011 13:01:42 -0700 (PDT) } 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 26 -- d=gmail.com; s=gamma; } 4, 26 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to } 4, 26 -- :content-type; } 4, 26 -- bh=ZzBQoYx23CWnoxkV++iqGtAkVt8yxMmiZkuM0Hii+jI=; } 4, 26 -- b=XOu7M1fd8Ld/rgbHIklgOmDr3nc5QxnG1W3ZIC74fVSwyWtrxQc5i0KsPmN344suag } 4, 26 -- 3A7o/34jagFXVgAtRgQBzQCkeczts+aUJcuzBsWoncDbyezRFrV5csM2szbwqBCPL5dg } 4, 26 -- NtkQxHNAyv2Yfvz4fjD2IL/p3hFCDvW648fwU= } 4, 26 -- MIME-Version: 1.0 } 4, 26 -- Received: by 10.42.149.69 with SMTP id u5mr790154icv.38.1312920102529; Tue, 09 } 4, 26 -- Aug 2011 13:01:42 -0700 (PDT) } 4, 26 -- Received: by 10.231.19.204 with HTTP; Tue, 9 Aug 2011 13:01:42 -0700 (PDT) } 4, 26 -- In-Reply-To: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} } 4, 26 -- References: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} } 4, 26 -- Date: Tue, 9 Aug 2011 16:01:42 -0400 } 4, 26 -- Message-ID: {CAODik+v-bVBfHSKtXzO=dVs9X8WTsmhc80O3PwsADgzpOmhr7A-at-mail.gmail.com} } 4, 26 -- Subject: Re: Artifacts of the scintillator on a Gatan camera } 4, 26 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} } 4, 26 -- To: Microscopy-at-microscopy.com } 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 13, 32 -- From tobias.starborg-at-manchester.ac.uk Wed Aug 10 03:32:34 2011 } 13, 32 -- Received: from tranquility.mcc.ac.uk (tranquility.mcc.ac.uk [130.88.200.145]) } 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7A8WYFf017796 } 13, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 03:32:34 -0500 } 13, 32 -- Received: from casp10.ds.man.ac.uk ([10.2.172.12]) } 13, 32 -- by tranquility.mcc.ac.uk with esmtps (TLSv1:AES128-SHA:128) } 13, 32 -- (Exim 4.76 (FreeBSD)) } 13, 32 -- (envelope-from {tobias.starborg-at-manchester.ac.uk} ) } 13, 32 -- id 1Qr4DJ-000AqK-Kt } 13, 32 -- for Microscopy-at-microscopy.com; Wed, 10 Aug 2011 09:32:33 +0100 } 13, 32 -- Received: from MBXP05.ds.man.ac.uk ([169.254.1.2]) by CASP10.ds.man.ac.uk } 13, 32 -- ([10.2.172.12]) with mapi id 14.01.0289.008; Wed, 10 Aug 2011 09:32:33 +0100 } 13, 32 -- From: Tobias Starborg {tobias.starborg-at-manchester.ac.uk} } 13, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 13, 32 -- Subject: RE: [Microscopy] Re: Artifacts of the scintillator on a Gatan camera } 13, 32 -- Thread-Topic: [Microscopy] Re: Artifacts of the scintillator on a Gatan } 13, 32 -- camera } 13, 32 -- Thread-Index: AQHMVtAcqP91NZRBSU2PWSZo561pxZUVwXC1 } 13, 32 -- Date: Wed, 10 Aug 2011 08:28:50 +0000 } 13, 32 -- Message-ID: {72B0FA1F4D0BCE43B32E5CAD932BBAC50660F5-at-MBXP05.ds.man.ac.uk} } 13, 32 -- References: {201108092008.p79K8Spo001710-at-ns.microscopy.com} } 13, 32 -- In-Reply-To: {201108092008.p79K8Spo001710-at-ns.microscopy.com} } 13, 32 -- Content-Language: en-GB } 13, 32 -- X-MS-Has-Attach: } 13, 32 -- X-MS-TNEF-Correlator: } 13, 32 -- x-originating-ip: [10.2.172.53] } 13, 32 -- Content-Type: text/plain; charset="us-ascii" } 13, 32 -- MIME-Version: 1.0 } 13, 32 -- X-Spam-Score: -5.0(?) } 13, 32 -- X-Spam-Flag: NO } 13, 32 -- Content-Transfer-Encoding: 8bit } 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7A8WYFf017796 } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 28 -- From zhouhw33-at-gmail.com Wed Aug 10 07:30:31 2011 10, 28 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ACUT7u007346 10, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 07:30:30 -0500 10, 28 -- Received: by iym1 with SMTP id 1so1164303iym.0 10, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 05:30:29 -0700 (PDT) 10, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 28 -- d=gmail.com; s=gamma; 10, 28 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 10, 28 -- :content-type:content-transfer-encoding; 10, 28 -- bh=I6hbwa3UAGvYN4RQk3QI21benVz+ToXrTZEDbVhtAMs=; 10, 28 -- b=aJ6eamrfz+cOi0TNRnbhNP62XOUDkiKWOk3NI+CYhlFQG/O01sA/klk3QOY/4nf89i 10, 28 -- 22e/BvNOm/SQkOShOij9hvx0oBJT7AUTeS3OUj2HTydTgBG+14fn61dq0S4az9AFnNg6 10, 28 -- gxNQeJQE9potsXFIMf8jGxlxwue7nbbsw5ujs= 10, 28 -- MIME-Version: 1.0 10, 28 -- Received: by 10.231.12.132 with SMTP id x4mr9918852ibx.36.1312979428988; Wed, 10, 28 -- 10 Aug 2011 05:30:28 -0700 (PDT) 10, 28 -- Received: by 10.231.19.204 with HTTP; Wed, 10 Aug 2011 05:29:58 -0700 (PDT) 10, 28 -- In-Reply-To: {201108100843.p7A8hmJX000590-at-ns.microscopy.com} 10, 28 -- References: {201108100843.p7A8hmJX000590-at-ns.microscopy.com} 10, 28 -- Date: Wed, 10 Aug 2011 08:29:58 -0400 10, 28 -- Message-ID: {CAODik+snfN2GDejYa-cPtQjDx0LZXxUVPLXS24FyODUtuRpV-Q-at-mail.gmail.com} 10, 28 -- Subject: Re: [Microscopy] Artifacts of the scintillator on a Gatan camera 10, 28 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7ACUT7u007346 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Shannon.cutt-at-pearson.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Pearson Higher Education / Benjamin Cummings
Title-Subject: [Filtered] Need Electron Micrograph Images
Message: To Whom It May Concern:
My name is Shannon Cutt and IÂ’m a project editor in the anatomy & physiology division of Pearson-Benjamin Cummings publishers. Our team is in desperate need of a few electron micrograph photos. Please write to me if you have the equipment to take EM photos and if you are interested in possibly creating some EM photos for use in an anatomy & physiology textbook? I look forward to hearing from you at your earliest convenience. Thank you very much!
Sincerely,
Shannon Cutt Associate Project Editor
Arts & Sciences Higher Education 1301 Sansome St. San Francisco, CA 94111 T: 415-402-2411 M: 925-858-8223 F: 415-402-2527
Pearson Always Learning Learn more at www.pearson.com
There are three artifacts on that image, one near the upper left corner, one at the lower left corner, and one near the lower right corner, respectively.
Regards,
Hongwen Zhou
On Wed, Aug 10, 2011 at 4:43 AM, {tobias.starborg-at-manchester.ac.uk} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Zhou, } } It is difficult to picture what you mean by "artefacts" without an image. Would it be possible for you to host the image on a website and put a link here, so that we can see what you are talking about? I have to admit that I was also one of the people thinking that you were worried by the honeycomb effect of the fibre optics. } } TobyS } } } Dr Tobias Starborg } Senior Experimental Officer: 3DEM } Michael Smith Building } Oxford Road } Manchester } M13 9PT } Tel: +44(0)1612755170 } http://manchesterpolara.org.uk } } ________________________________________ } X-from: zhouhw33-at-gmail.com [zhouhw33-at-gmail.com] } Sent: 09 August 2011 21:08 } To: Tobias Starborg } Subject: [Microscopy] Re: Artifacts of the scintillator on a Gatan camera } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It seems that my words had caused some confusion. To clarify: when I } say "artifacts", I did not refer to the honeycomb pattern. The } artifacts are superimposed on the honeycomb pattern. } } } } On Tue, Aug 9, 2011 at 2:44 PM, Hongwen Zhou {zhouhw33-at-gmail.com} wrote: } } Dear Listers, } } } } We have a Gatan camera showing some unusall features on the gain } } reference images (those "honeycomb" images). These features, according } } to Gatan, are perhaps "physical artifacts" on the phosphor coating. } } They also claim that the scintillator cannot be made defect-free. As } } long as those artifacts do not affect image quality, there is no need } } to replace the scintillator - as the new one may have its own problem. } } } } Has anyone met a similar problem? What would be the long-term effects } } of the physical artifacts (of the phosphor coating) on the performance } } of the camera? Right now, these artifacts can be corrected by software } } method (gain reference corrected). However, will they lead to dead } } pixels eventually? } } } } I should mention that these artifacts are not oil contaminants. We } } baked the camera several times and they are still there. They are not } } hardened oil contaminants either. A service engineer from Gatan had } } cleaned the surface with ethanol. But those features remain on the } } uncorrected images. Their latest explanation is they might be the } } "physical artifacts on the phosphor coating". } } } } Any input is welcome. Also, if you would like to see how those } } artifacts look like, please send me an email. } } } } Kind regards, } } } } Hongwen Zhou } } } } } } Temple University } } 1901 N 13th Street } } Philadelphia, PA 19122 } } Tel: (215) 204-0366 } } } } ==============================Original Headers============================== } 4, 26 -- From zhouhw33-at-gmail.com Tue Aug 9 15:01:43 2011 } 4, 26 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) } 4, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p79K1h0G020998 } 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Aug 2011 15:01:43 -0500 } 4, 26 -- Received: by iym1 with SMTP id 1so83411iym.0 } 4, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 09 Aug 2011 13:01:42 -0700 (PDT) } 4, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 26 -- d=gmail.com; s=gamma; } 4, 26 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to } 4, 26 -- :content-type; } 4, 26 -- bh=ZzBQoYx23CWnoxkV++iqGtAkVt8yxMmiZkuM0Hii+jI=; } 4, 26 -- b=XOu7M1fd8Ld/rgbHIklgOmDr3nc5QxnG1W3ZIC74fVSwyWtrxQc5i0KsPmN344suag } 4, 26 -- 3A7o/34jagFXVgAtRgQBzQCkeczts+aUJcuzBsWoncDbyezRFrV5csM2szbwqBCPL5dg } 4, 26 -- NtkQxHNAyv2Yfvz4fjD2IL/p3hFCDvW648fwU= } 4, 26 -- MIME-Version: 1.0 } 4, 26 -- Received: by 10.42.149.69 with SMTP id u5mr790154icv.38.1312920102529; Tue, 09 } 4, 26 -- Aug 2011 13:01:42 -0700 (PDT) } 4, 26 -- Received: by 10.231.19.204 with HTTP; Tue, 9 Aug 2011 13:01:42 -0700 (PDT) } 4, 26 -- In-Reply-To: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} } 4, 26 -- References: {CAODik+tDFdxyw-s4APK+Qxao27jhMUuDabOV=oGd0UTvKhL01A-at-mail.gmail.com} } 4, 26 -- Date: Tue, 9 Aug 2011 16:01:42 -0400 } 4, 26 -- Message-ID: {CAODik+v-bVBfHSKtXzO=dVs9X8WTsmhc80O3PwsADgzpOmhr7A-at-mail.gmail.com} } 4, 26 -- Subject: Re: Artifacts of the scintillator on a Gatan camera } 4, 26 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} } 4, 26 -- To: Microscopy-at-microscopy.com } 4, 26 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 13, 32 -- From tobias.starborg-at-manchester.ac.uk Wed Aug 10 03:32:34 2011 } 13, 32 -- Received: from tranquility.mcc.ac.uk (tranquility.mcc.ac.uk [130.88.200.145]) } 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7A8WYFf017796 } 13, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 03:32:34 -0500 } 13, 32 -- Received: from casp10.ds.man.ac.uk ([10.2.172.12]) } 13, 32 -- by tranquility.mcc.ac.uk with esmtps (TLSv1:AES128-SHA:128) } 13, 32 -- (Exim 4.76 (FreeBSD)) } 13, 32 -- (envelope-from {tobias.starborg-at-manchester.ac.uk} ) } 13, 32 -- id 1Qr4DJ-000AqK-Kt } 13, 32 -- for Microscopy-at-microscopy.com; Wed, 10 Aug 2011 09:32:33 +0100 } 13, 32 -- Received: from MBXP05.ds.man.ac.uk ([169.254.1.2]) by CASP10.ds.man.ac.uk } 13, 32 -- ([10.2.172.12]) with mapi id 14.01.0289.008; Wed, 10 Aug 2011 09:32:33 +0100 } 13, 32 -- From: Tobias Starborg {tobias.starborg-at-manchester.ac.uk} } 13, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 13, 32 -- Subject: RE: [Microscopy] Re: Artifacts of the scintillator on a Gatan camera } 13, 32 -- Thread-Topic: [Microscopy] Re: Artifacts of the scintillator on a Gatan } 13, 32 -- camera } 13, 32 -- Thread-Index: AQHMVtAcqP91NZRBSU2PWSZo561pxZUVwXC1 } 13, 32 -- Date: Wed, 10 Aug 2011 08:28:50 +0000 } 13, 32 -- Message-ID: {72B0FA1F4D0BCE43B32E5CAD932BBAC50660F5-at-MBXP05.ds.man.ac.uk} } 13, 32 -- References: {201108092008.p79K8Spo001710-at-ns.microscopy.com} } 13, 32 -- In-Reply-To: {201108092008.p79K8Spo001710-at-ns.microscopy.com} } 13, 32 -- Content-Language: en-GB } 13, 32 -- X-MS-Has-Attach: } 13, 32 -- X-MS-TNEF-Correlator: } 13, 32 -- x-originating-ip: [10.2.172.53] } 13, 32 -- Content-Type: text/plain; charset="us-ascii" } 13, 32 -- MIME-Version: 1.0 } 13, 32 -- X-Spam-Score: -5.0(?) } 13, 32 -- X-Spam-Flag: NO } 13, 32 -- Content-Transfer-Encoding: 8bit } 13, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7A8WYFf017796 } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 28 -- From zhouhw33-at-gmail.com Wed Aug 10 07:34:53 2011 10, 28 -- Received: from mail-yi0-f41.google.com (mail-yi0-f41.google.com [209.85.218.41]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ACYqpT019953 10, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 07:34:53 -0500 10, 28 -- Received: by yib2 with SMTP id 2so691933yib.0 10, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 05:34:50 -0700 (PDT) 10, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 28 -- d=gmail.com; s=gamma; 10, 28 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 10, 28 -- :content-type:content-transfer-encoding; 10, 28 -- bh=I6hbwa3UAGvYN4RQk3QI21benVz+ToXrTZEDbVhtAMs=; 10, 28 -- b=aJ6eamrfz+cOi0TNRnbhNP62XOUDkiKWOk3NI+CYhlFQG/O01sA/klk3QOY/4nf89i 10, 28 -- 22e/BvNOm/SQkOShOij9hvx0oBJT7AUTeS3OUj2HTydTgBG+14fn61dq0S4az9AFnNg6 10, 28 -- gxNQeJQE9potsXFIMf8jGxlxwue7nbbsw5ujs= 10, 28 -- MIME-Version: 1.0 10, 28 -- Received: by 10.42.158.134 with SMTP id h6mr8427993icx.172.1312979398995; Wed, 10, 28 -- 10 Aug 2011 05:29:58 -0700 (PDT) 10, 28 -- Received: by 10.231.19.204 with HTTP; Wed, 10 Aug 2011 05:29:58 -0700 (PDT) 10, 28 -- In-Reply-To: {201108100843.p7A8hmJX000590-at-ns.microscopy.com} 10, 28 -- References: {201108100843.p7A8hmJX000590-at-ns.microscopy.com} 10, 28 -- Date: Wed, 10 Aug 2011 08:29:58 -0400 10, 28 -- Message-ID: {CAODik+snfN2GDejYa-cPtQjDx0LZXxUVPLXS24FyODUtuRpV-Q-at-mail.gmail.com} 10, 28 -- Subject: Re: [Microscopy] Artifacts of the scintillator on a Gatan camera 10, 28 -- From: Hongwen Zhou {zhouhw33-at-gmail.com} 10, 28 -- To: Microscopy-at-microscopy.com 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7ACYqpT019953 ==============================End of - Headers==============================
This looks completely normal to me- in fact it looks pretty clean. It seems like the image is inverted, compared to how our US1000 shows the gain ref- i.e. the gaps between the bundles of fibres should be dark, not bright.
The line in the bottom right looks like a ripple in the scintillator- our older side-mount camera has many more of these- looks a bit like a rippled sheet that has been pulled from one point on the edge, and it does not affect the images once gain reference correction has been applied.
The two lighter patches on the left side I think are contamination (would be dark patches if the image was the not inverted), but as they are not completely opaque (as well as being on the edge of the frame which makes the less noticeable) I don't think they will have any effect on your images. Even small completely opaque contamination can be removed quite well by the Gatan software. Over a certain number of pixels being obscured, it can be a problem- they turn up as black hard-edges spots on all images in the same place.
Ben
-- Research Support Manager MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271866 {http://mrcanu.pharm.ox.ac.uk/}
On 10/08/2011 13:45, "zhouhw33-at-gmail.com" {zhouhw33-at-gmail.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 15, 31 -- From ben.micklem-at-pharm.ox.ac.uk Wed Aug 10 08:44:50 2011 15, 31 -- Received: from relay0.mail.ox.ac.uk (relay0.mail.ox.ac.uk [129.67.1.161]) 15, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ADinNr006114 15, 31 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 08:44:50 -0500 15, 31 -- Received: from smtp4.nexus.ox.ac.uk ([163.1.154.138] helo=exht04.ad.oak.ox.ac.uk) 15, 31 -- by relay0.mail.ox.ac.uk with esmtp (Exim 4.75) 15, 31 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 15, 31 -- id 1Qr95U-0007q5-1D 15, 31 -- for microscopy-at-microscopy.com; Wed, 10 Aug 2011 14:44:48 +0100 15, 31 -- Received: from EXMBX04.ad.oak.ox.ac.uk ([169.254.2.205]) by 15, 31 -- exht04.ad.oak.ox.ac.uk ([163.1.154.55]) with mapi; Wed, 10 Aug 2011 14:44:48 15, 31 -- +0100 15, 31 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 15, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 15, 31 -- Date: Wed, 10 Aug 2011 14:44:47 +0100 15, 31 -- Subject: Re: [Microscopy] Re: Artifacts of the scintillator on a Gatan camera 15, 31 -- Thread-Topic: [Microscopy] Re: Artifacts of the scintillator on a Gatan 15, 31 -- camera 15, 31 -- Thread-Index: AcxXY6wACNnGhJcARbezoJzHe1jWRQ== 15, 31 -- Message-ID: {CA6844EB.14F7B%ben.micklem-at-pharm.ox.ac.uk} 15, 31 -- In-Reply-To: {201108101245.p7ACjDa5015174-at-ns.microscopy.com} 15, 31 -- Accept-Language: en-US, en-GB 15, 31 -- Content-Language: en-US 15, 31 -- X-MS-Has-Attach: 15, 31 -- X-MS-TNEF-Correlator: 15, 31 -- user-agent: Microsoft-MacOutlook/14.12.0.110505 15, 31 -- acceptlanguage: en-US, en-GB 15, 31 -- Content-Type: text/plain; charset="us-ascii" 15, 31 -- MIME-Version: 1.0 15, 31 -- Content-Transfer-Encoding: 8bit 15, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7ADinNr006114 ==============================End of - Headers==============================
I am with Dalhousie University MEMS research group and my research area is focused on electrothermal actuators. I am looking for a method to apply and control electrical power (Voltage=5~10 V, Current=2~10 mA) to MEMS chips while it is in SEM or FIB vacuum chamber. Please let me know if you are aware of any technique or tools/kits that can be used to achieve this goal. SEM model: “Hitachi S-4700, FE-SEM”. FIB model: "Hitachi FB-2000A"
Kind regards,
Mehrdad
==============================Original Headers============================== 5, 22 -- From mh281909-at-dal.ca Wed Aug 10 09:19:06 2011 5, 22 -- Received: from sxt-sm-1.ucis.dal.ca (SXT-SM-1.UCIS.Dal.Ca [129.173.5.72]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7AEJ5CR022899 5, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 09:19:05 -0500 5, 22 -- Received: from localhost (kil-upt-12.ITS.Dal.Ca [129.173.21.7]) 5, 22 -- by sxt-sm-1.ucis.dal.ca (8.14.2+Sun/8.14.2) with ESMTP id p7AEHmAL008683 5, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 11:17:48 -0300 (ADT) 5, 22 -- Received: from Jonathan.me.Dal.Ca (Jonathan.me.Dal.Ca [129.173.86.83]) by 5, 22 -- wm2.dal.ca (Horde Framework) with HTTP; Wed, 10 Aug 2011 11:17:48 -0300 5, 22 -- Message-ID: {20110810111748.19022boz8l5vhgys-at-wm2.dal.ca} 5, 22 -- Date: Wed, 10 Aug 2011 11:17:48 -0300 5, 22 -- From: Mehrdad Mottaghi {mh281909-at-dal.ca} 5, 22 -- To: Microscopy-at-microscopy.com 5, 22 -- Subject: Applying voltage to MEMS chip in SEM/FIB 5, 22 -- MIME-Version: 1.0 5, 22 -- Content-Type: text/plain; 5, 22 -- charset=ISO-8859-1; 5, 22 -- DelSp="Yes"; 5, 22 -- format="flowed" 5, 22 -- Content-Disposition: inline 5, 22 -- Content-Transfer-Encoding: 8bit 5, 22 -- User-Agent: Internet Messaging Program (IMP) H3 (4.3.4) ==============================End of - Headers==============================
Given those elements, you may be okay using higher voltages. You probably want to use something like Casino or some other Monte Carlo method to estimate the excitation volume. I ran simulations on pure Ag (the lightest component) and found that the excitation depth was about 1 um at 30 kV. It was about 700 nm at 25k V and about 500 nm at 20 kV. You should not be exciting your substrate.
I am uncertain what you mean when you say you had problems with low Pt concentration calling for higher accelerating voltages. What is your Au content? If it is high, then you need to make sure that your system is deconvoluting well. It will be more challenging to pick out a small intensity for the Pt M line next to a strong intensity for the Au line; however, it should be possible. You could use the L lines around 9.5 keV, but the intensity is much lower than for the M lines.
Such challenging deconvolutions will need critical review rather than simply believing the numbers. You should check the fit and examine the residuals to make sure everything is accounted for. I recommend that for all critical applications.
If there is a problem with your energy calibration and the peaks shift, then you could really have problems with the Pt intensity. Residuals would show leftover intensity on one side of the peak and a deficit of intensity on the other. Of course, that could be reflected in errors in the Pt concentration.
You could also have problems if the stored peak widths are not the same as the operating conditions at the time of analysis. We have an old Oxford ISIS system. The factory peak profiles were collected at the highest resolution setting, but we usually reduce the pulse processing time to count faster. Our peaks are a bit broader and the default profiles don't fit the actual spectra very well. (New software probably takes the pulse processing time into account and adjusts the profiles.) Therefore, we have collected our own profiles under the exact same conditions. The default profiles are pretty good, but our own profiles take all possible differences between our system and the factory into account.
I would recommend comparing results for L lines and M lines. At 30 kV, they should give you similar numbers if the deconvolution is good.
I would also recommend testing the analyses on a sample with no Pt (maybe on a sample of pure gold). You could overly the two spectra to see if Pt appears on a shoulder of the Au peak. You could also check the deconvolution results. If the software still reports Pt in the no-Pt sample, then you should downgrade the trust in your software.
Offhand, I don't know that porosity of 3-15 nm should cause problems with the analysis, provided it is homogeneously distributed. It would allow a deeper penetration of the beam, but it would allow a longer path-length for absorption on the way out. The mass absorption effects on the way in and the way out should be the same.
EDS can be quite a powerful technique if done well with good software. Just beware that not all software is created equal. You will need to watch it carefully as you apply it to a number of problems and determine how much trust you can put into the results.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, August 10, 2011 7:29 AM To: wesaia-at-iastate.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both adrian.vega-at-utoronto,ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: adrian.vega-at-utoronto,ca Name: Adrian Vega
Organization: Chemical Engineering - University of Toronto
Title-Subject: [Filtered] EDX on nanoporous material
Message: Good afternnon
I am doing some SEM/EDX analysis on nanoporous alloys containing Ag-Au-Pt with a pore size between 3-15nm. This nanoporous alloy is on top of an alloy with the same elements. The average thickness of this nanoporous layer is around 8um. Typically, I prepare metallographic specimens to either determine the thickness of my nanoporous alloys or to analyze its composition by doing EDX on a cross section of the sample. However, I found that because the Pt concentration is very low (~1at.%) I have to use accelerating voltages higher than 20kV (I have tried lower accelerating voltages obtaining very weir EDX results). I have two main concerns. One is about the effect of porosity in my results because I know that this type of analysis assumes 100% density whether you are using standardless routines or with standards. My second concern, which is related to the first one, is if there is any real concern of having an interference of the substrate in my EDX reading, mainly due to high interaction volume and high porosity. Therefore, I was wondering of there is any correction or consideration to take into account to do this kind of analysis.
Thanks in advance for any help
Best regards
Adrian Vega Login Host: 128.100.199.138 ---------------------------------------------------------------------------
==============================Original Headers============================== 11, 28 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 10 07:26:58 2011 11, 28 -- Received: from mx0b-000f2801.pphosted.com (mx0b-000f2801.pphosted.com [67.231.152.119]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ACQuAh005054 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 07:26:57 -0500 11, 28 -- Received: from pps.filterd (m0000739 [127.0.0.1]) 11, 28 -- by mx0b-000f2801.pphosted.com (8.14.3/8.14.3) with SMTP id p7ACQtU6018633 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 08:26:55 -0400 11, 28 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) 11, 28 -- by mx0b-000f2801.pphosted.com with ESMTP id y31bru48a-1 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 08:26:54 -0400 11, 28 -- Received: from NestorPwrBk.local ([192.168.13.114]) 11, 28 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p7ACQs5L001961 11, 28 -- for {microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 12:26:54 GMT 11, 28 -- Message-ID: {4E4278EA.1030301-at-microscopy.com} 11, 28 -- Date: Wed, 10 Aug 2011 07:26:18 -0500 11, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 28 -- Reply-To: adrian.vega-at-utoronto, ca-at-ns.microscopy.com 11, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 28 -- MIME-Version: 1.0 11, 28 -- To: microscopyListServer-Forward {microscopy-at-microscopy.com} 11, 28 -- Subject: viaWWW:EDX on nanoporous material 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 28 -- Content-Transfer-Encoding: 7bit 11, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 11, 28 -- definitions=2011-08-10_05:2011-08-10,2011-08-10,1970-01-01 signatures=0 11, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 11, 28 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 11, 28 -- engine=6.0.2-1012030000 definitions=main-1108100106 ==============================End of - Headers==============================
==============================Original Headers============================== 28, 32 -- From wesaia-at-iastate.edu Wed Aug 10 10:04:21 2011 28, 32 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) 28, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7AF4Jx1007452 28, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Aug 2011 10:04:20 -0500 28, 32 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702a.its.iastate.edu [10.10.87.140]) 28, 32 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id p7AF4HG2019704; 28, 32 -- Wed, 10 Aug 2011 10:04:17 -0500 28, 32 -- Received: from EXITS711.its.iastate.edu ([10.10.87.143]) by 28, 32 -- EXITS702A.its.iastate.edu ([10.10.87.140]) with mapi; Wed, 10 Aug 2011 28, 32 -- 10:04:11 -0500 28, 32 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 28, 32 -- To: "adrian.vega-at-utoronto.ca" {adrian.vega-at-utoronto.ca} 28, 32 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 28, 32 -- Date: Wed, 10 Aug 2011 10:04:16 -0500 28, 32 -- Subject: RE: [Microscopy] viaWWW:EDX on nanoporous material 28, 32 -- Thread-Topic: [Microscopy] viaWWW:EDX on nanoporous material 28, 32 -- Thread-Index: AcxXWRbYlgRoKtirR5aWVCSoc9PnawAECsGA 28, 32 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F14681CF412-at-EXITS711.its.iastate.edu} 28, 32 -- References: {201108101229.p7ACT0vf005963-at-ns.microscopy.com} 28, 32 -- In-Reply-To: {201108101229.p7ACT0vf005963-at-ns.microscopy.com} 28, 32 -- Accept-Language: en-US 28, 32 -- Content-Language: en-US 28, 32 -- X-MS-Has-Attach: 28, 32 -- X-MS-TNEF-Correlator: 28, 32 -- acceptlanguage: en-US 28, 32 -- Content-Type: text/plain; charset="us-ascii" 28, 32 -- MIME-Version: 1.0 28, 32 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.8.10.145714 28, 32 -- X-ISUMailhub-test: Gauge=XIIIIIIIII, Probability=19%, Report=' 28, 32 -- RATWARE_RCVD_BONUS_SPC 2.0, SUPERLONG_LINE 0.05, DATE_TZ_NA 0, DATE_TZ_NEG_0500 0, FROM_NAME_PHRASE 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __C230066_P5 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_CONTACT_NAME 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __STOCK_PHRASE_7 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' 28, 32 -- Content-Transfer-Encoding: 8bit 28, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7AF4Jx1007452 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: Ram Tiruvalam
Organization: Haldor Topsøe
Title-Subject: [Filtered] Color SEM images
Message: Hi everyone,
This might seem like a basic question so please bear with me. I am looking for the best way to color SEM images . I am only aware of a few techniques such as (a)acquiring images at diffrent accelerating voltages and using them as RGB channels (b) using matlab scripts such as "Colorization using Optimization" (c) using custom color tables. But none of these techniques give me the stunning images i have seen from various groups. how are the stunning color SEM images generated? Should i use Adobe Photoshop ?
Any ideas? I have access to a regular SEM with one SE detector and one BSE detector.
A simple way is to take 3 separate images with individual quadrants of your BSE detector, then combine them in photoshop or other package, colouring the individual channels red, green and blue, respectively. Or some other colour combination. Someone else will probably know the software package you can buy to do the other type of image colourisation - where individual objects are coloured differently. cheers, Rosemary
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334
On 12/08/11 8:52 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 9, 32 -- From prvs=1975de54a=Rosemary.White-at-csiro.au Thu Aug 11 18:02:33 2011 9, 32 -- Received: from act-ironport-ext.csiro.au (act-mx-ext.csiro.au [150.229.7.36]) 9, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7BN2WNL000590 9, 32 -- for {microscopy-at-microscopy.com} ; Thu, 11 Aug 2011 18:02:33 -0500 9, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 9, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 9, 32 -- s=email; t=1313103753; x=1344639753; 9, 32 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 9, 32 -- content-transfer-encoding; 9, 32 -- bh=4AEfK7oyfBBmewoNEgPIM1LokCgADVtIlkYmnsmmt7c=; 9, 32 -- b=ISlGHesifUthC+gfp7jE2MMnIUUPJDTsKcGkoEzNfete1dvubiVYJ/GU 9, 32 -- R9B5cds9Dr0shOpltT6dSNWovfaZF7iYekPKcODez6P38giDw54/vJB2q 9, 32 -- XwFqBngb5KTwFoB; 9, 32 -- X-IronPort-AV: E=Sophos;i="4.67,358,1309701600"; 9, 32 -- d="scan'208";a="27022582" 9, 32 -- Received: from exvic-htca02.nexus.csiro.au ([138.194.81.127]) 9, 32 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 12 Aug 2011 09:02:31 +1000 9, 32 -- Received: from [152.83.195.117] (152.83.195.117) by 9, 32 -- exvic-htca02.nexus.csiro.au (138.194.81.127) with Microsoft SMTP Server (TLS) 9, 32 -- id 8.3.159.2; Fri, 12 Aug 2011 09:02:31 +1000 9, 32 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 9, 32 -- Date: Fri, 12 Aug 2011 09:02:28 +1000 9, 32 -- Subject: Re: [Microscopy] viaWWW:Color SEM images 9, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} 9, 32 -- To: {microscopy-at-microscopy.com} , {rct204-at-gmail.com} 9, 32 -- Message-ID: {CA6A9BB9.2716F%rosemary.white-at-csiro.au} 9, 32 -- Thread-Topic: [Microscopy] viaWWW:Color SEM images 9, 32 -- In-Reply-To: {201108112252.p7BMq7cw024486-at-ns.microscopy.com} 9, 32 -- MIME-Version: 1.0 9, 32 -- Content-Type: text/plain; charset="ISO-8859-1" 9, 32 -- Content-Transfer-Encoding: 8bit 9, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7BN2WNL000590 ==============================End of - Headers==============================
Do you mean colorized like my images http://www5.pbrc.hawaii.edu/microangela/ or Dennis Kunkel's http://www.denniskunkel.com/ all taken with "my" SEMs at the University of Hawaii? We use Photoshop.
In short, you need to learn to use your selection tools (especially Magic Wand and Lasso) really well, then save and use the selected areas as masks so that you can paint or fill with color in Color Mode.
If this is too brief, let me know.
Aloha, Tina
} This might seem like a basic question so please bear with me. I am } looking for the best way to color SEM images . I am only aware of a few } techniques such as (a)acquiring images at diffrent accelerating voltages } and using them as RGB channels (b) using matlab scripts such as } "Colorization using Optimization" (c) using custom color tables. } But none of these techniques give me the stunning images i have seen } from various groups. how are the stunning color SEM images generated? } Should i use Adobe Photoshop ? } } Any ideas? } I have access to a regular SEM with one SE detector and one BSE detector.
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 7, 22 -- From tina-at-pbrc.hawaii.edu Thu Aug 11 19:14:53 2011 7, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 7, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7C0EqdS017517 7, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Aug 2011 19:14:53 -0500 7, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 7, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p7C0EoGK014069 7, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 7, 22 -- Thu, 11 Aug 2011 14:14:50 -1000 (HST) 7, 22 -- Received: from localhost (tina-at-localhost) 7, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p7C0EmkR014065; 7, 22 -- Thu, 11 Aug 2011 14:14:48 -1000 (HST) 7, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 7, 22 -- Date: Thu, 11 Aug 2011 14:14:48 -1000 (HST) 7, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 7, 22 -- X-X-Sender: tina-at-b1000 7, 22 -- To: rct204-at-gmail.com, Microscopy Listserver {Microscopy-at-microscopy.com} 7, 22 -- Subject: Re: [Microscopy] viaWWW:Color SEM images 7, 22 -- In-Reply-To: {201108112245.p7BMjFCr017901-at-ns.microscopy.com} 7, 22 -- Message-ID: {Pine.GSO.4.64.1108111413070.13951-at-b1000} 7, 22 -- References: {201108112245.p7BMjFCr017901-at-ns.microscopy.com} 7, 22 -- MIME-Version: 1.0 7, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
Hi Ram, as others from the list answered there are two principal ways to get colour in your SEM images: - make good black and white images and add a lot of "Photoshop magic" to bring colour to the specimen - use multiple detectors and perhaps also different kind of detectors (like SE or backscattered...) to get different signals back from the specimen and use these signals to attribute aesthetical colours to the image (and add some Photoshop magic...).
For example: I use the secondary electrons for getting a very fast separation of parts on specimen with a small interaction volume like hairs on insects or cells or substrate etc. The backscattered detectors (I have three) I use to get surfaces (topography) ad hoc separated in a different colour. You can also try to get more different images with different accelerating voltages and the best suited detectors for it... Also distance of the detectors to the specimen can make very different iamges...
There is a PDF from a MIKROKOSMOS article which describes my work, but only in German language: www.elektronenmikroskopie.info/pdf/Mikrokosmos06-99.pdf (1 MB)
I am using a digital image aquisition system from point electronic, www.pointelectronic.de, which can handle up to 8 detectors and scan up to 4 detectors in a resolution of max. 16kx16k pixel. The scanning software has the ability to directly mix the signals from the SEM into a coloured image.
For the images, have a look at: http://www.elektronenmikroskopie.info/galerie%20-%20rem-biologie.htm for biologic specimen and http://www.elektronenmikroskopie.info/galerie%20-%20rem-material.htm for some images from material science. Sorry, pages are only in German language up to now and the site is still in construction.
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 12.08.11 00:48, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both rct204-at-gmail.com as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: rct204-at-gmail.com Name: Ram Tiruvalam } } Organization: Haldor Topsøe } } Title-Subject: [Filtered] Color SEM images } } Message: Hi everyone, } } This might seem like a basic question so please bear with me. I am } looking for the best way to color SEM images . I am only aware of a few } techniques such as (a)acquiring images at diffrent accelerating voltages } and using them as RGB channels (b) using matlab scripts such as } "Colorization using Optimization" (c) using custom color tables. } But none of these techniques give me the stunning images i have seen } from various groups. how are the stunning color SEM images generated? } Should i use Adobe Photoshop ? } } Any ideas? } I have access to a regular SEM with one SE detector and one BSE detector. } } Ram } } } Login Host: 195.41.211.254 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 28 -- From microscopylistserver-noreply-at-microscopy.com Thu Aug 11 17:44:16 2011 } 12, 28 -- Received: from mx0b-000f2801.pphosted.com (mx0b-000f2801.pphosted.com [67.231.152.119]) } 12, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP idp7BMiGlH016651 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 11 Aug 2011 17:44:16 -0500 } 12, 28 -- Received: from pps.filterd (m0000739 [127.0.0.1]) } 12, 28 -- by mx0b-000f2801.pphosted.com (8.14.3/8.14.3) with SMTP id p7BMhAuW030770 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 11 Aug 2011 18:44:16 -0400 } 12, 28 -- Received: from et-bna-5.site.stayonline.net ([12.191.171.2]) } 12, 28 -- by mx0b-000f2801.pphosted.com with ESMTP id y403ehw2w-1 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 11 Aug 2011 18:44:15 -0400 } 12, 28 -- Received: from NestorPwrBk.local ([192.168.13.114]) } 12, 28 -- by et-bna-5.site.stayonline.net (8.13.8/8.12.6) with ESMTP id p7BMiFCM046887 } 12, 28 -- for {microscopy-at-microscopy.com} ; Thu, 11 Aug 2011 22:44:15 GMT } 12, 28 -- Message-ID: {4E445B3E.90803-at-microscopy.com} } 12, 28 -- Date: Thu, 11 Aug 2011 17:44:14 -0500 } 12, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 28 -- Reply-To: rct204-at-gmail.com } 12, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 28 -- MIME-Version: 1.0 } 12, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 28 -- Subject: viaWWW:Color SEM images } 12, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 28 -- Content-Transfer-Encoding: 8bit } 12, 28 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 } 12, 28 -- definitions=2011-08-11_08:2011-08-12,2011-08-11,1970-01-01signatures=0 } 12, 28 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 } 12, 28 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx } 12, 28 -- engine=6.0.2-1012030000 definitions=main-1108110246 } ==============================End of - Headers============================== }
==============================Original Headers============================== 14, 23 -- From stefan.diller-at-t-online.de Fri Aug 12 10:33:41 2011 14, 23 -- Received: from mailout07.t-online.de (mailout07.t-online.de [194.25.134.83]) 14, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7CFXf17028881 14, 23 -- for {microscopy-at-microscopy.com} ; Fri, 12 Aug 2011 10:33:41 -0500 14, 23 -- Received: from fwd09.aul.t-online.de (fwd09.aul.t-online.de ) 14, 23 -- by mailout07.t-online.de with smtp 14, 23 -- id 1Qrtjw-0002sD-6a; Fri, 12 Aug 2011 17:33:40 +0200 14, 23 -- Received: from mac-pro.speedport.ip (TFqFRMZlQhuPnWqqFJ-VBHRjPC2ANqoCvZ1lQ+a7y8dIB6n+-YE7C89sjSfJRBSQmr-at-[87.178.228.65]) by fwd09.t-online.de 14, 23 -- with esmtp id 1Qrtjk-1WkzuC0; Fri, 12 Aug 2011 17:33:28 +0200 14, 23 -- Message-ID: {4E4547C8.1060003-at-t-online.de} 14, 23 -- Date: Fri, 12 Aug 2011 17:33:28 +0200 14, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 14, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:5.0) Gecko/20110624 Thunderbird/5.0 14, 23 -- MIME-Version: 1.0 14, 23 -- To: microscopy-at-microscopy.com, rct204-at-gmail.com 14, 23 -- Subject: Re: [Microscopy] viaWWW:Color SEM images 14, 23 -- References: {201108112248.p7BMmvf1022674-at-ns.microscopy.com} 14, 23 -- In-Reply-To: {201108112248.p7BMmvf1022674-at-ns.microscopy.com} 14, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 23 -- X-ID: TFqFRMZlQhuPnWqqFJ-VBHRjPC2ANqoCvZ1lQ+a7y8dIB6n+-YE7C89sjSfJRBSQmr 14, 23 -- X-TOI-MSGID: aeabee67-dda8-4cb3-abf4-7f235b755d0c 14, 23 -- Content-Transfer-Encoding: 8bit 14, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7CFXf17028881 ==============================End of - Headers==============================
Hmmm, is it just a fancy way of presenting extended focus images? You click on the picture and it knows which image in the through focus series to show?
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 M 61 2 420 972 028 E rosemary.white-at-csiro.au
On 12/08/11 10:19 PM, "jehrman-at-mta.ca" {jehrman-at-mta.ca} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 31 -- From prvs=200ac683c=Rosemary.White-at-csiro.au Sat Aug 13 20:21:35 2011 8, 31 -- Received: from vic-ironport-ext.csiro.au (vic-mx-ext.csiro.au [150.229.64.36]) 8, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7E1LXhc012567 8, 31 -- for {microscopy-at-microscopy.com} ; Sat, 13 Aug 2011 20:21:35 -0500 8, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 8, 31 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 8, 31 -- s=email; t=1313284895; x=1344820895; 8, 31 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 8, 31 -- content-transfer-encoding; 8, 31 -- bh=1Th8lSSuhK9exDBvYIOaGnKDbyAq4UozCRZCwGcEM78=; 8, 31 -- b=q/i8rIBZDtyONFblLTsTrNI1KE86go5hG2HWNoleYQjDu4jS9+h/ZkLy 8, 31 -- s9fzeTC7vEFfbj0xeJj5oE383H/RsIvyptridg2ziPP6SwcRM8Xml/CWm 8, 31 -- E+6jYUtYHatkE/G; 8, 31 -- X-IronPort-AV: E=Sophos;i="4.67,367,1309701600"; 8, 31 -- d="scan'208";a="66179848" 8, 31 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 8, 31 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 14 Aug 2011 11:21:32 +1000 8, 31 -- Received: from [152.83.195.117] (152.83.195.117) by 8, 31 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 8, 31 -- (TLS) id 8.3.159.2; Sun, 14 Aug 2011 11:21:31 +1000 8, 31 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 8, 31 -- Date: Sun, 14 Aug 2011 11:21:27 +1000 8, 31 -- Subject: Re: [Microscopy] Lytro camera 8, 31 -- From: Rosemary White {rosemary.white-at-csiro.au} 8, 31 -- To: {microscopy-at-microscopy.com} 8, 31 -- Message-ID: {CA6D5FAD.2726B%rosemary.white-at-csiro.au} 8, 31 -- Thread-Topic: [Microscopy] Lytro camera 8, 31 -- In-Reply-To: {201108121219.p7CCJBAA016609-at-ns.microscopy.com} 8, 31 -- MIME-Version: 1.0 8, 31 -- Content-Type: text/plain; charset="US-ASCII" 8, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This type of camera is known as a plenoptic camera. Wikipedia has a short description of this technology here:
http://en.wikipedia.org/wiki/Plenoptic_camera
The references do a good job explaining the technology in depth. In fact, reference 2 is a paper coauthored by the founder, Dr. Ren Ng, of Lytro. This video from Adobe shows the technology in action:
http://www.youtube.com/watch?v=jS7usnHmNZ0
Cheers, Chris
On Sat, Aug 13, 2011 at 9:31 PM, {rosemary.white-at-csiro.au} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hmmm, is it just a fancy way of presenting extended focus images? You } click on the picture and it knows which image in the through focus series } to show? } } Rosemary White } CSIRO Plant Industry } GPO Box 1600 } Canberra, ACT 2601 } Australia } } T 61 2 6246 5475 } F 61 2 6246 5334 } M 61 2 420 972 028 } E rosemary.white-at-csiro.au } } } On 12/08/11 10:19 PM, "jehrman-at-mta.ca" {jehrman-at-mta.ca} wrote: } } } } } } } } } -------------------------------------------------------------------------- } } -- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver
-- Christopher Winkler Dynamic Characterization Group http://www.materials.drexel.edu/dcg/ Drexel University 267-496-0587
==============================Original Headers============================== 11, 28 -- From microwink-at-gmail.com Sat Aug 13 21:08:59 2011 11, 28 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 11, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7E28wrx003739 11, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 13 Aug 2011 21:08:59 -0500 11, 28 -- Received: by bkbzt4 with SMTP id zt4so2778045bkb.0 11, 28 -- for {Microscopy-at-microscopy.com} ; Sat, 13 Aug 2011 19:08:58 -0700 (PDT) 11, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 28 -- d=gmail.com; s=gamma; 11, 28 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 11, 28 -- :content-type:content-transfer-encoding; 11, 28 -- bh=BnAJkDc8L0c7IqS0K9TW7NFiNZaCswiM/NFuZxrz1pE=; 11, 28 -- b=E8rDMOwA55twBtGHY6FmrXMtdn6O69WNzu+2iKDZ4GoKR8WgpNIyQxgi1itNXWxFXg 11, 28 -- TKTUgNYMLEEnS38UK4R808cvVOkrvsCM9zySCyg0C8EmrzF1HrB35gyEVurwNOVI//nY 11, 28 -- 2nF8ePTWxn1pf+rsFkiustwGH446r5MkSNN7c= 11, 28 -- MIME-Version: 1.0 11, 28 -- Received: by 10.204.232.131 with SMTP id ju3mr304430bkb.169.1313287736999; 11, 28 -- Sat, 13 Aug 2011 19:08:56 -0700 (PDT) 11, 28 -- Received: by 10.204.24.144 with HTTP; Sat, 13 Aug 2011 19:08:56 -0700 (PDT) 11, 28 -- In-Reply-To: {201108140131.p7E1VPJq024990-at-ns.microscopy.com} 11, 28 -- References: {201108140131.p7E1VPJq024990-at-ns.microscopy.com} 11, 28 -- Date: Sat, 13 Aug 2011 22:08:56 -0400 11, 28 -- Message-ID: {CAA8T2PMNBNxVt8oZkqay=uKwjbdJSp8ovhT=tTYC304+6ovkrQ-at-mail.gmail.com} 11, 28 -- Subject: Re: [Microscopy] Re: Lytro camera 11, 28 -- From: Christopher Winkler {microwink-at-gmail.com} 11, 28 -- To: Microscopy-at-microscopy.com 11, 28 -- Content-Type: text/plain; charset=ISO-8859-1 11, 28 -- Content-Transfer-Encoding: 8bit 11, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7E28wrx003739 ==============================End of - Headers==============================
I saw this (new) technology, and my first impulse was the same: Can that be used for microscopy? If you look at the technology, it works essentially by breaking up the image into many smaller images through the use of an array of microlenses. These lenses are spatially distributed, and each one sees the object from a slightly different angle. It's like "stereo on steroids" (Hmm, perhaps I should trademark that :-). A modern light microscope is "infinity corrected". The objective lenses are designed so that the light is focused to infinity. If I understand that correctly, the microlenses in a such a camera would all see the same image, and the images could not be focused after the fact. Of course, it might be possible to design objective lenses that allow the use of such a camera.
P.S.: I'd like to thank everybody who came to our booth at the M&M meeting in Nashville. I hope everybody who was there had a productive and good time.
--- Mike Bode, Ph.D. ResAlta Research Technologies Corp.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: Ram Chandra
Organization: HALDOR TOPSØE
Title-Subject: [Filtered] Color SEM images- Thanks
Message: Dear All,
Thank you very much for the wonderful responses and teaching me how to color SEM images. I will definitely try some of the techniques and software (Thanks for the Gimp tip Bryan) you have mentioned.
Rosemary: I will definitely try you technique as it seems possible on my microscope. thanks.
Tina: Your images and coloring are spectacular. In the cases of "More stuff" and "bacteria" in the grows on you section (http://www5.pbrc.hawaii.edu/microangela/)it seems spectacularly hard to color each one of the bacteria and fibers individually with the magic lasso tool. Is it just a lot of patience? Or do you have some nice tricks? I am surprised that the borders can look so crisp after photoshop manipulation.
Stefan: Your images just look very natural and extremely beautiful. thanks for sharing it.(http://www.elektronenmikroskopie.info/galerie%20-%20rem-biologie.htm) Let me see how good google translate is with your german paper :-)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jnorona1-at-jhu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jnorona1-at-jhu.edu Name: Joao Norona
Organization: Johns Hopkins University
Title-Subject: [Filtered] Help - Johns Hopkins grad Student research project
Message: My name is Joao Noroña and I’m a graduate student from Johns Hopkins University. I’m doing a project regarding illumination sources in the Microscopy market and would really appreciate it if you could spare couple of minutes to take a brief online survey regarding the types of equipment you use in your labs and their illumination sources.
I came across this list serve during my online research, and hopefully, with your help, I will be able to get some information that is not available online.
IÂ’m not trying to push any product or sell you anything, I promise! IÂ’m just trying to get some data directly from the people that know this type of equipment best, the people on this listserve (experienced users). As you know, the more data points I gather, the more validity there will be to my research, so if after answering the survey you feel comfortable enough, I would greatly appreciate it if you could forward it along to fellow researchers at other labs.
I realize that this request may be a bit intrusive and I apologize in advance, I just have not been able to find this information elsewhere.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both linda.romano-at-live.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: linda.romano-at-live.com Name: Linda Romano
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, August 17, 2011 10:06 AM To: Frank Karl
Hi Linda, We can do that:
http://camcor.uoregon.edu/microanalytical/
john
At 07:00 AM 8/17/2011, microscopylistserver-noreply-at-microscopy.com wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Dear Listers I am a big fan of data, calculations or references if you can supply any. There is some lore in my facility that inverted microscopes are optically inferior to upright microscopes. I realize they have a more complicated light path. So, I'm wondering with a modern microscope how inferior, if at all, are inverted microscopes compared to upright microscopes everything else being equal (objectives, filters, camera and sample preparation). Thanks for any input Best Regards Mary
-- Mary Raven Microscopy Facility Director Neuroscience Research Institute& Molecular, Cellular& Developmental Biology Biology 2 Building, room 5173 The University of California Santa Barbara, CA 93106-5060
Mary, The customary difference is that an inverted scope is supplied with a long working distance and hence lower NA condenser. So for transmitted light modes, the NA of the inverted condenser is too small to fill the objective and hence imaging suffers. Note that this doesn't matter for fluorescence and other incident light modes, and many inverteds can be equipt with a high NA condenser and thus brought to par (of course the working distance will go down).
Hope this helps, Tobias
At 2:32 PM -0500 8/17/11, mary.raven-at-lifesci.ucsb.edu wrote: } } } Dear Listers } I am a big fan of data, calculations or references if you can supply } any. There is some lore in my facility that inverted microscopes are } optically inferior to upright microscopes. I realize they have a more } complicated light path. So, I'm wondering with a modern microscope how } inferior, if at all, are inverted microscopes compared to upright } microscopes everything else being equal (objectives, filters, camera and } sample preparation). } Thanks for any input } Best Regards } Mary } } -- } Mary Raven } Microscopy Facility Director } Neuroscience Research Institute& } Molecular, Cellular& Developmental Biology } Biology 2 Building, room 5173 } The University of California } Santa Barbara, CA 93106-5060 } } https://ucsb-microscopy.ucsd.edu/schedule/ } http://www.lifesci.ucsb.edu/mcdb/research/facilities/microscopy/microscopy.html } Phone: (805) 893 8702 } Fax: (805) 893 2005 } }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both edward.duke-at-sdsmt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: edward.duke-at-sdsmt.edu Name: Ed Duke
Organization: South Dakota School of Mines and Technology
Title-Subject: [Filtered] replacement backscattered electron detectors for FE-SEM
Message: We are planning to replace the BSD on a Zeiss Supra40 VP field emission SEM.
I am considering third-party detectors such as KE, probably a retractable 18 mm or 24 mm diode type. We need a pretty sensitive detector because of generally low probe current (~1-5 nA) and long working distance for combined BSD/EDS (15 mm).
Does anyone have any experience or suggestions with KE or other detectors, especially in FE-SEMs.
Does anyone have a plugin or macro written for ImageJ that could be used to measure the layer thickness of a multi- layered structure? I obtain images from SEM so I have grayscale images that show the layers in different shades of gray. It would be nice if the plugin could find and draw the boundary between the layers and then give me the minimum, maximum and average distance between the boundaries.
Daniel VanHart
Principal Research Support Specialist Center for Autonomous Solar Power - BI Room 2210 Binghamton University P.O. Box 6000 Binghamton, NY 13902-6000
==============================Original Headers============================== 6, 26 -- From dcvsr1-at-gmail.com Thu Aug 18 12:41:39 2011 6, 26 -- Received: from mail-yi0-f41.google.com (mail-yi0-f41.google.com [209.85.218.41]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7IHfcYQ022677 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Aug 2011 12:41:39 -0500 6, 26 -- Received: by yib2 with SMTP id 2so1807752yib.0 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Aug 2011 10:41:38 -0700 (PDT) 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=gamma; 6, 26 -- h=mime-version:from:date:message-id:subject:to:content-type 6, 26 -- :content-transfer-encoding; 6, 26 -- bh=LLxssDNSGU0I4LOnaBhD7tRbDgYYmxO2oNf83sReJp0=; 6, 26 -- b=klWRBsw5ZO+OrKd6OzBikk2O53RdPSbF+YQ0vFY0j5Y2DRO0MKNJcxL15fdq9y1c/u 6, 26 -- MRdMAnvzoqOgE+nHe4UWy09w0U3J0yRnKv8E32uV/01yLuU1JGO+C+/3v1MjGARLPb2T 6, 26 -- xFQX7SwDL3Jba4aHwpRoBr1FmmKimmmV3qCyo= 6, 26 -- Received: by 10.150.169.5 with SMTP id r5mr1004102ybe.445.1313689298108; Thu, 6, 26 -- 18 Aug 2011 10:41:38 -0700 (PDT) 6, 26 -- MIME-Version: 1.0 6, 26 -- Received: by 10.151.15.9 with HTTP; Thu, 18 Aug 2011 10:41:18 -0700 (PDT) 6, 26 -- From: Daniel VanHart {dcvsr1-at-gmail.com} 6, 26 -- Date: Thu, 18 Aug 2011 13:41:18 -0400 6, 26 -- Message-ID: {CAFMEqCeYEO_HAzLzU9C_VUXfTAhzo3p0fSeL23TB8Ybk3UAszw-at-mail.gmail.com} 6, 26 -- Subject: SEM - ImageJ Plugin or Macro for Layer Thickness 6, 26 -- To: Listserver {Microscopy-at-microscopy.com} 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7IHfcYQ022677 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both linda.romano-at-live.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: linda.romano-at-live.com Name: Linda Romano
Organization: consultant
Title-Subject: [Filtered] refurbished SEM/CL
Message: Does anyone know or have a refurbished SEM for sale? Having cathodoluminescence is a plus.
Once again I return to well of Microscopy knowledge and ask to drink not deeply, but clearly. In other words, I need help.
We have a Edwards Sputter Coater S150B. I can't get a good vacuum and I've traced it to rubber o-ring used to electrically isolated components from each other. I have a parts number, but as you know- (POOF! it's magic!) nobody has them. The part number is 11-E087-12-061. Does any one have any dimensions and hardness data on this ring so I can have a new one made? I'm reluctant to dig it out until I know I can get a new one.
Also, the high voltage cable is a cobbled together BNC connector. That needs replacement, but I would like to replace it with something a little bit more robust (we broke one of the four internal crimp connectors). Any Ideas?
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 8, 25 -- From frank_karl-at-ardl.com Fri Aug 19 09:07:42 2011 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JE7gZM007095 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 09:07:42 -0500 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 19 8, 25 -- Aug 2011 10:07:41 -0400 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 8, 25 -- Date: Fri, 19 Aug 2011 10:07:40 -0400 8, 25 -- Subject: Edwards S150B sputter coater 8, 25 -- Thread-Topic: Edwards S150B sputter coater 8, 25 -- Thread-Index: AcxeeVulo7X/4LbmRUS2ijVv+1dd3g== 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF63-at-exchange2k7.ad.ardl.com} 8, 25 -- Accept-Language: en-US 8, 25 -- Content-Language: en-US 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- acceptlanguage: en-US 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7JE7gZM007095 ==============================End of - Headers==============================
O-rings are pretty standard items. Can you get the measurements from the old O-ring? (wall thickness & either ID or OD) There should be standard "Parker" numbers of the form "2-abc". Where "a" is the wall thickness in 1/16" and "bc" describe the diameter. Note that the wall thickness for "a" numbers 0 & 1 doesn't follow the general rule.
Otherwise you can measure the groove. O-rings are generally compressed to 70% of their diameter, so the groove depth should let you calculate the wall thickness.
The Parker O-ring handbook can be found here: http://www.parker.com/literature/O-Ring%20Division%20Literature/2-xxx%20sizes.pdf
A standard industrial supply house such as Grainger should carry O-rings. I've also picked up O-rings in the plumbing section at my local home supply store.
Cheers, Henk
At 8/19/2011 10:08 AM, frank_karl-at-ardl.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Once again I return to well of Microscopy knowledge and ask to drink not deeply, but clearly. In other words, I need help. } } We have a Edwards Sputter Coater S150B. I can't get a good vacuum and I've traced it to rubber o-ring used to electrically isolated components from each other. I have a parts number, but as you know- (POOF! it's magic!) nobody has them. The part number is 11-E087-12-061. Does any one have any dimensions and hardness data on this ring so I can have a new one made? I'm reluctant to dig it out until I know I can get a new one. } } Also, the high voltage cable is a cobbled together BNC connector. That needs replacement, but I would like to replace it with something a little bit more robust (we broke one of the four internal crimp connectors). Any Ideas? } } Frank Karl } Microscopist } ARDL } 2887 Gilchrist Road } Akron, Ohio 44305 } 330-794-6600 } } } } ________________________________ } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } ==============================Original Headers============================== } 8, 25 -- From frank_karl-at-ardl.com Fri Aug 19 09:07:42 2011 } 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JE7gZM007095 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 09:07:42 -0500 } 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by } 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 19 } 8, 25 -- Aug 2011 10:07:41 -0400 } 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} } 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} } 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} } 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} } 8, 25 -- Date: Fri, 19 Aug 2011 10:07:40 -0400 } 8, 25 -- Subject: Edwards S150B sputter coater } 8, 25 -- Thread-Topic: Edwards S150B sputter coater } 8, 25 -- Thread-Index: AcxeeVulo7X/4LbmRUS2ijVv+1dd3g== } 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF63-at-exchange2k7.ad.ardl.com} } 8, 25 -- Accept-Language: en-US } 8, 25 -- Content-Language: en-US } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- acceptlanguage: en-US } 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7JE7gZM007095 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 9, 25 -- From colijn.1-at-osu.edu Fri Aug 19 10:35:27 2011 9, 25 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JFZRlL024812 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 10:35:27 -0500 9, 25 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 25 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 25 -- id {01O5174FLHLS9EE5WB-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 9, 25 -- Fri, 19 Aug 2011 11:35:26 -0400 (EDT) 9, 25 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 9, 25 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 25 -- with ESMTPA id {01O5174EW32W9F4BAT-at-ecr6.ohio-state.edu} ; Fri, 9, 25 -- 19 Aug 2011 11:35:25 -0400 (EDT) 9, 25 -- Date: Fri, 19 Aug 2011 11:35:25 -0400 9, 25 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 25 -- Subject: Re: [Microscopy] Edwards S150B sputter coater 9, 25 -- In-reply-to: {201108191408.p7JE8pKc008961-at-ns.microscopy.com} 9, 25 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 25 -- To: frank_karl-at-ardl.com, microscopy-at-microscopy.com 9, 25 -- Message-id: {4E4E82BD.9090603-at-osu.edu} 9, 25 -- MIME-version: 1.0 9, 25 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-transfer-encoding: 7bit 9, 25 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0) Gecko/20110812 Thunderbird/6.0 9, 25 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 25 -- References: {201108191408.p7JE8pKc008961-at-ns.microscopy.com} ==============================End of - Headers==============================
Karl, Edwards is saying that if you own one of their coaters, they are still providing support. I'd call them for the size (or a manual). http://www.edwardsvacuum.com/Products/List.aspx?r=125 and there is the possibility that the o-ring may be metric. I think it was Will Bigellow that put up this link https://zatkoff.com/index.asp Zatkoff seems to have an excellent selection of Parker, European metric and JIS (Japan Industrial Standard - used by JEOL) o-rings.
Ken Converse Owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: colijn.1-at-osu.edu [mailto:colijn.1-at-osu.edu] Sent: Friday, August 19, 2011 11:37 AM To: kenconverse-at-qualityimages.biz
Hi Karl,
O-rings are pretty standard items. Can you get the measurements from the old O-ring? (wall thickness & either ID or OD) There should be standard "Parker" numbers of the form "2-abc". Where "a" is the wall thickness in 1/16" and "bc" describe the diameter. Note that the wall thickness for "a" numbers 0 & 1 doesn't follow the general rule.
Otherwise you can measure the groove. O-rings are generally compressed to 70% of their diameter, so the groove depth should let you calculate the wall thickness.
The Parker O-ring handbook can be found here: http://www.parker.com/literature/O-Ring%20Division%20Literature/2-xxx%20size s.pdf
A standard industrial supply house such as Grainger should carry O-rings. I've also picked up O-rings in the plumbing section at my local home supply store.
Cheers, Henk
At 8/19/2011 10:08 AM, frank_karl-at-ardl.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Once again I return to well of Microscopy knowledge and ask to drink not deeply, but clearly. In other words, I need help. } } We have a Edwards Sputter Coater S150B. I can't get a good vacuum and I've traced it to rubber o-ring used to electrically isolated components from each other. I have a parts number, but as you know- (POOF! it's magic!) nobody has them. The part number is 11-E087-12-061. Does any one have any dimensions and hardness data on this ring so I can have a new one made? I'm reluctant to dig it out until I know I can get a new one. } } Also, the high voltage cable is a cobbled together BNC connector. That needs replacement, but I would like to replace it with something a little bit more robust (we broke one of the four internal crimp connectors). Any Ideas? } } Frank Karl } Microscopist } ARDL } 2887 Gilchrist Road } Akron, Ohio 44305 } 330-794-6600 } } } } ________________________________ } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } ==============================Original Headers============================== } 8, 25 -- From frank_karl-at-ardl.com Fri Aug 19 09:07:42 2011 } 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JE7gZM007095 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 09:07:42 -0500 } 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by } 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 19 } 8, 25 -- Aug 2011 10:07:41 -0400 } 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} } 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} } 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} } 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} } 8, 25 -- Date: Fri, 19 Aug 2011 10:07:40 -0400 } 8, 25 -- Subject: Edwards S150B sputter coater } 8, 25 -- Thread-Topic: Edwards S150B sputter coater } 8, 25 -- Thread-Index: AcxeeVulo7X/4LbmRUS2ijVv+1dd3g== } 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF63-at-exchange2k7.ad.ardl.com } } 8, 25 -- Accept-Language: en-US } 8, 25 -- Content-Language: en-US } 8, 25 -- X-MS-Has-Attach: } 8, 25 -- X-MS-TNEF-Correlator: } 8, 25 -- acceptlanguage: en-US } 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- Content-Transfer-Encoding: 8bit } 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7JE7gZM007095 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 9, 25 -- From colijn.1-at-osu.edu Fri Aug 19 10:35:27 2011 9, 25 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JFZRlL024812 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 10:35:27 -0500 9, 25 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 9, 25 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 25 -- id {01O5174FLHLS9EE5WB-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 9, 25 -- Fri, 19 Aug 2011 11:35:26 -0400 (EDT) 9, 25 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 9, 25 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 9, 25 -- with ESMTPA id {01O5174EW32W9F4BAT-at-ecr6.ohio-state.edu} ; Fri, 9, 25 -- 19 Aug 2011 11:35:25 -0400 (EDT) 9, 25 -- Date: Fri, 19 Aug 2011 11:35:25 -0400 9, 25 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 9, 25 -- Subject: Re: [Microscopy] Edwards S150B sputter coater 9, 25 -- In-reply-to: {201108191408.p7JE8pKc008961-at-ns.microscopy.com} 9, 25 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 9, 25 -- To: frank_karl-at-ardl.com, microscopy-at-microscopy.com 9, 25 -- Message-id: {4E4E82BD.9090603-at-osu.edu} 9, 25 -- MIME-version: 1.0 9, 25 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-transfer-encoding: 7bit 9, 25 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0) Gecko/20110812 Thunderbird/6.0 9, 25 -- X-Env-From: auth/colijn.1-at-osu.edu 9, 25 -- References: {201108191408.p7JE8pKc008961-at-ns.microscopy.com} ==============================End of - Headers============================== ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1392 / Virus Database: 1520/3844 - Release Date: 08/19/11
==============================Original Headers============================== 19, 28 -- From kenconverse-at-qualityimages.biz Fri Aug 19 12:11:44 2011 19, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JHBi1x010200 19, 28 -- for {microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 12:11:44 -0500 19, 28 -- X-Authority-Analysis: v=1.1 cv=aeMH4JcVOnVr0LmJAzqEvfnmJyuaZufWdlng4HTRGCk= c=1 sm=0 a=2PGrHqifizMA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=yqlxHvdyAAAA:8 a=c3R8W4AFAAAA:8 a=Zx37jsudAAAA:8 a=TI8fiyv6AAAA:8 a=YdczCmdkAAAA:8 a=x89RDgl6AAAA:8 a=oCcaPWc0AAAA:8 a=eXHZXpxNgfD3nN3x9isA:9 a=0sz16redDB-c1tuqNHkA:7 a=CjuIK1q_8ugA:10 a=c05XZbRxirYA:10 a=FyFlyU1MIKEA:10 a=5JUlSvKOtR0A:10 a=KjKrgOErgeQA:10 a=Kaox9InFUyAA:10 a=gguZerteGecA:10 a=C3J4oLNe8gwA:10 a=Bd5jmW48H-gA:10 a=RyjLG6jaqdoA:10 a=HmJ3kb8khoMA:10 a=3_2eAsHTqJoA:10 a=C6KWKcr4_fMA:10 a=G91thX30KR8A:10 a=5W7edrEKdytVqI9n:21 a=eIJfm-TSTup6fmAP:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 19, 28 -- X-Cloudmark-Score: 0 19, 28 -- X-Originating-IP: 72.224.149.57 19, 28 -- Received: from [72.224.149.57] ([72.224.149.57:1812] helo=Ken) 19, 28 -- by cdptpa-oedge02.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 19, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 19, 28 -- id 29/41-03893-F499E4E4; Fri, 19 Aug 2011 17:11:44 +0000 19, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 19, 28 -- To: "MSA Listserver" {microscopy-at-microscopy.com} 19, 28 -- Subject: RE: [Microscopy] Re: Edwards S150B sputter coater 19, 28 -- Date: Fri, 19 Aug 2011 13:11:06 -0400 19, 28 -- Message-ID: {BA570B5162314EF6A8E853C62DC41838-at-Ken} 19, 28 -- MIME-Version: 1.0 19, 28 -- Content-Type: text/plain; 19, 28 -- charset="us-ascii" 19, 28 -- X-Priority: 3 (Normal) 19, 28 -- X-MSMail-Priority: Normal 19, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 19, 28 -- Thread-Index: AcxeheYK8VqUz5nqTBm+OjUwcXNTpQADExMg 19, 28 -- In-Reply-To: {201108191537.p7JFbI8N028236-at-ns.microscopy.com} 19, 28 -- Importance: Normal 19, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 19, 28 -- Content-Transfer-Encoding: 8bit 19, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7JHBi1x010200 ==============================End of - Headers==============================
A colleague of mine is considering the purchase of an Accu-Scope 3032 inverted brightfield phase microscope from New York Microscope Co.
Personally, I have not heard anything about this brand or company and am inquiring if anyone on this list has experience with either of them. The cost ranges from $2.5 to 2.7K, which seems a bit on the low side.
Thank you and best regards from Carbondale, IL.
JB
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 18 -- From bozzola-at-siu.edu Fri Aug 19 13:16:12 2011 6, 18 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 6, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JIGBRA027607 6, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 13:16:12 -0500 6, 18 -- Received: by vwm42 with SMTP id 42so3159343vwm.0 6, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 11:16:11 -0700 (PDT) 6, 18 -- MIME-Version: 1.0 6, 18 -- Received: by 10.52.21.104 with SMTP id u8mr39253vde.409.1313777771229; Fri, 19 6, 18 -- Aug 2011 11:16:11 -0700 (PDT) 6, 18 -- Received: by 10.52.168.73 with HTTP; Fri, 19 Aug 2011 11:16:11 -0700 (PDT) 6, 18 -- Date: Fri, 19 Aug 2011 13:16:11 -0500 6, 18 -- Message-ID: {CAKeCJ9xErhmeh-Wr88rNkzmv+=rh5NAbvJ77i5mBbn9Kv=8jgg-at-mail.gmail.com} 6, 18 -- Subject: LM: inverted microscope purchase 6, 18 -- From: John Bozzola {bozzola-at-siu.edu} 6, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 6, 18 -- Content-Type: text/plain; charset=UTF-8 6, 18 -- Content-Transfer-Encoding: 8bit 6, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7JIGBRA027607 ==============================End of - Headers==============================
It looks like our HP 5000PS poster printer may be down for the count due to a hard drive failure. I am attempting to order one but the part is hard to locate.
So, in the event we have to replace it, I was wondering what 42-44 inch wide poster printer is favored by our group.
I am looking for a reliable printer with UV resistant inks.
Thank you kindly.
JB
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 7, 18 -- From bozzola-at-siu.edu Fri Aug 19 17:28:59 2011 7, 18 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7JMSxwo014485 7, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 17:28:59 -0500 7, 18 -- Received: by vwm42 with SMTP id 42so3333437vwm.0 7, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Aug 2011 15:28:59 -0700 (PDT) 7, 18 -- MIME-Version: 1.0 7, 18 -- Received: by 10.52.175.167 with SMTP id cb7mr304020vdc.74.1313792938900; Fri, 7, 18 -- 19 Aug 2011 15:28:58 -0700 (PDT) 7, 18 -- Received: by 10.52.168.73 with HTTP; Fri, 19 Aug 2011 15:28:58 -0700 (PDT) 7, 18 -- Date: Fri, 19 Aug 2011 17:28:58 -0500 7, 18 -- Message-ID: {CAKeCJ9yyw2H=m=E38z-hMgyOPkN1AqKD5NvZPUu-b2yx-1qJmg-at-mail.gmail.com} 7, 18 -- Subject: poster printer recommendations 7, 18 -- From: John Bozzola {bozzola-at-siu.edu} 7, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 7, 18 -- Content-Type: text/plain; charset=UTF-8 7, 18 -- Content-Transfer-Encoding: 8bit 7, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7JMSxwo014485 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both exploratorium-at-tiscali.it as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: exploratorium-at-tiscali.it Name: Giovanni De Caro
Organization: Exploratorium Casalciprano - Campobasso Title-Subject: [Filtered] PHILIPS 505 SEM
Message: Hi all. I have been offered at a low price a dismissed Philips 505 sem which I would like to use for educational purposes (science museum for youngsters). The SEM has an EDAX and the seller says it was working when shut off a couple of years ago. I would like to know from some microscopist who has used or currently uses this instrument which are the main components of this SEM which are more prone to go out of service and if this is the kind of instrument which rarely needs technical assistance (a "workhorse") or, on the other hand, if it is a delicate SEM which with years tends to bee too expensive to repair and maintain . To end , I need to know which are the things to inspect first in order to understand if the instrument has undergone fatal breakdowns which renders the SEM non reparable if not at a very high cost. Thank you for your kind help. Giovanni, Italia
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scopes.master-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scopes.master-at-gmail.com Name: Hamid R. Shakeri
Organization: Aviceena Metallurgical Research Services
Title-Subject: [Filtered] Veteran Expert on Cambridge Stereoscan 180
Message: Are there any Veterans of Electron Microscopy with extensive experience with Cambridge Stereoscan 180 around here? I am trying to make one of these works again and need help in this regard. Thank you!
I need advise on a new TEM sample. This is nano aluminum particles coated (or so we hope) with nitrocellulose. It is easy to suspend the nanoAl in ETOH or MeOH using sonicating and then depositing it on a coated grid for imaging.
Problem comes in visualizing the nitrocellulose coating. The nanoAl cannot be diluted in water or it will react releasing hydrogen. What I need is the equivalent of a non-aqueous negative stain to see the coating. I have tried aqueous UA and also UA in 70% MeOH without getting the desired negative staining.
Is it possible to get negative staining with non-aqueous systems? Any suggestions as to how to do it if it is possible would be appreciated as would other suggestions on imaging these particles.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 10, 29 -- From dsherman-at-purdue.edu Mon Aug 22 21:34:37 2011 10, 29 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7N2YZJG017889 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 22 Aug 2011 21:34:37 -0500 10, 29 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 10, 29 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p7N2YTBd005277 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 22 Aug 2011 22:34:29 -0400 10, 29 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.54]) by WPPEXHUB04H.purdue.lcl 10, 29 -- ([::1]) with mapi; Mon, 22 Aug 2011 22:34:29 -0400 10, 29 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 29 -- CC: David Reese {reesed-at-purdue.edu} 10, 29 -- Date: Mon, 22 Aug 2011 22:34:28 -0400 10, 29 -- Subject: NanoAl-nitrocellulose staining 10, 29 -- Thread-Topic: NanoAl-nitrocellulose staining 10, 29 -- Thread-Index: AcxhPS4GW3qfIk2cAUivzQzWH7uskA== 10, 29 -- Message-ID: {CA7889F4.11319%dsherman-at-purdue.edu} 10, 29 -- Accept-Language: en-US 10, 29 -- Content-Language: en-US 10, 29 -- X-MS-Has-Attach: 10, 29 -- X-MS-TNEF-Correlator: 10, 29 -- user-agent: Microsoft-Entourage/13.10.0.110428 10, 29 -- acceptlanguage: en-US 10, 29 -- Content-Type: text/plain; charset="us-ascii" 10, 29 -- MIME-Version: 1.0 10, 29 -- X-PMX-Version: 5.5.9.388399 10, 29 -- X-PerlMx-Virus-Scanned: Yes 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7N2YZJG017889 ==============================End of - Headers==============================
Well, this isn't negative staining, but perhaps you could try a high-angle (75-80 deg) Pt-C shadow. I think a rotary shadow would give you the best results (although a unidirectional shadow might be enough to demonstrate the presence of the nitrocellulose layer). I'm thinking just a light coating, so you can see a clear zone of nitrocellulose centered around each black aluminum particle, that would be absent on similar, non-coated particles...
Andy Bowling
_____________________ Andrew J Bowling, PhD Discovery R&D Dow AgroSciences 9330 Zionsville Rd Indianapolis, IN 46268 317-337-3878
-----Original Message----- X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu] Sent: Monday, August 22, 2011 10:46 PM To: Bowling, Andrew (AJ)
Hi all,
I need advise on a new TEM sample. This is nano aluminum particles coated (or so we hope) with nitrocellulose. It is easy to suspend the nanoAl in ETOH or MeOH using sonicating and then depositing it on a coated grid for imaging.
Problem comes in visualizing the nitrocellulose coating. The nanoAl cannot be diluted in water or it will react releasing hydrogen. What I need is the equivalent of a non-aqueous negative stain to see the coating. I have tried aqueous UA and also UA in 70% MeOH without getting the desired negative staining.
Is it possible to get negative staining with non-aqueous systems? Any suggestions as to how to do it if it is possible would be appreciated as would other suggestions on imaging these particles.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 10, 29 -- From dsherman-at-purdue.edu Mon Aug 22 21:34:37 2011 10, 29 -- Received: from mailhub128.itcs.purdue.edu (mailhub128.itcs.purdue.edu [128.210.5.128]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7N2YZJG017889 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 22 Aug 2011 21:34:37 -0500 10, 29 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 10, 29 -- by mailhub128.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p7N2YTBd005277 10, 29 -- for {microscopy-at-microscopy.com} ; Mon, 22 Aug 2011 22:34:29 -0400 10, 29 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.54]) by WPPEXHUB04H.purdue.lcl 10, 29 -- ([::1]) with mapi; Mon, 22 Aug 2011 22:34:29 -0400 10, 29 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 29 -- CC: David Reese {reesed-at-purdue.edu} 10, 29 -- Date: Mon, 22 Aug 2011 22:34:28 -0400 10, 29 -- Subject: NanoAl-nitrocellulose staining 10, 29 -- Thread-Topic: NanoAl-nitrocellulose staining 10, 29 -- Thread-Index: AcxhPS4GW3qfIk2cAUivzQzWH7uskA== 10, 29 -- Message-ID: {CA7889F4.11319%dsherman-at-purdue.edu} 10, 29 -- Accept-Language: en-US 10, 29 -- Content-Language: en-US 10, 29 -- X-MS-Has-Attach: 10, 29 -- X-MS-TNEF-Correlator: 10, 29 -- user-agent: Microsoft-Entourage/13.10.0.110428 10, 29 -- acceptlanguage: en-US 10, 29 -- Content-Type: text/plain; charset="us-ascii" 10, 29 -- MIME-Version: 1.0 10, 29 -- X-PMX-Version: 5.5.9.388399 10, 29 -- X-PerlMx-Virus-Scanned: Yes 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7N2YZJG017889 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 33 -- From AJBowling-at-dow.com Tue Aug 23 08:27:43 2011 26, 33 -- Received: from mail171.messagelabs.com (mail171.messagelabs.com [216.82.253.243]) 26, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7NDRgx6011124 26, 33 -- for {microscopy-at-microscopy.com} ; Tue, 23 Aug 2011 08:27:43 -0500 26, 33 -- X-VirusChecked: Checked 26, 33 -- X-Env-Sender: AJBowling-at-dow.com 26, 33 -- X-Msg-Ref: server-15.tower-171.messagelabs.com!1314106045!22670435!9 26, 33 -- X-StarScan-Version: 6.2.17; banners=-,-,- 26, 33 -- X-Originating-IP: [204.136.184.20] 26, 33 -- Received: (qmail 27985 invoked from network); 23 Aug 2011 13:27:37 -0000 26, 33 -- Received: from maila.dow.com (HELO USFRPMDOWS002.dow.com) (204.136.184.20) 26, 33 -- by server-15.tower-171.messagelabs.com with RC4-SHA encrypted SMTP; 23 Aug 2011 13:27:37 -0000 26, 33 -- Received: from USMDLMDOWX032.dow.com ([163.198.215.63]) by USFRPMDOWS002.dow.com with Microsoft SMTPSVC(6.0.3790.4675); 26, 33 -- Tue, 23 Aug 2011 08:27:06 -0500 26, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 26, 33 -- Content-class: urn:content-classes:message 26, 33 -- MIME-Version: 1.0 26, 33 -- Content-Type: text/plain; 26, 33 -- charset="us-ascii" 26, 33 -- Subject: RE: [Microscopy] NanoAl-nitrocellulose staining 26, 33 -- Date: Tue, 23 Aug 2011 09:27:04 -0400 26, 33 -- Message-ID: {B72477374D7A74408DAC63801A0FDEA1029F57C1-at-USMDLMDOWX032.dow.com} 26, 33 -- In-Reply-To: {201108230246.p7N2k81e031087-at-ns.microscopy.com} 26, 33 -- X-MS-Has-Attach: 26, 33 -- X-MS-TNEF-Correlator: 26, 33 -- Thread-Topic: [Microscopy] NanoAl-nitrocellulose staining 26, 33 -- Thread-Index: AcxhPtFYjD8OIEt5SyyjRmruycJLQAAV4a5A 26, 33 -- References: {201108230246.p7N2k81e031087-at-ns.microscopy.com} 26, 33 -- From: "Bowling, Andrew (AJ)" {AJBowling-at-dow.com} 26, 33 -- To: {microscopy-at-microscopy.com} 26, 33 -- X-OriginalArrivalTime: 23 Aug 2011 13:27:06.0450 (UTC) FILETIME=[5A481B20:01CC6198] 26, 33 -- Content-Transfer-Encoding: 8bit 26, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7NDRgx6011124 ==============================End of - Headers==============================
This is the last week for the registration for the workshop at the Center for Nanophase Materials Sciences: Advanced Scanning Probe Microscopies at the CNMS: Materials Structure and Function from Atomic to Micron Scales (September 21-22, 2011).
The registration information is available at: https://www.ornl.gov/cnms/2011/index.cfm A number of travel fellowships will be available for the graduate students on the first come-first served basis (contact Art Baddorf, baddorfap-at-ornl.gov, for details). Several of the companies will run the demos of their latest SPM instruments. The tours of ORNL SPM machines will be available.
The workshop features presentations by CNMS staff members and SPM industry focused on technical capabilities developed and/or available at CNMS. These include:
• Atomic and vibrational imaging by low-temperature high magnetic field STM • Transport characterization by 4-probe STM/SEM • Scanning Electron Microscopy with Polarization Analysis • Piezoresponse Force Microscopy and Spectroscopy • Band Excitation SPMs for thermal, magnetic, and mechanical property mapping • Electrochemical Strain Microscopy of Li-ion and oxygen conductors • Microwave imaging and spectroscopy
This workshop will also feature presentation by leading SPM vendors devoted to the latest advances in SPM modes. Ultimately, we aim to build a network of advanced SPM practitioners to promote rapid dissemination of theoretical knowledge, experimental protocols, and novel technique development in these rapidly growing areas.
For more information and registration, please check the CNMS web site at www.cnms.ornl.gov or contact the organizers at sergei2-at-ornl.gov or baddorfap-at-ornl.gov.
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 11, 26 -- From sergei2-at-ornl.gov Tue Aug 23 15:24:13 2011 11, 26 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7NKOC7c006346 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 23 Aug 2011 15:24:12 -0500 11, 26 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 11, 26 -- by emroute3.ornl.gov (PMDF V6.5-x6 #31890) 11, 26 -- with ESMTP id {0LQE00662E0CV8-at-emroute3.ornl.gov} for 11, 26 -- microscopy-at-microscopy.com; Tue, 23 Aug 2011 16:24:12 -0400 (EDT) 11, 26 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 11, 26 -- (PMDF V6.5-x6 #31890) id {0LQE00701E0C6L-at-emroute3.ornl.gov} for 11, 26 -- microscopy-at-microscopy.com; Tue, 23 Aug 2011 16:24:12 -0400 (EDT) 11, 26 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 11, 26 -- by emroute3.ornl.gov (PMDF V6.5-x6 #31890) 11, 26 -- with ESMTPS id {0LQE006BKE0CBW-at-emroute3.ornl.gov} for 11, 26 -- microscopy-at-microscopy.com; Tue, 23 Aug 2011 16:24:12 -0400 (EDT) 11, 26 -- Date: Tue, 23 Aug 2011 16:24:12 -0400 11, 26 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 11, 26 -- Subject: CNMS SPM workshop - updates 11, 26 -- To: microscopy-at-microscopy.com 11, 26 -- Cc: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 11, 26 -- Message-id: {4E540C6C.5020403-at-ornl.gov} 11, 26 -- MIME-version: 1.0 11, 26 -- Content-type: text/plain; charset=windows-1252; format=flowed 11, 26 -- Content-transfer-encoding: 8BIT 11, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.20) 11, 26 -- Gecko/20110804 Lightning/1.0b2 Thunderbird/3.1.12 ==============================End of - Headers==============================
I've just unearthed an ancient Sorvall/Porta-Blum MT-2 and much newer LKB 7801A knifemaker. I used both of these some 15 years ago and they were operational, but as I have no glass strips I'm unable to test either one now. Both appear to have all their parts (The MT-2 is missing the "Reset" button, but the lever that the button actually moves is accessible). The knifemaker is fairly pristine (I see one trim screw missing on the back). In the past the microtome was marginally capable of cutting ultrathin sections, but as it appears to have been "rode hard and put away wet" so to speak in its life in other labs, I'm not sure about it now.
Any takers? I can give them away if someone pays shipping, but will also consider the highest cash(!) or trade offer. We're primarily a JEOL SEM lab, so I'd be interested in things like unused "K"-type filaments, 10x10 mm stubs, etc. that you may have hanging around your neck and want to get rid of. Be aware that the microtome is boat-anchor heavy, so I would imagine shipping costs would be significant.
If I don't hear from anybody within a week, at least the microtome is headed for a better place (i.e., the dumpster).
Thanks,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
The Hooke College of Applied Sciences, located in Westmont, IL, is offering a Transmission Electron Microscopy short course October 18-20, 2011. In addition to lectures, this course emphasizes hands-on training using state of the art equipment. For further details and registration information, please visit our website:
http://www.hookecollege.com/
You can also contact me directly if you have questions about the course.
Regards,
Elaine
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 11, 26 -- From eschumacher-at-mccrone.com Wed Aug 24 08:39:22 2011 11, 26 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ODdMne010714 11, 26 -- for {microscopy-at-microscopy.com} ; Wed, 24 Aug 2011 08:39:22 -0500 11, 26 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 11, 26 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Wed, 24 Aug 2011 08:39:20 11, 26 -- -0500 11, 26 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 26 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 26 -- Date: Wed, 24 Aug 2011 08:39:20 -0500 11, 26 -- Subject: Short Course Announcement - Transmission Electron Microscopy 11, 26 -- Thread-Topic: Short Course Announcement - Transmission Electron Microscopy 11, 26 -- Thread-Index: AcxiYzoes/bl0f8+Su+jtAgvE4xpOQ== 11, 26 -- Message-ID: {874B1DB532886444A60A015EE363493F4854F45EBE-at-mccronemsg07.tmg.mccrone.com} 11, 26 -- Accept-Language: en-US 11, 26 -- Content-Language: en-US 11, 26 -- X-MS-Has-Attach: 11, 26 -- X-MS-TNEF-Correlator: 11, 26 -- x-vipre-scanned: 0A96462300280C0A964770 11, 26 -- x-ninja-pim: Scanned by Ninja 11, 26 -- x-ninja-attachmentfiltering: (no action) 11, 26 -- acceptlanguage: en-US 11, 26 -- Content-Type: text/plain; charset="us-ascii" 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7ODdMne010714 ==============================End of - Headers==============================
I figured the knifemaker would be popular. I've already promised it to someone on a "free" basis but with our budget constraints have reserved the right to give it to someone else who can trade for something we need. So dig through your closets - new JEOL "K" filaments don't grow on trees, but they do tend to lurk in forgotten dark corners.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries.
The Opportunity:
The company currently has an opening for a Head of Business Segment Clinical Imaging. This position can be based in Wetzlar, Germany or Newcastle, UK. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary.
Base salary is commensurate with experience plus bonus
Primary Responsibilities:
The Head of Business Segment Clinical Imaging will maintain ownership of business in clinical customer segment, creating transparency in top line, and maximizing business in that market. This position will manage all Product Marketing and R&D functions related to business segment. The Head of Business Segment will also maintain ownership of defined product range over complete product life cycle, product and roadmap definition. This role will plan and execute expansion into new application areas, ownership of market and competitive intelligence.
Additional Responsibilities: - Setting up the strategic plan and budget for Clinical Imaging by leveraging microscopy technology into the niche application space - Define product developments and product improvements to be made, drive the product roadmap in the segment in order to guarantee maximum business volume and margin, as well as customer satisfaction with the company's product - Evaluation and selection of innovative ideas, in order to build up a technical concept for the product, teamwork with R&D in order to realize innovative projects and bring them to the market - Preparation and planning of market launches and presentations together with the Selling Unit, in order to successfully launch new products and sell them to the customers - Manage the R&D Manager and align R&D strategy and execution with business plan and budget; Ensure deadlines for R&D roadmaps are met - Responsible for global pricing strategy to ensure market competitive prices - Management and development of associates in two sites (UK and Germany) in order to ensure high motivation and high performance within the team by using provided HR tools and processes
Education and Experience Required:
- Studies in Natural Sciences - Knowledge in life science, medical devices and economics - Experience in International Marketing - Experience in Microscopy is highly desired - Track record in successful product definition and market launches - Leadership skills to lead and strengthen the product management and application terms - Excellent English skills; Fluency in German is a plus but not required
If you meet and/or exceed the experience criteria, please submit your resume for review by clicking on the link below (you may have to copy and paste it into your browser) or by emailing your info to Christy Edwards at ce-at-personifysearch.com. We wish everyone the best of luck. Unfortunately only qualified candidates will be considered.
http://tinyurl.com/LEU-HeadofBusSeg-GE-UK
Christy Edwards Sr. e-Recruitment Consultant Personify 5020 Weston Parkway Suite 315 Cary, North Carolina 27513 (Tel) 800.875.6188 direct ext 109 (Fax) 919.460.8726 www.personifysearch.com
==============================Original Headers============================== 19, 21 -- From ce-at-personifysearch.com Fri Aug 26 10:44:48 2011 19, 21 -- Received: from na3sys010aog110.obsmtp.com (na3sys010aog110.obsmtp.com [74.125.245.88]) 19, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p7QFilof012734 19, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Aug 2011 10:44:48 -0500 19, 21 -- Received: from mail-qw0-f53.google.com ([209.85.216.53]) (using TLSv1) by na3sys010aob110.postini.com ([74.125.244.12]) with SMTP 19, 21 -- ID DSNKTle/b9ZZjKq7ARcgbMcekJTug2a3rRGC-at-postini.com; Fri, 26 Aug 2011 08:44:48 PDT 19, 21 -- Received: by mail-qw0-f53.google.com with SMTP id 7so2513132qwb.12 19, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Aug 2011 08:44:47 -0700 (PDT) 19, 21 -- Received: by 10.42.156.5 with SMTP id x5mr1297566icw.147.1314373486742; Fri, 19, 21 -- 26 Aug 2011 08:44:46 -0700 (PDT) 19, 21 -- From: Christy Edwards {ce-at-personifysearch.com} 19, 21 -- MIME-Version: 1.0 19, 21 -- X-Mailer: Microsoft Office Outlook 12.0 19, 21 -- Thread-Index: AcxkBxdPXDU5T2d8SLKz+pefF+WICg== 19, 21 -- Date: Fri, 26 Aug 2011 11:44:50 -0400 19, 21 -- Message-ID: {ac119c78f1e87864f3848d68de301d80-at-mail.gmail.com} 19, 21 -- Subject: Job Opportunity - Head of Business Segment Clinical Imaging 19, 21 -- To: Microscopy-at-microscopy.com 19, 21 -- Content-Type: text/plain; charset=ISO-8859-1 19, 21 -- Content-Transfer-Encoding: 8bit 19, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7QFilof012734 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both araz79kh as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: araz79kh Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] set up and analyzing problem
Message: Hi All
I worked with SX100 but it makes more trouble for us and unfortunately it isn't user-friendly. now I have problem in set up the system. when I calibrated the system and save it after load the considering setup it is failed and never calibrated especially when choosing the point for analyzing and acquire it. and the error comes: error from setup: anomaly of high voltage or anomaly of gun current. I changed the filament many times but nothing was happened and previous error is repeated again. why is it occur? what can I do to fix this problem? what is the good shape for beam when we write gun scan high and low scan high? please help me in this cases.
Debby --- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
==============================Original Headers============================== 10, 28 -- From dsherman-at-purdue.edu Sat Aug 27 10:49:14 2011 10, 28 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7RFnEZR029684 10, 28 -- for {microscopy-at-microscopy.com} ; Sat, 27 Aug 2011 10:49:14 -0500 10, 28 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) 10, 28 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p7RFnEGr019615 10, 28 -- for {microscopy-at-microscopy.com} ; Sat, 27 Aug 2011 11:49:14 -0400 10, 28 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.54]) by WPPEXHUB02F.purdue.lcl 10, 28 -- ([::1]) with mapi; Sat, 27 Aug 2011 11:49:13 -0400 10, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 28 -- Date: Sat, 27 Aug 2011 11:49:12 -0400 10, 28 -- Subject: Lab director-Job posting 10, 28 -- Thread-Topic: Lab director-Job posting 10, 28 -- Thread-Index: Acxk0N2OFWnaXZraNEyO6ujCqeASRA== 10, 28 -- Message-ID: {CA7E8A38.11635%dsherman-at-purdue.edu} 10, 28 -- Accept-Language: en-US 10, 28 -- Content-Language: en-US 10, 28 -- X-MS-Has-Attach: 10, 28 -- X-MS-TNEF-Correlator: 10, 28 -- user-agent: Microsoft-Entourage/13.10.0.110428 10, 28 -- acceptlanguage: en-US 10, 28 -- Content-Type: text/plain; charset="Windows-1252" 10, 28 -- MIME-Version: 1.0 10, 28 -- X-PMX-Version: 5.5.9.388399 10, 28 -- X-PerlMx-Virus-Scanned: Yes 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7RFnEZR029684 ==============================End of - Headers==============================
I want to thank everyone with their suggestions about how to get our sputter up and running. What did we find?
Well, the vacuum fixed itself. Go figure. Must have been a particle of crud on a gasket, we thought they looked clean, but who knows. As far as the gold coating, it was a bad spot in the high voltage co-axial cable creating a short. We lengthen it. I'd still line to find the original electrical cap connection, but it is working like a champ.
Thanks again for the ideas and information.
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 8, 25 -- From frank_karl-at-ardl.com Mon Aug 29 09:10:08 2011 8, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7TEA753026310 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 29 Aug 2011 09:10:07 -0500 8, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 8, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Mon, 29 8, 25 -- Aug 2011 10:10:06 -0400 8, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 8, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 8, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 8, 25 -- Date: Mon, 29 Aug 2011 10:10:06 -0400 8, 25 -- Subject: Thanks! 8, 25 -- Thread-Topic: Thanks! 8, 25 -- Thread-Index: AcxmVVpSDpLG7pH5Tt6FgTcejo5kvw== 8, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF68-at-exchange2k7.ad.ardl.com} 8, 25 -- Accept-Language: en-US 8, 25 -- Content-Language: en-US 8, 25 -- X-MS-Has-Attach: 8, 25 -- X-MS-TNEF-Correlator: 8, 25 -- acceptlanguage: en-US 8, 25 -- Content-Type: text/plain; charset="iso-8859-1" 8, 25 -- MIME-Version: 1.0 8, 25 -- Content-Transfer-Encoding: 8bit 8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7TEA753026310 ==============================End of - Headers==============================
We have in our laboratory one old GATAN MSC 1kx1k CCD camera + Oxford Instruments Isis X-ray EDS electronics (without the detector), both of ~1998, to donate. They were both used on a TEM JEOL 2010. They were in good state and functioning when they were replaced some years ago. They are at the Paul Scherrer Institute in Switzerland. We donate them; shipping and handling to be paid.
Please contact me if you are interested.
Best wishes,
Minikus Eugenia
CRPP-EPFL/Fusion Technology
5232 Villigen PSI / Switzerland
Tel +41 56 310 47 07
Fax +41 56 310 45 29
==============================Original Headers============================== 12, 30 -- From eugenia.minikus-at-psi.ch Tue Aug 30 07:28:42 2011 12, 30 -- Received: from esa01.psi.ch (esa01.psi.ch [192.33.126.171]) 12, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7UCSfEI027947 12, 30 -- for {Microscopy-at-microscopy.com} ; Tue, 30 Aug 2011 07:28:42 -0500 12, 30 -- X-IronPort-Anti-Spam-Filtered: true 12, 30 -- X-IronPort-Anti-Spam-Result: AqAGALbWXE6Bgb82/2dsb2JhbABDmHaPH3eBQQEBAQMwWQIBKgYMDAYBVgEBBBvAcYVtYASHN5EEi3A 12, 30 -- X-IronPort-AV: E=Sophos;i="4.68,302,1312149600"; 12, 30 -- d="scan'208";a="46850242" 12, 30 -- Received: from mailsend02.psi.ch ([129.129.191.54]) 12, 30 -- by esa01-i.psi.ch with ESMTP; 30 Aug 2011 14:28:41 +0200 12, 30 -- Received: from MAILBOX0B.psi.ch ([129.129.190.194]) by MAILSEND02.psi.ch with Microsoft SMTPSVC(6.0.3790.4675); 12, 30 -- Tue, 30 Aug 2011 14:28:40 +0200 12, 30 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 12, 30 -- Content-class: urn:content-classes:message 12, 30 -- MIME-Version: 1.0 12, 30 -- Content-Type: text/plain; 12, 30 -- charset="iso-8859-1" 12, 30 -- Subject: GATAN MSC 1k CCD camera + Oxford Instr Isis X-ray EDS electronic ~1998 to give 12, 30 -- Date: Tue, 30 Aug 2011 14:27:33 +0200 12, 30 -- Message-ID: {0C9CAFE8C4E5CA49884E636AE1D7CD6903F34B76-at-MAILBOX0B.psi.ch} 12, 30 -- X-MS-Has-Attach: 12, 30 -- X-MS-TNEF-Correlator: 12, 30 -- Thread-Topic: GATAN MSC 1k CCD camera + Oxford Instr Isis X-ray EDS electronic ~1998 to give 12, 30 -- Thread-Index: AcxnEDGnIC7eEzRqQli2cC3DsrC/4g== 12, 30 -- References: {4E5B8F23.4030905-at-psi.ch} 12, 30 -- From: "Minikus Eugenia" {eugenia.minikus-at-psi.ch} 12, 30 -- To: {Microscopy-at-microscopy.com} 12, 30 -- X-OriginalArrivalTime: 30 Aug 2011 12:28:41.0179 (UTC) FILETIME=[59DE72B0:01CC6710] 12, 30 -- Content-Transfer-Encoding: 8bit 12, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7UCSfEI027947 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both martin.saunders-at-uwa.edu.au as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: martin.saunders-at-uwa.edu.au Name: Martin Saunders
Organization: The University of Western Australia
Title-Subject: [Filtered] Job opening: EM academic (Assistant Prof/Associate Prof)
Message: The Centre for Microscopy, Characterisaton and Analysis (CMCA) at The University of Western Australia is seeking suitable applicants for a tenurable academic position (Assistant Prof/Associate Prof) to join the CentreÂ’s electron microscopy group, providing support to researchers broadly across the physical sciences/materials science and engineering disciplines (potentially in both SEM and TEM). A key role will be to provide renewed academic leadership in the field of scanning electron microscopy as a consequence of impending retirement. The CMCA is a major central university facility that supports the research-intensive role of the university, forming a nexus for collaborative and interdisciplinary research linked to applications of our extensive microscopy and microanalysis capabilities, which include SEM, TEM, EPMA, ion probes, optical, confocal, flow cytometry, cell sorting, XRD, NMR and mass spec.
The successful candidate will have a background in the application of electron microscopy in the physical sciences. They will be expected to lead SEM-related activity in the Centre, including the provision of teaching/training, and driving future plans for SEM capabilities at the university. They will be encouraged to develop their own research programmes while also establishing collaborative and interdisciplinary research activities with local researchers.
More information on this position can be found at https://www.his.admin.uwa.edu.au/jobvacs/external/academic/doc/doc2123955.RTF
Queries can be directed to our Centre Director (Prof David Sampson, david.sampson-at-uwa.edu.au) or myself (martin.saunders-at-uwa.edu.au).
Regards,
Martin.
*****************************************
Professor Martin Saunders, Deputy Director, Centre for Microscopy, Characterisation and Analysis, M010, The University of Western Australia, Crawley, Western Australia 6009, Australia.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpflugheber-at-stlawu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I'm beginning preparations for a new class, and I would like to preview some histology textbooks. The class will be for undergraduates, probably at the 200 level, and will be a combination of histology/methods and microscopy. Does anyone have any favorites that they would recommend I preview? Thanks very much.
One text we have used for years is: Histology: A text and Atlas - Johannes A.G. Rhodin - Oxf Uni Press published 1974 - I'm not sure if it is still in print but used to be our "bible". Very good for routine TEM mammalian histology as a histology atlas.
There appears to be a web version of some of the pictures in an on-line text http://projects.galter.northwestern.edu/rhodin/
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: 30 August 2011 22:52 To: Malcolm Haswell
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpflugheber-at-stlawu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I'm beginning preparations for a new class, and I would like to preview some histology textbooks. The class will be for undergraduates, probably at the 200 level, and will be a combination of histology/methods and microscopy. Does anyone have any favorites that they would recommend I preview? Thanks very much.
My lab works with pharmaceutical powders and we almost always perform some SEM on them. While the powders are technically stuck to the posts with adhesive I assume some loose powder is sucked up through the vacuum system while sputter coating. It appears to me that the sputter coater has no filtration system of any kind and could technically vent any powders back into the lab through the exhaust. It's an older Hummer 6.2 system (no digital display so I assume it's at least 10 years old), the exhaust port we want to cover is 6inchesx6inches.
I'd like to put a HEPA filter on the exhaust port but I contacted Anatech and they didn't know a supplier for a housing like this. Most HEPA filter housings I'm finding online are way too large for the exhaust port, has anyone does this? Did you make your own filter housing or is there a supplier for this kind of thing?
Thanks,
Annie
Disclaimer - August 31, 2011 LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling Bend Research Inc (541) 382-4100.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:25:56 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLPuKx022026 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id A25B9404A8A 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id TEfrgTipHaWS for {microscopy-at-microscopy.com} ; 12, 25 -- Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) 12, 25 -- by znl.com (Postfix) with ESMTPA id B2D76404A7F 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:55 -0500 (CDT) 12, 25 -- Message-ID: {4E5EA6E1.8020005-at-microscopy.com} 12, 25 -- Date: Wed, 31 Aug 2011 23:25:53 +0200 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 -- Subject: [Filtered] HEPA filter system for sputter coater 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both prlax-at-mindspring.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: prlax-at-mindspring.com Name: David OHara
Organization: Parallax Research, Inc.
Title-Subject: [Filtered] WDS capabilities
Message: First, I want to thank Dr Peter Ingram for reminding me of this listServer. Next, my primary interest is in developing new WDS type instrumentation so I like to see questions concerning WDS and things it can do and potential applications. For example, there was a thread a couple of months ago concerning measuring Na and Zn and someone asked if WDS could resolve this overlap. The answer is "Yes" if the right diffractor is used (TAP will work but the multilayer diffractors will still give an overlap) Not being an EM trained person, the needs for WDS are often not obvious to me so this forum will be especially valueable. So I have a few questions for EM users: 1: Is anybody interested in measuring Li in their SEM? 2: I was told by somebody that the Li line at 52 eV was due to a "forbidden" transition so we would never see it....correct? 3: Why do I never hear about WDS on TEMs?
Somewhere inside of the sputter coater is a roughing pump with the exhaust port that is significantly smaller then 6"x6" - in fact it may be small enough to connect a PVC hose of a suitable diameter. If you have difficulty locating pump exhaust port then try to speak with someone who services it. First page of Google search had link to Ladd Research in VT - these guys are still selling Hummer 6.2 coaters and I know them as very responsive and helpful. No any connections here - just a satisfied customer.
The ideal approach would be to connect a (clear PVC?) hose to the exhaust port of the rouging pump in your coater and route it into the safety exhaust of your lab. If you do not have safety exhaust in the lab, then get a couple of filter housings for the water supply lines, of the kind sold for whole-house water filtration (Home Depot or online), connect them in series and fit first one with 1um filter cartridge and second with activated charcoal filter cartridge. These water filters have very low flow resistance, so pumping efficiency of your coater will not be affected. After 1um followed by charcoal your air exhaust should be much cleaner then it is now...
If you do not want to mess with plumbing and insist on covering 6"x6" vent port of the sputter coater enclosure, then it is very easy to make HEPA filters of custom size.
Simply buy larger HEPA filter of your liking, cut it to size with scissors, glue solid edge around its perimeter (I like to use waterproofing Al tape from 3M - its glue remains tacky for years) and seal border to the filter with silicone caulk to prevent air from filtering around. My laminar flow hood is using non-standard filters, so I am going through filter-trimming exercise every 6 months to one year, depending on usage. After some practice it takes about 15 minutes of work to get perfect filter of a custom size. Stick your DIY filter to the coater with solid perimeter of double-sided tape and secure with Al tape for safety. If you are careful then it may even look pretty...
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 8/31/2011 5:26 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi everyone, } } } My lab works with pharmaceutical powders and we almost always perform } some SEM on them. While the powders are technically stuck to the posts } with adhesive I assume some loose powder is sucked up through the vacuum } system while sputter coating. It appears to me that the sputter coater } has no filtration system of any kind and could technically vent any } powders back into the lab through the exhaust. It's an older Hummer 6.2 } system (no digital display so I assume it's at least 10 years old), the } exhaust port we want to cover is 6inchesx6inches. } } } I'd like to put a HEPA filter on the exhaust port but I contacted } Anatech and they didn't know a supplier for a housing like this. Most } HEPA filter housings I'm finding online are way too large for the } exhaust port, has anyone does this? Did you make your own filter housing } or is there a supplier for this kind of thing? } } } Thanks, } } Annie } } } } Disclaimer - August 31, 2011 LEGAL NOTICE: This message (and/or any } attachments accompanying it) is confidential and proprietary. It is } intended for the addressee(s) only. Access to this e-mail by anyone } else is unauthorized. If you are not an addressee any disclosure, } copying, or distribution of the contents of this e-mail (and any } attachments) or any action taken (or not taken) in reliance upon this } message is unauthorized and may be unlawful. If you are not an } addressee, please contact the sender immediately by calling Bend } Research Inc (541) 382-4100. } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:25:56 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLPuKx022026 } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id A25B9404A8A } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id TEfrgTipHaWS for {microscopy-at-microscopy.com} ; } 12, 25 -- Wed, 31 Aug 2011 16:25:56 -0500 (CDT) } 12, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id B2D76404A7F } 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:55 -0500 (CDT) } 12, 25 -- Message-ID: {4E5EA6E1.8020005-at-microscopy.com} } 12, 25 -- Date: Wed, 31 Aug 2011 23:25:53 +0200 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: [Filtered] HEPA filter system for sputter coater } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 38 -- From vray-at-partbeamsystech.com Wed Aug 31 17:06:58 2011 8, 38 -- Received: from nm15.access.bullet.mail.mud.yahoo.com (nm15.access.bullet.mail.mud.yahoo.com [66.94.237.216]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p7VM6w6X027256 8, 38 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 17:06:58 -0500 8, 38 -- Received: from [66.94.237.192] by nm15.access.bullet.mail.mud.yahoo.com with NNFMP; 31 Aug 2011 22:06:57 -0000 8, 38 -- Received: from [98.139.221.63] by tm3.access.bullet.mail.mud.yahoo.com with NNFMP; 31 Aug 2011 22:06:57 -0000 8, 38 -- Received: from [127.0.0.1] by smtp104.biz.mail.bf1.yahoo.com with NNFMP; 31 Aug 2011 22:06:56 -0000 8, 38 -- X-Yahoo-Newman-Id: 992615.39121.bm-at-smtp104.biz.mail.bf1.yahoo.com 8, 38 -- X-Yahoo-Newman-Property: ymail-3 8, 38 -- X-YMail-OSG: Rn7EDO8VM1lMwz47dFh4gv1vUloxEimhmTmPc0dQ.wGUXNQ 8, 38 -- 56yGJsfFwJQCwhr1iP9DAOxqnZTn83NK029wIzXJs6bgLCSXi74IfPNi_psi 8, 38 -- qu.7sfDLA0XcrOfgBR.DeNNs.Xv3reJLBDcqLysIpEHAIpXfFydorTaxI9Ha 8, 38 -- J45t08PcvZcniMffJ4Hs7U4BEzebvsztRo4r1j1fMJ.MjS6kLf1CCMm4J4Kc 8, 38 -- b23q96ZviEci3RnWccXRqY_ivvRawq04X6bt_Sd3bsWuaYChLx3hIxqWEd_q 8, 38 -- tawzp3S4P1pzriPXx9dS2FugyuCgS_ETYx4kl8c2WbyDMHwfCYya72ErVjEc 8, 38 -- qICG9pecv2dZrfqnx_UEw64syZiM3zBdVT9ZIsNsx283b.9Ab8O3p.Do1oTS 8, 38 -- BMLT885uDnbRR84xHRgSLNiB73RTNhqkU0TFPgXQE381RPVF2UyfAjgI3Hs0 8, 38 -- sdrO6QVemrjiNlIS7fwHPlMayyfYLw_nTOXg8EuLDvSgixNcaTU6wKPzNZHZ 8, 38 -- eeodheQVLuWWxQII0AbmVwQEIFC4BekwR2RnACqRrp8nPGLttyjgLAfV_Uc9 8, 38 -- v77KIBklz1PyCRwWd7Xf7yNxKR.f7xmHBybcFr78uUcJIJWhFCSHQavkrjO0 8, 38 -- qzTRGDEgVE2e31hAFXU5Edq9yQRnxjXhM7DCVSFLXWELPdw0tQ9z2T3FL 8, 38 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 8, 38 -- Received: from [192.168.2.3] (vray-at-75.68.231.25 with plain) 8, 38 -- by smtp104.biz.mail.bf1.yahoo.com with SMTP; 31 Aug 2011 15:06:56 -0700 PDT 8, 38 -- Message-ID: {4E5EB082.9050607-at-partbeamsystech.com} 8, 38 -- Date: Wed, 31 Aug 2011 18:06:58 -0400 8, 38 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 8, 38 -- Reply-To: vray-at-partbeamsystech.com 8, 38 -- Organization: PBS&T 8, 38 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0) Gecko/20110812 Thunderbird/6.0 8, 38 -- MIME-Version: 1.0 8, 38 -- To: Annie.Muske-Dukes-Driggs-at-bendresearch.com 8, 38 -- CC: microscopy-at-microscopy.com 8, 38 -- Subject: Re: [Microscopy] [Filtered] HEPA filter system for sputter coater 8, 38 -- References: {201108312126.p7VLQuKG023519-at-ns.microscopy.com} 8, 38 -- In-Reply-To: {201108312126.p7VLQuKG023519-at-ns.microscopy.com} 8, 38 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Here is a link to look at, we use Bancroft and one of my colleagues purchased the flash cards as a useful tool for the lab. There is nothing like a good colour image to compare with.
Hope this helps, Naomi McCallum
Pathology Queensland, Australia.
} } } {microscopylistserver-noreply-at-microscopy.com} 8/31/2011 7:48 am } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpflugheber-at-stlawu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I'm beginning preparations for a new class, and I would like to preview some histology textbooks. The class will be for undergraduates, probably at the 200 level, and will be a combination of histology/methods and microscopy. Does anyone have any favorites that they would recommend I preview? Thanks very much.
==============================Original Headers============================== 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Aug 30 16:37:31 2011 9, 25 -- Received: from znl.com ([206.69.208.20]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7ULbV6V025091 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 30 Aug 2011 16:37:31 -0500 9, 25 -- Received: from localhost (localhost [127.0.0.1]) 9, 25 -- by znl.com (Postfix) with ESMTP id 807B3403E97 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 30 Aug 2011 16:37:31 -0500 (CDT) 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 25 -- Received: from znl.com ([127.0.0.1]) 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 25 -- with ESMTP id R0gd7-hyvbrs for {microscopy-at-microscopy.com} ; 9, 25 -- Tue, 30 Aug 2011 16:37:31 -0500 (CDT) 9, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) 9, 25 -- by znl.com (Postfix) with ESMTPA id 843E3403E8C 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 30 Aug 2011 16:37:30 -0500 (CDT) 9, 25 -- Message-ID: {4E5D5817.4020503-at-microscopy.com} 9, 25 -- Date: Tue, 30 Aug 2011 23:37:27 +0200 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 9, 25 -- Reply-To: jpflugheber-at-stlawu.edu 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 25 -- MIME-Version: 1.0 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 25 -- Subject: viaWWW:Histology textbook recommendation 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 23, 27 -- From prvs=1224c41992=Naomi_McCallum-at-health.qld.gov.au Wed Aug 31 18:49:40 2011 23, 27 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 23, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VNncXl012192 23, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 18:49:39 -0500 23, 27 -- Received: from gwd-mail06.remote.health.qld.gov.au (gwd-mail06.remote.health.qld.gov.au [192.168.3.53]) 23, 27 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id p7VNnMJM008260 23, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:49:22 +1000 23, 27 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 23, 27 -- gwd-mail06.remote.health.qld.gov.au (Queensland Health SMTP Server) 23, 27 -- with ESMTP id 23, 27 -- {T9f30cd07a4c0a803351d0-at-gwd-mail06.remote.health.qld.gov.au} for 23, 27 -- {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:49:03 +1000 23, 27 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 23, 27 -- Novell_GroupWise; Thu, 01 Sep 2011 09:49:02 +1000 23, 27 -- Message-Id: {4E5F550B.88BE.00AA.1-at-health.qld.gov.au} 23, 27 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 23, 27 -- Date: Thu, 01 Sep 2011 09:49:01 +1000 23, 27 -- From: "Naomi McCallum" {Naomi_McCallum-at-health.qld.gov.au} 23, 27 -- To: {Microscopy-at-microscopy.com} 23, 27 -- Subject: Re: [Microscopy] viaWWW:Histology textbook recommendation 23, 27 -- References: {201108302148.p7ULmSrt014453-at-ns.microscopy.com} 23, 27 -- In-Reply-To: {201108302148.p7ULmSrt014453-at-ns.microscopy.com} 23, 27 -- Mime-Version: 1.0 23, 27 -- Content-Type: text/plain; charset="us-ascii" 23, 27 -- Content-Disposition: inline 23, 27 -- Content-Transfer-Encoding: 8bit 23, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p7VNncXl012192 ==============================End of - Headers==============================
If your coater uses a rotary vane oil rough pump, those powders shouldn't be making it out of the exhaust of the pump. They should be trapped in the oil. If the rough pump is a dry pump then the particles are probably being exhausted.
If you really want to trap the particles upstream of the rough pump with a HEPA try Flanders (http://www.flanderscorp.com). I believe they make custom sizes.
Mike
******************************************************************** Michael M. Cheatham 321 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html
On 8/31/11 6:13 PM, "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 16, 34 -- From mmcheath-at-syr.edu Thu Sep 1 06:19:54 2011 16, 34 -- Received: from smtp2.syr.edu (smtp2.syr.edu [128.230.18.92]) 16, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81BJrFl006996 16, 34 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 06:19:54 -0500 16, 34 -- Received: from suex07-hub-02.ad.syr.edu (suex07-hub-02.ad.syr.edu [128.230.108.196]) 16, 34 -- by smtp2.syr.edu (8.14.3/8.14.3) with ESMTP id p81BJrrN017562 16, 34 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 16, 34 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 07:19:53 -0400 16, 34 -- Received: from suex07-mbx-02.ad.syr.edu ([128.230.108.132]) by 16, 34 -- suex07-hub-02.ad.syr.edu ([128.230.108.196]) with mapi; Thu, 1 Sep 2011 16, 34 -- 07:19:52 -0400 16, 34 -- From: Michael M Cheatham {mmcheath-at-syr.edu} 16, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 16, 34 -- Date: Thu, 1 Sep 2011 07:19:50 -0400 16, 34 -- Subject: Re: [Microscopy] Re: [Filtered] HEPA filter system for sputter 16, 34 -- coater 16, 34 -- Thread-Topic: [Microscopy] Re: [Filtered] HEPA filter system for sputter 16, 34 -- coater 16, 34 -- Thread-Index: AcxomRE/Z2kyTk7VTnehRNTocVte3g== 16, 34 -- Message-ID: {CA84E139.3071E%mmcheath-at-syr.edu} 16, 34 -- In-Reply-To: {201108312213.p7VMDQND002618-at-ns.microscopy.com} 16, 34 -- Accept-Language: en-US 16, 34 -- Content-Language: en-US 16, 34 -- X-MS-Has-Attach: 16, 34 -- X-MS-TNEF-Correlator: 16, 34 -- user-agent: Microsoft-MacOutlook/14.12.0.110505 16, 34 -- acceptlanguage: en-US 16, 34 -- Content-Type: text/plain; charset="us-ascii" 16, 34 -- MIME-Version: 1.0 16, 34 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 16, 34 -- definitions=2011-09-01_03:2011-09-01,2011-09-01,1970-01-01 signatures=0 16, 34 -- X-Proofpoint-Spam-Reason: safe 16, 34 -- Content-Transfer-Encoding: 8bit 16, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81BJrFl006996 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dam-at-fzu.cz as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I would like to prepare TEM samples from almost pure tantalum by electropolishing but I´m not sure about the conditions. I have found someone used an electrolyte of sulphupic (5%) and hydrofluoric (2%) acid in methanol. Does anyone have experience with this material? What electrolyte composition, voltage, sample thickness and temperature do you use?
Any help will be very appreciated. Thanks regards
Karel
Karel Dám, MSc Department of Physics AS CR, v. v. i. Na Slovance 2 CZ-182 21 Prague 8 Czech Republic Email: dam-at-fzu.cz
Please think about what you are requesting - and why. 1. I assume you get a vial of produict, or a bag, or a wheelbarrow - nonetheless, much more than you need to study particles or to do EDS/WDS/XRD etc. Where do you do your specimen prep? Lab bench? Hood? Hood with HEPA filter in exhaust? Glove bag? What are the dimensions of the specimen particles? Viruses are relatively large and HEPA filters are designed to safeguard us from pathogens and allergens. The HEPA standard: http://en.wikipedia.org/wiki/HEPA, as explained in Wikipedia helps us to understand the characteristics of the HEPA filtration systems which we might consider during our search and to increase the chance that we will get what we REALLY want. However, the acid test is whether we KNOW the properties of the smallest particles we must trap and what hazard they present. 2. Why are you worried about your sputter coater? The great majority of particles will be trapped in the oil of your vacuum pump - sputter coater, (E)SEM and little will get by the mist filter on the pump. If you are using a dry pump, then, are you worried about contaminating subsequent specimens, the chamber of the coater, or the room?
The two links at the top of the page should direct you in the right direction for HEPA filter consumables and systems. Remember, you can insert a 'filter' for almost anything between the chamber and the pump, but will you still be able to pull the vacuum with your current vacuum pump?
As is almost always the case with microscopy, we must adapt a little or adapt a lot, and most of us have to learn where and when we stop considering cost as a limitation.
In woodworking, for example, if you have a child who is about to take wood shop, wouldn't you like to know about this: http://www.sawstop.com/?gclid=CK-3zMOZ_KoCFQ0BQAod-UZo2g?
Hope this helps,
Fred Monson http://cmirt.wcupa.edu CMIRT, West Chester University of Pennsylvania West Chester, PA, 19383 610-738-0437 ________________________________________
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi everyone,
My lab works with pharmaceutical powders and we almost always perform some SEM on them. While the powders are technically stuck to the posts with adhesive I assume some loose powder is sucked up through the vacuum system while sputter coating. It appears to me that the sputter coater has no filtration system of any kind and could technically vent any powders back into the lab through the exhaust. It's an older Hummer 6.2 system (no digital display so I assume it's at least 10 years old), the exhaust port we want to cover is 6inchesx6inches.
I'd like to put a HEPA filter on the exhaust port but I contacted Anatech and they didn't know a supplier for a housing like this. Most HEPA filter housings I'm finding online are way too large for the exhaust port, has anyone does this? Did you make your own filter housing or is there a supplier for this kind of thing?
Thanks,
Annie
Disclaimer - August 31, 2011 LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling Bend Research Inc (541) 382-4100.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:25:56 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLPuKx022026 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id A25B9404A8A 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id TEfrgTipHaWS for {microscopy-at-microscopy.com} ; 12, 25 -- Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) 12, 25 -- by znl.com (Postfix) with ESMTPA id B2D76404A7F 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:55 -0500 (CDT) 12, 25 -- Message-ID: {4E5EA6E1.8020005-at-microscopy.com} 12, 25 -- Date: Wed, 31 Aug 2011 23:25:53 +0200 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 -- Subject: [Filtered] HEPA filter system for sputter coater 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Recipes for electropolishing tantalum from Electron Microscopy of Thin Crystals by PB Hirsch et al.:
1) Electrolyte: 10% HF (40% concentration) in 90% sulphuric acid. Voltage: 12-20V Current Density: 1-2 A/cm2 Temperature: room temp. Pt or C cathode
2) Electrolyte: 5% sulphuric acid, 1.25% HF (40% concentration), and 93.75% methanol. Voltage: 50-70V Current Density: 4.4 A/cm2 Temperature: 0C (ice water bath) Pt or C cathode
Similar recipes for electropolishing Ta from Practical Electron Microscopy in Materials Science by KC Thompson-Russell and JW Edington:
3) Electrolyte: 600ml methanol, 30ml sulphuric acid, and 7.5ml HF. Voltage: 20V Current Density: 26-28 A/cm2 (seems very high--be careful) Temperature: room temp. wash in ethanol
4) Electrolyte: 90% sulphuric acid and 10% HF. Voltage: 14V Current Density: 0.1-0.2 A/cm2 Temperature: room temp. Pt cathode only wash in water
Make sure you have a fresh supply of calcium gluconate gel on hand when working with HF-based electrolytes!
Good luck, Chris
On Thu, Sep 1, 2011 at 10:11 AM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both dam-at-fzu.cz as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: dam-at-fzu.cz Name: Karel Dám } } Title-Subject: [Filtered] Tantalum electropolishing } } Message: Dear listers, } } I would like to prepare TEM samples from almost pure tantalum by } electropolishing but I´m not sure about the conditions. I have found } someone used an electrolyte of sulphupic (5%) and hydrofluoric (2%) acid } in methanol. Does anyone have experience with this material? What } electrolyte composition, voltage, sample thickness and temperature do } you use? } } Any help will be very appreciated. } Thanks } regards } } Karel } } Karel Dám, MSc } Department of Physics AS CR, v. v. i. } Na Slovance 2 } CZ-182 21 Prague 8 } Czech Republic } Email: dam-at-fzu.cz } } Login Host: 147.231.26.31 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Sep 1 09:02:24 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81E2Oes025496 } 11, 25 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:24 -0500 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id AAEB24052F2 } 11, 25 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:24 -0500 (CDT) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 25 -- with ESMTP id 2TtPe9ksWBS0 for {microscopy-at-microscopy.com} ; } 11, 25 -- Thu, 1 Sep 2011 09:02:24 -0500 (CDT) } 11, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id 63F5B4052E2 } 11, 25 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:23 -0500 (CDT) } 11, 25 -- Message-ID: {4E5F906C.8050003-at-microscopy.com} } 11, 25 -- Date: Thu, 01 Sep 2011 16:02:20 +0200 } 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 11, 25 -- Reply-To: dam-at-fzu.cz } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: viaWWW: Tantalum electropolishing } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
-- Christopher Winkler Dynamic Characterization Group http://www.materials.drexel.edu/dcg/ Drexel University 267-496-0587
==============================Original Headers============================== 14, 28 -- From microwink-at-gmail.com Thu Sep 1 09:53:04 2011 14, 28 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 14, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81Er37w026513 14, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:53:03 -0500 14, 28 -- Received: by bkbzt4 with SMTP id zt4so1985551bkb.0 14, 28 -- for {Microscopy-at-microscopy.com} ; Thu, 01 Sep 2011 07:53:03 -0700 (PDT) 14, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 28 -- d=gmail.com; s=gamma; 14, 28 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 14, 28 -- :content-type:content-transfer-encoding; 14, 28 -- bh=L9YGYDrhejVilZdIDx8X8AXItPQMN5EIaPzw5Ry5lIA=; 14, 28 -- b=w8bQLXDRYlH7KSOoFvfZZkbM/dCBXwoirkJsDZFkJlLTODdq84M6U1Gksvo2BXaZFu 14, 28 -- kNhpNu596QhQhvtg0FerO8UgztwYR+XWklU0f7HG+DinP4HWoqeDzv1+BdgmE9Yf+GZJ 14, 28 -- 8Wu7c9hZwv57J3mHNzrFZWHP/Ve+XlWYevnUA= 14, 28 -- MIME-Version: 1.0 14, 28 -- Received: by 10.204.135.25 with SMTP id l25mr1056971bkt.200.1314888781266; 14, 28 -- Thu, 01 Sep 2011 07:53:01 -0700 (PDT) 14, 28 -- Received: by 10.204.24.144 with HTTP; Thu, 1 Sep 2011 07:53:01 -0700 (PDT) 14, 28 -- In-Reply-To: {201109011411.p81EBXhx015067-at-ns.microscopy.com} 14, 28 -- References: {201109011411.p81EBXhx015067-at-ns.microscopy.com} 14, 28 -- Date: Thu, 1 Sep 2011 10:53:01 -0400 14, 28 -- Message-ID: {CAA8T2PPmpwrMQ5uaFEYh3efjLKiENRy+RTWf45wkrs3nTMGGPg-at-mail.gmail.com} 14, 28 -- Subject: Re: [Microscopy] viaWWW: Tantalum electropolishing 14, 28 -- From: Christopher Winkler {microwink-at-gmail.com} 14, 28 -- To: Microscopy-at-microscopy.com 14, 28 -- Content-Type: text/plain; charset=ISO-8859-1 14, 28 -- Content-Transfer-Encoding: 8bit 14, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81Er37w026513 ==============================End of - Headers==============================
Does anyone have a good protocol or know a reference for digesting arthropod cuticle with chitinase?
Thanks,
Neeraj.
Neeraj V. Gohad, Ph.D. Research Assistant Professor Department of Biological Sciences 132 Long Hall Clemson University Clemson,SC-29634 Phone: 864-656-3597 Fax: 864-656-0435
we normally use a light puff of air from a simple dust-off (air blower) to make sure there are no loose particles on our SEM stubs once they are stuck to the stub. If the samples are hazardous this would be done inside of an enclosure such as a fume hood or other extraction system. This would perhaps need more regular cleaning if you prepared many samples.
It would be relatively simple to trap most particles by partially enclosing the process or alternatively remove loose particles with a HEPA filtered vacuum cleaner.
Malcolm
Malcolm Haswell Imaging Suite Faculty of Applied Sciences University of Sunderland SUNDERLAND SR1 3SD UK email: malcolm.haswell-at-sunderland.ac.uk
________________________________________ X-from: FMonson-at-wcupa.edu [FMonson-at-wcupa.edu] Sent: 01 September 2011 15:15 To: Malcolm Haswell
Please think about what you are requesting - and why. 1. I assume you get a vial of produict, or a bag, or a wheelbarrow - nonetheless, much more than you need to study particles or to do EDS/WDS/XRD etc. Where do you do your specimen prep? Lab bench? Hood? Hood with HEPA filter in exhaust? Glove bag? What are the dimensions of the specimen particles? Viruses are relatively large and HEPA filters are designed to safeguard us from pathogens and allergens. The HEPA standard: http://en.wikipedia.org/wiki/HEPA, as explained in Wikipedia helps us to understand the characteristics of the HEPA filtration systems which we might consider during our search and to increase the chance that we will get what we REALLY want. However, the acid test is whether we KNOW the properties of the smallest particles we must trap and what hazard they present. 2. Why are you worried about your sputter coater? The great majority of particles will be trapped in the oil of your vacuum pump - sputter coater, (E)SEM and little will get by the mist filter on the pump. If you are using a dry pump, then, are you worried about contaminating subsequent specimens, the chamber of the coater, or the room?
The two links at the top of the page should direct you in the right direction for HEPA filter consumables and systems. Remember, you can insert a 'filter' for almost anything between the chamber and the pump, but will you still be able to pull the vacuum with your current vacuum pump?
As is almost always the case with microscopy, we must adapt a little or adapt a lot, and most of us have to learn where and when we stop considering cost as a limitation.
In woodworking, for example, if you have a child who is about to take wood shop, wouldn't you like to know about this: http://www.sawstop.com/?gclid=CK-3zMOZ_KoCFQ0BQAod-UZo2g?
Hope this helps,
Fred Monson http://cmirt.wcupa.edu CMIRT, West Chester University of Pennsylvania West Chester, PA, 19383 610-738-0437 ________________________________________
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi everyone,
My lab works with pharmaceutical powders and we almost always perform some SEM on them. While the powders are technically stuck to the posts with adhesive I assume some loose powder is sucked up through the vacuum system while sputter coating. It appears to me that the sputter coater has no filtration system of any kind and could technically vent any powders back into the lab through the exhaust. It's an older Hummer 6.2 system (no digital display so I assume it's at least 10 years old), the exhaust port we want to cover is 6inchesx6inches.
I'd like to put a HEPA filter on the exhaust port but I contacted Anatech and they didn't know a supplier for a housing like this. Most HEPA filter housings I'm finding online are way too large for the exhaust port, has anyone does this? Did you make your own filter housing or is there a supplier for this kind of thing?
Thanks,
Annie
Disclaimer - August 31, 2011 LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling Bend Research Inc (541) 382-4100.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:25:56 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLPuKx022026 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id A25B9404A8A 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id TEfrgTipHaWS for {microscopy-at-microscopy.com} ; 12, 25 -- Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) 12, 25 -- by znl.com (Postfix) with ESMTPA id B2D76404A7F 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:55 -0500 (CDT) 12, 25 -- Message-ID: {4E5EA6E1.8020005-at-microscopy.com} 12, 25 -- Date: Wed, 31 Aug 2011 23:25:53 +0200 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 -- Subject: [Filtered] HEPA filter system for sputter coater 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 25, 38 -- From FMonson-at-wcupa.edu Thu Sep 1 09:02:46 2011 25, 38 -- Received: from MX01.WCUPA.EDU (mx01.wcupa.edu [144.26.117.250]) 25, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81E2jg0025964 25, 38 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:46 -0500 25, 38 -- X-ASG-Debug-ID: 1314885765-0368bc42ad7e830001-4CH8be 25, 38 -- Received: from WCU-XCH-09.PASSHE.LCL (wcu-xch-09.wcupa.edu [144.26.0.93]) by MX01.WCUPA.EDU with ESMTP id 9bgJfaID4F0wokxz (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Thu, 01 Sep 2011 10:02:45 -0400 (EDT) 25, 38 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 25, 38 -- X-Barracuda-Apparent-Source-IP: 144.26.0.93 25, 38 -- X-ASG-Whitelist: Client 25, 38 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 25, 38 -- WCU-XCH-09.PASSHE.LCL ([fe80::ec79:d7c5:7b66:96e5%16]) with mapi id 25, 38 -- 14.01.0270.001; Thu, 1 Sep 2011 10:02:44 -0400 25, 38 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 25, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 25, 38 -- CC: "Annie.Muske-Dukes-Driggs-at-bendresearch.com" 25, 38 -- {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 25, 38 -- Subject: RE: [Microscopy] [Filtered] HEPA filter system for sputter coater 25, 38 -- Thread-Topic: [Microscopy] [Filtered] HEPA filter system for sputter coater 25, 38 -- X-ASG-Orig-Subj: RE: [Microscopy] [Filtered] HEPA filter system for sputter coater 25, 38 -- Thread-Index: AQHMaCYr40j9PWWa3UWVS/MHpyCJRpU4iE8v 25, 38 -- Date: Thu, 1 Sep 2011 14:02:44 +0000 25, 38 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C0ABF22-at-WCU-XCH-03.PASSHE.LCL} 25, 38 -- References: {201108312137.p7VLbJsr015785-at-ns.microscopy.com} 25, 38 -- In-Reply-To: {201108312137.p7VLbJsr015785-at-ns.microscopy.com} 25, 38 -- Accept-Language: en-US 25, 38 -- Content-Language: en-US 25, 38 -- X-MS-Has-Attach: 25, 38 -- X-MS-TNEF-Correlator: 25, 38 -- x-originating-ip: [10.32.20.205] 25, 38 -- Content-Type: text/plain; charset="us-ascii" 25, 38 -- MIME-Version: 1.0 25, 38 -- X-Barracuda-Connect: wcu-xch-09.wcupa.edu[144.26.0.93] 25, 38 -- X-Barracuda-Start-Time: 1314885765 25, 38 -- X-Barracuda-Encrypted: AES128-SHA 25, 38 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 25, 38 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 25, 38 -- Content-Transfer-Encoding: 8bit 25, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81E2jg0025964 ==============================End of - Headers==============================Visit www.sunderland.ac.uk/tv to see the University's new TV ads
==============================Original Headers============================== 34, 37 -- From malcolm.haswell-at-sunderland.ac.uk Thu Sep 1 10:20:24 2011 34, 37 -- Received: from max1.sunderland.ac.uk ([157.228.52.120]) 34, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p81FKNAj027272 34, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 10:20:24 -0500 34, 37 -- Received: (qmail 23852 invoked from network); 1 Sep 2011 15:14:44 -0000 34, 37 -- Received: from localhost (127.0.0.1) 34, 37 -- by max1.sunderland.ac.uk with SMTP; 1 Sep 2011 15:14:44 -0000 34, 37 -- Received: from max1.sunderland.ac.uk ([127.0.0.1]) 34, 37 -- by localhost (max1.sunderland.ac.uk [127.0.0.1]) (amavisd-new, port 10024) 34, 37 -- with SMTP id 21610-10 for {Microscopy-at-microscopy.com} ; 34, 37 -- Thu, 1 Sep 2011 16:14:12 +0100 (BST) 34, 37 -- Received: (qmail 21163 invoked by uid 607); 1 Sep 2011 15:08:14 -0000 34, 37 -- Received: from unknown (HELO cas-01.uni.ad.sunderland.ac.uk) (157.228.67.41) 34, 37 -- by max1.sunderland.ac.uk (qpsmtpd/0.28) with ESMTP; Thu, 01 Sep 2011 16:08:14 +0100 34, 37 -- Received: from MB-01.uni.ad.sunderland.ac.uk ([169.254.1.36]) by 34, 37 -- cas-01.uni.ad.sunderland.ac.uk ([157.228.67.41]) with mapi; Thu, 1 Sep 2011 34, 37 -- 16:12:36 +0100 34, 37 -- From: Malcolm Haswell {malcolm.haswell-at-sunderland.ac.uk} 34, 37 -- To: "Microscopy MSA (Microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com} 34, 37 -- Subject: RE: [Microscopy] RE: [Filtered] HEPA filter system for sputter 34, 37 -- coater 34, 37 -- Thread-Topic: [Microscopy] RE: [Filtered] HEPA filter system for sputter 34, 37 -- coater 34, 37 -- Thread-Index: AQHMaLGPK5IUJIMjwkWMHCVKG+d3gpU4nUtW 34, 37 -- Date: Thu, 1 Sep 2011 15:12:36 +0000 34, 37 -- Message-ID: {F258EBB933D8F549902DDFC16829705D148FE0A9-at-mb-01.uni.ad.sunderland.ac.uk} 34, 37 -- References: {201109011415.p81EF6bs025560-at-ns.microscopy.com} 34, 37 -- In-Reply-To: {201109011415.p81EF6bs025560-at-ns.microscopy.com} 34, 37 -- Accept-Language: en-GB, en-US 34, 37 -- Content-Language: en-GB 34, 37 -- X-MS-Has-Attach: 34, 37 -- X-MS-TNEF-Correlator: 34, 37 -- Content-Type: text/plain; charset="us-ascii" 34, 37 -- MIME-Version: 1.0 34, 37 -- X-Virus-Scanned: by iCritical at max1.sunderland.ac.uk 34, 37 -- Content-Transfer-Encoding: 8bit 34, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81FKNAj027272 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jjmccarthy-at-wisc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jjmccarthy-at-wisc.edu Name: Jon McCarthy
Organization: College of Engineering: UW Madison
Title-Subject: [Filtered] JEOL JBX-5DII lithography systems
Message: We have two 1995 vintage JBX instruments we wish to fiind a home for. On system is used for parts to support the other. Neither system has a warking computer, but we are getting a quote for a working VAX for the system that was useable before shutdown in 2010.
We can supply specifications to interested folks. We will pallatize and shrink rap for pickup, you pay shipping. We will need about $10000 to cover our costs to disassemble and pack. We can negotiate. Final Sale would be through the UW SWAP oranganization.
Contact; Jon J McCarthy, Ph.D. Director, Shared Instrument Facilities Co-Director Advance Materials Industrial Consortium UW College of Engineering Phone: 608-263-1073 Cell:608-345-6134 jjmccarthy-at-wisc.edu
For a bit of background: prepping samples in my lab almost always requires a respirator/filtered mask or in rare cases a suit with its own air supply and anyone else in the room has to wear a mask while sample prep is going on. The procedure has been to prep samples the in a HEPA lab hood with a mask and transfer them to the sputter coater (which is just on a bench and not in a hood). As soon as the posts are in the sputter coater people considered samples contained and would remove their masks. We also consider the samples contained after coating so people do not wear masks to transfer samples to the SEM or for imaging.
During a safety check of the lab the officers were concerned that over the years powder has been blown back into the lab and since I wasn't able to show them any filter system they wanted me to look into adding one. The particles in the samples vary in size from {10µm to } 200µm, I will look into the oil filters and find out if they catch something of that size. If not you have all given me a lot of options.
Thanks again, Annie
-----Original Message----- X-from: Monson, Frederick [mailto:FMonson-at-wcupa.edu] Sent: Thursday, September 01, 2011 7:03 AM To: microscopy-at-microscopy.com Cc: Muske-Dukes-Driggs, Annie
Hi everyone,
My lab works with pharmaceutical powders and we almost always perform some SEM on them. While the powders are technically stuck to the posts with adhesive I assume some loose powder is sucked up through the vacuum system while sputter coating. It appears to me that the sputter coater has no filtration system of any kind and could technically vent any powders back into the lab through the exhaust. It's an older Hummer 6.2 system (no digital display so I assume it's at least 10 years old), the exhaust port we want to cover is 6inchesx6inches.
I'd like to put a HEPA filter on the exhaust port but I contacted Anatech and they didn't know a supplier for a housing like this. Most HEPA filter housings I'm finding online are way too large for the exhaust port, has anyone does this? Did you make your own filter housing or is there a supplier for this kind of thing?
Thanks,
Annie
Disclaimer - August 31, 2011 LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling Bend Research Inc (541) 382-4100.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:25:56 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLPuKx022026 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id A25B9404A8A 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id TEfrgTipHaWS for {microscopy-at-microscopy.com} ; 12, 25 -- Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) 12, 25 -- by znl.com (Postfix) with ESMTPA id B2D76404A7F 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:55 -0500 (CDT) 12, 25 -- Message-ID: {4E5EA6E1.8020005-at-microscopy.com} 12, 25 -- Date: Wed, 31 Aug 2011 23:25:53 +0200 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 -- Subject: [Filtered] HEPA filter system for sputter coater 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 30, 28 -- From Annie.Muske-Dukes-Driggs-at-bendresearch.com Thu Sep 1 12:26:52 2011 30, 28 -- Received: from smtp.bendresearch.com (smtp.bendres.com [67.59.84.113]) 30, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81HQpcX029190 30, 28 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 12:26:51 -0500 30, 28 -- Received: from mail pickup service by smtp.bendresearch.com with Microsoft SMTPSVC; 30, 28 -- Thu, 1 Sep 2011 10:26:13 -0700 30, 28 -- Importance: normal 30, 28 -- Priority: normal 30, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.3790.4862 30, 28 -- MIME-Version: 1.0 30, 28 -- Content-Type: text/plain; 30, 28 -- charset="iso-8859-1" 30, 28 -- Content-Class: urn:content-classes:message 30, 28 -- x-vipre-scanned: 0FB71ACB0028260FB71C18 30, 28 -- Subject: RE: [Microscopy] [Filtered] HEPA filter system for sputter coater 30, 28 -- Date: Thu, 1 Sep 2011 10:26:12 -0700 30, 28 -- Message-ID: {82C755170EE5C44BA26E35A7F6B3864A01EB01DA-at-dixie.bri.local} 30, 28 -- In-Reply-To: {899EAA40AE6E47488CE0637489EFB30C0ABF22-at-WCU-XCH-03.PASSHE.LCL} 30, 28 -- X-MS-Has-Attach: 30, 28 -- X-MS-TNEF-Correlator: 30, 28 -- Thread-Topic: [Microscopy] [Filtered] HEPA filter system for sputter coater 30, 28 -- thread-index: AQHMaCYr40j9PWWa3UWVS/MHpyCJRpU4iE8vgAA4iSA= 30, 28 -- References: {201108312137.p7VLbJsr015785-at-ns.microscopy.com} {899EAA40AE6E47488CE0637489EFB30C0ABF22-at-WCU-XCH-03.PASSHE.LCL} 30, 28 -- From: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 30, 28 -- To: {microscopy-at-microscopy.com} 30, 28 -- X-OriginalArrivalTime: 01 Sep 2011 17:26:13.0809 (UTC) FILETIME=[3FB11A10:01CC68CC] 30, 28 -- Content-Transfer-Encoding: 8bit 30, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81HQpcX029190 ==============================End of - Headers==============================
Back in the late 60's a British company called AEI manufactured a TEM with two WDS spectrometers. It was called EMMA-4. It was not a huge commercial success, and no one has tried to make a microscope like that since.
John Mardinly ASU } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both prlax-at-mindspring.com as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: prlax-at-mindspring.com Name: David OHara } } Organization: Parallax Research, Inc. } } Title-Subject: [Filtered] WDS capabilities } } Message: First, I want to thank Dr Peter Ingram for reminding me of this } listServer. } Next, my primary interest is in developing new WDS type instrumentation } so I like to see questions concerning WDS and things it can do and } potential applications. For example, there was a thread a couple of } months ago concerning measuring Na and Zn and someone asked if WDS could } resolve this overlap. The answer is "Yes" if the right diffractor is } used (TAP will work but the multilayer diffractors will still give an } overlap) } Not being an EM trained person, the needs for WDS are often not obvious } to me so this forum will be especially valueable. } So I have a few questions for EM users: } 1: Is anybody interested in measuring Li in their SEM? } 2: I was told by somebody that the Li line at 52 eV was due to a } "forbidden" transition so we would never see it....correct? } 3: Why do I never hear about WDS on TEMs? } } Thank You, } } David OHara } } } Login Host: 68.35.253.50 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:28:13 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLSDkb025337 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:28:13 -0500 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id 34A0D404A9B } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:28:13 -0500 (CDT) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 11, 25 -- with ESMTP id XCFlhlWpG9Q6 for {microscopy-at-microscopy.com} ; } 11, 25 -- Wed, 31 Aug 2011 16:28:12 -0500 (CDT) } 11, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id AED5B404A90 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:28:11 -0500 (CDT) } 11, 25 -- Message-ID: {4E5EA768.9090807-at-microscopy.com} } 11, 25 -- Date: Wed, 31 Aug 2011 23:28:08 +0200 } 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 11, 25 -- Reply-To: prlax-at-mindspring.com } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: [Filtered] MicroscopyListserverviaWWW: } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 3, 39 -- From John.Mardinly-at-asu.edu Thu Sep 1 13:58:27 2011 3, 39 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 3, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81IwRE5014292 3, 39 -- for {Microscopy-at-Microscopy.com} ; Thu, 1 Sep 2011 13:58:27 -0500 3, 39 -- X-ASG-Debug-ID: 1314903302-03efbe224100090015-FOsErg 3, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id eLX1Wcex1jjk1G6V (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO) for {Microscopy-at-Microscopy.com} ; Thu, 01 Sep 2011 11:56:42 -0700 (MST) 3, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 3, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 3, 39 -- X-ASG-Whitelist: Client 3, 39 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.206) by 3, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 3, 39 -- 8.2.255.0; Thu, 1 Sep 2011 11:54:27 -0700 3, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by 3, 39 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 1 Sep 3, 39 -- 2011 11:54:27 -0700 3, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 3, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Thu, 1 Sep 2011 3, 39 -- 18:54:27 +0000 3, 39 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: 3, 39 -- References: {201108312139.p7VLdsNR022099-at-ns.microscopy.com} 3, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW: 3, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} 3, 39 -- thread-topic: [Microscopy] [Filtered] MicroscopyListserverviaWWW: 3, 39 -- thread-index: Acxo2JKrFT1xRG04RrqvbcCfWVf6fw== 3, 39 -- Content-Type: text/plain; charset="us-ascii" 3, 39 -- In-Reply-To: {201108312139.p7VLdsNR022099-at-ns.microscopy.com} 3, 39 -- Message-ID: {1E17DF9A-722D-459B-A437-815CE9AE8AFD-at-exchange.asu.edu} 3, 39 -- Date: Thu, 1 Sep 2011 11:58:29 -0700 3, 39 -- To: MSA {Microscopy-at-Microscopy.com} 3, 39 -- CC: {John.Mardinly-at-asu.edu} 3, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) 3, 39 -- X-OriginalArrivalTime: 01 Sep 2011 18:54:27.0187 (UTC) FILETIME=[92CA7C30:01CC68D8] 3, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 3, 39 -- X-Barracuda-Start-Time: 1314903402 3, 39 -- X-Barracuda-Encrypted: RC4-MD5 3, 39 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 3, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu 3, 39 -- Content-Transfer-Encoding: 8bit 3, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81IwRE5014292 ==============================End of - Headers==============================
A technical position is available in the Microscopy Facility of the Department of Anatomy and Neurobiology in the School of Medicine at Virginia Commonwealth University. The facility houses confocal, multi-photon, TIRF, atomic force, and electron microscopes (TEM & SEM). The successful candidate will assist with microscopy studies of various biological systems. Duties include assisting users of the facility, providing basic instruction in the use of equipment within the facility (i.e. microscopes, microtomes, and image analysis programs), sample preparation, minor equipment maintenance and some administrative work (ordering of supplies and monthly billing). Applicants should have excellent communication and organizational skills, an understanding of laboratory procedures, and the ability to manage a large and varied workload. Minimum qualifications include a bachelor’s degree in Science (with a concentration in Biology), at least 2 years of hands-on experience with advanced light microscopy (e.g. confocal), electron microscopy, sample preparation (including sectioning), and image analysis. Computer skills are essential.
To apply, go to the VCU Jobs website at: https://www.vcujobs.com/
Click the Search Postings link in the upper left corner of the window. The position number is 551220.
--------------------------
Scott Henderson, Ph.D. Director of Microscopy Associate Professor Dept. Anatomy & Neurobiology Virginia Commonwealth University School of Medicine 1101 East Marshall St. Richmond, VA 23298-0709
==============================Original Headers============================== 9, 30 -- From schenderson-at-vcu.edu Thu Sep 1 15:26:54 2011 9, 30 -- Received: from ironport2.ucc.vcu.edu (ironport2.ucc.vcu.edu [128.172.8.176]) 9, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p81KQsX6031506 9, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 15:26:54 -0500 9, 30 -- X-IronPort-AV: E=Sophos;i="4.68,315,1312171200"; 9, 30 -- d="scan'208";a="109024288" 9, 30 -- Received: from cedar.vcu.edu ([128.172.8.172]) 9, 30 -- by ironport2out.ucc.vcu.edu with ESMTP; 01 Sep 2011 16:26:58 -0400 9, 30 -- Received: from SOMANSH09053AA ([128.172.29.191]) 9, 30 -- by cedar.vcu.edu (Lotus Domino Release 8.5.1FP5) 9, 30 -- with ESMTP id 2011090116265185-1168799 ; 9, 30 -- Thu, 1 Sep 2011 16:26:51 -0400 9, 30 -- From: "Scott Henderson" {schenderson-at-vcu.edu} 9, 30 -- To: {Microscopy-at-microscopy.com} 9, 30 -- Subject: Microscopy Technician position available 9, 30 -- Date: Thu, 1 Sep 2011 16:26:47 -0400 9, 30 -- Message-ID: {06482017E7E94F1E8DEF6C7A1B91C32B-at-adm.adp.vcu.edu} 9, 30 -- MIME-Version: 1.0 9, 30 -- X-Mailer: Microsoft Office Outlook 11 9, 30 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 9, 30 -- Thread-Index: Acxo5XkuM58vrc8DQDeBR3Y2kYQ01g== 9, 30 -- X-MIMETrack: Itemize by SMTP Server on CEDAR/VCU(Release 8.5.1FP5|September 29, 2010) at 9, 30 -- 09/01/2011 04:26:51 PM, 9, 30 -- Serialize by Router on CEDAR/VCU(Release 8.5.1FP5|September 29, 2010) at 09/01/2011 9, 30 -- 04:26:52 PM, 9, 30 -- Serialize complete at 09/01/2011 04:26:52 PM 9, 30 -- Content-Type: text/plain; 9, 30 -- charset="iso-8859-1" 9, 30 -- Content-Transfer-Encoding: 8bit 9, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p81KQsX6031506 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ekoh-at-udel.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ekoh-at-udel.edu Name: Ed Kohut
Organization: Dept. of Geological Sciences, University of Delaware
Title-Subject: [Filtered] Surplus: carbon coater and X-ray generator
Message: The UD Dept. of Geosciences is surplusing their remaining mineralogical analytical equipment. The following items are available: Denton DV-502 Carbon Coater
Phillips PW1729 X-Ray Generator (no tube or tower)
If interested please contact Bill Parnella at parnella-at-udel.edu or 302-831-3156
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both deanr-at-dickinson.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I have some small corals from a colleague who is interested in seasonal variations of Arg/Cal. Any ideas on how to measure Aragonite/Calcite ratios on a sample powder where there isn't enough material for XRD?
That sounds like a very small amount of powder. You might look into analyzing it with FTIR since there is a difference between the spectra. Good luck!
Diane Ciaburri
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, September 01, 2011 5:22 PM To: Ciaburri, Diane A.
If powders were sucked away, wouldn't they just go into the oil of the vacuum pump?
Ron L
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday, August 31, 2011 5:29 PM To: lherault-at-bu.edu
Hi everyone,
My lab works with pharmaceutical powders and we almost always perform some SEM on them. While the powders are technically stuck to the posts with adhesive I assume some loose powder is sucked up through the vacuum system while sputter coating. It appears to me that the sputter coater has no filtration system of any kind and could technically vent any powders back into the lab through the exhaust. It's an older Hummer 6.2 system (no digital display so I assume it's at least 10 years old), the exhaust port we want to cover is 6inchesx6inches.
I'd like to put a HEPA filter on the exhaust port but I contacted Anatech and they didn't know a supplier for a housing like this. Most HEPA filter housings I'm finding online are way too large for the exhaust port, has anyone does this? Did you make your own filter housing or is there a supplier for this kind of thing?
Thanks,
Annie
Disclaimer - August 31, 2011 LEGAL NOTICE: This message (and/or any attachments accompanying it) is confidential and proprietary. It is intended for the addressee(s) only. Access to this e-mail by anyone else is unauthorized. If you are not an addressee any disclosure, copying, or distribution of the contents of this e-mail (and any attachments) or any action taken (or not taken) in reliance upon this message is unauthorized and may be unlawful. If you are not an addressee, please contact the sender immediately by calling Bend Research Inc (541) 382-4100.
==============================Original Headers============================== 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Aug 31 16:25:56 2011 12, 25 -- Received: from znl.com ([206.69.208.20]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p7VLPuKx022026 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 12, 25 -- Received: from localhost (localhost [127.0.0.1]) 12, 25 -- by znl.com (Postfix) with ESMTP id A25B9404A8A 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 25 -- Received: from znl.com ([127.0.0.1]) 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 25 -- with ESMTP id TEfrgTipHaWS for {microscopy-at-microscopy.com} ; 12, 25 -- Wed, 31 Aug 2011 16:25:56 -0500 (CDT) 12, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) 12, 25 -- by znl.com (Postfix) with ESMTPA id B2D76404A7F 12, 25 -- for {microscopy-at-microscopy.com} ; Wed, 31 Aug 2011 16:25:55 -0500 (CDT) 12, 25 -- Message-ID: {4E5EA6E1.8020005-at-microscopy.com} 12, 25 -- Date: Wed, 31 Aug 2011 23:25:53 +0200 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 25 -- Reply-To: "Muske-Dukes-Driggs, Annie" {Annie.Muske-Dukes-Driggs-at-bendresearch.com} 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 25 -- MIME-Version: 1.0 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 25 -- Subject: [Filtered] HEPA filter system for sputter coater 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 20, 22 -- From lherault-at-bu.edu Thu Sep 1 20:04:26 2011 20, 22 -- Received: from vms173017pub.verizon.net (vms173017pub.verizon.net [206.46.173.17]) 20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8214Qpv002614 20, 22 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 20:04:26 -0500 20, 22 -- Received: from RonLHeraultPC ([unknown] [108.20.114.189]) 20, 22 -- by vms173017.mailsrvcs.net 20, 22 -- (Sun Java(tm) System Messaging Server 7u2-7.02 32bit (built Apr 16 2009)) 20, 22 -- with ESMTPA id {0LQV004WVEZ8FRZ0-at-vms173017.mailsrvcs.net} for 20, 22 -- microscopy-at-microscopy.com; Thu, 01 Sep 2011 20:04:21 -0500 (CDT) 20, 22 -- From: "Ron L'Herault" {lherault-at-bu.edu} 20, 22 -- To: {microscopy-at-microscopy.com} 20, 22 -- References: {201108312129.p7VLTBRM028719-at-ns.microscopy.com} 20, 22 -- In-reply-to: {201108312129.p7VLTBRM028719-at-ns.microscopy.com} 20, 22 -- Subject: RE: [Microscopy] [Filtered] HEPA filter system for sputter coater 20, 22 -- Date: Thu, 01 Sep 2011 21:03:33 -0400 20, 22 -- Message-id: {011101cc690c$233a7760$69af6620$-at-edu} 20, 22 -- MIME-version: 1.0 20, 22 -- Content-type: text/plain; charset=US-ASCII 20, 22 -- Content-transfer-encoding: 7bit 20, 22 -- X-Mailer: Microsoft Office Outlook 12.0 20, 22 -- Thread-index: AcxoJR016mm/3v8ZSKaaxQrPP1PRFAA5pzZg 20, 22 -- Content-language: en-us ==============================End of - Headers==============================
You could try Raman. There are some low wave number peaks that you can use to distinguish between the two polymorphs. Sometime fluorescence is a problem, but you might get lucky. You'd also have to make up some powders of known ratios to use as calibration standards, but after that the actual time for each Raman analysis would be less than a minute. Dan
At 02:30 PM 01/09/2011, you wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 8, 19 -- From marshall-at-sfu.ca Thu Sep 1 22:22:52 2011 8, 19 -- Received: from pobox.sfu.ca (pobox.sfu.ca [142.58.101.28]) 8, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p823MpgP020636 8, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 22:22:51 -0500 8, 19 -- Received: from X201.sfu.ca (S0106001c232b79fb.vn.shawcable.net [174.7.122.17]) 8, 19 -- (authenticated bits=0) 8, 19 -- by pobox.sfu.ca (8.13.6/8.13.5/SFU-6.0G) with ESMTP id p823MnJR011112 8, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT) 8, 19 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 20:22:50 -0700 (PDT) 8, 19 -- Message-Id: {201109020322.p823MnJR011112-at-pobox.sfu.ca} 8, 19 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 8, 19 -- Date: Thu, 01 Sep 2011 20:22:55 -0700 8, 19 -- To: Microscopy-at-microscopy.com 8, 19 -- From: Dan Marshall {marshall-at-sfu.ca} 8, 19 -- Subject: Re: [Microscopy] viaWWW:Measuring Aragonite/Calcite ratios 8, 19 -- In-Reply-To: {201109012130.p81LU32O011626-at-ns.microscopy.com} 8, 19 -- References: {201109012130.p81LU32O011626-at-ns.microscopy.com} 8, 19 -- Mime-Version: 1.0 8, 19 -- Content-Type: text/plain; charset="us-ascii"; format=flowed ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arturr.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arturr.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] emission current problem
Message: Hi all
Increasing heat make to increase Emission current in EPMA SX100. for instance we set Hv in 15 Kev, heat 198 and Emission current to 80 but when we clicking to preview other number are shown specially EMI current increasing to 426 and at the end we couldn't set up the calibration. why it is happening? also during set up calibration, there is errors like this: error from gun current.
also which problems may effect the gun current and emission current and which aleged to considering part?
I need more urgent help.please every one know them guide me.
Hydrofluoric acid is a very hazardous material. I am a chemist and I avoid using it wherever possible. You can not use it in a regular jet polishing system, such as a Struers Tenupol, as the acid will etch the windows of the light sensor. I have no direct experience with Ta. However, a universal electropolishing solution which I have used on literally dozens of metals, is 4% HClO4 in methanol at -40°C, using about 120-160mA (for double sided polishing). This includes stainless steels, superalloys, Al and Mg alloys, cast iron, copper alloys, rare earth metals, metallic glasses etc.
HClO4 (perchloric acid) has its own hazards, but I would use it over HF any day. I can't guarantee that it will work with Ta, but if it was me, I'd be trying it. The HF-containing solutions would be the last resort.
Regards,
Dave Mitchell
On 2/09/11 12:13 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both dam-at-fzu.cz as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: dam-at-fzu.cz Name: Karel Dám } } Title-Subject: [Filtered] Tantalum electropolishing } } Message: Dear listers, } } I would like to prepare TEM samples from almost pure tantalum by } electropolishing but I´m not sure about the conditions. I have found } someone used an electrolyte of sulphupic (5%) and hydrofluoric (2%) acid } in methanol. Does anyone have experience with this material? What } electrolyte composition, voltage, sample thickness and temperature do } you use? } } Any help will be very appreciated. } Thanks } regards } } Karel } } Karel Dám, MSc } Department of Physics AS CR, v. v. i. } Na Slovance 2 } CZ-182 21 Prague 8 } Czech Republic } Email: dam-at-fzu.cz } } Login Host: 147.231.26.31 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Sep 1 09:02:24 } 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p81E2Oes025496 } 11, 25 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:24 -0500 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id AAEB24052F2 } 11, 25 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:24 -0500 } (CDT) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } 10024) } 11, 25 -- with ESMTP id 2TtPe9ksWBS0 for {microscopy-at-microscopy.com} ; } 11, 25 -- Thu, 1 Sep 2011 09:02:24 -0500 (CDT) } 11, 25 -- Received: from NestorPwrBk.local (unknown [81.92.19.138]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id 63F5B4052E2 } 11, 25 -- for {microscopy-at-microscopy.com} ; Thu, 1 Sep 2011 09:02:23 -0500 } (CDT) } 11, 25 -- Message-ID: {4E5F906C.8050003-at-microscopy.com} } 11, 25 -- Date: Thu, 01 Sep 2011 16:02:20 +0200 } 11, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 11, 25 -- Reply-To: dam-at-fzu.cz } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: [Filtered] viaWWW: Tantalum electropolishing } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun Sep 4 09:27:04 2011 14, 25 -- Received: from znl.com ([206.69.208.20]) 14, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p84ER4nQ007520 14, 25 -- for {microscopy-at-microscopy.com} ; Sun, 4 Sep 2011 09:27:04 -0500 14, 25 -- Received: from localhost (localhost [127.0.0.1]) 14, 25 -- by znl.com (Postfix) with ESMTP id 6AE9C4077CA 14, 25 -- for {microscopy-at-microscopy.com} ; Sun, 4 Sep 2011 09:27:04 -0500 (CDT) 14, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 14, 25 -- Received: from znl.com ([127.0.0.1]) 14, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 14, 25 -- with ESMTP id hfp91D3z7GhF for {microscopy-at-microscopy.com} ; 14, 25 -- Sun, 4 Sep 2011 09:27:00 -0500 (CDT) 14, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 14, 25 -- by znl.com (Postfix) with ESMTPA id D0BA14077BC 14, 25 -- for {microscopy-at-microscopy.com} ; Sun, 4 Sep 2011 09:27:00 -0500 (CDT) 14, 25 -- Message-ID: {4E638AB4.6010701-at-microscopy.com} 14, 25 -- Date: Sun, 04 Sep 2011 09:27:00 -0500 14, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 14, 25 -- Reply-To: David Mitchell {drg.mitchell-at-sydney.edu.au} 14, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 14, 25 -- MIME-Version: 1.0 14, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 25 -- Subject: [Filtered] Re: [Microscopy] viaWWW: Tantalum electropolishing 14, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Sometime ago, we salvaged a JEOL T220A SEM. In the process, we set the column aside for demonstrations, and the removable parts in a box as used parts - should anyone ever ask.
The URL with the link to a list of those parts is: http://cmirt.wcupa.edu/CMIRT_JEOL_T220_Parts.html (Please disregard any mention of cost other than shipping.)
I will be 'discarding' the lot (except the column) in two weeks - that is, on 22 September, 2011.
Cheers,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl ========================================================================= Abraham Lincoln was perhaps the greatest orator the United States has known since the days of the Founders. Read what he said, then, think and learn. http://showcase.netins.net/web/creative/lincoln/speeches/lyceum.htm And, what's a lyceum? http://dig.lib.niu.edu/ISHS/ishs-1990spring/ishs-1990spring45.pdf ========================================================================= History is Cyclic. Only the ism's, nouns or adjectives change, AND......, When the objective classroom turns to agenda, history repeats! ========================================================================= "Pan-Germanism was an achievement of intellectuals and writers. The professors of history, law, economics, political science, geography, and philosophy were its most uncompromising advocates. They converted the students of the universities to their ideas. Very soon the graduates made more converts. As teachers in the field of higher education (in the famous German Gymnasium and educational institutions of the same rank), as lawyers, judges, civil servants, and diplomats they had ample opportunity to serve their cause."
""Soap capital" desires more cleanliness, "building capital" a greater demand for homes, "publishing capital" more and better education, and "armaments capital" bigger armaments. The shortrun interests of every branch of business encourage such attitudes. In the long run, however, increased demand results in an inflow of more capital into the booming branch, and the competition of the new enterprises cuts down the profits." Ludwig von Mises (http://www.mises.org/etexts/mises/og/chap6.asp) ========================================================================= Thus, the wise state, looking for the best result, will support research in those endeavors that promise improvements in the 'general welfare'. The 'wise state' will know that one cannot order up the solution to any problem by treating the process of innovation as little more than magic. The 'unwise state' will create chaos. (FCM, 2011) =========================================================================
==============================Original Headers============================== 12, 34 -- From FMonson-at-wcupa.edu Tue Sep 6 10:35:30 2011 12, 34 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 12, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p86FZUHl002078 12, 34 -- for {microscopy-at-microscopy.com} ; Tue, 6 Sep 2011 10:35:30 -0500 12, 34 -- X-ASG-Debug-ID: 1315323329-0378c35f9d250930001-4CH8be 12, 34 -- Received: from WCU-XCH-01.PASSHE.LCL (wcu-xch-01.wcupa.edu [144.26.0.85]) by MX02.WCUPA.EDU with ESMTP id Xp5xQkJeOLKV6pCO (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO) for {microscopy-at-microscopy.com} ; Tue, 06 Sep 2011 11:35:29 -0400 (EDT) 12, 34 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 12, 34 -- X-Barracuda-Apparent-Source-IP: 144.26.0.85 12, 34 -- X-ASG-Whitelist: Client 12, 34 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 12, 34 -- WCU-XCH-01.PASSHE.LCL ([fe80::d594:aa4c:f7e5:2e16%16]) with mapi id 12, 34 -- 14.01.0270.001; Tue, 6 Sep 2011 11:35:29 -0400 12, 34 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 12, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 34 -- Subject: JEOL T220 Parts 12, 34 -- Thread-Topic: JEOL T220 Parts 12, 34 -- X-ASG-Orig-Subj: JEOL T220 Parts 12, 34 -- Thread-Index: Acxsqpr8p6XniWKAS/qZm+nmMMlzPQ== 12, 34 -- Date: Tue, 6 Sep 2011 15:35:29 +0000 12, 34 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C0ACBC7-at-WCU-XCH-03.PASSHE.LCL} 12, 34 -- Accept-Language: en-US 12, 34 -- Content-Language: en-US 12, 34 -- X-MS-Has-Attach: 12, 34 -- X-MS-TNEF-Correlator: 12, 34 -- x-originating-ip: [10.28.57.79] 12, 34 -- Content-Type: text/plain; charset="us-ascii" 12, 34 -- MIME-Version: 1.0 12, 34 -- X-Barracuda-Connect: wcu-xch-01.wcupa.edu[144.26.0.85] 12, 34 -- X-Barracuda-Start-Time: 1315323329 12, 34 -- X-Barracuda-Encrypted: AES128-SHA 12, 34 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 12, 34 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 12, 34 -- Content-Transfer-Encoding: 8bit 12, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p86FZUHl002078 ==============================End of - Headers==============================
The following position has just been posted for the National Museum of Natural History.
Please do not reply to this message- rather contact Tim Rose (roset-at-si.edu) for further information.
{} {} {} } {} {} Begin Message {} {} {} {} {} {}
Position Opening: Museum Specialist (Geology)
The Department of Mineral Sciences at the National Museum of Natural History in Washington, D.C. has a position opening in our analytical laboratories. We are seeking a person to assist a small group of full time researchers engaged in the study of meteorites, mineralogy, volcanology, igneous and metamorphic petrology, experimental petrology and biomineralogy. The duties of this position include sample preparation, fabrication of custom-designed parts and sample holders and assistance in the Analytical Scanning Electron Microscope and Electron Microprobe Laboratories. Preparation of thin sections and polished mounts of a wide variety of materials including Antarctic meteorites, rocks and experimental products is an essential part of this position. The position also includes basic machining and fabrication of parts from a variety of materials for use in the analytical and experimental laboratories. In addition, this person will serve in a backup role in our electron beam laboratories assisting users and troubleshooting hardware and software issues. We are looking for self-motivated applicants with a background in geologic sciences and the ability to work well in an exciting workplace with many challenges. All applicants must apply online at the USAJOBS website. No other applications can be accepted. To apply please go to (open to the public):
Visit our website for more information about us: http://mineralsciences.si.edu/ or contact Tim Rose (roset-at-si.edu), Analytical Laboratories Manager
{} {} {} } {} {} End Message {} {} {} {} {} {}
Scott Whittaker Chief NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
==============================Original Headers============================== 15, 26 -- From WHITTAKS-at-si.edu Tue Sep 6 14:59:57 2011 15, 26 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178]) 15, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p86JxvGt022415 15, 26 -- for {microscopy-at-microscopy.com} ; Tue, 6 Sep 2011 14:59:57 -0500 15, 26 -- Received: from SI-MSEHUB-N01.US.SINET.SI.EDU (si-msehubi-01.us.sinet.si.edu [160.111.49.142]) 15, 26 -- by si-mailout04.si.edu (Postfix) with ESMTP id B195FFC26 15, 26 -- for {microscopy-at-microscopy.com} ; Tue, 6 Sep 2011 15:59:56 -0400 (EDT) 15, 26 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::f1ea:a780:6df5:c7bd]) by 15, 26 -- SI-MSEHUB-N01.US.SINET.SI.EDU ([fe80::34ec:9394:533a:63ad%11]) with mapi; 15, 26 -- Tue, 6 Sep 2011 15:59:56 -0400 15, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 15, 26 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 15, 26 -- Date: Tue, 6 Sep 2011 15:59:55 -0400 15, 26 -- Subject: Position Announcement- Analytical EM - Was. DC 15, 26 -- Thread-Topic: Position Announcement- Analytical EM - Was. DC 15, 26 -- Thread-Index: Acxsz4tSwK86QNjyQMW/wttZaKU22Q== 15, 26 -- Message-ID: {038C04650A93A14381E944A473695F61948FE09D55-at-SI-MSEV03.US.SINET.SI.EDU} 15, 26 -- Accept-Language: en-US 15, 26 -- Content-Language: en-US 15, 26 -- X-MS-Has-Attach: 15, 26 -- X-MS-TNEF-Correlator: 15, 26 -- acceptlanguage: en-US 15, 26 -- Content-Type: text/plain; charset="iso-8859-1" 15, 26 -- MIME-Version: 1.0 15, 26 -- Content-Transfer-Encoding: 8bit 15, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p86JxvGt022415 ==============================End of - Headers==============================
Araz, I'm not familiar with your particular system, but since I haven't seen any replies, I'll give it a shot.
The first thing I would do is check to see if any tungsten whiskers have grown that are shorting the wehnelt to the filament (cathode), assuming you are running a tungsten filament. Cleaning will take care of that problem, if that is the problem.
If that is not the problem, then there is probably some kind of problem in the bias resistance circuit. It could be a shorted resistor or capacitor in the HV tank or it could be a problem with any automated bias control circuit that might exist. The problem could be located in the HV area or it could be low voltage control circuitry located elsewhere. There is also the possibility of a software glitch. You probably need to contact Cameca for more specific information.
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Sunday, September 04, 2011 10:31 AM To: kenconverse-at-qualityimages.biz
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both arturr.irani-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: arturr.irani-at-yahoo.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] emission current problem
Message: Hi all
Increasing heat make to increase Emission current in EPMA SX100. for instance we set Hv in 15 Kev, heat 198 and Emission current to 80 but when we clicking to preview other number are shown specially EMI current increasing to 426 and at the end we couldn't set up the calibration. why it is happening? also during set up calibration, there is errors like this: error from gun current.
also which problems may effect the gun current and emission current and which aleged to considering part?
I need more urgent help.please every one know them guide me.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jacqueline.ayotte-at-ticona.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I am looking to purchase a CT Scanner in order to view voids in plastic parts and possibly glass fiber orientation in parts without destroying the parts.
If anyone has a CT Scanner and is using it for similar purposes, I would be interested in knowing if you are happy with the equipment and software. If so, I'd like to know who the manufacturer of the equipment is and what make and model you have (if you can share this information).
My company is located in northern KY, USA - I hope to meet with a local sales representative. Thank you for your help.
Best Regards Jackie Ayotte Analytical Microscopist / Spectroscopist
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both l.kastrup-at-abberior.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: l.kastrup-at-abberior.com Name: Lars Kastrup
Dear listers Does anyone have experience of embedding and sectioning Mg particles. The particles have a very thin (a few nm) outer layer of Ti and the researcher needs to examine in TEM. He has embedded some particles and I have attempted to cut sections but the sperical particles roll up as the sections cut leaving holes in the sections. I think the particles are too densely packed in the block so there is little resin support around the spheres. Any tips on the best resin and cutting parameters to use would be very much appreciated before I get too frustrated!! Best wishes all Nicola WestonThis message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.
This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 1, 34 -- From Nicola.Weston-at-nottingham.ac.uk Wed Sep 7 08:39:33 2011 1, 34 -- Received: from thb-mta-19.emailfiltering.com (thb-mta-19-tx.emailfiltering.com [194.116.199.150]) 1, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p87DdW3A006289 1, 34 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2011 08:39:33 -0500 1, 34 -- Received: from smtp2.nottingham.ac.uk ([128.243.44.5]) 1, 34 -- by thb-mta-19.emailfiltering.com with emfmta (version 4.8.3.54) vanilla id 2103837970 1, 34 -- for microscopy-at-microscopy.com;dd1dae51e4c4dbe5; Wed, 07 Sep 2011 14:39:32 +0100 1, 34 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) 1, 34 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) 1, 34 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 1, 34 -- id 1R1ICQ-00068o-1p 1, 34 -- for microscopy-at-microscopy.com; Wed, 07 Sep 2011 14:29:54 +0100 1, 34 -- Received: from UIWEXHUB02.ad.nottingham.ac.uk ([128.243.15.132]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 1, 34 -- Wed, 7 Sep 2011 14:29:01 +0100 1, 34 -- Received: from EXCHANGE1.ad.nottingham.ac.uk ([fe80::60c2:2879:5a5a:29a6]) by 1, 34 -- UIWEXHUB02.ad.nottingham.ac.uk ([2002:80f3:f84::80f3:f84]) with mapi; Wed, 7 1, 34 -- Sep 2011 14:29:01 +0100 1, 34 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 1, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 1, 34 -- Date: Wed, 7 Sep 2011 14:29:01 +0100 1, 34 -- Subject: Ultramicrotomy of Mg particles 1, 34 -- Thread-Topic: Ultramicrotomy of Mg particles 1, 34 -- Thread-Index: AcxtYhrEQ6ayJTboTim6Y7xZfOt6FA== 1, 34 -- Message-ID: {443CA551C991D84AB0FF11A607D4D46C0BE3CFF355-at-EXCHANGE1.ad.nottingham.ac.uk} 1, 34 -- Accept-Language: en-US, en-GB 1, 34 -- Content-Language: en-US 1, 34 -- X-MS-Has-Attach: 1, 34 -- X-MS-TNEF-Correlator: 1, 34 -- acceptlanguage: en-US, en-GB 1, 34 -- Content-Type: text/plain; charset="us-ascii" 1, 34 -- MIME-Version: 1.0 1, 34 -- X-OriginalArrivalTime: 07 Sep 2011 13:29:02.0117 (UTC) FILETIME=[1B6BBD50:01CC6D62] 1, 34 -- Content-Transfer-Encoding: 8bit 1, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p87DdW3A006289 ==============================End of - Headers==============================
I tried microtoming a variety of aluminum alloy particles years ago and succeeded mostly in making aluminum nano-potato chips. A better bet would be to try a FIB on the embedded composite, or, depending on the size of the particles, it may even be possibly to lift out sections of single particles.
John Mardinly ASU
On Sep 7, 2011, at 6:48 AM, "Nicola.Weston-at-nottingham.ac.uk" {Nicola.Weston-at-nottingham.ac.uk} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers } Does anyone have experience of embedding and sectioning Mg particles. The particles have a very thin (a few nm) outer layer of Ti and the researcher needs to examine in TEM. He has embedded some particles and I have attempted to cut sections but the sperical particles roll up as the sections cut leaving holes in the sections. I think the particles are too densely packed in the block so there is little resin support around the spheres. Any tips on the best resin and cutting parameters to use would be very much appreciated before I get too frustrated!! } Best wishes all } Nicola WestonThis message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. } } This message has been checked for viruses but the contents of an attachment } may still contain software viruses which could damage your computer system: } you are advised to perform your own checks. Email communications with the } University of Nottingham may be monitored as permitted by UK legislation. } } ==============================Original Headers============================== } 1, 34 -- From Nicola.Weston-at-nottingham.ac.uk Wed Sep 7 08:39:33 2011 } 1, 34 -- Received: from thb-mta-19.emailfiltering.com (thb-mta-19-tx.emailfiltering.com [194.116.199.150]) } 1, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p87DdW3A006289 } 1, 34 -- for {microscopy-at-microscopy.com} ; Wed, 7 Sep 2011 08:39:33 -0500 } 1, 34 -- Received: from smtp2.nottingham.ac.uk ([128.243.44.5]) } 1, 34 -- by thb-mta-19.emailfiltering.com with emfmta (version 4.8.3.54) vanilla id 2103837970 } 1, 34 -- for microscopy-at-microscopy.com;dd1dae51e4c4dbe5; Wed, 07 Sep 2011 14:39:32 +0100 } 1, 34 -- Received: from suismtp1.ad.nottingham.ac.uk ([128.243.42.10]) } 1, 34 -- by smtp2.nottingham.ac.uk with esmtp (Exim 4.60) } 1, 34 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) } 1, 34 -- id 1R1ICQ-00068o-1p } 1, 34 -- for microscopy-at-microscopy.com; Wed, 07 Sep 2011 14:29:54 +0100 } 1, 34 -- Received: from UIWEXHUB02.ad.nottingham.ac.uk ([128.243.15.132]) by SUISMTP1.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); } 1, 34 -- Wed, 7 Sep 2011 14:29:01 +0100 } 1, 34 -- Received: from EXCHANGE1.ad.nottingham.ac.uk ([fe80::60c2:2879:5a5a:29a6]) by } 1, 34 -- UIWEXHUB02.ad.nottingham.ac.uk ([2002:80f3:f84::80f3:f84]) with mapi; Wed, 7 } 1, 34 -- Sep 2011 14:29:01 +0100 } 1, 34 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} } 1, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 1, 34 -- Date: Wed, 7 Sep 2011 14:29:01 +0100 } 1, 34 -- Subject: Ultramicrotomy of Mg particles } 1, 34 -- Thread-Topic: Ultramicrotomy of Mg particles } 1, 34 -- Thread-Index: AcxtYhrEQ6ayJTboTim6Y7xZfOt6FA== } 1, 34 -- Message-ID: {443CA551C991D84AB0FF11A607D4D46C0BE3CFF355-at-EXCHANGE1.ad.nottingham.ac.uk} } 1, 34 -- Accept-Language: en-US, en-GB } 1, 34 -- Content-Language: en-US } 1, 34 -- X-MS-Has-Attach: } 1, 34 -- X-MS-TNEF-Correlator: } 1, 34 -- acceptlanguage: en-US, en-GB } 1, 34 -- Content-Type: text/plain; charset="us-ascii" } 1, 34 -- MIME-Version: 1.0 } 1, 34 -- X-OriginalArrivalTime: 07 Sep 2011 13:29:02.0117 (UTC) FILETIME=[1B6BBD50:01CC6D62] } 1, 34 -- Content-Transfer-Encoding: 8bit } 1, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p87DdW3A006289 } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 39 -- From John.Mardinly-at-asu.edu Wed Sep 7 16:24:31 2011 6, 39 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p87LOVrD029868 6, 39 -- for {Microscopy-at-Microscopy.com} ; Wed, 7 Sep 2011 16:24:31 -0500 6, 39 -- X-ASG-Debug-ID: 1315430667-03608c3a1427b720001-FOsErg 6, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet7.asu.edu with ESMTP id SDme1ssXuhf7XMC8 (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Wed, 07 Sep 2011 14:24:27 -0700 (MST) 6, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 6, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 6, 39 -- X-ASG-Whitelist: Client 6, 39 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.206) by 6, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 6, 39 -- 8.2.255.0; Wed, 7 Sep 2011 14:24:20 -0700 6, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by 6, 39 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Wed, 7 Sep 6, 39 -- 2011 14:24:21 -0700 6, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 6, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Wed, 7 Sep 2011 6, 39 -- 21:24:20 +0000 6, 39 -- Subject: Re: [Microscopy] Ultramicrotomy of Mg particles 6, 39 -- References: {201109071347.p87DlZDq021602-at-ns.microscopy.com} 6, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] Ultramicrotomy of Mg particles 6, 39 -- thread-topic: [Microscopy] Ultramicrotomy of Mg particles 6, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} 6, 39 -- thread-index: AcxtpIGeL0KetU38RaOCFo727Gil0w== 6, 39 -- Content-Type: text/plain; charset="us-ascii" 6, 39 -- In-Reply-To: {201109071347.p87DlZDq021602-at-ns.microscopy.com} 6, 39 -- Message-ID: {8DF8C462-7306-41FA-B7D3-194E64518CA0-at-exchange.asu.edu} 6, 39 -- Date: Wed, 7 Sep 2011 14:28:32 -0700 6, 39 -- To: {Nicola.Weston-at-nottingham.ac.uk} 6, 39 -- CC: "MSA" {Microscopy-at-Microscopy.com} 6, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) 6, 39 -- X-OriginalArrivalTime: 07 Sep 2011 21:24:21.0047 (UTC) FILETIME=[82071C70:01CC6DA4] 6, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 6, 39 -- X-Barracuda-Start-Time: 1315430667 6, 39 -- X-Barracuda-Encrypted: RC4-MD5 6, 39 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 6, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu 6, 39 -- Content-Transfer-Encoding: 8bit 6, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p87LOVrD029868 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibera-at-stevens.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibera-at-stevens.edu Name: Matthew Libera
Message: On Tuesday September 27, 2011 there will be a one-day workshop at Stevens Institute of Technology in Hoboken, New Jersey addressing the current state of the art at the confluence of scanning electron microscopy, focused ion-beam processing, and cryogenic specimen manipulation methods. More details and registration information can be found at the Workshop website: http://www.stevens.edu/lmsi/workshop/
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bradbury.arcana-at-btinternet.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bradbury.arcana-at-btinternet.com
Name: david bradabuey
Organization: retired
Title-Subject: [Filtered] Spectroscopy/microscopy
Message: Hi Folks.
Has anyone an old spectrometer (U/V Vis) they wish to donate to an old timer, I am presently researching micrometeroites and bactiological magnetite, I am willing to collect or pay postage if you can help. UK only please.
Thanks all for your ideas. I should of course have added the particle size. DOH! Spheres are ranging from 30 to over 100 microns so my usual powder prep technique will not work. I think I'll send him over to another department which has a FIB-SEM!
I may persevere with some sectioning though as it's all good practice.This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham.
This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.
==============================Original Headers============================== 2, 34 -- From Nicola.Weston-at-nottingham.ac.uk Thu Sep 8 07:10:23 2011 2, 34 -- Received: from ixe-mta-19.emailfiltering.com (ixe-mta-19-tx.emailfiltering.com [194.116.198.150]) 2, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p88CAMW8016109 2, 34 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 07:10:22 -0500 2, 34 -- Received: from smtp1.nottingham.ac.uk ([128.243.44.4]) 2, 34 -- by ixe-mta-19.emailfiltering.com with emfmta (version 4.8.3.54) vanilla id 1964406548 2, 34 -- for microscopy-at-microscopy.com;8d07afda0a1d04f1; Thu, 08 Sep 2011 13:10:21 +0100 2, 34 -- Received: from suismtp2.ad.nottingham.ac.uk ([128.243.42.11]) 2, 34 -- by smtp1.nottingham.ac.uk with esmtp (Exim 4.60) 2, 34 -- (envelope-from {Nicola.Weston-at-nottingham.ac.uk} ) 2, 34 -- id 1R1cip-0005H4-TX 2, 34 -- for microscopy-at-microscopy.com; Thu, 08 Sep 2011 12:24:43 +0100 2, 34 -- Received: from UIWEXHUB01.ad.nottingham.ac.uk ([128.243.15.133]) by SUISMTP2.ad.nottingham.ac.uk with Microsoft SMTPSVC(6.0.3790.4675); 2, 34 -- Thu, 8 Sep 2011 12:25:19 +0100 2, 34 -- Received: from EXCHANGE1.ad.nottingham.ac.uk ([fe80::60c2:2879:5a5a:29a6]) by 2, 34 -- UIWEXHUB01.ad.nottingham.ac.uk ([2002:80f3:f85::80f3:f85]) with mapi; Thu, 8 2, 34 -- Sep 2011 12:25:19 +0100 2, 34 -- From: Nicola Weston {Nicola.Weston-at-nottingham.ac.uk} 2, 34 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 34 -- Date: Thu, 8 Sep 2011 12:25:19 +0100 2, 34 -- Subject: Ultramicrotomy of Mg particles- Thanks 2, 34 -- Thread-Topic: Ultramicrotomy of Mg particles- Thanks 2, 34 -- Thread-Index: AcxuGf10f/TJVKoBQAGMG8AseOFrNA== 2, 34 -- Message-ID: {443CA551C991D84AB0FF11A607D4D46C0BE3E1A408-at-EXCHANGE1.ad.nottingham.ac.uk} 2, 34 -- Accept-Language: en-US, en-GB 2, 34 -- Content-Language: en-US 2, 34 -- X-MS-Has-Attach: 2, 34 -- X-MS-TNEF-Correlator: 2, 34 -- acceptlanguage: en-US, en-GB 2, 34 -- Content-Type: text/plain; charset="us-ascii" 2, 34 -- MIME-Version: 1.0 2, 34 -- X-OriginalArrivalTime: 08 Sep 2011 11:25:19.0982 (UTC) FILETIME=[FDE590E0:01CC6E19] 2, 34 -- Content-Transfer-Encoding: 8bit 2, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p88CAMW8016109 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scopes.master-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scopes.master-at-gmail.com
Name: Hamid R. Shakeri
Organization: Aviceena Metallurgical Research Services
Title-Subject: [Filtered] Do you know the person who operated this system in Maryland?
Message: Hello to all, I am looking for the person who used to operate a Cambridge Stereoscan 180 at the following address: Advanced Biotech 9108 Guilford Road Columbia, MD 21045
We have acquired this system trying to make it work again and if could get in touch with the previous operator, it will be a great help for us. If you know this person please pass my email address: scopes.master-at-gmail.com to her/him.
-- I recently had someone put a sample in our TEM that gummed up the holder and contaminated the objective aperture. Another person lost their support film and it dropped into the lenses necessitating another service call. Two other people have asked me to allow them to also put in questionable samples. We don't do any thin sectioning here so it is all particulate samples. When I train individuals I ask what their samples will be but many times, after they are trained, these same people take on samples from colleagues and it is those samples that I worry about.
What do some of the rest of you do in situations like this? I am running a multi-user facility and people run their own samples after they are trained. Do any of you have a banned list of substances that you don't allow in your instruments?
Norm Olson
______________________________________________________________ Norm Olson Cryoelectron Microscopy Facilities Manager 1510 Bonner Hall Department of Chemistry & Biochemistry, MC-0378 University of California San Diego La Jolla, CA 92093-0378 nholson-at-ucsd.edu http://cryoem.ucsd.edu Cell: (858)220-2183 (858)822-6718 - Office; (858)534-5846 - Fax ______________________________________________________________
You cannot monitor each person and their samples. You just have to try to teach them what is an appropriate and then trust them to act accordingly.
It sounds like the one person used a support grid with too much sample on it, thus getting residue on the holder. Likely this sample "boiled in the beam and that was what contaminated your aperture. I tell them that, first of all, you should not be able to see the sample on the grid. If you do than it is probably too thick. Secondly, the grid must be dry before inserting, and third, if any sign of sample instability is seen than they must immediately remove it from the microscope. This usually means there is residual material that is not stable when exposed to the heat and energy of the electron beam.
The other person had a poorly prepared sample as well. Most likely it was not firmly in the holder and thus fell off the holder. I would think any support film that was not well adhered to the grid would normally come off during sample preparation. This should certainly not happen with any sample and is not a problem with sectioned material. The sections must adhere well to the support grid or they would not withstand subsequent staining. It is pretty easy to hold a grid up to light and see if the surface is reflective due to the presence of a film. If some squares are dark and others light than you know the film is not covering the entire grid.
The instruments are there to be used so I would not like to indiscriminately ban samples. However, users should be encouraged to talk to you before they put questionable samples in the scope so that you can both brain-storm about possible problems and ways to solve them. Sometimes this can just be sandwiching the sample by putting an extra layer on top of the sample...either carbon or formvar film will work.
If there is a way to mess up a scope a student will find it. That is just one of the many reasons for training users thoroughly on not just the microscope but also sample preparation and then all you can do is hope they absorb the message.
Just my two cents from years of experience.
Debby -- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy/
} From: Norman Olson {nholson-at-ucsd.edu} } Reply-To: Norman Olson {nholson-at-ucsd.edu} } Date: Thu, 8 Sep 2011 14:37:06 -0400 } To: Debby Sherman {dsherman-at-purdue.edu} } Subject: [Microscopy] banned substances } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } -- } I recently had someone put a sample in our TEM that gummed up the } holder and contaminated the objective aperture. Another person lost } their support film and it dropped into the lenses necessitating } another service call. Two other people have asked me to allow them } to also put in questionable samples. We don't do any thin sectioning } here so it is all particulate samples. When I train individuals I } ask what their samples will be but many times, after they are } trained, these same people take on samples from colleagues and it is } those samples that I worry about. } } What do some of the rest of you do in situations like this? I am } running a multi-user facility and people run their own samples after } they are trained. Do any of you have a banned list of substances } that you don't allow in your instruments? } } Norm Olson } } } ______________________________________________________________ } Norm Olson } Cryoelectron Microscopy Facilities Manager } 1510 Bonner Hall } Department of Chemistry & Biochemistry, MC-0378 } University of California San Diego } La Jolla, CA 92093-0378 } nholson-at-ucsd.edu } http://cryoem.ucsd.edu } Cell: (858)220-2183 } (858)822-6718 - Office; (858)534-5846 - Fax } ______________________________________________________________ } } ==============================Original Headers============================== } 6, 38 -- From nholson-at-ucsd.edu Thu Sep 8 13:34:48 2011 } 6, 38 -- Received: from iport-c2-out.ucsd.edu (iport-c2-out.ucsd.edu } [132.239.0.119]) } 6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p88IYm9R025066 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 13:34:48 -0500 } 6, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 6, 38 -- d=ucsd.edu; i=nholson-at-ucsd.edu; q=dns/txt; } 6, 38 -- s=041709-iport; t=1315506889; x=1347042889; } 6, 38 -- h=mime-version:message-id:date:to:from:subject; } 6, 38 -- z=Mime-Version:=201.0|Message-Id:=20 {p0624083bca8eb908d4d2 } 6, 38 -- -at-[132.239.70.186]} |Date:=20Thu,=208=20Sep=202011=2011:34: } 6, 38 -- 45=20-0700|To:=20Microscopy-at-microscopy.com|From:=20Norm } 6, 38 -- =20Olson=20 {nholson-at-ucsd.edu} |Subject:=20banned=20substan } 6, 38 -- ces; } 6, 38 -- bh=9Gr7dePBQlvXrAMGbmAc4uYzUlVIdasRvuEqv3LnO+s=; } 6, 38 -- b=BOUex3CxAHEUTHpwgXK7lH2NGJOiJGwegJt8cSkQ1l8ORDwWvo2F6QiR } 6, 38 -- lcJbNNjbetkal9CdcW1Qu1xkVRwzkuEmrNGBjHy3/cNUeCNqh4jxaLFWZ } 6, 38 -- dEoHL3cPNGV2p00/plMKeLGPRajV5p30hERprooAvrjAJs37chckMzQVY } 6, 38 -- A=; } 6, 38 -- X-IronPort-Anti-Spam-Filtered: true } 6, 38 -- X-IronPort-Anti-Spam-Result: } AhIKAJEJaU6E7/kU/2dsb2JhbAA/A5kwAQGOUHiCB01DJjsfoA6WToh/AoNfgwwEh2yQSg+Ea4c5 } 6, 38 -- X-IronPort-AV: E=Sophos;i="4.68,351,1312182000"; } 6, 38 -- d="scan'208";a="602953960" } 6, 38 -- X-Spam-Status: No } 6, 38 -- X-Spam-Level: } 6, 38 -- Received: from smtp-tpcs.ucsd.edu ([132.239.249.20]) } 6, 38 -- by iport-c2-out.ucsd.edu with ESMTP; 08 Sep 2011 11:34:47 -0700 } 6, 38 -- Received: from [132.239.70.186] (patrick-70-186.ucsd.edu } [132.239.70.186]) } 6, 38 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } 6, 38 -- (No client certificate requested) } 6, 38 -- by smtp-tpcs.ucsd.edu (Postfix) with ESMTPSA id 1C59680EE4 } 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 11:34:46 -0700 } (PDT) } 6, 38 -- Mime-Version: 1.0 } 6, 38 -- Message-Id: {p0624083bca8eb908d4d2-at-[132.239.70.186]} } 6, 38 -- Date: Thu, 8 Sep 2011 11:34:45 -0700 } 6, 38 -- To: Microscopy-at-microscopy.com } 6, 38 -- From: Norm Olson {nholson-at-ucsd.edu} } 6, 38 -- Subject: banned substances } 6, 38 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } ==============================End of - Headers==============================
We had one group of dental materials students at Michigan that were looking at human teeth. They did not attach the teeth properly, and they fell off the holders. Of course, they never admitted that to anyone, and they had so many teeth, they just kept putting more samples in until they got the photos they wanted. The next time I opened the specimen chamber, I found it full of human teeth. It did not take long to figure out where they came from. Just hope one of those clowns is not drilling out YOUR teeth the next time you go to the dentist!
On Sep 8, 2011, at 2:59 PM, "dsherman-at-purdue.edu" {dsherman-at-purdue.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Norm, } } You cannot monitor each person and their samples. You just have to try to } teach them what is an appropriate and then trust them to act accordingly. } } It sounds like the one person used a support grid with too much sample on } it, thus getting residue on the holder. Likely this sample "boiled in the } beam and that was what contaminated your aperture. I tell them that, first } of all, you should not be able to see the sample on the grid. If you do } than it is probably too thick. Secondly, the grid must be dry before } inserting, and third, if any sign of sample instability is seen than they } must immediately remove it from the microscope. This usually means there is } residual material that is not stable when exposed to the heat and energy of } the electron beam. } } The other person had a poorly prepared sample as well. Most likely it was } not firmly in the holder and thus fell off the holder. I would think any } support film that was not well adhered to the grid would normally come off } during sample preparation. This should certainly not happen with any sample } and is not a problem with sectioned material. The sections must adhere well } to the support grid or they would not withstand subsequent staining. It is } pretty easy to hold a grid up to light and see if the surface is reflective } due to the presence of a film. If some squares are dark and others light } than you know the film is not covering the entire grid. } } The instruments are there to be used so I would not like to indiscriminately } ban samples. However, users should be encouraged to talk to you before they } put questionable samples in the scope so that you can both brain-storm about } possible problems and ways to solve them. Sometimes this can just be } sandwiching the sample by putting an extra layer on top of the } sample...either carbon or formvar film will work. } } If there is a way to mess up a scope a student will find it. That is just } one of the many reasons for training users thoroughly on not just the } microscope but also sample preparation and then all you can do is hope they } absorb the message. } } Just my two cents from years of experience. } } Debby } -- } Debby Sherman, Director Phone: 765-494-6666 } Life Science Microscopy Facility FAX: 765-494-5896 } Purdue University E-mail: dsherman-at-purdue.edu } S-052 Whistler Building } 170 S. University Street } West Lafayette, IN 47907 } http://www.ag.purdue.edu/facilities/microscopy/ } } } } From: Norman Olson {nholson-at-ucsd.edu} } } Reply-To: Norman Olson {nholson-at-ucsd.edu} } } Date: Thu, 8 Sep 2011 14:37:06 -0400 } } To: Debby Sherman {dsherman-at-purdue.edu} } } Subject: [Microscopy] banned substances } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } } } -- } } I recently had someone put a sample in our TEM that gummed up the } } holder and contaminated the objective aperture. Another person lost } } their support film and it dropped into the lenses necessitating } } another service call. Two other people have asked me to allow them } } to also put in questionable samples. We don't do any thin sectioning } } here so it is all particulate samples. When I train individuals I } } ask what their samples will be but many times, after they are } } trained, these same people take on samples from colleagues and it is } } those samples that I worry about. } } } } What do some of the rest of you do in situations like this? I am } } running a multi-user facility and people run their own samples after } } they are trained. Do any of you have a banned list of substances } } that you don't allow in your instruments? } } } } Norm Olson } } } } } } ______________________________________________________________ } } Norm Olson } } Cryoelectron Microscopy Facilities Manager } } 1510 Bonner Hall } } Department of Chemistry & Biochemistry, MC-0378 } } University of California San Diego } } La Jolla, CA 92093-0378 } } nholson-at-ucsd.edu } } http://cryoem.ucsd.edu } } Cell: (858)220-2183 } } (858)822-6718 - Office; (858)534-5846 - Fax } } ______________________________________________________________ } } } } ==============================Original Headers============================== } } 6, 38 -- From nholson-at-ucsd.edu Thu Sep 8 13:34:48 2011 } } 6, 38 -- Received: from iport-c2-out.ucsd.edu (iport-c2-out.ucsd.edu } } [132.239.0.119]) } } 6, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p88IYm9R025066 } } 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 13:34:48 -0500 } } 6, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } } 6, 38 -- d=ucsd.edu; i=nholson-at-ucsd.edu; q=dns/txt; } } 6, 38 -- s=041709-iport; t=1315506889; x=1347042889; } } 6, 38 -- h=mime-version:message-id:date:to:from:subject; } } 6, 38 -- z=Mime-Version:=201.0|Message-Id:=20 {p0624083bca8eb908d4d2 } } 6, 38 -- -at-[132.239.70.186]} |Date:=20Thu,=208=20Sep=202011=2011:34: } } 6, 38 -- 45=20-0700|To:=20Microscopy-at-microscopy.com|From:=20Norm } } 6, 38 -- =20Olson=20 {nholson-at-ucsd.edu} |Subject:=20banned=20substan } } 6, 38 -- ces; } } 6, 38 -- bh=9Gr7dePBQlvXrAMGbmAc4uYzUlVIdasRvuEqv3LnO+s=; } } 6, 38 -- b=BOUex3CxAHEUTHpwgXK7lH2NGJOiJGwegJt8cSkQ1l8ORDwWvo2F6QiR } } 6, 38 -- lcJbNNjbetkal9CdcW1Qu1xkVRwzkuEmrNGBjHy3/cNUeCNqh4jxaLFWZ } } 6, 38 -- dEoHL3cPNGV2p00/plMKeLGPRajV5p30hERprooAvrjAJs37chckMzQVY } } 6, 38 -- A=; } } 6, 38 -- X-IronPort-Anti-Spam-Filtered: true } } 6, 38 -- X-IronPort-Anti-Spam-Result: } } AhIKAJEJaU6E7/kU/2dsb2JhbAA/A5kwAQGOUHiCB01DJjsfoA6WToh/AoNfgwwEh2yQSg+Ea4c5 } } 6, 38 -- X-IronPort-AV: E=Sophos;i="4.68,351,1312182000"; } } 6, 38 -- d="scan'208";a="602953960" } } 6, 38 -- X-Spam-Status: No } } 6, 38 -- X-Spam-Level: } } 6, 38 -- Received: from smtp-tpcs.ucsd.edu ([132.239.249.20]) } } 6, 38 -- by iport-c2-out.ucsd.edu with ESMTP; 08 Sep 2011 11:34:47 -0700 } } 6, 38 -- Received: from [132.239.70.186] (patrick-70-186.ucsd.edu } } [132.239.70.186]) } } 6, 38 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } } 6, 38 -- (No client certificate requested) } } 6, 38 -- by smtp-tpcs.ucsd.edu (Postfix) with ESMTPSA id 1C59680EE4 } } 6, 38 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 11:34:46 -0700 } } (PDT) } } 6, 38 -- Mime-Version: 1.0 } } 6, 38 -- Message-Id: {p0624083bca8eb908d4d2-at-[132.239.70.186]} } } 6, 38 -- Date: Thu, 8 Sep 2011 11:34:45 -0700 } } 6, 38 -- To: Microscopy-at-microscopy.com } } 6, 38 -- From: Norm Olson {nholson-at-ucsd.edu} } } 6, 38 -- Subject: banned substances } } 6, 38 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 12, 31 -- From dsherman-at-purdue.edu Thu Sep 8 16:01:50 2011 } 12, 31 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) } 12, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p88L1oln011076 } 12, 31 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 16:01:50 -0500 } 12, 31 -- Received: from WPPEXHUB02F.purdue.lcl (wppexhub02f.itap.purdue.edu [172.21.6.91]) } 12, 31 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p88L1ohf011688 } 12, 31 -- for {microscopy-at-microscopy.com} ; Thu, 8 Sep 2011 17:01:50 -0400 } 12, 31 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.54]) by WPPEXHUB02F.purdue.lcl } 12, 31 -- ([::1]) with mapi; Thu, 8 Sep 2011 17:01:49 -0400 } 12, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} } 12, 31 -- To: Norman Olson {nholson-at-ucsd.edu} , } 12, 31 -- "message to: MSA list" } 12, 31 -- {microscopy-at-microscopy.com} } 12, 31 -- Date: Thu, 8 Sep 2011 17:01:47 -0400 } 12, 31 -- Subject: Re: [Microscopy] banned substances } 12, 31 -- Thread-Topic: [Microscopy] banned substances } 12, 31 -- Thread-Index: AcxuVk/82mFFehFxRyC8yXc8YQJIQQAFDViQ } 12, 31 -- Message-ID: {CA8EA57B.12E6C%dsherman-at-purdue.edu} } 12, 31 -- In-Reply-To: {201109081837.p88Ib6X9027754-at-ns.microscopy.com} } 12, 31 -- Accept-Language: en-US } 12, 31 -- Content-Language: en-US } 12, 31 -- X-MS-Has-Attach: } 12, 31 -- X-MS-TNEF-Correlator: } 12, 31 -- user-agent: Microsoft-Entourage/13.10.0.110428 } 12, 31 -- acceptlanguage: en-US } 12, 31 -- Content-Type: text/plain; charset="us-ascii" } 12, 31 -- MIME-Version: 1.0 } 12, 31 -- X-PMX-Version: 5.5.9.388399 } 12, 31 -- X-PerlMx-Virus-Scanned: Yes } 12, 31 -- Content-Transfer-Encoding: 8bit } 12, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p88L1oln011076 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 38 -- From John.Mardinly-at-asu.edu Thu Sep 8 18:20:59 2011 8, 38 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p88NKvfi028860 8, 38 -- for {Microscopy-at-Microscopy.com} ; Thu, 8 Sep 2011 18:20:58 -0500 8, 38 -- X-ASG-Debug-ID: 1315524047-03efbe2242d6bd40012-FOsErg 8, 38 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet6.asu.edu with ESMTP id 3E8m6wWSNWG5ss3e (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Thu, 08 Sep 2011 16:20:52 -0700 (MST) 8, 38 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 8, 38 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 8, 38 -- X-ASG-Whitelist: Client 8, 38 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.206) by 8, 38 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 8, 38 -- 8.2.255.0; Thu, 8 Sep 2011 16:20:45 -0700 8, 38 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by 8, 38 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 8 Sep 8, 38 -- 2011 16:20:45 -0700 8, 38 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 8, 38 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Thu, 8 Sep 2011 8, 38 -- 23:20:45 +0000 8, 38 -- Subject: Re: [Microscopy] Re: banned substances 8, 38 -- References: {201109082110.p88LA5eZ026301-at-ns.microscopy.com} 8, 38 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: banned substances 8, 38 -- From: John Mardinly {John.Mardinly-at-asu.edu} 8, 38 -- thread-topic: [Microscopy] Re: banned substances 8, 38 -- thread-index: Acxufe9AJDDU5PKhQO6aH0iTi+NRAQ== 8, 38 -- Content-Type: text/plain; charset="us-ascii" 8, 38 -- In-Reply-To: {201109082110.p88LA5eZ026301-at-ns.microscopy.com} 8, 38 -- Message-ID: {03139CCE-A658-4180-B301-00B216A1C4AF-at-exchange.asu.edu} 8, 38 -- Date: Thu, 8 Sep 2011 16:24:58 -0700 8, 38 -- To: {dsherman-at-purdue.edu} , MSA {Microscopy-at-Microscopy.com} 8, 38 -- MIME-Version: 1.0 (iPad Mail 8G4) 8, 38 -- X-OriginalArrivalTime: 08 Sep 2011 23:20:45.0460 (UTC) FILETIME=[EF79B540:01CC6E7D] 8, 38 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 8, 38 -- X-Barracuda-Start-Time: 1315524052 8, 38 -- X-Barracuda-Encrypted: RC4-MD5 8, 38 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 8, 38 -- X-Virus-Scanned: by bsmtpd at asu.edu 8, 38 -- Content-Transfer-Encoding: 8bit 8, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p88NKvfi028860 ==============================End of - Headers==============================
Do you think price of $60,000 for 10 years old VP SEM (tungsten, Windows NT, turbomolecular pump, 100 mm X travel) is right? What can cost 10 years old EDS system (LN, basic software, no fancy staff)?
Thank you,
Vladimir
==============================Original Headers============================== 5, 29 -- From DusevichV-at-umkc.edu Fri Sep 9 08:47:44 2011 5, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p89DliG4026714 5, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 08:47:44 -0500 5, 29 -- X-IronPort-Anti-Spam-Filtered: true 5, 29 -- X-IronPort-Anti-Spam-Result: Al4HAIUYak6Dl3nN/2dsb2JhbABBoVSGO3iBUwEFOk8CAT4QMhcBDQEBBBsYtlmIf4YOYASHbZ0D 5, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 5, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Sep 2011 08:47:42 -0500 5, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by 5, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Fri, 9 Sep 2011 5, 29 -- 08:47:42 -0500 5, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 29 -- Date: Fri, 9 Sep 2011 08:47:41 -0500 5, 29 -- Subject: Used SEM cost 5, 29 -- Thread-Topic: Used SEM cost 5, 29 -- Thread-Index: AcxHsc69Hu/XiVO2SSy9af3ZJPKzGQnRRGbA 5, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3B386A06E6A-at-UM-EMAIL06.um.umsystem.edu} 5, 29 -- References: {201107211413.p6LEDwfL026098-at-ns.microscopy.com} 5, 29 -- In-Reply-To: {201107211413.p6LEDwfL026098-at-ns.microscopy.com} 5, 29 -- Accept-Language: en-US 5, 29 -- Content-Language: en-US 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- acceptlanguage: en-US 5, 29 -- Content-Type: text/plain; charset="us-ascii" 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p89DliG4026714 ==============================End of - Headers==============================
Buying or selling? Either way ... first, it depends on what VP SEM, what extras does it have, what condition is it in, and has it been maintained under service contract for all its life - and does it have a complete service history? But. Likely $60k is too high or way too high. Purchase price has to allow for the costs of deinstalling, packing, removing, and shipping the SEM to its new home. This will cost several thousand dollars.
The EDS system likely won't go for much or less. Especially if the condition of the LN2 dewar isn't documented (does it need to be baked out and pumped?), nor the condition of the detector window. Usually the EDS is included in the sale price of the SEM. Plus, how does the VP SEM in question compare to a new table-top or "portable SEM", which are typically low vacuum units, and sell for ~$70k-$85k. (Can be more depending on brand and bells and whistles.) Meaning, why pay $60k for a 10 year old SEM with whatever age and service issues it has when one could spend $10-15k more and get a brand new SEM with a warranty? Is the old SEM still capable of higher resolution work? Does the buyer need the kV range of the used SEM, or are the limited ranges of the lower-cost new SEMs sufficient? Etc.
One way to get an idea of the going price is to look at successful sales on ebay and publicsurplus.com (where many universities and the like sell used EMs and other equipment). And trawl the web for companies that buy old lab equipment and get prices from them. Purchase prices, not selling prices.
Phil
} Hi Listers, } } Do you think price of $60,000 for 10 years old VP SEM (tungsten, } Windows NT, turbomolecular pump, 100 mm X travel) is right? } What can cost 10 years old EDS system (LN, basic software, no fancy staff)? } } Thank you, } } Vladimir
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 7, 26 -- From oshel1pe-at-cmich.edu Fri Sep 9 09:15:57 2011 7, 26 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p89EFuCR011160 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 09:15:57 -0500 7, 26 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 7, 26 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p89E01LD030590 7, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 10:00:15 -0400 7, 26 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 7, 26 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.289.1; Fri, 9 Sep 7, 26 -- 2011 10:15:46 -0400 7, 26 -- MIME-Version: 1.0 7, 26 -- Message-ID: {a06240801ca8fccbececb-at-[141.209.160.249]} 7, 26 -- In-Reply-To: {201109091353.p89DrGnP031887-at-ns.microscopy.com} 7, 26 -- References: {201109091353.p89DrGnP031887-at-ns.microscopy.com} 7, 26 -- Date: Fri, 9 Sep 2011 10:15:44 -0400 7, 26 -- To: {Microscopy-at-microscopy.com} 7, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 7, 26 -- Subject: Re: [Microscopy] Used SEM cost 7, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 7, 26 -- X-Originating-IP: [141.209.160.249] 7, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 7, 26 -- X-Spam-Score: 0.80 () [Hold at 6.00] L_USD,L_USDD,L_USDOC,_L_LLEXCH,Bayes(0.0001:-0.5) 7, 26 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 7, 26 -- X-CanItPRO-Stream: default 7, 26 -- X-Canit-Stats-ID: 03FuC0fkF - 67aa62d6ea4b - 20110909 7, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Well, that is very interesting. You might inquire about the availability of parts for this instrument. Here is my opinion:
We have a 14 yr old TEM (tungsten) with turbopump that has been out of service for 3+ years due to lack of parts. Some time ago, on this listserver, I asked about the life expectancy of a modern TEM and SEM and the responses averaged 12-15 years. Appalling!
For a usable EDS system of that age, I think that $20-30K would be reasonable. After all, you can buy a nice SDD system these days for $80K or so.
John Bozzola
On Fri, Sep 9, 2011 at 8:49 AM, {DusevichV-at-umkc.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } Do you think price of $60,000 for 10 years old VP SEM (tungsten, Windows NT, turbomolecular pump, 100 mm X travel) is right? } What can cost 10 years old EDS system (LN, basic software, no fancy staff)? } } Thank you, } } Vladimir } } } ==============================Original Headers============================== } 5, 29 -- From DusevichV-at-umkc.edu Fri Sep  9 08:47:44 2011 } 5, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 5, 29 --     by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p89DliG4026714 } 5, 29 --     for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 08:47:44 -0500 } 5, 29 -- X-IronPort-Anti-Spam-Filtered: true } 5, 29 -- X-IronPort-Anti-Spam-Result: Al4HAIUYak6Dl3nN/2dsb2JhbABBoVSGO3iBUwEFOk8CAT4QMhcBDQEBBBsYtlmIf4YOYASHbZ0D } 5, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) } 5, 29 --  by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Sep 2011 08:47:42 -0500 } 5, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by } 5, 29 --  UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Fri, 9 Sep 2011 } 5, 29 --  08:47:42 -0500 } 5, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 5, 29 -- Date: Fri, 9 Sep 2011 08:47:41 -0500 } 5, 29 -- Subject: Used SEM cost } 5, 29 -- Thread-Topic: Used SEM cost } 5, 29 -- Thread-Index: AcxHsc69Hu/XiVO2SSy9af3ZJPKzGQnRRGbA } 5, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3B386A06E6A-at-UM-EMAIL06.um.umsystem.edu} } 5, 29 -- References: {201107211413.p6LEDwfL026098-at-ns.microscopy.com} } 5, 29 -- In-Reply-To: {201107211413.p6LEDwfL026098-at-ns.microscopy.com} } 5, 29 -- Accept-Language: en-US } 5, 29 -- Content-Language: en-US } 5, 29 -- X-MS-Has-Attach: } 5, 29 -- X-MS-TNEF-Correlator: } 5, 29 -- acceptlanguage: en-US } 5, 29 -- Content-Type: text/plain; charset="us-ascii" } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- Content-Transfer-Encoding: 8bit } 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p89DliG4026714 } ==============================End of - Headers============================== }
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 9, 20 -- From bozzola-at-siu.edu Fri Sep 9 10:54:20 2011 9, 20 -- Received: from mail-ew0-f45.google.com (mail-ew0-f45.google.com [209.85.215.45]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p89FsJb4029803 9, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 10:54:19 -0500 9, 20 -- Received: by ewy24 with SMTP id 24so962879ewy.32 9, 20 -- for {Microscopy-at-microscopy.com} ; Fri, 09 Sep 2011 08:54:18 -0700 (PDT) 9, 20 -- MIME-Version: 1.0 9, 20 -- Received: by 10.52.37.82 with SMTP id w18mr342102vdj.261.1315583658111; Fri, 9, 20 -- 09 Sep 2011 08:54:18 -0700 (PDT) 9, 20 -- Received: by 10.52.112.134 with HTTP; Fri, 9 Sep 2011 08:54:18 -0700 (PDT) 9, 20 -- In-Reply-To: {201109091349.p89DnB3E027896-at-ns.microscopy.com} 9, 20 -- References: {201109091349.p89DnB3E027896-at-ns.microscopy.com} 9, 20 -- Date: Fri, 9 Sep 2011 10:54:18 -0500 9, 20 -- Message-ID: {CAKeCJ9ycEaQ7B=jXgouxrFD4rrPXZq8nDbP9SS26fjd+jvcehA-at-mail.gmail.com} 9, 20 -- Subject: Re: [Microscopy] Used SEM cost 9, 20 -- From: John Bozzola {bozzola-at-siu.edu} 9, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 9, 20 -- Content-Type: text/plain; charset=UTF-8 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p89FsJb4029803 ==============================End of - Headers==============================
What's truly appalling is that I'm still running 2 old 201s (~36yrs old) and a CM10 (~25yrs). But then, I have a 3rd 201 as spare parts to dip into if needed.
Paul -- Paul R. Hazelton, PhD Viral Gastroenteritis Study Group University of Manitoba Department of Medical Microbiology 511 Basic Medical Sciences Building 745 Bannatyne Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 4, 20 -- From paul_hazelton-at-umanitoba.ca Fri Sep 9 14:29:23 2011 4, 20 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p89JTNXQ017402 4, 20 -- for {microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 14:29:23 -0500 4, 20 -- Received: from [192.168.100.103] (wnpgmb016qw-ds01-216-100.dynamic.mtsallstream.net [207.161.216.100]) 4, 20 -- (user=hazeltn mech=LOGIN bits=0) 4, 20 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id p89JTLtA001027; 4, 20 -- Fri, 9 Sep 2011 14:29:21 -0500 (CDT) 4, 20 -- Message-ID: {4E6A690D.7090202-at-umanitoba.ca} 4, 20 -- Date: Fri, 09 Sep 2011 14:29:17 -0500 4, 20 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 4, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.0; WOW64; rv:6.0.2) Gecko/20110902 Thunderbird/6.0.2 4, 20 -- MIME-Version: 1.0 4, 20 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 4, 20 -- Subject: Re: [Microscopy] Re: Used SEM cost 4, 20 -- References: {201109091555.p89Ftgka032216-at-ns.microscopy.com} 4, 20 -- In-Reply-To: {201109091555.p89Ftgka032216-at-ns.microscopy.com} 4, 20 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 20 -- Content-Transfer-Encoding: 7bit 4, 20 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jbjasinski-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jbjasinski-at-gmail.com Name: Jacek Jasinski
Organization: Conn Center for Renewable Energy Research, University of Louisville
Title-Subject: [Filtered] Materials Characterization Post-Doctoral Fellow Position
Message: The Conn Center for Renewable Energy Research at the University of Louisville has an immediate opening for a two-year post-doctoral position for a person with expertise and demonstrated hands-on experience in materials characterization techniques, most notably, in TEM. The successful candidate will work on projects related to materials characterization/development with UofL faculty and students, as well as with external academic and industrial collaborators and customers. On a regular basis, he or she will be involved in data acquisition, analysis, and technical report/publication preparation. Other duties may include equipment maintenance, calibration, and user training.
Requirements:
Applicants who meet the following requirements will be considered:
1. A PhD with 2-3 years post-doctoral experience, or a recent PhD, in material science, chemical engineering, condensed matter physics, or a closely related subject area. 2. Strong academic background and hands-on experience in transmission electron microscopy of materials. Demonstrated expertise in at least some of the TEM-related techniques including HRTEM, electron diffraction, STEM, EDX, EELS, EFTEM, or in situ TEM, are required. 3. Good oral and written communication skills. 4. Good judgment, clear sense of purpose, and accountability.
Desirable is also hands-on research experience in one or more of the following areas:
1. Surface science and UHV techniques including XPS, Auger Spectroscopy and UPS. 2. Other materials characterization techniques such as XRD and SEM. 3. Laser-spectroscopic techniques including micro-Raman, PL and FTIR.
Salary: Negotiable based on the level of experience and expertise.
Interested candidates should contact:
Dr. Jacek Jasinski Theme Leader Materials Characterization Conn Center for Renewable Energy Research University of Louisville Louisville, KY 40292 Tel: (502) 852-6338 E-mail: jbjasinski-at-gmail.com (or jacek.jasinski-at-louisville.edu)
As with anything else, price mainly depends on how badly seller needs to get rid of it and how desperately buyer wants it "right now." Flea-market psychology aside - if we are talking about basic SEM without bells and whistles 10 years of age, then IMHO:
If it is not operational (regardless of the reasons why and claims about its condition) then the price is about... outrageous;
If it is operational but not currently under OEM (or OEM-like) service and/or does not fully conform to original specifications - the price is about x3 too high;
If it is fully serviced and conforms to original specifications - the price is about x2 too high;
If it was fully rebuilt to like-new condition by the OEM or reputable service provider, fully conforms to original specifications, and comes with 1 year parts and labor warranty, then the price is about right.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/9/2011 9:49 AM, DusevichV-at-umkc.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } Do you think price of $60,000 for 10 years old VP SEM (tungsten, Windows NT, turbomolecular pump, 100 mm X travel) is right? } What can cost 10 years old EDS system (LN, basic software, no fancy staff)? } } Thank you, } } Vladimir } } } ==============================Original Headers============================== } 5, 29 -- From DusevichV-at-umkc.edu Fri Sep 9 08:47:44 2011 } 5, 29 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p89DliG4026714 } 5, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Sep 2011 08:47:44 -0500 } 5, 29 -- X-IronPort-Anti-Spam-Filtered: true } 5, 29 -- X-IronPort-Anti-Spam-Result: Al4HAIUYak6Dl3nN/2dsb2JhbABBoVSGO3iBUwEFOk8CAT4QMhcBDQEBBBsYtlmIf4YOYASHbZ0D } 5, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) } 5, 29 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 09 Sep 2011 08:47:42 -0500 } 5, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.228]) by } 5, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Fri, 9 Sep 2011 } 5, 29 -- 08:47:42 -0500 } 5, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} } 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 5, 29 -- Date: Fri, 9 Sep 2011 08:47:41 -0500 } 5, 29 -- Subject: Used SEM cost } 5, 29 -- Thread-Topic: Used SEM cost } 5, 29 -- Thread-Index: AcxHsc69Hu/XiVO2SSy9af3ZJPKzGQnRRGbA } 5, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3B386A06E6A-at-UM-EMAIL06.um.umsystem.edu} } 5, 29 -- References: {201107211413.p6LEDwfL026098-at-ns.microscopy.com} } 5, 29 -- In-Reply-To: {201107211413.p6LEDwfL026098-at-ns.microscopy.com} } 5, 29 -- Accept-Language: en-US } 5, 29 -- Content-Language: en-US } 5, 29 -- X-MS-Has-Attach: } 5, 29 -- X-MS-TNEF-Correlator: } 5, 29 -- acceptlanguage: en-US } 5, 29 -- Content-Type: text/plain; charset="us-ascii" } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- Content-Transfer-Encoding: 8bit } 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p89DliG4026714 } ==============================End of - Headers============================== }
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tamavlyutov-at-wisc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tamavlyutov-at-wisc.edu Name: Timur Mavlyutov
Organization: University of Wisconsin
Title-Subject: [Filtered] 3-Dimensional reconstruction at EM level
Message: I want to make 3-dimensional reconstraction of biological sample (part of the neuron about 5 micron deep) at EM level. I know that I need to generate about 50 serial sections for it, all 100 nm thick. But what then? What program should I use, how to position images and to make final reconstruction?
Users at our unit have used Reconstruct for this purpose: http://synapses.clm.utexas.edu/tools/reconstruct/reconstruct.stm
Regards,
Ben
-- Research Support Manager MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271649 {http://mrcanu.pharm.ox.ac.uk/}
On 10/09/2011 16:56, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 30 -- From ben.micklem-at-pharm.ox.ac.uk Mon Sep 12 03:57:17 2011 13, 30 -- Received: from relay8.mail.ox.ac.uk (relay8.mail.ox.ac.uk [129.67.1.171]) 13, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8C8vGpi024605 13, 30 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 03:57:17 -0500 13, 30 -- Received: from smtp4.nexus.ox.ac.uk ([163.1.154.138] helo=exht04.ad.oak.ox.ac.uk) 13, 30 -- by relay8.mail.ox.ac.uk with esmtp (Exim 4.75) 13, 30 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 13, 30 -- id 1R32KK-0003uN-QW 13, 30 -- for microscopy-at-microscopy.com; Mon, 12 Sep 2011 09:57:16 +0100 13, 30 -- Received: from EXMBX04.ad.oak.ox.ac.uk ([169.254.1.79]) by 13, 30 -- exht04.ad.oak.ox.ac.uk ([163.1.154.55]) with mapi; Mon, 12 Sep 2011 09:57:16 13, 30 -- +0100 13, 30 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 13, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 13, 30 -- Date: Mon, 12 Sep 2011 09:57:15 +0100 13, 30 -- Subject: Re: [Microscopy] viaWWW:3-Dimensional reconstruction at EM level 13, 30 -- Thread-Topic: [Microscopy] viaWWW:3-Dimensional reconstruction at EM level 13, 30 -- Thread-Index: AcxxKfhtBe6h+QC1S9ugP25pfrIVrg== 13, 30 -- Message-ID: {CA9386E5.15B02%ben.micklem-at-pharm.ox.ac.uk} 13, 30 -- In-Reply-To: {201109101556.p8AFuwmc000940-at-ns.microscopy.com} 13, 30 -- Accept-Language: en-US, en-GB 13, 30 -- Content-Language: en-US 13, 30 -- X-MS-Has-Attach: 13, 30 -- X-MS-TNEF-Correlator: 13, 30 -- user-agent: Microsoft-MacOutlook/14.12.0.110505 13, 30 -- acceptlanguage: en-US, en-GB 13, 30 -- Content-Type: text/plain; charset="us-ascii" 13, 30 -- MIME-Version: 1.0 13, 30 -- Content-Transfer-Encoding: 8bit 13, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8C8vGpi024605 ==============================End of - Headers==============================
Andrews Dyson and Keown back in 1971 had lots of diffraction patterns to do with steels. Book is called The interpretation of diffraction patterns, good luck finding it.
If you've got the samples then you should be able to index the ring patterns from these known structures.
Cheers Rob
On 9/12/2011 11:38 AM, vic555-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello, } } Does anyone know (or refer me to literature) the lattice parameter for } Austenite, Martensite and Cementite for 1% steel quenched and tempered? } } I am trying to solve the SAD ring patterns for these structures. } } Thanks } Vikram } } ==============================Original Headers============================== } 4, 24 -- From vic555-at-gmail.com Mon Sep 12 10:31:45 2011 } 4, 24 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) } 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8CFVjw7031210 } 4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 10:31:45 -0500 } 4, 24 -- Received: by vwm42 with SMTP id 42so4729843vwm.0 } 4, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 08:31:44 -0700 (PDT) } 4, 24 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 4, 24 -- d=gmail.com; s=gamma; } 4, 24 -- h=mime-version:reply-to:date:message-id:subject:from:to:content-type; } 4, 24 -- bh=M2+vUuGKx7aAMFydeBj7T/q0f3vH1cgiQsjbH1IiV5E=; } 4, 24 -- b=D9MMskTBH4MgXGFRgT/eN7kMzcbNlvR/Plfu07KrqFDAkFqiG6mvm2TGbgMuB/q5d2 } 4, 24 -- bJOu1Zjp0g7lHbWHnsbIKddsdwmN9KFQ6pFUUurfk/gT8CHp6MB7cqPZtWwOw2xWAVtK } 4, 24 -- e/agABvBH+sHBwiTGLTQ+e+A/yKVieHzinSaY= } 4, 24 -- MIME-Version: 1.0 } 4, 24 -- Received: by 10.52.114.68 with SMTP id je4mr155920vdb.232.1315841504782; Mon, } 4, 24 -- 12 Sep 2011 08:31:44 -0700 (PDT) } 4, 24 -- Received: by 10.220.100.70 with HTTP; Mon, 12 Sep 2011 08:31:44 -0700 (PDT) } 4, 24 -- Reply-To: vic555-at-gmail.com } 4, 24 -- Date: Mon, 12 Sep 2011 11:31:44 -0400 } 4, 24 -- Message-ID: {CAM+LW=TV1fSwo7Bs2jBJD_wA_zAp4h0SczX+6faMt8gDOzSoDw-at-mail.gmail.com} } 4, 24 -- Subject: Lattice Parameter for Steel Phases } 4, 24 -- From: Vikram Bedekar {vic555-at-gmail.com} } 4, 24 -- To: Microscopy-at-microscopy.com } 4, 24 -- Content-Type: text/plain; charset=ISO-8859-1 } ==============================End of - Headers==============================
Two methods come to mind: polarized light microscopy (PLM) and electron backscatter diffraction (EBSD). It all depends on what you have access to and the particle size of your samples. Bigger particles should be easy to differentiate by PLM (uniaxial calcite vs. biaxial aragonite and refractive index differences), smaller (or larger) particles could be done by EBSD.
XRD might still be possible with a zero background sample holder if you have access to one.
Hope this helps.
Best, Bryan
-- ___________________________________________________________________________ Bryan R. Bandli
Research Instrumentation Laboratory Manager University of Minnesota, Duluth 229 Heller Hall 1114 Kirby Dr. Duluth, MN 55812 218-726-7362 ==================================================================
==============================Original Headers============================== 11, 32 -- From bbandli-at-d.umn.edu Mon Sep 12 11:54:06 2011 11, 32 -- Received: from mx0.d.umn.edu (mx0.d.umn.edu [131.212.109.42]) 11, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8CGs63S032719 11, 32 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 11:54:06 -0500 11, 32 -- Received: from mxr1.d.umn.edu (mxr1.d.umn.edu [131.212.109.92]) 11, 32 -- by mx0.d.umn.edu (8.13.8/8.13.8) with ESMTP id p8CGrwuZ006213 11, 32 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 11:53:58 -0500 (CDT) 11, 32 -- Received: from mxv2.d.umn.edu (mxv2.d.umn.edu [131.212.109.136]) 11, 32 -- by mxr1.d.umn.edu (8.13.8/8.13.8) with ESMTP id p8CGrwTF013567 11, 32 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 11:53:58 -0500 (CDT) 11, 32 -- Received: from mx4-g.d.umn.edu (mx4-g.d.umn.edu [131.212.109.174]) 11, 32 -- by mxv2.d.umn.edu (8.13.8/8.13.8) with ESMTP id p8CGrvIv004756 11, 32 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 11:53:58 -0500 (CDT) 11, 32 -- Received: from mail-yx0-f176.google.com (mail-yx0-f176.google.com [209.85.213.176]) 11, 32 -- by mx4-g.d.umn.edu (8.13.8/8.13.8) with ESMTP id p8CGr4dY007904 11, 32 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 11:53:22 -0500 (CDT) 11, 32 -- Received: by mail-yx0-f176.google.com with SMTP id 22so419211yxn.21 11, 32 -- for {microscopy-at-microscopy.com} ; Mon, 12 Sep 2011 09:53:22 -0700 (PDT) 11, 32 -- MIME-Version: 1.0 11, 32 -- Received: by 10.42.136.2 with SMTP id r2mr1183288ict.524.1315846401624; Mon, 11, 32 -- 12 Sep 2011 09:53:21 -0700 (PDT) 11, 32 -- Received: by 10.42.241.134 with HTTP; Mon, 12 Sep 2011 09:53:21 -0700 (PDT) 11, 32 -- Date: Mon, 12 Sep 2011 11:53:21 -0500 11, 32 -- Message-ID: {CAP4dfZVVdw7+9Qt6WX9NBFoF-FHxf1=DL1to-in=gBAmxhjZbw-at-mail.gmail.com} 11, 32 -- Subject: Re: Measuring Aragonite/Calcite ratios 11, 32 -- From: Bryan Bandli {bbandli-at-d.umn.edu} 11, 32 -- To: microscopy-at-microscopy.com 11, 32 -- Content-Type: text/plain; charset=ISO-8859-1 11, 32 -- X-Virus-Scanned: clamav-milter 0.96.5 at mxv2.d.umn.edu 11, 32 -- X-Virus-Status: Clean 11, 32 -- Content-Transfer-Encoding: 8bit 11, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8CGs63S032719 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: UI
Title-Subject: [Filtered] Olympus DP70 Camera Software/Drivers
Message: We have an Olympus DP 70 camera mounted on an Olympus BX 51 Fluorescence PLM (inherited from a former faculty). The supporting computer had to have a complete system rebuild and we cannot find the original software disks. Bottomline: we cannot get the camera to communicate with the computer (currently running Win XP). Would anyone know of a source for drivers or supporting software? Any assistance would be greatly appreciated!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: RamChandra
Organization: Haldor Topsoe
Title-Subject: [Filtered] EDS dectector- time to cool down
Message: Hi all,
We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million counts just after we add liquid N2 with the detector retracted and it takes almost two days to come down to around 10. what could be the possible reason for this? I think it usually it takes only about two hours for the crystal to cool down. I dont think there is any ice in the dewar.
It sounds like you are letting your detector come to room temp between uses. It is more likely to develop ice-buildups in this scenario. I would verify that you really do not have ice inside the dewar since that is the most likely cause of your extended cool down time.
Another possibility is that the cooling-connection (contact) between the LN2 and the detector has become loose.
John Bozzola
On Tue, Sep 13, 2011 at 8:26 AM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America } To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at  http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please  copy  both } rct204-at-gmail.com as well as  the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rct204-at-gmail.com Name: RamChandra } } Organization: Haldor Topsoe } } Title-Subject: [Filtered] EDS dectector- time to cool down }
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 6, 20 -- From bozzola-at-siu.edu Tue Sep 13 09:34:27 2011 6, 20 -- Received: from mail-ey0-f169.google.com (mail-ey0-f169.google.com [209.85.215.169]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DEYQ2M009812 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 09:34:27 -0500 6, 20 -- Received: by eye22 with SMTP id 22so386579eye.0 6, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 07:34:25 -0700 (PDT) 6, 20 -- MIME-Version: 1.0 6, 20 -- Received: by 10.52.175.230 with SMTP id cd6mr37903vdc.251.1315924463314; Tue, 6, 20 -- 13 Sep 2011 07:34:23 -0700 (PDT) 6, 20 -- Received: by 10.52.112.134 with HTTP; Tue, 13 Sep 2011 07:34:23 -0700 (PDT) 6, 20 -- In-Reply-To: {201109131326.p8DDQYke009275-at-ns.microscopy.com} 6, 20 -- References: {201109131326.p8DDQYke009275-at-ns.microscopy.com} 6, 20 -- Date: Tue, 13 Sep 2011 09:34:23 -0500 6, 20 -- Message-ID: {CAKeCJ9yM7XHGbztjZbgt3xU=yzMem1EKNcV8Owd7b6CyUGkgwg-at-mail.gmail.com} 6, 20 -- Subject: Re: [Microscopy] viaWWW:EDS dectector- time to cool down 6, 20 -- From: John Bozzola {bozzola-at-siu.edu} 6, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} , rct204-at-gmail.com 6, 20 -- Content-Type: text/plain; charset=UTF-8 6, 20 -- Content-Transfer-Encoding: 8bit 6, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DEYQ2M009812 ==============================End of - Headers==============================
It sounds like you are applying voltage to the detector as soon as you have introduced the LN2. I don't know that is a good idea. I was of the impression that the Li can quickly diffuse out of the crystal if voltage is applied while the crystal is warm. I think you would want to let the crystal cool for some time before applying power. Once the Li is gone, the crystal would need to be replaced. I would like someone else with more experience to comment on that. We keep our detectors cold 24-7.
You don't mention what brand of system you have. We have an older Oxford ISIS. Its normal behavior is to register hundreds if not thousands of counts when the software is first started as the various parameters are optimized internally. After about 5 minutes, the count had stabilized around 200-300 cps with the beam off. I would not expect it to get down to 10 cps since there is always a strobe peak present for reference.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, September 13, 2011 8:26 AM To: wesaia-at-iastate.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: RamChandra
Organization: Haldor Topsoe
Title-Subject: [Filtered] EDS dectector- time to cool down
Message: Hi all,
We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million counts just after we add liquid N2 with the detector retracted and it takes almost two days to come down to around 10. what could be the possible reason for this? I think it usually it takes only about two hours for the crystal to cool down. I dont think there is any ice in the dewar.
My experience with LN2 cooled EDS is to waited at least an hour after the dewar has stopped simmering off LN2. Two hours sounds about right from room temperature. Let me agree with the other responders. It's better to keep the Si-Li detector cold all the time. I will, on 4 day week-ends turn off the EDS unplug it if necessary, and allow it to warm up to room temp under vacuum. I want to help evaporate the pump oil which collects on the probe. But I give it plenty of time to cool down. With out the EDS, well, my SEM is just a big camera so I protect it with caution.
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Tuesday, September 13, 2011 11:31 AM To: Frank Karl
It sounds like you are applying voltage to the detector as soon as you have introduced the LN2. I don't know that is a good idea. I was of the impression that the Li can quickly diffuse out of the crystal if voltage is applied while the crystal is warm. I think you would want to let the crystal cool for some time before applying power. Once the Li is gone, the crystal would need to be replaced. I would like someone else with more experience to comment on that. We keep our detectors cold 24-7.
You don't mention what brand of system you have. We have an older Oxford ISIS. Its normal behavior is to register hundreds if not thousands of counts when the software is first started as the various parameters are optimized internally. After about 5 minutes, the count had stabilized around 200-300 cps with the beam off. I would not expect it to get down to 10 cps since there is always a strobe peak present for reference.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, September 13, 2011 8:26 AM To: wesaia-at-iastate.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: RamChandra
Organization: Haldor Topsoe
Title-Subject: [Filtered] EDS dectector- time to cool down
Message: Hi all,
We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million counts just after we add liquid N2 with the detector retracted and it takes almost two days to come down to around 10. what could be the possible reason for this? I think it usually it takes only about two hours for the crystal to cool down. I dont think there is any ice in the dewar.
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 27, 26 -- From frank_karl-at-ardl.com Tue Sep 13 10:52:32 2011 27, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DFqV94013266 27, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 10:52:31 -0500 27, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 27, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Tue, 13 27, 26 -- Sep 2011 11:52:30 -0400 27, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 27, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 27, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 27, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 27, 26 -- Date: Tue, 13 Sep 2011 11:52:29 -0400 27, 26 -- Subject: RE: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 27, 26 -- Thread-Topic: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 27, 26 -- Thread-Index: AcxyKi0NrTxQZ3sBQVKhvj8dVpv6dwAAb3yw 27, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF7C-at-exchange2k7.ad.ardl.com} 27, 26 -- In-Reply-To: {201109131531.p8DFV62S011533-at-ns.microscopy.com} 27, 26 -- Accept-Language: en-US 27, 26 -- Content-Language: en-US 27, 26 -- X-MS-Has-Attach: 27, 26 -- X-MS-TNEF-Correlator: 27, 26 -- acceptlanguage: en-US 27, 26 -- Content-Type: text/plain; charset="iso-8859-1" 27, 26 -- MIME-Version: 1.0 27, 26 -- Content-Transfer-Encoding: 8bit 27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DFqV94013266 ==============================End of - Headers==============================
If Warren is correct and you've been turning on the bias before at least a 2 hour cool-down (my more conservative customers prefer overnight), you probably need a detector rebuild because the Li has been pulled out of the Si and also, the optically coupled FET may be dead. It is also cooled.
Ken Converse, owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: Tuesday, September 13, 2011 11:54 AM To: kenconverse-at-qualityimages.biz
My experience with LN2 cooled EDS is to waited at least an hour after the dewar has stopped simmering off LN2. Two hours sounds about right from room temperature. Let me agree with the other responders. It's better to keep the Si-Li detector cold all the time. I will, on 4 day week-ends turn off the EDS unplug it if necessary, and allow it to warm up to room temp under vacuum. I want to help evaporate the pump oil which collects on the probe. But I give it plenty of time to cool down. With out the EDS, well, my SEM is just a big camera so I protect it with caution.
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Tuesday, September 13, 2011 11:31 AM To: Frank Karl
It sounds like you are applying voltage to the detector as soon as you have introduced the LN2. I don't know that is a good idea. I was of the impression that the Li can quickly diffuse out of the crystal if voltage is applied while the crystal is warm. I think you would want to let the crystal cool for some time before applying power. Once the Li is gone, the crystal would need to be replaced. I would like someone else with more experience to comment on that. We keep our detectors cold 24-7.
You don't mention what brand of system you have. We have an older Oxford ISIS. Its normal behavior is to register hundreds if not thousands of counts when the software is first started as the various parameters are optimized internally. After about 5 minutes, the count had stabilized around 200-300 cps with the beam off. I would not expect it to get down to 10 cps since there is always a strobe peak present for reference.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, September 13, 2011 8:26 AM To: wesaia-at-iastate.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: RamChandra
Organization: Haldor Topsoe
Title-Subject: [Filtered] EDS dectector- time to cool down
Message: Hi all,
We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million counts just after we add liquid N2 with the detector retracted and it takes almost two days to come down to around 10. what could be the possible reason for this? I think it usually it takes only about two hours for the crystal to cool down. I dont think there is any ice in the dewar.
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 27, 26 -- From frank_karl-at-ardl.com Tue Sep 13 10:52:32 2011 27, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DFqV94013266 27, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 10:52:31 -0500 27, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 27, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Tue, 13 27, 26 -- Sep 2011 11:52:30 -0400 27, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 27, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 27, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 27, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 27, 26 -- Date: Tue, 13 Sep 2011 11:52:29 -0400 27, 26 -- Subject: RE: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 27, 26 -- Thread-Topic: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 27, 26 -- Thread-Index: AcxyKi0NrTxQZ3sBQVKhvj8dVpv6dwAAb3yw 27, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF7C-at-exchange2k7.ad.ardl.com} 27, 26 -- In-Reply-To: {201109131531.p8DFV62S011533-at-ns.microscopy.com} 27, 26 -- Accept-Language: en-US 27, 26 -- Content-Language: en-US 27, 26 -- X-MS-Has-Attach: 27, 26 -- X-MS-TNEF-Correlator: 27, 26 -- acceptlanguage: en-US 27, 26 -- Content-Type: text/plain; charset="iso-8859-1" 27, 26 -- MIME-Version: 1.0 27, 26 -- Content-Transfer-Encoding: 8bit 27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DFqV94013266 ==============================End of - Headers============================== ----- No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1392 / Virus Database: 1520/3894 - Release Date: 09/13/11
==============================Original Headers============================== 37, 28 -- From kenconverse-at-qualityimages.biz Tue Sep 13 11:14:04 2011 37, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.120]) 37, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DGE3V6030385 37, 28 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 11:14:04 -0500 37, 28 -- X-Authority-Analysis: v=1.1 cv=clOQl2Kon9ihL0vqe5S6shCOWnQ8eDPHG/StQ1omz74= c=1 sm=0 a=aKVhv7_vg2EA:10 a=kj9zAlcOel0A:10 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=YdczCmdkAAAA:8 a=Zx37jsudAAAA:8 a=pGLkceISAAAA:8 a=pzmDz54eAAAA:8 a=jU2bWg6BAAAA:8 a=oCcaPWc0AAAA:8 a=27SjLU4nyUcWpFE7VEoA:9 a=yp7T2u6gQtEBVTAyGvwA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=Joj3jlGTlb8A:10 a=anj8_u9ehFoA:10 a=C3J4oLNe8gwA:10 a=Bd5jmW48H-gA:10 a=RyjLG6jaqdoA:10 a=AO_5y2s0I30A:10 a=3_2eAsHTqJoA:10 a=bsRvvyHUn3IA:10 a=C6KWKcr4_fMA:10 a=G91thX30KR8A:10 a=MSl-tDqOz04A:10 a=H8a8glrR3zcvCdK4:21 a=kXOEOTqCJZxqck61:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 37, 28 -- X-Cloudmark-Score: 0 37, 28 -- X-Originating-IP: 72.224.149.57 37, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2874] helo=Ken) 37, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 37, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 37, 28 -- id 61/16-15370-B418F6E4; Tue, 13 Sep 2011 16:14:03 +0000 37, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 37, 28 -- To: "MSA Listserver" {microscopy-at-microscopy.com} 37, 28 -- Subject: RE: [Microscopy] viaWWW:EDS dectector- time to cool down 37, 28 -- Date: Tue, 13 Sep 2011 12:13:59 -0400 37, 28 -- Message-ID: {61AF9AB0F0464813829150309E758263-at-Ken} 37, 28 -- MIME-Version: 1.0 37, 28 -- Content-Type: text/plain; 37, 28 -- charset="us-ascii" 37, 28 -- X-Priority: 3 (Normal) 37, 28 -- X-MSMail-Priority: Normal 37, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 37, 28 -- Importance: Normal 37, 28 -- Thread-Index: AcxyLWMuRBAU3lpERIq83TB4si/MDwAAdk0g 37, 28 -- In-Reply-To: {201109131554.p8DFsEov016740-at-ns.microscopy.com} 37, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 37, 28 -- Content-Transfer-Encoding: 8bit 37, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DGE3V6030385 ==============================End of - Headers==============================
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, September 13, 2011 8:37 AM To: Joachim Siegmund
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html ------------------------------------------------------------------------ --- Remember this posting is most likely not from a Subscriber, so when replying please copy both tomw-at-uidaho.edu as well as the MIcroscopy Listserver ------------------------------------------------------------------------ ---
Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: UI
Title-Subject: [Filtered] Olympus DP70 Camera Software/Drivers
Message: We have an Olympus DP 70 camera mounted on an Olympus BX 51 Fluorescence PLM (inherited from a former faculty). The supporting computer had to have a complete system rebuild and we cannot find the original software disks. Bottomline: we cannot get the camera to communicate with the computer (currently running Win XP). Would anyone know of a source for drivers or supporting software? Any assistance would be greatly appreciated!
Sounds like one of two things. 1. Loss of Dewar vacuum? 2. Perforated window.
Due to inexperience, I am unaware of the other 'ten' alternatives.
Cheers and best wishes,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state. 1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008. 2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, September 13, 2011 9:38 AM To: Monson, Frederick
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: RamChandra
Organization: Haldor Topsoe
Title-Subject: [Filtered] EDS dectector- time to cool down
Message: Hi all,
We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million counts just after we add liquid N2 with the detector retracted and it takes almost two days to come down to around 10. what could be the possible reason for this? I think it usually it takes only about two hours for the crystal to cool down. I dont think there is any ice in the dewar.
Hi everyone, I am looking for a substitute for Nanoplast resin. I need something relatively water soluble for working with clay suspensions. THanks John Shields Univ. of Georgia Athens
==============================Original Headers============================== 2, 44 -- From jpshield-at-uga.edu Tue Sep 13 13:24:56 2011 2, 44 -- Received: from AM1EHSOBE001.bigfish.com (am1ehsobe001.messaging.microsoft.com [213.199.154.204]) 2, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DIOuiE020910 2, 44 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 13:24:56 -0500 2, 44 -- Received: from mail114-am1-R.bigfish.com (10.3.201.244) by 2, 44 -- AM1EHSOBE001.bigfish.com (10.3.204.21) with Microsoft SMTP Server id 2, 44 -- 14.1.225.22; Tue, 13 Sep 2011 18:24:55 +0000 2, 44 -- Received: from mail114-am1 (localhost.localdomain [127.0.0.1]) by 2, 44 -- mail114-am1-R.bigfish.com (Postfix) with ESMTP id 8C61E1158345 for 2, 44 -- {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 18:24:55 +0000 (UTC) 2, 44 -- X-SpamScore: -7 2, 44 -- X-BigFish: PS-7(zz14ffOzz1202hzzz2fh2a8h668h839h944h65h) 2, 44 -- X-Spam-TCS-SCL: 4:0 2, 44 -- X-Forefront-Antispam-Report: CIP:207.46.198.81;KIP:(null);UIP:(null);IPVD:NLI;H:CH1PRD0202HT004.namprd02.prod.outlook.com;RD:none;EFVD:NLI 2, 44 -- Received-SPF: pass (mail114-am1: domain of uga.edu designates 207.46.198.81 as permitted sender) client-ip=207.46.198.81; envelope-from=jpshield-at-uga.edu; helo=CH1PRD0202HT004.namprd02.prod.outlook.com ;.outlook.com ; 2, 44 -- Received: from mail114-am1 (localhost.localdomain [127.0.0.1]) by mail114-am1 2, 44 -- (MessageSwitch) id 1315938294387894_31357; Tue, 13 Sep 2011 18:24:54 +0000 2, 44 -- (UTC) 2, 44 -- Received: from AM1EHSMHS016.bigfish.com (unknown [10.3.201.246]) by 2, 44 -- mail114-am1.bigfish.com (Postfix) with ESMTP id 51FDE1828046 for 2, 44 -- {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 18:24:54 +0000 (UTC) 2, 44 -- Received: from CH1PRD0202HT004.namprd02.prod.outlook.com (207.46.198.81) by 2, 44 -- AM1EHSMHS016.bigfish.com (10.3.207.154) with Microsoft SMTP Server (TLS) id 2, 44 -- 14.1.225.22; Tue, 13 Sep 2011 18:24:53 +0000 2, 44 -- Received: from CH1PRD0202MB123.namprd02.prod.outlook.com ([169.254.2.74]) by 2, 44 -- CH1PRD0202HT004.namprd02.prod.outlook.com ([10.42.82.103]) with mapi id 2, 44 -- 14.01.0225.067; Tue, 13 Sep 2011 18:24:52 +0000 2, 44 -- From: John P Shields {jpshield-at-uga.edu} 2, 44 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 44 -- Subject: nanoplast replacement 2, 44 -- Thread-Topic: nanoplast replacement 2, 44 -- Thread-Index: AcxyQdkdZKmsmsIUT4S8EN9C02yyew== 2, 44 -- Date: Tue, 13 Sep 2011 18:24:52 +0000 2, 44 -- Message-ID: {9696A79BF7D6014182BDD7047CDD03A90A9559D4-at-CH1PRD0202MB123.namprd02.prod.outlook.com} 2, 44 -- Accept-Language: en-US 2, 44 -- Content-Language: en-US 2, 44 -- X-MS-Has-Attach: 2, 44 -- X-MS-TNEF-Correlator: 2, 44 -- x-originating-ip: [128.192.63.15] 2, 44 -- Content-Type: text/plain; charset="us-ascii" 2, 44 -- MIME-Version: 1.0 2, 44 -- X-OriginatorOrg: uga.edu 2, 44 -- Content-Transfer-Encoding: 8bit 2, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DIOuiE020910 ==============================End of - Headers==============================
I agree completely about it being better to keep the LN2 in there all the time.
Warming up can degrade the internal vacuum, especially if the detector vacuum system is not well designed.
I wrecked a detector once by warming it up over Xmas, after being assured by the manufacturer that is would be OK. On recooling (reapplying the bias after a day of recooling), the resolution had degraded appreciably. I rechecked with the manufacturer who then said that was to be expected!
I can't post that manufacturer's name here, but it is available on request. I would advise against buying a detector from that manufacturer.
cheers Ritchie Sims The University of Auckland
________________________________________ X-from: frank_karl-at-ardl.com [frank_karl-at-ardl.com] Sent: 14 September 2011 03:53 To: Ritchie Sims
My experience with LN2 cooled EDS is to waited at least an hour after the dewar has stopped simmering off LN2. Two hours sounds about right from room temperature. Let me agree with the other responders. It's better to keep the Si-Li detector cold all the time. I will, on 4 day week-ends turn off the EDS unplug it if necessary, and allow it to warm up to room temp under vacuum. I want to help evaporate the pump oil which collects on the probe. But I give it plenty of time to cool down. With out the EDS, well, my SEM is just a big camera so I protect it with caution.
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Tuesday, September 13, 2011 11:31 AM To: Frank Karl
It sounds like you are applying voltage to the detector as soon as you have introduced the LN2. I don't know that is a good idea. I was of the impression that the Li can quickly diffuse out of the crystal if voltage is applied while the crystal is warm. I think you would want to let the crystal cool for some time before applying power. Once the Li is gone, the crystal would need to be replaced. I would like someone else with more experience to comment on that. We keep our detectors cold 24-7.
You don't mention what brand of system you have. We have an older Oxford ISIS. Its normal behavior is to register hundreds if not thousands of counts when the software is first started as the various parameters are optimized internally. After about 5 minutes, the count had stabilized around 200-300 cps with the beam off. I would not expect it to get down to 10 cps since there is always a strobe peak present for reference.
Warren Straszheim
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, September 13, 2011 8:26 AM To: wesaia-at-iastate.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rct204-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rct204-at-gmail.com Name: RamChandra
Organization: Haldor Topsoe
Title-Subject: [Filtered] EDS dectector- time to cool down
Message: Hi all,
We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million counts just after we add liquid N2 with the detector retracted and it takes almost two days to come down to around 10. what could be the possible reason for this? I think it usually it takes only about two hours for the crystal to cool down. I dont think there is any ice in the dewar.
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 27, 26 -- From frank_karl-at-ardl.com Tue Sep 13 10:52:32 2011 27, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DFqV94013266 27, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 10:52:31 -0500 27, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 27, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Tue, 13 27, 26 -- Sep 2011 11:52:30 -0400 27, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 27, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 27, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 27, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 27, 26 -- Date: Tue, 13 Sep 2011 11:52:29 -0400 27, 26 -- Subject: RE: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 27, 26 -- Thread-Topic: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 27, 26 -- Thread-Index: AcxyKi0NrTxQZ3sBQVKhvj8dVpv6dwAAb3yw 27, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF7C-at-exchange2k7.ad.ardl.com} 27, 26 -- In-Reply-To: {201109131531.p8DFV62S011533-at-ns.microscopy.com} 27, 26 -- Accept-Language: en-US 27, 26 -- Content-Language: en-US 27, 26 -- X-MS-Has-Attach: 27, 26 -- X-MS-TNEF-Correlator: 27, 26 -- acceptlanguage: en-US 27, 26 -- Content-Type: text/plain; charset="iso-8859-1" 27, 26 -- MIME-Version: 1.0 27, 26 -- Content-Transfer-Encoding: 8bit 27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DFqV94013266 ==============================End of - Headers==============================
I was just thinking that another side effect of warming and cooling the detector might be that the solid rod that runs down the length of the snout (For cooling purposes) will expand and contract with each cycle- does this expansion and contraction loosen the thermal connection between the rod and chip, or could it push the crystal up against the window?
--Justin A. Kraft
On Sep 13, 2011, at 2:31 PM, r.sims-at-auckland.ac.nz wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I agree completely about it being better to keep the LN2 in there all the time. } } Warming up can degrade the internal vacuum, especially if the detector vacuum system is not well designed. } } I wrecked a detector once by warming it up over Xmas, after being assured by the manufacturer that is would be OK. On recooling (reapplying the bias after a day of recooling), the resolution had degraded appreciably. I rechecked with the manufacturer who then said that was to be expected! } } I can't post that manufacturer's name here, but it is available on request. I would advise against buying a detector from that manufacturer. } } cheers } Ritchie Sims } The University of Auckland } } ________________________________________ } X-from: frank_karl-at-ardl.com [frank_karl-at-ardl.com] } Sent: 14 September 2011 03:53 } To: Ritchie Sims } Subject: [Microscopy] viaWWW:EDS dectector- time to cool down } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } My experience with LN2 cooled EDS is to waited at least an hour after the dewar has stopped simmering off LN2. Two hours sounds about right from room temperature. Let me agree with the other responders. It's better to keep the Si-Li detector cold all the time. I will, on 4 day week-ends turn off the EDS unplug it if necessary, and allow it to warm up to room temp under vacuum. I want to help evaporate the pump oil which collects on the probe. But I give it plenty of time to cool down. With out the EDS, well, my SEM is just a big camera so I protect it with caution. } } -----Original Message----- } X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] } Sent: Tuesday, September 13, 2011 11:31 AM } To: Frank Karl } Subject: [Microscopy] RE: viaWWW:EDS dectector- time to cool down } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } It sounds like you are applying voltage to the detector as soon as you have introduced the LN2. I don't know that is a good idea. I was of the impression that the Li can quickly diffuse out of the crystal if voltage is applied while the crystal is warm. I think you would want to let the crystal cool for some time before applying power. Once the Li is gone, the crystal would need to be replaced. I would like someone else with more experience to comment on that. We keep our detectors cold 24-7. } } You don't mention what brand of system you have. We have an older Oxford ISIS. Its normal behavior is to register hundreds if not thousands of counts when the software is first started as the various parameters are optimized internally. After about 5 minutes, the count had stabilized around 200-300 cps with the beam off. I would not expect it to get down to 10 cps since there is always a strobe peak present for reference. } } Warren Straszheim } } -----Original Message----- } X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Tuesday, September 13, 2011 8:26 AM } To: wesaia-at-iastate.edu } Subject: [Microscopy] viaWWW:EDS dectector- time to cool down } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } rct204-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: rct204-at-gmail.com Name: RamChandra } } Organization: Haldor Topsoe } } Title-Subject: [Filtered] EDS dectector- time to cool down } } Message: Hi all, } } We have a traditional Si-Li EDS detector on or CM200 microscope. We have just noticed that when the } we add liquid nitrogen the crystal takes almost two days to cool down. We observe half a million } counts just after we add liquid N2 with the detector retracted and it takes almost two days to come } down to around 10. what could be the possible reason for this? } I think it usually it takes only about two hours for the crystal to cool down. I dont think there } is any ice in the dewar. } } Any ideas? } } Login Host: 195.41.211.254 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Sep 13 08:24:56 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DDOuha007207 } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 08:24:56 -0500 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 48D9F40E3DA } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 08:24:56 -0500 (CDT) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id VLWd5AfDZFx8 for {microscopy-at-microscopy.com} ; } 10, 25 -- Tue, 13 Sep 2011 08:24:51 -0500 (CDT) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id 7192040E3CF } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 08:24:51 -0500 (CDT) } 10, 25 -- Message-ID: {4E6F59A3.80904-at-microscopy.com} } 10, 25 -- Date: Tue, 13 Sep 2011 08:24:51 -0500 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: rct204-at-gmail.com } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: viaWWW:EDS dectector- time to cool down } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 16, 31 -- From wesaia-at-iastate.edu Tue Sep 13 10:24:05 2011 } 16, 31 -- Received: from mailexch-2.iastate.edu (mailexch-2.iastate.edu [129.186.140.22]) } 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DFO5cc028148 } 16, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 10:24:05 -0500 } 16, 31 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702d.its.iastate.edu [10.10.87.141]) } 16, 31 -- by mailexch-2.iastate.edu (8.14.4/8.12.10) with ESMTP id p8DFO3D4005118; } 16, 31 -- Tue, 13 Sep 2011 10:24:04 -0500 } 16, 31 -- Received: from EXITS711.its.iastate.edu ([10.10.87.143]) by } 16, 31 -- EXITS702D.its.iastate.edu ([::1]) with mapi; Tue, 13 Sep 2011 10:23:57 -0500 } 16, 31 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} } 16, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 16, 31 -- CC: "rct204-at-gmail.com" {rct204-at-gmail.com} } 16, 31 -- Date: Tue, 13 Sep 2011 10:24:02 -0500 } 16, 31 -- Subject: RE: [Microscopy] viaWWW:EDS dectector- time to cool down } 16, 31 -- Thread-Topic: [Microscopy] viaWWW:EDS dectector- time to cool down } 16, 31 -- Thread-Index: AcxyGL27d2p9BH5IS+qVTsQkSC55JwADEt0w } 16, 31 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F1778C02CC3-at-EXITS711.its.iastate.edu} } 16, 31 -- References: {201109131326.p8DDQPxY008769-at-ns.microscopy.com} } 16, 31 -- In-Reply-To: {201109131326.p8DDQPxY008769-at-ns.microscopy.com} } 16, 31 -- Accept-Language: en-US } 16, 31 -- Content-Language: en-US } 16, 31 -- X-MS-Has-Attach: } 16, 31 -- X-MS-TNEF-Correlator: } 16, 31 -- acceptlanguage: en-US } 16, 31 -- Content-Type: text/plain; charset="us-ascii" } 16, 31 -- MIME-Version: 1.0 } 16, 31 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.9.13.151515 } 16, 31 -- X-ISUMailhub-test: Gauge=XXIII, Probability=23%, Report=' } 16, 31 -- RATWARE_RCVD_BONUS_SPC 2.0, TO_IN_SUBJECT 0.5, SUPERLONG_LINE 0.05, BODY_SIZE_5000_5999 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, DATE_TZ_NEG_0500 0, FROM_NAME_PHRASE 0, __ANY_URI 0, __BOUNCE_CHALLENGE_SUBJ 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __CP_URI_IN_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __FRAUD_BODY_WEBMAIL 0, __FRAUD_CONTACT_NAME 0, __FRAUD_WEBMAIL 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __STOCK_PHRASE_24 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_NS ' } 16, 31 -- Content-Transfer-Encoding: 8bit } 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DFO5cc028148 } ==============================End of - Headers============================== } } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } ==============================Original Headers============================== } 27, 26 -- From frank_karl-at-ardl.com Tue Sep 13 10:52:32 2011 } 27, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) } 27, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DFqV94013266 } 27, 26 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 10:52:31 -0500 } 27, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by } 27, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Tue, 13 } 27, 26 -- Sep 2011 11:52:30 -0400 } 27, 26 -- From: Frank Karl {frank_karl-at-ardl.com} } 27, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} } 27, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} } 27, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} } 27, 26 -- Date: Tue, 13 Sep 2011 11:52:29 -0400 } 27, 26 -- Subject: RE: [Microscopy] RE: viaWWW:EDS dectector- time to cool down } 27, 26 -- Thread-Topic: [Microscopy] RE: viaWWW:EDS dectector- time to cool down } 27, 26 -- Thread-Index: AcxyKi0NrTxQZ3sBQVKhvj8dVpv6dwAAb3yw } 27, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AF7C-at-exchange2k7.ad.ardl.com} } 27, 26 -- In-Reply-To: {201109131531.p8DFV62S011533-at-ns.microscopy.com} } 27, 26 -- Accept-Language: en-US } 27, 26 -- Content-Language: en-US } 27, 26 -- X-MS-Has-Attach: } 27, 26 -- X-MS-TNEF-Correlator: } 27, 26 -- acceptlanguage: en-US } 27, 26 -- Content-Type: text/plain; charset="iso-8859-1" } 27, 26 -- MIME-Version: 1.0 } 27, 26 -- Content-Transfer-Encoding: 8bit } 27, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DFqV94013266 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 36, 51 -- From r.sims-at-auckland.ac.nz Tue Sep 13 14:27:13 2011 } 36, 51 -- Received: from mx2-int.auckland.ac.nz (mx2-int.auckland.ac.nz [130.216.12.41]) } 36, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DJRCji005813 } 36, 51 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 14:27:12 -0500 } 36, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 36, 51 -- d=auckland.ac.nz; i=r.sims-at-auckland.ac.nz; q=dns/txt; } 36, 51 -- s=uoa; t=1315942033; x=1347478033; } 36, 51 -- h=from:to:subject:date:message-id:references:in-reply-to: } 36, 51 -- content-transfer-encoding:mime-version; } 36, 51 -- z=From:=20Ritchie=20Sims=20 {r.sims-at-auckland.ac.nz} |To:=20" } 36, 51 -- microscopy-at-microscopy.com"=20 {microscopy-at-microscopy.com} } 36, 51 -- |Subject:=20RE:=20[Microscopy]=20=20viaWWW:EDS=20dectecto } 36, 51 -- r-=20time=20to=20cool=20down|Date:=20Tue,=2013=20Sep=2020 } 36, 51 -- 11=2019:27:09=20+0000|Message-ID:=20 {65F9DBC3DFB6DE44BE76 } 36, 51 -- 95B82D539F1A1E2DA77A-at-uxcn10-3.UoA.auckland.ac.nz} } 36, 51 -- |References:=20 {201109131553.p8DFr8Ak014421-at-ns.microscopy } 36, 51 -- .com} |In-Reply-To:=20 {201109131553.p8DFr8Ak014421-at-ns.micr } 36, 51 -- oscopy.com} |Content-Transfer-Encoding:=20quoted-printable } 36, 51 -- |MIME-Version:=201.0; } 36, 51 -- bh=stYtK78kEBpKkmDATwT4fa+OJiUrpZOPnShSOUOjsOA=; } 36, 51 -- b=lLepGcV8dH56yg3a9jpJNnN93HVsVkJJ72r6k38FOZbBxGjkX+3L+CQk } 36, 51 -- LHnULZ2t6OAojDDXfAPUfIk2U2uRFzB9Nsfs9MOpXCds2WUuguTO41abT } 36, 51 -- 0HVtjXxMbDKpRa+H674xfr4cepmCkUwmvVqXUhGxIY1xijPGm8FeWHP8I } 36, 51 -- E=; } 36, 51 -- X-IronPort-AV: E=Sophos;i="4.68,375,1312113600"; } 36, 51 -- d="scan'208";a="83380159" } 36, 51 -- X-Ironport-HAT: MAIL-SERVERS - $RELAYED } 36, 51 -- X-Ironport-Source: 130.216.4.106 - Outgoing - Outgoing } 36, 51 -- Received: from uxchange10-fe2.itsys.auckland.ac.nz (HELO uxchange10-fe2.UoA.auckland.ac.nz) ([130.216.4.106]) } 36, 51 -- by mx2-int.auckland.ac.nz with ESMTP/TLS/AES128-SHA; 14 Sep 2011 07:27:11 +1200 } 36, 51 -- Received: from UXCN10-3.UoA.auckland.ac.nz ([169.254.3.245]) by } 36, 51 -- uxchange10-fe2.UoA.auckland.ac.nz ([130.216.4.106]) with mapi id } 36, 51 -- 14.01.0289.001; Wed, 14 Sep 2011 07:27:10 +1200 } 36, 51 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz} } 36, 51 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 36, 51 -- Subject: RE: [Microscopy] viaWWW:EDS dectector- time to cool down } 36, 51 -- Thread-Topic: [Microscopy] viaWWW:EDS dectector- time to cool down } 36, 51 -- Thread-Index: AQHMci0+WmuS2uAntk2Y7BRAt9inkpVLsDAt } 36, 51 -- Date: Tue, 13 Sep 2011 19:27:09 +0000 } 36, 51 -- Message-ID: {65F9DBC3DFB6DE44BE7695B82D539F1A1E2DA77A-at-uxcn10-3.UoA.auckland.ac.nz} } 36, 51 -- References: {201109131553.p8DFr8Ak014421-at-ns.microscopy.com} } 36, 51 -- In-Reply-To: {201109131553.p8DFr8Ak014421-at-ns.microscopy.com} } 36, 51 -- Accept-Language: en-GB, en-NZ, en-US } 36, 51 -- Content-Language: en-GB } 36, 51 -- X-MS-Has-Attach: } 36, 51 -- X-MS-TNEF-Correlator: } 36, 51 -- x-originating-ip: [130.216.11.249] } 36, 51 -- Content-Type: text/plain; charset="us-ascii" } 36, 51 -- MIME-Version: 1.0 } 36, 51 -- Content-Transfer-Encoding: 8bit } 36, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DJRCji005813 } ==============================End of - Headers==============================
==============================Original Headers============================== 7, 33 -- From kraftpiano-at-gmail.com Tue Sep 13 14:47:06 2011 7, 33 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DJl6V9022484 7, 33 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 14:47:06 -0500 7, 33 -- Received: by yxi19 with SMTP id 19so897460yxi.0 7, 33 -- for {microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 12:47:06 -0700 (PDT) 7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 33 -- d=gmail.com; s=gamma; 7, 33 -- h=subject:mime-version:content-type:from:in-reply-to:date:cc 7, 33 -- :content-transfer-encoding:message-id:references:to:x-mailer; 7, 33 -- bh=SX/SqcHpMee0BiUnhahg+xrYQampXqi0QmManduprTk=; 7, 33 -- b=pEkxbe7Pr4ZEdjl4xQjk5BK8UXU4YoYiJO8qbejiiNZUttr7hN67zQyiba01uKArRb 7, 33 -- NcshkSgOgqTSiH9cL0MhmQgEfw6j8eltbUiAYQQaj+eP4GnMsYpbfs3SgxJ0x+9P8n1Y 7, 33 -- Cl5NzNfOyNv27e8tcOjN9nEvfenFXifVywyEY= 7, 33 -- Received: by 10.236.145.10 with SMTP id o10mr36916920yhj.90.1315943225342; 7, 33 -- Tue, 13 Sep 2011 12:47:05 -0700 (PDT) 7, 33 -- Received: from [192.168.15.101] (c-98-195-153-235.hsd1.tx.comcast.net [98.195.153.235]) 7, 33 -- by mx.google.com with ESMTPS id f14sm3985660ani.15.2011.09.13.12.47.02 7, 33 -- (version=TLSv1/SSLv3 cipher=OTHER); 7, 33 -- Tue, 13 Sep 2011 12:47:03 -0700 (PDT) 7, 33 -- Subject: Re: [Microscopy] RE: viaWWW:EDS dectector- time to cool down 7, 33 -- Mime-Version: 1.0 (Apple Message framework v1244.3) 7, 33 -- Content-Type: text/plain; charset=us-ascii 7, 33 -- From: Justin Kraft {kraftpiano-at-gmail.com} 7, 33 -- In-Reply-To: {201109131931.p8DJVd8v013372-at-ns.microscopy.com} 7, 33 -- Date: Tue, 13 Sep 2011 14:47:00 -0500 7, 33 -- Cc: microscopy-at-microscopy.com 7, 33 -- Message-Id: {434A33A9-D5A4-4637-8E62-50313525D481-at-gmail.com} 7, 33 -- References: {201109131931.p8DJVd8v013372-at-ns.microscopy.com} 7, 33 -- To: r.sims-at-auckland.ac.nz 7, 33 -- X-Mailer: Apple Mail (2.1244.3) 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8DJl6V9022484 ==============================End of - Headers==============================
A colleague from a neighboring institution needs to get rid of a Philips CM10 TEM. He asked me if there are any companies that dealt in selling used TEMs, not a topic that I am current on. So, if anyone knows of any companies like that please let me know. I'm able to advise them on what it will take to dispose of it as far as hazmat considerations and the scrapping of the metals which can be done locally.
Tom Bargar University of Nebraska Medical Center Core Electron Microscopy Research Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
==============================Original Headers============================== 3, 21 -- From tbargar-at-unmc.edu Tue Sep 13 16:08:43 2011 3, 21 -- Received: from zixvpm02.unmc.edu (zixvpm02.unmc.edu [192.198.54.127]) 3, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8DL8hqs007386 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 16:08:43 -0500 3, 21 -- Received: from zixvpm02.unmc.edu (ZixVPM [127.0.0.1]) 3, 21 -- by Outbound.unmc.edu (Proprietary) with ESMTP id 27AD0C4014A 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 16:08:43 -0500 (CDT) 3, 21 -- Received: from unmcnotes01.unmc.edu (unknown [10.8.51.5]) 3, 21 -- by zixvpm02.unmc.edu (Proprietary) with ESMTP id E4B11C40132 3, 21 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Sep 2011 16:08:41 -0500 (CDT) 3, 21 -- To: Microscopy-at-microscopy.com 3, 21 -- MIME-Version: 1.0 3, 21 -- Subject: Need advice on disposing of a Philips CM10 3, 21 -- X-Mailer: Lotus Notes Release 8.0.2 August 07, 2008 3, 21 -- Message-ID: {OF0994FB11.52C0EC2E-ON8625790A.0073416F-8625790A.007426E7-at-unmc.edu} 3, 21 -- From: Tom W Bargar {tbargar-at-unmc.edu} 3, 21 -- Date: Tue, 13 Sep 2011 16:08:41 -0500 3, 21 -- X-MIMETrack: Serialize by Router on UNMCNOTES01.UNMC.EDU/Servers/UNEBR at 09/13/2011 04:08:42 3, 21 -- PM, 3, 21 -- Serialize complete at 09/13/2011 04:08:42 PM 3, 21 -- Content-Type: text/plain; charset="US-ASCII" ==============================End of - Headers==============================
Has anyone tried EBSD of high-Ni, high-Co Fe-based austenitic alloys? Our SEM only has one free time block between now and the project's end at end of fiscal year Sep 30, so I want to maximize my chances of getting good patterns for our one shot.
Can anyone recommend a final polish recipe? I'm leaning towards a multi-hour colloidal silica finish, but if anyone had good advice I would appreciate it.
Thanks, Chad
--------------------- Chad M. Parish, Ph.D. Microscopy Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov Web: www.ornl.gov/share
==============================Original Headers============================== 7, 34 -- From parishcm-at-ornl.gov Wed Sep 14 06:10:57 2011 7, 34 -- Received: from emroute4.ornl.gov (emroute4.ornl.gov [160.91.86.27]) 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8EBAvnE001501 7, 34 -- for {Microscopy-at-Microscopy.Com} ; Wed, 14 Sep 2011 06:10:57 -0500 7, 34 -- Received: from emroute4.ornl.gov ([127.0.0.1]) 7, 34 -- by emroute4.ornl.gov (PMDF V6.5-x6 #31890) 7, 34 -- with ESMTP id {0LRI00MQKF29JH-at-emroute4.ornl.gov} for 7, 34 -- Microscopy-at-Microscopy.Com; Wed, 14 Sep 2011 07:10:57 -0400 (EDT) 7, 34 -- Received: from CONVERSION-DAEMON.emroute4.ornl.gov by emroute4.ornl.gov 7, 34 -- (PMDF V6.5-x6 #31890) id {0LRI00401F292X-at-emroute4.ornl.gov} for 7, 34 -- Microscopy-at-Microscopy.Com; Wed, 14 Sep 2011 07:10:57 -0400 (EDT) 7, 34 -- Received: from exchhub1.ornl.gov (exchhub1.ornl.gov [160.91.2.111]) 7, 34 -- by emroute4.ornl.gov (PMDF V6.5-x6 #31890) 7, 34 -- with ESMTPS id {0LRI0030JF29Y5-at-emroute4.ornl.gov} for 7, 34 -- Microscopy-at-Microscopy.Com; Wed, 14 Sep 2011 07:10:57 -0400 (EDT) 7, 34 -- Received: from EXCHMB.ornl.gov ([160.91.2.201]) 7, 34 -- by exchhub1.ornl.gov ([160.91.2.111]) with mapi; Wed, 7, 34 -- 14 Sep 2011 07:10:57 -0400 7, 34 -- Date: Wed, 14 Sep 2011 07:10:55 -0400 7, 34 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 7, 34 -- Subject: EBSD: High-alloy austenitic steel sample prep 7, 34 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 7, 34 -- Message-id: {9D5E22CB860AB747B59E10EEB624BACC1BC1B2BC52-at-EXCHMB.ornl.gov} 7, 34 -- MIME-version: 1.0 7, 34 -- Content-type: text/plain; charset=us-ascii 7, 34 -- Content-language: en-US 7, 34 -- Thread-Topic: EBSD: High-alloy austenitic steel sample prep 7, 34 -- Thread-Index: AcxyzvlhnxakhqxARbavpNzHowzNpQ== 7, 34 -- Accept-Language: en-US 7, 34 -- acceptlanguage: en-US 7, 34 -- X-MS-Has-Attach: 7, 34 -- X-MS-TNEF-Correlator: 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8EBAvnE001501 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nano-at-nanogune.eu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nano-at-nanogune.eu Name: CIC nanogune
Organization: CIC nanogune
Title-Subject: [Filtered] Laboratory Technician
Message: CIC nanoGUNE Consolider, located in San Sebastian, Basque Country (Spain), is a R&D center created recently with the mission of conducting basic and applied world-class research in nanoscience and nanotechnology, fostering training and education excellence, and supporting the growth of a nanotechnology-based industry. A “Materials Synthesis” research group with strong focus on vacuum-based thin-film deposition methods is currently being established to accompany nanoGUNE´s already ongoing research activities. In order to provide qualified assistance to the research activities, we have an immediate opening for a technician/engineer. We are searching for a highly motivated and creative person with a solid technical/engineering background, mechanical and electronic design and construction skills, and experience in operating and maintaining thin-film processing equipment in terms of hardware and control software.
Requirements for this position are: − suitable technical or technical-engineering education − 5-years hands-on experience in construction and maintenance of technical equipment with focus on vacuum technology − basic experience control software programming (e.g., LabView and/or Siemens SPS) − good English communication skills
Responsibilities will include: − general instrument maintenance − development and construction of deposition instrumentation and control units − equipment and consumables purchase − engineering assistance of ongoing research and projects
Interested individuals should send a letter of application and their curriculum vitae under Ref: ALD technician application to nano-at-nanogune.eu
EBSD requires more than a mirror like finish. An ultra fine polish with silica is needed. I've sent samples out with a 1um diamond mirror finish and got terrible results. just my 2cents
-----Original Message----- X-from: larry.stoter-at-gmail.com [mailto:larry.stoter-at-gmail.com] Sent: Thursday, September 15, 2011 2:34 AM To: Frank Karl
Finish with an electro-chemical polish?
Dilute HCl (10%?) and ~15 V
I've never used for EBSD but have used for preparing TEM & SEM samples. Mirror-like defect free finish.
Larry Stoter (Working on a Microsoft-free computer)
On 14 Sep 2011, at 12:21, parishcm-at-ornl.gov wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Greetings, } } Has anyone tried EBSD of high-Ni, high-Co Fe-based austenitic alloys? Our SEM only has one free time block between now and the project's end at end of fiscal year Sep 30, so I want to maximize my chances of getting good patterns for our one shot. } } Can anyone recommend a final polish recipe? I'm leaning towards a multi-hour colloidal silica finish, but if anyone had good advice I would appreciate it. } } Thanks, } Chad } } --------------------- } Chad M. Parish, Ph.D. } Microscopy Group } Materials Science and Technology Division } Oak Ridge National Laboratory } Phone: 1 865 574 0092 } Email: parishcm-at-ornl.gov } Web: www.ornl.gov/share } } } } ==============================Original Headers============================== } 7, 34 -- From parishcm-at-ornl.gov Wed Sep 14 06:10:57 2011 } 7, 34 -- Received: from emroute4.ornl.gov (emroute4.ornl.gov [160.91.86.27]) } 7, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8EBAvnE001501 } 7, 34 -- for {Microscopy-at-Microscopy.Com} ; Wed, 14 Sep 2011 06:10:57 -0500 } 7, 34 -- Received: from emroute4.ornl.gov ([127.0.0.1]) } 7, 34 -- by emroute4.ornl.gov (PMDF V6.5-x6 #31890) } 7, 34 -- with ESMTP id {0LRI00MQKF29JH-at-emroute4.ornl.gov} for } 7, 34 -- Microscopy-at-Microscopy.Com; Wed, 14 Sep 2011 07:10:57 -0400 (EDT) } 7, 34 -- Received: from CONVERSION-DAEMON.emroute4.ornl.gov by emroute4.ornl.gov } 7, 34 -- (PMDF V6.5-x6 #31890) id {0LRI00401F292X-at-emroute4.ornl.gov} for } 7, 34 -- Microscopy-at-Microscopy.Com; Wed, 14 Sep 2011 07:10:57 -0400 (EDT) } 7, 34 -- Received: from exchhub1.ornl.gov (exchhub1.ornl.gov [160.91.2.111]) } 7, 34 -- by emroute4.ornl.gov (PMDF V6.5-x6 #31890) } 7, 34 -- with ESMTPS id {0LRI0030JF29Y5-at-emroute4.ornl.gov} for } 7, 34 -- Microscopy-at-Microscopy.Com; Wed, 14 Sep 2011 07:10:57 -0400 (EDT) } 7, 34 -- Received: from EXCHMB.ornl.gov ([160.91.2.201]) } 7, 34 -- by exchhub1.ornl.gov ([160.91.2.111]) with mapi; Wed, } 7, 34 -- 14 Sep 2011 07:10:57 -0400 } 7, 34 -- Date: Wed, 14 Sep 2011 07:10:55 -0400 } 7, 34 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} } 7, 34 -- Subject: EBSD: High-alloy austenitic steel sample prep } 7, 34 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} } 7, 34 -- Message-id: {9D5E22CB860AB747B59E10EEB624BACC1BC1B2BC52-at-EXCHMB.ornl.gov} } 7, 34 -- MIME-version: 1.0 } 7, 34 -- Content-type: text/plain; charset=us-ascii } 7, 34 -- Content-language: en-US } 7, 34 -- Thread-Topic: EBSD: High-alloy austenitic steel sample prep } 7, 34 -- Thread-Index: AcxyzvlhnxakhqxARbavpNzHowzNpQ== } 7, 34 -- Accept-Language: en-US } 7, 34 -- acceptlanguage: en-US } 7, 34 -- X-MS-Has-Attach: } 7, 34 -- X-MS-TNEF-Correlator: } 7, 34 -- Content-Transfer-Encoding: 8bit } 7, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8EBAvnE001501 } ==============================End of - Headers==============================
==============================Original Headers============================== 8, 32 -- From larry.stoter-at-gmail.com Thu Sep 15 01:20:19 2011 8, 32 -- Received: from mail-wy0-f169.google.com (mail-wy0-f169.google.com [74.125.82.169]) 8, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8F6KIm8005904 8, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Sep 2011 01:20:18 -0500 8, 32 -- Received: by wyi11 with SMTP id 11so2520556wyi.0 8, 32 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Sep 2011 23:20:18 -0700 (PDT) 8, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 32 -- d=gmail.com; s=gamma; 8, 32 -- h=content-type:mime-version:subject:from:in-reply-to:date 8, 32 -- :content-transfer-encoding:message-id:references:to:x-mailer; 8, 32 -- bh=fNCKt/oujxTaTEEFk6SJinfGlpJF/LegVs8HfMJvIR4=; 8, 32 -- b=D6TgDvtXe8H9fAHuj7mpK69FcMDu/6Xc96li7qXoD7o8PzECeuzP45tv3B65UgRyyU 8, 32 -- 8plygKEh5o9SIBTqaSJE+He9apuAGDeot0DGr2qIAtaMK+iaaoIDYIDNmLllyTWuaAai 8, 32 -- IbHQPJQWVOI4xTKpZQskk/EXbxuOW1L5gDj4M= 8, 32 -- Received: by 10.227.208.65 with SMTP id gb1mr720772wbb.32.1316067617827; 8, 32 -- Wed, 14 Sep 2011 23:20:17 -0700 (PDT) 8, 32 -- Received: from [10.175.1.100] ([87.115.86.207]) 8, 32 -- by mx.google.com with ESMTPS id i11sm6748225wbn.25.2011.09.14.23.20.15 8, 32 -- (version=TLSv1/SSLv3 cipher=OTHER); 8, 32 -- Wed, 14 Sep 2011 23:20:16 -0700 (PDT) 8, 32 -- Content-Type: text/plain; charset=us-ascii 8, 32 -- Mime-Version: 1.0 (Apple Message framework v1244.3) 8, 32 -- Subject: Re: [Microscopy] EBSD: High-alloy austenitic steel sample prep 8, 32 -- From: Larry Stoter {larry.stoter-at-gmail.com} 8, 32 -- In-Reply-To: {201109141121.p8EBLnl9017872-at-ns.microscopy.com} 8, 32 -- Date: Thu, 15 Sep 2011 07:20:14 +0100 8, 32 -- Message-Id: {F4F17B2C-789D-4E48-B3BD-D7B23DFC482E-at-gmail.com} 8, 32 -- References: {201109141121.p8EBLnl9017872-at-ns.microscopy.com} 8, 32 -- To: parishcm-at-ornl.gov, MSA Microscopy List {Microscopy-at-microscopy.com} 8, 32 -- X-Mailer: Apple Mail (2.1244.3) 8, 32 -- Content-Transfer-Encoding: 8bit 8, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8F6KIm8005904 ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nesmicroscopy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: nesmicroscopy-at-gmail.com Name: NESM
Organization: New England Society for Microscopy
Title-Subject: [Filtered] SAVE-THE-DATE: NESM Fall Meeting - October 20th
Message: NESM FALL MEETING - October 20, 2011
Hello Microscopy ListServerites,
Save-the-Date: October 20th For the upcoming New England Society for Microscopy's Fall Meeting -at- Center for Biological Imaging, Harvard University Cambridge
Thursday, October 20 -- Workshops (1:30PM-4:00PM) and Dinner Meeting (4:00PM-9:00PM)
Registration information will be distributed in the coming week...
Tentative Meeting Schedule: 1:30-2:00 Workshop registration at the Center for Biological Imaging (CBI) 2:00-4:00 Workshops: X-ray MicroCT, CARS, and Super Resolution SI/PAL-M 4:00-5:00 Meeting registration and tour of CBI (including refreshments) 5:30-6:30 "The Human Connectome Project", Jeff Lichtman, Ph.D., Harvard University (http://www.humanconnectomeproject.org/) 6:30-7:30 Dinner 7:45-8:45 "Super Resolution Light Microscopy: Structured Illumination and PAL-M", Bernhard Goetze, Ph.D., Zeiss
Workshop Cost (workshops will run concurrently, so you may attend only one with space limited to 4 people per workshop) $15 All Members
Dinner Meeting Costs (including refreshments, dinner and two talks starting at 5:30PM): $20 NESM Members $45 Nonmembers (includes 2012-year membership) $10 Students $10 Retirees Walk-ins: Additional $5
Bring A Colleague: NESM members who bring two new members to join during 2012, will receive free membership for 2013!!!
Thank you for your attention and consideration; and we look forward to seeing you in October.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both gunter.moeller-at-arkema.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: gunter.moeller-at-arkema.com
Name: Gunter Moeller
Organization: ARKEMA Inc.
Title-Subject: [Filtered] SEM reliability experience, service contracts
Message: Guys,
We are planning to purchase a new SEM this year and many of you are probably much more experienced in SEM than I am.
Instruments we are considering are: - JEOL JSM-7001F (formerly 6701F) - JEOL JSM-7600F - Carl Zeiss SIGMA - Carl Zeiss SUPRA - Hitachi SU8000 series (SU8010) - FEI Inspect F50 FEG
I was wondering if you would like to share information regarding reliability and any other noteworthy experiences with these or similar instruments. We also would appreciate information regarding the timeliness and quality of service provided. We will purchase a service contract. We are located in PA. We do high and low mag work with EDS and most of our samples are polymers. You can reach me also at: 610-878-6384 or at gunter.moeller-at-arkema.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ressl006-at-umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ressl006-at-umn.edu
Name: Alice Ressler
Organization: University of Minnesota Characterization Facility
Title-Subject: [Filtered] calculating LN2 cost Message: When ordering or pricing supplies how does your lab factor in the costs for acquiring, obtaining and moving tanks of LN2 when calculating a per liter cost to users?
Hello List servers, We have a problem with 0.5 um semi-thin nerve sections processed for standard EM (immersion fixed, reduced osmium). Basically the center of the axon bundle stains lighter than the rest of the tissue with methylene blue azure 2 borate. Is this a fixation or infiltration (Parts epon to propylene oxide) problem? Seems like they section well but I am guessing uneven cutting where the centers are lighter. Any thoughts??
Michael Delannoy.
==============================Original Headers============================== 3, 30 -- From prvs=233d3a685=delannoy-at-jhmi.edu Fri Sep 16 08:04:16 2011 3, 30 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GD4G9I008954 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 08:04:16 -0500 3, 30 -- X-IronPort-AV: E=Sophos;i="4.68,393,1312171200"; 3, 30 -- d="scan'208";a="142748830" 3, 30 -- Received: from jhem01.johnshopkins.edu (HELO commsmail.johnshopkins.edu) ([10.173.7.11]) 3, 30 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 16 Sep 2011 09:04:15 -0400 3, 30 -- MIME-version: 1.0 3, 30 -- Content-transfer-encoding: 7BIT 3, 30 -- Content-disposition: inline 3, 30 -- Content-type: text/plain; CHARSET=US-ASCII 3, 30 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 3, 30 -- by commsmail.johnshopkins.edu 3, 30 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 3, 30 -- with ESMTP id {0LRM003X19N3LL30-at-commsmail.johnshopkins.edu} for 3, 30 -- microscopy-at-microscopy.com; Fri, 16 Sep 2011 09:04:15 -0400 (EDT) 3, 30 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.62]) 3, 30 -- by commsmail.johnshopkins.edu (mshttpd); Fri, 16 Sep 2011 09:04:15 -0400 3, 30 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 3, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 3, 30 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 3, 30 -- Message-id: {72a0d9d810c93.4e73110f-at-johnshopkins.edu} 3, 30 -- Date: Fri, 16 Sep 2011 09:04:15 -0400 3, 30 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 3, 30 -- 2009) 3, 30 -- Content-language: en 3, 30 -- Subject: semi-thin nerve sections uneven staining 3, 30 -- X-Accept-Language: en 3, 30 -- Priority: normal ==============================End of - Headers==============================
Michael - I am not sure I buy the uneven cutting hypothesis but it is easy to check. Trim the edges off a block and see if the center now stains differently. Or cut the section at 1.0 um thickness and see if the middle region looks better. My experience with other types of tissue is that regions of semi-thin sections that stain poorly with toluidine blue are poorly fixed. In a few cases it has resulted from bad polymerization. If the problem is really fixation or embedding, I don't think cutting thicker sections will help as much as one would predict based on well-fixed and embedded tissues. Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: delannoy-at-jhmi.edu [mailto:delannoy-at-jhmi.edu] Sent: Friday, September 16, 2011 8:05 AM To: Phillips, Thomas E.
Hello List servers, We have a problem with 0.5 um semi-thin nerve sections processed for standard EM (immersion fixed, reduced osmium). Basically the center of the axon bundle stains lighter than the rest of the tissue with methylene blue azure 2 borate. Is this a fixation or infiltration (Parts epon to propylene oxide) problem? Seems like they section well but I am guessing uneven cutting where the centers are lighter. Any thoughts??
Michael Delannoy.
==============================Original Headers============================== 3, 30 -- From prvs=233d3a685=delannoy-at-jhmi.edu Fri Sep 16 08:04:16 2011 3, 30 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GD4G9I008954 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 08:04:16 -0500 3, 30 -- X-IronPort-AV: E=Sophos;i="4.68,393,1312171200"; 3, 30 -- d="scan'208";a="142748830" 3, 30 -- Received: from jhem01.johnshopkins.edu (HELO commsmail.johnshopkins.edu) ([10.173.7.11]) 3, 30 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 16 Sep 2011 09:04:15 -0400 3, 30 -- MIME-version: 1.0 3, 30 -- Content-transfer-encoding: 7BIT 3, 30 -- Content-disposition: inline 3, 30 -- Content-type: text/plain; CHARSET=US-ASCII 3, 30 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 3, 30 -- by commsmail.johnshopkins.edu 3, 30 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 3, 30 -- with ESMTP id {0LRM003X19N3LL30-at-commsmail.johnshopkins.edu} for 3, 30 -- microscopy-at-microscopy.com; Fri, 16 Sep 2011 09:04:15 -0400 (EDT) 3, 30 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.62]) 3, 30 -- by commsmail.johnshopkins.edu (mshttpd); Fri, 16 Sep 2011 09:04:15 -0400 3, 30 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 3, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 3, 30 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 3, 30 -- Message-id: {72a0d9d810c93.4e73110f-at-johnshopkins.edu} 3, 30 -- Date: Fri, 16 Sep 2011 09:04:15 -0400 3, 30 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 3, 30 -- 2009) 3, 30 -- Content-language: en 3, 30 -- Subject: semi-thin nerve sections uneven staining 3, 30 -- X-Accept-Language: en 3, 30 -- Priority: normal ==============================End of - Headers==============================
Hi Michael, I agree with Thomas' suggestion that the uneven methyline blue staining is due to poor fixation. In my experience, it is due to incomplete osmium penetration throughout the tissue. Frank
Frank Macaluso Administrative Director and Director of Electron Microscopy Analytical Imaging Facility Albert Einstein College of Medicine 1300 Morris Park Avenue Bronx, NY 10461
________________________________________ X-from: delannoy-at-jhmi.edu [delannoy-at-jhmi.edu] Sent: Friday, September 16, 2011 9:17 AM To: Frank P Macaluso
Hello List servers, We have a problem with 0.5 um semi-thin nerve sections processed for standard EM (immersion fixed, reduced osmium). Basically the center of the axon bundle stains lighter than the rest of the tissue with methylene blue azure 2 borate. Is this a fixation or infiltration (Parts epon to propylene oxide) problem? Seems like they section well but I am guessing uneven cutting where the centers are lighter. Any thoughts??
Michael Delannoy.
==============================Original Headers============================== 3, 30 -- From prvs=233d3a685=delannoy-at-jhmi.edu Fri Sep 16 08:04:16 2011 3, 30 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GD4G9I008954 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 08:04:16 -0500 3, 30 -- X-IronPort-AV: E=Sophos;i="4.68,393,1312171200"; 3, 30 -- d="scan'208";a="142748830" 3, 30 -- Received: from jhem01.johnshopkins.edu (HELO commsmail.johnshopkins.edu) ([10.173.7.11]) 3, 30 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 16 Sep 2011 09:04:15 -0400 3, 30 -- MIME-version: 1.0 3, 30 -- Content-transfer-encoding: 7BIT 3, 30 -- Content-disposition: inline 3, 30 -- Content-type: text/plain; CHARSET=US-ASCII 3, 30 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 3, 30 -- by commsmail.johnshopkins.edu 3, 30 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 3, 30 -- with ESMTP id {0LRM003X19N3LL30-at-commsmail.johnshopkins.edu} for 3, 30 -- microscopy-at-microscopy.com; Fri, 16 Sep 2011 09:04:15 -0400 (EDT) 3, 30 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.62]) 3, 30 -- by commsmail.johnshopkins.edu (mshttpd); Fri, 16 Sep 2011 09:04:15 -0400 3, 30 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 3, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 3, 30 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} 3, 30 -- Message-id: {72a0d9d810c93.4e73110f-at-johnshopkins.edu} 3, 30 -- Date: Fri, 16 Sep 2011 09:04:15 -0400 3, 30 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 3, 30 -- 2009) 3, 30 -- Content-language: en 3, 30 -- Subject: semi-thin nerve sections uneven staining 3, 30 -- X-Accept-Language: en 3, 30 -- Priority: normal ==============================End of - Headers==============================
==============================Original Headers============================== 13, 43 -- From frank.macaluso-at-einstein.yu.edu Fri Sep 16 09:44:32 2011 13, 43 -- Received: from mx2.mc.yu.edu (mx2.mc.yu.edu [129.98.201.102]) 13, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GEiWXi011243 13, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 09:44:32 -0500 13, 43 -- Received: from phobos.mc.yu.edu (phobos.mc.yu.edu [129.98.201.101]) 13, 43 -- by mx2.mc.yu.edu (Postfix) with ESMTP id 2D2CF9C838F 13, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 10:44:32 -0400 (EDT) 13, 43 -- X-AuditID: 8162c965-a249abb0000059f1-c5-4e735964a3f3 13, 43 -- Received: from fe6.prod.mis.yu.edu (deliver.mc.yu.edu [129.98.201.63]) 13, 43 -- by phobos.mc.yu.edu (Symantec Mail Security) with ESMTP id 35F79328003 13, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 10:12:52 -0400 (EDT) 13, 43 -- X-Spam-Checker-Version: SpamAssassin 3.2.5 (2008-06-10) on fe6.prod.mis.yu.edu 13, 43 -- X-Spam-Level: 13, 43 -- X-Spam-Status: No, score=0.3 required=5.0 tests=AWL,RDNS_NONE 13, 43 -- autolearn=disabled version=3.2.5 13, 43 -- Received: from smtp.yu.edu (unknown [129.98.255.141]) 13, 43 -- (using TLSv1 with cipher AES128-SHA (128/128 bits)) 13, 43 -- (No client certificate requested) 13, 43 -- by fe6.prod.mis.yu.edu (Postfix) with ESMTPS id 827CA29C59; 13, 43 -- Fri, 16 Sep 2011 10:44:28 -0400 (EDT) 13, 43 -- Received: from YUWEXCPM12.yuad.uds.yu.edu ([fe80::e881:9525:79eb:6200]) by 13, 43 -- YUWEXCPC02.yuad.uds.yu.edu ([129.98.255.141]) with mapi id 14.01.0339.001; 13, 43 -- Fri, 16 Sep 2011 10:44:28 -0400 13, 43 -- From: Frank P Macaluso {frank.macaluso-at-einstein.yu.edu} 13, 43 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 43 -- CC: "delannoy-at-jhmi.edu" {delannoy-at-jhmi.edu} 13, 43 -- Subject: FW: [Microscopy] semi-thin nerve sections uneven staining 13, 43 -- Thread-Topic: [Microscopy] semi-thin nerve sections uneven staining 13, 43 -- Thread-Index: AQHMdHL3ZGt8sXOevEq0RFumuAb0PJVQE+tR 13, 43 -- Date: Fri, 16 Sep 2011 14:44:26 +0000 13, 43 -- Message-ID: {F307DABD0824D645BEBF79398868642A0213BFEA-at-YUWEXCPM12.yuad.uds.yu.edu} 13, 43 -- References: {201109161317.p8GDHJ75026661-at-ns.microscopy.com} 13, 43 -- In-Reply-To: {201109161317.p8GDHJ75026661-at-ns.microscopy.com} 13, 43 -- Accept-Language: en-US 13, 43 -- Content-Language: en-US 13, 43 -- X-MS-Has-Attach: 13, 43 -- X-MS-TNEF-Correlator: 13, 43 -- x-originating-ip: [129.98.31.156] 13, 43 -- Content-Type: text/plain; charset="us-ascii" 13, 43 -- MIME-Version: 1.0 13, 43 -- X-Brightmail-Tracker: AAAAAA== 13, 43 -- Content-Transfer-Encoding: 8bit 13, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8GEiWXi011243 ==============================End of - Headers==============================
Dear Group - I have a camera lucida attached to the vertical tube on a trinocular compound microscope. Â I need an ocular lens that is 41 mm in length and 24mm wide. Any suggestions would be great. Thank you. Barbara
==============================Original Headers============================== 2, 26 -- From maloneyb10-at-gmail.com Fri Sep 16 10:55:22 2011 2, 26 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GFtLDo029013 2, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 10:55:21 -0500 2, 26 -- Received: by pzk4 with SMTP id 4so2300253pzk.0 2, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 08:55:21 -0700 (PDT) 2, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 26 -- d=gmail.com; s=gamma; 2, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 2, 26 -- :content-transfer-encoding; 2, 26 -- bh=s1/WnrxBKMSSD3ElMXzAo+0fK3hrOl3dami0thBaz68=; 2, 26 -- b=RVPcKtAo3zDKXgPqF+9QnQz4U6MpozBDClZBaVx5lvAraNJYgKRz7WETj83wESiGqy 2, 26 -- TquZn2gz+sRUFlsfP+7S/33Y6GYbrvR9yVjzPDrl+stOd+1nW4pw96rhPWfL6PR4Y3wz 2, 26 -- eDgIMugOAu5mOXxf2lDH03vQx2n7exenB+O7o= 2, 26 -- MIME-Version: 1.0 2, 26 -- Received: by 10.68.49.10 with SMTP id q10mr464560pbn.341.1316188521300; Fri, 2, 26 -- 16 Sep 2011 08:55:21 -0700 (PDT) 2, 26 -- Received: by 10.68.43.133 with HTTP; Fri, 16 Sep 2011 08:55:21 -0700 (PDT) 2, 26 -- Date: Fri, 16 Sep 2011 11:55:21 -0400 2, 26 -- Message-ID: {CAJpMqP=z6KAdYmpxLZ7covuvwhYNOFFEHgex4g7jZfnCmOTQ6Q-at-mail.gmail.com} 2, 26 -- Subject: LM -need short ocular lens 2, 26 -- From: B Maloney {maloneyb10-at-gmail.com} 2, 26 -- To: Microscopy-at-microscopy.com 2, 26 -- Content-Type: text/plain; charset=UTF-8 2, 26 -- Content-Transfer-Encoding: 8bit 2, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8GFtLDo029013 ==============================End of - Headers==============================
Greetings Michael. We have seen similar problems in semi-thin cross sections of oriented mouse nerve and rat spinal cord (too big to be allowed!). We have achieved consistent success by extending our standard secondary fixation of 1 hour at room temperature on a rotator with 1% buffered osmium tetroxide. Instead we rotate at room temperature for 2 hours, exchange with fresh osmium solution and leave the nerve samples in osmium in the refrigerator for 24 hours. Samples are then brought to room temperature, given 3 buffer rinses and processed per normal protocol.
The sooner the secondary fixation follows the primary fixation, the better, but we have gotten good results from samples that have been in fix for a week or more. Good luck!
Yours, Matt
Matthew Stephenson Cell Imaging Shared Resource (CISR) Vanderbilt University Medical Center (615) 322-5965; 343-6691 m.stephenson-at-vanderbilt.edu
==============================Original Headers============================== 3, 43 -- From m.stephenson-at-Vanderbilt.Edu Fri Sep 16 13:20:57 2011 3, 43 -- Received: from DB3EHSOBE003.bigfish.com (db3ehsobe003.messaging.microsoft.com [213.199.154.141]) 3, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GIKvBZ015661 3, 43 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 13:20:57 -0500 3, 43 -- Received: from mail84-db3-R.bigfish.com (10.3.81.240) by 3, 43 -- DB3EHSOBE003.bigfish.com (10.3.84.23) with Microsoft SMTP Server id 3, 43 -- 14.1.225.22; Fri, 16 Sep 2011 18:20:56 +0000 3, 43 -- Received: from mail84-db3 (localhost.localdomain [127.0.0.1]) by 3, 43 -- mail84-db3-R.bigfish.com (Postfix) with ESMTP id C3D025E82E3 for 3, 43 -- {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 18:20:56 +0000 (UTC) 3, 43 -- X-SpamScore: 0 3, 43 -- X-BigFish: VPS0(zzzz1202hzzz2dh2a8h668h839h944h) 3, 43 -- X-Forefront-Antispam-Report: CIP:129.59.94.38;KIP:(null);UIP:(null);IPVD:NLI;H:hub.vanderbilt.edu;RD:none;EFVD:NLI 3, 43 -- X-FB-SS: 13, 3, 43 -- Received: from mail84-db3 (localhost.localdomain [127.0.0.1]) by mail84-db3 3, 43 -- (MessageSwitch) id 1316197224883125_22612; Fri, 16 Sep 2011 18:20:24 +0000 3, 43 -- (UTC) 3, 43 -- Received: from DB3EHSMHS017.bigfish.com (unknown [10.3.81.242]) by 3, 43 -- mail84-db3.bigfish.com (Postfix) with ESMTP id 383C4167012C for 3, 43 -- {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 18:19:36 +0000 (UTC) 3, 43 -- Received: from hub.vanderbilt.edu (129.59.94.38) by DB3EHSMHS017.bigfish.com 3, 43 -- (10.3.87.117) with Microsoft SMTP Server (TLS) id 14.1.225.22; Fri, 16 Sep 3, 43 -- 2011 18:19:36 +0000 3, 43 -- Received: from ITS-HCWNEM06.ds.Vanderbilt.edu ([10.1.137.106]) by 3, 43 -- ITS-SCWNEM26.ds.Vanderbilt.edu ([fe80::b8bb:fb81:d5ba:1d6%11]) with mapi; 3, 43 -- Fri, 16 Sep 2011 13:19:30 -0500 3, 43 -- From: "Stephenson, Matthew K" {m.stephenson-at-Vanderbilt.Edu} 3, 43 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 3, 43 -- Date: Fri, 16 Sep 2011 13:19:30 -0500 3, 43 -- Subject: Re: semi-thin nerve sections uneven staining 3, 43 -- Thread-Topic: semi-thin nerve sections uneven staining 3, 43 -- Thread-Index: AQHMdJ0tEoLXtrmHT024UCBKhVIvSA== 3, 43 -- Message-ID: {B012EC67F417F14CA28FFC6CC92807BC165879916D-at-ITS-HCWNEM06.ds.Vanderbilt.edu} 3, 43 -- Accept-Language: en-US 3, 43 -- Content-Language: en-US 3, 43 -- X-MS-Has-Attach: 3, 43 -- X-MS-TNEF-Correlator: 3, 43 -- acceptlanguage: en-US 3, 43 -- Content-Type: text/plain; charset="us-ascii" 3, 43 -- MIME-Version: 1.0 3, 43 -- X-OriginatorOrg: vanderbilt.edu 3, 43 -- Content-Transfer-Encoding: 8bit 3, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8GIKvBZ015661 ==============================End of - Headers==============================
Dear Group: I apologize, should have given additional information. I have a Westover trinocular compound microscope and a camera lucida that did not come with the scope. Any ocular seems to fit in the vertical tube, however, when folding the camera lucida over the ocular lens, it doesn't sit flat and need a shorter ocular lens. Any suggestions would be greatly appreciated. Thanks Barbara
==============================Original Headers============================== 1, 23 -- From maloneyb10-at-gmail.com Fri Sep 16 14:46:53 2011 1, 23 -- Received: from mail-yi0-f41.google.com (mail-yi0-f41.google.com [209.85.218.41]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GJkrR3001313 1, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 14:46:53 -0500 1, 23 -- Received: by yia25 with SMTP id 25so3054309yia.0 1, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 12:46:52 -0700 (PDT) 1, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 23 -- d=gmail.com; s=gamma; 1, 23 -- h=mime-version:date:message-id:subject:from:to:content-type; 1, 23 -- bh=LrKt0U1EJiu1N6UCbaaTPWvFQk/Irk2mfRfeFT1ECUo=; 1, 23 -- b=MzA6COEEW5h69mXww3SsNRxpAonXzjvtAPrs9ymiHk87+IVtRYR4nfkk21bghk/yj5 1, 23 -- zZrazeB3pVJVHaTFudgkm5QY5f71BW2PI4jNvzr47o41Oh8C3nEjBleJHKnbU0C2jA6F 1, 23 -- lkkqdeZAhv7fXHBACruB3EESfyuurY1kvih2E= 1, 23 -- MIME-Version: 1.0 1, 23 -- Received: by 10.68.38.135 with SMTP id g7mr601091pbk.140.1316202412303; Fri, 1, 23 -- 16 Sep 2011 12:46:52 -0700 (PDT) 1, 23 -- Received: by 10.68.43.133 with HTTP; Fri, 16 Sep 2011 12:46:52 -0700 (PDT) 1, 23 -- Date: Fri, 16 Sep 2011 15:46:52 -0400 1, 23 -- Message-ID: {CAJpMqP=w1WGkafP0k+c74YUJ_L274nLLHja4ivfC4Hv_2sbzhQ-at-mail.gmail.com} 1, 23 -- Subject: Need a short ocular lens for Camera lucida 1, 23 -- From: B Maloney {maloneyb10-at-gmail.com} 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both scopes.master-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: scopes.master-at-gmail.com Name: Hamid R. Shakeri
Organization: Aviceena metallurgical Research Services
Title-Subject: [Filtered] Looking for a veteran electron microscopist
Message: Hi, looking for a gentlemen named Jan Englich (not sure of spelling). He used to work at Advanced Biotech, 9108 Guilford Rd., Columbia MD 21045. I need to get in touch with him with regard to an equipment he used to work with. If you know the gentleman please pass my email to him that is: scopes.master-at-gmail.com Please ask him to contact me.
Dear Listers, at the beginning of August I posted about a problem of the filament heating current in our SEM Jeol JSM-5600LV. It has turned out that problem is due to a damage in the High Voltage cable. So I'm wondering if anyone of you has such a cable which is not used and which could be sold to us, e.g. from a dismissed instrument. Quite improbable, I know, but never say never :) Anyone with something potentially useful for our instrument, please mail me. Thanks
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 6, 19 -- From dcristofori-at-unive.it Mon Sep 19 08:42:16 2011 6, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8JDgFbl006836 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 08:42:16 -0500 6, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 6, 19 -- (authenticated bits=0) 6, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p8JDg9e3010016 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 15:42:14 +0200 6, 19 -- Message-ID: {4E7749BB.7040100-at-unive.it} 6, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 6, 19 -- Date: Mon, 19 Sep 2011 15:55:07 +0200 6, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 6, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0.2) Gecko/20110902 Thunderbird/6.0.2 6, 19 -- MIME-Version: 1.0 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- Subject: SEM - filament heating current (reprised) 6, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I agree with Thomas and Frank, poor fixation in the center of the specimen and/or poor penetration by osmium or less for the osmium to stain.
Geoff
On 9/16/2011 9:05 AM, delannoy-at-jhmi.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello List servers, } We have a problem with 0.5 um semi-thin nerve sections processed for standard EM (immersion fixed, reduced osmium). Basically the center of the axon bundle stains lighter than the rest of the tissue with methylene blue azure 2 borate. Is this a fixation or infiltration (Parts epon to propylene oxide) problem? Seems like they section well but } I am guessing uneven cutting where the centers are lighter. Any thoughts?? } } Michael Delannoy. } } } ==============================Original Headers============================== } 3, 30 -- From prvs=233d3a685=delannoy-at-jhmi.edu Fri Sep 16 08:04:16 2011 } 3, 30 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) } 3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8GD4G9I008954 } 3, 30 -- for {microscopy-at-microscopy.com} ; Fri, 16 Sep 2011 08:04:16 -0500 } 3, 30 -- X-IronPort-AV: E=Sophos;i="4.68,393,1312171200"; } 3, 30 -- d="scan'208";a="142748830" } 3, 30 -- Received: from jhem01.johnshopkins.edu (HELO commsmail.johnshopkins.edu) ([10.173.7.11]) } 3, 30 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 16 Sep 2011 09:04:15 -0400 } 3, 30 -- MIME-version: 1.0 } 3, 30 -- Content-transfer-encoding: 7BIT } 3, 30 -- Content-disposition: inline } 3, 30 -- Content-type: text/plain; CHARSET=US-ASCII } 3, 30 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) } 3, 30 -- by commsmail.johnshopkins.edu } 3, 30 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) } 3, 30 -- with ESMTP id {0LRM003X19N3LL30-at-commsmail.johnshopkins.edu} for } 3, 30 -- microscopy-at-microscopy.com; Fri, 16 Sep 2011 09:04:15 -0400 (EDT) } 3, 30 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.62]) } 3, 30 -- by commsmail.johnshopkins.edu (mshttpd); Fri, 16 Sep 2011 09:04:15 -0400 } 3, 30 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} } 3, 30 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , } 3, 30 -- "microscopy-at-msa.microscopy.com" {microscopy-at-msa.microscopy.com} } 3, 30 -- Message-id: {72a0d9d810c93.4e73110f-at-johnshopkins.edu} } 3, 30 -- Date: Fri, 16 Sep 2011 09:04:15 -0400 } 3, 30 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 } 3, 30 -- 2009) } 3, 30 -- Content-language: en } 3, 30 -- Subject: semi-thin nerve sections uneven staining } 3, 30 -- X-Accept-Language: en } 3, 30 -- Priority: normal } ==============================End of - Headers============================== } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
May I suggest you take a look at organisations that deal with other types of high voltage systems, x-ray sets etc? I have found, working around the world, that organisations other than the instrument manufacturer are quite capable of renewing HV cables.
If you let them have your cable they will take the cable ends and fit them to a new cable and things will be as good as new.
Good luck
Steve
Steve Chapman FRMS Senior Consultant Protrain For consultancy and training in electron microscopy world wide Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967 www.emcourses.com
-----Original Message----- X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it] Sent: 19 September 2011 14:43 To: protrain-at-emcourses.com
Dear Listers, at the beginning of August I posted about a problem of the filament heating current in our SEM Jeol JSM-5600LV. It has turned out that problem is due to a damage in the High Voltage cable. So I'm wondering if anyone of you has such a cable which is not used and which could be sold to us, e.g. from a dismissed instrument. Quite improbable, I know, but never say never :) Anyone with something potentially useful for our instrument, please mail me. Thanks
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
==============================Original Headers============================== 6, 19 -- From dcristofori-at-unive.it Mon Sep 19 08:42:16 2011 6, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8JDgFbl006836 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 08:42:16 -0500 6, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) 6, 19 -- (authenticated bits=0) 6, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p8JDg9e3010016 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 15:42:14 +0200 6, 19 -- Message-ID: {4E7749BB.7040100-at-unive.it} 6, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} 6, 19 -- Date: Mon, 19 Sep 2011 15:55:07 +0200 6, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} 6, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0.2) Gecko/20110902 Thunderbird/6.0.2 6, 19 -- MIME-Version: 1.0 6, 19 -- To: Microscopy-at-microscopy.com 6, 19 -- Subject: SEM - filament heating current (reprised) 6, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Earlier this year we were forced to develop capability of custom-making high voltage cables for 50KV service and molding silicone connectors on them.
http://www.freudlabs.com/micrion_fib_spare_parts
I would welcome opportunity to apply this technology to HV cables for other FIB/SEM systems. Cost of making just one cable of the new type can get high because of the need to design and make molds for it, but in any case it is likely to remain far more affordable then purchase of the new SEM.
Fell free to get in touch, if you will want to explore this possibility.
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/19/2011 9:42 AM, dcristofori-at-unive.it wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } at the beginning of August I posted about a problem of the filament } heating current in our SEM Jeol JSM-5600LV. } It has turned out that problem is due to a damage in the High Voltage } cable. } So I'm wondering if anyone of you has such a cable which is not used and } which could be sold to us, e.g. from a dismissed instrument. Quite } improbable, I know, but never say never :) } Anyone with something potentially useful for our instrument, please mail me. } Thanks } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Universita' Ca' Foscari Venezia } Dipartimento di Sc. Molecolari e Nanosistemi } Lab. di Scienza e Tecnologia dei Materiali } Via Torino, 155b } I-30172 Mestre (VE) } Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } ==============================Original Headers============================== } 6, 19 -- From dcristofori-at-unive.it Mon Sep 19 08:42:16 2011 } 6, 19 -- Received: from algol.unive.it (algol.unive.it [157.138.1.8]) } 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8JDgFbl006836 } 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 08:42:16 -0500 } 6, 19 -- Received: from [127.0.0.1] (battagliarin.dcf.unive.it [157.138.23.17]) } 6, 19 -- (authenticated bits=0) } 6, 19 -- by algol.unive.it (8.13.8/8.13.8) with ESMTP id p8JDg9e3010016 } 6, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) } 6, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 15:42:14 +0200 } 6, 19 -- Message-ID: {4E7749BB.7040100-at-unive.it} } 6, 19 -- Disposition-Notification-To: Davide Cristofori {dcristofori-at-unive.it} } 6, 19 -- Date: Mon, 19 Sep 2011 15:55:07 +0200 } 6, 19 -- From: Davide Cristofori {dcristofori-at-unive.it} } 6, 19 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0.2) Gecko/20110902 Thunderbird/6.0.2 } 6, 19 -- MIME-Version: 1.0 } 6, 19 -- To: Microscopy-at-microscopy.com } 6, 19 -- Subject: SEM - filament heating current (reprised) } 6, 19 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed } 6, 19 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
The following position is now open for applications at the CAMCOR shared user facility at the University of Oregon.
http://camcor.uoregon.edu/
Please contact John Donovan for details.
mailto:donovan-at-uoregon.edu
The MicroAnalytical Research Assistant position provides professional technical and scientific assistance to support the CAMCOR MicroAnalytical laboratory and associated Inorganic Sample Preparation Laboratory under the direction and instruction of the MicroAnalytical Facility Director. As an entry level position, the MicroAnalytical Research Assistant will be expected to learn all necessary techniques and methods through self-instruction, tutoring, classes, professional development, scientific conferences, and other educational opportunities as available.
The MicroAnalytical Research Assistant will be expected to maintain the instrument laboratory and sample preparation areas, prepare samples of a geological, materials and archeological nature using a variety of sample preparation techniques. The MicroAnalytical Research Assistant will also operate optical and stereo microscopes and perform qualitative and quantitative analysis using a scanning electron microscope (FEI Quanta FEG SEM) and two electron probe micro analysis instruments (Cameca SX50 EPMA and SX100 EPMA).
The MicroAnalytical Research Assistant will also provide assistance, supervision and instruction for students, faculty and commercial visitors using the shared laboratories, optical microscopes, computers, scanners and other shared laboratory equipment. The MicroAnalytical Research Assistant will provide computer backup and file sharing maintenance and support for the MicroAnalytical Facility. Complete details and application procedure at http://academicjobsonline.org.
Duties: Sample Preparation/Coating/Sputtering:
50% Sample Preparation for MicroAnalytical Facility: Cutting, grinding, cleaving, mounting, embedding, and polishing and coating of a wide variety of samples for use in the Microanalytical Facility. Both grain mounts, polished thick and thin sections and standard materials for calibration of the instruments. Vibratory polishing and etching for EBSD samples. Coating using a variety of sputter and evaporation coaters.
Sample preparation using tripod polishing, ultra-microtome, FIB and Electron Beam Lithography will also be required on occasion.
Instrument operation for MicroAnalytical:
30% Instrument Operation for MicroAnalytical Facility: Operate variable pressure FEG SEM and electron microprobes for imaging, mapping and quantitative analysis on a wide variety of samples and materials including thin films, archeological samples, geological samples, and photo-voltaic, semi-conductor, thermo-electric, super conductor and other bulk materials.
Operate energy dispersive spectrometers (EDS), electron backscatter detector (EBSD), cathodo-luminescence (CL) and utilize Peltier cold and hot stages for in-situ SEM experiments.
General facility support and assistance:
10% Computer Backup and Laboratory Maintenance: Manage instrument and shared computers in MicroAnalytical facility. Perform operating system updates, anti-virus updates, file maintenance, access security, user management on secure data servers, purchase parts and consumables for the MicroAnalytical Facility and Inorganic Preparation Laboratory as needed. Work with on-site instrument engineer and electronic engineer as necessary to assist in maintaining all laboratory equipment.
10% Tours, Workshops and Outreach: Conduct technical tours of the facility for community, industry, prospective student and other visitors. Support middle and high school and community college outreach efforts. Organize and support semi-annual workshops and topical conferences for the MicroAnalytical Facility. Other duties as assigned.
Qualifications: Bachelor's degree in a science related field and three years of experience in a laboratory and microscopy related field or Master's degree in a science related field and one year experience in a laboratory and microscopy related field.
Preferred ability in: Experience in sample preparation for EPMA and SEM, including epoxies, adhesives, vacuum impregnation, hot plate mounting with Petropoxy, thin sectioning, polishing with water/alcohol based diamond abrasives.
Operation of metallic/carbon evaporators and sputter coaters, fume hoods, spinners, etchers, and knowledge of proper handling and storing of chemicals.
Technical/scientific programming and computing management and applications. Norton Ghost for automated backup of data storage.
Demonstrated ability in: Following prescribed procedures, communicate effectively verbally and in writing.
Providing efficient and professional laboratory assistance in a fast paced environment. Effectively managing and prioritizing competing demands and keeping shared laboratory facilities at high levels of organization, cleanliness and safety levels. Absorbing a high level of technical details quickly.
The successful candidate will have the ability to work effectively with faculty, staff and students from a variety of diverse backgrounds.
Salary: Salary will be commensurate with qualifications and experience. This is a 0.5 FTE position. There is the possibility that the FTE may increase in the future depending on funding, performance and departmental need.
Application: http://academicjobsonline.org
Please include contact information for three professional references. Review of applications will begin September 27, 2011, and continue until a sufficient pool of qualified applicants is obtained or until the position is filled. This announcement is available in alternate formats upon request. If you are a qualified applicant with a disability and need accommodation with the application process, please call (541) 346-3159. If you need accommodation for the interview, please notify the Business Affairs Office if an interview is scheduled.
An Equal-Opportunity, Affirmative-Action Institution Committed to Cultural Diversity and Compliance with the Americans with Disabilities Act.
John Donovan Director- Microanalytical Facility CAMCOR-UofO 541-346-4632 donovan-at-uoregon.edu www.camcor.uoregon.edu www.epmalab.uoregon.edu
We used to charge out to our users for LN2 usage and determined that if we charged for less than 10 litres (up to 10 litres) we lost money. Funny enough people stopped asking for LN2.
We average a certain amount per day for our instruments and spread that cost over the range of instrument and our users do not see that amount on their bill. Some days we lose a little money and others we make money.
We got out of the nickel and dime charging a few years ago and it really streamlined our accounting.
Good luck.
Garnet
-- Garnet Martens Research Manager BioImaging Facility 6270 University Blvd Vancouver, BC V6T 1Z4 CANADA
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both parmiterd-at-mail.nih.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: parmiterd-at-mail.nih.gov Name: David
Organization: SAIC-Frederick
Title-Subject: [Filtered] X-Ray Diffraction Book
Message: Hello -
I'd like to know if anyone can recommend me a book on X-Ray diffraction. Specifically I'm looking for something that would include an index of d-values and such.
While I know there are archives which are constantly updated available through various groups, this is not something that I have a couple thousand dollars to spend on, and it is not 100% necessary for it to be 100% up-to-date.
Any help which can be offered will be appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kosel-at-nd.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kosel-at-nd.edu Name: Tom Kosel
Organization: Univ. Notre Dame
Title-Subject: [Filtered] TEM position available
Message: Hello -
A position is available for a TEM operator/manager for the FEI Titan 80-300 at Notre Dame, as described below. Please use the web addresses below, rather than replying to me.
Tom Kosel University of Notre Dame
Lab Manager for Transmission Electron Microscopy within the Notre Dame Integrated Imaging Facility
The University of Notre Dame has recently established the Integrated Imaging Facility with an integrated suite of state-of-the-art optical microscopes, electron beam microscopes, and small animal imaging stations. To learn more about the facility, visit http://ndiif.nd.edu. We seek a lab manager for the newly acquired FEI Titan 80-300 transmission electron microscope, equipped with EDS and EELS (Gatan Imaage Filter). It is a non-tenure position with renewable 12-month appointments. The successful candidate will have a strong publication record and considerable experience operating transmission electron microscopes. The position involves extensive collaboration with various research groups in the Colleges of Science and Engineering, and also the possibility of independent research. Ongoing research includes TEM of cross-sectional semiconductor specimens, nanoparticles and nanowires, graphene, etc. Biological TEM is done by an existing staff member. Complete specimen preparation facilities for materials science include an FEI Helios focused-ion beam (FIB) as well as ion milling, wedge polishing etc.
Please apply online at http://ND.jobs to Job #11437 or visit http://jobs.nd.edu/applicants/Central?quickFind=57475. For additional information about working at the University of Notre Dame and various benefits available to employees, please visit http://hr.nd.edu/why-nd.
The University of Notre Dame is committed to diversity (http://diversity.nd.edu/) in its staff, faculty, and student body. As such, we strongly encourage applications from members of minority groups, women, veterans, individuals with disabilities, and others who will enhance our community. The University of Notre Dame, an international Catholic research university, is an equal opportunity/affirmative action employer.
-at- Steve: unfortunately the problem is, at least, a cracked end. Maybe also a damage in an electric line inside the cable, but for sure a big crack in the teflon end.
-at- Justin and Steve: however, if not able to find a spare part, your suggestions will be very useful.
-at- Valery: at the moment we're not yet so discouraged to think of purchasing a new SEM. But before getting like that, we'll get in touch with you.
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tjj-at-stowers.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tjj-at-stowers.org Name: Teri Johnson
Organization: Stowers Institute for Medical Research
Title-Subject: [Filtered] Head, Histology and Electron Microscopy position open
Message: The Stowers Institute for Medical Research has an opening for a Head of Histology and Electron Microscopy to oversee expert delivery of the highest quality service for the detection of gene and protein expression in tissue samples; histochemical staining; sample fixation; routine histology; and ultra-structural analysis.
Primary responsibilities include maintaining the current status of projects and resolving issues in the EM and Histology labs; actively promoting team interaction and participation; monitoring workload and turn-around time through the LIMS system; providing oversight and feedback on complex or non-routine projects; monitoring usage of services; responding appropriately to unexpected peaks in workload and service requests; maintaining effective communication with all members of the Institute; troubleshooting problems and communicating appropriately with Principal Investigators and the scientific staff, administration, and other core facility personnel as needed; making formal and informal presentations on Core Center services; ensuring continuing education of staff through workshops, webinars, lab meetings, and email communications; and reading professional journals and other sources to stay current in Histologic and EM techniques.
In addition to outstanding communication skills and the ability to effectively multi-task in a team-oriented environment, the successful candidate will have QIHC (ASCP) Qualification, and HT or HTL certification. Previous management or supervisory experience is preferred.
Minimum requirements include an undergraduate degree in the sciences; excellent knowledge of histologic technique to include fixation, processing, histochemical staining, immunohistochemical staining, microtomy (to include cryostat, paraffin, and plastic), and ultramicrotomy; experience in a research environment with animal model histology; and the ability to apply good judgement to troubleshooting, problem solving, and staff management. Five to ten years relevant Histology and/or Electron Microscopy experience in lieu of education may be considered.
To learn more about the Institute and its mission visit www.stowers.org
To apply send resume and cover letter to careers-at-stowers.org
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bai-at-ceilabs.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bai-at-ceilabs.com Name: Tianbao Bai, Ph.D., CIH
Organization: Carolina Environmental, Inc.
Title-Subject: [Filtered] TEM Asbestos Lab Manager Position
Message: Hi, all.
We have a opening for our TEM asbestos lab manager position. We are located in Cary, North Carolina by Research Triangle Park. Minimum B.S. degree, M.S. preferred. Three years of asbestos TEM lab experiences are required. Please response directly to my email. Login Host: 74.239.161.166 ---------------------------------------------------------------------------
==============================Original Headers============================== 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Sep 21 19:47:17 2011 8, 25 -- Received: from znl.com ([206.69.208.20]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8M0lHkG004418 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Sep 2011 19:47:17 -0500 8, 25 -- Received: from localhost (localhost [127.0.0.1]) 8, 25 -- by znl.com (Postfix) with ESMTP id D4E7C414E70 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Sep 2011 19:47:17 -0500 (CDT) 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 8, 25 -- Received: from znl.com ([127.0.0.1]) 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 8, 25 -- with ESMTP id 4ihAHVmbanXv for {microscopy-at-microscopy.com} ; 8, 25 -- Wed, 21 Sep 2011 19:47:17 -0500 (CDT) 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 8, 25 -- by znl.com (Postfix) with ESMTPA id 59AF5414E65 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 21 Sep 2011 19:47:17 -0500 (CDT) 8, 25 -- Message-ID: {4E7A8595.2090107-at-microscopy.com} 8, 25 -- Date: Wed, 21 Sep 2011 19:47:17 -0500 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 8, 25 -- Reply-To: bai-at-ceilabs.com 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 8, 25 -- MIME-Version: 1.0 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 8, 25 -- Subject: viaWWW:TEM Asbestos Lab Manager Position 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The classic text I studied was by B.D. Cullity, but I suspect it is out of print and Amazon has only 10 copies left. However, if you search in Amazon on the words "X-Ray Diffraction", you will see a lot of newer texts along with reader's reviews of those texts. Beware, do it sitting down-the prices are shocking.
John Mardinly ASU } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } parmiterd-at-mail.nih.gov as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: parmiterd-at-mail.nih.gov Name: David } } Organization: SAIC-Frederick } } Title-Subject: [Filtered] X-Ray Diffraction Book } } Message: Hello - } } I'd like to know if anyone can recommend me a book on X-Ray diffraction. Specifically I'm looking } for something that would include an index of d-values and such. } } While I know there are archives which are constantly updated available through various groups, this } is not something that I have a couple thousand dollars to spend on, and it is not 100% necessary for } it to be 100% up-to-date. } } Any help which can be offered will be appreciated. } } Thanks! } } - David } } Login Host: 129.43.47.218 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Sep 19 21:53:36 2011 } 13, 25 -- Received: from znl.com ([206.69.208.20]) } 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8K2raVZ032487 } 13, 25 -- for {microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 21:53:36 -0500 } 13, 25 -- Received: from localhost (localhost [127.0.0.1]) } 13, 25 -- by znl.com (Postfix) with ESMTP id F354A41367B } 13, 25 -- for {microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 21:53:35 -0500 (CDT) } 13, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 25 -- Received: from znl.com ([127.0.0.1]) } 13, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 13, 25 -- with ESMTP id O6J4EAhMmB94 for {microscopy-at-microscopy.com} ; } 13, 25 -- Mon, 19 Sep 2011 21:53:30 -0500 (CDT) } 13, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 25 -- by znl.com (Postfix) with ESMTPA id 0B73D413670 } 13, 25 -- for {microscopy-at-microscopy.com} ; Mon, 19 Sep 2011 21:53:29 -0500 (CDT) } 13, 25 -- Message-ID: {4E780029.9070000-at-microscopy.com} } 13, 25 -- Date: Mon, 19 Sep 2011 21:53:29 -0500 } 13, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 13, 25 -- Reply-To: parmiterd-at-mail.nih.gov } 13, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 25 -- MIME-Version: 1.0 } 13, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 25 -- Subject: viaWWW:X-Ray Diffraction Book } 13, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 4, 41 -- From John.Mardinly-at-asu.edu Thu Sep 22 12:48:26 2011 4, 41 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 4, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8MHmPn0025545 4, 41 -- for {Microscopy-at-Microscopy.com} ; Thu, 22 Sep 2011 12:48:25 -0500 4, 41 -- X-ASG-Debug-ID: 1316713703-03efbe20382b8bd0002-FOsErg 4, 41 -- Received: from exhub2.asurite.ad.asu.edu (exhub2.asurite.ad.asu.edu [129.219.103.60]) by bcnet6.asu.edu with ESMTP id 7ZtWkOXYB7EHntm5 (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Thu, 22 Sep 2011 10:48:24 -0700 (MST) 4, 41 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 4, 41 -- X-Barracuda-Apparent-Source-IP: 129.219.103.60 4, 41 -- X-ASG-Whitelist: Client 4, 41 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.196) by 4, 41 -- exhub2.asurite.ad.asu.edu (129.219.103.60) with Microsoft SMTP Server id 4, 41 -- 8.2.255.0; Thu, 22 Sep 2011 10:48:23 -0700 4, 41 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 4, 41 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Thu, 22 Sep 4, 41 -- 2011 10:48:23 -0700 4, 41 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 4, 41 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Thu, 22 Sep 2011 4, 41 -- 17:48:23 +0000 4, 41 -- Subject: Re: [Microscopy] viaWWW:X-Ray Diffraction Book 4, 41 -- References: {201109200304.p8K34JFA015505-at-ns.microscopy.com} 4, 41 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW:X-Ray Diffraction Book 4, 41 -- From: John Mardinly {John.Mardinly-at-asu.edu} 4, 41 -- Content-Type: text/plain; charset="us-ascii" 4, 41 -- Thread-Topic: [Microscopy] viaWWW:X-Ray Diffraction Book 4, 41 -- Thread-Index: Acx5T9Kjzm/aodLJRha+uLo5wJgAwQ== 4, 41 -- In-Reply-To: {201109200304.p8K34JFA015505-at-ns.microscopy.com} 4, 41 -- Message-ID: {C55A6167-9E7F-41FA-B672-789880F57FC6-at-exchange.asu.edu} 4, 41 -- Date: Thu, 22 Sep 2011 10:53:01 -0700 4, 41 -- To: {microscopylistserver-noreply-at-Microscopy.com} , 4, 41 -- MSA Listserver 4, 41 -- {Microscopy-at-Microscopy.com} 4, 41 -- CC: {John.Mardinly-at-asu.edu} 4, 41 -- MIME-Version: 1.0 (iPad Mail 8G4) 4, 41 -- X-OriginalArrivalTime: 22 Sep 2011 17:48:23.0435 (UTC) FILETIME=[D2E2ADB0:01CC794F] 4, 41 -- X-Barracuda-Connect: exhub2.asurite.ad.asu.edu[129.219.103.60] 4, 41 -- X-Barracuda-Start-Time: 1316713704 4, 41 -- X-Barracuda-Encrypted: RC4-MD5 4, 41 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 4, 41 -- X-Virus-Scanned: by bsmtpd at asu.edu 4, 41 -- Content-Transfer-Encoding: 8bit 4, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8MHmPn0025545 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both m-johnson2-at-northwestern.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: m-johnson2-at-northwestern.edu Name: Mark Johnson
Organization: Northwestern Biomedical Engineering
Title-Subject: [Filtered] Position Available; Need QFDE Experience
Message: Age-related macular degeneration (AMD) is a debilitating disease of the elderly that can lead to blindness. Associated with this disease is an accumulation of lipids that occurs in and around Bruch's membrane, adjacent to the retina. This project involves an ultrastructure examination of Bruch's membrane in a mouse model of AMD to better characterize the pathogenesis of this disease. This study is a collaborative project with Duke University.
Quick-freeze/deep-etch (QFDE) is a morphological technique that preserves extracellular matrix in exquisite detail and allows structures , particularly lipids, to be visualized that are not well resolved using conventional transmission electron microscopy (TEM) techniques. QFDE and other lipid-preserving histochemical techniques will be used in this study.
Position is available for a post-doctoral research fellow (or Northwestern graduate student). Must have experience using QFDE.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nesmicroscopy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] NESM Fall Meeting Oct. 20th: Register Online Today!
Message: Hello Fellow Listserverites!
Happy First Day of Fall! What better way to bring in the new Fall season than to join NESM for our annual Fall Meeting on Oct. 20, 2011 at The Center for Biological Imaging, Harvard University, Cambridge. The meeting is composed of five concurrent afternoon workshops and a dinner meeting.
We are also proud to announce our new website nesmicroscopy.org! Follow the link to see more information about our Fall Meeting. http://www.nesmicroscopy.org/
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Mancuso
Organization: LBNL
Title-Subject: [Filtered] Nitric Acid and Methanol SOP
Message: Listers,
Does anyone have an SOP for handling Nitric Acid and Methanol (typically used in electro-polishing TEM samples)? I help manage and maintain the spec prep labs at NCEM/LBNL and have all the information to make an SOP for the use and neutralization procedure but if there is already one out in the community, I'd rather reference an existing document than create it from scratch. Thanks, Marissa Mancuso (formerly known as Marissa Libbee)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both christopher.a.meyer2-at-boeing.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: christopher.a.meyer2-at-boeing.com Name: Chris Meyer
Organization: Boeing
Title-Subject: [Filtered] EBSD on Tabletop SEM
Message: Hello,
Does anyone have experience with EBSD on a tabletop 15kV SEM? I am interested in their experience. Is this practical? Thanks.
Hello! I've got a question about image processing. A very common task for a microscopist is to measure the size of particles on a TEM image. More generally, one needs to get the size distribution. If the particles are round, we measure the diameter of each particle. If the particles are elongated, we treat each particle as an ellipse (ImageJ can do that). The question is: how we can define the term "size" in general? When we have a mixture of elongated particles, triangular particles, complex shapes and others, it is rather difficult to explain what is "size". Could you please comment on this question or advice some books/articles on the topic? If we treat an elongated particle as an ellipse, how to estimate the error? -- Sincerely yours, Dmitry Bagrov
==============================Original Headers============================== 1, 23 -- From dbagrov-at-gmail.com Sat Sep 24 09:43:41 2011 1, 23 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 1, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8OEhfCf022995 1, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Sep 2011 09:43:41 -0500 1, 23 -- Received: by vcbfo14 with SMTP id fo14so2468050vcb.0 1, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Sep 2011 07:43:40 -0700 (PDT) 1, 23 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 1, 23 -- d=gmail.com; s=gamma; 1, 23 -- h=mime-version:date:message-id:subject:from:to:content-type; 1, 23 -- bh=Y+bzWILWe1en2xjy6JBtWZJbpyfig7bIQA7f0Ep27UI=; 1, 23 -- b=CwReifNxKdisdhowJ+SJ9PP7tCXDxObwaNOdbWentKZkhqEp4uDkG5SNlTntvemzl0 1, 23 -- QUQUbsHd8s7C9Fl+XkpOXJ1KTM6oOm4kd0dereN44/VkykXr8AsIMAuACsbRDUf0c3qg 1, 23 -- IRD27D70B5FRaW2wc2LYJ2z0dPYiVw8xh87wo= 1, 23 -- MIME-Version: 1.0 1, 23 -- Received: by 10.52.21.226 with SMTP id y2mr3988225vde.332.1316875420870; Sat, 1, 23 -- 24 Sep 2011 07:43:40 -0700 (PDT) 1, 23 -- Received: by 10.52.115.6 with HTTP; Sat, 24 Sep 2011 07:43:40 -0700 (PDT) 1, 23 -- Date: Sat, 24 Sep 2011 18:43:40 +0400 1, 23 -- Message-ID: {CAGwsbbEfb2mDqARMWTvapgRSaiShEgKyBFw5Ey1Q_W+auoDfvg-at-mail.gmail.com} 1, 23 -- Subject: Image processing - What is "size of a particle"? 1, 23 -- From: Dmitry Bagrov {dbagrov-at-gmail.com} 1, 23 -- To: Microscopy-at-microscopy.com 1, 23 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Dmitry brings up a good point. Those of us in the lab involved in particle characterization by image analysis and other techniques often note the problem where our clients want a single number to describe a complex distribution of particles which vary in projected area and shape. We call it "mono-numerosis."
Even for something as simple as spheroidal particles, we will typically measure at least 1000 single particles (see below for some comments about agglomerate rejection) and plot the distribution of equivalent circular diameters. We typically compare these measured distributions to model normal or lognormal distributions. The broader the distribution, the more particles one needs to measure to get precise parameters for the distribution. See Masuda and Inoye, J. Chem Eng. Jpn,, 4(1), 60-66 (1971).
We have looked at edge rounding of silver halide grains by TEM. We have a model that fits the projected particle boundary to a model of a super-sphere. Anytime one fits a measured boundary to a model shape, one should always examine the residuals from the fit and account for the large discrepancies. We have also fit particle boundaries to a model eclipse. Again, we look at the fit residuals. We have also looked at particles of varying shapes (including triangles.) Computing Fourier Shape Descriptors helps here. See E. T. Bowman et al, Geotechnique, 51(6), 545-554 (2001)
The general problem comes down to measuring a "feature vector" for each "blob" the image analysis algorithm detects. One then needs to do some classification analysis to sort out errors and then classify the "single particles" by size and shape.
For us, the first step is usually rejection of agglomerates. Most single particles are "convex" - meaning no re-entrant segments. We typically compute the "maximum intrusion distance" by finding the closest point on the convex hull for each point on the "blob" boundary and then finding the point on the boundary with the largest distance to the convex hull. This is the maximum intrusion distance. This provides very reliable agglomerate detection.
Once we have rejected agglomerates, we look at the rest of the items in the "feature vector" measured for each blob. These quantities might be the equivalent circular diameter, some generic shape factors like the circularity, perhaps the ratio of Feret diameters, and some Fourier Shape Descriptors or some Gray Level Moments (see Hu, IRE Trans Info Theo, 8, 179-187 (1962) and Belksim et al., Pattern Recognition, 24(12), 1117-38 (1991) and Gonzalez and Woods, Digital Image Processing, 514-518 (1993)).
With a new problem, we typically make some training sets of particles that we think represent the different classes and look at the differences between and within the classes for elements in the feature vector to come up with some proposed classifiers. This generally involves a bit of work looking at scatter plots of proposed classifiers for the different training set classes.
I should note that we use the AnalySIS Five image processing software from Olympus-SIS. I have no financial interest in this other than being a satisfied customer. We have done a lot of custom programming to get the feature measures we need. Often measurements are computationally-intensive and once we have verified that a protype Imaging-C module works, we will move the computationally-intensive functions into a DLL that has been compiled with an optimizing compiler and use the functions in the wrapper Imaging-C modules.
Hope this helps.
Best Regards, John On Sep 24, 2011, at 10:43 AM, dbagrov-at-gmail.com wrote: } Hello! } I've got a question about image processing. A very common task for a } microscopist is to measure the size of particles on a TEM image. } More generally, one needs to get the size distribution. If the } particles are round, we measure the diameter of each particle. } If the particles are elongated, we treat each particle as an ellipse } (ImageJ can do that). The question is: how we can define the term } "size" in general? } When we have a mixture of elongated particles, triangular particles, } complex shapes and others, it is rather difficult to explain what is } "size". } Could you please comment on this question or advice some } books/articles on the topic? If we treat an elongated particle as an } ellipse, how to estimate the error? } -- } Sincerely yours, } Dmitry Bagrov
==============================Original Headers============================== 12, 23 -- From jrminter-at-rochester.rr.com Sat Sep 24 13:19:27 2011 12, 23 -- Received: from hrndva-omtalb.mail.rr.com (hrndva-omtalb.mail.rr.com [71.74.56.125]) 12, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8OIJQhL010186 12, 23 -- for {Microscopy-at-microscopy.com} ; Sat, 24 Sep 2011 13:19:27 -0500 12, 23 -- X-Authority-Analysis: v=1.1 cv=cSzO76bR5tCkfUT9bEmBgR3d7VUusRLeq08eKGxa4EU= c=1 sm=0 a=QuLfOthXyaoA:10 a=kj9zAlcOel0A:10 a=QOXvuJxCzNGPTHlfmoyVfA==:17 a=pGLkceISAAAA:8 a=39fLcnd1S63HwRkRX8kA:9 a=pLvQGDUrBtbnwWB8Ah0A:7 a=CjuIK1q_8ugA:10 a=HgJAlmHKddEA:10 a=3STKU7uxFGsA:10 a=MSl-tDqOz04A:10 a=QOXvuJxCzNGPTHlfmoyVfA==:117 12, 23 -- X-Cloudmark-Score: 0 12, 23 -- X-Originating-IP: 74.65.12.77 12, 23 -- Received: from [74.65.12.77] ([74.65.12.77:36123] helo=[10.0.1.4]) 12, 23 -- by hrndva-oedge04.mail.rr.com (envelope-from {jrminter-at-rochester.rr.com} ) 12, 23 -- (ecelerity 2.2.3.46 r()) with ESMTP 12, 23 -- id D9/4E-05514-E2F1E7E4; Sat, 24 Sep 2011 18:19:26 +0000 12, 23 -- Content-Type: text/plain; charset=us-ascii 12, 23 -- Mime-Version: 1.0 (Apple Message framework v1084) 12, 23 -- Subject: Re: [Microscopy] Image processing - What is "size of a particle"? 12, 23 -- From: John Minter {jrminter-at-rochester.rr.com} 12, 23 -- In-Reply-To: {201109241443.p8OEhndr023118-at-ns.microscopy.com} 12, 23 -- Date: Sat, 24 Sep 2011 14:19:25 -0400 12, 23 -- Message-Id: {0A4D67A4-36A8-4531-9648-7385E096930B-at-rochester.rr.com} 12, 23 -- References: {201109241443.p8OEhndr023118-at-ns.microscopy.com} 12, 23 -- To: Microscopy-at-microscopy.com 12, 23 -- X-Mailer: Apple Mail (2.1084) 12, 23 -- Content-Transfer-Encoding: 8bit 12, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8OIJQhL010186 ==============================End of - Headers==============================
This question (how to characterise particle size) is, at one level, no different to any other exercise in statistical summary: how to give as much information as possible with just one number (statistically, the first moment, or mean), or with just two numbers (first and second moments - mean and standard deviation), or with just three numbers (the first three moments, or mean, standard deviation and kurtosis), etc.
I appreciate that in saying this I am merely restating one of the many salient points made by John Minter but I think it is worth re-stating, as being arguably the last common ancestor before it is necessary to branch into domain-specific answers. JM gives a summary of one domain, to which it may be useful to add a summary for sedimentary petrography and optical microscopy.
Particle shape is most often of interest to petrographers not for the particles themselves but for understanding the void space between. Therefore, packing is usually the most important single parameter (first moment), for which size is little more than a proxy. Hence the equivalents to JM's single parameters are measures such as points on the scales of Krumbein, Rittenhouse, Harrel, Powers or Pilkey. These were developed when analysis was purely visual, with no computational aids. Measuring angles and lengths was a time-consuming process. Does that mean they are redundant now that we can quickly click on many points on an image of representative grains and hence calculate any number of moments of the particle size distribution? Not if we return to Dmitry's question: how to summarise the important characteristics of size and shape in a heterogeneous sample, using just one or two numbers. These comparator charts were developed by Krumbein and others not only because it was not easy to enumerate a representative sub-population of particles, or calculate their distribution, but also because they are good ways of describing the distribution: in effect, they are domain-specific first moments.
So, in summary, I believe that the answer to Dmitry's question is domain-specific, wherein again I am only repeating JM's contribution. But the next step is to ask the question: to what extent does this 2-D slice through a 3-D medium capture the information the end-user is seeking? Staying with sedimentary petrography, a key piece of information is pore connectivity, and hence the shape and size of pore throats. What can a 2D slice tell us about this? Robert Ehrlich has spent a large part of his life studying this and has provided an extensive literature that can be readily searched, and which it would be presumptuous of me to even try to summarise, but I believe it is an interesting question whether or not one is a petrographer.
Finally, one of the main reasons I subscribe to this newsgroup is to read the comments of workers in other application domains. Sometimes, what is routine in one area can be a new insight in another, so thanks to all those, like JM below, who take the time to answer questions.
Barrie Wells
==============================Original Headers============================== 7, 33 -- From Barrie.Wells-at-ConwyValley.com Sun Sep 25 06:25:43 2011 7, 33 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.17.9]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8PBPgZR026256 7, 33 -- for {Microscopy-at-microscopy.com} ; Sun, 25 Sep 2011 06:25:43 -0500 7, 33 -- Received: from TOSH07 (202.14.17.93.rev.sfr.net [93.17.14.202]) 7, 33 -- by mrelayeu.kundenserver.de (node=mreu3) with ESMTP (Nemesis) 7, 33 -- id 0Mh2kG-1QlsGa44p6-00MnlO; Sun, 25 Sep 2011 13:25:41 +0200 7, 33 -- Message-ID: {010801cc7b75$8d9b5860$8bc8a8c0-at-TOSH07} 7, 33 -- Reply-To: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} 7, 33 -- From: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} 7, 33 -- To: {Microscopy-at-microscopy.com} 7, 33 -- Cc: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} 7, 33 -- Subject: Re: Image processing - What is "size of a particle"? 7, 33 -- Date: Sun, 25 Sep 2011 12:23:25 +0100 7, 33 -- Organization: Conwy Valley Systems Limited 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Type: text/plain; 7, 33 -- format=flowed; 7, 33 -- charset="iso-8859-1"; 7, 33 -- reply-type=original 7, 33 -- Content-Transfer-Encoding: 7bit 7, 33 -- X-Priority: 3 7, 33 -- X-MSMail-Priority: Normal 7, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3664 7, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3664 7, 33 -- X-Provags-ID: V02:K0:CFpuRBwr0DRdjU2pf2okP8u20LCebrnkTB5b0VrWD7t 7, 33 -- d52Q/pIugRrlC7Rq5Hrk6taLegFNDMZkavafQxRnSNKF1SRwx+ 7, 33 -- cKRVer4GEHypOho7RdpPSdP6CzY46momjONprkvD1OpQ64PgCl 7, 33 -- GEJNnRJXK66MaiQR+nCm7Uhh+YuyFPhbBTQqa22Ie4EaAvIDi1 7, 33 -- tz4O5btHJvLJ70Lrp1H8E/4ENmA2rMimG50+Wlv8dkFXf3TLUk 7, 33 -- QSxoXeEgv+GcSXdtJI5+LNn2JckSGDaJWbMT6LqHK+NXcCKQVJ 7, 33 -- QeveRc6Yt4EgHACncytgxgX4YNxw6YiPlIaq8xKBJE0xxxBpAX 7, 33 -- ngl7F+X0Xy9fSmWXYY0M= ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dbagrov-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dbagrov-at-gmail.com Name: Mr. Dmitry Bagrov
Organization: Moscow State University
Title-Subject: [Filtered] What is "size of a particle"?
Message: Hello! I've got a question about image processing. A very common task for a microscopist is to measure the size of particles on a TEM image. More generally, one needs to get the size distribution. If the particles are round than we claim that we mesure the diameter of each particle. Sometimes we can treat each particle as an ellipse (ImageJ can do that). The question is: how we can define the term "size" in general? When we have a mixture of elongated particles, triangular particles, complex shapes and others, it is rather difficult to explain what is "size". Could you please comment on this question or advice some books/articles on the topic?
Is there any way to distinguish between tempered and untempered martensite (in steels) from selected area diffraction? Are the d-spacings or hkl tell anything?
Hello! Many thanks to everyone who answered my question, I highly appreciate your help! Although the procedure of particle size measurement seems simple, it is very difficult to measure the sizes accurately. Since the task is extremely common, many of us face this problem of image processing/metrology. As for me, I come to understanding that I always have to keep in mind the accuracy of measurement that I need. E.g. if the desired accuracy is not too high, I can treat a hexagonal particle as an ellipse without loss of reliability. Thanks everyone! -- Sincerely yours, Dmitry Bagrov
2011/9/25 {Barrie.Wells-at-conwyvalley.com} : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This question (how to characterise particle size) is, at one level, no } different to any other exercise in statistical summary: how to give as much } information as possible with just one number (statistically, the first } moment, or mean), or with just two numbers (first and second moments - mean } and standard deviation), or with just three numbers (the first three } moments, or mean, standard deviation and kurtosis), etc. } } I appreciate that in saying this I am merely restating one of the many } salient points made by John Minter but I think it is worth re-stating, as } being arguably the last common ancestor before it is necessary to branch } into domain-specific answers. JM gives a summary of one domain, to which it } may be useful to add a summary for sedimentary petrography and optical } microscopy. } } Particle shape is most often of interest to petrographers not for the } particles themselves but for understanding the void space between. } Therefore, packing is usually the most important single parameter (first } moment), for which size is little more than a proxy. Hence the equivalents } to JM's single parameters are measures such as points on the scales of } Krumbein, Rittenhouse, Harrel, Powers or Pilkey. These were developed when } analysis was purely visual, with no computational aids. Measuring angles and } lengths was a time-consuming process. Does that mean they are redundant now } that we can quickly click on many points on an image of representative } grains and hence calculate any number of moments of the particle size } distribution? Not if we return to Dmitry's question: how to summarise the } important characteristics of size and shape in a heterogeneous sample, using } just one or two numbers. These comparator charts were developed by Krumbein } and others not only because it was not easy to enumerate a representative } sub-population of particles, or calculate their distribution, but also } because they are good ways of describing the distribution: in effect, they } are domain-specific first moments. } } So, in summary, I believe that the answer to Dmitry's question is } domain-specific, wherein again I am only repeating JM's contribution. But } the next step is to ask the question: to what extent does this 2-D slice } through a 3-D medium capture the information the end-user is seeking? } Staying with sedimentary petrography, a key piece of information is pore } connectivity, and hence the shape and size of pore throats. What can a 2D } slice tell us about this? Robert Ehrlich has spent a large part of his life } studying this and has provided an extensive literature that can be readily } searched, and which it would be presumptuous of me to even try to summarise, } but I believe it is an interesting question whether or not one is a } petrographer. } } Finally, one of the main reasons I subscribe to this newsgroup is to read } the comments of workers in other application domains. Sometimes, what is } routine in one area can be a new insight in another, so thanks to all those, } like JM below, who take the time to answer questions. } } Barrie Wells } } } ==============================Original Headers============================== } 7, 33 -- From Barrie.Wells-at-ConwyValley.com Sun Sep 25 06:25:43 2011 } 7, 33 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.17.9]) } 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8PBPgZR026256 } 7, 33 -- for {Microscopy-at-microscopy.com} ; Sun, 25 Sep 2011 06:25:43 -0500 } 7, 33 -- Received: from TOSH07 (202.14.17.93.rev.sfr.net [93.17.14.202]) } 7, 33 -- by mrelayeu.kundenserver.de (node=mreu3) with ESMTP (Nemesis) } 7, 33 -- id 0Mh2kG-1QlsGa44p6-00MnlO; Sun, 25 Sep 2011 13:25:41 +0200 } 7, 33 -- Message-ID: {010801cc7b75$8d9b5860$8bc8a8c0-at-TOSH07} } 7, 33 -- Reply-To: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} } 7, 33 -- From: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} } 7, 33 -- To: {Microscopy-at-microscopy.com} } 7, 33 -- Cc: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} } 7, 33 -- Subject: Re: Image processing - What is "size of a particle"? } 7, 33 -- Date: Sun, 25 Sep 2011 12:23:25 +0100 } 7, 33 -- Organization: Conwy Valley Systems Limited } 7, 33 -- MIME-Version: 1.0 } 7, 33 -- Content-Type: text/plain; } 7, 33 -- format=flowed; } 7, 33 -- charset="iso-8859-1"; } 7, 33 -- reply-type=original } 7, 33 -- Content-Transfer-Encoding: 7bit } 7, 33 -- X-Priority: 3 } 7, 33 -- X-MSMail-Priority: Normal } 7, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3664 } 7, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3664 } 7, 33 -- X-Provags-ID: V02:K0:CFpuRBwr0DRdjU2pf2okP8u20LCebrnkTB5b0VrWD7t } 7, 33 -- d52Q/pIugRrlC7Rq5Hrk6taLegFNDMZkavafQxRnSNKF1SRwx+ } 7, 33 -- cKRVer4GEHypOho7RdpPSdP6CzY46momjONprkvD1OpQ64PgCl } 7, 33 -- GEJNnRJXK66MaiQR+nCm7Uhh+YuyFPhbBTQqa22Ie4EaAvIDi1 } 7, 33 -- tz4O5btHJvLJ70Lrp1H8E/4ENmA2rMimG50+Wlv8dkFXf3TLUk } 7, 33 -- QSxoXeEgv+GcSXdtJI5+LNn2JckSGDaJWbMT6LqHK+NXcCKQVJ } 7, 33 -- QeveRc6Yt4EgHACncytgxgX4YNxw6YiPlIaq8xKBJE0xxxBpAX } 7, 33 -- ngl7F+X0Xy9fSmWXYY0M= } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 29 -- From dbagrov-at-gmail.com Mon Sep 26 01:29:08 2011 6, 29 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8Q6T81e026212 6, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 01:29:08 -0500 6, 29 -- Received: by vcbfo14 with SMTP id fo14so3278118vcb.0 6, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 25 Sep 2011 23:29:08 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 6, 29 -- :cc:content-type:content-transfer-encoding; 6, 29 -- bh=BMD4OKIdlJX8QFG+QwKIo/3g5GBaTNYBABFm/xWHYa8=; 6, 29 -- b=tFAR7BeOV2NqvfcuGgA8rf3zPOTE8DLKQBo3Dd0RzXd66W/3Ix/fTY8Xp16E9IbtJh 6, 29 -- C3mBtqa1CHN7GSFW+BaLuHIFvkWo6CcaPNNUqwFli5fUiMfk74IZI7P6sAmC6n+FgIE2 6, 29 -- lwqeCclqP/d+G36RJwl6VMf85vX98RcaVJ+vk= 6, 29 -- MIME-Version: 1.0 6, 29 -- Received: by 10.52.88.227 with SMTP id bj3mr6009236vdb.64.1317018547256; Sun, 6, 29 -- 25 Sep 2011 23:29:07 -0700 (PDT) 6, 29 -- Received: by 10.52.115.6 with HTTP; Sun, 25 Sep 2011 23:29:07 -0700 (PDT) 6, 29 -- In-Reply-To: {201109251135.p8PBZKVA006739-at-ns.microscopy.com} 6, 29 -- References: {201109251135.p8PBZKVA006739-at-ns.microscopy.com} 6, 29 -- Date: Mon, 26 Sep 2011 10:29:07 +0400 6, 29 -- Message-ID: {CAGwsbbG_0SwYFTCq8Rk+JMcZtS+=irxidt2ZpB0qKzr0yafiOw-at-mail.gmail.com} 6, 29 -- Subject: Re: [Microscopy] Re: Image processing - What is "size of a particle"? 6, 29 -- From: Dmitry Bagrov {dbagrov-at-gmail.com} 6, 29 -- To: Barrie.Wells-at-conwyvalley.com 6, 29 -- Cc: Microscopy-at-microscopy.com 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8Q6T81e026212 ==============================End of - Headers==============================
You could certainly do that, but I don't understand why. You would make an a priori assumption about the shape of the particle. The best way to do this would be to use a calibrated image, then use a thresholding technique and let the software give you the accurate area or circumference or a diameter (max, min, average) of the particle. If that is not possible (for example if the contrast between particle and matrix is too low), you could try image enhancement techniques, or techniques developed for grain boundary analysis, which use more information than just intensity values.
As has been pointed out by John and Barrie, "size" is not a very precise parameter. It could mean "length" for one person, "area" for another. I think once you have defined exactly what you want to measure, you will be able to devise measurement techniques that give you better results. Keep in mind also that you are typically only looking at a 2D representation of a 3D object, and your measurements could be biased.
Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: dbagrov-at-gmail.com [mailto:dbagrov-at-gmail.com] Sent: Monday, September 26, 2011 12:38 AM To: mike.bode-at-resaltatech.com
Hello! Many thanks to everyone who answered my question, I highly appreciate your help! Although the procedure of particle size measurement seems simple, it is very difficult to measure the sizes accurately. Since the task is extremely common, many of us face this problem of image processing/metrology. As for me, I come to understanding that I always have to keep in mind the accuracy of measurement that I need. E.g. if the desired accuracy is not too high, I can treat a hexagonal particle as an ellipse without loss of reliability. Thanks everyone! -- Sincerely yours, Dmitry Bagrov
2011/9/25 {Barrie.Wells-at-conwyvalley.com} : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This question (how to characterise particle size) is, at one level, no } different to any other exercise in statistical summary: how to give as much } information as possible with just one number (statistically, the first } moment, or mean), or with just two numbers (first and second moments - mean } and standard deviation), or with just three numbers (the first three } moments, or mean, standard deviation and kurtosis), etc. } } I appreciate that in saying this I am merely restating one of the many } salient points made by John Minter but I think it is worth re-stating, as } being arguably the last common ancestor before it is necessary to branch } into domain-specific answers. JM gives a summary of one domain, to which it } may be useful to add a summary for sedimentary petrography and optical } microscopy. } } Particle shape is most often of interest to petrographers not for the } particles themselves but for understanding the void space between. } Therefore, packing is usually the most important single parameter (first } moment), for which size is little more than a proxy. Hence the equivalents } to JM's single parameters are measures such as points on the scales of } Krumbein, Rittenhouse, Harrel, Powers or Pilkey. These were developed when } analysis was purely visual, with no computational aids. Measuring angles and } lengths was a time-consuming process. Does that mean they are redundant now } that we can quickly click on many points on an image of representative } grains and hence calculate any number of moments of the particle size } distribution? Not if we return to Dmitry's question: how to summarise the } important characteristics of size and shape in a heterogeneous sample, using } just one or two numbers. These comparator charts were developed by Krumbein } and others not only because it was not easy to enumerate a representative } sub-population of particles, or calculate their distribution, but also } because they are good ways of describing the distribution: in effect, they } are domain-specific first moments. } } So, in summary, I believe that the answer to Dmitry's question is } domain-specific, wherein again I am only repeating JM's contribution. But } the next step is to ask the question: to what extent does this 2-D slice } through a 3-D medium capture the information the end-user is seeking? } Staying with sedimentary petrography, a key piece of information is pore } connectivity, and hence the shape and size of pore throats. What can a 2D } slice tell us about this? Robert Ehrlich has spent a large part of his life } studying this and has provided an extensive literature that can be readily } searched, and which it would be presumptuous of me to even try to summarise, } but I believe it is an interesting question whether or not one is a } petrographer. } } Finally, one of the main reasons I subscribe to this newsgroup is to read } the comments of workers in other application domains. Sometimes, what is } routine in one area can be a new insight in another, so thanks to all those, } like JM below, who take the time to answer questions. } } Barrie Wells } } } ==============================Original Headers============================== } 7, 33 -- From Barrie.Wells-at-ConwyValley.com Sun Sep 25 06:25:43 2011 } 7, 33 -- Received: from moutng.kundenserver.de (moutng.kundenserver.de [212.227.17.9]) } 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8PBPgZR026256 } 7, 33 -- for {Microscopy-at-microscopy.com} ; Sun, 25 Sep 2011 06:25:43 -0500 } 7, 33 -- Received: from TOSH07 (202.14.17.93.rev.sfr.net [93.17.14.202]) } 7, 33 -- by mrelayeu.kundenserver.de (node=mreu3) with ESMTP (Nemesis) } 7, 33 -- id 0Mh2kG-1QlsGa44p6-00MnlO; Sun, 25 Sep 2011 13:25:41 +0200 } 7, 33 -- Message-ID: {010801cc7b75$8d9b5860$8bc8a8c0-at-TOSH07} } 7, 33 -- Reply-To: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} } 7, 33 -- From: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} } 7, 33 -- To: {Microscopy-at-microscopy.com} } 7, 33 -- Cc: "Barrie Wells" {Barrie.Wells-at-ConwyValley.com} } 7, 33 -- Subject: Re: Image processing - What is "size of a particle"? } 7, 33 -- Date: Sun, 25 Sep 2011 12:23:25 +0100 } 7, 33 -- Organization: Conwy Valley Systems Limited } 7, 33 -- MIME-Version: 1.0 } 7, 33 -- Content-Type: text/plain; } 7, 33 -- format=flowed; } 7, 33 -- charset="iso-8859-1"; } 7, 33 -- reply-type=original } 7, 33 -- Content-Transfer-Encoding: 7bit } 7, 33 -- X-Priority: 3 } 7, 33 -- X-MSMail-Priority: Normal } 7, 33 -- X-Mailer: Microsoft Outlook Express 6.00.2900.3664 } 7, 33 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3664 } 7, 33 -- X-Provags-ID: V02:K0:CFpuRBwr0DRdjU2pf2okP8u20LCebrnkTB5b0VrWD7t } 7, 33 -- d52Q/pIugRrlC7Rq5Hrk6taLegFNDMZkavafQxRnSNKF1SRwx+ } 7, 33 -- cKRVer4GEHypOho7RdpPSdP6CzY46momjONprkvD1OpQ64PgCl } 7, 33 -- GEJNnRJXK66MaiQR+nCm7Uhh+YuyFPhbBTQqa22Ie4EaAvIDi1 } 7, 33 -- tz4O5btHJvLJ70Lrp1H8E/4ENmA2rMimG50+Wlv8dkFXf3TLUk } 7, 33 -- QSxoXeEgv+GcSXdtJI5+LNn2JckSGDaJWbMT6LqHK+NXcCKQVJ } 7, 33 -- QeveRc6Yt4EgHACncytgxgX4YNxw6YiPlIaq8xKBJE0xxxBpAX } 7, 33 -- ngl7F+X0Xy9fSmWXYY0M= } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 29 -- From dbagrov-at-gmail.com Mon Sep 26 01:29:08 2011 6, 29 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8Q6T81e026212 6, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 01:29:08 -0500 6, 29 -- Received: by vcbfo14 with SMTP id fo14so3278118vcb.0 6, 29 -- for {Microscopy-at-microscopy.com} ; Sun, 25 Sep 2011 23:29:08 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 6, 29 -- :cc:content-type:content-transfer-encoding; 6, 29 -- bh=BMD4OKIdlJX8QFG+QwKIo/3g5GBaTNYBABFm/xWHYa8=; 6, 29 -- b=tFAR7BeOV2NqvfcuGgA8rf3zPOTE8DLKQBo3Dd0RzXd66W/3Ix/fTY8Xp16E9IbtJh 6, 29 -- C3mBtqa1CHN7GSFW+BaLuHIFvkWo6CcaPNNUqwFli5fUiMfk74IZI7P6sAmC6n+FgIE2 6, 29 -- lwqeCclqP/d+G36RJwl6VMf85vX98RcaVJ+vk= 6, 29 -- MIME-Version: 1.0 6, 29 -- Received: by 10.52.88.227 with SMTP id bj3mr6009236vdb.64.1317018547256; Sun, 6, 29 -- 25 Sep 2011 23:29:07 -0700 (PDT) 6, 29 -- Received: by 10.52.115.6 with HTTP; Sun, 25 Sep 2011 23:29:07 -0700 (PDT) 6, 29 -- In-Reply-To: {201109251135.p8PBZKVA006739-at-ns.microscopy.com} 6, 29 -- References: {201109251135.p8PBZKVA006739-at-ns.microscopy.com} 6, 29 -- Date: Mon, 26 Sep 2011 10:29:07 +0400 6, 29 -- Message-ID: {CAGwsbbG_0SwYFTCq8Rk+JMcZtS+=irxidt2ZpB0qKzr0yafiOw-at-mail.gmail.com} 6, 29 -- Subject: Re: [Microscopy] Re: Image processing - What is "size of a particle"? 6, 29 -- From: Dmitry Bagrov {dbagrov-at-gmail.com} 6, 29 -- To: Barrie.Wells-at-conwyvalley.com 6, 29 -- Cc: Microscopy-at-microscopy.com 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8Q6T81e026212 ==============================End of - Headers==============================
==============================Original Headers============================== 23, 32 -- From mike.bode-at-resaltatech.com Mon Sep 26 10:00:46 2011 23, 32 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 23, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QF0kGo030544 23, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 10:00:46 -0500 23, 32 -- Received: from c-98-245-223-115.hsd1.co.comcast.net ([98.245.223.115]:17104 helo=resdtpden01) 23, 32 -- by web38.justhost.com with esmtpa (Exim 4.69) 23, 32 -- (envelope-from {mike.bode-at-resaltatech.com} ) 23, 32 -- id 1R8Cfo-0001Pw-To; Mon, 26 Sep 2011 10:00:49 -0500 23, 32 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 23, 32 -- To: {Microscopy-at-microscopy.com} 23, 32 -- Cc: {dbagrov-at-gmail.com} 23, 32 -- References: {201109260637.p8Q6bvSb007122-at-ns.microscopy.com} 23, 32 -- In-Reply-To: {201109260637.p8Q6bvSb007122-at-ns.microscopy.com} 23, 32 -- Subject: RE: [Microscopy] Image processing - What is "size of a particle"? 23, 32 -- Date: Mon, 26 Sep 2011 09:00:41 -0600 23, 32 -- Message-ID: {000f01cc7c5d$10ec5bf0$32c513d0$-at-bode-at-resaltatech.com} 23, 32 -- MIME-Version: 1.0 23, 32 -- Content-Type: text/plain; 23, 32 -- charset="iso-8859-1" 23, 32 -- X-Mailer: Microsoft Office Outlook 12.0 23, 32 -- Thread-Index: Acx8FtRPhKOi9o9pQ76KWlQxztSyWAARJLkg 23, 32 -- Content-Language: en-us 23, 32 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 23, 32 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 23, 32 -- X-AntiAbuse: Original Domain - microscopy.com 23, 32 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 23, 32 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 23, 32 -- X-Source: 23, 32 -- X-Source-Args: 23, 32 -- X-Source-Dir: 23, 32 -- Content-Transfer-Encoding: 8bit 23, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8QF0kGo030544 ==============================End of - Headers==============================
We have an FEI Quanta 250 SEM. We frequently have power surges in the building and are looking to get an uninterruptible power supply (UPS) for it. We are looking at a Toshiba 1600XP series (3.6kVa). The sales rep is asking if we want it hard wired into the electrical or plugged into the wall; based on your experience, is one better than the other? Is the Toshiba a good model or are there others out there that are better?
Thanks in advance!
Michelle Shafer Whitman College shafermr-at-whitman.edu
==============================Original Headers============================== 4, 24 -- From shafermr-at-whitman.edu Mon Sep 26 12:40:05 2011 4, 24 -- Received: from mail.whitman.edu (mail.whitman.edu [199.89.174.121]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QHe5jj017223 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 12:40:05 -0500 4, 24 -- Received: from vams.whitman.edu (localhost.localdomain [127.0.0.1]) 4, 24 -- by mail.whitman.edu (Postfix) with SMTP id A36618041C 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 10:40:04 -0700 (PDT) 4, 24 -- Received: from mail.whitman.edu ([127.0.0.1]) 4, 24 -- by vams.whitman.edu ([10.1.1.203]) 4, 24 -- with SMTP (gateway) id A00C2FA0C90; Mon, 26 Sep 2011 10:40:04 -0700 4, 24 -- Received: from zcs-main.whitman.edu (zcs-main.whitman.edu [10.1.3.35]) 4, 24 -- by mail.whitman.edu (Postfix) with ESMTP id 9F0638041C 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 10:40:03 -0700 (PDT) 4, 24 -- Date: Mon, 26 Sep 2011 10:40:03 -0700 (PDT) 4, 24 -- From: Michelle Shafer {shafermr-at-whitman.edu} 4, 24 -- To: Microscopy {Microscopy-at-Microscopy.Com} 4, 24 -- Message-ID: {351707294.290982.1317058803606.JavaMail.root-at-zcs-main.whitman.edu} 4, 24 -- In-Reply-To: {421109250.290821.1317058392807.JavaMail.root-at-zcs-main.whitman.edu} 4, 24 -- Subject: Uninterruptible Power Supply for SEM - recommendations? 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; charset=utf-8 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Originating-IP: [10.4.1.171] 4, 24 -- X-Mailer: Zimbra 6.0.14_GA_2928 (ZimbraWebClient - FF3.0 (Mac)/6.0.14_GA_2928) ==============================End of - Headers==============================
If you have specimens thin enough for selected area diffraction, you may be able to tell the difference by examining them at high magnification. Martensite is a single phase material, usually with fine acicular grains. Tempered martensite is a two-phase structure consisting usually of very fine needle-shaped Fe3C carbide particles in a fine-grained BCC iron matrix. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
I would be very interested in the replies to this thread. I am moving into a section of the building undergoing active construction over the next decade and was to start looking into providing UPS for my three SEMs. Hadn't even started looking yet so the timing on this is wonderful.
Thanks,
Scott Whittaker Chief NMNH Imaging Manager SEM Lab Smithsonian Institution National Museum of Natural History PO Box 37012 MRC104 Washington DC 20013-7012 202-633-0891
Be prepared!! The Sem lab will be closing down and moving to First Floor West Wing most of the month of MARCH 2012. Dates subject to construction schedule. During that time the instrument calendars will be moved to outlook to take advantage of numerous features and improvements.
-----Original Message----- X-from: shafermr-at-whitman.edu [mailto:shafermr-at-whitman.edu] Sent: Monday, September 26, 2011 1:42 PM To: Whittaker, Scott
We have an FEI Quanta 250 SEM. We frequently have power surges in the building and are looking to get an uninterruptible power supply (UPS) for it. We are looking at a Toshiba 1600XP series (3.6kVa). The sales rep is asking if we want it hard wired into the electrical or plugged into the wall; based on your experience, is one better than the other? Is the Toshiba a good model or are there others out there that are better?
Thanks in advance!
Michelle Shafer Whitman College shafermr-at-whitman.edu
==============================Original Headers============================== 4, 24 -- From shafermr-at-whitman.edu Mon Sep 26 12:40:05 2011 4, 24 -- Received: from mail.whitman.edu (mail.whitman.edu [199.89.174.121]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QHe5jj017223 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 12:40:05 -0500 4, 24 -- Received: from vams.whitman.edu (localhost.localdomain [127.0.0.1]) 4, 24 -- by mail.whitman.edu (Postfix) with SMTP id A36618041C 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 10:40:04 -0700 (PDT) 4, 24 -- Received: from mail.whitman.edu ([127.0.0.1]) 4, 24 -- by vams.whitman.edu ([10.1.1.203]) 4, 24 -- with SMTP (gateway) id A00C2FA0C90; Mon, 26 Sep 2011 10:40:04 -0700 4, 24 -- Received: from zcs-main.whitman.edu (zcs-main.whitman.edu [10.1.3.35]) 4, 24 -- by mail.whitman.edu (Postfix) with ESMTP id 9F0638041C 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 10:40:03 -0700 (PDT) 4, 24 -- Date: Mon, 26 Sep 2011 10:40:03 -0700 (PDT) 4, 24 -- From: Michelle Shafer {shafermr-at-whitman.edu} 4, 24 -- To: Microscopy {Microscopy-at-Microscopy.Com} 4, 24 -- Message-ID: {351707294.290982.1317058803606.JavaMail.root-at-zcs-main.whitman.edu} 4, 24 -- In-Reply-To: {421109250.290821.1317058392807.JavaMail.root-at-zcs-main.whitman.edu} 4, 24 -- Subject: Uninterruptible Power Supply for SEM - recommendations? 4, 24 -- MIME-Version: 1.0 4, 24 -- Content-Type: text/plain; charset=utf-8 4, 24 -- Content-Transfer-Encoding: 7bit 4, 24 -- X-Originating-IP: [10.4.1.171] 4, 24 -- X-Mailer: Zimbra 6.0.14_GA_2928 (ZimbraWebClient - FF3.0 (Mac)/6.0.14_GA_2928) ==============================End of - Headers==============================
==============================Original Headers============================== 16, 31 -- From WHITTAKS-at-si.edu Mon Sep 26 13:17:36 2011 16, 31 -- Received: from si-mailout03.si.edu (si-mailout03.si.edu [160.111.103.177]) 16, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QIHapY018300 16, 31 -- for {microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 13:17:36 -0500 16, 31 -- Received: from SI-MSEHUB-N01.US.SINET.SI.EDU (si-msehubi-01.us.sinet.si.edu [160.111.49.142]) 16, 31 -- by si-mailout03.si.edu (Postfix) with ESMTP id 418005E46; 16, 31 -- Mon, 26 Sep 2011 14:17:36 -0400 (EDT) 16, 31 -- Received: from SI-MSEV03.US.SINET.SI.EDU ([fe80::58ac:1cff:44b0:524e]) by 16, 31 -- SI-MSEHUB-N01.US.SINET.SI.EDU ([fe80::34ec:9394:533a:63ad%11]) with mapi; 16, 31 -- Mon, 26 Sep 2011 14:17:36 -0400 16, 31 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu} 16, 31 -- To: "'shafermr-at-whitman.edu'" {shafermr-at-whitman.edu} , 16, 31 -- "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 16, 31 -- Date: Mon, 26 Sep 2011 14:17:34 -0400 16, 31 -- Subject: RE: [Microscopy] Uninterruptible Power Supply for SEM - 16, 31 -- recommendations? 16, 31 -- Thread-Topic: [Microscopy] Uninterruptible Power Supply for SEM - 16, 31 -- recommendations? 16, 31 -- Thread-Index: Acx8c4heH0ZVPASzTEy2p1p45h3zEgABJMYw 16, 31 -- Message-ID: {038C04650A93A14381E944A473695F619491FBEA13-at-SI-MSEV03.US.SINET.SI.EDU} 16, 31 -- References: {201109261741.p8QHfVoN019151-at-ns.microscopy.com} 16, 31 -- In-Reply-To: {201109261741.p8QHfVoN019151-at-ns.microscopy.com} 16, 31 -- Accept-Language: en-US 16, 31 -- Content-Language: en-US 16, 31 -- X-MS-Has-Attach: 16, 31 -- X-MS-TNEF-Correlator: 16, 31 -- acceptlanguage: en-US 16, 31 -- Content-Type: text/plain; charset="iso-8859-1" 16, 31 -- MIME-Version: 1.0 16, 31 -- Content-Transfer-Encoding: 8bit 16, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8QIHapY018300 ==============================End of - Headers==============================
We have an FEI dual-beam FIB and use a Toshiba 1600EP UPS system (it has 12 battery packs) - installed in 2006. Among all our instruments the FIB seems to survive the power outages better than most, so I'd say yes it is a good model. Ours is hard-wired and met local ordinances, just now I am replacing the batteries (after five years) - each battery pack is quite heavy and contains six 12 volt lead-acid batteries, The batteries are about $20 each.
Good luck
Robert Keyse
On 9/26/2011 1:49 PM, shafermr-at-whitman.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have an FEI Quanta 250 SEM. We frequently have power surges in the building and are looking to get an uninterruptible power supply (UPS) for it. We are looking at a Toshiba 1600XP series (3.6kVa). The sales rep is asking if we want it hard wired into the electrical or plugged into the wall; based on your experience, is one better than the other? Is the Toshiba a good model or are there others out there that are better? } } Thanks in advance! } } Michelle Shafer } Whitman College } shafermr-at-whitman.edu } } } ==============================Original Headers============================== } 4, 24 -- From shafermr-at-whitman.edu Mon Sep 26 12:40:05 2011 } 4, 24 -- Received: from mail.whitman.edu (mail.whitman.edu [199.89.174.121]) } 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QHe5jj017223 } 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 12:40:05 -0500 } 4, 24 -- Received: from vams.whitman.edu (localhost.localdomain [127.0.0.1]) } 4, 24 -- by mail.whitman.edu (Postfix) with SMTP id A36618041C } 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 10:40:04 -0700 (PDT) } 4, 24 -- Received: from mail.whitman.edu ([127.0.0.1]) } 4, 24 -- by vams.whitman.edu ([10.1.1.203]) } 4, 24 -- with SMTP (gateway) id A00C2FA0C90; Mon, 26 Sep 2011 10:40:04 -0700 } 4, 24 -- Received: from zcs-main.whitman.edu (zcs-main.whitman.edu [10.1.3.35]) } 4, 24 -- by mail.whitman.edu (Postfix) with ESMTP id 9F0638041C } 4, 24 -- for {Microscopy-at-Microscopy.Com} ; Mon, 26 Sep 2011 10:40:03 -0700 (PDT) } 4, 24 -- Date: Mon, 26 Sep 2011 10:40:03 -0700 (PDT) } 4, 24 -- From: Michelle Shafer {shafermr-at-whitman.edu} } 4, 24 -- To: Microscopy {Microscopy-at-Microscopy.Com} } 4, 24 -- Message-ID: {351707294.290982.1317058803606.JavaMail.root-at-zcs-main.whitman.edu} } 4, 24 -- In-Reply-To: {421109250.290821.1317058392807.JavaMail.root-at-zcs-main.whitman.edu} } 4, 24 -- Subject: Uninterruptible Power Supply for SEM - recommendations? } 4, 24 -- MIME-Version: 1.0 } 4, 24 -- Content-Type: text/plain; charset=utf-8 } 4, 24 -- Content-Transfer-Encoding: 7bit } 4, 24 -- X-Originating-IP: [10.4.1.171] } 4, 24 -- X-Mailer: Zimbra 6.0.14_GA_2928 (ZimbraWebClient - FF3.0 (Mac)/6.0.14_GA_2928) } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 22 -- From rok210-at-lehigh.edu Mon Sep 26 13:25:10 2011 5, 22 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QIP9aQ000309 5, 22 -- for {microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 13:25:10 -0500 5, 22 -- Received: from [128.180.55.15] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 5, 22 -- (authenticated bits=0) 5, 22 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id p8QIP8dn000838 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 5, 22 -- Mon, 26 Sep 2011 14:25:09 -0400 5, 22 -- Message-ID: {4E80C384.1010002-at-lehigh.edu} 5, 22 -- Date: Mon, 26 Sep 2011 14:25:08 -0400 5, 22 -- From: Robert Keyse {rok210-at-lehigh.edu} 5, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:6.0.2) Gecko/20110902 Thunderbird/6.0.2 5, 22 -- MIME-Version: 1.0 5, 22 -- To: shafermr-at-whitman.edu, microscopy-at-microscopy.com 5, 22 -- Subject: Re: [Microscopy] Uninterruptible Power Supply for SEM - recommendations? 5, 22 -- References: {201109261749.p8QHnwXI006184-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201109261749.p8QHnwXI006184-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Virus-Scanned: clamav-milter 0.97.2 at rain.cc.lehigh.edu 5, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dennis-at-houstonem.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dennis-at-houstonem.com Name: Dennis Manuel
Organization: Houston Electron Microscopy
Title-Subject: [Filtered] JEOL Service
Message: Any good JEOL service engineers in Houston? I have a JSM-6360LV with loose connection problem. Image is elongated in Y direction. Tapping door sometimes clears up and sometimes doesn't.
If you have a piece of the alloy large enough to perform a simple test on you can probably tell whether its Martensite or tempered Martensite by its physical properties. Just take a piece and bend it sharply and quickly (put it in a vise and strike it sharply sideways with a hammer). Martensite is a very brittle material, so if it's Martensite it will break. Tempered Martensite, on the other hand, is a very strong, tough material, so if that's what it is it will be very difficult to bend and will not break. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
Based on redundancy considerations, I would have UPS plugged into the wall and plug SEM into the UPS by the same plug. If UPS fails to the point that you can't use it in bypass mode, or if UPS should be removed for repairs, you will have possibility to re-plug SEM directly into the wall and have it back operational relatively quickly; in case of hard-wired installation you would need licensed electrician to temporarily bypass the UPS.
Use overrated twist-lock plugs for reliability and if Safety will permit then lock them mechanically to prevent accidental disconnection. -- Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/26/2011 1:49 PM, shafermr-at-whitman.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have an FEI Quanta 250 SEM. We frequently have power surges in the building and are looking to get an uninterruptible power supply (UPS) for it. We are looking at a Toshiba 1600XP series (3.6kVa). The sales rep is asking if we want it hard wired into the electrical or plugged into the wall; based on your experience, is one better than the other? Is the Toshiba a good model or are there others out there that are better? } } Thanks in advance! } } Michelle Shafer } Whitman College } shafermr-at-whitman.edu
could you please forward this email to anybody who might be interested in this position?
Thanks Robert
Research Assistant Professor Position in Aberration-Corrected Scanning Transmission Electron Microscopy
An Research Assistant Professor position is available in the Nanoscale Physics Group lead by Professor Robert F. Klie at the University of Illinois at Chicago to work on the application of atomic-resolution Z-contrast and annular bright-field imaging, as well as electron energy-loss spectroscopy (EELS) to functional nano-materials. The successful candidate will working with the group’s new aberration-corrected cold-field emission STEM (the JEOL ARM200-CF) with sub-Å and sub-eV resolution, equipped with several in-situ holders.
Candidates should have a Ph.D. in Physics, Materials Science or a related discipline. The position requires extensive experience in transmission electron microscopy and electron energy-loss spectroscopy. Experience working with aberration-corrected scanning transmission electron microscopy is also preferred. This position is available starting Nov 1, 2011, and remains open until it is filled. For fullest consideration apply by October 21, 2011. The salary will range between $45,000 and $70,000 depending on the level of experience and seniority.
Interested candidates can apply online at http://jobs.uic.edu. Please submit a cover letter, CV with a complete list of publications, and contact information of three professional references. Questions about the position should be directed to the search committee chair (Prof. Robert Klie: rfklie-at-uic.edu). UIC is an AA/EOE
-- Robert F. Klie, PhD Associate Professor University of Illinois at Chicago Department of Physics Chicago, IL 60607 Tel: 312-996-6064 Fax: 312-996-9016
==============================Original Headers============================== 11, 18 -- From rfklie-at-uic.edu Mon Sep 26 23:46:36 2011 11, 18 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8R4kah6012559 11, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 23:46:36 -0500 11, 18 -- Received: by vws16 with SMTP id 16so8449182vws.0 11, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 21:46:36 -0700 (PDT) 11, 18 -- MIME-Version: 1.0 11, 18 -- Received: by 10.52.91.97 with SMTP id cd1mr354898vdb.218.1317098795938; Mon, 11, 18 -- 26 Sep 2011 21:46:35 -0700 (PDT) 11, 18 -- Received: by 10.52.107.201 with HTTP; Mon, 26 Sep 2011 21:46:35 -0700 (PDT) 11, 18 -- Date: Mon, 26 Sep 2011 23:46:35 -0500 11, 18 -- Message-ID: {CAC9P5VYMVGyOnnjDOwi3SeOPO+L7=akW2N+4avB0grZu4XTW5A-at-mail.gmail.com} 11, 18 -- Subject: Job opening in aberration corrected STEM at UIC 11, 18 -- From: Robert Klie {rfklie-at-uic.edu} 11, 18 -- To: Microscopy-at-microscopy.com 11, 18 -- Content-Type: text/plain; charset=windows-1252 11, 18 -- Content-Transfer-Encoding: 8bit 11, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8R4kah6012559 ==============================End of - Headers==============================
John Mardinly ASU } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } dennis-at-houstonem.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: dennis-at-houstonem.com Name: Dennis Manuel } } Organization: Houston Electron Microscopy } } Title-Subject: [Filtered] JEOL Service } } Message: Any good JEOL service engineers in Houston? I have a JSM-6360LV with loose connection } problem. Image is elongated in Y direction. Tapping door sometimes clears up and sometimes doesn't. } } Login Host: 63.254.180.22 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Sep 26 18:09:10 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8QN9Aq4023438 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 18:09:10 -0500 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 16A04419C89 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 18:09:10 -0500 (CDT) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id 5jP9FggaMNNt for {microscopy-at-microscopy.com} ; } 8, 25 -- Mon, 26 Sep 2011 18:09:07 -0500 (CDT) } 8, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 39856419C7E } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 26 Sep 2011 18:09:07 -0500 (CDT) } 8, 25 -- Message-ID: {4E810613.6020009-at-microscopy.com} } 8, 25 -- Date: Mon, 26 Sep 2011 18:09:07 -0500 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: dennis-at-houstonem.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:JEOL Service } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 3, 41 -- From John.Mardinly-at-asu.edu Tue Sep 27 11:43:43 2011 3, 41 -- Received: from bcnet8.asu.edu (bcnet8.asu.edu [129.219.117.210]) 3, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8RGhgck010338 3, 41 -- for {Microscopy-at-Microscopy.com} ; Tue, 27 Sep 2011 11:43:43 -0500 3, 41 -- X-ASG-Debug-ID: 1317141820-039743126600a80001-FOsErg 3, 41 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet8.asu.edu with ESMTP id HxF1HUor6cvgWTNX (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Tue, 27 Sep 2011 09:43:40 -0700 (MST) 3, 41 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 3, 41 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 3, 41 -- X-ASG-Whitelist: Client 3, 41 -- Received: from EX06.asurite.ad.asu.edu (129.219.12.207) by 3, 41 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 3, 41 -- 8.2.255.0; Tue, 27 Sep 2011 09:43:40 -0700 3, 41 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 3, 41 -- EX06.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Tue, 27 Sep 3, 41 -- 2011 09:43:40 -0700 3, 41 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 3, 41 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Tue, 27 Sep 2011 3, 41 -- 16:43:39 +0000 3, 41 -- Subject: Re: [Microscopy] viaWWW:JEOL Service 3, 41 -- References: {201109262319.p8QNJNYo006235-at-ns.microscopy.com} 3, 41 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW:JEOL Service 3, 41 -- From: John Mardinly {John.Mardinly-at-asu.edu} 3, 41 -- Content-Type: text/plain; charset="us-ascii" 3, 41 -- Thread-Topic: [Microscopy] viaWWW:JEOL Service 3, 41 -- Thread-Index: Acx9NJw0YFZ6SREdSdO+EJ7MgMueGQ== 3, 41 -- In-Reply-To: {201109262319.p8QNJNYo006235-at-ns.microscopy.com} 3, 41 -- Message-ID: {CB362C33-EF25-48AF-B824-F7D45E73A484-at-exchange.asu.edu} 3, 41 -- Date: Tue, 27 Sep 2011 09:48:25 -0700 3, 41 -- To: {microscopylistserver-noreply-at-Microscopy.com} , 3, 41 -- MSA Listserver 3, 41 -- {Microscopy-at-Microscopy.com} 3, 41 -- CC: {John.Mardinly-at-asu.edu} 3, 41 -- MIME-Version: 1.0 (iPad Mail 8G4) 3, 41 -- X-OriginalArrivalTime: 27 Sep 2011 16:43:40.0111 (UTC) FILETIME=[9C4F35F0:01CC7D34] 3, 41 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 3, 41 -- X-Barracuda-Start-Time: 1317141820 3, 41 -- X-Barracuda-Encrypted: RC4-MD5 3, 41 -- X-Barracuda-URL: http://129.219.117.210:8000/cgi-mod/mark.cgi 3, 41 -- X-Virus-Scanned: by bsmtpd at asu.edu 3, 41 -- Content-Transfer-Encoding: 8bit 3, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8RGhgck010338 ==============================End of - Headers==============================
I am in charge in finding us a new carbon coater. I would like a carbon coater that also has an attached ion polisher. Does something like this exist? If so, who makes it? What sort of details should I look into?
Please respond to: ibarke2-at-uwo.ca
Thanks!
________________________________ Ivan R. Barker UWO Earth Sciences MSc ZAPLab Geochronology Research Technician T: 519-661-2111 ext. 88397 E: ibarke2-at-uwo.ca
==============================Original Headers============================== 6, 28 -- From ivan.r.barker-at-gmail.com Tue Sep 27 15:16:33 2011 6, 28 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8RKGXYa030194 6, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Sep 2011 15:16:33 -0500 6, 28 -- Received: by vcbfo14 with SMTP id fo14so5191703vcb.0 6, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Sep 2011 13:16:33 -0700 (PDT) 6, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 28 -- d=gmail.com; s=gamma; 6, 28 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 6, 28 -- :subject:to:content-type:content-transfer-encoding; 6, 28 -- bh=dFXuNfjUBUrSQNeJWDSgcT+WrsBG/W6bJ1Pvvf3O5UM=; 6, 28 -- b=OhLdOhF4LpdS6mHxe65ieRpr+1v6fBz6OoW8iKFIcwZOmVTvzpyu68Pm37M/Ja0Goo 6, 28 -- O8jExn1PmPa1i3IdHuNfm5I2qEaol0nZ6Dj0SGlmvrPEhYxayp/qb/afPyJAzKixtqk1 6, 28 -- mXhgG60ogJR3l2g2Se5S4leXaGNXTlYJO8SHA= 6, 28 -- Received: by 10.220.152.82 with SMTP id f18mr2381457vcw.138.1317154593184; 6, 28 -- Tue, 27 Sep 2011 13:16:33 -0700 (PDT) 6, 28 -- MIME-Version: 1.0 6, 28 -- Sender: ivan.r.barker-at-gmail.com 6, 28 -- Received: by 10.220.6.212 with HTTP; Tue, 27 Sep 2011 13:16:13 -0700 (PDT) 6, 28 -- From: Ivan Barker {ibarke2-at-uwo.ca} 6, 28 -- Date: Tue, 27 Sep 2011 16:16:13 -0400 6, 28 -- X-Google-Sender-Auth: vb9rU5fG9TPVr48sUa3Q3Czyi50 6, 28 -- Message-ID: {CAO25WkdCb69zjhWVG-1BQw==wm+R_jdQAP+hg1Oib8EMjUuXwg-at-mail.gmail.com} 6, 28 -- Subject: SEM - Looking for a combo coater and ion polisher 6, 28 -- To: Microscopy-at-microscopy.com 6, 28 -- Content-Type: text/plain; charset=ISO-8859-1 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8RKGXYa030194 ==============================End of - Headers==============================
Your diffraction results suggest that you have only ferrite in the areas examined. If either cementite or martensite were present in any quantity I would expect you to see a complex array of spots/rings from them in addition to the BCC spots/rings of ferrite. Is there any chance that your surface material has been de-carburized? because I would also expect you to be able to see the fine carbide particles if it were simple tempered martensite.
Here are some lattice parameter data, for your reference: Ferrite is BCC with a unite cell size of about 2.866Å, Cementite (Fe3C) is orthorhombicv with a = 4.52, b = 5.09, and c = 6.74Å., while Martensite is BC tetragonal with a = b = 2.867 - 0.013x and c = 2.867 + 0.116x where x = wt. pct carbon. (Elements of X-ray Diffraction, by B. D. Cullity, Addison Wesley, 1959, Appendix 13 and Table A-13-2))
-- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 6, 17 -- From bigelow-at-umich.edu Tue Sep 27 15:52:41 2011 6, 17 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 6, 17 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8RKqf8f014763 6, 17 -- for {microscopy-at-microscopy.com} ; Tue, 27 Sep 2011 15:52:41 -0500 6, 17 -- Received: FROM [76.226.3.169] (adsl-76-226-3-169.dsl.sfldmi.sbcglobal.net [76.226.3.169]) 6, 17 -- By hellskitchen.mr.itd.umich.edu ID 4E823798.5EF10.21735 ; 6, 17 -- Authuser bigelow; 6, 17 -- 27 Sep 2011 16:52:40 EDT 6, 17 -- Mime-Version: 1.0 6, 17 -- Message-Id: {p06240801caa7e6e45372-at-[76.226.3.169]} 6, 17 -- Date: Tue, 27 Sep 2011 16:52:33 -0400 6, 17 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 6, 17 -- From: Wil Bigelow {bigelow-at-umich.edu} 6, 17 -- Subject: [Microscopy] RE:Martensite data 6, 17 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 6, 17 -- Content-Transfer-Encoding: 8bit 6, 17 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8RKqf8f014763 ==============================End of - Headers==============================
There are several manufacturers of ion beam sputter systems with etching and polishing options available on the market that will do what you want.
I'm a bit biased in saying that our IBS/e unit is the best available. With our system, you have the option of a focused high energy ion gun for a small area ion etching and polishing or a large area low energy Kaufman ion source that can ion treat up to a 2" diameter sample. This is perfect for petrographic samples. We have been able to ion polish the entire surfaces of two inch petrographic samples for EBSD analysis. We can sputter coat with our standard holder to a two inch diameter sample and up to a four inch diameter sample with our large area stage. With our standard high energy ion gun used for etching and polishing, the energy range is 2500 eV -10,000 eV. With the KDC-10 ion source, the energy range is 100 eV to 1000 eV. With both types of system, the angular range for polishing/etching is 0 to 90°, where ion polishing is in the high angle range 84°-90° (relative to the surface normal) and ion etching is usually done in the range of 45°-60° (relative to the surface normal).
In your posting, you say that you are interested in a carbon coater. You can ion beam sputter carbon and create a very good coating. However, when you have an ion beam sputter system, you can put down very controlled, thin, uniform coatings over highly topographic samples with other materials as well. For example, a 10Å layer of Ir, Cr, or W can be used at very high magnifications because these materials have very small grain size. A 10Å layer of Ir will work very well to eliminate charging from your samples and will not show up in the XEDS spectra unless very, very long acquisition times are used. We supply both carbon and iridium targets as standard with our system.
The combination of ion beam sputter coating and ion polishing/etching gives your laboratory a very versatile research instrument. The etching capability can be used for light microscopy as well. The ion beam sputter process can put down virtually any type of film that you might be interested in, not only those for microscopy. If your system is equipped with auxiliary gas, then you can do reactive ion sputtering and reactive sputter deposition as well.
Disclaimer: SBT manufacturers and sells the IBS/e Ion Beam Sputter and Etch system.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-----Original Message----- X-from: ibarke2-at-uwo.ca [mailto:ibarke2-at-uwo.ca] Sent: Tuesday, September 27, 2011 1:25 PM To: swalck-at-southbaytech.com
Hi there,
I am in charge in finding us a new carbon coater. I would like a carbon coater that also has an attached ion polisher. Does something like this exist? If so, who makes it? What sort of details should I look into?
Please respond to: ibarke2-at-uwo.ca
Thanks!
________________________________ Ivan R. Barker UWO Earth Sciences MSc ZAPLab Geochronology Research Technician T: 519-661-2111 ext. 88397 E: ibarke2-at-uwo.ca
==============================Original Headers============================== 6, 28 -- From ivan.r.barker-at-gmail.com Tue Sep 27 15:16:33 2011 6, 28 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8RKGXYa030194 6, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Sep 2011 15:16:33 -0500 6, 28 -- Received: by vcbfo14 with SMTP id fo14so5191703vcb.0 6, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 27 Sep 2011 13:16:33 -0700 (PDT) 6, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 28 -- d=gmail.com; s=gamma; 6, 28 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 6, 28 -- :subject:to:content-type:content-transfer-encoding; 6, 28 -- bh=dFXuNfjUBUrSQNeJWDSgcT+WrsBG/W6bJ1Pvvf3O5UM=; 6, 28 -- b=OhLdOhF4LpdS6mHxe65ieRpr+1v6fBz6OoW8iKFIcwZOmVTvzpyu68Pm37M/Ja0Goo 6, 28 -- O8jExn1PmPa1i3IdHuNfm5I2qEaol0nZ6Dj0SGlmvrPEhYxayp/qb/afPyJAzKixtqk1 6, 28 -- mXhgG60ogJR3l2g2Se5S4leXaGNXTlYJO8SHA= 6, 28 -- Received: by 10.220.152.82 with SMTP id f18mr2381457vcw.138.1317154593184; 6, 28 -- Tue, 27 Sep 2011 13:16:33 -0700 (PDT) 6, 28 -- MIME-Version: 1.0 6, 28 -- Sender: ivan.r.barker-at-gmail.com 6, 28 -- Received: by 10.220.6.212 with HTTP; Tue, 27 Sep 2011 13:16:13 -0700 (PDT) 6, 28 -- From: Ivan Barker {ibarke2-at-uwo.ca} 6, 28 -- Date: Tue, 27 Sep 2011 16:16:13 -0400 6, 28 -- X-Google-Sender-Auth: vb9rU5fG9TPVr48sUa3Q3Czyi50 6, 28 -- Message-ID: {CAO25WkdCb69zjhWVG-1BQw==wm+R_jdQAP+hg1Oib8EMjUuXwg-at-mail.gmail.com} 6, 28 -- Subject: SEM - Looking for a combo coater and ion polisher 6, 28 -- To: Microscopy-at-microscopy.com 6, 28 -- Content-Type: text/plain; charset=ISO-8859-1 6, 28 -- Content-Transfer-Encoding: 8bit 6, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8RKGXYa030194 ==============================End of - Headers==============================
==============================Original Headers============================== 24, 24 -- From swalck-at-southbaytech.com Tue Sep 27 16:08:02 2011 24, 24 -- Received: from relay02.pair.com (relay02.pair.com [209.68.5.16]) 24, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p8RL82aW031425 24, 24 -- for {microscopy-at-microscopy.com} ; Tue, 27 Sep 2011 16:08:02 -0500 24, 24 -- Received: (qmail 64765 invoked from network); 27 Sep 2011 21:08:01 -0000 24, 24 -- Received: from 99.154.21.202 (HELO swalckPC) (99.154.21.202) 24, 24 -- by relay02.pair.com with SMTP; 27 Sep 2011 21:08:01 -0000 24, 24 -- X-pair-Authenticated: 99.154.21.202 24, 24 -- From: "Scott Walck" {swalck-at-southbaytech.com} 24, 24 -- To: {microscopy-at-microscopy.com} 24, 24 -- Cc: {ibarke2-at-uwo.ca} 24, 24 -- References: {201109272024.p8RKOfi2009279-at-ns.microscopy.com} 24, 24 -- In-Reply-To: {201109272024.p8RKOfi2009279-at-ns.microscopy.com} 24, 24 -- Subject: RE: [Microscopy] SEM - Looking for a combo coater and ion polisher 24, 24 -- Date: Tue, 27 Sep 2011 14:13:26 -0700 24, 24 -- Message-ID: {008301cc7d5a$4d0fc6f0$e72f54d0$-at-com} 24, 24 -- MIME-Version: 1.0 24, 24 -- Content-Type: text/plain; 24, 24 -- charset="iso-8859-1" 24, 24 -- X-Mailer: Microsoft Office Outlook 12.0 24, 24 -- Thread-Index: Acx9U32bsDEcmF9XRS+9Y8p9n+/VLgAAtfPw 24, 24 -- Content-Language: en-us 24, 24 -- Content-Transfer-Encoding: 8bit 24, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8RL82aW031425 ==============================End of - Headers==============================
I am sending our 20 yr old EDS detector in for repair. It currently has a Be window, but I'd like to switch to a thin window. Is this safe, i.e. would the thin window be durable? The TEM is a JEOL 4000FX that we run at 300kV max.
Thanks in advance.
Owen Mills Michigan Tech University Houghton, MI
==============================Original Headers============================== 4, 20 -- From prvs=245ea213d=opmills-at-mtu.edu Wed Sep 28 11:52:44 2011 4, 20 -- Received: from mail.mtu.edu (mx1.mtu.edu [141.219.73.15]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8SGqiCk018417 4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Sep 2011 11:52:44 -0500 4, 20 -- X-IronPort-Anti-Spam-Filtered: true 4, 20 -- X-IronPort-Anti-Spam-Result: AvsEAKBQg06N20GI/2dsb2JhbABCqAp4ghSBfYgNmQeWUIkBhithBKUm 4, 20 -- X-IronPort-AV: E=Sophos;i="4.68,456,1312171200"; 4, 20 -- d="scan'208";a="452923795" 4, 20 -- Received: from e12-0626-fac.coe.ad.mtu.edu ([141.219.65.136]) 4, 20 -- by email.mtu.edu with ESMTP/TLS/AES128-SHA; 28 Sep 2011 12:52:43 -0400 4, 20 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 4, 20 -- Content-Type: text/plain; charset=us-ascii 4, 20 -- Subject: TEM Thin window EDS 4, 20 -- Date: Wed, 28 Sep 2011 12:52:43 -0400 4, 20 -- Message-Id: {0D7997E6-09EF-4DD5-A8F9-0E3DD01C5CA2-at-mtu.edu} 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 4, 20 -- X-Mailer: Apple Mail (2.1084) 4, 20 -- Content-Transfer-Encoding: 8bit 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8SGqiCk018417 ==============================End of - Headers==============================
After 28 years at SIU, I will be retiring as Director of the IMAGE facility at SIU on October 31, 2011.
My staff of 4 will also be leaving (retiring) by the end of the year. I believe the university intends to fill the Directorship and two technical positions. Please note that this is a research support (not faculty/teaching) position and is not tenured. Here is a summary of the position that will be advertised very shortly:
POSITION ANNOUNCEMENT INTEGRATED MICROSCOPY & GRAPHICS EXPERTISE (IMAGE) SOUTHERN ILLINOIS UNIVERSITY CARBONDALE
POSITION TITLE: Director, IMAGE (12 month term, administrative professional)
QUALIFICATIONS: Professional electron microscopist with a minimum of 4 years experience managing a centralized, service-oriented facility with multidisciplinary clients. Ph.D. (or equivalent professional experience) in one of the hard sciences. Thorough understanding of theory and application of scanning and transmission electron microscopy, as well as ancillary techniques such as: energy dispersive X-ray microanalysis, ultramicrotomy, scanning transmission electron microscopy, vacuum coating procedures (sputtering, thermal evaporation) and specimen preparation techniques for biological and non-biological specimens. Experience in problem solving (trouble-shooting) with advanced instrumentation. Familiarity with light microscopy (conventional and fluorescence), image analysis and atomic force microscopy. Working knowledge of graphics programs such as Photoshop, Illustrator, InDesign, Acrobat, Powerpoint for use in professional publications and large format posters. Able to work independently with minimal administrative oversight or support. Excellent organizational and communication skills (oral and written), as well as experience writing grant proposals.
DUTIES: Oversee all operational aspects of the central IMAGE facility. This includes: (1) insuring performance of major instrumentation by training of technologists in basic maintenance (filament replacement, aperture cleaning, alignment) and clients in proper use of equipment, (2) trouble-shooting problems with equipment and determining best method to resolve issues, (3) developing long-range plan to replace aging equipment by pursuing external funding, (4) overseeing accounting (billing) and inventory control, (5) serving on student advisory committees and teaching courses in electron microscopy, (6) advising faculty with research projects involving electron microscopy, (7) training clients in proper methods to prepare posters for professional meetings, (8) overseeing printing of large-format posters and routine maintenance of poster printer, (9) providing tours of the facility to local colleges, schools and potential clients.
MAJOR INSTRUMENTATION: FEI Quanta 450 field emission scanning electron microscope equipped with Oxford X-Max 50 SDD energy dispersive X-ray microanalysis system with STEM, SSBSED, low kV SSBSED and Everhart Thronley detectors; Hitachi H7650II transmission electron microscope with integrated AMT digital camera; Hitachi H7100 STEM and S2460N variable pressure SEM both interfaced to Noran Voyager III X-ray microanalysis system; Hitachi S570 conventional SEM upgraded to 4pi digital image capture system; Park Scientific atomic force microscope.
SUPPORT INSTRUMENTATION: 5 ultramicrotomes (2 Leica UC6, UltraCut E, MT2-B, LKB III), Desk II sputter coater, Denton high vacuum evaporator, modern stereomicroscopes, fluorescence and conventional light microscopes (all with digital image capture), diamond knives, glass knife makers. HP 5000PS large format poster printer.
LOCATION: All equipment is located in a modern, standalone building centrally located on the campus of SIUC. The building was designed by John Bozzola, specifically for high resolution microscopy. Presently, we are installing a gas powered generator and 8.5KW UPS battery system in the event of power failure.
SIUC is an affirmative action/equal opportunity employer that strives to enhance its ability to develop a diverse faculty and staff and to increase its potential to service a diverse student population. All applications are welcomed and encouraged and will receive consideration.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 14, 18 -- From bozzola-at-siu.edu Wed Sep 28 14:31:38 2011 14, 18 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 14, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8SJVcl9004789 14, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Sep 2011 14:31:38 -0500 14, 18 -- Received: by iaen33 with SMTP id n33so10185116iae.0 14, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Sep 2011 12:31:38 -0700 (PDT) 14, 18 -- MIME-Version: 1.0 14, 18 -- Received: by 10.231.8.35 with SMTP id f35mr13304172ibf.4.1317238298100; Wed, 14, 18 -- 28 Sep 2011 12:31:38 -0700 (PDT) 14, 18 -- Received: by 10.231.145.20 with HTTP; Wed, 28 Sep 2011 12:31:38 -0700 (PDT) 14, 18 -- Date: Wed, 28 Sep 2011 14:31:38 -0500 14, 18 -- Message-ID: {CAKeCJ9yMiRnmQarPOKo2zdjJygXz_M02Z-RCJa1_3UnuJJUQUw-at-mail.gmail.com} 14, 18 -- Subject: EM Director Position Open 14, 18 -- From: John Bozzola {bozzola-at-siu.edu} 14, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 14, 18 -- Content-Type: text/plain; charset=UTF-8 14, 18 -- Content-Transfer-Encoding: 8bit 14, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8SJVcl9004789 ==============================End of - Headers==============================
Owen, in our lab we have a couple of EDS detectors (one for TEM, one for SEM) both with thin window. Both of them are 12 year old, and our TEM is operated usually at 300 kV. In last years we have had evidences of a kind of vacuum connection between TEM and EDS, which might be due to a small damage in the EDS window, but even TEM service engineers are not completely sure. Nevertheless we have managed to work in this condition for the last 2-3 years without any particular problem. Until now no problem even with SEM EDS thin window.
direct phone = 041.234.67.26 e-mail = dcristofori[at]unive[dot]it
Universita' Ca' Foscari Venezia Dipartimento di Sc. Molecolari e Nanosistemi Lab. di Scienza e Tecnologia dei Materiali Via Torino, 155b I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Il 28/09/2011 19.00, opmills-at-mtu.edu ha scritto: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } All, } } I am sending our 20 yr old EDS detector in for repair. It currently has a Be window, but I'd like to switch to a thin window. Is this safe, i.e. would the thin window be durable? The TEM is a JEOL 4000FX that we run at 300kV max. } } Thanks in advance. } } Owen Mills } Michigan Tech University } Houghton, MI } } ==============================Original Headers============================== } 4, 20 -- From prvs=245ea213d=opmills-at-mtu.edu Wed Sep 28 11:52:44 2011 } 4, 20 -- Received: from mail.mtu.edu (mx1.mtu.edu [141.219.73.15]) } 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8SGqiCk018417 } 4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 28 Sep 2011 11:52:44 -0500 } 4, 20 -- X-IronPort-Anti-Spam-Filtered: true } 4, 20 -- X-IronPort-Anti-Spam-Result: AvsEAKBQg06N20GI/2dsb2JhbABCqAp4ghSBfYgNmQeWUIkBhithBKUm } 4, 20 -- X-IronPort-AV: E=Sophos;i="4.68,456,1312171200"; } 4, 20 -- d="scan'208";a="452923795" } 4, 20 -- Received: from e12-0626-fac.coe.ad.mtu.edu ([141.219.65.136]) } 4, 20 -- by email.mtu.edu with ESMTP/TLS/AES128-SHA; 28 Sep 2011 12:52:43 -0400 } 4, 20 -- From: "Owen P. Mills" {opmills-at-mtu.edu} } 4, 20 -- Content-Type: text/plain; charset=us-ascii } 4, 20 -- Subject: TEM Thin window EDS } 4, 20 -- Date: Wed, 28 Sep 2011 12:52:43 -0400 } 4, 20 -- Message-Id: {0D7997E6-09EF-4DD5-A8F9-0E3DD01C5CA2-at-mtu.edu} } 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 4, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) } 4, 20 -- X-Mailer: Apple Mail (2.1084) } 4, 20 -- Content-Transfer-Encoding: 8bit } 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8SGqiCk018417 } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both skperkins-at-vt.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: skperkins-at-vt.edu Name: Sandy Hancock
Organization: Laboratory for Neurotoxicity Studies, VA Tech
Title-Subject: [Filtered] Cleaning diamond knives
Message: Hi Everyone,
I am looking for feedback on how folks are cleaning their diamond knives these days.... specifically, what process are you using to clean/remove epoxy resin sections (unfortunately)dried on the back of the knife edge. Please reply directly to me (skperkin-at-vt.edu)and I will provide a summary for the group.
The Fall MSORV meeting will be held on Tuesday, October 25th at Tech Solve, 6705 Steger Dr., Cincinnati, OH 45237 from 3-7:30 PM.
The program has been organized by Dan Kremser, President-elect, and an agenda will be posted soon on the MSORV website, www.msorv.org. We are very excited to have MSA's President-elect, Dr. Janet Woodward, as our keynote speaker. Speaker abstracts will be posted on the website soon. Please check it out to get meeting details, directions, etc.
To attend, you will have to pre-register in order for us to provide the caterer with a head count and to provide a list of names for security at Tech Solve. There is a $15 registration fee to attend for professional non-members and guests and $5 for student non-members, but if you choose to join at the meeting, the registration fee will be waived. Registration fees will be collected at the door. MSORV members (student and professional) do not have to pay a registration fee, but everyone planning to attend must pre-register!
Please send an e-mail to Pam Lloyd, MSORV's Secretary, at pamela.lloyd.ctr-at-wpafb.af.mil by October 12, 2011 to confirm your attendance.
==============================Original Headers============================== 7, 33 -- From pamela.lloyd.ctr-at-wpafb.af.mil Fri Sep 30 11:28:31 2011 7, 33 -- Received: from afnoc.af.mil (wpafb-mail2.afnoc.af.mil [132.3.33.19]) 7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8UGSURM018442 7, 33 -- for {microscopy-at-microscopy.com} ; Fri, 30 Sep 2011 11:28:31 -0500 7, 33 -- Received: from ([131.28.29.209]) 7, 33 -- by WPAFB-MAIL2.afnoc.af.mil with SMTP id 5FWP5M1.106127284; 7, 33 -- Fri, 30 Sep 2011 16:28:28 +0000 7, 33 -- Received: from FOHMLRL154.enterprise.afmc.ds.af.mil (fohmlrl154.enterprise.afmc.ds.af.mil [131.27.17.164]) 7, 33 -- by fohfwb001.oh.afmc.af.mil with ESMTP id p8UGSNrs078593 7, 33 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Sep 2011 16:28:28 GMT 7, 33 -- X-AuditID: 831b119a-b7c8fae000001e55-bd-4e85ee2ccdb6 7, 33 -- Received: from FOHMLBH02.Enterprise.afmc.ds.af.mil ( [131.28.34.102]) 7, 33 -- by FOHMLRL154.enterprise.afmc.ds.af.mil (Symantec Brightmail Gateway) with SMTP id 4F.DB.07765.C2EE58E4; Fri, 30 Sep 2011 12:28:28 -0400 (EDT) 7, 33 -- Received: from VFOHMLAO13.Enterprise.afmc.ds.af.mil ([131.28.34.133]) by FOHMLBH02.Enterprise.afmc.ds.af.mil with Microsoft SMTPSVC(6.0.3790.4675); 7, 33 -- Fri, 30 Sep 2011 12:28:28 -0400 7, 33 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 7, 33 -- Content-class: urn:content-classes:message 7, 33 -- MIME-Version: 1.0 7, 33 -- Content-Type: text/plain; 7, 33 -- charset="us-ascii" 7, 33 -- Subject: MSORV Fall 2011 Meeting 7, 33 -- Date: Fri, 30 Sep 2011 12:28:27 -0400 7, 33 -- Message-ID: {29A7C5F65D54744CBFD97CD62A33DEE909130280-at-VFOHMLAO13.Enterprise.afmc.ds.af.mil} 7, 33 -- X-MS-Has-Attach: 7, 33 -- X-MS-TNEF-Correlator: 7, 33 -- Thread-Topic: MSORV Fall 2011 Meeting 7, 33 -- Thread-Index: Acx/jfu55oWU50U+SDKtGQC2Cy6oiw== 7, 33 -- From: "Lloyd, Pamela F CTR USAF AFMC AFRL/RXPJ" {Pamela.Lloyd.ctr-at-wpafb.af.mil} 7, 33 -- To: {Microscopy-at-microscopy.com} 7, 33 -- X-OriginalArrivalTime: 30 Sep 2011 16:28:28.0160 (UTC) FILETIME=[FBFBC800:01CC7F8D] 7, 33 -- X-Brightmail-Tracker: AAAAAA== 7, 33 -- Content-Transfer-Encoding: 8bit 7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p8UGSURM018442 ==============================End of - Headers==============================
I would like to make you aware of an upcoming theoretical and practical course that will be of interest to anyone who is working with immunocytochemistry and colloidal gold particles.
The course - Quantifying immunogold label on EM thin sections - will be held from April 5th to 7th, 2012 at the House Research Institute, Los Angeles, CA, USA.
The course will review the current state-of-the-art for the following approaches:
[1] A target molecule can be tested for preferential labeling by mapping the localization of gold particles across a set of cell compartments. [2] Data from wild-type and knockdown/knockout cells can be used to correct raw gold particle counts, estimate specific labeling densities and then test for preferential labeling. [3] The same antigen can be mapped in two or more groups of cells to test whether or not there are experimental shifts in compartment labeling patterns. [4] In multiple-labeling studies, different sizes of gold particle can be used to test whether or not different antigens co-localize in one or more compartments. [5] Absolute numbers of gold particles can be mapped over compartments at specific positions within polarized, oriented or dividing cells.
Each approach will be introduced in a lecture and then illustrated by means of a practical exercise.
For more details:
Contact Paul Webster
Paul Webster, Ph.D. House Research Institute 2100 West Third Street Los Angeles CA 90057 (213) 273 8026 pwebster-at-hei.org
Or visit: http://immunogold.houseresearch.org
==============================Original Headers============================== 11, 18 -- From PWebster-at-hei.org Fri Sep 30 17:03:14 2011 11, 18 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p8UM3D3H007186 11, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 30 Sep 2011 17:03:13 -0500 11, 18 -- Received: from 10.10.42.116 ([10.10.42.116]) by hi0sml1.hei.org ([10.10.40.106]) with Microsoft Exchange Server HTTP-DAV ; 11, 18 -- Fri, 30 Sep 2011 22:03:13 +0000 11, 18 -- User-Agent: Microsoft-Entourage/12.27.0.100910 11, 18 -- Date: Fri, 30 Sep 2011 15:03:13 -0700 11, 18 -- Subject: Biological EM Course Announcement 11, 18 -- From: "Webster, Paul" {pwebster-at-hei.org} 11, 18 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 18 -- Message-ID: {CAAB8AB1.169A1%pwebster-at-hei.org} 11, 18 -- Thread-Topic: Biological EM Course Announcement 11, 18 -- Thread-Index: Acx/vL97Q165Y7uQQU+sL8vZsGSjwg== 11, 18 -- Mime-version: 1.0 11, 18 -- Content-type: text/plain; 11, 18 -- charset="US-ASCII" 11, 18 -- Content-transfer-encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hradek-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hradek-at-yahoo.com Name: Gary Hradek
Organization: UCSF
Title-Subject: [Filtered] ultracut s repair
Message: My name is Gary Hradek and I work at UCSF. I am contacting you because like us at UCSF you may have an Ultracut S. We have been told that Leica no longer services the Ultracut S. I am hoping someone might have suggestions about servicing this very expensive and unique piece of equipment. Thanks, Gary Hradek
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Hi, We have two old Reichert-Jung Ultracut E, one of them needed servicing. We had it serviced by Leica although they said that the support service was not available any longer. They did excellent job fixing and servicing it. Dorota
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hradek-at-yahoo.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hradek-at-yahoo.com Name: Gary Hradek
Organization: ucsf
Title-Subject: [Filtered] ultracut s
Message: I should like to thank all who responded to our issue with our ultracut S. Our problem is electronic and the board required to repair it is obsolete. I have been doing histology for over 30 years and the ultracut S was one of the finest microtomes made and one of the easiest to learn on. Even a medical student could learn to cut good sections in a day. Doctors took a bit longer. If you have a working ultracut s I would suggest turning off the lights when not in use as the light circuit draws the bulk of the power and may cause heat to build up and cook the circuit boards. Many thanks to all who responded. Gary
If you could not source the replacement PCB and can't fine more local service, then I'd be more then happy to fix the board for you.
There is no such thing as "unrepairable", though sometimes restoration projects may get pricey.
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/3/2011 7:46 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } hradek-at-yahoo.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: hradek-at-yahoo.com Name: Gary Hradek } } Organization: ucsf } } Title-Subject: [Filtered] ultracut s } } Message: I should like to thank all who responded to our issue with our ultracut S. Our problem is } electronic and the board required to repair it is obsolete. I have been doing histology for over } 30 years and the ultracut S was one of the finest microtomes made and one of the easiest to learn } on. Even a medical student could learn to cut good sections in a day. Doctors took a bit } longer. If you have a working ultracut s I would suggest turning off the lights when not in use as } the light circuit draws the bulk of the power and may cause heat to build up and cook the circuit } boards. Many thanks to all who responded. Gary } } Login Host: 128.218.88.103 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Oct 3 18:44:47 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p93NilID032262 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2011 18:44:47 -0500 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id BD5B941F0CF } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2011 18:44:47 -0500 (CDT) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id GVw9ldmIScPe for {microscopy-at-microscopy.com} ; } 8, 25 -- Mon, 3 Oct 2011 18:44:46 -0500 (CDT) } 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id B224841F0C4 } 8, 25 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2011 18:44:46 -0500 (CDT) } 8, 25 -- Message-ID: {4E8A48EE.3060401-at-microscopy.com} } 8, 25 -- Date: Mon, 03 Oct 2011 18:44:46 -0500 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: hradek-at-yahoo.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:ultracut s microtome } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 42 -- From vray-at-partbeamsystech.com Mon Oct 3 19:29:47 2011 5, 42 -- Received: from nm2-vm0.bullet.mail.sp2.yahoo.com (nm2-vm0.bullet.mail.sp2.yahoo.com [98.139.91.248]) 5, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p940Tknv016795 5, 42 -- for {microscopy-at-microscopy.com} ; Mon, 3 Oct 2011 19:29:46 -0500 5, 42 -- Received: from [98.139.91.66] by nm2.bullet.mail.sp2.yahoo.com with NNFMP; 04 Oct 2011 00:29:46 -0000 5, 42 -- Received: from [98.139.91.38] by tm6.bullet.mail.sp2.yahoo.com with NNFMP; 04 Oct 2011 00:27:46 -0000 5, 42 -- Received: from [127.0.0.1] by omp1038.mail.sp2.yahoo.com with NNFMP; 04 Oct 2011 00:27:46 -0000 5, 42 -- X-Yahoo-Newman-Id: 12432.19738.bm-at-omp1038.mail.sp2.yahoo.com 5, 42 -- Received: (qmail 18480 invoked from network); 4 Oct 2011 00:27:45 -0000 5, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1317688065; bh=5sMyXuXkHlqMzSe1PE3hyBA7zP3vfB+R4y43efGpytM=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=f77VsxKv/U0rVQp+T+n14n2W4/VbP9q7sIJxCcl8xKSF5GtIpV+iqpN8A0Qb7d+qZAN/0YN2/d/I6teNlAHzoumSIsuDeDq4pot3N66daW3en14+C+SAYQpCUeKPC+kVBWaVOIMjoavydqz5kbCVL09MQ0su1BdIkmz4fvgkbyA= 5, 42 -- X-Yahoo-Newman-Property: ymail-3 5, 42 -- X-YMail-OSG: YoZ2Ol8VM1nhSgUdS4AaBohgIUDRYRUH.XQzijxL.Vh.ADL 5, 42 -- 9gwiTy3ivS1qY4nUAcKUKBAZXjUFkDr9Tp.msiIGUL6gTsVuVk86LEZ67J7l 5, 42 -- nfmZN2Rf3qB8fbujuKss2KXhoErPwfuqQcGpS3XPDMvujPgAT1vZGT8HOIp4 5, 42 -- IvBo0xnDj5FCUGbTWCe0PK1jCl6wnBGqkdbAE.82D9u3EyYmylhTpxRDGbX9 5, 42 -- WXPoP.VVJjVZYzgZEH_j9wRNr_DYjXpBNjDWyaBqnXcPUxO2cqMrgLr1JRrG 5, 42 -- 3kzWWN0d0MXiOdzZxP8vrYhbzOZ0Dl0gGVkc.9snv6y.m93nps_ZSQEbL73J 5, 42 -- AncF63nrOfri0JuR7yP2yE0heeuRHtayJ8928oU6Ir4zUx9YlIdy5sqZw7hW 5, 42 -- pur4n3fxtZUv6VfScVNsSQX5NN3d.2XG2Z7QaIxGM7qHBCqXm5lM5Un1vk0s 5, 42 -- zNX.kSpo6Vz4Anif0589hHjJUJaPRTxumQjSYtsEJASvRqsdvVqjDNCo777a 5, 42 -- _u4lKXYiyYIaE5EV.h5IlcnAtYFggW7SgDeo.6giJbHEIrCiSUxTP85EaF4J 5, 42 -- 5YmFKjja9j_.6DdDuor6._yYtWxhotB5tXXtRCN7uOluiB0FLPk.cSTWNFJ_ 5, 42 -- 0t7bXnB1KR627hXc2TuaSeyop_uivRGhvM.7rymVyKdRCTC90XJCStOkPV0c 5, 42 -- 3Rq4XUoWgP5q.xkQxkWMJqT3Y8klz9aX4Y0_qMoq5rlv_aMsQWygJnga9JjC 5, 42 -- SpjWo 5, 42 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 42 -- Received: from [192.168.2.3] (vray-at-75.68.231.25 with plain) 5, 42 -- by smtp112.biz.mail.sp1.yahoo.com with SMTP; 03 Oct 2011 17:27:45 -0700 PDT 5, 42 -- Message-ID: {4E8A5302.9040104-at-partbeamsystech.com} 5, 42 -- Date: Mon, 03 Oct 2011 20:27:46 -0400 5, 42 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 42 -- Reply-To: vray-at-partbeamsystech.com 5, 42 -- Organization: PBS&T 5, 42 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 5, 42 -- MIME-Version: 1.0 5, 42 -- To: hradek-at-yahoo.com 5, 42 -- CC: microscopy-at-microscopy.com 5, 42 -- Subject: Re: [Microscopy] viaWWW:ultracut s microtome 5, 42 -- References: {201110032346.p93Nk8uw001137-at-ns.microscopy.com} 5, 42 -- In-Reply-To: {201110032346.p93Nk8uw001137-at-ns.microscopy.com} 5, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yashasin-at-rocketmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: yashasin-at-rocketmail.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Au analyzing
Message: Hi all Analyzing the Magnetite and pyrite with EPMA SX100 shows 1 to 1.5% Au in some points, is it natural or may be wrong? also our condition is: 15Kv, 20 nA and beam size 3um. and in BSE pictures have not been observed any visible gold. How much is the high grade Au concentration in Magnetite and Pyrite? there are some results: Point Fe Co Ni Au Total 1 / 1 . 61.27 0.23 1.05 1.29 66.03 2 / 1 . 60.95 0.42 0.05 0.93 63.57 3 / 1 . 53.95 0 0 1.43 56.4
I would like to bring to your attention two postdoctoral position openings in my group, one in the field of scanning probe microscopy (including novel dynamics, multifrequency, thermomechanical, and other advanced probes) and the other in the field of growth and in-situ SPM of oxide materials. The details for these positions are available at: 1. https://www3.orau.gov/ORNL_TOppS/Posting/Details/206 2. https://www3.orau.gov/ORNL_TOppS/Posting/Details/207
Please contact me directly if interested, or forward this information to your graduate students and postdocs.
Yours Sergei V. Kalinin
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 8, 25 -- From sergei2-at-ornl.gov Tue Oct 4 08:23:07 2011 8, 25 -- Received: from emroute1.ornl.gov (emroute1.ornl.gov [160.91.4.119]) 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p94DN7cf020823 8, 25 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 08:23:07 -0500 8, 25 -- Received: from emroute1.ornl.gov ([127.0.0.1]) 8, 25 -- by emroute1.ornl.gov (PMDF V6.5-x6 #31890) 8, 25 -- with ESMTP id {0LSJ00BMIMI98E-at-emroute1.ornl.gov} for 8, 25 -- microscopy-at-microscopy.com; Tue, 04 Oct 2011 09:22:57 -0400 (EDT) 8, 25 -- Received: from CONVERSION-DAEMON.emroute1.ornl.gov by emroute1.ornl.gov 8, 25 -- (PMDF V6.5-x6 #31890) id {0LSJ00F01MI9NU-at-emroute1.ornl.gov} for 8, 25 -- microscopy-at-microscopy.com; Tue, 04 Oct 2011 09:22:57 -0400 (EDT) 8, 25 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 8, 25 -- by emroute1.ornl.gov (PMDF V6.5-x6 #31890) 8, 25 -- with ESMTPS id {0LSJ009XHMI9Y1-at-emroute1.ornl.gov} for 8, 25 -- microscopy-at-microscopy.com; Tue, 04 Oct 2011 09:22:57 -0400 (EDT) 8, 25 -- Date: Tue, 04 Oct 2011 09:23:05 -0400 8, 25 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 8, 25 -- Subject: Two postdoctoral positions - SPM and oxides 8, 25 -- To: microscopy-at-microscopy.com 8, 25 -- Message-id: {4E8B08B9.5010203-at-ornl.gov} 8, 25 -- MIME-version: 1.0 8, 25 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 8, 25 -- Content-transfer-encoding: 7bit 8, 25 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.22) 8, 25 -- Gecko/20110902 Lightning/1.0b2 Thunderbird/3.1.14 ==============================End of - Headers==============================
Magnetite is usually forms crystals in some matrix. Depending on origin of your sample it can contain gold in varied quantities. Gold is associated with magnetite and gold prospectors are often looking for magnetite as a gold companion.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Manager 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Tuesday, October 04, 2011 7:59 AM } To: Dusevich, Vladimir } Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: EMPA Au } analyzing } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both } yashasin-at-rocketmail.com as well as the MIcroscopy Listserver } ----------------------------------------------------------------------- } ---- } } Email: yashasin-at-rocketmail.com Name: Kazem } } Organization: IMPRC } } Title-Subject: [Filtered] Au analyzing } } Message: Hi all } Analyzing the Magnetite and pyrite with EPMA SX100 shows 1 to 1.5% Au } in some points, is it natural } or may be wrong? also our condition is: 15Kv, 20 nA and beam size 3um. } and in BSE pictures have not } been observed any visible gold. } How much is the high grade Au concentration in Magnetite and Pyrite? } there are some results: } Point Fe Co Ni Au Total } 1 / 1 . 61.27 0.23 1.05 1.29 66.03 } 2 / 1 . 60.95 0.42 0.05 0.93 63.57 } 3 / 1 . 53.95 0 0 1.43 56.4 } } best regards } Kazem } } Login Host: 94.183.106.107 } ----------------------------------------------------------------------- } ---- } } } } ==============================Original } Headers============================== } 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Oct 4 } 07:58:39 2011 } 9, 25 -- Received: from znl.com ([206.69.208.20]) } 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p94CwdiR003782 } 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 07:58:39 - } 0500 } 9, 25 -- Received: from localhost (localhost [127.0.0.1]) } 9, 25 -- by znl.com (Postfix) with ESMTP id 60FA941F885 } 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 07:58:39 } -0500 (CDT) } 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 9, 25 -- Received: from znl.com ([127.0.0.1]) } 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 9, 25 -- with ESMTP id 63PjSBKi2qxl for {microscopy-at-microscopy.com} ; } 9, 25 -- Tue, 4 Oct 2011 07:58:33 -0500 (CDT) } 9, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 9, 25 -- by znl.com (Postfix) with ESMTPA id 7BF8241F875 } 9, 25 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 07:58:33 } -0500 (CDT) } 9, 25 -- Message-ID: {4E8B02F9.5070608-at-microscopy.com} } 9, 25 -- Date: Tue, 04 Oct 2011 07:58:33 -0500 } 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 9, 25 -- Reply-To: yashasin-at-rocketmail.com } 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 9, 25 -- MIME-Version: 1.0 } 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 25 -- Subject: [Filtered] MicroscopyListserverviaWWW: EMPA Au } analyzing } 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 6, 31 -- From DusevichV-at-umkc.edu Tue Oct 4 09:02:41 2011 6, 31 -- Received: from mxtip01.um.umsystem.edu (mxtip01.um.umsystem.edu [209.106.229.20]) 6, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p94E2fTQ005851 6, 31 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 09:02:41 -0500 6, 31 -- X-IronPort-Anti-Spam-Filtered: true 6, 31 -- X-IronPort-Anti-Spam-Result: ArYAAOMQi07Raua1/2dsb2JhbABCmGePEoEFgVMBAQU6FDcEAgEIEQMBAQENCgEHCQcxARQJCAEBBAoJCBUEh2GubIkBgkuBRgGCL2EEh3idTg 6, 31 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) 6, 31 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 04 Oct 2011 09:02:39 -0500 6, 31 -- Received: from um-email06.um.umsystem.edu ([169.254.1.230]) by 6, 31 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Tue, 4 Oct 2011 6, 31 -- 09:02:39 -0500 6, 31 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 6, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 6, 31 -- Date: Tue, 4 Oct 2011 09:02:38 -0500 6, 31 -- Subject: RE: [Microscopy] [Filtered] MicroscopyListserverviaWWW: EMPA Au 6, 31 -- analyzing 6, 31 -- Thread-Topic: [Microscopy] [Filtered] MicroscopyListserverviaWWW: EMPA Au 6, 31 -- analyzing 6, 31 -- Thread-Index: AcyClWtahFqbSpC8QXantUNTsVNGOAACGIzA 6, 31 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3B790E6F9BC-at-UM-EMAIL06.um.umsystem.edu} 6, 31 -- References: {201110041259.p94CxCdK004526-at-ns.microscopy.com} 6, 31 -- In-Reply-To: {201110041259.p94CxCdK004526-at-ns.microscopy.com} 6, 31 -- Accept-Language: en-US 6, 31 -- Content-Language: en-US 6, 31 -- X-MS-Has-Attach: 6, 31 -- X-MS-TNEF-Correlator: 6, 31 -- acceptlanguage: en-US 6, 31 -- Content-Type: text/plain; charset="us-ascii" 6, 31 -- MIME-Version: 1.0 6, 31 -- Content-Transfer-Encoding: 8bit 6, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p94E2fTQ005851 ==============================End of - Headers==============================
Hello all, Who has a good source (pharmaceuticals? Jackson immuno-research? etc?) for an anti-yfp Aby? We want to try postembed LR White immunogold label of a yfp expression in drosophila. Pitfalls???
sincerely Michael Delannoy
==============================Original Headers============================== 2, 29 -- From prvs=251ddf6a4=delannoy-at-jhmi.edu Tue Oct 4 13:22:16 2011 2, 29 -- Received: from ipex3.johnshopkins.edu (ipex3.johnshopkins.edu [128.220.161.140]) 2, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p94IMFj7028088 2, 29 -- for {microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 13:22:15 -0500 2, 29 -- X-IronPort-AV: E=Sophos;i="4.68,486,1312171200"; 2, 29 -- d="scan'208";a="148368758" 2, 29 -- Received: from jhem01.johnshopkins.edu (HELO commsmail.johnshopkins.edu) ([10.173.7.11]) 2, 29 -- by ipex3.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 04 Oct 2011 14:22:15 -0400 2, 29 -- MIME-version: 1.0 2, 29 -- Content-transfer-encoding: 7BIT 2, 29 -- Content-disposition: inline 2, 29 -- Content-type: text/plain; CHARSET=US-ASCII 2, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 2, 29 -- by commsmail.johnshopkins.edu 2, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 2, 29 -- with ESMTP id {0LSK00M280D39UF0-at-commsmail.johnshopkins.edu} for 2, 29 -- microscopy-at-microscopy.com; Tue, 04 Oct 2011 14:22:15 -0400 (EDT) 2, 29 -- Received: from [10.173.109.13] (Forwarded-For: [10.16.66.62]) 2, 29 -- by commsmail.johnshopkins.edu (mshttpd); Tue, 04 Oct 2011 14:22:15 -0400 2, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 2, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 29 -- Message-id: {7200ca37184f0.4e8b1697-at-johnshopkins.edu} 2, 29 -- Date: Tue, 04 Oct 2011 14:22:15 -0400 2, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 2, 29 -- 2009) 2, 29 -- Content-language: en 2, 29 -- Subject: anti yfp 2, 29 -- X-Accept-Language: en 2, 29 -- Priority: normal ==============================End of - Headers==============================
I am forwarding this request for a colleague that does not subscribe to the list. I will collect responses and forward them on.
He has a Spot Insight camera model 3.2.0 (about 10 years old) attached to a light microscope. It is currently running off of a PCI card in a Windows XP box. His IT folks are trying to move him over to a new box running Windows 7 and the process is not going well.
When they install the interface card in the new HP computer, it fails to boot up. The DVD light flashes a bit, but the disk drive light never comes on. I am wondering if there is a resource or power problem.
There will then come the issue of drivers and finding a way for the old driver to work on Windows 7.
Has anyone successfully migrated this camera over to Windows 7 that you could share your insight? Is there any support for this available from the company these days? My friend tells me his messages have gone unreplied.
Thanks in advance. Warren Straszheim
==============================Original Headers============================== 7, 28 -- From wesaia-at-iastate.edu Tue Oct 4 15:42:39 2011 7, 28 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p94Kgcbp014192 7, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 4 Oct 2011 15:42:39 -0500 7, 28 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702d.its.iastate.edu [10.10.87.141]) 7, 28 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id p94KgcQ0030005; 7, 28 -- Tue, 4 Oct 2011 15:42:38 -0500 7, 28 -- Received: from EXITS711.its.iastate.edu ([10.10.87.143]) by 7, 28 -- EXITS702D.its.iastate.edu ([::1]) with mapi; Tue, 4 Oct 2011 15:42:32 -0500 7, 28 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 7, 28 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 28 -- Date: Tue, 4 Oct 2011 15:44:53 -0500 7, 28 -- Subject: Using a Spot Insight camera under Windows 7 7, 28 -- Thread-Topic: Using a Spot Insight camera under Windows 7 7, 28 -- Thread-Index: AcyC1nfMpfjVM5NGRs2SP1lpSLaWYQ== 7, 28 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F17790B40FA-at-EXITS711.its.iastate.edu} 7, 28 -- Accept-Language: en-US 7, 28 -- Content-Language: en-US 7, 28 -- X-MS-Has-Attach: 7, 28 -- X-MS-TNEF-Correlator: 7, 28 -- acceptlanguage: en-US 7, 28 -- Content-Type: text/plain; charset="us-ascii" 7, 28 -- MIME-Version: 1.0 7, 28 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.10.4.203015 7, 28 -- X-ISUMailhub-test: Gauge=XIIIIIIIII, Probability=19%, Report=' 7, 28 -- RATWARE_RCVD_BONUS_SPC 2.0, SUPERLONG_LINE 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_1099 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, DATE_TZ_NEG_0500 0, ECARD_WORD 0, FROM_NAME_PHRASE 0, NO_URI_FOUND 0, OEM_SOFTWARE_X1 0, __CP_AGE_BODY 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __INT_PROD_COMP 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __OEM_SOFTWARE_1 0, __SANE_MSGID 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0' 7, 28 -- Content-Transfer-Encoding: 8bit 7, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p94Kgcbp014192 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bfoster-at-mme1.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bfoster-at-mme1.com Name: Barbara Foster
Organization: MME, Inc Title-Subject: [Filtered] Interview with Dr. Shinya Inoue
Message: Hi, Group
For those of you who have attended any of the courses at Woods Hole/Marine Biological Lab, Shinya is a well known and much-beloved figure. His "microscope" is legendary and they show only a glimpse of it here. I came across this recent interview with him and thought you might enjoy it. http://jcb.rupress.org/content/194/6/810 Best regards, Barbara Foster, President and Sr. Consultant
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Tracy. Lawrence-at-inspection.gc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Tracy. Lawrence-at-inspection.gc.ca Name: Tracy Lawrence
Message: I have a 2% fromvar solution that I have used frequently and now has dust etc in it. Rather than have to disposal of through hazardous waste, I would like to filter it and reuse. Does anyine know what kind, size of filter paper I should use? My solution is in cholorform. Thank you, Tracy CFIA Sidney Canada
There are 3rd order Fe Ka1 and Ka2 peaks close to the Au Ma that I presume you are using, The easiest way to check if the Fe is interfering is to run your Fe standard as an unknown and see if you get any Au .
Good Luck Pat
Patrick R Kelly Operations Manager Geotrack International Pty Ltd ABN16 006 821 209 37 Melville Road, Brunswick West, Victoria 3055 Australia Telephone: +613 93801077 Facsimile: +613 93801477 http://www.geotrack.com.au
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com Sent: Wednesday, October 05, 2011 12:00 AM To: probe-at-geotrack.com.au
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yashasin-at-rocketmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: yashasin-at-rocketmail.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Au analyzing
Message: Hi all Analyzing the Magnetite and pyrite with EPMA SX100 shows 1 to 1.5% Au in some points, is it natural or may be wrong? also our condition is: 15Kv, 20 nA and beam size 3um. and in BSE pictures have not been observed any visible gold. How much is the high grade Au concentration in Magnetite and Pyrite? there are some results: Point Fe Co Ni Au Total 1 / 1 . 61.27 0.23 1.05 1.29 66.03 2 / 1 . 60.95 0.42 0.05 0.93 63.57 3 / 1 . 53.95 0 0 1.43 56.4
I got four meaningful replies to my query - ALONG WITH 11 OUT OF OFFICE REPLIES so far. I suppose a few more are still on the way.
I appreciate wanting to maintain contact with customers and clients and not leave them hanging in your absence. However, I am always a little bemused to be notified of who to contact in your absence when I am not your customer or client and really don't care that you are absent.
I concur with the list guidelines and wish ones would somehow manage to exempt list messages from your out-of-office messages.
Now back to the issue- More information, the camera is model 3.2.0 and the software version is 3.2.4. The new computer is a HP Compaq 6200 pro running Windows 7 (32 bit).
The new computer fails to boot at all. I get the impression that the normal diagnostic boot screens fail to appear. The problem occurs in the earliest stages.
I did hear back from the folks at Diagnostic Instruments and Spot Imaging. The problem seems to be that the card requires 5V from the PCI slot and many motherboards fail to provide it. They offer 3.3V instead.
There are plenty of pages out on the net describing the 3.3 vs. 5 V problem. Wikipedia has a nice one. Apparently, a new standard suggested to some manufactures that 5V was to be excluded from the slots conforming to the new specification. That was a debatable reading. It does not have to be excluded. Yet, some manufacturers have dropped it. Dell was mentioned in particular. It may also be the case with HP.
This is probably an issue, or will be, with many instrument interface cards as we move to new computers. I will now know to specifically check to see if the card needs 5V and if the motherboard offers it.
One other reply cited that they were not able to get their Spot card to work with Windows 7-64 bit. Spot imaging says they are working on it. They said their current software and drivers already works with Windows 7-32, and they are working on the 64-bit version. They have a universal voltage interface card that includes an update to the new software.
So it looks like we have some options. We can find a motherboard that supplies 5 V to the PCI slots. Then we will have to go through the exercise of seeing if the XP drivers will work under Windows 7. Or we can go buy a new interface card at what seems a reasonable price and get the new software.
Warren ________________________________________ X-from: Patricia Furgal [mailto:pat.furgal-at-spotimaging.com] Sent: Tuesday, October 04, 2011 4:50 PM To: wesaia-at-iastate.edu Cc: 'David Springett'; 'Stephan Palmer'
The Iowa Microscopy Society (IMS) will hold its annual meeting Friday, November 4th. The talks will be present in room 283 of the Eckstein Medical Research Building on the University of Iowa. The agenda is as follows: Iowa Microscopy Society Fall Meeting 2011 November 4, 2011 Titles and Speakers: Two-Photon Fluorescence Lifetime FRET and single Molecule Imaging in the Study of Prestin" Richard Hallworth, Ph. D. Creighton University
"Tracking Immunity and Tissue Responses In Vivo with Intravital Multiphoton Microscopy" Alex Huang, M.D., Ph. D. The Case Western Reserve University
"Confocal and Multiphoton Approaches to Imaging Glial Cell Responses to Injury in Developing Mouse Brain" Michael Dailey, Ph. D. University of Iowa
"Two-Photon Derived Insights into the Dynamics of Endogenous Cochlear Metabolism" Heather Smith, Ph. D. Creighton University
"Exploring neuronal function with cellular and subcellular fluorescent microscopy in real time: Ca2+ signaling and beyond" Yuriy Usachev, Ph. D. University of Iowa
"Anatomic and Physiologic Small Animal Imaging: An Expanding Palette" John Sunderland, Ph. D. University of Iowa
"Fluorescent Probes for Live Cell and Whole Animal imaging" Michael Ignatius Life Technologies
Pre-registration is not required, attendance is free (membership in IMS or MSA highly encouraged). Lunch will be provided for IMS members. Watch the IMS website for updates and talk time schedules as the program is solidified: http://www.uiowa.edu/~cmrf/ims/news/news.html
________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________
==============================Original Headers============================== 13, 27 -- From randy-nessler-at-uiowa.edu Wed Oct 5 10:45:23 2011 13, 27 -- Received: from HC-EDGE2.healthcare.uiowa.edu (hc-edge2.healthcare.uiowa.edu [129.255.126.23]) 13, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p95FjNwV016836 13, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Oct 2011 10:45:23 -0500 13, 27 -- Received: from HC-HUB1.healthcare.uiowa.edu (129.255.112.194) by 13, 27 -- HC-EDGE2.healthcare.uiowa.edu (129.255.126.23) with Microsoft SMTP Server 13, 27 -- (TLS) id 14.1.289.1; Wed, 5 Oct 2011 10:45:21 -0500 13, 27 -- Received: from HC-MAILBOXC1-N5.healthcare.uiowa.edu ([169.254.5.147]) by 13, 27 -- HC-HUB1.healthcare.uiowa.edu ([129.255.112.194]) with mapi id 14.01.0289.001; 13, 27 -- Wed, 5 Oct 2011 10:45:23 -0500 13, 27 -- From: "Nessler, Randy A" {randy-nessler-at-uiowa.edu} 13, 27 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 13, 27 -- Subject: Iowa Microscopy Society Meeting 13, 27 -- Thread-Topic: Iowa Microscopy Society Meeting 13, 27 -- Thread-Index: AcyDda/OmjWi5ZC1QFyige0uXO4R8w== 13, 27 -- Date: Wed, 5 Oct 2011 15:45:22 +0000 13, 27 -- Message-ID: {962DA0541BCBF346AAC391AE682C87A20C82E2EB-at-HC-MAILBOXC1-N5.healthcare.uiowa.edu} 13, 27 -- Accept-Language: en-US 13, 27 -- Content-Language: en-US 13, 27 -- X-MS-Has-Attach: 13, 27 -- X-MS-TNEF-Correlator: 13, 27 -- x-originating-ip: [172.26.0.1] 13, 27 -- x-my-disclaimer: ready 13, 27 -- Content-Type: text/plain; charset="us-ascii" 13, 27 -- MIME-Version: 1.0 13, 27 -- Content-Transfer-Encoding: 8bit 13, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p95FjNwV016836 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nesmicroscopy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: The New England Society for Microscopy
Title-Subject: [Filtered] NESM Fall Meeting Oct. 20th: Almost Here!
Message: Hello Microscopy Listserverites,
The New England Society for Microscopy will be holding their annual Fall Meeting -at- Harvard University in just a few short weeks (October 20th). This is a spectacular chance to attend informative workshops (BioSEM, BioTEM, CARS, Super Resolution SI/PAL-M, and X-ray MicroCT) followed by a dinner meeting:
"Imaging the Connectome", Jeff Lichtman, Ph.D., Harvard University
"Super Resolution Light Microscopy: Structured Illumination and PAL-M", Bernhard Goetze, Ph.D., Zeiss
Workshop space is limited. Register Today!
Please visit our new website for information: http://nesmicroscopy.org/
An opportunity exists for an Electron Microscopy Technician to work in the Otago Centre for Electron Microscopy at the University of Otago. The appointee will principally support scanning electron microscopy, and be responsible for developing correlative light and transmission electron microscopy techniques for use by researchers.
The ideal applicant will have an enthusiasm for electron microscopy with experience in either scanning electron microscopy and/or transmission electron microscopy. Previous experience in immunofluorescence and/or immunocytochemistry is highly desirable. The appointee will be expected to successfully train users of the Centre in these techniques, and keep up-to-date with developments in these areas. The ability to work as part of a team, cope with a wide range of demands, work without direct supervision, set priorities, and bring enthusiasm to the job is essential.
Specific enquiries may be directed to Mr Allan Mitchell, Manager of the Otago Centre for Electron Microscopy.
For more information and a copy of the job description go to the University of Otago web site and look under current vacancies.
Applications quoting reference number 1100517 will close on Friday, 28 October 2011.
Allan Mitchell Microscopy Otago - Electron Microscopy c/- Department of Anatomy Otago School of Medical Sciences P.O. Box 913 Dunedin New Zealand
==============================Original Headers============================== 5, 20 -- From allan.mitchell-at-stonebow.otago.ac.nz Wed Oct 5 23:39:48 2011 5, 20 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p964dliV028927 5, 20 -- for {microscopy-at-microscopy.com} ; Wed, 5 Oct 2011 23:39:47 -0500 5, 20 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 5, 20 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id p964dkwZ013589 5, 20 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 17:39:46 +1300 5, 20 -- Received: from ou040139.otago.ac.nz (ou040139.otago.ac.nz [139.80.40.139]) 5, 20 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id 5F0834F7B929 5, 20 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 17:39:46 +1300 (NZDT) 5, 20 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 5, 20 -- Content-Type: text/plain; charset=us-ascii 5, 20 -- Subject: Electron Microscopy Technician position available 5, 20 -- Date: Thu, 6 Oct 2011 17:39:46 +1300 5, 20 -- Message-Id: {139D6D04-018D-4738-ACC1-EE1E4E0A0F83-at-stonebow.otago.ac.nz} 5, 20 -- To: microscopy-at-microscopy.com 5, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 5, 20 -- X-Mailer: Apple Mail (2.1084) 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p964dliV028927 ==============================End of - Headers==============================
The Midwest Microscopy and Microanalysis Society will hold its final meeting of 2011 on Friday, November 4th, at Baxter Healthcare in Deerfield, IL. The program is titled, "Spectroscopy Techniques for Materials and Biological Microscopy". Program details and registration information can be found on our website under Meetings:
www.midwestmicroscopy.org
Ke-Bin Low and Robert Klie have invited an excellent group of speakers. We look forward to seeing you there!
Elaine Schumacher M3S Program Coordinator
********************************************************************* Elaine F.Schumacher Senior Research Scientist McCrone Associates, Inc. 850 Pasquinelli Drive Westmont, IL 60559-5539 USA 630-887-7100 (tel) 630-887-7417 (fax) E-mail: eschumacher-at-mccrone.com Web Site: www.mccrone.com
********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited. *********************************************************************
==============================Original Headers============================== 11, 27 -- From eschumacher-at-mccrone.com Thu Oct 6 07:38:19 2011 11, 27 -- Received: from mail.mccrone.com (mail.mccrone.com [12.54.22.114]) 11, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p96CcJ0J019565 11, 27 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 07:38:19 -0500 11, 27 -- Received: from mccronemsg07.tmg.mccrone.com ([::1]) by 11, 27 -- mccronemsg07.tmg.mccrone.com ([::1]) with mapi; Thu, 6 Oct 2011 07:38:18 11, 27 -- -0500 11, 27 -- From: "Elaine F. Schumacher" {eschumacher-at-mccrone.com} 11, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 27 -- Date: Thu, 6 Oct 2011 07:38:16 -0500 11, 27 -- Subject: Meeting Announcement: Midwest Microscopy and Microanalysis Society 11, 27 -- Thread-Topic: Meeting Announcement: Midwest Microscopy and Microanalysis 11, 27 -- Society 11, 27 -- Thread-Index: AcyEJNIUhppv/a6VShuO8V7v14HRZQ== 11, 27 -- Message-ID: {874B1DB532886444A60A015EE363493F5E1F139D62-at-mccronemsg07.tmg.mccrone.com} 11, 27 -- Accept-Language: en-US 11, 27 -- Content-Language: en-US 11, 27 -- X-MS-Has-Attach: 11, 27 -- X-MS-TNEF-Correlator: 11, 27 -- x-vipre-scanned: 0F9E35AF0029B40F9E36FC 11, 27 -- x-ninja-pim: Scanned by Ninja 11, 27 -- x-ninja-attachmentfiltering: (no action) 11, 27 -- acceptlanguage: en-US 11, 27 -- Content-Type: text/plain; charset="us-ascii" 11, 27 -- MIME-Version: 1.0 11, 27 -- Content-Transfer-Encoding: 8bit 11, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p96CcJ0J019565 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckimmye-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mckimmye-at-gmail.com Name: Emily McKimmy
Organization: TRW Automotive
Title-Subject: [Filtered] EDS resolution issue
Message: I am having an issue with our EDS that if we have carbon tape next to copper tape and do Point and Shoot or mapping mode no matter where we are on the copper tape we see some carbon and no matter where we are on the carbon tape we see some copper. If I put in a stub with only copper, I see only copper and the same is true for carbon, so I am not getting stray X-rays from the surroundings (column etc). When I put in a sample with two different types of tape, I always see a little of the other element. My concern is when doing an unknown sample that is a failure I will not be able to target the area of concern, I will see the surrounding area as well. We had a Noran System Six EDS on our ElectroScan (now FEI) 2020 Environmental SEM, I noticed this problem. I called the EDS service engineer and he found that we were missing the collimator on the EDS. There was no replacement collimator and we got a new Noran System Seven Ultra Dry SDD. We are still having the issue. The EDS engineer feels the SEM beam is not focused/ spreading and that is the issue. I am also unsure if it is the vacuum levels. We use typically use 2 Torr and using 0.1 Torr was recommended by an EDS applications person (not for this issue but in general), but the vacuum won’t go below 0.3 Torr and imaging at the lower vacuum is troublesome. Is it realistic to expect to see only copper when on copper tape with carbon tape 100um away? Is there a way I determine the beam ‘footprint’ size somehow? Thank-you.
My first guess would be that you are getting beam spreading due to the residual gas in your ESEM. Try a scan on a good conductive sample using high-vacuum. If your system is operating correctly you should pick up no stray X-ray signal. The tails due to the scattering by the gas can extend pretty far away from the primary beam. The scattering is a function of the gas pressure and the path length through the gas, so the longer the working distance, the worse the beam tails will be.
You can test the beam spreading by using a carbon planchet with a Cu grid or Cu tape on it. Start with the beam far away from the Cu and take spectra as you move the beam toward the Cu. Try this in a good hard vacuum and then with various gas pressures in the chamber. If your detector geometry allows, try different working distances too.
Interestingly, the tails generally do not have a strong effect on the imaging resolution. You will see a significant contrast drop before the resolution is greatly affected.
Scott Wight's article may be useful. Scott A. Wight, Experimental data and model simulations of beam spread in the environmental scanning electron microscope. Scanning, v23, {http://onlinelibrary.wiley.com/doi/10.1002/sca.v23:5/issuetoc} pages 320–327 , September/October 2001
Cheers, Henk
At 10/6/2011 9:11 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mckimmye-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mckimmye-at-gmail.com Name: Emily McKimmy } } Organization: TRW Automotive } } Title-Subject: [Filtered] EDS resolution issue } } Message: I am having an issue with our EDS that if we have carbon tape next to copper tape and do } Point and Shoot or mapping mode no matter where we are on the copper tape we see some carbon and no } matter where we are on the carbon tape we see some copper. If I put in a stub with only copper, I } see only copper and the same is true for carbon, so I am not getting stray X-rays from the } surroundings (column etc). When I put in a sample with two different types of tape, I always see a } little of the other element. My concern is when doing an unknown sample that is a failure I will } not be able to target the area of concern, I will see the surrounding area as well. We had a Noran } System Six EDS on our ElectroScan (now FEI) 2020 Environmental SEM, I noticed this problem. I } called the EDS service engineer and he found that we were missing the collimator on the EDS. There } was no replacement collimator and we got a new Noran System Seven Ultra Dry SDD. We are still } having the issue. The EDS engineer feels the SEM beam is not focused/ spreading and that is the } issue. I am also unsure if it is the vacuum levels. We use typically use 2 Torr and using 0.1 Torr } was recommended by an EDS applications person (not for this issue but in general), but the vacuum } won’t go below 0.3 Torr and imaging at the lower vacuum is troublesome. Is it realistic to expect } to see only copper when on copper tape with carbon tape 100um away? Is there a way I determine the } beam ‘footprint’ size somehow? Thank-you. } } Login Host: 12.173.204.42 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Oct 6 20:10:11 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p971AB45016477 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:11 -0500 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 2ED35421BFD } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:11 -0500 (CDT) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id B3s106zx+dQ3 for {microscopy-at-microscopy.com} ; } 8, 25 -- Thu, 6 Oct 2011 20:10:08 -0500 (CDT) } 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 3E4B7421BF2 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:08 -0500 (CDT) } 8, 25 -- Message-ID: {4E8E516F.2070406-at-microscopy.com} } 8, 25 -- Date: Thu, 06 Oct 2011 20:10:07 -0500 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: mckimmye-at-gmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:EDS "spatial" resolution issue } 8, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 8, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 10, 27 -- From colijn.1-at-osu.edu Thu Oct 6 20:53:30 2011 10, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p971rU7D000988 10, 27 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:53:30 -0500 10, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 27 -- id {01O6WUP6W0LS92SROO-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 10, 27 -- Thu, 06 Oct 2011 21:53:26 -0400 (EDT) 10, 27 -- Received: from [164.107.97.86] 10, 27 -- (vpn-164-107-97-86.vpn.ohio-state.edu [164.107.97.86]) 10, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 27 -- with ESMTPA id {01O6WUOZH8FE946BA9-at-ecr6.ohio-state.edu} ; Thu, 10, 27 -- 06 Oct 2011 21:53:16 -0400 (EDT) 10, 27 -- Date: Thu, 06 Oct 2011 21:53:15 -0400 10, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 27 -- Subject: Re: [Microscopy] viaWWW:EDS "spatial" resolution issue 10, 27 -- In-reply-to: {201110070111.p971BkYZ018337-at-ns.microscopy.com} 10, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 27 -- To: microscopy-at-microscopy.com, mckimmye-at-gmail.com 10, 27 -- Message-id: {4E8E5B8B.2090508-at-osu.edu} 10, 27 -- MIME-version: 1.0 10, 27 -- Content-type: text/plain; charset=windows-1252; format=flowed 10, 27 -- Content-transfer-encoding: 8BIT 10, 27 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 10, 27 -- Thunderbird/7.0.1 10, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 27 -- References: {201110070111.p971BkYZ018337-at-ns.microscopy.com} ==============================End of - Headers==============================
I agree with Henk's assessment below. Many variable pressure instruments have X-ray cones that fit on the bottom of the final lens below the vacuum-limiting aperture. These protect the beam from the low vacuum interaction for a greater distance and decrease the spread. See if this is available for your instrument as it may solve your problem to a significant extent.
Debby
--- Debby Sherman, Director Phone: 765-494-6666 Life Science Microscopy Facility FAX: 765-494-5896 Purdue University E-mail: dsherman-at-purdue.edu S-052 Whistler Building 170 S. University Street West Lafayette, IN 47907 http://www.ag.purdue.edu/facilities/microscopy
On 10/6/11 9:54 PM, "Hendrik Colijn" {colijn.1-at-OSU.edu} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Emily, } } My first guess would be that you are getting beam spreading due to the } residual gas in your ESEM. Try a scan on a good conductive sample using } high-vacuum. If your system is operating correctly you should pick up no } stray X-ray signal. The tails due to the scattering by the gas can } extend pretty far away from the primary beam. The scattering is a } function of the gas pressure and the path length through the gas, so the } longer the working distance, the worse the beam tails will be. } } You can test the beam spreading by using a carbon planchet with a Cu } grid or Cu tape on it. Start with the beam far away from the Cu and take } spectra as you move the beam toward the Cu. Try this in a good hard } vacuum and then with various gas pressures in the chamber. If your } detector geometry allows, try different working distances too. } } Interestingly, the tails generally do not have a strong effect on the } imaging resolution. You will see a significant contrast drop before the } resolution is greatly affected. } } Scott Wight's article may be useful. } Scott A. Wight, Experimental data and model simulations of beam spread } in the environmental scanning electron microscope. } Scanning, v23, } {http://onlinelibrary.wiley.com/doi/10.1002/sca.v23:5/issuetoc} pages } 320327 , September/October 2001 } } Cheers, } Henk } } } At 10/6/2011 9:11 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when replying } } please copy both } } mckimmye-at-gmail.com as well as the MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: mckimmye-at-gmail.com Name: Emily McKimmy } } } } Organization: TRW Automotive } } } } Title-Subject: [Filtered] EDS resolution issue } } } } Message: I am having an issue with our EDS that if we have carbon tape next } } to copper tape and do } } Point and Shoot or mapping mode no matter where we are on the copper tape we } } see some carbon and no } } matter where we are on the carbon tape we see some copper. If I put in a } } stub with only copper, I } } see only copper and the same is true for carbon, so I am not getting stray } } X-rays from the } } surroundings (column etc). When I put in a sample with two different types } } of tape, I always see a } } little of the other element. My concern is when doing an unknown sample that } } is a failure I will } } not be able to target the area of concern, I will see the surrounding area as } } well. We had a Noran } } System Six EDS on our ElectroScan (now FEI) 2020 Environmental SEM, I } } noticed this problem. I } } called the EDS service engineer and he found that we were missing the } } collimator on the EDS. There } } was no replacement collimator and we got a new Noran System Seven Ultra Dry } } SDD. We are still } } having the issue. The EDS engineer feels the SEM beam is not focused/ } } spreading and that is the } } issue. I am also unsure if it is the vacuum levels. We use typically use 2 } } Torr and using 0.1 Torr } } was recommended by an EDS applications person (not for this issue but in } } general), but the vacuum } } won¹t go below 0.3 Torr and imaging at the lower vacuum is troublesome. Is } } it realistic to expect } } to see only copper when on copper tape with carbon tape 100um away? Is there } } a way I determine the } } beam Œfootprint¹ size somehow? Thank-you. } } } } Login Host: 12.173.204.42 } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Oct 6 20:10:11 } } 2011 } } 8, 25 -- Received: from znl.com ([206.69.208.20]) } } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } } p971AB45016477 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:11 -0500 } } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } } 8, 25 -- by znl.com (Postfix) with ESMTP id 2ED35421BFD } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:11 -0500 } } (CDT) } } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 8, 25 -- Received: from znl.com ([127.0.0.1]) } } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } } 10024) } } 8, 25 -- with ESMTP id B3s106zx+dQ3 for {microscopy-at-microscopy.com} ; } } 8, 25 -- Thu, 6 Oct 2011 20:10:08 -0500 (CDT) } } 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } } 8, 25 -- by znl.com (Postfix) with ESMTPA id 3E4B7421BF2 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:08 -0500 } } (CDT) } } 8, 25 -- Message-ID: {4E8E516F.2070406-at-microscopy.com} } } 8, 25 -- Date: Thu, 06 Oct 2011 20:10:07 -0500 } } 8, 25 -- From: } } MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } } 8, 25 -- Reply-To: mckimmye-at-gmail.com } } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 8, 25 -- MIME-Version: 1.0 } } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 8, 25 -- Subject: viaWWW:EDS "spatial" resolution issue } } 8, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } } 8, 25 -- Content-Transfer-Encoding: 8bit } } ==============================End of - Headers============================== } }
==============================Original Headers============================== 10, 29 -- From dsherman-at-purdue.edu Thu Oct 6 21:40:29 2011 10, 29 -- Received: from mailhub130.itcs.purdue.edu (mailhub130.itcs.purdue.edu [128.210.5.130]) 10, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p972eTYA017953 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 21:40:29 -0500 10, 29 -- Received: from WPPEXHUB04H.purdue.lcl (wppexhub04h.itap.purdue.edu [172.21.6.93]) 10, 29 -- by mailhub130.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id p972eSWg002213 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 22:40:28 -0400 10, 29 -- Received: from VPEXCH04.purdue.lcl ([169.254.2.167]) by WPPEXHUB04H.purdue.lcl 10, 29 -- ([::1]) with mapi; Thu, 6 Oct 2011 22:40:28 -0400 10, 29 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu} 10, 29 -- To: "message to: MSA list" {microscopy-at-microscopy.com} 10, 29 -- Date: Thu, 6 Oct 2011 22:40:25 -0400 10, 29 -- Subject: Re: [Microscopy] Re: viaWWW:EDS "spatial" resolution issue 10, 29 -- Thread-Topic: [Microscopy] Re: viaWWW:EDS "spatial" resolution issue 10, 29 -- Thread-Index: AcyElBIqZkbVx8JITF2MmJeeRS807gABmU9U 10, 29 -- Message-ID: {CAB3DED9.12656%dsherman-at-purdue.edu} 10, 29 -- In-Reply-To: {201110070154.p971sakN003686-at-ns.microscopy.com} 10, 29 -- Accept-Language: en-US 10, 29 -- Content-Language: en-US 10, 29 -- X-MS-Has-Attach: 10, 29 -- X-MS-TNEF-Correlator: 10, 29 -- user-agent: Microsoft-Entourage/13.11.0.110726 10, 29 -- acceptlanguage: en-US 10, 29 -- Content-Type: text/plain; charset="Windows-1252" 10, 29 -- MIME-Version: 1.0 10, 29 -- X-PMX-Version: 5.5.9.388399 10, 29 -- X-PerlMx-Virus-Scanned: Yes 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p972eTYA017953 ==============================End of - Headers==============================
The problem you report is a classic problem with performing EDS analysis in an environmental SEM. In an environmental SEM, the images can look crisp and high resolution even at relatively high pressures (you report 2 Torr). However, the images are deceptive. The image contrast is primarily formed by the unscattered central node of the electron beam. However, because of the gas in the chamber a large fraction of the electrons in the beam are scattered once or more times before interacting with the sample. A single scattering event can deflect a beam electron by hundreds of microns. The deflected electrons cause the central node of the beam to be surrounded by a skirt which may be hundreds of microns in diameter. Depending on the gas path length and the gas pressure in the chamber you may only have a most of the beam electrons in the unscattered central node or you may have a very small fraction. It sounds like you might be in a situation in which a large fraction of the electrons are being scattered into the skirt. The skirt makes quantitative x-ray microanalysis much more difficult. A handful of techniques have been proposed for dealing with the skirt but the simplest is to reduce the effective gas path length until the skirt is no longer a problem.
You can use the Monte Carlo simulator built into NIST DTSA-II (http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/index.html) to model your environmental SEM. The simulations can provide insight into the magnitude of the problem and how you might resolve it.
Nicholas
======================================= Nicholas W. M. Ritchie Physicist, Surface and Microanalysis Science Division National Institute of Standards and Technology 100 Bureau Drive, MS: 8371 Gaithersburg, MD 20899-8371 (Work) (301) 975-3929 (Cell) (240) 883-8982
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, October 06, 2011 9:14 PM To: Ritchie, Nicholas
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckimmye-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mckimmye-at-gmail.com Name: Emily McKimmy
Organization: TRW Automotive
Title-Subject: [Filtered] EDS resolution issue
Message: I am having an issue with our EDS that if we have carbon tape next to copper tape and do Point and Shoot or mapping mode no matter where we are on the copper tape we see some carbon and no matter where we are on the carbon tape we see some copper. If I put in a stub with only copper, I see only copper and the same is true for carbon, so I am not getting stray X-rays from the surroundings (column etc). When I put in a sample with two different types of tape, I always see a little of the other element. My concern is when doing an unknown sample that is a failure I will not be able to target the area of concern, I will see the surrounding area as well. We had a Noran System Six EDS on our ElectroScan (now FEI) 2020 Environmental SEM, I noticed this problem. I called the EDS service engineer and he found that we were missing the collimator on the EDS. There was no replacement collimator and we got a new Noran System Seven Ultra Dry SDD. We are still having the issue. The EDS engineer feels the SEM beam is not focused/ spreading and that is the issue. I am also unsure if it is the vacuum levels. We use typically use 2 Torr and using 0.1 Torr was recommended by an EDS applications person (not for this issue but in general), but the vacuum won’t go below 0.3 Torr and imaging at the lower vacuum is troublesome. Is it realistic to expect to see only copper when on copper tape with carbon tape 100um away? Is there a way I determine the beam ‘footprint’ size somehow? Thank-you.
Fully agreeing with your considerations, I noticed Emily mentioning that SEM will not pump below 0.3 Torr. Unless there is an operator mistake somewhere, to me it sounds like her tool is in dire need of repairs...
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/7/2011 9:04 AM, nicholas.ritchie-at-nist.gov wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The problem you report is a classic problem with performing EDS analysis in an environmental SEM. In an environmental SEM, the images can look crisp and high resolution even at relatively high pressures (you report 2 Torr). However, the images are deceptive. The image contrast is primarily formed by the unscattered central node of the electron beam. However, because of the gas in the chamber a large fraction of the electrons in the beam are scattered once or more times before interacting with the sample. A single scattering event can deflect a beam electron by hundreds of microns. The deflected electrons cause the central node of the beam to be surrounded by a skirt which may be hundreds of microns in diameter. Depending on the gas path length and the gas pressure in the chamber you may only have a most of the beam electrons in the unscattered central node or you may have a very small fraction. It sounds like you might be in a situation in which a large fraction of! } the electrons are being scattered into the skirt. The skirt makes quantitative x-ray microanalysis much more difficult. A handful of techniques have been proposed for dealing with the skirt but the simplest is to reduce the effective gas path length until the skirt is no longer a problem. } } You can use the Monte Carlo simulator built into NIST DTSA-II (http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/index.html) to model your environmental SEM. The simulations can provide insight into the magnitude of the problem and how you might resolve it. } } Nicholas } } } ======================================= } Nicholas W. M. Ritchie } Physicist, Surface and Microanalysis Science Division } National Institute of Standards and Technology } 100 Bureau Drive, MS: 8371 } Gaithersburg, MD 20899-8371 } (Work) (301) 975-3929 (Cell) (240) 883-8982 } } -----Original Message----- } X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Thursday, October 06, 2011 9:14 PM } To: Ritchie, Nicholas } Subject: [Microscopy] viaWWW:EDS "spatial" resolution issue } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mckimmye-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mckimmye-at-gmail.com Name: Emily McKimmy } } Organization: TRW Automotive } } Title-Subject: [Filtered] EDS resolution issue } } Message: I am having an issue with our EDS that if we have carbon tape next to copper tape and do Point and Shoot or mapping mode no matter where we are on the copper tape we see some carbon and no matter where we are on the carbon tape we see some copper. If I put in a stub with only copper, I see only copper and the same is true for carbon, so I am not getting stray X-rays from the surroundings (column etc). When I put in a sample with two different types of tape, I always see a little of the other element. My concern is when doing an unknown sample that is a failure I will not be able to target the area of concern, I will see the surrounding area as well. We had a Noran System Six EDS on our ElectroScan (now FEI) 2020 Environmental SEM, I noticed this problem. I called the EDS service engineer and he found that we were missing the collimator on the EDS. There was no replacement collimator and we got a new Noran System Seven Ultra Dry SDD. We are still having the i! } ssue. The EDS engineer feels the SEM beam is not focused/ spreading and that is the issue. I am also unsure if it is the vacuum levels. We use typically use 2 Torr and using 0.1 Torr was recommended by an EDS applications person (not for this issue but in general), but the vacuum won’t go below 0.3 Torr and imaging at the lower vacuum is troublesome. Is it realistic to expect to see only copper when on copper tape with carbon tape 100um away? Is there a way I determine the beam ‘footprint’ size somehow? Thank-you. } } Login Host: 12.173.204.42 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Oct 6 20:10:11 2011 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p971AB45016477 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:11 -0500 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 2ED35421BFD } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:11 -0500 (CDT) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id B3s106zx+dQ3 for {microscopy-at-microscopy.com} ; } 8, 25 -- Thu, 6 Oct 2011 20:10:08 -0500 (CDT) } 8, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 3E4B7421BF2 } 8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 6 Oct 2011 20:10:08 -0500 (CDT) } 8, 25 -- Message-ID: {4E8E516F.2070406-at-microscopy.com} 8, 25 -- Date: Thu, 06 Oct 2011 20:10:07 -0500 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: mckimmye-at-gmail.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 8, 25 -- MIME-Version: 1.0 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 8, 25 -- Subject: viaWWW:EDS "spatial" resolution issue 8, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed 8, 25 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 18, 29 -- From nicholas.ritchie-at-nist.gov Fri Oct 7 08:02:44 2011 } 18, 29 -- Received: from wsget2.nist.gov (wsget2.nist.gov [129.6.13.151]) } 18, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p97D2ieR015960 } 18, 29 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 08:02:44 -0500 } 18, 29 -- Received: from WSXGHUB1.xchange.nist.gov (129.6.18.96) by wsget2.nist.gov } 18, 29 -- (129.6.13.151) with Microsoft SMTP Server (TLS) id 14.1.323.3; Fri, 7 Oct } 18, 29 -- 2011 09:02:35 -0400 } 18, 29 -- Received: from MBCLUSTER.xchange.nist.gov ([fe80::41df:f63f:c718:e08]) by } 18, 29 -- WSXGHUB1.xchange.nist.gov ([129.6.18.96]) with mapi; Fri, 7 Oct 2011 09:02:43 } 18, 29 -- -0400 } 18, 29 -- From: "Ritchie, Nicholas" {nicholas.ritchie-at-nist.gov} } 18, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 18, 29 -- CC: "mckimmye-at-gmail.com" {mckimmye-at-gmail.com} } 18, 29 -- Date: Fri, 7 Oct 2011 09:02:42 -0400 } 18, 29 -- Subject: RE: [Microscopy] viaWWW:EDS "spatial" resolution issue } 18, 29 -- Thread-Topic: [Microscopy] viaWWW:EDS "spatial" resolution issue } 18, 29 -- Thread-Index: AcyEjmIYD76DlRsOQlSe+A9snU1aXQAX7M6Q } 18, 29 -- Message-ID: {7E80450DE7474E4881E405C742AC0DEA1256C416CD-at-MBCLUSTER.xchange.nist.gov} } 18, 29 -- References: {201110070114.p971ERUC020201-at-ns.microscopy.com} } 18, 29 -- In-Reply-To: {201110070114.p971ERUC020201-at-ns.microscopy.com} } 18, 29 -- Accept-Language: en-US } 18, 29 -- Content-Language: en-US } 18, 29 -- X-MS-Has-Attach: } 18, 29 -- X-MS-TNEF-Correlator: } 18, 29 -- acceptlanguage: en-US } 18, 29 -- Content-Type: text/plain; charset="utf-8" } 18, 29 -- MIME-Version: 1.0 } 18, 29 -- Content-Transfer-Encoding: 8bit } 18, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id p97D2ieR015960 } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 44 -- From vray-at-partbeamsystech.com Fri Oct 7 11:14:21 2011 4, 44 -- Received: from nm30.bullet.mail.sp2.yahoo.com (nm30.bullet.mail.sp2.yahoo.com [98.139.91.100]) 4, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p97GEKGd004011 4, 44 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 11:14:20 -0500 4, 44 -- Received: from [98.139.91.62] by nm30.bullet.mail.sp2.yahoo.com with NNFMP; 07 Oct 2011 16:14:20 -0000 4, 44 -- Received: from [98.139.91.11] by tm2.bullet.mail.sp2.yahoo.com with NNFMP; 07 Oct 2011 16:13:27 -0000 4, 44 -- Received: from [127.0.0.1] by omp1011.mail.sp2.yahoo.com with NNFMP; 07 Oct 2011 16:13:27 -0000 4, 44 -- X-Yahoo-Newman-Id: 422981.6134.bm-at-omp1011.mail.sp2.yahoo.com 4, 44 -- Received: (qmail 30215 invoked from network); 7 Oct 2011 16:13:27 -0000 4, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1318004007; bh=2n97eQzbMFSfC8KjiHfuVPKRAhQKipWbwwzFayq/H1U=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=SnABlrfnWJEH2XSVsgCT3OqQYnABizq+AWGHcGI0MKcd8F0hM653XdsTk9ZuBkYm54PZbsTfp183drRk5UP1Xh86/oc5iyosNwPUdgZg30iCdKAdyYlLzd/1eSbdS3gg8pvnYdZ9I1suNVRePh3ckXSYnLCngQbBAL0Sv8bzCWU= 4, 44 -- X-Yahoo-Newman-Property: ymail-3 4, 44 -- X-YMail-OSG: QoOSZeIVM1kz7RTl3MfwW9IXWFG_fSOHHwx0vYhnBX.BURs 4, 44 -- j40bT_189k_cEijcXtgZjfGY4MEks.Z2Nl9qd0lhx7xURJTy1G2xww18G8vP 4, 44 -- O4LTCjQod2FQVIIf._YSs0V57wC8FCctHAq15yR7RPD3BAAHZC3iUag32DoR 4, 44 -- 69G33yxyWUi7DsnlgjNLsXIfqinwX5qL_n8r8tmRxv.dKn8RtVhLWhDOe7ZU 4, 44 -- OK0ZVoIriLch_NZ5uL71Vq9iSn0HwKp7wf8Retx85aKNtZ7mYTGDEPkQYii8 4, 44 -- 88M.texcy16UTg9_Xq7LYfPsCcOMsaR4TLqzCUA1GckibEZeKfX86_XBh3qO 4, 44 -- Sn229BsJAvP0afrR8ce9lRfn1tl1ID8jgI1J3EvrNalBLA_sDjf9xW6H70MQ 4, 44 -- tyKP7zWTQJLXDhndOPo7K1OF.biHqGKUHZHPgrH46nQsQgN6.ANaKY3DvdWx 4, 44 -- 0fgc3w3uX0CI9w6vahw809Pvgs8DHXIvZ0ad0yjE9.PWsjlWJpELnduV.cqC 4, 44 -- IwJabB5HYeIu4Lemms6wykRs4DdFA9V7_Gjl3JpNKBH4e.xx8Z_XnEbAWV0M 4, 44 -- Iav_gt38y5tdPwaZxE4en7ARRJnJCk8yGpJgN.Kid_1BrU_i_ciuTikQrr00 4, 44 -- mf9SIwd6iH31Yh7DbtMFzfrb4xtGbknrQMNGbALbsaof.vhO37FiEu4JDyd3 4, 44 -- bNJ_In50PpWldy6uc0F2SGPFuCnlbSsECX1TqQE2yeH7cA7Ax_PZXcgQoi1X 4, 44 -- jsEg8RgZun2IzFDYsy6zpUmeSlFkWEJz48r9S5DSZNW9VCqwYPdmr7QdhQFV 4, 44 -- oOx0S2rYUA9auic56jzM10e9Ty7agKWBPbsYERn3ApfI1xJWNjZ0kRYFHPS4 4, 44 -- BQVaZVgjyuA-- 4, 44 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 44 -- Received: from [192.168.5.20] (vray-at-64.134.146.240 with plain) 4, 44 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 07 Oct 2011 09:13:26 -0700 PDT 4, 44 -- Message-ID: {4E8F2526.2070306-at-partbeamsystech.com} 4, 44 -- Date: Fri, 07 Oct 2011 12:13:26 -0400 4, 44 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 44 -- Reply-To: vray-at-partbeamsystech.com 4, 44 -- Organization: PBS&T 4, 44 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 4, 44 -- MIME-Version: 1.0 4, 44 -- To: nicholas.ritchie-at-nist.gov 4, 44 -- CC: microscopy-at-microscopy.com 4, 44 -- Subject: Re: [Microscopy] RE: viaWWW:EDS "spatial" resolution issue 4, 44 -- References: {201110071304.p97D45hw017288-at-ns.microscopy.com} 4, 44 -- In-Reply-To: {201110071304.p97D45hw017288-at-ns.microscopy.com} 4, 44 -- Content-Type: text/plain; charset=windows-1252; format=flowed 4, 44 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mckimmye-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Thank-you for quick and extremely helpful answers. The consensus is thatI need to use HV mode for good EDS results. This is the first ESEM I have used and I am going to have to do some reading/ learning to adjust. Login Host: 12.173.204.42 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Oct 7 20:49:22 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p981nMVf028956 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:49:22 -0500 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 4EC85422B33 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:49:22 -0500 (CDT) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id 2OMXqQPDqjyd for {microscopy-at-microscopy.com} ; 7, 25 -- Fri, 7 Oct 2011 20:49:21 -0500 (CDT) 7, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 25 -- by znl.com (Postfix) with ESMTPA id D94CA422B28 7, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:49:21 -0500 (CDT) 7, 25 -- Message-ID: {4E8FAC21.6040106-at-microscopy.com} 7, 25 -- Date: Fri, 07 Oct 2011 20:49:21 -0500 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: mckimmye-at-gmail.com 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:EDS Spatial resolution issue 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibarra27-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibarra27-at-gmail.com Name: Maria Lujan Ibarra
Title-Subject: [Filtered] Preparation of TEM cross sections of Semiconductors
Message: Hi
I need to prepare cross section of SiSiO2 for TEM but the epoxy that is in in the lab is very old. I need to buy an adhesive . Can anyone tell me the name of the epoxy that use for cross section and ion milling? I have an adhesive that we use to space applications: down corning 93500, someone suggest to me that I can use it for TEM. Can anyone tell me if its apropiate for that use?
I use EpoTek-353ND from Epoxy Technology. It has a very long usable shelf life. You can find it online at http://epotek.com/search-results.asp?searchField=353ND
If you are not doing site-specific work, I would also recommend the Small Angle Cleavage Technique, aka MicroCleave(TM) technique for doing your cross sections of semiconducting materials. It's fast, inexpensive, no ion milling, and produces superb samples. See the link to our application note for a description of the technique and how to do it, http://southbaytech.com/appnotes/62%20The%20Small%20Angle%20Cleavage%20Techn ique%20An%20Update.pdf There are a few other application notes related to SACT that we have, but this is the one that tells you how to do it.
In about the time that it takes to glue your samples and cut your samples, you could have about 9 great samples using SACT ready to go into the microscope.
Disclaimer: SBT manufactures and sells the MicroCleave(TM) Kit.
-Scott
Scott D. Walck, Ph.D. Technical Director South Bay Technology, Inc. 1120 Via Callejon San Clemente, CA 92673
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, October 07, 2011 7:00 PM To: swalck-at-southbaytech.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibarra27-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibarra27-at-gmail.com Name: Maria Lujan Ibarra
Title-Subject: [Filtered] Preparation of TEM cross sections of Semiconductors
Message: Hi
I need to prepare cross section of SiSiO2 for TEM but the epoxy that is in in the lab is very old. I need to buy an adhesive . Can anyone tell me the name of the epoxy that use for cross section and ion milling? I have an adhesive that we use to space applications: down corning 93500, someone suggest to me that I can use it for TEM. Can anyone tell me if its apropiate for that use?
Epotek 353ND is the common epoxy used to glue up x-section sandwiches. It gives a very thin glue line, usually between 1 and 5 microns across. Gatan G1 is the other common epoxy, but in fact it is just re-bottled 353ND. Be sure to mix the two parts by weight using a precision balance and to test the curing before applying the epoxy to your sample. I'm not sure whether the 93-500 is a good substitute for the 353ND. Perhaps you can test the 93-500 by gluing together some pieces of epi-polished, junk silicon and see how well they hold up during grinding and polishing?
Alternatively, you may have success using the micro cleavage technique to obtain high quality cross sections of your samples since you are working with silicon. This requires a deft hand but avoids the hassles of gluing, grinding, polishing, and ion milling, and eliminates sources of artifacts induced by the aforementioned sample prep. steps.
Good luck, Chris
On Fri, Oct 7, 2011 at 9:59 PM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mlibarra27-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: mlibarra27-at-gmail.com Name: Maria Lujan Ibarra } } Title-Subject: [Filtered] Preparation of TEM cross sections of Semiconductors } } Message: Hi } } I need to prepare cross section of SiSiO2 for TEM but the epoxy that is in in the lab is very old. I } need to buy an adhesive . Can anyone tell me the name of the epoxy that use for cross section and } ion milling? } I have an adhesive that we use to space applications: down corning 93500, someone suggest to me that } I can use it for TEM. Can anyone tell me if its apropiate for that use? } } Thanks for your help in advance!! } } Maria Lujan } } } } Login Host: 168.96.65.245 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Oct 7 20:50:04 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p981o4MW029735 } 12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:50:04 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 540F8422B40 } 12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:50:04 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id ERIASHgXvkLS for {microscopy-at-microscopy.com} ; } 12, 25 -- Fri, 7 Oct 2011 20:50:03 -0500 (CDT) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id D7F7F422B35 } 12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:50:03 -0500 (CDT) } 12, 25 -- Message-ID: {4E8FAC4B.7000103-at-microscopy.com} } 12, 25 -- Date: Fri, 07 Oct 2011 20:50:03 -0500 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: mlibarra27-at-gmail.com } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW:Preparation of TEM cross sections of Semiconductors } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 29 -- From microwink-at-gmail.com Fri Oct 7 21:17:27 2011 6, 29 -- Received: from mail-bw0-f41.google.com (mail-bw0-f41.google.com [209.85.214.41]) 6, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p982HQFh004137 6, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 21:17:27 -0500 6, 29 -- Received: by bkbzs2 with SMTP id zs2so6244638bkb.0 6, 29 -- for {Microscopy-at-microscopy.com} ; Fri, 07 Oct 2011 19:17:26 -0700 (PDT) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 29 -- d=gmail.com; s=gamma; 6, 29 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 6, 29 -- :cc:content-type:content-transfer-encoding; 6, 29 -- bh=KxZzP5sqSw5RHQ16RqmMiVXGknCIuYFjibXeyTvQuJ0=; 6, 29 -- b=ECdoVNUfwDMiDQLVRNGbWk/zegzsvQbNEpR7Ysv1npHIBLOq1T21TrT5icseQZsvBD 6, 29 -- 9cw+NkaRr00/1vG0LK/QbFiv9h+DRcQvfGFfK2AHTE9+7R9TL1wcnzwLR+4ib23tcClT 6, 29 -- wi3Xmo0T2fgHzR/6GvRVtvFAqtKKV3ZQDUhlE= 6, 29 -- MIME-Version: 1.0 6, 29 -- Received: by 10.204.128.92 with SMTP id j28mr139541bks.57.1318040244411; Fri, 6, 29 -- 07 Oct 2011 19:17:24 -0700 (PDT) 6, 29 -- Received: by 10.204.41.4 with HTTP; Fri, 7 Oct 2011 19:17:24 -0700 (PDT) 6, 29 -- In-Reply-To: {201110080159.p981xHZQ018390-at-ns.microscopy.com} 6, 29 -- References: {201110080159.p981xHZQ018390-at-ns.microscopy.com} 6, 29 -- Date: Fri, 7 Oct 2011 22:17:24 -0400 6, 29 -- Message-ID: {CAA8T2POrfhgTEFJX+3x+s9tMbt8Y6ssdyZrX7vqM=jxh5Gjc0g-at-mail.gmail.com} 6, 29 -- Subject: Re: [Microscopy] viaWWW:Preparation of TEM cross sections of Semiconductors 6, 29 -- From: Christopher Winkler {microwink-at-gmail.com} 6, 29 -- To: Microscopy-at-microscopy.com 6, 29 -- Cc: mlibarra27-at-gmail.com 6, 29 -- Content-Type: text/plain; charset=ISO-8859-1 6, 29 -- Content-Transfer-Encoding: 8bit 6, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p982HQFh004137 ==============================End of - Headers==============================
The original article by Bravman and Sinclair used "MBond 610" strain gauge epoxy. It formed a very thin glue line (~200nm) and milled at about the same rate as Si. You should be able to purchase MBond 610 from some ot the EM supply houses or from the manufacturer (Vishay Precision Group)
If you are doing conventional ion milling, you want to grind and dimple as thin as possible before ion milling. Also you want to avoid, as much as possible, having the ion beam parallel to the glue line. Some ion mills have a sector control which will either go quickly through the appropriate angles or avoid them altogether. If you ion mill doesn't have a sector control for the rotation, you can put some beam blocks (I used graphite) in line with the glue interface to shield it from the ions.
Cheers, Henk
At 10/7/2011 9:51 PM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } mlibarra27-at-gmail.com as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email:mlibarra27-at-gmail.com Name: Maria Lujan Ibarra } } Title-Subject: [Filtered] Preparation of TEM cross sections of Semiconductors } } Message: Hi } } I need to prepare cross section of SiSiO2 for TEM but the epoxy that is in in the lab is very old. I } need to buy an adhesive . Can anyone tell me the name of the epoxy that use for cross section and } ion milling? } I have an adhesive that we use to space applications: down corning 93500, someone suggest to me that } I can use it for TEM. Can anyone tell me if its apropiate for that use? } } Thanks for your help in advance!! } } Maria Lujan } } } } Login Host: 168.96.65.245 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- Frommicroscopylistserver-noreply-at-microscopy.com Fri Oct 7 20:50:04 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p981o4MW029735 } 12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:50:04 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 540F8422B40 } 12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:50:04 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id ERIASHgXvkLS for {microscopy-at-microscopy.com} ; } 12, 25 -- Fri, 7 Oct 2011 20:50:03 -0500 (CDT) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id D7F7F422B35 } 12, 25 -- for {microscopy-at-microscopy.com} ; Fri, 7 Oct 2011 20:50:03 -0500 (CDT) } 12, 25 -- Message-ID: {4E8FAC4B.7000103-at-microscopy.com} } 12, 25 -- Date: Fri, 07 Oct 2011 20:50:03 -0500 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To:mlibarra27-at-gmail.com } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW:Preparation of TEM cross sections of Semiconductors } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 8, 28 -- From colijn.1-at-osu.edu Sat Oct 8 06:14:18 2011 8, 28 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 8, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p98BEIgd008571 8, 28 -- for {microscopy-at-microscopy.com} ; Sat, 8 Oct 2011 06:14:18 -0500 8, 28 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 8, 28 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 28 -- id {01O6YSKWQWB49410ZL-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 8, 28 -- Sat, 08 Oct 2011 07:14:17 -0400 (EDT) 8, 28 -- Received: from [192.168.1.103] 8, 28 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 8, 28 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 28 -- with ESMTPA id {01O6YSKW1OD293N389-at-ecr6.ohio-state.edu} for 8, 28 -- microscopy-at-microscopy.com; Sat, 08 Oct 2011 07:14:16 -0400 (EDT) 8, 28 -- Date: Sat, 08 Oct 2011 07:14:15 -0400 8, 28 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 8, 28 -- Subject: Re: [Microscopy] viaWWW:Preparation of TEM cross sections of 8, 28 -- Semiconductors 8, 28 -- In-reply-to: {201110080151.p981p0qw032691-at-ns.microscopy.com} 8, 28 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 8, 28 -- To: microscopy-at-microscopy.com 8, 28 -- Message-id: {4E903087.1020804-at-osu.edu} 8, 28 -- MIME-version: 1.0 8, 28 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 28 -- Content-transfer-encoding: 7bit 8, 28 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 8, 28 -- Thunderbird/7.0.1 8, 28 -- X-Env-From: auth/colijn.1-at-osu.edu 8, 28 -- References: {201110080151.p981p0qw032691-at-ns.microscopy.com} ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kaszas.1-at-osu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kaszas.1-at-osu.edu Name: Andrea Kaszas
Organization: Ohio State University/OARDC
Title-Subject: [Filtered] Preparation of cultured cells for TEM
Message: Hi,
We have a researcher who would like to see his swine and chicken cultured cells on the TEM. He cultured the cells on Thermonox Plastic Coverslips Nunc 174950. I made the preparation of these cultured cells for transmission electron microscope according to Carol Heckman's protocol. I embedded them in EM-Bed 812 resin. After polymerization I could not separate the coverslip from the resin. I would like to ask if anybody embedded cultured cells recently? I ask recently, because resins components are changed by the vendor and they do not work the same as before. Any suggestions are highly appreciated. Andrea Kaszas research assistant OSU/OARDC Wooster,OH
Dear All, I tried to mount a Denka M-3 LaB6 cathode to a Philips EM 420 wehnelt ( I also use the same wehnelt configuration in my 525 SEM, interchanging the cathodes, when needed). That`s the first time I tried using Denka cathodes, the last three ones had been Kimball. Can somebody please advice me for the correct heating setting (Kimball had been ca. 12 to 14 on the Philips EM420) and also give me a hint on the correct distance to wehnelt? It seems somewhat different to the Kimball settings and - most disturbing - I can only get a glimpse of green light on the screen during the first 2 or 3 steps of heating, then the cathodes drifts away. I tried this various times, with a wehnelt distance of ca. 0,5 to 0,3mm and re-centering the cathode as good as possible.
Any ideas?
Thanks, Stefan
-- ----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Dera Vitaly, thanks for your important notes. I use a 0.5 mm aperture. I suppose the cathode-wehnelt distance should be ca. 0.7mm ? Do you know if the Denka M-3 needs a lower heating current than the Kimball version? Sure, I know to heat for saturation, but at this state of alignment I can`t see the cathode very well ;-) And I am out of range with the x + y gun tilts, just as you said.
Thanks, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 10.10.11 17:36, schrieb Vitaly Feingold: } Tip to Wehnelt aperture distance depends somewhat on the size of aperture. Safe Wehnelt distance is 0.3mm for a 0.2mm aperture. } You may increase distance of course but this will limit emission current. } } Too short distance especially with larger apertures will cause avalanche-like emission. Impulses of maxed-out emission current } accompanied by flashes of bright light on TEM phosphor screen. A 0.2mm distance with 0.5mm aperture will sure cause this effect. } } I sometimes encounter new cathode that warps when heated. Exactly what you see. Though this was more common for Kimball Physics at } least in my experience. There are 4 ways of dealing with it: } } 1) replace cathode; } } 2) install larger aperture and increase distance proportionaly; } } 3) replace components (resistors and/or potentiometers) in the gun tilt circuit for increasing gun tilt range; } } 4) center cathode perfectly and then start heating it and adjust gun tilt as you go. If gun tilt pot (or both) will max out before } cathode saturation achieved - write down the potentiometers position, turn heating and HT off, pull Wehnelt out and intentionally } de-center cathode tip about 20% to 30% of the radius and note in which direction (any will do for start) and then try again. It } takes me 3 tries at most to find correct position for cathode. Basically you determine where to set the tip so that warping will } put it back to center or close enough to be compensated with gun tilt. } } I note the position by drawing a circle on paper depicting aperture surrounded with numbers 0 to 9 (numbers on Wehnelt cap) and } will put a dot in this circle which marks initial position of the tip. Then write positions of gun tilt potentiometer next to it } after first try. After 2 attempts you will either get it right or will have only one possible direction left. Sort of triangulation. } } Vitaly Feingold } SIA } 2773 Heath Lane } Duluth GA 30096 } Ph. 770-232-7785 } Fax 770-232-1791 } www.sia-cam.com } vitaly-at-sia-cam.com } } On 10/10/2011 11:01 AM, stefan.diller-at-t-online.de wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Dear All, } } I tried to mount a Denka M-3 LaB6 cathode to a Philips EM 420 wehnelt ( I also use the same wehnelt configuration in my 525 SEM, } } interchanging the cathodes, when needed). That`s the first time I tried using Denka cathodes, the last three ones had been Kimball. } } Can somebody please advice me for the correct heating setting (Kimball had been ca. 12 to 14 on the Philips EM420) and also give } } me a hint on the correct distance to wehnelt? It seems somewhat different to the Kimball settings and - most disturbing - I can } } only get a glimpse of green light on the screen during the first 2 or 3 steps of heating, then the cathodes drifts away. I tried } } this various times, with a wehnelt distance of ca. 0,5 to 0,3mm and re-centering the cathode as good as possible. } } } } Any ideas? } } } } Thanks, Stefan } } }
==============================Original Headers============================== 6, 22 -- From stefan.diller-at-t-online.de Mon Oct 10 11:10:13 2011 6, 22 -- Received: from mailout06.t-online.de (mailout06.t-online.de [194.25.134.19]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9AGACcS009688 6, 22 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2011 11:10:12 -0500 6, 22 -- Received: from fwd00.aul.t-online.de (fwd00.aul.t-online.de ) 6, 22 -- by mailout06.t-online.de with smtp 6, 22 -- id 1RDIQZ-0001HJ-BC; Mon, 10 Oct 2011 18:10:07 +0200 6, 22 -- Received: from mac-pro.speedport.ip (EkxTzGZFYh6n40jOSY3WTPU6NO4iudOUGyIx3mzttSAQMbzmwa6ZvzsBNfkUl4eQQX-at-[93.222.97.236]) by fwd00.t-online.de 6, 22 -- with esmtp id 1RDIQX-1DpeDY0; Mon, 10 Oct 2011 18:10:05 +0200 6, 22 -- Message-ID: {4E9318DD.8050803-at-t-online.de} 6, 22 -- Date: Mon, 10 Oct 2011 18:10:05 +0200 6, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 6, 22 -- MIME-Version: 1.0 6, 22 -- To: Vitaly Feingold {vitaly-at-sia-cam.com} , microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] Denka LaB6 cathode mounting 6, 22 -- References: {201110101501.p9AF1IDg023654-at-ns.microscopy.com} {4E9310FD.8080102-at-sia-cam.com} 6, 22 -- In-Reply-To: {4E9310FD.8080102-at-sia-cam.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-ID: EkxTzGZFYh6n40jOSY3WTPU6NO4iudOUGyIx3mzttSAQMbzmwa6ZvzsBNfkUl4eQQX 6, 22 -- X-TOI-MSGID: 309fa69c-2566-4a0c-9930-37b59e530a3c ==============================End of - Headers==============================
The following position is still open for applications at the CAMCOR shared user facility at the University of Oregon.
http://camcor.uoregon.edu/
Please contact John Donovan for details.
mailto:donovan-at-uoregon.edu
The MicroAnalytical Research Assistant position provides professional technical and scientific assistance to support the CAMCOR MicroAnalytical laboratory and associated Inorganic Sample Preparation Laboratory under the direction and instruction of the MicroAnalytical Facility Director. As an entry level position, the MicroAnalytical Research Assistant will be expected to learn all necessary techniques and methods through self-instruction, tutoring, classes, professional development, scientific conferences, and other educational opportunities as available.
The MicroAnalytical Research Assistant will be expected to maintain the instrument laboratory and sample preparation areas, prepare samples of a geological, materials and archeological nature using a variety of sample preparation techniques. The MicroAnalytical Research Assistant will also operate optical and stereo microscopes and perform qualitative and quantitative analysis using a scanning electron microscope (FEI Quanta FEG SEM) and two electron probe micro analysis instruments (Cameca SX50 EPMA and SX100 EPMA).
The MicroAnalytical Research Assistant will also provide assistance, supervision and instruction for students, faculty and commercial visitors using the shared laboratories, optical microscopes, computers, scanners and other shared laboratory equipment. The MicroAnalytical Research Assistant will provide computer backup and file sharing maintenance and support for the MicroAnalytical Facility. Complete details and application procedure at http://academicjobsonline.org.
Duties: Sample Preparation/Coating/Sputtering:
50% Sample Preparation for MicroAnalytical Facility: Cutting, grinding, cleaving, mounting, embedding, and polishing and coating of a wide variety of samples for use in the Microanalytical Facility. Both grain mounts, polished thick and thin sections and standard materials for calibration of the instruments. Vibratory polishing and etching for EBSD samples. Coating using a variety of sputter and evaporation coaters.
Sample preparation using tripod polishing, ultra-microtome, FIB and Electron Beam Lithography will also be required on occasion.
Instrument operation for MicroAnalytical:
30% Instrument Operation for MicroAnalytical Facility: Operate variable pressure FEG SEM and electron microprobes for imaging, mapping and quantitative analysis on a wide variety of samples and materials including thin films, archeological samples, geological samples, and photo-voltaic, semi-conductor, thermo-electric, super conductor and other bulk materials.
Operate energy dispersive spectrometers (EDS), electron backscatter detector (EBSD), cathodo-luminescence (CL) and utilize Peltier cold and hot stages for in-situ SEM experiments.
General facility support and assistance:
10% Computer Backup and Laboratory Maintenance: Manage instrument and shared computers in MicroAnalytical facility. Perform operating system updates, anti-virus updates, file maintenance, access security, user management on secure data servers, purchase parts and consumables for the MicroAnalytical Facility and Inorganic Preparation Laboratory as needed. Work with on-site instrument engineer and electronic engineer as necessary to assist in maintaining all laboratory equipment.
10% Tours, Workshops and Outreach: Conduct technical tours of the facility for community, industry, prospective student and other visitors. Support middle and high school and community college outreach efforts. Organize and support semi-annual workshops and topical conferences for the MicroAnalytical Facility. Other duties as assigned.
Qualifications: Bachelor's degree in a science related field and three years of experience in a laboratory and microscopy related field or Master's degree in a science related field and one year experience in a laboratory and microscopy related field.
Preferred ability in: Experience in sample preparation for EPMA and SEM, including epoxies, adhesives, vacuum impregnation, hot plate mounting with Petropoxy, thin sectioning, polishing with water/alcohol based diamond abrasives.
Operation of metallic/carbon evaporators and sputter coaters, fume hoods, spinners, etchers, and knowledge of proper handling and storing of chemicals.
Technical/scientific programming and computing management and applications. Norton Ghost for automated backup of data storage.
Demonstrated ability in: Following prescribed procedures, communicate effectively verbally and in writing.
Providing efficient and professional laboratory assistance in a fast paced environment. Effectively managing and prioritizing competing demands and keeping shared laboratory facilities at high levels of organization, cleanliness and safety levels. Absorbing a high level of technical details quickly.
The successful candidate will have the ability to work effectively with faculty, staff and students from a variety of diverse backgrounds.
Salary: Salary will be commensurate with qualifications and experience. This is a 0.5 FTE position. There is the possibility that the FTE may increase in the future depending on funding, performance and departmental need.
Application: http://academicjobsonline.org
Please include contact information for three professional references. Review of applications will begin September 27, 2011, and continue until a sufficient pool of qualified applicants is obtained or until the position is filled. This announcement is available in alternate formats upon request. If you are a qualified applicant with a disability and need accommodation with the application process, please call (541) 346-3159. If you need accommodation for the interview, please notify the Business Affairs Office if an interview is scheduled.
An Equal-Opportunity, Affirmative-Action Institution Committed to Cultural Diversity and Compliance with the Americans with Disabilities Act.
John Donovan Director- Microanalytical Facility CAMCOR-UofO 541-346-4632 donovan-at-uoregon.edu www.camcor.uoregon.edu www.epmalab.uoregon.edu
==============================Original Headers============================== 29, 20 -- From donovan-at-uoregon.edu Mon Oct 10 12:31:05 2011 29, 20 -- Received: from smtp.uoregon.edu (oh-mserv4.uoregon.edu [128.223.142.147]) 29, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9AHV48m029954 29, 20 -- for {microscopy-at-microscopy.com} ; Mon, 10 Oct 2011 12:31:05 -0500 29, 20 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 29, 20 -- (authenticated bits=0) 29, 20 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id p9AHV1WB015787 29, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 29, 20 -- Mon, 10 Oct 2011 10:31:02 -0700 29, 20 -- Message-Id: {201110101731.p9AHV1WB015787-at-smtp.uoregon.edu} 29, 20 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 29, 20 -- Date: Mon, 10 Oct 2011 10:30:16 -0700 29, 20 -- To: microscopy-at-microscopy.com 29, 20 -- From: John Donovan {donovan-at-uoregon.edu} 29, 20 -- Subject: MicroAnalytical Research Assistant Search 29, 20 -- Mime-Version: 1.0 29, 20 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 29, 20 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 29, 20 -- definitions=2011-10-10_05:2011-10-10,2011-10-10,1970-01-01 signatures=0 29, 20 -- X-Proofpoint-Spam-Reason: safe ==============================End of - Headers==============================
Up for grabs are 2 MT2-B's and one old style sandbag anti-vibration table.
I cannot guarantee that the MT2-Bs (with the binoculars and little table the binoc's sit on) work, but they would be great for parts.
The sandbag table is in good shape and the sandbags are intact. I know this since I took the table apart and moved each sandbag individually, whew!
If you want them, you must pay for shipping or come and pick them up.
First come, first served.
Thanks,
Paula :-)
-- Paula Sicurello, HTL (ASCP) Supervisor, Electron Microscope Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091
==============================Original Headers============================== 10, 26 -- From patpxs-at-gmail.com Mon Oct 10 14:07:22 2011 10, 26 -- Received: from mail-qw0-f41.google.com (mail-qw0-f41.google.com [209.85.216.41]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9AJ7MfN022436 10, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Oct 2011 14:07:22 -0500 10, 26 -- Received: by qadb17 with SMTP id b17so5306169qad.0 10, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 10 Oct 2011 12:07:22 -0700 (PDT) 10, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 26 -- d=gmail.com; s=gamma; 10, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 10, 26 -- :content-transfer-encoding; 10, 26 -- bh=WkYAlf7SJ4v7eWJ1kVhe2Du+Z7Fqt45y8WyApnwY0QQ=; 10, 26 -- b=uLFP9Xwv76vvL/IcOXaI6iYVvTGWK6MuNayhchVONvLKjgfiI6A5liFRkTmWxBRCMT 10, 26 -- YUde/+i8zGJMLiwGXRSY0lygbaVhOOzNeMfNcmjXa6UokWGFnZXhT3qwTQB5M5zWWbIU 10, 26 -- eSwwu+5Ik1XrffLFc6UyoaqVZoXREJJSYWOPA= 10, 26 -- MIME-Version: 1.0 10, 26 -- Received: by 10.224.9.14 with SMTP id j14mr12065085qaj.83.1318273642087; Mon, 10, 26 -- 10 Oct 2011 12:07:22 -0700 (PDT) 10, 26 -- Received: by 10.229.187.200 with HTTP; Mon, 10 Oct 2011 12:07:22 -0700 (PDT) 10, 26 -- Date: Mon, 10 Oct 2011 15:07:22 -0400 10, 26 -- Message-ID: {CAPSjddZUgXxW0wUaatpNoF1Qdk0L=SGjQQ3S18YDWtZdmJN4hw-at-mail.gmail.com} 10, 26 -- Subject: MT2-B x 2 and sandbag table free to good home 10, 26 -- From: Paula Sicurello {patpxs-at-gmail.com} 10, 26 -- To: Microscopy-at-microscopy.com 10, 26 -- Content-Type: text/plain; charset=ISO-8859-1 10, 26 -- Content-Transfer-Encoding: 8bit 10, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9AJ7MfN022436 ==============================End of - Headers==============================
This is a bit embarrassing, but I've been bothered by this long enough. Check out this picture of the vacuum gauge from my vacuum evaporator:
http://www.mta.ca/dmf/download/jme/imgp0520a.jpg
My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My feeling for typical vacuum system performance tells me it's the former, but the labeling on the gauge is somewhat ambiguous. Does the large "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems I've used didn't leave this open to interpretation.
Thanks in advance to those who respond and help dispel my uneasiness...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
My money is on 2E-5 Torr, based on layout of the scale and min/max values....
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/12/2011 9:11 AM, jehrman-at-mta.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } This is a bit embarrassing, but I've been bothered by this long enough. } Check out this picture of the vacuum gauge from my vacuum evaporator: } } http://www.mta.ca/dmf/download/jme/imgp0520a.jpg } } My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My } feeling for typical vacuum system performance tells me it's the former, } but the labeling on the gauge is somewhat ambiguous. Does the large } "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems } I've used didn't leave this open to interpretation. } } Thanks in advance to those who respond and help dispel my uneasiness... } } Jim } }
==============================Original Headers============================== 4, 40 -- From vray-at-partbeamsystech.com Wed Oct 12 08:23:47 2011 4, 40 -- Received: from nm3-vm0.bullet.mail.sp2.yahoo.com (nm3-vm0.bullet.mail.sp2.yahoo.com [98.139.90.230]) 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9CDNlOW001991 4, 40 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 08:23:47 -0500 4, 40 -- Received: from [98.139.91.61] by nm3.bullet.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 13:23:46 -0000 4, 40 -- Received: from [98.139.91.42] by tm1.bullet.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 13:23:46 -0000 4, 40 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 13:23:46 -0000 4, 40 -- X-Yahoo-Newman-Id: 499143.48176.bm-at-omp1042.mail.sp2.yahoo.com 4, 40 -- Received: (qmail 15088 invoked from network); 12 Oct 2011 13:23:45 -0000 4, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1318425825; bh=8GBPIQNZ5vPp/5VX9ulrylHRfM1c2fAlElz67+Wvxh4=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=pA02S8x9g88wlQuvTkrDfbo35CO6llFZKhCn3N+ECSh7hy5MEK+FSf7NwpGTmsIp9pDs4tWdlFNroT3zJ+CVtDY2bOjjh0Tzykh2xBbfwCD48JjwJXy3FkWprRPFUJSVwTLkqpuallMRBhEu+vRuBOTN3PnRgNc/MVKlvL/QVFI= 4, 40 -- X-Yahoo-Newman-Property: ymail-3 4, 40 -- X-YMail-OSG: UgjhskcVM1lXO3fG18uoHR7.oqJv7CbWVanwExYJkk4u.5. 4, 40 -- mawVfPTaVGg2IIgdESeykRRYQIvZfcGtCVmj28W3RLby7REPthKGfHr1fZNm 4, 40 -- jEdbTvE5l1ydVyoYRXkdsd1wH8Bq3XWwp0u3ExXKynGGmU4r9I3djTHeOpMZ 4, 40 -- OsN8awVUszVvInobyEl7cr4OUwSZkEiFJl5W8TRpos2B_QokVZHwVOJx7uRy 4, 40 -- 9rHyuSykPIoFko9uh7e4aJ7lmAZIlIV5h_83GE63T1IxkYjV.xqxQzyFQWnB 4, 40 -- smoN9kT9hkDmr3GA3tRMgc18RYfcHf67XFY7Bl9cbGWq54epmVFtTKP2S8VF 4, 40 -- t1u7jB8uXVNyjBDb_zQxrA1RgC2.yecxWqhJTW2uqxOgWuK2q3rWJxqEcaL6 4, 40 -- K3HIgMR1FPEXc2AG5Mr4D6BmoCkkPjDER8HYmegDPLznsh5P9cjBQUfKSCmf 4, 40 -- .DP8JbcweiMdXC6LGWvNnjFBBmvIc.OSvYDWP1n3fXr5.2LXSEeA2ejOhg7m 4, 40 -- .X1ExhF3SyZ1NhUZu3FEMZmibZE67Rd7ydPvStrudQ4pB.xEPwq_9tjDJ4aS 4, 40 -- HZCMvryfR.X8.mV2WPnCjssQuAqUPuHuf6.JAvkzYAPTb7geFODvdwJBa0ax 4, 40 -- U_ZPgXWj9.iP7 4, 40 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 40 -- Received: from [192.168.1.109] (vray-at-71.174.120.150 with plain) 4, 40 -- by smtp113.biz.mail.mud.yahoo.com with SMTP; 12 Oct 2011 06:23:45 -0700 PDT 4, 40 -- Message-ID: {4E9594E4.1080306-at-partbeamsystech.com} 4, 40 -- Date: Wed, 12 Oct 2011 09:23:48 -0400 4, 40 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 40 -- Reply-To: vray-at-partbeamsystech.com 4, 40 -- Organization: PBS&T 4, 40 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 4, 40 -- MIME-Version: 1.0 4, 40 -- To: jehrman-at-mta.ca 4, 40 -- CC: microscopy-at-microscopy.com 4, 40 -- Subject: Re: [Microscopy] vacuum gauge reading 4, 40 -- References: {201110121311.p9CDBx1Q020034-at-ns.microscopy.com} 4, 40 -- In-Reply-To: {201110121311.p9CDBx1Q020034-at-ns.microscopy.com} 4, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 6, 26 -- From frank_karl-at-ardl.com Wed Oct 12 08:26:18 2011 6, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CDQG7O007791 6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 08:26:18 -0500 6, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 6, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Wed, 12 6, 26 -- Oct 2011 09:26:14 -0400 6, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 6, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 6, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 6, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 6, 26 -- Date: Wed, 12 Oct 2011 09:26:14 -0400 6, 26 -- Subject: IGNORE- Looking for out of town messages- Firewall changed TEST 6, 26 -- Thread-Topic: IGNORE- Looking for out of town messages- Firewall changed 6, 26 -- TEST 6, 26 -- Thread-Index: AcyI4oOjqh3cwv7PQ3OlRrpPTD2LuQ== 6, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AFBD-at-exchange2k7.ad.ardl.com} 6, 26 -- Accept-Language: en-US 6, 26 -- Content-Language: en-US 6, 26 -- X-MS-Has-Attach: 6, 26 -- X-MS-TNEF-Correlator: 6, 26 -- acceptlanguage: en-US 6, 26 -- Content-Type: text/plain; charset="iso-8859-1" 6, 26 -- MIME-Version: 1.0 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CDQG7O007791 ==============================End of - Headers==============================
its a logarithmic scale. The big 10-5 means 10 x 10-6. So the shown value is 2x 10-6 Torr.
Jo
Am 12.10.2011 15:26, schrieb vray-at-partbeamsystech.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } My money is on 2E-5 Torr, based on layout of the scale and min/max } values.... } } Cheers :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 10/12/2011 9:11 AM, jehrman-at-mta.ca wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi Listers, } } } } This is a bit embarrassing, but I've been bothered by this long enough. } } Check out this picture of the vacuum gauge from my vacuum evaporator: } } } } http://www.mta.ca/dmf/download/jme/imgp0520a.jpg } } } } My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My } } feeling for typical vacuum system performance tells me it's the former, } } but the labeling on the gauge is somewhat ambiguous. Does the large } } "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems } } I've used didn't leave this open to interpretation. } } } } Thanks in advance to those who respond and help dispel my uneasiness... } } } } Jim } } } } } }
-- Johannes Bernardi University Service Centre for Electron Microscopy Vienna University of Technology Austria
==============================Original Headers============================== 6, 20 -- From bernardi-at-tuwien.ac.at Wed Oct 12 08:41:06 2011 6, 20 -- Received: from mail1.zserv.tuwien.ac.at (mail1.zserv.tuwien.ac.at [128.130.35.37]) 6, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CDf5ju004220 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 08:41:05 -0500 6, 20 -- Received: from [128.130.45.81] (baloo.atp.tuwien.ac.at [128.130.45.81]) 6, 20 -- by mail1.zserv.tuwien.ac.at (8.13.8/8.13.8) with ESMTP id p9CDf4UK030533 6, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 15:41:05 +0200 6, 20 -- Message-ID: {4E9598F0.6080203-at-tuwien.ac.at} 6, 20 -- Date: Wed, 12 Oct 2011 15:41:04 +0200 6, 20 -- From: Johannes Bernardi {bernardi-at-tuwien.ac.at} 6, 20 -- Reply-To: bernardi-at-tuwien.ac.at 6, 20 -- Organization: TU Wien, Austria 6, 20 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 6, 20 -- MIME-Version: 1.0 6, 20 -- To: Microscopy-at-microscopy.com 6, 20 -- Subject: Re: [Microscopy] Re: vacuum gauge reading 6, 20 -- References: {201110121326.p9CDQxBe007992-at-ns.microscopy.com} 6, 20 -- In-Reply-To: {201110121326.p9CDQxBe007992-at-ns.microscopy.com} 6, 20 -- Content-Type: text/plain; charset=ISO-8859-15; format=flowed 6, 20 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
On Wed, Oct 12, 2011 at 9:12 AM, {jehrman-at-mta.ca} wrote: } } http://www.mta.ca/dmf/download/jme/imgp0520a.jpg } } My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6?
2x10-6, or 2E-6 torr
} } Does the large } "10-5" on the meter indicate 10x10-5, or 1x10-5?
10-5 = 1x10-5, also expressed as 1E-5
In standard scientific notation, the prefix ("significand") is } =1, but {10. Therefore, when you get to 10x10-6 you would re-write it as 1x10-5
Jim Passmore Research Associate Sealed Air Corporation james.passmore-at-sealedair.com 864-433-2927 voice 864-433-2205 fax
==============================Original Headers============================== 9, 22 -- From james.passmore-at-sealedair.com Wed Oct 12 09:02:38 2011 9, 22 -- Received: from exprod5og111.obsmtp.com (exprod5og111.obsmtp.com [64.18.0.22]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CE2cYH021319 9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 09:02:38 -0500 9, 22 -- Received: from mail-vx0-f180.google.com ([209.85.220.180]) (using TLSv1) by exprod5ob111.postini.com ([64.18.4.12]) with SMTP; 9, 22 -- Wed, 12 Oct 2011 07:02:38 PDT 9, 22 -- Received: by vcbf11 with SMTP id f11so871573vcb.11 9, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 07:02:37 -0700 (PDT) 9, 22 -- Received: by 10.52.20.174 with SMTP id o14mr23609961vde.7.1318428157234; Wed, 9, 22 -- 12 Oct 2011 07:02:37 -0700 (PDT) 9, 22 -- MIME-Version: 1.0 9, 22 -- Received: by 10.52.157.100 with HTTP; Wed, 12 Oct 2011 07:01:57 -0700 (PDT) 9, 22 -- In-Reply-To: {201110121312.p9CDCawF021038-at-ns.microscopy.com} 9, 22 -- References: {201110121312.p9CDCawF021038-at-ns.microscopy.com} 9, 22 -- From: "James (Jim) Passmore" {james.passmore-at-sealedair.com} 9, 22 -- Date: Wed, 12 Oct 2011 10:01:57 -0400 9, 22 -- Message-ID: {CAGV-iYaupt0RNaPBUcze9KQmopqmvqHvTghu5MzV_wJqx6zYzg-at-mail.gmail.com} 9, 22 -- Subject: Re: [Microscopy] vacuum gauge reading 9, 22 -- To: Microscopy-at-microscopy.com 9, 22 -- Content-Type: text/plain; charset=ISO-8859-1 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CE2cYH021319 ==============================End of - Headers==============================
I have a Denton system that uses the same vacuum gauge. The vacuum reading shown is slightly less than 2 x10-5 torr. The right to left deflection of the needle seems to cause problems for many users.
John A. Robson Research Scientist Boehringer Ingelheim Pharmaceuticals, Inc.
-----Original Message----- X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] Sent: Wednesday, October 12, 2011 9:23 AM To: Robson,John (AN) BIP-US-R
Hi Listers,
This is a bit embarrassing, but I've been bothered by this long enough. Check out this picture of the vacuum gauge from my vacuum evaporator:
http://www.mta.ca/dmf/download/jme/imgp0520a.jpg
My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My feeling for typical vacuum system performance tells me it's the former, but the labeling on the gauge is somewhat ambiguous. Does the large "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems I've used didn't leave this open to interpretation.
Thanks in advance to those who respond and help dispel my uneasiness...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Seems like you have 2 received opinions on either side.
Without checking any more definitive resource, I would vote for 2x10-6. I have always understood the large numbers to be 1x10-y. The numbers in between would be added to the large label on their left.
Warren
-----Original Message----- X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] Sent: Wednesday, October 12, 2011 8:12 AM To: wesaia-at-iastate.edu
Hi Listers,
This is a bit embarrassing, but I've been bothered by this long enough. Check out this picture of the vacuum gauge from my vacuum evaporator:
http://www.mta.ca/dmf/download/jme/imgp0520a.jpg
My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My feeling for typical vacuum system performance tells me it's the former, but the labeling on the gauge is somewhat ambiguous. Does the large "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems I've used didn't leave this open to interpretation.
Thanks in advance to those who respond and help dispel my uneasiness...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Cast my vote for 2X10-5. Here's why. The air pressure is getting smaller, the amount of air is shrinking, if you will. It was at 10-3 and shrunk to 10-4 and now to 10-5. It's not "small" enough to be at 10-6, but it's smaller than 8x10-5, in fact it's around 2.2x10-5. I don't mean to be insulting, but this is how I think of these scales. They aren't negative numbers, but they are below "zero" in our ordinary experience.
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Wednesday, October 12, 2011 10:50 AM To: Frank Karl
Seems like you have 2 received opinions on either side.
Without checking any more definitive resource, I would vote for 2x10-6. I have always understood the large numbers to be 1x10-y. The numbers in between would be added to the large label on their left.
Warren
-----Original Message----- X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] Sent: Wednesday, October 12, 2011 8:12 AM To: wesaia-at-iastate.edu
Hi Listers,
This is a bit embarrassing, but I've been bothered by this long enough. Check out this picture of the vacuum gauge from my vacuum evaporator:
http://www.mta.ca/dmf/download/jme/imgp0520a.jpg
My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My feeling for typical vacuum system performance tells me it's the former, but the labeling on the gauge is somewhat ambiguous. Does the large "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems I've used didn't leave this open to interpretation.
Thanks in advance to those who respond and help dispel my uneasiness...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 34, 26 -- From frank_karl-at-ardl.com Wed Oct 12 10:03:52 2011 34, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 34, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CF3qPD007466 34, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 10:03:52 -0500 34, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 34, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Wed, 12 34, 26 -- Oct 2011 11:03:51 -0400 34, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 34, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 34, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 34, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 34, 26 -- Date: Wed, 12 Oct 2011 11:03:50 -0400 34, 26 -- Subject: RE: [Microscopy] RE: vacuum gauge reading 34, 26 -- Thread-Topic: [Microscopy] RE: vacuum gauge reading 34, 26 -- Thread-Index: AcyI7jAR1n/4qfdBSt+NKHCJeTIOjgAAESaQ 34, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AFBE-at-exchange2k7.ad.ardl.com} 34, 26 -- In-Reply-To: {201110121449.p9CEnZQ1006113-at-ns.microscopy.com} 34, 26 -- Accept-Language: en-US 34, 26 -- Content-Language: en-US 34, 26 -- X-MS-Has-Attach: 34, 26 -- X-MS-TNEF-Correlator: 34, 26 -- acceptlanguage: en-US 34, 26 -- Content-Type: text/plain; charset="iso-8859-1" 34, 26 -- MIME-Version: 1.0 34, 26 -- Content-Transfer-Encoding: 8bit 34, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CF3qPD007466 ==============================End of - Headers==============================
its a logarithmic scale. The big 10-5 means 10 x 10-6. So the shown value is 2x 10-6 Torr.
Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 6, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Wed Oct 12 10:10:20 2011 6, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CFAKpt015897 6, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 10:10:20 -0500 6, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 6, 25 -- by localhost (Postfix) with SMTP id 51187531C 6, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 17:10:17 +0200 (CEST) 6, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 6, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 3A9BB52BD 6, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 17:10:17 +0200 (CEST) 6, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 6, 25 -- with Novell_GroupWise; Wed, 12 Oct 2011 17:10:17 +0200 6, 25 -- Message-Id: {4E95C9F7020000540002D153-at-gwsmtp1.uni-regensburg.de} 6, 25 -- X-Mailer: Novell GroupWise Internet Agent 12.0.0 Beta 6, 25 -- Date: Wed, 12 Oct 2011 17:10:15 +0200 6, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 6, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 6, 25 -- Subject: vacuum gauge reading 6, 25 -- References: {201110121341.p9CDfIF0004536-at-ns.microscopy.com} 6, 25 -- In-Reply-To: {201110121341.p9CDfIF0004536-at-ns.microscopy.com} 6, 25 -- Mime-Version: 1.0 6, 25 -- Content-Type: text/plain; charset=US-ASCII 6, 25 -- Content-Disposition: inline 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CFAKpt015897 ==============================End of - Headers==============================
IMHO: practically (and logically) the scale is read from the right edge to the left …. so if pressure is at {rough} value (poor vacuum), say “under 10^-3†(10^-2†not included in the scale) and reaches at some time == } 8 x 10^-3(Torr), the index needle (likely) overcasts/covers the first scale 'bar' at the most right side of the scale.…..
Following decreasing pressure in the recipient, you’ll get to/at 1. 10^-3 Torr, then 8 x 10^-4, 6x 10^-4, 4x 10^-4 Torr, and so forth, respectively…
So in my honest opinion, the value shown in the image correctly should be read as:
((☺) approx. ) 2.125 (since depiction/distances of bars is/are logarithmic) x 10^-6 Torr (as some other have pointed out too but only without decimal digits... joke!)
best wishes and greetings
Wolfgang MUSS SALZBURG-AUSTRIA
Von: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] Gesendet: Mittwoch, 12. Oktober 2011 15:15 An: Muß Wolfgang Betreff: [Microscopy] Vacuum gauge reading
--------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi Listers,
This is a bit embarrassing, but I've been bothered by this long enough. Check out this picture of the vacuum gauge from my vacuum evaporator:
http://www.mta.ca/dmf/download/jme/imgp0520a.jpg
My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6?
My feeling for typical vacuum system performance tells me it's the former, but the labeling on the gauge is somewhat ambiguous. Does the large "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems I've used didn't leave this open to interpretation.
Thanks in advance to those who respond and help dispel my uneasiness...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Hmmmm.....maybe MSA should have an afternoon tutorial next year on how to read vacuum gauges.
John Mardinly ASU
On Oct 12, 2011, at 6:23 AM, "jehrman-at-mta.ca" {jehrman-at-mta.ca} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } This is a bit embarrassing, but I've been bothered by this long enough. } Check out this picture of the vacuum gauge from my vacuum evaporator: } } http://www.mta.ca/dmf/download/jme/imgp0520a.jpg } } My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My } feeling for typical vacuum system performance tells me it's the former, } but the labeling on the gauge is somewhat ambiguous. Does the large } "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems } I've used didn't leave this open to interpretation. } } Thanks in advance to those who respond and help dispel my uneasiness... } } Jim } } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } You are what you eat. } So stay away from the jerk chicken. } } } ==============================Original Headers============================== } 12, 19 -- From jehrman-at-mta.ca Wed Oct 12 08:10:34 2011 } 12, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.4]) } 12, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CDAYA4018700 } 12, 19 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 08:10:34 -0500 } 12, 19 -- Received: from host-22-194.mta.ca ([138.73.22.194]:55925) } 12, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) } 12, 19 -- (Exim 4.76) } 12, 19 -- (envelope-from {jehrman-at-mta.ca} ) } 12, 19 -- id 1RDyZt-0000aw-KK } 12, 19 -- for Microscopy-at-microscopy.com; Wed, 12 Oct 2011 10:10:33 -0300 } 12, 19 -- Message-ID: {4E9591C7.1000606-at-mta.ca} } 12, 19 -- Date: Wed, 12 Oct 2011 10:10:31 -0300 } 12, 19 -- From: James Ehrman {jehrman-at-mta.ca} } 12, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 } 12, 19 -- MIME-Version: 1.0 } 12, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} } 12, 19 -- Subject: vacuum gauge reading } 12, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 19 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 39 -- From John.Mardinly-at-asu.edu Wed Oct 12 10:57:03 2011 5, 39 -- Received: from bcnet7.asu.edu (bcnet7.asu.edu [129.219.117.222]) 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CFv2AY025822 5, 39 -- for {Microscopy-at-Microscopy.com} ; Wed, 12 Oct 2011 10:57:02 -0500 5, 39 -- X-ASG-Debug-ID: 1318434998-03608c0344492020006-FOsErg 5, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet7.asu.edu with ESMTP id 61qpt8kjGJTFMKX0 (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Wed, 12 Oct 2011 08:56:55 -0700 (MST) 5, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 5, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 5, 39 -- X-ASG-Whitelist: Client 5, 39 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.196) by 5, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 5, 39 -- 8.2.255.0; Wed, 12 Oct 2011 08:56:47 -0700 5, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.205]) by 5, 39 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Wed, 12 Oct 5, 39 -- 2011 08:56:47 -0700 5, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 5, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Wed, 12 Oct 2011 5, 39 -- 15:56:46 +0000 5, 39 -- Subject: Re: [Microscopy] vacuum gauge reading 5, 39 -- References: {201110121323.p9CDNZdb001693-at-ns.microscopy.com} 5, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] vacuum gauge reading 5, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} 5, 39 -- Thread-Topic: [Microscopy] vacuum gauge reading 5, 39 -- Thread-Index: AcyI94uWy+C9dBaUQ0S/6fskqkQQEQ== 5, 39 -- Content-Type: text/plain; charset="us-ascii" 5, 39 -- In-Reply-To: {201110121323.p9CDNZdb001693-at-ns.microscopy.com} 5, 39 -- Message-ID: {1225952D-8A23-4AFE-8341-2FB19E367CC0-at-exchange.asu.edu} 5, 39 -- Date: Wed, 12 Oct 2011 09:01:59 -0700 5, 39 -- To: {jehrman-at-mta.ca} , MSA Listserver {Microscopy-at-Microscopy.com} 5, 39 -- CC: {John.Mardinly-at-asu.edu} 5, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) 5, 39 -- X-OriginalArrivalTime: 12 Oct 2011 15:56:47.0144 (UTC) FILETIME=[8BD8C280:01CC88F7] 5, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 5, 39 -- X-Barracuda-Start-Time: 1318435014 5, 39 -- X-Barracuda-Encrypted: RC4-MD5 5, 39 -- X-Barracuda-URL: http://129.219.117.222:8000/cgi-mod/mark.cgi 5, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu 5, 39 -- Content-Transfer-Encoding: 8bit 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CFv2AY025822 ==============================End of - Headers==============================
I've been keeping a tally of responses - so far 10-6 is ahead of 10-5, but not by much and not statistically significantly so. IMHO all responses have made perfect sense - the same way that my schizophrenic confusion on one hand says one value is right but then the other says it certainly must be the other value. I think the only resolution will come from:
1. Asking Denton directly (hopefully I won't get two different responses!) 2. More definitively, rounding up a McLeod gauge.
Another possible solution would be to measure the actual voltage going through the cold cathode head - if only I knew what the values represented! According to the schematics, this is a Balzers IKR 250 cold cathode gauge. Does anybody know what the ballpark voltages should be for the two pressures?
I'll let you know what, if anything I find out. Bad news is, half of you are going to be disappointed.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
With all humility and respect, it is plain that Jim is not the only one confused by these gauges. I have stared at a meter for many minutes many times as I tried to sort this out in my own head.
Vacuum gauges, whether measuring current, voltage, resistance, etc. are just like most other meters: The lower, or smaller, or least magnitude values are on the left, the larger on the right. It is just that the pressure gauge starts with the needle pointing to the larger value on the right (closer to atmospheric pressure), and moves to the smaller value end of the meter on the left as the pumps improve the vacuum. We are more accustomed to most other sorts of gauges in which the needle starts by pointing to the smaller magnitude on the left and moves left to right as the gadget it is attached to does its stuff.
Therefore, the vacuum meter reading, as the needle moves to the left of the tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the larger magnitude to the right of the smaller magnitude. The meter in Jim's image is reading just a hair higher than 2 x 10e-6.
Of course, not content to leave well enough alone, we create most of our own confusion when we refer to "higher vacuum" as the state when the magnitude of pressure is "lower", and "lower vacuum" when the pressure reading is of "higher" magnitude. But that is another thread...
With smiles Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CGopcD011522 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 (EDT) 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 9, 21 -- Subject: Vacuum gauge reading 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} 9, 21 -- Thread-Topic: Vacuum gauge reading 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== 9, 21 -- Mime-version: 1.0 9, 21 -- Content-type: text/plain; 9, 21 -- charset="US-ASCII" 9, 21 -- Content-transfer-encoding: 7bit 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 9, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
This is an interesting question and has generated a bunch of interesting answers. Traditionally, the 10-x point would be interpreted as the upper end of the 10-x range, so that on pumping down, when you pass below the 10-x point you are into the 10-x range. On this basis the reading shown would be 2 x 10-5 Torr.
However, just to get an authoritative answer I have forwarded Joim's original message to info-at-dentonvacuum.com. Let's see what response we get. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 15 -- From bigelow-at-umich.edu Wed Oct 12 12:26:41 2011 3, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 3, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CHQfnN029690 3, 15 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:26:41 -0500 3, 15 -- Received: FROM [76.226.9.147] (adsl-76-226-9-147.dsl.sfldmi.sbcglobal.net [76.226.9.147]) 3, 15 -- By hackers.mr.itd.umich.edu ID 4E95CDD0.68275.25452 ; 3, 15 -- Authuser bigelow; 3, 15 -- 12 Oct 2011 13:26:40 EDT 3, 15 -- Mime-Version: 1.0 3, 15 -- Message-Id: {p06240802cabb7cd686d6-at-[76.226.9.147]} 3, 15 -- Date: Wed, 12 Oct 2011 13:26:24 -0400 3, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 15 -- Subject: [Microscopy]RE; Vac Gauge reading 3, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
OK, I want to reply to this response, not that Roger is saying anything wrong, but just that he along with several others are misunderstanding my question, in several different ways. A few points:
1. Yes, this vacuum gauge, like a lot of others (but certainly not all) work "backward". I completely understand this. The pumping system starts out at poor (= high pressure = more air/gas) with the needle on the gauge pointing to the right. As the vacuum improves (= lower pressure = less air/gas) the needle moves slowly to the left. Some who are only shown my image and haven't seen the needle move might try to read the gauge from left to right and get the order of magnitude of the reading wrong. But I'm not doing that, and this isn't my question/problem.
2. The essence of the problem is the labeling (or lack thereof) on the gauge. What boundary does the large "10-5" represent? In "official" scientific notation (as several responders have pointed out) this label should refer to 1x10-5, in which case, the gauge needle moving right to left as the vacuum improves to the time the image was captured would indicate ~2x10-6. It is very natural and certainly understandable to interpret the gauge this way. But there is a different interpretation (apparently the position of several other responders). The label "10-5" could symbolize "you are now entering the 10-5 range" (remembering that the needle moves from right to left as vacuum improves), in which case the label refers to 10x10-5 = 1X10-4. Under this scheme the gauge in my picture is reading ~2x10-5. This is also a perfectly understandable interpretation. If I look at a digital vacuum gauge (like on our SEM) and it drops down to 9.9x10-5, I'm comfortable thinking that the vacuum has entered the 10-5 range, even though it physically has more in common with a 10-4 vacuum.
3. In my initial message I wrote that I suspected that 2x10-5 was more likely based on the performance of other vacuum systems I've known. The image was taken 5 or 10 minutes after the main valve was opened on the vacuum evaporator, from a cold start, with no LN2 in the cold trap. 2x10-5 seems to be a reasonable vacuum under these circumstances, but 2x10-6 would seem less probable. Adding LN2 to the cold trap drops the vacuum down into the next leftmost range (either 10-6 or 10-7, depending on your interpretation of the labeling). Neither of these possible vacuums is impossible, but a 10-6 value in the relatively early stages of cryopumping would seem the more likely. So my feeling based on the behavior of the pumps themselves was that "10-5" actually indicates 10x10-5 and not 1x10-5. But others (both in this thread and visitors to the lab) have insisted the opposite, i.e. that the gauge reading in my picture must be 2x10-6. Hence my dilemma.
I hope this clarifies things.
JME
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
"It's 5:50 a.m., Do you know where your stack pointer is?"
On 12/10/2011 1:51 PM, raristau-at-ims.uconn.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This has been a fun thread to follow.... } } With all humility and respect, it is plain that Jim is not the only one } confused by these gauges. I have stared at a meter for many minutes many } times as I tried to sort this out in my own head. } } Vacuum gauges, whether measuring current, voltage, resistance, etc. are just } like most other meters: The lower, or smaller, or least magnitude values are } on the left, the larger on the right. It is just that the pressure gauge } starts with the needle pointing to the larger value on the right (closer to } atmospheric pressure), and moves to the smaller value end of the meter on } the left as the pumps improve the vacuum. We are more accustomed to most } other sorts of gauges in which the needle starts by pointing to the smaller } magnitude on the left and moves left to right as the gadget it is attached } to does its stuff. } } Therefore, the vacuum meter reading, as the needle moves to the left of the } tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note } that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the } larger magnitude to the right of the smaller magnitude. The meter in Jim's } image is reading just a hair higher than 2 x 10e-6. } } Of course, not content to leave well enough alone, we create most of our own } confusion when we refer to "higher vacuum" as the state when the magnitude } of pressure is "lower", and "lower vacuum" when the pressure reading is of } "higher" magnitude. But that is another thread... } } With smiles } Roger } } Roger A. Ristau, PhD } Electron Microscopy Specialist } Institute of Materials Science } 97 North Eagleville Road } University of Connecticut } Storrs, CT 06269 } vox: 860-486-5453 } fax: 860-486-4745 } } } } } ==============================Original Headers============================== } 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 } 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) } 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CGopcD011522 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 } 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) } 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 (EDT) } 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 } 9, 21 -- Subject: Vacuum gauge reading } 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} } 9, 21 -- Thread-Topic: Vacuum gauge reading } 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== } 9, 21 -- Mime-version: 1.0 } 9, 21 -- Content-type: text/plain; } 9, 21 -- charset="US-ASCII" } 9, 21 -- Content-transfer-encoding: 7bit } 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 } 9, 21 -- X-Virus-Status: Clean } ==============================End of - Headers==============================
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
With all humility and respect, it is plain that Jim is not the only one confused by these gauges. I have stared at a meter for many minutes many times as I tried to sort this out in my own head.
Vacuum gauges, whether measuring current, voltage, resistance, etc. are just like most other meters: The lower, or smaller, or least magnitude values are on the left, the larger on the right. It is just that the pressure gauge starts with the needle pointing to the larger value on the right (closer to atmospheric pressure), and moves to the smaller value end of the meter on the left as the pumps improve the vacuum. We are more accustomed to most other sorts of gauges in which the needle starts by pointing to the smaller magnitude on the left and moves left to right as the gadget it is attached to does its stuff.
Therefore, the vacuum meter reading, as the needle moves to the left of the tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the larger magnitude to the right of the smaller magnitude. The meter in Jim's image is reading just a hair higher than 2 x 10e-6.
Of course, not content to leave well enough alone, we create most of our own confusion when we refer to "higher vacuum" as the state when the magnitude of pressure is "lower", and "lower vacuum" when the pressure reading is of "higher" magnitude. But that is another thread...
With smiles Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CGopcD011522 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 (EDT) 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 9, 21 -- Subject: Vacuum gauge reading 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} 9, 21 -- Thread-Topic: Vacuum gauge reading 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== 9, 21 -- Mime-version: 1.0 9, 21 -- Content-type: text/plain; 9, 21 -- charset="US-ASCII" 9, 21 -- Content-transfer-encoding: 7bit 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 9, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
------ End of Forwarded Message
==============================Original Headers============================== 20, 22 -- From raristau-at-ims.uconn.edu Wed Oct 12 12:48:50 2011 20, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 20, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CHmnv7023637 20, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:48:49 -0500 20, 22 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 20, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id ADD202CFBF 20, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:48:49 -0400 (EDT) 20, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 20, 22 -- Date: Wed, 12 Oct 2011 13:48:48 -0400 20, 22 -- Subject: FW: [Microscopy] Vacuum gauge reading 20, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 20, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 20, 22 -- Message-ID: {CABB4B40.321B%raristau-at-ims.uconn.edu} 20, 22 -- Thread-Topic: [Microscopy] Vacuum gauge reading 20, 22 -- Thread-Index: AcyJBzHKcDslHPT6EeCBZAAbY55CVA== 20, 22 -- In-Reply-To: {201110121653.p9CGrCU8017189-at-ns.microscopy.com} 20, 22 -- Mime-version: 1.0 20, 22 -- Content-type: text/plain; 20, 22 -- charset="US-ASCII" 20, 22 -- Content-transfer-encoding: 7bit 20, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 20, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I would report the vacuum reading as 2 x 10-5 Torr. That's what I was consistently taught by service engineers and professional microscopists over many years.
We have a Denton vacuum evaporator (oil diffusion pump) with a large bell jar. Under ideal conditions, it can achieve a final vacuum of 2 x 10-5 (just like the reading shown in the photo). That's what is stated in the manual that came with it. If one adds liquid nitrogen to the trap, it can enter the 10-6 range and ultimately reach 2-3 x 10-6 Torr.
So, if the photo is from a diffusion pumped system (without LN2), then 2 x 10-5 would be the most likely reading (just based on ultimate capability alone).
I am enjoying this discussion. It just points out we do not always agree on things in the realm of science. We can all learn from this.
John Bozzola
==============================Original Headers============================== 5, 18 -- From bozzola-at-siu.edu Wed Oct 12 12:59:12 2011 5, 18 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CHxBSL015258 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:59:12 -0500 5, 18 -- Received: by vws16 with SMTP id 16so1043619vws.0 5, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 10:59:11 -0700 (PDT) 5, 18 -- MIME-Version: 1.0 5, 18 -- Received: by 10.52.6.132 with SMTP id b4mr162373vda.39.1318442351638; Wed, 12 5, 18 -- Oct 2011 10:59:11 -0700 (PDT) 5, 18 -- Received: by 10.52.109.169 with HTTP; Wed, 12 Oct 2011 10:59:11 -0700 (PDT) 5, 18 -- In-Reply-To: {201110121744.p9CHiQkR015378-at-ns.microscopy.com} 5, 18 -- References: {201110121744.p9CHiQkR015378-at-ns.microscopy.com} 5, 18 -- Date: Wed, 12 Oct 2011 12:59:11 -0500 5, 18 -- Message-ID: {CAKeCJ9yN-mfGkPne+wC+aH5FsDkKnN0-5uFF2e286py0goKLBQ-at-mail.gmail.com} 5, 18 -- Subject: Re: [Microscopy] Re: Vacuum gauge reading 5, 18 -- From: John Bozzola {bozzola-at-siu.edu} 5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 18 -- Content-Type: text/plain; charset=UTF-8 ==============================End of - Headers==============================
Okay, I'm having fun with this so let me boot in another thought experiment.
Imagine we're at the far right of the meter at 760 torr. Lower the pressure and the meter reads 759. Lets do this until we reach 1 torr. lower the pressure to 0.9 torr and the meter would read 9x10-1. Lower the pressure more and the mete reads 1x10-1. So far so good. Another step and we are at 0.9X10-1 or 9X10-2. Repeat that process enough and we cross over from 1 x10-4 to 0.9x10-4 or 9x10-5 eventually we get to our needle reading.
This makes 4 cents I've kicked in and by now you are forewarned. As the experts say to be forewarned is forearmed. Four arms is both an even number and an odd number of arms to have. The only number both odd and even is infinity. Stealing shamelessly from the journal of Irreproducible results I've exhausted my ideas.
Frank
-----Original Message----- X-from: bigelow-at-umich.edu [mailto:bigelow-at-umich.edu] Sent: Wednesday, October 12, 2011 1:34 PM To: Frank Karl
Listers:
This is an interesting question and has generated a bunch of interesting answers. Traditionally, the 10-x point would be interpreted as the upper end of the 10-x range, so that on pumping down, when you pass below the 10-x point you are into the 10-x range. On this basis the reading shown would be 2 x 10-5 Torr.
However, just to get an authoritative answer I have forwarded Joim's original message to info-at-dentonvacuum.com. Let's see what response we get. -- Wilbur C. Bigelow, Professor Emeritus Materials Sci. & Engr., Univ. of Michigan Ann Arbor, Michigan 48109-2136 e-mail: bigelow-at-umich.edu; Fx:734-763-4788; Ph:734-975-0858 Address mail to: 2911 Whittier Court Ann Arbor, MI 48104-6731
==============================Original Headers============================== 3, 15 -- From bigelow-at-umich.edu Wed Oct 12 12:26:41 2011 3, 15 -- Received: from hackers.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.81]) 3, 15 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CHQfnN029690 3, 15 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:26:41 -0500 3, 15 -- Received: FROM [76.226.9.147] (adsl-76-226-9-147.dsl.sfldmi.sbcglobal.net [76.226.9.147]) 3, 15 -- By hackers.mr.itd.umich.edu ID 4E95CDD0.68275.25452 ; 3, 15 -- Authuser bigelow; 3, 15 -- 12 Oct 2011 13:26:40 EDT 3, 15 -- Mime-Version: 1.0 3, 15 -- Message-Id: {p06240802cabb7cd686d6-at-[76.226.9.147]} 3, 15 -- Date: Wed, 12 Oct 2011 13:26:24 -0400 3, 15 -- To: Microscopy Listserver {microscopy-at-microscopy.com} 3, 15 -- From: Wil Bigelow {bigelow-at-umich.edu} 3, 15 -- Subject: [Microscopy]RE; Vac Gauge reading 3, 15 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 18, 26 -- From frank_karl-at-ardl.com Wed Oct 12 13:07:46 2011 18, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 18, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CI7jDx031952 18, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:07:46 -0500 18, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 18, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Wed, 12 18, 26 -- Oct 2011 14:07:45 -0400 18, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 18, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 18, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 18, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 18, 26 -- Date: Wed, 12 Oct 2011 14:07:44 -0400 18, 26 -- Subject: RE: [Microscopy] RE; Vac Gauge reading 18, 26 -- Thread-Topic: [Microscopy] RE; Vac Gauge reading 18, 26 -- Thread-Index: AcyJBTHtYGR/8HVQSzq7835K8t3RYgAAEPjw 18, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077DA35AFBF-at-exchange2k7.ad.ardl.com} 18, 26 -- In-Reply-To: {201110121734.p9CHYJHl012989-at-ns.microscopy.com} 18, 26 -- Accept-Language: en-US 18, 26 -- Content-Language: en-US 18, 26 -- X-MS-Has-Attach: 18, 26 -- X-MS-TNEF-Correlator: 18, 26 -- acceptlanguage: en-US 18, 26 -- Content-Type: text/plain; charset="iso-8859-1" 18, 26 -- MIME-Version: 1.0 18, 26 -- Content-Transfer-Encoding: 8bit 18, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CI7jDx031952 ==============================End of - Headers==============================
Sorry, I just can't resist. From the movie 'Aliens':
Vasquez: They're right on us. Hicks: [waiting for the Aliens]: Remember, short controlled bursts. Hudson: 9 meters. 7. 6. Ripley: That can't be; that's inside the room. Hudson: It's reading right man, look! Hicks: Then you're not reading *it* right. Hudson: 5 meters, man 4. What the hell?
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Wow, I've always read these a certain way, but now I see where there might be some confusion.
On my equipment I've put little arrows with labels: pointing right = "worse" and pointing left = "better" to show which way the gauges go. So I see 2x10-5.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 22 -- From tina-at-pbrc.hawaii.edu Wed Oct 12 13:23:30 2011 4, 22 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CINTZ4018449 4, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:23:30 -0500 4, 22 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id p9CINSEU000689 4, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 08:23:28 -1000 (HST) 4, 22 -- Received: from localhost (tina-at-localhost) 4, 22 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id p9CINR1A000685 4, 22 -- for {Microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 08:23:28 -1000 (HST) 4, 22 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 22 -- Date: Wed, 12 Oct 2011 08:23:27 -1000 (HST) 4, 22 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 22 -- X-X-Sender: tina-at-b1000 4, 22 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 22 -- Subject: Re: [Microscopy] vacuum gauge reading (so far) 4, 22 -- In-Reply-To: {201110121617.p9CGHXx5011511-at-ns.microscopy.com} 4, 22 -- Message-ID: {Pine.GSO.4.64.1110120820220.620-at-b1000} 4, 22 -- References: {201110121617.p9CGHXx5011511-at-ns.microscopy.com} 4, 22 -- MIME-Version: 1.0 4, 22 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
I admit to being a little blindsided by Jim's original posting, but I hope no harm was done by my erring on the side of "didaction for dummies". Excuse me if I did. My excuse: I am accustomed to educating graduate students.
Jim, if your sense is that the time of pumping was insufficient to reach 10e-6 range, therefore the meter MUST be displaying 2 x 10e-5, perhaps there is an issue of inaccurate, uncalibrated, or otherwise deficient gauges. (Or a really fast pump.) Most thermocouple and ionizing gauges will become inaccurate through buildup of surface deposits on the gauge. Also they are calibrated for specific gases; a different gas composition will result in an inaccurate gauge.
All my money is still on 2 x 10e-6. HOWEVER, one caveat: If we get word from the maker of the meter and they meant 10e-6 = 10 x 10e-6, then I withdraw my wager. But I would still insist that is an improper way to label the meter.
Still cheerful Roger
------ Forwarded Message X-from: Roger Ristau {raristau-at-ims.uconn.edu}
This has been a fun thread to follow....
With all humility and respect, it is plain that Jim is not the only one confused by these gauges. I have stared at a meter for many minutes many times as I tried to sort this out in my own head.
Vacuum gauges, whether measuring current, voltage, resistance, etc. are just like most other meters: The lower, or smaller, or least magnitude values are on the left, the larger on the right. It is just that the pressure gauge starts with the needle pointing to the larger value on the right (closer to atmospheric pressure), and moves to the smaller value end of the meter on the left as the pumps improve the vacuum. We are more accustomed to most other sorts of gauges in which the needle starts by pointing to the smaller magnitude on the left and moves left to right as the gadget it is attached to does its stuff.
Therefore, the vacuum meter reading, as the needle moves to the left of the tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the larger magnitude to the right of the smaller magnitude. The meter in Jim's image is reading just a hair higher than 2 x 10e-6.
Of course, not content to leave well enough alone, we create most of our own confusion when we refer to "higher vacuum" as the state when the magnitude of pressure is "lower", and "lower vacuum" when the pressure reading is of "higher" magnitude. But that is another thread...
With smiles Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CGopcD011522 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 (EDT) 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 9, 21 -- Subject: Vacuum gauge reading 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} 9, 21 -- Thread-Topic: Vacuum gauge reading 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== 9, 21 -- Mime-version: 1.0 9, 21 -- Content-type: text/plain; 9, 21 -- charset="US-ASCII" 9, 21 -- Content-transfer-encoding: 7bit 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 9, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
------ End of Forwarded Message
------ End of Forwarded Message
==============================Original Headers============================== 28, 22 -- From raristau-at-ims.uconn.edu Wed Oct 12 13:29:03 2011 28, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CIT3Qf004544 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:29:03 -0500 28, 22 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 28, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id DFD4C2CFCA 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:29:02 -0400 (EDT) 28, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge reading 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge reading 28, 22 -- Thread-Index: AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} 28, 22 -- Mime-version: 1.0 28, 22 -- Content-type: text/plain; 28, 22 -- charset="US-ASCII" 28, 22 -- Content-transfer-encoding: 7bit 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 28, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Roger just said everything I was going to say. If Denton did this correctly, the gauge is reading 2x10E-6 (slightly higher pressure than 1x10E-6 to its left). If they screwed up the calibration, shame on them. Being a cold cathode gauge it may be in need of both cleaning and calibrating.
In my experience if the 1x10E-5 was to indicate the range (as on some Varian gauges), then it would have been placed roughly over the 4 in the range that was intended. If it is placed on the end of a range, it indicates 1x10E-?
My $.02
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] Sent: Wednesday, October 12, 2011 2:32 PM To: kenconverse-at-qualityimages.biz
OK here comes Roger v1.3...
I admit to being a little blindsided by Jim's original posting, but I hope no harm was done by my erring on the side of "didaction for dummies". Excuse me if I did. My excuse: I am accustomed to educating graduate students.
Jim, if your sense is that the time of pumping was insufficient to reach 10e-6 range, therefore the meter MUST be displaying 2 x 10e-5, perhaps there is an issue of inaccurate, uncalibrated, or otherwise deficient gauges. (Or a really fast pump.) Most thermocouple and ionizing gauges will become inaccurate through buildup of surface deposits on the gauge. Also they are calibrated for specific gases; a different gas composition will result in an inaccurate gauge.
All my money is still on 2 x 10e-6. HOWEVER, one caveat: If we get word from the maker of the meter and they meant 10e-6 = 10 x 10e-6, then I withdraw my wager. But I would still insist that is an improper way to label the meter.
Still cheerful Roger
------ Forwarded Message X-from: Roger Ristau {raristau-at-ims.uconn.edu}
This has been a fun thread to follow....
With all humility and respect, it is plain that Jim is not the only one confused by these gauges. I have stared at a meter for many minutes many times as I tried to sort this out in my own head.
Vacuum gauges, whether measuring current, voltage, resistance, etc. are just like most other meters: The lower, or smaller, or least magnitude values are on the left, the larger on the right. It is just that the pressure gauge starts with the needle pointing to the larger value on the right (closer to atmospheric pressure), and moves to the smaller value end of the meter on the left as the pumps improve the vacuum. We are more accustomed to most other sorts of gauges in which the needle starts by pointing to the smaller magnitude on the left and moves left to right as the gadget it is attached to does its stuff.
Therefore, the vacuum meter reading, as the needle moves to the left of the tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the larger magnitude to the right of the smaller magnitude. The meter in Jim's image is reading just a hair higher than 2 x 10e-6.
Of course, not content to leave well enough alone, we create most of our own confusion when we refer to "higher vacuum" as the state when the magnitude of pressure is "lower", and "lower vacuum" when the pressure reading is of "higher" magnitude. But that is another thread...
With smiles Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CGopcD011522 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 (EDT) 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 9, 21 -- Subject: Vacuum gauge reading 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} 9, 21 -- Thread-Topic: Vacuum gauge reading 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== 9, 21 -- Mime-version: 1.0 9, 21 -- Content-type: text/plain; 9, 21 -- charset="US-ASCII" 9, 21 -- Content-transfer-encoding: 7bit 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 9, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
------ End of Forwarded Message
------ End of Forwarded Message
==============================Original Headers============================== 28, 22 -- From raristau-at-ims.uconn.edu Wed Oct 12 13:29:03 2011 28, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CIT3Qf004544 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:29:03 -0500 28, 22 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 28, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id DFD4C2CFCA 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:29:02 -0400 (EDT) 28, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge reading 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge reading 28, 22 -- Thread-Index: AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} 28, 22 -- Mime-version: 1.0 28, 22 -- Content-type: text/plain; 28, 22 -- charset="US-ASCII" 28, 22 -- Content-transfer-encoding: 7bit 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 28, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 40, 28 -- From kenconverse-at-qualityimages.biz Wed Oct 12 14:12:27 2011 40, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 40, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CJCRaS002197 40, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:12:27 -0500 40, 28 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW c=1 sm=0 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=a28-TuC6_4MA:10 a=kj9zAlcOel0A:10 a=Zx37jsudAAAA:8 a=MKxSoT7or7n8rsCZ3E0A:9 a=MjCJU8d-ybvQtNNfEm4A:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=ZC8Yv9kwYYWqhca4:21 a=CTP1pKWpgRALcfUy:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 40, 28 -- X-Cloudmark-Score: 0 40, 28 -- X-Originating-IP: 72.224.149.57 40, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2423] helo=Ken) 40, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 40, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 40, 28 -- id C3/0A-06600-A96E59E4; Wed, 12 Oct 2011 19:12:27 +0000 40, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 40, 28 -- To: {raristau-at-ims.uconn.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 40, 28 -- Subject: RE: [Microscopy] FW: Vacuum gauge reading 40, 28 -- Date: Wed, 12 Oct 2011 15:12:20 -0400 40, 28 -- Message-ID: {656CA10E51FE480792DFFCCF028FA6B3-at-Ken} 40, 28 -- MIME-Version: 1.0 40, 28 -- Content-Type: text/plain; 40, 28 -- charset="us-ascii" 40, 28 -- X-Priority: 3 (Normal) 40, 28 -- X-MSMail-Priority: Normal 40, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 40, 28 -- In-Reply-To: {201110121831.p9CIVpWw015451-at-ns.microscopy.com} 40, 28 -- Importance: Normal 40, 28 -- Thread-Index: AcyJDTQvEHHpw1XfTzyWCbC6rI/RigABLkiw 40, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 40, 28 -- Content-Transfer-Encoding: 8bit 40, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CJCRaS002197 ==============================End of - Headers==============================
I can hardly believe that this is even debateable!
The gauge measures pressure (even though sub-atmospheric pressures are also called "vacuums").
Higher pressures are to the RHS, thus a pressure of 10exp-3 is a higher pressure than one of 10exp-9.
So, as the pressure decreases from 10exp-5, it goes: 1x10exp-5, 0.8x10exp-5 (ie 8x10exp-6), 0.6x10exp-5 (ie 6x10exp-6) etc etc.
I can't see that it is indicating anything but a smidgen above (pressure!) 0.2x10exp-5, ie 2x10exp-6, but maybe I'm just not seeing the problem clearly.
cheers Ritchie Sims University of Auckland
________________________________________ X-from: kenconverse-at-qualityimages.biz [kenconverse-at-qualityimages.biz] Sent: 13 October 2011 08:13 To: Ritchie Sims
Roger just said everything I was going to say. If Denton did this correctly, the gauge is reading 2x10E-6 (slightly higher pressure than 1x10E-6 to its left). If they screwed up the calibration, shame on them. Being a cold cathode gauge it may be in need of both cleaning and calibrating.
In my experience if the 1x10E-5 was to indicate the range (as on some Varian gauges), then it would have been placed roughly over the 4 in the range that was intended. If it is placed on the end of a range, it indicates 1x10E-?
My $.02
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] Sent: Wednesday, October 12, 2011 2:32 PM To: kenconverse-at-qualityimages.biz
OK here comes Roger v1.3...
I admit to being a little blindsided by Jim's original posting, but I hope no harm was done by my erring on the side of "didaction for dummies". Excuse me if I did. My excuse: I am accustomed to educating graduate students.
Jim, if your sense is that the time of pumping was insufficient to reach 10e-6 range, therefore the meter MUST be displaying 2 x 10e-5, perhaps there is an issue of inaccurate, uncalibrated, or otherwise deficient gauges. (Or a really fast pump.) Most thermocouple and ionizing gauges will become inaccurate through buildup of surface deposits on the gauge. Also they are calibrated for specific gases; a different gas composition will result in an inaccurate gauge.
All my money is still on 2 x 10e-6. HOWEVER, one caveat: If we get word from the maker of the meter and they meant 10e-6 = 10 x 10e-6, then I withdraw my wager. But I would still insist that is an improper way to label the meter.
Still cheerful Roger
------ Forwarded Message X-from: Roger Ristau {raristau-at-ims.uconn.edu}
This has been a fun thread to follow....
With all humility and respect, it is plain that Jim is not the only one confused by these gauges. I have stared at a meter for many minutes many times as I tried to sort this out in my own head.
Vacuum gauges, whether measuring current, voltage, resistance, etc. are just like most other meters: The lower, or smaller, or least magnitude values are on the left, the larger on the right. It is just that the pressure gauge starts with the needle pointing to the larger value on the right (closer to atmospheric pressure), and moves to the smaller value end of the meter on the left as the pumps improve the vacuum. We are more accustomed to most other sorts of gauges in which the needle starts by pointing to the smaller magnitude on the left and moves left to right as the gadget it is attached to does its stuff.
Therefore, the vacuum meter reading, as the needle moves to the left of the tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the larger magnitude to the right of the smaller magnitude. The meter in Jim's image is reading just a hair higher than 2 x 10e-6.
Of course, not content to leave well enough alone, we create most of our own confusion when we refer to "higher vacuum" as the state when the magnitude of pressure is "lower", and "lower vacuum" when the pressure reading is of "higher" magnitude. But that is another thread...
With smiles Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
==============================Original Headers============================== 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CGopcD011522 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 (EDT) 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 9, 21 -- Subject: Vacuum gauge reading 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} 9, 21 -- Thread-Topic: Vacuum gauge reading 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== 9, 21 -- Mime-version: 1.0 9, 21 -- Content-type: text/plain; 9, 21 -- charset="US-ASCII" 9, 21 -- Content-transfer-encoding: 7bit 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 9, 21 -- X-Virus-Status: Clean ==============================End of - Headers==============================
------ End of Forwarded Message
------ End of Forwarded Message
==============================Original Headers============================== 28, 22 -- From raristau-at-ims.uconn.edu Wed Oct 12 13:29:03 2011 28, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu [137.99.25.235]) 28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CIT3Qf004544 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:29:03 -0500 28, 22 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 28, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id DFD4C2CFCA 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:29:02 -0400 (EDT) 28, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge reading 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge reading 28, 22 -- Thread-Index: AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} 28, 22 -- Mime-version: 1.0 28, 22 -- Content-type: text/plain; 28, 22 -- charset="US-ASCII" 28, 22 -- Content-transfer-encoding: 7bit 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 28, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
==============================Original Headers============================== 40, 28 -- From kenconverse-at-qualityimages.biz Wed Oct 12 14:12:27 2011 40, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 40, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CJCRaS002197 40, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:12:27 -0500 40, 28 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW c=1 sm=0 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=a28-TuC6_4MA:10 a=kj9zAlcOel0A:10 a=Zx37jsudAAAA:8 a=MKxSoT7or7n8rsCZ3E0A:9 a=MjCJU8d-ybvQtNNfEm4A:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=ZC8Yv9kwYYWqhca4:21 a=CTP1pKWpgRALcfUy:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 40, 28 -- X-Cloudmark-Score: 0 40, 28 -- X-Originating-IP: 72.224.149.57 40, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2423] helo=Ken) 40, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 40, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 40, 28 -- id C3/0A-06600-A96E59E4; Wed, 12 Oct 2011 19:12:27 +0000 40, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 40, 28 -- To: {raristau-at-ims.uconn.edu} , "MSA Listserver" {microscopy-at-microscopy.com} 40, 28 -- Subject: RE: [Microscopy] FW: Vacuum gauge reading 40, 28 -- Date: Wed, 12 Oct 2011 15:12:20 -0400 40, 28 -- Message-ID: {656CA10E51FE480792DFFCCF028FA6B3-at-Ken} 40, 28 -- MIME-Version: 1.0 40, 28 -- Content-Type: text/plain; 40, 28 -- charset="us-ascii" 40, 28 -- X-Priority: 3 (Normal) 40, 28 -- X-MSMail-Priority: Normal 40, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 40, 28 -- In-Reply-To: {201110121831.p9CIVpWw015451-at-ns.microscopy.com} 40, 28 -- Importance: Normal 40, 28 -- Thread-Index: AcyJDTQvEHHpw1XfTzyWCbC6rI/RigABLkiw 40, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 40, 28 -- Content-Transfer-Encoding: 8bit 40, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CJCRaS002197 ==============================End of - Headers==============================
Like Ritchie, I am surprised at the multitude of responses and rationales for reading the pressure. The pressure reading is obvious just by looking at the scale. You don't need to think about which way the gauge moves or how things pump down. These are just red herrings!
The gauge is a PRESSURE gauge. It has an obvious logarithmic scale with higher pressures to the right. The numbers between 10-6 and 10-5 are the pressures between 1x10-6 and 1x10-5 (= 10x10-6).
The pressure is approx. 2x10-6 (or to coin a unit... 2 microtorr )
{/ on soapbox/} There is no doubt (and should be a very short discussion!) {/off soapbox/}
Cheers, Henk
At 10/12/2011 4:38 PM, r.sims-at-auckland.ac.nz wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I can hardly believe that this is even debateable! } } The gauge measures pressure (even though sub-atmospheric pressures are also called "vacuums"). } } Higher pressures are to the RHS, thus a pressure of 10exp-3 is a higher pressure than one of 10exp-9. } } So, as the pressure decreases from 10exp-5, it goes: 1x10exp-5, 0.8x10exp-5 (ie 8x10exp-6), 0.6x10exp-5 (ie 6x10exp-6) etc etc. } } I can't see that it is indicating anything but a smidgen above (pressure!) 0.2x10exp-5, ie 2x10exp-6, but maybe I'm just not seeing the problem clearly. } } cheers } Ritchie Sims } University of Auckland } } } } } ________________________________________ } X-from: kenconverse-at-qualityimages.biz [kenconverse-at-qualityimages.biz] } Sent: 13 October 2011 08:13 } To: Ritchie Sims } Subject: [Microscopy] RE: FW: Vacuum gauge reading } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Roger just said everything I was going to say. If Denton did this } correctly, the gauge is reading 2x10E-6 (slightly higher pressure than } 1x10E-6 to its left). If they screwed up the calibration, shame on them. } Being a cold cathode gauge it may be in need of both cleaning and } calibrating. } } In my experience if the 1x10E-5 was to indicate the range (as on some Varian } gauges), then it would have been placed roughly over the 4 in the range that } was intended. If it is placed on the end of a range, it indicates 1x10E-? } } My $.02 } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] } Sent: Wednesday, October 12, 2011 2:32 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] FW: Vacuum gauge reading } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } OK here comes Roger v1.3... } } I admit to being a little blindsided by Jim's original posting, but I hope } no harm was done by my erring on the side of "didaction for dummies". Excuse } me if I did. My excuse: I am accustomed to educating graduate students. } } Jim, if your sense is that the time of pumping was insufficient to reach } 10e-6 range, therefore the meter MUST be displaying 2 x 10e-5, perhaps there } is an issue of inaccurate, uncalibrated, or otherwise deficient gauges. (Or } a really fast pump.) Most thermocouple and ionizing gauges will become } inaccurate through buildup of surface deposits on the gauge. Also they are } calibrated for specific gases; a different gas composition will result in an } inaccurate gauge. } } All my money is still on 2 x 10e-6. HOWEVER, one caveat: If we get word from } the maker of the meter and they meant 10e-6 = 10 x 10e-6, then I withdraw my } wager. But I would still insist that is an improper way to label the meter. } } Still cheerful } Roger } } } ------ Forwarded Message } X-from: Roger Ristau {raristau-at-ims.uconn.edu} } Date: Wed, 12 Oct 2011 13:48:48 -0400 } To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } Conversation: [Microscopy] Vacuum gauge reading } Subject: FW: [Microscopy] Vacuum gauge reading } } A follow-up on my previous message: (I can't get enough "out-of-office" } replies!) } } A tic marked 10e-6 means 1 x 10e-6. } It makes no sense for it to represent 10 x 10e-6; that is an "improper } fraction". } } Cheers all } Roger } } ------ Forwarded Message } X-from: {raristau-at-ims.uconn.edu} } Reply-To: {raristau-at-ims.uconn.edu} } Date: Wed, 12 Oct 2011 11:53:12 -0500 } To: {raristau-at-ims.uconn.edu} } Subject: [Microscopy] Vacuum gauge reading } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This has been a fun thread to follow.... } } With all humility and respect, it is plain that Jim is not the only one } confused by these gauges. I have stared at a meter for many minutes many } times as I tried to sort this out in my own head. } } Vacuum gauges, whether measuring current, voltage, resistance, etc. are just } like most other meters: The lower, or smaller, or least magnitude values are } on the left, the larger on the right. It is just that the pressure gauge } starts with the needle pointing to the larger value on the right (closer to } atmospheric pressure), and moves to the smaller value end of the meter on } the left as the pumps improve the vacuum. We are more accustomed to most } other sorts of gauges in which the needle starts by pointing to the smaller } magnitude on the left and moves left to right as the gadget it is attached } to does its stuff. } } Therefore, the vacuum meter reading, as the needle moves to the left of the } tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note } that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the } larger magnitude to the right of the smaller magnitude. The meter in Jim's } image is reading just a hair higher than 2 x 10e-6. } } Of course, not content to leave well enough alone, we create most of our own } confusion when we refer to "higher vacuum" as the state when the magnitude } of pressure is "lower", and "lower vacuum" when the pressure reading is of } "higher" magnitude. But that is another thread... } } With smiles } Roger } } Roger A. Ristau, PhD } Electron Microscopy Specialist } Institute of Materials Science } 97 North Eagleville Road } University of Connecticut } Storrs, CT 06269 } vox: 860-486-5453 } fax: 860-486-4745 } } } } } ==============================Original Headers============================== } 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 } 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu } [137.99.25.235]) } 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p9CGopcD011522 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 } 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu } [137.99.20.184]) } 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 } (EDT) } 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 } 9, 21 -- Subject: Vacuum gauge reading } 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} } 9, 21 -- Thread-Topic: Vacuum gauge reading } 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== } 9, 21 -- Mime-version: 1.0 } 9, 21 -- Content-type: text/plain; } 9, 21 -- charset="US-ASCII" } 9, 21 -- Content-transfer-encoding: 7bit } 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 } 9, 21 -- X-Virus-Status: Clean } ==============================End of - Headers============================== } } ------ End of Forwarded Message } } ------ End of Forwarded Message } } } } ==============================Original Headers============================== } 28, 22 -- From raristau-at-ims.uconn.edu Wed Oct 12 13:29:03 2011 } 28, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu } [137.99.25.235]) } 28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p9CIT3Qf004544 } 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:29:03 } -0500 } 28, 22 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu } [137.99.20.184]) } 28, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id DFD4C2CFCA } 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:29:02 } -0400 (EDT) } 28, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 } 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge reading } 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} } 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge reading } 28, 22 -- Thread-Index: AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK } 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} } 28, 22 -- Mime-version: 1.0 } 28, 22 -- Content-type: text/plain; } 28, 22 -- charset="US-ASCII" } 28, 22 -- Content-transfer-encoding: 7bit } 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 } 28, 22 -- X-Virus-Status: Clean } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 40, 28 -- From kenconverse-at-qualityimages.biz Wed Oct 12 14:12:27 2011 } 40, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) } 40, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CJCRaS002197 } 40, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:12:27 -0500 } 40, 28 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW c=1 sm=0 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=a28-TuC6_4MA:10 a=kj9zAlcOel0A:10 a=Zx37jsudAAAA:8 a=MKxSoT7or7n8rsCZ3E0A:9 a=MjCJU8d-ybvQtNNfEm4A:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=ZC8Yv9kwYYWqhca4:21 a=CTP1pKWpgRALcfUy:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 40, 28 -- X-Cloudmark-Score: 0 } 40, 28 -- X-Originating-IP: 72.224.149.57 } 40, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2423] helo=Ken) } 40, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) } 40, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP } 40, 28 -- id C3/0A-06600-A96E59E4; Wed, 12 Oct 2011 19:12:27 +0000 } 40, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 40, 28 -- To: {raristau-at-ims.uconn.edu} , "MSA Listserver" {microscopy-at-microscopy.com} } 40, 28 -- Subject: RE: [Microscopy] FW: Vacuum gauge reading } 40, 28 -- Date: Wed, 12 Oct 2011 15:12:20 -0400 } 40, 28 -- Message-ID: {656CA10E51FE480792DFFCCF028FA6B3-at-Ken} } 40, 28 -- MIME-Version: 1.0 } 40, 28 -- Content-Type: text/plain; } 40, 28 -- charset="us-ascii" } 40, 28 -- X-Priority: 3 (Normal) } 40, 28 -- X-MSMail-Priority: Normal } 40, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 40, 28 -- In-Reply-To: {201110121831.p9CIVpWw015451-at-ns.microscopy.com} } 40, 28 -- Importance: Normal } 40, 28 -- Thread-Index: AcyJDTQvEHHpw1XfTzyWCbC6rI/RigABLkiw } 40, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 } 40, 28 -- Content-Transfer-Encoding: 8bit } 40, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CJCRaS002197 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 57, 51 -- From r.sims-at-auckland.ac.nz Wed Oct 12 15:37:14 2011 } 57, 51 -- Received: from mx1.auckland.ac.nz (mx1.auckland.ac.nz [130.216.12.42]) } 57, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CKbA9k020103 } 57, 51 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 15:37:13 -0500 } 57, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 57, 51 -- d=auckland.ac.nz; i=r.sims-at-auckland.ac.nz; q=dns/txt; } 57, 51 -- s=uoa; t=1318451833; x=1349987833; } 57, 51 -- h=from:to:subject:date:message-id:references:in-reply-to: } 57, 51 -- content-transfer-encoding:mime-version; } 57, 51 -- z=From:=20Ritchie=20Sims=20 {r.sims-at-auckland.ac.nz} |To:=20" } 57, 51 -- microscopy-at-microscopy.com"=20 {microscopy-at-microscopy.com} } 57, 51 -- |Subject:=20RE:=20[Microscopy]=20RE:=20FW:=20Vacuum=20gau } 57, 51 -- ge=20reading|Date:=20Wed,=2012=20Oct=202011=2020:37:08=20 } 57, 51 -- +0000|Message-ID:=20 {65F9DBC3DFB6DE44BE7695B82D539F1A1E2E } 57, 51 -- 5466-at-uxcn10-1.UoA.auckland.ac.nz} |References:=20 {20111012 } 57, 51 -- 1913.p9CJDAAn003326-at-ns.microscopy.com} |In-Reply-To:=20 {20 } 57, 51 -- 1110121913.p9CJDAAn003326-at-ns.microscopy.com} } 57, 51 -- |Content-Transfer-Encoding:=20quoted-printable } 57, 51 -- |MIME-Version:=201.0; } 57, 51 -- bh=LehGCDvCVGAn953JQ2RBlkT0GfSh8/8XdcjaxaFFNCI=; } 57, 51 -- b=SbYW6y40G6L7sqBgt71/dAfEArbKeaqB9x0t7NqNYLinq7SXflF4VRxT } 57, 51 -- aerEib3VGlMmGFJt/DriIM0ea0to68GeWzdTnnlNNWEdDCw7pO443vNQ1 } 57, 51 -- kTxLBJTmhNCHqa6dLp1rXkSwK7jVc4iWxT2NDQz+gjyHyjjWil2jRsi9O } 57, 51 -- k=; } 57, 51 -- X-IronPort-AV: E=Sophos;i="4.69,336,1315137600"; } 57, 51 -- d="scan'208";a="106294466" } 57, 51 -- X-Ironport-HAT: MAIL-SERVERS - $RELAYED } 57, 51 -- X-Ironport-Source: 130.216.4.106 - Outgoing - Outgoing } 57, 51 -- Received: from uxchange10-fe2.itsys.auckland.ac.nz (HELO uxchange10-fe2.UoA.auckland.ac.nz) ([130.216.4.106]) } 57, 51 -- by mx1-int.auckland.ac.nz with ESMTP/TLS/AES128-SHA; 13 Oct 2011 09:37:08 +1300 } 57, 51 -- Received: from UXCN10-1.UoA.auckland.ac.nz ([169.254.1.62]) by } 57, 51 -- uxchange10-fe2.UoA.auckland.ac.nz ([130.216.4.106]) with mapi id } 57, 51 -- 14.01.0289.001; Thu, 13 Oct 2011 09:37:08 +1300 } 57, 51 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz} } 57, 51 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 57, 51 -- Subject: RE: [Microscopy] RE: FW: Vacuum gauge reading } 57, 51 -- Thread-Topic: [Microscopy] RE: FW: Vacuum gauge reading } 57, 51 -- Thread-Index: AQHMiRL9WURKj4XdpkSfOkAwP4r5CJV5Jz5f } 57, 51 -- Date: Wed, 12 Oct 2011 20:37:08 +0000 } 57, 51 -- Message-ID: {65F9DBC3DFB6DE44BE7695B82D539F1A1E2E5466-at-uxcn10-1.UoA.auckland.ac.nz} } 57, 51 -- References: {201110121913.p9CJDAAn003326-at-ns.microscopy.com} } 57, 51 -- In-Reply-To: {201110121913.p9CJDAAn003326-at-ns.microscopy.com} } 57, 51 -- Accept-Language: en-GB, en-NZ, en-US } 57, 51 -- Content-Language: en-GB } 57, 51 -- X-MS-Has-Attach: } 57, 51 -- X-MS-TNEF-Correlator: } 57, 51 -- x-originating-ip: [130.216.11.103] } 57, 51 -- Content-Type: text/plain; charset="us-ascii" } 57, 51 -- MIME-Version: 1.0 } 57, 51 -- Content-Transfer-Encoding: 8bit } 57, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CKbA9k020103 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 10, 26 -- From colijn.1-at-osu.edu Wed Oct 12 15:59:37 2011 10, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CKxbDC004509 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 15:59:37 -0500 10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 26 -- id {01O74Y6Y2DA892XO6X-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 10, 26 -- Wed, 12 Oct 2011 16:59:35 -0400 (EDT) 10, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 10, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 26 -- with ESMTPA id {01O74Y6XKIP493YOD3-at-ecr6.ohio-state.edu} ; Wed, 10, 26 -- 12 Oct 2011 16:59:34 -0400 (EDT) 10, 26 -- Date: Wed, 12 Oct 2011 16:59:34 -0400 10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 26 -- Subject: Re: [Microscopy] FW: Vacuum gauge reading 10, 26 -- In-reply-to: {201110122038.p9CKcUgZ021962-at-ns.microscopy.com} 10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 26 -- To: r.sims-at-auckland.ac.nz, microscopy-at-microscopy.com 10, 26 -- Message-id: {4E95FFB6.5050900-at-osu.edu} 10, 26 -- MIME-version: 1.0 10, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 10, 26 -- Content-transfer-encoding: 7bit 10, 26 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 10, 26 -- Thunderbird/7.0.1 10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 26 -- References: {201110122038.p9CKcUgZ021962-at-ns.microscopy.com} ==============================End of - Headers==============================
Hi Jim, Take a look at http://www2.snapfish.com/snapfish/thumbnailshare/AlbumID=3851367021/a=162307 140_162307140/otsc=SHR/otsi=SALBlink/COBRAND_NAME=snapfish/ These two meters are both on Varian Smart Gauges and I think they illustrate where some of the confusion is coming from. One indicates where 1x10E-* is located while the other indicates where the 10E-* range (or decade) is located. Both meters cover the identical range and can only be read correctly one way.
Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: James Ehrman [mailto:jehrman-at-mta.ca] Sent: Wednesday, October 12, 2011 3:19 PM To: kenconverse-at-qualityimages.biz
Ken's images are the clearest possible indication about where the ranges are:
On 10/12/2011 5:13 PM, kenconverse-at-qualityimages.biz wrote: } http://www2.snapfish.com/snapfish/thumbnailshare/AlbumID=3851367021/a=162307 } 140_162307140/otsc=SHR/otsi=SALBlink/COBRAND_NAME=snapfish/
10% Nitrogen lines up with 1 microTorr (not sure what that's all about) but on both gauges it corresponds to the same.
And 2E-6 mb is very nice for an evaporator ten minutes after pump-down (without LN), perhaps it has a turbo-pump?
Interesting discussion all the same, thanks Rob Keyse Lehigh EM facility.
==============================Original Headers============================== 5, 22 -- From rok210-at-lehigh.edu Wed Oct 12 16:22:58 2011 5, 22 -- Received: from rain.cc.lehigh.edu (rain.cc.lehigh.edu [128.180.2.160]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CLMwiQ005656 5, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 16:22:58 -0500 5, 22 -- Received: from [128.180.55.15] (Dyn055015.mat.Lehigh.EDU [128.180.55.15]) 5, 22 -- (authenticated bits=0) 5, 22 -- by rain.cc.lehigh.edu (8.14.4/8.14.5) with ESMTP id p9CLMwA6011810 5, 22 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 5, 22 -- Wed, 12 Oct 2011 17:22:58 -0400 5, 22 -- Message-ID: {4E960531.1070000-at-lehigh.edu} 5, 22 -- Date: Wed, 12 Oct 2011 17:22:57 -0400 5, 22 -- From: Robert Keyse {rok210-at-lehigh.edu} 5, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:7.0) Gecko/20110922 Thunderbird/7.0 5, 22 -- MIME-Version: 1.0 5, 22 -- To: kenconverse-at-qualityimages.biz, microscopy-at-microscopy.com 5, 22 -- Subject: Re: [Microscopy] FW: Vacuum gauge reading 5, 22 -- References: {201110122113.p9CLDc2C003257-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201110122113.p9CLDc2C003257-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-Virus-Scanned: clamav-milter 0.97.2 at rain.cc.lehigh.edu 5, 22 -- X-Virus-Status: Clean ==============================End of - Headers==============================
I've been enjoying reading the flow of emails all day.
Could you tell us what your vacuum "chamber" is composed of material wise? Are you dealing with a large glass bell jar and a Viton or Buna-N gasket? Is the reading on your gauge typical of your ultimate pressure (without Liq Nitrogen)?
The reason I ask is that if you do have a large glass bell jar with a Viton gasket that has not been baked out in some time, then you should be hitting a double wall in the pump down. Your hitting both the water desorption issue from the inside surfaces of your "chamber" as well as the bottoming out of the typical pressure you can normally get with a non-baked system with a Viton vacuum seal without a LiqNitrogen cold finger - something in the low e-6 torr range. If you are in fact in the e-5 range then you probably have a small leak!
I'm firmly in the camp that your gauge reads 2e-6 torr!
Mike
******************************************************************** Michael M. Cheatham 321 Heroy Geology Laboratory Phone (315)-443-1261 Syracuse University Fax (315)-443-3363 Syracuse, NY 13244-1070
owner of PLASMACHEM-L: http://listserv.syr.edu/archives/plasmachem-l.html owner of XRF-L: http://listserv.syr.edu/archives/xrf-l.html owner of TIMS-L: http://listserv.syr.edu/archives/tims-l.html
Justin, Does this work? http://tinyurl.com/vacuum-meter Thanks for the suggestion Ken
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] Sent: Wednesday, October 12, 2011 5:09 PM To: kenconverse-at-qualityimages.biz
If this reads 10-5, the first set of coefficients on the right side of the gauge has no exponent associated with it, and the last exponent (10-9) has no coefficients.
If it reads 10-6, then each exponent has a set of coefficients and vice versa.
Kleo
--- On Wed, 10/12/11, Kleo Pullin {kleopullin-at-pacbell.net} wrote:
} From: Kleo Pullin {kleopullin-at-pacbell.net} } Subject: Re: [Microscopy] RE: FW: Vacuum gauge reading } To: KLeoPullin-at-pacbell.net, kenconverse-at-qualityimages.biz } Date: Wednesday, October 12, 2011, 4:22 PM } If this reads 10-5, the first set of } coefficients on the right side of the gauge has no exponent } associated with it, and the last exponent (10-9) has no } coefficients. } } If it reads 10-6, then each exponent has a set of } coefficients and vice versa. } } Kleo } } --- On Wed, 10/12/11, kenconverse-at-qualityimages.biz } {kenconverse-at-qualityimages.biz} } wrote: } } From: kenconverse-at-qualityimages.biz } {kenconverse-at-qualityimages.biz} } Subject: [Microscopy] RE: FW: Vacuum gauge reading } To: KLeoPullin-at-pacbell.net } Date: Wednesday, October 12, 2011, 3:35 PM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } Does this work? } http://tinyurl.com/vacuum-meter } Thanks for the suggestion } Ken } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } } Sent: Wednesday, October 12, 2011 5:09 PM } To: kenconverse-at-qualityimages.biz } Subject: Re: [Microscopy] FW: Vacuum gauge reading } } Hey, Ken, } } Unfortunately, the list server truncated your URL. You } might want to try } something like tinyurl.com. } } --Justin. } } } On Oct 12, 2011, at 4:07 PM, kenconverse-at-qualityimages.biz } wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hi Jim, } } Take a look at } } } http://www2.snapfish.com/snapfish/thumbnailshare/AlbumID=3851367021/a=162307 } } } 140_162307140/otsc=SHR/otsi=SALBlink/COBRAND_NAME=snapfish/ } } These two meters are both on Varian Smart Gauges and I } think they } illustrate } } where some of the confusion is coming from. One } indicates where 1x10E-* } is } } located while the other indicates where the 10E-* } range (or decade) is } } located. Both meters cover the identical range and } can only be read } } correctly one way. } } } } Ken } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } -----Original Message----- } } X-from: James Ehrman [mailto:jehrman-at-mta.ca] } } Sent: Wednesday, October 12, 2011 3:19 PM } } To: kenconverse-at-qualityimages.biz } } Subject: Re: [Microscopy] RE: FW: Vacuum gauge } reading } } } } Hi Ken, } } } } As I wrote to Roger, I was explicitly trying *not* to } bring gauge } } calibration into this issue. My uncertainty here is } just with what } } freakin' value the gauge is telling me I have. One } view points to } } 2x10-6, the other to 2x10-5. I'm used to a measure of } uncertainty most } } things - order of magnitude uncertainty, no matter the } circumstances, is } } not really something I like to deal with. I think } Denton dropped the } } ball on labeling this gauge, no matter what the value } is supposed to be. } } } } Jim } } } } } } On 12/10/2011 4:12 PM, kenconverse-at-qualityimages.biz } wrote: } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Roger just said everything I was going to say. } If Denton did this } } } correctly, the gauge is reading 2x10E-6 (slightly } higher pressure than } } } 1x10E-6 to its left). If they screwed up the } calibration, shame on them. } } } Being a cold cathode gauge it may be in need of } both cleaning and } } } calibrating. } } } } } } In my experience if the 1x10E-5 was to indicate } the range (as on some } } Varian } } } gauges), then it would have been placed roughly } over the 4 in the range } } that } } } was intended. If it is placed on the end of a } range, it indicates } 1x10E-? } } } } } } My $.02 } } } } } } Ken Converse } } } owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } -----Original Message----- } } } X-from: raristau-at-ims.uconn.edu } [mailto:raristau-at-ims.uconn.edu] } } } Sent: Wednesday, October 12, 2011 2:32 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: [Microscopy] FW: Vacuum gauge reading } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } OK here comes Roger v1.3... } } } } } } I admit to being a little blindsided by Jim's } original posting, but I } hope } } } no harm was done by my erring on the side of } "didaction for dummies". } } Excuse } } } me if I did. My excuse: I am accustomed to } educating graduate students. } } } } } } Jim, if your sense is that the time of pumping was } insufficient to reach } } } 10e-6 range, therefore the meter MUST be } displaying 2 x 10e-5, perhaps } } there } } } is an issue of inaccurate, uncalibrated, or } otherwise deficient gauges. } } (Or } } } a really fast pump.) Most thermocouple and } ionizing gauges will become } } } inaccurate through buildup of surface deposits on } the gauge. Also they } are } } } calibrated for specific gases; a different gas } composition will result in } } an } } } inaccurate gauge. } } } } } } All my money is still on 2 x 10e-6. HOWEVER, one } caveat: If we get word } } from } } } the maker of the meter and they meant 10e-6 = 10 x } 10e-6, then I withdraw } } my } } } wager. But I would still insist that is an } improper way to label the } } meter. } } } } } } Still cheerful } } } Roger } } } } } } } } } ------ Forwarded Message } } } X-from: Roger Ristau {raristau-at-ims.uconn.edu} } } } Date: Wed, 12 Oct 2011 13:48:48 -0400 } } } To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } } } Conversation: [Microscopy] Vacuum gauge reading } } } Subject: FW: [Microscopy] Vacuum gauge reading } } } } } } A follow-up on my previous message: (I can't get } enough "out-of-office" } } } replies!) } } } } } } A tic marked 10e-6 means 1 x 10e-6. } } } It makes no sense for it to represent 10 x 10e-6; } that is an "improper } } } fraction". } } } } } } Cheers all } } } Roger } } } } } } ------ Forwarded Message } } } X-from: {raristau-at-ims.uconn.edu} } } } Reply-To: {raristau-at-ims.uconn.edu} } } } Date: Wed, 12 Oct 2011 11:53:12 -0500 } } } To: {raristau-at-ims.uconn.edu} } } } Subject: [Microscopy] Vacuum gauge reading } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } This has been a fun thread to follow.... } } } } } } With all humility and respect, it is plain that } Jim is not the only one } } } confused by these gauges. I have stared at a meter } for many minutes many } } } times as I tried to sort this out in my own head. } } } } } } Vacuum gauges, whether measuring current, voltage, } resistance, etc. are } } just } } } like most other meters: The lower, or smaller, or } least magnitude values } } are } } } on the left, the larger on the right. It is just } that the pressure gauge } } } starts with the needle pointing to the larger } value on the right (closer } } to } } } atmospheric pressure), and moves to the smaller } value end of the meter on } } } the left as the pumps improve the vacuum. We are } more accustomed to most } } } other sorts of gauges in which the needle starts } by pointing to the } } smaller } } } magnitude on the left and moves left to right as } the gadget it is } attached } } } to does its stuff. } } } } } } Therefore, the vacuum meter reading, as the needle } moves to the left of } } the } } } tic that is marked 10e-5 (= 1 x 10e-5), has } reached the 10e-6 range. Note } } } that the scale shows 8 (x 10e-6) to the right of 6 } (x 10e-6), that is, } the } } } larger magnitude to the right of the smaller } magnitude. The meter in } Jim's } } } image is reading just a hair higher than 2 x } 10e-6. } } } } } } Of course, not content to leave well enough alone, } we create most of our } } own } } } confusion when we refer to "higher vacuum" as the } state when the } magnitude } } } of pressure is "lower", and "lower vacuum" when } the pressure reading is } of } } } "higher" magnitude. But that is another thread... } } } } } } With smiles } } } Roger } } } } } } Roger A. Ristau, PhD } } } Electron Microscopy Specialist } } } Institute of Materials Science } } } 97 North Eagleville Road } } } University of Connecticut } } } Storrs, CT 06269 } } } vox: 860-486-5453 } } } fax: 860-486-4745 } } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 9, 21 -- From raristau-at-ims.uconn.edu } Wed Oct 12 11:50:51 2011 } } } 9, 21 -- Received: from mta2.uits.uconn.edu } (mta2.uits.uconn.edu } } } [137.99.25.235]) } } } 9, 21 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } } p9CGopcD011522 } } } 9, 21 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 11:50:51 -0500 } } } 9, 21 -- Received: from [137.99.20.184] } (d20h184.public.uconn.edu } } } [137.99.20.184]) } } } 9, 21 -- by mta2.uits.uconn.edu (Postfix) with } ESMTP id 1F7FC2CE43 } } } 9, 21 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 12:50:51 -0400 } } } (EDT) } } } 9, 21 -- User-Agent: } Microsoft-Entourage/11.4.0.080122 } } } 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 } } } 9, 21 -- Subject: Vacuum gauge reading } } } 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } } } 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } } } 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} } } } 9, 21 -- Thread-Topic: Vacuum gauge reading } } } 9, 21 -- Thread-Index: } AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== } } } 9, 21 -- Mime-version: 1.0 } } } 9, 21 -- Content-type: text/plain; } } } 9, 21 -- charset="US-ASCII" } } } 9, 21 -- Content-transfer-encoding: 7bit } } } 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at } mta2 } } } 9, 21 -- X-Virus-Status: Clean } } } ==============================End of - } } Headers============================== } } } } } } ------ End of Forwarded Message } } } } } } ------ End of Forwarded Message } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 28, 22 -- From raristau-at-ims.uconn.edu } Wed Oct 12 13:29:03 2011 } } } 28, 22 -- Received: from mta2.uits.uconn.edu } (mta2.uits.uconn.edu } } } [137.99.25.235]) } } } 28, 22 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } } p9CIT3Qf004544 } } } 28, 22 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 13:29:03 } } } -0500 } } } 28, 22 -- Received: from [137.99.20.184] } (d20h184.public.uconn.edu } } } [137.99.20.184]) } } } 28, 22 -- by mta2.uits.uconn.edu (Postfix) } with ESMTP id DFD4C2CFCA } } } 28, 22 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 14:29:02 } } } -0400 (EDT) } } } 28, 22 -- User-Agent: } Microsoft-Entourage/11.4.0.080122 } } } 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 } } } 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge } reading } } } 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } } } 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } } } 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} } } } 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge } reading } } } 28, 22 -- Thread-Index: } AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK } } } 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} } } } 28, 22 -- Mime-version: 1.0 } } } 28, 22 -- Content-type: text/plain; } } } 28, 22 -- charset="US-ASCII" } } } 28, 22 -- Content-transfer-encoding: 7bit } } } 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at } mta2 } } } 28, 22 -- X-Virus-Status: Clean } } } ==============================End of - } } Headers============================== } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 40, 28 -- From kenconverse-at-qualityimages.biz } Wed Oct 12 14:12:27 2011 } } } 40, 28 -- Received: from } cdptpa-omtalb.mail.rr.com } } (cdptpa-omtalb.mail.rr.com [75.180.132.121]) } } } 40, 28 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } p9CJCRaS002197 } } } 40, 28 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 14:12:27 } } -0500 } } } 40, 28 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW } c=1 sm=0 } } a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=a28-TuC6_4MA:10 } a=kj9zAlcOel0A:10 } } a=Zx37jsudAAAA:8 a=MKxSoT7or7n8rsCZ3E0A:9 } a=MjCJU8d-ybvQtNNfEm4A:7 } } a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 } a=L7AOxdDgvMIA:10 } } a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 } a=ZC8Yv9kwYYWqhca4:21 } } a=CTP1pKWpgRALcfUy:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } } } 40, 28 -- X-Cloudmark-Score: 0 } } } 40, 28 -- X-Originating-IP: 72.224.149.57 } } } 40, 28 -- Received: from [72.224.149.57] } ([72.224.149.57:2423] helo=Ken) } } } 40, 28 -- by cdptpa-oedge01.mail.rr.com } } (envelope-from {kenconverse-at-qualityimages.biz} ) } } } 40, 28 -- (ecelerity 2.2.3.46 r()) with } ESMTP } } } 40, 28 -- id C3/0A-06600-A96E59E4; Wed, 12 } Oct 2011 19:12:27 +0000 } } } 40, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } } 40, 28 -- To: {raristau-at-ims.uconn.edu} , } "MSA } } Listserver" {microscopy-at-microscopy.com} } } } 40, 28 -- Subject: RE: [Microscopy] FW: Vacuum } gauge reading } } } 40, 28 -- Date: Wed, 12 Oct 2011 15:12:20 -0400 } } } 40, 28 -- } Message-ID: {656CA10E51FE480792DFFCCF028FA6B3-at-Ken} } } } 40, 28 -- MIME-Version: 1.0 } } } 40, 28 -- Content-Type: text/plain; } } } 40, 28 -- charset="us-ascii" } } } 40, 28 -- X-Priority: 3 (Normal) } } } 40, 28 -- X-MSMail-Priority: Normal } } } 40, 28 -- X-Mailer: Microsoft Outlook, Build } 10.0.6863 } } } 40, 28 -- In-Reply-To: {201110121831.p9CIVpWw015451-at-ns.microscopy.com} } } } 40, 28 -- Importance: Normal } } } 40, 28 -- Thread-Index: } AcyJDTQvEHHpw1XfTzyWCbC6rI/RigABLkiw } } } 40, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.6109 } } } 40, 28 -- Content-Transfer-Encoding: 8bit } } } 40, 28 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by } } ns.microscopy.com id p9CJCRaS002197 } } } ==============================End of - } } Headers============================== } } } } } } -- } } } } James M. Ehrman } } Digital Microscopy Facility } } Mount Allison University } } 63B York St. } } Sackville, NB E4L 1G7 } } CANADA } } } } phone: 506-364-2519 } } fax: 506-364-2505 } } email: jehrman-at-mta.ca } } www: http://www.mta.ca/dmf } } } } "It's 5:50 a.m., Do you know where your stack pointer } is?" } } } } } } } } ==============================Original } Headers============================== } } 16, 29 -- From kenconverse-at-qualityimages.biz } Wed Oct 12 16:03:14 2011 } } 16, 29 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.122]) } } 16, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } p9CL3DnK010004 } } 16, 29 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 16:03:14 } -0500 } } 16, 29 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW c=1 } sm=0 } a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=zz6Rsj5XBPYA:10 } a=kj9zAlcOel0A:10 } a=1B0A9FJGAAAA:8 a=Zx37jsudAAAA:8 a=ayC55rCoAAAA:8 } a=ve1Z-OOgAAAA:8 } a=a1-IaBdjifOAZbIyw5IA:9 a=NoBNbbYosyDV6X2OgBwA:7 } a=CjuIK1q_8ugA:10 } a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 } a=RCW6_2rzkQAA:10 } a=2t4PYf3lEsYA:10 a=Nt0cOZSpmlUA:10 a=RyjLG6jaqdoA:10 } a=3_2eAsHTqJoA:10 } a=G91thX30KR8A:10 a=IegCt0Yb5t1AzbeG:21 } a=3EmC-Up7ZVry3oGO:21 } a=nqrs8wXCc9jj42pMp0m8Gw==:117 } } 16, 29 -- X-Cloudmark-Score: 0 } } 16, 29 -- X-Originating-IP: 72.224.149.57 } } 16, 29 -- Received: from [72.224.149.57] } ([72.224.149.57:3043] helo=Ken) } } 16, 29 -- by cdptpa-oedge01.mail.rr.com } (envelope-from } {kenconverse-at-qualityimages.biz} ) } } 16, 29 -- (ecelerity 2.2.3.46 r()) with ESMTP } } 16, 29 -- id 4E/72-06600-190069E4; Wed, 12 Oct } 2011 21:03:13 +0000 } } 16, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } 16, 29 -- To: "'James Ehrman'" {jehrman-at-mta.ca} , } } 16, 29 -- "MSA Listserver" {microscopy-at-microscopy.com} } } 16, 29 -- Subject: RE: [Microscopy] RE: FW: Vacuum } gauge reading } } 16, 29 -- Date: Wed, 12 Oct 2011 17:03:12 -0400 } } 16, 29 -- Message-ID: } {3735E2AB523A41E5A0147AAEE705232F-at-Ken} } } 16, 29 -- MIME-Version: 1.0 } } 16, 29 -- Content-Type: text/plain; } } 16, 29 -- charset="us-ascii" } } 16, 29 -- X-Priority: 3 (Normal) } } 16, 29 -- X-MSMail-Priority: Normal } } 16, 29 -- X-Mailer: Microsoft Outlook, Build } 10.0.6863 } } 16, 29 -- In-Reply-To: {4E95E815.800-at-mta.ca} } } 16, 29 -- Importance: Normal } } 16, 29 -- Thread-Index: } AcyJE8M/nS/mYUmiRXCumsSZGuJMrQADZ9bg } } 16, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.6109 } } 16, 29 -- Content-Transfer-Encoding: 8bit } } 16, 29 -- X-MIME-Autoconverted: from quoted-printable } to 8bit by } ns.microscopy.com id p9CL3DnK010004 } } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 11, 29 -- From kenconverse-at-qualityimages.biz } Wed Oct 12 17:31:42 2011 } 11, 29 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.121]) } 11, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id p9CMVg2x006934 } 11, 29 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 17:31:42 -0500 } 11, 29 -- X-Authority-Analysis: v=1.1 } cv=fBQ/umHr/NntWb97nPvjJqL5/J9L8K0X95NnniynOrI= c=1 sm=0 } a=uZF3FclSEUEA:10 a=kj9zAlcOel0A:10 } a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Wpj-ge2cAAAA:20 } a=pGLkceISAAAA:8 a=6D-Hy7tQAAAA:8 a=Zx37jsudAAAA:8 } a=1B0A9FJGAAAA:8 a=ayC55rCoAAAA:8 a=ve1Z-OOgAAAA:8 } a=1-aYQIqg--FAiBfwDjMA:9 a=Yj87RMty3e9NS65eD_sA:7 } a=CjuIK1q_8ugA:10 a=KXuLW65_DLEA:10 a=5JUlSvKOtR0A:10 } a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 a=RCW6_2rzkQAA:10 } a=2t4PYf3lEsYA:10 a=Nt0cOZSpmlUA:10 a=w5UaioRPqVMA:10 } a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=r8gzBB7U4WkA:10 } a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=WHgjr5aDOe_mPezi:21 } a=Q1TVv6reTxMO1jfe:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 11, 29 -- X-Cloudmark-Score: 0 } 11, 29 -- X-Originating-IP: 72.224.149.57 } 11, 29 -- Received: from [72.224.149.57] } ([72.224.149.57:3442] helo=Ken) } 11, 29 -- by cdptpa-oedge04.mail.rr.com } (envelope-from {kenconverse-at-qualityimages.biz} ) } 11, 29 -- (ecelerity 2.2.3.46 r()) with ESMTP } 11, 29 -- id E8/C6-05030-D45169E4; Wed, 12 Oct 2011 } 22:31:42 +0000 } 11, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 11, 29 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} , } 11, 29 -- "MSA Listserver" {microscopy-at-microscopy.com} } 11, 29 -- Subject: RE: [Microscopy] FW: Vacuum gauge } reading } 11, 29 -- Date: Wed, 12 Oct 2011 18:31:41 -0400 } 11, 29 -- Message-ID: } {03853FB1F1724A7081202F43ED68648F-at-Ken} } 11, 29 -- MIME-Version: 1.0 } 11, 29 -- Content-Type: text/plain; } 11, 29 -- charset="us-ascii" } 11, 29 -- X-Priority: 3 (Normal) } 11, 29 -- X-MSMail-Priority: Normal } 11, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 11, 29 -- In-Reply-To: {41CD7BBA-2F08-47B3-A76C-499A0C41B1AC-at-gmail.com} } 11, 29 -- Importance: Normal } 11, 29 -- Thread-Index: } AcyJIxvKndKApH+GTwmN5TIx8n7vrwAC3pdQ } 11, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.6109 } 11, 29 -- Content-Transfer-Encoding: 8bit } 11, 29 -- X-MIME-Autoconverted: from quoted-printable to } 8bit by ns.microscopy.com id p9CMVg2x006934 } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 6, 46 -- From kleopullin-at-pacbell.net Wed Oct 12 18:24:24 2011 6, 46 -- Received: from nm20-vm0.access.bullet.mail.sp2.yahoo.com (nm20-vm0.access.bullet.mail.sp2.yahoo.com [98.139.44.174]) 6, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9CNON8Z023990 6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 18:24:24 -0500 6, 46 -- Received: from [98.139.44.103] by nm20.access.bullet.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 23:24:23 -0000 6, 46 -- Received: from [98.139.44.78] by tm8.access.bullet.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 23:24:23 -0000 6, 46 -- Received: from [127.0.0.1] by omp1015.access.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 23:24:23 -0000 6, 46 -- X-Yahoo-Newman-Property: ymail-3 6, 46 -- X-Yahoo-Newman-Id: 422583.66014.bm-at-omp1015.access.mail.sp2.yahoo.com 6, 46 -- Received: (qmail 21555 invoked by uid 60001); 12 Oct 2011 23:24:23 -0000 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=pacbell.net; s=s1024; t=1318461863; bh=kymY3S3YFV/nttUzRcjB+OPr1WQmWmtmEJSFMQnmpMY=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=b+mz1uCr2IOfraOCntjVd9alTzvei2KlJKHfp6BLf0PentZUBQIT5ZQqqo3shTGZcAS/b8h0Ct+vkHy7fNrbf0wcmgtUUR5B5z45kFcdBYUS+cVIWkKfn8e8I5bv2XVOhKol5WdwLhRRgJcJs3RiUjcXqfvc5s9lHv7Rhl6wqCU= 6, 46 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 46 -- s=s1024; d=pacbell.net; 6, 46 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 46 -- b=UdHTvaSCD6jns0SyMgBKekF9VzoUtU5ifD2nN42ARjvEyzH0CnjZUZmrl5zKcb78lBeLiraRciS31OSWGx3miMZkC7h1KcoqQQhoRoyjzqSTiVs4ZBy56hvswtVcypVauekMz2+8OEKNmdoacn65ckPRj6B66IoyQaTjFcDNuDU=; 6, 46 -- X-YMail-OSG: EmiaE0EVM1mCdmhqscNHycD2iC5BRDUZazAA_oOinwnHl4. 6, 46 -- aIcIp4pDkAJ1.FGbzHOXPHt_uuM4L6_mrfKKpCO7ySqPnuLABCgTz8U2w2u8 6, 46 -- oVYzQkeNtd9fFs8eaZBeARin24EnMipugc1GGl7bgn4MR.7nLHd8eJhDvQTj 6, 46 -- mTRpZek6Vz7xekecVl5LLIMCqXYLXWChVB_TxvLgc8H_vub8BRhC1nq0tGso 6, 46 -- ZbYq06F4azDNt3908d_tbbqWenHfyP0UOb5iTd9SGxxaPH.eqEtRr.WQ8KWE 6, 46 -- bMpO3afuWSLu_Sef7FaJ__znd6R85xN8iSpB0a409cjmB211.Ioaw.KpLk8W 6, 46 -- j0JZiCDpnd60TAtdy_Qi44uYeYLFYubpzdQ4ur9wmUlqB617Cp6dWePbTXv5 6, 46 -- HU_mBa89NasXjT4hUxknbTbUYW2i9JJH0qWo4ujCtRtAOKTfYdlAeBW7hfIw 6, 46 -- nZtiNYA7._5ra1MMGM3O_RJ9m6pSYCDDiBOQap.xyIxYlvy.Wr1I8_76m4wm 6, 46 -- YXyqwxeOvcXn3WEQ4OJGks7wpScJiOOXVUhDK87IszhO33gvQu2faYEa6hQ7 6, 46 -- YhwSn3MNkhgKGZy0LCASxldTXQ2ZCnnIEYxJHyVnfCIhvB5UArALm1q.idv1 6, 46 -- 3suc5.mY9meP5axQWPhWaQjzYDZHf16mle2sE6Ta9ai8DDjoKL6Rgt8FFebm 6, 46 -- nLJkgtam5cSy6VgrmpKSFtA0Nbvnc6u67Iz6G4ak8LLtOuwfGV7VZ.T8SdtS 6, 46 -- aY6hn1l_qSGwEOICaipna4rXD3JqXToSh1TMy5C_F6kphZpv3TpjsztyPPDl 6, 46 -- njc977e9Vrhm24nD7i3QIcnv6qKu2yA2YiB9Wkw07WSYtVNIz3yAEHP2Yq51 6, 46 -- CTa8_JVgWYYR9AxA61uyXGdBrN1ds_VovPNlzDFuSoL7KmA6XRH1gmBznQbZ 6, 46 -- auLSxDmreZ0wvwUuZS31e0cG7263ZMK6gIsDSvZObIXT9kvhZ.Nbu8EsBN55 6, 46 -- QGfixuxTuIQtXjmtUPRzmGc1lB.KjlzdMzMjAGxe1RQIziYS2ght8Azy3G_J 6, 46 -- r5y7CNlOc77CjThUmnvaq6kTkpHZGU1WpdtOf 6, 46 -- Received: from [72.208.4.18] by web83401.mail.sp1.yahoo.com via HTTP; Wed, 12 Oct 2011 16:24:23 PDT 6, 46 -- X-Mailer: YahooMailClassic/14.0.7 YahooMailWebService/0.8.114.317681 6, 46 -- Message-ID: {1318461863.17818.YahooMailClassic-at-web83401.mail.sp1.yahoo.com} 6, 46 -- Date: Wed, 12 Oct 2011 16:24:23 -0700 (PDT) 6, 46 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 6, 46 -- Subject: Re: [Microscopy] RE: FW: Vacuum gauge reading 6, 46 -- To: Microscopy-at-microscopy.com 6, 46 -- In-Reply-To: {1318461721.20306.YahooMailClassic-at-web83401.mail.sp1.yahoo.com} 6, 46 -- MIME-Version: 1.0 6, 46 -- Content-Type: text/plain; charset=iso-8859-1 6, 46 -- Content-Transfer-Encoding: 8bit 6, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CNON8Z023990 ==============================End of - Headers==============================
Thanks to the support of the French Society of Microscopy and the Editions de Physique (EDP), all the papers in this issue are available in OPEN ACCESS.
We take this opportunity to thank our authors who took care to write their contribution for a broad audience and to invite you to submit your own manuscript soon.
Philippe A. Buffat and Mathieu Kociak, Guest editors Virginie Serin, President French Soc. Microscopies
Prof. Em. Philippe A. Buffat Ecole Polytechnique Federale de Lausanne AGH-UST University of Science and Technology Krakow philippe.buffat-at-epfl.ch, http://cime.epfl.ch
Associated Editor EPJAP, the European Physical Journal: Applied Physics*** (http://www.edpsciences.org/epjap/)
==============================Original Headers============================== 15, 19 -- From philippe.buffat-at-epfl.ch Wed Oct 12 18:26:37 2011 15, 19 -- Received: from smtp4.epfl.ch (smtp4.epfl.ch [128.178.224.218]) 15, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9CNQaQg027261 15, 19 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 18:26:36 -0500 15, 19 -- Received: (qmail 20835 invoked by uid 107); 12 Oct 2011 21:39:52 -0000 15, 19 -- X-Virus-Scanned: ClamAV 15, 19 -- Received: from vpn-b-195-166.epfl.ch (HELO discarded) (128.178.195.166) (authenticated) 15, 19 -- by smtp4.epfl.ch (AngelmatoPhylax SMTP proxy) with ESMTPA; Wed, 12 Oct 2011 23:39:53 +0200 15, 19 -- Message-ID: {4E960921.8020000-at-epfl.ch} 15, 19 -- Date: Wed, 12 Oct 2011 23:39:45 +0200 15, 19 -- From: Philippe Buffat {philippe.buffat-at-epfl.ch} 15, 19 -- Reply-To: philippe.buffat-at-epfl.ch 15, 19 -- Organization: EPFL 15, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:5.0) Gecko/20110624 Thunderbird/5.0 15, 19 -- MIME-Version: 1.0 15, 19 -- To: Microscopy-at-microscopy.com 15, 19 -- Subject: STEM/EELS/Time resolved/special issue C. Colliex 15, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 15, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Also, the gauge has shorter lines pointing to the coefficients and the right number of longer lines pointing to each of the exponents. It does seem that there is only one way to associate the exponents and their coefficients, with the longer tick marks pointing to the exponents, and accounting for their being a single set of coefficients associated with each exponent and each exponent associated with a set of coefficients.
I initially found it tricky learning to read vacuum gauges like this in EM53 (EM maintenance and repair) at Delta. I thoroughly enjoyed reading this thread from a "yeah! the professionals struggle, too," perspective
Thanks, Kleo
--- On Wed, 10/12/11, Kleo Pullin {kleopullin-at-pacbell.net} wrote:
} From: Kleo Pullin {kleopullin-at-pacbell.net} } Subject: Re: [Microscopy] RE: FW: Vacuum gauge reading } To: KLeoPullin-at-pacbell.net, kenconverse-at-qualityimages.biz } Date: Wednesday, October 12, 2011, 4:22 PM } If this reads 10-5, the first set of } coefficients on the right side of the gauge has no exponent } associated with it, and the last exponent (10-9) has no } coefficients. } } If it reads 10-6, then each exponent has a set of } coefficients and vice versa. } } Kleo } } --- On Wed, 10/12/11, kenconverse-at-qualityimages.biz } {kenconverse-at-qualityimages.biz} } wrote: } } From: kenconverse-at-qualityimages.biz } {kenconverse-at-qualityimages.biz} } Subject: [Microscopy] RE: FW: Vacuum gauge reading } To: KLeoPullin-at-pacbell.net } Date: Wednesday, October 12, 2011, 3:35 PM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy } Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Justin, } Does this work? } http://tinyurl.com/vacuum-meter } Thanks for the suggestion } Ken } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: Justin Kraft [mailto:kraftpiano-at-gmail.com] } } Sent: Wednesday, October 12, 2011 5:09 PM } To: kenconverse-at-qualityimages.biz } Subject: Re: [Microscopy] FW: Vacuum gauge reading } } Hey, Ken, } } Unfortunately, the list server truncated your URL. You } might want to try } something like tinyurl.com. } } --Justin. } } } On Oct 12, 2011, at 4:07 PM, kenconverse-at-qualityimages.biz } wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of America } } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } Hi Jim, } } Take a look at } } } http://www2.snapfish.com/snapfish/thumbnailshare/AlbumID=3851367021/a=162307 } } } 140_162307140/otsc=SHR/otsi=SALBlink/COBRAND_NAME=snapfish/ } } These two meters are both on Varian Smart Gauges and I } think they } illustrate } } where some of the confusion is coming from. One } indicates where 1x10E-* } is } } located while the other indicates where the 10E-* } range (or decade) is } } located. Both meters cover the identical range and } can only be read } } correctly one way. } } } } Ken } } } } QUALITY IMAGES } } Servicing Scanning Electron Microscopes } } Since 1981 } } 474 So. Bridgton Rd. } } Bridgton, ME 04009 } } 207-647-4348 } } kenconverse-at-qualityimages.biz } } qualityimages.biz } } } } } } -----Original Message----- } } X-from: James Ehrman [mailto:jehrman-at-mta.ca] } } Sent: Wednesday, October 12, 2011 3:19 PM } } To: kenconverse-at-qualityimages.biz } } Subject: Re: [Microscopy] RE: FW: Vacuum gauge } reading } } } } Hi Ken, } } } } As I wrote to Roger, I was explicitly trying *not* to } bring gauge } } calibration into this issue. My uncertainty here is } just with what } } freakin' value the gauge is telling me I have. One } view points to } } 2x10-6, the other to 2x10-5. I'm used to a measure of } uncertainty most } } things - order of magnitude uncertainty, no matter the } circumstances, is } } not really something I like to deal with. I think } Denton dropped the } } ball on labeling this gauge, no matter what the value } is supposed to be. } } } } Jim } } } } } } On 12/10/2011 4:12 PM, kenconverse-at-qualityimages.biz } wrote: } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } Roger just said everything I was going to say. } If Denton did this } } } correctly, the gauge is reading 2x10E-6 (slightly } higher pressure than } } } 1x10E-6 to its left). If they screwed up the } calibration, shame on them. } } } Being a cold cathode gauge it may be in need of } both cleaning and } } } calibrating. } } } } } } In my experience if the 1x10E-5 was to indicate } the range (as on some } } Varian } } } gauges), then it would have been placed roughly } over the 4 in the range } } that } } } was intended. If it is placed on the end of a } range, it indicates } 1x10E-? } } } } } } My $.02 } } } } } } Ken Converse } } } owner } } } } } } QUALITY IMAGES } } } Servicing Scanning Electron Microscopes } } } Since 1981 } } } 474 So. Bridgton Rd. } } } Bridgton, ME 04009 } } } 207-647-4348 } } } kenconverse-at-qualityimages.biz } } } qualityimages.biz } } } } } } } } } -----Original Message----- } } } X-from: raristau-at-ims.uconn.edu } [mailto:raristau-at-ims.uconn.edu] } } } Sent: Wednesday, October 12, 2011 2:32 PM } } } To: kenconverse-at-qualityimages.biz } } } Subject: [Microscopy] FW: Vacuum gauge reading } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } OK here comes Roger v1.3... } } } } } } I admit to being a little blindsided by Jim's } original posting, but I } hope } } } no harm was done by my erring on the side of } "didaction for dummies". } } Excuse } } } me if I did. My excuse: I am accustomed to } educating graduate students. } } } } } } Jim, if your sense is that the time of pumping was } insufficient to reach } } } 10e-6 range, therefore the meter MUST be } displaying 2 x 10e-5, perhaps } } there } } } is an issue of inaccurate, uncalibrated, or } otherwise deficient gauges. } } (Or } } } a really fast pump.) Most thermocouple and } ionizing gauges will become } } } inaccurate through buildup of surface deposits on } the gauge. Also they } are } } } calibrated for specific gases; a different gas } composition will result in } } an } } } inaccurate gauge. } } } } } } All my money is still on 2 x 10e-6. HOWEVER, one } caveat: If we get word } } from } } } the maker of the meter and they meant 10e-6 = 10 x } 10e-6, then I withdraw } } my } } } wager. But I would still insist that is an } improper way to label the } } meter. } } } } } } Still cheerful } } } Roger } } } } } } } } } ------ Forwarded Message } } } X-from: Roger Ristau {raristau-at-ims.uconn.edu} } } } Date: Wed, 12 Oct 2011 13:48:48 -0400 } } } To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } } } Conversation: [Microscopy] Vacuum gauge reading } } } Subject: FW: [Microscopy] Vacuum gauge reading } } } } } } A follow-up on my previous message: (I can't get } enough "out-of-office" } } } replies!) } } } } } } A tic marked 10e-6 means 1 x 10e-6. } } } It makes no sense for it to represent 10 x 10e-6; } that is an "improper } } } fraction". } } } } } } Cheers all } } } Roger } } } } } } ------ Forwarded Message } } } X-from: {raristau-at-ims.uconn.edu} } } } Reply-To: {raristau-at-ims.uconn.edu} } } } Date: Wed, 12 Oct 2011 11:53:12 -0500 } } } To: {raristau-at-ims.uconn.edu} } } } Subject: [Microscopy] Vacuum gauge reading } } } } } } } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The } Microscopy Society of } America } } } To Subscribe/Unsubscribe -- } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } } ---------------------------------------------------------------------------- } } } } } } This has been a fun thread to follow.... } } } } } } With all humility and respect, it is plain that } Jim is not the only one } } } confused by these gauges. I have stared at a meter } for many minutes many } } } times as I tried to sort this out in my own head. } } } } } } Vacuum gauges, whether measuring current, voltage, } resistance, etc. are } } just } } } like most other meters: The lower, or smaller, or } least magnitude values } } are } } } on the left, the larger on the right. It is just } that the pressure gauge } } } starts with the needle pointing to the larger } value on the right (closer } } to } } } atmospheric pressure), and moves to the smaller } value end of the meter on } } } the left as the pumps improve the vacuum. We are } more accustomed to most } } } other sorts of gauges in which the needle starts } by pointing to the } } smaller } } } magnitude on the left and moves left to right as } the gadget it is } attached } } } to does its stuff. } } } } } } Therefore, the vacuum meter reading, as the needle } moves to the left of } } the } } } tic that is marked 10e-5 (= 1 x 10e-5), has } reached the 10e-6 range. Note } } } that the scale shows 8 (x 10e-6) to the right of 6 } (x 10e-6), that is, } the } } } larger magnitude to the right of the smaller } magnitude. The meter in } Jim's } } } image is reading just a hair higher than 2 x } 10e-6. } } } } } } Of course, not content to leave well enough alone, } we create most of our } } own } } } confusion when we refer to "higher vacuum" as the } state when the } magnitude } } } of pressure is "lower", and "lower vacuum" when } the pressure reading is } of } } } "higher" magnitude. But that is another thread... } } } } } } With smiles } } } Roger } } } } } } Roger A. Ristau, PhD } } } Electron Microscopy Specialist } } } Institute of Materials Science } } } 97 North Eagleville Road } } } University of Connecticut } } } Storrs, CT 06269 } } } vox: 860-486-5453 } } } fax: 860-486-4745 } } } } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 9, 21 -- From raristau-at-ims.uconn.edu } Wed Oct 12 11:50:51 2011 } } } 9, 21 -- Received: from mta2.uits.uconn.edu } (mta2.uits.uconn.edu } } } [137.99.25.235]) } } } 9, 21 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } } p9CGopcD011522 } } } 9, 21 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 11:50:51 -0500 } } } 9, 21 -- Received: from [137.99.20.184] } (d20h184.public.uconn.edu } } } [137.99.20.184]) } } } 9, 21 -- by mta2.uits.uconn.edu (Postfix) with } ESMTP id 1F7FC2CE43 } } } 9, 21 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 12:50:51 -0400 } } } (EDT) } } } 9, 21 -- User-Agent: } Microsoft-Entourage/11.4.0.080122 } } } 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 } } } 9, 21 -- Subject: Vacuum gauge reading } } } 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } } } 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } } } 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} } } } 9, 21 -- Thread-Topic: Vacuum gauge reading } } } 9, 21 -- Thread-Index: } AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== } } } 9, 21 -- Mime-version: 1.0 } } } 9, 21 -- Content-type: text/plain; } } } 9, 21 -- charset="US-ASCII" } } } 9, 21 -- Content-transfer-encoding: 7bit } } } 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at } mta2 } } } 9, 21 -- X-Virus-Status: Clean } } } ==============================End of - } } Headers============================== } } } } } } ------ End of Forwarded Message } } } } } } ------ End of Forwarded Message } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 28, 22 -- From raristau-at-ims.uconn.edu } Wed Oct 12 13:29:03 2011 } } } 28, 22 -- Received: from mta2.uits.uconn.edu } (mta2.uits.uconn.edu } } } [137.99.25.235]) } } } 28, 22 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } } p9CIT3Qf004544 } } } 28, 22 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 13:29:03 } } } -0500 } } } 28, 22 -- Received: from [137.99.20.184] } (d20h184.public.uconn.edu } } } [137.99.20.184]) } } } 28, 22 -- by mta2.uits.uconn.edu (Postfix) } with ESMTP id DFD4C2CFCA } } } 28, 22 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 14:29:02 } } } -0400 (EDT) } } } 28, 22 -- User-Agent: } Microsoft-Entourage/11.4.0.080122 } } } 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 } } } 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge } reading } } } 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } } } 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } } } 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} } } } 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge } reading } } } 28, 22 -- Thread-Index: } AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK } } } 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} } } } 28, 22 -- Mime-version: 1.0 } } } 28, 22 -- Content-type: text/plain; } } } 28, 22 -- charset="US-ASCII" } } } 28, 22 -- Content-transfer-encoding: 7bit } } } 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at } mta2 } } } 28, 22 -- X-Virus-Status: Clean } } } ==============================End of - } } Headers============================== } } } } } } } } } } } } ==============================Original } } Headers============================== } } } 40, 28 -- From kenconverse-at-qualityimages.biz } Wed Oct 12 14:12:27 2011 } } } 40, 28 -- Received: from } cdptpa-omtalb.mail.rr.com } } (cdptpa-omtalb.mail.rr.com [75.180.132.121]) } } } 40, 28 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } } p9CJCRaS002197 } } } 40, 28 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 14:12:27 } } -0500 } } } 40, 28 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW } c=1 sm=0 } } a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=a28-TuC6_4MA:10 } a=kj9zAlcOel0A:10 } } a=Zx37jsudAAAA:8 a=MKxSoT7or7n8rsCZ3E0A:9 } a=MjCJU8d-ybvQtNNfEm4A:7 } } a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 } a=L7AOxdDgvMIA:10 } } a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 } a=ZC8Yv9kwYYWqhca4:21 } } a=CTP1pKWpgRALcfUy:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } } } 40, 28 -- X-Cloudmark-Score: 0 } } } 40, 28 -- X-Originating-IP: 72.224.149.57 } } } 40, 28 -- Received: from [72.224.149.57] } ([72.224.149.57:2423] helo=Ken) } } } 40, 28 -- by cdptpa-oedge01.mail.rr.com } } (envelope-from {kenconverse-at-qualityimages.biz} ) } } } 40, 28 -- (ecelerity 2.2.3.46 r()) with } ESMTP } } } 40, 28 -- id C3/0A-06600-A96E59E4; Wed, 12 } Oct 2011 19:12:27 +0000 } } } 40, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } } 40, 28 -- To: {raristau-at-ims.uconn.edu} , } "MSA } } Listserver" {microscopy-at-microscopy.com} } } } 40, 28 -- Subject: RE: [Microscopy] FW: Vacuum } gauge reading } } } 40, 28 -- Date: Wed, 12 Oct 2011 15:12:20 -0400 } } } 40, 28 -- } Message-ID: {656CA10E51FE480792DFFCCF028FA6B3-at-Ken} } } } 40, 28 -- MIME-Version: 1.0 } } } 40, 28 -- Content-Type: text/plain; } } } 40, 28 -- charset="us-ascii" } } } 40, 28 -- X-Priority: 3 (Normal) } } } 40, 28 -- X-MSMail-Priority: Normal } } } 40, 28 -- X-Mailer: Microsoft Outlook, Build } 10.0.6863 } } } 40, 28 -- In-Reply-To: {201110121831.p9CIVpWw015451-at-ns.microscopy.com} } } } 40, 28 -- Importance: Normal } } } 40, 28 -- Thread-Index: } AcyJDTQvEHHpw1XfTzyWCbC6rI/RigABLkiw } } } 40, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.6109 } } } 40, 28 -- Content-Transfer-Encoding: 8bit } } } 40, 28 -- X-MIME-Autoconverted: from } quoted-printable to 8bit by } } ns.microscopy.com id p9CJCRaS002197 } } } ==============================End of - } } Headers============================== } } } } } } -- } } } } James M. Ehrman } } Digital Microscopy Facility } } Mount Allison University } } 63B York St. } } Sackville, NB E4L 1G7 } } CANADA } } } } phone: 506-364-2519 } } fax: 506-364-2505 } } email: jehrman-at-mta.ca } } www: http://www.mta.ca/dmf } } } } "It's 5:50 a.m., Do you know where your stack pointer } is?" } } } } } } } } ==============================Original } Headers============================== } } 16, 29 -- From kenconverse-at-qualityimages.biz } Wed Oct 12 16:03:14 2011 } } 16, 29 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.122]) } } 16, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } p9CL3DnK010004 } } 16, 29 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 16:03:14 } -0500 } } 16, 29 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW c=1 } sm=0 } a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=zz6Rsj5XBPYA:10 } a=kj9zAlcOel0A:10 } a=1B0A9FJGAAAA:8 a=Zx37jsudAAAA:8 a=ayC55rCoAAAA:8 } a=ve1Z-OOgAAAA:8 } a=a1-IaBdjifOAZbIyw5IA:9 a=NoBNbbYosyDV6X2OgBwA:7 } a=CjuIK1q_8ugA:10 } a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 } a=RCW6_2rzkQAA:10 } a=2t4PYf3lEsYA:10 a=Nt0cOZSpmlUA:10 a=RyjLG6jaqdoA:10 } a=3_2eAsHTqJoA:10 } a=G91thX30KR8A:10 a=IegCt0Yb5t1AzbeG:21 } a=3EmC-Up7ZVry3oGO:21 } a=nqrs8wXCc9jj42pMp0m8Gw==:117 } } 16, 29 -- X-Cloudmark-Score: 0 } } 16, 29 -- X-Originating-IP: 72.224.149.57 } } 16, 29 -- Received: from [72.224.149.57] } ([72.224.149.57:3043] helo=Ken) } } 16, 29 -- by cdptpa-oedge01.mail.rr.com } (envelope-from } {kenconverse-at-qualityimages.biz} ) } } 16, 29 -- (ecelerity 2.2.3.46 r()) with ESMTP } } 16, 29 -- id 4E/72-06600-190069E4; Wed, 12 Oct } 2011 21:03:13 +0000 } } 16, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } } 16, 29 -- To: "'James Ehrman'" {jehrman-at-mta.ca} , } } 16, 29 -- "MSA Listserver" {microscopy-at-microscopy.com} } } 16, 29 -- Subject: RE: [Microscopy] RE: FW: Vacuum } gauge reading } } 16, 29 -- Date: Wed, 12 Oct 2011 17:03:12 -0400 } } 16, 29 -- Message-ID: } {3735E2AB523A41E5A0147AAEE705232F-at-Ken} } } 16, 29 -- MIME-Version: 1.0 } } 16, 29 -- Content-Type: text/plain; } } 16, 29 -- charset="us-ascii" } } 16, 29 -- X-Priority: 3 (Normal) } } 16, 29 -- X-MSMail-Priority: Normal } } 16, 29 -- X-Mailer: Microsoft Outlook, Build } 10.0.6863 } } 16, 29 -- In-Reply-To: {4E95E815.800-at-mta.ca} } } 16, 29 -- Importance: Normal } } 16, 29 -- Thread-Index: } AcyJE8M/nS/mYUmiRXCumsSZGuJMrQADZ9bg } } 16, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.6109 } } 16, 29 -- Content-Transfer-Encoding: 8bit } } 16, 29 -- X-MIME-Autoconverted: from quoted-printable } to 8bit by } ns.microscopy.com id p9CL3DnK010004 } } ==============================End of - } Headers============================== } } } } ==============================Original } Headers============================== } 11, 29 -- From kenconverse-at-qualityimages.biz } Wed Oct 12 17:31:42 2011 } 11, 29 -- Received: from cdptpa-omtalb.mail.rr.com } (cdptpa-omtalb.mail.rr.com [75.180.132.121]) } 11, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id p9CMVg2x006934 } 11, 29 -- for {microscopy-at-microscopy.com} ; } Wed, 12 Oct 2011 17:31:42 -0500 } 11, 29 -- X-Authority-Analysis: v=1.1 } cv=fBQ/umHr/NntWb97nPvjJqL5/J9L8K0X95NnniynOrI= c=1 sm=0 } a=uZF3FclSEUEA:10 a=kj9zAlcOel0A:10 } a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=Wpj-ge2cAAAA:20 } a=pGLkceISAAAA:8 a=6D-Hy7tQAAAA:8 a=Zx37jsudAAAA:8 } a=1B0A9FJGAAAA:8 a=ayC55rCoAAAA:8 a=ve1Z-OOgAAAA:8 } a=1-aYQIqg--FAiBfwDjMA:9 a=Yj87RMty3e9NS65eD_sA:7 } a=CjuIK1q_8ugA:10 a=KXuLW65_DLEA:10 a=5JUlSvKOtR0A:10 } a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 a=RCW6_2rzkQAA:10 } a=2t4PYf3lEsYA:10 a=Nt0cOZSpmlUA:10 a=w5UaioRPqVMA:10 } a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=r8gzBB7U4WkA:10 } a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 a=WHgjr5aDOe_mPezi:21 } a=Q1TVv6reTxMO1jfe:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 11, 29 -- X-Cloudmark-Score: 0 } 11, 29 -- X-Originating-IP: 72.224.149.57 } 11, 29 -- Received: from [72.224.149.57] } ([72.224.149.57:3442] helo=Ken) } 11, 29 -- by cdptpa-oedge04.mail.rr.com } (envelope-from {kenconverse-at-qualityimages.biz} ) } 11, 29 -- (ecelerity 2.2.3.46 r()) with ESMTP } 11, 29 -- id E8/C6-05030-D45169E4; Wed, 12 Oct 2011 } 22:31:42 +0000 } 11, 29 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 11, 29 -- To: "'Justin Kraft'" {kraftpiano-at-gmail.com} , } 11, 29 -- "MSA Listserver" {microscopy-at-microscopy.com} } 11, 29 -- Subject: RE: [Microscopy] FW: Vacuum gauge } reading } 11, 29 -- Date: Wed, 12 Oct 2011 18:31:41 -0400 } 11, 29 -- Message-ID: } {03853FB1F1724A7081202F43ED68648F-at-Ken} } 11, 29 -- MIME-Version: 1.0 } 11, 29 -- Content-Type: text/plain; } 11, 29 -- charset="us-ascii" } 11, 29 -- X-Priority: 3 (Normal) } 11, 29 -- X-MSMail-Priority: Normal } 11, 29 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 11, 29 -- In-Reply-To: {41CD7BBA-2F08-47B3-A76C-499A0C41B1AC-at-gmail.com} } 11, 29 -- Importance: Normal } 11, 29 -- Thread-Index: } AcyJIxvKndKApH+GTwmN5TIx8n7vrwAC3pdQ } 11, 29 -- X-MimeOLE: Produced By Microsoft MimeOLE } V6.00.2900.6109 } 11, 29 -- Content-Transfer-Encoding: 8bit } 11, 29 -- X-MIME-Autoconverted: from quoted-printable to } 8bit by ns.microscopy.com id p9CMVg2x006934 } ==============================End of - } Headers============================== } }
==============================Original Headers============================== 6, 46 -- From kleopullin-at-pacbell.net Wed Oct 12 18:54:05 2011 6, 46 -- Received: from nm13.access.bullet.mail.sp2.yahoo.com (nm13.access.bullet.mail.sp2.yahoo.com [98.139.44.140]) 6, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9CNs4sO025084 6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 18:54:05 -0500 6, 46 -- Received: from [98.139.44.105] by nm13.access.bullet.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 23:54:04 -0000 6, 46 -- Received: from [98.139.44.78] by tm10.access.bullet.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 23:54:04 -0000 6, 46 -- Received: from [127.0.0.1] by omp1015.access.mail.sp2.yahoo.com with NNFMP; 12 Oct 2011 23:54:04 -0000 6, 46 -- X-Yahoo-Newman-Property: ymail-3 6, 46 -- X-Yahoo-Newman-Id: 502484.99448.bm-at-omp1015.access.mail.sp2.yahoo.com 6, 46 -- Received: (qmail 44274 invoked by uid 60001); 12 Oct 2011 23:54:04 -0000 6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=pacbell.net; s=s1024; t=1318463644; bh=I6WOAcCyiusEg7w66B7RsBrGGnu0ooucHvs1ex48ZBk=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=wDxy56oYMQlzT6v3PzT20qA6SNAWxOx5XBENyM3/99G1klI0bfbPbJbGfLR9jRCOqEUZ4/V1Xs8AHrQa78DQ6NuJRIAWaO7fJs0um/ZjE4XPqb4Zzwi5i7LIc0qjFL6QvTaHthTSBO93GEwQLma9hS43cN15KzORuRW7RFus/Ks= 6, 46 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 6, 46 -- s=s1024; d=pacbell.net; 6, 46 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 6, 46 -- b=u1QhghdfCPdOeU2lZlw74m0RYifm7B6COndFLb9aNcXbJvbbcLDlXO0yBCj+Knd8NbDjq6vVjb1oTTK1rmT7RZQk+tmpQWL9cMdk5KMr3PKTZyzm44xeAZGdArrR6bKjHL6UvPELAuE12Ea4VpuAc//YrELvGiEnzvWJbz/2DiQ=; 6, 46 -- X-YMail-OSG: DRlLcogVM1nu4Dc48UbiUZLLkYSiW01Tzoab1L9V2eJo9F2 6, 46 -- Kzt2kVPf2BvB_nsy0fLCaOxJuCa.sjZQ9uaN.BOC.hS2Kl1URtH_HIXqGQVa 6, 46 -- Carg2pNo2MJf6bmiNwXGqwm2d5vrYinv3zJZlKV9wsbvqsCADOB7Bgq7spv1 6, 46 -- NJ.DZNbJWDZM_w_skmdMXvLMEOqgQhf5F49wls3kwfG_m2eBa2MvzcpFmS0L 6, 46 -- bjzWpzo6wXXmJ.B0zypXw17LbYV9XJXK_MC3XZP1cjsngCmNIeiR5BH7gtP8 6, 46 -- 5dpQV3Un8li8RdT4VqiESfG4A8H4QYhcT2YMjVRWlkZRp6Unnfky6jQEbJzj 6, 46 -- C18AIypMSX56.Su9osA.AT7JDSvIDZrShm7SaR0XMBi1w9KTG0MYaPu4bIqb 6, 46 -- 4KH3Kfl0UqV6ytlvBHk6JmxLWf8IY33vCQcFeODIkQ41Z1nTWwnEyVZOgyYe 6, 46 -- tVh9.oXUgEA9EpWvHTjTm6SF.G8WNNo8AS9c8QiY1kFPG5WhekMAPPz9TtND 6, 46 -- Lco0a9in.kJwuHaukWRTj52MtBmcJigfDPKDGYXzPpVeL3jIQkglo.3jby6p 6, 46 -- hG.EmDpWbZm0kZ14HXRu_Qrm5k.IehvF.Jy7F4iDgR7dc2ZsIKn3UtvFWHiP 6, 46 -- 4i7ZIbibokPDyeJJwqCj4VkuOHGLCa_WgiSjB08JSzhsPmw4qA3FX71YT1CF 6, 46 -- 4kTuGB8gafShbuZCR7PRUQKL7lxQ1wi.P8SdxwTe_lIdhvH0q1oIF3um2kOT 6, 46 -- WW8UPRatKpkipMzt7VMui5h2Faw5puXxVk0t7K3a5H92w6tm3shqIsZ9u5ww 6, 46 -- x9a23dTL8rBzMEXm0rGZlrlySLpUSreDGRJQnCtpVWsZzPf4VFPHpJB6FBMz 6, 46 -- .CQKo0LmfI.6Bg8OqMQY4lLdXV8nNlCUnDvRIRXro8tdJl6Aon5mgsDiD_.Q 6, 46 -- kUuw20wwSVNjI4nCPdIgxelxEJfPYK4FMhw4o4bFSyiTrxBLCF3W2VPgrOfE 6, 46 -- Mgv5tWShXrOz0P8f05SpbbPGoX0kNcqlgS6TLcFFhP3Nosl.Nz6kfcwbUvG7 6, 46 -- oDfGz3V7sAog.pXtVJjQQ3T00dV0Ch2g38SZ9 6, 46 -- Received: from [72.208.4.18] by web83403.mail.sp1.yahoo.com via HTTP; Wed, 12 Oct 2011 16:54:03 PDT 6, 46 -- X-Mailer: YahooMailClassic/14.0.7 YahooMailWebService/0.8.114.317681 6, 46 -- Message-ID: {1318463643.41211.YahooMailClassic-at-web83403.mail.sp1.yahoo.com} 6, 46 -- Date: Wed, 12 Oct 2011 16:54:03 -0700 (PDT) 6, 46 -- From: Kleo Pullin {kleopullin-at-pacbell.net} 6, 46 -- Subject: Re: [Microscopy] RE: Vacuum gauge reading 6, 46 -- To: Microscopy-at-microscopy.com 6, 46 -- In-Reply-To: {1318461721.20306.YahooMailClassic-at-web83401.mail.sp1.yahoo.com} 6, 46 -- MIME-Version: 1.0 6, 46 -- Content-Type: text/plain; charset=iso-8859-1 6, 46 -- Content-Transfer-Encoding: 8bit 6, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CNs4sO025084 ==============================End of - Headers==============================
OK, I have sat patiently through all of this discussion and now I feel I have to comment. What on earth is the problem here? It's a gauge, just read it!
___
John Mansfield PhD Cphys MInstP 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
==============================Original Headers============================== 7, 20 -- From jfmjfm-at-umich.edu Wed Oct 12 19:15:10 2011 7, 20 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 7, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9D0F9VA009432 7, 20 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 19:15:10 -0500 7, 20 -- Received: FROM [10.0.1.31] (c-68-40-166-190.hsd1.mi.comcast.net [68.40.166.190]) 7, 20 -- By hellskitchen.mr.itd.umich.edu ID 4E962D8B.56F74.1100 ; 7, 20 -- Authuser jfmjfm; 7, 20 -- 12 Oct 2011 20:15:07 EDT 7, 20 -- Subject: Re: [Microscopy] Vacuum gauge reading 7, 20 -- Mime-Version: 1.0 (Apple Message framework v1244.3) 7, 20 -- Content-Type: text/plain; charset=iso-8859-1 7, 20 -- From: John Mansfield {jfmjfm-at-umich.edu} 7, 20 -- In-Reply-To: {201110130003.p9D03UwN006054-at-ns.microscopy.com} 7, 20 -- Date: Wed, 12 Oct 2011 20:15:05 -0400 7, 20 -- Message-Id: {0887A237-7D81-4FC1-879F-F986F2675F28-at-umich.edu} 7, 20 -- References: {201110130003.p9D03UwN006054-at-ns.microscopy.com} 7, 20 -- To: microscopy-at-microscopy.com 7, 20 -- X-Mailer: Apple Mail (2.1244.3) 7, 20 -- Content-Transfer-Encoding: 8bit 7, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9D0F9VA009432 ==============================End of - Headers==============================
More air to the right. Less air to the left. But wait! That was my right and my left. Not the instrument right and left. I am right handed. No idea how the instrument is. I assumed we both right handed which could be false.
First and most the scale graduated in Torr. Not in Torr x 10, not in milliTorr. The "Torr" is the largest thing on the face of the meter. (I love Denton Vacuum but it is not a unit of measure yet - here is an idea...)
Move needle to the right and pressure becomes 5x10-6, then 8x10-6, then 10x10-6 which equals 10-5. More air more pressure.
Move needle to the left and it becomes 1x10-6 which equals 10-6 or a half of the displayed reading. The 5x10-7 is further to the left and is half of 10-6. The 10-7 still further to the left and is 1/10 of 10-6 or 1/20 of 2x10-6. Less air less pressure.
Question though is purely educational because cold cathode gauges accurate to the order of magnitude. Sure I can calibrate such gauge for a bet. Reading will drift within months in a relatively stable environment such as TEM. But dust and carbon inside the evaporator will make reading dependent on temperature/vibration within days of practical use. The safe statement should be "low 10-6 Torr range". Which is a decent vacuum for most preparation methods.
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
On 10/12/2011 9:12 AM, jehrman-at-mta.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Listers, } } This is a bit embarrassing, but I've been bothered by this long enough. } Check out this picture of the vacuum gauge from my vacuum evaporator: } } http://www.mta.ca/dmf/download/jme/imgp0520a.jpg } } My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My } feeling for typical vacuum system performance tells me it's the former, } but the labeling on the gauge is somewhat ambiguous. Does the large } "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems } I've used didn't leave this open to interpretation. } } Thanks in advance to those who respond and help dispel my uneasiness... } } Jim } }
Here is an image of an old Balzers gauge: {http://www2.biomed.cas.cz/~benada/V_meter_Balzers.jpg} The Balzers gauge graphics shows it clearly (around 8x10-5 torrs, in this case).
Best regards from Prague. Oldrich
On Wednesday 12 of October 2011 15:13:23 jehrman-at-mta.ca wrote: } --------------------------------------------------------------------------- } - The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } -------------------------------------------------------------------------- } -- } } Hi Listers, } } This is a bit embarrassing, but I've been bothered by this long enough. } Check out this picture of the vacuum gauge from my vacuum evaporator: } } http://www.mta.ca/dmf/download/jme/imgp0520a.jpg } } My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My } feeling for typical vacuum system performance tells me it's the former, } but the labeling on the gauge is somewhat ambiguous. Does the large } "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems } I've used didn't leave this open to interpretation. } } Thanks in advance to those who respond and help dispel my uneasiness... } } Jim
==============================Original Headers============================== 4, 39 -- From benada-at-biomed.cas.cz Thu Oct 13 02:16:59 2011 4, 39 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 4, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9D7Gwvg022703 4, 39 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 02:16:59 -0500 4, 39 -- X-ASG-Debug-ID: 1318490209-011d8112ff54ed50001-4CH8be 4, 39 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id koANMKlg2KGd2pam (version=TLSv1 cipher=AES256-SHA bits=256 verify=NO); Thu, 13 Oct 2011 09:16:49 +0200 (CEST) 4, 39 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 4, 39 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 4, 39 -- Received: from u117ob.localnet (u117ob.mbu.cas.cz [147.231.44.101]) 4, 39 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 4, 39 -- (No client certificate requested) 4, 39 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id C39742C81FC; 4, 39 -- Thu, 13 Oct 2011 09:16:48 +0200 (CEST) 4, 39 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 4, 39 -- Organization: =?utf-8?q?Mikrobiologick=C3=BD_=C3=BAstav_AV?= =?utf-8?q?_=C4=8CR?= 4, 39 -- To: jehrman-at-mta.ca 4, 39 -- Subject: Re: [Microscopy] vacuum gauge reading 4, 39 -- Date: Thu, 13 Oct 2011 09:16:43 +0200 4, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] vacuum gauge reading 4, 39 -- User-Agent: KMail/1.13.7 (Linux/3.0.0-1-686-pae; KDE/4.6.5; i686; ; ) 4, 39 -- Cc: "Microscopy Listserver" {microscopy-at-microscopy.com} 4, 39 -- References: {201110121313.p9CDDNZn021928-at-ns.microscopy.com} 4, 39 -- In-Reply-To: {201110121313.p9CDDNZn021928-at-ns.microscopy.com} 4, 39 -- MIME-Version: 1.0 4, 39 -- Content-Type: Text/Plain; 4, 39 -- charset="iso-8859-2" 4, 39 -- Content-Transfer-Encoding: 7bit 4, 39 -- Message-Id: {201110130916.44261.benada-at-biomed.cas.cz} 4, 39 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 4, 39 -- X-Barracuda-Start-Time: 1318490209 4, 39 -- X-Barracuda-Encrypted: AES256-SHA 4, 39 -- X-Barracuda-URL: http://barracuda.biomed.cas.cz:8000/cgi-mod/mark.cgi 4, 39 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 4, 39 -- X-Barracuda-Spam-Score: 0.00 4, 39 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=1000.0 tests= 4, 39 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.77190 4, 39 -- Rule breakdown below 4, 39 -- pts rule name description 4, 39 -- ---- ---------------------- -------------------------------------------------- ==============================End of - Headers==============================
I would say the reading is between 1 x 10-6 and 1 x 10-5 so it has to be 2 x 10-6. If 10-6 actually stands for 10 x 10-6 somebody at Denton should be tarred and feathered:)
Philip
-----Original Message----- X-from: jehrman-at-mta.ca [mailto:jehrman-at-mta.ca] Sent: 12 October 2011 15:20 To: Philip Köck
Hi Listers,
This is a bit embarrassing, but I've been bothered by this long enough. Check out this picture of the vacuum gauge from my vacuum evaporator:
http://www.mta.ca/dmf/download/jme/imgp0520a.jpg
My question is: what reading is the gauge giving? ~2x10-5 or ~2x10-6? My feeling for typical vacuum system performance tells me it's the former, but the labeling on the gauge is somewhat ambiguous. Does the large "10-5" on the meter indicate 10x10-5, or 1x10-5? Older Denton systems I've used didn't leave this open to interpretation.
Thanks in advance to those who respond and help dispel my uneasiness...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Dear Listers, I inhereited a ASPEX PSEM 3025 from another department of our institution; where it was found abandoned; since the owner left the place for greener pastures. For around two years it has stayed in a corner of their lab. Everything looks fit and fine. I managed to power it on today!. The hitch is, that I do not have a operation manual or any kind of documentation. Could somebody having the same machine assist me in reviving this SEM. I would appreciate if a soft copy of its manual is mailed to me. What could be the current cost of this machine?
Mohammed Yousuf Ph.D Department of Mechanical Engineering Materials characterization Lab Texas A&M University at Qatar Doha, State of Qatar
==============================Original Headers============================== 3, 41 -- From mdyousuf-at-qu.edu.qa Thu Oct 13 05:09:18 2011 3, 41 -- Received: from mx2.qu.edu.qa (Mx2.qu.edu.qa [86.36.67.18]) 3, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DA9EIm027360 3, 41 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 05:09:17 -0500 3, 41 -- X-AuditID: 56244312-b7cbdae000007d89-e0-4e94a42fec24 3, 41 -- Received: from ITSMS85CAH3.qu.edu.qa (edge2.qu.edu.qa [86.36.69.139]) 3, 41 -- by mx2.qu.edu.qa (Qatar University Mail Server) with SMTP id 9E.18.32137.F24A49E4; Tue, 11 Oct 2011 23:16:47 +0300 (AST) 3, 41 -- Received: from ITSMS82MBX2.qu.edu.qa ([169.254.2.113]) by 3, 41 -- ITSMS85CAH3.qu.edu.qa ([86.36.69.139]) with mapi id 14.01.0339.001; Thu, 13 3, 41 -- Oct 2011 13:09:07 +0300 3, 41 -- From: Mohammed Yousuf {mdyousuf-at-qu.edu.qa} 3, 41 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 41 -- Subject: PSEM 3025 ASPEX - Need operation manual 3, 41 -- Thread-Topic: PSEM 3025 ASPEX - Need operation manual 3, 41 -- Thread-Index: AcyJkCSJ7QxEzztpQi+1/uqgMOuhUg== 3, 41 -- Date: Thu, 13 Oct 2011 10:09:06 +0000 3, 41 -- Message-ID: {EFD709C0BD166F4F8D2062B346C0C8DD20FB9C30-at-ITSMS82MBX2.qu.edu.qa} 3, 41 -- Accept-Language: en-US 3, 41 -- Content-Language: en-US 3, 41 -- X-MS-Has-Attach: 3, 41 -- X-MS-TNEF-Correlator: 3, 41 -- x-originating-ip: [86.36.69.151] 3, 41 -- Content-Type: text/plain; charset="us-ascii" 3, 41 -- MIME-Version: 1.0 3, 41 -- X-Brightmail-Tracker: H4sIAAAAAAAAA11TbUgTYQDu3W7ztL163nR72yzsUEizaWVQGhJEYSGrH2WRhZ3tdMPp9G6X 3, 41 -- HyTsRx80+6JMU4RczIKKPhHtQ7IJQdKH/qjAyH5oWCsLK1KorHt3m539e+553ue557njJdX0 3, 41 -- oygT6ah0c3wl62S0MURhyobGZZn+JmvWe++S1b/6PVHrQP5k40fVVrArZq2Nczr2c3xm3t4Y 3, 41 -- +8iXVlD1RlM7MTge5QEDhBeQJKKy0aebai+IlqABDY5c13pBDElTfQCN+Z+q5AcfQAOvWgA2 3, 41 -- aKl0dP9KKjYkULlo5vUFgLGeykLBSz+1Mr8KdU79UMnYgr43PdBgTFCpyNN7JwpjSBWgifGu 3, 41 -- 0HkgvXhq4GrovJoyouGx8yq5EIX895+HyyWiD6MzGhkno+C1ofD5DNRx76tWxkvRRd9HtZwf 3, 41 -- jx63jhGngL5NEdumsLQpLG0KSwcgLgNYUbvCUi1aOJtoqWZvgdCHXk/3gIOjGQFAkYDRweQ1 3, 41 -- TVZaw+4X6ioCYAupYhJhXk+zlY4tcdnq7KxgL+ZFJycwCZC+IdFwli4RneXMIkidlljjLCuI 3, 41 -- QpVjn8MlCsUi7wwARKol64oybLWxdfUc75IDA8BMEowRXn1bbaWpMtbNlXNcFcdH1J0kySBo 3, 41 -- wlXiea6Mqy11ON0RWfLl3pIUSqmECi2G2z+fsdImpfB/JxUZHQAFpE4qRuB8KFSxFYKjLJyt 3, 41 -- h/prEquLsKHchXAaDzVEyLmZA6DIZITT3bgSPmEXK2e7mgzQd1kS4hQCjjQlwxc5Z630AgU/ 3, 41 -- NzVyMYJgk/Sr9NCMy+qka/OvKw1TcNf5YTJUNQlO4aqJYW5uZlAarpKGZ/efxcPdrFs53Pzl 3, 41 -- DB4eZsPDF2LSECHnxpk8oERzZHexqYUtp4+N3LykGc85VBHrm/xzVLux8NzvaIOj+W5v2re0 3, 41 -- QXOPTrxrbPDVZDLOofGWRVONgdJtNbrPSXtexh1+klm4OTjP39WZVBpnK4ruc/z4eoBo8NfU 3, 41 -- 5z8jh1eq21uPvztR+lDfPV2e2x6kU25PenecjD/pWg5z7HUMIdjZ5elqXmD/AuOTRDZZBAAA 3, 41 -- Content-Transfer-Encoding: 8bit 3, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DA9EIm027360 ==============================End of - Headers==============================
So you can write it: 10-6 {10-5 {2.10-5 In this case how can 2x10-5 be situated between 10-5 and 10-6? And if the answer was 2x10-5, how could 8x10-5 be nearer to 10-5 than 2x10-5?? It is a simple question of logic!
Regards, Stephane
X-from: "colijn.1-at-osu.edu" {colijn.1-at-osu.edu} To: nizets2-at-yahoo.com Sent: Wednesday, October 12, 2011 11:03 PM
Hi all,
Like Ritchie, I am surprised at the multitude of responses and rationales for reading the pressure. The pressure reading is obvious just by looking at the scale. You don't need to think about which way the gauge moves or how things pump down. These are just red herrings!
The gauge is a PRESSURE gauge. It has an obvious logarithmic scale with higher pressures to the right. The numbers between 10-6 and 10-5 are the pressures between 1x10-6 and 1x10-5 (= 10x10-6).
The pressure is approx. 2x10-6 (or to coin a unit... 2 microtorr )
{/ on soapbox/} There is no doubt (and should be a very short discussion!) {/off soapbox/}
Cheers, Henk
At 10/12/2011 4:38 PM, r.sims-at-auckland.ac.nz wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I can hardly believe that this is even debateable! } } The gauge measures pressure (even though sub-atmospheric pressures are also called "vacuums"). } } Higher pressures are to the RHS, thus a pressure of 10exp-3 is a higher pressure than one of 10exp-9. } } So, as the pressure decreases from 10exp-5, it goes: 1x10exp-5, 0.8x10exp-5 (ie 8x10exp-6), 0.6x10exp-5 (ie 6x10exp-6) etc etc. } } I can't see that it is indicating anything but a smidgen above (pressure!) 0.2x10exp-5, ie 2x10exp-6, but maybe I'm just not seeing the problem clearly. } } cheers } Ritchie Sims } University of Auckland } } } } } ________________________________________ } X-from: kenconverse-at-qualityimages.biz [kenconverse-at-qualityimages.biz] } Sent: 13 October 2011 08:13 } To: Ritchie Sims } Subject: [Microscopy] RE: FW: Vacuum gauge reading } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Roger just said everything I was going to say. If Denton did this } correctly, the gauge is reading 2x10E-6 (slightly higher pressure than } 1x10E-6 to its left). If they screwed up the calibration, shame on them. } Being a cold cathode gauge it may be in need of both cleaning and } calibrating. } } In my experience if the 1x10E-5 was to indicate the range (as on some Varian } gauges), then it would have been placed roughly over the 4 in the range that } was intended. If it is placed on the end of a range, it indicates 1x10E-? } } My $.02 } } Ken Converse } owner } } QUALITY IMAGES } Servicing Scanning Electron Microscopes } Since 1981 } 474 So. Bridgton Rd. } Bridgton, ME 04009 } 207-647-4348 } kenconverse-at-qualityimages.biz } qualityimages.biz } } } -----Original Message----- } X-from: raristau-at-ims.uconn.edu [mailto:raristau-at-ims.uconn.edu] } Sent: Wednesday, October 12, 2011 2:32 PM } To: kenconverse-at-qualityimages.biz } Subject: [Microscopy] FW: Vacuum gauge reading } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } OK here comes Roger v1.3... } } I admit to being a little blindsided by Jim's original posting, but I hope } no harm was done by my erring on the side of "didaction for dummies". Excuse } me if I did. My excuse: I am accustomed to educating graduate students. } } Jim, if your sense is that the time of pumping was insufficient to reach } 10e-6 range, therefore the meter MUST be displaying 2 x 10e-5, perhaps there } is an issue of inaccurate, uncalibrated, or otherwise deficient gauges. (Or } a really fast pump.) Most thermocouple and ionizing gauges will become } inaccurate through buildup of surface deposits on the gauge. Also they are } calibrated for specific gases; a different gas composition will result in an } inaccurate gauge. } } All my money is still on 2 x 10e-6. HOWEVER, one caveat: If we get word from } the maker of the meter and they meant 10e-6 = 10 x 10e-6, then I withdraw my } wager. But I would still insist that is an improper way to label the meter. } } Still cheerful } Roger } } } ------ Forwarded Message } X-from: Roger Ristau {raristau-at-ims.uconn.edu} } Date: Wed, 12 Oct 2011 13:48:48 -0400 } To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } Conversation: [Microscopy] Vacuum gauge reading } Subject: FW: [Microscopy] Vacuum gauge reading } } A follow-up on my previous message: (I can't get enough "out-of-office" } replies!) } } A tic marked 10e-6 means 1 x 10e-6. } It makes no sense for it to represent 10 x 10e-6; that is an "improper } fraction". } } Cheers all } Roger } } ------ Forwarded Message } X-from: {raristau-at-ims.uconn.edu} } Reply-To: {raristau-at-ims.uconn.edu} } Date: Wed, 12 Oct 2011 11:53:12 -0500 } To: {raristau-at-ims.uconn.edu} } Subject: [Microscopy] Vacuum gauge reading } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This has been a fun thread to follow.... } } With all humility and respect, it is plain that Jim is not the only one } confused by these gauges. I have stared at a meter for many minutes many } times as I tried to sort this out in my own head. } } Vacuum gauges, whether measuring current, voltage, resistance, etc. are just } like most other meters: The lower, or smaller, or least magnitude values are } on the left, the larger on the right. It is just that the pressure gauge } starts with the needle pointing to the larger value on the right (closer to } atmospheric pressure), and moves to the smaller value end of the meter on } the left as the pumps improve the vacuum. We are more accustomed to most } other sorts of gauges in which the needle starts by pointing to the smaller } magnitude on the left and moves left to right as the gadget it is attached } to does its stuff. } } Therefore, the vacuum meter reading, as the needle moves to the left of the } tic that is marked 10e-5 (= 1 x 10e-5), has reached the 10e-6 range. Note } that the scale shows 8 (x 10e-6) to the right of 6 (x 10e-6), that is, the } larger magnitude to the right of the smaller magnitude. The meter in Jim's } image is reading just a hair higher than 2 x 10e-6. } } Of course, not content to leave well enough alone, we create most of our own } confusion when we refer to "higher vacuum" as the state when the magnitude } of pressure is "lower", and "lower vacuum" when the pressure reading is of } "higher" magnitude. But that is another thread... } } With smiles } Roger } } Roger A. Ristau, PhD } Electron Microscopy Specialist } Institute of Materials Science } 97 North Eagleville Road } University of Connecticut } Storrs, CT 06269 } vox: 860-486-5453 } fax: 860-486-4745 } } } } } ==============================Original Headers============================== } 9, 21 -- From raristau-at-ims.uconn.edu Wed Oct 12 11:50:51 2011 } 9, 21 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu } [137.99.25.235]) } 9, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p9CGopcD011522 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 11:50:51 -0500 } 9, 21 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu } [137.99.20.184]) } 9, 21 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id 1F7FC2CE43 } 9, 21 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 12:50:51 -0400 } (EDT) } 9, 21 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 9, 21 -- Date: Wed, 12 Oct 2011 12:50:50 -0400 } 9, 21 -- Subject: Vacuum gauge reading } 9, 21 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } 9, 21 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } 9, 21 -- Message-ID: {CABB3DAA.3200%raristau-at-ims.uconn.edu} } 9, 21 -- Thread-Topic: Vacuum gauge reading } 9, 21 -- Thread-Index: AcyI/xi9VyGf0vTyEeCRxwAbY55CVA== } 9, 21 -- Mime-version: 1.0 } 9, 21 -- Content-type: text/plain; } 9, 21 -- charset="US-ASCII" } 9, 21 -- Content-transfer-encoding: 7bit } 9, 21 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 } 9, 21 -- X-Virus-Status: Clean } ==============================End of - Headers============================== } } ------ End of Forwarded Message } } ------ End of Forwarded Message } } } } ==============================Original Headers============================== } 28, 22 -- From raristau-at-ims.uconn.edu Wed Oct 12 13:29:03 2011 } 28, 22 -- Received: from mta2.uits.uconn.edu (mta2.uits.uconn.edu } [137.99.25.235]) } 28, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } p9CIT3Qf004544 } 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 13:29:03 } -0500 } 28, 22 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu } [137.99.20.184]) } 28, 22 -- by mta2.uits.uconn.edu (Postfix) with ESMTP id DFD4C2CFCA } 28, 22 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:29:02 } -0400 (EDT) } 28, 22 -- User-Agent: Microsoft-Entourage/11.4.0.080122 } 28, 22 -- Date: Wed, 12 Oct 2011 14:29:01 -0400 } 28, 22 -- Subject: FW: [Microscopy] Vacuum gauge reading } 28, 22 -- From: Roger Ristau {raristau-at-ims.uconn.edu} } 28, 22 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} } 28, 22 -- Message-ID: {CABB54AD.3222%raristau-at-ims.uconn.edu} } 28, 22 -- Thread-Topic: [Microscopy] Vacuum gauge reading } 28, 22 -- Thread-Index: AcyJBzHKcDslHPT6EeCBZAAbY55CVAABZ5DK } 28, 22 -- In-Reply-To: {CABB4B40.321B%raristau-at-ims.uconn.edu} } 28, 22 -- Mime-version: 1.0 } 28, 22 -- Content-type: text/plain; } 28, 22 -- charset="US-ASCII" } 28, 22 -- Content-transfer-encoding: 7bit } 28, 22 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta2 } 28, 22 -- X-Virus-Status: Clean } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 40, 28 -- From kenconverse-at-qualityimages.biz Wed Oct 12 14:12:27 2011 } 40, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) } 40, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CJCRaS002197 } 40, 28 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 14:12:27 -0500 } 40, 28 -- X-Authority-Analysis: v=2.0 cv=O6u7TWBW c=1 sm=0 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=a28-TuC6_4MA:10 a=kj9zAlcOel0A:10 a=Zx37jsudAAAA:8 a=MKxSoT7or7n8rsCZ3E0A:9 a=MjCJU8d-ybvQtNNfEm4A:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=ZQ0-ODaO5YQA:10 a=L7AOxdDgvMIA:10 a=RyjLG6jaqdoA:10 a=G91thX30KR8A:10 a=ZC8Yv9kwYYWqhca4:21 a=CTP1pKWpgRALcfUy:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 } 40, 28 -- X-Cloudmark-Score: 0 } 40, 28 -- X-Originating-IP: 72.224.149.57 } 40, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2423] helo=Ken) } 40, 28 -- by cdptpa-oedge01.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) } 40, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP } 40, 28 -- id C3/0A-06600-A96E59E4; Wed, 12 Oct 2011 19:12:27 +0000 } 40, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} } 40, 28 -- To: {raristau-at-ims.uconn.edu} , "MSA Listserver" {microscopy-at-microscopy.com} } 40, 28 -- Subject: RE: [Microscopy] FW: Vacuum gauge reading } 40, 28 -- Date: Wed, 12 Oct 2011 15:12:20 -0400 } 40, 28 -- Message-ID: {656CA10E51FE480792DFFCCF028FA6B3-at-Ken} } 40, 28 -- MIME-Version: 1.0 } 40, 28 -- Content-Type: text/plain; } 40, 28 -- charset="us-ascii" } 40, 28 -- X-Priority: 3 (Normal) } 40, 28 -- X-MSMail-Priority: Normal } 40, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 } 40, 28 -- In-Reply-To: {201110121831.p9CIVpWw015451-at-ns.microscopy.com} } 40, 28 -- Importance: Normal } 40, 28 -- Thread-Index: AcyJDTQvEHHpw1XfTzyWCbC6rI/RigABLkiw } 40, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 } 40, 28 -- Content-Transfer-Encoding: 8bit } 40, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CJCRaS002197 } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 57, 51 -- From r.sims-at-auckland.ac.nz Wed Oct 12 15:37:14 2011 } 57, 51 -- Received: from mx1.auckland.ac.nz (mx1.auckland.ac.nz [130.216.12.42]) } 57, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CKbA9k020103 } 57, 51 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 15:37:13 -0500 } 57, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; } 57, 51 -- d=auckland.ac.nz; i=r.sims-at-auckland.ac.nz; q=dns/txt; } 57, 51 -- s=uoa; t=1318451833; x=1349987833; } 57, 51 -- h=from:to:subject:date:message-id:references:in-reply-to: } 57, 51 -- content-transfer-encoding:mime-version; } 57, 51 -- z=From:=20Ritchie=20Sims=20 {r.sims-at-auckland.ac.nz} |To:=20" } 57, 51 -- microscopy-at-microscopy.com"=20 {microscopy-at-microscopy.com} } 57, 51 -- |Subject:=20RE:=20[Microscopy]=20RE:=20FW:=20Vacuum=20gau } 57, 51 -- ge=20reading|Date:=20Wed,=2012=20Oct=202011=2020:37:08=20 } 57, 51 -- +0000|Message-ID:=20 {65F9DBC3DFB6DE44BE7695B82D539F1A1E2E } 57, 51 -- 5466-at-uxcn10-1.UoA.auckland.ac.nz} |References:=20 {20111012 } 57, 51 -- 1913.p9CJDAAn003326-at-ns.microscopy.com} |In-Reply-To:=20 {20 } 57, 51 -- 1110121913.p9CJDAAn003326-at-ns.microscopy.com} } 57, 51 -- |Content-Transfer-Encoding:=20quoted-printable } 57, 51 -- |MIME-Version:=201.0; } 57, 51 -- bh=LehGCDvCVGAn953JQ2RBlkT0GfSh8/8XdcjaxaFFNCI=; } 57, 51 -- b=SbYW6y40G6L7sqBgt71/dAfEArbKeaqB9x0t7NqNYLinq7SXflF4VRxT } 57, 51 -- aerEib3VGlMmGFJt/DriIM0ea0to68GeWzdTnnlNNWEdDCw7pO443vNQ1 } 57, 51 -- kTxLBJTmhNCHqa6dLp1rXkSwK7jVc4iWxT2NDQz+gjyHyjjWil2jRsi9O } 57, 51 -- k=; } 57, 51 -- X-IronPort-AV: E=Sophos;i="4.69,336,1315137600"; } 57, 51 -- d="scan'208";a="106294466" } 57, 51 -- X-Ironport-HAT: MAIL-SERVERS - $RELAYED } 57, 51 -- X-Ironport-Source: 130.216.4.106 - Outgoing - Outgoing } 57, 51 -- Received: from uxchange10-fe2.itsys.auckland.ac.nz (HELO uxchange10-fe2.UoA.auckland.ac.nz) ([130.216.4.106]) } 57, 51 -- by mx1-int.auckland.ac.nz with ESMTP/TLS/AES128-SHA; 13 Oct 2011 09:37:08 +1300 } 57, 51 -- Received: from UXCN10-1.UoA.auckland.ac.nz ([169.254.1.62]) by } 57, 51 -- uxchange10-fe2.UoA.auckland.ac.nz ([130.216.4.106]) with mapi id } 57, 51 -- 14.01.0289.001; Thu, 13 Oct 2011 09:37:08 +1300 } 57, 51 -- From: Ritchie Sims {r.sims-at-auckland.ac.nz} } 57, 51 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 57, 51 -- Subject: RE: [Microscopy] RE: FW: Vacuum gauge reading } 57, 51 -- Thread-Topic: [Microscopy] RE: FW: Vacuum gauge reading } 57, 51 -- Thread-Index: AQHMiRL9WURKj4XdpkSfOkAwP4r5CJV5Jz5f } 57, 51 -- Date: Wed, 12 Oct 2011 20:37:08 +0000 } 57, 51 -- Message-ID: {65F9DBC3DFB6DE44BE7695B82D539F1A1E2E5466-at-uxcn10-1.UoA.auckland.ac.nz} } 57, 51 -- References: {201110121913.p9CJDAAn003326-at-ns.microscopy.com} } 57, 51 -- In-Reply-To: {201110121913.p9CJDAAn003326-at-ns.microscopy.com} } 57, 51 -- Accept-Language: en-GB, en-NZ, en-US } 57, 51 -- Content-Language: en-GB } 57, 51 -- X-MS-Has-Attach: } 57, 51 -- X-MS-TNEF-Correlator: } 57, 51 -- x-originating-ip: [130.216.11.103] } 57, 51 -- Content-Type: text/plain; charset="us-ascii" } 57, 51 -- MIME-Version: 1.0 } 57, 51 -- Content-Transfer-Encoding: 8bit } 57, 51 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9CKbA9k020103 } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 10, 26 -- From colijn.1-at-osu.edu Wed Oct 12 15:59:37 2011 10, 26 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9CKxbDC004509 10, 26 -- for {microscopy-at-microscopy.com} ; Wed, 12 Oct 2011 15:59:37 -0500 10, 26 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 26 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 26 -- id {01O74Y6Y2DA892XO6X-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 10, 26 -- Wed, 12 Oct 2011 16:59:35 -0400 (EDT) 10, 26 -- Received: from [164.107.76.202] (hoc3.ceof.ohio-state.edu [164.107.76.202]) 10, 26 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 26 -- with ESMTPA id {01O74Y6XKIP493YOD3-at-ecr6.ohio-state.edu} ; Wed, 10, 26 -- 12 Oct 2011 16:59:34 -0400 (EDT) 10, 26 -- Date: Wed, 12 Oct 2011 16:59:34 -0400 10, 26 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 26 -- Subject: Re: [Microscopy] FW: Vacuum gauge reading 10, 26 -- In-reply-to: {201110122038.p9CKcUgZ021962-at-ns.microscopy.com} 10, 26 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 26 -- To: r.sims-at-auckland.ac.nz, microscopy-at-microscopy.com 10, 26 -- Message-id: {4E95FFB6.5050900-at-osu.edu} 10, 26 -- MIME-version: 1.0 10, 26 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 10, 26 -- Content-transfer-encoding: 7bit 10, 26 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 10, 26 -- Thunderbird/7.0.1 10, 26 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 26 -- References: {201110122038.p9CKcUgZ021962-at-ns.microscopy.com} ==============================End of - Headers==============================
==============================Original Headers============================== 18, 45 -- From nizets2-at-yahoo.com Thu Oct 13 06:08:58 2011 18, 45 -- Received: from nm25.bullet.mail.sp2.yahoo.com (nm25.bullet.mail.sp2.yahoo.com [98.139.91.95]) 18, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9DB8s4Y012561 18, 45 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 06:08:55 -0500 18, 45 -- Received: from [98.139.91.67] by nm25.bullet.mail.sp2.yahoo.com with NNFMP; 13 Oct 2011 11:08:51 -0000 18, 45 -- Received: from [98.139.91.42] by tm7.bullet.mail.sp2.yahoo.com with NNFMP; 13 Oct 2011 11:08:51 -0000 18, 45 -- Received: from [127.0.0.1] by omp1042.mail.sp2.yahoo.com with NNFMP; 13 Oct 2011 11:08:51 -0000 18, 45 -- X-Yahoo-Newman-Property: ymail-3 18, 45 -- X-Yahoo-Newman-Id: 518165.44858.bm-at-omp1042.mail.sp2.yahoo.com 18, 45 -- Received: (qmail 64715 invoked by uid 60001); 13 Oct 2011 11:08:51 -0000 18, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1318504131; bh=iHhS452fE55eScX0psVJSna8u1/aT48y30yZO6PBy2M=; h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=mOrNWcjN7CGwD+7S1AjfUHuqhjB4t2gtWcU6bYxAxoq5C/94Nv9qlEXdth608yYi5dJ0VWKFaclIMNoxf9DVZ8WBUr0UE54uZ1tYdiYIEMHSucMCXt0aYZCyArsklQt1o4jl7KlycCgnsx3LFgHFBJQvRzT9dzST7cy+1Rdbeps= 18, 45 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 18, 45 -- s=s1024; d=yahoo.com; 18, 45 -- h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 18, 45 -- b=kHIMh8PH8EbhRMMxkQRI4gi3BtuGuSjoPTDINVOqmb5mLfjceakE4QtG9MmkJaot3IRc/yUfPX4t3mP98DmceOnMT2xRMrLUJtjllUCL76aQfHoG6oUsuTHoNvqdNlNbDGuu2j/qU7+M2gg0azb95k27kM1c7DhqNDJ/3gjGKoM=; 18, 45 -- X-YMail-OSG: d6rjL9UVM1lKjqzNs7GmagpAELmRaqCLMGMy7zCBa51XPkj 18, 45 -- FKoi5CQMRcpBvfZdq8egPOK5I_h3rcmLoUPX1S5DkKZREU1fZOH6NClOSoUt 18, 45 -- OQLemUK3jImaCCPbSxhGYtBp8y6vQ8yrdi3BznzQY4xaSdg.UGOF4NlcCx.Q 18, 45 -- 8Rc26BJ60NP3S_6VyZa54LLb6vFWxIziEKgjF9OqSvK0Sa19czzwjS.vPX9N 18, 45 -- MR_Qxl77tU92rSQlMy.Avhep8TNc5sSdxmAg7WfwuuaYTwAdKguhl8Q6uis2 18, 45 -- _ElkxtSOlyaD1udGrOtgVhOl_jRBS2n16Ovvm6UXVUrURfQnFb2cf4DhwG6N 18, 45 -- SLDWVrnf5r4HIDs2xOBG_42gZiSbuuiJkyWf3GvWJuymsV8T07HoX_M6mQ33 18, 45 -- nPV3tB0GcAPvNzw0VTxwCguy2FAqRw13dt3Uu.Fkxj7lITH2HZUHoRR96b3a 18, 45 -- PVF_FsX_vKo3nSJ3HtGcU2ueSKvIe0UCtWRh84rhG8V4soj8hoXTf_Zvsmc2 18, 45 -- noJ.l13rTvvPiNolu9hSVpow9Rz7qR_FHLFEITge0IdKqBQNwiNZSRtrMX0f 18, 45 -- IYeUIIpBqyR9h7IhMt_bWs.z07xziXtspijBuRmwI.q.YOza3lE0ZIZosJNr 18, 45 -- 735kWRwUpZlvI9UjE4VKfbYiKYroj8GreFB4m6C9Rm5_e5Z2wqVaK1NCUF2W 18, 45 -- duyhDn0CoG6DP3OuSqYAP_lt_x2szEoGKMFRgcjTGh9k9_9Ip2rOxARvv_MJ 18, 45 -- PRGloauIAvknfZH56r6U2cIaJHGlPWiD5OoNHnOMqj3vp3B_Mku8i0x84C3b 18, 45 -- 1cSWpR01mei57pE0.3.eeuXZ9plp_AxQY5YkmWECeWXVPVA6VASjc_1d4ml6 18, 45 -- a2clwqpvSeZAXWyCVkwDLHdXt3i_aQnc45azcqwNihLfumnzybzq8bw-- 18, 45 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Thu, 13 Oct 2011 04:08:50 PDT 18, 45 -- X-Mailer: YahooMailWebService/0.8.114.317681 18, 45 -- References: {201110122103.p9CL3QIV010524-at-ns.microscopy.com} 18, 45 -- Message-ID: {1318504130.52912.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} 18, 45 -- Date: Thu, 13 Oct 2011 04:08:50 -0700 (PDT) 18, 45 -- From: Stephane Nizet {nizets2-at-yahoo.com} 18, 45 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 18, 45 -- Subject: Re: [Microscopy] Re: FW: Vacuum gauge reading 18, 45 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 18, 45 -- In-Reply-To: {201110122103.p9CL3QIV010524-at-ns.microscopy.com} 18, 45 -- MIME-Version: 1.0 18, 45 -- Content-Type: text/plain; charset=iso-8859-1 18, 45 -- Content-Transfer-Encoding: 8bit 18, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DB8s4Y012561 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jman-at-u.northwestern.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jman-at-u.northwestern.edu Name: Jason Mantei
Organization: Northwestern University
Title-Subject: [Filtered] vacuum gauge reading
Message: It has to be 2x10-6:
10^-6 is .000001. 10^-5 is .00001. The needle on the gauge is between these two values, so 2x10^-5, or .00002, doesn't make sense because it's outside of the range.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both alberto.diaspro-at-iit.it as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: alberto.diaspro-at-iit.it Name: Alberto Diaspro
My advice is to get a vacuum gauge with a digital readout!
John Bozzola Reformed user of slide rules ....... anyone remember those?
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 5, 20 -- From bozzola-at-siu.edu Thu Oct 13 10:27:48 2011 5, 20 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 5, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DFRmm0014425 5, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 10:27:48 -0500 5, 20 -- Received: by vws16 with SMTP id 16so179749vws.0 5, 20 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 08:27:47 -0700 (PDT) 5, 20 -- MIME-Version: 1.0 5, 20 -- Received: by 10.52.96.161 with SMTP id dt1mr4305510vdb.124.1318519667623; Thu, 5, 20 -- 13 Oct 2011 08:27:47 -0700 (PDT) 5, 20 -- Received: by 10.52.109.169 with HTTP; Thu, 13 Oct 2011 08:27:47 -0700 (PDT) 5, 20 -- In-Reply-To: {201110131521.p9DFLegm003371-at-ns.microscopy.com} 5, 20 -- References: {201110131521.p9DFLegm003371-at-ns.microscopy.com} 5, 20 -- Date: Thu, 13 Oct 2011 10:27:47 -0500 5, 20 -- Message-ID: {CAKeCJ9wt4tV1gzboXWVQs2d6bON+7-74LDhdPDf-8S-0J20KYw-at-mail.gmail.com} 5, 20 -- Subject: Re: [Microscopy] viaWWW:vacuum gauge reading 5, 20 -- From: John Bozzola {bozzola-at-siu.edu} 5, 20 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 20 -- Content-Type: text/plain; charset=UTF-8 5, 20 -- Content-Transfer-Encoding: 8bit 5, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DFRmm0014425 ==============================End of - Headers==============================
James The gauge is reading 2X10-6 I hope this helps
Thank You George Mehaffey Field Service Manager Main # 856-439-9100 Desk # 856-380-5210 Cell # 609-304-9831
My inborn gauge reading skills are gloating, my intuition regarding vacuum systems is depressed. Now I must either have a stunningly high performance vacuum system, or a significantly miscalibrated gauge. Whoopee!
Next time I'll try to post a less contentious question. For example: my Denton CPD is the only commercial CPD I'm aware of that doesn't have a viewing window. Good or bad thing? :-)
As usual, thanks to all who took the time to post a reply. I apologize for any increases in blood pressure as a result.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Hi, all I would read vacuum gauge from higher (right) to lower pressure (left). The reason is 1) that there is no zero pressure (infinite lower, but no zero) on the left end, but there is a finite on higher pressure (on right side), the 760 torr, and 2) the gauge starts showing pressure change from the higher end when we start pumping, which tells us the way we should follow to read its changes. Instrument does not start pumping down from lower pressure (left side), this is also the reason we should not read it from left side. As instrument pumps down, It shows 760 torr-1torr, 1E-1, then 0.1xE-1 (1xE-2), then 0.01xE-1 (1xE-3), 0.001xE-1 (1xE-4), 0.0001xE-1 (1xE-5), 0.00001xE-1 (1xE-6) and so on. Notice that I use the same unit for all readings, the xE-1, but actual unit on gauge panel is different.
Let's make a simple case. If the needle is located between 760Torr and 1Torr, we know the instrument just starts pumping down and we would read it by counting down from 760 (higher) to 1 torr (lower), no other way around. For the same reason for reading, if the needle is located between 1xE-6 and 1xE-5, we will need to read it from higher to lower (right to left). As long as needle stays above 1xE-6 (right side of xE-6 mark), it is on xE-5 territory and any reading should have a unit of xE-5, not xE-6. At any given time we will read a needle position somewhere on the panel, and we know instrument is still pumping down to keep the pressure down from that number, so we need to read the number and report it with its unit by counting down from the nearest highest pressure unit, from right to left in this case, on the vacuum gauge.
Thanks, Vincent Schafer Vallecitos Laboratory Sunol, CA
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Thursday, October 13, 2011 8:38 AM To: vwang-at-schaferlabs.com
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jman-at-u.northwestern.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jman-at-u.northwestern.edu Name: Jason Mantei
Organization: Northwestern University
Title-Subject: [Filtered] vacuum gauge reading
Message: It has to be 2x10-6:
10^-6 is .000001. 10^-5 is .00001. The needle on the gauge is between these two values, so 2x10^-5, or .00002, doesn't make sense because it's outside of the range.
Bad thing. You don't get to show people the pretty purple argon glow and then rant on how real neon signs are made, instead of these cheap phosphor-coating-inside-the-tube imitations that are so common now.
(And with no window, you can't check for the horrid white or blue glow of air not flushed out by the argon.)
Phil
} Next time I'll try to post a less contentious question. For example: } my Denton CPD is the only commercial CPD I'm aware of that doesn't } have a viewing window. Good or bad thing? :-) } } As usual, thanks to all who took the time to post a reply. I } apologize for any increases in blood pressure as a result. } } Jim } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 26 -- From oshel1pe-at-cmich.edu Thu Oct 13 11:27:18 2011 6, 26 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DGRHqx013583 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 11:27:17 -0500 6, 26 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 6, 26 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p9DGRGo0019848 6, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 12:27:17 -0400 6, 26 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 6, 26 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Thu, 13 Oct 6, 26 -- 2011 12:27:16 -0400 6, 26 -- MIME-Version: 1.0 6, 26 -- Message-ID: {a0624080fcabcc0e277fc-at-[141.209.160.249]} 6, 26 -- In-Reply-To: {201110131611.p9DGBkrM024343-at-ns.microscopy.com} 6, 26 -- References: {201110131611.p9DGBkrM024343-at-ns.microscopy.com} 6, 26 -- Date: Thu, 13 Oct 2011 12:27:14 -0400 6, 26 -- To: {Microscopy-at-microscopy.com} 6, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 26 -- Subject: Re: [Microscopy] vacuum gauge reading - resolved (?) 6, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 26 -- X-Originating-IP: [141.209.160.249] 6, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 26 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 6, 26 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 26 -- X-CanItPRO-Stream: default 6, 26 -- X-Canit-Stats-ID: 02FIgrgaH - abaad3b80a1b - 20111013 6, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Phil (And collecting new kinds of "out of office" autoreplies.)
} Phil, } } Stop messing with my mind! Argon glow from a critical point dryer? I } thought I was adrift when a simple reading on a vacuum gauge caused } such a stir. } } But your response also tells me how quickly a posting can run off } into the weeds.... } } Jim } } } On 13/10/2011 1:28 PM, oshel1pe-at-cmich.edu wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Jim, } } } } Bad thing. You don't get to show people the pretty purple argon glow } } and then rant on how real neon signs are made, instead of these cheap } } phosphor-coating-inside-the-tube imitations that are so common now. } } } } (And with no window, you can't check for the horrid white or blue } } glow of air not flushed out by the argon.) } } } } Phil } } } } } Next time I'll try to post a less contentious question. For example: } } } my Denton CPD is the only commercial CPD I'm aware of that doesn't } } } have a viewing window. Good or bad thing? :-) } } } } } } As usual, thanks to all who took the time to post a reply. I } } } apologize for any increases in blood pressure as a result. } } } } } } Jim } } } -- } } } } } } James M. Ehrman } } } Digital Microscopy Facility } } } Mount Allison University } } } 63B York St. } } } Sackville, NB E4L 1G7 } } } CANADA } } } } } } phone: 506-364-2519 } } } fax: 506-364-2505 } } } email: jehrman-at-mta.ca } } } www: http://www.mta.ca/dmf } } } -- } } James M. Ehrman } Digital Microscopy Facility } Mount Allison University } 63B York St. } Sackville, NB E4L 1G7 } CANADA } } phone: 506-364-2519 } fax: 506-364-2505 } email: jehrman-at-mta.ca } www: http://www.mta.ca/dmf } } If you give some managers an inch they think they're a ruler.
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 6, 27 -- From oshel1pe-at-cmich.edu Thu Oct 13 11:55:23 2011 6, 27 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 6, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DGtMRY005001 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 11:55:22 -0500 6, 27 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 6, 27 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p9DGbrJ7026957 6, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 12:37:54 -0400 6, 27 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 6, 27 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Thu, 13 Oct 6, 27 -- 2011 12:55:21 -0400 6, 27 -- MIME-Version: 1.0 6, 27 -- Message-ID: {a06240810cabcc78c07f4-at-[141.209.160.249]} 6, 27 -- In-Reply-To: {4E971316.1050301-at-mta.ca} 6, 27 -- References: {201110131628.p9DGS0xB014780-at-ns.microscopy.com} 6, 27 -- {4E971316.1050301-at-mta.ca} 6, 27 -- Date: Thu, 13 Oct 2011 12:55:20 -0400 6, 27 -- To: {Microscopy-at-microscopy.com} 6, 27 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 27 -- Subject: Re: [Microscopy] Re: vacuum gauge reading - resolved (?) 6, 27 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 6, 27 -- X-Originating-IP: [141.209.160.249] 6, 27 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 6, 27 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 6, 27 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 6, 27 -- X-CanItPRO-Stream: default 6, 27 -- X-Canit-Stats-ID: 03FIgBS6G - 7635698e7767 - 20111013 6, 27 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
If anyone might be interested in acquiring our Cambridge S200 SEM and Orion imaging system, please contact me off-list at the address below.
Regards,
Paul Callomon Collections Manager in Malacology, Invertebrate Paleontology and General Invertebrates Academy of Natural Sciences 1900 Parkway, Philadelphia, PA 19103-1195, USA callomon-at-ansp.org Tel. 215-405-5096
==============================Original Headers============================== 6, 21 -- From callomon-at-ansp.org Thu Oct 13 13:04:33 2011 6, 21 -- Received: from mail.ansp.org (mail.ansp.org [192.204.19.65]) 6, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DI4XXt022768 6, 21 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 13:04:33 -0500 6, 21 -- Received: from mail.ansp.org ([fe80::b55d:262c:e9e6:c197]) by mail.ansp.org 6, 21 -- ([fe80::b55d:262c:e9e6:c197%11]) with mapi; Thu, 13 Oct 2011 14:05:42 -0400 6, 21 -- From: Paul Callomon {callomon-at-ansp.org} 6, 21 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 6, 21 -- Subject: Cambridge S200 SEM 6, 21 -- Thread-Topic: Cambridge S200 SEM 6, 21 -- Thread-Index: AcyJ0rxpJOFLXiQ7QHiWfOs98Ut0OQ== 6, 21 -- Date: Thu, 13 Oct 2011 18:05:42 +0000 6, 21 -- Message-ID: {A239B1CB55E7924E955AB07041E7185926D14165-at-mail.ansp.org} 6, 21 -- Accept-Language: en-US 6, 21 -- Content-Language: en-US 6, 21 -- X-MS-Has-Attach: 6, 21 -- X-MS-TNEF-Correlator: 6, 21 -- Content-Type: text/plain; charset="us-ascii" 6, 21 -- MIME-Version: 1.0 6, 21 -- Content-Transfer-Encoding: 8bit 6, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DI4XXt022768 ==============================End of - Headers==============================
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/13/2011 12:10 PM, jehrman-at-mta.ca wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Denton's response: } } James } The gauge is reading 2X10-6 I hope this helps } } Thank You } George Mehaffey } Field Service Manager } Main # 856-439-9100 } Desk # 856-380-5210 } Cell # 609-304-9831 } } My inborn gauge reading skills are gloating, my intuition regarding vacuum systems is depressed. Now I must either have a stunningly high performance vacuum system, or a significantly miscalibrated gauge. Whoopee! } } Next time I'll try to post a less contentious question. For example: my Denton CPD is the only commercial CPD I'm aware of that doesn't have a viewing window. Good or bad thing? :-) } } As usual, thanks to all who took the time to post a reply. I apologize for any increases in blood pressure as a result. } } Jim } }
==============================Original Headers============================== 4, 40 -- From vray-at-partbeamsystech.com Thu Oct 13 15:44:58 2011 4, 40 -- Received: from nm24.bullet.mail.sp2.yahoo.com (nm24.bullet.mail.sp2.yahoo.com [98.139.91.94]) 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9DKivqj009371 4, 40 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 15:44:58 -0500 4, 40 -- Received: from [98.139.91.68] by nm24.bullet.mail.sp2.yahoo.com with NNFMP; 13 Oct 2011 20:44:57 -0000 4, 40 -- Received: from [98.139.91.4] by tm8.bullet.mail.sp2.yahoo.com with NNFMP; 13 Oct 2011 20:44:57 -0000 4, 40 -- Received: from [127.0.0.1] by omp1004.mail.sp2.yahoo.com with NNFMP; 13 Oct 2011 20:44:57 -0000 4, 40 -- X-Yahoo-Newman-Id: 369482.5283.bm-at-omp1004.mail.sp2.yahoo.com 4, 40 -- Received: (qmail 5346 invoked from network); 13 Oct 2011 20:44:56 -0000 4, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1318538696; bh=FD0ulqennunVzjisbl5K1ZGe0sJvJf7e6VZAUWZsC7U=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=puao4d/xoE9ncgrTYGc6pGEcMoMwzWHRJdzEsfQHSTED3iNdjeiY3D5T8sTx2e1lTBtPvyb/YnsqrSD5y2s7KsnPr0rozdq4yhtyPoUtUk7HjW/yPNr3Y6c5y3kunLV6T/FzD7CJBZSJFOH+vKlpVXScfjeHXaFKRoEKOpZyWYs= 4, 40 -- X-Yahoo-Newman-Property: ymail-3 4, 40 -- X-YMail-OSG: bbfR2ykVM1lYLKX73AlwjHuGhNHy_ZQY9kUepKCL0udtpbs 4, 40 -- KWIQ8qjS9sCq8XA7zOanJUa0CbvG.ZuqELZDOxZ9oRI1UYZEelOWTT4_caHP 4, 40 -- 0EjurhIHov.Fd08Km5t0eFx9YKX5oR1L5vIu6yAw8.gzuAxKO1_JGIVw0ZKG 4, 40 -- 8F9qvCnj7pcj7ddlDAeukXS71oaf4lVT_Z7EHN1Gd.LqruAnRQB8znO7916E 4, 40 -- rVzWGffOARVcOTibJz2fNiU4lo3n0PdtpFcE0q.vloJZcbadxRw4Yz.Imabf 4, 40 -- xRFpEHrAdVXe2I7dF2.pUpc3u1iNy4Lg62DyVQB.eTg3ZBi20cDs4313_SKQ 4, 40 -- f9BtGF0xQWntzkOtRdh6tnlI3WHCCr2BVOJrLVggl0HYgZI3MS3lmqryLs_J 4, 40 -- W6hVbaadXW2IREayWJaexGrzv2dyh_FeAZ.ZJSQ2qydWE7vnut9Qjy.Z2mk9 4, 40 -- wnHjJRxbimkWdrb1u56FmYWMWpXnW2esLU89sbr9Lx.laC5.kfh6Z2YSuTJ9 4, 40 -- 7sYNjWQxutefjhRYyRSc7PIfKQ9nPSfMOOhzvXFqvnR2nvGHeDXFhL0LA.zl 4, 40 -- zwlMX1z2u6.A8OD_x1L0HaW1yCbJaHsL1YkpMvzNhSuxJm6_xC7TsZnRixg0 4, 40 -- A8v17s0wkKCo- 4, 40 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 40 -- Received: from [10.108.146.148] (vray-at-129.2.129.153 with plain) 4, 40 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 13 Oct 2011 13:44:56 -0700 PDT 4, 40 -- Message-ID: {4E974DC9.3000509-at-partbeamsystech.com} 4, 40 -- Date: Thu, 13 Oct 2011 16:44:57 -0400 4, 40 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 40 -- Reply-To: vray-at-partbeamsystech.com 4, 40 -- Organization: PBS&T 4, 40 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 4, 40 -- MIME-Version: 1.0 4, 40 -- To: jehrman-at-mta.ca 4, 40 -- CC: microscopy-at-microscopy.com 4, 40 -- Subject: Re: [Microscopy] vacuum gauge reading - resolved (?) 4, 40 -- References: {201110131610.p9DGAEPM020626-at-ns.microscopy.com} 4, 40 -- In-Reply-To: {201110131610.p9DGAEPM020626-at-ns.microscopy.com} 4, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 40 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
So, how do you all dispose of waste epoxy resin. We have been pouring it down the sink but they always get clogged and we have run out of drains that still work.
Not really. I'm good to go for higher concentrations of plastic, polymerize until solid then into the trash, but what about low conc. of plastic:solvent.
Like 1:3 plastic:acetone. That stuff never gets hard so I am looking for ideas about how to handle the waste.
Thanks
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 10, 34 -- From jkrupp-at-deltacollege.edu Thu Oct 13 16:52:54 2011 10, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 10, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9DLqspF027143 10, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 16:52:54 -0500 10, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 10, 34 -- by localhost (Email Security Appliance) with SMTP id 2809FA09A22_E975DB6B 10, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 21:52:54 +0000 (GMT) 10, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 10, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 0F680A09A58_E975DB6F 10, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 21:52:54 +0000 (GMT) 10, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 10, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 10, 34 -- ESMTP id LT0Y4500.4LZ for {Microscopy-at-microscopy.com} ; Thu, 13 10, 34 -- Oct 2011 14:52:53 -0700 10, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 10, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id D07AD12B345C4 10, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 14:52:53 -0700 (PDT) 10, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 10, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 10, 34 -- with ESMTP id sI-Bd2AnlkXH for {Microscopy-at-microscopy.com} ; 10, 34 -- Thu, 13 Oct 2011 14:52:50 -0700 (PDT) 10, 34 -- Received: from [172.20.3.99] (unknown [172.20.3.99]) 10, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 8BAE212B345AA 10, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 14:52:50 -0700 (PDT) 10, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 10, 34 -- Content-Type: text/plain; charset=us-ascii 10, 34 -- Subject: Resin disposal 10, 34 -- Date: Thu, 13 Oct 2011 14:52:50 -0700 10, 34 -- Message-Id: {3609099D-1D96-4EA0-BE6F-77851C56A67A-at-deltacollege.edu} 10, 34 -- To: Microscopy-at-microscopy.com 10, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 10, 34 -- X-Mailer: Apple Mail (2.1084) 10, 34 -- Content-Transfer-Encoding: 8bit 10, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DLqspF027143 ==============================End of - Headers==============================
I don't think there is a standard as to what file format to use or what image processing software to use, but there are ethical guidelines as to what can be done and what should not be done, and how the image manipulations should be documented. One such article you can find here:
The gist of both is that you need to stay as true as possible to the original image and pixel data (no compression), and that anything that could lead to artifacts needs to be documented, and, of course, that whatever you do to the image is documented in such a fashion that it can be repeated (as all scientific data should).
Mike Bode, Ph.D. ResAlta Research Technologies Corp.
2102 Beech Ct Golden, CO 80401 USA
p: +1-303-389-9839 f: +1-303-234-9270 Mike.Bode at ResAltaTech.com www.ResAltaTech.com
-----Original Message----- X-from: ZZhang-at-uwyo.edu [mailto:ZZhang-at-uwyo.edu] Sent: Thursday, October 13, 2011 5:36 PM To: mike.bode-at-resaltatech.com
Does anyone know if there is a "standard" for digital imaging, especially for microscopy related digital imaging?
If so, who is in charge of making such a standard?
Thank you,
Zhaojie Zhang, Ph. D. Director, Jenkins Microscopy Facility University of Wyoming Laramie, WY 82071 PHONE: 307-766-3038 FAX: 307-766-5625
==============================Original Headers============================== 11, 31 -- From ZZhang-at-uwyo.edu Thu Oct 13 18:28:42 2011 11, 31 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DNSfu2012687 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 18:28:41 -0500 11, 31 -- Received: from ponyexpress-ht3.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) 11, 31 -- by willowsprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9DNSbR7029726 11, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); 11, 31 -- Thu, 13 Oct 2011 17:28:37 -0600 (MDT) 11, 31 -- (envelope-from ZZhang-at-uwyo.edu) 11, 31 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by 11, 31 -- ponyexpress-ht3.uwyo.edu ([fe80::dc63:d646:8a62:d32f%13]) with mapi id 11, 31 -- 14.01.0289.001; Thu, 13 Oct 2011 17:28:37 -0600 11, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 11, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} , 11, 31 -- "CONFOCALMICROSCOPY-at-LISTS.UMN.EDU" {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} 11, 31 -- Subject: digital imaging standards 11, 31 -- Thread-Topic: digital imaging standards 11, 31 -- Thread-Index: AQHMif/VsONGQEb1nki2DYxCNhzWww== 11, 31 -- Date: Thu, 13 Oct 2011 23:28:37 +0000 11, 31 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D41AFBB-at-ponyexpress-m7.uwyo.edu} 11, 31 -- References: {201110132156.p9DLu73T032646-at-ns.microscopy.com} 11, 31 -- In-Reply-To: {201110132156.p9DLu73T032646-at-ns.microscopy.com} 11, 31 -- Accept-Language: en-US 11, 31 -- Content-Language: en-US 11, 31 -- X-MS-Has-Attach: 11, 31 -- X-MS-TNEF-Correlator: 11, 31 -- x-originating-ip: [10.56.56.50] 11, 31 -- Content-Type: text/plain; charset="us-ascii" 11, 31 -- MIME-Version: 1.0 11, 31 -- Content-Transfer-Encoding: 8bit 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DNSfu2012687 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 30 -- From mike.bode-at-resaltatech.com Thu Oct 13 19:04:43 2011 30, 30 -- Received: from resaltatech.com (resaltatech.com [99.198.119.35]) 30, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9E04gLo029519 30, 30 -- for {Microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 19:04:42 -0500 30, 30 -- Received: from c-98-245-223-115.hsd1.co.comcast.net ([98.245.223.115]:17153 helo=resdtpden01) 30, 30 -- by web38.justhost.com with esmtpa (Exim 4.69) 30, 30 -- (envelope-from {mike.bode-at-resaltatech.com} ) 30, 30 -- id 1REVGb-0002LT-Dt; Thu, 13 Oct 2011 19:04:49 -0500 30, 30 -- From: "Mike Bode" {mike.bode-at-resaltatech.com} 30, 30 -- To: {Microscopy-at-microscopy.com} 30, 30 -- References: {201110132336.p9DNaBWC025927-at-ns.microscopy.com} 30, 30 -- In-Reply-To: {201110132336.p9DNaBWC025927-at-ns.microscopy.com} 30, 30 -- Subject: RE: [Microscopy] digital imaging standards 30, 30 -- Date: Thu, 13 Oct 2011 18:04:33 -0600 30, 30 -- Message-ID: {007101cc8a04$dc244ad0$946ce070$-at-bode-at-resaltatech.com} 30, 30 -- MIME-Version: 1.0 30, 30 -- Content-Type: text/plain; 30, 30 -- charset="us-ascii" 30, 30 -- Content-Transfer-Encoding: 7bit 30, 30 -- X-Mailer: Microsoft Office Outlook 12.0 30, 30 -- Content-language: en-us 30, 30 -- Thread-index: AcyKAOmPp+4yJPPoQPetdpsR6zzaXwAAt89w 30, 30 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 30, 30 -- X-AntiAbuse: Primary Hostname - web38.justhost.com 30, 30 -- X-AntiAbuse: Original Domain - microscopy.com 30, 30 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 30, 30 -- X-AntiAbuse: Sender Address Domain - resaltatech.com 30, 30 -- X-Source: 30, 30 -- X-Source-Args: 30, 30 -- X-Source-Dir: ==============================End of - Headers==============================
I will be happy to collect the wagered money. I believe the bet was for the usual virtual "two cents". ;-)
Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
} From: {vray-at-partbeamsystech.com} } Reply-To: {vray-at-partbeamsystech.com} } Date: Thu, 13 Oct 2011 15:49:21 -0500 } To: {raristau-at-ims.uconn.edu} } Subject: [Microscopy] Re: vacuum gauge reading - resolved (?) } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Boo-hoooooo, I lost the money put on 2x10-5(( } } ;=))))) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 10/13/2011 12:10 PM, jehrman-at-mta.ca wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Denton's response: } } } } James } } The gauge is reading 2X10-6 I hope this helps } } } } Thank You } } George Mehaffey } } Field Service Manager } } Main # 856-439-9100 } } Desk # 856-380-5210 } } Cell # 609-304-9831 } } } } My inborn gauge reading skills are gloating, my intuition regarding vacuum } } systems is depressed. Now I must either have a stunningly high performance } } vacuum system, or a significantly miscalibrated gauge. Whoopee! } } } } Next time I'll try to post a less contentious question. For example: my } } Denton CPD is the only commercial CPD I'm aware of that doesn't have a } } viewing window. Good or bad thing? :-) } } } } As usual, thanks to all who took the time to post a reply. I apologize for } } any increases in blood pressure as a result. } } } } Jim } } } } } } ==============================Original Headers============================== } 4, 40 -- From vray-at-partbeamsystech.com Thu Oct 13 15:44:58 2011 } 4, 40 -- Received: from nm24.bullet.mail.sp2.yahoo.com } (nm24.bullet.mail.sp2.yahoo.com [98.139.91.94]) } 4, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id } p9DKivqj009371 } 4, 40 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 15:44:58 -0500 } 4, 40 -- Received: from [98.139.91.68] by nm24.bullet.mail.sp2.yahoo.com with } NNFMP; 13 Oct 2011 20:44:57 -0000 } 4, 40 -- Received: from [98.139.91.4] by tm8.bullet.mail.sp2.yahoo.com with } NNFMP; 13 Oct 2011 20:44:57 -0000 } 4, 40 -- Received: from [127.0.0.1] by omp1004.mail.sp2.yahoo.com with NNFMP; } 13 Oct 2011 20:44:57 -0000 } 4, 40 -- X-Yahoo-Newman-Id: 369482.5283.bm-at-omp1004.mail.sp2.yahoo.com } 4, 40 -- Received: (qmail 5346 invoked from network); 13 Oct 2011 20:44:56 } -0000 } 4, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; } s=s1024; t=1318538696; bh=FD0ulqennunVzjisbl5K1ZGe0sJvJf7e6VZAUWZsC7U=; } h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:Fr } om:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-R } eply-To:Content-Type:Content-Transfer-Encoding; } b=puao4d/xoE9ncgrTYGc6pGEcMoMwzWHRJdzEsfQHSTED3iNdjeiY3D5T8sTx2e1lTBtPvyb/Ynsq } rSD5y2s7KsnPr0rozdq4yhtyPoUtUk7HjW/yPNr3Y6c5y3kunLV6T/FzD7CJBZSJFOH+vKlpVXScfj } eHXaFKRoEKOpZyWYs= } 4, 40 -- X-Yahoo-Newman-Property: ymail-3 } 4, 40 -- X-YMail-OSG: bbfR2ykVM1lYLKX73AlwjHuGhNHy_ZQY9kUepKCL0udtpbs } 4, 40 -- KWIQ8qjS9sCq8XA7zOanJUa0CbvG.ZuqELZDOxZ9oRI1UYZEelOWTT4_caHP } 4, 40 -- 0EjurhIHov.Fd08Km5t0eFx9YKX5oR1L5vIu6yAw8.gzuAxKO1_JGIVw0ZKG } 4, 40 -- 8F9qvCnj7pcj7ddlDAeukXS71oaf4lVT_Z7EHN1Gd.LqruAnRQB8znO7916E } 4, 40 -- rVzWGffOARVcOTibJz2fNiU4lo3n0PdtpFcE0q.vloJZcbadxRw4Yz.Imabf } 4, 40 -- xRFpEHrAdVXe2I7dF2.pUpc3u1iNy4Lg62DyVQB.eTg3ZBi20cDs4313_SKQ } 4, 40 -- f9BtGF0xQWntzkOtRdh6tnlI3WHCCr2BVOJrLVggl0HYgZI3MS3lmqryLs_J } 4, 40 -- W6hVbaadXW2IREayWJaexGrzv2dyh_FeAZ.ZJSQ2qydWE7vnut9Qjy.Z2mk9 } 4, 40 -- wnHjJRxbimkWdrb1u56FmYWMWpXnW2esLU89sbr9Lx.laC5.kfh6Z2YSuTJ9 } 4, 40 -- 7sYNjWQxutefjhRYyRSc7PIfKQ9nPSfMOOhzvXFqvnR2nvGHeDXFhL0LA.zl } 4, 40 -- zwlMX1z2u6.A8OD_x1L0HaW1yCbJaHsL1YkpMvzNhSuxJm6_xC7TsZnRixg0 } 4, 40 -- A8v17s0wkKCo- } 4, 40 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- } 4, 40 -- Received: from [10.108.146.148] (vray-at-129.2.129.153 with plain) } 4, 40 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 13 Oct 2011 } 13:44:56 -0700 PDT } 4, 40 -- Message-ID: {4E974DC9.3000509-at-partbeamsystech.com} } 4, 40 -- Date: Thu, 13 Oct 2011 16:44:57 -0400 } 4, 40 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} } 4, 40 -- Reply-To: vray-at-partbeamsystech.com } 4, 40 -- Organization: PBS&T } 4, 40 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 } Thunderbird/7.0.1 } 4, 40 -- MIME-Version: 1.0 } 4, 40 -- To: jehrman-at-mta.ca } 4, 40 -- CC: microscopy-at-microscopy.com } 4, 40 -- Subject: Re: [Microscopy] vacuum gauge reading - resolved (?) } 4, 40 -- References: {201110131610.p9DGAEPM020626-at-ns.microscopy.com} } 4, 40 -- In-Reply-To: {201110131610.p9DGAEPM020626-at-ns.microscopy.com} } 4, 40 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 4, 40 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 6, 23 -- From raristau-at-ims.uconn.edu Fri Oct 14 08:45:07 2011 6, 23 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 6, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9EDj7nV025568 6, 23 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 08:45:07 -0500 6, 23 -- Received: from [137.99.20.184] (d20h184.public.uconn.edu [137.99.20.184]) 6, 23 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id CC9E734B7E; 6, 23 -- Fri, 14 Oct 2011 09:45:06 -0400 (EDT) 6, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122 6, 23 -- Date: Fri, 14 Oct 2011 09:45:04 -0400 6, 23 -- Subject: Re: [Microscopy] Re: vacuum gauge reading - resolved (?) 6, 23 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 6, 23 -- To: {vray-at-partbeamsystech.com} , 6, 23 -- "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} 6, 23 -- Message-ID: {CABDB520.3262%raristau-at-ims.uconn.edu} 6, 23 -- Thread-Topic: [Microscopy] Re: vacuum gauge reading - resolved (?) 6, 23 -- Thread-Index: AcyKd3oIuGiTgvZqEeCTAQAbY55CVA== 6, 23 -- In-Reply-To: {201110132049.p9DKnLEQ015022-at-ns.microscopy.com} 6, 23 -- Mime-version: 1.0 6, 23 -- Content-type: text/plain; 6, 23 -- charset="US-ASCII" 6, 23 -- Content-transfer-encoding: 7bit 6, 23 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 6, 23 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Do not use a "lossy" compression, ie. JPEG. Use TIFF to save your images. Journals will publish guidelines in the "Instructions to Authors" section as to what, if any, modifications may be allowed. Always save the original, unaltered image file and a backup.
Geoff
On 10/13/2011 7:29 PM, ZZhang-at-uwyo.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Does anyone know if there is a "standard" for digital imaging, especially for microscopy related digital imaging? } } If so, who is in charge of making such a standard? } } Thank you, } } } Zhaojie Zhang, Ph. D. } Director, Jenkins Microscopy Facility } University of Wyoming } Laramie, WY 82071 } PHONE: 307-766-3038 } FAX: 307-766-5625 } } } } } } } } ==============================Original Headers============================== } 11, 31 -- From ZZhang-at-uwyo.edu Thu Oct 13 18:28:42 2011 } 11, 31 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) } 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DNSfu2012687 } 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 18:28:41 -0500 } 11, 31 -- Received: from ponyexpress-ht3.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) } 11, 31 -- by willowsprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9DNSbR7029726 } 11, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); } 11, 31 -- Thu, 13 Oct 2011 17:28:37 -0600 (MDT) } 11, 31 -- (envelope-from ZZhang-at-uwyo.edu) } 11, 31 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by } 11, 31 -- ponyexpress-ht3.uwyo.edu ([fe80::dc63:d646:8a62:d32f%13]) with mapi id } 11, 31 -- 14.01.0289.001; Thu, 13 Oct 2011 17:28:37 -0600 } 11, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } 11, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} , } 11, 31 -- "CONFOCALMICROSCOPY-at-LISTS.UMN.EDU" {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} } 11, 31 -- Subject: digital imaging standards } 11, 31 -- Thread-Topic: digital imaging standards } 11, 31 -- Thread-Index: AQHMif/VsONGQEb1nki2DYxCNhzWww== } 11, 31 -- Date: Thu, 13 Oct 2011 23:28:37 +0000 } 11, 31 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D41AFBB-at-ponyexpress-m7.uwyo.edu} } 11, 31 -- References: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } 11, 31 -- In-Reply-To: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } 11, 31 -- Accept-Language: en-US } 11, 31 -- Content-Language: en-US } 11, 31 -- X-MS-Has-Attach: } 11, 31 -- X-MS-TNEF-Correlator: } 11, 31 -- x-originating-ip: [10.56.56.50] } 11, 31 -- Content-Type: text/plain; charset="us-ascii" } 11, 31 -- MIME-Version: 1.0 } 11, 31 -- Content-Transfer-Encoding: 8bit } 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DNSfu2012687 } ==============================End of - Headers============================== } }
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff-at-umdnj.edu **********************************************
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both keith.daum-at-stryker.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: I need a copy of the installation manual for a Philips XL30 SEM. It is a 1993 vintage SEM with a manual stage. Either a hard copy or soft copy would be greatly appreciated.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both aap-at-ust.hk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: aap-at-ust.hk Name: Aslan
Organization: Hong Kong University of Science and Technology
Message: I am working on NiTi (B2 phase) shape memory alloys. I think all of the planes diffract and I do not know how to index my ring pattern. Can any body help?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kcildavis02-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: kcildavis02-at-gmail.com Name: Christie Davis
Title-Subject: [Filtered] Does HF effect contrast?
Message: Good morning. I am using HF (hydrofluoric acid) to etch away spicules(silicon) from epon embedded sponge tissue. This MAY be decreasing the contrast of the tissue when sectioned and viewed in the TEM. Has anyone had this experience? Thank you,
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both smithran-at-pdx.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: smithran-at-pdx.edu Name: RANDALL SMITH
Organization: PRESIDENT, PACIFIC NORTHWEST MICROSCOPY SOCIETY (PNMS)
Message: October 14th, 2011, Friday, Portland, Oregon
Dear Members and Friends,
Emertia Professor of Physics, Portland State University, Gertrude F. Rempfer, passed away October 4th after a brief infection. She was 99.
She was honored as an MSA FELLOW IN 2009, receiving the honor at M&M2010 in Portland, Oregon, where there was a standing ovation at the presidential openings that day. It was a well deserved honor so someone active in microscopy for such a long period of time. She is, indeed part of our history as microscopists. Gert received BS and PhD degrees from the University of Washington in 1934 and 1939 respectively. She held several teaching posts, but during WWII she worked for the Naval Research laboratory, Washington D.C. 1942-43, and at the S.A.M. Laboratory, Columbia University (Manhattan Project) in 1944. She and her husband, Robert Rempfer, came to Portland State University in 1959, he in Mathematics, she in Physics. She was an early member of the Pacific Northwest Microscopy Society in instrumental in collegial and cooperative work in instrument operation. She gave full meaning to the operation of the local society for collegial and cooperative benefit of all interested electron microscopists. PNMS cherishes her participation and active membership over these many years.
Gert received several honors and awards, the Vollum Award for Distinguished Accomplishment in Science and Technology, in 1987 the EMSA Distinguished Scientist award in physics sciences in 1990, the Oregon Academy of Science, Outstanding Scientist of the Year in 1998, and many others.
Gert was an outstanding teacher as well as researcher. She gave freely of her technical expertise in electrostatic lens design and photoelectron microscopy. She was instrumental in the development of a microscope with electrostatic lenses, the Elektros. Her research yielded the world's first untra-high vacuum photoemission electron microscope in 1978. She developed two key elements of developing the achromat electron lens by electron mirror and beam separation system. She continued an active research life beyond official retirement, continuing these many projects with several graduate students. Gert gave freely to everyone who entered her laboratory. We have lost nothing in her passing. We have everything she was able to give to us - they were her gifts to us to use wisely, and we who knew her have every memory of her excellent work. What we do not have is her mind working with us on our future projects, and it was a unique and cherished mind, indeed. A Remarkable Woman In Science.
Randall W. Smith, President Pacific Northwest Microscopy Society (PNMS) and Dept of Physics Portland State University P.O. Box 751-PHY, Portland, Oregon 97207
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rbrown-at-mvainc.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: rbrown-at-mvainc.com Name: Rich brown
Organization: MVA Scientific Consultants
Title-Subject: [Filtered] Digital imaging
Message: Answers to many questions can be found here http://www.theiai.org/guidelines/swgit/ Most of the definitions and procedures concern legal requirements and are applicable to good laboratory practices
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both dcechet-at-ctarg.com.ar as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: dcechet-at-ctarg.com.ar Name: Daniel
Title-Subject: [Filtered] TEM Screen Recoating
Message: Hi
We have an old Zeiss EM 10 and we have to recoat the screen viewing. Does anyone know how to do this?
Hi, have a look at http://www.laddresearch.com/General_Catalog/Chapter_3/Fluorescent_Screens/fluorescent_screens.html ...Just a satisfied customer.... I think their pricing is very reasonable. Just send an old screen over.
In my opinion you will spend too much money to make the screen on your own (with many trials and problems to solve; and I know that because I did it myself ten years ago ;-) )
Best wishes, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 15.10.11 16:13, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both dcechet-at-ctarg.com.ar as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: dcechet-at-ctarg.com.ar Name: Daniel } } Title-Subject: [Filtered] TEM Screen Recoating } } Message: Hi } } We have an old Zeiss EM 10 and we have to recoat the screen viewing. } Does anyone know how to do this? } } Thanks in advance } } Daniel } } } } Login Host: 190.226.190.127 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Oct 15 09:09:22 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FE9MI9001930 } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:22 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 52DB64288BC } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:22 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id vhLfze7NQZos for {microscopy-at-microscopy.com} ; } 12, 25 -- Sat, 15 Oct 2011 09:09:18 -0500 (CDT) } 12, 25 -- Received: from NestorPwrBk.local (unknown [187.126.150.126]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id B162C4288AC } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:17 -0500 (CDT) } 12, 25 -- Message-ID: {4E99940A.7070504-at-microscopy.com} } 12, 25 -- Date: Sat, 15 Oct 2011 09:09:14 -0500 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: dcechet-at-ctarg.com.ar } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW:TEM Screen Recoating } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 11, 22 -- From stefan.diller-at-t-online.de Sat Oct 15 09:39:25 2011 11, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FEdPro019618 11, 22 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:39:25 -0500 11, 22 -- Received: from fwd14.aul.t-online.de (fwd14.aul.t-online.de ) 11, 22 -- by mailout08.t-online.de with smtp 11, 22 -- id 1RF5OW-0003Ym-8n; Sat, 15 Oct 2011 16:39:24 +0200 11, 22 -- Received: from mac-pro.speedport.ip (JOwn3iZ1QhFfTKOMKMJcUbDbQW2BZ16hs-o3M77rscIG5L4czPBt-N3ZiYuATRtwrz-at-[93.222.120.169]) by fwd14.t-online.de 11, 22 -- with esmtp id 1RF5OV-0EPgwq0; Sat, 15 Oct 2011 16:39:23 +0200 11, 22 -- Message-ID: {4E999B1A.3030709-at-t-online.de} 11, 22 -- Date: Sat, 15 Oct 2011 16:39:22 +0200 11, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 11, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 11, 22 -- MIME-Version: 1.0 11, 22 -- To: microscopy-at-microscopy.com 11, 22 -- Subject: Re: [Microscopy] viaWWW:TEM Screen Recoating 11, 22 -- References: {201110151413.p9FEDNZ9008055-at-ns.microscopy.com} 11, 22 -- In-Reply-To: {201110151413.p9FEDNZ9008055-at-ns.microscopy.com} 11, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 22 -- Content-Transfer-Encoding: 7bit 11, 22 -- X-ID: JOwn3iZ1QhFfTKOMKMJcUbDbQW2BZ16hs-o3M77rscIG5L4czPBt-N3ZiYuATRtwrz 11, 22 -- X-TOI-MSGID: 8c0cadee-a982-4015-8ced-ea4346f99422 ==============================End of - Headers==============================
We've had good luck with Grant Scientific (http://www.mindspring.com/~grantscientific, Dana Dunkelberger).
No financial interest, just a satisfied customer.
Cheers, Henk
At 10/15/2011 10:10 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both dcechet-at-ctarg.com.ar as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: dcechet-at-ctarg.com.ar Name: Daniel } } Title-Subject: [Filtered] TEM Screen Recoating } } Message: Hi } } We have an old Zeiss EM 10 and we have to recoat the screen viewing. } Does anyone know how to do this? } } Thanks in advance } } Daniel } } } } Login Host: 190.226.190.127 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Oct 15 09:09:22 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FE9MI9001930 } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:22 -0500 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 52DB64288BC } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:22 -0500 (CDT) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id vhLfze7NQZos for {microscopy-at-microscopy.com} ; } 12, 25 -- Sat, 15 Oct 2011 09:09:18 -0500 (CDT) } 12, 25 -- Received: from NestorPwrBk.local (unknown [187.126.150.126]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id B162C4288AC } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:17 -0500 (CDT) } 12, 25 -- Message-ID: {4E99940A.7070504-at-microscopy.com} } 12, 25 -- Date: Sat, 15 Oct 2011 09:09:14 -0500 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: dcechet-at-ctarg.com.ar } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW:TEM Screen Recoating } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 8, 27 -- From colijn.1-at-osu.edu Sat Oct 15 10:47:46 2011 8, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FFljoA006728 8, 27 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 10:47:46 -0500 8, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 8, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 27 -- id {01O78U6D7NVK93SC4O-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 8, 27 -- Sat, 15 Oct 2011 11:47:45 -0400 (EDT) 8, 27 -- Received: from [192.168.1.103] 8, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 8, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 8, 27 -- with ESMTPA id {01O78U680LDM93SRV7-at-ecr6.ohio-state.edu} ; Sat, 8, 27 -- 15 Oct 2011 11:47:44 -0400 (EDT) 8, 27 -- Date: Sat, 15 Oct 2011 11:47:37 -0400 8, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 8, 27 -- Subject: Re: [Microscopy] viaWWW:TEM Screen Recoating 8, 27 -- In-reply-to: {201110151410.p9FEAXKA003838-at-ns.microscopy.com} 8, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 8, 27 -- To: microscopy-at-microscopy.com, dcechet-at-ctarg.com.ar 8, 27 -- Message-id: {4E99AB19.7020207-at-osu.edu} 8, 27 -- MIME-version: 1.0 8, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 8, 27 -- Content-transfer-encoding: 7bit 8, 27 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 8, 27 -- Thunderbird/7.0.1 8, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 8, 27 -- References: {201110151410.p9FEAXKA003838-at-ns.microscopy.com} ==============================End of - Headers==============================
I'll second Henk's comment. We've always had good service from Dana. JEOL use him too, or at least they used to. No financial interest. Just a satisfied customer.
Sent from my iPad. -- John Mansfield PhD Cphys MInstP 2010 Microscopy & Microanalysis Program Chair North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913
4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
On Oct 15, 2011, at 11:55 AM, colijn.1-at-osu.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Daniel, } } We've had good luck with Grant Scientific } (http://www.mindspring.com/~grantscientific, Dana Dunkelberger). } } No financial interest, just a satisfied customer. } } Cheers, } Henk } } } At 10/15/2011 10:10 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both dcechet-at-ctarg.com.ar as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: dcechet-at-ctarg.com.ar Name: Daniel } } } } Title-Subject: [Filtered] TEM Screen Recoating } } } } Message: Hi } } } } We have an old Zeiss EM 10 and we have to recoat the screen viewing. } } Does anyone know how to do this? } } } } Thanks in advance } } } } Daniel } } } } } } } } Login Host: 190.226.190.127 } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Oct 15 09:09:22 2011 } } 12, 25 -- Received: from znl.com ([206.69.208.20]) } } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FE9MI9001930 } } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:22 -0500 } } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } } 12, 25 -- by znl.com (Postfix) with ESMTP id 52DB64288BC } } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:22 -0500 (CDT) } } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 12, 25 -- Received: from znl.com ([127.0.0.1]) } } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } } 12, 25 -- with ESMTP id vhLfze7NQZos for {microscopy-at-microscopy.com} ; } } 12, 25 -- Sat, 15 Oct 2011 09:09:18 -0500 (CDT) } } 12, 25 -- Received: from NestorPwrBk.local (unknown [187.126.150.126]) } } 12, 25 -- by znl.com (Postfix) with ESMTPA id B162C4288AC } } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 09:09:17 -0500 (CDT) } } 12, 25 -- Message-ID: {4E99940A.7070504-at-microscopy.com} } } 12, 25 -- Date: Sat, 15 Oct 2011 09:09:14 -0500 } } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } } 12, 25 -- Reply-To: dcechet-at-ctarg.com.ar } } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 12, 25 -- MIME-Version: 1.0 } } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 12, 25 -- Subject: viaWWW:TEM Screen Recoating } } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 12, 25 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } -- } Hendrik O. Colijn } www.ceof.ohio-state.edu } OSU Campus Electron Optics Facility colijn.1-at-osu.edu } 040 Fontana Labs (614) 292-0674 (V) } 116 W. 19th Ave. (614) 292-7523 (F) } Columbus, OH 43210 } } "Time is that quality of nature which keeps things from happening all at } once. Lately it doesn't seem to be working." } } ==============================Original Headers============================== } 8, 27 -- From colijn.1-at-osu.edu Sat Oct 15 10:47:46 2011 } 8, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) } 8, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FFljoA006728 } 8, 27 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 10:47:46 -0500 } 8, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by } 8, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) } 8, 27 -- id {01O78U6D7NVK93SC4O-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; } 8, 27 -- Sat, 15 Oct 2011 11:47:45 -0400 (EDT) } 8, 27 -- Received: from [192.168.1.103] } 8, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) } 8, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) } 8, 27 -- with ESMTPA id {01O78U680LDM93SRV7-at-ecr6.ohio-state.edu} ; Sat, } 8, 27 -- 15 Oct 2011 11:47:44 -0400 (EDT) } 8, 27 -- Date: Sat, 15 Oct 2011 11:47:37 -0400 } 8, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} } 8, 27 -- Subject: Re: [Microscopy] viaWWW:TEM Screen Recoating } 8, 27 -- In-reply-to: {201110151410.p9FEAXKA003838-at-ns.microscopy.com} } 8, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu } 8, 27 -- To: microscopy-at-microscopy.com, dcechet-at-ctarg.com.ar } 8, 27 -- Message-id: {4E99AB19.7020207-at-osu.edu} } 8, 27 -- MIME-version: 1.0 } 8, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed } 8, 27 -- Content-transfer-encoding: 7bit } 8, 27 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 } 8, 27 -- Thunderbird/7.0.1 } 8, 27 -- X-Env-From: auth/colijn.1-at-osu.edu } 8, 27 -- References: {201110151410.p9FEAXKA003838-at-ns.microscopy.com} } ==============================End of - Headers============================== } }
==============================Original Headers============================== 7, 21 -- From jfmjfm-at-umich.edu Sat Oct 15 13:24:08 2011 7, 21 -- Received: from hellskitchen.mr.itd.umich.edu (smtp.mail.umich.edu [141.211.14.82]) 7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9FIO8uG025367 7, 21 -- for {microscopy-at-microscopy.com} ; Sat, 15 Oct 2011 13:24:08 -0500 7, 21 -- Received: FROM [10.0.1.174] (c-68-40-166-190.hsd1.mi.comcast.net [68.40.166.190]) 7, 21 -- By hellskitchen.mr.itd.umich.edu ID 4E99CFC5.6B6FB.27254 ; 7, 21 -- Authuser jfmjfm; 7, 21 -- 15 Oct 2011 14:24:05 EDT 7, 21 -- References: {201110151555.p9FFthDM020344-at-ns.microscopy.com} 7, 21 -- In-Reply-To: {201110151555.p9FFthDM020344-at-ns.microscopy.com} 7, 21 -- Mime-Version: 1.0 (1.0) 7, 21 -- Content-Type: text/plain; 7, 21 -- charset=utf-8 7, 21 -- Message-Id: {20A8DD23-A9DE-4266-BCEB-FEC5849428CA-at-umich.edu} 7, 21 -- X-Mailer: iPad Mail (9A334) 7, 21 -- From: John Mansfield {jfmjfm-at-umich.edu} 7, 21 -- Subject: Re: [Microscopy] Re: viaWWW:TEM Screen Recoating 7, 21 -- Date: Sat, 15 Oct 2011 14:24:02 -0400 7, 21 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 21 -- Content-Transfer-Encoding: 8bit 7, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9FIO8uG025367 ==============================End of - Headers==============================
From isabecaromell201102-at-yahoo.com.hk Mon Oct 17 04:50:36 2011 Return-Path: {isabecaromell201102-at-yahoo.com.hk} Received: from mail.ldshop.com.tw (220-134-38-11.HINET-IP.hinet.net [220.134.38.11]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9H9oZE8005415; Mon, 17 Oct 2011 04:50:36 -0500 Received: from User (unknown [46.183.217.253]) by mail.ldshop.com.tw (Postfix) with ESMTP id 794843A6F64; Mon, 17 Oct 2011 15:00:13 +0800 (CST) Reply-To: {cmrs.isabella-at-yahoo.com.hk}
I beg to differ-JPEG is nearly indistinguishable from TIFF for photographic reproduction. That is one reason why all of the digital cameras in the world default to JPEG, even the expensive digital SLR's. The only place in a micrograph where you can actually discern compression artifacts is around micron markers or other text, and even that is only visible by zooming in on the region. BTW, as soon as you insert that image into PowerPoint for a report or presentation, it is automatically compressed anyway. However, I do agree that one should archive the raw original DM or whatever format the camera produces.
John Mardinly ASU
} } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Do not use a "lossy" compression, ie. JPEG. Use TIFF to save your } images. Journals will publish guidelines in the "Instructions to } Authors" section as to what, if any, modifications may be allowed. } Always save the original, unaltered image file and a backup. } } Geoff } } On 10/13/2011 7:29 PM, ZZhang-at-uwyo.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Does anyone know if there is a "standard" for digital imaging, especially for microscopy related digital imaging? } } } } If so, who is in charge of making such a standard? } } } } Thank you, } } } } } } Zhaojie Zhang, Ph. D. } } Director, Jenkins Microscopy Facility } } University of Wyoming } } Laramie, WY 82071 } } PHONE: 307-766-3038 } } FAX: 307-766-5625 } } } } } } } } } } } } } } } } ==============================Original Headers============================== } } 11, 31 -- From ZZhang-at-uwyo.edu Thu Oct 13 18:28:42 2011 } } 11, 31 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) } } 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DNSfu2012687 } } 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 18:28:41 -0500 } } 11, 31 -- Received: from ponyexpress-ht3.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) } } 11, 31 -- by willowsprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9DNSbR7029726 } } 11, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); } } 11, 31 -- Thu, 13 Oct 2011 17:28:37 -0600 (MDT) } } 11, 31 -- (envelope-from ZZhang-at-uwyo.edu) } } 11, 31 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by } } 11, 31 -- ponyexpress-ht3.uwyo.edu ([fe80::dc63:d646:8a62:d32f%13]) with mapi id } } 11, 31 -- 14.01.0289.001; Thu, 13 Oct 2011 17:28:37 -0600 } } 11, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } } 11, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} , } } 11, 31 -- "CONFOCALMICROSCOPY-at-LISTS.UMN.EDU" {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} } } 11, 31 -- Subject: digital imaging standards } } 11, 31 -- Thread-Topic: digital imaging standards } } 11, 31 -- Thread-Index: AQHMif/VsONGQEb1nki2DYxCNhzWww== } } 11, 31 -- Date: Thu, 13 Oct 2011 23:28:37 +0000 } } 11, 31 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D41AFBB-at-ponyexpress-m7.uwyo.edu} } } 11, 31 -- References: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } 11, 31 -- In-Reply-To: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } 11, 31 -- Accept-Language: en-US } } 11, 31 -- Content-Language: en-US } } 11, 31 -- X-MS-Has-Attach: } } 11, 31 -- X-MS-TNEF-Correlator: } } 11, 31 -- x-originating-ip: [10.56.56.50] } } 11, 31 -- Content-Type: text/plain; charset="us-ascii" } } 11, 31 -- MIME-Version: 1.0 } } 11, 31 -- Content-Transfer-Encoding: 8bit } } 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DNSfu2012687 } } ==============================End of - Headers============================== } } } } } } } -- } -- } ********************************************** } Geoff McAuliffe, Ph.D. } Neuroscience and Cell Biology } Robert Wood Johnson Medical School } 675 Hoes Lane, Piscataway, NJ 08854 } voice: (732)-235-4583 } mcauliff-at-umdnj.edu } ********************************************** } } } } ==============================Original Headers============================== } 7, 62 -- From mcauliff-at-umdnj.edu Fri Oct 14 12:27:03 2011 } 7, 62 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) } 7, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9EHR3cc001954 } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 12:27:03 -0500 } 7, 62 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) } 7, 62 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E27A221CF927 } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } 7, 62 -- Received: from umdnj.edu (unknown [10.32.15.102]) } 7, 62 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 661E621CF913 } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } 7, 62 -- Received: from ([10.32.15.102]) } 7, 62 -- by imail.umdnj.edu with ESMTP with TLS id 8XSJWG1.87825724; } 7, 62 -- Fri, 14 Oct 2011 13:26:58 -0400 } 7, 62 -- MIME-version: 1.0 } 7, 62 -- Content-transfer-encoding: 7BIT } 7, 62 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed } 7, 62 -- Received: from [127.0.0.1] ([10.32.15.102]) } 7, 62 -- by scpmmp2.umdnj.edu (Oracle Communications Messaging Exchange Server } 7, 62 -- 7u4-22.01 64bit (built Apr 21 2011)) } 7, 62 -- with ESMTP id {0LT200H2HGGXOFB0-at-scpmmp2.umdnj.edu} for } 7, 62 -- microscopy-at-microscopy.com; Fri, 14 Oct 2011 13:26:58 -0400 (EDT) } 7, 62 -- Message-id: {4E987115.1020809-at-umdnj.edu} } 7, 62 -- Date: Fri, 14 Oct 2011 13:27:49 -0400 } 7, 62 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} } 7, 62 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.22) } 7, 62 -- Gecko/20110902 Thunderbird/3.1.14 } 7, 62 -- To: ZZhang-at-uwyo.edu, microscopy-at-microscopy.com } 7, 62 -- Subject: Re: [Microscopy] digital imaging standards } 7, 62 -- References: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } 7, 62 -- In-reply-to: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } 7, 62 -- X-esp: ESP {11} = } 7, 62 -- SHA: {0} } 7, 62 -- SHA_FLAGS: {0} } 7, 62 -- UHA: {10} } 7, 62 -- ISC: {0} } 7, 62 -- BAYES: {0} } 7, 62 -- SenderID: {0} } 7, 62 -- DKIM: {0} } 7, 62 -- TS: {0} } 7, 62 -- SIG: {} } 7, 62 -- DSC: {0} } 7, 62 -- TRU_freehosting: {0} } 7, 62 -- TRU_misc_spam: {0} } 7, 62 -- TRU_marketing_spam: {0} } 7, 62 -- TRU_profanity_spam: {0} } 7, 62 -- TRU_spam1: {0} } 7, 62 -- TRU_scam_spam: {0} } 7, 62 -- TRU_money_spam: {0} } 7, 62 -- URL Real-Time Signatures: {0} } 7, 62 -- TRU_watch_spam: {0} } 7, 62 -- TRU_embedded_image_spam: {1} } 7, 62 -- TRU_spam2: {0} } 7, 62 -- TRU_medical_spam: {0} } 7, 62 -- TRU_playsites: {0} } 7, 62 -- TRU_html_image_spam: {0} } 7, 62 -- TRU_stock_spam: {0} } 7, 62 -- TRU_urllinks: {0} } 7, 62 -- TRU_adult_spam: {0} } 7, 62 -- TRU_legal_spam: {0} } 7, 62 -- TRU_lotto_spam: {0} } 7, 62 -- TRU_ru_spamsubj: {0} } 7, 62 -- TRU_phish_spam: {0} } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 39 -- From John.Mardinly-at-asu.edu Mon Oct 17 12:32:40 2011 5, 39 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HHWe4A013994 5, 39 -- for {Microscopy-at-Microscopy.com} ; Mon, 17 Oct 2011 12:32:40 -0500 5, 39 -- X-ASG-Debug-ID: 1318872756-03efbe6e79fce090003-FOsErg 5, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id FSFmKdpGWqRjP8RZ (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Mon, 17 Oct 2011 10:32:37 -0700 (MST) 5, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu 5, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 5, 39 -- X-ASG-Whitelist: Client 5, 39 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by 5, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id 5, 39 -- 8.2.255.0; Mon, 17 Oct 2011 10:32:36 -0700 5, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by 5, 39 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Mon, 17 Oct 5, 39 -- 2011 10:32:32 -0700 5, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu 5, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Mon, 17 Oct 2011 5, 39 -- 17:32:32 +0000 5, 39 -- Subject: Re: [Microscopy] Re: digital imaging standards 5, 39 -- References: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} 5, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: digital imaging standards 5, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} 5, 39 -- Content-Type: text/plain; charset="us-ascii" 5, 39 -- In-Reply-To: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} 5, 39 -- Message-ID: {94BA1879-94B5-4C98-ADE4-0231231FCC76-at-exchange.asu.edu} 5, 39 -- Thread-Topic: [Microscopy] Re: digital imaging standards 5, 39 -- Thread-Index: AcyM8sBlFrqgOqJPS1KyooS3hOPGlQ== 5, 39 -- Date: Mon, 17 Oct 2011 10:37:53 -0700 5, 39 -- To: {mcauliff-at-umdnj.edu} , MSA {Microscopy-at-Microscopy.com} 5, 39 -- CC: {John.Mardinly-at-asu.edu} 5, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) 5, 39 -- X-OriginalArrivalTime: 17 Oct 2011 17:32:32.0980 (UTC) FILETIME=[C0B28140:01CC8CF2] 5, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] 5, 39 -- X-Barracuda-Start-Time: 1318872757 5, 39 -- X-Barracuda-Encrypted: RC4-MD5 5, 39 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi 5, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu 5, 39 -- Content-Transfer-Encoding: 8bit 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HHWe4A013994 ==============================End of - Headers==============================
By human eye you may not be able distinguish a JPEG from a TIFF.
However our images are scientific data, and we end up doing quantitative analysis on this data.
JPEG formating CHANGES the data. What is even worse if you take a JPEG image and store it a second time CHANGES are made to the last set of CHANGES. This corrupts the integrity of the scientific data.
Do a simple test. Take an image store it in TIFF. Then store it as JPEG, then open the JPEG and compare the results pixel by pixel. You no longer have the same information. If you then store the JPEG again the compression routine reformat the data yet again.
You should always, repeat always, store your original data in a lossless format. RAW, TIFF are two of the examples. You can just JPEG to post on the WWW or print posters, but never when any quantitative work is being done from that data set.
Nestor (steps down from his soap box) Your Friendly Neighborhood SysOp
On 10/17/11 3:32 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I beg to differ-JPEG is nearly indistinguishable from TIFF for photographic reproduction. That is one reason why all of the digital cameras in the world default to JPEG, even the expensive digital SLR's. The only place in a micrograph where you can actually discern compression artifacts is around micron markers or other text, and even that is only visible by zooming in on the region. BTW, as soon as you insert that image into PowerPoint for a report or presentation, it is automatically compressed anyway. However, I do agree that one should archive the raw original DM or whatever format the camera produces. } } John Mardinly } ASU } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Do not use a "lossy" compression, ie. JPEG. Use TIFF to save your } } images. Journals will publish guidelines in the "Instructions to } } Authors" section as to what, if any, modifications may be allowed. } } Always save the original, unaltered image file and a backup. } } } } Geoff } } } } On 10/13/2011 7:29 PM, ZZhang-at-uwyo.edu wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Does anyone know if there is a "standard" for digital imaging, especially for microscopy related digital imaging? } } } } } } If so, who is in charge of making such a standard? } } } } } } Thank you, } } } } } } } } } Zhaojie Zhang, Ph. D. } } } Director, Jenkins Microscopy Facility } } } University of Wyoming } } } Laramie, WY 82071 } } } PHONE: 307-766-3038 } } } FAX: 307-766-5625 } } } } } } } } } } } } } } } } } } } } } } } } ==============================Original Headers============================== } } } 11, 31 -- From ZZhang-at-uwyo.edu Thu Oct 13 18:28:42 2011 } } } 11, 31 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) } } } 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DNSfu2012687 } } } 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 18:28:41 -0500 } } } 11, 31 -- Received: from ponyexpress-ht3.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) } } } 11, 31 -- by willowsprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9DNSbR7029726 } } } 11, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); } } } 11, 31 -- Thu, 13 Oct 2011 17:28:37 -0600 (MDT) } } } 11, 31 -- (envelope-from ZZhang-at-uwyo.edu) } } } 11, 31 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by } } } 11, 31 -- ponyexpress-ht3.uwyo.edu ([fe80::dc63:d646:8a62:d32f%13]) with mapi id } } } 11, 31 -- 14.01.0289.001; Thu, 13 Oct 2011 17:28:37 -0600 } } } 11, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } } } 11, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} , } } } 11, 31 -- "CONFOCALMICROSCOPY-at-LISTS.UMN.EDU" {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} } } } 11, 31 -- Subject: [Filtered] digital imaging standards } } } 11, 31 -- Thread-Topic: digital imaging standards } } } 11, 31 -- Thread-Index: AQHMif/VsONGQEb1nki2DYxCNhzWww== } } } 11, 31 -- Date: Thu, 13 Oct 2011 23:28:37 +0000 } } } 11, 31 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D41AFBB-at-ponyexpress-m7.uwyo.edu} } } } 11, 31 -- References: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } } 11, 31 -- In-Reply-To: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } } 11, 31 -- Accept-Language: en-US } } } 11, 31 -- Content-Language: en-US } } } 11, 31 -- X-MS-Has-Attach: } } } 11, 31 -- X-MS-TNEF-Correlator: } } } 11, 31 -- x-originating-ip: [10.56.56.50] } } } 11, 31 -- Content-Type: text/plain; charset="us-ascii" } } } 11, 31 -- MIME-Version: 1.0 } } } 11, 31 -- Content-Transfer-Encoding: 8bit } } } 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DNSfu2012687 } } } ==============================End of - Headers============================== } } } } } } } } } } } } -- } } -- } } ********************************************** } } Geoff McAuliffe, Ph.D. } } Neuroscience and Cell Biology } } Robert Wood Johnson Medical School } } 675 Hoes Lane, Piscataway, NJ 08854 } } voice: (732)-235-4583 } } mcauliff-at-umdnj.edu } } ********************************************** } } } } } } } } ==============================Original Headers============================== } } 7, 62 -- From mcauliff-at-umdnj.edu Fri Oct 14 12:27:03 2011 } } 7, 62 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) } } 7, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9EHR3cc001954 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 12:27:03 -0500 } } 7, 62 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) } } 7, 62 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E27A221CF927 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } } 7, 62 -- Received: from umdnj.edu (unknown [10.32.15.102]) } } 7, 62 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 661E621CF913 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } } 7, 62 -- Received: from ([10.32.15.102]) } } 7, 62 -- by imail.umdnj.edu with ESMTP with TLS id 8XSJWG1.87825724; } } 7, 62 -- Fri, 14 Oct 2011 13:26:58 -0400 } } 7, 62 -- MIME-version: 1.0 } } 7, 62 -- Content-transfer-encoding: 7BIT } } 7, 62 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed } } 7, 62 -- Received: from [127.0.0.1] ([10.32.15.102]) } } 7, 62 -- by scpmmp2.umdnj.edu (Oracle Communications Messaging Exchange Server } } 7, 62 -- 7u4-22.01 64bit (built Apr 21 2011)) } } 7, 62 -- with ESMTP id {0LT200H2HGGXOFB0-at-scpmmp2.umdnj.edu} for } } 7, 62 -- microscopy-at-microscopy.com; Fri, 14 Oct 2011 13:26:58 -0400 (EDT) } } 7, 62 -- Message-id: {4E987115.1020809-at-umdnj.edu} } } 7, 62 -- Date: Fri, 14 Oct 2011 13:27:49 -0400 } } 7, 62 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} } } 7, 62 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.22) } } 7, 62 -- Gecko/20110902 Thunderbird/3.1.14 } } 7, 62 -- To: ZZhang-at-uwyo.edu, microscopy-at-microscopy.com } } 7, 62 -- Subject: [Filtered] Re: [Microscopy] digital imaging standards } } 7, 62 -- References: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } } 7, 62 -- In-reply-to: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } } 7, 62 -- X-esp: ESP {11} = } } 7, 62 -- SHA: {0} } } 7, 62 -- SHA_FLAGS: {0} } } 7, 62 -- UHA: {10} } } 7, 62 -- ISC: {0} } } 7, 62 -- BAYES: {0} } } 7, 62 -- SenderID: {0} } } 7, 62 -- DKIM: {0} } } 7, 62 -- TS: {0} } } 7, 62 -- SIG: {} } } 7, 62 -- DSC: {0} } } 7, 62 -- TRU_freehosting: {0} } } 7, 62 -- TRU_misc_spam: {0} } } 7, 62 -- TRU_marketing_spam: {0} } } 7, 62 -- TRU_profanity_spam: {0} } } 7, 62 -- TRU_spam1: {0} } } 7, 62 -- TRU_scam_spam: {0} } } 7, 62 -- TRU_money_spam: {0} } } 7, 62 -- URL Real-Time Signatures: {0} } } 7, 62 -- TRU_watch_spam: {0} } } 7, 62 -- TRU_embedded_image_spam: {1} } } 7, 62 -- TRU_spam2: {0} } } 7, 62 -- TRU_medical_spam: {0} } } 7, 62 -- TRU_playsites: {0} } } 7, 62 -- TRU_html_image_spam: {0} } } 7, 62 -- TRU_stock_spam: {0} } } 7, 62 -- TRU_urllinks: {0} } } 7, 62 -- TRU_adult_spam: {0} } } 7, 62 -- TRU_legal_spam: {0} } } 7, 62 -- TRU_lotto_spam: {0} } } 7, 62 -- TRU_ru_spamsubj: {0} } } 7, 62 -- TRU_phish_spam: {0} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 5, 39 -- From John.Mardinly-at-asu.edu Mon Oct 17 12:32:40 2011 } 5, 39 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) } 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HHWe4A013994 } 5, 39 -- for {Microscopy-at-Microscopy.com} ; Mon, 17 Oct 2011 12:32:40 -0500 } 5, 39 -- X-ASG-Debug-ID: 1318872756-03efbe6e79fce090003-FOsErg } 5, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id FSFmKdpGWqRjP8RZ (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Mon, 17 Oct 2011 10:32:37 -0700 (MST) } 5, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 5, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 } 5, 39 -- X-ASG-Whitelist: Client } 5, 39 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by } 5, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id } 5, 39 -- 8.2.255.0; Mon, 17 Oct 2011 10:32:36 -0700 } 5, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by } 5, 39 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Mon, 17 Oct } 5, 39 -- 2011 10:32:32 -0700 } 5, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu } 5, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Mon, 17 Oct 2011 } 5, 39 -- 17:32:32 +0000 } 5, 39 -- Subject: [Filtered] Re: [Microscopy] Re: digital imaging standards } 5, 39 -- References: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} } 5, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: digital imaging standards } 5, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 5, 39 -- Content-Type: text/plain; charset="us-ascii" } 5, 39 -- In-Reply-To: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} } 5, 39 -- Message-ID: {94BA1879-94B5-4C98-ADE4-0231231FCC76-at-exchange.asu.edu} } 5, 39 -- Thread-Topic: [Microscopy] Re: digital imaging standards } 5, 39 -- Thread-Index: AcyM8sBlFrqgOqJPS1KyooS3hOPGlQ== } 5, 39 -- Date: Mon, 17 Oct 2011 10:37:53 -0700 } 5, 39 -- To: {mcauliff-at-umdnj.edu} , MSA {Microscopy-at-Microscopy.com} } 5, 39 -- CC: {John.Mardinly-at-asu.edu} } 5, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) } 5, 39 -- X-OriginalArrivalTime: 17 Oct 2011 17:32:32.0980 (UTC) FILETIME=[C0B28140:01CC8CF2] } 5, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] } 5, 39 -- X-Barracuda-Start-Time: 1318872757 } 5, 39 -- X-Barracuda-Encrypted: RC4-MD5 } 5, 39 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi } 5, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu } 5, 39 -- Content-Transfer-Encoding: 8bit } 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HHWe4A013994 } ==============================End of - Headers============================== } }
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America E.P. Wigner Fellow - Oak Ridge National Laboratory Senior Fellow of the Computational Institute - University of Chicago President Microscopy Society of America
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 22, 27 -- From zaluzec-at-aaem.amc.anl.gov Mon Oct 17 13:01:18 2011 22, 27 -- Received: from znl.com ([206.69.208.20]) 22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HI1I0W031018; 22, 27 -- Mon, 17 Oct 2011 13:01:18 -0500 22, 27 -- Received: from localhost (localhost [127.0.0.1]) 22, 27 -- by znl.com (Postfix) with ESMTP id 5F08C42A2EE; 22, 27 -- Mon, 17 Oct 2011 13:01:18 -0500 (CDT) 22, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 22, 27 -- Received: from znl.com ([127.0.0.1]) 22, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 22, 27 -- with ESMTP id IzVIzMiGExQr; Mon, 17 Oct 2011 13:01:17 -0500 (CDT) 22, 27 -- Received: from NestorPwrBk.local (unknown [187.126.150.126]) 22, 27 -- by znl.com (Postfix) with ESMTPA id B6F8B42A2E7; 22, 27 -- Mon, 17 Oct 2011 13:01:15 -0500 (CDT) 22, 27 -- Message-ID: {4E9C6D69.6030205-at-aaem.amc.anl.gov} 22, 27 -- Date: Mon, 17 Oct 2011 16:01:13 -0200 22, 27 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 22, 27 -- Organization: ANL 22, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 22, 27 -- MIME-Version: 1.0 22, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 27 -- CC: Nestor_Zaluzec-at-microscopy.com 22, 27 -- Subject: digital imaging standards : JPEG vs Lossless 22, 27 -- References: {201110171732.p9HHWfF3014007-at-ns.microscopy.com} 22, 27 -- In-Reply-To: {201110171732.p9HHWfF3014007-at-ns.microscopy.com} 22, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 22, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There is another important distinction between jpegs and tiffs, apart from the loss of data incurred via jpeg compression. A tiff file retains the information for each color (Red, Green and Blue for RGB; Cyan, Magenta, Yellow and blacK for CMYK) in a separate channel, allowing colors to be balanced and adjusted relative to each other. A jpeg mixes them into a single channel (which is why it's one third or one quarter the size of a tiff), which means they can no longer be treated individually. Nowadays a good jpeg will be accepted by many publishers, particularly for online publication, but for color-critical work most still insist on tiffs.
Paul Callomon Collections Manager in Malacology, Invertebrate Paleontology and General Invertebrates Academy of Natural Sciences 1900 Parkway, Philadelphia, PA 19103-1195, USA callomon-at-ansp.org Tel. 215-405-5096
==============================Original Headers============================== 5, 21 -- From callomon-at-ansp.org Mon Oct 17 13:47:29 2011 5, 21 -- Received: from mail.ansp.org (mail.ansp.org [192.204.19.65]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HIlTNR016079 5, 21 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 13:47:29 -0500 5, 21 -- Received: from mail.ansp.org ([fe80::b55d:262c:e9e6:c197]) by mail.ansp.org 5, 21 -- ([fe80::b55d:262c:e9e6:c197%11]) with mapi; Mon, 17 Oct 2011 14:48:40 -0400 5, 21 -- From: Paul Callomon {callomon-at-ansp.org} 5, 21 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 5, 21 -- Subject: Jpeg vs tiff 5, 21 -- Thread-Topic: Jpeg vs tiff 5, 21 -- Thread-Index: AcyM/WIHPrPqZxdrQlCPMq7LijaNRQ== 5, 21 -- Date: Mon, 17 Oct 2011 18:48:39 +0000 5, 21 -- Message-ID: {A239B1CB55E7924E955AB07041E7185926D149AD-at-mail.ansp.org} 5, 21 -- Accept-Language: en-US 5, 21 -- Content-Language: en-US 5, 21 -- X-MS-Has-Attach: 5, 21 -- X-MS-TNEF-Correlator: 5, 21 -- Content-Type: text/plain; charset="us-ascii" 5, 21 -- MIME-Version: 1.0 5, 21 -- Content-Transfer-Encoding: 8bit 5, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HIlTNR016079 ==============================End of - Headers==============================
Thank you all for your response to my early post "digital imaging standard".
Although the question was very broad and vague, I received many responses that are very helpful! Many of the responses point to guidelines, rather than "standards", but they are important for the discussion.
Here is a long summary and I hope this can stimulate more discussion (and research?) to this exciting (and confusing) topic. Two more related questions are posted at the end.
------------------------------------------------ SUMMARY: The standards for digital imaging (especially for microscopy imaging) could include, at least, the following aspects:
Acquisition/Capturing (including digitization of non-digital images?); Storage; Processing/manipulation/analysis/ Digital imaging ethics; Image output
1. Acquisition/capturing
1a: the health of the microscope:
--------------- John Oreopoulos [john.oreopoulos-at-UTORONTO.CA]
There are several different standards for measuring spatial resolution: sub-diffraction sized fluorescent beads, mirrors, etc. Fluorescent beads can be purchased from many different companies, but there is no agreed upon standard size, dye spectra, or anything like that. Some people have used flow cytometry beads before, and I think the quality control is a bit tighter on those.
Molecular Probes also sells a fluorescent bead kit with beads that step up their fluorescent intensity in known amounts (no commercial interest):
Pawley's confocal handbook says that you can coat these with a fluorescent dye if you want to use them for fluorescence.
Unfortunately, there is no intensity standard or "standard candle" for microscopy, although as you may have seen in some recent posts, some people suggest using the transmitted light source of the microscope and a power meter. I think uranyl glass has been talked about several times as well. If someone does have a good intensity standard, I'd really like to know about it as well.
----------------- Rich Cole [rcole-at-WADSWORTH.ORG] and Anda Cornea [corneaan-at-OHSU.EDU]
ABRF (The Association of Biomolecular Resource Facilities) studies http://www.abrf.org/index.cfm/group.show/LightMicroscopyResearchGroup.54.htm
Stack, R., Bayles, C., Girard, A., Martin, K., Opansky, C., Schulz, K., and Cole, R. (2011) Quality Assurance Testing for Modern Optical Imaging Systems. Microscopy & Microanalysis 17(4):598-606.
They use Chroma fluorescent plastic slides, sub-resolution beads, orange/orange spectral separation beads and reflection off gold coated slide. All good, none perfect.
For the health of my SP5 AOBS, I run a daily a 3 minute test that involves x-z-lambda, (occasionally x-z-lambda-t) scanning in reflection mode off a plain glass slide with all lasers on, same parameters of course. It gives a lot of information and it makes it relatively easy to detect problems and pinpoint their sources.
1b: Image acquisition/capturing
JOEL B. SHEFFIELD [jbs-at-TEMPLE.EDU]
There may not be formal standards, but I would certainly think that there are principles that should guide digital microscopy.
1. The pixel density should correspond, at a minimum, to the relevant detail in the original source.
2. The bit depth should include all levels of intensity in the original --i.e. not saturated, and not clipped.
3. For microscopy, images should include a magnification standard.
JPEG formating CHANGES the data. What is even worse if you take a JPEG image and store it a second time CHANGES are made to the last set of CHANGES. This corrupts the integrity of the scientific data.
Do a simple test. Take an image store it in TIFF.
Then store it as JPEG, then open the JPEG and compare the results pixel by pixel. You no longer have the same information. If you then store the JPEG again the compression routine reformat the data yet again.
You should always, repeat always, store your original data in a lossless format. RAW, TIFF are two of the examples. You can just JPEG to post on the WWW or print posters, but never when any quantitative work is being done from that data set.
2. Storage and image analysis ------------------- Geoff McAuliffe [mcauliff-at-umdnj.edu]
Do not use a "lossy" compression, ie. JPEG. Use TIFF to save your images. Journals will publish guidelines in the "Instructions to
Authors" section as to what, if any, modifications may be allowed. Always save the original, unaltered image file and a backup. ----------------------- Jennifer Hill [jjhill100-at-gmail.com]
In my experiences, most researchers use a tiff file format for publications and lossless compression jpegs for rough review. As far as a standard goes, there should be no manipulation in photoshop or similar programs that alters the original specimen from its original state. Adjusting brightness/contrast is usually ok; and depending on the purpose/ final output of the image the background can be cleaned of dust/noise. I'd be happy to answer any more of your questions in regards to imaging.
Ps. I agree with the other individual who posted; a scale of magnification should be included with the image.
-------------------- Jim Quinn [jquinn11733-at-gmail.com]
There are many ASTM standards for microscopy, some are digital, such as E1122 for JK inclusion rating by automated image analysis. E2765 for image capture and storage. The E4 subcommittee chair is John Friel. He can likely point you in the correct direction.
-------------------- Donnelly, Tom [TDonnelly-at-api.com]
As far as standards for mfg. there really is nothing out there. There is an industry group, OPIA, that meets one or two times per year to discuss "standards". Unfortunately each of the "Big Four" are all for standards for data files, operational communication, etc. as long as the standards conform to their individual formats.
The smaller companies which create software and third party hardware, as a group would support across the board standardization because every time Zeiss, Leica, Nikon, or Olympus change a hardware design or a software data input/output the third party vendors have to redesign their products.
This conflict will probably prevent true standards for a long time.
The best chance for data standards, at least, is the Open Microscopy Environment, project. http://www.openmicroscopy.org/site This project allows imaging facilities to utilize a single data format for analysis and storage.
------------------------------- Dmitry Sokolov [dmitry.v.sokolov-at-GMAIL.COM] MIAWiki, Wiki for Microscopy and Image Analysis, is a collaboration tool where primary information like papers, patents, etc. can be cited, analysed, published and shared for free. In many cases R&D is based on the best practices and protocols rather than standards.
Please discover capabilities of MIAWiki of real time accumulation and finding knowledge on your current demand.
----------------------- Stan Schwartz [nikonbio-at-gmail.com]
The International Organization for Standardization covers this. http://www.iso.org/iso/search.htm?qt=digital+image&sort=rel&type=simple&published=on
Along with many standards for Microscopy. http://www.iso.org/iso/search.htm?qt=microscopy&sort=rel&type=simple&published=on
NOTE: The documents can be only access on a pay basis. There is not even a summary available.
3. Digital imaging ethics ------------------------- John Oreopoulos [john.oreopoulos-at-UTORONTO.CA]
there is a fantastic article by Douglas Cromey that thoroughly discusses the topic:
Cromey, D., Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images. Science and Engineering Ethics, 2010.
------------------------- Mike Bode [mike.bode-at-resaltatech.com]
I don't think there is a standard as to what file format to use or what image processing software to use, but there are ethical guidelines as to what can be done and what should not be done, and how the image manipulations should be documented. One such article you can find here:
The gist of both is that you need to stay as true as possible to the original image and pixel data (no compression), and that anything that could lead to artifacts needs to be documented, and, of course, that whatever you do to the image is documented in such a fashion that it can be repeated (as all scientific data should).
-------------------- 4. Image output
Are we still reined by the mercy of the publishers? Often I see publishers require a resolution of 1200ppi, but no mention the image size. It ends up a "high resolution" image but the print size is 0.01 inches. Any thought on that?
Further questions:
1. Remember in the "old" days, that all films have a speed, ISO 100, 200 etc. Nowadays, do we just use the "gain" to control the speed and "grain size" on the digital camera? Is there a "standard"?
2. Quantitative analysis is becoming a norm of digital imaging. Does it always require 16bit images, rather 8bit? Did anyone compare the same sample using 8bit or 16bit, and see a significant difference?
Zhaojie
Zhaojie Zhang, Ph. D. Director, Jenkins Microscopy Facility University of Wyoming Laramie, WY 82071 PHONE: 307-766-3038 FAX: 307-766-5625
==============================Original Headers============================== 72, 28 -- From ZZhang-at-uwyo.edu Mon Oct 17 13:51:43 2011 72, 28 -- Received: from aspensprings.uwyo.edu (aspensprings.uwyo.edu [129.72.10.32]) 72, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HIphwl023121 72, 28 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 13:51:43 -0500 72, 28 -- Received: from ponyexpress-ht4.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) 72, 28 -- by aspensprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9HIpdvY018942 72, 28 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL) 72, 28 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 12:51:39 -0600 (MDT) 72, 28 -- (envelope-from ZZhang-at-uwyo.edu) 72, 28 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by 72, 28 -- ponyexpress-ht4.uwyo.edu ([fe80::8de7:b225:a85b:d0ee%13]) with mapi id 72, 28 -- 14.01.0289.001; Mon, 17 Oct 2011 12:51:39 -0600 72, 28 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} 72, 28 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} 72, 28 -- Subject: digital imaging standard - Summary and further questions 72, 28 -- Thread-Topic: digital imaging standard - Summary and further questions 72, 28 -- Thread-Index: AQHMjP0jFIRVVkAYzECgj19hPHOZhpWA4J9A 72, 28 -- Date: Mon, 17 Oct 2011 18:51:38 +0000 72, 28 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D4285A3-at-ponyexpress-m7.uwyo.edu} 72, 28 -- Accept-Language: en-US 72, 28 -- Content-Language: en-US 72, 28 -- X-MS-Has-Attach: 72, 28 -- X-MS-TNEF-Correlator: 72, 28 -- x-originating-ip: [10.56.56.50] 72, 28 -- Content-Type: text/plain; charset="us-ascii" 72, 28 -- MIME-Version: 1.0 72, 28 -- Content-Transfer-Encoding: 8bit 72, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HIphwl023121 ==============================End of - Headers==============================
I think it all depends on the intended future use.
I wouldn't go back and do fractal or fourier analysis on a JPEG image; but I would use JPEGs for basic reports.
Also, for Forensic Investigation, TIFF or RAW format is certainly better and implied as a first basis of collection in say -
ASTM E2765 - 11 Standard Practice for Use of Image Capture and Storage Technology in Forensic Document Examination
I don't recall the exact discussions when the language in this standard was developed (I did have some input) but purpose was a part of it. Some considerations discussed in the standard are useful to review but the level of scrutiny may vary and should probably be set as a policy basis. Perhaps there should be a standard on "levels of data image quality" and then one could state what the image value is in general and decide on its usefulness for a particular task.
Here we vary our quality depending on the project. Routine stuff is JPEG (mod-high relative quality) to Litigation (RAW & TIFF both stored with each change documented).
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123 www.ph2llc.com (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Monday, October 17, 2011 2:04 PM To: ph2-at-sprynet.com
John
You are missing an important point.
By human eye you may not be able distinguish a JPEG from a TIFF.
However our images are scientific data, and we end up doing quantitative analysis on this data.
JPEG formating CHANGES the data. What is even worse if you take a JPEG image and store it a second time CHANGES are made to the last set of CHANGES. This corrupts the integrity of the scientific data.
Do a simple test. Take an image store it in TIFF. Then store it as JPEG, then open the JPEG and compare the results pixel by pixel. You no longer have the same information. If you then store the JPEG again the compression routine reformat the data yet again.
You should always, repeat always, store your original data in a lossless format. RAW, TIFF are two of the examples. You can just JPEG to post on the WWW or print posters, but never when any quantitative work is being done from that data set.
Nestor (steps down from his soap box) Your Friendly Neighborhood SysOp
On 10/17/11 3:32 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I beg to differ-JPEG is nearly indistinguishable from TIFF for photographic reproduction. That is one reason why all of the digital cameras in the world default to JPEG, even the expensive digital SLR's. The only place in a micrograph where you can actually discern compression artifacts is around micron markers or other text, and even that is only visible by zooming in on the region. BTW, as soon as you insert that image into PowerPoint for a report or presentation, it is automatically compressed anyway. However, I do agree that one should archive the raw original DM or whatever format the camera produces. } } John Mardinly } ASU } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Do not use a "lossy" compression, ie. JPEG. Use TIFF to save your } } images. Journals will publish guidelines in the "Instructions to } } Authors" section as to what, if any, modifications may be allowed. } } Always save the original, unaltered image file and a backup. } } } } Geoff } } } } On 10/13/2011 7:29 PM, ZZhang-at-uwyo.edu wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Does anyone know if there is a "standard" for digital imaging, especially for microscopy related digital imaging? } } } } } } If so, who is in charge of making such a standard? } } } } } } Thank you, } } } } } } } } } Zhaojie Zhang, Ph. D. } } } Director, Jenkins Microscopy Facility } } } University of Wyoming } } } Laramie, WY 82071 } } } PHONE: 307-766-3038 } } } FAX: 307-766-5625 } } } } } } } } } } } } } } } } } } } } } } } } ==============================Original Headers============================== } } } 11, 31 -- From ZZhang-at-uwyo.edu Thu Oct 13 18:28:42 2011 } } } 11, 31 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) } } } 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DNSfu2012687 } } } 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 18:28:41 -0500 } } } 11, 31 -- Received: from ponyexpress-ht3.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) } } } 11, 31 -- by willowsprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9DNSbR7029726 } } } 11, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); } } } 11, 31 -- Thu, 13 Oct 2011 17:28:37 -0600 (MDT) } } } 11, 31 -- (envelope-from ZZhang-at-uwyo.edu) } } } 11, 31 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by } } } 11, 31 -- ponyexpress-ht3.uwyo.edu ([fe80::dc63:d646:8a62:d32f%13]) with mapi id } } } 11, 31 -- 14.01.0289.001; Thu, 13 Oct 2011 17:28:37 -0600 } } } 11, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } } } 11, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} , } } } 11, 31 -- "CONFOCALMICROSCOPY-at-LISTS.UMN.EDU" {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} } } } 11, 31 -- Subject: [Filtered] digital imaging standards } } } 11, 31 -- Thread-Topic: digital imaging standards } } } 11, 31 -- Thread-Index: AQHMif/VsONGQEb1nki2DYxCNhzWww== } } } 11, 31 -- Date: Thu, 13 Oct 2011 23:28:37 +0000 } } } 11, 31 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D41AFBB-at-ponyexpress-m7.uwyo.edu} } } } 11, 31 -- References: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } } 11, 31 -- In-Reply-To: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } } 11, 31 -- Accept-Language: en-US } } } 11, 31 -- Content-Language: en-US } } } 11, 31 -- X-MS-Has-Attach: } } } 11, 31 -- X-MS-TNEF-Correlator: } } } 11, 31 -- x-originating-ip: [10.56.56.50] } } } 11, 31 -- Content-Type: text/plain; charset="us-ascii" } } } 11, 31 -- MIME-Version: 1.0 } } } 11, 31 -- Content-Transfer-Encoding: 8bit } } } 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DNSfu2012687 } } } ==============================End of - Headers============================== } } } } } } } } } } } } -- } } -- } } ********************************************** } } Geoff McAuliffe, Ph.D. } } Neuroscience and Cell Biology } } Robert Wood Johnson Medical School } } 675 Hoes Lane, Piscataway, NJ 08854 } } voice: (732)-235-4583 } } mcauliff-at-umdnj.edu } } ********************************************** } } } } } } } } ==============================Original Headers============================== } } 7, 62 -- From mcauliff-at-umdnj.edu Fri Oct 14 12:27:03 2011 } } 7, 62 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) } } 7, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9EHR3cc001954 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 12:27:03 -0500 } } 7, 62 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) } } 7, 62 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E27A221CF927 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } } 7, 62 -- Received: from umdnj.edu (unknown [10.32.15.102]) } } 7, 62 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 661E621CF913 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } } 7, 62 -- Received: from ([10.32.15.102]) } } 7, 62 -- by imail.umdnj.edu with ESMTP with TLS id 8XSJWG1.87825724; } } 7, 62 -- Fri, 14 Oct 2011 13:26:58 -0400 } } 7, 62 -- MIME-version: 1.0 } } 7, 62 -- Content-transfer-encoding: 7BIT } } 7, 62 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed } } 7, 62 -- Received: from [127.0.0.1] ([10.32.15.102]) } } 7, 62 -- by scpmmp2.umdnj.edu (Oracle Communications Messaging Exchange Server } } 7, 62 -- 7u4-22.01 64bit (built Apr 21 2011)) } } 7, 62 -- with ESMTP id {0LT200H2HGGXOFB0-at-scpmmp2.umdnj.edu} for } } 7, 62 -- microscopy-at-microscopy.com; Fri, 14 Oct 2011 13:26:58 -0400 (EDT) } } 7, 62 -- Message-id: {4E987115.1020809-at-umdnj.edu} } } 7, 62 -- Date: Fri, 14 Oct 2011 13:27:49 -0400 } } 7, 62 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} } } 7, 62 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.22) } } 7, 62 -- Gecko/20110902 Thunderbird/3.1.14 } } 7, 62 -- To: ZZhang-at-uwyo.edu, microscopy-at-microscopy.com } } 7, 62 -- Subject: [Filtered] Re: [Microscopy] digital imaging standards } } 7, 62 -- References: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } } 7, 62 -- In-reply-to: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } } 7, 62 -- X-esp: ESP {11} = } } 7, 62 -- SHA: {0} } } 7, 62 -- SHA_FLAGS: {0} } } 7, 62 -- UHA: {10} } } 7, 62 -- ISC: {0} } } 7, 62 -- BAYES: {0} } } 7, 62 -- SenderID: {0} } } 7, 62 -- DKIM: {0} } } 7, 62 -- TS: {0} } } 7, 62 -- SIG: {} } } 7, 62 -- DSC: {0} } } 7, 62 -- TRU_freehosting: {0} } } 7, 62 -- TRU_misc_spam: {0} } } 7, 62 -- TRU_marketing_spam: {0} } } 7, 62 -- TRU_profanity_spam: {0} } } 7, 62 -- TRU_spam1: {0} } } 7, 62 -- TRU_scam_spam: {0} } } 7, 62 -- TRU_money_spam: {0} } } 7, 62 -- URL Real-Time Signatures: {0} } } 7, 62 -- TRU_watch_spam: {0} } } 7, 62 -- TRU_embedded_image_spam: {1} } } 7, 62 -- TRU_spam2: {0} } } 7, 62 -- TRU_medical_spam: {0} } } 7, 62 -- TRU_playsites: {0} } } 7, 62 -- TRU_html_image_spam: {0} } } 7, 62 -- TRU_stock_spam: {0} } } 7, 62 -- TRU_urllinks: {0} } } 7, 62 -- TRU_adult_spam: {0} } } 7, 62 -- TRU_legal_spam: {0} } } 7, 62 -- TRU_lotto_spam: {0} } } 7, 62 -- TRU_ru_spamsubj: {0} } } 7, 62 -- TRU_phish_spam: {0} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 5, 39 -- From John.Mardinly-at-asu.edu Mon Oct 17 12:32:40 2011 } 5, 39 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) } 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HHWe4A013994 } 5, 39 -- for {Microscopy-at-Microscopy.com} ; Mon, 17 Oct 2011 12:32:40 -0500 } 5, 39 -- X-ASG-Debug-ID: 1318872756-03efbe6e79fce090003-FOsErg } 5, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id FSFmKdpGWqRjP8RZ (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Mon, 17 Oct 2011 10:32:37 -0700 (MST) } 5, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 5, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 } 5, 39 -- X-ASG-Whitelist: Client } 5, 39 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by } 5, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id } 5, 39 -- 8.2.255.0; Mon, 17 Oct 2011 10:32:36 -0700 } 5, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by } 5, 39 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Mon, 17 Oct } 5, 39 -- 2011 10:32:32 -0700 } 5, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu } 5, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Mon, 17 Oct 2011 } 5, 39 -- 17:32:32 +0000 } 5, 39 -- Subject: [Filtered] Re: [Microscopy] Re: digital imaging standards } 5, 39 -- References: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} } 5, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: digital imaging standards } 5, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 5, 39 -- Content-Type: text/plain; charset="us-ascii" } 5, 39 -- In-Reply-To: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} } 5, 39 -- Message-ID: {94BA1879-94B5-4C98-ADE4-0231231FCC76-at-exchange.asu.edu} } 5, 39 -- Thread-Topic: [Microscopy] Re: digital imaging standards } 5, 39 -- Thread-Index: AcyM8sBlFrqgOqJPS1KyooS3hOPGlQ== } 5, 39 -- Date: Mon, 17 Oct 2011 10:37:53 -0700 } 5, 39 -- To: {mcauliff-at-umdnj.edu} , MSA {Microscopy-at-Microscopy.com} } 5, 39 -- CC: {John.Mardinly-at-asu.edu} } 5, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) } 5, 39 -- X-OriginalArrivalTime: 17 Oct 2011 17:32:32.0980 (UTC) FILETIME=[C0B28140:01CC8CF2] } 5, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] } 5, 39 -- X-Barracuda-Start-Time: 1318872757 } 5, 39 -- X-Barracuda-Encrypted: RC4-MD5 } 5, 39 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi } 5, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu } 5, 39 -- Content-Transfer-Encoding: 8bit } 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HHWe4A013994 } ==============================End of - Headers============================== } }
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America E.P. Wigner Fellow - Oak Ridge National Laboratory Senior Fellow of the Computational Institute - University of Chicago President Microscopy Society of America
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 22, 27 -- From zaluzec-at-aaem.amc.anl.gov Mon Oct 17 13:01:18 2011 22, 27 -- Received: from znl.com ([206.69.208.20]) 22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HI1I0W031018; 22, 27 -- Mon, 17 Oct 2011 13:01:18 -0500 22, 27 -- Received: from localhost (localhost [127.0.0.1]) 22, 27 -- by znl.com (Postfix) with ESMTP id 5F08C42A2EE; 22, 27 -- Mon, 17 Oct 2011 13:01:18 -0500 (CDT) 22, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 22, 27 -- Received: from znl.com ([127.0.0.1]) 22, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 22, 27 -- with ESMTP id IzVIzMiGExQr; Mon, 17 Oct 2011 13:01:17 -0500 (CDT) 22, 27 -- Received: from NestorPwrBk.local (unknown [187.126.150.126]) 22, 27 -- by znl.com (Postfix) with ESMTPA id B6F8B42A2E7; 22, 27 -- Mon, 17 Oct 2011 13:01:15 -0500 (CDT) 22, 27 -- Message-ID: {4E9C6D69.6030205-at-aaem.amc.anl.gov} 22, 27 -- Date: Mon, 17 Oct 2011 16:01:13 -0200 22, 27 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 22, 27 -- Organization: ANL 22, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 22, 27 -- MIME-Version: 1.0 22, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 27 -- CC: Nestor_Zaluzec-at-microscopy.com 22, 27 -- Subject: digital imaging standards : JPEG vs Lossless 22, 27 -- References: {201110171732.p9HHWfF3014007-at-ns.microscopy.com} 22, 27 -- In-Reply-To: {201110171732.p9HHWfF3014007-at-ns.microscopy.com} 22, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 22, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
while I agree that JPEG is not a suitable format to store quantitative data, there is one little issue about TIF files I'd like to raise:
TIFFs are actually very flexible containers for all kinds of image data with differences in channels (RGB, CMYK, Alpha), pixel depth (4, 8, 16, 32, ... bit), layers etc ... and also compression. There are TIFFs out there that have no compression, others with lossless compression and even some with lossy compression, similar to JPEGs.
Hence, it is important to know how to save a TIF for later analysis ... if in doubt, uncompressed or LZW.
Best,
Guenter
-- Dr. Guenter Resch, IMP-IMBA-GMI Electron Microscopy Facility Email: guenter.resch-at-imba.oeaw.ac.at; Web: http://cores.imp.ac.at/em Phone: +43 (1) 79044-4250; Fax/Voice Mail: +43 (1) 79044-224250 Post: Dr. Bohr-Gasse 3, 1030 Vienna, Austria, European Union
==============================Original Headers============================== 7, 26 -- From guenter.resch-at-imba.oeaw.ac.at Mon Oct 17 14:33:41 2011 7, 26 -- Received: from mail.imp.ac.at (mail.imp.ac.at [193.171.188.9]) 7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HJXemr000744 7, 26 -- for {microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 14:33:40 -0500 7, 26 -- Received: from impexchfront.imp.univie.ac.at (impexchfront.imp.univie.ac.at [10.42.40.110]) 7, 26 -- by mail.imp.ac.at (Postfix) with ESMTP id DA928182198 7, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 21:33:34 +0200 (CEST) 7, 26 -- Received: from Aventine (80.108.26.214) by webmail.imp.univie.ac.at 7, 26 -- (10.42.40.147) with Microsoft SMTP Server (TLS) id 8.3.213.0; Mon, 17 Oct 7, 26 -- 2011 21:33:34 +0200 7, 26 -- Date: Mon, 17 Oct 2011 21:33:32 +0200 7, 26 -- From: Guenter Resch {guenter.resch-at-imba.oeaw.ac.at} 7, 26 -- To: {Microscopy-at-microscopy.com} 7, 26 -- Subject: Re: [Microscopy] Re: digital imaging standards 7, 26 -- Message-ID: {20111017213332.458a9a32-at-Aventine} 7, 26 -- In-Reply-To: {201110141736.p9EHajbK014773-at-ns.microscopy.com} 7, 26 -- References: {201110141736.p9EHajbK014773-at-ns.microscopy.com} 7, 26 -- X-Mailer: Claws Mail 3.7.9 (GTK+ 2.24.6; i686-pc-linux-gnu) 7, 26 -- MIME-Version: 1.0 7, 26 -- Content-Type: text/plain; charset="US-ASCII" 7, 26 -- Content-Transfer-Encoding: 7bit 7, 26 -- X-IMP-MailScanner-Information: Please contact the ISP for more information 7, 26 -- X-IMP-MailScanner-ID: DA928182198.A1E62 7, 26 -- X-IMP-MailScanner: Found to be clean 7, 26 -- X-IMP-MailScanner-From: guenter.resch-at-imba.oeaw.ac.at 7, 26 -- X-Spam-Status: No ==============================End of - Headers==============================
I I don't think that you and Nestor (or me) are in disagreement. As soon as you print an image it doesn't' really matter if you use JPEG or TIFF. The printing process will likely create more artifacts than a reasonable JPEG compression. However, once you have acquired an image and stored it with a lossy format it is no longer amenable to many image processing steps that might be necessary to extract the information that is needed. And that may include steps that you didn't think of when you saved the image initially. Look at it this way: Would you store the quantitative results of any experiment in a way that ensures that the data degrades? Of course not. An image should be treated the same way. As soon as you acquire it, it should be saved in way that does not degrade the data. Since any lossy compression does lead to a deterioration, it should not be used. Of course, making a copy and save it as a jpeg for printing is probably OK. I don't think that anybody would scan such an image from a magazine and then assume that they have recovered the original data.
There was a very intense discussion about this a couple of years back w/r to 21 cfr rule 11, where the FDA tried to implement a way to make sure that data, including images, are available at a later stage to verify results. I am sure this will come up again at some point. They also came to the conclusion that the original data must be saved in a way that they can be recovered. A lossy compression will not allow that.
And why take the risk? We now have TB sized hard disks that can store 10s of thousands of images at very low prices.
Mike Bode, Ph.D. ResAlta Research Technologies Corp.
-----Original Message----- X-from: ph2-at-sprynet.com [mailto:ph2-at-sprynet.com] Sent: Monday, October 17, 2011 1:04 PM To: mike.bode-at-resaltatech.com
Nestor:
I think it all depends on the intended future use.
I wouldn't go back and do fractal or fourier analysis on a JPEG image; but I would use JPEGs for basic reports.
Also, for Forensic Investigation, TIFF or RAW format is certainly better and implied as a first basis of collection in say -
ASTM E2765 - 11 Standard Practice for Use of Image Capture and Storage Technology in Forensic Document Examination
I don't recall the exact discussions when the language in this standard was developed (I did have some input) but purpose was a part of it. Some considerations discussed in the standard are useful to review but the level of scrutiny may vary and should probably be set as a policy basis. Perhaps there should be a standard on "levels of data image quality" and then one could state what the image value is in general and decide on its usefulness for a particular task.
Here we vary our quality depending on the project. Routine stuff is JPEG (mod-high relative quality) to Litigation (RAW & TIFF both stored with each change documented).
Tony
...................................................................... Andrew Anthony "Tony" Havics, CHMM, CIH, PE pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123 www.ph2llc.com (317) 718-7020 off (317) 718-7038 fax (317) 409-3238 cell
90% of Risk Management is knowing where to place the decimal point...any consultant can give you the other 10%(SM)
This message is from pH2. This message and any attachments may contain legally privileged or confidential information, and are intended only for the individual or entity identified above as the addressee. If you are not the addressee, or if this message has been addressed to you in error, you are not authorized to read, copy, or distribute this message and any attachments, and we ask that you please delete this message and attachments (including all copies) and notify the sender by return e-mail or by phone at 317-718-7020. Delivery of this message and any attachments to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or a privilege. All personal messages express views only of the sender, which are not to be attributed to pH2 and may not be copied or distributed without this statement.
-----Original Message----- X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov] Sent: Monday, October 17, 2011 2:04 PM To: ph2-at-sprynet.com
John
You are missing an important point.
By human eye you may not be able distinguish a JPEG from a TIFF.
However our images are scientific data, and we end up doing quantitative analysis on this data.
JPEG formating CHANGES the data. What is even worse if you take a JPEG image and store it a second time CHANGES are made to the last set of CHANGES. This corrupts the integrity of the scientific data.
Do a simple test. Take an image store it in TIFF. Then store it as JPEG, then open the JPEG and compare the results pixel by pixel. You no longer have the same information. If you then store the JPEG again the compression routine reformat the data yet again.
You should always, repeat always, store your original data in a lossless format. RAW, TIFF are two of the examples. You can just JPEG to post on the WWW or print posters, but never when any quantitative work is being done from that data set.
Nestor (steps down from his soap box) Your Friendly Neighborhood SysOp
On 10/17/11 3:32 PM, John.Mardinly-at-asu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I beg to differ-JPEG is nearly indistinguishable from TIFF for photographic reproduction. That is one reason why all of the digital cameras in the world default to JPEG, even the expensive digital SLR's. The only place in a micrograph where you can actually discern compression artifacts is around micron markers or other text, and even that is only visible by zooming in on the region. BTW, as soon as you insert that image into PowerPoint for a report or presentation, it is automatically compressed anyway. However, I do agree that one should archive the raw original DM or whatever format the camera produces. } } John Mardinly } ASU } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Do not use a "lossy" compression, ie. JPEG. Use TIFF to save your } } images. Journals will publish guidelines in the "Instructions to } } Authors" section as to what, if any, modifications may be allowed. } } Always save the original, unaltered image file and a backup. } } } } Geoff } } } } On 10/13/2011 7:29 PM, ZZhang-at-uwyo.edu wrote: } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Does anyone know if there is a "standard" for digital imaging, especially for microscopy related digital imaging? } } } } } } If so, who is in charge of making such a standard? } } } } } } Thank you, } } } } } } } } } Zhaojie Zhang, Ph. D. } } } Director, Jenkins Microscopy Facility } } } University of Wyoming } } } Laramie, WY 82071 } } } PHONE: 307-766-3038 } } } FAX: 307-766-5625 } } } } } } } } } } } } } } } } } } } } } } } } ==============================Original Headers============================== } } } 11, 31 -- From ZZhang-at-uwyo.edu Thu Oct 13 18:28:42 2011 } } } 11, 31 -- Received: from willowsprings.uwyo.edu (willowsprings.uwyo.edu [129.72.10.31]) } } } 11, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9DNSfu2012687 } } } 11, 31 -- for {microscopy-at-microscopy.com} ; Thu, 13 Oct 2011 18:28:41 -0500 } } } 11, 31 -- Received: from ponyexpress-ht3.uwyo.edu (extlb.uwyo.edu [172.26.4.4]) } } } 11, 31 -- by willowsprings.uwyo.edu (8.14.4/8.14.4) with ESMTP id p9DNSbR7029726 } } } 11, 31 -- (version=TLSv1/SSLv3 cipher=AES128-SHA bits=128 verify=FAIL); } } } 11, 31 -- Thu, 13 Oct 2011 17:28:37 -0600 (MDT) } } } 11, 31 -- (envelope-from ZZhang-at-uwyo.edu) } } } 11, 31 -- Received: from ponyexpress-m7.uwyo.edu ([fe80::105c:cf9a:a192:4b54]) by } } } 11, 31 -- ponyexpress-ht3.uwyo.edu ([fe80::dc63:d646:8a62:d32f%13]) with mapi id } } } 11, 31 -- 14.01.0289.001; Thu, 13 Oct 2011 17:28:37 -0600 } } } 11, 31 -- From: "Z.J. Zhang" {ZZhang-at-uwyo.edu} } } } 11, 31 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com} , } } } 11, 31 -- "CONFOCALMICROSCOPY-at-LISTS.UMN.EDU" {CONFOCALMICROSCOPY-at-LISTS.UMN.EDU} } } } 11, 31 -- Subject: [Filtered] digital imaging standards } } } 11, 31 -- Thread-Topic: digital imaging standards } } } 11, 31 -- Thread-Index: AQHMif/VsONGQEb1nki2DYxCNhzWww== } } } 11, 31 -- Date: Thu, 13 Oct 2011 23:28:37 +0000 } } } 11, 31 -- Message-ID: {EF8DB51D155E974C8F9D33968898545D41AFBB-at-ponyexpress-m7.uwyo.edu} } } } 11, 31 -- References: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } } 11, 31 -- In-Reply-To: {201110132156.p9DLu73T032646-at-ns.microscopy.com} } } } 11, 31 -- Accept-Language: en-US } } } 11, 31 -- Content-Language: en-US } } } 11, 31 -- X-MS-Has-Attach: } } } 11, 31 -- X-MS-TNEF-Correlator: } } } 11, 31 -- x-originating-ip: [10.56.56.50] } } } 11, 31 -- Content-Type: text/plain; charset="us-ascii" } } } 11, 31 -- MIME-Version: 1.0 } } } 11, 31 -- Content-Transfer-Encoding: 8bit } } } 11, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9DNSfu2012687 } } } ==============================End of - Headers============================== } } } } } } } } } } } } -- } } -- } } ********************************************** } } Geoff McAuliffe, Ph.D. } } Neuroscience and Cell Biology } } Robert Wood Johnson Medical School } } 675 Hoes Lane, Piscataway, NJ 08854 } } voice: (732)-235-4583 } } mcauliff-at-umdnj.edu } } ********************************************** } } } } } } } } ==============================Original Headers============================== } } 7, 62 -- From mcauliff-at-umdnj.edu Fri Oct 14 12:27:03 2011 } } 7, 62 -- Received: from zix03.umdnj.edu (zix03.UMDNJ.EDU [130.219.34.126]) } } 7, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9EHR3cc001954 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 12:27:03 -0500 } } 7, 62 -- Received: from zix03.umdnj.edu (ZixVPM [127.0.0.1]) } } 7, 62 -- by Outbound.umdnj.edu (Proprietary) with ESMTP id E27A221CF927 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } } 7, 62 -- Received: from umdnj.edu (unknown [10.32.15.102]) } } 7, 62 -- by zix03.umdnj.edu (Proprietary) with ESMTP id 661E621CF913 } } 7, 62 -- for {microscopy-at-microscopy.com} ; Fri, 14 Oct 2011 13:27:02 -0400 (EDT) } } 7, 62 -- Received: from ([10.32.15.102]) } } 7, 62 -- by imail.umdnj.edu with ESMTP with TLS id 8XSJWG1.87825724; } } 7, 62 -- Fri, 14 Oct 2011 13:26:58 -0400 } } 7, 62 -- MIME-version: 1.0 } } 7, 62 -- Content-transfer-encoding: 7BIT } } 7, 62 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed } } 7, 62 -- Received: from [127.0.0.1] ([10.32.15.102]) } } 7, 62 -- by scpmmp2.umdnj.edu (Oracle Communications Messaging Exchange Server } } 7, 62 -- 7u4-22.01 64bit (built Apr 21 2011)) } } 7, 62 -- with ESMTP id {0LT200H2HGGXOFB0-at-scpmmp2.umdnj.edu} for } } 7, 62 -- microscopy-at-microscopy.com; Fri, 14 Oct 2011 13:26:58 -0400 (EDT) } } 7, 62 -- Message-id: {4E987115.1020809-at-umdnj.edu} } } 7, 62 -- Date: Fri, 14 Oct 2011 13:27:49 -0400 } } 7, 62 -- From: Geoff McAuliffe {mcauliff-at-umdnj.edu} } } 7, 62 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.22) } } 7, 62 -- Gecko/20110902 Thunderbird/3.1.14 } } 7, 62 -- To: ZZhang-at-uwyo.edu, microscopy-at-microscopy.com } } 7, 62 -- Subject: [Filtered] Re: [Microscopy] digital imaging standards } } 7, 62 -- References: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } } 7, 62 -- In-reply-to: {201110132329.p9DNTSNC013931-at-ns.microscopy.com} } } 7, 62 -- X-esp: ESP {11} = } } 7, 62 -- SHA: {0} } } 7, 62 -- SHA_FLAGS: {0} } } 7, 62 -- UHA: {10} } } 7, 62 -- ISC: {0} } } 7, 62 -- BAYES: {0} } } 7, 62 -- SenderID: {0} } } 7, 62 -- DKIM: {0} } } 7, 62 -- TS: {0} } } 7, 62 -- SIG: {} } } 7, 62 -- DSC: {0} } } 7, 62 -- TRU_freehosting: {0} } } 7, 62 -- TRU_misc_spam: {0} } } 7, 62 -- TRU_marketing_spam: {0} } } 7, 62 -- TRU_profanity_spam: {0} } } 7, 62 -- TRU_spam1: {0} } } 7, 62 -- TRU_scam_spam: {0} } } 7, 62 -- TRU_money_spam: {0} } } 7, 62 -- URL Real-Time Signatures: {0} } } 7, 62 -- TRU_watch_spam: {0} } } 7, 62 -- TRU_embedded_image_spam: {1} } } 7, 62 -- TRU_spam2: {0} } } 7, 62 -- TRU_medical_spam: {0} } } 7, 62 -- TRU_playsites: {0} } } 7, 62 -- TRU_html_image_spam: {0} } } 7, 62 -- TRU_stock_spam: {0} } } 7, 62 -- TRU_urllinks: {0} } } 7, 62 -- TRU_adult_spam: {0} } } 7, 62 -- TRU_legal_spam: {0} } } 7, 62 -- TRU_lotto_spam: {0} } } 7, 62 -- TRU_ru_spamsubj: {0} } } 7, 62 -- TRU_phish_spam: {0} } } ==============================End of - Headers============================== } } } ==============================Original Headers============================== } 5, 39 -- From John.Mardinly-at-asu.edu Mon Oct 17 12:32:40 2011 } 5, 39 -- Received: from bcnet6.asu.edu (bcnet6.asu.edu [129.219.117.248]) } 5, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HHWe4A013994 } 5, 39 -- for {Microscopy-at-Microscopy.com} ; Mon, 17 Oct 2011 12:32:40 -0500 } 5, 39 -- X-ASG-Debug-ID: 1318872756-03efbe6e79fce090003-FOsErg } 5, 39 -- Received: from exhub1.asurite.ad.asu.edu (exhub1.asurite.ad.asu.edu [129.219.103.59]) by bcnet6.asu.edu with ESMTP id FSFmKdpGWqRjP8RZ (version=TLSv1 cipher=RC4-MD5 bits=128 verify=NO); Mon, 17 Oct 2011 10:32:37 -0700 (MST) } 5, 39 -- X-Barracuda-Envelope-From: John.Mardinly-at-asu.edu } 5, 39 -- X-Barracuda-Apparent-Source-IP: 129.219.103.59 } 5, 39 -- X-ASG-Whitelist: Client } 5, 39 -- Received: from EX01.asurite.ad.asu.edu (129.219.12.198) by } 5, 39 -- exhub1.asurite.ad.asu.edu (129.219.103.59) with Microsoft SMTP Server id } 5, 39 -- 8.2.255.0; Mon, 17 Oct 2011 10:32:36 -0700 } 5, 39 -- Received: from EX05.asurite.ad.asu.edu ([129.219.12.204]) by } 5, 39 -- EX01.asurite.ad.asu.edu with Microsoft SMTPSVC(6.0.3790.4675); Mon, 17 Oct } 5, 39 -- 2011 10:32:32 -0700 } 5, 39 -- Received: from 129.219.12.225 ([129.219.12.225]) by EX05.asurite.ad.asu.edu } 5, 39 -- ([129.219.12.225]) with Microsoft Exchange Server HTTP-DAV ; Mon, 17 Oct 2011 } 5, 39 -- 17:32:32 +0000 } 5, 39 -- Subject: [Filtered] Re: [Microscopy] Re: digital imaging standards } 5, 39 -- References: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} } 5, 39 -- X-ASG-Orig-Subj: Re: [Microscopy] Re: digital imaging standards } 5, 39 -- From: John Mardinly {John.Mardinly-at-asu.edu} } 5, 39 -- Content-Type: text/plain; charset="us-ascii" } 5, 39 -- In-Reply-To: {201110141738.p9EHcjKO017168-at-ns.microscopy.com} } 5, 39 -- Message-ID: {94BA1879-94B5-4C98-ADE4-0231231FCC76-at-exchange.asu.edu} } 5, 39 -- Thread-Topic: [Microscopy] Re: digital imaging standards } 5, 39 -- Thread-Index: AcyM8sBlFrqgOqJPS1KyooS3hOPGlQ== } 5, 39 -- Date: Mon, 17 Oct 2011 10:37:53 -0700 } 5, 39 -- To: {mcauliff-at-umdnj.edu} , MSA {Microscopy-at-Microscopy.com} } 5, 39 -- CC: {John.Mardinly-at-asu.edu} } 5, 39 -- MIME-Version: 1.0 (iPad Mail 8G4) } 5, 39 -- X-OriginalArrivalTime: 17 Oct 2011 17:32:32.0980 (UTC) FILETIME=[C0B28140:01CC8CF2] } 5, 39 -- X-Barracuda-Connect: exhub1.asurite.ad.asu.edu[129.219.103.59] } 5, 39 -- X-Barracuda-Start-Time: 1318872757 } 5, 39 -- X-Barracuda-Encrypted: RC4-MD5 } 5, 39 -- X-Barracuda-URL: http://129.219.117.248:8000/cgi-mod/mark.cgi } 5, 39 -- X-Virus-Scanned: by bsmtpd at asu.edu } 5, 39 -- Content-Transfer-Encoding: 8bit } 5, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HHWe4A013994 } ==============================End of - Headers============================== } }
-- =========================================== Dr. Nestor J. Zaluzec Argonne National Laboratory Electron Microscopy Center Materials Science/Bldg 212 Argonne, Illinois 60439 USA
Senior Scientist - Argonne National Laboratory Fellow of the Microscopy Society of America E.P. Wigner Fellow - Oak Ridge National Laboratory Senior Fellow of the Computational Institute - University of Chicago President Microscopy Society of America
===========================================
The box said ... "This program requires Win 95/98/NT/2K/XP/Vista or better..." So I bought a Mac !
===========================================
==============================Original Headers============================== 22, 27 -- From zaluzec-at-aaem.amc.anl.gov Mon Oct 17 13:01:18 2011 22, 27 -- Received: from znl.com ([206.69.208.20]) 22, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HI1I0W031018; 22, 27 -- Mon, 17 Oct 2011 13:01:18 -0500 22, 27 -- Received: from localhost (localhost [127.0.0.1]) 22, 27 -- by znl.com (Postfix) with ESMTP id 5F08C42A2EE; 22, 27 -- Mon, 17 Oct 2011 13:01:18 -0500 (CDT) 22, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 22, 27 -- Received: from znl.com ([127.0.0.1]) 22, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 22, 27 -- with ESMTP id IzVIzMiGExQr; Mon, 17 Oct 2011 13:01:17 -0500 (CDT) 22, 27 -- Received: from NestorPwrBk.local (unknown [187.126.150.126]) 22, 27 -- by znl.com (Postfix) with ESMTPA id B6F8B42A2E7; 22, 27 -- Mon, 17 Oct 2011 13:01:15 -0500 (CDT) 22, 27 -- Message-ID: {4E9C6D69.6030205-at-aaem.amc.anl.gov} 22, 27 -- Date: Mon, 17 Oct 2011 16:01:13 -0200 22, 27 -- From: "Nestor J. Zaluzec - ANL" {zaluzec-at-aaem.amc.anl.gov} 22, 27 -- Organization: ANL 22, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 22, 27 -- MIME-Version: 1.0 22, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 27 -- CC: Nestor_Zaluzec-at-microscopy.com 22, 27 -- Subject: digital imaging standards : JPEG vs Lossless 22, 27 -- References: {201110171732.p9HHWfF3014007-at-ns.microscopy.com} 22, 27 -- In-Reply-To: {201110171732.p9HHWfF3014007-at-ns.microscopy.com} 22, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 22, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Here is the official announcement of the Director position at our university:
John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
POSITION TITLE: Director, IMAGE (12 mo. term, A/P)
QUALIFICATIONS: Professional electron microscopist with a minimum of 4 years experience managing a centralized, service-oriented facility with multidisciplinary clients. Ph.D. in one of the hard sciences that includes a thorough understanding of theory and application of scanning and transmission electron microscopy, as well as ancillary techniques such as: energy dispersive X-ray microanalysis, ultramicrotomy, scanning transmission electron microscopy, vacuum coating procedures (sputtering, thermal evaporation) and specimen preparation techniques for biological and non-biological specimens. Experience must also include familiarity with light microscopy (conventional and fluorescence), image analysis and atomic force microscopy and grant writing. Preferred: Experience in problem solving (trouble-shooting) with advanced instrumentation. A working knowledge of graphics programs such as Photoshop, Illustrator, InDesign, Acrobat, PowerPoint for use in professional publications and large format posters. Able to work independently with minimal administrative oversight or support. Excellent organizational and communication skills (oral and written).
DUTIES: Oversee all operational aspects of the central IMAGE facility. This includes: (1) insuring performance of major instrumentation by training of technologists in basic maintenance (filament replacement, aperture cleaning, alignment) and clients in proper use of equipment, (2) trouble-shooting problems with equipment and determining best method to resolve issues, (3) developing long-range plan to replace aging equipment by pursuing external funding, (4) overseeing accounting (billing) and inventory control, (5) serving on student advisory committees and teaching courses in electron microscopy, (6) advising faculty with research projects involving electron microscopy, (7) training clients in proper methods to prepare posters for professional meetings, (8) overseeing printing of large-format posters and routine maintenance of poster printer, (9) providing tours of the facility to local colleges, schools and potential clients.
WEBSITE: www.orda.siu.edu/hr/image.html Additional information on the facility may be seen on our website.
APPLICATION DEADLINE: November 14, 2011, or until filled
TO APPLY: Send letter of application, current resume, names, addresses, and contact information for three references electronically via the website or hard copy to:
Chair, Search Committee Office of Sponsored Projects Administration SIU Carbondale 900 S Normal, Woody Hall C206, MC 4709 Carbondale, IL 62901
SIU Carbondale is an affirmative action/equal opportunity employer that strives to enhance its ability to develop a diverse faculty and staff and to increase its potential to serve a diverse student population. All applications are welcomed and encouraged and will receive consideration.
==============================Original Headers============================== 11, 18 -- From bozzola-at-siu.edu Mon Oct 17 16:21:50 2011 11, 18 -- Received: from mail-vw0-f41.google.com (mail-vw0-f41.google.com [209.85.212.41]) 11, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9HLLoxn003463 11, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 16:21:50 -0500 11, 18 -- Received: by vws16 with SMTP id 16so3059958vws.0 11, 18 -- for {Microscopy-at-microscopy.com} ; Mon, 17 Oct 2011 14:21:50 -0700 (PDT) 11, 18 -- MIME-Version: 1.0 11, 18 -- Received: by 10.52.28.141 with SMTP id b13mr22020535vdh.128.1318886510219; 11, 18 -- Mon, 17 Oct 2011 14:21:50 -0700 (PDT) 11, 18 -- Received: by 10.52.184.167 with HTTP; Mon, 17 Oct 2011 14:21:50 -0700 (PDT) 11, 18 -- Date: Mon, 17 Oct 2011 16:21:50 -0500 11, 18 -- Message-ID: {CAKeCJ9x=n6QFzwTijYTk-wkXm7gE-jWJCw7UzV2MhdtAXb=zDQ-at-mail.gmail.com} 11, 18 -- Subject: EM: Director Position Officially Announced 11, 18 -- From: John Bozzola {bozzola-at-siu.edu} 11, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 11, 18 -- Content-Type: text/plain; charset=UTF-8 11, 18 -- Content-Transfer-Encoding: 8bit 11, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9HLLoxn003463 ==============================End of - Headers==============================
I am reviewing the biopsy protocols used in our lab. One inconsistency I have come across is with regard to our cilia biopsies. I note that in our manual the cilia biopsies are supposed to be spit into two portions (at the hospital), one portion to be fixed in 1% tannic acid in 2% glutaraldehyde in 0.1M cacodylate buffer (freshly made up) and the other portion to be fixed in 2% glutaraldehyde in 0.1M cacodylate buffer (without tannic acid). After fixing for 1.5 hours the samples are then given 3 x buffer washes before being placed in the fridge for collection and further processing. I have noticed that recently this has not always been happening. Some nasal samples have simply been fixed in 2% glutaraldehyde in 0.1M cacodylate buffer before further processing. I have had no feedback from the pathologists about whether this is an issue or not. I have a couple of questions;
1. Do people doing TEM nasal biopsies still use tannic acid with the primary fixation ?
2. Is it still considered necessary?
3. If so, what protocol do you recommend?
Thanks for your time
Have a great day
Allan
==============================Original Headers============================== 11, 22 -- From allan.mitchell-at-stonebow.otago.ac.nz Tue Oct 18 16:00:07 2011 11, 22 -- Received: from mailhub2.otago.ac.nz (mailhub2.otago.ac.nz [139.80.64.247]) 11, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9IL05j8017493 11, 22 -- for {microscopy-at-microscopy.com} ; Tue, 18 Oct 2011 16:00:07 -0500 11, 22 -- Received: from anatomy.otago.ac.nz (anatomyy.otago.ac.nz [139.80.40.227]) 11, 22 -- by mailhub2.otago.ac.nz (8.13.8/8.13.8) with ESMTP id p9IL03m7009225 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Oct 2011 10:00:04 +1300 11, 22 -- Received: from ou040139.otago.ac.nz (ou040139.otago.ac.nz [139.80.40.139]) 11, 22 -- by anatomy.otago.ac.nz (Postfix) with ESMTP id 0D68850A6E5C 11, 22 -- for {microscopy-at-microscopy.com} ; Wed, 19 Oct 2011 10:00:04 +1300 (NZDT) 11, 22 -- Content-Type: text/plain; charset=us-ascii 11, 22 -- Mime-Version: 1.0 (Apple Message framework v1084) 11, 22 -- Subject: Re: Cilia Biopsies 11, 22 -- From: Allan Mitchell {allan.mitchell-at-stonebow.otago.ac.nz} 11, 22 -- In-Reply-To: {213626E590B5F9449C70B209CFC8AE4A016B6E41BA20-at-exchange2.adhb.govt.nz} 11, 22 -- Date: Wed, 19 Oct 2011 10:00:03 +1300 11, 22 -- Message-Id: {F005AF0D-32EB-44F4-8785-9587F059104C-at-stonebow.otago.ac.nz} 11, 22 -- References: {E5183191-80E4-4456-ABF7-14883A7CC13A-at-stonebow.otago.ac.nz} {213626E590B5F9449C70B209CFC8AE4A016B6E41BA1F-at-exchange2.adhb.govt.nz} {E53287BD-C778-4E61-A5D0-1C938E685F88-at-stonebow.otago.ac.nz} {213626E590B5F9449C70B209CFC8AE4A016B6E41BA20-at-exchange2.adhb.govt.nz} 11, 22 -- To: microscopy-at-microscopy.com 11, 22 -- X-Mailer: Apple Mail (2.1084) 11, 22 -- Content-Transfer-Encoding: 8bit 11, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9IL05j8017493 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jjm36-at-psu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jjm36-at-psu.edu Name: Joshua Maier
Organization: Penn State University
Title-Subject: [Filtered] EM Sample Preparation Charging
Message: Hello,
I work for Penn State University and oversee the Materials TEM Sample Preparation lab. Within the next month, the lab will be moved to a new building and combined with a general EM preparation lab. I was wondering how other institutes charge for preparing materials samples for TEM and SEM? Currently, we charge for ion mill time, my staff time and consumables. Our finances are being reviewed before this move and some adjustments need to be made. Please let me know what is working well.
After much effort we have been able to get our used Leo 906E TEM placed into its new home and have everything up and running.
As new users of TEM we are slowly working toward making the lab a working facility resource. I though it may be useful to identify and possibly make contact with other academic users of this specific TEM to exchange information that could help us.
If you own and run a Leo 906E, I and my novice operator would like to hear from you.
Thanks
Roy Beavers
Southern Methodist University Department of Earth Sciences P.O. Box 750395 Dallas, TX 75275 Voice: 214-768-2756 Fax: 214-768-2701 Email: rbeavers-at-smu.edu
==============================Original Headers============================== 9, 27 -- From rbeavers-at-mail.smu.edu Thu Oct 20 11:05:15 2011 9, 27 -- Received: from smtap1.systems.smu.edu (smtap1.systems.smu.edu [129.119.65.148]) 9, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9KG5EkH009680 9, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 11:05:15 -0500 9, 27 -- X-IronPort-Anti-Spam-Filtered: true 9, 27 -- X-IronPort-Anti-Spam-Result: Av0EALZFoE6Bd0GG/2dsb2JhbAA5BwOpGIEFgXAFOlEBKhQsFiYBBBMInjiWX4kChHoagjRhBIZBnxY 9, 27 -- Received: from sxht1p3.systems.smu.edu ([129.119.65.134]) 9, 27 -- by smtah1.systems.smu.edu with ESMTP/TLS/AES128-SHA; 20 Oct 2011 11:05:14 -0500 9, 27 -- Received: from SXMB1PG.SYSTEMS.SMU.EDU ([fe80::54f4:609f:2958:17b2]) by 9, 27 -- SXHT1P3.SYSTEMS.SMU.EDU ([2002:8177:4186::8177:4186]) with mapi id 9, 27 -- 14.01.0289.001; Thu, 20 Oct 2011 11:05:13 -0500 9, 27 -- From: "Beavers, Roy" {rbeavers-at-mail.smu.edu} 9, 27 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 9, 27 -- Subject: Locating Academic Labs who run Leo 906E TEM 9, 27 -- Thread-Topic: Locating Academic Labs who run Leo 906E TEM 9, 27 -- Thread-Index: AcyPQKdIdhIxtOTKQhOAdAsEo/ba5w== 9, 27 -- Date: Thu, 20 Oct 2011 16:05:11 +0000 9, 27 -- Message-ID: {BC0C36E74D341741A25CE2ABB122E7A32A30BB4F-at-SXMB1PG.SYSTEMS.SMU.EDU} 9, 27 -- Accept-Language: en-US 9, 27 -- Content-Language: en-US 9, 27 -- X-MS-Has-Attach: 9, 27 -- X-MS-TNEF-Correlator: 9, 27 -- x-originating-ip: [129.119.70.150] 9, 27 -- Content-Type: text/plain; charset="us-ascii" 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9KG5EkH009680 ==============================End of - Headers==============================
This is a summary of the replies I got to my question about resin disposal.
First, let me apologize for not clearly stating that we do not really dispose of resin down the sink. Never did, never will. It was a bad joke.
There were two main suggestions for resin disposal. Leave it in the hood to let the solvent evaporate, then into the oven for polymerization and out in the trash, or mix all the infiltration steps to increase the concentration of plastic so it will polymerize and then out, or both.
There were some other specific recommendations that were insightful to me. One was to OD the plastic with catalyst to speedup polymerization of reluctant mixtures. There’s always too much catalyst in those kits anyway and it would be good to move it along.
Another suggestion was to use the empty bottles that the resin components come in as waste bottles for mixtures to be discarded.
As always, there are red herrings that show up in situations like this. At a previous institution we were advised against letting solvents evaporate in the hood as a means of disposal. Among the reasons were local air quality regulations that had to account for how much solvent we were adding to the atmosphere and EH&S wanted that minimized.
It can also be problematic in determining the proper category of ‘waste’ for resin. At the same former institution, we had strict rules about calling anything a ‘waste’. Once a container is labeled as ‘waste’ we had to move it along within 6 months, no keeping ‘waste’ containers in the hood and no letting it build up over time. On the other hand, if it is not labeled as ‘waste’ it could stay around for a long time. So if liquid resin is toxic and considered a waste and polymerized resin is not toxic and may be tossed in the regular trash, seems to be a little Catch-22 going on.
Several replies suggested working with our hazardous waste office. That’s a great idea, except that the hazardous waste guys here think Osmium is some kind of male skin treatment (go ahead, http://www.osmiumformen.com/).
The best reply was from a lab that keeps a 5 gal can of acetone next to the sink to wash the resin down the pipes. Only problem they reported was the need to replace the plastic pipes fairly often.
All in all, there were great replies that helped me a lot.
I haven’t had such a good time since I tried to read the vacuum gauge on my VE.
Now I need to figure out what to do with the GD UA.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 20, 34 -- From jkrupp-at-deltacollege.edu Thu Oct 20 12:30:06 2011 20, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 20, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9KHU64m012340 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 12:30:06 -0500 20, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 20, 34 -- by localhost (Email Security Appliance) with SMTP id C505914FE3_EA05A9DB 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 17:30:05 +0000 (GMT) 20, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 20, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 6BB37150AD_EA05A9DF 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 17:30:05 +0000 (GMT) 20, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 20, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 20, 34 -- ESMTP id LTDKM400.PTU for {Microscopy-at-microscopy.com} ; Thu, 20 20, 34 -- Oct 2011 10:30:04 -0700 20, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 20, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 3630512B345E3 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 10:30:05 -0700 (PDT) 20, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 20, 34 -- with ESMTP id SDx1oNmWHqBJ for {Microscopy-at-microscopy.com} ; 20, 34 -- Thu, 20 Oct 2011 10:30:01 -0700 (PDT) 20, 34 -- Received: from [172.20.2.8] (unknown [172.20.2.8]) 20, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 3119D12B345B4 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 10:30:01 -0700 (PDT) 20, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 20, 34 -- Content-Type: text/plain; charset=windows-1252 20, 34 -- Subject: Resin disposal replies 20, 34 -- Date: Thu, 20 Oct 2011 10:30:00 -0700 20, 34 -- Message-Id: {EDC71135-8FBB-40CC-ACF0-E113A9164343-at-deltacollege.edu} 20, 34 -- To: Microscopy-at-microscopy.com 20, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 20, 34 -- X-Mailer: Apple Mail (2.1084) 20, 34 -- Content-Transfer-Encoding: 8bit 20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9KHU64m012340 ==============================End of - Headers==============================
-----Original Message----- X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu] Sent: Thursday, October 20, 2011 1:38 PM To: Frank Karl
Hi again:
This is a summary of the replies I got to my question about resin disposal.
First, let me apologize for not clearly stating that we do not really dispose of resin down the sink. Never did, never will. It was a bad joke.
There were two main suggestions for resin disposal. Leave it in the hood to let the solvent evaporate, then into the oven for polymerization and out in the trash, or mix all the infiltration steps to increase the concentration of plastic so it will polymerize and then out, or both.
There were some other specific recommendations that were insightful to me. One was to OD the plastic with catalyst to speedup polymerization of reluctant mixtures. There’s always too much catalyst in those kits anyway and it would be good to move it along.
Another suggestion was to use the empty bottles that the resin components come in as waste bottles for mixtures to be discarded.
As always, there are red herrings that show up in situations like this. At a previous institution we were advised against letting solvents evaporate in the hood as a means of disposal. Among the reasons were local air quality regulations that had to account for how much solvent we were adding to the atmosphere and EH&S wanted that minimized.
It can also be problematic in determining the proper category of ‘waste’ for resin. At the same former institution, we had strict rules about calling anything a ‘waste’. Once a container is labeled as ‘waste’ we had to move it along within 6 months, no keeping ‘waste’ containers in the hood and no letting it build up over time. On the other hand, if it is not labeled as ‘waste’ it could stay around for a long time. So if liquid resin is toxic and considered a waste and polymerized resin is not toxic and may be tossed in the regular trash, seems to be a little Catch-22 going on.
Several replies suggested working with our hazardous waste office. That’s a great idea, except that the hazardous waste guys here think Osmium is some kind of male skin treatment (go ahead, http://www.osmiumformen.com/).
The best reply was from a lab that keeps a 5 gal can of acetone next to the sink to wash the resin down the pipes. Only problem they reported was the need to replace the plastic pipes fairly often.
All in all, there were great replies that helped me a lot.
I haven’t had such a good time since I tried to read the vacuum gauge on my VE.
Now I need to figure out what to do with the GD UA.
Jon
Jonathan Krupp Delta College 5151Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
==============================Original Headers============================== 20, 34 -- From jkrupp-at-deltacollege.edu Thu Oct 20 12:30:06 2011 20, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 20, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9KHU64m012340 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 12:30:06 -0500 20, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 20, 34 -- by localhost (Email Security Appliance) with SMTP id C505914FE3_EA05A9DB 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 17:30:05 +0000 (GMT) 20, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 20, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id 6BB37150AD_EA05A9DF 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 17:30:05 +0000 (GMT) 20, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 20, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 20, 34 -- ESMTP id LTDKM400.PTU for {Microscopy-at-microscopy.com} ; Thu, 20 20, 34 -- Oct 2011 10:30:04 -0700 20, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 20, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 3630512B345E3 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 10:30:05 -0700 (PDT) 20, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 20, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 20, 34 -- with ESMTP id SDx1oNmWHqBJ for {Microscopy-at-microscopy.com} ; 20, 34 -- Thu, 20 Oct 2011 10:30:01 -0700 (PDT) 20, 34 -- Received: from [172.20.2.8] (unknown [172.20.2.8]) 20, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 3119D12B345B4 20, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 10:30:01 -0700 (PDT) 20, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 20, 34 -- Content-Type: text/plain; charset=windows-1252 20, 34 -- Subject: Resin disposal replies 20, 34 -- Date: Thu, 20 Oct 2011 10:30:00 -0700 20, 34 -- Message-Id: {EDC71135-8FBB-40CC-ACF0-E113A9164343-at-deltacollege.edu} 20, 34 -- To: Microscopy-at-microscopy.com 20, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 20, 34 -- X-Mailer: Apple Mail (2.1084) 20, 34 -- Content-Transfer-Encoding: 8bit 20, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9KHU64m012340 ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 32, 28 -- From frank_karl-at-ardl.com Thu Oct 20 13:38:34 2011 32, 28 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 32, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9KIcXgE029924 32, 28 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 13:38:34 -0500 32, 28 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 32, 28 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 20 32, 28 -- Oct 2011 14:38:26 -0400 32, 28 -- From: Frank Karl {frank_karl-at-ardl.com} 32, 28 -- To: "'jkrupp-at-deltacollege.edu'" {jkrupp-at-deltacollege.edu} , 32, 28 -- "Microscopy 32, 28 -- Listserver (E-mail)" {microscopy-at-microscopy.com} 32, 28 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 32, 28 -- Return-Receipt-To: {frank_karl-at-ardl.com} 32, 28 -- Date: Thu, 20 Oct 2011 14:38:25 -0400 32, 28 -- Subject: was Resin disposal replies now osmium for men 32, 28 -- Thread-Topic: was Resin disposal replies now osmium for men 32, 28 -- Thread-Index: AcyPTwhdLQ5MX0fcSemhax8hfY1YpQAB5n4w 32, 28 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF150770103D0DFF4-at-exchange2k7.ad.ardl.com} 32, 28 -- In-Reply-To: {201110201738.p9KHc00C027853-at-ns.microscopy.com} 32, 28 -- Accept-Language: en-US 32, 28 -- Content-Language: en-US 32, 28 -- X-MS-Has-Attach: 32, 28 -- X-MS-TNEF-Correlator: 32, 28 -- acceptlanguage: en-US 32, 28 -- Content-Type: text/plain; charset="Windows-1252" 32, 28 -- MIME-Version: 1.0 32, 28 -- Content-Transfer-Encoding: 8bit 32, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9KIcXgE029924 ==============================End of - Headers==============================
Dear List Hunter College is looking for an individual to teach a one semester electron microscopy course in the Spring of 2012. This school is located on the upper east side of New York City, at 68th street and Lexington. The class would meet twice a week for lectures and lab. The class meeting are currently scheduled for Tuesday and Wednesday afternoon. It would be at the introductory level covering the basic theoretical background of TEM, sample preparation and imaging. The position would be at the level of an adjunct faculty appointment. Interested candidates should email their resume to microscopy-at-genectr.hunter.cuny.edu
Thank you
Dr. Lloyd Williams Dept. of Biology Hunter College 695 Park Ave New York NY 10065
==============================Original Headers============================== 4, 23 -- From Williams-at-GENECTR.HUNTER.CUNY.EDU Thu Oct 20 13:56:56 2011 4, 23 -- Received: from GeneMX.hunter.cuny.edu (genemx.hunter.cuny.edu [146.95.150.49]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9KIusXX014345 4, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 13:56:55 -0500 4, 23 -- Received: from GeneExchange.bio.hunter.cuny.edu ([169.254.2.140]) by 4, 23 -- genemx.bio.hunter.cuny.edu ([fe80::9481:a04e:9f1c:f509%9]) with mapi id 4, 23 -- 14.01.0339.001; Thu, 20 Oct 2011 14:58:43 -0400 4, 23 -- From: Lloyd Williams {Williams-at-GENECTR.HUNTER.CUNY.EDU} 4, 23 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 4, 23 -- Subject: Opening To Teach TEM Course at Hunter College 4, 23 -- Thread-Topic: Opening To Teach TEM Course at Hunter College 4, 23 -- Thread-Index: AcyPWkI0fKEhj1b5RqeHkVTHfrzOXw== 4, 23 -- Date: Thu, 20 Oct 2011 18:58:42 +0000 4, 23 -- Message-ID: {82442657AE7F624586F6FCBFF78E804026162C5E-at-GeneExchange.bio.hunter.cuny.edu} 4, 23 -- Accept-Language: en-US 4, 23 -- Content-Language: en-US 4, 23 -- X-MS-Has-Attach: 4, 23 -- X-MS-TNEF-Correlator: 4, 23 -- x-originating-ip: [146.95.148.210] 4, 23 -- Content-Type: text/plain; charset="us-ascii" 4, 23 -- MIME-Version: 1.0 4, 23 -- Content-Transfer-Encoding: 8bit 4, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9KIusXX014345 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both lamiller-at-illinois.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: lamiller-at-illinois.edu Name: Lou Ann Miller
Organization: University of Illinois
Title-Subject: [Filtered] Fixing a Malvern zetasier
Message: Greetings,
While not directly related to microscopes, our facility has a zetasizer.
I was wondering if any of your facilities do too, and if you do have a Malvern zetasizer, do you know of anyone besides Malvern that fixes them?
Thanks!
Lou Ann ( I'm on the list, so if you reply to the list I will see it) Login Host: 130.126.102.8 ---------------------------------------------------------------------------
==============================Original Headers============================== 13, 25 -- From microscopylistserver-noreply-at-microscopy.com Thu Oct 20 23:17:04 2011 13, 25 -- Received: from znl.com ([206.69.208.20]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9L4H4sw006370 13, 25 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 23:17:04 -0500 13, 25 -- Received: from localhost (localhost [127.0.0.1]) 13, 25 -- by znl.com (Postfix) with ESMTP id 732BA42CDB5 13, 25 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 23:17:04 -0500 (CDT) 13, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 13, 25 -- Received: from znl.com ([127.0.0.1]) 13, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 13, 25 -- with ESMTP id eKUL9PjT3zFn for {microscopy-at-microscopy.com} ; 13, 25 -- Thu, 20 Oct 2011 23:17:03 -0500 (CDT) 13, 25 -- Received: from NestorPwrBk.local (96-36-123-218.static.hckr.nc.charter.com [96.36.123.218]) 13, 25 -- by znl.com (Postfix) with ESMTPA id 59BB242CDAA 13, 25 -- for {microscopy-at-microscopy.com} ; Thu, 20 Oct 2011 23:17:03 -0500 (CDT) 13, 25 -- Message-ID: {4EA0F23E.30702-at-microscopy.com} 13, 25 -- Date: Thu, 20 Oct 2011 23:17:02 -0500 13, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 13, 25 -- Reply-To: lamiller-at-illinois.edu 13, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 13, 25 -- MIME-Version: 1.0 13, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 13, 25 -- Subject: viaWWW:Fixing a Malvern zetasier 13, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 13, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
What level of students are you working with? I have a long list of things that make good samples for education that you don't have to order- they can be readily found around your house or school.
For example, when I taught high school, I had a couple of students work on a project with the SEM that involved comparing the crystal morphology between real sugar and several brands of artificial sweetener. They also compared other properties like solubility, melting point, etc.
The main issue you will find is that many specimens need certain types of preparation. If you don't have access to a CPD or other dehydration system, you might find it difficult to image something like seaweed or other wet materials.
It also depends on the capabilities of your scope- if you have EDS, than something simple like a rock or a piece of welded steel might make a good sample- examining the chemical differences between materials that look very similar. BS and EDS make excellent pictures using some types of rocks. (I have a beautiful copper ore sample I'd be happy to send you a hunk of- just send me your address. It looks great under BS, and even better under EDS.)
Another really good specimen is an old wind-up watch. Take the mechanism out and wind it up, then use the SEM to show the movement of the hour hand.
--Justin A. Kraft Applications Engineer IXRF Systems
On Oct 21, 2011, at 10:01 AM, jafarhan-at-rci.rutgers.edu wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear listers, } } I am looking to purchase educational SEM samples for imaging, EDS, BS, and } EBSD. I wonder if there is any single source. Any advice is appreciated. } } Thanks, } } Jafar } } } ==============================Original Headers============================== } 5, 32 -- From jafarhan-at-rci.rutgers.edu Fri Oct 21 09:56:37 2011 } 5, 32 -- Received: from annwn41.rutgers.edu (whitelist-out.rutgers.edu [128.6.68.238]) } 5, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9LEubnp000986 } 5, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 09:56:37 -0500 } 5, 32 -- Received: from localhost (localhost [127.0.0.1]) } 5, 32 -- by annwn41.rutgers.edu (Postfix) with ESMTP id CAF3D74802B } 5, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 10:56:36 -0400 (EDT) } 5, 32 -- X-Virus-Scanned: Virus Scanned by NBCS } 5, 32 -- Received: from annwn41.rutgers.edu ([127.0.0.1]) } 5, 32 -- by localhost (annwn41.rutgers.edu [127.0.0.1]) (amavisd-new, port 10024) } 5, 32 -- with ESMTP id a9lfdOkEpwEk for {Microscopy-at-microscopy.com} ; } 5, 32 -- Fri, 21 Oct 2011 10:56:36 -0400 (EDT) } 5, 32 -- Received: from rci.rutgers.edu (unknown [192.168.160.201]) } 5, 32 -- by annwn41.rutgers.edu (Postfix) with ESMTP id AD179748029 } 5, 32 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 10:56:36 -0400 (EDT) } 5, 32 -- Received: by rci.rutgers.edu (Postfix, from userid 11335) } 5, 32 -- id 9653E1256; Fri, 21 Oct 2011 10:56:36 -0400 (EDT) } 5, 32 -- Received: from 128.6.22.140 } 5, 32 -- (SquirrelMail authenticated user jafarhan) } 5, 32 -- by webmail.rci.rutgers.edu with HTTP; } 5, 32 -- Fri, 21 Oct 2011 10:56:36 -0400 (EDT) } 5, 32 -- Message-ID: {a480e11edcef6e28314c9191b9c85d73.squirrel-at-webmail.rci.rutgers.edu} } 5, 32 -- Date: Fri, 21 Oct 2011 10:56:36 -0400 (EDT) } 5, 32 -- Subject: SEM educational samples } 5, 32 -- From: jafarhan-at-rci.rutgers.edu } 5, 32 -- To: Microscopy-at-microscopy.com } 5, 32 -- User-Agent: SquirrelMail/1.4.16 } 5, 32 -- MIME-Version: 1.0 } 5, 32 -- Content-Type: text/plain;charset=iso-8859-1 } 5, 32 -- Content-Transfer-Encoding: 8bit } 5, 32 -- X-Priority: 3 (Normal) } 5, 32 -- Importance: Normal } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 33 -- From kraftpiano-at-gmail.com Fri Oct 21 10:35:07 2011 10, 33 -- Received: from mail-gy0-f169.google.com (mail-gy0-f169.google.com [209.85.160.169]) 10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9LFZ77o018246 10, 33 -- for {microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 10:35:07 -0500 10, 33 -- Received: by gyf3 with SMTP id 3so4724135gyf.0 10, 33 -- for {microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 08:35:07 -0700 (PDT) 10, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 33 -- d=gmail.com; s=gamma; 10, 33 -- h=subject:mime-version:content-type:from:in-reply-to:date:cc 10, 33 -- :content-transfer-encoding:message-id:references:to:x-mailer; 10, 33 -- bh=zFRR/mWco0MbHP4wKroA6F9RcmYd+453bZ7BGxKRKAY=; 10, 33 -- b=Fyge53o2ccB6I+ZDb2WuZ10YeHj6UfTFkvCa7Xjq84J3tGMmbydK9OSkqrXtntWypc 10, 33 -- 57MbY+w9vUjStWcEnAIG9eib3o/h8gLEkHvWCWZKJ8uWnlOvFeZj8b1awEdomYB3IKu/ 10, 33 -- YXnQlLKgGnF6H/Dj+Fs93PqKPaDoHxHiGedSM= 10, 33 -- Received: by 10.146.7.13 with SMTP id 13mr3424059yag.15.1319211305275; 10, 33 -- Fri, 21 Oct 2011 08:35:05 -0700 (PDT) 10, 33 -- Received: from [192.168.15.106] (c-98-195-153-235.hsd1.tx.comcast.net. [98.195.153.235]) 10, 33 -- by mx.google.com with ESMTPS id j26sm36608930ann.7.2011.10.21.08.35.02 10, 33 -- (version=TLSv1/SSLv3 cipher=OTHER); 10, 33 -- Fri, 21 Oct 2011 08:35:03 -0700 (PDT) 10, 33 -- Subject: Re: [Microscopy] SEM educational samples 10, 33 -- Mime-Version: 1.0 (Apple Message framework v1244.3) 10, 33 -- Content-Type: text/plain; charset=us-ascii 10, 33 -- From: Justin Kraft {kraftpiano-at-gmail.com} 10, 33 -- In-Reply-To: {201110211501.p9LF1sqN008638-at-ns.microscopy.com} 10, 33 -- Date: Fri, 21 Oct 2011 10:35:05 -0500 10, 33 -- Cc: microscopy-at-microscopy.com 10, 33 -- Message-Id: {DB76E1F3-CF69-435B-A43D-33BC0B8376D8-at-gmail.com} 10, 33 -- References: {201110211501.p9LF1sqN008638-at-ns.microscopy.com} 10, 33 -- To: jafarhan-at-rci.rutgers.edu 10, 33 -- X-Mailer: Apple Mail (2.1244.3) 10, 33 -- Content-Transfer-Encoding: 8bit 10, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9LFZ77o018246 ==============================End of - Headers==============================
A colleague is interested in localizing 5-HT-1B antigen in synapses of CNS tissues. He wants to use a pre-embedding labeling method. However, I suggested he consider using post-embedding staining, as follows. 1. Perfusion fixation of CNS specimen (formaldehyde/glutaraldehyde), 2. Quenching of residual fixative using lysine, 3. Embedding in LR White, 4. Reaction of sections with primary antibody followed by gold-labeled secondary antibody.
Questions: 1. Is there a reason for using pre-embedding labeling with this system? 2. Any recommended fixatives for optimum preservation of the 5-HT-1B antigen? 3. Any recommended protocols?
Thank you.
-- John J. Bozzola, Ph.D., Professor & Director of IMAGE Integrated Microscopy & Graphics Expertise Southern Illinois University 750 Communications Drive Carbondale, ILÂ 62901 Phone: 618-453-3730
==============================Original Headers============================== 5, 18 -- From bozzola-at-siu.edu Fri Oct 21 14:18:58 2011 5, 18 -- Received: from mail-vx0-f169.google.com (mail-vx0-f169.google.com [209.85.220.169]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9LJIwCn005727 5, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 14:18:58 -0500 5, 18 -- Received: by vcbfk1 with SMTP id fk1so4779313vcb.0 5, 18 -- for {Microscopy-at-microscopy.com} ; Fri, 21 Oct 2011 12:18:57 -0700 (PDT) 5, 18 -- MIME-Version: 1.0 5, 18 -- Received: by 10.52.30.79 with SMTP id q15mr15281937vdh.111.1319224737656; Fri, 5, 18 -- 21 Oct 2011 12:18:57 -0700 (PDT) 5, 18 -- Received: by 10.52.184.167 with HTTP; Fri, 21 Oct 2011 12:18:57 -0700 (PDT) 5, 18 -- Date: Fri, 21 Oct 2011 14:18:57 -0500 5, 18 -- Message-ID: {CAKeCJ9zLvkLwxbR6kJeZ7taymspQPSkB8US0UNY9qMKXFWieTA-at-mail.gmail.com} 5, 18 -- Subject: EM: Immune Labeling of Neurological Tissues 5, 18 -- From: John Bozzola {bozzola-at-siu.edu} 5, 18 -- To: MSAListserver {Microscopy-at-microscopy.com} 5, 18 -- Content-Type: text/plain; charset=UTF-8 5, 18 -- Content-Transfer-Encoding: 8bit 5, 18 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9LJIwCn005727 ==============================End of - Headers==============================
5-HT-1B is a serotonin (5-hydroxytrptamine) receptor subtype. In rat CNS, 5-HT-1B has been reported to be intensely present in the substantia nigra and globus pallidus of brain. At the ultrastructure level, 5-HT-1B has been shown to be associated with the plasma membrane of preterminal axons.
Immunolabeling in brain tissue is commonly done on 40-50 µm vibrating microtome sections using the pre-embedding immunoperoxidase or immunogold (with ultrasmall gold conjugates) methods. These methods allow researchers to evaluate the distribution of the labeling over the large and anatomically heterogeneous brain regions at LM level. The structure of interest can then be identified on flat embedded vibrating microtome sections and re-embedded/thin sectioned for EM analysis.
Another advantage that the pre-embedding method offers is its relatively good preservation and contrast of membrane structure. For localization analysis at the ultrastructual level, the membrane integrity of brain tissue is particularly critical. This is because 1. The membrane is needed for the identification of different neuronal profiles, and 2. The localization interests are often membrane proteins (receptors, transporters, channel proteinss). In my experience, the structural preservation obtained by embedding in LR White resin is not adequate for brain immuno labeling. Since osmium tetroxide is not usually used in LR White embedding protocol, the lipid bilayer can be severely extracted during the dehydration or "melted" during the subsequent polymerization in oven (50 degree C).
For the preservation of antigenicity, the pre-embedding method is also more suitable compared to the resin embedding as the only treatment in pre-embedding labeling that could potentially alter the antigen structure is the initial fixation.
Nevertheless, post-embedding method has been used to answer specific localization questions in brain tissue. In these cases, the low temperature embedding method (high pressure freezing, cryo-substitution, and Lowicryl embedding) were usually preferred, and sometimes, cryo-ultramicrotomy.
The type of fixative to use depends on the "tolerance" of the epitope(s) that a particular antibody binds to. The antibody vendors often provide the information on suitable fixation. Information can also be found in literature. However, one does need to pay attention to the source of antibody in literature search. Antibodies can be made to bind different epitopes of the same protein. A suitable fixative for labeling using a particular antibody does not guarantee the same positive result when a different antibody is used even if both are against the same protein.
Hope this helps. Feel free to contact me off-line more detail is needed. Thank you.
Hong
On 10/21/11 3:22 PM, "bozzola-at-siu.edu" {bozzola-at-siu.edu} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
________________________________
This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.
If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
==============================Original Headers============================== 22, 39 -- From hyi-at-emory.edu Mon Oct 24 10:10:09 2011 22, 39 -- Received: from ndb-mr1.cc.emory.edu (ndb-mr1.cc.emory.edu [170.140.52.40]) 22, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9OFA9aO031687 22, 39 -- for {Microscopy-at-microscopy.com} ; Mon, 24 Oct 2011 10:10:09 -0500 22, 39 -- Received: from e14edge2w.Eu.Emory.Edu (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 22, 39 -- by ndb-mr1.cc.emory.edu (8.13.8/8.13.1) with ESMTP id p9OFA6V8022156; 22, 39 -- Mon, 24 Oct 2011 11:10:06 -0400 22, 39 -- Received: from E14CH1N.Enterprise.emory.net (10.240.8.113) by 22, 39 -- e14edge2w.Eu.Emory.Edu (170.140.108.22) with Microsoft SMTP Server (TLS) id 22, 39 -- 14.1.339.1; Mon, 24 Oct 2011 11:10:06 -0400 22, 39 -- Received: from E14MBX15N.Enterprise.emory.net ([fe80::fcd7:9c7d:7350:3f64]) by 22, 39 -- e14ch1n.Enterprise.emory.net ([::1]) with mapi id 14.01.0339.001; Mon, 24 Oct 22, 39 -- 2011 11:10:05 -0400 22, 39 -- From: "Yi, Hong" {hyi-at-emory.edu} 22, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 22, 39 -- CC: "bozzola-at-siu.edu" {bozzola-at-siu.edu} 22, 39 -- Subject: Re: [Microscopy] EM: Immune Labeling of Neurological Tissues 22, 39 -- Thread-Topic: [Microscopy] EM: Immune Labeling of Neurological Tissues 22, 39 -- Thread-Index: AQHMkl8CCHJ02b2IQUmNP9Wq7+ei7Q== 22, 39 -- Date: Mon, 24 Oct 2011 15:10:05 +0000 22, 39 -- Message-ID: {CAC988D6.FC85%hyi-at-emory.edu} 22, 39 -- Accept-Language: en-US 22, 39 -- Content-Language: en-US 22, 39 -- X-MS-Has-Attach: 22, 39 -- X-MS-TNEF-Correlator: 22, 39 -- user-agent: Microsoft-MacOutlook/14.12.0.110505 22, 39 -- x-originating-ip: [170.140.30.131] 22, 39 -- Content-Type: text/plain; charset="iso-8859-1" 22, 39 -- Content-ID: {6B7BD210D1C73A48831959E29196CF59-at-Enterprise.emory.net} 22, 39 -- MIME-Version: 1.0 22, 39 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 22, 39 -- X-Emory-MailScanner-ID: p9OFA6V8022156 22, 39 -- X-Emory-MailScanner: Found to be clean 22, 39 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 22, 39 -- score=-0.504, required 8, autolearn=disabled, RP_MATCHES_RCVD -0.50) 22, 39 -- X-Emory-MailScanner-From: hyi-at-emory.edu 22, 39 -- X-Spam-Status: No 22, 39 -- Content-Transfer-Encoding: 8bit 22, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9OFA9aO031687 ==============================End of - Headers==============================
When I read about thowing the resin in the sink (can't even imagine imagining it), it reminds me of a special "freaky" moment during my PhD thesis. I was working on HTLV-I (human T-cell leukemia virus type I), the other human retrovirus and as kind to human beings as HIV is. Especially, we were growing cells containing the provirus in culture (the cells have integrated the viral genome in their own genome). Needless to say that we were very far from biosafety level 2 conditions back then. As usual in cell culture procedure, the medium was regularly changed, which involved throwing the old medium in the sink and giving fresh medium to the cells. Of course the first time I was asked to do that I asked my supervisor if we were not disseminating viral particles in the environment and she replied that the cells were producing "minimal amounts" of virus and that anyway the particles will be extremely diluted in the environment and that a minimal virus concentration was necessary to effectively infect. As naive as a student can be, I believed this experienced researcher knew what we were doing. It is only years later that I read about the cells producing more-than-minimal amounts of virus! Kind of scientific terrorism, isn't it?
Stephane
==============================Original Headers============================== 2, 36 -- From nizets2-at-yahoo.com Tue Oct 25 02:57:00 2011 2, 36 -- Received: from nm25-vm0.bullet.mail.sp2.yahoo.com (nm25-vm0.bullet.mail.sp2.yahoo.com [98.139.91.228]) 2, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9P7v0L1030194 2, 36 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 02:57:00 -0500 2, 36 -- Received: from [98.139.91.70] by nm25.bullet.mail.sp2.yahoo.com with NNFMP; 25 Oct 2011 07:56:59 -0000 2, 36 -- Received: from [98.139.91.31] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 25 Oct 2011 07:56:59 -0000 2, 36 -- Received: from [127.0.0.1] by omp1031.mail.sp2.yahoo.com with NNFMP; 25 Oct 2011 07:56:59 -0000 2, 36 -- X-Yahoo-Newman-Property: ymail-3 2, 36 -- X-Yahoo-Newman-Id: 593727.19365.bm-at-omp1031.mail.sp2.yahoo.com 2, 36 -- Received: (qmail 9766 invoked by uid 60001); 25 Oct 2011 07:56:59 -0000 2, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1319529419; bh=/MEpyOZaAhE8oXZqlrf3TfwUgGNtpnuNcpo68fUksB0=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=0lm9sHWTQ9iuVL08QJ/EiGIHFh3/lo6PUlX3sI6erRnxMeGIwvG2T3alrQQz0Vzg9FrgIZHtjqGYRtGeglsUY6AQkOIKestUr1vPEN4IJe53Ly9UxXhhPFR1ONAQyVR0r/5ypc7ZhWEopshOnueLo2VeMg5fiCYfPlLe1/HAJk0= 2, 36 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 36 -- s=s1024; d=yahoo.com; 2, 36 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 2, 36 -- b=gUn24GUBBhnvagZgzOisGx+VRlLq1r7qiHYT82NKuqhA7z/QKo1T3njo1Qiy7KgliVRZNs7x9RTOY21fK0DzRL9AkR2i3LEUVkY0pbkFrG8ZNV+BlmSBt8HIxc0RFnRgXnNTt5F12zEcHkIPQIcYGm5Pif14MvlAPxtoAOGTqUo=; 2, 36 -- X-YMail-OSG: ZoCNyYsVM1lS5_zWAJOxekRrVcSMjd6y9_usCQZ3PoMo.R. 2, 36 -- 1E4GeJAA4M_cqybHlAlCNoTvxInlaauHDAXSLzeqBlIPyzigK9eLmZgVZabt 2, 36 -- aFsD2WoZEQkX3slZ3ZlEDBWhcc9k33tuHVTuhSlPXw7DawTaTwDH22RRP58d 2, 36 -- aXYb44ApeYk55x.mflpcoh5_t.CvHtLgqPRiOcqACDA81SeO.PJLKHU4Q33g 2, 36 -- .pGusMUW8d6XlUATlAvnKowPgyzB.Y432idsn6fhxgnf2pDaMbUONDhzJzYT 2, 36 -- LPMZXl.MALYpFifsl3hWVkWrKpjDRrntltR3cA07r6nCIvNrol5MqnbLGLD5 2, 36 -- uJesyKi7Kj.42Vf_040n.XOw4sWtCoKDf7xi9UPCCvHYD8VIe25jX..p8DP6 2, 36 -- tMnSvf_YXm2le1wbhrfZwNtfnE27C8cgI.Hf12e5.8DnWYt7ng_y9AL6kBE6 2, 36 -- krg-- 2, 36 -- Received: from [80.122.101.100] by web110809.mail.gq1.yahoo.com via HTTP; Tue, 25 Oct 2011 00:56:59 PDT 2, 36 -- X-Mailer: YahooMailWebService/0.8.114.317681 2, 36 -- Message-ID: {1319529419.4698.YahooMailNeo-at-web110809.mail.gq1.yahoo.com} 2, 36 -- Date: Tue, 25 Oct 2011 00:56:59 -0700 (PDT) 2, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} 2, 36 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 2, 36 -- Subject: down the sink 2, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 36 -- MIME-Version: 1.0 2, 36 -- Content-Type: text/plain; charset=iso-8859-1 2, 36 -- Content-Transfer-Encoding: 8bit 2, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9P7v0L1030194 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both corrie.van-hoek-at-tatasteel.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: corrie.van-hoek-at-tatasteel.com Name: Corrie van Heok
Organization: Tata Steel Europe
Title-Subject: [Filtered] Peak position, shape and resolution deterioration using Silicon Drift Detectors
Message: We use Thermo SDD detectors mounted on a JEOL-7001F microscope for rapid collection of X-rays for Spectral Imaging, Inclusion analysis and light element analysis. A first detector set was showing, within a year, a peak shift, a strong tailing on the left side of peak and a decrease in peak resolution in the low energy range (0-1keV) and has therefore replaced. The 2nd set of SDD detectors, installed approx. one year ago, is now showing the same phenomena. With the experience of the first detector set in mind, a relative low beam current was selected (10-20nA resulting in approx 60.000 counts/sec). According to the supplier, X-ray photons can permanently damage the crystal structure resulting in the phenomena described. The guaranteed number of counts hitting the detector without causing damaging is 1.0E+12. This number seems to be enormous, but the reality is, using a detector at 60.000 counts/sec, 24 hrs/day, 7 days/wk, it can be used in an optimal condition for only half a year. Even just doing imaging on the microscope is contributing to the crystal damage.
We like to warn SDD users and ask if anyone else has experienced this phenomenon.
Several months back there was a request made on the Listserv for directions on how to maintain MT2 and MT2B ultramicrotomes. I offered to write out the directions and send them to anyone who wished a copy. It was not put on the Listserv. For there were only 4 or 5 who requested the information.
I have lost (probably deleted by mistake) my copy and would like to have it again. If any of you have that file please send me a copy. There has been another request for it already and do not have the time to recreate the file.
Thanks, Pat
Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
==============================Original Headers============================== 7, 27 -- From connellyps-at-nhlbi.nih.gov Tue Oct 25 09:57:22 2011 7, 27 -- Received: from nihxway4out.hub.nih.gov (nihxway4out.hub.nih.gov [128.231.90.112]) 7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9PEvMwY005624 7, 27 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 09:57:22 -0500 7, 27 -- X-IronPortListener: Outbound_SMTP 7, 27 -- X-IronPort-Anti-Spam-Filtered: true 7, 27 -- X-IronPort-Anti-Spam-Result: Au4HADTNpk6cKEcV/2dsb2JhbAA/A6kXfgeBdRJ1BAEVJwsiBBMUEgEEEyKcKoQtm2UChSyDFQSHVoRUh1+FS4wu 7, 27 -- Received: from unknown (HELO NIHHT02.nih.gov) ([156.40.71.21]) 7, 27 -- by nihxway4out.hub.nih.gov with ESMTP; 25 Oct 2011 10:57:22 -0400 7, 27 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by NIHHT02.nih.gov 7, 27 -- ([156.40.71.21]) with mapi; Tue, 25 Oct 2011 10:57:22 -0400 7, 27 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 7, 27 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 27 -- Date: Tue, 25 Oct 2011 10:57:21 -0400 7, 27 -- Subject: MT2 maintenance directions from Pat 7, 27 -- Thread-Topic: MT2 maintenance directions from Pat 7, 27 -- Thread-Index: AcyTJmWh7Cyr7zEaRve6s8Fw2/7Brw== 7, 27 -- Message-ID: {CACC4691.907E%connellyps-at-nhlbi.nih.gov} 7, 27 -- Accept-Language: en-US 7, 27 -- Content-Language: en 7, 27 -- X-MS-Has-Attach: 7, 27 -- X-MS-TNEF-Correlator: 7, 27 -- acceptlanguage: en-US 7, 27 -- Content-Type: text/plain; charset="iso-8859-1" 7, 27 -- MIME-Version: 1.0 7, 27 -- Content-Transfer-Encoding: 8bit 7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9PEvMwY005624 ==============================End of - Headers==============================
I can't say we've noticed this problem ourselves, but let me pose a tangentially related question to the listserver and the community.
We currently use a Philips CM200 (S)TEM equipped with a Si(Li) detector to support our irradiated materials programs. We are hoping to replace the CM in the indeterminate future with a new (S)TEM tool, and the new tool will certainly have one or more SDD chips.
Has anyone experimented with or thought about the effect hot betas or gammas would have on an SDD? We would probably not allow alpha-radioactive materials (i.e., fuels) into a new microscope, but we routinely see highly activated beta/gamma specimens that effect dead times, count rates, etc., on the Si(Li) detector. I would be interested in anyone's thoughts or experience of SDDs in a beta/gamma environment.
Does anyone know any papers that might discuss protocols or considerations for EDS analysis of toasty samples? I would appreciate citations if any pop to mind. Thanks!
Chad Parish ORNL
------- Email: corrie.van-hoek-at-tatasteel.com Name: Corrie van Heok
Organization: Tata Steel Europe
Title-Subject: [Filtered] Peak position, shape and resolution deterioration using Silicon Drift Detectors
Message: We use Thermo SDD detectors mounted on a JEOL-7001F microscope for rapid collection of X-rays for Spectral Imaging, Inclusion analysis and light element analysis. A first detector set was showing, within a year, a peak shift, a strong tailing on the left side of peak and a decrease in peak resolution in the low energy range (0-1keV) and has therefore replaced. The 2nd set of SDD detectors, installed approx. one year ago, is now showing the same phenomena. With the experience of the first detector set in mind, a relative low beam current was selected (10-20nA resulting in approx 60.000 counts/sec). According to the supplier, X-ray photons can permanently damage the crystal structure resulting in the phenomena described. The guaranteed number of counts hitting the detector without causing damaging is 1.0E+12. This number seems to be enormous, but the reality is, using a detector at 60.000 counts/sec, 24 hrs/day, 7 days/wk, it can be used in an optimal condition for only half a year. Even just doing imaging on the microscope is contributing to the crystal damage.
We like to warn SDD users and ask if anyone else has experienced this phenomenon.
==============================Original Headers============================== 15, 38 -- From parishcm-at-ornl.gov Tue Oct 25 10:30:22 2011 15, 38 -- Received: from emroute4.ornl.gov (emroute4.ornl.gov [160.91.86.27]) 15, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9PFUMFT022775 15, 38 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 10:30:22 -0500 15, 38 -- Received: from emroute4.ornl.gov ([127.0.0.1]) 15, 38 -- by emroute4.ornl.gov (PMDF V6.5-x6 #31890) 15, 38 -- with ESMTP id {0LTM00D92OELJV-at-emroute4.ornl.gov} for 15, 38 -- microscopy-at-microscopy.com; Tue, 25 Oct 2011 11:30:22 -0400 (EDT) 15, 38 -- Received: from CONVERSION-DAEMON.emroute4.ornl.gov by emroute4.ornl.gov 15, 38 -- (PMDF V6.5-x6 #31890) id {0LTM00E01OELC7-at-emroute4.ornl.gov} for 15, 38 -- microscopy-at-microscopy.com; Tue, 25 Oct 2011 11:30:21 -0400 (EDT) 15, 38 -- Received: from exchhub2.ornl.gov (exchhub2.ornl.gov [160.91.2.112]) 15, 38 -- by emroute4.ornl.gov (PMDF V6.5-x6 #31890) 15, 38 -- with ESMTPS id {0LTM00D8KOELK1-at-emroute4.ornl.gov} for 15, 38 -- microscopy-at-microscopy.com; Tue, 25 Oct 2011 11:30:21 -0400 (EDT) 15, 38 -- Received: from EXCHMBB.ornl.gov ([192.168.3.201]) 15, 38 -- by exchhub2.ornl.gov ([160.91.2.112]) with mapi; Tue, 15, 38 -- 25 Oct 2011 11:30:21 -0400 15, 38 -- Date: Tue, 25 Oct 2011 11:30:20 -0400 15, 38 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 15, 38 -- Subject: RE: [Microscopy] viaWWW:Peak position, 15, 38 -- shape and resolution deterioration using Silicon 15, 38 -- In-reply-to: {201110251218.p9PCIuIN032259-at-ns.microscopy.com} 15, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 15, 38 -- Message-id: {3C47B404D340EE48A469ADAE2ADBF96656B96BEDD9-at-EXCHMBB.ornl.gov} 15, 38 -- MIME-version: 1.0 15, 38 -- Content-type: text/plain; charset=us-ascii 15, 38 -- Content-language: en-US 15, 38 -- Thread-Topic: [Microscopy] viaWWW:Peak position, 15, 38 -- shape and resolution deterioration using Silicon 15, 38 -- Thread-Index: AcyTEEkF0HQmaNLWSl+Hh906qc/KvQAGMJwg 15, 38 -- Accept-Language: en-US 15, 38 -- acceptlanguage: en-US 15, 38 -- X-MS-Has-Attach: 15, 38 -- X-MS-TNEF-Correlator: 15, 38 -- References: {201110251218.p9PCIuIN032259-at-ns.microscopy.com} 15, 38 -- Content-Transfer-Encoding: 8bit 15, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9PFUMFT022775 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jmircheski-at-us.es as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Dear Listers, I hope you can help me yet again with a small doubt I have. I'm analyzing some neuro-muscular junctions, and I am quantifying the vesicles present in there. Apart from the synaptic vesicles, I'm interested in vesicles in general (lysosomes, endosomes, vacuoles etc. anything vesicular) and I'm measuring their size. So I get values ranging from some 20 or less nm up to hundreds of nms (radius).
The question is: if only looking at the values (area/diameter/radius), can one tell which vesicle is a synaptic vesicle and which one is something else (doesn't have to be strictly defined, just grouped in "other")? Is there any "limit" in a size parameter for a vesicle to be considered as a synaptic? In some literature: average diameter 40nm. I remember that there are some at 60nm, too, if I'm not wrong. What about the border values? What about 80, or 90 or 100 nm? I should stop writing now as more and more questions are coming out.... Waiting for your help. Regards, Josif P.S. I am sure there must be some old (or new) literature on this, just I can't find anything today.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman-at-pacific.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman-at-pacific.edu Name: Barbara Plowman
Organization: University Of the Pacific Title-Subject: [Filtered] "Scientific Terrorism"
Message: The waste media should be treated with 9% bleach which will kill the virus.
Only because I'm interested, is that a 9% solution of commercial bleach or a solution containing 9% sodium hypochlorite? As an side note, I once titrated a commercial solution of a national brand containing 6% sodium hypochlorite and found only 3%. I simply went out an bought a different case of the bleach from a store and it had 6%.
Frank
Email: bplowman-at-pacific.edu Name: Barbara Plowman
Organization: University Of the Pacific Title-Subject: [Filtered] "Scientific Terrorism"
Message: The waste media should be treated with 9% bleach which will kill the virus.
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 11, 26 -- From frank_karl-at-ardl.com Tue Oct 25 13:09:57 2011 11, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 11, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9PI9uFY010565 11, 26 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 13:09:56 -0500 11, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 11, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Tue, 25 11, 26 -- Oct 2011 14:09:55 -0400 11, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 11, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 11, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 11, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 11, 26 -- Date: Tue, 25 Oct 2011 14:09:55 -0400 11, 26 -- Subject: was Scientific Terrorism now bleach content 11, 26 -- Thread-Topic: was Scientific Terrorism now bleach content 11, 26 -- Thread-Index: AcyTPzJ+yPTpQTesSfGT2qBESGlD7gAAUDaA 11, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010B0D13B0-at-exchange2k7.ad.ardl.com} 11, 26 -- In-Reply-To: {201110251754.p9PHsd4F008002-at-ns.microscopy.com} 11, 26 -- Accept-Language: en-US 11, 26 -- Content-Language: en-US 11, 26 -- X-MS-Has-Attach: 11, 26 -- X-MS-TNEF-Correlator: 11, 26 -- acceptlanguage: en-US 11, 26 -- Content-Type: text/plain; charset="iso-8859-1" 11, 26 -- MIME-Version: 1.0 11, 26 -- Content-Transfer-Encoding: 8bit 11, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9PI9uFY010565 ==============================End of - Headers==============================
Back in the early 70's I was Freeze Etching and the procedure for cleaning the replicas called for 9% Sodium hypochlorite. Went crazy until I saw the label of my bottle of Chlorox bleach near my washing machine - 9%! It worked great for a while and then the digestion was not working so well. The grapevine told me that bleach looses its "umph" as it gets old so I bought a new bottle and all worked as well as I had hoped. Maybe the 3% Karl had was just an old bottle of 6%.
Pat Patricia Stranen Connelly Research Assistant NHLBI Electron Microscopy Core National Institutes of Health 14 Service Road West Bldg. 14E - Rm. 111B MSC 5570 Bethesda, MD 20892-5570 Phone 301-496-3491 FAX 301-402-0170 connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.
Around me everybody is working with mineralized tissue (School of Dentistry, you know...) "Etch and bleach" procedure is quite popular, and its purpose is to observe etched surface of mineral when it is free of collagen. From time to time I am getting from graduate students specimens, which were not bleached properly and some collagen still remained on surface. Every time it was caused by old bottle of sodium hypochlorite (reagent). I recommend (and use for my specimens) household bleach, and I mean household bleach: I bring it from my home in my special small "bleach bottle" when needed. Our washing machine works hard to keep it always fresh.
Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: connellyps-at-nhlbi.nih.gov [mailto:connellyps-at-nhlbi.nih.gov] } Sent: Tuesday, October 25, 2011 2:32 PM } To: Dusevich, Vladimir } Subject: [Microscopy] Re: was Scientific Terrorism now bleach content } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } Back in the early 70's I was Freeze Etching and the procedure for } cleaning the replicas called for 9% Sodium hypochlorite. Went crazy } until I saw the label of my bottle of Chlorox bleach near my washing } machine - 9%! It worked great for a while and then the digestion was } not working so well. The grapevine told me that bleach looses its } "umph" as it gets old so I bought a new bottle and all worked as well } as I had hoped. Maybe the 3% Karl had was just an old bottle of 6%. } } Pat } Patricia Stranen Connelly } Research Assistant } NHLBI Electron Microscopy Core } National Institutes of Health } 14 Service Road West } Bldg. 14E - Rm. 111B MSC 5570 } Bethesda, MD 20892-5570 } Phone 301-496-3491 } FAX 301-402-0170 } connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov} } } Opinions and experiences related are those of Pat Connelly and do not } represent the NIH. This message is not confidential and can be freely } shared and reproduced. } } } ________________________________ } X-from: {frank_karl-at-ardl.com} } Reply-To: {frank_karl-at-ardl.com} } Date: Tue, 25 Oct 2011 14:12:47 -0400 } To: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} } Subject: [Microscopy] was Scientific Terrorism now bleach content } } } Only because I'm interested, is that a 9% solution of commercial bleach } or a solution containing 9% sodium hypochlorite? As an side note, I } once titrated a commercial solution of a national brand containing 6% } sodium hypochlorite and found only 3%. I simply went out an bought a } different case of the bleach from a store and it had 6%. } } Frank } } } } Email: bplowman-at-pacific.edu Name: Barbara Plowman } } Organization: University Of the Pacific } Title-Subject: [Filtered] "Scientific Terrorism" } } Message: The waste media should be treated with 9% bleach which will } kill the virus. } } } } } ==============================Original } Headers============================== } 16, 28 -- From connellyps-at-nhlbi.nih.gov Tue Oct 25 14:31:25 2011 } 16, 28 -- Received: from nihxway4out.hub.nih.gov } (nihxway4out.hub.nih.gov [128.231.90.112]) } 16, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id p9PJVOQl028236 } 16, 28 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 14:31:25 } -0500 } 16, 28 -- X-IronPortListener: Outbound_SMTP } 16, 28 -- X-IronPort-Anti-Spam-Filtered: true } 16, 28 -- X-IronPort-Anti-Spam-Result: } AmoHAKkNp06cKEdK/2dsb2JhbABAA6kcfgeBbgECBBJ1BAEIDQQDAQIdCyIEExQJCAEBBBM } bB5w8hC2bbAKFP4MVBIdWhFSHYIVLjC8 } 16, 28 -- Received: from unknown (HELO NIHHTRC.nih.gov) } ([156.40.71.74]) } 16, 28 -- by nihxway4out.hub.nih.gov with ESMTP; 25 Oct 2011 15:31:24 } -0400 } 16, 28 -- Received: from NIHMLBX05.nih.gov ([156.40.71.35]) by } NIHHTRC.nih.gov } 16, 28 -- ([156.40.71.74]) with mapi; Tue, 25 Oct 2011 15:31:24 -0400 } 16, 28 -- From: "Connelly, Patricia (NIH/NHLBI) [E]" } {connellyps-at-nhlbi.nih.gov} } 16, 28 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 16, 28 -- Date: Tue, 25 Oct 2011 15:31:24 -0400 } 16, 28 -- Subject: Re: [Microscopy] was Scientific Terrorism now bleach } content } 16, 28 -- Thread-Topic: [Microscopy] was Scientific Terrorism now } bleach content } 16, 28 -- Thread-Index: AcyTQbTX+H5D4UkETuW/Gt6MfrBPHQACvmXT } 16, 28 -- Message-ID: {CACC86CC.90B5%connellyps-at-nhlbi.nih.gov} } 16, 28 -- In-Reply-To: {201110251812.p9PIClc5016868-at-ns.microscopy.com} } 16, 28 -- Accept-Language: en-US } 16, 28 -- Content-Language: en } 16, 28 -- X-MS-Has-Attach: } 16, 28 -- X-MS-TNEF-Correlator: } 16, 28 -- acceptlanguage: en-US } 16, 28 -- Content-Type: text/plain; charset="iso-8859-1" } 16, 28 -- MIME-Version: 1.0 } 16, 28 -- Content-Transfer-Encoding: 8bit } 16, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } ns.microscopy.com id p9PJVOQl028236 } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 29 -- From DusevichV-at-umkc.edu Tue Oct 25 15:40:19 2011 5, 29 -- Received: from mxtip01.um.umsystem.edu (mxtip01.um.umsystem.edu [209.106.229.20]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9PKeJdT013294 5, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 15:40:19 -0500 5, 29 -- X-IronPort-Anti-Spam-Filtered: true 5, 29 -- X-IronPort-Anti-Spam-Result: AiEBAG0dp07Raua1/2dsb2JhbAA/A5laj0N+B4FuAQEFOhQ3AgICAQgRAwEBAQ0CEAkHGxYBFAkIAQEECgkIEwIEh2erVokCAoU/gjRhBIgGngA 5, 29 -- Received: from um-thub01.um.umsystem.edu ([209.106.230.181]) 5, 29 -- by mxtip01-missouri-out.um.umsystem.edu with ESMTP; 25 Oct 2011 15:40:19 -0500 5, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.27]) by 5, 29 -- UM-THUB01.um.umsystem.edu ([209.106.230.181]) with mapi; Tue, 25 Oct 2011 5, 29 -- 15:40:19 -0500 5, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 29 -- Date: Tue, 25 Oct 2011 15:40:20 -0500 5, 29 -- Subject: RE: [Microscopy] Re: was Scientific Terrorism now bleach content 5, 29 -- Thread-Topic: [Microscopy] Re: was Scientific Terrorism now bleach content 5, 29 -- Thread-Index: AcyTTM0k59UbUPqTSOWkkcqtiaNpwQACUsxg 5, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3B7A7747E95-at-UM-EMAIL06.um.umsystem.edu} 5, 29 -- References: {201110251932.p9PJW9F1028968-at-ns.microscopy.com} 5, 29 -- In-Reply-To: {201110251932.p9PJW9F1028968-at-ns.microscopy.com} 5, 29 -- Accept-Language: en-US 5, 29 -- Content-Language: en-US 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- acceptlanguage: en-US 5, 29 -- Content-Type: text/plain; charset="us-ascii" 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9PKeJdT013294 ==============================End of - Headers==============================
Sodium hypochlorite degrades on storage. One statement on the net states: "The solution loses strength over time. The rate of degradation increases with heat and sunlight. Storage time is recommended to be less than 28 days. A max. storage life is about 60 to 90 days, depending on the solution storage temperature." Depending on how long the commercial bleach was sitting on shelf or in your lab, you would have observed the variation.
Sathya Srinivasan Research Associate Experimental Imaging Centre University of Calgary
==============================Original Headers============================== 6, 19 -- From sathya_sr70-at-hotmail.com Tue Oct 25 16:37:46 2011 6, 19 -- Received: from col0-omc3-s5.col0.hotmail.com (col0-omc3-s5.col0.hotmail.com [65.55.34.143]) 6, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9PLbjAG030528 6, 19 -- for {microscopy-at-microscopy.com} ; Tue, 25 Oct 2011 16:37:46 -0500 6, 19 -- Received: from COL118-W10 ([65.55.34.137]) by col0-omc3-s5.col0.hotmail.com with Microsoft SMTPSVC(6.0.3790.4675); 6, 19 -- Tue, 25 Oct 2011 14:37:45 -0700 6, 19 -- Message-ID: {COL118-W10CF92D7BA09C717D79C1580EC0-at-phx.gbl} 6, 19 -- X-Originating-IP: [136.159.165.234] 6, 19 -- From: Sathya Srinivasan {sathya_sr70-at-hotmail.com} 6, 19 -- To: {microscopy-at-microscopy.com} 6, 19 -- Subject: =?windows-1256?Q?RE:_was_Sc?= =?windows-1256?Q?ientific_T?= 6, 19 -- =?windows-1256?Q?errorism_n?= =?windows-1256?Q?ow_bleach_?= 6, 19 -- =?windows-1256?Q?content=FE=FE?= 6, 19 -- Date: Tue, 25 Oct 2011 21:37:44 +0000 6, 19 -- Importance: Normal 6, 19 -- Content-Type: text/plain; charset="windows-1256" 6, 19 -- Content-Transfer-Encoding: 8bit 6, 19 -- MIME-Version: 1.0 6, 19 -- X-OriginalArrivalTime: 25 Oct 2011 21:37:45.0602 (UTC) FILETIME=[55687620:01CC935E] ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman-at-pacific.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman-at-pacific.edu Name: Barbara Plowman
Organization: University of the Pacific, Arthur A. Dugoni School of Dentistry
Title-Subject: [Filtered] Bleach
Message: We use a Fisher brand of bleach. Could that have been a quality control problem?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both michael.cammer-at-med.nyu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
Organization: Skirball Institute of Biomolecular Medicine
Title-Subject: [Filtered] Amscope camera question
Message: If anyone out there has the Amscope mt-5000, would you please give us a review privately. We are thinking of purchasing one for our fluorescent stereoscope.
http://store.amscope.com/mt5000-ccd-ck.html Captures 5.0MP (2580X1944) high resolution fluorescence microscopy images • Image Sensor: 2/3" Sony Color CCD, Model ICX282AQ • CCD Scan Mode: Interline • Pixel Size: 3.4micron x 3.4micron • Resolution: 2580(H) x 1944(V) Pixels • G Sensitivity: 280mV • Filter: RGB Bayer Pattern • Port: Standard C-Mount • Frame Rate: 3 fps -at- 2580x1944 pixels; 10 fps -at- 1280x932 pixels • Low-speed Readout: Yes • A/D conversion: 12 bit • Peltier Cooled: -30°C below ambient connects via USB
Thanks!!
________________________________________________________ Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine Lab: (212) 263-3208 Cell: (914) 309-3270
Hello Everyone, I have discovered our LKB 2178 knife maker II doesn't work. I know Ted Pella repairs them, but I was hoping to find someone east of the Mississippi River. Failing that, does anyone know were I can buy the cutting wheel and springs. I would be willing to give DIY a try, but I really need the replacement parts.
Thanks!!!!!!
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 7, 25 -- From frank_karl-at-ardl.com Wed Oct 26 13:16:34 2011 7, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9QIGXRx014483 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2011 13:16:33 -0500 7, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 7, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Wed, 26 7, 25 -- Oct 2011 14:16:32 -0400 7, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 7, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 7, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 7, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 7, 25 -- Date: Wed, 26 Oct 2011 14:16:32 -0400 7, 25 -- Subject: LKB glass knife maker 7, 25 -- Thread-Topic: LKB glass knife maker 7, 25 -- Thread-Index: AcyUC2O1hGYV7imvRLeWb2Cg/pazcA== 7, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010B0D13B1-at-exchange2k7.ad.ardl.com} 7, 25 -- Accept-Language: en-US 7, 25 -- Content-Language: en-US 7, 25 -- X-MS-Has-Attach: 7, 25 -- X-MS-TNEF-Correlator: 7, 25 -- acceptlanguage: en-US 7, 25 -- Content-Type: text/plain; charset="iso-8859-1" 7, 25 -- MIME-Version: 1.0 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9QIGXRx014483 ==============================End of - Headers==============================
Hello Frank, I have the manuals of LKB how to set things right with the knifemaker. It`s "easy"; mostly cleaning parts from glass particles, greasing new, doing some minor adjustments. Email me for the link.
Best regards, Stefan
----------------------------------------------------- Stefan Diller - Wissenschaftliche Photographie Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 26.10.11 20:22, schrieb frank_karl-at-ardl.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello Everyone, } I have discovered our LKB 2178 knife maker II doesn't work. I know Ted Pella repairs them, but I was hoping to find someone east of the Mississippi River. Failing that, does anyone know were I can buy the cutting wheel and springs. I would be willing to give DIY a try, but I really need the replacement parts. } } Thanks!!!!!! } } Frank Karl } Microscopist } ARDL } 2887 Gilchrist Road } Akron, Ohio 44305 } 330-794-6600 } } } } ________________________________ } This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc. } } } ==============================Original Headers============================== } 7, 25 -- From frank_karl-at-ardl.com Wed Oct 26 13:16:34 2011 } 7, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) } 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9QIGXRx014483 } 7, 25 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2011 13:16:33 -0500 } 7, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by } 7, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Wed, 26 } 7, 25 -- Oct 2011 14:16:32 -0400 } 7, 25 -- From: Frank Karl {frank_karl-at-ardl.com} } 7, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} } 7, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} } 7, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} } 7, 25 -- Date: Wed, 26 Oct 2011 14:16:32 -0400 } 7, 25 -- Subject: LKB glass knife maker } 7, 25 -- Thread-Topic: LKB glass knife maker } 7, 25 -- Thread-Index: AcyUC2O1hGYV7imvRLeWb2Cg/pazcA== } 7, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010B0D13B1-at-exchange2k7.ad.ardl.com} } 7, 25 -- Accept-Language: en-US } 7, 25 -- Content-Language: en-US } 7, 25 -- X-MS-Has-Attach: } 7, 25 -- X-MS-TNEF-Correlator: } 7, 25 -- acceptlanguage: en-US } 7, 25 -- Content-Type: text/plain; charset="iso-8859-1" } 7, 25 -- MIME-Version: 1.0 } 7, 25 -- Content-Transfer-Encoding: 8bit } 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9QIGXRx014483 } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 22 -- From stefan.diller-at-t-online.de Wed Oct 26 13:51:24 2011 6, 22 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9QIpOgI031540 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 26 Oct 2011 13:51:24 -0500 6, 22 -- Received: from fwd02.aul.t-online.de (fwd02.aul.t-online.de ) 6, 22 -- by mailout08.t-online.de with smtp 6, 22 -- id 1RJ8ZP-0003MK-71; Wed, 26 Oct 2011 20:51:23 +0200 6, 22 -- Received: from mac-pro.speedport.ip (bd18DrZX8hlL0tsAqDf5o7Fo4unTcZK3D5inVnSlc7sSM601vIdaUx2zmWtINAxglk-at-[93.222.106.110]) by fwd02.t-online.de 6, 22 -- with esmtp id 1RJ8ZI-02XHyS0; Wed, 26 Oct 2011 20:51:16 +0200 6, 22 -- Message-ID: {4EA856A3.3060602-at-t-online.de} 6, 22 -- Date: Wed, 26 Oct 2011 20:51:15 +0200 6, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 6, 22 -- MIME-Version: 1.0 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] LKB glass knife maker 6, 22 -- References: {201110261822.p9QIMOIM020440-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {201110261822.p9QIMOIM020440-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-ID: bd18DrZX8hlL0tsAqDf5o7Fo4unTcZK3D5inVnSlc7sSM601vIdaUx2zmWtINAxglk 6, 22 -- X-TOI-MSGID: fb634f98-50dc-4adf-a93c-74e98f792d59 ==============================End of - Headers==============================
I have a couple of cutting wheels and axles, and damping pads, for a LKB 7801 B. If they'll fit, you can have them. No springs, though.
Phil
} Hello Everyone, } I have discovered our LKB 2178 knife maker II doesn't work. I know } Ted Pella repairs them, but I was hoping to find someone east of the } Mississippi River. Failing that, does anyone know were I can buy } the cutting wheel and springs. I would be willing to give DIY a } try, but I really need the replacement parts. } } Thanks!!!!!! } } Frank Karl } Microscopist } ARDL } 2887 Gilchrist Road } Akron, Ohio 44305 } 330-794-6600
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 26 -- From oshel1pe-at-cmich.edu Wed Oct 26 14:59:41 2011 5, 26 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9QJxfkY016533 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Oct 2011 14:59:41 -0500 5, 26 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 5, 26 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id p9QJfUN5003095 5, 26 -- for {Microscopy-at-microscopy.com} ; Wed, 26 Oct 2011 15:41:30 -0400 5, 26 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 26 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Wed, 26 Oct 5, 26 -- 2011 15:59:40 -0400 5, 26 -- MIME-Version: 1.0 5, 26 -- Message-ID: {a06240800cace1558df47-at-[141.209.160.249]} 5, 26 -- In-Reply-To: {201110261821.p9QILrH7019495-at-ns.microscopy.com} 5, 26 -- References: {201110261821.p9QILrH7019495-at-ns.microscopy.com} 5, 26 -- Date: Wed, 26 Oct 2011 15:59:38 -0400 5, 26 -- To: {Microscopy-at-microscopy.com} 5, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 26 -- Subject: Re: [Microscopy] LKB glass knife maker 5, 26 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 26 -- X-Originating-IP: [141.209.160.249] 5, 26 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 26 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 5, 26 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 26 -- X-CanItPRO-Stream: default 5, 26 -- X-Canit-Stats-ID: 03FNvFu0j - 2d2b0323a0e9 - 20111026 5, 26 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: mlibbee-at-gmail.com Name: Marissa Mancuso
Organization: NCEM at LBL
Title-Subject: [Filtered] proper care of micropipette
Message: Listers-
Our prep labs were in need of a micropipette for drop casting nanoparticles and it has arrived. Before I make it available, I would like to get some suggestions on how to properly maintain it (e.g. how to store it btwn use) within a user facility to avoid issues of cross-contamination, etc. Thanks, Marissa
Dear Listers: We need the technical or repair manual (not operation manual)for our Olympus DP 12 -2 digital camera for optical microscope. It has an intermitent display on the control box and we suspect that it is an contact problem at the hinge. Either soft or hard copy will do. Thanks in advance.... Regards kiss
Dr Francisco José Kiss Universidade Federal do Rio Grande do Sul Escola de Engenharia - Dpto. de Metalurgia Av Osvaldo Aranha 99 S610 90046-900 Porto Alegre - RS e-mail: kiss-at-demet.ufrgs.br fone +55 51 3308 3414 {
==============================Original Headers============================== 5, 18 -- From kiss-at-demet.ufrgs.br Thu Oct 27 11:21:47 2011 5, 18 -- Received: from servidor.demet.ufrgs.br (servidor.demet.ufrgs.br [143.54.39.253]) 5, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9RGLk8m004776 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 11:21:47 -0500 5, 18 -- Received: from webmail.demet.ufrgs.br (localhost.localdomain [127.0.0.1]) 5, 18 -- by servidor.demet.ufrgs.br (8.13.8/8.12.10) with ESMTP id p9RH9gqR008905 5, 18 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 14:09:42 -0300 5, 18 -- From: "Francisco Jose Kiss" {kiss-at-demet.ufrgs.br} 5, 18 -- To: microscopy-at-microscopy.com 5, 18 -- Subject: Olympus DP12-2 Technical manual 5, 18 -- Date: Thu, 27 Oct 2011 14:09:42 -0300 5, 18 -- Message-Id: {20111027170135.M56461-at-demet.ufrgs.br} 5, 18 -- X-Mailer: OpenWebMail 2.52 20060502 5, 18 -- X-OriginatingIP: 143.54.39.2 (kiss) 5, 18 -- MIME-Version: 1.0 5, 18 -- Content-Type: text/plain; 5, 18 -- charset=iso-8859-1 5, 18 -- Received-SPF: pass (servidor.demet.ufrgs.br: domain of kiss-at-demet.ufrgs.br designates 127.0.0.1 as permitted sender) receiver=servidor.demet.ufrgs.br; client-ip=127.0.0.1; helo=webmail.demet.ufrgs.br; envelope-from=kiss-at-demet.ufrgs.br; x-software=spfmilter 0.97 http://www.acme.com/software/spfmilter/ with libspf2-1.0.0; ==============================End of - Headers==============================
I will have an experiment that will require relatively low carbon deposition rates, and I am expecting I'll need to impose "draconian" limitations on the microscope for a few days before doing this experiment. This is in a tungsten LV-SEM with a fairly large sample chamber. It's pumped by a scroll pump in LVac, and a scroll behind a turbo in HVac. We are already using gloves and leaving it in low-vac N2 overnight, etc., but I was wondering if you folks had any simple actions you've found successful in reducing the C contamination within your chambers over and above the usual vacuum maintenance activities, for example, not using carbon paint to mounts your samples, etc.
Thank you in advance!
Zack Gainsforth Space Sciences Laboratory, UC Berkeley 7 Gauss Way Berkeley, CA 94720 510-642-9733 zackg-at-ssl.berkeley.edu
==============================Original Headers============================== 9, 22 -- From zackg-at-berkeley.edu Thu Oct 27 16:22:52 2011 9, 22 -- Received: from cm06fe.IST.Berkeley.EDU (cm06fe.IST.Berkeley.EDU [169.229.218.147]) 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9RLMq9R025655 9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 16:22:52 -0500 9, 22 -- Received: from zackg-lap.ssl.berkeley.edu ([128.32.13.191]) 9, 22 -- by cm06fe.ist.berkeley.edu with esmtpsa (TLSv1:AES128-SHA:128) 9, 22 -- (Exim 4.76) 9, 22 -- (auth plain:zackg-at-berkeley.edu) 9, 22 -- (envelope-from {zackg-at-berkeley.edu} ) 9, 22 -- id 1RJXPX-0002TM-Lj 9, 22 -- for Microscopy-at-microscopy.com; Thu, 27 Oct 2011 14:22:52 -0700 9, 22 -- From: Zack Gainsforth {zackg-at-berkeley.edu} 9, 22 -- Content-Type: text/plain; charset=us-ascii 9, 22 -- Subject: SEM - Reducing carbon deposition 9, 22 -- Date: Thu, 27 Oct 2011 14:22:51 -0700 9, 22 -- References: {BF5A6C75-1DAF-4D85-9B60-FF60C3674870-at-ssl.berkeley.edu} 9, 22 -- To: Microscopy-at-microscopy.com 9, 22 -- Message-Id: {9F0F9A80-7BFA-4FB2-BF4F-0AF025D2821F-at-berkeley.edu} 9, 22 -- Mime-Version: 1.0 (Apple Message framework v1251.1) 9, 22 -- X-Mailer: Apple Mail (2.1251.1) 9, 22 -- Content-Transfer-Encoding: 8bit 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9RLMq9R025655 ==============================End of - Headers==============================
Hi Zack, When I bought my Cameca Sx100 about 6 years ago, the dollar had fallen against the Euro and I could no longer afford a turbo pumped system. But Cameca and I discussed a cryo baffle trap for the diffusion pump and they eventually supplied a "Cryo-Tiger" or "Aqua-Trap" system that runs at 100K degrees and was designed to pump water in a vacuum. Of course at that temperature it pumps hydrocarbons even better.
The compressor is air cooled and I just leave it on all the time, and although I was just expecting it to help with backstreaming from the diffusion pump, I am totally impressed with the degree that this cryo system not only eliminates backstreaming from the diffusion pump, but even stabilizes the carbon contamination signal on my instrument. Under normal conditions, I cannot detect an increase in carbon ka for many minutes and even then it's around 1 or 2 sigma.
I really like it and highly recommend it for others that don't have an ultra-high vacuum or "dry" pump system. john
At 02:28 PM 10/27/2011, zackg-at-berkeley.edu wrote:
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 25 -- From donovan-at-uoregon.edu Thu Oct 27 17:45:27 2011 10, 25 -- Received: from smtp.uoregon.edu (cc-mserv4.uoregon.edu [128.223.142.129]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9RMjQfk011889 10, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 17:45:27 -0500 10, 25 -- Received: from Probev.uoregon.edu (probev.uoregon.edu [128.223.10.46]) 10, 25 -- (authenticated bits=0) 10, 25 -- by smtp.uoregon.edu (8.14.5/8.14.5) with ESMTP id p9RMjIFK025595 10, 25 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NOT); 10, 25 -- Thu, 27 Oct 2011 15:45:19 -0700 10, 25 -- Message-Id: {201110272245.p9RMjIFK025595-at-smtp.uoregon.edu} 10, 25 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 10, 25 -- Date: Thu, 27 Oct 2011 15:45:18 -0700 10, 25 -- To: Microscopy-at-microscopy.com 10, 25 -- From: John Donovan {donovan-at-uoregon.edu} 10, 25 -- Subject: Re: [Microscopy] SEM - Reducing carbon deposition 10, 25 -- Cc: zackg-at-berkeley.edu 10, 25 -- In-Reply-To: {201110272128.p9RLSc8a032104-at-ns.microscopy.com} 10, 25 -- References: {201110272128.p9RLSc8a032104-at-ns.microscopy.com} 10, 25 -- Mime-Version: 1.0 10, 25 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 10, 25 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.4.6813,1.0.211,0.0.0000 10, 25 -- definitions=2011-10-27_06:2011-10-27,2011-10-27,1970-01-01 signatures=0 10, 25 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=48 10, 25 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=-45 10, 25 -- reason=mlx scancount=1 engine=6.0.2-1012030000 definitions=main-1110270280 ==============================End of - Headers==============================
Carbon itself does not evaporate or outgass in vacuum at room temperature, so it would be safe to use water-based Aquadag type of carbon paint for sample mounting.
What you want to avoid is anything and everything of organic or polymer nature that still can fly, float, or even slowly creep over the surface. Meaning - nothing sticky (no carbon pads, double-sided or any other sticky tapes, etc...), no glues (other then water-based Aquadag, TorrSeal, Hyson-1C, and Ted Pellas high-conductivity colloidal silver) no plastics (other then PEEK, Teflon, or kapton/polyimide), and the last but probably the most important - get the Evactron and run it for couple of hours before loading your sample and (if sample allows it) then for 15 minutes with the sample already in chamber...
If your sample can't stand oxygen plasma at all and therefore is not compatible with Evactron cleaning, then immediatelly before loading it to SEM you can treat the sample in UV/Ozone cleaner for couple of hours.
Ah, yes, make sure that SEM stage is/was lubricated only by something with critical vapor pressure in E-9 range (TorrLube, Y-25, or similar), otherwise outgassing of carbon-containing molecules from the stage lubrication will make all the precautions fairly irrelevant...
Good luck :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/27/2011 5:24 PM, zackg-at-berkeley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } I will have an experiment that will require relatively low carbon deposition rates, and I am expecting I'll need to impose "draconian" limitations on the microscope for a few days before doing this experiment. This is in a tungsten LV-SEM with a fairly large sample chamber. It's pumped by a scroll pump in LVac, and a scroll behind a turbo in HVac. We are already using gloves and leaving it in low-vac N2 overnight, etc., but I was wondering if you folks had any simple actions you've found successful in reducing the C contamination within your chambers over and above the usual vacuum maintenance activities, for example, not using carbon paint to mounts your samples, etc. } } Thank you in advance! } } Zack Gainsforth } Space Sciences Laboratory, UC Berkeley } 7 Gauss Way } Berkeley, CA 94720 } 510-642-9733 } zackg-at-ssl.berkeley.edu } } } } } } } ==============================Original Headers============================== } 9, 22 -- From zackg-at-berkeley.edu Thu Oct 27 16:22:52 2011 } 9, 22 -- Received: from cm06fe.IST.Berkeley.EDU (cm06fe.IST.Berkeley.EDU [169.229.218.147]) } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9RLMq9R025655 } 9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 16:22:52 -0500 } 9, 22 -- Received: from zackg-lap.ssl.berkeley.edu ([128.32.13.191]) } 9, 22 -- by cm06fe.ist.berkeley.edu with esmtpsa (TLSv1:AES128-SHA:128) } 9, 22 -- (Exim 4.76) } 9, 22 -- (auth plain:zackg-at-berkeley.edu) } 9, 22 -- (envelope-from {zackg-at-berkeley.edu} ) } 9, 22 -- id 1RJXPX-0002TM-Lj } 9, 22 -- for Microscopy-at-microscopy.com; Thu, 27 Oct 2011 14:22:52 -0700 } 9, 22 -- From: Zack Gainsforth {zackg-at-berkeley.edu} } 9, 22 -- Content-Type: text/plain; charset=us-ascii } 9, 22 -- Subject: SEM - Reducing carbon deposition } 9, 22 -- Date: Thu, 27 Oct 2011 14:22:51 -0700 } 9, 22 -- References: {BF5A6C75-1DAF-4D85-9B60-FF60C3674870-at-ssl.berkeley.edu} } 9, 22 -- To: Microscopy-at-microscopy.com } 9, 22 -- Message-Id: {9F0F9A80-7BFA-4FB2-BF4F-0AF025D2821F-at-berkeley.edu} } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v1251.1) } 9, 22 -- X-Mailer: Apple Mail (2.1251.1) } 9, 22 -- Content-Transfer-Encoding: 8bit } 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9RLMq9R025655 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 38 -- From vray-at-partbeamsystech.com Thu Oct 27 17:59:36 2011 8, 38 -- Received: from nm3.access.bullet.mail.mud.yahoo.com (nm3.access.bullet.mail.mud.yahoo.com [66.94.237.204]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9RMxap5030047 8, 38 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 17:59:36 -0500 8, 38 -- Received: from [66.94.237.199] by nm3.access.bullet.mail.mud.yahoo.com with NNFMP; 27 Oct 2011 22:59:36 -0000 8, 38 -- Received: from [98.139.221.62] by tm10.access.bullet.mail.mud.yahoo.com with NNFMP; 27 Oct 2011 22:59:36 -0000 8, 38 -- Received: from [127.0.0.1] by smtp103.biz.mail.bf1.yahoo.com with NNFMP; 27 Oct 2011 22:59:36 -0000 8, 38 -- X-Yahoo-Newman-Id: 147102.16439.bm-at-smtp103.biz.mail.bf1.yahoo.com 8, 38 -- X-Yahoo-Newman-Property: ymail-3 8, 38 -- X-YMail-OSG: R0S5u5MVM1mWu0hhWT56DJp6b11NTbyqGaba0nkM82F3CI6 8, 38 -- tqFDBCYuUWT6QFoDxMiSe3LTLxULpFWhCeIGpOJq1YeBFxEO4aLqbnEKSRCf 8, 38 -- 8yhMdazTtmnsd9sA8jDTH5QHugI6pBgyofzW2VWvooOJ6IgnC.9mWn9x9mW6 8, 38 -- 6YGRjzHOE3Em7Wjvj2HurWDCtYhcMc72obfQJuR_PwbGmcgepWDe293eR0BJ 8, 38 -- re7Ov2UCUtXVaNJlWJscbmHihcE4ygEcG9nla.yLZx0Hr8fwkUBu2g9z6Hdw 8, 38 -- 3TcAVpTjyyHqJ6ehhfXaHsFAOei8UAw0h6nhqEunUZPZpZ3mjcntJafwKRqg 8, 38 -- TdquLRvAZDkwpStI6l.iiKmIaDEAiMwOnbpZgXFz_PXeLI6UvVvrAGAIMOZd 8, 38 -- ICZ7AUSBNcABzE5gkA24WFBdbIjTZk9REEbcFx5sauAvybGMMMl4AxE745UQ 8, 38 -- 5jrhE5kiuaHNX0G23vMSAf6keef.QV.Y2v5kri1sMhD0h2gp6h.dIgUWuOXd 8, 38 -- F5.I3Ncin0RXJbch9DRuwLFPHcwsHBECNncdQDBWetO5yYv_g_4Lzoi_BbA0 8, 38 -- Oq.3HG6sAVpKxtdw1vg17pETSL0PK5toJ8xJRmORcRFPHlmaYVbwNbVgtSiy 8, 38 -- wLyXpqhcMYkYTZy5Bom70T_4y5Io- 8, 38 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 8, 38 -- Received: from [192.168.5.252] (vray-at-64.134.30.249 with plain) 8, 38 -- by smtp103.biz.mail.bf1.yahoo.com with SMTP; 27 Oct 2011 15:59:35 -0700 PDT 8, 38 -- Message-ID: {4EA9E257.9080307-at-partbeamsystech.com} 8, 38 -- Date: Thu, 27 Oct 2011 18:59:35 -0400 8, 38 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 8, 38 -- Reply-To: vray-at-partbeamsystech.com 8, 38 -- Organization: PBS&T 8, 38 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 8, 38 -- MIME-Version: 1.0 8, 38 -- To: zackg-at-berkeley.edu 8, 38 -- CC: microscopy-at-microscopy.com 8, 38 -- Subject: Re: [Microscopy] SEM - Reducing carbon deposition 8, 38 -- References: {201110272124.p9RLOMdd026953-at-ns.microscopy.com} 8, 38 -- In-Reply-To: {201110272124.p9RLOMdd026953-at-ns.microscopy.com} 8, 38 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Carbon itself does not evaporate or outgass in vacuum at room temperature, so it would be safe to use water-based Aquadag type of carbon paint for sample mounting.
What you want to avoid is anything and everything of organic or polymer nature that still can fly, float, or even slowly creep over the surface. Meaning - nothing sticky (no carbon pads, double-sided or any other sticky tapes, etc...), no glues (other then water-based Aquadag, TorrSeal, Hyson-1C, and Ted Pellas high-conductivity colloidal silver) no plastics (other then PEEK, Teflon, or kapton/polyimide), and the last but probably the most important - get the Evactron and run it for couple of hours before loading your sample and (if sample allows it) then for 15 minutes with the sample already in chamber...
If your sample can't stand oxygen plasma at all and therefore is not compatible with Evactron cleaning, then immediatelly before loading it to SEM you can treat the sample in UV/Ozone cleaner for couple of hours.
Ah, yes, make sure that SEM stage is/was lubricated only by something with critical vapor pressure in E-9 range (TorrLube, Y-25, or similar), otherwise outgassing of carbon-containing molecules from the stage lubrication will make all the precautions fairly irrelevant...
Good luck :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/27/2011 5:24 PM, zackg-at-berkeley.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi All, } } I will have an experiment that will require relatively low carbon deposition rates, and I am expecting I'll need to impose "draconian" limitations on the microscope for a few days before doing this experiment. This is in a tungsten LV-SEM with a fairly large sample chamber. It's pumped by a scroll pump in LVac, and a scroll behind a turbo in HVac. We are already using gloves and leaving it in low-vac N2 overnight, etc., but I was wondering if you folks had any simple actions you've found successful in reducing the C contamination within your chambers over and above the usual vacuum maintenance activities, for example, not using carbon paint to mounts your samples, etc. } } Thank you in advance! } } Zack Gainsforth } Space Sciences Laboratory, UC Berkeley } 7 Gauss Way } Berkeley, CA 94720 } 510-642-9733 } zackg-at-ssl.berkeley.edu } } } } } } } ==============================Original Headers============================== } 9, 22 -- From zackg-at-berkeley.edu Thu Oct 27 16:22:52 2011 } 9, 22 -- Received: from cm06fe.IST.Berkeley.EDU (cm06fe.IST.Berkeley.EDU [169.229.218.147]) } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9RLMq9R025655 } 9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 16:22:52 -0500 } 9, 22 -- Received: from zackg-lap.ssl.berkeley.edu ([128.32.13.191]) } 9, 22 -- by cm06fe.ist.berkeley.edu with esmtpsa (TLSv1:AES128-SHA:128) } 9, 22 -- (Exim 4.76) } 9, 22 -- (auth plain:zackg-at-berkeley.edu) } 9, 22 -- (envelope-from {zackg-at-berkeley.edu} ) } 9, 22 -- id 1RJXPX-0002TM-Lj } 9, 22 -- for Microscopy-at-microscopy.com; Thu, 27 Oct 2011 14:22:52 -0700 } 9, 22 -- From: Zack Gainsforth {zackg-at-berkeley.edu} } 9, 22 -- Content-Type: text/plain; charset=us-ascii } 9, 22 -- Subject: SEM - Reducing carbon deposition } 9, 22 -- Date: Thu, 27 Oct 2011 14:22:51 -0700 } 9, 22 -- References: {BF5A6C75-1DAF-4D85-9B60-FF60C3674870-at-ssl.berkeley.edu} } 9, 22 -- To: Microscopy-at-microscopy.com } 9, 22 -- Message-Id: {9F0F9A80-7BFA-4FB2-BF4F-0AF025D2821F-at-berkeley.edu} } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v1251.1) } 9, 22 -- X-Mailer: Apple Mail (2.1251.1) } 9, 22 -- Content-Transfer-Encoding: 8bit } 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9RLMq9R025655 } ==============================End of - Headers============================== }
==============================Original Headers============================== 8, 38 -- From vray-at-partbeamsystech.com Thu Oct 27 18:00:28 2011 8, 38 -- Received: from nm23-vm0.access.bullet.mail.mud.yahoo.com (nm23-vm0.access.bullet.mail.mud.yahoo.com [66.94.236.141]) 8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9RN0Rox032136 8, 38 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 18:00:27 -0500 8, 38 -- Received: from [66.94.237.127] by nm23.access.bullet.mail.mud.yahoo.com with NNFMP; 27 Oct 2011 23:00:27 -0000 8, 38 -- Received: from [98.139.221.63] by tm2.access.bullet.mail.mud.yahoo.com with NNFMP; 27 Oct 2011 23:00:27 -0000 8, 38 -- Received: from [127.0.0.1] by smtp104.biz.mail.bf1.yahoo.com with NNFMP; 27 Oct 2011 23:00:27 -0000 8, 38 -- X-Yahoo-Newman-Id: 378878.66927.bm-at-smtp104.biz.mail.bf1.yahoo.com 8, 38 -- X-Yahoo-Newman-Property: ymail-3 8, 38 -- X-YMail-OSG: HWCvGG4VM1mg3LXnKrswt2AdAdD9BsVHBExO36U36JHWpTY 8, 38 -- 6w4VaMPX2_UdcPlJ.D2uJHn2QYvRxCEQ0CU1e3c_N0Toeqk4_zgra9MPatyU 8, 38 -- lz.BdC.M4GKA5W0Jn70XSU_WlSPXXl1I.tz4rqC82LC0W5bYocl8CN9CcG9Y 8, 38 -- ZKjvPZFJZfiIki2p2JuNTUvb65775xyOiBH2EzKTLBcCdZonjjK0Dt9FTNOf 8, 38 -- rnlIyJXXhDMlHTXYpU_OF67P6WNEm.kUJZstYx8LmxN7ioPtFPRv3M.NtQh8 8, 38 -- dmcU__8hDuxt7pkAJjy.VbgOfXDrhLH.2k5QrPKZY2jzL2AaD5kfAIRMJEPw 8, 38 -- MZPVc7B5zrStebScBY8BRAwA7ce_RVdqE2jT7iKSOWs2xeVe5nG.aTRBunBq 8, 38 -- Xdkv.s9paC6J5HdX1jQttSdFiXYQOH6q_Jls.ZN27.dYdRKQsXnWIEI7lG3X 8, 38 -- HuwLljoDfz_AnLX1LQ3boLr3TuaHb.2wAQyJZLEQfQkpG1.PPaX9tswY8293 8, 38 -- j1dGMi9TVWSFO.FKWe5wFpZMVo0i5EALI9hF8.wRgqo7mFFoOwCPlPh8Ut2M 8, 38 -- gl3l8f8IBAXUSEubzW8.9D_Qk2olWb2TvJTWhRCXHIyTV0CCxt59nlz.v96Y 8, 38 -- kYTjzoPQFmnFLh.pli5s1H6HoSgJs 8, 38 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 8, 38 -- Received: from [192.168.5.252] (vray-at-64.134.30.249 with plain) 8, 38 -- by smtp104.biz.mail.bf1.yahoo.com with SMTP; 27 Oct 2011 16:00:26 -0700 PDT 8, 38 -- Message-ID: {4EA9E28A.6050807-at-partbeamsystech.com} 8, 38 -- Date: Thu, 27 Oct 2011 19:00:26 -0400 8, 38 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 8, 38 -- Reply-To: vray-at-partbeamsystech.com 8, 38 -- Organization: PBS&T 8, 38 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 8, 38 -- MIME-Version: 1.0 8, 38 -- To: zackg-at-berkeley.edu 8, 38 -- CC: microscopy-at-microscopy.com 8, 38 -- Subject: Re: [Microscopy] SEM - Reducing carbon deposition 8, 38 -- References: {201110272124.p9RLOMdd026953-at-ns.microscopy.com} 8, 38 -- In-Reply-To: {201110272124.p9RLOMdd026953-at-ns.microscopy.com} 8, 38 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 8, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
You are welcome. The difference between water-based aquadag and carbon tape comes from the fact that tape contains carbon and the glue.
Carbon itself is a solid and its critical vapor pressure at room temperature should be quite low. I do not remember exact value of CVP of C, and am out of my office to look it up at this moment, but I do know C must be heated to 2500K to get it sublimate in vacuum. This tells me that C should not be volatile at 273K. Solid carbon therefore has no way to migrate onto your sample and show up in the experiment, even if it is present in the chamber. That is why water-based Aquagad paint should be safe - it contains only carbon powder and DI water.
Glue however is a very different story. Almost any glue will contain some un-polymerized organic molecules, which will outgass (even if that is with extremely low partial pressures) and make a way to the area where electron beam hits the sample. Most organic molecules would be broken by electron beam radiation and will deposit carbon in some quantities...
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/27/2011 7:03 PM, Zack Gainsforth wrote: } Hi Valery, } } Just what I was looking for! I will check into these points and let you know what I find. I find it interesting you think carbon paint is OK, but tape isn't. I'll do an experiment and see if it is in fact cleaner in my system. } } Thank you very much! } } Zack } } } } } On Oct 27, 2011, at 3:59 PM, vray-at-partbeamsystech.com wrote: } } Hi Zak, } } Carbon itself does not evaporate or outgass in vacuum at room temperature, so it would be safe to use water-based Aquadag type of carbon paint for sample mounting. } } What you want to avoid is anything and everything of organic or polymer nature that still can fly, float, or even slowly creep over the surface. Meaning - nothing sticky (no carbon pads, double-sided or any other sticky tapes, etc...), no glues (other then water-based Aquadag, TorrSeal, Hyson-1C, and Ted Pellas high-conductivity colloidal silver) no plastics (other then PEEK, Teflon, or kapton/polyimide), and the last but probably the most important - get the Evactron and run it for couple of hours before loading your sample and (if sample allows it) then for 15 minutes with the sample already in chamber... } } If your sample can't stand oxygen plasma at all and therefore is not compatible with Evactron cleaning, then immediatelly before loading it to SEM you can treat the sample in UV/Ozone cleaner for couple of hours. } } Ah, yes, make sure that SEM stage is/was lubricated only by something with critical vapor pressure in E-9 range (TorrLube, Y-25, or similar), otherwise outgassing of carbon-containing molecules from the stage lubrication will make all the precautions fairly irrelevant... } } Good luck :) } } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 10/27/2011 5:24 PM, zackg-at-berkeley.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi All, } } } } I will have an experiment that will require relatively low carbon deposition rates, and I am expecting I'll need to impose "draconian" limitations on the microscope for a few days before doing this experiment. This is in a tungsten LV-SEM with a fairly large sample chamber. It's pumped by a scroll pump in LVac, and a scroll behind a turbo in HVac. We are already using gloves and leaving it in low-vac N2 overnight, etc., but I was wondering if you folks had any simple actions you've found successful in reducing the C contamination within your chambers over and above the usual vacuum maintenance activities, for example, not using carbon paint to mounts your samples, etc. } } } } Thank you in advance! } } } } Zack Gainsforth } } Space Sciences Laboratory, UC Berkeley } } 7 Gauss Way } } Berkeley, CA 94720 } } 510-642-9733 } } zackg-at-ssl.berkeley.edu } } } } } } } } } } } } } } ==============================Original Headers============================== } } 9, 22 -- From zackg-at-berkeley.edu Thu Oct 27 16:22:52 2011 } } 9, 22 -- Received: from cm06fe.IST.Berkeley.EDU (cm06fe.IST.Berkeley.EDU [169.229.218.147]) } } 9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9RLMq9R025655 } } 9, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 16:22:52 -0500 } } 9, 22 -- Received: from zackg-lap.ssl.berkeley.edu ([128.32.13.191]) } } 9, 22 -- by cm06fe.ist.berkeley.edu with esmtpsa (TLSv1:AES128-SHA:128) } } 9, 22 -- (Exim 4.76) } } 9, 22 -- (auth plain:zackg-at-berkeley.edu) } } 9, 22 -- (envelope-from {zackg-at-berkeley.edu} ) } } 9, 22 -- id 1RJXPX-0002TM-Lj } } 9, 22 -- for Microscopy-at-microscopy.com; Thu, 27 Oct 2011 14:22:52 -0700 } } 9, 22 -- From: Zack Gainsforth {zackg-at-berkeley.edu} } } 9, 22 -- Content-Type: text/plain; charset=us-ascii } } 9, 22 -- Subject: SEM - Reducing carbon deposition } } 9, 22 -- Date: Thu, 27 Oct 2011 14:22:51 -0700 } } 9, 22 -- References: {BF5A6C75-1DAF-4D85-9B60-FF60C3674870-at-ssl.berkeley.edu} } } 9, 22 -- To: Microscopy-at-microscopy.com } } 9, 22 -- Message-Id: {9F0F9A80-7BFA-4FB2-BF4F-0AF025D2821F-at-berkeley.edu} } } 9, 22 -- Mime-Version: 1.0 (Apple Message framework v1251.1) } } 9, 22 -- X-Mailer: Apple Mail (2.1251.1) } } 9, 22 -- Content-Transfer-Encoding: 8bit } } 9, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9RLMq9R025655 } } ==============================End of - Headers============================== } } } }
==============================Original Headers============================== 6, 39 -- From vray-at-partbeamsystech.com Thu Oct 27 18:35:42 2011 6, 39 -- Received: from nm20-vm0.bullet.mail.sp2.yahoo.com (nm20-vm0.bullet.mail.sp2.yahoo.com [98.139.91.218]) 6, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9RNZfWb031213 6, 39 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 18:35:42 -0500 6, 39 -- Received: from [98.139.91.62] by nm20.bullet.mail.sp2.yahoo.com with NNFMP; 27 Oct 2011 23:35:41 -0000 6, 39 -- Received: from [98.139.91.17] by tm2.bullet.mail.sp2.yahoo.com with NNFMP; 27 Oct 2011 23:35:41 -0000 6, 39 -- Received: from [127.0.0.1] by omp1017.mail.sp2.yahoo.com with NNFMP; 27 Oct 2011 23:35:41 -0000 6, 39 -- X-Yahoo-Newman-Id: 463777.46641.bm-at-omp1017.mail.sp2.yahoo.com 6, 39 -- Received: (qmail 57042 invoked from network); 27 Oct 2011 23:35:41 -0000 6, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1319758541; bh=xLuhTKQuFmkfns+mg/FsXxEAgzZHszgIj6TLQR7jQGE=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=TMlrQs9hCqwcwWL37mFOPAH00raK5a4oyycPKWggv/eXEQKWlBLGkB+TJexl2NXhjN7LdbVk4BKCPwCDZk40RfN+Fl6ttnt1/0p5vJwDMSptfj8jRh+3a8gtZ87w4ZbF1xD1bgpcx7WnVbY7zL4Ukk6uFfvnW2haEXda+fXlNmg= 6, 39 -- X-Yahoo-Newman-Property: ymail-3 6, 39 -- X-YMail-OSG: bFN7lYYVM1mnasxl0UwcZSrXPv9kudfX7IsdttvS3dA8y8x 6, 39 -- 8ejxPeErF6KZO_U6FfPPx6RqMy4SZ2kGWpjRr7E1KnV46kb9JKdImfWWc8xG 6, 39 -- NO_w5IhEvnWxXt_yItiaU4HZd7SzW6EKIUgu8r_Z5vLz4K9O3eOmecFoHjoD 6, 39 -- MGMjqRPRC3wVqVHaKPvBTQCZI9pLDQtQm7Hf9zYOxDYUCdw8fGhP9VvKEbny 6, 39 -- nrMEhlBU2QVRGR6oKVodRsk1VT6nj3cl9Yr_8PMq7sNG8nstRo_nOBSHtNO2 6, 39 -- RRtuIBBKw9e5PwmdaDBeY1j8o5GRRmcnFu6lO1Y9dF88EOEEgkr.XHA2GfTn 6, 39 -- 8_icvBr6y6n_3zkzh_S0Mh3UnAFnw9umYVCE1vrr7fQVUklHlrO_LbTkOZlE 6, 39 -- X.hfacVQGBPCiDFsGkPmE2ixE00ZTwIpV2KiwyutVXmz0Byj59kTAXvKP1h_ 6, 39 -- LfPpQN3s2zwZ6nykkldnBcXykr6bzRgSC9CEgYS8WEDPKdN1V1BNYR65ih5s 6, 39 -- zMNa4Fp3P_5kz3SlJ.GaVGwYl3NXBdSDERPdUmqIBuroW.FdN9_mfUpDDsRZ 6, 39 -- F7EjOIiQvCIpUpTOTE7kzlFOY8.h1iAxIW_bMFtSwHrKJOB4IRgXEW8b7meM 6, 39 -- jZCgFiKJBXrpGJNGVnyuIAmUjTDFtL_raT.Wl4ttqIV_dMg-- 6, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 6, 39 -- Received: from [192.168.5.252] (vray-at-64.134.30.249 with plain) 6, 39 -- by smtp115.biz.mail.sp1.yahoo.com with SMTP; 27 Oct 2011 16:35:41 -0700 PDT 6, 39 -- Message-ID: {4EA9EACB.2070901-at-partbeamsystech.com} 6, 39 -- Date: Thu, 27 Oct 2011 19:35:39 -0400 6, 39 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 6, 39 -- Reply-To: vray-at-partbeamsystech.com 6, 39 -- Organization: PBS&T 6, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 6, 39 -- MIME-Version: 1.0 6, 39 -- To: Zack Gainsforth {zackg-at-berkeley.edu} , microscopy-at-microscopy.com 6, 39 -- Subject: Re: [Microscopy] SEM - Reducing carbon deposition 6, 39 -- References: {201110272124.p9RLOMdd026953-at-ns.microscopy.com} {4EA9E257.9080307-at-partbeamsystech.com} {B972B8C6-27E2-4E67-89BB-12BC77752FBA-at-berkeley.edu} 6, 39 -- In-Reply-To: {B972B8C6-27E2-4E67-89BB-12BC77752FBA-at-berkeley.edu} 6, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Greetings, In the recent thread on minimizing carbon contamination in the SEM, a commenter cited a group of water based compounds as being good to use. They were: water-based Aquadag, TorrSeal, Hyson-1C, and Ted Pellas high-conductivity colloidal silver. I tried one of them and the stuff was really syrupy and set quickly so that my small and fussy samples were all but impossible to mount in it. I wonder if the original poster (Valery Ray) or anyone might point me to something like this but that is not too viscous and that sets slowly (actually I'd settle for either). I'd love to get away from sticky tape. Many thanks, Tobias -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Professor /_ / __ /__ \ \ \__ Biology Department / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / ___ / \ \__/ \ ____ Amherst, MA, 01003 www.bio.umass.edu/biology/baskin Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
==============================Original Headers============================== 1, 19 -- From baskin-at-bio.umass.edu Thu Oct 27 21:22:01 2011 1, 19 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19]) 1, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9S2M1wl017369 1, 19 -- for {microscopy-at-microscopy.com} ; Thu, 27 Oct 2011 21:22:01 -0500 1, 19 -- Received: from [10.0.1.8] (ip-159.250.65.28.dsl.crocker.net [159.250.65.28]) 1, 19 -- (authenticated bits=0) 1, 19 -- by marlin.bio.umass.edu (8.14.4/8.14.4) with ESMTP id p9S2LwZw021743 1, 19 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 1, 19 -- Thu, 27 Oct 2011 22:22:00 -0400 (EDT) 1, 19 -- Mime-Version: 1.0 1, 19 -- Message-Id: {p06240539cacfc13e766c-at-[10.0.1.8]} 1, 19 -- Date: Thu, 27 Oct 2011 22:21:58 -0400 1, 19 -- To: microscopy-at-microscopy.com 1, 19 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 1, 19 -- Subject: slow drying clean 'glue' for SEM 1, 19 -- Cc: vray-at-partbeamsystech.com 1, 19 -- Content-Type: text/plain; charset="us-ascii" ; format="flowed" 1, 19 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.2.6 (marlin.bio.umass.edu [128.119.55.19]); Thu, 27 Oct 2011 22:22:00 -0400 (EDT) 1, 19 -- X-Scanned-By: MIMEDefang 2.68 on 128.119.55.19 ==============================End of - Headers==============================
Aquadag is water-based and fairly conductive. If it is too syrupy then it can be diluted with DI water to any consistency. If after dilution dried layer is too thin, then you can re-coat after drying. In diluted condition it handles just like paint.
"Conductive Liquid Silver Paint" or "Colloidal Silver Liquid" from Ted Pella is solvent-based and very conductive, also has almost no binder. It can be gently baked in vacuum oven (if sample allows) and has almost no outgassing after the bake. If it is too syrupy then it can be diluted to any consistency by the "extender" which is also sold by Ted Pella :)
Hysol-1C and TorrSeal (which are in reality the same exact thing, TorrSeal is a re-branded Hysol-1C) are AFAIK all-solid epoxies and thus viscous. They also not conductive.
There is another glue that I worked with, which has quite low outgassing - it is sold by Allied High Tech as "Epoxy Bond 110". Very liquid after mixing and would not thicken at all until heated. The catch is that it requires heat for curing and not conductive.
I would also be very interested to learn if there are other no-outgassing or low-outgassing glues out there...
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/27/2011 10:21 PM, Tobias Baskin wrote: } Greetings, } In the recent thread on minimizing carbon contamination in the SEM, a } commenter cited a group of water based compounds as being good to use. } They were: water-based Aquadag, } TorrSeal, Hyson-1C, and Ted Pellas high-conductivity colloidal silver. I } tried one of them and the stuff was really syrupy and set quickly so } that my small and fussy samples were all but impossible to mount in it. } I wonder if the original poster (Valery Ray) or anyone might point me to } something like this but that is not too viscous and that sets slowly } (actually I'd settle for either). I'd love to get away from sticky tape. } Many thanks, } Tobias
==============================Original Headers============================== 9, 39 -- From vray-at-partbeamsystech.com Fri Oct 28 00:08:51 2011 9, 39 -- Received: from nm4-vm0.bullet.mail.sp2.yahoo.com (nm4-vm0.bullet.mail.sp2.yahoo.com [98.139.91.190]) 9, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9S58oGk003658 9, 39 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 00:08:51 -0500 9, 39 -- Received: from [98.139.91.70] by nm4.bullet.mail.sp2.yahoo.com with NNFMP; 28 Oct 2011 05:08:50 -0000 9, 39 -- Received: from [98.139.91.56] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 28 Oct 2011 05:07:50 -0000 9, 39 -- Received: from [127.0.0.1] by omp1056.mail.sp2.yahoo.com with NNFMP; 28 Oct 2011 05:07:50 -0000 9, 39 -- X-Yahoo-Newman-Id: 424596.96708.bm-at-omp1056.mail.sp2.yahoo.com 9, 39 -- Received: (qmail 79341 invoked from network); 28 Oct 2011 05:07:49 -0000 9, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1319778469; bh=UQh2zRtYv2SOD5n7rTUxtdcKz9ka0SGzu/dq8FTSnIE=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=S5iP6rIQFlPtli24fSk9S7oTiCfsfODZZufrLvzSjR2yLErKjPivdxl1Bc/e8duv4EZ/JHghuVljoS81gnVwYomYdxzsDx217rFL2tibJiFMFvsOsAMLKAfYSloU6/oo3ysbarg0V7P5/1J2DV9o9hLwiW08kfBzHEXcY1jG36U= 9, 39 -- X-Yahoo-Newman-Property: ymail-3 9, 39 -- X-YMail-OSG: CwbsO8gVM1me4ykMkPG5F_BSsElnRuQT4i67mKTZUIPrjEq 9, 39 -- TUzCJiRXQkWFNOrWvhynHiwat7Xo0AmfQO6SHJ6ZcRl7KCdF7Fo3fvwrYIpH 9, 39 -- aCMk.O2MFlej3YOx8l5igxdFkSFLQtswf1XHqYQZBK4GmeO7hSHMD7Ao7Mjn 9, 39 -- tbxmChh_NGg1PiE.44hcQGjFkpgG7K.4.1_.cobte3qA4laAAEobbzCgsnzo 9, 39 -- oHaMUbQ0x3UyDLRyoi_aqZsojuwfuTtayXzHewhrMdS0Qp5NKDZae3n9H.d3 9, 39 -- .0vjlHZ.oEozzfJrS2Co.JoO2Ev6bVUMbVfZq6Nvz8I31cn4diso8G3PXb2y 9, 39 -- UYA4T7zm9Uy7Zz66ZGbfvG9iIYxVkQluVM8nFjtFYnoAiT5dPUhdqVZNdETM 9, 39 -- e64J26kAsIeg30ZSU2psNYtlBzBISKuJCfB3l_PA9_3oJZ3pV2Ub0uD2EqYZ 9, 39 -- 6W9CsL777IZWR66Ciz8Qlc3qmfHI7lBbQ.vNuCPE.Fg_MyB0GUF0N1LCJqZr 9, 39 -- I1B31rNv.FZ2IjGnTsHzE35Br2NV7mp_lv2k0jWYj73EDlB0kb8FxTDn.4w- 9, 39 -- - 9, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 9, 39 -- Received: from [10.0.0.76] (vray-at-173.166.159.252 with plain) 9, 39 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 27 Oct 2011 22:07:49 -0700 PDT 9, 39 -- Message-ID: {4EAA38A5.8030108-at-partbeamsystech.com} 9, 39 -- Date: Fri, 28 Oct 2011 01:07:49 -0400 9, 39 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 9, 39 -- Reply-To: vray-at-partbeamsystech.com 9, 39 -- Organization: PBS&T 9, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 9, 39 -- MIME-Version: 1.0 9, 39 -- To: Tobias Baskin {baskin-at-bio.umass.edu} 9, 39 -- CC: microscopy-at-microscopy.com 9, 39 -- Subject: Re: slow drying clean 'glue' for SEM 9, 39 -- References: {p06240539cacfc13e766c-at-[10.0.1.8]} 9, 39 -- In-Reply-To: {p06240539cacfc13e766c-at-[10.0.1.8]} 9, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The listserver has always been my choice for information and help, so here I go again.
Our lab was sipping coffee and talking about incorporating x-ray fluorescence into the SEM/EDS system. We liked the idea: no charged particle decelerating so no bremsstrahlung, but how do you get an element distribution map without moving the sample?
I understand how the SEM and CRTs move the electron beam in a raster, but it seems to me, you have to move the sample to sweep the collimated x-ray beam across the surface. I can't find anything in the add about that.
Any thoughts?
Have a great week-end...............
Frank
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 13, 25 -- From frank_karl-at-ardl.com Fri Oct 28 09:12:11 2011 13, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9SECB3E006934 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 09:12:11 -0500 13, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 13, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Fri, 28 13, 25 -- Oct 2011 10:12:10 -0400 13, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 13, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 13, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 13, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 13, 25 -- Date: Fri, 28 Oct 2011 10:12:09 -0400 13, 25 -- Subject: x-ray fluorescence element maps 13, 25 -- Thread-Topic: x-ray fluorescence element maps 13, 25 -- Thread-Index: AcyVe5TV9u5cU7KYTHe+jo7qWB1xcQ== 13, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010B0D13BA-at-exchange2k7.ad.ardl.com} 13, 25 -- Accept-Language: en-US 13, 25 -- Content-Language: en-US 13, 25 -- X-MS-Has-Attach: 13, 25 -- X-MS-TNEF-Correlator: 13, 25 -- acceptlanguage: en-US 13, 25 -- Content-Type: text/plain; charset="iso-8859-1" 13, 25 -- MIME-Version: 1.0 13, 25 -- Content-Transfer-Encoding: 8bit 13, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9SECB3E006934 ==============================End of - Headers==============================
Another low vapor-pressure conductive adhesive is Epoxy Technology (Epo-tek) H20E. It is a silver filled heat curing epoxy. If your sample can tolerate being heated to 80C then its great. albeit somewhat expensive. It is a little viscous as it contains a fair amount of silver. We use it consistently in 10^-9 torr at room temperature and also at 10^-11 at cryogenic temperatures.
I have no connection with Epo-tek just a satisfied customer.
-Lyle
-- Lyle Gordon Department of Materials Science and Engineering Northwestern University
On Fri, Oct 28, 2011 at 12:17 AM, {vray-at-partbeamsystech.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Tobias, } } Just for clarity: } } Aquadag is water-based and fairly conductive. If it is too syrupy then } it can be diluted with DI water to any consistency. If after dilution } dried layer is too thin, then you can re-coat after drying. In diluted } condition it handles just like paint. } } "Conductive Liquid Silver Paint" or "Colloidal Silver Liquid" from Ted } Pella is solvent-based and very conductive, also has almost no binder. } It can be gently baked in vacuum oven (if sample allows) and has almost } no outgassing after the bake. If it is too syrupy then it can be diluted } to any consistency by the "extender" which is also sold by Ted Pella :) } } Hysol-1C and TorrSeal (which are in reality the same exact thing, } TorrSeal is a re-branded Hysol-1C) are AFAIK all-solid epoxies and thus } viscous. They also not conductive. } } There is another glue that I worked with, which has quite low outgassing } - it is sold by Allied High Tech as "Epoxy Bond 110". Very liquid after } mixing and would not thicken at all until heated. The catch is that it } requires heat for curing and not conductive. } } I would also be very interested to learn if there are other } no-outgassing or low-outgassing glues out there... } } Cheers :) } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 10/27/2011 10:21 PM, Tobias Baskin wrote: } } Greetings, } } In the recent thread on minimizing carbon contamination in the SEM, a } } commenter cited a group of water based compounds as being good to use. } } They were: water-based Aquadag, } } TorrSeal, Hyson-1C, and Ted Pellas high-conductivity colloidal silver. I } } tried one of them and the stuff was really syrupy and set quickly so } } that my small and fussy samples were all but impossible to mount in it. } } I wonder if the original poster (Valery Ray) or anyone might point me to } } something like this but that is not too viscous and that sets slowly } } (actually I'd settle for either). I'd love to get away from sticky tape. } } Many thanks, } } Tobias } } ==============================Original Headers============================== } 9, 39 -- From vray-at-partbeamsystech.com Fri Oct 28 00:08:51 2011 } 9, 39 -- Received: from nm4-vm0.bullet.mail.sp2.yahoo.com (nm4-vm0.bullet.mail.sp2.yahoo.com [98.139.91.190]) } 9, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id p9S58oGk003658 } 9, 39 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 00:08:51 -0500 } 9, 39 -- Received: from [98.139.91.70] by nm4.bullet.mail.sp2.yahoo.com with NNFMP; 28 Oct 2011 05:08:50 -0000 } 9, 39 -- Received: from [98.139.91.56] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 28 Oct 2011 05:07:50 -0000 } 9, 39 -- Received: from [127.0.0.1] by omp1056.mail.sp2.yahoo.com with NNFMP; 28 Oct 2011 05:07:50 -0000 } 9, 39 -- X-Yahoo-Newman-Id: 424596.96708.bm-at-omp1056.mail.sp2.yahoo.com } 9, 39 -- Received: (qmail 79341 invoked from network); 28 Oct 2011 05:07:49 -0000 } 9, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1319778469; bh=UQh2zRtYv2SOD5n7rTUxtdcKz9ka0SGzu/dq8FTSnIE=; h=X-Yahoo-Newman-Property:X-YMail-OSG:X-Yahoo-SMTP:Received:Message-ID:Date:From:Reply-To:Organization:User-Agent:MIME-Version:To:CC:Subject:References:In-Reply-To:Content-Type:Content-Transfer-Encoding; b=S5iP6rIQFlPtli24fSk9S7oTiCfsfODZZufrLvzSjR2yLErKjPivdxl1Bc/e8duv4EZ/JHghuVljoS81gnVwYomYdxzsDx217rFL2tibJiFMFvsOsAMLKAfYSloU6/oo3ysbarg0V7P5/1J2DV9o9hLwiW08kfBzHEXcY1jG36U= } 9, 39 -- X-Yahoo-Newman-Property: ymail-3 } 9, 39 -- X-YMail-OSG: CwbsO8gVM1me4ykMkPG5F_BSsElnRuQT4i67mKTZUIPrjEq } 9, 39 -- TUzCJiRXQkWFNOrWvhynHiwat7Xo0AmfQO6SHJ6ZcRl7KCdF7Fo3fvwrYIpH } 9, 39 -- aCMk.O2MFlej3YOx8l5igxdFkSFLQtswf1XHqYQZBK4GmeO7hSHMD7Ao7Mjn } 9, 39 -- tbxmChh_NGg1PiE.44hcQGjFkpgG7K.4.1_.cobte3qA4laAAEobbzCgsnzo } 9, 39 -- oHaMUbQ0x3UyDLRyoi_aqZsojuwfuTtayXzHewhrMdS0Qp5NKDZae3n9H.d3 } 9, 39 -- .0vjlHZ.oEozzfJrS2Co.JoO2Ev6bVUMbVfZq6Nvz8I31cn4diso8G3PXb2y } 9, 39 -- UYA4T7zm9Uy7Zz66ZGbfvG9iIYxVkQluVM8nFjtFYnoAiT5dPUhdqVZNdETM } 9, 39 -- e64J26kAsIeg30ZSU2psNYtlBzBISKuJCfB3l_PA9_3oJZ3pV2Ub0uD2EqYZ } 9, 39 -- 6W9CsL777IZWR66Ciz8Qlc3qmfHI7lBbQ.vNuCPE.Fg_MyB0GUF0N1LCJqZr } 9, 39 -- I1B31rNv.FZ2IjGnTsHzE35Br2NV7mp_lv2k0jWYj73EDlB0kb8FxTDn.4w- } 9, 39 -- - } 9, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- } 9, 39 -- Received: from [10.0.0.76] (vray-at-173.166.159.252 with plain) } 9, 39 -- by smtp115.biz.mail.mud.yahoo.com with SMTP; 27 Oct 2011 22:07:49 -0700 PDT } 9, 39 -- Message-ID: {4EAA38A5.8030108-at-partbeamsystech.com} } 9, 39 -- Date: Fri, 28 Oct 2011 01:07:49 -0400 } 9, 39 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} } 9, 39 -- Reply-To: vray-at-partbeamsystech.com } 9, 39 -- Organization: PBS&T } 9, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 Thunderbird/7.0.1 } 9, 39 -- MIME-Version: 1.0 } 9, 39 -- To: Tobias Baskin {baskin-at-bio.umass.edu} } 9, 39 -- CC: microscopy-at-microscopy.com } 9, 39 -- Subject: Re: slow drying clean 'glue' for SEM } 9, 39 -- References: {p06240539cacfc13e766c-at-[10.0.1.8]} } 9, 39 -- In-Reply-To: {p06240539cacfc13e766c-at-[10.0.1.8]} } 9, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 39 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 10, 28 -- From lgordon-at-gmail.com Fri Oct 28 09:51:51 2011 10, 28 -- Received: from mail-fx0-f41.google.com (mail-fx0-f41.google.com [209.85.161.41]) 10, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9SEpowJ024377 10, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 09:51:51 -0500 10, 28 -- Received: by faat2 with SMTP id t2so1315523faa.0 10, 28 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 07:51:50 -0700 (PDT) 10, 28 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 28 -- d=gmail.com; s=gamma; 10, 28 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 10, 28 -- :content-type:content-transfer-encoding; 10, 28 -- bh=LaY1bUG/EsTk9GpM04CvKhgitIHSOSVi2067Hpdrn4w=; 10, 28 -- b=vHnmLZMsfKbZ7oQZGjbKioojkZR2ng9L+Jij9Zpwf05QfZ8wvHHz2b8kAgJQQeDHSA 10, 28 -- 994tGWaWQPR5+5hvk98uxIGp7NlT4rXbbeZWuc8YYWg5Hz9UoTfDr6+NKUvqnVmcohBu 10, 28 -- y7Qrsh7Q3zXiKit2k0mwCSu9OR9tp4sIL595I= 10, 28 -- Received: by 10.223.39.20 with SMTP id d20mr6453213fae.37.1319813509370; Fri, 10, 28 -- 28 Oct 2011 07:51:49 -0700 (PDT) 10, 28 -- MIME-Version: 1.0 10, 28 -- Received: by 10.152.24.229 with HTTP; Fri, 28 Oct 2011 07:51:28 -0700 (PDT) 10, 28 -- In-Reply-To: {201110280517.p9S5HSMC014787-at-ns.microscopy.com} 10, 28 -- References: {201110280517.p9S5HSMC014787-at-ns.microscopy.com} 10, 28 -- From: Lyle Gordon {lgordon-at-gmail.com} 10, 28 -- Date: Fri, 28 Oct 2011 09:51:28 -0500 10, 28 -- Message-ID: {CAFhTNAnff9WDPX2Kf4ODjFjW1XKx=9fs6SNBcT=5FWL7MpZAOA-at-mail.gmail.com} 10, 28 -- Subject: Re: [Microscopy] Re: slow drying clean 'glue' for SEM 10, 28 -- To: vray-at-partbeamsystech.com, microscopy-at-microscopy.com 10, 28 -- Content-Type: text/plain; charset=ISO-8859-1 10, 28 -- Content-Transfer-Encoding: 8bit 10, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9SEpowJ024377 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tbogea-at-mail.ubc.ca Name: Tami Bogea
Organization: University of British Columbia
Title-Subject: [Filtered] Immuno EM
Message: Hi everyone
Once again I turn to you for advice. I am working on a immuno-electron microscopy protocol to study the localization of rhodopsin mutants in frog photoreceptors. Currently, I am using a post-embedding method where the fixatives are made with phosphate buffer and the stains include 2% aqueous uranyl acetate and Venables & Coggleshall's lead citrate. The labelling looks fine but the sections show a lot of precipitate in the background. I would be pleased to hear your comments and suggestions on how to minimize these precipitates. Would sodium cacodylate buffer be an option in this case? Thanks a bunch!
Tami Bogea, PhD Research Associate -- Ophthalmology & Visual Sciences University of British Columbia Login Host: 137.82.183.94 ---------------------------------------------------------------------------
==============================Original Headers============================== 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Oct 28 19:41:25 2011 9, 25 -- Received: from znl.com ([206.69.208.20]) 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9T0fPBv017530 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 19:41:25 -0500 9, 25 -- Received: from localhost (localhost [127.0.0.1]) 9, 25 -- by znl.com (Postfix) with ESMTP id B5D3943309E 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 19:41:25 -0500 (CDT) 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 9, 25 -- Received: from znl.com ([127.0.0.1]) 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 9, 25 -- with ESMTP id afFA39YVl68I for {microscopy-at-microscopy.com} ; 9, 25 -- Fri, 28 Oct 2011 19:41:23 -0500 (CDT) 9, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 9, 25 -- by znl.com (Postfix) with ESMTPA id 0CB90433093 9, 25 -- for {microscopy-at-microscopy.com} ; Fri, 28 Oct 2011 19:41:23 -0500 (CDT) 9, 25 -- Message-ID: {4EAB4BB3.6060602-at-microscopy.com} 9, 25 -- Date: Fri, 28 Oct 2011 19:41:23 -0500 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 9, 25 -- Reply-To: tbogea-at-mail.ubc.ca 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 9, 25 -- MIME-Version: 1.0 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 9, 25 -- Subject: viaWWW:Immuno EM 9, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hello all, We recently have noticed a problem on our Phillips CM 120 TEM when imaging 12nm nanogold particles. At high magnifications (65,000 x) we see small image tails coming off of the gold particles. They are always in the same direction (diagonal pointing to about 7 o`clock). We have tried tilting the grid, corrected for astigmatism, higher Kv and smaller condenser, objective and final apertures. We can minimize it but it still remains. The conjugated gold is less than a year old, this is for post embed IEM labeling. Any thoughts?
Thanks in advance for any clues.
Michael Delannoy
==============================Original Headers============================== 3, 29 -- From prvs=276c03497=delannoy-at-jhmi.edu Sat Oct 29 09:49:42 2011 3, 29 -- Received: from ipex4.johnshopkins.edu (ipex4.johnshopkins.edu [128.220.161.141]) 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9TEng4l010720 3, 29 -- for {microscopy-at-microscopy.com} ; Sat, 29 Oct 2011 09:49:42 -0500 3, 29 -- X-IronPort-AV: E=Sophos;i="4.69,424,1315195200"; 3, 29 -- d="scan'208";a="111693597" 3, 29 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 3, 29 -- by ipex4.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 29 Oct 2011 10:49:42 -0400 3, 29 -- MIME-version: 1.0 3, 29 -- Content-transfer-encoding: 7BIT 3, 29 -- Content-disposition: inline 3, 29 -- Content-type: text/plain; CHARSET=US-ASCII 3, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 3, 29 -- by commsmail.johnshopkins.edu 3, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 3, 29 -- with ESMTP id {0LTU002X716TOND0-at-commsmail.johnshopkins.edu} for 3, 29 -- microscopy-at-microscopy.com; Sat, 29 Oct 2011 10:49:41 -0400 (EDT) 3, 29 -- Received: from [10.173.109.14] (Forwarded-For: [98.233.173.11]) 3, 29 -- by commsmail.johnshopkins.edu (mshttpd); Sat, 29 Oct 2011 10:49:41 -0400 3, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} 3, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 29 -- Message-id: {7180fee68959.4eabda45-at-johnshopkins.edu} 3, 29 -- Date: Sat, 29 Oct 2011 10:49:41 -0400 3, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 3, 29 -- 2009) 3, 29 -- Content-language: en 3, 29 -- Subject: gold tails 3, 29 -- X-Accept-Language: en 3, 29 -- Priority: normal ==============================End of - Headers==============================
What you are describing sounds like a "coma" aberration. This arises from your beam being off the optic axis when it goes through the objective lens. Look to see if you have an image shift as you change focus. This is a dead give-away that your beam centering is off. The solution is to do a rotation center.
With the sample at the eucentric height, focus the image. Set the tilt pivot points Center the illumination. Focus the beam with C2 and select Algn | Pivot Point X.Use the MF knobs to superimpose the spots. Repeat with Algn| Pivot Point Y. Rotation Center Focus the beam at 60-100kX and center the illumination. Select Algn| Rot Center and use the MF knobs to set the wobble point to the center.The focus step will adjust the wobble amplitude.
I hope this helps.
Cheers, Henk
At 10/29/2011 10:50 AM, delannoy-at-jhmi.edu wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } We recently have noticed a problem on our Phillips CM 120 TEM when imaging 12nm nanogold particles. At high magnifications (65,000 x) we see small image tails coming off of the gold particles. They are always in the same direction (diagonal pointing to about 7 o`clock). We have tried tilting the grid, corrected for astigmatism, higher Kv and smaller condenser, objective and final apertures. We can minimize it but it still remains. The conjugated gold is less than a year old, this is for post embed IEM labeling. Any thoughts? } } Thanks in advance for any clues. } } Michael Delannoy } } ==============================Original Headers============================== } 3, 29 -- From prvs=276c03497=delannoy-at-jhmi.edu Sat Oct 29 09:49:42 2011 } 3, 29 -- Received: from ipex4.johnshopkins.edu (ipex4.johnshopkins.edu [128.220.161.141]) } 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9TEng4l010720 } 3, 29 -- for {microscopy-at-microscopy.com} ; Sat, 29 Oct 2011 09:49:42 -0500 } 3, 29 -- X-IronPort-AV: E=Sophos;i="4.69,424,1315195200"; } 3, 29 -- d="scan'208";a="111693597" } 3, 29 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) } 3, 29 -- by ipex4.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 29 Oct 2011 10:49:42 -0400 } 3, 29 -- MIME-version: 1.0 } 3, 29 -- Content-transfer-encoding: 7BIT } 3, 29 -- Content-disposition: inline } 3, 29 -- Content-type: text/plain; CHARSET=US-ASCII } 3, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) } 3, 29 -- by commsmail.johnshopkins.edu } 3, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) } 3, 29 -- with ESMTP id {0LTU002X716TOND0-at-commsmail.johnshopkins.edu} for } 3, 29 -- microscopy-at-microscopy.com; Sat, 29 Oct 2011 10:49:41 -0400 (EDT) } 3, 29 -- Received: from [10.173.109.14] (Forwarded-For: [98.233.173.11]) } 3, 29 -- by commsmail.johnshopkins.edu (mshttpd); Sat, 29 Oct 2011 10:49:41 -0400 } 3, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} } 3, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 3, 29 -- Message-id: {7180fee68959.4eabda45-at-johnshopkins.edu} } 3, 29 -- Date: Sat, 29 Oct 2011 10:49:41 -0400 } 3, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 } 3, 29 -- 2009) } 3, 29 -- Content-language: en } 3, 29 -- Subject: gold tails } 3, 29 -- X-Accept-Language: en } 3, 29 -- Priority: normal } ==============================End of - Headers============================== }
-- Hendrik O. Colijn www.ceof.ohio-state.edu OSU Campus Electron Optics Facility colijn.1-at-osu.edu 040 Fontana Labs (614) 292-0674 (V) 116 W. 19th Ave. (614) 292-7523 (F) Columbus, OH 43210
"Time is that quality of nature which keeps things from happening all at once. Lately it doesn't seem to be working."
==============================Original Headers============================== 10, 27 -- From colijn.1-at-osu.edu Sat Oct 29 12:34:31 2011 10, 27 -- Received: from ER6S1.ECR6.OHIO-STATE.EDU (er6s1.ecr6.ohio-state.edu [164.107.76.2]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9THYVKF003453 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 29 Oct 2011 12:34:31 -0500 10, 27 -- Received: from CONVERSION-DAEMON.er6s1.ecr6.ohio-state.edu by 10, 27 -- er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 27 -- id {01O7SHYLKPQ893LU4D-at-ecr6.ohio-state.edu} for microscopy-at-microscopy.com; 10, 27 -- Sat, 29 Oct 2011 13:34:31 -0400 (EDT) 10, 27 -- Received: from [192.168.1.103] 10, 27 -- (d118-75-116-26.try.wideopenwest.com [75.118.26.116]) 10, 27 -- by er6s1.ecr6.ohio-state.edu (PMDF V6.5-x2 #31794) 10, 27 -- with ESMTPA id {01O7SHYL2272937XIB-at-ecr6.ohio-state.edu} ; Sat, 10, 27 -- 29 Oct 2011 13:34:30 -0400 (EDT) 10, 27 -- Date: Sat, 29 Oct 2011 13:34:29 -0400 10, 27 -- From: "Hendrik O. Colijn" {colijn.1-at-osu.edu} 10, 27 -- Subject: Re: [Microscopy] gold tails 10, 27 -- In-reply-to: {201110291450.p9TEovOW012551-at-ns.microscopy.com} 10, 27 -- Sender: colijn-at-er6s1.ecr6.ohio-state.edu 10, 27 -- To: delannoy-at-jhmi.edu, microscopy-at-microscopy.com 10, 27 -- Message-id: {4EAC3925.6090802-at-osu.edu} 10, 27 -- MIME-version: 1.0 10, 27 -- Content-type: text/plain; charset=ISO-8859-1; format=flowed 10, 27 -- Content-transfer-encoding: 7bit 10, 27 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:7.0.1) Gecko/20110929 10, 27 -- Thunderbird/7.0.1 10, 27 -- X-Env-From: auth/colijn.1-at-osu.edu 10, 27 -- References: {201110291450.p9TEovOW012551-at-ns.microscopy.com} ==============================End of - Headers==============================
Dear Michael These tails are they physical smaller particles or just a contrast change in the image? If it is a contrast change then do a complete re-allighnment. Might be a aberration.
Stephan
On Sat, Oct 29, 2011 at 4:56 PM, {delannoy-at-jhmi.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all, } We recently have noticed a problem on our Phillips CM 120 TEM when imaging 12nm nanogold particles. At high magnifications (65,000 x) we see small image tails coming off of the gold particles. They are always in the same direction (diagonal pointing to about 7 o`clock). We have tried tilting the grid, corrected for astigmatism, higher Kv and smaller condenser, objective and final apertures. We can minimize it but it still remains. The conjugated gold is less than a year old, this is for post embed IEM labeling. Any thoughts? } } Thanks in advance for any clues. } } Michael Delannoy } } ==============================Original Headers============================== } 3, 29 -- From prvs=276c03497=delannoy-at-jhmi.edu Sat Oct 29 09:49:42 2011 } 3, 29 -- Received: from ipex4.johnshopkins.edu (ipex4.johnshopkins.edu [128.220.161.141]) } 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9TEng4l010720 } 3, 29 -- for {microscopy-at-microscopy.com} ; Sat, 29 Oct 2011 09:49:42 -0500 } 3, 29 -- X-IronPort-AV: E=Sophos;i="4.69,424,1315195200"; } 3, 29 -- d="scan'208";a="111693597" } 3, 29 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) } 3, 29 -- by ipex4.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 29 Oct 2011 10:49:42 -0400 } 3, 29 -- MIME-version: 1.0 } 3, 29 -- Content-transfer-encoding: 7BIT } 3, 29 -- Content-disposition: inline } 3, 29 -- Content-type: text/plain; CHARSET=US-ASCII } 3, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) } 3, 29 -- by commsmail.johnshopkins.edu } 3, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) } 3, 29 -- with ESMTP id {0LTU002X716TOND0-at-commsmail.johnshopkins.edu} for } 3, 29 -- microscopy-at-microscopy.com; Sat, 29 Oct 2011 10:49:41 -0400 (EDT) } 3, 29 -- Received: from [10.173.109.14] (Forwarded-For: [98.233.173.11]) } 3, 29 -- by commsmail.johnshopkins.edu (mshttpd); Sat, 29 Oct 2011 10:49:41 -0400 } 3, 29 -- From: Michael J Delannoy {delannoy-at-jhmi.edu} } 3, 29 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 3, 29 -- Message-id: {7180fee68959.4eabda45-at-johnshopkins.edu} } 3, 29 -- Date: Sat, 29 Oct 2011 10:49:41 -0400 } 3, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 } 3, 29 -- 2009) } 3, 29 -- Content-language: en } 3, 29 -- Subject: gold tails } 3, 29 -- X-Accept-Language: en } 3, 29 -- Priority: normal } ==============================End of - Headers============================== }
-- Stephan H Coetzee EM Scientist Electron Microscope Unit University of Botswana
==============================Original Headers============================== 7, 29 -- From s.h.coetzee-at-gmail.com Sat Oct 29 15:21:12 2011 7, 29 -- Received: from mail-iy0-f169.google.com (mail-iy0-f169.google.com [209.85.210.169]) 7, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9TKLCTu011249 7, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 29 Oct 2011 15:21:12 -0500 7, 29 -- Received: by iagf6 with SMTP id f6so7265122iag.0 7, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 29 Oct 2011 13:21:12 -0700 (PDT) 7, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 29 -- d=gmail.com; s=gamma; 7, 29 -- h=mime-version:in-reply-to:references:date:message-id:subject:from:to 7, 29 -- :cc:content-type:content-transfer-encoding; 7, 29 -- bh=tjKkrMpHNJTDBfdQIPesKpXndTAvxr//3wFXKd+JOX0=; 7, 29 -- b=nA64268pJxvlfX1uT6O1u8NwcmxilpBrlNfoRKLYJVItOViyJi+Z1zEesELiL8x8ZV 7, 29 -- f9bDhI5IAaP048fkriGQ77dzCjBabZK0Q/RbBUPTJdPvH7GEgwDHKZAd0VwcV71MrUI+ 7, 29 -- 7Z91Vw3/uvC54P2as2bvOPC4ftuIhucOKsZrA= 7, 29 -- MIME-Version: 1.0 7, 29 -- Received: by 10.68.12.138 with SMTP id y10mr12266519pbb.70.1319919671099; Sat, 7, 29 -- 29 Oct 2011 13:21:11 -0700 (PDT) 7, 29 -- Received: by 10.142.214.9 with HTTP; Sat, 29 Oct 2011 13:21:11 -0700 (PDT) 7, 29 -- In-Reply-To: {201110291456.p9TEubQk019822-at-ns.microscopy.com} 7, 29 -- References: {201110291456.p9TEubQk019822-at-ns.microscopy.com} 7, 29 -- Date: Sat, 29 Oct 2011 22:21:11 +0200 7, 29 -- Message-ID: {CALK339Vqc7YGg-LpP1Xx1vFNoGmGTKx_Jo9mV2_WXAc56r+4Bw-at-mail.gmail.com} 7, 29 -- Subject: Re: [Microscopy] gold tails 7, 29 -- From: Stephan Coetzee {s.h.coetzee-at-gmail.com} 7, 29 -- To: delannoy-at-jhmi.edu 7, 29 -- Cc: microscopy {Microscopy-at-microscopy.com} 7, 29 -- Content-Type: text/plain; charset=ISO-8859-1 7, 29 -- Content-Transfer-Encoding: 8bit 7, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id p9TKLCTu011249 ==============================End of - Headers==============================
Dear colleagues - please note the symposium on "Local probing techniques and in-situ measurements in materials science (CCC)" at the MRS Spring meeting, with submission deadline tomorrow.
Sergei Kalinin
CCC. Local probing techniques and in-situ measurements in material sciences
The grand challenges of the clean energy future require new breakthroughs in materials and systems, in which precision measurement techniques, particularly nondestructive, real time, in situ, and local probing techniques, will play a critical role. Those new emerging characterization techniques will help reveal the microscopic mechanisms underpinning the performance and life time of energy storage and conversion systems.
This symposium is devoted to disseminating original research in applying local probing techniques and in situ measurements to materials discovery in general, , and to energy systems optimization, safety analysis, failure diagnosis, and lifetime prediction, in particular. This symposium will showcase the revelation of structures and properties of materials using in situ scanning probe, microscopy, spectroscopy, tomography, scattering, activation, and radiation measurements.
It is the goal of this symposium to bring together experts from materials science, advanced characterization techniques, theoretical community, and industry interested in the development of experimental techniques capable of addressing fundamental mechanisms in materials synthesis, process and applications. In addition to providing a platform for discussing state-of-the-art local and in-situ characterization methods, this symposium will help in formulating the outstanding research needs, grand challenges, applications, and development pathway for this rapidly emerging field.
We would like to encourage you to consider submitting an abstract to this symposium.
Kind regards,
Nina Balke Oak Ridge National Laboratory balken-at-ornl.gov
Howard Wang State University of New York, Binghamton wangh-at-binghamton.edu
Job Rijssenbeek GE Global Research rijssenb-at-research.ge.com
Thilo Glatzel University of Basel Thilo.glatzel-at-unibas.ch
-- Sergei V. Kalinin co-Theme Leader for Functional Imaging on the Nanoscale The Center for Nanophase Materials Sciences and Materials Sciences and Technology Division Oak Ridge National Laboratory Oak Ridge, TN 37922
Adjunct Associate Professor, Department of Materials Science and Engineering, University of Tennessee, Knoxville
Phone: (865) 241-0236 http://imaging.ornl.gov
==============================Original Headers============================== 19, 26 -- From sergei2-at-ornl.gov Mon Oct 31 11:53:01 2011 19, 26 -- Received: from emroute3.ornl.gov (emroute3.ornl.gov [160.91.4.110]) 19, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id p9VGr0UO021361 19, 26 -- for {microscopy-at-microscopy.com} ; Mon, 31 Oct 2011 11:53:01 -0500 19, 26 -- Received: from emroute3.ornl.gov ([127.0.0.1]) 19, 26 -- by emroute3.ornl.gov (PMDF V6.5-x6 #31890) 19, 26 -- with ESMTP id {0LTX00808W8CW4-at-emroute3.ornl.gov} for 19, 26 -- microscopy-at-microscopy.com; Mon, 31 Oct 2011 12:53:00 -0400 (EDT) 19, 26 -- Received: from CONVERSION-DAEMON.emroute3.ornl.gov by emroute3.ornl.gov 19, 26 -- (PMDF V6.5-x6 #31890) id {0LTX00801W8BWA-at-emroute3.ornl.gov} for 19, 26 -- microscopy-at-microscopy.com; Mon, 31 Oct 2011 12:53:00 -0400 (EDT) 19, 26 -- Received: from [128.219.192.60] (sergei2.ornl.gov [128.219.192.60]) 19, 26 -- by emroute3.ornl.gov (PMDF V6.5-x6 #31890) 19, 26 -- with ESMTPS id {0LTX0081JW8BUX-at-emroute3.ornl.gov} for 19, 26 -- microscopy-at-microscopy.com; Mon, 31 Oct 2011 12:52:59 -0400 (EDT) 19, 26 -- Date: Mon, 31 Oct 2011 12:52:59 -0400 19, 26 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 19, 26 -- Subject: MRS Spring 2012 - Local probing techniques and in-situ measurements in 19, 26 -- material sciences 19, 26 -- To: microscopy-at-microscopy.com 19, 26 -- Message-id: {4EAED26B.3060805-at-ornl.gov} 19, 26 -- MIME-version: 1.0 19, 26 -- Content-type: text/plain; format=flowed; charset=ISO-8859-1 19, 26 -- Content-transfer-encoding: 7bit 19, 26 -- User-Agent: Mozilla/5.0 (Windows; U; Windows NT 5.1; en-US; rv:1.9.2.23) 19, 26 -- Gecko/20110920 Lightning/1.0b2 Thunderbird/3.1.15 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman-at-pacific.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman-at-pacific.edu Name: Barbara Plowman
Organization: University of the Pacific-Arthur A. Dugoni School of Dentistry Title-Subject: [Filtered] Corresponding mags for taps on Philips 200
Message: Does anybody have the magnifications that correspond to the tap levels on the Philips 200 TEM? I have some old negatives I've been looking through and the log books list the tap number instead of the magnifications. We sent that TEM to Canada in 1994. Thank you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mjones96-at-jhmi.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Dear All, We bought an ancient Leica Frigocut 2800 N from eBay and the specimen is clamped on to the microtome by a twist and lock mechanism. This old thing is so worn, that twist does not lead to a perfect lock and that little movement spells disaster for sectioning. I think I could replace the face of this (the "specimen clamp") with something newer that might be more stable if it's less worn. Anyone got parts laying around for any Leica model that's that old? It's held on by allen screws so i think i can switch it out if i had another one. Any ideas? Leica's no help...
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both blauman-at-american.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: blauman-at-american.edu Name: Sara Blauman
Organization: American University
Title-Subject: [Filtered] TEM charge on tissue sections
Message: I am trying to obtain cross sections of 1 mm square pieces of brain tissue that are 50 microns thick. I tried flat embedding the specimen, polymerizing, and then slipping the flat block inside a BEEM capsule and filling it with resin and polymerizing again. I am doing this to try to get the specimen so that it is not at the edge of the section since the sections sink to the bottom in the flat mold. So there is a boundary between the two resins with the specimen at the boundary. The resins were different batches and there is a difference in their color and the way they cut when seen under magnification.
I hand trim with a razor blade, cut 700 nm thick sections, and finally 70 nm thin sections. All goes well until I try to manipulate the thin sections in the boat. They stick to my eyelash tools by wrapping around the eyelash every time I try to touch them. After about a minute after I stop cutting, all the sections in the ribbon detach from each other and assume positions equidistant from each other. Attempting to move the sections closer in order to pick them up in the loop is like trying to move the north ends of two bar magnets toward each other. So all I am getting in my loop is one section!
I thought that I read somewhere to put a drop of ethanol in the boat to eliminate static charge and I tried this to no avail. I have also tried sectioning other "normal" blocks and I do not have the problem with them.
Does anyone know how to remove the charge from the sections? Also, if anyone has any tips for cross sectioning tissue I would be most appreciative.
Thank you, Sara Blauman American University Login Host: 147.9.36.73 ---------------------------------------------------------------------------
==============================Original Headers============================== 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Nov 1 19:31:03 2011 11, 25 -- Received: from znl.com ([206.69.208.20]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA20V33q005757 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 1 Nov 2011 19:31:03 -0500 11, 25 -- Received: from localhost (localhost [127.0.0.1]) 11, 25 -- by znl.com (Postfix) with ESMTP id 046DE436058 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 1 Nov 2011 19:31:03 -0500 (CDT) 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 25 -- Received: from znl.com ([127.0.0.1]) 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 25 -- with ESMTP id O5UBE+qFrtVb for {microscopy-at-microscopy.com} ; 11, 25 -- Tue, 1 Nov 2011 19:31:00 -0500 (CDT) 11, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 11, 25 -- by znl.com (Postfix) with ESMTPA id 6072C43604D 11, 25 -- for {microscopy-at-microscopy.com} ; Tue, 1 Nov 2011 19:31:00 -0500 (CDT) 11, 25 -- Message-ID: {4EB08F44.3040803-at-microscopy.com} 11, 25 -- Date: Tue, 01 Nov 2011 19:31:00 -0500 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 25 -- Reply-To: blauman-at-american.edu 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 25 -- MIME-Version: 1.0 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 25 -- Subject: viaWWW:TEM charging on tissue sections 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yuhong.wu-at-solvay.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: yuhong.wu-at-solvay.com Name: Yuhong Wu
Organization: Solvay Advanced Polymers
Title-Subject: [Filtered] Zeiss Axioplan Service Company near Atlanta
Message: Dear All, we have an old Zeiss Axioplan, which is no longer serviced by Zeiss. We are looking for a local service company that can do cleaning and repairs. Thanks a lot in advance!
I enjoy very much the present time, when engineering, physics and mathemics have come to a maturity which allows all kinds of new microscopy techniques to be developed.
I came across an interesting article in PNAS: http://www.pnas.org/content/early/2011/09/26/1110681108.full.pdf
I think it is interesting for life and material scientists. As a biologist I don't understand all the technological intricacies of the method but I wonder if, given the simple material they used to get this kind of pictures, the resolution couldn't be further improved by using more sophisticated material (smaller CMOS pixel size for example?).
No personal interest, just an impressed reader ;-)
Stephane
==============================Original Headers============================== 3, 37 -- From nizets2-at-yahoo.com Wed Nov 2 09:51:46 2011 3, 37 -- Received: from nm5.bullet.mail.sp2.yahoo.com (nm5.bullet.mail.sp2.yahoo.com [98.139.91.75]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pA2EpjtZ023808 3, 37 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2011 09:51:46 -0500 3, 37 -- Received: from [98.139.91.70] by nm5.bullet.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- Received: from [98.139.91.54] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- Received: from [127.0.0.1] by omp1054.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- X-Yahoo-Newman-Property: ymail-3 3, 37 -- X-Yahoo-Newman-Id: 687278.77456.bm-at-omp1054.mail.sp2.yahoo.com 3, 37 -- Received: (qmail 47852 invoked by uid 60001); 2 Nov 2011 14:51:45 -0000 3, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1320245505; bh=yabWDXlfrIwzg4acn5iDHEzVZwc1/owU33LgFNt6Vls=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=EdAPzz2sy02QW6ivyj5oYV33faY4w1xteyUunrmJ7F/RYcEU3f/oR5NLO4uvyikt/Uik/sxlT8RY/+mZMMPiCMl1BItEDu3MeWSPNdWBVPUr/Q0ew4eVDNJC1SSUccxrVnbmK3CE+lKkNZPFTNlE1Esltpcqqatwq1J/gZQHo78= 3, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 37 -- s=s1024; d=yahoo.com; 3, 37 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 3, 37 -- b=Ii7uwhS07lb3HuzScExxxVMcTOq859gys91dExnVRenMtYSupDei5ihokXwfy1NUZr2kEF3r/tJeyI6vqhQ95F7mtQ/4TwFofp/rYrICK13esLiy23/EnTyaSfIi0ldDDJf5cyEgw7kzcFXT12cE0Nqh2/PBimda0nxiDSnvC7Q=; 3, 37 -- X-YMail-OSG: ciwWW8sVM1m2_Q8wB9V3DePWv0jjFYi7DffZH_N4UaGs_8z 3, 37 -- yTzEbCzbijLvqGa4yGUjm21AhjapOopKDQybzEq5BsmVjUC_Dcm6rH66pG34 3, 37 -- t70ARdpEaIPLF_VByVuyfdBOkCnagU.6gsrNH2ULEKASSnHA5PgZ9yiAevA. 3, 37 -- Juv.9FacS4NQaa.iU.A5V.02e4HDL8tW_NOwVNQ0lOPIHPE1UUHaWz1zJEd5 3, 37 -- xkCPMobF0dY9slTVQFD9kWmjKzUWkx3kKoJhWRY0edrogE5QmqWMnyaTBCfz 3, 37 -- YAOYoD1qyfctPJQUjPoY4y8wMXLsl9ZIuuAnI2iKowy9e2QM12UECsz6Th2G 3, 37 -- wf04SD1T.16Y3NZWVJeKPretPE8WTaIX1GGqVvkaUZu.IEd9TZpTked9azPP 3, 37 -- UYE7CGG_5pz2CDFbdjqfT9MBI8PSMQxbDZVz_fiTnAOLWcMcJbzRf4uEoh44 3, 37 -- UzkP0CfN.FOagdpiCMHj.RxoiZ_fbQin5PKRYGwS_G4_GGoQeaIEU6fKikxw 3, 37 -- CKNq.FnCM90BMcIY7kJDOYcYF2NsNbdbSms0LJ60mXtYghrVk5cZUiA-- 3, 37 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Wed, 02 Nov 2011 07:51:45 PDT 3, 37 -- X-Mailer: YahooMailWebService/0.8.115.325013 3, 37 -- Message-ID: {1320245505.71843.YahooMailNeo-at-web110807.mail.gq1.yahoo.com} 3, 37 -- Date: Wed, 2 Nov 2011 07:51:45 -0700 (PDT) 3, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 37 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 37 -- Subject: ePetri dish: your opinion? 3, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 37 -- MIME-Version: 1.0 3, 37 -- Content-Type: text/plain; charset=iso-8859-1 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA2EpjtZ023808 ==============================End of - Headers==============================
At the risk of exposing my own ignorance let me ask the group: Doesn't the use of computer processing of several low resolution images to make a "new" high resolution images trouble you? Would not/could not a artifact be manipulated (un-intentionally) into something that sends the researcher down blind alleys? I suppose this question has been asked shortly after Fox invented photography and we wondered if it would replace camera lucida drawings.
My TEM camera is the pits, so I "smooth" the image. I can see the pixal change at the edge of the particles, but I'm just measuring the particle diameter and the size categories are big enough. But I would be very uncomfortable looking at structure followed by evaluation fine structure or image information on a highly processed image.
So, am I out in left field with my concerns?
stay safe..............................
Frank
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Wednesday, November 02, 2011 10:01 AM To: Frank Karl
Dear colleagues,
I enjoy very much the present time, when engineering, physics and mathemics have come to a maturity which allows all kinds of new microscopy techniques to be developed.
I came across an interesting article in PNAS: http://www.pnas.org/content/early/2011/09/26/1110681108.full.pdf
I think it is interesting for life and material scientists. As a biologist I don't understand all the technological intricacies of the method but I wonder if, given the simple material they used to get this kind of pictures, the resolution couldn't be further improved by using more sophisticated material (smaller CMOS pixel size for example?).
No personal interest, just an impressed reader ;-)
Stephane
==============================Original Headers============================== 3, 37 -- From nizets2-at-yahoo.com Wed Nov 2 09:51:46 2011 3, 37 -- Received: from nm5.bullet.mail.sp2.yahoo.com (nm5.bullet.mail.sp2.yahoo.com [98.139.91.75]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pA2EpjtZ023808 3, 37 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2011 09:51:46 -0500 3, 37 -- Received: from [98.139.91.70] by nm5.bullet.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- Received: from [98.139.91.54] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- Received: from [127.0.0.1] by omp1054.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- X-Yahoo-Newman-Property: ymail-3 3, 37 -- X-Yahoo-Newman-Id: 687278.77456.bm-at-omp1054.mail.sp2.yahoo.com 3, 37 -- Received: (qmail 47852 invoked by uid 60001); 2 Nov 2011 14:51:45 -0000 3, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1320245505; bh=yabWDXlfrIwzg4acn5iDHEzVZwc1/owU33LgFNt6Vls=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=EdAPzz2sy02QW6ivyj5oYV33faY4w1xteyUunrmJ7F/RYcEU3f/oR5NLO4uvyikt/Uik/sxlT8RY/+mZMMPiCMl1BItEDu3MeWSPNdWBVPUr/Q0ew4eVDNJC1SSUccxrVnbmK3CE+lKkNZPFTNlE1Esltpcqqatwq1J/gZQHo78= 3, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 37 -- s=s1024; d=yahoo.com; 3, 37 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 3, 37 -- b=Ii7uwhS07lb3HuzScExxxVMcTOq859gys91dExnVRenMtYSupDei5ihokXwfy1NUZr2kEF3r/tJeyI6vqhQ95F7mtQ/4TwFofp/rYrICK13esLiy23/EnTyaSfIi0ldDDJf5cyEgw7kzcFXT12cE0Nqh2/PBimda0nxiDSnvC7Q=; 3, 37 -- X-YMail-OSG: ciwWW8sVM1m2_Q8wB9V3DePWv0jjFYi7DffZH_N4UaGs_8z 3, 37 -- yTzEbCzbijLvqGa4yGUjm21AhjapOopKDQybzEq5BsmVjUC_Dcm6rH66pG34 3, 37 -- t70ARdpEaIPLF_VByVuyfdBOkCnagU.6gsrNH2ULEKASSnHA5PgZ9yiAevA. 3, 37 -- Juv.9FacS4NQaa.iU.A5V.02e4HDL8tW_NOwVNQ0lOPIHPE1UUHaWz1zJEd5 3, 37 -- xkCPMobF0dY9slTVQFD9kWmjKzUWkx3kKoJhWRY0edrogE5QmqWMnyaTBCfz 3, 37 -- YAOYoD1qyfctPJQUjPoY4y8wMXLsl9ZIuuAnI2iKowy9e2QM12UECsz6Th2G 3, 37 -- wf04SD1T.16Y3NZWVJeKPretPE8WTaIX1GGqVvkaUZu.IEd9TZpTked9azPP 3, 37 -- UYE7CGG_5pz2CDFbdjqfT9MBI8PSMQxbDZVz_fiTnAOLWcMcJbzRf4uEoh44 3, 37 -- UzkP0CfN.FOagdpiCMHj.RxoiZ_fbQin5PKRYGwS_G4_GGoQeaIEU6fKikxw 3, 37 -- CKNq.FnCM90BMcIY7kJDOYcYF2NsNbdbSms0LJ60mXtYghrVk5cZUiA-- 3, 37 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Wed, 02 Nov 2011 07:51:45 PDT 3, 37 -- X-Mailer: YahooMailWebService/0.8.115.325013 3, 37 -- Message-ID: {1320245505.71843.YahooMailNeo-at-web110807.mail.gq1.yahoo.com} 3, 37 -- Date: Wed, 2 Nov 2011 07:51:45 -0700 (PDT) 3, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 37 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 37 -- Subject: ePetri dish: your opinion? 3, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 37 -- MIME-Version: 1.0 3, 37 -- Content-Type: text/plain; charset=iso-8859-1 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA2EpjtZ023808 ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 23, 26 -- From frank_karl-at-ardl.com Thu Nov 3 07:37:29 2011 23, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 23, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA3CbTE5031340 23, 26 -- for {microscopy-at-microscopy.com} ; Thu, 3 Nov 2011 07:37:29 -0500 23, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 23, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 3 Nov 23, 26 -- 2011 08:37:28 -0400 23, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 23, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 23, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 23, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 23, 26 -- Date: Thu, 3 Nov 2011 08:37:27 -0400 23, 26 -- Subject: RE: [Microscopy] ePetri dish: your opinion? 23, 26 -- Thread-Topic: [Microscopy] ePetri dish: your opinion? 23, 26 -- Thread-Index: AcyZcFF68hfDvA9IT5KwZ2OchHm/2AAr2otw 23, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010B0D13C2-at-exchange2k7.ad.ardl.com} 23, 26 -- In-Reply-To: {201111021501.pA2F1T5N007053-at-ns.microscopy.com} 23, 26 -- Accept-Language: en-US 23, 26 -- Content-Language: en-US 23, 26 -- X-MS-Has-Attach: 23, 26 -- X-MS-TNEF-Correlator: 23, 26 -- acceptlanguage: en-US 23, 26 -- Content-Type: text/plain; charset="iso-8859-1" 23, 26 -- MIME-Version: 1.0 23, 26 -- Content-Transfer-Encoding: 8bit 23, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA3CbTE5031340 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both E.young-at-warwick.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: E.young-at-warwick.ac.uk
Name: Emily Young
Organization: University of Warwick
Title-Subject: [Filtered] Schottky Emitter
Message: Hello,
I'm an undergraduate student at the University of Warwick, and I am attempting to accurately simulate a typical Schottky emitter used in modern SEMs. To do so, I really need to know at least a general idea of the geometry involved, so I was hoping you could help me.
So far, I have a good idea of the emitter tip diameter, the suppressor cap aperture diameter, and how far the tip protrudes from the supressor cap. What I still need to know is how thick the supressor cap typically is, how far away the anode is from the emitter tip, and how thick said anode is. All I need are general ballpark figures, for a very typical SEM arrangement. The hope is that I will be able to simulate the emitter successfully enough that a visual guide to the trajectories of the electrons through the microscope can be generated.
If you can offer any help, it would be greatly appreciated.
Hi everybody, We have a Jeol 100S electron microscope and now the level of RP oil is low. Anybody know if to refill is necessary that the microscope is turn on or turn off?. Thanks in advance. -- Dr. Armando Obregón Herrera Departamento de Biología, Edificio I P.B. División de Ciencias Naturales y Exactas Universidad de Guanajuato, Campus Gto. Tel. (473)7320006 Ext. 8127 Guanajuato, Gto. México.
==============================Original Headers============================== 2, 26 -- From yodapesister-at-gmail.com Thu Nov 3 14:32:56 2011 2, 26 -- Received: from mail-pz0-f41.google.com (mail-pz0-f41.google.com [209.85.210.41]) 2, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA3JWtnm006995 2, 26 -- for {microscopy-at-microscopy.com} ; Thu, 3 Nov 2011 14:32:55 -0500 2, 26 -- Received: by pzk36 with SMTP id 36so3997049pzk.0 2, 26 -- for {microscopy-at-microscopy.com} ; Thu, 03 Nov 2011 12:32:55 -0700 (PDT) 2, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 26 -- d=gmail.com; s=gamma; 2, 26 -- h=mime-version:date:message-id:subject:from:to:content-type 2, 26 -- :content-transfer-encoding; 2, 26 -- bh=aBZvMot9na/sA1UCHOR6eK2ZqIvxBXM02Rh01ELDPnI=; 2, 26 -- b=RkzrLl/7Ftig0E2GgDR+o5haEmEmGLXIsr67CrIH+PdH0ncgB2BE6DbTfjWjx00UnM 2, 26 -- rDG547jTlk9fUCpSNQRY01lpBqzovk9JCFeNpcQc9Grew67PHFSS/TxrRONdgrLLkL5L 2, 26 -- Ut3QuZ0w2Pws21E0alv+9oANaA7+go9wJQ2D0= 2, 26 -- MIME-Version: 1.0 2, 26 -- Received: by 10.182.114.35 with SMTP id jd3mr2187222obb.13.1320348774838; Thu, 2, 26 -- 03 Nov 2011 12:32:54 -0700 (PDT) 2, 26 -- Received: by 10.182.226.4 with HTTP; Thu, 3 Nov 2011 12:32:54 -0700 (PDT) 2, 26 -- Date: Thu, 3 Nov 2011 13:32:54 -0600 2, 26 -- Message-ID: {CA+mOmWHSC4QunEmWmNAHii2UGcLx=355J5jg-sqOAq-Mf8+xZA-at-mail.gmail.com} 2, 26 -- Subject: A question 2, 26 -- From: =?ISO-8859-1?Q?Armando_Obreg=F3n?= {yodapesister-at-gmail.com} 2, 26 -- To: microscopy-at-microscopy.com 2, 26 -- Content-Type: text/plain; charset=ISO-8859-1 2, 26 -- Content-Transfer-Encoding: 8bit 2, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA3JWtnm006995 ==============================End of - Headers==============================
We top off our RP oil pumps while they are running. This should be not problem. The filler plug is at the top of the unit and you can just carefully pour oil in.
However--when was the last time you changed the oil? We replace our RP oil yearly. Shut down TEM and replace oil, you may want to replace the belt at that time, then power all backup.
Good luck. Roseann
Roseann Csencsits, PhD Scientist in Charge, Donner TEM Facility Lawrence Berkeley Lab 01-365 1 Cyclotron Road Berkeley, CA 94720 510-486-4548
On Nov 3, 2011, at 12:41 PM, yodapesister-at-gmail.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi everybody, } We have a Jeol 100S electron microscope and now the level of RP oil is } low. Anybody know if to refill is necessary that the microscope is } turn on or turn off?. Thanks in advance. } -- } Dr. Armando Obregón Herrera } Departamento de Biología, Edificio I P.B. } División de Ciencias Naturales y Exactas } Universidad de Guanajuato, Campus Gto. } Tel. (473)7320006 Ext. 8127 } Guanajuato, Gto. México. }
==============================Original Headers============================== 18, 32 -- From rcsencsits-at-lbl.gov Thu Nov 3 14:48:39 2011 18, 32 -- Received: from ironport4.lbl.gov (ironport4.lbl.gov [128.3.41.45]) 18, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA3JmdcH023918 18, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 3 Nov 2011 14:48:39 -0500 18, 32 -- X-Ironport-SBRS: 5.2 18, 32 -- X-IronPort-Anti-Spam-Filtered: true 18, 32 -- X-IronPort-Anti-Spam-Result: Aj4CAKvvsk7RVdy0mGdsb2JhbABBA5pKjhiBGQgiAQEBAQEICQ0HFCWBcgEBAQQSAjorEAsMGCIhEAMBBQEcGR0EAYdolzAKnTaGCYI0YgSICJZLgn49hA8 18, 32 -- X-IronPort-AV: E=Sophos;i="4.69,451,1315206000"; 18, 32 -- d="scan'208";a="55865854" 18, 32 -- Received: from mail-vx0-f180.google.com ([209.85.220.180]) 18, 32 -- by ironport4.lbl.gov with ESMTP; 03 Nov 2011 12:48:38 -0700 18, 32 -- Received: by vcbfl10 with SMTP id fl10so531573vcb.39 18, 32 -- for {Microscopy-at-microscopy.com} ; Thu, 03 Nov 2011 12:48:38 -0700 (PDT) 18, 32 -- Received: by 10.220.16.204 with SMTP id p12mr825258vca.189.1320349717881; 18, 32 -- Thu, 03 Nov 2011 12:48:37 -0700 (PDT) 18, 32 -- Received: from apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov (apple-0-17-f2-2d-d1-b7.dhcp.lbl.gov. [131.243.35.54]) 18, 32 -- by mx.google.com with ESMTPS id c10sm10953816yhj.2.2011.11.03.12.48.36 18, 32 -- (version=TLSv1/SSLv3 cipher=OTHER); 18, 32 -- Thu, 03 Nov 2011 12:48:37 -0700 (PDT) 18, 32 -- References: {201111031941.pA3JfeTk020721-at-ns.microscopy.com} 18, 32 -- In-Reply-To: {201111031941.pA3JfeTk020721-at-ns.microscopy.com} 18, 32 -- Mime-Version: 1.0 (Apple Message framework v1084) 18, 32 -- Content-Type: text/plain; charset=iso-8859-1 18, 32 -- Message-Id: {247062AE-D88F-45AA-B237-CEEEF4EDFADD-at-lbl.gov} 18, 32 -- Cc: Microscopy-at-microscopy.com 18, 32 -- From: Roseann Csencsits {rcsencsits-at-lbl.gov} 18, 32 -- Subject: Re: [Microscopy] A question-RP oil 18, 32 -- Date: Thu, 3 Nov 2011 12:48:35 -0700 18, 32 -- To: yodapesister-at-gmail.com 18, 32 -- X-Mailer: Apple Mail (2.1084) 18, 32 -- Content-Transfer-Encoding: 8bit 18, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA3JmdcH023918 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both sm185210-at-ncr.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: sm185210-at-ncr.com
Name: Shannon McGhinnis
Organization: NCR
Title-Subject: [Filtered] 4Pi detector for Tescan SEM
Message: Does anyone know of a service company for SEMs and Xray detectors in the southeast (around Knoxville TN)?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Jjhill100-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: Jjhill100-at-gmail.com
Name: Jennifer J. Hill
Organization: JJ Hill Photography
Title-Subject: [Filtered] TEM-protocols for skeletal and cardiac muscle tissues in silicone
Message: Hello All,
I am a scientific photographer from Florida trying to help a colleague with some TEM specimen preparation of heart and skeletal muscle tissues. I am not a biologist by any means, but am hoping for some expertise from others in this field. If anyone can point me in the right direction for a methodology/protocol with recipes that has worked for him/her in the past, I would be most grateful.
I would like to add that my colleague has run an initial specimen preparation and he believes the amount of softening to hardness agent ratios may have been off--causing the silicone to be more soft than normal. He also is experiencing separation between the accelerator and silicone solution-is there a better way for him to add the accelerator? Directly, after the dehydration step or combine the silicone with the accelerator? Also, what is the maximum temperature in (C) in which these tissues can be cured?
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both smargiocchi-at-peoplexpert.fr as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Recruitment of a Pre-Sales Engineer in TEM
Message: Hi,
----------------------------------------------------------- PRE-SALES ENGINEER / TRANSMISSION ELECTRON MICROSCOPY CDI - Location: Yvelines (France) – Salary : according to profile and experience
PEOPLEXPERT (www.peoplexpert.fr) recruits for the French subsidiary (140 employees spread over Europe) of a company leader in the design of electronic devices of observations and measurements in high-tech (2000 employees ). Those devices are used in many industries, including research. You are a support to the sales team during the visit of clients or prospects. Knowing the value of our products (transmission electron microscopes), their characteristics relative to other tools and what is possible to do with, you explain the specificities to your audience and adapt your speech to their expectations and constraints. You also bring your expertise in our tendering answers. You will travel all over the Europe and work all the time in an international context (Poland, Czech Republic, Hungary, Italy, Greece ...).
With an education like Bachelor or Master degree, you have a solid understanding of the technical environment of transmission electron microscopy, either through work experience (sales, design or consulting) or through a thesis (or PhD) in which you had familiarized yourself with those tools extensively. You need to speak English.
Thank you for applying with the following email address: smargiocchi-at-peoplexpert.fr under: AVI / BSM -----------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both nesmicroscopy-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] SAVE-THE-DATE: NESM 45th Annual Fall Symposium -at- Gordon College, Dec. 1
Message: Greetings Microscopy Listserver-ites,
This is your friendly SAVE-THE-DATE reminder for NESM's 45th Annual Fall Symposium hosted by Gordon College on December 1, 2011. Stay tuned for detailed information about the Fall Symposium in our upcoming E-Newsletter. You can also keep up with NESM activities by checking us out of Facebook and Twitter (see links below).
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jrkjack-at-aol.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jrkjack-at-aol.com
Name: Judy King
Organization: Charleston Area Medical Center
Title-Subject: [Filtered] TEM - Need used TEM
Message: Does anyone know of a used TEM for sale? We need one for biological use - kidney biopsies.
Frank, I haven't seen any comments, but is it so different from pixel, line or frame averaging? I wasn't paying close attention to the details but what do you think about all the manipulation that goes on to give electron or x-ray tomography? I'm thinking that as long as what is being done is well documented and reasonably well understood, it shouldn't present any more problems than, for example, an SEM micrograph. I've always loved SEMs because if you tell me what you want to see, I can probably show it to you, but then we'd have to have a long talk about what is real, and that's without any digital manipulation.
I agree that we need to be on the lookout for artifacts and being led astray, but photography has served us well in spite of burning and dodging, and digital photography seems to be doing OK, too, although more skepticism is warranted.
Ken Converse owner
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 kenconverse-at-qualityimages.biz qualityimages.biz
-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: Thursday, November 03, 2011 8:42 AM To: kenconverse-at-qualityimages.biz
At the risk of exposing my own ignorance let me ask the group: Doesn't the use of computer processing of several low resolution images to make a "new" high resolution images trouble you? Would not/could not a artifact be manipulated (un-intentionally) into something that sends the researcher down blind alleys? I suppose this question has been asked shortly after Fox invented photography and we wondered if it would replace camera lucida drawings.
My TEM camera is the pits, so I "smooth" the image. I can see the pixal change at the edge of the particles, but I'm just measuring the particle diameter and the size categories are big enough. But I would be very uncomfortable looking at structure followed by evaluation fine structure or image information on a highly processed image.
So, am I out in left field with my concerns?
stay safe..............................
Frank
-----Original Message----- X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Sent: Wednesday, November 02, 2011 10:01 AM To: Frank Karl
Dear colleagues,
I enjoy very much the present time, when engineering, physics and mathemics have come to a maturity which allows all kinds of new microscopy techniques to be developed.
I came across an interesting article in PNAS: http://www.pnas.org/content/early/2011/09/26/1110681108.full.pdf
I think it is interesting for life and material scientists. As a biologist I don't understand all the technological intricacies of the method but I wonder if, given the simple material they used to get this kind of pictures, the resolution couldn't be further improved by using more sophisticated material (smaller CMOS pixel size for example?).
No personal interest, just an impressed reader ;-)
Stephane
==============================Original Headers============================== 3, 37 -- From nizets2-at-yahoo.com Wed Nov 2 09:51:46 2011 3, 37 -- Received: from nm5.bullet.mail.sp2.yahoo.com (nm5.bullet.mail.sp2.yahoo.com [98.139.91.75]) 3, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pA2EpjtZ023808 3, 37 -- for {microscopy-at-microscopy.com} ; Wed, 2 Nov 2011 09:51:46 -0500 3, 37 -- Received: from [98.139.91.70] by nm5.bullet.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- Received: from [98.139.91.54] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- Received: from [127.0.0.1] by omp1054.mail.sp2.yahoo.com with NNFMP; 02 Nov 2011 14:51:45 -0000 3, 37 -- X-Yahoo-Newman-Property: ymail-3 3, 37 -- X-Yahoo-Newman-Id: 687278.77456.bm-at-omp1054.mail.sp2.yahoo.com 3, 37 -- Received: (qmail 47852 invoked by uid 60001); 2 Nov 2011 14:51:45 -0000 3, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1320245505; bh=yabWDXlfrIwzg4acn5iDHEzVZwc1/owU33LgFNt6Vls=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIM E-Version:Content-Type:Content-Transfer-Encoding; b=EdAPzz2sy02QW6ivyj5oYV33faY4w1xteyUunrmJ7F/RYcEU3f/oR5NLO4uvyikt/Uik/sxlT8 RY/+mZMMPiCMl1BItEDu3MeWSPNdWBVPUr/Q0ew4eVDNJC1SSUccxrVnbmK3CE+lKkNZPFTNlE1E sltpcqqatwq1J/gZQHo78= 3, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 3, 37 -- s=s1024; d=yahoo.com; 3, 37 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIM E-Version:Content-Type:Content-Transfer-Encoding; 3, 37 -- b=Ii7uwhS07lb3HuzScExxxVMcTOq859gys91dExnVRenMtYSupDei5ihokXwfy1NUZr2kEF3r/t JeyI6vqhQ95F7mtQ/4TwFofp/rYrICK13esLiy23/EnTyaSfIi0ldDDJf5cyEgw7kzcFXT12cE0N qh2/PBimda0nxiDSnvC7Q=; 3, 37 -- X-YMail-OSG: ciwWW8sVM1m2_Q8wB9V3DePWv0jjFYi7DffZH_N4UaGs_8z 3, 37 -- yTzEbCzbijLvqGa4yGUjm21AhjapOopKDQybzEq5BsmVjUC_Dcm6rH66pG34 3, 37 -- t70ARdpEaIPLF_VByVuyfdBOkCnagU.6gsrNH2ULEKASSnHA5PgZ9yiAevA. 3, 37 -- Juv.9FacS4NQaa.iU.A5V.02e4HDL8tW_NOwVNQ0lOPIHPE1UUHaWz1zJEd5 3, 37 -- xkCPMobF0dY9slTVQFD9kWmjKzUWkx3kKoJhWRY0edrogE5QmqWMnyaTBCfz 3, 37 -- YAOYoD1qyfctPJQUjPoY4y8wMXLsl9ZIuuAnI2iKowy9e2QM12UECsz6Th2G 3, 37 -- wf04SD1T.16Y3NZWVJeKPretPE8WTaIX1GGqVvkaUZu.IEd9TZpTked9azPP 3, 37 -- UYE7CGG_5pz2CDFbdjqfT9MBI8PSMQxbDZVz_fiTnAOLWcMcJbzRf4uEoh44 3, 37 -- UzkP0CfN.FOagdpiCMHj.RxoiZ_fbQin5PKRYGwS_G4_GGoQeaIEU6fKikxw 3, 37 -- CKNq.FnCM90BMcIY7kJDOYcYF2NsNbdbSms0LJ60mXtYghrVk5cZUiA-- 3, 37 -- Received: from [80.122.101.100] by web110807.mail.gq1.yahoo.com via HTTP; Wed, 02 Nov 2011 07:51:45 PDT 3, 37 -- X-Mailer: YahooMailWebService/0.8.115.325013 3, 37 -- Message-ID: {1320245505.71843.YahooMailNeo-at-web110807.mail.gq1.yahoo.com} 3, 37 -- Date: Wed, 2 Nov 2011 07:51:45 -0700 (PDT) 3, 37 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 37 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 37 -- Subject: ePetri dish: your opinion? 3, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 3, 37 -- MIME-Version: 1.0 3, 37 -- Content-Type: text/plain; charset=iso-8859-1 3, 37 -- Content-Transfer-Encoding: 8bit 3, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA2EpjtZ023808 ==============================End of - Headers==============================
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 23, 26 -- From frank_karl-at-ardl.com Thu Nov 3 07:37:29 2011 23, 26 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 23, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA3CbTE5031340 23, 26 -- for {microscopy-at-microscopy.com} ; Thu, 3 Nov 2011 07:37:29 -0500 23, 26 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 23, 26 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 3 Nov 23, 26 -- 2011 08:37:28 -0400 23, 26 -- From: Frank Karl {frank_karl-at-ardl.com} 23, 26 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 23, 26 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 23, 26 -- Return-Receipt-To: {frank_karl-at-ardl.com} 23, 26 -- Date: Thu, 3 Nov 2011 08:37:27 -0400 23, 26 -- Subject: RE: [Microscopy] ePetri dish: your opinion? 23, 26 -- Thread-Topic: [Microscopy] ePetri dish: your opinion? 23, 26 -- Thread-Index: AcyZcFF68hfDvA9IT5KwZ2OchHm/2AAr2otw 23, 26 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010B0D13C2-at-exchange2k7.ad.ardl.com} 23, 26 -- In-Reply-To: {201111021501.pA2F1T5N007053-at-ns.microscopy.com} 23, 26 -- Accept-Language: en-US 23, 26 -- Content-Language: en-US 23, 26 -- X-MS-Has-Attach: 23, 26 -- X-MS-TNEF-Correlator: 23, 26 -- acceptlanguage: en-US 23, 26 -- Content-Type: text/plain; charset="iso-8859-1" 23, 26 -- MIME-Version: 1.0 23, 26 -- Content-Transfer-Encoding: 8bit 23, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA3CbTE5031340 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 28 -- From kenconverse-at-qualityimages.biz Sun Nov 6 12:21:44 2011 34, 28 -- Received: from cdptpa-omtalb.mail.rr.com (cdptpa-omtalb.mail.rr.com [75.180.132.121]) 34, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA6ILiF3004694 34, 28 -- for {microscopy-at-microscopy.com} ; Sun, 6 Nov 2011 12:21:44 -0600 34, 28 -- X-Authority-Analysis: v=2.0 cv=YYg/Fntf c=1 sm=0 a=nqrs8wXCc9jj42pMp0m8Gw==:17 a=vPu_sHE3rEEA:10 a=kj9zAlcOel0A:10 a=YdczCmdkAAAA:8 a=Zx37jsudAAAA:8 a=CjxXgO3LAAAA:8 a=LML2UYdAAAAA:8 a=cnzhEAA3RcWv8YbX8mMA:9 a=FkZERUcr59CZM-qsx5cA:7 a=CjuIK1q_8ugA:10 a=5JUlSvKOtR0A:10 a=_HDdLMu04s0A:10 a=C3J4oLNe8gwA:10 a=Bd5jmW48H-gA:10 a=RyjLG6jaqdoA:10 a=3_2eAsHTqJoA:10 a=C6KWKcr4_fMA:10 a=rC2wZJ5BpNYA:10 a=G91thX30KR8A:10 a=oi4whU4Oel_j--xf:21 a=L63Xa3v-MERBACmU:21 a=nqrs8wXCc9jj42pMp0m8Gw==:117 34, 28 -- X-Cloudmark-Score: 0 34, 28 -- X-Originating-IP: 72.224.149.57 34, 28 -- Received: from [72.224.149.57] ([72.224.149.57:2363] helo=Ken) 34, 28 -- by cdptpa-oedge03.mail.rr.com (envelope-from {kenconverse-at-qualityimages.biz} ) 34, 28 -- (ecelerity 2.2.3.46 r()) with ESMTP 34, 28 -- id A3/51-17438-730D6BE4; Sun, 06 Nov 2011 18:21:44 +0000 34, 28 -- From: "Ken Converse" {kenconverse-at-qualityimages.biz} 34, 28 -- To: {frank_karl-at-ardl.com} , "MSA Listserver" {microscopy-at-microscopy.com} 34, 28 -- Subject: RE: [Microscopy] RE: ePetri dish: your opinion? 34, 28 -- Date: Sun, 6 Nov 2011 13:21:33 -0500 34, 28 -- Message-ID: {F5AE8FDB902B4F759CDCA1EEFB49EF3F-at-Ken} 34, 28 -- MIME-Version: 1.0 34, 28 -- Content-Type: text/plain; 34, 28 -- charset="us-ascii" 34, 28 -- X-Priority: 3 (Normal) 34, 28 -- X-MSMail-Priority: Normal 34, 28 -- X-Mailer: Microsoft Outlook, Build 10.0.6863 34, 28 -- Importance: Normal 34, 28 -- Thread-Index: AcyaJfRMcafqksjfQ06GUzBY3UEjbACiU1kg 34, 28 -- In-Reply-To: {201111031241.pA3CfdX5002400-at-ns.microscopy.com} 34, 28 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6109 34, 28 -- Content-Transfer-Encoding: 8bit 34, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pA6ILiF3004694 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both henrik.kaker-at-guest.arnes.si as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: henrik.kaker-at-guest.arnes.si Name: Henrik Kaker
We have a file from one of our customer in the INCA project file format. We need a help to exporting this data into TIFF files and Oxford spectrum files.
==============================Original Headers============================== 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 7 17:01:08 2011 11, 25 -- Received: from znl.com ([206.69.208.20]) 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA7N18Pl018316 11, 25 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2011 17:01:08 -0600 11, 25 -- Received: from localhost (localhost [127.0.0.1]) 11, 25 -- by znl.com (Postfix) with ESMTP id 4BAE543ABCF 11, 25 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2011 17:01:08 -0600 (CST) 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 11, 25 -- Received: from znl.com ([127.0.0.1]) 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 11, 25 -- with ESMTP id 4Y3gExhYStU1 for {microscopy-at-microscopy.com} ; 11, 25 -- Mon, 7 Nov 2011 17:01:07 -0600 (CST) 11, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 11, 25 -- by znl.com (Postfix) with ESMTPA id C844443ABC4 11, 25 -- for {microscopy-at-microscopy.com} ; Mon, 7 Nov 2011 17:01:07 -0600 (CST) 11, 25 -- Message-ID: {4EB86333.1090201-at-microscopy.com} 11, 25 -- Date: Mon, 07 Nov 2011 17:01:07 -0600 11, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 11, 25 -- Reply-To: henrik.kaker-at-guest.arnes.si 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 11, 25 -- MIME-Version: 1.0 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 11, 25 -- Subject: viaWWW:Exporting INCA project file 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From vdgsnl-at-aol.nl Mon Nov 7 21:37:18 2011 Return-Path: {vdgsnl-at-aol.nl} Received: from drews5thbestdomain.com (da.14.364a.static.theplanet.com [74.54.20.218]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA83bHGc006437; Mon, 7 Nov 2011 21:37:18 -0600 Received: from drews5thbestdomain.com (localhost.localdomain [127.0.0.1]) by drews5thbestdomain.com (Postfix) with ESMTP id D88ED10C31D2C; Mon, 7 Nov 2011 11:20:23 -0600 (CST) Received: from User (dhcp-077-249-026-011.chello.nl [77.249.26.11]) by drews5thbestdomain.com (Postfix) with ESMTP; Mon, 7 Nov 2011 11:20:23 -0600 (CST) Reply-To: {tksuntangk-at-aol.com}
Hi all
Neighbor collegues here in Strasbourg have an Siemens Elmiscope 101, dating from 1972 which is looking for a new home. It has been working until 1999-2000, as far I know. The film chamber (under the screen chamber) is missing. It seems to me that it could be more usefull as a source of spares or as an art piece (could be very nice in the entrance hall of a moderne buiding ;-) ) than as a working instrument...
Of coarse it is free for who comes to get it. It must go away until the end of the year. If no one is interested, it will be scraped:'( . If someone needs a few precise pieces, I can put them on side, when we'll dismantle it.
Hoping for a new home for it !
Jacques
-- Veuillez prendre note de la nouvelle adresse mail.
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both amy.stobart-at-jeoluk.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Message: Job Vacancy Applications Specialist - Electron Optics
Job Type: Full time Location: Welwyn Garden City, UK
JEOL are a leading supplier of Scanning Electron Microscopes (SEMs), Transmission Electron Microscopes (TEMs), Scanning Probe Microscopes (SPMs), Mass Spectrometers, NMR spectrometers, and Semiconductor tools for scientific and industrial purposes. We provide applications-specific solutions that advance our customers' diverse objectives — from routine analysis of organic and inorganic specimens to breakthroughs in nanotechnological development.
We are looking to recruit an Applications Specialist for Electron Microscope and related instruments. The key responsibilities will be, 1) Demonstrating the instruments for sales promotion
2) The analysis of samples which are received for the purposes of instrument evaluation
3) Supporting workshop events, exhibitions and training courses
The ideal applicant will have previous experience of SEM and TEM in an academic or industrial setting (although complete training will be provided on all instrumentation). Good presentation skills and the ability to communicate to people with a wide range of technical abilities are essential. A bachelorÂ’s degree in a scientific discipline is a minimum requirement. This position is based at our demonstration facility in Welwyn Garden City in the UK, however regular travel within the UK and abroad will be required. To apply, please send your C.V. with a cover letter to Amy Stobart (Applications by Email are welcome. amy.stobart-at-jeoluk.com)
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pA8EEVhO007257 for {MicroscopyListserverArchive2-at-microscopy.com} ; Tue, 8 Nov 2011 08:14:31 -0600 Received: (from mail-at-localhost) by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id pA8EEVK6007253; Tue, 8 Nov 2011 08:14:31 -0600
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both yashasin-at-rocketmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: yashasin-at-rocketmail.com Name: Kazem
Organization: IMPRC
Title-Subject: [Filtered] Au analyzing
Message: Hi all Analyzing the Magnetite and pyrite with EPMA SX100 shows 1 to 1.5% Au in some points, is it natural or may be wrong? also our condition is: 15Kv, 20 nA and beam size 3um. and in BSE pictures have not been observed any visible gold. How much is the high grade Au concentration in Magnetite and Pyrite? there are some results: Point Fe Co Ni Au Total 1 / 1 . 61.27 0.23 1.05 1.29 66.03 2 / 1 . 60.95 0.42 0.05 0.93 63.57 3 / 1 . 53.95 0 0 1.43 56.4
Sheldon H. Moll, age 77, of Bedford, MA, passed away peacefully at Emerson Hospital on November 5, 2011 while surrounded by his family after a lengthy illness.
After earning his Ph.D. in Materials Science and Physics from MIT in 1959, he continued living in Massachusetts where he helped found, and was a Sr. VP for Amray, Inc. of Bedford, MA. After 40 years, Dr. Moll retired after the sale of the company. In his position he traveled the world where the company and he were well known throughout the Scanning Electron Microscopy field. Dr. Moll wrote over 120 publications and technical letters.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tbogea-at-mail.ubc.ca Name: Tami Bogea
Organization: University of British Columbia
Title-Subject: [Filtered] Carbonate-free NaOH
Message: Thanks to you all for your comments and suggestions regarding my ImmunoEM questions. One brief one: apparently only EMS supplies a carbonate-free solution of sodium hydroxide. Does anyone know how to obtain carbonate-free sodium hydroxide(in pellets)?
-- Mary Raven Microscopy Facility Director Neuroscience Research Institute& Molecular, Cellular& Developmental Biology Biology 2 Building, room 5173 The University of California Santa Barbara, CA 93106-5060
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both martham-at-uci.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: martham-at-uci.edu Name: Martha Mecartney
Organization: UC Irvine
Title-Subject: [Filtered] Need Cryo-TEM GATAN pump station for 1990 CM20 holder
Message: Anyone have an older version of the GATAN cryo-TEM pumping station for a CM20 cryo-transfer holder from 1990? We are revitalizing our cryo-TEM with vitrified samples. The newer models from GATAN do not have the right o-ring size or connectors, so if you have an old version for trade or sale or know where we could find one, please let me know.
Martha Mecartney Professor of Chemical Engineering and Materials Science University of California martham-at-uci.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tbogea-at-mail.ubc.ca Name: Tami Bogea
Organization: University of British Columbia
Title-Subject: [Filtered] carbonate-free NaOH
Message: Thanks so much for your comments and suggestions regarding the carbonate-free NaOH. I'll run a few tests next week and hopefully the problem will be solved.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
We use a Gatan double tilt holder, there have been repeatedly a couple of accidents over the years which a user managed to bend the front tip at where the specimen cradle is.
The front tip part appears to be aluminium, the sample cradle connects to the holder via two tiny brass pins. Allowing the space for cradle and notch clearance for X-ray escape, the main holder part is apparently very weak. The accident sees the rod tip being bended exactly at the weak points where the two pins connect the cradle and serve as pivot points.
Unfortunately, none of my users owned up to it. We struggle to figure out the causes of the accidents, so we donnot know what to improve to avoid it. We certainly do not want to repair again and again for the same problem.
We would appreciate any ideas or advice on how this could happen, if we can repair this by ourselves, and how to prevent it in the future.
Being a user facility with more than 50 TEM users, I can sympathize with the problem of broken double-tilt holders. We have had this occur several times in the past.
One solution has been to fabricate a clamping mechanism that holds the 2-t holder securely on the bench-top stand during the loading of sample. It is most important to prevent any rocking/rotating of the holder during loading as this produces stresses on the pivot pins, causing breakage.
The second important solution is to provide good support under the sample cup during sample loading. Gatan and newer FEI stands typically have the holder tip extending out into free space. We reverted to using the older FEI/Philips stands that provide support for the holder tip. Since introducing the these changes, we have had no breakage during the loading step.
(I can offer no help for breakage due to heavy-handed or slippery fingered users, other than suggesting my method of applying the "user alignment tool"- a large wooden mallet.)
Cheers, Roger
Roger A. Ristau, PhD Electron Microscopy Specialist Institute of Materials Science 97 North Eagleville Road University of Connecticut Storrs, CT 06269 vox: 860-486-5453 fax: 860-486-4745
} From: {microscopylistserver-noreply-at-microscopy.com} } Reply-To: {microscopylistserver-noreply-at-microscopy.com} } Date: Fri, 11 Nov 2011 07:50:47 -0600 } To: {raristau-at-ims.uconn.edu} } Subject: [Microscopy] viaWWW:Broken Gatan Double Tilt Holder } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both } z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: z.zhou-at-lboro.ac.uk Name: Z Zhou } } Organization: Loughborough University, UK } } Title-Subject: [Filtered] Gatan Double Tilt Holder } } Message: Dear Fellow TEM Microscopists, } } We use a Gatan double tilt holder, there have been repeatedly a couple of } accidents over the years } which a user managed to bend the front tip at where the specimen cradle is. } } The front tip part appears to be aluminium, the sample cradle connects to the } holder via two tiny } brass pins. Allowing the space for cradle and notch clearance for X-ray } escape, the main holder part } is apparently very weak. The accident sees the rod tip being bended exactly at } the weak points where } the two pins connect the cradle and serve as pivot points. } } Unfortunately, none of my users owned up to it. We struggle to figure out the } causes of the } accidents, so we donnot know what to improve to avoid it. We certainly do not } want to repair again } and again for the same problem. } } We would appreciate any ideas or advice on how this could happen, if we can } repair this by } ourselves, and how to prevent it in the future. } } Zhou } } Login Host: 131.231.108.233 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Nov 11 07:45:38 } 2011 } 13, 25 -- Received: from znl.com ([206.69.208.20]) } 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } pABDjcBF021781 } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:38 -0600 } 13, 25 -- Received: from localhost (localhost [127.0.0.1]) } 13, 25 -- by znl.com (Postfix) with ESMTP id BFEF943DA5A } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:38 -0600 } (CST) } 13, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 25 -- Received: from znl.com ([127.0.0.1]) } 13, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } 10024) } 13, 25 -- with ESMTP id LOPgSsSzpUGU for {microscopy-at-microscopy.com} ; } 13, 25 -- Fri, 11 Nov 2011 07:45:37 -0600 (CST) } 13, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 25 -- by znl.com (Postfix) with ESMTPA id B041C43DA4F } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:37 -0600 } (CST) } 13, 25 -- Message-ID: {4EBD2701.4080303-at-microscopy.com} } 13, 25 -- Date: Fri, 11 Nov 2011 07:45:37 -0600 } 13, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 13, 25 -- Reply-To: z.zhou-at-lboro.ac.uk } 13, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 25 -- MIME-Version: 1.0 } 13, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 25 -- Subject: viaWWW:Broken Gatan Double Tilt Holder } 13, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 10, 23 -- From raristau-at-ims.uconn.edu Fri Nov 11 15:50:40 2011 10, 23 -- Received: from mta1.uits.uconn.edu (mta1.uits.uconn.edu [137.99.25.234]) 10, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pABLodfc008111 10, 23 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 15:50:40 -0600 10, 23 -- Received: from [137.99.19.185] (d19h185.public.uconn.edu [137.99.19.185]) 10, 23 -- by mta1.uits.uconn.edu (Postfix) with ESMTP id C02B034C84; 10, 23 -- Fri, 11 Nov 2011 09:41:54 -0500 (EST) 10, 23 -- User-Agent: Microsoft-Entourage/11.4.0.080122 10, 23 -- Date: Fri, 11 Nov 2011 09:41:52 -0500 10, 23 -- Subject: Re: [Microscopy] viaWWW:Broken Gatan Double Tilt Holder 10, 23 -- From: Roger Ristau {raristau-at-ims.uconn.edu} 10, 23 -- To: "Microscopy-at-Microscopy.Com" {microscopy-at-microscopy.com} , 10, 23 -- {z.zhou-at-lboro.ac.uk} 10, 23 -- Message-ID: {CAE29E60.33D6%raristau-at-ims.uconn.edu} 10, 23 -- Thread-Topic: [Microscopy] viaWWW:Broken Gatan Double Tilt Holder 10, 23 -- Thread-Index: AcyggAzsS8d4uwxzEeG9yAAbY55CVA== 10, 23 -- In-Reply-To: {201111111350.pABDolxu027376-at-ns.microscopy.com} 10, 23 -- Mime-version: 1.0 10, 23 -- Content-type: text/plain; 10, 23 -- charset="US-ASCII" 10, 23 -- Content-transfer-encoding: 7bit 10, 23 -- X-Virus-Scanned: clamav-milter 0.96.5 at mta1 10, 23 -- X-Virus-Status: Clean ==============================End of - Headers==============================
Sigh. The anonymous cack-handed klutz strikes again.
Someone recently mounted something large on our VP-SEM stage and rammed it up into the BSE detector, producing a hairline crack across 2 quadrants. It took us a while to figure out what was wrong, ramming something into the detector was outside our thoughtspace. And of course, no-one owned up, though we are pretty sure when it happened = who the culprit was because we could see when image quality got really bad. Hard to believe that a couple of users accepted this poor image quality without comment.
One way to try to track this down is to compel all users to comment on the status of the instrument when they start their session. This is quite good for figuring out who gets oil all over objectives, for example. For serious damage, you end up paying for it no matter what, and we have compelled all of our SEM users to be retrained, and set the stage stop way down...
You could insist that all of your double tilt holder users be retrained, and have a policy that they report on its status at the beginning of each session and see if that helps. Even if you don't track down the culprit(s), you might reduce the number of incidents.
good luck,
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 12/11/11 12:52 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 13, 32 -- From prvs=290bfcd0b=Rosemary.White-at-csiro.au Sat Nov 12 03:32:14 2011 13, 32 -- Received: from vic-MTAout1.csiro.au (vic-MTAout1.csiro.au [150.229.64.37]) 13, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAC9WC8U000916 13, 32 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 03:32:13 -0600 13, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 13, 32 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 13, 32 -- s=email; t=1321090333; x=1352626333; 13, 32 -- h=date:subject:from:to:cc:message-id:in-reply-to: 13, 32 -- mime-version:content-transfer-encoding; 13, 32 -- bh=iuOPh962ko3CtKu1vFBIYOkiKqdn2GsJuxaJILORqY4=; 13, 32 -- b=plj9g02YDTKoOGafyJYunQ8OiSHntJ5vJ0YcRK0XOUqvwM7BFAhfGXz3 13, 32 -- WWOXzLsA91rD5FAuvEaNZN+Lwo7tRxdTybTrTJz/o0mTmuqATXNiYd5Hi 13, 32 -- YPtNaXmWIo6SFLV; 13, 32 -- X-IronPort-AV: E=Sophos;i="4.69,498,1315144800"; 13, 32 -- d="scan'208";a="72281983" 13, 32 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 13, 32 -- by vic-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 12 Nov 2011 20:32:10 +1100 13, 32 -- Received: from [152.83.195.117] (152.83.195.117) by 13, 32 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 13, 32 -- (TLS) id 8.3.192.1; Sat, 12 Nov 2011 20:32:10 +1100 13, 32 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 13, 32 -- Date: Sat, 12 Nov 2011 20:32:07 +1100 13, 32 -- Subject: damage in general - was Broken Gatan Double Tilt Holder 13, 32 -- From: Rosemary White {rosemary.white-at-csiro.au} 13, 32 -- To: {microscopy-at-microscopy.com} 13, 32 -- CC: {z.zhou-at-lboro.ac.uk} 13, 32 -- Message-ID: {CAE48473.4B77F%rosemary.white-at-csiro.au} 13, 32 -- Thread-Topic: damage in general - was Broken Gatan Double Tilt Holder 13, 32 -- In-Reply-To: {201111111352.pABDqobh029514-at-ns.microscopy.com} 13, 32 -- MIME-Version: 1.0 13, 32 -- Content-Type: text/plain; charset="US-ASCII" 13, 32 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We experience EXACTLY the same thing. The only way of preventing it is to have a competent user load the samples and insert the holder. Fixing it yourself is very difficult, the tip typically needs replacing when it is bent like this. I am wondering if it is not possible to fabricate the tip from titanium to increase its durability. I suspect that that would increase the replacement cost considerably. ___
John Mansfield PhD Cphys MInstP Microanalysis Society - President Associate Director North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
On Nov 11, 2011, at 8:56 AM, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: z.zhou-at-lboro.ac.uk Name: Z Zhou } } Organization: Loughborough University, UK } } Title-Subject: [Filtered] Gatan Double Tilt Holder } } Message: Dear Fellow TEM Microscopists, } } We use a Gatan double tilt holder, there have been repeatedly a couple of accidents over the years } which a user managed to bend the front tip at where the specimen cradle is. } } The front tip part appears to be aluminium, the sample cradle connects to the holder via two tiny } brass pins. Allowing the space for cradle and notch clearance for X-ray escape, the main holder part } is apparently very weak. The accident sees the rod tip being bended exactly at the weak points where } the two pins connect the cradle and serve as pivot points. } } Unfortunately, none of my users owned up to it. We struggle to figure out the causes of the } accidents, so we donnot know what to improve to avoid it. We certainly do not want to repair again } and again for the same problem. } } We would appreciate any ideas or advice on how this could happen, if we can repair this by } ourselves, and how to prevent it in the future. } } Zhou } } Login Host: 131.231.108.233 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Nov 11 07:45:38 2011 } 13, 25 -- Received: from znl.com ([206.69.208.20]) } 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pABDjcBF021781 } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:38 -0600 } 13, 25 -- Received: from localhost (localhost [127.0.0.1]) } 13, 25 -- by znl.com (Postfix) with ESMTP id BFEF943DA5A } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:38 -0600 (CST) } 13, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 25 -- Received: from znl.com ([127.0.0.1]) } 13, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 13, 25 -- with ESMTP id LOPgSsSzpUGU for {microscopy-at-microscopy.com} ; } 13, 25 -- Fri, 11 Nov 2011 07:45:37 -0600 (CST) } 13, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 25 -- by znl.com (Postfix) with ESMTPA id B041C43DA4F } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:37 -0600 (CST) } 13, 25 -- Message-ID: {4EBD2701.4080303-at-microscopy.com} } 13, 25 -- Date: Fri, 11 Nov 2011 07:45:37 -0600 } 13, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 13, 25 -- Reply-To: z.zhou-at-lboro.ac.uk } 13, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 25 -- MIME-Version: 1.0 } 13, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 25 -- Subject: [Filtered] viaWWW:Broken Gatan Double Tilt Holder } 13, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } }
==============================Original Headers============================== 10, 27 -- From microscopylistserver-noreply-at-microscopy.com Sat Nov 12 06:50:47 2011 10, 27 -- Received: from znl.com ([206.69.208.20]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pACColSK020401 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 06:50:47 -0600 10, 27 -- Received: from localhost (localhost [127.0.0.1]) 10, 27 -- by znl.com (Postfix) with ESMTP id 8593843E5E7 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 06:50:47 -0600 (CST) 10, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 27 -- Received: from znl.com ([127.0.0.1]) 10, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 27 -- with ESMTP id P9L1NBRUsW95 for {microscopy-at-microscopy.com} ; 10, 27 -- Sat, 12 Nov 2011 06:50:38 -0600 (CST) 10, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 27 -- by znl.com (Postfix) with ESMTPA id 3E34643E5D7 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 06:50:38 -0600 (CST) 10, 27 -- Message-ID: {4EBE6B9D.5040707-at-microscopy.com} 10, 27 -- Date: Sat, 12 Nov 2011 06:50:37 -0600 10, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 27 -- Reply-To: John Mansfield {jfmjfm-at-umich.edu} 10, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 27 -- MIME-Version: 1.0 10, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 27 -- Subject: [Filtered] Re: [Microscopy] viaWWW:Broken Gatan Double Tilt Holder 10, 27 -- References: {201111111356.pABDu94p002748-at-ns.microscopy.com} 10, 27 -- In-Reply-To: {201111111356.pABDu94p002748-at-ns.microscopy.com} 10, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
From swiss_nedloto-at-inmail24.com Sat Nov 12 20:40:07 2011 Return-Path: {swiss_nedloto-at-inmail24.com} Received: from hmarco.servername.com (htextiles.org [192.217.122.134] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAD2e7Xc016506; Sat, 12 Nov 2011 20:40:07 -0600 Message-Id: {201111130240.pAD2e7Xc016506-at-ns.microscopy.com} Received: from 46-129-65-98.dynamic.upc.nl ([46.129.65.98] helo=User) by hmarco.servername.com with esmtpa (Exim 4.69) (envelope-from {swiss_nedloto-at-inmail24.com} ) id 1RPPxh-0004Cl-L1; Sun, 13 Nov 2011 05:38:26 +0300 Reply-To: {vaarnwmn-at-aol.com}
Dear listers,
I just want to ask if anyone of you had their BX41/51 microscopes in the lab fitted with Canon EOS cameras? If yes, where did you get your adaptors? I have inquired with the Olympus local supplier but he told me that no such adaptor exist at their end. Of course, they would only cater to Olympus cameras but it's worth a try asking than leave me with "what if's".
Anyway, I did some google search and found these (see links below). However, before we spend $$$ on buying these items, I just want to inquire if this will actually work and which Canon EOS camera specifically would this fit into.
Melina Miralles Lab Technician The Petroleum Institute
==============================Original Headers============================== 9, 42 -- From mmiralles-at-pi.ac.ae Sun Nov 13 01:25:01 2011 9, 42 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 9, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAD7P0G4018936 9, 42 -- for {microscopy-at-microscopy.com} ; Sun, 13 Nov 2011 01:25:01 -0600 9, 42 -- X-IronPort-AV: E=Sophos;i="4.69,502,1315166400"; 9, 42 -- d="scan'208";a="6462925" 9, 42 -- Received: from unknown (HELO pi-exf2.PI.AC.AE) ([192.168.2.13]) 9, 42 -- by mx1.pi.ac.ae with ESMTP; 13 Nov 2011 10:29:16 +0400 9, 42 -- Received: from pi-exm.PI.AC.AE ([192.168.2.10]) by pi-exf2.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 9, 42 -- Sun, 13 Nov 2011 11:30:42 +0400 9, 42 -- Received: from SRV03002.PI.AC.AE ([10.248.1.58]) by pi-exm.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.4675); 9, 42 -- Sun, 13 Nov 2011 11:30:41 +0400 9, 42 -- Received: from SRV03003.PI.AC.AE ([fe80::d436:79b9:ecc9:aa76]) by 9, 42 -- SRV03002.PI.AC.AE ([::1]) with mapi id 14.01.0339.001; Sun, 13 Nov 2011 9, 42 -- 11:30:41 +0400 9, 42 -- From: Melina Miralles {mmiralles-at-pi.ac.ae} 9, 42 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 9, 42 -- Subject: Canon EOS Camera in BX41/51 microscopes? 9, 42 -- Thread-Topic: Canon EOS Camera in BX41/51 microscopes? 9, 42 -- Thread-Index: Acyh1iCiLHOHk6MyQgGHqYjAh7oetA== 9, 42 -- Date: Sun, 13 Nov 2011 07:30:40 +0000 9, 42 -- Message-ID: {EDD97900BF67864787C12BC4DFFA4B3C01179C-at-SRV03003.PI.AC.AE} 9, 42 -- Accept-Language: en-US 9, 42 -- Content-Language: en-US 9, 42 -- X-MS-Has-Attach: 9, 42 -- X-MS-TNEF-Correlator: 9, 42 -- x-cr-hashedpuzzle: ASSE C9TZ DUXk DjOI EG1s FAq9 Fg7j FxWK GQDI GTMK GroY 9, 42 -- ID8c JRai JtSl Lkf+ 9, 42 -- Mqm2;1;bQBpAGMAcgBvAHMAYwBvAHAAeQBAAG0AaQBjAHIAbwBzAGMAbwBwAHkALgBjAG8AbQA=;Sosha1_v1;7;{3ABC1776-6D88-4B74-9C30-2D15057669EB};bQBtAGkAcgBhAGwAbABlAHMAQABwAGkALgBhAGMALgBhAGUA;Sun, 9, 42 -- 13 Nov 2011 07:30:32 9, 42 -- GMT;QwBhAG4AbwBuACAARQBPAFMAIABDAGEAbQBlAHIAYQAgAGkAbgAgAEIAWAA0ADEALwA1ADEAIABtAGkAYwByAG8AcwBjAG8AcABlAHMAPwA= 9, 42 -- x-cr-puzzleid: {3ABC1776-6D88-4B74-9C30-2D15057669EB} 9, 42 -- x-originating-ip: [10.248.21.53] 9, 42 -- x-tm-as-product-ver: SMEX-10.0.0.4211-6.800.1017-18514.005 9, 42 -- x-tm-as-result: No--38.504200-8.000000-31 9, 42 -- x-tm-as-user-approved-sender: No 9, 42 -- x-tm-as-user-blocked-sender: No 9, 42 -- Content-Type: text/plain; charset="us-ascii" 9, 42 -- MIME-Version: 1.0 9, 42 -- X-OriginalArrivalTime: 13 Nov 2011 07:30:41.0768 (UTC) FILETIME=[25E41680:01CCA1D6] 9, 42 -- Content-Transfer-Encoding: 8bit 9, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAD7P0G4018936 ==============================End of - Headers==============================
From advhnsthissen-at-gmail.com Sun Nov 13 05:43:24 2011 Return-Path: {advhnsthissen-at-gmail.com} Received: from hmarco.servername.com (htextiles.org [192.217.122.134] (may be forged)) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pADBhOsa022397; Sun, 13 Nov 2011 05:43:24 -0600 Message-Id: {201111131143.pADBhOsa022397-at-ns.microscopy.com} Received: from 46-129-65-98.dynamic.upc.nl ([46.129.65.98] helo=User) by hmarco.servername.com with esmtpa (Exim 4.69) (envelope-from {advhnsthissen-at-gmail.com} ) id 1RPYSH-0000Qi-JN; Sun, 13 Nov 2011 14:42:34 +0300 Reply-To: {advhnsthissen-at-aol.com}
Olympus says it has no adapters? Sounds like you asked the wrong question or the sales rep was on drugs. They have mounts for the Nikon F-mount. I know I have several. They used to have the Canon C-mount (the F-mounts will work in a pinch). What they do not have and do not exist are the mounts which make the scope "compu" compatible. Basically the scope become a fully manual lens. It will not auto-focus and it will not adjust the aperture (this should be a "Duh") Set to Aperture priority or manual and they work fine.
Richard E. Edelmann, Ph.D. Center for Advanced Microscopy & Imaging, Director Miami University Oxford, OH 45056
Ph: 513.529.5712 www.cami.muohio.edu ________________________________________ X-from: mmiralles-at-pi.ac.ae [mmiralles-at-pi.ac.ae] Sent: Sunday, November 13, 2011 2:25 AM To: Edelmann, Richard E. Dr.
Dear listers,
I just want to ask if anyone of you had their BX41/51 microscopes in the lab fitted with Canon EOS cameras? If yes, where did you get your adaptors? I have inquired with the Olympus local supplier but he told me that no such adaptor exist at their end. Of course, they would only cater to Olympus cameras but it's worth a try asking than leave me with "what if's".
Anyway, I did some google search and found these (see links below). However, before we spend $$$ on buying these items, I just want to inquire if this will actually work and which Canon EOS camera specifically would this fit into.
Apologies, the Canon lens mount is the is the Canon EF or EF-S mount not C-mount (the video camera standard).
Richard E. Edelmann, Ph.D. Center for Advanced Microscopy & Imaging, Director Miami University Oxford, OH 45056
Ph: 513.529.5712 www.cami.muohio.edu ________________________________________ X-from: edelmare-at-muohio.edu [edelmare-at-muohio.edu] Sent: Sunday, November 13, 2011 1:05 PM To: Edelmann, Richard E. Dr.
Olympus says it has no adapters? Sounds like you asked the wrong question or the sales rep was on drugs. They have mounts for the Nikon F-mount. I know I have several. They used to have the Canon C-mount (the F-mounts will work in a pinch). What they do not have and do not exist are the mounts which make the scope "compu" compatible. Basically the scope become a fully manual lens. It will not auto-focus and it will not adjust the aperture (this should be a "Duh") Set to Aperture priority or manual and they work fine.
Richard E. Edelmann, Ph.D. Center for Advanced Microscopy & Imaging, Director Miami University Oxford, OH 45056
Ph: 513.529.5712 www.cami.muohio.edu ________________________________________ X-from: mmiralles-at-pi.ac.ae [mmiralles-at-pi.ac.ae] Sent: Sunday, November 13, 2011 2:25 AM To: Edelmann, Richard E. Dr.
Dear listers,
I just want to ask if anyone of you had their BX41/51 microscopes in the lab fitted with Canon EOS cameras? If yes, where did you get your adaptors? I have inquired with the Olympus local supplier but he told me that no such adaptor exist at their end. Of course, they would only cater to Olympus cameras but it's worth a try asking than leave me with "what if's".
Anyway, I did some google search and found these (see links below). However, before we spend $$$ on buying these items, I just want to inquire if this will actually work and which Canon EOS camera specifically would this fit into.
I use Canon cameras on all of my microscopes. For the AX70, which is similar to the BX series, no optics are needed, just a tube that mounts one to the other. The full visible image circle is recorded on the camera, and then some. The flange-to-flange distance is roughly 60mm. I started with an empty Olympus tube of unknown purpose and mounted an EF lens flange on the camera side. And since the tube was too long anyway, I had a machinist cut it mid-length and thread one to the other to make the parfocal adjustment. A few radial cuts in the female section allows a hose clamp to lock it in the adjusted position like a collet closer on a lathe. For camera control, I use DSLR Remote Pro from Breeze Systems which displays the live view from the camera. It all works like a charm.
} Dear listers, } } I just want to ask if anyone of you had their BX41/51 microscopes in the lab fitted with Canon EOS cameras? } If yes, where did you get your adaptors? I have inquired with the Olympus local supplier but he told me that no such adaptor exist at their end. Of course, they would only cater to Olympus cameras but it's worth a try asking than leave me with "what if's". } } Anyway, I did some google search and found these (see links below). However, before we spend $$$ on buying these items, I just want to inquire if this will actually work and which Canon EOS camera specifically would this fit into. } } (http://webstore.diaginc.com/DD12BXT-1-2X-Coupler-p/wsn-dd12bxt.htm) } (http://webstore.diaginc.com/EOSC-T2-p/ws-eosc-t2.htm) } } I would be very grateful for any information. } } Thank you and best regards, } } Melina Miralles } Lab Technician } The Petroleum Institute
-- Bernard R. Cuzzillo, Ph.D., P.E. President, Mechanical Engineer, and Fire Scientist Berkeley Research Company (BRC) 600 Addison Street Berkeley, CA 94710-1920 USA
Gatan do offer a titanium tip replacement for their double tilt holders. I had to have this done to repair one of our specimen rods that had achieved a rather impressive 25 degree bend at the pivot points. The main contributing factors were using a specimen rod cradle that did not clamp the rod and excessive muscle reflex when the rod started to tilt. I now only use the Gatan clamping stand when changing specimens in their specimen rod.
When I had the repair done about 10 years ago, the cost was ~$3500, which was about $1000 more than the standard tip.
Philip L. Flaitz IBM Microelectronics, Hopewell Junction, NY Ph.......(845) 892-3094, FAX -6256 pager - 800-608-9398 flaitz-at-us.ibm.com
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
We experience EXACTLY the same thing. The only way of preventing it is to have a competent user load the samples and insert the holder. Fixing it yourself is very difficult, the tip typically needs replacing when it is bent like this. I am wondering if it is not possible to fabricate the tip from titanium to increase its durability. I suspect that that would increase the replacement cost considerably. ___
John Mansfield PhD Cphys MInstP Microanalysis Society - President Associate Director North Campus Electron Microbeam Analysis Laboratory 417 SRB, University of Michigan 2455 Hayward, Ann Arbor MI 48109-2143 USA Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 (Leaving a phone message at 936-3352 is preferable to 834-3913) Email: jfmjfm-at-engin.umich.edu URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° 42.6439') AIM: thejfmjfm Yahoo: thejfmjfm Skype: thejfmjfm
Home address: 4304 Spring Lake Boulevard Ann Arbor MI 48108-9657 Phone (734) 994-3096 Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' 48" (-83 ° 45.7980')
Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.
On Nov 11, 2011, at 8:56 AM, microscopylistserver-noreply-at-microscopy.com wrote:
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------------
} } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: z.zhou-at-lboro.ac.uk Name: Z Zhou } } Organization: Loughborough University, UK } } Title-Subject: [Filtered] Gatan Double Tilt Holder } } Message: Dear Fellow TEM Microscopists, } } We use a Gatan double tilt holder, there have been repeatedly a couple of accidents over the years } which a user managed to bend the front tip at where the specimen cradle is. } } The front tip part appears to be aluminium, the sample cradle connects to the holder via two tiny } brass pins. Allowing the space for cradle and notch clearance for X-ray escape, the main holder part } is apparently very weak. The accident sees the rod tip being bended exactly at the weak points where } the two pins connect the cradle and serve as pivot points. } } Unfortunately, none of my users owned up to it. We struggle to figure out the causes of the } accidents, so we donnot know what to improve to avoid it. We certainly do not want to repair again } and again for the same problem. } } We would appreciate any ideas or advice on how this could happen, if we can repair this by } ourselves, and how to prevent it in the future. } } Zhou } } Login Host: 131.231.108.233 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Nov 11 07:45:38 2011 } 13, 25 -- Received: from znl.com ([206.69.208.20]) } 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pABDjcBF021781 } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:38 -0600 } 13, 25 -- Received: from localhost (localhost [127.0.0.1]) } 13, 25 -- by znl.com (Postfix) with ESMTP id BFEF943DA5A } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:38 -0600 (CST) } 13, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 13, 25 -- Received: from znl.com ([127.0.0.1]) } 13, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 13, 25 -- with ESMTP id LOPgSsSzpUGU for {microscopy-at-microscopy.com} ; } 13, 25 -- Fri, 11 Nov 2011 07:45:37 -0600 (CST) } 13, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 13, 25 -- by znl.com (Postfix) with ESMTPA id B041C43DA4F } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 07:45:37 -0600 (CST) } 13, 25 -- Message-ID: {4EBD2701.4080303-at-microscopy.com} } 13, 25 -- Date: Fri, 11 Nov 2011 07:45:37 -0600 } 13, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 13, 25 -- Reply-To: z.zhou-at-lboro.ac.uk } 13, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 13, 25 -- MIME-Version: 1.0 } 13, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 25 -- Subject: [Filtered] viaWWW:Broken Gatan Double Tilt Holder } 13, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 13, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } }
==============================Original Headers============================== 10, 27 -- From microscopylistserver-noreply-at-microscopy.com Sat Nov 12 06:50:47 2011 10, 27 -- Received: from znl.com ([206.69.208.20]) 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pACColSK020401 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 06:50:47 -0600 10, 27 -- Received: from localhost (localhost [127.0.0.1]) 10, 27 -- by znl.com (Postfix) with ESMTP id 8593843E5E7 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 06:50:47 -0600 (CST) 10, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 27 -- Received: from znl.com ([127.0.0.1]) 10, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 27 -- with ESMTP id P9L1NBRUsW95 for {microscopy-at-microscopy.com} ; 10, 27 -- Sat, 12 Nov 2011 06:50:38 -0600 (CST) 10, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 27 -- by znl.com (Postfix) with ESMTPA id 3E34643E5D7 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 06:50:38 -0600 (CST) 10, 27 -- Message-ID: {4EBE6B9D.5040707-at-microscopy.com} 10, 27 -- Date: Sat, 12 Nov 2011 06:50:37 -0600 10, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 27 -- Reply-To: John Mansfield {jfmjfm-at-umich.edu} 10, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 27 -- MIME-Version: 1.0 10, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 27 -- Subject: [Filtered] Re: [Microscopy] viaWWW:Broken Gatan Double Tilt Holder 10, 27 -- References: {201111111356.pABDu94p002748-at-ns.microscopy.com} 10, 27 -- In-Reply-To: {201111111356.pABDu94p002748-at-ns.microscopy.com} 10, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
==============================Original Headers============================== 37, 27 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 14 08:16:24 2011 37, 27 -- Received: from znl.com ([206.69.208.20]) 37, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAEEGO5n019140 37, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:16:24 -0600 37, 27 -- Received: from localhost (localhost [127.0.0.1]) 37, 27 -- by znl.com (Postfix) with ESMTP id 19EA643FF21 37, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:16:24 -0600 (CST) 37, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 37, 27 -- Received: from znl.com ([127.0.0.1]) 37, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 37, 27 -- with ESMTP id zEAq5WcwAebC for {microscopy-at-microscopy.com} ; 37, 27 -- Mon, 14 Nov 2011 08:16:21 -0600 (CST) 37, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 37, 27 -- by znl.com (Postfix) with ESMTPA id 92E0E43FF11 37, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:16:21 -0600 (CST) 37, 27 -- Message-ID: {4EC122B5.8090500-at-microscopy.com} 37, 27 -- Date: Mon, 14 Nov 2011 08:16:21 -0600 37, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 37, 27 -- Reply-To: Philip Flaitz {flaitz-at-us.ibm.com} 37, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 37, 27 -- MIME-Version: 1.0 37, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 37, 27 -- Subject: ] Re: viaWWW:Broken Gatan Double Tilt Holder 37, 27 -- References: {201111121252.pACCqLw5022942-at-ns.microscopy.com} 37, 27 -- In-Reply-To: {201111121252.pACCqLw5022942-at-ns.microscopy.com} 37, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 37, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both i.j.portman-at-warwick.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: i.j.portman-at-warwick.ac.uk Name: Ian Portman
Organization: University of Warwick
Title-Subject: [Filtered] Fixing water samples for posting
Message: Hi We're getting involved in some international projects with schools. Our basic plan is to get schools to send us samples of pond water from their local area so we can image the microbial life ( particularly bacteriophage ) present. Our main hurdle is posting water samples that may well contain some thoroughly unpleasant organisms between countries. The obvious answer is to fix the samples before posting but most fixatives aren't the sort of thing you can stick in the post without a lot of paperwork, nor are they the kind of thing school children should be handling. Is there a fixative suitable so that we may post out a kit to a school, they can just add the pond water and post it back without any headaches over hazardous goods shipping? We'd need about 5-10ml of water. I'm wondering if something such as isopropanol might be ok - sent out by us at 40% and sent back with pond water at 20% final conc. Cheers Ian
This is something for the community - please respond to Friedlander at tfriedlander-at-fmpharma.com . Not to me or by using "reply". Phil
} Date: Fri, 11 Nov 2011 14:36:07 -0800 } Reply-To: Thomas Friedlander {tfriedlander-at-fmpharma.com} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } Below is the result of your form, submitted on Friday, November 11, } 2011 at 02:36:05 PM. } } realname - Thomas Friedlander } Email - tfriedlander-at-fmpharma.com } ORGANIZATION - FMP } EDUCATION - Graduate College } LOCATION - Danbury, CT, USA } SUBJECT_OF_QUESTION - Micro manipulators } QUESTION - Hi, } } I have been working on developing an automated microscope platform } (joy stick controlled) with two 5 axis micro-manipulators for } manipulating samples for something called DAC loading (diamond anvil } cell). This technique is used to load samples in a device which can } be put under great pressure to study the molecular lattice within } the sample. Typically the samples are between 5 to 100 microns. They } are typically crystalline (rubies are about 5 microns for this type } of experiment). } } My question is "What other applications could an automated } microscope platform with dual joy stick controlled 5 axis } manipulators be good for?" } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Mon Nov 14 08:56:34 2011 2, 24 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAEEuXrl020864 2, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:56:34 -0600 2, 24 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 2, 24 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pAEEbLaN008369 2, 24 -- for {Microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 09:37:22 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Mon, 14 Nov 2, 24 -- 2011 09:56:32 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240802cae6d951c560-at-[141.209.160.249]} 2, 24 -- Date: Mon, 14 Nov 2011 09:56:30 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: Fwd: Ask-A-Microscopist Microscopy 5 axis manipulators 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 03FV2BmX1 - 94b501a5f5b4 - 20111114 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
Hello Everyone, I want to thank everyone for their advice and e-mails about repair of my glass knife maker. I have the options and now it's up to management. Thanks!!! Frank
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 6, 25 -- From frank_karl-at-ardl.com Mon Nov 14 09:34:53 2011 6, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 6, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAEFYr68006100 6, 25 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 09:34:53 -0600 6, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 6, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Mon, 14 6, 25 -- Nov 2011 10:34:52 -0500 6, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 6, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 6, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 6, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 6, 25 -- Date: Mon, 14 Nov 2011 10:34:51 -0500 6, 25 -- Subject: thanks for advice 6, 25 -- Thread-Topic: thanks for advice 6, 25 -- Thread-Index: Acyi4vNyfIRpAtrxRL+4wG2XHNMH6w== 6, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF15077010D973D89-at-exchange2k7.ad.ardl.com} 6, 25 -- Accept-Language: en-US 6, 25 -- Content-Language: en-US 6, 25 -- X-MS-Has-Attach: 6, 25 -- X-MS-TNEF-Correlator: 6, 25 -- acceptlanguage: en-US 6, 25 -- Content-Type: text/plain; charset="iso-8859-1" 6, 25 -- MIME-Version: 1.0 6, 25 -- Content-Transfer-Encoding: 8bit 6, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAEFYr68006100 ==============================End of - Headers==============================
From johnnarzika-at-hotmail.com Mon Nov 14 11:35:16 2011 Return-Path: {johnnarzika-at-hotmail.com} Received: from mail.yrbmagazine.com ([128.121.32.69]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAEHZF13027317; Mon, 14 Nov 2011 11:35:16 -0600 Message-Id: {201111141735.pAEHZF13027317-at-ns.microscopy.com} Received: from User ([82.128.62.252]) (authenticated user jim-at-yrbmagazine.com) by mail.yrbmagazine.com (Kerio MailServer 6.5.1); Mon, 14 Nov 2011 06:36:15 -0500 Reply-To: {obicollins-at-yahoo.cn}
Regarding "how to prevent this in the future", another option that I've seen work is to require all users of your double-tilt holder to "requalify" on its use. Basically that would mean the next time they sign up for instrument time and plan on using the holder, they need to be observed by you. While some users will be on their best behavior and mask holder-breaking practices, many times this exercise serves to demonstrate what users are doing right, and also what they're doing wrong, and can sometimes expose the practices which cause the breakage to begin with. This is not the same as retraining everyone, since retraining is interactive and can mask the problems, which simple observation/"requalification" has the opportunity to expose more issues. Just a thought that might help.
***************** John M. Papalia, Ph.D. EM Career Seeker jpapalia-at-papalia.net *****************
On 11/14/2011 9:34 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Gatan do offer a titanium tip replacement for their double tilt holders. I } had to have this done to repair one of our specimen rods that had achieved } a rather impressive 25 degree bend at the pivot points. The main } contributing factors were using a specimen rod cradle that did not clamp } the rod and excessive muscle reflex when the rod started to tilt. I now } only use the Gatan clamping stand when changing specimens in their specimen } rod. } } When I had the repair done about 10 years ago, the cost was ~$3500, which } was about $1000 more than the standard tip. } } } Philip L. Flaitz } IBM Microelectronics, Hopewell Junction, NY } Ph.......(845) 892-3094, FAX -6256 } pager - 800-608-9398 } flaitz-at-us.ibm.com } } } } } From: microscopylistserver-noreply-at-microscopy.com } } To: } Philip Flaitz/Fishkill/IBM-at-IBMUS } } Date: 11/13/2011 11:32 AM } } } Subject: [Filtered] [Microscopy] [Filtered] Re: viaWWW:Broken Gatan Double } Tilt Holder } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } We experience EXACTLY the same thing. The only way of preventing it is to } have a competent user } load the samples and insert the holder. } Fixing it yourself is very difficult, the tip typically needs replacing } when it is bent like this. I } am wondering if it is not possible to fabricate the tip from titanium to } increase its durability. I } suspect that that would increase the replacement cost considerably. } ___ } } John Mansfield PhD Cphys MInstP } Microanalysis Society - President } Associate Director } North Campus Electron Microbeam Analysis Laboratory } 417 SRB, University of Michigan } 2455 Hayward, Ann Arbor MI 48109-2143 USA } Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913 } (Leaving a phone message at 936-3352 is preferable to 834-3913) } Email: jfmjfm-at-engin.umich.edu } URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html } Location: N 42 ° 17' 38.3" (42 ° 17.6391' ) W 83 ° 42' 38.6" (-83 ° } 42.6439') } AIM: thejfmjfm } Yahoo: thejfmjfm } Skype: thejfmjfm } } Home address: } 4304 Spring Lake Boulevard } Ann Arbor MI 48108-9657 } Phone (734) 994-3096 } Location: N 42 ° 13' 28.8" (42 ° 13.4807') W 83 ° 45' 47.9" 42° 16' } 48" (-83 ° 45.7980') } } Please note: Electronic Mail is not secure, but should be read several } times every day, and should } definitely be used for urgent or sensitive issues. } } On Nov 11, 2011, at 8:56 AM, microscopylistserver-noreply-at-microscopy.com } wrote: } } } } } } } } } } } ---------------------------------------------------------------------------- } } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } } America } } } To Subscribe/Unsubscribe -- } } } http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } } } --------------------------------------------------------------------------- } } } Remember this posting is most likely not from a Subscriber, so when } } } replying please copy both } } } z.zhou-at-lboro.ac.uk as well as the MIcroscopy Listserver } } } } } --------------------------------------------------------------------------- } } } Email: z.zhou-at-lboro.ac.uk Name: Z Zhou } } } } Organization: Loughborough University, UK } } } } Title-Subject: [Filtered] Gatan Double Tilt Holder } } } } Message: Dear Fellow TEM Microscopists, } } } } We use a Gatan double tilt holder, there have been repeatedly a couple of } } } accidents over the years } } } which a user managed to bend the front tip at where the specimen cradle } } } is. } } } The front tip part appears to be aluminium, the sample cradle connects to } } } the holder via two tiny } } } brass pins. Allowing the space for cradle and notch clearance for X-ray } } } escape, the main holder part } } } is apparently very weak. The accident sees the rod tip being bended } } } exactly at the weak points where } } } the two pins connect the cradle and serve as pivot points. } } } } Unfortunately, none of my users owned up to it. We struggle to figure out } } } the causes of the } } } accidents, so we donnot know what to improve to avoid it. We certainly do } } } not want to repair again } } } and again for the same problem. } } } } We would appreciate any ideas or advice on how this could happen, if we } } } can repair this by } } } ourselves, and how to prevent it in the future. } } } } Zhou } } } } Login Host: 131.231.108.233 } } } } } --------------------------------------------------------------------------- } } } } } } } ==============================Original } } } Headers============================== } } } 13, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Nov 11 } } } 07:45:38 2011 } } } 13, 25 -- Received: from znl.com ([206.69.208.20]) } } 13, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) } } } with ESMTP id pABDjcBF021781 } } } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 } } } 07:45:38 -0600 } } } 13, 25 -- Received: from localhost (localhost [127.0.0.1]) } } 13, 25 -- by znl.com (Postfix) with ESMTP id BFEF943DA5A } } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 } } } 07:45:38 -0600 (CST) } } } 13, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 13, 25 -- Received: from znl.com ([127.0.0.1]) } } 13, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } } } (amavisd-new, port 10024) } } } 13, 25 -- with ESMTP id LOPgSsSzpUGU for } } } {microscopy-at-microscopy.com} ; } } } 13, 25 -- Fri, 11 Nov 2011 07:45:37 -0600 (CST) } } 13, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } } } [206.69.208.22]) } } } 13, 25 -- by znl.com (Postfix) with ESMTPA id B041C43DA4F } } 13, 25 -- for {microscopy-at-microscopy.com} ; Fri, 11 Nov 2011 } } } 07:45:37 -0600 (CST) } } } 13, 25 -- Message-ID: {4EBD2701.4080303-at-microscopy.com} } } 13, 25 -- Date: Fri, 11 Nov 2011 07:45:37 -0600 } } 13, 25 -- From: MicroscopyListserver-NoReply } } } {microscopylistserver-noreply-at-microscopy.com} } } } 13, 25 -- Reply-To: z.zhou-at-lboro.ac.uk } } 13, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } } } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } } 13, 25 -- MIME-Version: 1.0 } } 13, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 13, 25 -- Subject: [Filtered] viaWWW:Broken Gatan Double Tilt Holder } } 13, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 13, 25 -- Content-Transfer-Encoding: 7bit } } ==============================End of - } } } Headers============================== } } } } } } } } } } ==============================Original } Headers============================== } 10, 27 -- From microscopylistserver-noreply-at-microscopy.com Sat Nov 12 } 06:50:47 2011 } 10, 27 -- Received: from znl.com ([206.69.208.20]) } 10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with } ESMTP id pACColSK020401 } 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 } 06:50:47 -0600 } 10, 27 -- Received: from localhost (localhost [127.0.0.1]) } 10, 27 -- by znl.com (Postfix) with ESMTP id 8593843E5E7 } 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 } 06:50:47 -0600 (CST) } 10, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 27 -- Received: from znl.com ([127.0.0.1]) } 10, 27 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 10, 27 -- with ESMTP id P9L1NBRUsW95 for } {microscopy-at-microscopy.com} ; } 10, 27 -- Sat, 12 Nov 2011 06:50:38 -0600 (CST) } 10, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 10, 27 -- by znl.com (Postfix) with ESMTPA id 3E34643E5D7 } 10, 27 -- for {microscopy-at-microscopy.com} ; Sat, 12 Nov 2011 } 06:50:38 -0600 (CST) } 10, 27 -- Message-ID: {4EBE6B9D.5040707-at-microscopy.com} } 10, 27 -- Date: Sat, 12 Nov 2011 06:50:37 -0600 } 10, 27 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 10, 27 -- Reply-To: John Mansfield {jfmjfm-at-umich.edu} } 10, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 27 -- MIME-Version: 1.0 } 10, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 27 -- Subject: [Filtered] Re: [Microscopy] viaWWW:Broken Gatan Double } Tilt Holder } 10, 27 -- References: {201111111356.pABDu94p002748-at-ns.microscopy.com} } 10, 27 -- In-Reply-To: {201111111356.pABDu94p002748-at-ns.microscopy.com} } 10, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 27 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers============================== } } } } } } } } } ==============================Original Headers============================== } 37, 27 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 14 08:16:24 2011 } 37, 27 -- Received: from znl.com ([206.69.208.20]) } 37, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAEEGO5n019140 } 37, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:16:24 -0600 } 37, 27 -- Received: from localhost (localhost [127.0.0.1]) } 37, 27 -- by znl.com (Postfix) with ESMTP id 19EA643FF21 } 37, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:16:24 -0600 (CST) } 37, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 37, 27 -- Received: from znl.com ([127.0.0.1]) } 37, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 37, 27 -- with ESMTP id zEAq5WcwAebC for {microscopy-at-microscopy.com} ; } 37, 27 -- Mon, 14 Nov 2011 08:16:21 -0600 (CST) } 37, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 37, 27 -- by znl.com (Postfix) with ESMTPA id 92E0E43FF11 } 37, 27 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 08:16:21 -0600 (CST) } 37, 27 -- Message-ID: {4EC122B5.8090500-at-microscopy.com} } 37, 27 -- Date: Mon, 14 Nov 2011 08:16:21 -0600 } 37, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 37, 27 -- Reply-To: Philip Flaitz {flaitz-at-us.ibm.com} } 37, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 37, 27 -- MIME-Version: 1.0 } 37, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 37, 27 -- Subject: [Filtered] ] Re: viaWWW:Broken Gatan Double Tilt Holder } 37, 27 -- References: {201111121252.pACCqLw5022942-at-ns.microscopy.com} } 37, 27 -- In-Reply-To: {201111121252.pACCqLw5022942-at-ns.microscopy.com} } 37, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 37, 27 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 7, 28 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 14 20:05:12 2011 7, 28 -- Received: from znl.com ([206.69.208.20]) 7, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAF25Cs0003382 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 20:05:12 -0600 7, 28 -- Received: from localhost (localhost [127.0.0.1]) 7, 28 -- by znl.com (Postfix) with ESMTP id 3FAB144052B 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 20:05:12 -0600 (CST) 7, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 28 -- Received: from znl.com ([127.0.0.1]) 7, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 28 -- with ESMTP id 48szql7wKjHA for {microscopy-at-microscopy.com} ; 7, 28 -- Mon, 14 Nov 2011 20:05:10 -0600 (CST) 7, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 7, 28 -- by znl.com (Postfix) with ESMTPA id 469F6440520 7, 28 -- for {microscopy-at-microscopy.com} ; Mon, 14 Nov 2011 20:05:10 -0600 (CST) 7, 28 -- Message-ID: {4EC1C8D6.40909-at-microscopy.com} 7, 28 -- Date: Mon, 14 Nov 2011 20:05:10 -0600 7, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 28 -- Reply-To: John Papalia {jpapalia-at-papalia.net} 7, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 28 -- MIME-Version: 1.0 7, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 28 -- Subject: [Filtered] Re: [Microscopy] ] Re: viaWWW:Broken Gatan Double Tilt 7, 28 -- Holder 7, 28 -- References: {201111141434.pAEEYZFE019128-at-ns.microscopy.com} 7, 28 -- In-Reply-To: {201111141434.pAEEYZFE019128-at-ns.microscopy.com} 7, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 28 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
Thank you for all the replies that you have sent me. I will look into these adaptor providers and check. I understand about the installation of cameras in the trinocular mount (which we also need), however I would like to confirm if anyone of you has a setup wherein the camera is attached via the eyepieces.
Thanks again!
Dr. Edelman,
That is the answer that I got from Olympus guy. He said they have adaptors that would only fit Olympus cameras. He suggested me to ask Canon if they (Canon or any other brand of SLR camera) could provide adaptors that will fit into Olympus microscopes instead. But on their side, they are reluctant to entertain our idea of having another camera brand on the scopes. And I sincerely hope, he was not on drugs!
Melina Miralles Lab Technician The Petroleum Institute
==============================Original Headers============================== 11, 38 -- From mmiralles-at-pi.ac.ae Tue Nov 15 03:21:27 2011 11, 38 -- Received: from mx1.pi.ac.ae (mx1.pi.ac.ae [213.42.148.228]) 11, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAF9LQST025831 11, 38 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2011 03:21:27 -0600 11, 38 -- X-IronPort-AV: E=Sophos;i="4.69,513,1315166400"; 11, 38 -- d="scan'208";a="6484119" 11, 38 -- Received: from unknown (HELO pi-exf2.PI.AC.AE) ([192.168.2.13]) 11, 38 -- by mx1.pi.ac.ae with ESMTP; 15 Nov 2011 12:25:37 +0400 11, 38 -- Received: from pi-exm.PI.AC.AE ([192.168.2.10]) by pi-exf2.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.3959); 11, 38 -- Tue, 15 Nov 2011 13:27:21 +0400 11, 38 -- Received: from SRV03001.PI.AC.AE ([10.248.1.54]) by pi-exm.PI.AC.AE with Microsoft SMTPSVC(6.0.3790.4675); 11, 38 -- Tue, 15 Nov 2011 13:27:20 +0400 11, 38 -- Received: from SRV03003.PI.AC.AE ([fe80::d436:79b9:ecc9:aa76]) by 11, 38 -- SRV03001.PI.AC.AE ([::1]) with mapi id 14.01.0339.001; Tue, 15 Nov 2011 11, 38 -- 13:27:20 +0400 11, 38 -- From: Melina Miralles {mmiralles-at-pi.ac.ae} 11, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 11, 38 -- Subject: RE: [Microscopy] RE: Canon EOS Camera in BX41/51 microscopes? 11, 38 -- Thread-Topic: [Microscopy] RE: Canon EOS Camera in BX41/51 microscopes? 11, 38 -- Thread-Index: AQHMojAz9G2ETHcyKUuKxj1cFjpaBJWtqDLg 11, 38 -- Date: Tue, 15 Nov 2011 09:27:19 +0000 11, 38 -- Message-ID: {EDD97900BF67864787C12BC4DFFA4B3C012BC6-at-SRV03003.PI.AC.AE} 11, 38 -- References: {201111131809.pADI9R4r019701-at-ns.microscopy.com} 11, 38 -- In-Reply-To: {201111131809.pADI9R4r019701-at-ns.microscopy.com} 11, 38 -- Accept-Language: en-US 11, 38 -- Content-Language: en-US 11, 38 -- X-MS-Has-Attach: 11, 38 -- X-MS-TNEF-Correlator: 11, 38 -- x-originating-ip: [10.248.21.53] 11, 38 -- x-tm-as-product-ver: SMEX-10.0.0.4211-6.800.1017-18520.005 11, 38 -- x-tm-as-result: No--39.079000-8.000000-31 11, 38 -- x-tm-as-user-approved-sender: No 11, 38 -- x-tm-as-user-blocked-sender: No 11, 38 -- Content-Type: text/plain; charset="us-ascii" 11, 38 -- MIME-Version: 1.0 11, 38 -- X-OriginalArrivalTime: 15 Nov 2011 09:27:20.0324 (UTC) FILETIME=[C62E7C40:01CCA378] 11, 38 -- Content-Transfer-Encoding: 8bit 11, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAF9LQST025831 ==============================End of - Headers==============================
Recently we started to prepare cross-section SEM samples. We are using an old Buehler Ecomet III grinder.
I'm now looking for a modern precision lapping/polishing machine for SEM.
X-from a former workplace I know the Allied MultiPrep, which I think was wonderful.
Would such a machine be an overshoot for normal SEM preparation?
What other options are there on the market?
Manufacturers are invited to respond off-list.
I'm aware that a very similar question was asked last year. But then it was for TEM (see below).
Thanks in advance,
Bettina
-----------------------
Dear Listers,
A colleague of mine is trying to use immuno EM with NANOGOLD particles, but at the magnification that she needs, the 5 (or 10 nm) gold particles cannot be seen clearly. Could someone suggest a protocol (homemade better) for gold particles enhancement? (A protocol that uses silver, nickel or anything else). There are some kits already available, but she would prefer using her own reagents.
Thanks to all,
Best,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
though commerciable enhancers (e.g. from AURION) work very good, the best (= most sensitive) enhancement still can be achieved (at least in my hands) by the good, old home-made Danscher silver-intensification ( Light microscopic visualization of colloidal gold on resin-embedded tissue. Danscher G, Nörgaard JO.; J Histochem Cytochem. 1983 Dec;31(12):1394-8 )
in my hands 0.8 - 1.0 nm Au is enhanced in a 2-step intensification (6 minutes and 8 minutes); Quantum Dots ( 4-5 minutes and 5 - 7 minutes) all at 37° Celsius
for larger particles 7 plus 11 minutes could be applicable, but unspecific background will rise too
good luck, Peter Heimann ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
jmircheski-at-us.es: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear Listers, } } A colleague of mine is trying to use immuno EM with NANOGOLD particles, but } at the magnification that she needs, the 5 (or 10 nm) gold particles cannot } be seen clearly. Could someone suggest a protocol (homemade better) for gold } particles enhancement? (A protocol that uses silver, nickel or anything } else). There are some kits already available, but she would prefer using her } own reagents. } } Thanks to all, } } Best, } } Josif } } Dr. Josif Mircheski } ____________________________________________________________________________ } ___ } Instituto de Biomedicina de Sevilla (IBIS), Lab 108 } Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla } Avda. Manuel Siurot S/Nº } 41013 Sevilla } Spain } } Phone:+34-955923045 } e-mail: jmircheski-at-us.es } web: www.ibis-sevilla.es } } } } } ==============================Original Headers============================== } 9, 38 -- From jmircheski-at-us.es Tue Nov 15 05:51:10 2011 } 9, 38 -- Received: from mail.us.es (mail.us.es [193.147.175.20]) } 9, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAFBp9Uc000426 } 9, 38 -- for {microscopy-at-microscopy.com} ; Tue, 15 Nov 2011 05:51:09 -0600 } 9, 38 -- Received: (qmail 26049 invoked from network); 15 Nov 2011 12:51:08 +0100 } 9, 38 -- Received: from unknown (HELO us.es) (192.168.2.12) } 9, 38 -- by us.es with SMTP; 15 Nov 2011 12:51:08 +0100 } 9, 38 -- Received: (qmail 10388 invoked by uid 507); 15 Nov 2011 11:51:02 -0000 } 9, 38 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on antivirus2 } 9, 38 -- X-Spam-Level: * } 9, 38 -- X-Spam-Status: No, score=1.6 required=7.5 tests=BAYES_50,DOS_OUTLOOK_TO_MX, } 9, 38 -- FSL_HELO_NON_FQDN_1,HELO_NO_DOMAIN autolearn=disabled version=3.3.1 } 9, 38 -- Received: from 127.0.0.1 by antivirus2 (envelope-from {jmircheski-at-us.es} , uid 501) with qmail-scanner-2.08 } 9, 38 -- (clamdscan: 0.97.3/13949. } 9, 38 -- Clear:RC:1(127.0.0.1):. } 9, 38 -- Processed in 0.034851 secs); 15 Nov 2011 11:51:02 -0000 } 9, 38 -- Received: from unknown (HELO antivirus2) (127.0.0.1) } 9, 38 -- by us.es with SMTP; 15 Nov 2011 11:51:02 -0000 } 9, 38 -- Received: from 192.168.1.13 (192.168.1.13) } 9, 38 -- by antivirus2 (F-Secure/fsigk_smtp/406/antivirus2); } 9, 38 -- Tue, 15 Nov 2011 12:51:02 +0100 (CET) } 9, 38 -- X-Virus-Status: clean(F-Secure/fsigk_smtp/406/antivirus2) } 9, 38 -- Received: (qmail 4604 invoked from network); 15 Nov 2011 12:51:02 +0100 } 9, 38 -- Received: from chacon4.us.es (HELO cubiculos) (jmircheski-at-150.214.11.24) } 9, 38 -- by us.es with RC4-MD5 encrypted SMTP; 15 Nov 2011 12:51:02 +0100 } 9, 38 -- From: "Josif Mircheski" {jmircheski-at-us.es} } 9, 38 -- To: {microscopy-at-microscopy.com} } 9, 38 -- Subject: Nanoparticles enhancement for immuno EM } 9, 38 -- Date: Tue, 15 Nov 2011 12:51:08 +0100 } 9, 38 -- Message-ID: {008401cca38c$dde0d4f0$99a27ed0$-at-es} } 9, 38 -- MIME-Version: 1.0 } 9, 38 -- Content-Type: text/plain; } 9, 38 -- charset="iso-8859-1" } 9, 38 -- X-Mailer: Microsoft Office Outlook 12.0 } 9, 38 -- Thread-Index: AcyjjNu3T/3Ftfu5SKmy7VUIGe9HGA== } 9, 38 -- Content-Language: en-us } 9, 38 -- Content-Transfer-Encoding: 8bit } 9, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAFBp9Uc000426 } ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eoptics-at-mcmaster.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eoptics-at-mcmaster.ca Name: Fred Pearson
Organization: McMaster University
Title-Subject: [Filtered] TEM Gatan DT Stage Damage Message: Hi I have the same DT Gatan Stage since 1990........we have had repairs to it many times over the years. } From your description and my experience with this your email suggests that the user pulled the holder or bent it sideways from the goniometer before it cleared the insertion hole. I tell the students to withdraw the stage totally away from the microscope goniometer stage before moving away from the microscope.
I am a big fan of do it yourself in regards to things like this but would go with the kit approach in this situation. I am a big fan of the Nanoprobes gold (not silver) enhancement kit. It is much easier and more reproducible than the silver enhancement kits or "homebrew" approaches I used in the dark ages. Good luck. Tom
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: jmircheski-at-us.es [mailto:jmircheski-at-us.es] Sent: Tuesday, November 15, 2011 5:52 AM To: Phillips, Thomas E.
Dear Listers,
A colleague of mine is trying to use immuno EM with NANOGOLD particles, but at the magnification that she needs, the 5 (or 10 nm) gold particles cannot be seen clearly. Could someone suggest a protocol (homemade better) for gold particles enhancement? (A protocol that uses silver, nickel or anything else). There are some kits already available, but she would prefer using her own reagents.
Thanks to all,
Best,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
Having polished welds in steel and aluminum, solder joints not to mention stainless steel and other metals for SEM and LM, I recommend Struers. They may be a little more expensive, but they have extensive experience and a staff of smart, interested people who will help you find or develop the best sample prep. The equipment is top notch.
Their website is www.struers.com. Here in the Cleveland area of the US Katie Myers is your best bet. You'll find them overseas as well. Note, I don't work for them, own stock in the company or even polish metals anymore, but as far as I'm concerned they are the go to people.
Frank
-----Original Message----- X-from: wolpensinger-at-isfh.de [mailto:wolpensinger-at-isfh.de] Sent: Tuesday, November 15, 2011 6:38 AM To: Frank Karl
Hi all,
Recently we started to prepare cross-section SEM samples. We are using an old Buehler Ecomet III grinder.
I'm now looking for a modern precision lapping/polishing machine for SEM.
X-from a former workplace I know the Allied MultiPrep, which I think was wonderful.
Would such a machine be an overshoot for normal SEM preparation?
What other options are there on the market?
Manufacturers are invited to respond off-list.
I'm aware that a very similar question was asked last year. But then it was for TEM (see below).
Thanks in advance,
Bettina
-----------------------
Hi every body,
We have a very old SEM Hitachi HHS-2R which is working fine but, we can not find filaments in Argentina.
Can anyone tell me where can a get some boxes?
Manufacturers are invited to respond off-list. Thanks in advance,
Victor.
-- ________________________________ Víctor Hugo Casco Prof. Titular Biología Molecular y Celular Facultad de Ingeniería (Bioingenieria - Bioinformática) Universidad Nacional de Entre Ríos Tel.: (0343) 497-5100/101 (int 120) FAX: (0343) 497-5100/101 (int 108) Skype: victor_casco C-electrónico: vcasco-at-bioingenieria.edu.ar www.bioingenieria.edu.ar ___________________________________________
Este correo y cualquier archivo anexo son confidenciales y para uso exclusivo de la persona o entidad de destino. Esta comunicacion puede contener informacion protegida por el privilegio de cliente-abogado. Si usted ha recibido este correo por error, equivocacion u omision queda estrictamente prohibido la utilizacion, copia, reimpresion o reenvio del mismo. En tal caso, favor notificar en forma inmediata al remitente
==============================Original Headers============================== 9, 20 -- From vcasco-at-bioingenieria.edu.ar Tue Nov 15 11:36:03 2011 9, 20 -- Received: from mail-yx0-f169.google.com (mail-yx0-f169.google.com [209.85.213.169]) 9, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAFHa2qY026478 9, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2011 11:36:03 -0600 9, 20 -- Received: by yenm10 with SMTP id m10so4735433yen.0 9, 20 -- for {Microscopy-at-microscopy.com} ; Tue, 15 Nov 2011 09:36:02 -0800 (PST) 9, 20 -- MIME-Version: 1.0 9, 20 -- Received: by 10.68.26.39 with SMTP id i7mr37476272pbg.130.1321378562434; Tue, 9, 20 -- 15 Nov 2011 09:36:02 -0800 (PST) 9, 20 -- Received: by 10.143.163.13 with HTTP; Tue, 15 Nov 2011 09:36:02 -0800 (PST) 9, 20 -- In-Reply-To: {CAPMq3nVEA2quVk7SyUmcr-tuzXQE1Lrzi8rOUC-76+u6+2BZ+w-at-mail.gmail.com} 9, 20 -- References: {CAPMq3nVEA2quVk7SyUmcr-tuzXQE1Lrzi8rOUC-76+u6+2BZ+w-at-mail.gmail.com} 9, 20 -- Date: Tue, 15 Nov 2011 14:36:02 -0300 9, 20 -- Message-ID: {CAPMq3nW3ekNgZAgCZ2YLC23b1Wh_--n7yh7BXRXNhfY+JvVFCw-at-mail.gmail.com} 9, 20 -- Subject: Fwd: filament for SEM 9, 20 -- From: =?ISO-8859-1?B?RHIuIFbtY3RvciBILiBDYXNjbyAoRkktVU5FUik=?= {vcasco-at-bioingenieria.edu.ar} 9, 20 -- To: Microscopy-at-microscopy.com 9, 20 -- Content-Type: text/plain; charset=ISO-8859-1 9, 20 -- Content-Transfer-Encoding: 8bit 9, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAFHa2qY026478 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jzheng-at-uci.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: jzheng-at-uci.edu Name: Jian-Guo Zheng
Organization: University of California, Irvine
Title-Subject: [Filtered] Job open now: Senior Development Engineer, Materials Characterization Facility, University of California, Irvine
Message: Senior Development Engineer, Materials Characterization Facility, University of California, Irvine
Work with the Director to manage the day-to-day operations of UC IrvineÂ’s Materials Characterization Facility, the central campus user facility for electron microscopy, X-ray scattering, scanning probe, and soft materials characterization instrumentation. Located in the new Calit2 Building and the Engineering Tower, the Facility assists over one hundred student users, over 40 faculty, and many outside academic users and corporations in carrying out cutting-edge research in chemistry, materials science, physics, engineering, biology, and medicine. The successful applicant must be an XRD expert and will have direct responsibility for at least three XRDs, one scanning electron microscope, and auxiliary equipment. Duties include: serve as lead campus expert on X-ray and scanning electron microscopy analytical techniques; train, supervise, and work directly with users to carry out research; maintain all Facility equipment at peak levels of performance; co-manage all aspects of day-to-day Facility operations; assist in improving and upgrading existing instruments and acquiring new instruments; participate in teaching and outreach activities; promote the Facility on and off campus; perform original research, publish scientific research in peer-reviewed journals, and attend scientific conferences.
For more information and to apply (by January 1, 2012; job# 2011-0720), visit: https://staffing2.hr.uci.edu/CSS_External/CSSPage_Welcome.asp
The Materials Characterization Facility website is http://lexi.eng.uci.edu/
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both kuryu-at-rockefeller.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Electron Microscopist Position Available
Message: Electron Microscopist, The Rockefeller University, NY, NY The Rockefeller University, a premier biomedical research institution, seeks a Research Support Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state of the art electron microscopy support for analysis of a wide variety of biological samples, including virus, bacteria, insects, animal tissue as well as cultured cell and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with two TEMs and one SEM as well as a high pressure freezing and a free substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Specialist will participate in all of the EMRCÂ’s daily operations, including bench work in preparation for transmission and scanning electron microscopy, and maintenance of the center. He/she will be responsible for all parts of sample preparation, maintenance, and operation of EM and other equipment, training users, consulting with scientists on design of experiments and interpretation of results, ordering and receiving supplies, managing chemical waste compliance, administrative support for office duties and center billing, and keeping up with the latest methodology via familiarity with the literature. Master's degree in biology, cell biology, bioengineering or a related field required; Ph.D. preferred. Must have a minimum of 5 years of experience in electron microscopy and have strong communication skills, and the ability to work collaboratively on a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
Our culture *inspiring, collaborative atmosphere *strong social and environmental consciousness *intellectually curious academic environment *14-acre campus setting in NYC
We offer a competitive salary, comprehensive benefits, and a beautiful work environment. To apply to this job, click the following URL, click on 'staff opportunities’ and enter keyword ‘IRC11162’: http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Kunihiro Uryu, Ph.D., Director of Electron Microscopy Resource Center
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both emma.king-at-nottingham.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: emma.king-at-nottingham.ac.uk Name: Emma King
Organization: University of Nottingham, UK
Title-Subject: [Filtered] 3D reconstruction of multiple fluorescently labelled sections
Message: We would like to take serial sections (20 microns thick) of rat spinal cord (up to 1cm long), label them with a bright and specific fluorescent label, acquire images (we can use either a widefield or confocal microscope) and then reconstruct the data in to one, 3D structure.
We have the sectioning, staining and acquisition parameters/options under control, but don't have any software capable of reconstructing the resultant images. Does anyone have any suggestions as to what software to use and where to get it from?
Any help much appreciated.
Cheers, Emma
Advanced Microscopy Unit School of Biomedical Sciences Univeristy of NOttingham
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ressl006-at-umn.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ressl006-at-umn.edu Name: Alice Ressler
Organization: University of Minnesota Characterization Facility
Title-Subject: [Filtered] TEM Specialist position open
Message: The University of Minnesota is seeking an experienced transmission electron microscopist for a position in the College of Science and Engineering Characterization Facility (CharFac), which houses a variety of transmission and scanning electron microscopes, in addition to other advanced materials and biological characterization instruments. The applicant should possess expertise in routine TEM maintenance, high-resolution TEM imaging, analytical (S)TEM with EELS and EDX spectroscopy, specimen preparation, and interpretation of results from TEM images, analyses, and diffraction patterns. The successful candidate must be able to multi-task on a variety of TEM-related activities, both independently and in collaboration with disparate “users” ranging from those seeking the services of an expert microscopist (i.e., clients, usually with minimal TEM understanding) to those seeking training to become independent (and some cases advanced) microscopists. The job includes a substantial teaching component as the CharFac has a long history of training students, post docs, and industrialists to use state-of-the-art materials characterization instrumentation. A Ph.D. or M.S. in materials science, physics, geology, chemistry, chemical engineering, or similar science or engineering discipline is required. A minimum of five years TEM experience for the characterization of solid-state materials is required. In addition to the technical criteria for a professional microscopist, the following attributes are particularly sought: 1. Ability to interface well with industrial personnel, including training, analytical service and research collaboration. 2. Strong interest in (a) hands-on laboratory work; (b) detailed data analysis; and (c) authoring of client reports, application notes and user training materials (i.e., in addition to journal publications). 3. Interest and aptitude for developing new analytical methodologies as well as IT systems within a technical context (e.g., custom data analysis algorithms). 4. Some experience with aberration-corrected (S)TEMs. Please submit a letter of interest and curriculum vita to: Joel Overlander {charfac-at-umn.edu} and please address your letter as follows: TEM Specialist Search Committee 12 Shepherd Labs Characterization Facility, University of Minnesota 100 Union St. SE Minneapolis, MN 55455
Or you may apply online via the following link: University of Minnesota TEM Position
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both thomas.burrage-at-hq.dhs.gov as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: thomas.burrage-at-hq.dhs.gov Name: Thomas Burrage
Organization: Plum Island Animal Disease Center
Title-Subject: [Filtered] Fixation of liposomes Message: Microscopists,
Can anyone suggest a good protocol for fixing and preserving anionic liposomes for thin section analysis. There were some good liposome studies in the 80's with osmium and tannic acid but I can't get the original papers.
Thanks for your input, Best regards,
Tom Burrage Plum Island Animal Disease Center Greenport, New York 11944-0848 e-mail thomas.burrage-at-hq.dhs.gov lab phone 631 323-3277
I have no experience of doing something like this with so many sections (5,000) in fluorescence. The problems are likely to be the same as those faced by people using EM to reconstruct large (relatively speaking) volumes in 3D- namely alignment of the images and distortion caused during sectioning and mounting.
I don't know what sort of resolution you need, nor how to solve how the labelling would be done, but would some form of imaging directly from the tissue block before sections are removed (or milled) be possible? The equivalent is done with block-face scanning EM to solve the same problems.
Something along the lines of: http://onlinelibrary.wiley.com/doi/10.1002/jemt.20491/pdf
Or you could find a way of capturing and mounting the sections all at the same angle without introducing much distortion. Or you can use software to overcome these.
-- Research Support Manager MRC Anatomical Neuropharmacology Unit, Mansfield Road, Oxford, OX1 3TH, United Kingdom. Telephone: 01865 271649 {http://mrcanu.pharm.ox.ac.uk/}
On 19/11/2011 14:02, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 23, 32 -- From ben.micklem-at-pharm.ox.ac.uk Mon Nov 21 07:52:03 2011 23, 32 -- Received: from relay1.mail.ox.ac.uk (relay1.mail.ox.ac.uk [129.67.1.165]) 23, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pALDq3LU020863 23, 32 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2011 07:52:03 -0600 23, 32 -- Received: from smtp2.nexus.ox.ac.uk ([163.1.154.136] helo=exht02.ad.oak.ox.ac.uk) 23, 32 -- by relay1.mail.ox.ac.uk with esmtp (Exim 4.75) 23, 32 -- (envelope-from {ben.micklem-at-pharm.ox.ac.uk} ) 23, 32 -- id 1RSUHy-0005jL-4h; Mon, 21 Nov 2011 13:52:02 +0000 23, 32 -- Received: from EXMBX04.ad.oak.ox.ac.uk ([169.254.2.176]) by 23, 32 -- exht02.ad.oak.ox.ac.uk ([163.1.154.53]) with mapi; Mon, 21 Nov 2011 13:52:02 23, 32 -- +0000 23, 32 -- From: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk} 23, 32 -- To: "emma.king-at-nottingham.ac.uk" {emma.king-at-nottingham.ac.uk} , 23, 32 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 23, 32 -- Date: Mon, 21 Nov 2011 13:52:01 +0000 23, 32 -- Subject: Re: [Microscopy] viaWWW: 3D reconstruction of multiple 23, 32 -- fluorescently labelled sections 23, 32 -- Thread-Topic: [Microscopy] viaWWW: 3D reconstruction of multiple 23, 32 -- fluorescently labelled sections 23, 32 -- Thread-Index: AcyoVL7P/QNg9OVJSwqkHonN1Lsx3Q== 23, 32 -- Message-ID: {CAF003EE.18AC4%ben.micklem-at-pharm.ox.ac.uk} 23, 32 -- In-Reply-To: {201111191402.pAJE2npS002081-at-ns.microscopy.com} 23, 32 -- Accept-Language: en-US, en-GB 23, 32 -- Content-Language: en-US 23, 32 -- X-MS-Has-Attach: 23, 32 -- X-MS-TNEF-Correlator: 23, 32 -- user-agent: Microsoft-MacOutlook/14.13.0.110805 23, 32 -- acceptlanguage: en-US, en-GB 23, 32 -- Content-Type: text/plain; charset="us-ascii" 23, 32 -- MIME-Version: 1.0 23, 32 -- Content-Transfer-Encoding: 8bit 23, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pALDq3LU020863 ==============================End of - Headers==============================
Hi Emma, This sounds like a great project! First I strongly recommend that you use a confocal with settings for a small optical section in order to give you enough data for reasonable Z-resolution in your final reconstruction. I typically used 0.5 micrometer steps with a 63X and should have used 0.2 or 0.3. The software I have used most is Imaris from Bitplane. It is robust with useful features for neuro studies and they have good support. The cost is high with all similar software. If you try to use open source software you will spend many hours and days getting it all working for your application.
Best wishes, Larry
On 11/19/2011 6:06 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } emma.king-at-nottingham.ac.uk as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: emma.king-at-nottingham.ac.uk Name: Emma King } } Organization: University of Nottingham, UK } } Title-Subject: [Filtered] 3D reconstruction of multiple fluorescently labelled sections } } Message: We would like to take serial sections (20 microns thick) of rat spinal cord (up to 1cm } long), label them with a bright and specific fluorescent label, acquire images (we can use either a } widefield or confocal microscope) and then reconstruct the data in to one, 3D structure. } } We have the sectioning, staining and acquisition parameters/options under control, but don't have } any software capable of reconstructing the resultant images. Does anyone have any suggestions as to } what software to use and where to get it from? } } Any help much appreciated. } } Cheers, } Emma } } Advanced Microscopy Unit } School of Biomedical Sciences } Univeristy of NOttingham } } Login Host: 128.243.253.102 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Sat Nov 19 07:51:38 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAJDpcvb012926 } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Nov 2011 07:51:38 -0600 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 4D84A444048 } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Nov 2011 07:51:38 -0600 (CST) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id v4CTfqKTUchX for {microscopy-at-microscopy.com} ; } 12, 25 -- Sat, 19 Nov 2011 07:51:34 -0600 (CST) } 12, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 79FEC444038 } 12, 25 -- for {microscopy-at-microscopy.com} ; Sat, 19 Nov 2011 07:51:34 -0600 (CST) } 12, 25 -- Message-ID: {4EC7B466.9070105-at-microscopy.com} } 12, 25 -- Date: Sat, 19 Nov 2011 07:51:34 -0600 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: emma.king-at-nottingham.ac.uk } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: viaWWW: 3D reconstruction of multiple fluorescently labelled sections } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== } . }
-- Larry Ackerman, Specialist Electron Microscopy Lab Manager, Diabetes& Endocrinology Research Center Microscopy Core UCSF, Dept. of Anatomy, Rm S657 513 Parnassus Ave., Box 0452 San Francisco, CA 94143
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bkaugust-at-wisc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bkaugust-at-wisc.edu Name: Ben August
Organization: University of Wisconsin
Title-Subject: [Filtered] lanthanum nitrate in sym-collidine with osmium method issues
Message: Greetings, I’m attempting to follow a protocol that utilizes lanthanum nitrate in a mixture of 1% osmium in a 0.2M sym-collidine buffer. The final lanthanum concentration is 0.2M. To make this mixture, I first made a 2X stock solution of sym-collidine (purchased from EMS) and mixed according to their instructions where the sym-collidine is added along with an aliquot of 2.0N. HCL for the desired pH of 7.75. For a 200 ml sym-collidine kit, I made 100ml at 2x (5.34ml sym-collidine from kit, 5.0ml 2.0N HCL in 89.66 ml dH20). To make the final concentrations of the mixture (for 10ml) I added 5ml of the stock sym-collidine, 0.866 grams of lanthanum nitrate and 5ml of 0.2% aqueous osmium. When I add all the components together, it has a slightly yellow milky appearance. After mixing, the liquid becomes somewhat clear, but there is still a precipitate that settles at the bottom of the glass container. Phosphate buffered glutaraldehyde fixed samples were incubated in this mixture for two hours (after carful rinsing in phosphate buffer followed by 1X collidine buffer after primary fixation). After incubation, the samples were routinely processed for TEM (dehydrated in EtOH, propylene oxide and finally embedded in Epon 812 substitute). If prepared properly, the samples should have a layer of electron dense lanthanum nitrate crystals accumulated on the outside or surfaces of the samples and between come of the cells. Mine do not so I suspect that I am doing something wrong. Can someone that has used this mixture successfully please give me some advice? Thanks in advance, Ben August University of Wisconsin – Electron Microscope Facility.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both emma.king-at-nottingham.ac.uk as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: emma.king-at-nottingham.ac.uk Name: Emma King
Title-Subject: [Filtered] 3D reconstruction of multiple tissue sections
Message: Thank you everyone for your suggestions. There's lots to work with... wish us luck!
Cheers, Em
Dr Emma King Advanced Microscopy Unit Lead School of Biomedical Sciences
Good morning, Dear Ben, very interesting issue: Owing to its relative high electron density and granular aspect La is well visualized and has been used as an ultrastructural tracer (e.g. Roels F, Espeel M, Poggi F et al. Human liver pathology in peroxisomal diseases: a review including novel data. Biochimie 1993; 75: 281-292).
Would you please comment or inform us / me of the original recipe /La-method you have considered to follow ["...attempting to follow a protocol..." ] ? There are some specific (unspecific?) methods for precipitation of material (e.g. Calcium, CaBP's, Glycocalyx, etc) using Lanthanum [Also, interestingly, Lanthanum is used as a trapping agent in Hyperphosphataemia]. I have done some projects with tissue fixed with La+++ added and I know that this might be a little bit tricky [first of all I'm thinking of the lanthanum stuff wasn't {reactive} any more in the "mixed fix"-solution], so to comment: - first of all I would like to follow the steps of our scientific predecessors [cf. REVEL, J. P. & KARNOVSKY, M. J. (1967) Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. Cell Biol. 33, C7-C12, a method which in my experience "functions" well] in terms of their used method.
Best regards,
Wolfgang MUSS, PhD EM-Lab Univ.Inst.Pathology SALK-LKH (Gen. Hosp.) SALZBURG, AUSTRIA
} -----Ursprüngliche Nachricht----- } Von: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Gesendet: Dienstag, 22. November 2011 00:23 } An: Muß Wolfgang } Betreff: [Microscopy] Lanthanum nitrate in sym-collidine with osmium } method issues } } --------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } This Question/Comment was submitted to the Microscopy Listserver using the } WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying } please copy both bkaugust-at-wisc.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } Email: bkaugust-at-wisc.edu } Name: Ben August } Organization: University of Wisconsin } Title-Subject: lanthanum nitrate in sym-collidine } with osmium method issues } Message: } } Greetings, I'm attempting to follow a protocol that utilizes lanthanum } nitrate in a mixture of 1% osmium in a 0.2M sym-collidine buffer. } The final lanthanum concentration is 0.2M. } To make this mixture, I first made a 2X stock solution of sym-collidine } (purchased from EMS) and } mixed according to their instructions where the sym-collidine is added } along with an aliquot of } 2.0N. HCL for the desired pH of 7.75. } For a 200 ml sym-collidine kit, I made 100ml at 2x (5.34ml sym- } collidine from kit, 5.0ml 2.0N HCL in 89.66 ml dH20). } To make the final concentrations of the mixture (for 10ml) I added 5ml } of the stock sym-collidine, } 0.866 grams of lanthanum nitrate and 5ml of 0.2% aqueous osmium. } When I add all the components together, it has a slightly yellow milky } appearance. After mixing, the liquid becomes somewhat clear, but there } is still a precipitate that settles at the bottom of the glass } container. Phosphate buffered glutaraldehyde fixed samples were } incubated in this mixture for two hours (after careful rinsing in } phosphate buffer followed by 1X collidine buffer after primary } fixation). } } After incubation, the samples were routinely processed for TEM } (dehydrated in EtOH, propylene oxide and } finally embedded in Epon 812 substitute). If prepared properly, the } samples should have a layer of } electron dense lanthanum nitrate crystals accumulated on the outside or } surfaces of the samples and } between come [some] of the cells. } Mine do not so I suspect that I am doing something wrong. Can someone } that has used this mixture successfully please give me some advice? } Thanks in advance, } } Ben August } University of Wisconsin - Electron Microscope Facility. } } } Login Host: 128.104.8.32 } ----------------------------------------------------------------------- } ---- } } } } ==============================Original } Headers============================== } 9, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Nov 21 } 17:19:31 2011 } 9, 25 -- Received: from znl.com ([206.69.208.20]) } 9, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id pALNJVsK000821 } 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2011 17:19:31 } -0600 } 9, 25 -- Received: from localhost (localhost [127.0.0.1]) } 9, 25 -- by znl.com (Postfix) with ESMTP id 855F0445C2D } 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2011 17:19:31 } -0600 (CST) } 9, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 9, 25 -- Received: from znl.com ([127.0.0.1]) } 9, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 9, 25 -- with ESMTP id 6BU+11xfTEKe for {microscopy-at-microscopy.com} ; } 9, 25 -- Mon, 21 Nov 2011 17:19:26 -0600 (CST) } 9, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 9, 25 -- by znl.com (Postfix) with ESMTPA id 2D7E6445C22 } 9, 25 -- for {microscopy-at-microscopy.com} ; Mon, 21 Nov 2011 17:19:26 } -0600 (CST) } 9, 25 -- Message-ID: {4ECADC7D.7000406-at-microscopy.com} } 9, 25 -- Date: Mon, 21 Nov 2011 17:19:25 -0600 } 9, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 9, 25 -- Reply-To: bkaugust-at-wisc.edu } 9, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 9, 25 -- MIME-Version: 1.0 } 9, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 25 -- Subject: viaWWW:lanthanum nitrate in sym-collidine with osmium } method issues } 9, 25 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 9, 25 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 9, 40 -- From W.Muss-at-salk.at Tue Nov 22 03:00:48 2011 9, 40 -- Received: from hermes.salk.at (hermes.salk.at [193.170.167.9]) 9, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAM90lTm014313 9, 40 -- for {microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 03:00:48 -0600 9, 40 -- Received: from localhost (localhost [127.0.0.1]) 9, 40 -- by hermes.salk.at (Postfix) with ESMTP id 1F7B8C3881 9, 40 -- for {microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 10:00:46 +0100 (CET) 9, 40 -- X-Virii-Scanned: Kaspersky Antivirus at salk.at 9, 40 -- Received: from hermes.salk.at ([127.0.0.1]) 9, 40 -- by localhost (n1ex218.lks.local [127.0.0.1]) (amavisd-new, port 10024) 9, 40 -- with ESMTP id tg-Ua-3veDdP for {microscopy-at-microscopy.com} ; 9, 40 -- Tue, 22 Nov 2011 10:00:45 +0100 (CET) 9, 40 -- Received: from N1EX198.lks.local (n1ex198.lks.local [192.168.13.198]) 9, 40 -- by hermes.salk.at (Postfix) with ESMTP id 41060C389F 9, 40 -- for {microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 10:00:45 +0100 (CET) 9, 40 -- Received: from n2rz123.lksdom21.lks.local ([192.168.101.123]) 9, 40 -- by N1EX198.lks.local with ESMTP; 22 Nov 2011 10:00:36 +0100 9, 40 -- Received: from N1RZ116.lksdom21.lks.local ([192.168.101.132]) by n2rz123.lksdom21.lks.local with Microsoft SMTPSVC(6.0.3790.4675); 9, 40 -- Tue, 22 Nov 2011 10:00:45 +0100 9, 40 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 9, 40 -- Content-class: urn:content-classes:message 9, 40 -- MIME-Version: 1.0 9, 40 -- Content-Type: text/plain; 9, 40 -- charset="iso-8859-1" 9, 40 -- Subject: Re: [Microscopy] Lanthanum nitrate in sym-collidine with osmium method issues 9, 40 -- Date: Tue, 22 Nov 2011 10:00:43 +0100 9, 40 -- Message-ID: {06B4ED29F824524E98E8AA5BB6407062F33331-at-N1RZ116.lksdom21.lks.local} 9, 40 -- In-Reply-To: {201111212323.pALNNK8N010370-at-ns.microscopy.com} 9, 40 -- X-MS-Has-Attach: 9, 40 -- X-MS-TNEF-Correlator: 9, 40 -- Thread-Topic: Re: [Microscopy] Lanthanum nitrate in sym-collidine with osmium method issues 9, 40 -- Thread-Index: AcyopJVG7ECQZxX9Qbaw9Q4uk2H+gwATWzAQ 9, 40 -- References: {201111212323.pALNNK8N010370-at-ns.microscopy.com} 9, 40 -- From: =?iso-8859-1?Q?Mu=DF_Wolfgang?= {W.Muss-at-salk.at} 9, 40 -- To: {microscopy-at-microscopy.com} 9, 40 -- Cc: {bkaugust-at-wisc.edu} 9, 40 -- X-OriginalArrivalTime: 22 Nov 2011 09:00:45.0205 (UTC) FILETIME=[384EE450:01CCA8F5] 9, 40 -- X-Scanned-By: SALK-Content-Filter 9, 40 -- Content-Transfer-Encoding: 8bit 9, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAM90lTm014313 ==============================End of - Headers==============================
We use Perkin Elmer's Volocity 3D visualisation software for this, but it is expensive. Others may use Fuji or ImageJ 3D plug-ins which are free to use and the confocal list-server replies have mentioned a few of those options.
Freebie Fuji's not really any different to Freebie ImageJ for 3D work as far as I can see, and as you'd probably use a 3D ImageJ plug-in like Deconvolution Labs so you may as well stick to ImageJ if you find a suitable ImageJ 3D plug-in. Fuji's integral strength seems to be more stitching together 2D images from motorised XY stage raster scans to recreate whole tissue sections [which Photoshop CS4/5's Photomerge can also do well provided there's overlap]. ImageJ plug-in Deconvolution Labs is quite impressive for 3D rendering, although you might have to follow on-line help to avoid the persistent memory crashes. It's PSF calculations seems similar to Volocitys in that you don't have to input much to get going. The only thing I'm sure about is adding together images to get the z-sequence - if there's no ImageJ freebie for this I suppose any basic video editing package should be able do this, such as PC Pro's A listed Sony Vegas Movie Studio HD Platinum Suite 11 [£50]. Shouldn't be a problem though other than you need to input your z distance between sections/optical slices.
Recreating 3D z stacks is very memory intensive and both freeware ImageJ and expensive Volocity can struggle with frequent crashes due to system memory problems. With Volocity that’s hopefully overcome with Windows 7 64-bit, loads of system RAM memory [8Gb] and a fast 3D gaming card, as the codes optimised for that - plus Volocity's strength has always been Improvisions/Perkin Elmers help desk, where the answer to your problem is generally minutes away [hence the reason I suppose why Volocity costs serious money].
Although Volocity is expensive it's possible someone has a licence for the program within the university, so it would be worth checking that out [Perkin Elmer support who produce Volocity can advise]. If you find a Volocity workstation, contact Perkin Elmer's Volocity support regarding your spinal chords as they will be able offer advice on this. I've actually not tried adding together single Z slices or multiple z stacks from multiple sections using Volocity, as here we always create z-stacks from one specimen and that’s done automatically by our acquisition software with a z motorised focus and saved as a single Volocity compatible file [it can read most files a microscope PC is likely to create]. Plus our Volocity version is out of date and the latest may do more [we hope to upgrade to v6.0 this year]. It should be pretty easy to add folders of images or image sequences to the library though [it's all drag and drop].
I only mention Volocity as we have the software, there's also suitable 3D plugins for other commercial packages like MetaMorph, Image Pro Plus, Imaris and Huygens Software (SVI) to name a few, so check if nearby colleagues in your university have these as well. Even if you end up using imageJ 3D plugins, it's useful to see how the result compares with commercial 3D visualisation and restoration software.
X-from our point of view the only thing Volocity lacks is a neurite outgrowth tree measuring app, although our MetaMorph Offline has a Neurite Outgrowth module and there's similar versions available as plug-ins for ImageJ [the action of which we can usefully verify with our MetaMorph licence]. Bitplane's Imaris is also very strong in the 3D neurite measurement area with their Filament Tracer software, and that seemed very impressive when demo'd to us recently.
We also have NISElements and its EDF function [extended depth of focus]. The EDF app also takes a wide-field [not confocal] Z stack through a tree like structure [neurone type structures in brains or blood vessels in our case]. This time though it doesn't create a 3D image but analyses each image and selects only the in-focus parts. It then adds all the focussed bits from every image and fits them into a single 2D image - squashing the 3D tree into a single focussed 2D photo of the tree as it were. So you want your 3D tree structure lying across the field of view for this to work well.
If you are lucky enough to have a Volocity licence you probably won't be so interested in ImageJ's versions as Volocity can do most that you would require like deconvolution [Restoration], Quantitation [tracking objects and volume/distance measurements], and Visualisation [4d videos], so others on the Confocal/Microscopy list-servers who use ImageJ for this should be able to advise on any 3D/4D plug-ins like Image5D (that looks like it can volume quantitation), FluoRender or Hypervolume - plus there's plenty of help for ImageJ on-line. We use ImageJ here as well as MetaMorph v7.7 here, but largely for 2D image analysis as there's conveniently no licence restrictions for ImageJ.
As posted on the confocal list-server, there are a few ImageJ courses running at the moment in the UK that may help with your 3D work if you can't access a commercial 3D package at your university [you could ask the ImageJ course organisers whether they will cover this] :
---------------------------------- The University of Leicester's College of Medicine, Biological Sciences and Psychology is running another ImageJ/Fiji image analysis workshop over two days:
'Introduction to ImageJ/Fiji' on Monday 19 December 2011 'Writing macros in ImageJ' on Tuesday 20 December 2011.
Their ImageJ workshops in July this year were heavily oversubscribed and so they are being repeated. As there is a maximum 24 participants, book early if you would like to attend [the fee is £50 per workshop or £80 for both].
Please visit their website: http://www.le.ac.uk/biochem/microscopy/ImageJ2011b.html for more details. ---------------------------------- We are also running an introductory course on ImageJ-Fiji at the Institute of Child Health at UCL in November. for more information and to register go to https://www.ucl.ac.uk/ich/services/lab-services/cmcf/training_courses
Best wishes, Bertrand Bertrand Vernay, PhD Microscopy Senior Research Associate Developmental Biology Unit UCL Institute of Child Health 30 Guilford Street London WC1N 1EH, UK -----------------------------------
*CONFOCAL list-server http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy And just follow the join/leave link
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
} Date: Tue, 22 Nov 2011 09:17:07 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: "Susan C. Van Horn" {susan.vanhorn-at-susnysb.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Tuesday, November 22, } 2011 at 09:16:58 AM. } } realname - Susan C. Van Horn } Email - susan.vanhorn-at-susnysb.edu } ORGANIZATION - SUNY -at- Stony Brook } LOCATION - StonyBrook, New York, USA } SUBJECT_OF_QUESTION - post staining precipitate } QUESTION - We are suddenly having a precipitate problem on our } sections which we suspect is from our uranyl acetate - little flecks } and grainy looking.....is anyone having problems with their UA??? } and where are you purchasing it from??? } thanks } sue } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 24 -- From oshel1pe-at-cmich.edu Tue Nov 22 11:22:44 2011 1, 24 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAMHMiHp024916 1, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 11:22:44 -0600 1, 24 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 1, 24 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pAMH312L006984 1, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 12:03:06 -0500 1, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 22 Nov 1, 24 -- 2011 12:22:41 -0500 1, 24 -- MIME-Version: 1.0 1, 24 -- Message-ID: {a0624080bcaf18ab47ed0-at-[141.209.160.249]} 1, 24 -- Date: Tue, 22 Nov 2011 12:22:39 -0500 1, 24 -- To: {Microscopy-at-microscopy.com} 1, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 24 -- Subject: Fwd: Ask-A-Microscopist - UA problems 1, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 24 -- X-Originating-IP: [141.209.160.249] 1, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 1, 24 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 24 -- X-CanItPRO-Stream: default 1, 24 -- X-Canit-Stats-ID: 03G0h36vi - ba786e71b42e - 20111122 1, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
} Date: Tue, 22 Nov 2011 11:25:49 -0800 } Reply-To: "Susan C. Van Horn" {susan.vanhorn-at-sunysb.edu} } Subject: Ask-A-Microscopist } } realname - Susan C. Van Horn } Email - susan.vanhorn-at-sunysb.edu } ORGANIZATION - SUNY -at- Stony Brook } LOCATION - StonyBrook, New York, USA } SUBJECT_OF_QUESTION - post staining precipitate } QUESTION - evidently my email from last posting was incorrect - } sorry!!! am having a problem with what we believe to be UA precip - } small "flecks" everywhere...we make our UA up in methanol and have } used an old block that stained nicely previously but now has these } flecks everywhere - ruling out anything in the embedding } process....we have tried different lots of UA and was wondering if } anyone has had similar problems or knows if the formula has } changed!!! } thanks so much!!! } sue } -- *************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
==============================Original Headers============================== 1, 24 -- From oshel1pe-at-cmich.edu Tue Nov 22 13:37:55 2011 1, 24 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAMJbtO4011406 1, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 13:37:55 -0600 1, 24 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 1, 24 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pAMJIIJ3012237 1, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 14:18:18 -0500 1, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 22 Nov 1, 24 -- 2011 14:37:54 -0500 1, 24 -- MIME-Version: 1.0 1, 24 -- Message-ID: {a0624080dcaf1aa50e774-at-[141.209.160.249]} 1, 24 -- Date: Tue, 22 Nov 2011 14:37:52 -0500 1, 24 -- To: {Microscopy-at-microscopy.com} 1, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 24 -- Subject: Uranyl acetate problem - corrected 1, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 24 -- X-Originating-IP: [141.209.160.249] 1, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 1, 24 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 24 -- X-CanItPRO-Stream: default 1, 24 -- X-Canit-Stats-ID: 03G0jiiRA - 145de9a3dad8 - 20111122 1, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================
I come back to Tom's mail on the preparation of liposomes for TEM. Sorry for only answering today - a bit late.
} Title-Subject: [Filtered] Fixation of liposomes } Message: Microscopists, } } Can anyone suggest a good protocol for fixing and preserving anionic } liposomes for thin section } analysis. There were some good liposome studies in the 80's with osmium and } tannic acid but I can't } get the original papers. } } Thanks for your input, } Best regards, } } Tom Burrage } Plum Island Animal Disease Center } Greenport, New York 11944-0848
Before considering any studies on these liposomes by thin sectioning, I would spend some time exploring the "other" methods which have shown great potential in analysing liposomes ... 1. Cryo-TEM of suspensions, 2. freeze-fracture/-etching, 3. negative staining (preferably on Carbon-coated grids), and only as 4th, I would go the way by fixation and dehydration and embedding. Even then, I would try to find a colleague who has CRYO in his lab: cryo-fixation and freeze-substitution-fixation plus embedding has a number of advantages (but also some con's, I know).
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
==============================Original Headers============================== 7, 25 -- From reinhard.rachel-at-biologie.uni-regensburg.de Tue Nov 22 13:41:20 2011 7, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAMJfK9X016932 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 13:41:20 -0600 7, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 7, 25 -- by localhost (Postfix) with SMTP id C12A836FB 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 20:41:16 +0100 (CET) 7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 954C63394 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 22 Nov 2011 20:41:16 +0100 (CET) 7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 25 -- with Novell_GroupWise; Tue, 22 Nov 2011 20:41:54 +0100 7, 25 -- Message-Id: {4ECC08ED020000540002FAB1-at-gwsmtp1.uni-regensburg.de} 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 12.0.0 Beta 7, 25 -- Date: Tue, 22 Nov 2011 20:41:17 +0100 7, 25 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 7, 25 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 7, 25 -- Subject: Fixation of liposomes 7, 25 -- References: {201111192242.pAJMgp1O025468-at-ns.microscopy.com} 7, 25 -- In-Reply-To: {201111192242.pAJMgp1O025468-at-ns.microscopy.com} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=US-ASCII 7, 25 -- Content-Disposition: inline 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAMJfK9X016932 ==============================End of - Headers==============================
A friend of mine wrote a fine research paper back in 2001 in Tissue and Cell 33(6): 562-569, titled: Sites of Lanthanum Occlusion in the Testis of the Crayfish Procambrus paeninsulanus (Crustacea: Cambaridae), where she used lanthanum to trace tight junctions.
Her procedure was as follows: Fix tissue in the presence of 2% lanthanum nitrate for 2 hours in aldehyde fixative (she used Karnovsky's). Replace the fixative with a 0.03M NaOH/PBS solution (Buckland-Nicks and Chia, 1986) for 30 minutes in order to facilitate lanthanum precipitation. Rinse tissue several times in PBS, then post-fix tissue in 1% osmium tetroxide/PBS with or without 1.5% potassium ferricyanide. She also experimented with adding additional lanthanum nitrate at this step.
Buckland-Nicks, J. and Chia, F. S. 1986. Fine structure of Sertoli cells in three marine snails with a discussion on the functional morphology of Sertoli cells in general. Cell Tissue Research 245: 305-313, 1986.
I haven't repeated her work, but her photos always impressed me, and she does good work. I hope this is of help.
Ed
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119
==============================Original Headers============================== 10, 39 -- From ehaller-at-health.usf.edu Wed Nov 23 07:12:10 2011 10, 39 -- Received: from hscantispam.health.usf.edu (hscantispam.hsc.usf.edu [131.247.67.45]) 10, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pANDC93R002902 10, 39 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Nov 2011 07:12:10 -0600 10, 39 -- X-ASG-Debug-ID: 1322053929-045fa8683501fa0001-1DjkGe 10, 39 -- Received: from EXCH06.hscnet.hsc.usf.edu ([10.119.4.46]) by hscantispam.health.usf.edu with ESMTP id EziOBblFqJoAOaBX; Wed, 23 Nov 2011 08:12:09 -0500 (EST) 10, 39 -- X-Barracuda-Envelope-From: ehaller-at-health.usf.edu 10, 39 -- Received: from EXCH03.hscnet.hsc.usf.edu ([fe80::94db:1b22:fb18:d0b4]) by 10, 39 -- EXCH06.hscnet.hsc.usf.edu ([fe80::3c35:5b92:def1:37f9%14]) with mapi id 10, 39 -- 14.01.0339.001; Wed, 23 Nov 2011 08:12:08 -0500 10, 39 -- From: "Haller, Edward" {ehaller-at-health.usf.edu} 10, 39 -- X-Barracuda-Apparent-Source-IP: fe80::94db:1b22:fb18:d0b4 10, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} , 10, 39 -- "bkaugust-at-wisc.edu" {bkaugust-at-wisc.edu} 10, 39 -- Subject: Lanthanum Nitrate 10, 39 -- Thread-Topic: Lanthanum Nitrate 10, 39 -- X-ASG-Orig-Subj: Lanthanum Nitrate 10, 39 -- Thread-Index: Acyp4Ts6c4jWAg47RC6vut9UJMNbnA== 10, 39 -- Date: Wed, 23 Nov 2011 13:12:08 +0000 10, 39 -- Message-ID: {89AF5EEF4C4B8943A8D091D28859F7C82A38515C-at-EXCH03.hscnet.hsc.usf.edu} 10, 39 -- Accept-Language: en-US 10, 39 -- Content-Language: en-US 10, 39 -- X-MS-Has-Attach: 10, 39 -- X-MS-TNEF-Correlator: 10, 39 -- x-originating-ip: [10.119.4.53] 10, 39 -- Content-Type: text/plain; charset="us-ascii" 10, 39 -- MIME-Version: 1.0 10, 39 -- X-Barracuda-Connect: UNKNOWN[10.119.4.46] 10, 39 -- X-Barracuda-Start-Time: 1322053929 10, 39 -- X-Barracuda-URL: http://hscantispam.health.usf.edu:8000/cgi-mod/mark.cgi 10, 39 -- X-Virus-Scanned: by bsmtpd at health.usf.edu 10, 39 -- X-Barracuda-Spam-Score: 0.00 10, 39 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests= 10, 39 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.2.81069 10, 39 -- Rule breakdown below 10, 39 -- pts rule name description 10, 39 -- ---- ---------------------- -------------------------------------------------- 10, 39 -- Content-Transfer-Encoding: 8bit 10, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pANDC93R002902 ==============================End of - Headers==============================
Hello all- We just experienced a rather unusual issue for the second time and now we'd like to investigate it further. We have a Cameca SX100 using a LaB6 source and equipped with a Gatan MonoCL3 cathodoluminescence imaging system. During CL acquisition (during the last two attempts), the gun vacuum inexplicably increases (ion pump shuts off, no vacuum reading in gun), shutting off the beam and forcing us to open the gun valve to position 3 so that the gun system can get back down to the 10^-6 Pa range. During this time, the vacuum in the chamber does not seem to change and remains at the 10^-4 Pa level -- so we don't think that its a problem with the CL mirror arm. It seems to occur when the Digiscan system takes over control of the probe. Does anyone have a similar experience or thoughts on why this would occur? Any helps appreciated! -Dan
-- Dan Ruscitto, Ph.D.
Laboratory Manager 1W13 Jonsson-Rowland Science Center Earth& Environmental Sciences Rensselaer Polytechnic Institute 110 8th St. Troy NY 12180 Ph: 518-276-2372 Fax: 518-276-2012
==============================Original Headers============================== 4, 23 -- From ruscid2-at-rpi.edu Wed Nov 23 09:58:16 2011 4, 23 -- Received: from smtp8.server.rpi.edu (smtp8.server.rpi.edu [128.113.2.228]) 4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pANFwGU5023062 4, 23 -- for {microscopy-at-microscopy.com} ; Wed, 23 Nov 2011 09:58:16 -0600 4, 23 -- Received: from [128.113.7.205] (wraith-37.dynamic2.rpi.edu [128.113.7.205]) 4, 23 -- (authenticated bits=0) 4, 23 -- by smtp8.server.rpi.edu (8.13.1/8.13.1) with ESMTP id pANFwE9U006996 4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); 4, 23 -- Wed, 23 Nov 2011 10:58:15 -0500 4, 23 -- Message-ID: {4ECD1813.9050403-at-rpi.edu} 4, 23 -- Date: Wed, 23 Nov 2011 10:58:11 -0500 4, 23 -- From: Dan Ruscitto {ruscid2-at-rpi.edu} 4, 23 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:8.0) Gecko/20111105 Thunderbird/8.0 4, 23 -- MIME-Version: 1.0 4, 23 -- To: sx50-users-at-lists.wisc.edu, microscopy-at-microscopy.com 4, 23 -- Subject: Unexplained Gun Vacuum 'Burp' on Cameca SX100 with Gatan MonoCL 4, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 23 -- Content-Transfer-Encoding: 7bit 4, 23 -- X-Bayes-Prob: 0.0001 (Score 0) 4, 23 -- X-RPI-SA-Score: 1.40 (*) [Hold at 12.00] RATWARE_GECKO_BUILD,22490(-25) 4, 23 -- X-CanItPRO-Stream: outgoing 4, 23 -- X-Canit-Stats-ID: Bayes signature not available 4, 23 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 128.113.2.228 ==============================End of - Headers==============================
Hello Dan any chance that the ion pump might be at the end of its lifetime? I had this problem at my LaB6-SEM just some weeks ago... The pump had not been able to getter all the gas from the hot cathode environment after ca. 30 minutes of work and that accelerated like an avalanche, the pump finally becoming very hot...
Best regards, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 23.11.11 17:02, schrieb ruscid2-at-rpi.edu: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello all- } We just experienced a rather unusual issue for the second time and } now we'd like to investigate it further. We have a Cameca SX100 using a } LaB6 source and equipped with a Gatan MonoCL3 cathodoluminescence } imaging system. During CL acquisition (during the last two attempts), } the gun vacuum inexplicably increases (ion pump shuts off, no vacuum } reading in gun), shutting off the beam and forcing us to open the gun } valve to position 3 so that the gun system can get back down to the } 10^-6 Pa range. During this time, the vacuum in the chamber does not } seem to change and remains at the 10^-4 Pa level -- so we don't think } that its a problem with the CL mirror arm. It seems to occur when the } Digiscan system takes over control of the probe. Does anyone have a } similar experience or thoughts on why this would occur? Any helps } appreciated! } -Dan }
==============================Original Headers============================== 7, 23 -- From stefan.diller-at-t-online.de Wed Nov 23 10:10:28 2011 7, 23 -- Received: from mailout08.t-online.de (mailout08.t-online.de [194.25.134.20]) 7, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pANGASJS007552 7, 23 -- for {microscopy-at-microscopy.com} ; Wed, 23 Nov 2011 10:10:28 -0600 7, 23 -- Received: from fwd21.aul.t-online.de (fwd21.aul.t-online.de ) 7, 23 -- by mailout08.t-online.de with smtp 7, 23 -- id 1RTFP0-0004EV-PZ; Wed, 23 Nov 2011 17:10:26 +0100 7, 23 -- Received: from mac-pro.local (rAv086Zvrhnhkypzd5mO8-wJwI3IgK1qDsMilSqwACIevjFJHdPlNfJhHmOf8+jQU4-at-[80.17.62.163]) by fwd21.t-online.de 7, 23 -- with esmtp id 1RTFOx-1iIVA80; Wed, 23 Nov 2011 17:10:23 +0100 7, 23 -- Message-ID: {4ECD1AEE.809-at-t-online.de} 7, 23 -- Date: Wed, 23 Nov 2011 17:10:22 +0100 7, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} 7, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:8.0) Gecko/20111105 Thunderbird/8.0 7, 23 -- MIME-Version: 1.0 7, 23 -- To: microscopy-at-microscopy.com 7, 23 -- Subject: Re: [Microscopy] Unexplained Gun Vacuum 'Burp' on Cameca SX100 with 7, 23 -- Gatan MonoCL 7, 23 -- References: {201111231602.pANG21AC029022-at-ns.microscopy.com} 7, 23 -- In-Reply-To: {201111231602.pANG21AC029022-at-ns.microscopy.com} 7, 23 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 23 -- Content-Transfer-Encoding: 7bit 7, 23 -- X-ID: rAv086Zvrhnhkypzd5mO8-wJwI3IgK1qDsMilSqwACIevjFJHdPlNfJhHmOf8+jQU4 7, 23 -- X-TOI-MSGID: 57d62b79-b64f-4db9-ac18-f4e1ae96bca7 ==============================End of - Headers==============================
I am interested to hear from anyone who has had TEM CCD camera fail in recent years. Ours has failed multiple times over the 5 years. The first 2 times a cause was identified and the camera replaced. Since then failures have resulted in replacement of the camera and more recently replacement of component 'boards'.
Symptoms: bands of interference across the live image leading to rapid loss of signal; no output signal (histogram has flatlined); camera cannot be detected in device manager. Latest replacement of boards resulted in the camera being detected in the device manager but still no signal from the camera. Latest suggestion is to replace the digitizer.
Supplier response: Our service agent is assisting to achieve a resolution but no useful information as to the cause of the failure has been forthcoming from the supplier. The only information forwarded to us verbally was "the boards were fried".
Any suggestions?
Many thanks Naomi
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 12, 25 -- From prvs=13082ffacf=Naomi_McCallum-at-health.qld.gov.au Wed Nov 23 17:42:54 2011 12, 25 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pANNgq49004645 12, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 23 Nov 2011 17:42:53 -0600 12, 25 -- Received: from gwd-mail05.remote.health.qld.gov.au (gwd-mail05.remote.health.qld.gov.au [192.168.3.52]) 12, 25 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id pANNgkDG006227 12, 25 -- for {Microscopy-at-microscopy.com} ; Thu, 24 Nov 2011 09:42:46 +1000 12, 25 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 12, 25 -- gwd-mail05.remote.health.qld.gov.au (Queensland Health SMTP Server) 12, 25 -- with ESMTP id 12, 25 -- {Ta0e15d758cc0a80334628-at-gwd-mail05.remote.health.qld.gov.au} for 12, 25 -- {Microscopy-at-microscopy.com} ; Thu, 24 Nov 2011 09:42:45 +1000 12, 25 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 12, 25 -- Novell_GroupWise; Thu, 24 Nov 2011 09:42:45 +1000 12, 25 -- Message-Id: {4ECE1189.88AE.00AA.0-at-health.qld.gov.au} 12, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 12, 25 -- Date: Thu, 24 Nov 2011 09:42:41 +1000 12, 25 -- From: "Naomi McCallum" {Naomi_McCallum-at-health.qld.gov.au} 12, 25 -- To: {Microscopy-at-microscopy.com} 12, 25 -- Subject: TEM CCD camera failure 12, 25 -- Mime-Version: 1.0 12, 25 -- Content-Type: text/plain; charset="us-ascii" 12, 25 -- Content-Disposition: inline 12, 25 -- Content-Transfer-Encoding: 8bit 12, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pANNgq49004645 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hchen3-at-unl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hchen3-at-unl.edu Name: Han Chen
Organization: University of Nebraska Lincoln
Title-Subject: [Filtered] TEM Negative staining HIV particles, a safety question.
Message: We are BL1 level multiple disciplines users facility. Now, there will be a user to do fixed HIV samples for negative staining. Some users feel uncomfortable to hear this. As I know, fixed HIV samples should be OK in our facility. Does anybody have suggestions that we need to fellow to handle this case? I have checked our University and NIH website, cannot find useful information. If you have a safety protocol for this situation, could you share it with me? Any suggetion is appreciated.
To my knowledge all studies ever conducted has show that HIV is inactivated by standard fixation methods. In fact, fixation as low as 0.2% Glutaraldehyde is considered to be effective.
paul hazelton -- Paul R. Hazelton, PhD Department of Medical Microbiology Head of Electron Microscopy Lab Director, Viral Gastroenteritis Study Group University of Manitoba 511 Basic Medical Sciences Building 745 Bannatyne Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 22 -- From paul_hazelton-at-umanitoba.ca Fri Nov 25 08:16:23 2011 5, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAPEGMkZ008339; 5, 22 -- Fri, 25 Nov 2011 08:16:23 -0600 5, 22 -- Received: from [192.168.100.103] (wnpgmb016qw-ds01-216-100.dynamic.mtsallstream.net [207.161.216.100]) 5, 22 -- (user=hazeltn mech=LOGIN bits=0) 5, 22 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id pAPEG9Y7019727; 5, 22 -- Fri, 25 Nov 2011 08:16:10 -0600 (CST) 5, 22 -- Message-ID: {4ECFA32C.8010708-at-umanitoba.ca} 5, 22 -- Date: Fri, 25 Nov 2011 08:16:12 -0600 5, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.0; WOW64; rv:8.0) Gecko/20111105 Thunderbird/8.0 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microscopylistserver-noreply-at-microscopy.com, 5, 22 -- Microscopy Listserver {microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] viaWWW:TEM Negative staining HIV particles, a safety 5, 22 -- question. 5, 22 -- References: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers==============================
I cannot comment specifically on the amount of fixative required to inactivate HIV, but I would make sure that you know precisely what the researcher means by the term fixation. Fixation is a rather nebulous concept and can mean very different things to different people (or even different things to the same people doing different procedures, eg fixation for immunofluorescence vs fixation for EM). They and you need to know that their treatment (chemicals, concentration and time) is sufficient to inactivate all of the virus.
Matthew
Dr. Matthew J Hannah Lead Electron Microscopist Virus Reference Department Health Protection Agency 61 Colindale Avenue London NW9 5EQ tel: 020 8327 6212
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: 25 November 2011 14:23 To: Matthew Hannah
Han
To my knowledge all studies ever conducted has show that HIV is inactivated by standard fixation methods. In fact, fixation as low as 0.2% Glutaraldehyde is considered to be effective.
paul hazelton -- Paul R. Hazelton, PhD Department of Medical Microbiology Head of Electron Microscopy Lab Director, Viral Gastroenteritis Study Group University of Manitoba 511 Basic Medical Sciences Building 745 Bannatyne Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 22 -- From paul_hazelton-at-umanitoba.ca Fri Nov 25 08:16:23 2011 5, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAPEGMkZ008339; 5, 22 -- Fri, 25 Nov 2011 08:16:23 -0600 5, 22 -- Received: from [192.168.100.103] (wnpgmb016qw-ds01-216-100.dynamic.mtsallstream.net [207.161.216.100]) 5, 22 -- (user=hazeltn mech=LOGIN bits=0) 5, 22 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id pAPEG9Y7019727; 5, 22 -- Fri, 25 Nov 2011 08:16:10 -0600 (CST) 5, 22 -- Message-ID: {4ECFA32C.8010708-at-umanitoba.ca} 5, 22 -- Date: Fri, 25 Nov 2011 08:16:12 -0600 5, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.0; WOW64; rv:8.0) Gecko/20111105 Thunderbird/8.0 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microscopylistserver-noreply-at-microscopy.com, 5, 22 -- Microscopy Listserver {microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] viaWWW:TEM Negative staining HIV particles, a safety 5, 22 -- question. 5, 22 -- References: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers============================== ----------------------------------------- ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk **************************************************************************
I have no experience working with viral samples, but while recently reading some books on EM, I came upon a chapter that treated fixation of virus, and safety. I don’t remember exactly what was the conclusion, but I sure remember one thing, that someone managed to rescue viral particles (some, not all of them) from already fixed and embedded sample for standard (I think) EM. So, please check the literature, there should be enough papers on HIV in EM and do contact the authors of the papers. Unfortunately, I can’t tell you the book title, because I was checking quite a few (all borrowed) and I don’t remember in which one I read it.
Hope this helps,
Josif
Dr. Josif Mircheski ____________________________________________________________________________ ___ Instituto de Biomedicina de Sevilla (IBIS), Lab 108 Campus del Hospital Universitario Virgen del Rocio/CSIC/Univ. de Sevilla Avda. Manuel Siurot S/Nº 41013 Sevilla Spain
Biosafety issues are not a subject for casual discussion on a listserver, they are to be addressed by the relevant institutional biosafety committee.
For Han, I suggest he stop the researcher using the HIV until an approved IBC protocol is in place. This protocol should address how the virus will be handled before it gets to the EM lab, how it will be handled in the EM lab and how it will be safely disposed of once it has been imaged.
The protocol should address suitable labeling of all materials to be used in the lab, warning signs outside and inside the lab, and relevant personal protective equipment.
The biosafetly committee is a group of experts who will understand the safety issues of handling biological hazards and will offer the best advice for handling the virus.
The other users have every right to be concerned by potential exposure to virus until there is an approved IBC protocol in place.
The protocol should also address issues such as how the TEM specimen holder will be sterilized after use so that it can be safely handled by the other users. Again, the safety issue itself may not be important (if the virus is chemically fixed and has been irradiated), but the safety of all users has to be addressed first.
An approved IBC protocol should be in place for any lab that is handing viral, bacterial and fungal pathogens: for human cell lines and human bodily fluids. If the lab is routinely handling unfixed human materials it is also advisable for the staff to be vaccinated against the hepatitis b virus.
Failure to adhere to approved protocols (or failure to even apply for them) can result in loss of NIH funding and law suits.
Regards,
Paul Webster.
House Research Institute 2100 W. 3rd St. Los Angeles, CA 90057, USA.
I thought this was so important an issue that I am willing to bear the Thanksgiving "out of office" junk messages that await - does anyone unsubscribe when the go away from the office?
-----Original Message----- X-from: Matthew.Hannah-at-hpa.org.uk [mailto:Matthew.Hannah-at-hpa.org.uk] Sent: Fri 11/25/2011 9:11 AM To: Webster, Paul
Hi Han
I cannot comment specifically on the amount of fixative required to inactivate HIV, but I would make sure that you know precisely what the researcher means by the term fixation. Fixation is a rather nebulous concept and can mean very different things to different people (or even different things to the same people doing different procedures, eg fixation for immunofluorescence vs fixation for EM). They and you need to know that their treatment (chemicals, concentration and time) is sufficient to inactivate all of the virus.
Matthew
Dr. Matthew J Hannah Lead Electron Microscopist Virus Reference Department Health Protection Agency 61 Colindale Avenue London NW9 5EQ tel: 020 8327 6212
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: 25 November 2011 14:23 To: Matthew Hannah
Han
To my knowledge all studies ever conducted has show that HIV is inactivated by standard fixation methods. In fact, fixation as low as 0.2% Glutaraldehyde is considered to be effective.
paul hazelton -- Paul R. Hazelton, PhD Department of Medical Microbiology Head of Electron Microscopy Lab Director, Viral Gastroenteritis Study Group University of Manitoba 511 Basic Medical Sciences Building 745 Bannatyne Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 22 -- From paul_hazelton-at-umanitoba.ca Fri Nov 25 08:16:23 2011 5, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAPEGMkZ008339; 5, 22 -- Fri, 25 Nov 2011 08:16:23 -0600 5, 22 -- Received: from [192.168.100.103] (wnpgmb016qw-ds01-216-100.dynamic.mtsallstream.net [207.161.216.100]) 5, 22 -- (user=hazeltn mech=LOGIN bits=0) 5, 22 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id pAPEG9Y7019727; 5, 22 -- Fri, 25 Nov 2011 08:16:10 -0600 (CST) 5, 22 -- Message-ID: {4ECFA32C.8010708-at-umanitoba.ca} 5, 22 -- Date: Fri, 25 Nov 2011 08:16:12 -0600 5, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.0; WOW64; rv:8.0) Gecko/20111105 Thunderbird/8.0 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microscopylistserver-noreply-at-microscopy.com, 5, 22 -- Microscopy Listserver {microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] viaWWW:TEM Negative staining HIV particles, a safety 5, 22 -- question. 5, 22 -- References: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers============================== ----------------------------------------- ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk **************************************************************************
Han, Several have replied with comments about fixation and safety committee rulings. It is my experience that most virus particles are inactivated in seconds upon fixation. Even BSL-3 and BSL-4 particles are inactivated for one hour in fixative (4% para/2% glut) and then placed in osmium tetroxide vapor to bring them out of containment. My experience is that there is a good deal of information about inactivation of surfaces with various substances, but little to no real information about specific fixatives for EM fixation and analysis. It is possible to do test runs with the fixative on virus particles that were allowed to adhere to coverslips to determine the effectiveness of the fixative on virus inactivation. You could get the PI to do one test with a specific amount of virus solution and treat the coverslip for one hour and then put the washed coverslip into a culture flask to see if there were any remaining viable particles. Anyway, my two cents. I have always found that 1 hour in 4% paraformaldehyde/2% glutaraldehyde is a great inactivating solution for pretty much anything. I typically do this at room temperature. If you are still a little hesitant about the time, double it to 2 hours. Hope this helps, Robert
On Fri, Nov 25, 2011 at 8:54 AM, { microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both } hchen3-at-unl.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: hchen3-at-unl.edu Name: Han Chen } } Organization: University of Nebraska Lincoln } } Title-Subject: [Filtered] TEM Negative staining HIV particles, a safety } question. } } Message: We are BL1 level multiple disciplines users facility. } Now, there will be a user to do fixed HIV samples for negative staining. } Some users feel } uncomfortable to hear this. As I know, fixed HIV samples should be OK in } our facility. Does anybody } have suggestions that we need to fellow to handle this case? I have } checked our University and NIH } website, cannot find useful information. } If you have a safety protocol for this situation, could you share it with } me? Any suggetion is } appreciated. } } Thank you for your help. } } Han } } Login Host: 129.93.135.50 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Nov 25 } 07:45:24 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id pAPDjO2g023427 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2011 } 07:45:24 -0600 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 41D95448882 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2011 } 07:45:24 -0600 (CST) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 10, 25 -- with ESMTP id hU8napeF0Bdh for {microscopy-at-microscopy.com } } ; } 10, 25 -- Fri, 25 Nov 2011 07:45:21 -0600 (CST) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id 66646448872 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2011 } 07:45:21 -0600 (CST) } 10, 25 -- Message-ID: {4ECF9BF0.2060301-at-microscopy.com} } 10, 25 -- Date: Fri, 25 Nov 2011 07:45:20 -0600 } 10, 25 -- From: MicroscopyListserver-NoReply { } microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: hchen3-at-unl.edu } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: [Filtered] viaWWW:TEM Negative staining HIV particles, a safety } question. } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Nov 27 08:42:57 2011 6, 28 -- Received: from znl.com ([206.69.208.20]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAREgv3c017718 6, 28 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 08:42:57 -0600 6, 28 -- Received: from localhost (localhost [127.0.0.1]) 6, 28 -- by znl.com (Postfix) with ESMTP id 3483C44A141 6, 28 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 08:42:57 -0600 (CST) 6, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 6, 28 -- Received: from znl.com ([127.0.0.1]) 6, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 6, 28 -- with ESMTP id V50e5PXYbnD1 for {microscopy-at-microscopy.com} ; 6, 28 -- Sun, 27 Nov 2011 08:42:46 -0600 (CST) 6, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 6, 28 -- by znl.com (Postfix) with ESMTPA id 517C544A12F 6, 28 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 08:42:46 -0600 (CST) 6, 28 -- Message-ID: {4ED24C65.9000900-at-microscopy.com} 6, 28 -- Date: Sun, 27 Nov 2011 08:42:45 -0600 6, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 6, 28 -- Reply-To: Robert Pope {ropope-at-gmail.com} 6, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 28 -- MIME-Version: 1.0 6, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 6, 28 -- Subject: Re: [Microscopy] viaWWW:TEM Negative staining HIV particles, a safety 6, 28 -- question 6, 28 -- References: {201111251354.pAPDsgWT001019-at-ns.microscopy.com} 6, 28 -- In-Reply-To: {201111251354.pAPDsgWT001019-at-ns.microscopy.com} 6, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
From isabecaromell201102-at-yahoo.com.hk Sun Nov 27 09:58:14 2011 Return-Path: {isabecaromell201102-at-yahoo.com.hk} Received: from mail.netrip.mobi (s100.GosakaFL6.vectant.ne.jp [202.215.111.100]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pARFwEIx003214; Sun, 27 Nov 2011 09:58:14 -0600 Received: from User (unknown [46.183.217.253]) by mail.netrip.mobi (Postfix) with ESMTP id 51E7147828E5; Sat, 26 Nov 2011 14:58:43 +0900 (JST) Reply-To: {isabecaromell201101-at-yahoo.com.hk}
Dear all,
My message about obtaining IBC approval for handling pathogens was not aimed at being critical of aldehydes as sterilizing agents, but to notify everyone that NIH-approved procedures for obtaining permissions for handling these agents are absolute.
Formaldehyde and glutaraldehyde are efficient sterilizing agents that have been used as such for many years. However, there is much more to handling pathogens than making sure the agent has been sterilized.
I think that other users will need to know whether the pathogen will be inactivated before it is brought to the EM laboratory, which space will be used, how the grids be disposed of after use, how aerosols will be prevented, and if other users will be notified when someone using the pathogen is working in the shared space?
Submitting an IBC protocol should be an easy process but once it is approved it will put the safety protocols in the hands of the PI and give the head of the EM lab a level of control that will be needed if the people handling the pathogen are not as careful as they should be.
In the case of HIV virus, the risk of cross-infection is very low even if the particles are not inactivated with aldehyde or bleach, but the risk is still there. Check with your IBC before doing anything.
Paul Webster.
House Research Institute 2100 W 3rd. St. Los Angeles CA 90057, USA.
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Sun 11/27/2011 6:47 AM To: Webster, Paul
Han, Several have replied with comments about fixation and safety committee rulings. It is my experience that most virus particles are inactivated in seconds upon fixation. Even BSL-3 and BSL-4 particles are inactivated for one hour in fixative (4% para/2% glut) and then placed in osmium tetroxide vapor to bring them out of containment. My experience is that there is a good deal of information about inactivation of surfaces with various substances, but little to no real information about specific fixatives for EM fixation and analysis. It is possible to do test runs with the fixative on virus particles that were allowed to adhere to coverslips to determine the effectiveness of the fixative on virus inactivation. You could get the PI to do one test with a specific amount of virus solution and treat the coverslip for one hour and then put the washed coverslip into a culture flask to see if there were any remaining viable particles. Anyway, my two cents. I have always found that 1 hour in 4% paraformaldehyde/2% glutaraldehyde is a great inactivating solution for pretty much anything. I typically do this at room temperature. If you are still a little hesitant about the time, double it to 2 hours. Hope this helps, Robert
On Fri, Nov 25, 2011 at 8:54 AM, { microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both } hchen3-at-unl.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: hchen3-at-unl.edu Name: Han Chen } } Organization: University of Nebraska Lincoln } } Title-Subject: [Filtered] TEM Negative staining HIV particles, a safety } question. } } Message: We are BL1 level multiple disciplines users facility. } Now, there will be a user to do fixed HIV samples for negative staining. } Some users feel } uncomfortable to hear this. As I know, fixed HIV samples should be OK in } our facility. Does anybody } have suggestions that we need to fellow to handle this case? I have } checked our University and NIH } website, cannot find useful information. } If you have a safety protocol for this situation, could you share it with } me? Any suggetion is } appreciated. } } Thank you for your help. } } Han } } Login Host: 129.93.135.50 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Nov 25 } 07:45:24 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id pAPDjO2g023427 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2011 } 07:45:24 -0600 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 41D95448882 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2011 } 07:45:24 -0600 (CST) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 10, 25 -- with ESMTP id hU8napeF0Bdh for {microscopy-at-microscopy.com } } ; } 10, 25 -- Fri, 25 Nov 2011 07:45:21 -0600 (CST) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id 66646448872 } 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 25 Nov 2011 } 07:45:21 -0600 (CST) } 10, 25 -- Message-ID: {4ECF9BF0.2060301-at-microscopy.com} } 10, 25 -- Date: Fri, 25 Nov 2011 07:45:20 -0600 } 10, 25 -- From: MicroscopyListserver-NoReply { } microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: hchen3-at-unl.edu } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: [Filtered] viaWWW:TEM Negative staining HIV particles, a safety } question. } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
==============================Original Headers============================== 6, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Nov 27 08:42:57 2011 6, 28 -- Received: from znl.com ([206.69.208.20]) 6, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAREgv3c017718 6, 28 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 08:42:57 -0600 6, 28 -- Received: from localhost (localhost [127.0.0.1]) 6, 28 -- by znl.com (Postfix) with ESMTP id 3483C44A141 6, 28 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 08:42:57 -0600 (CST) 6, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 6, 28 -- Received: from znl.com ([127.0.0.1]) 6, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 6, 28 -- with ESMTP id V50e5PXYbnD1 for {microscopy-at-microscopy.com} ; 6, 28 -- Sun, 27 Nov 2011 08:42:46 -0600 (CST) 6, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 6, 28 -- by znl.com (Postfix) with ESMTPA id 517C544A12F 6, 28 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 08:42:46 -0600 (CST) 6, 28 -- Message-ID: {4ED24C65.9000900-at-microscopy.com} 6, 28 -- Date: Sun, 27 Nov 2011 08:42:45 -0600 6, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 6, 28 -- Reply-To: Robert Pope {ropope-at-gmail.com} 6, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 28 -- MIME-Version: 1.0 6, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 6, 28 -- Subject: Re: [Microscopy] viaWWW:TEM Negative staining HIV particles, a safety 6, 28 -- question 6, 28 -- References: {201111251354.pAPDsgWT001019-at-ns.microscopy.com} 6, 28 -- In-Reply-To: {201111251354.pAPDsgWT001019-at-ns.microscopy.com} 6, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 21, 21 -- From PWebster-at-hei.org Sun Nov 27 14:15:49 2011 21, 21 -- Received: from heimail.hei.org (heimail.hei.org [12.175.189.159]) 21, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pARKFmoh013908 21, 21 -- for {microscopy-at-microscopy.com} ; Sun, 27 Nov 2011 14:15:49 -0600 21, 21 -- Content-class: urn:content-classes:message 21, 21 -- MIME-Version: 1.0 21, 21 -- Content-Type: text/plain; 21, 21 -- charset="iso-8859-1" 21, 21 -- Subject: FW: [Microscopy] Re: viaWWW:TEM Negative staining HIV particles, a safety 21, 21 -- X-MimeOLE: Produced By Microsoft Exchange V6.5 21, 21 -- Date: Sun, 27 Nov 2011 12:15:48 -0800 21, 21 -- Message-ID: {4B5D9B28C1F49548B4D3C429EE0D32E039016F-at-hi0sml1.hei.org} 21, 21 -- X-MS-Has-Attach: 21, 21 -- X-MS-TNEF-Correlator: 21, 21 -- Thread-Topic: [Microscopy] Re: viaWWW:TEM Negative staining HIV particles, a safety 21, 21 -- Thread-Index: AcytE4HSwQsnUjdhSxeKcKfHln2X+AALDTxA 21, 21 -- References: {201111271447.pARElaWd022558-at-ns.microscopy.com} 21, 21 -- From: "Webster, Paul" {PWebster-at-hei.org} 21, 21 -- To: {microscopy-at-microscopy.com} 21, 21 -- Content-Transfer-Encoding: 8bit 21, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pARKFmoh013908 ==============================End of - Headers==============================
I must agree with Paul on this matter. HIV poses real safety issues and working without a precise protocol and strict rules is irresponsible. If someone fixes the virus with aldehydes this should be done in a fume hood with strict safety procedures. Is the fume hood BSL-2? What kind of filter does the hood have? Who has access to the hood? Even after fixation you should not treat it as a BSL-1 material. Let me remind you that one of the principles of BSL-2 is containment, meaning only authorized personal has access to the material and everything is precisely labeled. As Paul said, everybody has the right to work in a secure environment. You must assure that untrained personal never come in contact with this material. Never meaning not even once, by mistake.
X-from: "PWebster-at-hei.org" {PWebster-at-hei.org} To: nizets2-at-yahoo.com Sent: Friday, November 25, 2011 8:02 PM
Dear Han (and everyone else),
Biosafety issues are not a subject for casual discussion on a listserver, they are to be addressed by the relevant institutional biosafety committee.
For Han, I suggest he stop the researcher using the HIV until an approved IBC protocol is in place. This protocol should address how the virus will be handled before it gets to the EM lab, how it will be handled in the EM lab and how it will be safely disposed of once it has been imaged.
The protocol should address suitable labeling of all materials to be used in the lab, warning signs outside and inside the lab, and relevant personal protective equipment.
The biosafetly committee is a group of experts who will understand the safety issues of handling biological hazards and will offer the best advice for handling the virus.
The other users have every right to be concerned by potential exposure to virus until there is an approved IBC protocol in place.
The protocol should also address issues such as how the TEM specimen holder will be sterilized after use so that it can be safely handled by the other users. Again, the safety issue itself may not be important (if the virus is chemically fixed and has been irradiated), but the safety of all users has to be addressed first.
An approved IBC protocol should be in place for any lab that is handing viral, bacterial and fungal pathogens: for human cell lines and human bodily fluids. If the lab is routinely handling unfixed human materials it is also advisable for the staff to be vaccinated against the hepatitis b virus.
Failure to adhere to approved protocols (or failure to even apply for them) can result in loss of NIH funding and law suits.
Regards,
Paul Webster.
House Research Institute 2100 W. 3rd St. Los Angeles, CA 90057, USA.
I thought this was so important an issue that I am willing to bear the Thanksgiving "out of office" junk messages that await - does anyone unsubscribe when the go away from the office?
-----Original Message----- X-from: Matthew.Hannah-at-hpa.org.uk [mailto:Matthew.Hannah-at-hpa.org.uk] Sent: Fri 11/25/2011 9:11 AM To: Webster, Paul
Hi Han
I cannot comment specifically on the amount of fixative required to inactivate HIV, but I would make sure that you know precisely what the researcher means by the term fixation. Fixation is a rather nebulous concept and can mean very different things to different people (or even different things to the same people doing different procedures, eg fixation for immunofluorescence vs fixation for EM). They and you need to know that their treatment (chemicals, concentration and time) is sufficient to inactivate all of the virus.
Matthew
Dr. Matthew J Hannah Lead Electron Microscopist Virus Reference Department Health Protection Agency 61 Colindale Avenue London NW9 5EQ tel: 020 8327 6212
-----Original Message----- X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca] Sent: 25 November 2011 14:23 To: Matthew Hannah
Han
To my knowledge all studies ever conducted has show that HIV is inactivated by standard fixation methods. In fact, fixation as low as 0.2% Glutaraldehyde is considered to be effective.
paul hazelton -- Paul R. Hazelton, PhD Department of Medical Microbiology Head of Electron Microscopy Lab Director, Viral Gastroenteritis Study Group University of Manitoba 511 Basic Medical Sciences Building 745 Bannatyne Avenue Winnipeg, Manitoba, Canada, R3E 3J7 e-mail: paul_hazelton-at-umanitoba.ca paulhazelton-at-mts.net Phone: 204-789-3313 (w); 204-489-6924 (h) Cell: 204-781-6982 Fax: 204-789-3926
==============================Original Headers============================== 5, 22 -- From paul_hazelton-at-umanitoba.ca Fri Nov 25 08:16:23 2011 5, 22 -- Received: from electra.cc.umanitoba.ca (electra.cc.umanitoba.ca [130.179.16.34]) 5, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAPEGMkZ008339; 5, 22 -- Fri, 25 Nov 2011 08:16:23 -0600 5, 22 -- Received: from [192.168.100.103] (wnpgmb016qw-ds01-216-100.dynamic.mtsallstream.net [207.161.216.100]) 5, 22 -- (user=hazeltn mech=LOGIN bits=0) 5, 22 -- by electra.cc.umanitoba.ca (8.14.4/8.14.4) with ESMTP id pAPEG9Y7019727; 5, 22 -- Fri, 25 Nov 2011 08:16:10 -0600 (CST) 5, 22 -- Message-ID: {4ECFA32C.8010708-at-umanitoba.ca} 5, 22 -- Date: Fri, 25 Nov 2011 08:16:12 -0600 5, 22 -- From: paul r hazelton {paul_hazelton-at-umanitoba.ca} 5, 22 -- User-Agent: Mozilla/5.0 (Windows NT 6.0; WOW64; rv:8.0) Gecko/20111105 Thunderbird/8.0 5, 22 -- MIME-Version: 1.0 5, 22 -- To: microscopylistserver-noreply-at-microscopy.com, 5, 22 -- Microscopy Listserver {microscopy-at-microscopy.com} 5, 22 -- Subject: Re: [Microscopy] viaWWW:TEM Negative staining HIV particles, a safety 5, 22 -- question. 5, 22 -- References: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- In-Reply-To: {201111251347.pAPDlIFc025795-at-ns.microscopy.com} 5, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 22 -- Content-Transfer-Encoding: 7bit 5, 22 -- X-DCC-UofM-Metrics: electra; whitelist ==============================End of - Headers============================== ----------------------------------------- ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of the HPA, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses, but please re-sweep any attachments before opening or saving. HTTP://www.HPA.org.uk **************************************************************************
We have leak in the pneumatics system of our tecnai G20 TEM and I would appreciate to receive some clue about how to deal with it. At first we had a clear leak at the manometer, the one where to main pressured air is connected to (indicating normally 5 bar). Now we have replaced the part and this part al least seems ok now but we still hear a hissing sound unter the microscope when we put the pressure at 5 bar. The sound disappears when the pressure drops to 4 bars but the pressure still continue to drop down to atmospheric pressure. I am not sure if there is a more efficient way to check for leaks than unplug all connections are replug them. Actually one technician offered us to use a can of a substance which is supposed to detect leaks but I don't know if it would be hazardous to use in on the pneumatics of a TEM. It is not written on the flask what kind of substance it is. It is a product from Linde and is called TPS 674 (a german product). I give here a link to an example: http://www.gasecenter-shop.de/Propan/Propanzubehoer/Lecksuchspray-125-ml-zum-schnellen-Auffinden-von-Undichtigkeiten::84.html
Any suggestion welcome.
Regards, Stephane now awaiting the massive amount of out-of-office replies (last time 21)
==============================Original Headers============================== 2, 39 -- From nizets2-at-yahoo.com Mon Nov 28 10:02:41 2011 2, 39 -- Received: from nm13-vm0.bullet.mail.sp2.yahoo.com (nm13-vm0.bullet.mail.sp2.yahoo.com [98.139.91.244]) 2, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pASG2fxT005230 2, 39 -- for {microscopy-at-microscopy.com} ; Mon, 28 Nov 2011 10:02:41 -0600 2, 39 -- Received: from [98.139.91.70] by nm13.bullet.mail.sp2.yahoo.com with NNFMP; 28 Nov 2011 16:02:40 -0000 2, 39 -- Received: from [98.139.91.52] by tm10.bullet.mail.sp2.yahoo.com with NNFMP; 28 Nov 2011 16:02:40 -0000 2, 39 -- Received: from [127.0.0.1] by omp1052.mail.sp2.yahoo.com with NNFMP; 28 Nov 2011 16:02:40 -0000 2, 39 -- X-Yahoo-Newman-Property: ymail-3 2, 39 -- X-Yahoo-Newman-Id: 939565.1833.bm-at-omp1052.mail.sp2.yahoo.com 2, 39 -- Received: (qmail 7617 invoked by uid 60001); 28 Nov 2011 16:02:40 -0000 2, 39 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1322496160; bh=mJ/1nwHm7pM/rrPKR+vHFZAnhxWz9n/+PWi27n9zZM8=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=BEMWAgAl9igjkpObuiP9AmtZGD6tRahOyEKfweeCU3Ji5CPUGMz6Nht6GLabsXRKlfI+9Pt7X5Xr9+KVDy/C0gD2Xw86P7z2l5gUMwnVzxMKG4QJqR3A7yfnQ6gw6m6jpwGxZ88mCSp1DGxtFDiDZXq3bVimOSQbkI9D3GwL+Yc= 2, 39 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 39 -- s=s1024; d=yahoo.com; 2, 39 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 2, 39 -- b=YaaglH1hzdYUZtMJX2xM+VDP9qaZavNJXCHhBd4m7AiwEFF2Ukj8iG948wVrlBhPqVPJ/sqm/D/pOz4pdex6AOt/yAsuSypEQdJXhzgUhmFmeHDf3V5017el+c68nYaOZTuHHzMEhaLCpkL5ugdjk93dFm30gT8ZvwqP8h4+W0s=; 2, 39 -- X-YMail-OSG: l6bPjtgVM1l.jAThytxS20MeNJzD7OqQ44cdu2re0U6hQSi 2, 39 -- ykES.8o5LQgWf0gS900ykks9TFwZSNhvY1CE5mx5hJ4ZYZ2gY0VqlmINjLp2 2, 39 -- dEzkGPbv4nYOUH.Yjb.DBn27knKKU_Ayqd4YSvAoma10PFV6D3IP5EoTWDk7 2, 39 -- 0r3UYM_P..0LOGcFCl7E8C_YC0zUGYLI5oAgJv_MVltK8FqKAqYBNLrZtsAg 2, 39 -- hHEAw2IzehCnmID6LFXG_8ALDV0HkMM6qeY_r754KNYtd1oRvKSlNwJUk6zn 2, 39 -- mrffE9g6k3b77GirsuCabZWsrpbOvRq7W2LLBK4mqQTCpAwNpVZQitvD8wg. 2, 39 -- v_dDTPh6eMCTBsEg8J9332ZvrscwctfNWR2rjQg4zB4PQckeGRpTjA__RwPi 2, 39 -- K.bteY9JGvEPTHBmFWo_iiAqiiGXQ0sK3cafgiywJ2FB1K5AyV7MiDDRBASa 2, 39 -- EUn3h2UPxLW6ddmgGQgoxwSv9jYPyWGjQ.F1Lc0pxpETcmp3VRf4bkIXAiWi 2, 39 -- 03gyyf2g2I9YmQFs5AY5pixNZhDO3L7uWfEa1Br67._nMO4eivo85iEC2RDV 2, 39 -- 5C65MNskJPER8mhRY43uf0rC._NmSa7ZKYjJecFrSIsDw_CNybaq_pXEkD4j 2, 39 -- 8r_kAHJA649Z4H28zWObl4b1fz0Er 2, 39 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Mon, 28 Nov 2011 08:02:40 PST 2, 39 -- X-Mailer: YahooMailWebService/0.8.115.331698 2, 39 -- Message-ID: {1322496160.25410.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} 2, 39 -- Date: Mon, 28 Nov 2011 08:02:40 -0800 (PST) 2, 39 -- From: Stephane Nizet {nizets2-at-yahoo.com} 2, 39 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 2, 39 -- Subject: leak in pneumatics (TEM) 2, 39 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 2, 39 -- MIME-Version: 1.0 2, 39 -- Content-Type: text/plain; charset=iso-8859-1 2, 39 -- Content-Transfer-Encoding: 8bit 2, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pASG2fxT005230 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://ns.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ravi.thakkar-at-iitb.ac.in as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: ravi.thakkar-at-iitb.ac.in Name: Ravi Thakkar
Organization: Indian Institute of Technology, Bombay, INDIA.
Title-Subject: [Filtered] Need guidance for Buying new TEM
Message: Dear Listeners,
What are the criterion are taken to be considered for the purchase of new TEM .??
Kindly guide me to purchase new TEM. following are the objective and required features. Objective : Biological and soft samples. Required Features : Cryo Mode operation, FEG, 40-200 KT HT capacity, STEM, EDAX, Bright field with Dark field, Electron Diffraction, 3 D Tomography, EELS (optional).
With Thanks and regards. ------------------------------------------------------------ Ravindra Thakkar Cryo TEM - Central Facility in SAIF, C/O Prof. Jayesh Bellare, Dept. of Chemical Engineering, I.I.T. Bombay, Mumbai - 400076 (INDIA) Tel. : +91-22-25764688/4681 Mo. : +91-9769474636 E-mail : ravi.thakkar-at-iitb.ac.in ------------------------------------------------------------
We have a JEOL JAMP-7830F auger microprobe on campus. It has recently developed a leak around the bellows of the probe current detector.
That system is not under service contract but it does get a lot of use. Does anyone out there happen to have such a system sitting idle from which we could get the PCD assembly?
JEOL is looking into availability of the part, but we would like to open this up to the wider community. You folks have helped out wonderfully in similar circumstances before.
Warren Straszheim
==============================Original Headers============================== 5, 29 -- From wesaia-at-iastate.edu Tue Nov 29 12:27:33 2011 5, 29 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pATIRXFt021194 5, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Nov 2011 12:27:33 -0600 5, 29 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702a.its.iastate.edu [10.10.87.140]) 5, 29 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id pATIRWF5016653; 5, 29 -- Tue, 29 Nov 2011 12:27:32 -0600 5, 29 -- Received: from EXITS711.its.iastate.edu ([10.10.87.144]) by 5, 29 -- EXITS702A.its.iastate.edu ([10.10.87.140]) with mapi; Tue, 29 Nov 2011 5, 29 -- 12:27:32 -0600 5, 29 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 29 -- Date: Tue, 29 Nov 2011 12:27:32 -0600 5, 29 -- Subject: Need PCD assembly or bellows for Auger probe 5, 29 -- Thread-Topic: Need PCD assembly or bellows for Auger probe 5, 29 -- Thread-Index: Acyuv5+5QXpeVoCqTZyXaW7BWHCZQA== 5, 29 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F2905E86A29-at-EXITS711.its.iastate.edu} 5, 29 -- Accept-Language: en-US 5, 29 -- Content-Language: en-US 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- acceptlanguage: en-US 5, 29 -- Content-Type: text/plain; charset="us-ascii" 5, 29 -- MIME-Version: 1.0 5, 29 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.11.29.181814 5, 29 -- X-ISUMailhub-test: Gauge=XIIIIIIII, Probability=18%, Report=' 5, 29 -- RATWARE_RCVD_BONUS_SPC 2.0, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_500_599 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, FROM_NAME_PHRASE 0, NO_URI_FOUND 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0' 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pATIRXFt021194 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman-at-pacific.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman-at-pacific.edu Name: Barbara L. Plowman
Organization: University of the Pacific- Arthur A. Dugoni School of Dentistry
Title-Subject: [Filtered] HIV safety
Message: We did our HIV research in a P3 level laboratory and fixed all HIV- infected cells with 3% glut in cacadylate buffer, post fixed in !% OsO4. And we used 9% bleach to clean up.
It should be possible to find a bellow manufacturer in the USA which could repare yours. We had soon such problemes on UHV STM, Auger, MBE, etc, with wobble stick or other bellowed devices, and in Europe we have several companies which make and can repair such bellows. Of coarse one must dismantle the hole PCD. In the USA you can ask Huntington, MDC, HVA or Lesker, or make a search with "vacuum bellow" as key word. The price may varie much, between a smale manufacturer and a big seller, which will contract out and take its profit margin.
Meanwhile, you can stop the leak with a spray or varnish like "Leak Sealent" sold by SPI for exemple (http://www.2spi.com/catalog/vac/vacleak.shtml).
Hope it helps
Veuillez prendre note de la nouvelle adresse mail.
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
Le 29/11/2011 19:40, wesaia-at-iastate.edu a écrit : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } We have a JEOL JAMP-7830F auger microprobe on campus. It has recently developed a leak around the bellows of the probe current detector. } } That system is not under service contract but it does get a lot of use. Does anyone out there happen to have such a system sitting idle from which we could get the PCD assembly? } } JEOL is looking into availability of the part, but we would like to open this up to the wider community. You folks have helped out wonderfully in similar circumstances before. } } Warren Straszheim } } } ==============================Original Headers============================== } 5, 29 -- From wesaia-at-iastate.edu Tue Nov 29 12:27:33 2011 } 5, 29 -- Received: from mailexch-1.iastate.edu (mailexch-1.iastate.edu [129.186.140.21]) } 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pATIRXFt021194 } 5, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Nov 2011 12:27:33 -0600 } 5, 29 -- Received: from EXITS702.ITS.IASTATE.EDU (exits702a.its.iastate.edu [10.10.87.140]) } 5, 29 -- by mailexch-1.iastate.edu (8.14.4/8.12.10) with ESMTP id pATIRWF5016653; } 5, 29 -- Tue, 29 Nov 2011 12:27:32 -0600 } 5, 29 -- Received: from EXITS711.its.iastate.edu ([10.10.87.144]) by } 5, 29 -- EXITS702A.its.iastate.edu ([10.10.87.140]) with mapi; Tue, 29 Nov 2011 } 5, 29 -- 12:27:32 -0600 } 5, 29 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} } 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 5, 29 -- Date: Tue, 29 Nov 2011 12:27:32 -0600 } 5, 29 -- Subject: Need PCD assembly or bellows for Auger probe } 5, 29 -- Thread-Topic: Need PCD assembly or bellows for Auger probe } 5, 29 -- Thread-Index: Acyuv5+5QXpeVoCqTZyXaW7BWHCZQA== } 5, 29 -- Message-ID: {AA96B6F163D82F479AE1E4CA9000980F2905E86A29-at-EXITS711.its.iastate.edu} } 5, 29 -- Accept-Language: en-US } 5, 29 -- Content-Language: en-US } 5, 29 -- X-MS-Has-Attach: } 5, 29 -- X-MS-TNEF-Correlator: } 5, 29 -- acceptlanguage: en-US } 5, 29 -- Content-Type: text/plain; charset="us-ascii" } 5, 29 -- MIME-Version: 1.0 } 5, 29 -- X-PMX-Version: 5.6.1.2065439, Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.11.29.181814 } 5, 29 -- X-ISUMailhub-test: Gauge=XIIIIIIII, Probability=18%, Report=' } 5, 29 -- RATWARE_RCVD_BONUS_SPC 2.0, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_500_599 0, BODY_SIZE_7000_LESS 0, DATE_TZ_NA 0, FROM_NAME_PHRASE 0, NO_URI_FOUND 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, __HAS_MSGID 0, __MIME_TEXT_ONLY 0, __MIME_VERSION 0, __SANE_MSGID 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0' } 5, 29 -- Content-Transfer-Encoding: 8bit } 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pATIRXFt021194 } ==============================End of - Headers==============================
==============================Original Headers============================== 9, 29 -- From jacques.faerber-at-ipcms.unistra.fr Wed Nov 30 02:40:52 2011 9, 29 -- Received: from mailhost.u-strasbg.fr (mailhost.u-strasbg.fr [130.79.200.153]) 9, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAU8ep8b014404 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 02:40:52 -0600 9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) 9, 29 -- by mailhost.u-strasbg.fr (8.14.3/jtpda-5.5pre1) with ESMTP id pAU8eoQ4028559 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 09:40:50 +0100 (CET) (envelope-from jacques.faerber-at-ipcms.unistra.fr) 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 9, 29 -- (No client certificate requested) 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id BDE484A005 9, 29 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 10:15:52 +0100 (CET) 9, 29 -- Message-ID: {4ED5EC1C.4050303-at-ipcms.u-strasbg.fr} 9, 29 -- Date: Wed, 30 Nov 2011 09:41:00 +0100 9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} 9, 29 -- User-Agent: Mozilla/5.0 (X11; U; Linux i686; en-US; rv:1.9.2.23) Gecko/20110921 Thunderbird/3.1.15 9, 29 -- MIME-Version: 1.0 9, 29 -- To: microscopy-at-microscopy.com 9, 29 -- Subject: Re: [Microscopy] Need PCD assembly or bellows for Auger probe 9, 29 -- References: {201111291840.pATIeTq4005535-at-ns.microscopy.com} 9, 29 -- In-Reply-To: {201111291840.pATIeTq4005535-at-ns.microscopy.com} 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-IPCMS-MailScanner: Found to be clean 9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.unistra.fr 9, 29 -- X-Greylist: Sender IP whitelisted, not delayed by milter-greylist-4.2.5 (mailhost.u-strasbg.fr [130.79.200.153]); Wed, 30 Nov 2011 09:40:50 +0100 (CET) 9, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=TO_NO_BRKTS_PCNT, 9, 29 -- T_FRT_BELOW2,USER_IN_WHITELIST autolearn=disabled version=3.3.1 9, 29 -- X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on mr3.u-strasbg.fr ==============================End of - Headers==============================
This reminds me: another place to look for vacuum parts is Duniway Stockroom: http://www.duniway.com
Phil
} Hi Warren } } It should be possible to find a bellow manufacturer in the USA which } could repare yours. We had soon such problemes on UHV STM, Auger, MBE, } etc, with wobble stick or other bellowed devices, and in Europe we have } several companies which make and can repair such bellows. Of coarse one } must dismantle the hole PCD. } In the USA you can ask Huntington, MDC, HVA or Lesker, or make a search } with "vacuum bellow" as key word. } The price may varie much, between a smale manufacturer and a big seller, } which will contract out and take its profit margin. } } Meanwhile, you can stop the leak with a spray or varnish like "Leak } Sealent" sold by SPI for exemple } (http://www.2spi.com/catalog/vac/vacleak.shtml). } } Hope it helps } } Veuillez prendre note de la nouvelle adresse mail. } } J. Faerber } IPCMS-DSI } Institut de Physique et Chimie des Matériaux de Strasbourg } Département Surfaces et Interfaces } 23, rue de Loess ; BP43 } 67034 Strasbourg CEDEX 2 } France } } Tel 00 33(0)3 88 10 71 01 } Fax 00 33(0)3 88 10 72 48 } E-mail Jacques.Faerber-at-ipcms.unistra.fr } } } Le 29/11/2011 19:40, wesaia-at-iastate.edu a écrit : } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } We have a JEOL JAMP-7830F auger microprobe on } } campus. It has recently developed a leak around } } the bellows of the probe current detector. } } } } That system is not under service contract but } } it does get a lot of use. Does anyone out there } } happen to have such a system sitting idle from } } which we could get the PCD assembly? } } } } JEOL is looking into availability of the part, } } but we would like to open this up to the wider } } community. You folks have helped out } } wonderfully in similar circumstances before. } } } } Warren Straszheim } } } } } } ==============================Original Headers============================== } } 5, 29 -- From wesaia-at-iastate.edu Tue Nov 29 12:27:33 2011 } } 5, 29 -- Received: from mailexch-1.iastate.edu } } (mailexch-1.iastate.edu [129.186.140.21]) } } 5, 29 -- by ns.microscopy.com } } (8.12.11.20060308/8.12.8) with ESMTP id } } pATIRXFt021194 } } 5, 29 -- for {Microscopy-at-microscopy.com} ; Tue, 29 Nov 2011 12:27:33 -0600 } } 5, 29 -- Received: from } } EXITS702.ITS.IASTATE.EDU } } (exits702a.its.iastate.edu [10.10.87.140]) } } 5, 29 -- by mailexch-1.iastate.edu } } (8.14.4/8.12.10) with ESMTP id pATIRWF5016653; } } 5, 29 -- Tue, 29 Nov 2011 12:27:32 -0600 } } 5, 29 -- Received: from EXITS711.its.iastate.edu ([10.10.87.144]) by } } 5, 29 -- EXITS702A.its.iastate.edu } } ([10.10.87.140]) with mapi; Tue, 29 Nov 2011 } } 5, 29 -- 12:27:32 -0600 } } 5, 29 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} } } 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } } 5, 29 -- Date: Tue, 29 Nov 2011 12:27:32 -0600 } } 5, 29 -- Subject: Need PCD assembly or bellows for Auger probe } } 5, 29 -- Thread-Topic: Need PCD assembly or bellows for Auger probe } } 5, 29 -- Thread-Index: Acyuv5+5QXpeVoCqTZyXaW7BWHCZQA== } } 5, 29 -- } } Message-ID: {AA96B6F163D82F479AE1E4CA9000980F2905E86A29-at-EXITS711.its.iastate.edu} } } 5, 29 -- Accept-Language: en-US } } 5, 29 -- Content-Language: en-US } } 5, 29 -- X-MS-Has-Attach: } } 5, 29 -- X-MS-TNEF-Correlator: } } 5, 29 -- acceptlanguage: en-US } } 5, 29 -- Content-Type: text/plain; charset="us-ascii" } } 5, 29 -- MIME-Version: 1.0 } } 5, 29 -- X-PMX-Version: 5.6.1.2065439, } } Antispam-Engine: 2.7.2.376379, Antispam-Data: } } 2011.11.29.181814 } } 5, 29 -- X-ISUMailhub-test: Gauge=XIIIIIIII, Probability=18%, Report=' } } 5, 29 -- RATWARE_RCVD_BONUS_SPC 2.0, } } BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS } } 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS } } 0, BODY_SIZE_500_599 0, BODY_SIZE_7000_LESS 0, } } DATE_TZ_NA 0, FROM_NAME_PHRASE 0, NO_URI_FOUND } } 0, __CT 0, __CTE 0, __CT_TEXT_PLAIN 0, } } __HAS_MSGID 0, __MIME_TEXT_ONLY 0, } } __MIME_VERSION 0, __SANE_MSGID 0, } } __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, } } __TO_NO_NAME 0' } } 5, 29 -- Content-Transfer-Encoding: 8bit } } 5, 29 -- X-MIME-Autoconverted: from } } quoted-printable to 8bit by ns.microscopy.com } } id pATIRXFt021194 } } ==============================End of - Headers============================== } } ==============================Original Headers============================== } 9, 29 -- From jacques.faerber-at-ipcms.unistra.fr Wed Nov 30 02:40:52 2011 } 9, 29 -- Received: from mailhost.u-strasbg.fr } (mailhost.u-strasbg.fr [130.79.200.153]) } 9, 29 -- by ns.microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id } pAU8ep8b014404 } 9, 29 -- for {microscopy-at-microscopy.com} ; } Wed, 30 Nov 2011 02:40:52 -0600 } 9, 29 -- Received: from ipcms.u-strasbg.fr (ipcms.u-strasbg.fr [130.79.210.2]) } 9, 29 -- by mailhost.u-strasbg.fr } (8.14.3/jtpda-5.5pre1) with ESMTP id } pAU8eoQ4028559 } 9, 29 -- for } {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 } 09:40:50 +0100 (CET) (envelope-from } jacques.faerber-at-ipcms.unistra.fr) } 9, 29 -- Received: from [130.79.152.3] (odhinn.u-strasbg.fr [130.79.152.3]) } 9, 29 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) } 9, 29 -- (No client certificate requested) } 9, 29 -- by ipcms.u-strasbg.fr (Postfix) with ESMTP id BDE484A005 } 9, 29 -- for {microscopy-at-microscopy.com} ; } Wed, 30 Nov 2011 10:15:52 +0100 (CET) } 9, 29 -- Message-ID: {4ED5EC1C.4050303-at-ipcms.u-strasbg.fr} } 9, 29 -- Date: Wed, 30 Nov 2011 09:41:00 +0100 } 9, 29 -- From: "j.faerber" {jacques.faerber-at-ipcms.u-strasbg.fr} } 9, 29 -- User-Agent: Mozilla/5.0 (X11; U; Linux } i686; en-US; rv:1.9.2.23) Gecko/20110921 } Thunderbird/3.1.15 } 9, 29 -- MIME-Version: 1.0 } 9, 29 -- To: microscopy-at-microscopy.com } 9, 29 -- Subject: Re: [Microscopy] Need PCD } assembly or bellows for Auger probe } 9, 29 -- References: {201111291840.pATIeTq4005535-at-ns.microscopy.com} } 9, 29 -- In-Reply-To: {201111291840.pATIeTq4005535-at-ns.microscopy.com} } 9, 29 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 9, 29 -- Content-Transfer-Encoding: 8bit } 9, 29 -- X-IPCMS-MailScanner: Found to be clean } 9, 29 -- X-IPCMS-MailScanner-From: jacques.faerber-at-ipcms.unistra.fr } 9, 29 -- X-Greylist: Sender IP whitelisted, not } delayed by milter-greylist-4.2.5 } (mailhost.u-strasbg.fr [130.79.200.153]); Wed, } 30 Nov 2011 09:40:50 +0100 (CET) } 9, 29 -- X-Spam-Status: No, score=-100.0 required=5.0 tests=TO_NO_BRKTS_PCNT, } 9, 29 -- T_FRT_BELOW2,USER_IN_WHITELIST autolearn=disabled version=3.3.1 } 9, 29 -- X-Spam-Checker-Version: SpamAssassin } 3.3.1 (2010-03-16) on mr3.u-strasbg.fr } ==============================End of - Headers==============================
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 28 -- From oshel1pe-at-cmich.edu Wed Nov 30 07:07:54 2011 5, 28 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAUD7ssP004977 5, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 07:07:54 -0600 5, 28 -- Received: from cas1.central.cmich.local (sync.cmich.edu [141.209.15.75]) 5, 28 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pAUClpup013230 5, 28 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 07:47:51 -0500 5, 28 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 28 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Wed, 30 Nov 5, 28 -- 2011 08:07:52 -0500 5, 28 -- MIME-Version: 1.0 5, 28 -- Message-ID: {a06240802cafbdae989b1-at-[141.209.160.249]} 5, 28 -- In-Reply-To: {201111300844.pAU8iMuZ019423-at-ns.microscopy.com} 5, 28 -- References: {201111300844.pAU8iMuZ019423-at-ns.microscopy.com} 5, 28 -- Date: Wed, 30 Nov 2011 08:07:50 -0500 5, 28 -- To: {Microscopy-at-microscopy.com} 5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 28 -- Subject: [Microscopy] Re: Need PCD assembly or bellows for Auger probe 5, 28 -- Content-Type: text/plain; charset="iso-8859-1"; format=flowed 5, 28 -- X-Originating-IP: [141.209.160.249] 5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 28 -- X-Spam-Score: 0.70 () [Hold at 6.00] L_USD,L_USDOC,MIME_QP_LONG_LINE,_L_LLEXCH,Bayes(0.0001:-0.5) 5, 28 -- X-CanIt-Geo: ip=141.209.15.75; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 28 -- X-CanItPRO-Stream: default 5, 28 -- X-Canit-Stats-ID: 03G3oLPuf - b8cb8836b6f7 - 20111130 5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 5, 28 -- Content-Transfer-Encoding: 8bit 5, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pAUD7ssP004977 ==============================End of - Headers==============================
We are looking for a used single tilt cryoholder for our Tecnai T12. Needs to be compatible with FEI compustage.
Please contact me by email, if you have one to offer and we can discuss.
Regards Jon
Jon J McCarthy, Ph.D. Director, Shared Instrument Facilities Co-Director Advance Materials Industrial Consortium UW College of Engineering Room 272, Materials Science and Engineering 1509 University Ave, Madison WI 53706 Phone: 608-263-1073 Cell:608-345-6134 jjmccarthy-at-wisc.edu
==============================Original Headers============================== 6, 30 -- From jjmccarthy-at-wisc.edu Wed Nov 30 08:57:06 2011 6, 30 -- Received: from adsum.doit.wisc.edu (adsum.doit.wisc.edu [144.92.197.210]) 6, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pAUEv6Oe024252 6, 30 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 08:57:06 -0600 6, 30 -- MIME-version: 1.0 6, 30 -- Content-transfer-encoding: 7BIT 6, 30 -- Content-type: text/plain; CHARSET=US-ASCII 6, 30 -- Received: from avs-daemon.smtpauth1.wiscmail.wisc.edu by 6, 30 -- smtpauth1.wiscmail.wisc.edu 6, 30 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 6, 30 -- id {0LVH00802AV1N400-at-smtpauth1.wiscmail.wisc.edu} for 6, 30 -- Microscopy-at-microscopy.com; Wed, 30 Nov 2011 08:57:01 -0600 (CST) 6, 30 -- Received: from Proton (jjmccarthy.engr.wisc.edu [128.104.200.171]) 6, 30 -- by smtpauth1.wiscmail.wisc.edu 6, 30 -- (Sun Java(tm) System Messaging Server 7u2-7.05 32bit (built Jul 30 2009)) 6, 30 -- with ESMTPSA id {0LVH001MKAV00P10-at-smtpauth1.wiscmail.wisc.edu} for 6, 30 -- Microscopy-at-microscopy.com; Wed, 30 Nov 2011 08:57:00 -0600 (CST) 6, 30 -- Date: Wed, 30 Nov 2011 08:56:59 -0600 6, 30 -- From: Jon McCarthy {jjmccarthy-at-wisc.edu} 6, 30 -- Subject: TEM: Tecnai T12 Cryo Holder 6, 30 -- To: Microscopy-at-microscopy.com 6, 30 -- Message-id: {003f01ccaf70$4fe22380$efa66a80$-at-wisc.edu} 6, 30 -- Organization: Uw-Madison 6, 30 -- X-Mailer: Microsoft Outlook 14.0 6, 30 -- Content-language: en-us 6, 30 -- Thread-index: AcyvcEirujeiAoKbTvGD7KR3zDBRBg== 6, 30 -- X-Spam-Report: AuthenticatedSender=yes, SenderIP=128.104.200.171 6, 30 -- X-Spam-PmxInfo: Server=avs-14, Version=5.6.1.2065439, 6, 30 -- Antispam-Engine: 2.7.2.376379, Antispam-Data: 2011.11.30.144514, 6, 30 -- SenderIP=128.104.200.171 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eoptics-at-mcmaster.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eoptics-at-mcmaster.ca
Name: Fred Pearson
Organization: McMaster University
Title-Subject: [Filtered] TEM JEOL Double Tilt Hot Stage Controller
Message: I am looking for circuit diagrams or manual for a Gatan older model Beta controller for the Y tilt for our hotstage.
The model number of this unit is 646-0300.
The cables and holder check as being okay, but the controller will not advance the tilt.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Georgianne.Ciraolo-at-cchmc.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Cincinnati Chhildren's Hospital Medical Center
Title-Subject: [Filtered] TEM of fetal mice eyes
Message: I have recently been approached to study the eyes of fetal mice. My standard fixation would be 3% Glut/ cacodylate buffer but I am wonder if anyone has a better fixative and would to share.
Thanks in advance for your help.
Georgianne Ciraolo em tech II Georgianne.Ciraolo-at-cchmc.org
what book would you suggest as a good introduction to the theory of Atomic Force Microscopy for protein / biology researchers please?
Thank you beforehand.
With kind regards, Dmitry
-- Dr. Dmitry Sokolov Honorary Research Associate Institute of Fundamental Sciences Riddet Road, Massey University Private Bag 11-222, Palmerston North Mob: +64(21)063-5382
==============================Original Headers============================== 7, 18 -- From d.sokolov-at-massey.ac.nz Wed Nov 30 19:08:05 2011 7, 18 -- Received: from mu-mail3.massey.ac.nz (mu-mail3.massey.ac.nz [130.123.129.187]) 7, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB1184IR021989 7, 18 -- for {Microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 19:08:05 -0600 7, 18 -- Received: from TUR-MM3.massey.ac.nz (tur-mm3.massey.ac.nz [130.123.128.140]) 7, 18 -- by mu-mail3.massey.ac.nz (Postfix) with ESMTP id 4CA3C1B30F2 7, 18 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 14:08:03 +1300 (NZDT) 7, 18 -- Received: from [127.0.0.1] (Not Verified[10.100.189.3]) by TUR-MM3.massey.ac.nz with MailMarshal 7, 18 -- id {B4ed6d3730000} ; Thu, 01 Dec 2011 14:08:03 +1300 7, 18 -- Message-ID: {4ED6D370.2030108-at-massey.ac.nz} 7, 18 -- Date: Thu, 01 Dec 2011 14:08:00 +1300 7, 18 -- From: Dmitry Sokolov {d.sokolov-at-massey.ac.nz} 7, 18 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:6.0.1) Gecko/20110830 Thunderbird/6.0.1 7, 18 -- MIME-Version: 1.0 7, 18 -- To: Microscopy-at-microscopy.com 7, 18 -- Subject: Theory of AFM for beginners 7, 18 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 18 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I study the ultrastructure of tadpole eyes. Your standard fix should work but you should section the cornea to ensure better penetration of the fixative. I usually enucleate the eyes, put them in cold fix for 1/2 -1h (tadpole eyes are approximately 1mm3), puncture the cornea, and return them to cold fix. You should work the dehydration/ embedding times closely as they tend to vary with species. For the tadpole eyes, for instance, I usually do 1h dehydration steps at RT.
Good luck!
Tami
================================
Tami Bogea, PhD Research Associate University of British Columbia Department of Ophthalmology & Visual Sciences 2250 Willow St. Vancouver BC V5Z 3N9 P: (604) 875-4648
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both Georgianne.Ciraolo-at-cchmc.org as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Organization: Cincinnati Chhildren's Hospital Medical Center
Title-Subject: [Filtered] TEM of fetal mice eyes
Message: I have recently been approached to study the eyes of fetal mice. My standard fixation would be 3% Glut/ cacodylate buffer but I am wonder if anyone has a better fixative and would to share.
Thanks in advance for your help.
Georgianne Ciraolo em tech II Georgianne.Ciraolo-at-cchmc.org
I don't know about fetal eyes, but adult retinas tend to detach after just glut fixation. Check the literature & Paul Webster at the Eye Institute will probably have the best input.
Roger Moretz
On Wed, Nov 30, 2011 at 7:58 PM, { microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both } Georgianne.Ciraolo-at-cchmc.org as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: Georgianne.Ciraolo-at-cchmc.org Name: Georgianne Ciraolo } } Organization: Cincinnati Chhildren's Hospital Medical Center } } Title-Subject: [Filtered] TEM of fetal mice eyes } } Message: I have recently been approached to study the eyes of fetal mice. } My standard fixation } would be 3% Glut/ cacodylate buffer but I am wonder if anyone has a better } fixative and would to share. } } Thanks in advance for your help. } } Georgianne Ciraolo } em tech II } Georgianne.Ciraolo-at-cchmc.org } } } Login Host: 205.142.197.75 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== } 11, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Nov 30 } 18:53:16 2011 } 11, 25 -- Received: from znl.com ([206.69.208.20]) } 11, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id pB10rGbH021888 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 } 18:53:16 -0600 } 11, 25 -- Received: from localhost (localhost [127.0.0.1]) } 11, 25 -- by znl.com (Postfix) with ESMTP id F29E344CDF9 } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 } 18:53:15 -0600 (CST) } 11, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 11, 25 -- Received: from znl.com ([127.0.0.1]) } 11, 25 -- by localhost (server.microscopy.com [127.0.0.1]) } (amavisd-new, port 10024) } 11, 25 -- with ESMTP id sEkXduogqz8p for {microscopy-at-microscopy.com } } ; } 11, 25 -- Wed, 30 Nov 2011 18:53:15 -0600 (CST) } 11, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com } [206.69.208.22]) } 11, 25 -- by znl.com (Postfix) with ESMTPA id 8B20344CDEE } 11, 25 -- for {microscopy-at-microscopy.com} ; Wed, 30 Nov 2011 } 18:53:15 -0600 (CST) } 11, 25 -- Message-ID: {4ED6CFFB.2020501-at-microscopy.com} } 11, 25 -- Date: Wed, 30 Nov 2011 18:53:15 -0600 } 11, 25 -- From: MicroscopyListserver-NoReply { } microscopylistserver-noreply-at-microscopy.com} } 11, 25 -- Reply-To: Georgianne.Ciraolo-at-cchmc.org } 11, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 11, 25 -- MIME-Version: 1.0 } 11, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 25 -- Subject: [Filtered] viaWWW:TEM of fetal mice eyes } 11, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 11, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers============================== }
==============================Original Headers============================== 5, 27 -- From microscopylistserver-noreply-at-microscopy.com Thu Dec 1 07:44:36 2011 5, 27 -- Received: from znl.com ([206.69.208.20]) 5, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB1DiYZt023006 5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 07:44:35 -0600 5, 27 -- Received: from localhost (localhost [127.0.0.1]) 5, 27 -- by znl.com (Postfix) with ESMTP id 483B644D4F9 5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 07:44:34 -0600 (CST) 5, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 5, 27 -- Received: from znl.com ([127.0.0.1]) 5, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 5, 27 -- with ESMTP id KcAHUhzP5A8S for {microscopy-at-microscopy.com} ; 5, 27 -- Thu, 1 Dec 2011 07:44:33 -0600 (CST) 5, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 5, 27 -- by znl.com (Postfix) with ESMTPA id E2EFD44D4EE 5, 27 -- for {microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 07:44:33 -0600 (CST) 5, 27 -- Message-ID: {4ED784C1.7090209-at-microscopy.com} 5, 27 -- Date: Thu, 01 Dec 2011 07:44:33 -0600 5, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 5, 27 -- Reply-To: Roger Moretz {rcmoretz-at-gmail.com} 5, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 27 -- MIME-Version: 1.0 5, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 5, 27 -- Subject: [Filtered] Re: [Microscopy] viaWWW:TEM of fetal mice eyes 5, 27 -- References: {201112010058.pB10wPI3000419-at-ns.microscopy.com} 5, 27 -- In-Reply-To: {201112010058.pB10wPI3000419-at-ns.microscopy.com} 5, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 27 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both monica.nelea-at-polymtl.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] immunogold labelling of chitosan for TEM
Message: Hello everyone, I would like to ask if someone of you have experience in immunogold labelling of chitosan. If yes, could you please let me know about how to proceed: steps, schemas, protocols to be applied? Any advice is highly welcomed ant much appreciated. Thanks in advance. Monica
Please excuse this rude interruption of your important work.
The EM program at Delta College is calling out for attention. We made a Facebook page at
Electron Microscopy at SJ Delta College
and we invite everyone to take a look. We want to reach out to everyone to help us maintain and improve our program. So, if you are a past, present, or future student, a potential sponsor or employer, or just want to know more about us, stop by and take a look.
Thanks
Jon
Jonathan Krupp Delta College 5151 Pacific Ave. Box 212 Stockton, CA 95207 209-954-5284 jkrupp-at-deltacollege.edu
Find us on Facebook -at- Electron Microscopy at SJ Delta College
==============================Original Headers============================== 16, 34 -- From jkrupp-at-deltacollege.edu Thu Dec 1 16:21:27 2011 16, 34 -- Received: from mailin.deltacollege.edu (mailin.deltacollege.edu [207.62.178.150]) 16, 34 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pB1MLRXo018133 16, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 16:21:27 -0600 16, 34 -- Received: from mailin.deltacollege.edu (localhost.localdomain [127.0.0.1]) 16, 34 -- by localhost (Email Security Appliance) with SMTP id E268C966C2C_ED7FDE6B 16, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 22:21:26 +0000 (GMT) 16, 34 -- Received: from sunspot.sjdccd.cc.ca.us (mail2.sjdccd.cc.ca.us [207.62.178.20]) 16, 34 -- by mailin.deltacollege.edu (Sophos Email Appliance) with ESMTP id C7FB0966B92_ED7FDE6F 16, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 22:21:26 +0000 (GMT) 16, 34 -- Received: from zmail.deltacollege.edu ([207.62.178.179]) by 16, 34 -- sunspot.sjdccd.cc.ca.us (Netscape Messaging Server 4.15) with 16, 34 -- ESMTP id LVJQ3Q00.H7L for {Microscopy-at-microscopy.com} ; Thu, 1 16, 34 -- Dec 2011 14:21:26 -0800 16, 34 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 16, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTP id 987E611194935 16, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 14:21:26 -0800 (PST) 16, 34 -- Received: from zmail.deltacollege.edu ([127.0.0.1]) 16, 34 -- by localhost (zmail.deltacollege.edu [127.0.0.1]) (amavisd-new, port 10024) 16, 34 -- with ESMTP id 8bUMZ+Bf5vJe for {Microscopy-at-microscopy.com} ; 16, 34 -- Thu, 1 Dec 2011 14:21:26 -0800 (PST) 16, 34 -- Received: from [172.20.2.133] (unknown [172.20.2.133]) 16, 34 -- by zmail.deltacollege.edu (Postfix) with ESMTPSA id 517CF11194903 16, 34 -- for {Microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 14:21:26 -0800 (PST) 16, 34 -- From: Jon Krupp {jkrupp-at-deltacollege.edu} 16, 34 -- Content-Type: text/plain; charset=us-ascii 16, 34 -- Subject: Blatant call for attention 16, 34 -- Date: Thu, 1 Dec 2011 14:21:25 -0800 16, 34 -- Message-Id: {3D068303-8E56-45D0-B4EC-BABEF2E8D756-at-deltacollege.edu} 16, 34 -- To: Microscopy-at-microscopy.com 16, 34 -- Mime-Version: 1.0 (Apple Message framework v1084) 16, 34 -- X-Mailer: Apple Mail (2.1084) 16, 34 -- Content-Transfer-Encoding: 8bit 16, 34 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB1MLRXo018133 ==============================End of - Headers==============================
There are many protocols for immuno labelling which may give reasonable results with many antigens. If you need one, you could start by checking out the website of gold reagent suppliers or delve into the wealth of info archived from messages that were sent in to this List over the past years .
Labelling of chitosan may be a bit more tricky than labelling the average antigen (if there is such a thing). With the abundance of repeating amino groups in the chitosan backbone there is a fair chance of false positive background. If this should happen it is likely because of a polar interaction between NH3+ groups and negatively charged gold particles.
This type of interaction can be suppressed by using one or a combination of the following:
-- incubate at a slightly basic pH, perhaps about 8.4 using e.g. Tris buffered saline. The number of protonated groups will be lowered with higher pH. -- use a relatively high NaCl concentration in the incubation buffer. This will suppress charge interactions similar to what happens in ion exchange chromatography. For some of the antigens that I have played around with NaCl concentrations between 300 and 600 mM gave excellent results with both reduced background and increased specific results. When working with hydrated tissue or sections you may want to be cautious, however, because the structural preservation may become affected. -- use a poly-anionic additive to the incubation buffer. Poly-anions have the same sign charge as the gold particle, at least at a pH of 7 or higher . They will therefor compete with gold particles for binding to NH3+ groups, thus reducing the background by competition.
Having said that: of course always start out applying a standard protocol. If that works then all the theory and worries were unnecessary.
Please feel free to contact me off list if you need more info.
Bonne chance!
Jan Leunissen
Aurion ImmunoGold Reagents
http://www.aurion.nl
On 2/12/2011, at 10:56 AM, microscopylistserver-noreply-at-microscopy.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } monica.nelea-at-polymtl.ca as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: monica.nelea-at-polymtl.ca Name: Monica Nelea } } Organization: Ecole Polytechnique } } Title-Subject: [Filtered] immunogold labelling of chitosan for TEM } } Message: Hello everyone, } I would like to ask if someone of you have experience in immunogold labelling of chitosan. If yes, } could you please let me know about how to proceed: steps, schemas, protocols to be applied? Any } advice is highly welcomed ant much appreciated. } Thanks in advance. } Monica } } } Login Host: 132.207.56.49 } ---------------------------------------------------------------------------
==============================Original Headers============================== 18, 21 -- From leunissen-at-aurion.nl Thu Dec 1 17:38:06 2011 18, 21 -- Received: from fep01.xtra.co.nz (fep01.xtra.co.nz [210.54.141.245]) 18, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB1Nc4of003941 18, 21 -- for {microscopy-at-microscopy.com} ; Thu, 1 Dec 2011 17:38:05 -0600 18, 21 -- Received: from unknown-d8-a2-5e-8d-da-93.lan ([122.57.253.89]) 18, 21 -- by fep01.xtra.co.nz with ESMTP 18, 21 -- id {20111201233758.ZSAZ5701.fep01.xtra.co.nz-at-unknown-d8-a2-5e-8d-da-93.lan} ; 18, 21 -- Fri, 2 Dec 2011 12:37:58 +1300 18, 21 -- Subject: Re: [Microscopy] [Filtered] MicroscopyListserverviaWWW:immunogold labelling of chitosan 18, 21 -- Mime-Version: 1.0 (Apple Message framework v1084) 18, 21 -- Content-Type: text/plain; charset=us-ascii 18, 21 -- From: Jan Leunissen - Aurion {leunissen-at-aurion.nl} 18, 21 -- In-Reply-To: {201112012156.pB1Luaws001242-at-ns.microscopy.com} 18, 21 -- Date: Fri, 2 Dec 2011 12:37:56 +1300 18, 21 -- Cc: microscopy-at-microscopy.com 18, 21 -- Message-Id: {8411336D-C143-4AEF-9AC1-FA7CF167A16E-at-aurion.nl} 18, 21 -- References: {201112012156.pB1Luaws001242-at-ns.microscopy.com} 18, 21 -- To: monica.nelea-at-polymtl.ca 18, 21 -- X-Mailer: Apple Mail (2.1084) 18, 21 -- Content-Transfer-Encoding: 8bit 18, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB1Nc4of003941 ==============================End of - Headers==============================
HI Monica, Below is our basic procedure for gold-labeling. Like Jan said the NaCl concentration matters. We tend to use a high salt content in the buffer. good luck with your labeling, Beth
GOLD LABELING PROTOCOL Sections must be on gold or gold-gilded grids.
Use a petri dish to create a moist chamber - wet filter paper with H2O (to keep moisture level high) then put a piece of parafilm on top of the moist filter paper. Do controls in a separate petri dish.
1. Place grid on a drop of KPBS (potassium phosphate buffered saline, 0.1M KPB and 0.5M NaCl, pH 7.2) - hydrate 5-10 minutes.
2. Next drop - 3% skim milk ((0.03g non-fat milk in 1 ml buffer), for 1 hour. Prepared fresh and centrifuged for a few seconds before use.
3. Next drop - KPBS - 5 minutes.
4. 1° Ab - dilution of your choice (usually 1:20) - 1 hour or more (centrifuge briefly before use). For a control = OMIT the primary antibody.
5. Rinse in buffer (use squeeze bottle), remove excess H2O with a filter paper.
6. 2° Ab - dilution of your choice (usually 1:50), 1 hr (centrifuge briefly before use).
7. Rinse for 30 sec in buffer (use squeeze bottle).
8. Rinse for 30 sec in dH2O (use squeeze bottle).
9. Post stain with uranyl acetate and lead citrate.
Variation: Use less salt in the buffer or different blocking agent or longer incubation times or different dilutions of primary and/or secondary antibodies.
Make Stock Buffer:
A) Potassium phosphate mono (KH2PO4) 1M – 6.8 g/50 ml ddH2O.
B) Potassium phosphate dibasic (K2HPO4) 1M – 8.71g/50 ml ddH2O
Use 1.6 ml of A + 8.4 ml of B = 10 ml Then add 90 ml dd H2O = 100 ml of stock buffer.
Put 14.61 g of NaCl in 450 ml of dd H2O* and stir. Then add 50 ml of stock buffer = 500 ml of 0.1 M KPBS in 0.5 M NaCl and stir.
==============================Original Headers============================== 19, 21 -- From beth-at-plantbio.uga.edu Fri Dec 2 08:52:04 2011 19, 21 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 19, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB2Eq4t9006698 19, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2011 08:52:04 -0600 19, 21 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 19, 21 -- Received: from [128.192.26.69] ([128.192.26.69]) 19, 21 -- (authenticated user beth-at-plantbio.uga.edu) 19, 21 -- by dogwood.plantbio.uga.edu 19, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 19, 21 -- for microscopy-at-microscopy.com; 19, 21 -- Fri, 2 Dec 2011 09:52:04 -0500 19, 21 -- Message-Id: {864C0DAC-06C5-4F88-9F03-ED2DE75E47B8-at-plantbio.uga.edu} 19, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 19, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com} 19, 21 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 19, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 19, 21 -- Subject: immunogold labeling of chitosan 19, 21 -- Date: Fri, 2 Dec 2011 09:52:03 -0500 19, 21 -- X-Mailer: Apple Mail (2.936) 19, 21 -- Content-Transfer-Encoding: 8bit 19, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB2Eq4t9006698 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bplowman as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bplowman Name: Barbara Plowman
Organization: University of the Pacific
Title-Subject: [Filtered] HIV or related
Message: Excuse me. I thought this was an open forum! I underst5and negative staining HIV is not the same as HIV cell infected research. But, the issue was of safety and our group has a P3 level laboratory. Safety precautions are of concern when working with any pathogenic organism. I was not treating it lightly.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
I sometimes use a similar mold from Pella. I find that getting the ACLAR onto the thing without trapping bubbles to be annoying, especially with the shrinkage of LRW during polymerization. Instead, I just overfill the cavities slightly, orient the specimens as desired, and put the filled mold into a plastic dish with sides about 1 cm high (so the bag won't touch the resin) and I put this whole thing into a zip lock bag and fill it with nitrogen (or argon from the sputter coater tank). Just zip the bag mostly closed over the gas tubing, get a stream of gas flowing and let the bag fill, pushing down on the bag a couple of times to purge O2, then let it fill a bit, pull out the tubing, and press the final cm of zip lock closed. Then load it into the oven at ~58 deg C for 2-2.5 hrs. If you put enough resin into the mold that the cavities are joined, they will actually come out as a single strip that is pretty convenient, as getting the blocks out of the mold can be a little tricky because it's not as flexible as the silicone molds.
Hope this helps,
Andy Bowling
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, December 02, 2011 11:06 AM To: Bowling, Andrew (AJ)
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
In our experience it is better to not fill all the molds with samples. We have to orientate drosophila retina and larvae very strategically. We place our samples into the center 6 wells, overfill the wells including two extra empty ones on either side of the samples. Press the Aclar film over the samples first then gently lay out the film toward both sides so that the resin pours over into the left-over empty well spaces. The very end well spaces will not polymerise very well, but the ones with the samples will turn out very well, at least for us. We then place the mold into a 60 degree oven overnight to two days. So far we have been able to thick section and do fluorescence staining with no problem. Hope this helps.
Lita Duraine EM Technologist Howard Hughes Medical Institute Molecular Genetics Duncan Neurological Research Institute 1250 Moursand St. Houston, TX 77030 room: N1165.17 mailstop: NR1125 832-824-8772 EM Lab 832-824-8750 Main Lab 979-549-6526 Cell
-----Original Message----- X-from: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Friday, December 02, 2011 10:08 AM To: Duraine, Lita
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tbogea-at-mail.ubc.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea.suarez-at-ucdenver.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: andrea.suarez-at-ucdenver.edu Name: Andrea
Organization: CU dermpath
Title-Subject: [Filtered] mounting on carbon planchets
Message: We are doing a project where we will be doing QEMSCAN, which has the capacity to do a very sophisticated analysis of mineral composition. We will be looking for volcanic ash deposition in human skin samples, and would like to embedd the tissues in as pure a fashion as possible, with optimum antigen preservation. We have some concerns about parrafin embedding, as we plan to mount the sections onto carbon planchets. Our experience w/ this in the past, is that adherence to the planchet is poor after deparafinizaiton. Therefore, we would like to optimize adherence, if possible, or avoid the need for deparafinization by embedding in a wax (such as carnuba) w/ a very high melting temp.
1.) Does anyone have any experience/insight regarding embedding in carnuba wax? 2.) Any recomendations as to how to improve adherence to the carbon planchet in the event that we do end up having to de-parafinize? Instead of paraffin, PEG embedding may be better, and was reading about using agarose blocks to improve transfer to slides. Does anyone have any experience/recommendations regarding agarose block method? We were thinking that adding albumin to the water bath may help things too. Other thoughts include coating the planchets w/ a carbon paste.
Many thanks and I look forward to hearing from you.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both maryard-at-uga.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: maryard-at-uga.edu Name: Mary Ard
Organization: College of Vet Med, University of Georgia
Message: Responding to Dr. Tami Bogea: Dr. Bogea, we use the PTFE flat mold all the time for LRWhite resin. We consistently use the protocol suggested in the Technical Data Sheet that accompanies the product. We have always gotten excellent results.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both hchen3-at-unl.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: hchen3-at-unl.edu Name: Han Chen
Organization: University of Nebraska Lincoln
Title-Subject: [Filtered] TEM HIV negative staining one more biosafe question
Message: I would like to say thank you for all the people who replied my post. My user will bring fixed HIV samples(fixed by 2% glutaraldehyde for 2 hours in room time). So the user will do negative staining for the fixed HIV samples. My next question will be: Dose the fixed HIV samples will be OK for BSL1 lab? Any suggestion will be welcome. Thanks.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both bob.price-at-uscmed.sc.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: bob.price-at-uscmed.sc.edu Name: Bob Price
Organization: Univ South Carolina
Title-Subject: [Filtered] Microscopy and Microanalysis
Message: Dear All,
As you try to get that last manuscript submission of 2011 off of your list of things to do please consider sending your work to Microscopy and Microanalysis, the Journal of the Microscopy Society of America. MAM consistently has one of the highest impact factors among imaging related journals (currently 3.25) and with our recently approved contract with Cambridge University Press we now offer free full color printing throughout the journal. Other advantages of publishing in MAM include:
No submission charges Electronic submission through Manuscript Central (http://mc.manuscriptcentral.com/mam) Online publication ahead of print Online publication of supplemental data including short videos and other multimedia files Indexed in Pubmed and Medlines Cambridge Journals alert systems to insure the research community is alerted to publication of your work Descriptive technique development, review and original research articles are published. For more information visit the Microscopy Society of America ( http://www.microscopy.org ) or Cambridge Journals Online ( http://journals.cambridge.org/mam ) websites
Bob
Bob Price Research Professor Editor-in-Chief, Microscopy and Microanalysis USC School of Medicine 6439 Garner's Ferry Road Columbia, SC 29209 803-216-3824 (T) (803) 216-3847 (F) 803-216-3825 (Admin Asst) Bob.Price-at-uscmed.sc.edu
In preparing lung biopsies with asbestosis for SEM/EDX, I took parafin embedded tissue sections and mounted them on carbon double stick tape on aluminum stubs, then rinsed them with xylene to remove the parafin, did a quick 100% ethanol drying and carbon coated the samples. I was able to both see good structure and identify the ferruginous bodies by secondary and backscattered imaging and EDS without a problem with this technique.
Ed Haller
Edward Haller, Lab Manager University of South Florida Integrative Biology Department Electron Microscopy Core SCA 110 4202 East Fowler Avenue Tampa, FL 33620 813-974-2676 ehaller-at-usf.edu Office: LSA 119 ________________________________________ X-from: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Friday, December 02, 2011 6:42 PM To: Haller, Edward
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both andrea.suarez-at-ucdenver.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: andrea.suarez-at-ucdenver.edu Name: Andrea
Organization: CU dermpath
Title-Subject: [Filtered] mounting on carbon planchets
Message: We are doing a project where we will be doing QEMSCAN, which has the capacity to do a very sophisticated analysis of mineral composition. We will be looking for volcanic ash deposition in human skin samples, and would like to embedd the tissues in as pure a fashion as possible, with optimum antigen preservation. We have some concerns about parrafin embedding, as we plan to mount the sections onto carbon planchets. Our experience w/ this in the past, is that adherence to the planchet is poor after deparafinizaiton. Therefore, we would like to optimize adherence, if possible, or avoid the need for deparafinization by embedding in a wax (such as carnuba) w/ a very high melting temp.
1.) Does anyone have any experience/insight regarding embedding in carnuba wax? 2.) Any recomendations as to how to improve adherence to the carbon planchet in the event that we do end up having to de-parafinize? Instead of paraffin, PEG embedding may be better, and was reading about using agarose blocks to improve transfer to slides. Does anyone have any experience/recommendations regarding agarose block method? We were thinking that adding albumin to the water bath may help things too. Other thoughts include coating the planchets w/ a carbon paste.
Many thanks and I look forward to hearing from you.
I'm posting this question for one of our researchers. Any suggestions?
I am doing some immunocytochemistry on cryosections and parraffin secions. The main problem I am having deals with proper fixation -- These insects are unique in that they are viviparous and give birth to fully developed larvae. I have been able to fix young females by injecting them with 4% paraformaldehyde followed by 3 days at 4C in fixative. After this I cut them in half with a razor and refix for several more days. The problem is when I look at blood fed or pregnant flies- Everything is getting fixed except the developing larvae and the blood filled alimentary tract. I have stayed with paraformaldehyde because I nne to immunostain sections. In addition when I section the paraffin embedded material the specimens tear if I attempt to cut sections smaller then seven microns. Do you have any recommendations of other fixatives that would be compatible with immunostaining???
Thank you Karen Kelley University of Florida
==============================Original Headers============================== 7, 39 -- From vau-at-ufl.edu Tue Dec 6 08:17:36 2011 7, 39 -- Received: from smtp.ufl.edu (smtp02.osg.ufl.edu [128.227.74.165]) 7, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB6EHZqb020417 7, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Dec 2011 08:17:36 -0600 7, 39 -- Received: from ufexch-hub01.ad.ufl.edu (ufexch-hub01.ad.ufl.edu [10.241.69.102]) 7, 39 -- by smtp.ufl.edu (8.14.0/8.14.0/3.0.0) with ESMTP id pB6EHOLT028192 7, 39 -- (version=TLSv1/SSLv3 cipher=RC4-MD5 bits=128 verify=NOT) 7, 39 -- for {Microscopy-at-microscopy.com} ; Tue, 6 Dec 2011 09:17:31 -0500 7, 39 -- Received: from UFEXCH-MBXN01.ad.ufl.edu (10.19.251.71) by 7, 39 -- ufexch-hub01.ad.ufl.edu (10.241.69.102) with Microsoft SMTP Server (TLS) id 7, 39 -- 8.2.255.0; Tue, 6 Dec 2011 09:17:18 -0500 7, 39 -- Received: from UFEXCH-MBXN04.ad.ufl.edu ([fe80::e96d:2947:fdce:ccee]) by 7, 39 -- UFEXCH-MBXN01.ad.ufl.edu ([fe80::3119:c29a:e128:2d6f%18]) with mapi id 7, 39 -- 14.01.0339.001; Tue, 6 Dec 2011 09:17:18 -0500 7, 39 -- From: "Kelley,Karen L" {vau-at-ufl.edu} 7, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 39 -- Subject: insect paraffin embedding problem 7, 39 -- Thread-Topic: insect paraffin embedding problem 7, 39 -- Thread-Index: AQHMtCHCLY4QkxFU70WoWF3c3Pcbnw== 7, 39 -- Date: Tue, 6 Dec 2011 14:17:17 +0000 7, 39 -- Message-ID: {CB038E1C.168B1%vau-at-ufl.edu} 7, 39 -- Accept-Language: en-US 7, 39 -- Content-Language: en-US 7, 39 -- X-MS-Has-Attach: 7, 39 -- X-MS-TNEF-Correlator: 7, 39 -- user-agent: Microsoft-MacOutlook/14.12.0.110505 7, 39 -- x-originating-ip: [128.227.0.118] 7, 39 -- Content-Type: text/plain; charset="us-ascii" 7, 39 -- Content-ID: {BB37BB241A6C1A43A6BF790190DC1B2D-at-ad.ufl.edu} 7, 39 -- MIME-Version: 1.0 7, 39 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.5.7110,1.0.211,0.0.0000 7, 39 -- definitions=2011-12-06_04:2011-12-06,2011-12-06,1970-01-01 signatures=0 7, 39 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 ipscore=0 suspectscore=0 7, 39 -- phishscore=0 bulkscore=0 adultscore=0 classifier=spam adjust=0 reason=mlx 7, 39 -- engine=6.0.2-1012030000 definitions=main-1112060105 7, 39 -- X-Spam-Level: * 7, 39 -- X-UFL-Spam-Level: * 7, 39 -- Content-Transfer-Encoding: 8bit 7, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB6EHZqb020417 ==============================End of - Headers==============================
From johnnarzika-at-ymail.com Tue Dec 6 08:20:51 2011 Return-Path: {johnnarzika-at-ymail.com} Received: from mail.medianet.com.tw (61-219-149-7.HINET-IP.hinet.net [61.219.149.7]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB6EKoDJ021938; Tue, 6 Dec 2011 08:20:50 -0600 Received: from User (unknown [82.128.0.134]) by mail.medianet.com.tw (Postfix) with ESMTP id C0C093B0836; Tue, 6 Dec 2011 21:55:49 +0800 (CST) Reply-To: {collinsobi-at-yahoo.cn}
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both donk-at-ardl.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: donk-at-ardl.com Name: Don Kierstead
Organization: Akron Rubber Development Laboratories, Inc.
Title-Subject: [Filtered] RE: EDX Spectrum of Silicone Rubber
Message: I am having trouble with interpreting an EDX spectrum of a silicone rubber sample. The elements detected are carbon (8295 counts)), oxygen (13,483 counts), and silicon (90549). So, as you can see, I have a really big silicon peak with oxygen being a moderate sized peak and carbon small. The problem I am having is that I'm getting a small (910 cnt) peak at 2.235KeV that doesn't really line up with any specific x-rays on any of the charts and does not appear to be an escape or sum peak for any of the elements present. The closest identification would be either thallium or mercury, but it is not likely They would be present in the rubber. I do get a good sized silicon sum peak at 3.465KeV (1136 cnt.). The question to the list is whether or not this peak is an artifact or perhaps a silicon x-ray peak that is not listed on the charts. I am operating an IXRF EDS 2008 computer software. Any feedback would be greatly appreciated!
I've been asked to image Gallistan (Gallium, Tin, Indium) in our SEM. Note that the SEM is not a variable pressure system. Should I be concerned about outgassing and contamination of the specimen chamber?
Thanks
Owen Mills Michigan Tech University Houghton, MI
==============================Original Headers============================== 4, 20 -- From prvs=315ad1d10=opmills-at-mtu.edu Wed Dec 7 07:53:45 2011 4, 20 -- Received: from mail.mtu.edu (mx1.mtu.edu [141.219.73.15]) 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB7DrjGU023864 4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 07:53:45 -0600 4, 20 -- X-IronPort-Anti-Spam-Filtered: true 4, 20 -- X-IronPort-Anti-Spam-Result: AvsEAOhu306N20GI/2dsb2JhbABDqlOBBYIzgX2IHJZflkWJBYpRYwSILp5+ 4, 20 -- X-IronPort-AV: E=Sophos;i="4.71,313,1320642000"; 4, 20 -- d="scan'208";a="476848913" 4, 20 -- Received: from e12-0626-fac.coe.ad.mtu.edu ([141.219.65.136]) 4, 20 -- by email.mtu.edu with ESMTP/TLS/AES128-SHA; 07 Dec 2011 08:53:44 -0500 4, 20 -- From: "Owen P. Mills" {opmills-at-mtu.edu} 4, 20 -- Content-Type: text/plain; charset=us-ascii 4, 20 -- Subject: Materials: Gallistan in SEM 4, 20 -- Date: Wed, 7 Dec 2011 08:53:44 -0500 4, 20 -- Message-Id: {F61531C1-B0F0-4F77-8C91-726860D3A2B3-at-mtu.edu} 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) 4, 20 -- X-Mailer: Apple Mail (2.1084) 4, 20 -- Content-Transfer-Encoding: 8bit 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB7DrjGU023864 ==============================End of - Headers==============================
In - Indium is UHV compatible Ga - Gallium is vacuum-compatible at least till 10-9Torr Sn - Tin (as metal) should be UHV compatible
Alloy has low melting temperature, but I'd expect that vapor pressure at room temperature would still be much lower then 10-8Torr - good enough for high-vacuum SEM...
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 12/7/2011 8:54 AM, opmills-at-mtu.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Good morning. } } I've been asked to image Gallistan (Gallium, Tin, Indium) in our SEM. Note that the SEM is not a variable pressure system. Should I be concerned about outgassing and contamination of the specimen chamber? } } Thanks } } Owen Mills } Michigan Tech University } Houghton, MI } } ==============================Original Headers============================== } 4, 20 -- From prvs=315ad1d10=opmills-at-mtu.edu Wed Dec 7 07:53:45 2011 } 4, 20 -- Received: from mail.mtu.edu (mx1.mtu.edu [141.219.73.15]) } 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB7DrjGU023864 } 4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 07:53:45 -0600 } 4, 20 -- X-IronPort-Anti-Spam-Filtered: true } 4, 20 -- X-IronPort-Anti-Spam-Result: AvsEAOhu306N20GI/2dsb2JhbABDqlOBBYIzgX2IHJZflkWJBYpRYwSILp5+ } 4, 20 -- X-IronPort-AV: E=Sophos;i="4.71,313,1320642000"; } 4, 20 -- d="scan'208";a="476848913" } 4, 20 -- Received: from e12-0626-fac.coe.ad.mtu.edu ([141.219.65.136]) } 4, 20 -- by email.mtu.edu with ESMTP/TLS/AES128-SHA; 07 Dec 2011 08:53:44 -0500 } 4, 20 -- From: "Owen P. Mills" {opmills-at-mtu.edu} } 4, 20 -- Content-Type: text/plain; charset=us-ascii } 4, 20 -- Subject: Materials: Gallistan in SEM } 4, 20 -- Date: Wed, 7 Dec 2011 08:53:44 -0500 } 4, 20 -- Message-Id: {F61531C1-B0F0-4F77-8C91-726860D3A2B3-at-mtu.edu} } 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } 4, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) } 4, 20 -- X-Mailer: Apple Mail (2.1084) } 4, 20 -- Content-Transfer-Encoding: 8bit } 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB7DrjGU023864 } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 39 -- From vray-at-partbeamsystech.com Wed Dec 7 09:43:52 2011 4, 39 -- Received: from nm27.access.bullet.mail.mud.yahoo.com (nm27.access.bullet.mail.mud.yahoo.com [66.94.237.92]) 4, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pB7Fhp85010562 4, 39 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 09:43:52 -0600 4, 39 -- Received: from [66.94.237.199] by nm27.access.bullet.mail.mud.yahoo.com with NNFMP; 07 Dec 2011 15:43:51 -0000 4, 39 -- Received: from [98.138.207.10] by tm10.access.bullet.mail.mud.yahoo.com with NNFMP; 07 Dec 2011 15:43:51 -0000 4, 39 -- Received: from [127.0.0.1] by smtp103.biz.mail.ne1.yahoo.com with NNFMP; 07 Dec 2011 15:43:51 -0000 4, 39 -- X-Yahoo-Newman-Id: 415649.91694.bm-at-smtp103.biz.mail.ne1.yahoo.com 4, 39 -- X-Yahoo-Newman-Property: ymail-3 4, 39 -- X-YMail-OSG: SzNx04EVM1ku7JV29BWsqzoHjkDqUofZ8fApH.0Zo85sCma 4, 39 -- OmHA.WhUn6BN6bf2ddhf4L4UVzpm2ZiEHuNAlH6m0p6uPhhAmKGkHzHrJauB 4, 39 -- kJBXYJfGN7BH1JE05sVUndf2u6rmhDs3LyBOVEzPrg8TBAAA.pVWbHW74Qzn 4, 39 -- 85wbb_UDCt2f51PFlL.YZLa5KL2FSXRhRc1dKZaRtHLLU8hLZYee71meY6Kg 4, 39 -- PWh5UKST9h_kK3m9rnbR1UXOy.c9etyAPwx8wmXPX5ojKtgR8Oe91AggqP7z 4, 39 -- f3gZStcgzl75NmZFUsp5V7QrnZUHfWKSo_xH8vJTv46vucSu7BFV_3WgGjZO 4, 39 -- diqxtRPHktdCiHVoJKCEkZt36abZfpTsSySKjBvk_vzPS5w8SqklKFxhdl4D 4, 39 -- dwgS2BLACkrw20lJ3KtGDwI9tfV7ikZN3GbhYjOwlV0uxmiLFjC0rpLVWhjD 4, 39 -- D6P0l.jkNwmoF3N_nNyCAzeHI6DZ8SiPEEVtTnHvHHoWlSUqP1Es2BjLgdFw 4, 39 -- 9yUL2xSJwbSPLE27CD_WGB_QlgxXdQb8TxG1J8pCVKhg68yBEwn.mRcQ8ZLS 4, 39 -- 9MGz6Ju8xeo3l_0BpUP8VuDZFrGWaz2qfLjvSqvbKDBalHvja6tBfXTB4SA0 4, 39 -- k0mM6aX.klVwFuokK97NpaenxT8EQICDBaQnZHEDoWN_8W717tKqdBlxtVIM 4, 39 -- RkLpN 4, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 39 -- Received: from [192.168.1.127] (vray-at-71.174.120.150 with plain) 4, 39 -- by smtp103.biz.mail.ne1.yahoo.com with SMTP; 07 Dec 2011 07:43:51 -0800 PST 4, 39 -- Message-ID: {4EDF89BA.3080802-at-partbeamsystech.com} 4, 39 -- Date: Wed, 07 Dec 2011 10:43:54 -0500 4, 39 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 39 -- Reply-To: vray-at-partbeamsystech.com 4, 39 -- Organization: PBS&T 4, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 4, 39 -- MIME-Version: 1.0 4, 39 -- To: opmills-at-mtu.edu 4, 39 -- CC: microscopy-at-microscopy.com 4, 39 -- Subject: Re: [Microscopy] Materials: Gallistan in SEM 4, 39 -- References: {201112071354.pB7DsmHZ025164-at-ns.microscopy.com} 4, 39 -- In-Reply-To: {201112071354.pB7DsmHZ025164-at-ns.microscopy.com} 4, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 39 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I am not a metallurgist either, but dealt quite a bit with construction of vacuum hardware.
Based on my experience, which is always limited and often incorrect, behavior of the alloyed metals in vacuum has tendency to follow behavior of the components. If Zinc is not a vacuum-compatible then it will outgass from bronzes with high Zn content. If Al and Cu are vacuum-compatible then Al brass is excellent UHV material. etc... I can't claim that this rule of thumb works always, but most of the times it does.
You were specifically asking about outgassing - based on the components of the Gallistan it should not outgass in high vacuum. I did not test this myself and there is always a possibility that I may be wrong, but I feel confident enough to the point that I would load Gallistan into my own SEM without hesitation. And in my particular case - if SEM is damages then I would have to pay for parts to repair it from my own pocket!
I just took a quick look - the only reference on vapor pressure on Gallistan that I find only seem to suggest that its vapor pressure at 500C is below 10-8Torr - should be even lower at room temperature.
http://en.wikipedia.org/wiki/Galinstan
The MSDS which you sent gives the same information, and 10-8 is lower then in any commonly available HV SEM
However you will need to take precautions to avoid spill of the liquid Gallistan inside of the SEM chamber. I would not bring in large quantities of it, just for the fear of accidentally spilling it. Maybe drill tiny blind hole in the stab to hold a drop?
The fact that it wets/etches almost everything and forms amalgam with Al means that if you are planning on doing EDX then you may want to place a drop of it in some kind of PTFE (grounding will need to be addresse) or maybe graphite holder. I would go for graphite - it is available and machinable, thus should be easy to glue a piece of graphite on SEM stab and drill a small hole into the graphite for holding a drop of liquid Gallistan.
Other approach would be to rig your SEM with Peltier stage and freeze the monster.
P.S. I do not see any problem with being wrong from time to time, so there are no issues with distributing messages.
Cheers :)
Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 12/7/2011 1:14 PM, Monson, Frederick wrote: } Afternoon Valery, } } } From what I see, [(MSDS) http://www.rgmd.com/msds/msds.pdf], it appears to 'eat' or 'wet' lots of materials like 'skin oil' and 'firm metals', one of which is aluminum, and perhaps it would 'bind' or 'wet' the nickel film on the inside of my cast aluminum chamber (FEI Quanta 400 ESEM). } } I am not a metallurgist, but this MSDS appears, toward the end, to get down to the real non-biological business of the stuff. } } Also, with respect, it is apparently not about the components, it's about the properties of the mixture or 'solution'. } } I have heard of using Wood's Metal to fill microcracks in concrete prior to SEM. } } Gallinstan is conductive, so if a small drop were on a piece of grounded copper, one might get away with it, but is copper a 'firm' metal in this context (and on an Al stub)? } } Sorry for the disaggreement, but I thought there was much more to the story. } } Regards, } } Fred Monson } } P.S. I respect your rep, so I have sent this to you for distribution as you think reasonable. } } Frederick C. Monson, PhD } Technical Director } Center for Microanalysis and Imaging, Research and Training (CMIRT) } Schmucker Science Center South } West Chester University, West Chester, PA 19383 } 610-738-0437 } } Home Page: http://cmirt.wcupa.edu } Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl } } =============================================================================== } -----Original Message----- } From: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com] } Sent: Wednesday, December 07, 2011 10:55 AM } To: Monson, Frederick } Subject: [Microscopy] Re: Materials: Gallistan in SEM } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } In - Indium is UHV compatible } Ga - Gallium is vacuum-compatible at least till 10-9Torr } Sn - Tin (as metal) should be UHV compatible } } Alloy has low melting temperature, but I'd expect that vapor pressure at } room temperature would still be much lower then 10-8Torr - good enough } for high-vacuum SEM... } } Cheers :) } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 12/7/2011 8:54 AM, opmills-at-mtu.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Good morning. } } } } I've been asked to image Gallistan (Gallium, Tin, Indium) in our SEM. Note that the SEM is not a variable pressure system. Should I be concerned about outgassing and contamination of the specimen chamber? } } } } Thanks } } } } Owen Mills } } Michigan Tech University } } Houghton, MI } } } } ==============================Original Headers============================== } } 4, 20 -- From prvs=315ad1d10=opmills-at-mtu.edu Wed Dec 7 07:53:45 2011 } } 4, 20 -- Received: from mail.mtu.edu (mx1.mtu.edu [141.219.73.15]) } } 4, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB7DrjGU023864 } } 4, 20 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 07:53:45 -0600 } } 4, 20 -- X-IronPort-Anti-Spam-Filtered: true } } 4, 20 -- X-IronPort-Anti-Spam-Result: AvsEAOhu306N20GI/2dsb2JhbABDqlOBBYIzgX2IHJZflkWJBYpRYwSILp5+ } } 4, 20 -- X-IronPort-AV: E=Sophos;i="4.71,313,1320642000"; } } 4, 20 -- d="scan'208";a="476848913" } } 4, 20 -- Received: from e12-0626-fac.coe.ad.mtu.edu ([141.219.65.136]) } } 4, 20 -- by email.mtu.edu with ESMTP/TLS/AES128-SHA; 07 Dec 2011 08:53:44 -0500 } } 4, 20 -- From: "Owen P. Mills" {opmills-at-mtu.edu} } } 4, 20 -- Content-Type: text/plain; charset=us-ascii } } 4, 20 -- Subject: Materials: Gallistan in SEM } } 4, 20 -- Date: Wed, 7 Dec 2011 08:53:44 -0500 } } 4, 20 -- Message-Id: {F61531C1-B0F0-4F77-8C91-726860D3A2B3-at-mtu.edu} } } 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} } } 4, 20 -- Mime-Version: 1.0 (Apple Message framework v1084) } } 4, 20 -- X-Mailer: Apple Mail (2.1084) } } 4, 20 -- Content-Transfer-Encoding: 8bit } } 4, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB7DrjGU023864 } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 4, 39 -- From vray-at-partbeamsystech.com Wed Dec 7 09:43:52 2011 } 4, 39 -- Received: from nm27.access.bullet.mail.mud.yahoo.com (nm27.access.bullet.mail.mud.yahoo.com [66.94.237.92]) } 4, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pB7Fhp85010562 } 4, 39 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 09:43:52 -0600 } 4, 39 -- Received: from [66.94.237.199] by nm27.access.bullet.mail.mud.yahoo.com with NNFMP; 07 Dec 2011 15:43:51 -0000 } 4, 39 -- Received: from [98.138.207.10] by tm10.access.bullet.mail.mud.yahoo.com with NNFMP; 07 Dec 2011 15:43:51 -0000 } 4, 39 -- Received: from [127.0.0.1] by smtp103.biz.mail.ne1.yahoo.com with NNFMP; 07 Dec 2011 15:43:51 -0000 } 4, 39 -- X-Yahoo-Newman-Id: 415649.91694.bm-at-smtp103.biz.mail.ne1.yahoo.com } 4, 39 -- X-Yahoo-Newman-Property: ymail-3 } 4, 39 -- X-YMail-OSG: SzNx04EVM1ku7JV29BWsqzoHjkDqUofZ8fApH.0Zo85sCma } 4, 39 -- OmHA.WhUn6BN6bf2ddhf4L4UVzpm2ZiEHuNAlH6m0p6uPhhAmKGkHzHrJauB } 4, 39 -- kJBXYJfGN7BH1JE05sVUndf2u6rmhDs3LyBOVEzPrg8TBAAA.pVWbHW74Qzn } 4, 39 -- 85wbb_UDCt2f51PFlL.YZLa5KL2FSXRhRc1dKZaRtHLLU8hLZYee71meY6Kg } 4, 39 -- PWh5UKST9h_kK3m9rnbR1UXOy.c9etyAPwx8wmXPX5ojKtgR8Oe91AggqP7z } 4, 39 -- f3gZStcgzl75NmZFUsp5V7QrnZUHfWKSo_xH8vJTv46vucSu7BFV_3WgGjZO } 4, 39 -- diqxtRPHktdCiHVoJKCEkZt36abZfpTsSySKjBvk_vzPS5w8SqklKFxhdl4D } 4, 39 -- dwgS2BLACkrw20lJ3KtGDwI9tfV7ikZN3GbhYjOwlV0uxmiLFjC0rpLVWhjD } 4, 39 -- D6P0l.jkNwmoF3N_nNyCAzeHI6DZ8SiPEEVtTnHvHHoWlSUqP1Es2BjLgdFw } 4, 39 -- 9yUL2xSJwbSPLE27CD_WGB_QlgxXdQb8TxG1J8pCVKhg68yBEwn.mRcQ8ZLS } 4, 39 -- 9MGz6Ju8xeo3l_0BpUP8VuDZFrGWaz2qfLjvSqvbKDBalHvja6tBfXTB4SA0 } 4, 39 -- k0mM6aX.klVwFuokK97NpaenxT8EQICDBaQnZHEDoWN_8W717tKqdBlxtVIM } 4, 39 -- RkLpN } 4, 39 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- } 4, 39 -- Received: from [192.168.1.127] (vray-at-71.174.120.150 with plain) } 4, 39 -- by smtp103.biz.mail.ne1.yahoo.com with SMTP; 07 Dec 2011 07:43:51 -0800 PST } 4, 39 -- Message-ID: {4EDF89BA.3080802-at-partbeamsystech.com} } 4, 39 -- Date: Wed, 07 Dec 2011 10:43:54 -0500 } 4, 39 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} } 4, 39 -- Reply-To: vray-at-partbeamsystech.com } 4, 39 -- Organization: PBS&T } 4, 39 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 } 4, 39 -- MIME-Version: 1.0 } 4, 39 -- To: opmills-at-mtu.edu } 4, 39 -- CC: microscopy-at-microscopy.com } 4, 39 -- Subject: Re: [Microscopy] Materials: Gallistan in SEM } 4, 39 -- References: {201112071354.pB7DsmHZ025164-at-ns.microscopy.com} } 4, 39 -- In-Reply-To: {201112071354.pB7DsmHZ025164-at-ns.microscopy.com} } 4, 39 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 4, 39 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 14, 44 -- From vray-at-partbeamsystech.com Wed Dec 7 13:13:54 2011 14, 44 -- Received: from nm6-vm0.access.bullet.mail.mud.yahoo.com (nm6-vm0.access.bullet.mail.mud.yahoo.com [66.94.237.158]) 14, 44 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pB7JDrBo002329 14, 44 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 13:13:54 -0600 14, 44 -- Received: from [66.94.237.200] by nm6.access.bullet.mail.mud.yahoo.com with NNFMP; 07 Dec 2011 19:13:53 -0000 14, 44 -- Received: from [98.138.207.9] by tm11.access.bullet.mail.mud.yahoo.com with NNFMP; 07 Dec 2011 19:13:53 -0000 14, 44 -- Received: from [127.0.0.1] by smtp102.biz.mail.ne1.yahoo.com with NNFMP; 07 Dec 2011 19:13:53 -0000 14, 44 -- X-Yahoo-Newman-Id: 368267.10682.bm-at-smtp102.biz.mail.ne1.yahoo.com 14, 44 -- X-Yahoo-Newman-Property: ymail-3 14, 44 -- X-YMail-OSG: p9Y99SIVM1kN.2m6LOAHkhG2ia1HM198lopT02T7ELOaq7g 14, 44 -- UMhlfCCJTETjDevp65NlBE6y41XWuwJi7acHoRKZZMHdRg7Cxk_7yXuQI5Pw 14, 44 -- SO1YKv5RHRZiS38IQYZlP2DM3X4qV_hMntb_p47iyNqBt0MJsq0YLOd7GKO4 14, 44 -- _kbmjcOm2AYKxLlrmVeTbNnaKghFqN1gcM8YJB6UAH1F7Q0zCNbr4OhMSFX9 14, 44 -- wcxDG8uGk0y4r4yNqF6Ud4U2eKnjaZSUwqGdIR11llxk54r1tZt1veyMjd.I 14, 44 -- cko.YYHd4R46ExEQgchywsSoViGjHpLNw_7RvGApIis0bb6_A.u_8TevyAVC 14, 44 -- BEFLCKPlzAM04pxDH.VkcBz3BV0kx_MgKC1EXSVLQD6dCu4AmwWUqdVLtT1f 14, 44 -- 30F5m_R1va200fcm4wPWee0kOYKHxbcW1nJY44mt8Tj_NBPK.ViVH1TDnkR1 14, 44 -- ubmMk8d5o0AhlYn9z1sKBzrJwwHultswZibCX0LYJtElArsANIsQdjr.zPbW 14, 44 -- Kjre7_dc0resOQz5Ovy3Vrorc0pf4gxSRw62GpdpLDH_TTF6QTyAOoJSmOWG 14, 44 -- dynGdr1wMG5W4me9B0xeNPOIDfRfTKw71KheQpDzxboTwCqcKC.DTAIqsdCd 14, 44 -- bulHplzBXa1gScgk3Cxj4_qHzcxAMjGInH.AngvbsXZxMkciWnUfZDypqgBG 14, 44 -- e.LDyBdblBMgmdaks_dInFUu6owpT42PF5WKg_Za5MtrZIqWrIyYJ5u0hGZ7 14, 44 -- 1c3N.1CUDnTcImIoX8tsWBYvt74Zcbsm1ZMXZM_NxyrjW8bEOGynhSJsLkPD 14, 44 -- hrPTnP.YEEJB0Y_7zk4YjBRRA9I7T1zuvdvcxfnyYveObqbekZlmzj6mYftM 14, 44 -- 2v90wYRWSbkdZ_SR_fq31Ht_nrATKncoM1sXbPQYnwoEdpJHnpET8mN7zvCe 14, 44 -- T4.lZLL5Ir5biDbl8CcEhJyavoUnOMY3EHhr1gI9xqhvAbFSLfWF3OgJSPI3 14, 44 -- 66saAr5kbN5l7ywxnrGoL__FIw1dLz5la6M4bT7i_Xr1eY.HLiWo5 14, 44 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 14, 44 -- Received: from [192.168.1.127] (vray-at-71.174.120.150 with plain) 14, 44 -- by smtp102.biz.mail.ne1.yahoo.com with SMTP; 07 Dec 2011 11:13:53 -0800 PST 14, 44 -- Message-ID: {4EDFBAF2.7010805-at-partbeamsystech.com} 14, 44 -- Date: Wed, 07 Dec 2011 14:13:54 -0500 14, 44 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 14, 44 -- Reply-To: vray-at-partbeamsystech.com 14, 44 -- Organization: PBS&T 14, 44 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 14, 44 -- MIME-Version: 1.0 14, 44 -- To: "Monson, Frederick" {FMonson-at-wcupa.edu} 14, 44 -- CC: microscopy-at-microscopy.com 14, 44 -- Subject: Re: [Microscopy] Re: Materials: Gallistan in SEM 14, 44 -- References: {201112071554.pB7Fswui022998-at-ns.microscopy.com} {899EAA40AE6E47488CE0637489EFB30C10B528-at-WCU-XCH-03.PASSHE.LCL} 14, 44 -- In-Reply-To: {899EAA40AE6E47488CE0637489EFB30C10B528-at-WCU-XCH-03.PASSHE.LCL} 14, 44 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 14, 44 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
The peak you see is very close to O+Si sum peak, and I strongly believe it is the O+Si peak. You can try to decrease intensity of excitation beam until Si sum peak disappear, then O+Si peak should disappear also. Vladimir
Vladimir M. Dusevich, Ph.D. Electron Microscope Lab Director 371 School of Dentistry 650 E. 25th Street Kansas City, MO 64108-2784
} -----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com } [mailto:microscopylistserver-noreply-at-microscopy.com] } Sent: Wednesday, December 07, 2011 2:19 AM } To: Dusevich, Vladimir } Subject: [Microscopy] viaWWW:EDX Spectrum of Silicone Rubber } } } } } ----------------------------------------------------------------------- } ----- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ----------------------------------------------------------------------- } ----- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } ----------------------------------------------------------------------- } ---- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both donk-at-ardl.com as well as the MIcroscopy } Listserver } ----------------------------------------------------------------------- } ---- } } Email: donk-at-ardl.com Name: Don Kierstead } } Organization: Akron Rubber Development Laboratories, Inc. } } Title-Subject: [Filtered] RE: EDX Spectrum of Silicone Rubber } } Message: I am having trouble with interpreting an EDX spectrum of a } silicone rubber sample. The elements detected are carbon (8295 } counts)), oxygen (13,483 counts), and silicon (90549). So, as you can } see, I have a really big silicon peak with oxygen being a moderate } sized } peak and carbon small. The problem I am having is that I'm getting a } small (910 cnt) peak at 2.235KeV that doesn't really line up with any } specific x-rays on any of the charts and does not appear to be an } escape } or sum peak for any of the elements present. The closest } identification } would be either thallium or mercury, but it is not likely They would be } present in the rubber. I do get a good sized silicon sum peak at } 3.465KeV (1136 cnt.). The question to the list is whether or not this } peak is an artifact or perhaps a silicon x-ray peak that is not listed } on the charts. I am operating an IXRF EDS 2008 computer software. Any } feedback would be greatly appreciated! } } Login Host: 66.83.169.2 } ----------------------------------------------------------------------- } ---- } } } } ==============================Original } Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Wed Dec 7 } 02:16:52 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP } id pB78Gqo1032251 } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 02:16:52 - } 0600 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id CDB69451B86 } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 02:16:52 } -0600 (CST) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd- } new, port 10024) } 8, 25 -- with ESMTP id E6g8gQl1UAJw for {microscopy-at-microscopy.com} ; } 8, 25 -- Wed, 7 Dec 2011 02:16:46 -0600 (CST) } 8, 25 -- Received: from NestorPwrBk.local } (83.138.221.115.dyn.user.ono.com [83.138.221.115]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id 34946451B7B } 8, 25 -- for {microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 02:16:44 } -0600 (CST) } 8, 25 -- Message-ID: {4EDF20E9.3060500-at-microscopy.com} } 8, 25 -- Date: Wed, 07 Dec 2011 09:16:41 +0100 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver- } noreply-at-microscopy.com} } 8, 25 -- Reply-To: donk-at-ardl.com } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; } en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:EDX Spectrum of Silicone Rubber } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
==============================Original Headers============================== 5, 29 -- From DusevichV-at-umkc.edu Wed Dec 7 13:29:01 2011 5, 29 -- Received: from mxnip01.um.umsystem.edu (mxnip01.um.umsystem.edu [209.106.229.21]) 5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB7JT1ew019519 5, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 7 Dec 2011 13:29:01 -0600 5, 29 -- X-IronPort-Anti-Spam-Filtered: true 5, 29 -- X-IronPort-Anti-Spam-Result: AskBAL+9306Dl3nN/2dsb2JhbABDmiOIfoQ4ghFrfgeBcgEBBToRAQI3BAIBCBECAQEBAQ0KAQcJByUMARQJCAEBBAoJCBUEh26pRwGEIokFiBgBgjhjBIgunn8 5, 29 -- Received: from um-msxhub05.um.umsystem.edu ([131.151.121.205]) 5, 29 -- by mxnip01-missouri-out.um.umsystem.edu with ESMTP; 07 Dec 2011 13:29:01 -0600 5, 29 -- Received: from um-email06.um.umsystem.edu ([169.254.1.27]) by 5, 29 -- UM-MSXHUB05.um.umsystem.edu ([131.151.121.205]) with mapi; Wed, 7 Dec 2011 5, 29 -- 13:29:00 -0600 5, 29 -- From: "Dusevich, Vladimir" {DusevichV-at-umkc.edu} 5, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 5, 29 -- Date: Wed, 7 Dec 2011 13:29:02 -0600 5, 29 -- Subject: RE: [Microscopy] viaWWW:EDX Spectrum of Silicone Rubber 5, 29 -- Thread-Topic: [Microscopy] viaWWW:EDX Spectrum of Silicone Rubber 5, 29 -- Thread-Index: Acy0uNYSx6ogj1L7STWfghUFWvUlzgAXQw+w 5, 29 -- Message-ID: {5079A076208DF9428FC9A6D2129D78A3B7A7F5C6B9-at-UM-EMAIL06.um.umsystem.edu} 5, 29 -- References: {201112070818.pB78IdNN000522-at-ns.microscopy.com} 5, 29 -- In-Reply-To: {201112070818.pB78IdNN000522-at-ns.microscopy.com} 5, 29 -- Accept-Language: en-US 5, 29 -- Content-Language: en-US 5, 29 -- X-MS-Has-Attach: 5, 29 -- X-MS-TNEF-Correlator: 5, 29 -- acceptlanguage: en-US 5, 29 -- Content-Type: text/plain; charset="us-ascii" 5, 29 -- MIME-Version: 1.0 5, 29 -- Content-Transfer-Encoding: 8bit 5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB7JT1ew019519 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both eggert-at-mikroanalytik.de as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: eggert-at-mikroanalytik.de Name: Frank Eggert
Organization: EDAX
Title-Subject: [Filtered] EDX Spectrum of Silicone Rubber
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both stevebuckingham-at-comcast.net as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: stevebuckingham-at-comcast.net Name: Steve Buckingham
Organization: Satki3
Title-Subject: [Filtered] Amray 1860 FE Scanning Electron Microscope support
Message: I'm thinking of purchasing a used Amray 1860 FE Scanning Electron Microscope but I am concerned that there may not be support or parts still available for these systems. The system is in full working condition right now and can be demonstrated for performance. However, I would be interested to know what kind of support still exists for repair of these systems along with information on the availability of parts. Many Thanks, Steve
The word on the street is that service for AMRAY SEMs may be available from North Billerica. Contact these guys:
http://www.semtechsolutions.com/
Cheers :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 12/9/2011 4:04 AM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both stevebuckingham-at-comcast.net as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: stevebuckingham-at-comcast.net Name: Steve Buckingham } } Organization: Satki3 } } Title-Subject: [Filtered] Amray 1860 FE Scanning Electron Microscope support } } Message: I'm thinking of purchasing a used Amray 1860 FE Scanning } Electron Microscope but I am concerned that there may not be support or } parts still available for these systems. The system is in full working } condition right now and can be demonstrated for performance. } However, I would be interested to know what kind of support still exists } for repair of these systems along with information on the availability } of parts. } Many Thanks, } Steve } } Login Host: 68.61.156.255 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 9 03:01:32 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB991WD8025987 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:32 -0600 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 88550453501 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:32 -0600 (CST) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 8, 25 -- with ESMTP id 27dDULW1os2z for {microscopy-at-microscopy.com} ; } 8, 25 -- Fri, 9 Dec 2011 03:01:31 -0600 (CST) } 8, 25 -- Received: from NestorPwrBk.local (83.138.221.115.dyn.user.ono.com [83.138.221.115]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id C1D1C4534F6 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:30 -0600 (CST) } 8, 25 -- Message-ID: {4EE1CE69.9000702-at-microscopy.com} } 8, 25 -- Date: Fri, 09 Dec 2011 10:01:29 +0100 } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: stevebuckingham-at-comcast.net } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: viaWWW:Amray 1860 FE Scanning Electron Microscope support } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 4, 42 -- From vray-at-partbeamsystech.com Fri Dec 9 06:23:37 2011 4, 42 -- Received: from nm22.access.bullet.mail.mud.yahoo.com (nm22.access.bullet.mail.mud.yahoo.com [66.94.237.223]) 4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pB9CNaN2014740 4, 42 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 06:23:37 -0600 4, 42 -- Received: from [66.94.237.195] by nm22.access.bullet.mail.mud.yahoo.com with NNFMP; 09 Dec 2011 12:23:36 -0000 4, 42 -- Received: from [98.137.12.183] by tm6.access.bullet.mail.mud.yahoo.com with NNFMP; 09 Dec 2011 12:23:36 -0000 4, 42 -- Received: from [127.0.0.1] by smtp108.biz.mail.gq1.yahoo.com with NNFMP; 09 Dec 2011 12:23:35 -0000 4, 42 -- X-Yahoo-Newman-Id: 949478.50181.bm-at-smtp108.biz.mail.gq1.yahoo.com 4, 42 -- X-Yahoo-Newman-Property: ymail-3 4, 42 -- X-YMail-OSG: Jv2rfYcVM1k..3c3COI_JOXrlV7iXfSRrJdym4mtvWgP3xR 4, 42 -- Q2f8qfE.iQ0bIB1JZ8naG7WrOARksh30Ug1bE7JaE9lhvEI4tLEA7ePUHrwN 4, 42 -- EHg3yCUQrv4y8YrH.RX7llAczKtvOQ9rPlp0kGE5OPgC1WFnfdwjA0ehfsWf 4, 42 -- nU0yFn7aeQsEGUIbD4E8i8k718CvMixLUA.fI07Ecm0hD5lJILNhrU1o4qhU 4, 42 -- XsZRQx9ICsnO4cYcbxwroWDxplQ3j7_sI8BJkLTSdNayuiI6MFjl4TZW3gz. 4, 42 -- Ge6nkB5EUWrFW5VfImGfvUgn_vAVqe9rhnAqwt3aN2xk68j3dLuDts2cQsgm 4, 42 -- N40T0TmU_.SvELRKYIwFsQDNoZIgaRCiIt0i9KiWypeoj3WwhMdBF8w08q7K 4, 42 -- QVSeKr3oFtu8DAS6VQaruHcRj9Ksh_aBHsvVS9mwe3BTtWfbUSs1ScUi9ZOt 4, 42 -- _QTRwhBRO0XkmNGnaQbSIIybkl8imBY4HhG9e_JdrLgxiUD7f_pAiND7dvl6 4, 42 -- eVlefJsqbDSifXElcC5PPGnyy2ZptOENt.hVm8NfWxOo_lmVDAmOqsnMafln 4, 42 -- nrVjPNH1NQQMio9RZHaH1K3C0Gm.R7DTEnI2c1ky_FUtZvp6V_WPaQ0XwaSw 4, 42 -- 4l68lE73Eg4nyeQY0BS2y6LhqFC84qg5xPwDYoM8OmUUznuy0wxeG8VclIth 4, 42 -- Fz8eVpbnPJ0DsjmnJGOhtHzudi2pmVT83BuzdoLrPuxSKfHnNURboZ2vIWVg 4, 42 -- MFWMFCsWm.a4cq0AG0pUf821j6diuWwMluxFpYgLerXn1kbph1VCTxi0WO88 4, 42 -- hcEWMv6cp.IDLkWC63PzOVY02a_2rQ4Pn 4, 42 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 4, 42 -- Received: from [192.168.2.2] (vray-at-96.233.126.242 with plain) 4, 42 -- by smtp108.biz.mail.gq1.yahoo.com with SMTP; 09 Dec 2011 04:23:35 -0800 PST 4, 42 -- Message-ID: {4EE1FDC6.8070202-at-partbeamsystech.com} 4, 42 -- Date: Fri, 09 Dec 2011 07:23:34 -0500 4, 42 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 4, 42 -- Reply-To: vray-at-partbeamsystech.com 4, 42 -- Organization: PBS&T 4, 42 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 4, 42 -- MIME-Version: 1.0 4, 42 -- To: stevebuckingham-at-comcast.net 4, 42 -- CC: microscopy-at-microscopy.com 4, 42 -- Subject: Re: [Microscopy] viaWWW:Amray 1860 FE Scanning Electron Microscope 4, 42 -- support 4, 42 -- References: {201112090904.pB994gGH027451-at-ns.microscopy.com} 4, 42 -- In-Reply-To: {201112090904.pB994gGH027451-at-ns.microscopy.com} 4, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 4, 42 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
Please note that I have already suggested going to the MSA certification website. But: is this sufficient for clinical/pathology certification in TEM?
} Date: Fri, 9 Dec 2011 08:03:56 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: Cathy Kuehner BEMT/MSA {Cathy.Kuehner-at-hcmed.org} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Friday, December 09, } 2011 at 08:03:55 AM. } } realname - Cathy Kuehner BEMT/MSA } Email - Cathy.Kuehner-at-hcmed.org } ORGANIZATION - Hennepin Co Medical Center } LOCATION - Minneapolis, Mn 55404, USA } SUBJECT_OF_QUESTION - Proficiency testing } QUESTION - The clinical/patholgy labs at our institution are } accredited by the College of American Pathologists. } For our next inspection CAP states that all accredited labs must } participate in a proficiency program. } We are having a difficult time finding a proficiency program for } TEM. CAP does not have program for EM. } Do know know how other diagnostic labs deal with this issue?
==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Fri Dec 9 10:13:41 2011 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB9GDfpM003272 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 10:13:41 -0600 4, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 4, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pB9GDbpl020891 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 11:13:40 -0500 4, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Fri, 9 Dec 4, 24 -- 2011 11:13:38 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Message-ID: {a06240808cb07e3b87f50-at-[141.209.160.249]} 4, 24 -- Date: Fri, 9 Dec 2011 11:13:35 -0500 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: clinical programs for EM proficiency 4, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 24 -- X-Originating-IP: [141.209.160.249] 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 4, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 02G74dEX4 - 418320540949 - 20111209 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
Electron Optics Service services the old Amray microscopes. The guy who runs it is Jimmy Fotinopoulos. They have a warehouse full of old Amrays that they use for parts. You could call him and see if he covers that model: 973 669 3134
My only interest is as a satisfied customer.
dj
On Fri, 9 Dec 2011, vray-at-partbeamsystech.com wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The word on the street is that service for AMRAY SEMs may be available } from North Billerica. Contact these guys: } } http://www.semtechsolutions.com/ } } Cheers :) } Valery Ray } ================================= } PBS&T, MEO Engineering Co., Inc. } 290 Broadway, Suite 298 } Methuen, MA 01844 USA } Phone: +1-978-296-5063 - leave a message with call-back number } US Mobile: +1-978-305-0479 } Skype: pbstmeo } E-mail: vray-at-partbeamsystech.com } Web: www.partbeamsystech.com } Web: www.freudlabs.com } } On 12/9/2011 4:04 AM, microscopylistserver-noreply-at-microscopy.com wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at } } http://microscopy.com/MicroscopyListserver/MLFormMail.html } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both stevebuckingham-at-comcast.net as well as the } } MIcroscopy Listserver } } --------------------------------------------------------------------------- } } } } Email: stevebuckingham-at-comcast.net Name: Steve Buckingham } } } } Organization: Satki3 } } } } Title-Subject: [Filtered] Amray 1860 FE Scanning Electron Microscope support } } } } Message: I'm thinking of purchasing a used Amray 1860 FE Scanning } } Electron Microscope but I am concerned that there may not be support or } } parts still available for these systems. The system is in full working } } condition right now and can be demonstrated for performance. } } However, I would be interested to know what kind of support still exists } } for repair of these systems along with information on the availability } } of parts. } } Many Thanks, } } Steve } } } } Login Host: 68.61.156.255 } } --------------------------------------------------------------------------- } } } } } } } } ==============================Original Headers============================== } } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 9 03:01:32 2011 } } 8, 25 -- Received: from znl.com ([206.69.208.20]) } } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB991WD8025987 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:32 -0600 } } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } } 8, 25 -- by znl.com (Postfix) with ESMTP id 88550453501 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:32 -0600 (CST) } } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } } 8, 25 -- Received: from znl.com ([127.0.0.1]) } } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } } 8, 25 -- with ESMTP id 27dDULW1os2z for {microscopy-at-microscopy.com} ; } } 8, 25 -- Fri, 9 Dec 2011 03:01:31 -0600 (CST) } } 8, 25 -- Received: from NestorPwrBk.local (83.138.221.115.dyn.user.ono.com [83.138.221.115]) } } 8, 25 -- by znl.com (Postfix) with ESMTPA id C1D1C4534F6 } } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:30 -0600 (CST) } } 8, 25 -- Message-ID: {4EE1CE69.9000702-at-microscopy.com} } } 8, 25 -- Date: Fri, 09 Dec 2011 10:01:29 +0100 } } 8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } } 8, 25 -- Reply-To: stevebuckingham-at-comcast.net } } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } } 8, 25 -- MIME-Version: 1.0 } } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } } 8, 25 -- Subject: viaWWW:Amray 1860 FE Scanning Electron Microscope support } } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } } 8, 25 -- Content-Transfer-Encoding: 7bit } } ==============================End of - Headers============================== } } } } ==============================Original Headers============================== } 4, 42 -- From vray-at-partbeamsystech.com Fri Dec 9 06:23:37 2011 } 4, 42 -- Received: from nm22.access.bullet.mail.mud.yahoo.com (nm22.access.bullet.mail.mud.yahoo.com [66.94.237.223]) } 4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pB9CNaN2014740 } 4, 42 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 06:23:37 -0600 } 4, 42 -- Received: from [66.94.237.195] by nm22.access.bullet.mail.mud.yahoo.com with NNFMP; 09 Dec 2011 12:23:36 -0000 } 4, 42 -- Received: from [98.137.12.183] by tm6.access.bullet.mail.mud.yahoo.com with NNFMP; 09 Dec 2011 12:23:36 -0000 } 4, 42 -- Received: from [127.0.0.1] by smtp108.biz.mail.gq1.yahoo.com with NNFMP; 09 Dec 2011 12:23:35 -0000 } 4, 42 -- X-Yahoo-Newman-Id: 949478.50181.bm-at-smtp108.biz.mail.gq1.yahoo.com } 4, 42 -- X-Yahoo-Newman-Property: ymail-3 } 4, 42 -- X-YMail-OSG: Jv2rfYcVM1k..3c3COI_JOXrlV7iXfSRrJdym4mtvWgP3xR } 4, 42 -- Q2f8qfE.iQ0bIB1JZ8naG7WrOARksh30Ug1bE7JaE9lhvEI4tLEA7ePUHrwN } 4, 42 -- EHg3yCUQrv4y8YrH.RX7llAczKtvOQ9rPlp0kGE5OPgC1WFnfdwjA0ehfsWf } 4, 42 -- nU0yFn7aeQsEGUIbD4E8i8k718CvMixLUA.fI07Ecm0hD5lJILNhrU1o4qhU } 4, 42 -- XsZRQx9ICsnO4cYcbxwroWDxplQ3j7_sI8BJkLTSdNayuiI6MFjl4TZW3gz. } 4, 42 -- Ge6nkB5EUWrFW5VfImGfvUgn_vAVqe9rhnAqwt3aN2xk68j3dLuDts2cQsgm } 4, 42 -- N40T0TmU_.SvELRKYIwFsQDNoZIgaRCiIt0i9KiWypeoj3WwhMdBF8w08q7K } 4, 42 -- QVSeKr3oFtu8DAS6VQaruHcRj9Ksh_aBHsvVS9mwe3BTtWfbUSs1ScUi9ZOt } 4, 42 -- _QTRwhBRO0XkmNGnaQbSIIybkl8imBY4HhG9e_JdrLgxiUD7f_pAiND7dvl6 } 4, 42 -- eVlefJsqbDSifXElcC5PPGnyy2ZptOENt.hVm8NfWxOo_lmVDAmOqsnMafln } 4, 42 -- nrVjPNH1NQQMio9RZHaH1K3C0Gm.R7DTEnI2c1ky_FUtZvp6V_WPaQ0XwaSw } 4, 42 -- 4l68lE73Eg4nyeQY0BS2y6LhqFC84qg5xPwDYoM8OmUUznuy0wxeG8VclIth } 4, 42 -- Fz8eVpbnPJ0DsjmnJGOhtHzudi2pmVT83BuzdoLrPuxSKfHnNURboZ2vIWVg } 4, 42 -- MFWMFCsWm.a4cq0AG0pUf821j6diuWwMluxFpYgLerXn1kbph1VCTxi0WO88 } 4, 42 -- hcEWMv6cp.IDLkWC63PzOVY02a_2rQ4Pn } 4, 42 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- } 4, 42 -- Received: from [192.168.2.2] (vray-at-96.233.126.242 with plain) } 4, 42 -- by smtp108.biz.mail.gq1.yahoo.com with SMTP; 09 Dec 2011 04:23:35 -0800 PST } 4, 42 -- Message-ID: {4EE1FDC6.8070202-at-partbeamsystech.com} } 4, 42 -- Date: Fri, 09 Dec 2011 07:23:34 -0500 } 4, 42 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} } 4, 42 -- Reply-To: vray-at-partbeamsystech.com } 4, 42 -- Organization: PBS&T } 4, 42 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 } 4, 42 -- MIME-Version: 1.0 } 4, 42 -- To: stevebuckingham-at-comcast.net } 4, 42 -- CC: microscopy-at-microscopy.com } 4, 42 -- Subject: Re: [Microscopy] viaWWW:Amray 1860 FE Scanning Electron Microscope } 4, 42 -- support } 4, 42 -- References: {201112090904.pB994gGH027451-at-ns.microscopy.com} } 4, 42 -- In-Reply-To: {201112090904.pB994gGH027451-at-ns.microscopy.com} } 4, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 4, 42 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 21 -- From dljones-at-bestweb.net Fri Dec 9 18:37:45 2011 5, 21 -- Received: from mta4.srv.hcvlny.cv.net (mta4.srv.hcvlny.cv.net [167.206.4.199]) 5, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBA0biIl030655 5, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 18:37:44 -0600 5, 21 -- Received: from marcy (ool-4356ff7a.dyn.optonline.net [67.86.255.122]) 5, 21 -- by mta4.srv.hcvlny.cv.net 5, 21 -- (Sun Java System Messaging Server 6.2-8.04 (built Feb 28 2007)) 5, 21 -- with ESMTP id {0LVY00F66PQUVHT0-at-mta4.srv.hcvlny.cv.net} for 5, 21 -- Microscopy-at-microscopy.com; Fri, 09 Dec 2011 19:37:43 -0500 (EST) 5, 21 -- Date: Fri, 09 Dec 2011 19:37:53 -0500 (Eastern Standard Time) 5, 21 -- From: David Jones {dljones-at-bestweb.net} 5, 21 -- Subject: Re: [Microscopy] Re: viaWWW:Amray 1860 FE Scanning Electron Microscope 5, 21 -- In-reply-to: {201112091228.pB9CSNUU019364-at-ns.microscopy.com} 5, 21 -- X-X-Sender: dljones-at-imap.bestweb.net 5, 21 -- To: Microscopy-at-microscopy.com 5, 21 -- Message-id: {alpine.WNT.2.00.1112091926150.1748-at-marcy} 5, 21 -- MIME-version: 1.0 5, 21 -- Content-type: TEXT/PLAIN; format=flowed; charset=US-ASCII 5, 21 -- Content-transfer-encoding: 7BIT 5, 21 -- References: {201112091228.pB9CSNUU019364-at-ns.microscopy.com} 5, 21 -- User-Agent: Alpine 2.00 (WNT 1167 2008-08-23) ==============================End of - Headers==============================
Dear group, We have electronic design and programming job openings in central part of NC. Development and pre-manufacturing stage, some research. Full time and part time. Some remote programming and hardware development may be available. For details, send resume to me directly with you questions. Thanks
==============================Original Headers============================== 2, 23 -- From uti-at-umbtech.com Fri Dec 9 19:13:41 2011 2, 23 -- Received: from dns.dns182.com (dns.dns182.com [174.121.243.225]) 2, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBA1DfYo015313 2, 23 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 19:13:41 -0600 2, 23 -- Message-Id: {201112100113.pBA1DfYo015313-at-ns.microscopy.com} 2, 23 -- Received: from [67.238.137.225] (helo=Qu-PC.umbtech.com) 2, 23 -- by dns.dns182.com with esmtpsa (TLSv1:AES256-SHA:256) 2, 23 -- (Exim 4.69) 2, 23 -- (envelope-from {uti-at-umbtech.com} ) 2, 23 -- id 1RZBVS-0005b2-Sk 2, 23 -- for Microscopy-at-microscopy.com; Fri, 09 Dec 2011 20:13:39 -0500 2, 23 -- X-Mailer: QUALCOMM Windows Eudora Version 7.1.0.9 2, 23 -- Date: Fri, 09 Dec 2011 20:13:35 -0500 2, 23 -- To: Microscopy-at-microscopy.com 2, 23 -- From: UTI {uti-at-umbtech.com} 2, 23 -- Subject: North Carolina - Job opportunity 2, 23 -- Mime-Version: 1.0 2, 23 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 23 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report 2, 23 -- X-AntiAbuse: Primary Hostname - dns.dns182.com 2, 23 -- X-AntiAbuse: Original Domain - microscopy.com 2, 23 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12] 2, 23 -- X-AntiAbuse: Sender Address Domain - umbtech.com ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both {PRICE-at-gw.med.sc.edu} as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: {PRICE-at-gw.med.sc.edu} Name: "Bob Price" Organization: University of South Carolina
Title-Subject: [Filtered] Confocal Workshop
Question: The University of South Carolina Instrumentation Resource Facility and the South Carolina EPSCoR (NSF) and INBRE (NIH) programs will be hosting a hands on workshop in Basic Confocal Microscopy from June 18-21. Full details concerning the workshop and registration information can be found at http://www.scepscor.org/outreach/workshops/confocal-microscopy/home.html The workshop will consist of both lectures and laboratory sessions and is directed towards beginning and intermediate level users of confocal microscopy. Topics covered will include the basics of fluorescence and selection of probes, specimen fixation and staining, proper set up of operating parameters for collection of images, composition of digital confocal images, the use of image enhancement and analysis programs such as Photoshop and Metamorph for confocal images, and the use of 3-D reconstruction programs such as AMIRA. Instructors for the workshop will include Dr. Jay Jerome from Vanderbilt University, Dr. John Mackenzie from North Carolina State University, Dr. Tom Trusk from the Medical University of South Carolina, and Drs. Bob Price and John Fuseler from the USC School of Medicine. A variety of confocal microscopes including the BD CARV spinning disk, Leica, Nikon, Olympus, and Zeiss systems are scheduled to be on hand for the laboratory sessions. Applications experts from each of the companies will also be on hand for discussion of their specific systems. Participants are encouraged to bring their own specimens for sample preparation and imaging sessions, but a variety of cell culture and tissue sections will also be available for laboratory sessions on fluorescent staining and imaging. For further information or questions please contact Bob Price (Price-at-med.sc.edu) Workshop on Basic Confocal Microscopy Monday June 18, 2007 8:30-9:00 Registration and Continental Breakfast, Bldg 1, Room B59 USC School of Medicine 9:00-9:15 Welcome and Logistics 9:15-10:15 Introduction and Overview of Confocal Microscopy: Jay Jerome (Vanderbilt University) and Bob Price (USC School of Medicine) 10:15-10:45 Break and Discussion 10:45-12:00 Basics of Fluorescence, Dye Characteristics: Jerome and Price 12:00-1:00 Lunch (Provided) and discussion 1:00-3:00 Specimen Preparation: Jerome and Price 3:00-3:30 Break and Discussion 3:30-?? Lab * Specimen Preparation and processing: Processing of own samples or samples will be provided. Fixation through overnight primary antibody incubation Dinner on your own
Tuesday June 19, 2007 8:30-9:00 Continental Breakfast * Room B59 9:00-10:00 Lab - Wash specimens and secondary antibody incubation 10:00-10:30 Break and Discussion 10:30-12:00 Components, proper set up of operating parameters, and types of confocal microscopes: Jerome and Price 12:00-1:30 Lunch (Provided) and Specimen Processing 1:30-2:30 Digital Images in Confocal Microscopy * Jerome and Price 2:30-4:00 Lab * finish specimen processing 4:00-6:00 Time on Instruments (BD CARV, Leica, Nikon, Olympus, Zeiss systems are scheduled to be available) Dinner on your own
Wednesday June 20, 2007 8:30-9:00 Continental Breakfast * Room B59 9:00-12:00 Photoshop, etc with confocal images (John Mackenzie * North Carolina State University) 12:00-1:00 Lunch 1:00-5:00 Time on Confocal Instruments and Software 6:00-?? Reception on Lake Murray
Thursday June 21, 2007 8:30-9:00 Continental Breakfast * Room B59 9:00-11:00 3-D reconstruction of confocal data sets with AMIRA (Tom Trusk * Medical University of South Carolina) 11:00-12:00 Ethics in Use of Confocal Images, Available Resources, Discussion 12:00 - 4:00 Lunch and additional time on Confocal Instruments and Software (Photoshop, AMIRA, Metamorph)
Robert L. Price, PhD Research Professor and Director, Instrumentation Resource Facility School of Medicine, USC Bldg 1 Room B60 6439 Garner's Ferry Road Columbia, SC 29208
I inadvertently forwarded the Email below to the listserver. Sorry, it is an OLD Email for a workshop from several years ago. I neglected to check the date and when I saw in in my in box thought it was a new message for a new workshop.
Please do not fault Bob Price, it is my fault entirely.
Nestor
Your Friendly Neighborhood SysOp
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both {PRICE-at-gw.med.sc.edu} as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: {PRICE-at-gw.med.sc.edu} Name: "Bob Price" Organization: University of South Carolina
Title-Subject: [Filtered] Confocal Workshop
Question: The University of South Carolina Instrumentation Resource Facility and the South Carolina EPSCoR (NSF) and INBRE (NIH) programs will be hosting a hands on workshop in Basic Confocal Microscopy from June 18-21. Full details concerning the workshop and registration information can be found at http://www.scepscor.org/outreach/workshops/confocal-microscopy/home.html The workshop will consist of both lectures and laboratory sessions and is directed towards beginning and intermediate level users of confocal microscopy. Topics covered will include the basics of fluorescence and selection of probes, specimen fixation and staining, proper set up of operating parameters for collection of images, composition of digital confocal images, the use of image enhancement and analysis programs such as Photoshop and Metamorph for confocal images, and the use of 3-D reconstruction programs such as AMIRA. Instructors for the workshop will include Dr. Jay Jerome from Vanderbilt University, Dr. John Mackenzie from North Carolina State University, Dr. Tom Trusk from the Medical University of South Carolina, and Drs. Bob Price and John Fuseler from the USC School of Medicine. A variety of confocal microscopes including the BD CARV spinning disk, Leica, Nikon, Olympus, and Zeiss systems are scheduled to be on hand for the laboratory sessions. Applications experts from each of the companies will also be on hand for discussion of their specific systems. Participants are encouraged to bring their own specimens for sample preparation and imaging sessions, but a variety of cell culture and tissue sections will also be available for laboratory sessions on fluorescent staining and imaging. For further information or questions please contact Bob Price (Price-at-med.sc.edu) Workshop on Basic Confocal Microscopy Monday June 18, 2007 8:30-9:00 Registration and Continental Breakfast, Bldg 1, Room B59 USC School of Medicine 9:00-9:15 Welcome and Logistics 9:15-10:15 Introduction and Overview of Confocal Microscopy: Jay Jerome (Vanderbilt University) and Bob Price (USC School of Medicine) 10:15-10:45 Break and Discussion 10:45-12:00 Basics of Fluorescence, Dye Characteristics: Jerome and Price 12:00-1:00 Lunch (Provided) and discussion 1:00-3:00 Specimen Preparation: Jerome and Price 3:00-3:30 Break and Discussion 3:30-?? Lab * Specimen Preparation and processing: Processing of own samples or samples will be provided. Fixation through overnight primary antibody incubation Dinner on your own
Tuesday June 19, 2007 8:30-9:00 Continental Breakfast * Room B59 9:00-10:00 Lab - Wash specimens and secondary antibody incubation 10:00-10:30 Break and Discussion 10:30-12:00 Components, proper set up of operating parameters, and types of confocal microscopes: Jerome and Price 12:00-1:30 Lunch (Provided) and Specimen Processing 1:30-2:30 Digital Images in Confocal Microscopy * Jerome and Price 2:30-4:00 Lab * finish specimen processing 4:00-6:00 Time on Instruments (BD CARV, Leica, Nikon, Olympus, Zeiss systems are scheduled to be available) Dinner on your own
Wednesday June 20, 2007 8:30-9:00 Continental Breakfast * Room B59 9:00-12:00 Photoshop, etc with confocal images (John Mackenzie * North Carolina State University) 12:00-1:00 Lunch 1:00-5:00 Time on Confocal Instruments and Software 6:00-?? Reception on Lake Murray
Thursday June 21, 2007 8:30-9:00 Continental Breakfast * Room B59 9:00-11:00 3-D reconstruction of confocal data sets with AMIRA (Tom Trusk * Medical University of South Carolina) 11:00-12:00 Ethics in Use of Confocal Images, Available Resources, Discussion 12:00 - 4:00 Lunch and additional time on Confocal Instruments and Software (Photoshop, AMIRA, Metamorph)
Robert L. Price, PhD Research Professor and Director, Instrumentation Resource Facility School of Medicine, USC Bldg 1 Room B60 6439 Garner's Ferry Road Columbia, SC 29208
I was also looking recently to see what programs are available world wide and had the same difficulty finding such programs. It appears that the MSA provides Certification of Technologist(Scientists) in Biological TEM but I do not believe that this would meet the requirement that the CAP accreditation is referring to.
Here in Australia, this accreditation requirement is meet by our laboratories through enrolment in the Transmission Electron Microscopy Module of the Royal College of Pathologists Australasia Anatomical Pathology Quality Assurance Program. This is proficiency program for laboratories. To quote the website,"Enrolment in this module provides the participant with up to four cases, both technical and diagnostic in the area of Transmission Electron Microscopy, with emphasis on renal pathology." I understand that there are a number of participants from outside Australia.
I have copied this response to the organisers who may be able to forward some further info to you, otherwise go to www.rcpaqapa.netcore.com.au/cgi-bin/site/wrapper.pl?c1=Program. A similar program was running in the UK but I don't think it has continued.
I do not yet know what is available in Europe, perhaps someone in that region could kindly let us know.
Hope this helps, Naomi Pathology Queensland, Australia
} } } {oshel1pe-at-cmich.edu} 12/10/2011 2:21 am } } }
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
Please note that I have already suggested going to the MSA certification website. But: is this sufficient for clinical/pathology certification in TEM?
} Date: Fri, 9 Dec 2011 08:03:56 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: Cathy Kuehner BEMT/MSA {Cathy.Kuehner-at-hcmed.org} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Friday, December 09, } 2011 at 08:03:55 AM. } } realname - Cathy Kuehner BEMT/MSA } Email - Cathy.Kuehner-at-hcmed.org } ORGANIZATION - Hennepin Co Medical Center } LOCATION - Minneapolis, Mn 55404, USA } SUBJECT_OF_QUESTION - Proficiency testing } QUESTION - The clinical/patholgy labs at our institution are } accredited by the College of American Pathologists. } For our next inspection CAP states that all accredited labs must } participate in a proficiency program. } We are having a difficult time finding a proficiency program for } TEM. CAP does not have program for EM. } Do know know how other diagnostic labs deal with this issue?
==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Fri Dec 9 10:13:41 2011 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB9GDfpM003272 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 10:13:41 -0600 4, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 4, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pB9GDbpl020891 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 11:13:40 -0500 4, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Fri, 9 Dec 4, 24 -- 2011 11:13:38 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Message-ID: {a06240808cb07e3b87f50-at-[141.209.160.249]} 4, 24 -- Date: Fri, 9 Dec 2011 11:13:35 -0500 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: clinical programs for EM proficiency 4, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 24 -- X-Originating-IP: [141.209.160.249] 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 4, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 02G74dEX4 - 418320540949 - 20111209 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 18, 28 -- From prvs=1327a144b4=Naomi_McCallum-at-health.qld.gov.au Sun Dec 11 18:38:38 2011 18, 28 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 18, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBC0cZmj022377 18, 28 -- for {Microscopy-at-microscopy.com} ; Sun, 11 Dec 2011 18:38:37 -0600 18, 28 -- Received: from gwd-mail05.remote.health.qld.gov.au (gwd-mail05.remote.health.qld.gov.au [192.168.3.52]) 18, 28 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id pBC0cGEi001171 18, 28 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 10:38:17 +1000 18, 28 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by 18, 28 -- gwd-mail05.remote.health.qld.gov.au (Queensland Health SMTP Server) 18, 28 -- with ESMTP id 18, 28 -- {Ta13e42c14ac0a803341750-at-gwd-mail05.remote.health.qld.gov.au} for 18, 28 -- {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 10:38:16 +1000 18, 28 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au with 18, 28 -- Novell_GroupWise; Mon, 12 Dec 2011 10:38:16 +1000 18, 28 -- Message-Id: {4EE5D994.88AE.00AA.0-at-health.qld.gov.au} 18, 28 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 18, 28 -- Date: Mon, 12 Dec 2011 10:38:13 +1000 18, 28 -- From: "Naomi McCallum" {Naomi_McCallum-at-health.qld.gov.au} 18, 28 -- To: {oshel1pe-at-cmich.edu} 18, 28 -- Cc: {Microscopy-at-microscopy.com} 18, 28 -- Subject: Re: [Microscopy] clinical programs for EM proficiency 18, 28 -- References: {201112091621.pB9GLZ5G014313-at-ns.microscopy.com} 18, 28 -- In-Reply-To: {201112091621.pB9GLZ5G014313-at-ns.microscopy.com} 18, 28 -- Mime-Version: 1.0 18, 28 -- Content-Type: text/plain; charset="us-ascii" 18, 28 -- Content-Disposition: inline 18, 28 -- Content-Transfer-Encoding: 8bit 18, 28 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBC0cZmj022377 ==============================End of - Headers==============================
my stock of glutaraldehyde for electron microscopy has run out and I have to perform fixation for EM in time, however, the glutaraldehyde EM-grade (highest purity) I use is not available from the producer SERVA (SERVA order no 23114; 25% glutaraldehyde) at the moment and the EM-suppliers can't help either.
Can anybody borrow me around 50 ml of unopened SERVA 23114 glutaraldehyde within the next days ? or, if having experience with the procedure, send me his instruction how to distill standard glutaraldehyde to highest purity according to Anderson (2-times-vacuum-double-distilled; active-charcoal-filtered) (Anderson 1967 J Histochem Cytochem)
greetings, Peter Heimann
please answer off-line to peter.heimann-at-uni-bielefeld.de
Hello All, Recently, a client came in to have some pulp fibers embedded in polyvinyl alchol(PVOH) sectioned(~70nm). The ultimate goal is to have the samples viewed using a TEM. The problem, PVOH is soluble in water. Does anyone have experience sectioning PVOH? Are there any liquids I could substitute for water to fill up the trough on my diamond knife? Any help will be greatly appreciated.
-Joe
-- Joseph Heintz Lab/BBPIC Manager University of Wisconsin-Madison Room 1048, Animal Sciences Building 1675 Observatory Dr. Madison, WI 53706 608.263.4162
==============================Original Headers============================== 3, 26 -- From josephheintz-at-gmail.com Mon Dec 12 11:03:56 2011 3, 26 -- Received: from mail-lpp01m010-f41.google.com (mail-lpp01m010-f41.google.com [209.85.215.41]) 3, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCH3tm1010703 3, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:03:56 -0600 3, 26 -- Received: by lahi5 with SMTP id i5so2446257lah.0 3, 26 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 09:03:55 -0800 (PST) 3, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 26 -- d=gmail.com; s=gamma; 3, 26 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 3, 26 -- :subject:to:content-type; 3, 26 -- bh=96TorHBQDftJBY6w3kgAFP0JGyN8H48wJSpi0dVItbs=; 3, 26 -- b=P8yvBYbDoi9R0IqCDw0OCNJsOC8/LSQID5iLuVuflj5ZD1XuoL4DJ2mFM9mVAU47g/ 3, 26 -- 250IxfUn/WJnp0Oo+IAEV8PYGQfVp2H1HHhPM4CEJhtGpTSkF7huA1oqlMBWy1Mp7uS3 3, 26 -- LZu2ZjDQ8CwH7CfTDVczrvYhX5rnzFfCWD5No= 3, 26 -- Received: by 10.152.124.101 with SMTP id mh5mr11433243lab.16.1323709435384; 3, 26 -- Mon, 12 Dec 2011 09:03:55 -0800 (PST) 3, 26 -- MIME-Version: 1.0 3, 26 -- Sender: josephheintz-at-gmail.com 3, 26 -- Received: by 10.152.43.74 with HTTP; Mon, 12 Dec 2011 09:03:34 -0800 (PST) 3, 26 -- From: Joseph Heintz {jheintz-at-wisc.edu} 3, 26 -- Date: Mon, 12 Dec 2011 11:03:34 -0600 3, 26 -- X-Google-Sender-Auth: ClZ0rUpyo97-LIeIacVFGXris5c 3, 26 -- Message-ID: {CALFNmMDwMygb4vdBnD0xFsxvAm7AKkueAHAcjth9Urh=3xkCww-at-mail.gmail.com} 3, 26 -- Subject: Sectioning Water Soluble Material 3, 26 -- To: Microscopy-at-microscopy.com 3, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both wadowska-at-upei.ca as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: wadowska-at-upei.ca Name: Dorota Wadowska
Organization: Atlantic Veterinary College at UPEI
Title-Subject: [Filtered] H7500 OBJ aperture
Message: Hi, I am looking for a supplier of objective lens aperture strip for H 7500. Hitachi sent me a price quote which seems to me too high. We had an alternate source but the quality of a strip was not satisfactory. If you know of a supplier who asks for a reasonable price and the quality is not compromised please let me know. Thank you Dorota Login Host: 137.149.102.148 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Dec 12 11:34:19 2011 7, 25 -- Received: from znl.com ([206.69.208.20]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCHYJOC028219 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:34:19 -0600 7, 25 -- Received: from localhost (localhost [127.0.0.1]) 7, 25 -- by znl.com (Postfix) with ESMTP id 13E04455D1E 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:34:19 -0600 (CST) 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 7, 25 -- Received: from znl.com ([127.0.0.1]) 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 7, 25 -- with ESMTP id 8b89sbUGo7EU for {microscopy-at-microscopy.com} ; 7, 25 -- Mon, 12 Dec 2011 11:34:15 -0600 (CST) 7, 25 -- Received: from surfer-30-1-53.surfnet.iacbox (unknown [81.30.44.42]) 7, 25 -- by znl.com (Postfix) with ESMTPA id 294ED455D0E 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:34:14 -0600 (CST) 7, 25 -- Message-ID: {4EE63B11.1030607-at-microscopy.com} 7, 25 -- Date: Mon, 12 Dec 2011 18:34:09 +0100 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 7, 25 -- Reply-To: wadowska-at-upei.ca 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 7, 25 -- MIME-Version: 1.0 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 25 -- Subject: viaWWW:H7500 OBJ aperture 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 7, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
We have used the obj aperture strips from the third party before. Yes, they were not good in quality (it was possible that we just had back luck). We then purchased from Hitachi a custom-made aperture strip that has three 20-micron and one 50-micron apertures since we usually do not use larger apertures on this microscope anyway. The total cost was still high but we will be able to use all apertures. Hope this helps. Thank you.
Hong
On 12/12/11 12:35 PM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
________________________________
This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.
If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).
==============================Original Headers============================== 10, 39 -- From hyi-at-emory.edu Mon Dec 12 12:05:22 2011 10, 39 -- Received: from ws-mr2.cc.emory.edu (ws-mr2.cc.emory.edu [170.140.50.232]) 10, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCI5MKJ013456 10, 39 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 12:05:22 -0600 10, 39 -- Received: from e14edge2n.Eu.Emory.Edu (emoryfloatdmz.cc.emory.edu [170.140.52.254]) 10, 39 -- by ws-mr2.cc.emory.edu (8.13.8/8.13.1) with ESMTP id pBCI5G3U017384 10, 39 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 13:05:16 -0500 10, 39 -- Received: from E14CH1N.Enterprise.emory.net (10.240.8.113) by 10, 39 -- e14edge2n.Eu.Emory.Edu (170.140.53.162) with Microsoft SMTP Server (TLS) id 10, 39 -- 14.2.247.3; Mon, 12 Dec 2011 13:05:16 -0500 10, 39 -- Received: from E14MBX15N.Enterprise.emory.net ([fe80::fcd7:9c7d:7350:3f64]) by 10, 39 -- e14ch1n.Enterprise.emory.net ([::1]) with mapi id 14.01.0339.001; Mon, 12 Dec 10, 39 -- 2011 13:05:15 -0500 10, 39 -- From: "Yi, Hong" {hyi-at-emory.edu} 10, 39 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 10, 39 -- Subject: Re: [Microscopy] viaWWW:H7500 OBJ aperture 10, 39 -- Thread-Topic: [Microscopy] viaWWW:H7500 OBJ aperture 10, 39 -- Thread-Index: AQHMuPSDukumXq7gPEWpAhbI8mAcCJXYf0eA 10, 39 -- Date: Mon, 12 Dec 2011 18:05:15 +0000 10, 39 -- Message-ID: {CB0BA66F.62C4%hyi-at-emory.edu} 10, 39 -- In-Reply-To: {201112121735.pBCHZoDu031607-at-ns.microscopy.com} 10, 39 -- Accept-Language: en-US 10, 39 -- Content-Language: en-US 10, 39 -- X-MS-Has-Attach: 10, 39 -- X-MS-TNEF-Correlator: 10, 39 -- user-agent: Microsoft-MacOutlook/14.12.0.110505 10, 39 -- x-originating-ip: [170.140.30.131] 10, 39 -- Content-Type: text/plain; charset="us-ascii" 10, 39 -- Content-ID: {43B4409CB0B7964CB2848F2EE22AC37B-at-Enterprise.emory.net} 10, 39 -- MIME-Version: 1.0 10, 39 -- X-Emory-MailScanner-Information: Please contact the ISP for more information 10, 39 -- X-Emory-MailScanner-ID: pBCI5G3U017384 10, 39 -- X-Emory-MailScanner: Found to be clean 10, 39 -- X-Emory-MailScanner-SpamCheck: not spam, SpamAssassin (not cached, 10, 39 -- score=-2.301, required 8, autolearn=disabled, RP_MATCHES_RCVD -2.30) 10, 39 -- X-Emory-MailScanner-From: hyi-at-emory.edu 10, 39 -- X-Spam-Status: No 10, 39 -- Content-Transfer-Encoding: 8bit 10, 39 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBCI5MKJ013456 ==============================End of - Headers==============================
We supply aperture bars, strips, and other consumables for Micrion and FEI FIBs and SEMs, and I would be more then happy to work with you on developing a replacement aperture strip for the H7500.
Feel free to contact me offline if you would like to explore this possibility.
Best Wishes :) Valery Ray ================================= PBS&T, MEO Engineering Co., Inc. 290 Broadway, Suite 298 Methuen, MA 01844 USA Phone: +1-978-296-5063 - leave a message with call-back number US Mobile: +1-978-305-0479 Skype: pbstmeo E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 12/12/2011 12:34 PM, microscopylistserver-noreply-at-microscopy.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both wadowska-at-upei.ca as well as the MIcroscopy } Listserver } --------------------------------------------------------------------------- } } Email: wadowska-at-upei.ca Name: Dorota Wadowska } } Organization: Atlantic Veterinary College at UPEI } } Title-Subject: [Filtered] H7500 OBJ aperture } } Message: Hi, } I am looking for a supplier of objective lens aperture strip for H 7500. } Hitachi sent me a price quote which seems to me too high. We had an } alternate source but the quality of a strip was not satisfactory. If you } know of a supplier who asks for a reasonable price and the quality is } not compromised please let me know. } Thank you Dorota } Login Host: 137.149.102.148 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 7, 25 -- From microscopylistserver-noreply-at-microscopy.com Mon Dec 12 11:34:19 2011 } 7, 25 -- Received: from znl.com ([206.69.208.20]) } 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCHYJOC028219 } 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:34:19 -0600 } 7, 25 -- Received: from localhost (localhost [127.0.0.1]) } 7, 25 -- by znl.com (Postfix) with ESMTP id 13E04455D1E } 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:34:19 -0600 (CST) } 7, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 7, 25 -- Received: from znl.com ([127.0.0.1]) } 7, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 7, 25 -- with ESMTP id 8b89sbUGo7EU for {microscopy-at-microscopy.com} ; } 7, 25 -- Mon, 12 Dec 2011 11:34:15 -0600 (CST) } 7, 25 -- Received: from surfer-30-1-53.surfnet.iacbox (unknown [81.30.44.42]) } 7, 25 -- by znl.com (Postfix) with ESMTPA id 294ED455D0E } 7, 25 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 11:34:14 -0600 (CST) } 7, 25 -- Message-ID: {4EE63B11.1030607-at-microscopy.com} } 7, 25 -- Date: Mon, 12 Dec 2011 18:34:09 +0100 } 7, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 7, 25 -- Reply-To: wadowska-at-upei.ca } 7, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 7, 25 -- MIME-Version: 1.0 } 7, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 25 -- Subject: viaWWW:H7500 OBJ aperture } 7, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 7, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 5, 38 -- From vray-at-partbeamsystech.com Mon Dec 12 12:05:55 2011 5, 38 -- Received: from nm29.access.bullet.mail.mud.yahoo.com (nm29.access.bullet.mail.mud.yahoo.com [66.94.237.94]) 5, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pBCI5taP013982 5, 38 -- for {microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 12:05:55 -0600 5, 38 -- Received: from [66.94.237.196] by nm29.access.bullet.mail.mud.yahoo.com with NNFMP; 12 Dec 2011 18:05:54 -0000 5, 38 -- Received: from [98.138.206.47] by tm7.access.bullet.mail.mud.yahoo.com with NNFMP; 12 Dec 2011 18:05:54 -0000 5, 38 -- Received: from [127.0.0.1] by smtp110.biz.mail.ne1.yahoo.com with NNFMP; 12 Dec 2011 18:05:54 -0000 5, 38 -- X-Yahoo-Newman-Id: 215159.74259.bm-at-smtp110.biz.mail.ne1.yahoo.com 5, 38 -- X-Yahoo-Newman-Property: ymail-3 5, 38 -- X-YMail-OSG: 2fZXOHQVM1lHdc.sHmufyNd_dtmRs4qenx3j8bV3m2JkQlj 5, 38 -- Y1ACD8TTkyEOZ6.GzfklBU37kbj.v0zj2whBC_L0gTG6doccdPE9AQytkOAN 5, 38 -- p3Maj8HnVMyby0YXKX4.eEALgDEOEu9Bz2exXA5zLz11iEoCbjWjc0Av6Y8o 5, 38 -- a.UWNXv7DgW2SHCJ2YzHL5LowUui00GdC7uJ0TfJ8iXBFGUYIqJwSEJzEt7g 5, 38 -- zgSSUwnGyOCj130DmfiVGbx2sdcyj6zyS8G0.sorvkClAxJ7U4H7DEwA43q0 5, 38 -- zpFf.WX9.6PgxRoG_Xh2HuYS2pW0TPXG_L_Hxgdl4uqPb38bt1k92UCw8SqK 5, 38 -- _wjF4l6US6OGfEs6HF5FzZtS_ElD5POeZofo0T.C2lrnarc7sv2h71gWkNbB 5, 38 -- bofo420eQwaxhnZmWJhI4qHTo6Fw387CbgWGsybbRqozyunDR8JmYOUELyra 5, 38 -- NsEkG4ZoEPUTMzHn_LDM5PwNEEzqNyhy4xJgbL_A_UMsgvpTeexnqQp4PeyH 5, 38 -- 0RIvDVxvzEiRx3Ed63BOzMXXIg7Ox6dvTJwmrAulZAexN4oi.KjqJLHWs64y 5, 38 -- eRpLKLJKXEuhxnbDPxehudMzyZtOoRHtBa_ev1Ox_VljqPbi0JP3MSSxD5jD 5, 38 -- LWRi9_mV7SwC2HurTuZmbTd861nfoVIpnKXA7Q3b1tuJbkHVlhQn_n.flcxM 5, 38 -- - 5, 38 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA-- 5, 38 -- Received: from [192.168.1.127] (vray-at-71.174.120.150 with plain) 5, 38 -- by smtp110.biz.mail.ne1.yahoo.com with SMTP; 12 Dec 2011 10:05:53 -0800 PST 5, 38 -- Message-ID: {4EE64282.3060708-at-partbeamsystech.com} 5, 38 -- Date: Mon, 12 Dec 2011 13:05:54 -0500 5, 38 -- From: "vray-at-partbeamsystech.com" {vray-at-partbeamsystech.com} 5, 38 -- Reply-To: vray-at-partbeamsystech.com 5, 38 -- Organization: PBS&T 5, 38 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 5, 38 -- MIME-Version: 1.0 5, 38 -- To: wadowska-at-upei.ca, microscopy-at-microscopy.com 5, 38 -- Subject: Re: [Microscopy] viaWWW:H7500 OBJ aperture 5, 38 -- References: {201112121734.pBCHYwpw029502-at-ns.microscopy.com} 5, 38 -- In-Reply-To: {201112121734.pBCHYwpw029502-at-ns.microscopy.com} 5, 38 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Does anyone know who supplies Hiraoka Staining Kits in the US for staining EM grids? Polysciences and EMS have discontinued selling them.
Thanks,
Carol
-- Carol Cooke Electron Microscope Lab Manager Johns Hopkins University Neurology-Peripheral Nerve Division Pathology-537 600 N. Wolfe Street Baltimore,MD 21287 Lab Phone 410-955-1420 Office Phone 410-955-1442
Carol Cooke Electron Microscope Lab Manager Johns Hopkins University Neurology-Peripheral Nerve Division Pathology-537 600 N. Wolfe Street Baltimore,MD 21287 Lab Phone 410-955-1420 Office Phone 410-955-1442
==============================Original Headers============================== 8, 29 -- From prvs=320c499cc=cooke-at-jhmi.edu Mon Dec 12 14:06:08 2011 8, 29 -- Received: from ipex4.johnshopkins.edu (ipex4.johnshopkins.edu [128.220.161.141]) 8, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCK68YE016912 8, 29 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 14:06:08 -0600 8, 29 -- X-IronPort-AV: E=Sophos;i="4.71,340,1320642000"; 8, 29 -- d="scan'208";a="123514670" 8, 29 -- Received: from commsmail.johnshopkins.edu ([10.173.7.15]) 8, 29 -- by ipex4.johnshopkins.edu with ESMTP/TLS/RC4-MD5; 12 Dec 2011 15:06:07 -0500 8, 29 -- MIME-version: 1.0 8, 29 -- Content-transfer-encoding: 7BIT 8, 29 -- Content-disposition: inline 8, 29 -- Content-type: text/plain; CHARSET=US-ASCII 8, 29 -- Received: from johnshopkins.edu ([unknown] [10.173.7.11]) 8, 29 -- by commsmail.johnshopkins.edu 8, 29 -- (Sun Java(tm) System Messaging Server 7u3-12.01 64bit (built Oct 15 2009)) 8, 29 -- with ESMTP id {0LW3008V4X66AF00-at-commsmail.johnshopkins.edu} for 8, 29 -- Microscopy-at-microscopy.com; Mon, 12 Dec 2011 15:06:06 -0500 (EST) 8, 29 -- Received: from [10.173.109.13] (Forwarded-For: [10.17.104.87]) 8, 29 -- by commsmail.johnshopkins.edu (mshttpd); Mon, 12 Dec 2011 15:06:06 -0500 8, 29 -- From: Carol A Cooke {cooke-at-jhmi.edu} 8, 29 -- To: Microscopy-at-microscopy.com 8, 29 -- Message-id: {7250c7ca5551.4ee6185e-at-johnshopkins.edu} 8, 29 -- Date: Mon, 12 Dec 2011 15:06:06 -0500 8, 29 -- X-Mailer: Sun Java(tm) System Messenger Express 7u3-12.01 64bit (built Oct 15 8, 29 -- 2009) 8, 29 -- Content-language: en 8, 29 -- Subject: Hiraoka Staining Kits 8, 29 -- X-Accept-Language: en 8, 29 -- Priority: normal ==============================End of - Headers==============================
Lately I've noticed a very thin film of clear, oily residue floating on top of the water reservoir in my Haskris chiller that services the SEM and at times the vacuum evaporator. It returns a few days after changing the water. Everything else seems to be OK - flow, temperature, noises, cycling, etc. The sight glass on the refrigeration system is clear - no bubbles or condensation.
For several years I ran the system with 10% ethylene glycol as recommended by Haskris. I got tired of disposing of this so I switched to bleach a few years ago and haven't noticed this residue until recently. I'm wondering if the residue isn't simply residual glycol leaching out of the water lines? I find it strange that it would appear so long after switching away from glycol...
Any past experience with such issues would be greatly appreciated.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Bozone (n.): The substance surrounding stupid people that stops bright ideas from penetrating. The bozone layer, unfortunately, shows little sign of breaking down in the near future.
==============================Original Headers============================== 11, 19 -- From jehrman-at-mta.ca Mon Dec 12 14:17:49 2011 11, 19 -- Received: from smtpx.mta.ca (smtpx.mta.ca [138.73.1.4]) 11, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCKHkpJ031639 11, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 14:17:48 -0600 11, 19 -- Received: from host-22-169.mta.ca ([138.73.22.169]:51308) 11, 19 -- by smtpx.mta.ca with esmtpsa (TLSv1:AES256-SHA:256) 11, 19 -- (Exim 4.76) 11, 19 -- (envelope-from {jehrman-at-mta.ca} ) 11, 19 -- id 1RaCJl-0001VU-Ta 11, 19 -- for Microscopy-at-microscopy.com; Mon, 12 Dec 2011 16:17:45 -0400 11, 19 -- Message-ID: {4EE66169.2050506-at-mta.ca} 11, 19 -- Date: Mon, 12 Dec 2011 16:17:45 -0400 11, 19 -- From: "James M. Ehrman" {jehrman-at-mta.ca} 11, 19 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:8.0) Gecko/20111105 Thunderbird/8.0 11, 19 -- MIME-Version: 1.0 11, 19 -- To: Microscopy Listserv {Microscopy-at-microscopy.com} 11, 19 -- Subject: oily residue in chiller tank 11, 19 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 11, 19 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Hi Folks - I believe that the RCPA TEM QAP is the only external program there is for diagnostic TEM – diagnostic EM is one of the anatomical pathology modules. TRANSMISSION ELECTRON MICROSCOPY Enrolment in this module provides the participant with up to four cases, both technical and diagnostic in the area of Transmission Electron Microscopy, with emphasis on renal pathology. One technical case is a request for a submission of electron micrographs of a specified clinical nature. Assessment results and reports of results containing an analysis of participants' responses are made available on the website and sent to participants after each survey.
ACEM in the UK used to run a program for members but this was run by volunteers and I don’t think that this would count as far as accreditation is concerned – I don’t know if this is still running – see the ACEM web page for contacts:
http://www.acem.org.uk/
There is a Veterans Affairs EM program in the US but I presume that this is only open to units that service the VHA. See:
http://www.va.gov/DIAGNOSTICEM/index.asp
VHA National EM Program Contacts
Contact Information Telephone: 919-286-6925 or 919-286-0411 ext. 6648 or ext. 5218
FAX: 919-286-6818 Postal address Diagnostic Electron Microscopy Program John D. Shelburne, M.D., Ph.D. P&LMS (113) 508 Fulton Street Durham, NC 27705
Electronic mail General Information: Edoris.LeFurgey-at-va.gov Webmaster: Edoris.LeFurgey-at-va.gov E-Mail to: John Shelburne-at-va.gov
I think there have also been programs for speciality areas like virology (eg. the UK in the mid 1980s) and particulates (asbestos etc) but again these don’t really apply to diagnostics and I don’t know if any of them are currently active.
I am the convenor for the RCPA program and it is open to all – new participants are welcome. We currently have ~26 labs enrolled in SE Asia and Europe. Please contact Erin Little or the RCPA QAP office through the website for details of fees etc. If US labs have specific needs then we could discuss these and possibly tailor the program to suit – we are always open to new ideas for content etc so suggestions are welcome.
Best wishes – John
CC Erin Little RCPA
John W Stirling BSc(Hons), MLett, AFRCPA, M-AIMS, FRMS
Head of Unit - The Centre for Ultrastructural Pathology SA Pathology
E john.stirling-at-health.sa.gov.au T 61-8-8222 3941 (office) T 61-8-8222 3202 (laboratory) F 61-8-8222 3204 (Surgical Pathology)
www.sapathology.sa.gov.au
The Centre for Ultrastructural Pathology Surgical Pathology - SA Pathology (RAH) Frome Road PO Box 14 Rundle Mall Adelaide SA 5000 AUSTRALIA
Quality Pathology supporting Training and Research
This email may contain confidential information, which also may be legally privileged. Only the intended recipient(s) may access, use, distribute or copy this e-mail. If this e-mail is received in error, please inform the sender by return e-mail and delete the original. If there are doubts about the validity of this message, please contact the sender by telephone. It is the recipient's responsibility to check the e-mail and any attached files for viruses.
-----Original Message----- X-from: Naomi McCallum [mailto:Naomi_McCallum-at-health.qld.gov.au] Sent: Monday, 12 December 2011 11:08 To: oshel1pe-at-cmich.edu Cc: Microscopy-at-microscopy.com
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. ****************************************************************************************
Please note that I have already suggested going to the MSA certification website. But: is this sufficient for clinical/pathology certification in TEM?
} Date: Fri, 9 Dec 2011 08:03:56 -0800 } From: {AssociationManagement-at-microscopy.org} } Reply-To: Cathy Kuehner BEMT/MSA {Cathy.Kuehner-at-hcmed.org} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Friday, December 09, } 2011 at 08:03:55 AM. } } realname - Cathy Kuehner BEMT/MSA } Email - Cathy.Kuehner-at-hcmed.org } ORGANIZATION - Hennepin Co Medical Center } LOCATION - Minneapolis, Mn 55404, USA } SUBJECT_OF_QUESTION - Proficiency testing } QUESTION - The clinical/patholgy labs at our institution are } accredited by the College of American Pathologists. } For our next inspection CAP states that all accredited labs must } participate in a proficiency program. } We are having a difficult time finding a proficiency program for } TEM. CAP does not have program for EM. } Do know know how other diagnostic labs deal with this issue?
==============================Original Headers============================== 4, 24 -- From oshel1pe-at-cmich.edu Fri Dec 9 10:13:41 2011 4, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 4, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB9GDfpM003272 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 10:13:41 -0600 4, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 4, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pB9GDbpl020891 4, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 11:13:40 -0500 4, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 4, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Fri, 9 Dec 4, 24 -- 2011 11:13:38 -0500 4, 24 -- MIME-Version: 1.0 4, 24 -- Message-ID: {a06240808cb07e3b87f50-at-[141.209.160.249]} 4, 24 -- Date: Fri, 9 Dec 2011 11:13:35 -0500 4, 24 -- To: {Microscopy-at-microscopy.com} 4, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 4, 24 -- Subject: clinical programs for EM proficiency 4, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 4, 24 -- X-Originating-IP: [141.209.160.249] 4, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 4, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 4, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 4, 24 -- X-CanItPRO-Stream: default 4, 24 -- X-Canit-Stats-ID: 02G74dEX4 - 418320540949 - 20111209 4, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **********************************************************************************
==============================Original Headers============================== 7, 25 -- From prvs=1327a144b4=Naomi_McCallum-at-health.qld.gov.au Mon Dec 12 17:34:01 2011 7, 25 -- Received: from gwd-mailedge05.health.qld.gov.au (smtp3.health.qld.gov.au [165.86.81.114]) 7, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBCNXxTS022950 7, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 12 Dec 2011 17:34:00 -0600 7, 25 -- Received: from gwd-mail04.remote.health.qld.gov.au (gwd-mail04.remote.health.qld.gov.au [192.168.3.51]) 7, 25 -- by gwd-mailedge05.health.qld.gov.au (8.14.1/8.14.1) with ESMTP id pBCNXhNa007246 7, 25 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 09:33:44 +1000 7, 25 -- Received: from health-cs11.health.qld.gov.au (unverified [10.17.112.31]) by gwd-mail04.remote.health.qld.gov.au 7, 25 -- (Queensland Health SMTP Server) with ESMTP id {Ta1432df8a9c0a80333378-at-gwd-mail04.remote.health.qld.gov.au} for {Microscopy-at-microscopy.com} ; 7, 25 -- Tue, 13 Dec 2011 09:33:40 +1000 7, 25 -- Received: from CORPORATE-GWIA01-MTA by health-cs11.health.qld.gov.au 7, 25 -- with Novell_GroupWise; Tue, 13 Dec 2011 09:33:40 +1000 7, 25 -- Message-Id: {4EE71BEE.88AE.00AA.0-at-health.qld.gov.au} 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 8.0.2 7, 25 -- Date: Tue, 13 Dec 2011 09:33:36 +1000 7, 25 -- From: "Naomi McCallum" {Naomi_McCallum-at-health.qld.gov.au} 7, 25 -- To: {Microscopy-at-microscopy.com} 7, 25 -- Subject: Fwd: RE: [Microscopy] clinical programs for EM proficiency 7, 25 -- References: {201112091621.pB9GLZ5G014313-at-ns.microscopy.com} 7, 25 -- {4EE5D994.88AE.00AA.0-at-health.qld.gov.au} 7, 25 -- {6ACF69B4639A8E4996E940826AF28BE0E1E4C34C97-at-EMSCM007.sagemsmrd01.sa.gov.au} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=UTF-8 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- Content-Disposition: inline ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tchou-at-stevens.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tchou-at-stevens.edu Name: Alex Chou
Organization: Stevens Tech
Title-Subject: [Filtered] UA stain on DNA nanoparticles for TEM imaging
Message: We are trying to image DNA nanoparticles in TEM via UA staining. It turns out the DNA particles aggregated heavily via the UA solution I made out of the regular DI water. According to my collaborator, the UA solution I made has PH ~ 3 which causes the nano-particles stick together. We tried to adjust the PH, but UA solution starts to show sign of precipitation sooner it reaches to PH ~6. But, we really need something that has PH around 7 to 8! So, the questions are:
1. is there a way to adjust UA solution to PH around 7 to 8?
2. or, is there another stain solution that has PH ~ 7 to 8 we can used for imaging DNA structures?
Hello All, I've had a request to share a summary of the responses I have received, so here are two responses that sum up all the responses I have gathered:
- Our go to for that kind of sectioning would normally be a dry-Cryo technique.You can try sectioning that material with polyethylene glycol in your boat, although I suspect that PVA is also solvable in PEG. PEG is also a nasty bit of business for your diamonds, so I wouldn't use your best.
-Any chance you could have access to an ultramicrotome with a cryo attachment? You may have to play with the temperature, but it would basically be like dry-sectioning OCT (which is PVA + ethylene glycol + "nonreactive ingredients")in a regular cryostat.
Thanks to all who supplied suggestions!
-Joe
-- Joseph Heintz Lab/BBPIC Manager University of Wisconsin-Madison Room 1048, Animal Sciences Building 1675 Observatory Dr. Madison, WI 53706 608.263.4162
==============================Original Headers============================== 6, 26 -- From josephheintz-at-gmail.com Tue Dec 13 10:24:26 2011 6, 26 -- Received: from mail-ww0-f49.google.com (mail-ww0-f49.google.com [74.125.82.49]) 6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDGONWg020734 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:24:23 -0600 6, 26 -- Received: by wgbdt11 with SMTP id dt11so9631435wgb.18 6, 26 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 08:24:22 -0800 (PST) 6, 26 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 26 -- d=gmail.com; s=gamma; 6, 26 -- h=mime-version:sender:from:date:x-google-sender-auth:message-id 6, 26 -- :subject:to:content-type; 6, 26 -- bh=wd1Zb2FitSGkZu9n33iD9v4bzagJEpGhxL949mkecdM=; 6, 26 -- b=HsyHYXhD5+w/BvXm4ilO+kM9pCfyW6lW5RxL4dLrC1cD/OfTS+SIkRd8uTxUZp7yyD 6, 26 -- 0t+KJ/fnHiOKEF7mlLgI3zcfwOZfY7CShjVnykbPBSBJzza9QcpPHwltDMGTVto71JB9 6, 26 -- eSaSP92q0L084JWpHH34+0kZXPXZZZ802wbu4= 6, 26 -- Received: by 10.227.206.10 with SMTP id fs10mr157261wbb.13.1323793462134; Tue, 6, 26 -- 13 Dec 2011 08:24:22 -0800 (PST) 6, 26 -- MIME-Version: 1.0 6, 26 -- Sender: josephheintz-at-gmail.com 6, 26 -- Received: by 10.180.86.233 with HTTP; Tue, 13 Dec 2011 08:24:01 -0800 (PST) 6, 26 -- From: Joseph Heintz {jheintz-at-wisc.edu} 6, 26 -- Date: Tue, 13 Dec 2011 10:24:01 -0600 6, 26 -- X-Google-Sender-Auth: Lfz1oDSdcZWjZKGxt0aWzjMxq-M 6, 26 -- Message-ID: {CALFNmMBojT-jJuoQGQKD6x+1qKvpHzu2-q031tf0zQ838O=ULA-at-mail.gmail.com} 6, 26 -- Subject: Sectioning Water Soluble Material Summary 6, 26 -- To: Microscopy-at-microscopy.com 6, 26 -- Content-Type: text/plain; charset=ISO-8859-1 ==============================End of - Headers==============================
Do you need to positively stain the DNA, or are you doing a negative stain? If a negative stain, try phosphotungstic acid - that can be adjusted to pH 8.
Phil
} Email: Name: Alex Chou } } Organization: Stevens Tech } } Title-Subject: [Filtered] UA stain on DNA nanoparticles for TEM imaging } } Message: We are trying to image DNA nanoparticles in TEM via UA } staining. It turns out the DNA particles aggregated heavily via the UA } solution I made out of the regular DI water. According to my } collaborator, the UA solution I made has PH ~ 3 which causes the } nano-particles stick together. We tried to adjust the PH, but UA } solution starts to show sign of precipitation sooner it reaches to PH } ~6. But, we really need something that has PH around 7 to 8! So, the } questions are: } } 1. is there a way to adjust UA solution to PH around 7 to 8? } } 2. or, is there another stain solution that has PH ~ 7 to 8 we can used } for imaging DNA structures? } } Thanks, } } Alex
-- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576
==============================Original Headers============================== 5, 28 -- From oshel1pe-at-cmich.edu Tue Dec 13 10:28:04 2011 5, 28 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDGS329029909 5, 28 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:28:04 -0600 5, 28 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 5, 28 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pBDGS0Lk013151; 5, 28 -- Tue, 13 Dec 2011 11:28:01 -0500 5, 28 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 5, 28 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 13 Dec 5, 28 -- 2011 11:28:00 -0500 5, 28 -- MIME-Version: 1.0 5, 28 -- Message-ID: {a06240810cb0d2d342d73-at-[141.209.160.249]} 5, 28 -- In-Reply-To: {201112131625.pBDGP9nx021324-at-ns.microscopy.com} 5, 28 -- References: {201112131625.pBDGP9nx021324-at-ns.microscopy.com} 5, 28 -- Date: Tue, 13 Dec 2011 11:27:58 -0500 5, 28 -- To: {tchou-at-stevens.edu} 5, 28 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 5, 28 -- Subject: Re: [Microscopy] viaWWW:UA stain on DNA nanoparticles for TEM 5, 28 -- imaging 5, 28 -- CC: {Microscopy-at-microscopy.com} 5, 28 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 5, 28 -- X-Originating-IP: [141.209.160.249] 5, 28 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 5, 28 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 5, 28 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 5, 28 -- X-CanItPRO-Stream: default 5, 28 -- X-Canit-Stats-ID: 02G8Es0qE - 51263408f50d - 20111213 5, 28 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
This should fit in with the current thread about microscopes for children. Please note that it is for a specifc recommendation of brand for an elementary school.
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } Date: Tue, 13 Dec 2011 08:58:19 -0800 } Reply-To: Jennifer Buchanan {jen.buchanan-at-dmr.ms.gov} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Tuesday, December 13, } 2011 at 08:58:18 AM. } } realname - Jennifer Buchanan } Email - jen.buchanan-at-dmr.ms.gov } ORGANIZATION - Grand Bay National Estuarine Research Reserve } EDUCATION - K-8 Grade Grammar School } QUESTION - I just read on this site that a monocular scope is better } for younger children. I have a small grant to buy some microscopes } for a local elementary school that is setting up a science lab. I } would like a recommendation for a monocular dissecting scope with } LED and rechargeable batteries. Any suggestions? } --
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Tue Dec 13 11:06:25 2011 2, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDH6PrN002582 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 11:06:25 -0600 2, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 2, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pBDH6OuJ015699 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 12:06:24 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 13 Dec 2, 24 -- 2011 12:06:24 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240816cb0d35f83b75-at-[141.209.160.249]} 2, 24 -- Date: Tue, 13 Dec 2011 12:06:22 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: Monocular dissecting 'scope for elementary school 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 02G8F6oCO - b98066141cc2 - 20111213 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
While I do not challenge the idea that for general use, a monocular microscope is better for youngsters. Most binocular scopes are designed for those of us with 'fatter' heads with larger interpupliary measurements. A dissecting scope, however, by virtue of its functional appellation is designed to provide a holistic image to the operator in order to facilitate 'dissection'[pronounced: 'dis---section' NOT "di(bi)---section," please].
Since most children do not yet have 'fat' heads, it might be more productive for those lessons requiring low to moderate magnification for students to be introduced to 'microscopy' with simple lenses and loupes of various powers up to 20x. In this manner, the users will be optically relaxed during exercises when using magnifiers. One does not have to use 'monster' mags to teach the concepts of 'simple' microscopy. For dissections, one should be able to use an illuminated magnifier on a stand or goose-neck to enhance the dissection process for small specimens whether plant or animal or fossil.
While a student in middle school may be excited to be in the presence of a microscope with magnification between 100-1000x, the learning comes from simple exercises with grampa's magnifier and a newspaper image. "I see dots, Grampa!"
When teaching microscopy, one must remember to mention the preparation of the specimen. E.g., "Class! That slide has two pieces of liver on it. One from an elephant and the other from a mouse. Can you distinguish one from the other? Does one have really big cells and the other really tiny ones? Ah, you noticed!!! One of the two sections has a smooth 'microscopic' boundary while the other does not, and you conclude that the former is from the mouse. Wonderful!!!! Will you bet your lunch on that?"
Happy/Merry Holidays/Christmas,
Fred Monson
Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) Schmucker Science Center South West Chester University, West Chester, PA 19383 610-738-0437
Home Page: http://cmirt.wcupa.edu Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl
If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state. 1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008. 2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: Tuesday, December 13, 2011 12:13 PM To: Monson, Frederick
This should fit in with the current thread about microscopes for children. Please note that it is for a specifc recommendation of brand for an elementary school.
*************************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************************** } Date: Tue, 13 Dec 2011 08:58:19 -0800 } Reply-To: Jennifer Buchanan {jen.buchanan-at-dmr.ms.gov} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Tuesday, December 13, } 2011 at 08:58:18 AM. } } realname - Jennifer Buchanan } Email - jen.buchanan-at-dmr.ms.gov } ORGANIZATION - Grand Bay National Estuarine Research Reserve } EDUCATION - K-8 Grade Grammar School } QUESTION - I just read on this site that a monocular scope is better } for younger children. I have a small grant to buy some microscopes } for a local elementary school that is setting up a science lab. I } would like a recommendation for a monocular dissecting scope with } LED and rechargeable batteries. Any suggestions? } --
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Tue Dec 13 11:06:25 2011 2, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDH6PrN002582 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 11:06:25 -0600 2, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 2, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pBDH6OuJ015699 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 12:06:24 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 13 Dec 2, 24 -- 2011 12:06:24 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240816cb0d35f83b75-at-[141.209.160.249]} 2, 24 -- Date: Tue, 13 Dec 2011 12:06:22 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: Monocular dissecting 'scope for elementary school 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 02G8F6oCO - b98066141cc2 - 20111213 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 18, 37 -- From FMonson-at-wcupa.edu Tue Dec 13 14:11:45 2011 18, 37 -- Received: from MX02.WCUPA.EDU (mx02.wcupa.edu [144.26.129.250]) 18, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDKBigZ023080 18, 37 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 14:11:45 -0600 18, 37 -- X-ASG-Debug-ID: 1323807103-0378c37883194c70001-4CH8be 18, 37 -- Received: from WCU-XCH-02.PASSHE.LCL (wcu-xch-02.wcupa.edu [144.26.0.86]) by MX02.WCUPA.EDU with ESMTP id KiQeLXOrfXtNyhSq (version=TLSv1 cipher=AES128-SHA bits=128 verify=NO); Tue, 13 Dec 2011 15:11:43 -0500 (EST) 18, 37 -- X-Barracuda-Envelope-From: FMonson-at-wcupa.edu 18, 37 -- X-Barracuda-Apparent-Source-IP: 144.26.0.86 18, 37 -- X-ASG-Whitelist: Client 18, 37 -- Received: from WCU-XCH-03.PASSHE.LCL ([fe80::a594:2bd9:809a:5fb1]) by 18, 37 -- WCU-XCH-02.PASSHE.LCL ([fe80::7c95:2a81:a8ef:432b%16]) with mapi id 18, 37 -- 14.01.0270.001; Tue, 13 Dec 2011 15:11:43 -0500 18, 37 -- From: "Monson, Frederick" {FMonson-at-wcupa.edu} 18, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 18, 37 -- CC: "oshel1pe-at-cmich.edu" {oshel1pe-at-cmich.edu} 18, 37 -- Subject: RE: [Microscopy] Monocular dissecting 'scope for elementary school 18, 37 -- Thread-Topic: [Microscopy] Monocular dissecting 'scope for elementary school 18, 37 -- X-ASG-Orig-Subj: RE: [Microscopy] Monocular dissecting 'scope for elementary school 18, 37 -- Thread-Index: AQHMubpsgFX4Yjm9jEm5uCTLGYGS3ZXaK9OQ 18, 37 -- Date: Tue, 13 Dec 2011 20:11:43 +0000 18, 37 -- Message-ID: {899EAA40AE6E47488CE0637489EFB30C10E4EF-at-WCU-XCH-03.PASSHE.LCL} 18, 37 -- References: {201112131712.pBDHCdhY014722-at-ns.microscopy.com} 18, 37 -- In-Reply-To: {201112131712.pBDHCdhY014722-at-ns.microscopy.com} 18, 37 -- Accept-Language: en-US 18, 37 -- Content-Language: en-US 18, 37 -- X-MS-Has-Attach: 18, 37 -- X-MS-TNEF-Correlator: 18, 37 -- x-originating-ip: [10.28.57.79] 18, 37 -- Content-Type: text/plain; charset="us-ascii" 18, 37 -- MIME-Version: 1.0 18, 37 -- X-Barracuda-Connect: wcu-xch-02.wcupa.edu[144.26.0.86] 18, 37 -- X-Barracuda-Start-Time: 1323807103 18, 37 -- X-Barracuda-Encrypted: AES128-SHA 18, 37 -- X-Barracuda-URL: http://SPAMCONTROL.WCUPA.EDU:80/cgi-mod/mark.cgi 18, 37 -- X-Virus-Scanned: by bsmtpd at WCUPA.EDU 18, 37 -- Content-Transfer-Encoding: 8bit 18, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBDKBigZ023080 ==============================End of - Headers==============================
The Setting: Our biological imaging core has a 13 year old JEOL 1230 TEM microscope that has been down a substantial amount of time in the last 2 years. I understand that older versions of TEMs can last much longer than 13 years. However, this instrument was designed in the midst of the analog and digital transition a "tweener". Apparently the "digital wrapper" is antiquated despite the column being reasonably modern. When it breaks, it is difficult to fix. Despite getting our money's worth on the service contract the extended down time meant we didn't bill for the system.
The Questions: Has anyone else encountered this tweener syndrome. Should we be considering replacing the instrument or hoping for the best? Some feel it is almost all new given the number of parts replaced in the last 2 years.
Could a refurbished system avoiding the digital wrapper issues be a solution?
Can a FEI T-20 operating at 200Kv serve as the sole Biological TEM imaging resource? We have access but the instrument is more complicated, our samples require special preparation on that system and low magnification images are harder to acquire.
Are there university or private labs that image biological TEM samples for off-campus users?
I appreciate any input Thanks Mary
Mary Raven Microscopy Facility Director Neuroscience Research Institute& Molecular, Cellular& Developmental Biology Biology 2 Building, room 5173 The University of California Santa Barbara, CA 93106-5060
==============================Original Headers============================== 9, 18 -- From mary.raven-at-lifesci.ucsb.edu Tue Dec 13 18:30:03 2011 9, 18 -- Received: from mailsvr.lifesci.ucsb.edu (mailsvr.lifesci.ucsb.edu [128.111.90.44]) 9, 18 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBE0U2mR011332 9, 18 -- for {Microscopy-at-Microscopy.Com} ; Tue, 13 Dec 2011 18:30:03 -0600 9, 18 -- MIME-version: 1.0 9, 18 -- Content-transfer-encoding: 7BIT 9, 18 -- Content-type: text/plain; CHARSET=US-ASCII; format=flowed 9, 18 -- Received: from [128.111.209.214] (zeus.nri.ucsb.edu [128.111.209.214]) 9, 18 -- by mailsvr.lifesci.ucsb.edu 9, 18 -- (Sun Java(tm) System Messaging Server 7.3-11.01 64bit (built Sep 1 2009)) 9, 18 -- with ESMTPSA id {0LW6001T6421GG00-at-mailsvr.lifesci.ucsb.edu} for 9, 18 -- Microscopy-at-Microscopy.Com; Tue, 13 Dec 2011 16:30:02 -0800 (PST) 9, 18 -- Message-id: {4EE7EE06.4090101-at-lifesci.ucsb.edu} 9, 18 -- Date: Tue, 13 Dec 2011 16:29:58 -0800 9, 18 -- From: Mary Raven {mary.raven-at-lifesci.ucsb.edu} 9, 18 -- User-Agent: Mozilla/5.0 (Windows NT 5.1; rv:8.0) Gecko/20111105 Thunderbird/8.0 9, 18 -- To: Microscopy-at-Microscopy.Com 9, 18 -- Subject: TEM: Input on Options for a Biological Core Facility ==============================End of - Headers==============================
UA precipitates at pH} 6, that is right. But that doesn't mean that no UA remained in the solution, it is just saturated! So the first thing I would do is to filter the UA solution at pH7 (or centrifuge it) and see if it stains. I would bet it does. I also read that 1% lead perchlorate also stains DNA. It may also precipitate at pH7 but see my first remark. Another solution would consist in staining at low pH (and thus having aggregation) and then wash and try different techniques to isolate the nanoparticles, like using salt (neutralize electrostatic charges) or sonicate and so on.
Best regards. Stephane
} Email: Name: Alex Chou } } Organization: Stevens Tech } } Title-Subject: [Filtered] UA stain on DNA nanoparticles for TEM imaging } } Message: We are trying to image DNA nanoparticles in TEM via UA } staining. It turns out the DNA particles aggregated heavily via the UA } solution I made out of the regular DI water. According to my } collaborator, the UA solution I made has PH ~ 3 which causes the } nano-particles stick together. We tried to adjust the PH, but UA } solution starts to show sign of precipitation sooner it reaches to PH } ~6. But, we really need something that has PH around 7 to 8! So, the } questions are: } } 1. is there a way to adjust UA solution to PH around 7 to 8? } } 2. or, is there another stain solution that has PH ~ 7 to 8 we can used } for imaging DNA structures? } } Thanks, } } Alex
==============================Original Headers============================== 5, 38 -- From nizets2-at-yahoo.com Wed Dec 14 07:43:51 2011 5, 38 -- Received: from nm28-vm2.bullet.mail.ne1.yahoo.com (nm28-vm2.bullet.mail.ne1.yahoo.com [98.138.91.128]) 5, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pBEDhoGp008974 5, 38 -- for {microscopy-at-microscopy.com} ; Wed, 14 Dec 2011 07:43:51 -0600 5, 38 -- Received: from [98.138.90.56] by nm28.bullet.mail.ne1.yahoo.com with NNFMP; 14 Dec 2011 13:41:01 -0000 5, 38 -- Received: from [98.138.86.157] by tm9.bullet.mail.ne1.yahoo.com with NNFMP; 14 Dec 2011 13:41:01 -0000 5, 38 -- Received: from [127.0.0.1] by omp1015.mail.ne1.yahoo.com with NNFMP; 14 Dec 2011 13:41:01 -0000 5, 38 -- X-Yahoo-Newman-Property: ymail-3 5, 38 -- X-Yahoo-Newman-Id: 826989.81070.bm-at-omp1015.mail.ne1.yahoo.com 5, 38 -- Received: (qmail 30216 invoked by uid 60001); 14 Dec 2011 13:41:00 -0000 5, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1323870060; bh=M6nJcbIZ4H4MXUgOJLu5RY+ky9sgFhHfY1cOGbwH8kY=; h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=0nuOaIZ6Yspg1EdLVSznNVk4td10iSTjukJHRJOjQAAykF1vk0m7xek7rytIC+Q9a1Fn+hK32FDA2LSd/eYNwnbiMPgOz7OU1jAI3KrAWL85Rh3ftvxQ8v8KdxDhP3apAW2XRt/sG1XfxYPDJqu/HHcvR4cfyet9zXfgCy+dwW8= 5, 38 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 5, 38 -- s=s1024; d=yahoo.com; 5, 38 -- h=X-YMail-OSG:Received:X-Mailer:References:Message-ID:Date:From:Reply-To:Subject:To:In-Reply-To:MIME-Version:Content-Type:Content-Transfer-Encoding; 5, 38 -- b=r4OOwaCDkUu/QcEkmWHm5pgUxbxz6vQVhWp3sAqwkXV9Di26252xgAUVr4KsWH8Hj5YWhAv3xCZJ/ONaYxnPR2SY2iOGEbEII5WZiATxwDhYyyhmUWFQQrSFdaOTyTroQ2S5w095LezndpEtn0i4UUGYuWGONkvM6CcdgaQQnIw=; 5, 38 -- X-YMail-OSG: okgDiX0VM1lUApe9gH9mxuAdaGOshE6LqlKTqbYp_2Bi6ja 5, 38 -- aaJ86FYWm.h9YNy20riAMSZ0uPRZ4Eq02WL1T4eCPUC2imnHlHJO4AmC8bJH 5, 38 -- LnoIYrQY.D3HsZuefLij5ygmv87g9Es49JQ4kbkhMdfbiXQWWJw0uOVSzNWb 5, 38 -- GoXRPx.pX3Qgiyu6MPp_fezQGpJZS5qD3wP96XumNU16lMzgSJYOAKFe5_5C 5, 38 -- UNPJHyi77JHVoZulsjWaqfH3xYojhvhIqKY3n2rY0rK7wjSYttfOtx1wiEZk 5, 38 -- BCkLbETOQxJcJNsgtt5K1bkZfpsGFpu7rhoIASExlJ2jE1zBu2dECWoYIXaz 5, 38 -- OfYLDoQJegW9OXkxDaoBLP_pxVQqNCexCRcObF_JNyVjjKYN1IMXo4.EL7I0 5, 38 -- MN.fLugzp2zcHjLj9kWlvVjmx3lQoI7Bl3CO58ea2nszyJFtA 5, 38 -- Received: from [80.122.101.100] by web110806.mail.gq1.yahoo.com via HTTP; Wed, 14 Dec 2011 05:41:00 PST 5, 38 -- X-Mailer: YahooMailWebService/0.8.115.331698 5, 38 -- References: {201112131630.pBDGUmxZ007168-at-ns.microscopy.com} 5, 38 -- Message-ID: {1323870060.29365.YahooMailNeo-at-web110806.mail.gq1.yahoo.com} 5, 38 -- Date: Wed, 14 Dec 2011 05:41:00 -0800 (PST) 5, 38 -- From: Stephane Nizet {nizets2-at-yahoo.com} 5, 38 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 5, 38 -- Subject: Re: [Microscopy] Re: viaWWW:UA stain on DNA nanoparticles for TEM 5, 38 -- To: "tchou-at-stevens.edu" {tchou-at-stevens.edu} , 5, 38 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 5, 38 -- In-Reply-To: {201112131630.pBDGUmxZ007168-at-ns.microscopy.com} 5, 38 -- MIME-Version: 1.0 5, 38 -- Content-Type: text/plain; charset=iso-8859-1 5, 38 -- Content-Transfer-Encoding: 8bit 5, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBEDhoGp008974 ==============================End of - Headers==============================
From caromel_mrs.isabella-at-yahoo.com.hk Wed Dec 14 23:06:13 2011 Return-Path: {caromel_mrs.isabella-at-yahoo.com.hk} Received: from mail.netrip.mobi (s100.GosakaFL6.vectant.ne.jp [202.215.111.100]) by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBF56CLt014170; Wed, 14 Dec 2011 23:06:13 -0600 Received: from User (unknown [46.183.217.253]) by mail.netrip.mobi (Postfix) with ESMTP id C65B54881DF0; Mon, 12 Dec 2011 16:48:27 +0900 (JST) Reply-To: {caromel_m-at-yahoo.com.hk}
Hi Jennifer,
Following on from Fred's useful advice, and regarding specific microscopes, in the UK I would say visit the websites of the national suppliers to British schools - they know their microscopes, the school market [low prices], and their user base [the kids]. Professional microscopists just wouldn't tolerate the kit schools use and so often can't offer much useful up-to-date advice on particular budget microscope models. In the UK schools national suppliers would be the likes of:
http://www.gxoptical.com/html/education.html
http://www.brunelmicroscopes.co.uk http://www.educationalmicroscopes.co.uk/slides.html [The DM1/DM5 microscopes and a selection of magnifying glasses]
I've found their help lines very useful for general advice of this sort - although I've used them for teenage secondary school-kids who want decent microscopes at home, they will be clued up to Primary/Elementary school needs [although they will probably want to sell you a budget microscope as well as a quality magnifying glass]. They also offer free demonstrations or models on approval for assessment at school. So search the US sites for similar school/home suppliers and they should provide excellent support for the 'best' microscope for your children's age and price range. eBay is a very cheap source of decent magnifying glasses - although they might not be finished as well as more expensive versions.
Our Royal Microscopy Society has microscope loan kits available for Primary schools to borrow and they subsidise the purchase of microscopes for Primary schools - they recommend the cheap and basic DM1 x20 monocular microscope for looking at whole objects [although the similar DM5 has the LED light source and integral batteries that you require, see: http://www.educationalmicroscopes.co.uk/dm5.html - although it's incident (above) not transmission]. Perhaps there's similar discount/loan schemes in the US. The DM1 can also be fitted with extras like an attachment for a digital camera. The DM1 is similar to a lab low power stereo [3D] 'dissecting' microscope, but without the 3D effect to make it easier (and cheaper) for youngsters to use. Compound [looking at glass slides] microscopes like the Brunel DM1 aren't so good for Elementary school kids, although perhaps one with a few interesting flat samples might be useful in the corner for structured demonstrations to small groups from Year 6 [10-11 year olds].
The DM1 is a UK Brunel branded microscope, although in the US the Omano M185 monocular microscope at www.microscope.com looks uncannily similar and clearly is the same microscope http://www.microscope.com/omano-om185-monocular-dissecting-microscope-p-414. html However I can't find a battery/Led light Brunel DM5 version in the US [Brunel or microscope.com can probably advise - or you could use a cheap small bendy stalk mains/12v halogen desk light, dropping the bulb power from 20w to 10w]. There's also http://schoolmicroscope.com, and http://www.dissectingmicroscope.com/important-features-to-look-for-in-a-qual ity-dissecting-microscope.html but I cant find the DM5 equivalent there either.
Youngsters [under 10s] often have real problems using optical technology designed for adults, and it's not just binocular eyepieces - give then a digital SLR or compact camera and invariably the photos will be blurred and useless, even with 1 to 1 adult supervision. Looking down a microscope they can have trouble finding the focus, even with a monocular microscope, and will often tell you they see something when they can't [probably in an attempt to get rid of you] - although when their face lights up you know they have finally found it.
Personally for home use I prefer video PC based microscopes that can be more fun for younger kids and these can also give them a thing they love - the photo keepsake to paste into their notebooks. Disadvantages: adults frown on the often very poor image quality, they will need full adult supervision or a demonstration unless ruggedised, and the CMOS chips/software rapidly dates [no problem for home use as your kids get bored with them very quickly once they have tried everything/done everything, but at school you want them to last for the next few batches of kids coming along]. The most successful video microscope systems for kids under 10 I have seen in UK museum and national science centres are based around expensive ruggedised [Perspex shielded] CCTV video camera microscopes with simple focussing. There are PC USB2 or CCTV based cameras you can add to microscopes, although they require a PC/TV and may be only suitable for demos by adults. Plus there's the £300 DinoLite + Stand (never tried the range or the far cheaper, and no doubt far nastier, £50 generic versions - but these are based on cheap low-res PC Skype WebCam type camera chips and also may be far too delicate and fiddly for preteens). There's also the £280 Motic DS300 or DS-2 PC based USB microscopes, all of which will probably be outside your budget (although one for a small group in rotation will be more affordable). However the image quality of the Motic DS300 or DS-2 is little better than the obsolete £80 640x480 pixel Digital Blue QX-5 USB2 microscope (although an updated Digital Blue QX-7 with 1280x1024 pixels and glass lenses was slated for production in early 2012 and hopefully will arrive soon).
However video microscopes are a bit of a distraction, so for your Elementary school classes the Brunel DM1/DM5 (Omano M185) type of microscope is probably the best option with youngsters, for price, robustness and letting the kids get involved hands on. Try one with your class on approval if you can.
Hope this helps and good luck with the science lab.
Regards
Keith
PS. I have written a simple pdf on buying a cheap microscope for home use that I regularly update [based on similar discussions many years ago on the microscopy.com listserver]: http://www.well.ox.ac.uk/_asset/file/buying-a-cheap-microscope-for-home.pdf and it has links to other sites specifically discussing microscopes for kids [for home use] - I will expand it soon to give ideas on unusual samples, etc.. [more for older kids though].
It's on our "Optical Microscope Enthusiast Sites" section of http://www.well.ox.ac.uk/external-website-links that has further links to a few microscopy educational sites
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu] Sent: 13 December 2011 17:11 To: kjmorris-at-well.ox.ac.uk
This should fit in with the current thread about microscopes for children. Please note that it is for a specifc recommendation of brand for an elementary school.
**************************************************************************** *********** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************** ************ } Date: Tue, 13 Dec 2011 08:58:19 -0800 } Reply-To: Jennifer Buchanan {jen.buchanan-at-dmr.ms.gov} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Tuesday, December 13, } 2011 at 08:58:18 AM. } } realname - Jennifer Buchanan } Email - jen.buchanan-at-dmr.ms.gov } ORGANIZATION - Grand Bay National Estuarine Research Reserve } EDUCATION - K-8 Grade Grammar School } QUESTION - I just read on this site that a monocular scope is better } for younger children. I have a small grant to buy some microscopes } for a local elementary school that is setting up a science lab. I } would like a recommendation for a monocular dissecting scope with } LED and rechargeable batteries. Any suggestions? } --
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Tue Dec 13 11:06:25 2011 2, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDH6PrN002582 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 11:06:25 -0600 2, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 2, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pBDH6OuJ015699 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 12:06:24 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 13 Dec 2, 24 -- 2011 12:06:24 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240816cb0d35f83b75-at-[141.209.160.249]} 2, 24 -- Date: Tue, 13 Dec 2011 12:06:22 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: Monocular dissecting 'scope for elementary school 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 02G8F6oCO - b98066141cc2 - 20111213 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 24 -- From kjmorris-at-well.ox.ac.uk Thu Dec 15 04:47:04 2011 26, 24 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 26, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBFAl3k2019580 26, 24 -- for {microscopy-at-microscopy.com} ; Thu, 15 Dec 2011 04:47:03 -0600 26, 24 -- Received: from dhcp432.well.ox.ac.uk ([129.67.46.19] helo=CytoWhizz) 26, 24 -- by morse.well.ox.ac.uk with esmtp (Exim 4.72) 26, 24 -- (envelope-from {kjmorris-at-well.ox.ac.uk} ) 26, 24 -- id 1Rb8q5-0001Hx-2F; Thu, 15 Dec 2011 10:47:01 +0000 26, 24 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 26, 24 -- To: {microscopy-at-microscopy.com} 26, 24 -- Cc: {jen.buchanan-at-dmr.ms.gov} 26, 24 -- References: {201112131711.pBDHBGfX011317-at-ns.microscopy.com} 26, 24 -- In-Reply-To: {201112131711.pBDHBGfX011317-at-ns.microscopy.com} 26, 24 -- Subject: RE: [Microscopy] Monocular dissecting 'scope for elementary school 26, 24 -- Date: Thu, 15 Dec 2011 10:47:01 -0000 26, 24 -- Message-ID: {000301ccbb16$e0897080$a19c5180$-at-ox.ac.uk} 26, 24 -- MIME-Version: 1.0 26, 24 -- Content-Type: text/plain; 26, 24 -- charset="iso-8859-1" 26, 24 -- X-Mailer: Microsoft Office Outlook 12.0 26, 24 -- Thread-Index: Acy5ujmzFl25f5gqSniki0mXtYdhCQAlOPzQ 26, 24 -- Content-Language: en-gb 26, 24 -- Content-Transfer-Encoding: 8bit 26, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBFAl3k2019580 ==============================End of - Headers==============================
Sorry I noted an error in the post after sending: The monocular compound microscope I briefly mentioned is the Brunel RM1 [not DM1] - I should have said: "The Compound [looking at glass slides] microscopes like the Brunel RM1 aren't so good for Elementary school kids, although perhaps one with a few interesting flat samples might be useful in the corner for structured demonstrations to small groups from Year 6 [10-11 year olds]."
--------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom.
-----Original Message----- X-from: Keith Morris [mailto:kjmorris-at-well.ox.ac.uk] Sent: 15 December 2011 10:47 To: 'microscopy-at-microscopy.com' Cc: 'jen.buchanan-at-dmr.ms.gov'
This should fit in with the current thread about microscopes for children. Please note that it is for a specifc recommendation of brand for an elementary school.
**************************************************************************** *********** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************** ************ } Date: Tue, 13 Dec 2011 08:58:19 -0800 } Reply-To: Jennifer Buchanan {jen.buchanan-at-dmr.ms.gov} } To: {oshel1pe-at-cmich.edu} } Subject: Ask-A-Microscopist } } Below is the result of your form, submitted on Tuesday, December 13, } 2011 at 08:58:18 AM. } } realname - Jennifer Buchanan } Email - jen.buchanan-at-dmr.ms.gov } ORGANIZATION - Grand Bay National Estuarine Research Reserve } EDUCATION - K-8 Grade Grammar School } QUESTION - I just read on this site that a monocular scope is better } for younger children. I have a small grant to buy some microscopes } for a local elementary school that is setting up a science lab. I } would like a recommendation for a monocular dissecting scope with } LED and rechargeable batteries. Any suggestions? } --
==============================Original Headers============================== 2, 24 -- From oshel1pe-at-cmich.edu Tue Dec 13 11:06:25 2011 2, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 2, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDH6PrN002582 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 11:06:25 -0600 2, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 2, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pBDH6OuJ015699 2, 24 -- for {Microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 12:06:24 -0500 2, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 2, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Tue, 13 Dec 2, 24 -- 2011 12:06:24 -0500 2, 24 -- MIME-Version: 1.0 2, 24 -- Message-ID: {a06240816cb0d35f83b75-at-[141.209.160.249]} 2, 24 -- Date: Tue, 13 Dec 2011 12:06:22 -0500 2, 24 -- To: {Microscopy-at-microscopy.com} 2, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 2, 24 -- Subject: Monocular dissecting 'scope for elementary school 2, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 2, 24 -- X-Originating-IP: [141.209.160.249] 2, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 2, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 2, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 2, 24 -- X-CanItPRO-Stream: default 2, 24 -- X-Canit-Stats-ID: 02G8F6oCO - b98066141cc2 - 20111213 2, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
==============================Original Headers============================== 34, 22 -- From kjmorris-at-well.ox.ac.uk Thu Dec 15 04:56:15 2011 34, 22 -- Received: from morse.well.ox.ac.uk (morse.well.ox.ac.uk [129.67.44.2]) 34, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBFAuD89026303 34, 22 -- for {microscopy-at-microscopy.com} ; Thu, 15 Dec 2011 04:56:14 -0600 34, 22 -- Received: from dhcp432.well.ox.ac.uk ([129.67.46.19] helo=CytoWhizz) 34, 22 -- by morse.well.ox.ac.uk with esmtp (Exim 4.72) 34, 22 -- (envelope-from {kjmorris-at-well.ox.ac.uk} ) 34, 22 -- id 1Rb8yz-0001Vk-FX; Thu, 15 Dec 2011 10:56:13 +0000 34, 22 -- From: "Keith Morris" {kjmorris-at-well.ox.ac.uk} 34, 22 -- To: {jen.buchanan-at-dmr.ms.gov} 34, 22 -- Cc: {microscopy-at-microscopy.com} 34, 22 -- Subject: FW: [Microscopy] Monocular dissecting 'scope for elementary school 34, 22 -- Date: Thu, 15 Dec 2011 10:56:14 -0000 34, 22 -- Message-ID: {000401ccbb18$29c53120$7d4f9360$-at-ox.ac.uk} 34, 22 -- MIME-Version: 1.0 34, 22 -- Content-Type: text/plain; 34, 22 -- charset="iso-8859-1" 34, 22 -- X-Mailer: Microsoft Office Outlook 12.0 34, 22 -- Thread-Index: Acy5ujmzFl25f5gqSniki0mXtYdhCQAlOPzQADIO4pA= 34, 22 -- Content-Language: en-gb 34, 22 -- Content-Transfer-Encoding: 8bit 34, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBFAuD89026303 ==============================End of - Headers==============================
Keith Morris's comments are excellent. The Microscopy Society of America has a similar outreach program, Project MICRO, and its web page http://www.microscopy.org/education/projectMICRO includes very similar advice, a reviewed booklist, and other resources.. One of MSA's regional societies (New England) has a few kits available for loan to teachers within their area.
The 20x monocular dissecting scope that Keith (and MICRO) recommends is indeed the same scope as the UK model; the key to look for is "model 185" which seems to remain constant even when the importer's brand name changes. The price is $75-85. A nice LED "booklight" is $8.50 on Amazon. There will be a hands-on workshop for teachers and meeting attendees at M&M 2012 in Phoenix next August; it will use this equipment.
As Keith says, understanding the concept of "focus" isn't intuitive for youngsters. You'll find good advice on how to teach it in the booklet "Introducing Pre-Kindergartners to 'The Private Eye'", published by Private Eye, www.theprivateeye.com.
Caroline
} ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
-- Caroline Schooley Project MICRO Coordinator Microscopy Society of America 45301 Caspar Point Road, Box 117 Caspar, CA 95420 Phone/FAX (707)964-9460 Project MICRO: http://www.microscopy.org/education/projectMICRO
==============================Original Headers============================== 7, 19 -- From schooley-at-mcn.org Thu Dec 15 12:24:58 2011 7, 19 -- Received: from smtp2.mcn.org (smtp2.mcn.org [216.150.240.86]) 7, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBFIOtwn030638 7, 19 -- for {microscopy-at-microscopy.com} ; Thu, 15 Dec 2011 12:24:57 -0600 7, 19 -- Received: from [66.81.42.5] 7, 19 -- by smtp2.mcn.org with esmtpa (Exim 4.76) 7, 19 -- (envelope-from {schooley-at-mcn.org} ) 7, 19 -- id 1RbFz1-0007JJ-5N; Thu, 15 Dec 2011 10:24:54 -0800 7, 19 -- Mime-Version: 1.0 7, 19 -- Message-Id: {a06240803cb0fe59a8ce1-at-[66.81.41.134]} 7, 19 -- In-Reply-To: {201112151101.pBFB1Bkg005666-at-ns.microscopy.com} 7, 19 -- References: {201112151101.pBFB1Bkg005666-at-ns.microscopy.com} 7, 19 -- Date: Thu, 15 Dec 2011 10:43:30 -0800 7, 19 -- To: kjmorris-at-well.ox.ac.uk, microscopy-at-microscopy.com 7, 19 -- From: Caroline Schooley {schooley-at-mcn.org} 7, 19 -- Subject: [Microscopy] RE: Monocular dissecting 'scope for elementary school 7, 19 -- Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" 7, 19 -- Content-Transfer-Encoding: 8bit 7, 19 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBFIOtwn030638 ==============================End of - Headers==============================
Forwarded by request. } Sender: Leona Cohen-Gould {lcgould-at-med.cornell.edu} } From: Leona Cohen-Gould {lcgould-at-med.cornell.edu} } Subject: CAP certification question } Date: Thu, 15 Dec 2011 13:22:07 -0500 } } I found out more, useful information about the CAP certification } requirements. This info was provided by a past-president of the } Renal Pathologists' group. } Two years ago, in response to concerns voiced by nephrologists over } who was reading/interpreting the EM and other test results for their } patients, CAP began to develop guidelines for the certification of } RENAL PATHOLOGISTS (MDs). The certification process has not yet } been finalized, but it is known that they do not include the } technologists preparing the samples. } } Thus, CEMT from MSA is good, but not necessarily required by CAP. } } } Lee Cohen-Gould, M.S., C.E.M.T. } Chair, MSA Certification Board } } Sr. Staff Associate in Biochemistry and } Cell & Developmental Biology } Director, Electron Microscopy & Histology } and Optical Microscopy Core Facilities } Weill Cornell Medical College } } lcgould-at-med.cornell.edu } voice (212)746-6146 } fax (212)746-8175 } http://www.med.cornell.edu/research/rea_sup/ } http://www.cornellbiochem.org } http://www.cornellcelldevbiology.org }
==============================Original Headers============================== 1, 24 -- From oshel1pe-at-cmich.edu Thu Dec 15 12:59:51 2011 1, 24 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25]) 1, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBFIxojX016253 1, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Dec 2011 12:59:51 -0600 1, 24 -- Received: from cas1.central.cmich.local (mail.cmich.edu [141.209.15.40]) 1, 24 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-5+lenny1) with ESMTP id pBFIxkFp002988 1, 24 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Dec 2011 13:59:50 -0500 1, 24 -- Received: from [141.209.160.249] (141.209.160.249) by cas1.central.cmich.local 1, 24 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.1.323.3; Thu, 15 Dec 1, 24 -- 2011 13:59:47 -0500 1, 24 -- MIME-Version: 1.0 1, 24 -- Message-ID: {a0624080fcb0ff3f0de1e-at-[141.209.160.249]} 1, 24 -- Date: Thu, 15 Dec 2011 13:59:43 -0500 1, 24 -- To: {Microscopy-at-microscopy.com} 1, 24 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 1, 24 -- Subject: Fwd: CAP certification question 1, 24 -- Content-Type: text/plain; charset="us-ascii"; format=flowed 1, 24 -- X-Originating-IP: [141.209.160.249] 1, 24 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default) 1, 24 -- X-Spam-Score: -0.50 () [Hold at 6.00] _L_LLEXCH,Bayes(0.0001:-0.5) 1, 24 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=MI; city=Mount Pleasant; postalcode=48859; latitude=43.5647; longitude=-84.8473; metrocode=513; areacode=989; http://maps.google.com/maps?q=43.5647,-84.8473&z=6 1, 24 -- X-CanItPRO-Stream: default 1, 24 -- X-Canit-Stats-ID: 02G9uXO75 - 1a5826d69f5c - 20111215 1, 24 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25 ==============================End of - Headers==============================
I missed the temperature for LR White that was suggested. In that I am doing my first exp. With it in 20 years I am wondering just what temp. is considered good now.
Pat Connelly
=====
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
==============================Original Headers============================== 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 15:18:50 2011 10, 25 -- Received: from znl.com ([206.69.208.20]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBGLIowG000728 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 10, 25 -- Received: from localhost (localhost [127.0.0.1]) 10, 25 -- by znl.com (Postfix) with ESMTP id B303545909D 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 25 -- Received: from znl.com ([127.0.0.1]) 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id rVddmf74j6Lh for {microscopy-at-microscopy.com} ; 10, 25 -- Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 25 -- by znl.com (Postfix) with ESMTPA id 6F920459091 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- Message-ID: {4EEBB5BA.2060409-at-microscopy.com} 10, 25 -- Date: Fri, 16 Dec 2011 15:18:50 -0600 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 25 -- MIME-Version: 1.0 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 25 -- Subject: [Filtered] Re: [Microscopy] viaWWW:PTFE Flat Embedding Mold 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
there are other negative stains. or use low dose (increase contrast in the tem) and high tilts. more ways to skin the cat.
On Tue, Dec 13, 2011 at 9:28 AM, {microscopylistserver-noreply-at-microscopy.com} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both tchou-at-stevens.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: tchou-at-stevens.edu Name: Alex Chou } } Organization: Stevens Tech } } Title-Subject: [Filtered] UA stain on DNA nanoparticles for TEM imaging } } Message: We are trying to image DNA nanoparticles in TEM via UA } staining. It turns out the DNA particles aggregated heavily via the UA } solution I made out of the regular DI water. According to my } collaborator, the UA solution I made has PH ~ 3 which causes the } nano-particles stick together. We tried to adjust the PH, but UA } solution starts to show sign of precipitation sooner it reaches to PH } ~6. But, we really need something that has PH around 7 to 8! So, the } questions are: } } 1. is there a way to adjust UA solution to PH around 7 to 8? } } 2. or, is there another stain solution that has PH ~ 7 to 8 we can used } for imaging DNA structures? } } Thanks, } } Alex } } Login Host: 155.246.236.151 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Dec 13 10:18:15 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBDGIFcX015854 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:18:15 -0600 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 43D2A456906 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:18:15 -0600 (CST) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 12, 25 -- with ESMTP id TO4xlNfZtUSc for {microscopy-at-microscopy.com} ; } 12, 25 -- Tue, 13 Dec 2011 10:18:13 -0600 (CST) } 12, 25 -- Received: from NestorPwrBk.local (unknown [193.173.39.42]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 4921E4568FB } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:18:12 -0600 (CST) } 12, 25 -- Message-ID: {4EE77AC2.5040106-at-microscopy.com} } 12, 25 -- Date: Tue, 13 Dec 2011 17:18:10 +0100 } 12, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: tchou-at-stevens.edu } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: [Filtered] viaWWW:UA stain on DNA nanoparticles for TEM imaging } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 5, 28 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 15:19:11 2011 5, 28 -- Received: from znl.com ([206.69.208.20]) 5, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBGLJB4F000987 5, 28 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:19:11 -0600 5, 28 -- Received: from localhost (localhost [127.0.0.1]) 5, 28 -- by znl.com (Postfix) with ESMTP id C508B4590A9 5, 28 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:19:11 -0600 (CST) 5, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 5, 28 -- Received: from znl.com ([127.0.0.1]) 5, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 5, 28 -- with ESMTP id lSWLqoXpH0PM for {microscopy-at-microscopy.com} ; 5, 28 -- Fri, 16 Dec 2011 15:19:10 -0600 (CST) 5, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 5, 28 -- by znl.com (Postfix) with ESMTPA id A19CA45909E 5, 28 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:19:10 -0600 (CST) 5, 28 -- Message-ID: {4EEBB5CE.7090703-at-microscopy.com} 5, 28 -- Date: Fri, 16 Dec 2011 15:19:10 -0600 5, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 5, 28 -- Reply-To: Robert Derby {derbyrobert-at-gmail.com} 5, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 5, 28 -- MIME-Version: 1.0 5, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 5, 28 -- Subject: [Filtered] Re: [Microscopy] viaWWW:UA stain on DNA nanoparticles 5, 28 -- for TEM imaging 5, 28 -- References: {201112131628.pBDGSsEj000404-at-ns.microscopy.com} 5, 28 -- In-Reply-To: {201112131628.pBDGSsEj000404-at-ns.microscopy.com} 5, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 5, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Try using 2.0% phosphotungstic acid in water, adjust pH to 6 or 7. Karen Bentley University of Rochester Medical Center Rochester, NY
-----Original Message----- } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Tuesday, December 13, 2011 11:26 AM To: Bentley, Karen
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both tchou-at-stevens.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: tchou-at-stevens.edu Name: Alex Chou
Organization: Stevens Tech
Title-Subject: [Filtered] UA stain on DNA nanoparticles for TEM imaging
Message: We are trying to image DNA nanoparticles in TEM via UA staining. It turns out the DNA particles aggregated heavily via the UA solution I made out of the regular DI water. According to my collaborator, the UA solution I made has PH ~ 3 which causes the nano-particles stick together. We tried to adjust the PH, but UA solution starts to show sign of precipitation sooner it reaches to PH ~6. But, we really need something that has PH around 7 to 8! So, the questions are:
1. is there a way to adjust UA solution to PH around 7 to 8?
2. or, is there another stain solution that has PH ~ 7 to 8 we can used for imaging DNA structures?
If you put DNA nanoparticles on the glow discharged carbon membrane first, then add UA as routine method to do negative staining, I don't think the nanoparticles would aggregate.
Alice
} From: "microscopylistserver-noreply-at-microscopy.com" } {microscopylistserver-noreply-at-microscopy.com} } Reply-To: "microscopylistserver-noreply-at-microscopy.com" } {microscopylistserver-noreply-at-microscopy.com} } Date: Tue, 13 Dec 2011 11:32:26 -0500 } To: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} } Subject: [Microscopy] viaWWW:UA stain on DNA nanoparticles for TEM imaging } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both tchou-at-stevens.edu as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: tchou-at-stevens.edu Name: Alex Chou } } Organization: Stevens Tech } } Title-Subject: [Filtered] UA stain on DNA nanoparticles for TEM imaging } } Message: We are trying to image DNA nanoparticles in TEM via UA } staining. It turns out the DNA particles aggregated heavily via the UA } solution I made out of the regular DI water. According to my } collaborator, the UA solution I made has PH ~ 3 which causes the } nano-particles stick together. We tried to adjust the PH, but UA } solution starts to show sign of precipitation sooner it reaches to PH } ~6. But, we really need something that has PH around 7 to 8! So, the } questions are: } } 1. is there a way to adjust UA solution to PH around 7 to 8? } } 2. or, is there another stain solution that has PH ~ 7 to 8 we can used } for imaging DNA structures? } } Thanks, } } Alex } } Login Host: 155.246.236.151 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Dec 13 10:18:15 } 2011 } 12, 25 -- Received: from znl.com ([206.69.208.20]) } 12, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } pBDGIFcX015854 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:18:15 -0600 } 12, 25 -- Received: from localhost (localhost [127.0.0.1]) } 12, 25 -- by znl.com (Postfix) with ESMTP id 43D2A456906 } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:18:15 -0600 } (CST) } 12, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 12, 25 -- Received: from znl.com ([127.0.0.1]) } 12, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } 10024) } 12, 25 -- with ESMTP id TO4xlNfZtUSc for {microscopy-at-microscopy.com} ; } 12, 25 -- Tue, 13 Dec 2011 10:18:13 -0600 (CST) } 12, 25 -- Received: from NestorPwrBk.local (unknown [193.173.39.42]) } 12, 25 -- by znl.com (Postfix) with ESMTPA id 4921E4568FB } 12, 25 -- for {microscopy-at-microscopy.com} ; Tue, 13 Dec 2011 10:18:12 -0600 } (CST) } 12, 25 -- Message-ID: {4EE77AC2.5040106-at-microscopy.com} } 12, 25 -- Date: Tue, 13 Dec 2011 17:18:10 +0100 } 12, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 12, 25 -- Reply-To: tchou-at-stevens.edu } 12, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 12, 25 -- MIME-Version: 1.0 } 12, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 25 -- Subject: [Filtered] viaWWW:UA stain on DNA nanoparticles for TEM imaging } 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 12, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. =================================
==============================Original Headers============================== 10, 26 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 15:19:42 2011 10, 26 -- Received: from znl.com ([206.69.208.20]) 10, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBGLJg5P002041 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:19:42 -0600 10, 26 -- Received: from localhost (localhost [127.0.0.1]) 10, 26 -- by znl.com (Postfix) with ESMTP id 42E614590C1 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:19:42 -0600 (CST) 10, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 26 -- Received: from znl.com ([127.0.0.1]) 10, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 26 -- with ESMTP id 5118u+BJFKc6 for {microscopy-at-microscopy.com} ; 10, 26 -- Fri, 16 Dec 2011 15:19:41 -0600 (CST) 10, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 26 -- by znl.com (Postfix) with ESMTPA id D856C4590B6 10, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:19:41 -0600 (CST) 10, 26 -- Message-ID: {4EEBB5ED.4040102-at-microscopy.com} 10, 26 -- Date: Fri, 16 Dec 2011 15:19:41 -0600 10, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 26 -- Reply-To: "Liang, Fengxia" {Fengxia.Liang-at-med.nyu.edu} 10, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 26 -- MIME-Version: 1.0 10, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 26 -- Subject: [Filtered] Re: [Microscopy] viaWWW:UA stain on DNA nanoparticles 10, 26 -- for TEM imaging 10, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
There is still one company I know that supports the AMRAY line of SEM's, SEMTechSolutions http://www.semtechsolutions.com/. This company was founded by ex-AMRAY employees and still offers sales and support for AMRAY's. I had two AMRAYS before I retired and after AMRAY left the analytical SEM market I used them frequently when I needed service or parts.
Contact Jim Peterson at jimp-at-semtechsolutions.com for more information.
I have no vested interest in in SEMTechSolutions.
Good Luck, Lou
On 12/9/11 3:04 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both stevebuckingham-at-comcast.net as well as the } MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: stevebuckingham-at-comcast.net Name: Steve Buckingham } } Organization: Satki3 } } Title-Subject: [Filtered] Amray 1860 FE Scanning Electron Microscope support } } Message: I'm thinking of purchasing a used Amray 1860 FE Scanning } Electron Microscope but I am concerned that there may not be support or } parts still available for these systems. The system is in full working } condition right now and can be demonstrated for performance. } However, I would be interested to know what kind of support still exists } for repair of these systems along with information on the availability } of parts. } Many Thanks, } Steve } } Login Host: 68.61.156.255 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 9 03:01:32 } 2011 } 8, 25 -- Received: from znl.com ([206.69.208.20]) } 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } pB991WD8025987 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:32 -0600 } 8, 25 -- Received: from localhost (localhost [127.0.0.1]) } 8, 25 -- by znl.com (Postfix) with ESMTP id 88550453501 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:32 -0600 } (CST) } 8, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 8, 25 -- Received: from znl.com ([127.0.0.1]) } 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port } 10024) } 8, 25 -- with ESMTP id 27dDULW1os2z for {microscopy-at-microscopy.com} ; } 8, 25 -- Fri, 9 Dec 2011 03:01:31 -0600 (CST) } 8, 25 -- Received: from NestorPwrBk.local (83.138.221.115.dyn.user.ono.com } [83.138.221.115]) } 8, 25 -- by znl.com (Postfix) with ESMTPA id C1D1C4534F6 } 8, 25 -- for {microscopy-at-microscopy.com} ; Fri, 9 Dec 2011 03:01:30 -0600 } (CST) } 8, 25 -- Message-ID: {4EE1CE69.9000702-at-microscopy.com} } 8, 25 -- Date: Fri, 09 Dec 2011 10:01:29 +0100 } 8, 25 -- From: MicroscopyListserver-NoReply } {microscopylistserver-noreply-at-microscopy.com} } 8, 25 -- Reply-To: stevebuckingham-at-comcast.net } 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; } rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 8, 25 -- MIME-Version: 1.0 } 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 25 -- Subject: [Filtered] viaWWW:Amray 1860 FE Scanning Electron Microscope support } 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 8, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 12, 26 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 15:20:13 2011 12, 26 -- Received: from znl.com ([206.69.208.20]) 12, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBGLKDKh003283 12, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:20:13 -0600 12, 26 -- Received: from localhost (localhost [127.0.0.1]) 12, 26 -- by znl.com (Postfix) with ESMTP id 32B174590CE 12, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:20:13 -0600 (CST) 12, 26 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 26 -- Received: from znl.com ([127.0.0.1]) 12, 26 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 26 -- with ESMTP id swd3hWNmTVsD for {microscopy-at-microscopy.com} ; 12, 26 -- Fri, 16 Dec 2011 15:20:11 -0600 (CST) 12, 26 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 26 -- by znl.com (Postfix) with ESMTPA id B0AA84590C3 12, 26 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:20:11 -0600 (CST) 12, 26 -- Message-ID: {4EEBB60B.2000102-at-microscopy.com} 12, 26 -- Date: Fri, 16 Dec 2011 15:20:11 -0600 12, 26 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 26 -- Reply-To: "Ross Jr, Louis M." {RossLM-at-missouri.edu} 12, 26 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 26 -- MIME-Version: 1.0 12, 26 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 26 -- Subject: [Filtered] Re: [Microscopy] viaWWW:Amray 1860 FE Scanning Electron 12, 26 -- Microscope support 12, 26 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 26 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both mlibbee-at-gmail.com as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
I can't figure out why I am not able to image in darkfield mode with our BX60 Olympus microscope. I've followed the steps outlined in the manual a few times and think that the 'cube selector' or Brightfield/Darkfield Vertical Illuminator Attachment is out of alignment. During the final step, I can briefly see the image of the DF insert on the sample: central light is blocked and the oblique angles of light pass quickly over the surface of the sample. If anyone has attempted to correct/align the mirror cube location for proper light path selection, will you please contact me about your recommendations and experience?
LR white has to be polymerized in beem capsules or gelatin capsules (air tight) and at 45degrees if using for IMMUNO EM. It would polymerize even at higher otherwise.
Shashi Singh Scientist Centre for Cellular and Molecular Biology Hyderabad-500 007 INDIA PH-91-40-27192575,27192764 FAX-91-40-27160591, 27160311 Mobile- 91-98660-98626
X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: Shashis_99-at-yahoo.com Sent: Saturday, December 17, 2011 2:52 AM
Tami,
I missed the temperature for LR White that was suggested. In that I am doing my first exp. With it in 20 years I am wondering just what temp. is considered good now.
Pat Connelly
=====
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
==============================Original Headers============================== 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 15:18:50 2011 10, 25 -- Received: from znl.com ([206.69.208.20]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBGLIowG000728 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 10, 25 -- Received: from localhost (localhost [127.0.0.1]) 10, 25 -- by znl.com (Postfix) with ESMTP id B303545909D 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 25 -- Received: from znl.com ([127.0.0.1]) 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id rVddmf74j6Lh for {microscopy-at-microscopy.com} ; 10, 25 -- Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 25 -- by znl.com (Postfix) with ESMTPA id 6F920459091 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- Message-ID: {4EEBB5BA.2060409-at-microscopy.com} 10, 25 -- Date: Fri, 16 Dec 2011 15:18:50 -0600 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 25 -- MIME-Version: 1.0 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 25 -- Subject: [Filtered] Re: [Microscopy] viaWWW:PTFE Flat Embedding Mold 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Dec 17 11:00:12 2011 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBHH0Cfu020788 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 11:00:12 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 6949F459ADF 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 11:00:12 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id RPf+D+Bmn8dl for {microscopy-at-microscopy.com} ; 19, 28 -- Sat, 17 Dec 2011 11:00:09 -0600 (CST) 19, 28 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 64979459AD4 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 11:00:09 -0600 (CST) 19, 28 -- Message-ID: {4EECCA99.8080009-at-microscopy.com} 19, 28 -- Date: Sat, 17 Dec 2011 11:00:09 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: shashi singh {shashis_99-at-yahoo.com} 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: [Filtered] Re: [Microscopy] [Filtered] Re: viaWWW:PTFE Flat Embedding 19, 28 -- Mold 19, 28 -- References: {201112162122.pBGLMXZE010267-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201112162122.pBGLMXZE010267-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Just came across an article on Popular Science, describing the plenoptics camera, which has an array of micro-lenses in front of the image sensor for 3D imaging as well "perfect focusing".
We routinely polymerise LRWhite at about 60 C under nitrogen gas so no need to seal the containers. A 2-3 mm deep mould of medium grade resin takes about 90 min to polymerise this way when it's an aluminium mould. When we use any of the plastic moulds it takes about double this time to polymerise, I don't really understand why though could speculate... Resin polymerised at this temperature is fine for immuno work, at least in plant material. You can also polymerise it under UV either sealed or under nitrogen, but our OHS people prefer us not to use UV these days. cheers,
Rosemary White CSIRO Plant Industry GPO Box 1600 Canberra, ACT 2601 Australia
T 61 2 6246 5475 F 61 2 6246 5334 E rosemary.white-at-csiro.au
On 18/12/11 4:07 AM, "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} wrote:
} } } } -------------------------------------------------------------------------- } -- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
==============================Original Headers============================== 10, 31 -- From prvs=326d4c0a4=Rosemary.White-at-csiro.au Sat Dec 17 20:28:37 2011 10, 31 -- Received: from act-MTAout6.csiro.au (act-MTAout6.csiro.au [150.229.7.43]) 10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBI2SZlK030746 10, 31 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 20:28:36 -0600 10, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple; 10, 31 -- d=csiro.au; i=rosemary.white-at-csiro.au; q=dns/txt; 10, 31 -- s=email; t=1324175317; x=1355711317; 10, 31 -- h=date:subject:from:to:message-id:in-reply-to:mime-version: 10, 31 -- content-transfer-encoding; 10, 31 -- bh=+lwerCnDLr9Xqao7hjOnZAtY5UlzzCsBwHK1zuWnUYs=; 10, 31 -- b=nSITzUzzIn8YydKiBpQgChEhmZQ0o5bNRkIHt/yHEelgQfPeuunCC3A4 10, 31 -- oa+ZIWkaN/Fvh8wp8zk87o9wF8h28at2dRLKZ+HQT0vxvQLw3ujTwId7/ 10, 31 -- PFH+VyddSnwuDm/; 10, 31 -- X-IronPort-AV: E=Sophos;i="4.71,369,1320584400"; 10, 31 -- d="scan'208";a="36916027" 10, 31 -- Received: from exnsw-htca02.nexus.csiro.au ([130.155.117.127]) 10, 31 -- by act-ironport-int.csiro.au with ESMTP/TLS/RC4-MD5; 18 Dec 2011 13:28:31 +1100 10, 31 -- Received: from [152.83.195.117] (152.83.195.117) by 10, 31 -- EXNSW-HTCA02.nexus.csiro.au (130.155.117.127) with Microsoft SMTP Server 10, 31 -- (TLS) id 8.3.213.0; Sun, 18 Dec 2011 13:28:30 +1100 10, 31 -- User-Agent: Microsoft-MacOutlook/14.10.0.110310 10, 31 -- Date: Sun, 18 Dec 2011 13:28:27 +1100 10, 31 -- Subject: Re: viaWWW:PTFE Flat Embedding 10, 31 -- From: Rosemary White {rosemary.white-at-csiro.au} 10, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 31 -- Message-ID: {CB139965.66A48%rosemary.white-at-csiro.au} 10, 31 -- Thread-Topic: viaWWW:PTFE Flat Embedding 10, 31 -- In-Reply-To: {201112171707.pBHH7DXc028622-at-ns.microscopy.com} 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Type: text/plain; charset="US-ASCII" 10, 31 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Yes, it has. In fact, a few weeks ago, I gave a talk on the subject to the advanced microscopy class in which I'm instructional assistant. The Stanford Computer Graphics Lab, which also developed the prototype for the plenoptic Lytro consumer camera, has had a light field microscope in development for several years now:
The light field module is incorporated into the light path between the objective and the camera rather than into the camera itself. I'm not sure if the Raytrix camera has ever been used on an unmodified microscope, but considering that an optical tube presents a focal plane and multiple planes of out of focus light, I don't see why this wouldn't work. Then again, this is probably sub-optimal for light field microscopy, as the Stanford lab has gone through the trouble of putting together a full light field microscope design, even if is a fairly simple modification of an regular transmitted light/ fluorescence microscope. If you look at the other pages on the Stanford site, they note that they've added a light field illumination system to the design as well:
Light field microscopes use 3D deconvolution to get a fully focused image, which surprisingly works well with 4D light field information. In fact, because of z-resolution limitations, the ray tracing software used with light field cameras like the Lytro does not work in light field microscopy.
The Stanford light field camera and microscope design is limited in terms of spatial resolution as well, however, the light field camera project at Adobe claims to have overcome this limitation:
This is a very interesting and promising technology, and I'm anxious to see how it develops. I hope to be able to try this technology out for myself inside of a few years.
Peter G. Werner Program Assistant, Merritt College Microscopy Program
On Dec 17, 2011, at 3:59 PM, ZZhang-at-uwyo.edu wrote:
} Hi list: } } } Just came across an article on Popular Science, describing the } plenoptics camera, which has an array of micro-lenses in front of } the image sensor for 3D imaging as well "perfect focusing". } } } http://www.popsci.com/gadgets/article/2011-05/cameras-40000-lenses-help-salvage-blurry-images } } } Does anyone know if this technology have been used on any kind of } microscopes? } } } Merry Christmas! } } } Zhaojie } } } Zhaojie Zhang, Ph.D. } Director, Jenkins Microscopy Facility } University of Wyoming
==============================Original Headers============================== 16, 19 -- From germpore-at-sonic.net Sat Dec 17 23:38:58 2011 16, 19 -- Received: from a.mail.sonic.net (a.mail.sonic.net [64.142.16.245]) 16, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBI5cwKl016983 16, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 23:38:58 -0600 16, 19 -- Received: from 209-148-96-231.dial.dynamic.sonic.net (209-148-96-231.dial.dynamic.sonic.net [209.148.96.231]) 16, 19 -- (authenticated bits=0) 16, 19 -- by a.mail.sonic.net (8.13.8.Beta0-Sonic/8.13.7) with ESMTP id pBI5cj4l017471 16, 19 -- for {Microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 21:38:52 -0800 16, 19 -- Message-Id: {4FCFF6F2-0F1A-4D8A-BB8C-A083E8DAF5E8-at-sonic.net} 16, 19 -- From: Peter Werner {germpore-at-sonic.net} 16, 19 -- To: Microscopy-at-microscopy.com 16, 19 -- In-Reply-To: {201112172359.pBHNxIxM026283-at-ns.microscopy.com} 16, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 16, 19 -- Content-Transfer-Encoding: 7bit 16, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 16, 19 -- Subject: Re: [Microscopy] Plenoptics 16, 19 -- Date: Sat, 17 Dec 2011 21:38:42 -0800 16, 19 -- References: {201112172359.pBHNxIxM026283-at-ns.microscopy.com} 16, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
LR White can also be polymerized for immunoEM down to -20 C if you use the Cold Accelerator. The manufacturer recommends ~1.5 ul per ml resin, but I've found this leads to inadequate polymerization at -20 C. A more effective concentration is 5-7.5 ul/ml resin. Allow to polymerize overnight at -20 C then put on your bench top and let warm to room temp. Polymerization of LR White is always improved if the resin is degassed for ~ 10 min under at least house vacuum before use. If you are cooling the resin for low temp polymerization, also allow the resin to cool to -20 C before adding the Cold Accelerator. Richard Fetter Janelia Farm Research Campus HHMI
________________________________________ } From: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com] Sent: Saturday, December 17, 2011 12:09 PM To: Fetter, Richard
LR white has to be polymerized in beem capsules or gelatin capsules (air tight) and at 45degrees if using for IMMUNO EM. It would polymerize even at higher otherwise.
Shashi Singh Scientist Centre for Cellular and Molecular Biology Hyderabad-500 007 INDIA PH-91-40-27192575,27192764 FAX-91-40-27160591, 27160311 Mobile- 91-98660-98626
X-from: "microscopylistserver-noreply-at-microscopy.com" {microscopylistserver-noreply-at-microscopy.com} To: Shashis_99-at-yahoo.com Sent: Saturday, December 17, 2011 2:52 AM
Tami,
I missed the temperature for LR White that was suggested. In that I am doing my first exp. With it in 20 years I am wondering just what temp. is considered good now.
Pat Connelly
=====
Just a quick question: Has anyone used the PTFE flat molds sold by EMS to polymerize LRWhite resin? I would appreciate if you could share your experience on how well the resin consistently polymerizes under these conditions (ideal temperature and time of polymerization etc).
Thanks!!
Tami Bogea, PhD Research Associate University of British Columbia
==============================Original Headers============================== 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 15:18:50 2011 10, 25 -- Received: from znl.com ([206.69.208.20]) 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBGLIowG000728 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 10, 25 -- Received: from localhost (localhost [127.0.0.1]) 10, 25 -- by znl.com (Postfix) with ESMTP id B303545909D 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 10, 25 -- Received: from znl.com ([127.0.0.1]) 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 10, 25 -- with ESMTP id rVddmf74j6Lh for {microscopy-at-microscopy.com} ; 10, 25 -- Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 10, 25 -- by znl.com (Postfix) with ESMTPA id 6F920459091 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:18:50 -0600 (CST) 10, 25 -- Message-ID: {4EEBB5BA.2060409-at-microscopy.com} 10, 25 -- Date: Fri, 16 Dec 2011 15:18:50 -0600 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 10, 25 -- Reply-To: "Connelly, Patricia (NIH/NHLBI) [E]" {connellyps-at-nhlbi.nih.gov} 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 10, 25 -- MIME-Version: 1.0 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 25 -- Subject: [Filtered] Re: [Microscopy] viaWWW:PTFE Flat Embedding Mold 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 10, 25 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 19, 28 -- From microscopylistserver-noreply-at-microscopy.com Sat Dec 17 11:00:12 2011 19, 28 -- Received: from znl.com ([206.69.208.20]) 19, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBHH0Cfu020788 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 11:00:12 -0600 19, 28 -- Received: from localhost (localhost [127.0.0.1]) 19, 28 -- by znl.com (Postfix) with ESMTP id 6949F459ADF 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 11:00:12 -0600 (CST) 19, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 19, 28 -- Received: from znl.com ([127.0.0.1]) 19, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 19, 28 -- with ESMTP id RPf+D+Bmn8dl for {microscopy-at-microscopy.com} ; 19, 28 -- Sat, 17 Dec 2011 11:00:09 -0600 (CST) 19, 28 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com [206.69.208.22]) 19, 28 -- by znl.com (Postfix) with ESMTPA id 64979459AD4 19, 28 -- for {microscopy-at-microscopy.com} ; Sat, 17 Dec 2011 11:00:09 -0600 (CST) 19, 28 -- Message-ID: {4EECCA99.8080009-at-microscopy.com} 19, 28 -- Date: Sat, 17 Dec 2011 11:00:09 -0600 19, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 19, 28 -- Reply-To: shashi singh {shashis_99-at-yahoo.com} 19, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 19, 28 -- MIME-Version: 1.0 19, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 19, 28 -- Subject: [Filtered] Re: [Microscopy] [Filtered] Re: viaWWW:PTFE Flat Embedding 19, 28 -- Mold 19, 28 -- References: {201112162122.pBGLMXZE010267-at-ns.microscopy.com} 19, 28 -- In-Reply-To: {201112162122.pBGLMXZE010267-at-ns.microscopy.com} 19, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 19, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
==============================Original Headers============================== 27, 28 -- From microscopylistserver-noreply-at-microscopy.com Sun Dec 18 07:54:03 2011 27, 28 -- Received: from znl.com ([206.69.208.20]) 27, 28 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBIDs39e023993 27, 28 -- for {microscopy-at-microscopy.com} ; Sun, 18 Dec 2011 07:54:03 -0600 27, 28 -- Received: from localhost (localhost [127.0.0.1]) 27, 28 -- by znl.com (Postfix) with ESMTP id BE28745F4FD 27, 28 -- for {microscopy-at-microscopy.com} ; Sun, 18 Dec 2011 07:54:02 -0600 (CST) 27, 28 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 27, 28 -- Received: from znl.com ([127.0.0.1]) 27, 28 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 27, 28 -- with ESMTP id eGjxTX0wXbMc for {microscopy-at-microscopy.com} ; 27, 28 -- Sun, 18 Dec 2011 07:53:41 -0600 (CST) 27, 28 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 27, 28 -- by znl.com (Postfix) with ESMTPA id 457BE45F4EB 27, 28 -- for {microscopy-at-microscopy.com} ; Sun, 18 Dec 2011 07:53:41 -0600 (CST) 27, 28 -- Message-ID: {4EEDF063.6040503-at-microscopy.com} 27, 28 -- Date: Sun, 18 Dec 2011 07:53:39 -0600 27, 28 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 27, 28 -- Reply-To: "Fetter, Richard" {fetterr-at-janelia.hhmi.org} 27, 28 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 27, 28 -- MIME-Version: 1.0 27, 28 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 27, 28 -- Subject: [Filtered] RE: [Microscopy] [Filtered] Re: [Filtered] Re: viaWWW:PTFE 27, 28 -- Flat Embedding 27, 28 -- References: {201112171709.pBHH9Pp0032698-at-ns.microscopy.com} 27, 28 -- In-Reply-To: {201112171709.pBHH9Pp0032698-at-ns.microscopy.com} 27, 28 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 27, 28 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
I was kind of hoping there would be further conversation on the topic of plenoptic/light-field microscopy, since in spite of having read a bit about it from various sources, I still have many questions about it.
1) Could somebody provide me with or point me to a good intro to the concept of "light fields"? (Either web or book source is fine. There's a Wikipedia article, but I don't think it introduces the concept to beginners particularly well.) My understanding is that it is a 4- dimensional or even 5-dimensional model for light that, in addition to x, y, and z spatial information, also includes one (or sometimes two) vectors for direction of travel of a photon/light ray. But that's about the extent of what I understand it to be; I'd love to learn more about it, but will need to do so from simple geometric optics on up.
2) I've read that light-field microscopes and cameras have not only spatial resolution, like any optical instrument, but angular resolution that is inherent to the ability to detect the light field, and that there's a trade-off between the two. I'm a bit confused by this, as I've long been taught that spatial resolution is essentially a function of the angular resolution of a lens system (hence, why the same size object appears smaller at a greater distance - it intercepts a smaller angle of the eye's field of view). Any idea how angular and spatial resolution are differentiated when describing plenoptic sensing?
3) The key component in plenoptic microscopes and cameras is a microlens array. (Although there are a few alternate plenoptic camera technologies that use other interference patters to derive angular information.) Of course, many standard CCDs have microlens arrays associated with them, designed in such a way as to focus light on the sensor rather than non-sensing parts of the chip. However, the microlens array in a light-field sensor is set up in such a way as to provide angular information. How is this microlens set up different than a standard CCD microlens and how does this provide angular/4D information?
4) 4D light-field information is readily focusable using 3D deconvolution algorithms. I'm not sure why this should be and would appreciate an explanation. Also, I'm told that the reason 4D ray tracing algorithms used, for example, in the Lytro camera will not work with light-field microscopy because optical sections in the latter are too narrow to provide sufficient angular information to use such algorithms. Am I correct about this?
5) Has anybody used or heard of a Raytrix light-field camera used as a camera for an otherwise standard trinocular microscope? If so, did it work for getting light-field microscope images, or was further modification of the microscope itself needed? Was deconvolution used for focusing the image?
A lot of questions, I know, but answers to any of the above would be greatly appreciated.
Peter G. Werner Program Assistant, Merritt College Microscopy Program
==============================Original Headers============================== 10, 19 -- From germpore-at-sonic.net Mon Dec 19 00:02:07 2011 10, 19 -- Received: from a.mail.sonic.net (a.mail.sonic.net [64.142.16.245]) 10, 19 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBJ626qX018325 10, 19 -- for {Microscopy-at-microscopy.com} ; Mon, 19 Dec 2011 00:02:06 -0600 10, 19 -- Received: from 209-148-96-169.dial.dynamic.sonic.net (209-148-96-169.dial.dynamic.sonic.net [209.148.96.169]) 10, 19 -- (authenticated bits=0) 10, 19 -- by a.mail.sonic.net (8.13.8.Beta0-Sonic/8.13.7) with ESMTP id pBJ61wBV017067 10, 19 -- for {Microscopy-at-microscopy.com} ; Sun, 18 Dec 2011 22:02:03 -0800 10, 19 -- Message-Id: {CF182507-9A71-403F-8A49-7D33152789C6-at-sonic.net} 10, 19 -- From: Peter Werner {germpore-at-sonic.net} 10, 19 -- To: Microscopy-at-microscopy.com 10, 19 -- In-Reply-To: {201112180556.pBI5uRbG030774-at-ns.microscopy.com} 10, 19 -- Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes 10, 19 -- Content-Transfer-Encoding: 7bit 10, 19 -- Mime-Version: 1.0 (Apple Message framework v936) 10, 19 -- Subject: Plenoptics: Some Questions 10, 19 -- Date: Sun, 18 Dec 2011 22:01:57 -0800 10, 19 -- References: {201112180556.pBI5uRbG030774-at-ns.microscopy.com} 10, 19 -- X-Mailer: Apple Mail (2.936) ==============================End of - Headers==============================
Dear All, a very good day to all ofyou! Recently a Professor would like to use the JEOL 2100F 's electron beam ( I am in charge of this TEM) to punch a 2-5nm hole on the SiN sample or perhaps his sample grid. I would like to ask your kind advices if there is any issue in letting him to do so such as if removing that amount of SiN might contaminate the chamber/column? His sample is bought from the commercial SiN grid (http://www.2spi.com/catalog/grids/silicon-nitride.php#6)
Cheers, Yee Yan NTU, MSE FACTS
==============================Original Headers============================== 2, 37 -- From one_twinklestar-at-yahoo.com.sg Tue Dec 20 19:05:11 2011 2, 37 -- Received: from nm23-vm1.bullet.mail.sg3.yahoo.com (nm23-vm1.bullet.mail.sg3.yahoo.com [106.10.151.64]) 2, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pBL158ha024138 2, 37 -- for {microscopy-at-microscopy.com} ; Tue, 20 Dec 2011 19:05:10 -0600 2, 37 -- Received: from [106.10.166.125] by nm23.bullet.mail.sg3.yahoo.com with NNFMP; 21 Dec 2011 01:05:07 -0000 2, 37 -- Received: from [106.10.151.219] by tm14.bullet.mail.sg3.yahoo.com with NNFMP; 21 Dec 2011 01:05:07 -0000 2, 37 -- Received: from [127.0.0.1] by omp1017.mail.sg3.yahoo.com with NNFMP; 21 Dec 2011 01:05:07 -0000 2, 37 -- X-Yahoo-Newman-Property: ymail-3 2, 37 -- X-Yahoo-Newman-Id: 14172.40469.bm-at-omp1017.mail.sg3.yahoo.com 2, 37 -- Received: (qmail 87849 invoked by uid 60001); 21 Dec 2011 01:05:06 -0000 2, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1324429506; bh=0KlYR8x2XHHoahovSKTQpkSJKHO07WxmWMcPHV0OI6Q=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; b=nLhgrb4TBE/fiumKAbrg/bc1O6slK5PCqU1cCQJO2w/zwiZi/peuINmiSpUDnBYReBdOIISUfS/bgGi5AZn62O9zC4j6ozyCoxD7Lr9IZ2OgH59rmJA41X5A3FMSBfgkGSTk8Octxt3RcK34/s3OQfZ/Efc24xtPi6qqPglaoz8= 2, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 37 -- s=s1024; d=yahoo.com.sg; 2, 37 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIME-Version:Content-Type:Content-Transfer-Encoding; 2, 37 -- b=D6Cn7wz0X4MhOjM8f25utoCR6kebfh2LZgeFdPnwutRHuWZ32EOxWTZSJtzVp9CGq5VToA17n12aOfyp58yGFXwrb65YazwNJXHB5ANOtHiYRpMedRFsTRI9xq7EbwJ9Rw64CDrBCrkx9RN9rWkmByZTTSJISoN0BUBAIqwXva8=; 2, 37 -- X-YMail-OSG: Q34DfCgVM1mtYzvcPy20E5dZOI0rlFCylFAjLGdyLD6aQH. 2, 37 -- Nm7eG6AVt39BUyM9r8dBrcIWGZYg0xeeXEdlhcQgqXFGGeIxNSzMgzKCick. 2, 37 -- JKdUPwyereYFJTPe4Kzd7xkvH7yATrH7zIPQ.8pYP94WlBRRSqj.WDbtZofO 2, 37 -- veSIhPDBJzwJmSzzu0guuZIor37MWRsql33V3fGB75tM8p5PKwD2UDo.ifsw 2, 37 -- JS1ecvz_sHYMv2UhNYbusp0wwgkDwfct2iuneR3bL7GALuxVEMSxTf6j26Pk 2, 37 -- 13BLZcpYcm2SNdBknk.Dx7T8.LycfHrowz5gQCw_JZo_zvRDi7nd8pZpX7k5 2, 37 -- E0jM6_39TwgP8YfiRYmhyNaofd7lW_m9gOSfcNpRmIIJX7q_IAeVDDkqHLJx 2, 37 -- ZOYPINwPo0ZZwfdqpOyRdzPphJdkR9BcnV.mnIJOuO2OvdMHiRkEAdkkqDOO 2, 37 -- NeZBO48EaWWcu77GRI2fENy3mibA83tGEyHa5ZsFfdjug9PVR4RW5jYymn2A 2, 37 -- X 2, 37 -- Received: from [155.69.41.133] by web77402.mail.sg1.yahoo.com via HTTP; Wed, 21 Dec 2011 09:05:06 SGT 2, 37 -- X-Mailer: YahooMailWebService/0.8.115.331698 2, 37 -- Message-ID: {1324429506.87568.YahooMailNeo-at-web77402.mail.sg1.yahoo.com} 2, 37 -- Date: Wed, 21 Dec 2011 09:05:06 +0800 (SGT) 2, 37 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 2, 37 -- Reply-To: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 2, 37 -- Subject: About Punching a hole in SiN in TEM 2, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 37 -- MIME-Version: 1.0 2, 37 -- Content-Type: text/plain; charset=iso-8859-1 2, 37 -- Content-Transfer-Encoding: 8bit 2, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBL158ha024138 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both meulia.1-at-osu.edu as well as the MIcroscopy Listserver ---------------------------------------------------------------------------
Email: meulia.1-at-osu.edu Name: Tea Meulia
Organization: Ohio State University
Title-Subject: [Filtered] High Pressure Freezing Message: All,
I am applying for funding to acquire HPF instrumentation. I had the opportunity to use the Leica EM PACT2 and the BalTec HPM100 (now also Leica) instruments, and both these instruments performed great. However, price is an issue. Are there any other instruments on the market that you could recommend to me to check out? Thank you.
Tea Meulia
********************************************** Tea Meulia MCIC, Director Plant Pathology, Research Associate Professor The Ohio State University 1680 Madison Avenue Wooster Ohio 44691 Office: 330-263-3836 Lab: 330-263-3828 Fax: 330-202-3563 www.oardc.ohio-state.edu/mcic **********************************************************
Hi Tea, RMC Boeckeler is also in the market for high pressure freezing. Their site is at: http://www.rmcproducts.com/hpm-010/
Best regards, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 21.12.11 06:47, schrieb microscopylistserver-noreply-at-microscopy.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } meulia.1-at-osu.edu as well as the MIcroscopy Listserver } --------------------------------------------------------------------------- } } Email: meulia.1-at-osu.edu Name: Tea Meulia } } Organization: Ohio State University } } Title-Subject: [Filtered] High Pressure Freezing } Message: All, } } I am applying for funding to acquire HPF instrumentation. I had the opportunity to use the Leica EM } PACT2 and the BalTec HPM100 (now also Leica) instruments, and both these instruments performed } great. However, price is an issue. Are there any other instruments on the market that you could } recommend to me to check out? } Thank you. } } Tea Meulia } } ********************************************** } Tea Meulia MCIC, Director } Plant Pathology, Research Associate Professor } The Ohio State University } 1680 Madison Avenue } Wooster Ohio 44691 } Office: 330-263-3836 } Lab: 330-263-3828 } Fax: 330-202-3563 } www.oardc.ohio-state.edu/mcic } ********************************************************** } } Login Host: 164.107.87.114 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Tue Dec 20 23:41:49 2011 } 10, 25 -- Received: from znl.com ([206.69.208.20]) } 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBL5fne5015016 } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Dec 2011 23:41:49 -0600 } 10, 25 -- Received: from localhost (localhost [127.0.0.1]) } 10, 25 -- by znl.com (Postfix) with ESMTP id 79E9E461B47 } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Dec 2011 23:41:49 -0600 (CST) } 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain } 10, 25 -- Received: from znl.com ([127.0.0.1]) } 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) } 10, 25 -- with ESMTP id qi3N2ALNstwR for {microscopy-at-microscopy.com} ; } 10, 25 -- Tue, 20 Dec 2011 23:41:47 -0600 (CST) } 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) } 10, 25 -- by znl.com (Postfix) with ESMTPA id 21FB4461B3C } 10, 25 -- for {microscopy-at-microscopy.com} ; Tue, 20 Dec 2011 23:41:47 -0600 (CST) } 10, 25 -- Message-ID: {4EF1719A.8000704-at-microscopy.com} } 10, 25 -- Date: Tue, 20 Dec 2011 23:41:46 -0600 } 10, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} } 10, 25 -- Reply-To: meulia.1-at-osu.edu } 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 } 10, 25 -- MIME-Version: 1.0 } 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 25 -- Subject: viaWWW:High Pressure Freezing } 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed } 10, 25 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
==============================Original Headers============================== 6, 22 -- From stefan.diller-at-t-online.de Wed Dec 21 00:20:46 2011 6, 22 -- Received: from mailout01.t-online.de (mailout01.t-online.de [194.25.134.80]) 6, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBL6KjHn032348 6, 22 -- for {microscopy-at-microscopy.com} ; Wed, 21 Dec 2011 00:20:46 -0600 6, 22 -- Received: from fwd06.aul.t-online.de (fwd06.aul.t-online.de ) 6, 22 -- by mailout01.t-online.de with smtp 6, 22 -- id 1RdFXf-0004Of-Eu; Wed, 21 Dec 2011 07:20:43 +0100 6, 22 -- Received: from mac-pro.speedport.ip (VyQMY6ZQghiK2LSmRZUi4OWCofqCYIY6WaXwQCVL4FsWhXGxP1Bn2yO+LeHX52Ewlh-at-[87.178.233.77]) by fwd06.t-online.de 6, 22 -- with esmtp id 1RdFXZ-02N7AG0; Wed, 21 Dec 2011 07:20:37 +0100 6, 22 -- Message-ID: {4EF17AB4.9000604-at-t-online.de} 6, 22 -- Date: Wed, 21 Dec 2011 07:20:36 +0100 6, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de} 6, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:8.0) Gecko/20111105 Thunderbird/8.0 6, 22 -- MIME-Version: 1.0 6, 22 -- To: microscopy-at-microscopy.com 6, 22 -- Subject: Re: [Microscopy] viaWWW:High Pressure Freezing 6, 22 -- References: {201112210547.pBL5la94020993-at-ns.microscopy.com} 6, 22 -- In-Reply-To: {201112210547.pBL5la94020993-at-ns.microscopy.com} 6, 22 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 6, 22 -- Content-Transfer-Encoding: 7bit 6, 22 -- X-ID: VyQMY6ZQghiK2LSmRZUi4OWCofqCYIY6WaXwQCVL4FsWhXGxP1Bn2yO+LeHX52Ewlh 6, 22 -- X-TOI-MSGID: 6ae38cfb-1b14-4481-865a-fef5dd6abf4e ==============================End of - Headers==============================
} I am applying for funding to acquire HPF instrumentation. I had the } opportunity to use the Leica EM } PACT2 and the BalTec HPM100 (now also Leica) instruments, and both these } instruments performed } great. However, price is an issue. Are there any other instruments on the } market that you could } recommend to me to check out? } Thank you.
and, finally, not only the two mentioned above, and RMC, but also: Martin Wohlwend. Bifig 14. 9466 Sennwald. +41 81 759 27 65 (Tel. Geschäft). +41 81 757 19 24 (Tel. Privat). martin-wohlwend-at-bluewin.ch His machine is the compact 01, as far as I know. Probably, I am not up-to-date about the latest model that he is producing. The best is to contact him personally. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstr. 31 D-93053 Regensburg - Germany tel +49 941 943 2837, 1720 fax +49 941 943 2868 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29
next microscopy conferences: http://www.emc2012.org.uk/ EMC 2012 in Manchester, UK http://www.mc2013.de/ MC2013 in Regensburg, Germany
==============================Original Headers============================== 5, 24 -- From reinhard.rachel-at-biologie.uni-regensburg.de Wed Dec 21 02:06:47 2011 5, 24 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52]) 5, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBL86krV018084 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Dec 2011 02:06:47 -0600 5, 24 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1]) 5, 24 -- by localhost (Postfix) with SMTP id 056D6577F 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Dec 2011 09:06:43 +0100 (CET) 5, 24 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 5, 24 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id DDA43575F 5, 24 -- for {Microscopy-at-microscopy.com} ; Wed, 21 Dec 2011 09:06:42 +0100 (CET) 5, 24 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 5, 24 -- with Novell_GroupWise; Wed, 21 Dec 2011 09:06:42 +0100 5, 24 -- Message-Id: {4EF1A1A302000054000317ED-at-gwsmtp1.uni-regensburg.de} 5, 24 -- X-Mailer: Novell GroupWise Internet Agent 12.0.0 5, 24 -- Date: Wed, 21 Dec 2011 09:06:43 +0100 5, 24 -- From: "reinhard rachel" {reinhard.rachel-at-biologie.uni-regensburg.de} 5, 24 -- To: "microscopy listserver" {Microscopy-at-microscopy.com} 5, 24 -- Subject: High Pressure Freezing 5, 24 -- References: {201112210542.pBL5gBEb015330-at-ns.microscopy.com} 5, 24 -- In-Reply-To: {201112210542.pBL5gBEb015330-at-ns.microscopy.com} 5, 24 -- Mime-Version: 1.0 5, 24 -- Content-Type: text/plain; charset=UTF-8 5, 24 -- Content-Transfer-Encoding: 8bit 5, 24 -- Content-Disposition: inline ==============================End of - Headers==============================
I saw your post here and I don't know whether the TEM can punch a hole in the Si3N4 membrane or not. I've never had the opportunity to use such a grid. I do know that some materials can be made to react on the electron beam so for the sake of argument, let's say you can do it. Now, I recently have a lot of spare time on my hands and thought that I would answer your question with a few back-of-the-envelope calculations just for fun.
OK, there are three thicknesses of Si3N4 grids on the website that you gave, 20, 30, and 100 nm. (At the bottom of the page) and I'm going to consider two size holes, 2 nm and 5 nm, the two extremes in your question. How many molecules of Si3N4 are in those holes that are drilled that would be deposited onto the TEM holder and chamber walls? The density of Si3N4 is 3.1. The molecular weight is 140.28. How many atoms would stick on the walls and then how thick would it be? The following are my calculations.
What is the volume of material removed in nm^3? Consider a cylinder with the height the thickness of the film and the diameter the beam diameter. Volume of nm^3 Thickness 2nm diameter 5 nm diameter 20 62.8 392.7 30 94.2 589.0 100 314.2 1963.5
What is the volume of material in cm^3? Volume in cm^3 Thickness 2nm diameter 5 nm diameter 20 6.28E-20 3.93E-19 30 9.42E-20 5.89E-19 100 3.14E-19 1.96E-18
What is the weight of material in g removed? g of Si3N4 Thickness 2nm 5nm 20 1.95E-19 1.22E-18 30 2.92E-19 1.83E-18 100 9.74E-19 6.09E-18
How many molecules of Si3N4 would be removed? molecules of Si3N4 Thickness 2nm 5nm 20 8.36E+02 5.23E+03 30 1.25E+03 7.84E+03 100 4.18E+03 2.61E+04
How many atoms from the Si3N4 would be removed and stuck on walls? Consider perfect stoichiometry and perfect sticking. Simply multiply by 7. atoms from Si3N4 Thickness 2nm 5nm 20 5.85E+03 3.66E+04 30 8.78E+03 5.49E+04 100 2.93E+04 1.83E+05
Now let's assume that the area that the material will deposit on inside the TEM is a sphere with a diameter of 1 cm. The sample would be a the center of this sphere and the material would be deposited on the surface of the sphere. This would be the surface of everything such as pole pieces, sample holder, cold fingers, etc. This might be a little big, but not by much because it assumes and average distance of 5 mm to a surface from the sample. The area of that sphere is 3.14 cm^2.
How many atoms per square cm is deposited on that spherical surface assuming perfect stoichiometry and perfect sticking? atoms/cm2 Thickness 2nm 5nm 20 1.86E+03 1.16E+04 30 2.80E+03 1.75E+04 100 9.32E+03 5.82E+04
The average areal density of any surface is about 5 x 10^15 atoms per cm^2. How many holes would you have to drill to put one monolayer of atoms from the Si3N4 membrane on that surface assuming perfect stoichiometry and perfect sticking? # of Holes needed for a monolayer of Si3N4 Thickness 2nm 5nm 20 2.68E+12 4.29E+11 30 1.79E+12 2.86E+11 100 5.37E+11 8.59E+10
The short answer is that I think that you are safe if you drill a couple of holes.
-Scott
Scott D. Walck, Ph.D. Oceanside, CA 92056
S.Walck-at-cox.net SWalck65-at-gmail.com
-----Original Message----- X-from: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg] Sent: Tuesday, December 20, 2011 5:23 PM To: s.walck-at-cox.net
Dear All, a very good day to all ofyou! Recently a Professor would like to use the JEOL 2100F 's electron beam ( I am in charge of this TEM) to punch a 2-5nm hole on the SiN sample or perhaps his sample grid. I would like to ask your kind advices if there is any issue in letting him to do so such as if removing that amount of SiN might contaminate the chamber/column? His sample is bought from the commercial SiN grid (http://www.2spi.com/catalog/grids/silicon-nitride.php#6)
Cheers, Yee Yan NTU, MSE FACTS
==============================Original Headers============================== 2, 37 -- From one_twinklestar-at-yahoo.com.sg Tue Dec 20 19:05:11 2011 2, 37 -- Received: from nm23-vm1.bullet.mail.sg3.yahoo.com (nm23-vm1.bullet.mail.sg3.yahoo.com [106.10.151.64]) 2, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id pBL158ha024138 2, 37 -- for {microscopy-at-microscopy.com} ; Tue, 20 Dec 2011 19:05:10 -0600 2, 37 -- Received: from [106.10.166.125] by nm23.bullet.mail.sg3.yahoo.com with NNFMP; 21 Dec 2011 01:05:07 -0000 2, 37 -- Received: from [106.10.151.219] by tm14.bullet.mail.sg3.yahoo.com with NNFMP; 21 Dec 2011 01:05:07 -0000 2, 37 -- Received: from [127.0.0.1] by omp1017.mail.sg3.yahoo.com with NNFMP; 21 Dec 2011 01:05:07 -0000 2, 37 -- X-Yahoo-Newman-Property: ymail-3 2, 37 -- X-Yahoo-Newman-Id: 14172.40469.bm-at-omp1017.mail.sg3.yahoo.com 2, 37 -- Received: (qmail 87849 invoked by uid 60001); 21 Dec 2011 01:05:06 -0000 2, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com.sg; s=s1024; t=1324429506; bh=0KlYR8x2XHHoahovSKTQpkSJKHO07WxmWMcPHV0OI6Q=; h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIM E-Version:Content-Type:Content-Transfer-Encoding; b=nLhgrb4TBE/fiumKAbrg/bc1O6slK5PCqU1cCQJO2w/zwiZi/peuINmiSpUDnBYReBdOIISUfS /bgGi5AZn62O9zC4j6ozyCoxD7Lr9IZ2OgH59rmJA41X5A3FMSBfgkGSTk8Octxt3RcK34/s3OQf Z/Efc24xtPi6qqPglaoz8= 2, 37 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws; 2, 37 -- s=s1024; d=yahoo.com.sg; 2, 37 -- h=X-YMail-OSG:Received:X-Mailer:Message-ID:Date:From:Reply-To:Subject:To:MIM E-Version:Content-Type:Content-Transfer-Encoding; 2, 37 -- b=D6Cn7wz0X4MhOjM8f25utoCR6kebfh2LZgeFdPnwutRHuWZ32EOxWTZSJtzVp9CGq5VToA17n1 2aOfyp58yGFXwrb65YazwNJXHB5ANOtHiYRpMedRFsTRI9xq7EbwJ9Rw64CDrBCrkx9RN9rWkmBy ZTTSJISoN0BUBAIqwXva8=; 2, 37 -- X-YMail-OSG: Q34DfCgVM1mtYzvcPy20E5dZOI0rlFCylFAjLGdyLD6aQH. 2, 37 -- Nm7eG6AVt39BUyM9r8dBrcIWGZYg0xeeXEdlhcQgqXFGGeIxNSzMgzKCick. 2, 37 -- JKdUPwyereYFJTPe4Kzd7xkvH7yATrH7zIPQ.8pYP94WlBRRSqj.WDbtZofO 2, 37 -- veSIhPDBJzwJmSzzu0guuZIor37MWRsql33V3fGB75tM8p5PKwD2UDo.ifsw 2, 37 -- JS1ecvz_sHYMv2UhNYbusp0wwgkDwfct2iuneR3bL7GALuxVEMSxTf6j26Pk 2, 37 -- 13BLZcpYcm2SNdBknk.Dx7T8.LycfHrowz5gQCw_JZo_zvRDi7nd8pZpX7k5 2, 37 -- E0jM6_39TwgP8YfiRYmhyNaofd7lW_m9gOSfcNpRmIIJX7q_IAeVDDkqHLJx 2, 37 -- ZOYPINwPo0ZZwfdqpOyRdzPphJdkR9BcnV.mnIJOuO2OvdMHiRkEAdkkqDOO 2, 37 -- NeZBO48EaWWcu77GRI2fENy3mibA83tGEyHa5ZsFfdjug9PVR4RW5jYymn2A 2, 37 -- X 2, 37 -- Received: from [155.69.41.133] by web77402.mail.sg1.yahoo.com via HTTP; Wed, 21 Dec 2011 09:05:06 SGT 2, 37 -- X-Mailer: YahooMailWebService/0.8.115.331698 2, 37 -- Message-ID: {1324429506.87568.YahooMailNeo-at-web77402.mail.sg1.yahoo.com} 2, 37 -- Date: Wed, 21 Dec 2011 09:05:06 +0800 (SGT) 2, 37 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 2, 37 -- Reply-To: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg} 2, 37 -- Subject: About Punching a hole in SiN in TEM 2, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 2, 37 -- MIME-Version: 1.0 2, 37 -- Content-Type: text/plain; charset=iso-8859-1 2, 37 -- Content-Transfer-Encoding: 8bit 2, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBL158ha024138 ==============================End of - Headers==============================
==============================Original Headers============================== 26, 42 -- From s.walck-at-cox.net Wed Dec 21 17:25:22 2011 26, 42 -- Received: from fed1rmfepo103.cox.net (fed1rmfepo103.cox.net [68.230.241.145]) 26, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBLNPL7s025553 26, 42 -- for {microscopy-at-microscopy.com} ; Wed, 21 Dec 2011 17:25:21 -0600 26, 42 -- Received: from fed1rmimpo209.cox.net ([68.230.241.160]) 26, 42 -- by fed1rmfepo103.cox.net 26, 42 -- (InterMail vM.8.01.04.00 201-2260-137-20101110) with ESMTP 26, 42 -- id {20111221232520.LFQG3801.fed1rmfepo103.cox.net-at-fed1rmimpo209.cox.net} ; 26, 42 -- Wed, 21 Dec 2011 18:25:20 -0500 26, 42 -- Received: from WalckPC ([72.197.11.236]) 26, 42 -- by fed1rmimpo209.cox.net with bizsmtp 26, 42 -- id BzRJ1i00D55aDzc03zRJr4; Wed, 21 Dec 2011 18:25:18 -0500 26, 42 -- X-CT-Class: Clean 26, 42 -- X-CT-Score: 0.00 26, 42 -- X-CT-RefID: str=0001.0A020201.4EF26AE0.0071,ss=1,re=0.000,fgs=0 26, 42 -- X-CT-Spam: 0 26, 42 -- X-Authority-Analysis: v=1.1 cv=V7CHlOeuTX8qJ/M3k4aXqqkD6LmfniLgByWCSbrUMUU= 26, 42 -- c=1 sm=1 a=Vgsa08pI_fAA:10 a=G8Uczd0VNMoA:10 a=8nJEP1OIZ-IA:10 26, 42 -- a=s2s4rfcuBpqIM77q7Z6Q4A==:17 a=kviXuzpPAAAA:8 a=pGLkceISAAAA:8 26, 42 -- a=xvhTYyVHAAAA:8 a=Zx37jsudAAAA:8 a=KDoljRZ_AAAA:8 a=CjxXgO3LAAAA:8 26, 42 -- a=DJRV6DGia7knBcjlNcwA:9 a=FeL03cDVvoyQVkIrsRcA:7 a=wPNLvfGTeEIA:10 26, 42 -- a=-qaZg6oncsEA:10 a=RyjLG6jaqdoA:10 a=K3NO95Mr0sEA:10 a=Efwj4qSePvMA:10 26, 42 -- a=4vB-4DCPJfMA:10 a=MSl-tDqOz04A:10 a=G91thX30KR8A:10 26, 42 -- a=s2s4rfcuBpqIM77q7Z6Q4A==:117 26, 42 -- X-CM-Score: 0.00 26, 42 -- Authentication-Results: cox.net; auth=pass (LOGIN) smtp.auth=S.Walck-at-cox.net 26, 42 -- From: "Scott Walck" {S.Walck-at-cox.net} 26, 42 -- To: {microscopy-at-microscopy.com} 26, 42 -- Cc: {one_twinklestar-at-yahoo.com.sg} 26, 42 -- References: {201112210123.pBL1NFL3008557-at-ns.microscopy.com} 26, 42 -- In-Reply-To: {201112210123.pBL1NFL3008557-at-ns.microscopy.com} 26, 42 -- Subject: RE: [Microscopy] About Punching a hole in SiN in TEM 26, 42 -- Date: Wed, 21 Dec 2011 15:25:20 -0800 26, 42 -- Message-ID: {004701ccc037$ce7e26d0$6b7a7470$-at-cox.net} 26, 42 -- MIME-Version: 1.0 26, 42 -- Content-Type: text/plain; 26, 42 -- charset="iso-8859-1" 26, 42 -- X-Mailer: Microsoft Outlook 14.0 26, 42 -- Thread-Index: AQGzuCznC4Qxtwpbq9khBRcHtOtD9ZYY8c5Q 26, 42 -- Content-Language: en-us 26, 42 -- Content-Transfer-Encoding: 8bit 26, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBLNPL7s025553 ==============================End of - Headers==============================
Hello Everyone, Here's a link to a interesting article on light, microstructure and beetles. I'm a WIRED junky and enjoy this website very much. I hope you find the article on colors interesting.
Frank Karl Microscopist ARDL 2887 Gilchrist Road Akron, Ohio 44305 330-794-6600
________________________________ This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.
==============================Original Headers============================== 16, 25 -- From frank_karl-at-ardl.com Thu Dec 22 07:49:20 2011 16, 25 -- Received: from mail2.ardl.com (mail3.ardl.com [64.19.31.82]) 16, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBMDnKpP029442 16, 25 -- for {microscopy-at-microscopy.com} ; Thu, 22 Dec 2011 07:49:20 -0600 16, 25 -- Received: from exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0]) by 16, 25 -- exchange2k7.ad.ardl.com ([fe80::2c65:7444:ff02:1c0%12]) with mapi; Thu, 22 16, 25 -- Dec 2011 08:49:19 -0500 16, 25 -- From: Frank Karl {frank_karl-at-ardl.com} 16, 25 -- To: "Microscopy Listserver (E-mail)" {microscopy-at-microscopy.com} 16, 25 -- Disposition-Notification-To: Frank Karl {frank_karl-at-ardl.com} 16, 25 -- Return-Receipt-To: {frank_karl-at-ardl.com} 16, 25 -- Date: Thu, 22 Dec 2011 08:49:18 -0500 16, 25 -- Subject: interesting article on microscopy of colors 16, 25 -- Thread-Topic: interesting article on microscopy of colors 16, 25 -- Thread-Index: AczAsIBXkEATqDIzSqe+Syxf6EQ6gQ== 16, 25 -- Message-ID: {DB672743AFB6A64B9EB5CAC80AF1507701150229E9-at-exchange2k7.ad.ardl.com} 16, 25 -- Accept-Language: en-US 16, 25 -- Content-Language: en-US 16, 25 -- X-MS-Has-Attach: 16, 25 -- X-MS-TNEF-Correlator: 16, 25 -- acceptlanguage: en-US 16, 25 -- Content-Type: text/plain; charset="iso-8859-1" 16, 25 -- MIME-Version: 1.0 16, 25 -- Content-Transfer-Encoding: 8bit 16, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBMDnKpP029442 ==============================End of - Headers==============================
Make sure that the Aperture Diaphragm underneath the condenser is completely open!! Olympus' design of this piece is pretty thoughtless in this regard, as it is decoupled from the rotation of the condenser inserts... Also make sure that the condenser in setup for Köhler illumination before you try to image anything.
Good luck!!
Pete
On Fri, December 16, 2011 10:43 pm, microscopylistserver-noreply-at-microscopy.com wrote: | | | | ---------------------------------------------------------------------------- | The Microscopy ListServer -- CoSponsor: The Microscopy Society of America | To Subscribe/Unsubscribe -- | http://www.microscopy.com/MicroscopyListserver | On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html | ---------------------------------------------------------------------------- | | This Question/Comment was submitted to the Microscopy Listserver | using the WWW based Form at http://microscopy.com/MLFormMail.html | --------------------------------------------------------------------------- | Remember this posting is most likely not from a Subscriber, so when | replying please copy both | mlibbee-at-gmail.com as well as the MIcroscopy Listserver | --------------------------------------------------------------------------- | | Email: mlibbee-at-gmail.com Name: Marissa Mancuso | | Organization: LBL | | Title-Subject: [Filtered] Olympus BX 60 microcsope troubleshooting | | Message: Hello Listers | | I can't figure out why I am not able to image in darkfield mode with our | BX60 Olympus microscope. | I've followed the steps outlined in the manual a few times and think that | the 'cube selector' or | Brightfield/Darkfield Vertical Illuminator Attachment is out of alignment. | During the final step, I | can briefly see the image of the DF insert on the sample: central light is | blocked and the oblique | angles of light pass quickly over the surface of the sample. If anyone | has attempted to | correct/align the mirror cube location for proper light path selection, | will you please contact me | about your recommendations and experience? | | Thanks | marissa | | Login Host: 131.243.3.233 | --------------------------------------------------------------------------- | | | | ==============================Original | Headers============================== | 10, 25 -- From microscopylistserver-noreply-at-microscopy.com Fri Dec 16 | 15:22:20 2011 | 10, 25 -- Received: from znl.com ([206.69.208.20]) | 10, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id | pBGLMKJs009728 | 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:22:20 | -0600 | 10, 25 -- Received: from localhost (localhost [127.0.0.1]) | 10, 25 -- by znl.com (Postfix) with ESMTP id 3465F4590DE | 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:22:20 | -0600 (CST) | 10, 25 -- X-Virus-Scanned: amavisd-new at localhost.localdomain | 10, 25 -- Received: from znl.com ([127.0.0.1]) | 10, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, | port 10024) | 10, 25 -- with ESMTP id TdlwX-C2Ojna for {microscopy-at-microscopy.com} ; | 10, 25 -- Fri, 16 Dec 2011 15:22:19 -0600 (CST) | 10, 25 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com | [206.69.208.22]) | 10, 25 -- by znl.com (Postfix) with ESMTPA id 82D364590D3 | 10, 25 -- for {microscopy-at-microscopy.com} ; Fri, 16 Dec 2011 15:22:19 | -0600 (CST) | 10, 25 -- Message-ID: {4EEBB68B.6040502-at-microscopy.com} | 10, 25 -- Date: Fri, 16 Dec 2011 15:22:19 -0600 | 10, 25 -- From: MicroscopyListserver-NoReply | {microscopylistserver-noreply-at-microscopy.com} | 10, 25 -- Reply-To: mlibbee-at-gmail.com | 10, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; | en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 | 10, 25 -- MIME-Version: 1.0 | 10, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} | 10, 25 -- Subject: [Filtered] viaWWW:Olympus BX 60 microcsope troubleshooting | 10, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed | 10, 25 -- Content-Transfer-Encoding: 7bit | ==============================End of - | Headers============================== |
-- Peter Gabriel Pitrone - TechRMS Microscopy/Imaging Specialist Prof. Dr. Pavel Tomancak group Max Planck Institute for Molecular Biology and Genetics Pfotenhauerstr. 108 01307 Dresden
"If a straight line fit is required, obtain only two data points." - Anon.
==============================Original Headers============================== 12, 27 -- From microscopylistserver-noreply-at-microscopy.com Tue Dec 27 04:47:51 2011 12, 27 -- Received: from znl.com ([206.69.208.20]) 12, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBRAlp9J026457 12, 27 -- for {microscopy-at-microscopy.com} ; Tue, 27 Dec 2011 04:47:51 -0600 12, 27 -- Received: from localhost (localhost [127.0.0.1]) 12, 27 -- by znl.com (Postfix) with ESMTP id 33D4C46668E 12, 27 -- for {microscopy-at-microscopy.com} ; Tue, 27 Dec 2011 04:47:51 -0600 (CST) 12, 27 -- X-Virus-Scanned: amavisd-new at localhost.localdomain 12, 27 -- Received: from znl.com ([127.0.0.1]) 12, 27 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new, port 10024) 12, 27 -- with ESMTP id 3ir8Nvc1zMqL for {microscopy-at-microscopy.com} ; 12, 27 -- Tue, 27 Dec 2011 04:47:42 -0600 (CST) 12, 27 -- Received: from mac22.zaluzec.com (mac22.zaluzec.com [206.69.208.22]) 12, 27 -- by znl.com (Postfix) with ESMTPA id 31C5946667E 12, 27 -- for {microscopy-at-microscopy.com} ; Tue, 27 Dec 2011 04:47:42 -0600 (CST) 12, 27 -- Message-ID: {4EF9A24D.7000503-at-microscopy.com} 12, 27 -- Date: Tue, 27 Dec 2011 04:47:41 -0600 12, 27 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com} 12, 27 -- Reply-To: Peter Gabriel Pitrone {pitrone-at-mpi-cbg.de} 12, 27 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 12, 27 -- MIME-Version: 1.0 12, 27 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 27 -- Subject: [Filtered] Re: [Microscopy] viaWWW:Olympus BX 60 microcsope troubleshooting 12, 27 -- References: {201112162143.pBGLh9Uc002814-at-ns.microscopy.com} 12, 27 -- In-Reply-To: {201112162143.pBGLh9Uc002814-at-ns.microscopy.com} 12, 27 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed 12, 27 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
I was just reading over Beth's protocol and feel compelled to point out two errors so they won't get propagated. She suggests making two 1 M stocks of KH2PO4 and K2HPO4. That seems correct to me. She then says to mix 1.6 ml of one stock and 8.4 mls of the other and dilute to 100 mls with water. I assume the proportions are correct for the desired pH but at this point, one has a 0.1 M stock. When you then add 50 mls of the stock to 450 mls, you do a second 1 + 9 dilution and the phosphate buffer is now 0.01 M (10 mM) and not 500 ml of 0.1 M buffer as the protocol states. Furthermore, the protocol calls for 14.61 g of NaCl in 500 mls final volume. The MW of NaCl is 58.44 so the final concentration of NaCl is 0.25 M (250 mM) and not 500 mM as the protocol states. You may think you are using a high salt concentration but, in fact, it is on the low side of normal isotonicity. I don't mean to pile on here but there is another thing in the protocol that is a widely used convention that should be relegated to the dark ages of alchemy. As a general rule it is not very useful to refer to dilutions of antibodies when the true concentration is often known these days. Almost all monoclonals one purchases these days are at defined concentration. Even many polyclonals are affinity purified. I haven't used a secondary antibody of unknown concentration in over 15 years except for gold particles. Antibody concentrations should ideally be reported as concentrations (micrograms per ml). Generally a concentration of 1-10 ug/ml is considered correct. With gold-labeled secondaries one is still forced to give a dilution but the OD of the starting stock should be given or the number of particles/ml. Centrifuging gold particles must be done very cautiously and the time and g force should be given; the correct parameters are highly dependent on the particle size. Normally when one centrifuges antibodies, the idea is to spin the heck out of solutions and throw away any antibody aggregates that pellet down. With gold particles, th! e only r ationale for centrifuging would be to pellet the "good stuff" and discard any free antibody remaining in the supernatant and then re-suspend the pellet. I will defer to Jan Leunissen about whether that is a good idea or not. Sorry to be so critical but I have been a professor teaching undergrads too long to let this slip by. Happy New Year. Tom Phillips
Thomas E. Phillips, Ph.D Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) phillipst-at-missouri.edu
-----Original Message----- X-from: beth-at-plantbio.uga.edu [mailto:beth-at-plantbio.uga.edu] Sent: Friday, December 02, 2011 8:53 AM To: Phillips, Thomas E.
HI Monica, Below is our basic procedure for gold-labeling. Like Jan said the NaCl concentration matters. We tend to use a high salt content in the buffer. good luck with your labeling, Beth
GOLD LABELING PROTOCOL Sections must be on gold or gold-gilded grids.
Use a petri dish to create a moist chamber - wet filter paper with H2O (to keep moisture level high) then put a piece of parafilm on top of the moist filter paper. Do controls in a separate petri dish.
1. Place grid on a drop of KPBS (potassium phosphate buffered saline, 0.1M KPB and 0.5M NaCl, pH 7.2) - hydrate 5-10 minutes.
2. Next drop - 3% skim milk ((0.03g non-fat milk in 1 ml buffer), for 1 hour. Prepared fresh and centrifuged for a few seconds before use.
3. Next drop - KPBS - 5 minutes.
4. 1° Ab - dilution of your choice (usually 1:20) - 1 hour or more (centrifuge briefly before use). For a control = OMIT the primary antibody.
5. Rinse in buffer (use squeeze bottle), remove excess H2O with a filter paper.
6. 2° Ab - dilution of your choice (usually 1:50), 1 hr (centrifuge briefly before use).
7. Rinse for 30 sec in buffer (use squeeze bottle).
8. Rinse for 30 sec in dH2O (use squeeze bottle).
9. Post stain with uranyl acetate and lead citrate.
Variation: Use less salt in the buffer or different blocking agent or longer incubation times or different dilutions of primary and/or secondary antibodies.
Make Stock Buffer:
A) Potassium phosphate mono (KH2PO4) 1M - 6.8 g/50 ml ddH2O.
B) Potassium phosphate dibasic (K2HPO4) 1M - 8.71g/50 ml ddH2O
Use 1.6 ml of A + 8.4 ml of B = 10 ml Then add 90 ml dd H2O = 100 ml of stock buffer.
Put 14.61 g of NaCl in 450 ml of dd H2O* and stir. Then add 50 ml of stock buffer = 500 ml of 0.1 M KPBS in 0.5 M NaCl and stir.
==============================Original Headers============================== 19, 21 -- From beth-at-plantbio.uga.edu Fri Dec 2 08:52:04 2011 19, 21 -- Received: from dogwood.plantbio.uga.edu (pbmail.plantbio.uga.edu [128.192.26.3]) 19, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pB2Eq4t9006698 19, 21 -- for {microscopy-at-microscopy.com} ; Fri, 2 Dec 2011 08:52:04 -0600 19, 21 -- X-Footer: cGxhbnRiaW8udWdhLmVkdQ== 19, 21 -- Received: from [128.192.26.69] ([128.192.26.69]) 19, 21 -- (authenticated user beth-at-plantbio.uga.edu) 19, 21 -- by dogwood.plantbio.uga.edu 19, 21 -- (using TLSv1/SSLv3 with cipher AES128-SHA (128 bits)) 19, 21 -- for microscopy-at-microscopy.com; 19, 21 -- Fri, 2 Dec 2011 09:52:04 -0500 19, 21 -- Message-Id: {864C0DAC-06C5-4F88-9F03-ED2DE75E47B8-at-plantbio.uga.edu} 19, 21 -- From: Beth Richardson {beth-at-plantbio.uga.edu} 19, 21 -- To: microscopy microscopy {microscopy-at-microscopy.com} 19, 21 -- Content-Type: text/plain; charset=WINDOWS-1252; format=flowed; delsp=yes 19, 21 -- Mime-Version: 1.0 (Apple Message framework v936) 19, 21 -- Subject: immunogold labeling of chitosan 19, 21 -- Date: Fri, 2 Dec 2011 09:52:03 -0500 19, 21 -- X-Mailer: Apple Mail (2.936) 19, 21 -- Content-Transfer-Encoding: 8bit 19, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pB2Eq4t9006698 ==============================End of - Headers==============================
==============================Original Headers============================== 30, 33 -- From PhillipsT-at-missouri.edu Sat Dec 31 11:28:02 2011 30, 33 -- Received: from mxtip01-umsystem-out.um.umsystem.edu (mxtip01-umsystem-out.um.umsystem.edu [209.106.229.49]) 30, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id pBVHS2mF026760 30, 33 -- for {microscopy-at-microscopy.com} ; Sat, 31 Dec 2011 11:28:02 -0600 30, 33 -- X-IronPort-Anti-Spam-Filtered: true 30, 33 -- X-IronPort-Anti-Spam-Result: AoYAANxF/07PoJ7E/2dsb2JhbABAAw6cCo9AfIEFgXIBAQEETjcCAgIBCBEDAQEBCwIbBxYbARQJCAIEAQkJCBMCBIdhqxeJBQSIbII8YwSIBJ57Nw 30, 33 -- Received: from um-tcas1.um.umsystem.edu ([207.160.158.196]) 30, 33 -- by mxtip01-mizzou-out.um.umsystem.edu with ESMTP; 31 Dec 2011 11:28:01 -0600 30, 33 -- Received: from UM-MBX-T02.um.umsystem.edu ([169.254.2.191]) by 30, 33 -- UM-TCAS1.um.umsystem.edu ([207.160.158.196]) with mapi id 14.01.0355.002; 30, 33 -- Sat, 31 Dec 2011 11:28:01 -0600 30, 33 -- From: "Phillips, Thomas E." {PhillipsT-at-missouri.edu} 30, 33 -- To: "beth-at-plantbio.uga.edu" {beth-at-plantbio.uga.edu} , 30, 33 -- "leunissen-at-aurion.nl" 30, 33 -- {leunissen-at-aurion.nl} , 30, 33 -- "monica.nelea-at-polymtl.ca" {monica.nelea-at-polymtl.ca} , 30, 33 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 30, 33 -- Subject: RE: [Microscopy] immunogold labeling of chitosan 30, 33 -- Thread-Topic: [Microscopy] immunogold labeling of chitosan 30, 33 -- Thread-Index: AQHMsQIeC7rBqzbqlU2SKmFi+5NgWpX2WWrg 30, 33 -- Date: Sat, 31 Dec 2011 17:28:01 +0000 30, 33 -- Message-ID: {CB463A8DE3499A4CA204087E4EEB363D1235C878-at-UM-MBX-T02.um.umsystem.edu} 30, 33 -- References: {201112021453.pB2Er4KE007634-at-ns.microscopy.com} 30, 33 -- In-Reply-To: {201112021453.pB2Er4KE007634-at-ns.microscopy.com} 30, 33 -- Accept-Language: en-US 30, 33 -- Content-Language: en-US 30, 33 -- X-MS-Has-Attach: 30, 33 -- X-MS-TNEF-Correlator: 30, 33 -- x-originating-ip: [207.160.158.193] 30, 33 -- Content-Type: text/plain; charset="iso-8859-1" 30, 33 -- MIME-Version: 1.0 30, 33 -- Content-Transfer-Encoding: 8bit 30, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id pBVHS2mF026760 ==============================End of - Headers==============================
MicroscopyListserver Archive Email Extraction Software Version NJZ07060908