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From: zaluzec-at-aaem.amc.anl.gov
Date: Sat, 1 Jan 2011 09:04:45 -0600
Subject: [Microscopy] Administrivia: Happy New Year 2011

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the 19th year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

It was productive year for all of us. During 2010, the ListServer
delivered 1926 messages to over 3000 subscribers around the world,
with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated 460+ Gb of Email traffic and over
5.8 Million Email messages were sent out this year by my tired little server.
You don't want to know how much Junk Mail and spam has been filtered out.


The complete Microscopy ListServer Archives for 2010-1993 (~ are on-line at

http://www.microscopy.com.

A couple of final reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
your Email address from the listserver. The out-of-office / on-vacation
autoreply messages are a real nuisance to posters.

As always if you have questions about suitability of postings or
are having problems, feel free to contact me at (zaluzec-at-microscopy.com)

Cheers,

Nestor
Your Friendly Neighborhood SysOp


==============================Original Headers==============================
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12, 22 -- From: "Nestor J. Zaluzec" {zaluzec-at-aaem.amc.anl.gov}
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From: mike.bode-at-resaltatech.com
Date: Sat, 1 Jan 2011 12:45:21 -0600
Subject: [Microscopy] Administrivia: Happy New Year 2011

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


-----Original Message-----
X-from: Confocal Microscopy List [mailto:CONFOCALMICROSCOPY-at-LISTS.UMN.EDU] On Behalf Of Teng-Leong Chew
Sent: Saturday, January 01, 2011 3:16 AM
To: CONFOCALMICROSCOPY-at-LISTS.UMN.EDU

Thank you, Nestor, and a Happy New Year 2011 to you, too.

My I suggest that all participants at this list server silently give Nestor
a big hand for keeping it alive and a valuable resource to everybody (while
keeping the spam to a minimum)?

Thanks, Nestor.

Mike
---
Mike Bode, Ph.D.
ResAlta Research Technologies Corp.

2102 Beech Ct
Golden, CO 80401
USA

p: +1-303-748-4346
f: +1-303-202-6350
Mike.Bode-at-ResAltaTech.com
www.ResAltaTech.com





-----Original Message-----
X-from: zaluzec-at-aaem.amc.anl.gov [mailto:zaluzec-at-aaem.amc.anl.gov]
Sent: Saturday, January 01, 2011 8:15 AM
To: mike.bode-at-resaltatech.com

Happy New Year Colleagues;

Welcome to the 19th year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

It was productive year for all of us. During 2010, the ListServer
delivered 1926 messages to over 3000 subscribers around the world,
with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated 460+ Gb of Email traffic and
over
5.8 Million Email messages were sent out this year by my tired little
server.
You don't want to know how much Junk Mail and spam has been filtered out.


The complete Microscopy ListServer Archives for 2010-1993 (~ are on-line
at

http://www.microscopy.com.

A couple of final reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
your Email address from the listserver. The out-of-office / on-vacation
autoreply messages are a real nuisance to posters.

As always if you have questions about suitability of postings or
are having problems, feel free to contact me at (zaluzec-at-microscopy.com)

Cheers,

Nestor
Your Friendly Neighborhood SysOp


==============================Original Headers==============================
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From: kraftpiano-at-gmail.com
Date: Mon, 3 Jan 2011 14:34:43 -0600
Subject: [Microscopy] Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings everyone, and happy new year!

I was just working with a MetroCal (I think that's how it's spelled)
calibration wafer in an SEM, and I noticed that there is quite a bit
of dust on it. Is there a safe and effective way to clean a printed
silicon wafer which won't do any damage to it?

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
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4, 30 -- Subject: Cleaning silicon wafers
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From: kenconverse-at-qualityimages.biz
Date: Mon, 3 Jan 2011 16:41:57 -0600
Subject: [Microscopy] Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,
I looked on Metrocal's website and didn't find anything on silicon. Is it
perhaps from Geller? Anyway, if you can find the actual manufacturer, there
should be cleaning instructions available online.

If not, items on silicon wafers are actually pretty hardy if you don't
physically scratch them. A duster, for starters, may take care of the dust.
Is it mounted on a stub? Do you know what it's mounted with? Organic
solvents (alcohols, acetone) shouldn't hurt the silicon or metallization
(usually Al, sometimes Au) followed by a duster. The mounting material is
more likely to present a problem than the wafer itself.

Ken Converse
owner


QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Monday, January 03, 2011 3:38 PM
To: kenconverse-at-qualityimages.biz

Greetings everyone, and happy new year!

I was just working with a MetroCal (I think that's how it's spelled)
calibration wafer in an SEM, and I noticed that there is quite a bit
of dust on it. Is there a safe and effective way to clean a printed
silicon wafer which won't do any damage to it?

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

==============================Original Headers==============================
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4, 30 -- Subject: Cleaning silicon wafers
4, 30 -- From: Justin Kraft {kraftpiano-at-gmail.com}
4, 30 -- To: microscopy-at-microscopy.com
4, 30 -- Content-Type: text/plain; charset=ISO-8859-1
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==============================Original Headers==============================
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From: pitrone-at-mpi-cbg.de
Date: Mon, 3 Jan 2011 18:33:15 -0600
Subject: [Microscopy] viaWWW: REMINDER: Microscopist/Imaging-specialist position/s open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both pitrone-at-mpi-cbg.de as well as the MIcroscopy Listserver
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Email: pitrone-at-mpi-cbg.de
Name: Peter Pitrone

Organization: Max Planck Institute for Molecular Cell Biology and Genetics

Title-Subject: [Filtered] REMINDER:
Microscopist/Imaging-specialist position/s open
immediately: MPI-CBG Light Microscopy Facility in
Dresden, Germany

Message: Dear friends of microscopy and imaging,

We are very actively searching for 2
microscopists / imaging specialists to join our
light microscopy facility team with immediate
effect.

see:
http://www.mpi-cbg.de/jobs/jobs-at-the-mpi-cbg/microscopistsimaging-specialists.html

In case of any questions, please feel free to contact us: peychl-at-mpi-cbg.de

Please pass on the advert to anyone who you think might be interested.

Good luck to any and all who apply!!

Peter Pitrone

------------------------------------------------------------

Full Text of advert follows:

The Max Planck Institute of Molecular Cell
Biology and Genetics (MPI-CBG) in Dresden is
seeking motivated
Microscopists/Imaging Specialists, to work in its
Light Microscopy Facility (LMF).

The LMF is a large core facility enabling
different aspects of MPI-CBG researcherŪs imaging
projects including: design of imaging
experiments, choice of and training on suitable
imaging systems, and image processing,
visualisation and analysis. The LMF is part of a
network of core imaging facilities on the Dresden
Biopolis campus (see https://ifn.mpi-cbg.de.)
Light microscopy is one of the key methods of the
MPI-CBG. The LMF provides more than 20 advanced
imaging systems including laser scanning confocal
microscopy, two-photon microscopy, wide-field
microscopy, TIRF, SPIM and PALM/STORM. A super
resolution structured illumination (SIM) system
will be installed in 2011. The LMF team supports
approximately 270 users from over 35 countries.
More than 40 000 hours of instrument time is
booked annually. The working language of the
MPI-CBG is English.

Requirements:
We are seeking 1-2 candidates who are proactive
and service-oriented team players with excellent
interpersonal and organizational skills and who
enjoy helping people. They should have a first
degree (B.Sc.) and preferably also a doctorate
degree (Ph.D.) in biology or physics/optics, be
fluent in English and be ready to help scientists
with both trivial and advanced aspects of light
microscopy imaging projects. Experience in basic
as well as advanced light microscopy is required
and some experience with image analysis would be
a strong plus. The successful candidate(s) should
be ready to serve in a dynamic international
multi-user environment whereby four basic areas
of service must be covered: a) scientific support
of imaging projects, b) implementation of new
imaging technologies, c) maintenance and service
of current imaging systems and d) training and
teaching light microscopy. Ideally, the
candidate(s) should have experience working in an
imaging facility.

The positions are available immediately. The
initial contract is for 2 years. Compensation
will depend on the qualifications and experience
of the candidate. The Max Planck Society is
committed to employ more disabled persons. The
application of disabled persons is strongly
encouraged. The Max Planck Society is committed
to increase the number of female scientists.
Women are particularly encouraged to apply.

For informal inquiries, please contact the leader
of the LMF, Dr. Jan Peychl, email:
peychl-at-mpi-cbg.de

If you wish to apply for this position, please
send your CV, letter of application/motivation
and the contact information of three referees to:

Max Planck Institute
of Molecular Cell Biology and Genetics
Code: 2010-LMF-4100
Pfotenhauerstr. 108
01307 Dresden
Germany
or by email: 2010-LMF-4100-at-mpi-cbg.de

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From: DUNHAMDJ-at-uwec.edu
Date: Tue, 4 Jan 2011 11:24:05 -0600
Subject: [Microscopy] Available Position: Analytical Scientist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Analytical Scientist

The University of Wisconsin Eau Claire is seeking applicants for an Analytical Scientist position in the Materials Science Center, an interdisciplinary analytical facility, specializing in materials characterization.† This is a full-time professional academic staff position beginning July 1st 2011.† Potential applicants may obtain a complete position description and application requirements at http://www.uwec.edu/Matsci/position.html. Women, minorities, individuals with disabilities and veterans are encouraged to apply.† Criminal background checks are required prior to employment.† Review of completed applications will commence January 14th, 2011 and continue until the position is filled.

The scientist will help manage instrumentation associated with the Materials Science Center, an interdisciplinary analytical facility that incorporates an array of state-of-the-art instrumentation specializing in materials characterization and surface science.† Instrumentation within the Center includes a 200 keV Transmission Electron Microscope, Scanning Electron Microscopes with Energy Dispersive X-ray Microanalyzer, a High Resolution Inductively Coupled Plasma - Mass Spectrometer, X-ray Photoelectron Spectrometer, Scanning Tunneling Microscope, 2 X-ray Fluorescence Spectrometers, X-ray Diffractometer, Atomic Force Microscopes, Scanning Auger Nanoprobe and a Micro-FTIR.† The scientist will assist with the development of the Electron Microscopy facility, and will be involved in all aspects of technique development, instrument control and maintenance, and other aspects of running a professional laboratory.† The scientist will be responsible for instrument maintenance, repair, sample preparation and analysis in other high vacuum applications, such as XRD, XRF, XPS and STM utilized by faculty associated with the Materials Science Center.† Precise responsibilities will vary according to research priorities within the Materials Science Center.† The scientist is encouraged to participate in collaborative research with faculty, undergraduates and local industry, as well as other scholarly activities.† The scientist may be asked to teach some instrument specific courses.


-----------------------------------------------------------------------------------
Dr. Doug Dunham
Director, Materials Science Center
University of Wisconsin-Eau Claire
Phillips Hall, Suite 177
105 Garfield Avenue
Eau Claire, WI 54702-4004
tel:† 715-836-5312†† fax:† 715-836-3556



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From: aheath-at-ufl.edu
Date: Tue, 4 Jan 2011 19:13:25 -0600
Subject: [Microscopy] viaWWW: SEM-- Quantomix Wet SEM capsules?

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Email: aheath-at-ufl.edu
Name: Ann Heatherington

Organization: Dept. of Geological Sciences, University of Florida

Title-Subject: [Filtered] SEM-- Quantomix Wet SEM capsules?

Message: Hello-- Does anyone have any experience with the Wet SEM
capsules by Quantomix?


We have a Zeiss EVO MA 10 SEM and every once in a while have someone
interested in looking at a wet sample (gels, sediments, etc.) This
looked like a possible economical way of gaining wet sample analysis
capability, and I would like to hear opinions from someone who has
actually used it. The vendor will not supply a customer list.


Thank you,
Ann Heatherington


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From: kintzjd-at-rocketmail.com
Date: Tue, 4 Jan 2011 19:14:08 -0600
Subject: [Microscopy] viaWWW: Repair or replace EDS detector?

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Email: kintzjd-at-rocketmail.com
Name: Joe Kintz

Organization: Stress Engineering Services, Inc.

Title-Subject: [Filtered] Repair or replace EDS detector?

Message: Our EDAX-brand Sapphire Si(Li) EDS detector probably has a
crack or hole in its super ultra thin window and will probably have
to be sent to EDAX for repair. Is this a good time to consider
upgrading to a silicon drift detector? Using liquid nitrogen is not
a problem for us but, if we can improve EDS performance at a
reasonable price compared to repairing our existing Si(Li) detector,
this would seem to make sense. We're also using EDAX's EBSD system,
so we'd like to continue using their Genesis EDS software.

If this would be a good time to upgrade, what is the concensus about
the silicon drift detectors (SDD)? Are they better across the board
than the Si(Li) detectors, or would we be giving up some aspect of
performance? Who makes the best SDD's that will work with EDAX EDS
systems?

Thanks in advance for your assistance.

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From: michael.anderson-at-nist.gov
Date: Wed, 5 Jan 2011 09:17:09 -0600
Subject: [Microscopy] Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
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Justin,

I would agree with Ken on the use of a compressed gas as a first attempt at cleaning your wafer, but I would actually avoid those tetrafluoroethane dusters. It's been my experience that they can leave a film on the surface of the wafer. If you have a spare piece of polished silicon wafer hanging around you could test the duster on it to see if it leaves a film. I prefer using high purity dry nitrogen from a gas cylinder, but I have also used the compressed CO2 dusters you can find at office supply stores with good results.

Since it looks like this wafer is etched silicon, you should be safe using a solvent if there is anything stuck on it, but I would stress that you should contact the manufacturer (Metroboost*) to make sure that they don't have some kind of a coating on it or something that would be damaged by a particular solvent. A good general choice to start would actually be deionized water, since the etch process to make the wafer probably employed that anyway. The procedure that we used during my semiconductor courses was to rinse the chip or wafer with DI water and then blow-dry it with dry nitrogen. It helps if you hold the wafer near vertical with one end resting on a lint-free paper towel and then blow the residual solvent towards the towel starting from the top of the wafer and working your way down. This generally leaves a clean, lint free, surface.

Hope that helps!

Mike Anderson

*Comments made in this correspondence represent the opinion of the author and should not be construed as endorsement or recommendation by NIST. Likewise, any mention of commercial products does not imply recommendation or endorsement by NIST.


-----Original Message-----
X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz]
Sent: Monday, January 03, 2011 5:51 PM
To: Anderson, Michael

Justin,
I looked on Metrocal's website and didn't find anything on silicon. Is it
perhaps from Geller? Anyway, if you can find the actual manufacturer, there
should be cleaning instructions available online.

If not, items on silicon wafers are actually pretty hardy if you don't
physically scratch them. A duster, for starters, may take care of the dust.
Is it mounted on a stub? Do you know what it's mounted with? Organic
solvents (alcohols, acetone) shouldn't hurt the silicon or metallization
(usually Al, sometimes Au) followed by a duster. The mounting material is
more likely to present a problem than the wafer itself.

Ken Converse
owner


QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
Sent: Monday, January 03, 2011 3:38 PM
To: kenconverse-at-qualityimages.biz

Greetings everyone, and happy new year!

I was just working with a MetroCal (I think that's how it's spelled)
calibration wafer in an SEM, and I noticed that there is quite a bit
of dust on it. Is there a safe and effective way to clean a printed
silicon wafer which won't do any damage to it?

--Justin A. Kraft

--
"America believes in education; the average professor earns more money
in a year than a professional athlete earns in a whole week." Evan
Esar

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30, 28 -- Subject: RE: [Microscopy] RE: Cleaning silicon wafers
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From: mcintoshgreece-at-yahoo.com
Date: Wed, 5 Jan 2011 13:44:50 -0600
Subject: [Microscopy] Use Tape: Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,

Google these topics for alternate ideas on removing contamination:

Extraction replica techniques (Cellulose Acetate)
Semiconductor Processing Tape ("Blue Tack tape")


http://www.lpi.usra.edu/meetings/lpsc2007/pdf/1920.pdf

http://www.semicorp.com/articles/sticky-issues-with-semiconductor-processing-tape.html


John McIntosh



--- On Wed, 1/5/11, michael.anderson-at-nist.gov {michael.anderson-at-nist.gov} wrote:

} From: michael.anderson-at-nist.gov {michael.anderson-at-nist.gov}
} Subject: [Microscopy] Cleaning silicon wafers
} To: mcintoshgreece-at-yahoo.com
} Date: Wednesday, January 5, 2011, 10:23 AM
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:† The
} Microscopy Society of America
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} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Justin,
}
} I would agree with Ken on the use of a compressed gas as a
} first attempt at cleaning your wafer, but I would actually
} avoid those tetrafluoroethane dusters.† It's been my
} experience that they can leave a film on the surface of the
} wafer.† If you have a spare piece of polished silicon
} wafer hanging around you could test the duster on it to see
} if it leaves a film.† I prefer using high purity dry
} nitrogen from a gas cylinder, but I have also used the
} compressed CO2 dusters you can find at office supply stores
} with good results.†
}
} Since it looks like this wafer is etched silicon, you
} should be safe using a solvent if there is anything stuck on
} it, but I would stress that you should contact the
} manufacturer (Metroboost*) to make sure that they don't have
} some kind of a coating on it or something that would be
} damaged by a particular solvent. A good general choice to
} start would actually be deionized water, since the etch
} process to make the wafer probably employed that
} anyway.† The procedure that we used during my
} semiconductor courses was to rinse the chip or wafer with DI
} water and then blow-dry it with dry nitrogen.† It helps
} if you hold the wafer near vertical with one end resting on
} a lint-free paper towel and then blow the residual solvent
} towards the towel starting from the top of the wafer and
} working your way down.† This generally leaves a clean,
} lint free, surface.
}
} Hope that helps!
}
} Mike Anderson

} Justin,
} I looked on Metrocal's website and didn't find anything on
} silicon.† Is it
} perhaps from Geller?† Anyway, if you can find the
} actual manufacturer, there
} should be cleaning instructions available online.
}
} If not, items on silicon wafers are actually pretty hardy
} if you don't
} physically scratch them.† A duster, for starters, may
} take care of the dust.
} Is it mounted on a stub?† Do you know what it's
} mounted with?† Organic
} solvents (alcohols, acetone) shouldn't hurt the silicon or
} metallization
} (usually Al, sometimes Au) followed by a duster.† The
} mounting material is
} more likely to present a problem than the wafer itself.
}
} Ken Converse
} owner
}
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME† 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}

} Greetings everyone, and happy new year!
}
} I was just working with a MetroCal (I think that's how it's
} spelled)
} calibration wafer in an SEM, and I noticed that there is
} quite a bit
} of dust on it.† Is there a safe and effective way to
} clean a printed
} silicon wafer which won't do any damage to it?
}
} --Justin A. Kraft
}





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From: shishkov-at-HELIX.MGH.HARVARD.EDU
Date: Wed, 5 Jan 2011 14:03:24 -0600
Subject: [Microscopy] RE: Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Check this product as well:
http://www.vatran.com/dryice.html

--
Milen Shishkov, Ph.D.
Wellman Center for Photomedicine
Massachusetts General Hospital
Harvard Medical School

Address: MGH
BAR 712
50 Blossom St.
Boston, MA 02114
Phone: (617) 726 1589
Fax: (617) 643 9208




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
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dispose of the e-mail.



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From: FMonson-at-wcupa.edu
Date: Wed, 5 Jan 2011 16:51:39 -0600
Subject: [Microscopy] RE: Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Why not try Critical Point Drying (CPD)??? Prices range from $5k (manual) to - heading toward - $100k for automation, cleanroom, big wafers.

URL: http://lib.semi.ac.cn:8080/tsh/dzzy/wsqk/SPIE/vol3880/3880-51.pdf

Check Tousimis: http://tousimis.com/critical_point_dryers/critical_point_dryer.html

We have a CPD from tousimis (815 AutoSamDri, Series A)

Pricey, but automatic and based on PhysChem. As tousimis makes big ones for complete mems wafers, plain silicon chips (i.e. 5 x 5mm) are not a problem. Biggest problem is, once retrieved in open lab, how does one protect the chip from instant contamination. My followup would be plasma cleaning to clear off any carbon deposited between CPD and final use.

If all were done in clean room or gloved environment, then CPD - despite cost - may be the way to go both before and after processing the wafer). I would strongly recommend enthusiastic consideration.

Cheers,

Fred Monson

P.S. I'm a biologist first, so I am poking my head into an other's place. Thus, I realize the value of advice may appear depreciated by background. Howsoever, please consider the fact that since we messy organic microscopic investigators spend our lives looking at artifact and gathering together to grant each other various levels of group reality for each and every microscopic finding, we are also those who strive most eagerly for the purity of mere 3D problems. To save an ultrastructural projection on the surface of a cell, there is NOTHING like perfect CPD.

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl


-----Original Message-----
X-from: shishkov-at-HELIX.MGH.HARVARD.EDU [mailto:shishkov-at-HELIX.MGH.HARVARD.EDU]
Sent: Wednesday, January 05, 2011 3:11 PM
To: Monson, Frederick

Check this product as well:
http://www.vatran.com/dryice.html

--
Milen Shishkov, Ph.D.
Wellman Center for Photomedicine
Massachusetts General Hospital
Harvard Medical School

Address: MGH
BAR 712
50 Blossom St.
Boston, MA 02114
Phone: (617) 726 1589
Fax: (617) 643 9208




The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.



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27, 29 -- Subject: RE: [Microscopy] RE: Cleaning silicon wafers
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From: r-holdford-at-ti.com
Date: Wed, 5 Jan 2011 17:49:40 -0600
Subject: [Microscopy] Re: Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'll just echo what Ken Converse and Mike Anderson have to say
and add my 2 cents' worth. I work with Si wafers (patterned and
unpatterned) everyday. I also have an SEM standard similar to
the one you are using. I usually clean mine (after rapping the
knuckles of the person who got it dirty) first with dry N2. If
you have some stubborn dust particles, a short sonication in warm
DI or distilled water should do it. One of those little brushes
that photographers used to use to get dust off negatives would be
good also, since they neutralize the static charges that make for
some tenacious particles. I have deionizers all over the place,
so I use those. After all the effort you put into cleaning the
standard, store it in as dust-free an atmosphere and container as
you have available. I store mine in the metal box it came in, in
a dry nitrogen cabinet.

If some ham-handed user has stuck their finger to it, that's more
difficult. CO2 snow is a wizard for removing organic
contaminants such as finger oils but not everybody has access to
a unit. In this case (if the stub attachment will stand it) I
would try reagent-grade (or fab-grade) acetone, using it in the
manner Mike suggests. I would follow this with some reagent- or
fab-grade methanol as sometimes acetone can leave a residue.

On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
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} ----------------------------------------------------------------------------
}
} Justin,
}
} I would agree with Ken on the use of a compressed gas as a first attempt at cleaning your wafer, but I would actually avoid those tetrafluoroethane dusters. It's been my experience that they can leave a film on the surface of the wafer. If you have a spare piece of polished silicon wafer hanging around you could test the duster on it to see if it leaves a film. I prefer using high purity dry nitrogen from a gas cylinder, but I have also used the compressed CO2 dusters you can find at office supply stores with good results.
}
} Since it looks like this wafer is etched silicon, you should be safe using a solvent if there is anything stuck on it, but I would stress that you should contact the manufacturer (Metroboost*) to make sure that they don't have some kind of a coating on it or something that would be damaged by a particular solvent. A good general choice to start would actually be deionized water, since the etch process to make the wafer probably employed that anyway. The procedure that we used during my semiconductor courses was to rinse the chip or wafer with DI water and then blow-dry it with dry nitrogen. It helps if you hold the wafer near vertical with one end resting on a lint-free paper towel and then blow the residual solvent towards the towel starting from the top of the wafer and working your way down. This generally leaves a clean, lint free, surface.
}
} Hope that helps!
}
} Mike Anderson
}
} *Comments made in this correspondence represent the opinion of the author and should not be construed as endorsement or recommendation by NIST. Likewise, any mention of commercial products does not imply recommendation or endorsement by NIST.
}
}
} -----Original Message-----
} X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz]
} Sent: Monday, January 03, 2011 5:51 PM
} To: Anderson, Michael
} Subject: [Microscopy] RE: Cleaning silicon wafers
}
}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Justin,
} I looked on Metrocal's website and didn't find anything on silicon. Is it
} perhaps from Geller? Anyway, if you can find the actual manufacturer, there
} should be cleaning instructions available online.
}
} If not, items on silicon wafers are actually pretty hardy if you don't
} physically scratch them. A duster, for starters, may take care of the dust.
} Is it mounted on a stub? Do you know what it's mounted with? Organic
} solvents (alcohols, acetone) shouldn't hurt the silicon or metallization
} (usually Al, sometimes Au) followed by a duster. The mounting material is
} more likely to present a problem than the wafer itself.
}
} Ken Converse
} owner
}
}
} QUALITY IMAGES
} Servicing Scanning Electron Microscopes
} Since 1981
} 474 So. Bridgton Rd.
} Bridgton, ME 04009
} 207-647-4348
} Fax 207-647-2688
} kenconverse-at-qualityimages.biz
} qualityimages.biz
}
}
} -----Original Message-----
} X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} Sent: Monday, January 03, 2011 3:38 PM
} To: kenconverse-at-qualityimages.biz
} Subject: [Microscopy] Cleaning silicon wafers
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Greetings everyone, and happy new year!
}
} I was just working with a MetroCal (I think that's how it's spelled)
} calibration wafer in an SEM, and I noticed that there is quite a bit
} of dust on it. Is there a safe and effective way to clean a printed
} silicon wafer which won't do any damage to it?
}
} --Justin A. Kraft
}

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
214-567-0360
SC Packaging FA
Texas Instruments, Inc.
13536 N. Central Expressway MS 940
Dallas, TX 75243
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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From: oshel1pe-at-cmich.edu
Date: Thu, 6 Jan 2011 07:09:46 -0600
Subject: [Microscopy] Re: Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

No special unit is needed for CO2 snow, just a tank of Clean! CO2.
Either remove any hose, or attach the tank hose to something weighty
- any block of metal with a threaded hole into which the hose can be
screwed, or the like. Something to hold the hose firmly so it doesn't
whip around. Turn on the tank, and the expanding CO2 makes snow. (I
used to do this into a pillow case for sending samples from the
Arctic.) This worked with both regular and siphon CO2 tanks.
Now, I use the exhaust outlet (not "Vent") of our Polaron bomb CPD if
I need snow. (Because of the weightiness of the CPD.)
I suggest food grade CO2, but an absolutely dry grade may be needed
for your application.

Phil

} I'll just echo what Ken Converse and Mike Anderson have to say
} and add my 2 cents' worth. I work with Si wafers (patterned and
} unpatterned) everyday. I also have an SEM standard similar to
} the one you are using. I usually clean mine (after rapping the
} knuckles of the person who got it dirty) first with dry N2. If
} you have some stubborn dust particles, a short sonication in warm
} DI or distilled water should do it. One of those little brushes
} that photographers used to use to get dust off negatives would be
} good also, since they neutralize the static charges that make for
} some tenacious particles. I have deionizers all over the place,
} so I use those. After all the effort you put into cleaning the
} standard, store it in as dust-free an atmosphere and container as
} you have available. I store mine in the metal box it came in, in
} a dry nitrogen cabinet.
}
} If some ham-handed user has stuck their finger to it, that's more
} difficult. CO2 snow is a wizard for removing organic
} contaminants such as finger oils but not everybody has access to
} a unit. In this case (if the stub attachment will stand it) I
} would try reagent-grade (or fab-grade) acetone, using it in the
} manner Mike suggests. I would follow this with some reagent- or
} fab-grade methanol as sometimes acetone can leave a residue.
}
} On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Justin,
} }
} } I would agree with Ken on the use of a compressed gas as a first
} } attempt at cleaning your wafer, but I would actually avoid those
} } tetrafluoroethane dusters. It's been my experience that they can
} } leave a film on the surface of the wafer. If you have a spare
} } piece of polished silicon wafer hanging around you could test the
} } duster on it to see if it leaves a film. I prefer using high
} } purity dry nitrogen from a gas cylinder, but I have also used the
} } compressed CO2 dusters you can find at office supply stores with
} } good results.
} }
} } Since it looks like this wafer is etched silicon, you should be
} } safe using a solvent if there is anything stuck on it, but I would
} } stress that you should contact the manufacturer (Metroboost*) to
} } make sure that they don't have some kind of a coating on it or
} } something that would be damaged by a particular solvent. A good
} } general choice to start would actually be deionized water, since
} } the etch process to make the wafer probably employed that anyway.
} } The procedure that we used during my semiconductor courses was to
} } rinse the chip or wafer with DI water and then blow-dry it with dry
} } nitrogen. It helps if you hold the wafer near vertical with one
} } end resting on a lint-free paper towel and then blow the residual
} } solvent towards the towel starting from the top of the wafer and
} } working your way down. This generally leaves a clean, lint free,
} } surface.
} }
} } Hope that helps!
} }
} } Mike Anderson
} }
} } *Comments made in this correspondence represent the opinion of the
} } author and should not be construed as endorsement or recommendation
} } by NIST. Likewise, any mention of commercial products does not
} } imply recommendation or endorsement by NIST.
} }
} }
} } -----Original Message-----
} } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz]
} } Sent: Monday, January 03, 2011 5:51 PM
} } To: Anderson, Michael
} } Subject: [Microscopy] RE: Cleaning silicon wafers
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Justin,
} } I looked on Metrocal's website and didn't find anything on silicon. Is it
} } perhaps from Geller? Anyway, if you can find the actual manufacturer, there
} } should be cleaning instructions available online.
} }
} } If not, items on silicon wafers are actually pretty hardy if you don't
} } physically scratch them. A duster, for starters, may take care of the dust.
} } Is it mounted on a stub? Do you know what it's mounted with? Organic
} } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization
} } (usually Al, sometimes Au) followed by a duster. The mounting material is
} } more likely to present a problem than the wafer itself.
} }
} } Ken Converse
} } owner
} }
} }
} } QUALITY IMAGES
} } Servicing Scanning Electron Microscopes
} } Since 1981
} } 474 So. Bridgton Rd.
} } Bridgton, ME 04009
} } 207-647-4348
} } Fax 207-647-2688
} } kenconverse-at-qualityimages.biz
} } qualityimages.biz
} }
} }
} } -----Original Message-----
} } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } Sent: Monday, January 03, 2011 3:38 PM
} } To: kenconverse-at-qualityimages.biz
} } Subject: [Microscopy] Cleaning silicon wafers

} } Greetings everyone, and happy new year!
} }
} } I was just working with a MetroCal (I think that's how it's spelled)
} } calibration wafer in an SEM, and I noticed that there is quite a bit
} } of dust on it. Is there a safe and effective way to clean a printed
} } silicon wafer which won't do any damage to it?
} }
} } --Justin A. Kraft
} }
}
} --
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Becky Holdford (r-holdford-at-ti.com)
} 214-567-0360
} SC Packaging FA
} Texas Instruments, Inc.
} 13536 N. Central Expressway MS 940
} Dallas, TX 75243
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
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From: r-holdford-at-ti.com
Date: Thu, 6 Jan 2011 14:10:41 -0600
Subject: [Microscopy] Re: Re: Cleaning silicon wafers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Phil: thanks bunches for this cool (pun intended) tip!

On 1/6/11 7:09 AM, oshel1pe-at-cmich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} No special unit is needed for CO2 snow, just a tank of Clean! CO2.
} Either remove any hose, or attach the tank hose to something weighty
} - any block of metal with a threaded hole into which the hose can be
} screwed, or the like. Something to hold the hose firmly so it doesn't
} whip around. Turn on the tank, and the expanding CO2 makes snow. (I
} used to do this into a pillow case for sending samples from the
} Arctic.) This worked with both regular and siphon CO2 tanks.
} Now, I use the exhaust outlet (not "Vent") of our Polaron bomb CPD if
} I need snow. (Because of the weightiness of the CPD.)
} I suggest food grade CO2, but an absolutely dry grade may be needed
} for your application.
}
} Phil
}
} } I'll just echo what Ken Converse and Mike Anderson have to say
} } and add my 2 cents' worth. I work with Si wafers (patterned and
} } unpatterned) everyday. I also have an SEM standard similar to
} } the one you are using. I usually clean mine (after rapping the
} } knuckles of the person who got it dirty) first with dry N2. If
} } you have some stubborn dust particles, a short sonication in warm
} } DI or distilled water should do it. One of those little brushes
} } that photographers used to use to get dust off negatives would be
} } good also, since they neutralize the static charges that make for
} } some tenacious particles. I have deionizers all over the place,
} } so I use those. After all the effort you put into cleaning the
} } standard, store it in as dust-free an atmosphere and container as
} } you have available. I store mine in the metal box it came in, in
} } a dry nitrogen cabinet.
} }
} } If some ham-handed user has stuck their finger to it, that's more
} } difficult. CO2 snow is a wizard for removing organic
} } contaminants such as finger oils but not everybody has access to
} } a unit. In this case (if the stub attachment will stand it) I
} } would try reagent-grade (or fab-grade) acetone, using it in the
} } manner Mike suggests. I would follow this with some reagent- or
} } fab-grade methanol as sometimes acetone can leave a residue.
} }
} } On 1/5/11 9:17 AM, michael.anderson-at-nist.gov wrote:
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Justin,
} } }
} } } I would agree with Ken on the use of a compressed gas as a first
} } } attempt at cleaning your wafer, but I would actually avoid those
} } } tetrafluoroethane dusters. It's been my experience that they can
} } } leave a film on the surface of the wafer. If you have a spare
} } } piece of polished silicon wafer hanging around you could test the
} } } duster on it to see if it leaves a film. I prefer using high
} } } purity dry nitrogen from a gas cylinder, but I have also used the
} } } compressed CO2 dusters you can find at office supply stores with
} } } good results.
} } }
} } } Since it looks like this wafer is etched silicon, you should be
} } } safe using a solvent if there is anything stuck on it, but I would
} } } stress that you should contact the manufacturer (Metroboost*) to
} } } make sure that they don't have some kind of a coating on it or
} } } something that would be damaged by a particular solvent. A good
} } } general choice to start would actually be deionized water, since
} } } the etch process to make the wafer probably employed that anyway.
} } } The procedure that we used during my semiconductor courses was to
} } } rinse the chip or wafer with DI water and then blow-dry it with dry
} } } nitrogen. It helps if you hold the wafer near vertical with one
} } } end resting on a lint-free paper towel and then blow the residual
} } } solvent towards the towel starting from the top of the wafer and
} } } working your way down. This generally leaves a clean, lint free,
} } } surface.
} } }
} } } Hope that helps!
} } }
} } } Mike Anderson
} } }
} } } *Comments made in this correspondence represent the opinion of the
} } } author and should not be construed as endorsement or recommendation
} } } by NIST. Likewise, any mention of commercial products does not
} } } imply recommendation or endorsement by NIST.
} } }
} } }
} } } -----Original Message-----
} } } X-from: kenconverse-at-qualityimages.biz [mailto:kenconverse-at-qualityimages.biz]
} } } Sent: Monday, January 03, 2011 5:51 PM
} } } To: Anderson, Michael
} } } Subject: [Microscopy] RE: Cleaning silicon wafers
} } }
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Justin,
} } } I looked on Metrocal's website and didn't find anything on silicon. Is it
} } } perhaps from Geller? Anyway, if you can find the actual manufacturer, there
} } } should be cleaning instructions available online.
} } }
} } } If not, items on silicon wafers are actually pretty hardy if you don't
} } } physically scratch them. A duster, for starters, may take care of the dust.
} } } Is it mounted on a stub? Do you know what it's mounted with? Organic
} } } solvents (alcohols, acetone) shouldn't hurt the silicon or metallization
} } } (usually Al, sometimes Au) followed by a duster. The mounting material is
} } } more likely to present a problem than the wafer itself.
} } }
} } } Ken Converse
} } } owner
} } }
} } }
} } } QUALITY IMAGES
} } } Servicing Scanning Electron Microscopes
} } } Since 1981
} } } 474 So. Bridgton Rd.
} } } Bridgton, ME 04009
} } } 207-647-4348
} } } Fax 207-647-2688
} } } kenconverse-at-qualityimages.biz
} } } qualityimages.biz
} } }
} } }
} } } -----Original Message-----
} } } X-from: kraftpiano-at-gmail.com [mailto:kraftpiano-at-gmail.com]
} } } Sent: Monday, January 03, 2011 3:38 PM
} } } To: kenconverse-at-qualityimages.biz
} } } Subject: [Microscopy] Cleaning silicon wafers
} } } Greetings everyone, and happy new year!
} } }
} } } I was just working with a MetroCal (I think that's how it's spelled)
} } } calibration wafer in an SEM, and I noticed that there is quite a bit
} } } of dust on it. Is there a safe and effective way to clean a printed
} } } silicon wafer which won't do any damage to it?
} } }
} } } --Justin A. Kraft
} } }
} } --
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} } Becky Holdford (r-holdford-at-ti.com)
} } 214-567-0360
} } SC Packaging FA
} } Texas Instruments, Inc.
} } 13536 N. Central Expressway MS 940
} } Dallas, TX 75243
} } ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

--
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Becky Holdford (r-holdford-at-ti.com)
214-567-0360
SC Packaging FA
Texas Instruments, Inc.
13536 N. Central Expressway MS 940
Dallas, TX 75243
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


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From: patpxs-at-gmail.com
Date: Thu, 6 Jan 2011 16:23:46 -0600
Subject: [Microscopy] Darkroom Equipment Free to Good Home

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

The EM Lab at Duke Hospital has gone digital so we have a couple of
darkroom goodies to give away.

Of course, you must pay for the shipping and handling but think of all
the money you'll save!

The items are:

Ilford 2150 RC print processor and 3 boxes which contain one jug of
Ilford developer and one jug of Ilford fixer each. This baby is in
fantastic condition and I even cleaned it so it is all sparkly and
ready to move into your darkroom. You don't even have to kick the
tires, plus we even have the original owners manual.

A lot of film holders for Zeiss, JEOL and maybe Hitachi. Some of
these holders are for the more vintage TEM models.

If you have any questions, please e-mail Andy Kloiber at andykloiber-at-yahoo.com.

I will be on vacation next week so Andy can help you.

First come, first served and all that jazz.

More items to come as we slowly clean the darkroom out.

Enjoy your weekend,

Paula
--
Paula Sicurello, HTL (ASCP)
Supervisor, Electron Microscope Laboratory
Duke University Health System
Rm.#M251 Duke South, Green Zone
Durham, North Carolina 27710
P: †919.684.2091


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From: r.sims-at-auckland.ac.nz
Date: Thu, 6 Jan 2011 18:32:41 -0600
Subject: [Microscopy] viaWWW: JEOL 840 anode cap position

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Email: r.sims-at-auckland.ac.nz
Name: Ritchie Sims

Organization: The University of Auckland

Title-Subject: [Filtered] JEOL 840 anode cap position

Message: Happy New Year

It's been a while since I pulled the anode cap for cleaning, and I
can neither find the part in the manual where it says to use the
upper or lower position for what accelerating voltage nor remember
which height I used.

Can someone remind me which position should be used for 15KV operation?

cheers
Ritchie

ps I can't seem to post in the normal way and our computer guy is on
holiday. Something to do with hidden attachments, I think

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From: oshel1pe-at-cmich.edu
Date: Fri, 7 Jan 2011 07:04:45 -0600
Subject: [Microscopy] Re: viaWWW: JEOL 840 anode cap position

Contents Retrieved from Microscopy Listserver Archives
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Ritchie,

For our 840A with tungsten emitter:
use the upper position for 6kV accelerating voltage and *less*

use the lower position for over 6kV

Arcing occurs/can occur if the upper position is used with kV} 6kV.

Phil

} Email: r.sims-at-auckland.ac.nz
} Name: Ritchie Sims
}
} Organization: The University of Auckland
}
} Title-Subject: [Filtered] JEOL 840 anode cap position
}
} Message: Happy New Year
}
} It's been a while since I pulled the anode cap for cleaning, and I
} can neither find the part in the manual where it says to use the
} upper or lower position for what accelerating voltage nor remember
} which height I used.
}
} Can someone remind me which position should be used for 15KV operation?
}
} cheers
} Ritchie
}
} ps I can't seem to post in the normal way and our computer guy is on
} holiday. Something to do with hidden attachments, I think

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: protrain-at-emcourses.com
Date: Fri, 7 Jan 2011 07:22:36 -0600
Subject: [Microscopy] viaWWW: JEOL 840 anode cap position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ritchie

I do not know the exact position of the anode but the "generic" conversion
for a SEM is 1mm for every 2kV anode to cathode distance!

Hope this helps?

On another note I too have had then hidden attachment rejection on several
occasion recently, could there be a problem?

Steve

Steve Chapman FRMS
Senior Consultant Protrain
For consultancy and training in electron microscopy world wide
Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967
www.emcourses.com



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Email: r.sims-at-auckland.ac.nz
Name: Ritchie Sims

Organization: The University of Auckland

Title-Subject: [Filtered] JEOL 840 anode cap position

Message: Happy New Year

It's been a while since I pulled the anode cap for cleaning, and I
can neither find the part in the manual where it says to use the
upper or lower position for what accelerating voltage nor remember
which height I used.

Can someone remind me which position should be used for 15KV operation?

cheers
Ritchie

ps I can't seem to post in the normal way and our computer guy is on
holiday. Something to do with hidden attachments, I think

Login Host: 130.216.59.18
---------------------------------------------------------------------------

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From: r.sims-at-auckland.ac.nz
Date: Fri, 7 Jan 2011 08:05:37 -0600
Subject: [Microscopy] viaWWW: Out-of-Office auto replies

Contents Retrieved from Microscopy Listserver Archives
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Email: r.sims-at-auckland.ac.nz
Name: Ritchie Sims

Organization: The University of Auckland

Title-Subject: [Filtered] Out-of-Office auto replies

Message: Wow!

I got 21 out-of-office auto-replies to my JEOL 840 anode cap
question, and no reply (yet) with information.

I guess I'll get another 21 auto-replies to this one, too.

May I quote the Listserver FAQs?

"2.) DO NOT set your Email Program to automatically reply to all
messages, or to request a return receipt. If either of these are
done, you run the risk of creating an Email loop within the system.
This may result in a message bouncing through the system for several
days, filling up everyone's mail box! "

And it kinda puts people off posting, too.

cheers
Ritchie



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From: patpxs-at-gmail.com
Date: Fri, 7 Jan 2011 11:41:51 -0600
Subject: [Microscopy] Denton Bell Jar

Contents Retrieved from Microscopy Listserver Archives
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Hello Listers,

I have found a brand new, still in the box with it's bubblewrap and
packing peanuts, 11.5 inch inner diameter, 0.25 inch side wall Denton
bell jar. I am giving it away, you pay the shipping.

Any takers?

Contact Andy Kloiber at andykloiber-at-yahoo.com

Have a greatr weekend!

Paula

Paula Sicurello, HTL (ASCP)
Supervisor, Electron Microscope Laboratory
Duke University Health System
Rm.#M251 Duke South, Green Zone
Durham, North Carolina 27710
P: †919.684.2091


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From: NEERAJG-at-clemson.edu
Date: Fri, 7 Jan 2011 14:16:31 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
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Happy New Year Everyone!

I recently read this article about Ernst Ruska and thought of sharing it with the list, many of you may have already read this but it's truly a fascinating read.

http://www.mpg.de/english/illustrationsDocumentation/multimedia/mpResearch/2006/heft03/Electron_Microscopy_Ernst_Ruska.pdf

Best Regards,

Neeraj.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos ††





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From: ron.doole-at-materials.ox.ac.uk
Date: Mon, 10 Jan 2011 07:00:07 -0600
Subject: [Microscopy] 2 TEM positions available at Oxford - UK

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Two positions are available in electron microscopy at the Department of Materials, University of Oxford, UK:

Departmental Lecturer in Aberration Corrected Electron Microscopy
Grade 8 / Salary £36,715 to £43,840 pa / Post Ref: DJ10/29

Senior Support Scientist in Electron Microscopy
Grade 8 / Salary £36,715 to £43,840 pa / Post Ref: DJ10/30

See http://www.materials.ox.ac.uk/vacancies.html for further details.


Dr Peter Nellist {peter.nellist-at-materials.ox.ac.uk}
University Lecturer in Materials
Tutorial Fellow at Corpus Christi College
Department of Materials
University of Oxford
Parks Road
OXFORD
OX1 3PH
UK

Phone: +44 (0)1865-273656
Fax: +44 (0)1865-283329

Sent on behalf of Pete Nellist.

Mr. Ron Doole Department of Materials
Senior Instrumentation Engineer. University of Oxford.
Phone +44 (0) 1865 273701 Parks Road. Oxford. OX1 3PH
Fax +44 (0) 1865 283333 ron.doole-at-materials.ox.ac.uk

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From: cross-at-tru.ca
Date: Mon, 10 Jan 2011 17:12:53 -0600
Subject: [Microscopy] LM/Teaching

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Hello "Scopenerds"!

Anyone out there use the "Labomed LX400" for lab teaching?

Any testimonials either pro and con in a second year lab setting re: performance and durability?

Please respond privately to me cross-at-tru.ca

Your comments will be much appreciated!

Sincerely,
Cindy







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From: oshel1pe-at-cmich.edu
Date: Tue, 11 Jan 2011 10:56:40 -0600
Subject: [Microscopy] Fwd: Ask-A-Microscopist - SEM access needed, mid-Long Island NY

Contents Retrieved from Microscopy Listserver Archives
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} Date: Mon, 10 Jan 2011 16:08:32 -0800 (PST)
} From: Richard Kurtz {rkurtz-at-commack.k12.ny.us}
} To: oshel1pe-at-cmich.edu
} Subject: Ask-A-Microscopist
}
} Below is the result of your form, submitted on Monday, January 10,
} 2011 at 04:08:30 PM.
}
} realname - Richard Kurtz
} Email - rkurtz-at-commack.k12.ny.us
} ORGANIZATION - Commack High School
} EDUCATION - 9-12th Grade High School
} LOCATION - Commack, NY, USA
} SUBJECT_OF_QUESTION - image of a insect foot pad
} QUESTION - To Whom it may concern,
}
} I am a high school science teacher at Commack High School on Long Island, NY
} I have a pair of students investigating the walking ability of the
} Indian walking stick. The have the ability to walk on a host of
} materials upside down and vertically.
}
} They and I wanted to find a place that would be able to look at
} their footpads microscopically.
} We are very interested in their surface, what structures are on
} their feet. We want to know
} were we may be able to find a place that would be able and willing
} to help us with this.
}
} I have no idea how this could happen but just asking.
}
} Any advice or suggestions that you could provide would be great
}
} Thanks so much
}
--
***************************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
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**any reply should go directly to the poster**
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Using the "reply" function in your email does *not* send your answer
to the person asking the question.
Please copy their email address from their question.
****************************************************************************************

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From: sjrobin-at-illinois.edu
Date: Tue, 11 Jan 2011 11:15:35 -0600
Subject: [Microscopy] Fwd: Ask-A-Microscopist - SEM access needed,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This is something we could do as part of a Bugscope session, and I
invite you to apply.

Thank you

Scott, with Bugscope


--
Microscopy Suite | Imaging Technology Group |
Beckman Institute for Advanced Science and Technology |
University of Illinois at Urbana-Champaign |
405 North Mathews Avenue, Urbana IL 61801 |
217 265 5071 | 217 244 6219 (fax)
sjrobin-at-illinois.edu | http://itg.beckman.illinois.edu |
http://bugscope.beckman.illinois.edu




-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Tuesday, January 11, 2011 11:09 AM
To: Robinson, Scott J.

} Date: Mon, 10 Jan 2011 16:08:32 -0800 (PST)
} From: Richard Kurtz {rkurtz-at-commack.k12.ny.us}
} To: oshel1pe-at-cmich.edu
} Subject: Ask-A-Microscopist
}
} Below is the result of your form, submitted on Monday, January 10,
} 2011 at 04:08:30 PM.
}
} realname - Richard Kurtz
} Email -
} ORGANIZATION - Commack High School
} EDUCATION - 9-12th Grade High School
} LOCATION - Commack, NY, USA
} SUBJECT_OF_QUESTION - image of a insect foot pad QUESTION - To Whom it
} may concern,
}
} I am a high school science teacher at Commack High School on Long
} Island, NY I have a pair of students investigating the walking ability
} of the Indian walking stick. The have the ability to walk on a host of

} materials upside down and vertically.
}
} They and I wanted to find a place that would be able to look at their
} footpads microscopically.
} We are very interested in their surface, what structures are on their
} feet. We want to know were we may be able to find a place that would
} be able and willing to help us with this.
}
} I have no idea how this could happen but just asking.
}
} Any advice or suggestions that you could provide would be great
}
} Thanks so much
}
--
************************************************************************
***************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely not a
member the listserver, and **any reply should go directly to the
poster** as well as to the list.
Using the "reply" function in your email does *not* send your answer to
the person asking the question.
Please copy their email address from their question.
************************************************************************
****************

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From: lgiannuzzi-at-comcast.net
Date: Tue, 11 Jan 2011 19:53:19 -0600
Subject: [Microscopy] viaWWW: FIB/SEM Specialist: open position at Weatherford

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Email: lgiannuzzi-at-comcast.net
Name: Lucille Giannuzzi

Organization: L.A. Giannuzzi & Associates LLC

Title-Subject: [Filtered] FIB/SEM Specialist: open position at Weatherford

Message: FIB-SEM Specialist
Weatherford Laboratories
Houston, TX
Description: Weatherford Laboratories is seeking
qualified candidates to fill the position of
FIB-SEM Specialist at its Houston, Texas
facility. The FIB-SEM Specialist is responsible
for operating and maintaining a dual-column
Focused Ion Beam (FIB) microscope / Scanning
Electron Microscope (SEM) analytical instrument
primarily to provide characterization of rock
material from unconventional reservoirs. The FIB
microscopy work includes in-situ high resolution
imaging, micro-chemical analysis, and
site-specific 3-D feature analysis, utilizing
energy dispersive spectrometry (EDS) / secondary
and backscatter electron characterization
techniques. The FIB Microscopist will utilize
their skills with dual-column FIB /electron
microscopy to evaluate rock properties; perform
data /image processing, including 3-D
reconstruction and visualization of FIB
imaging/analytical data; analyze and interpret
comprehensive and multiple datasets; and
communicate results to clients by oral and
written reports.
Qualifications: A bachelorŪs degree in a relevant
scientific area is required, advanced degree
preferred. Experience with operation of a
dual-column FIB-SEM microscope is required. Image
processing and geologic background a strong plus.
Salary/Benefits: Commensurate with degree and
experience. Company provides competitive benefit
packages.
Salary Range: Commensurate with degree and experience.
Contact Information:
Name: LouAnn Reid
Company: Weatherford Laboratories
Address: 5200 N. Sam Houston Pkwy W., Suite 500, Houston, TX 77086
Email: louann.reid-at-weatherfordlabs.com
Website: www.weatherford.com to apply for position
Open Search: 12/9/10
Close Search: Until filled


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From: dsherman-at-purdue.edu
Date: Tue, 11 Jan 2011 20:12:30 -0600
Subject: [Microscopy] Commercial microscopy rates

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

We are in the process of revising our recharge rates for our core microscopy
facility. Although we do not solicit imaging projects from external
customers, we do get some...usually from former students or companies
associated with university faculty.

Our major instrumentation was purchased with the help of federal grants. The
granting agencies do not have a problem with our using them for external
projects provided that all internal clients funded by that agency,
especially those whose projects were used for justification, have as much
access as desired. However, they stipulate that any charges be equivalent
to those of commercial providers.

Thus I need to talk to for-profit companies, who provide SEM and TEM,
regarding current rates so that we can comply with this mandate. I would
greatly appreciate your contacting me if you will be willing to help me in
this task.

Thanks,
Debby

---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy





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From: dsherman-at-purdue.edu
Date: Tue, 11 Jan 2011 20:15:54 -0600
Subject: [Microscopy] Staining somatic cells in milk

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
We have someone who wants images of somatic cells (mainly leukocytes) in
milk using light microscopy. I am looking for a staining method and would
appreciate any hints to accomplish this. This is a bit out of my area with
my being primarily an electron microscopist.

Debby
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy/




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6, 28 -- Subject: Staining somatic cells in milk
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From: nizets2-at-yahoo.com
Date: Wed, 12 Jan 2011 05:50:12 -0600
Subject: [Microscopy] Staining somatic cells in milk

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debby!

The main question is: can you isolate the cells, stain them and put them back in
the milk?

If not, here are 2 possibilities:

The easiest one†I can see is to incubate the sample with a DNA dye such as DAPI
or Hoechst. These dyes are not expensive and are used by all microscopists doing
fluorescence.
Just mix the milk with a little bit of dye and observe...that's all!
The only drawback is that you need a fluorescence microscope, but nowadays they
are everywhere! I hope that the milk doesn't have an intrinsic autofluorescence
in the UV range!

Alternatively†tetrazolium salts can be metabolized by live cells into formazan,
which are dyes.†Some formazan dyes are soluble, others are not. Just choose an
insoluble, like MTT. In this case you will only stain live cells, not the dead
one.
Tetrazolium salts are not expensive.

You won't be able to make the difference with germinal cells, though :-D
I would avoid to use†dyes†which stain the membranes, since they may concentrate
in the lipid droplets of the milk.

Regards,
Stephane



----- Original Message ----
X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu}
To: nizets2-at-yahoo.com
Sent: Wed, January 12, 2011 3:18:44 AM


Hi all,
We have someone who wants images of somatic cells (mainly leukocytes) in
milk using light microscopy.† I am looking for a staining method and would
appreciate any hints to accomplish this. This is a bit out of my area with
my being primarily an electron microscopist.

Debby
--
Debby Sherman, Director† † † † † † † Phone: 765-494-6666
Life Science Microscopy Facility† † FAX:† 765-494-5896
Purdue University† † † † † † † † † E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy/




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6, 28 -- To: "message to: MSA list" {microscopy-at-microscopy.com}
6, 28 -- Date: Tue, 11 Jan 2011 21:15:51 -0500
6, 28 -- Subject: Staining somatic cells in milk
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From: mseabul-at-aerotek.com
Date: Wed, 12 Jan 2011 10:03:33 -0600
Subject: [Microscopy] viaWWW: Job Opening: Applications Scientist TEM/STEM of catalysts

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Email: mseabul-at-aerotek.com
Name: Melanie Seabul

Organization: Aerotek Scientific

Title-Subject: [Filtered] Job Opening: Applications Scientist
TEM/STEM of catalysts

Message: Hi! My name is Melanie and I work for Aerotek Scientific, a
technical staffing firm in the Chicagoland area. I currently have an
opening for a microscopy position in the area. Some details on this
position are as follows:

-individual must be familiar with working with an STEM/TEM electron
microscope, in support of the materials and catalyst development of
the company
-Individual will collaborate with and assist senior electron
microscopists in the development of new capabilities for a new probe
aberration corrected STEM/TEM microscope
-Will be designing and conducting experiments and techniques using
this microscope
-operating the aberration corrected microscope for characterization
of nanomaterials and catalysts
-develope advanced applications for STEM, EELS, EDS tomography, and
in-situ for beam sensitive nanomaterials

Best candidate will have Ph.D. in Materials Science, chemistry,
physics or metallurgy with specialization in electron microscopy,
experience with at least one of EDS, EELS, tomography or in-situ
techniques and 0-5 years in a related field. Strong understanding of
instrumentation and other characterization techniques such as X-ray
diffraction are a plus.

Please let me know if you or anyone that you know may be interested
and qualified for this position, I am actively seeking great
candidates! Thank you!

Melanie Seabul
Aerotek Scientific
1790 Nations Drive, Suite 116
Gurnee, IL 60031

Direct Line: 847-782-5447
E-mail: mseabul-at-aerotek.com


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From: mseabul-at-aerotek.com
Date: Wed, 12 Jan 2011 10:03:54 -0600
Subject: [Microscopy] viaWWW: Job Opening: Applications Scientist Metallurgy/Corrosion

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Email: mseabul-at-aerotek.com
Name: Melanie Seabul

Organization: Aerotek Scientific

Title-Subject: [Filtered] Job Opening: Applications Scientist
Metallurgy/Corrosion

Message:
Hi! My name is Melanie and I work for Aerotek Scientific, a technical
staffing firm in the Chicagoland area. I currently have a job
opportunity for a Metallurgy/Corrosion scientist. Some details on
this position are as follows:

-individual must have 10 years of on the job experience as a
metallurgist and corrosion scientist
-strong analytical chemistry skills
-must have strong understanding of the degradation of metal at its
surface through interaction with chemicals
-working on project basis, researching and studying structures and
chem. Properties of metals
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analysis, failure investigation, materials conservation, organic
chemistry, analytical chemistry, petrographic evaluation
-will be involved in running these tests: nondestructive evaluation,
water penetration testing, strain and fracture monitoring, vibration
monitoring, load testing, blast monitoring, frequency identification

The best candidate will have a masters degree, or higher, in a
related scientific field with 10 years of experience. Please let me
know if you or anyone that you know may be interested and qualified
for this position as I am actively seeking great candidates! Thank
you!

Melanie Seabul
Aerotek Scientific
1790 Nations Drive, Suite 116
Gurnee, IL 60031

Direct Line: 847-782-5447
E-mail: mseabul-at-aerotek.com


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From: NEERAJG-at-clemson.edu
Date: Wed, 12 Jan 2011 13:01:16 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
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Hi Carol,

As the article describes, the early version of the instrument could only reach modest resolutions and we have to remember that the thermal advancement Ultramicrotome by Porter and Bloom was reported in 1953. We know now what the combination of TEM and ultramicrotome did for biology and other fields, and gradually many crucial discoveries were made using the TEM which eventually could attain atomic resolutions. This is somewhat similar to discovery of LASER, many of the key people who worked on the LASER during its conception were awarded the Nobel prize later (Luckily the TEM didn't go through the lawsuits and patent battles that the LASER did). This may be true of our times too, we have yet to fully grasp the potential of recent advances in super-resolution optical imaging.

Best,

Neeraj.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos





-----Original Message-----
X-from: Carol Heckman [mailto:heckman-at-bgsu.edu]
Sent: Sunday, January 09, 2011 12:00 PM
To: Neeraj Gohad

Happy New Year Everyone!

I recently read this article about Ernst Ruska and thought of sharing it with the list, many of you may have already read this but it's truly a fascinating read.

http://www.mpg.de/english/illustrationsDocumentation/multimedia/mpResearch/2006/heft03/Electron_Microscopy_Ernst_Ruska.pdf

Best Regards,

Neeraj.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos





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From: schooley-at-mcn.org
Date: Wed, 12 Jan 2011 13:22:17 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sorry, Neera - the Porter-Blum microtome was mechanical advance.
Simple, reliable, unbreakable.

Caroline

} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

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From: wmassover-at-anl.gov
Date: Wed, 12 Jan 2011 13:24:24 -0600
Subject: [Microscopy] A New Resource for Trying to Improve Resolution with Biospecimens

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

January 12, 2011

To All Microscopists Researching High Resolution Biological Structure:

I call your attention to a new Special Issue of the Elsevier journal, MICRON, which has just been published (Volume 42, number 2: February, 2011). This originated from a realization that several different types of modern microscopes now can readily achieve atomic and sub-√Öngstrom levels of resolution with many inorganic specimens; since the resolution with biological specimens still only rarely obtains better than 3√Ö (0.3nm), this deficient resolution must not be caused by the instrument, but rather is due to inadequacies in specimen preparation and in structural preservation during data collection. These two important subjects are what the Special Issue deals with, in the context of a wide variety of biospecimens and use of several different kinds of microscopes (TEM, STEM, SEM, and AFM).

The 11 new review articles within this Special Issue concern the many practical problems for trying to produce better specimen preparation and preservation. Each is written by expert practicing microscopists. Many examples of research results and technical advances are illustrated by selected micrographs, diagrams, and charts, and are documented by extensive literature citations. Practical interest is expected from research students, technicians, and postdocs, as well as from experienced microscopists. We hope that these new reviews will be both informative and stimulating for all biological microscopists trying to obtain structural data with even higher resolution levels than are currently possible.

A complete table of contents follows this message. This Special Issue is included with all regular subscriptions to MICRON, and now is available to those at many institutions via the Science Direct website
(http://www.sciencedirect.com).

Sincerely, and Happy New Year (2011),

BILL MASSOVER

William H. Massover
Guest Editor, MICRON
wmassover-at-anl.gov








Special Issue

MICRON Volume 42(2) February 2011: Contents

"Biological Specimen Preparation and Preservation for High Resolution Microscopies"


97 Introduction to Special Issue of Micron: “Biological specimen preparation and preservation for
high resolution microscopies‚ÄĚ

W.H. Massover

100 Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope
with a liquid-helium cooled stage

K. Mitsuoka

107 Electron cryomicroscopy of membrane proteins: Specimen preparation for two-dimensional
crystals and single particles

I. Schmidt-Krey and J.L. Rubinstein

117 Negative staining and cryo-negative staining of macromolecules and viruses for TEM

S. De Carlo and J. Robin Harris

132 Specimen preparation for electron diffraction of thin crystals

H. Wang and K.H. Downing

141 New and unconventional approaches for advancing resolution in biological transmission
electron microscopy by improving macromolecular specimen preparation and preservation

W.H. Massover

152 Cryo-electron tomography on vitrified sections: A critical analysis of benefits and limitations for
structural cell biology

C. Bouchet-Marquis and A. Hoenger

163 Preparation and high-resolution microscopy of gold cluster labeled nucleic acid conjugates and
nanodevices

R.D. Powell and J.F. Hainfeld

175 Low voltage high-resolution SEM (LVHRSEM) for biological structural and molecular analysis

H. Schatten

186 Assessing the structure and function of single biomolecules with scanning transmission electron
and atomic force microscopes

S.A. M√ľller, D.J. M√ľller and A. Engel

196 Preparation of DNA and nucleoprotein samples for AFM imaging

Y.L. Lyubchenko


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From: r.gilbert-at-auckland.ac.nz
Date: Wed, 12 Jan 2011 13:43:14 -0600
Subject: [Microscopy] Staining somatic cells in milk

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I did a few years looking at milk in-situ in mammary gland including lactating and involuting where we saw lots of somatic cells.

Hoechst or DAPI will work fine, there is not a significant problem with autofluorescence even in the UV range, and you can differentiate the types of cells a little by the staining pattern.

If you do not want to go the fluorescence root you could use plain old heamotoxylin and eosin. This has an added advantage, leucocytes are highly eosinophillic and are characterised very well with standard H&E. You will also get good ID on other cell types.

If you do not want spin down your cells you could try a standard blood smear prep on a coated slide and fix them on with formalin or paraformaldehyde. Alternatively you could add low melting point agar or agarose and set them as a gel. This will allow processing through paraffin if you need (although you lose your water and milk lipids of course.

Thanks

Ray


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Thursday, 13 January 2011 1:00 a.m.
To: Ray Gilbert

Hi Debby!

The main question is: can you isolate the cells, stain them and put them back in
the milk?

If not, here are 2 possibilities:

The easiest one†I can see is to incubate the sample with a DNA dye such as DAPI
or Hoechst. These dyes are not expensive and are used by all microscopists doing
fluorescence.
Just mix the milk with a little bit of dye and observe...that's all!
The only drawback is that you need a fluorescence microscope, but nowadays they
are everywhere! I hope that the milk doesn't have an intrinsic autofluorescence
in the UV range!

Alternatively†tetrazolium salts can be metabolized by live cells into formazan,
which are dyes.†Some formazan dyes are soluble, others are not. Just choose an
insoluble, like MTT. In this case you will only stain live cells, not the dead
one.
Tetrazolium salts are not expensive.

You won't be able to make the difference with germinal cells, though :-D
I would avoid to use†dyes†which stain the membranes, since they may concentrate
in the lipid droplets of the milk.

Regards,
Stephane



----- Original Message ----
X-from: "dsherman-at-purdue.edu" {dsherman-at-purdue.edu}
To: nizets2-at-yahoo.com
Sent: Wed, January 12, 2011 3:18:44 AM


Hi all,
We have someone who wants images of somatic cells (mainly leukocytes) in
milk using light microscopy.† I am looking for a staining method and would
appreciate any hints to accomplish this. This is a bit out of my area with
my being primarily an electron microscopist.

Debby
--
Debby Sherman, Director† † † † † † † Phone: 765-494-6666
Life Science Microscopy Facility† † FAX:† 765-494-5896
Purdue University† † † † † † † † † E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy/




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From: NEERAJG-at-clemson.edu
Date: Wed, 12 Jan 2011 13:52:50 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My mistake, I meant mechanical, Porter-Blum MTI was indeed mechanical, but you get the point. What is the name of Ruska's book that you mentioned.

Best,

Neeraj.

-----Original Message-----
X-from: schooley-at-mcn.org [mailto:schooley-at-mcn.org]
Sent: Wednesday, January 12, 2011 2:27 PM
To: Neeraj Gohad

Sorry, Neera - the Porter-Blum microtome was mechanical advance.
Simple, reliable, unbreakable.

Caroline

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From: RossLM-at-missouri.edu
Date: Wed, 12 Jan 2011 14:41:43 -0600
Subject: [Microscopy] 4-Day SEM Training Course - Univ of Missouri 3/29/11

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Electron Microscopy Core (EMC) at the University of Missouri Columbia, MO is proud to host an intensive short course:

Understanding and Optimizing FESEM and EDS Performance (Basic to Advanced)

Course Dates: Tuesday March 29 Ė Friday April 1, 2011
Cost: $1200 includes all course material (handouts and CD) and hands-on instrument instruction along with a reception and banquet
Enrollment is limited to 12 participants!

Course Instructor: Steve Chapman - Protrain
Steve Chapman formed Protrain in 1982 and is the senior consultant in electron microscopy. Steve first became involved with electron microscopes in 1964 at the University of Birmingham, England, then as an EM service engineer in the UK, and afterwards moved into sales and marketing where he worked with a number of electron microscope and accessory manufacturers in the demonstration and application fields. Running courses in many parts of the world, north and south of the equator, he has written six books including those on the operation and maintenance of scanning and transmission electron microscopes. His publications include papers on a range of electron microscopy subjects from instrument design through a wide range of applications and more recently on Quality in Electron Microscopy.

In a round-table illustrated discussion, you will be taken through the theory and practice of the operation of the scanning electron microscope, from basic through to advanced levels. In addition, the course will cover specimen preparation techniques and coating procedures. The EMC houses two field emission SEMís, a Hitachi cold-field SEM (S-4700) and a FEI thermal FE SEM (Quanta 600 ESEM) with a Thermo System Six EDS. Practical periods will offer you the opportunity to evaluate instrument performance over a wide range of accelerating voltages and make appropriate modifications to the set-up in order to optimize the instrumentís performance. In this way, gun and specimen geometry may be better understood and you will be able to raise your skill levels to bring better performance from an instrument than the manufacturer claims. Other topics to be covered are low vacuum operation and imaging along with qualitative EDS analysis.

All students will operate the instruments themselves and develop their own talents. Under the eagle eye and guidance of Steve, participants should come away from this course with the understanding and abilities necessary to obtain the highest quality imaging and chemical analysis from their SEM/EDS systems.

Additional Support by: FEI, Hitachi High Technologies America, and Thermo Scientific.

For more Information or to register go to: http://www.emc.missouri.edu/ or contact me at rosslm-at-missouri.edu or at (573) 882-4777

Lou Ross
--
Sr. Research Specialist
University of Missouri
Electron Microscopy Core Facility
W136 Veterinary Medicine Building
Columbia, MO 65211
573.882.4777, fax 573.884.2227
RossLM-at-missouri.edu
http://www.emc.missouri.edu/



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From: paul_hazelton-at-umanitoba.ca
Date: Wed, 12 Jan 2011 17:34:36 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Neeraj et al

To follow up on what Caroline said, thermal advance was first brought
out by LKB, and I believe was developed by Sjostrand.

Regards the Nobel Prize. Story I heard but could never get confirmed
was that the failure to give Ruska the prize before the 1980's started
as political, proceeded to oversight, and in the end became an
embarrassment.

Ernst Ruska remained in Germany during WWII, and worked with the German
war effort. Please remember that one of the major uses of the
microscope in Germany and the US was in the development of the atomic
bomb. Because of this, in the time leading up to, during and
immediately after the war giving the prize to someone from Germany was
not very acceptable. This was the story to me in 1969, when I first
started doing EM. Ruska was, of course still alive, but as told, would
not be a candidate for the prize.

Over the ensuing years Ruska sort of got forgotten. Then came the
development of Scanning Tunneling EM. In 1986, when the Academy was
considering the award of the Prize in Physics to Binnig and Rohrer they
realized, much to many people's chagrin, that Ruska had never been
recognized. Whatever his personal feelings may have been, his speech
was gracious, and only said:

"Here, I only want to emphasize my impression that the scanning tunnel
electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously
been accepted much faster by scientific colleagues than electron
microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech,
December 10, 1986)

Sadly, the failure to act in a more timely fashion meant that others
such as Hans Busch and Max Knoll (the only names that jump to mind just
now) did not also receive the recognition they deserved.

Politics in the award of the Prize in all fields has been a long
tradition. It still carries on. Witness the award of the Prize in
Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to
Henry Kissinger in 1973, without recognition of Nixon, who, for all the
wrong things he did, directed the negotiations which lead to the end of
the Viet Nam war and ultimately deserved to share in this recognition.
And that is a heck of a statement for someone considered to be
politically to the left of centre.

Paul Hazelton

Paul R. Hazelton, PhD
Gastroenteric Diseases Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w)
204-489-6924 (h)
Cell: 204-781-6982


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From: paul_hazelton-at-umanitoba.ca
Date: Wed, 12 Jan 2011 17:55:09 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The title of the book is The early development of electron lenses and
electron microscopy. The English translation was by Mulvey, and it was
published in 1980.

My copy was borrowed 25 years ago, and I have not seen it since. It is
long out of print.

paul hazelton

Paul R. Hazelton, PhD
Gastroenteric Diseases Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w)
204-489-6924 (h)
Cell: 204-781-6982

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From: brad-at-nanounity.com
Date: February 3, 2011
Subject: [Microscopy] viaWWW: NCSM kick-off meeting

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
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please copy both brad-at-nanounity.com as well as the MIcroscopy Listserver
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Email: brad-at-nanounity.com
Name: Brad Rangell

Organization: NCSM

Title-Subject: [Filtered] NCSM kick-off meeting

Message: We are happy to announce the long
anticipated reinstatement of the Northern
California Chapter of the Society for Microscopy.
Our first meeting will be an informal reception
for you to get re-acquainted with your microscopy
colleagues. During this get-together we want to
begin to formalize membership and to obtain ideas
for upcoming events for 2011. We anticipate the
meeting to be a mix of networking and
socializing, exchange of information and input
from the membership on objectives and direction
for this chapter. With this in mind here is the
initial outline of the meeting. ...


Time: 6:00 to 8:00 PM

Location: International Building, SRI
International, 333 Ravenswood Ave., Menlo Park,
CA 94025; directions to follow.

Membership: $40 regular member, $20 student member, $100 corporate membership

Social hours: 6:00 to 7:00 PM hors de' oeuvres and beverages provided

Business items: 7:00 Ů 8:00 Informal Discussion of the following topics:

Ô Officer Elections Ů we would like to ask
members to provide suggestions of individuals to
become elected officers. These can be submitted
during the course of the evening.
Ô Discussion of By Laws for the society
Ô Discussion of future meetings and events in
2011 including subject matter and locations

Please send RSVP and questions to: Steve
Samuelsson steven.samuelsson-at-sri.com or Brad
Rangell brad-at-nanounity.com.

Please forward this notice to friends and
colleagues who may have interest in joining NCSM
and attending this kick-off meeting.


Login Host: 67.170.208.232
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From: NEERAJG-at-clemson.edu
Date: Wed, 12 Jan 2011 20:29:55 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
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Paul,

Thanks for sharing the story as well as the title of the book. The story is really interesting and sheds some light on why the prize was awarded so late. As we are all aware, any human endeavor is fraught with politics and I am sure the Nobel committees aren't immune to it. On the subject of Nobel piece prizes, a pretty major one they missed (according to the Nobel foundation itself) was Mahatma Gandhi (if any ones interested http://nobelprize.org/nobel_prizes/peace/articles/gandhi/ ).

Best Regards,

Neeraj.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos





-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Wednesday, January 12, 2011 6:40 PM
To: Neeraj Gohad

Neeraj et al

To follow up on what Caroline said, thermal advance was first brought
out by LKB, and I believe was developed by Sjostrand.

Regards the Nobel Prize. Story I heard but could never get confirmed
was that the failure to give Ruska the prize before the 1980's started
as political, proceeded to oversight, and in the end became an
embarrassment.

Ernst Ruska remained in Germany during WWII, and worked with the German
war effort. Please remember that one of the major uses of the
microscope in Germany and the US was in the development of the atomic
bomb. Because of this, in the time leading up to, during and
immediately after the war giving the prize to someone from Germany was
not very acceptable. This was the story to me in 1969, when I first
started doing EM. Ruska was, of course still alive, but as told, would
not be a candidate for the prize.

Over the ensuing years Ruska sort of got forgotten. Then came the
development of Scanning Tunneling EM. In 1986, when the Academy was
considering the award of the Prize in Physics to Binnig and Rohrer they
realized, much to many people's chagrin, that Ruska had never been
recognized. Whatever his personal feelings may have been, his speech
was gracious, and only said:

"Here, I only want to emphasize my impression that the scanning tunnel
electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously
been accepted much faster by scientific colleagues than electron
microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech,
December 10, 1986)

Sadly, the failure to act in a more timely fashion meant that others
such as Hans Busch and Max Knoll (the only names that jump to mind just
now) did not also receive the recognition they deserved.

Politics in the award of the Prize in all fields has been a long
tradition. It still carries on. Witness the award of the Prize in
Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to
Henry Kissinger in 1973, without recognition of Nixon, who, for all the
wrong things he did, directed the negotiations which lead to the end of
the Viet Nam war and ultimately deserved to share in this recognition.
And that is a heck of a statement for someone considered to be
politically to the left of centre.

Paul Hazelton

Paul R. Hazelton, PhD
Gastroenteric Diseases Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w)
204-489-6924 (h)
Cell: 204-781-6982


==============================Original Headers==============================
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27, 37 -- From NEERAJG-at-clemson.edu Wed Jan 12 20:29:55 2011
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From: cross-at-tru.ca
Date: Wed, 12 Jan 2011 22:20:08 -0600
Subject: [Microscopy] Re: Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks for all of this - a very interesting thread, indeed!

Cheers,
Cindy

} } } {NEERAJG-at-clemson.edu} 12/01/2011 6:39 pm } } }



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Paul,

Thanks for sharing the story as well as the title of the book. The story is really interesting and sheds some light on why the prize was awarded so late. As we are all aware, any human endeavor is fraught with politics and I am sure the Nobel committees aren't immune to it. On the subject of Nobel piece prizes, a pretty major one they missed (according to the Nobel foundation itself) was Mahatma Gandhi (if any ones interested http://nobelprize.org/nobel_prizes/peace/articles/gandhi/ ).

Best Regards,

Neeraj.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos





-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Wednesday, January 12, 2011 6:40 PM
To: Neeraj Gohad

Neeraj et al

To follow up on what Caroline said, thermal advance was first brought
out by LKB, and I believe was developed by Sjostrand.

Regards the Nobel Prize. Story I heard but could never get confirmed
was that the failure to give Ruska the prize before the 1980's started
as political, proceeded to oversight, and in the end became an
embarrassment.

Ernst Ruska remained in Germany during WWII, and worked with the German
war effort. Please remember that one of the major uses of the
microscope in Germany and the US was in the development of the atomic
bomb. Because of this, in the time leading up to, during and
immediately after the war giving the prize to someone from Germany was
not very acceptable. This was the story to me in 1969, when I first
started doing EM. Ruska was, of course still alive, but as told, would
not be a candidate for the prize.

Over the ensuing years Ruska sort of got forgotten. Then came the
development of Scanning Tunneling EM. In 1986, when the Academy was
considering the award of the Prize in Physics to Binnig and Rohrer they
realized, much to many people's chagrin, that Ruska had never been
recognized. Whatever his personal feelings may have been, his speech
was gracious, and only said:

"Here, I only want to emphasize my impression that the scanning tunnel
electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously
been accepted much faster by scientific colleagues than electron
microscopy fifty years ago." (Ernst Ruska, Nobel Banquet Speech,
December 10, 1986)

Sadly, the failure to act in a more timely fashion meant that others
such as Hans Busch and Max Knoll (the only names that jump to mind just
now) did not also receive the recognition they deserved.

Politics in the award of the Prize in all fields has been a long
tradition. It still carries on. Witness the award of the Prize in
Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to
Henry Kissinger in 1973, without recognition of Nixon, who, for all the
wrong things he did, directed the negotiations which lead to the end of
the Viet Nam war and ultimately deserved to share in this recognition.
And that is a heck of a statement for someone considered to be
politically to the left of centre.

Paul Hazelton

Paul R. Hazelton, PhD
Gastroenteric Diseases Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w)
204-489-6924 (h)
Cell: 204-781-6982


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From: wflai-at-lcsd.gov.hk
Date: Thu, 13 Jan 2011 03:15:40 -0600
Subject: [Microscopy] LM need help to identify termite frass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear list members,

I've collected some light brown low density materials (of size about 1cm3)
from cultural heritage object that mixed with clay. I've done FTIR and
identified the material to be cellulose/wood. It was dispersed in water
and then mount on microscopic glass slide. The particles are irregular in
shape and measured 45-75 microns. I highly suspected they were termite
frass. Is there any reference guide for identification of termite frass?
They are coming from subtropical region in South East asia, that is Hong
Kong. Thank you.

Regards,
Wing Fai

This email message (together with any attachments) is for the designated recipient only. It may contain information that is privileged for the designated recipient. If you are not the intended recipient, you are hereby notified that any use, retention, disclosure, copying, printing, forwarding or dissemination of the message is strictly prohibited. If you have received the message in error, please erase all copies of the message (including attachments) from your system and notify the sender immediately.

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From: oshel1pe-at-cmich.edu
Date: Thu, 13 Jan 2011 13:46:27 -0600
Subject: [Microscopy] Ask-A-Microscopist - donor 'scopes for schools?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} Date: Thu, 13 Jan 2011 11:32:22 -0800 (PST)
} From: Ronald Schwartz {ronmschwartz-at-yahoo.com}
} Subject: Ask-A-Microscopist
}
} Below is the result of your form, submitted on Thursday, January 13,
} 2011 at 11:32:20 AM.
}
} realname - Ronald Schwartz
} Email - ronmschwartz-at-yahoo.com
} ORGANIZATION - Providence, RI museum, US Fish&Wildlife Nature Center
} EDUCATION - 6-8th Grade Middle School
} LOCATION - Wakefield, RI
} SUBJECT_OF_QUESTION - increasing microscope activities
} QUESTION - I am a volunteer at the museum and the nature center. I
} have been trying to initiate and expand microscope activities. I
} volunteer my own equipment and my time. I would like to expand this
} activity both where I volunteer and in the schools, but the
} equipment is limited. Are there places that donate microscopes or
} other equipment like cameras that link between the microscope and a
} computer? The schools, esp. the middle schools, have little or no
} equipment for either science class and/or extra-curricular activity.
} When there are old scopes, teachers also don't seem to be trained in
} using them.

--
***************************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
**any reply should go directly to the poster**
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****************************************************************************************

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From: gradice-at-richmond.edu
Date: Thu, 13 Jan 2011 14:07:12 -0600
Subject: [Microscopy] Source of calcite crystals for demonstrating birefringence?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Anyone know of a source for these? I'm teaching an microscopy course
and I'd like to show this classic demonstration. I got some cheap
crystals from Carolina Biological but they aren't very transparent.


Gary Radice
Department of Biology
University of Richmond
Richmond VA 23173
804-289-8107


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From: sckuehn-at-concord.edu
Date: Thu, 13 Jan 2011 14:33:58 -0600
Subject: [Microscopy] Re: Source of calcite crystals for demonstrating birefringence?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Gary,

A geology department will usually have transparent calcite on hand, but
your institution does not appear to have that program. Transparent
calcite is often referred to as "Iceland Spar." A quick search should
turn up several potential suppliers. Wards, for example, has specimens
in four different size/quality categories with prices that vary accordingly.

Regards,

- Steve Kuehn


gradice-at-richmond.edu wrote:
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Anyone know of a source for these? I'm teaching an microscopy course
} and I'd like to show this classic demonstration. I got some cheap
} crystals from Carolina Biological but they aren't very transparent.
}
}
} Gary Radice
} Department of Biology
} University of Richmond
} Richmond VA 23173
} 804-289-8107


--

----------------
Dr. Stephen C. Kuehn
Research Scientist and Lecturer
Manager, Electron Microprobe Facility
Division of Natural Sciences
Science, room 106

Concord University, Campus Box F20
1000 Vermillion St
PO Box 1000
Athens, WV 24712-1000

http://academics.concord.edu/sckuehn/
sckuehn-at-concord.edu kuehnsc-at-gmail.com
304-384-6322

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From: sckuehn-at-concord.edu
Date: Thu, 13 Jan 2011 15:10:06 -0600
Subject: [Microscopy] Fwd: Source of calcite crystals for demonstrating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Gary and all,

Bart Cannon {cannonmp-at-comcast.net} just asked me to pass along that he
has available plenty of inexpensive, colorless "iceland spar" suitable
for demonstration purposes.

Regards,

- Steve

}
} Gary,
}
} A geology department will usually have transparent calcite on hand, but
} your institution does not appear to have that program. Transparent
} calcite is often referred to as "Iceland Spar." A quick search should
} turn up several potential suppliers. Wards, for example, has specimens
} in four different size/quality categories with prices that vary
} accordingly.
}
} Regards,
}
} - Steve Kuehn


--

----------------
Dr. Stephen C. Kuehn
Research Scientist and Lecturer
Manager, Electron Microprobe Facility
Division of Natural Sciences
Science, room 106

Concord University, Campus Box F20
1000 Vermillion St
PO Box 1000
Athens, WV 24712-1000

http://academics.concord.edu/sckuehn/
sckuehn-at-concord.edu kuehnsc-at-gmail.com
304-384-6322

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From: schooley-at-mcn.org
Date: Thu, 13 Jan 2011 17:49:32 -0600
Subject: [Microscopy] Re: Ask-A-Microscopist - donor 'scopes for schools?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


So I did a little digging on who reported thermal advance in ultramicrotomes first (not which commercial instruments came first) the original paper by Keith Porter and J. Blum published in 1953, A study in Microtomy for electron Microscopy, The Anatomical Record Volume 117, Issue 4, pages 685-709, December 1953, in it Porter and Blum describe two new mechanisms, one is a thermal expansion and the other is mechanical advancement (I have a PDF of this paper if anyone is interested). Here is an excerpt from the Summary of this paper

'Two new microtomes capable of cutting serial sections as thin as 25-50 mu are described. All moving parts in these instruments are supported in unlubricated pivots and the specimen is taken past the cutting edge only on the cutting stroke. These two features more than any others seem responsible for the successful performance of these microtomes. In one instrument, the prototype, the block is advanced to the knife by thermal expansion. In the other, the advancement is controlled mechanically.'

Interestingly enough, Fritiof Sjostrand's paper, A new microtome for ultrathin sectioning for high resolution microscopy, 1953 Experientia 9:114-121 (note the paper also published in 1953) says 'The microtome reported on in this paper represents a further development of the microtome designed by Porter' (http://www.springerlink.com/content/y3267k5456j11117/).

Also found an interesting book, Picture Control: The Electron Microscope and the Transformation of Biology in America by Nicolas Rasmussen, has some interesting history on this matter (see preview, http://books.google.com/books?id=rwC5QiqLS44C&pg=PA127&lpg=PA127&dq=Sjostrand+a+new+ultramicrotome&source=bl&ots=HCTXlObRJ-&sig=G46YfqxY8lrFtBptZDgmxIDbxuA&hl=en&ei=cHAvTfLRBsKSgQfMo-xa&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCwQ6AEwAg#v=onepage&q&f=false


Pretty Interesting!

Best,

Neeraj.


Neeraj V. Gohad, Ph.D.
Postdoctoral Fellow
Okeanos Research Group
Department of Biological Sciences
132 Long Hall
Clemson University
Clemson,SC-29634
Phone: 864-656-3597
Fax: 864-656-0435

Website: http://www.clemson.edu/okeanos











X-from: Paul Hazelton [mailto:hazeltn-at-cc.umanitoba.ca]
Sent: Wednesday, January 12, 2011 4:09 PM
To: Neeraj Gohad

} ----------------------------------------------------------------------------
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Ron -

Bravo for your efforts! Are you aware that 1) MSA has regional
societies, & 2) the New England Society has kits for middle school
microscopy that they circulate to people like you? Their program
chair is Mary McCann
{mccanns-at-tiac.net} , (617)484-7865. If you have difficulty
reaching her, let me know.

I hope that you'll visit the Project MICRO web page (URL below).
There's a lot of advice about suitable equipment. Donated scopes can
be more of a liability than an asset; they are too complex for middle
school teachers & they usually need parts & service.

We're here to help; please ask questions. I'm a retiree, which means
that I have the time to respond to your Email!

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
45301 Caspar Point Road, Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: oshel1pe-at-cmich.edu
Date: Fri, 14 Jan 2011 07:58:34 -0600
Subject: [Microscopy] cathodoluminescence detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

We're in the process of writing a proposal for a new SEM, and will
likely include a cathodoluminescence detector.
As I don't know a whole lot about these, I'd like some input on
performance, brands, models, prices, company service (especially
service) and the like.
Use will be for materials and especially mineralogy research and
teaching in our microscopy program. So the unit needs to be suitable
for research, but robust and user-friendly enough for teaching.

This is not for a NSF or such grant, so cost is also important (low
cost, whatever that is for a good cathodoluminescence detector). But
I don't have a good handle on prices yet - other than that there are
some hideously expensive units that we can't consider.
Vendor replies welcome.

BUT!
**Off-line** please, not to the list.

Although if someone wants to start a discuss on the technique and
uses of cathodoluminescence, I'd be interested in reading it ...

Thanks.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: oshel1pe-at-cmich.edu
Date: Fri, 14 Jan 2011 08:08:03 -0600
Subject: [Microscopy] SDD detectors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

First!
Please reply directly to me, **not** the list.

We are hoping to get a new silicon drift detector (SDD) EDS system
for our SEM. Either as a retro-fit or for a new SEM.
I've been looking at the models available, but I'd like to get some
user input - reliability, user-friendliness, company service, is the
software open-source or proprietary, and how much does the company
charge for software upgrades?
EDS software integration with SEM software/hardware, energy
resolution, light element abilities - all the usual things, but as
actual user experiences.

Is the system tied to a particular computer hardware design? (Our
current system uses an old proprietary circuit board that uses an
obsolete slot/bus, so it can't be upgraded or moved to a new
computer, the whole thing has to be replaced.)

This will be for both research - materials, mineralogy, biology, and
whatever else comes up - and teaching.

Vendor replies welcome. To me, please, not the list.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: cross-at-tru.ca
Date: Fri, 14 Jan 2011 09:33:43 -0600
Subject: [Microscopy] Re: LM/Teaching

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Hi, folks - I just got ONE reply to this post! (One very useful one, mind you). But such a small number of responses is not typical for this group!

Am I to interpret the reduced feedback to meen that we should be wary of Labomed LX400??

Have a great weekend,
Cindy


} } } {cross-at-tru.ca} 10/01/2011 3:22 pm } } }


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Hello Microscopists!

Anyone out there use the "Labomed LX400" for lab teaching?

Any testimonials either pro and con in a second year lab setting re: performance and durability?

Please respond privately to me cross-at-tru.ca

Your comments will be much appreciated!

Sincerely,
Cindy







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From: oshel1pe-at-cmich.edu
Date: Fri, 14 Jan 2011 12:01:41 -0600
Subject: [Microscopy] Fwd: Ask-A-Microscopist - Photomicroscopy training in Florida?

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} Date: Fri, 14 Jan 2011 07:20:51 -0800 (PST)
} From: Norah Silva {norah-at-norahsilva.com}
} Subject: Ask-A-Microscopist - Photomicroscopy training in Florida?
}
} Below is the result of your form, submitted on Friday, January 14,
} 2011 at 07:20:43 AM.
}
} realname - Norah Silva
} Email - norah-at-norahsilva.com
} EDUCATION - Undergraduate College
} LOCATION - Boca Raton, FL 33432
} SUBJECT_OF_QUESTION - Capturing images
} QUESTION - Hello,
} I am a photographer and am going back to school for my BS and am
} very interested in the field of microscopy as well as recomendations
} of where to learn more about capture systems. Are there devices
} compatible with Hasselblad or Canon digital capture systems?
} What is the typical line of study to get in to this field?
}
} Thank you in advance for your advice,
} Norah Silva
}
--
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From: jmlebeau-at-ncsu.edu
Date: Fri, 14 Jan 2011 17:20:45 -0600
Subject: [Microscopy] Postdoctoral Position Available

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A post-doctoral position is available in the LeBeau group at North Carolina State University. The successful candidate will be responsible for the development of scanning transmission electron microscopy techniques in conjunction with studying interfaces, nanoparticles/nanocomposites, and device structures. The candidate will focus on quantitative electron microscopy, determination of heterogeneous interface structures, and exploring chemistry with spectroscopic techniques. Applicants should have demonstrated expertise in problem solving in materials science using a wide range of transmission electron microscopy techniques.

NCSU will soon acquire an aberration corrected microscope for ultra-high resolution STEM imaging and chemical analysis. Other equipment includes a JEOL 2010 STEM/TEM, several conventional TEMs, and a FIB. The position is available starting Jan 1, 2011 and requires a Ph. D. in materials science or a related field. Experience with aberration corrected STEM is desired. Duration is between 1-3 years and salary is commensurate with qualifications.

Interested candidates should apply online:

https://jobs.ncsu.edu/applicants/Central?quickFind=89348

AA/EEO. In addition, NC State welcomes all persons without regard to sexual orientation.

James LeBeau
Assistant Professor
Department of Materials Science & Engineering
North Carolina State University
jmlebeau-at-ncsu.edu






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From: vish.bhakthavatsalam-at-gmail.com
Date: Sat, 15 Jan 2011 09:08:13 -0600
Subject: [Microscopy] WWW:Position Open: Microscopist with a phD in chemistry

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Email: vish.bhakthavatsalam-at-gmail.com
Name: Vishnupriya

Organization: Aditya Birla Science and Technology

Title-Subject: [Filtered] Microscopist with a phD in chemistry

Message: A minimum Ph.D. in Physical Sciences,
Analytical Chemistry, Material Science or related
discipline
Ô Experience
A minimum of 2-3 years of Hands Ůon instrument
experience in SEM-EDS, EBSD, TEM are required in
the industry. Experience with materials used in
the mining/metal industry is a bonus; A
background in mineralogy and hands on experience
in characterization techniques thereof (SEM-EDS,
EBSD, TEM, AFM, sputter coating, sample
preparation using ultra microtome) is required.
Experience in surface science processes and
techniques (e.g. AFM, STM) will be considered
especially advantageous.

Ô Mandatory skills
Good communication and presentation skills must
be evident; peer review publications in
international journals and presentations at
significant analytical conferences is a plus.
Erase this text and type your question here

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From: erwrigh-at-emory.edu
Date: Sat, 15 Jan 2011 09:08:40 -0600
Subject: [Microscopy] viaWWW: Postdoctoral Position Available

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Email: erwrigh-at-emory.edu
Name: Elizabeth Wright

Organization: Emory University

Title-Subject: [Filtered] Postdoctoral Position Available

Message: A postdoctoral fellow position is available immediately with
the Spearman and Wright laboratories at Emory University to study HIV
assembly and trafficking using fluorescence microscopy and cryo-EM
methods.

Motivated candidates with strong interests in HIV biology and
cryo-electron microscopy of complex molecular assemblies are
encouraged to apply. Research experience in the following area(s) is
desired: 1) retrovirology, 2) cryo-EM, and/or 3) fluorescence
microscopy.

Our laboratories are within the Division of Pediatric Infectious
Diseases and are fully equipped for all aspects of molecular
virology, molecular biology and protein biochemistry. The Spearman
lab has a Deltavision deconvolution microscopy station and a spinning
disk confocal system both equipped for live cell imaging, FRAP,
photoactivation, and now for cryo-imaging. The Wright lab has
complete access to a JEOL 2200FS 200 kV FEG TEM with an in-column
energy filter, Zernike phase plates, and high-resolution 4kx4k CCD
camera; a JEOL 1400 120 kV TEM with 2kx2k CCD camera; and an FEI
Vitrobot and a manual grid plunge freezer. Emory is a vibrant,
multidisciplinary campus with strong ties to Georgia Tech and other
universities within Georgia. Access to facilities in NMR, Mass
Spectrometry, and X-ray crystallography are available.

Applicants should include a cover letter describing research
experience and interests, curriculum vitae, and names and contact
information for three references.

Dr. Elizabeth R. Wright
Emory University
Department of Pediatrics
2015 Uppergate Drive, NE Room: 548
Atlanta, GA 30322
erwrigh-at-emory.edu
Website: http://electronmicroscopy.emory.edu

Applications will be reviewed as they are received and until the
position is filled.


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From: sarj0007-at-unf.edu
Date: Sat, 15 Jan 2011 13:02:12 -0600
Subject: [Microscopy] ci-46 incubator

Contents Retrieved from Microscopy Listserver Archives
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We have an old CI-46 water jacketed CO2 incubator that was donated years ago. Someone wants to brush the dust off and try using this because they want to avoid contaminating our main incubators with something they want to grow.

Our problem though is that we no longer have contact with the group that donated the incubator I think, and they didn't include a manual. Does anyone have a manual laying around or basic instructions for use? None of the ports on the backside are labeled anymore, and I don't know if American Scientific Products got absorbed by another company I could possibly call or did they just dissolve or do some other action causing it to be a problem tracking them down. I'm a youngling.

On the backside I just see three ports, one is the power, one is something electrical, and the other looks like a water siphon port. I'm assuming the nut on the front when you open the door might be the fill for the water jacket. We don't see where the CO2 is attached anywhere and their is a rather larger hole through the back of the chamber and we don't see anything that should fill that hole given to us along with the incubator. I guess they were afraid the cells might be scared of the dark?

Help in either getting us an old manual or contact for who might still be servicing these machines would be helpful. Not quite microscopy but assuming a few people are still around biology labs or maybe a colleague they know. Thanks and enjoy the weekend.

Off topic for this, thanks for those of you on the Ernst Ruska thread. Some interesting informative posts about history are always a good read.

-Jason Saredy
University of North Florida


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From: Michael.Cammer-at-med.nyu.edu
Date: Sun, 16 Jan 2011 09:30:29 -0600
Subject: [Microscopy] viaWWW: TIRF laser alignment question

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Email: Michael.Cammer-at-med.nyu.edu
Name: Michael Cammer

Organization: Skirball Langone NYU Med Center

Title-Subject: [Filtered] TIRF laser alignment question

Message: We have the Nikon TIRF system and have three laser lines
going into the TIRF arm via a single fiber. When we project through
the 100X objective through the sample onto the wall we see that the
lines go through the sample at different angles. (You can see a
picture of the projection at approx 45 degrees at
http://www.flickr.com/photos/mcammer/5359189090/ .) It is also
noticeable in the TIRF images that the field depth is different for
each wavelength. Is this unavoidable due to the different
wavelengths or is it possible to align the optics better so these
spots would be more coincident?

Thank you.

Sincerely,

Michael Cammer

Login Host: 96.246.240.120
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From: john.oreopoulos-at-utoronto.ca
Date: Sun, 16 Jan 2011 10:30:18 -0600
Subject: [Microscopy] Re: viaWWW: TIRF laser alignment question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Michael,

That's a beautiful photograph of the unavoidable relationship between the angle of incidence and the wavelength of light used to illuminate the sample. It stems from the fact that the relative index of refraction of the glass-water interface is slightly different for for each wavelength. To ensure that the penetration depth is nearly equal for all laser lines, you would have to offset each laser beam to a different specific radial position on the back focal plane of the objective - and these relative spacings would change as well if you wanted a different penetration depth. You can work out what these spacings need to be by examining the basic equation for the TIRF penetration depth (you have to assume you know the wavelength dependent index of refraction of the sample though which ranges from 1.33 to 1.38) and you'll find that they are on the order of a few tens of micrometers depending on the specific penetration depth you desire (see http://micro.magnet.fsu.edu/primer/techniques/fluorescence/tirf/tirfintro.html). There's a nice little ImageJ plugin that can be used:

http://rsbweb.nih.gov/ij/plugins/tirf/index.html

John Oreopoulos
Research Assistant
Spectral Applied Research
9078 Leslie Street, Unit 11
Richmond Hill, Ontario
Canada


On 2011-01-16, at 10:37 AM, Michael.Cammer-at-med.nyu.edu wrote:

}
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} Email: Michael.Cammer-at-med.nyu.edu
} Name: Michael Cammer
}
} Organization: Skirball Langone NYU Med Center
}
} Title-Subject: [Filtered] TIRF laser alignment question
}
} Message: We have the Nikon TIRF system and have three laser lines
} going into the TIRF arm via a single fiber. When we project through
} the 100X objective through the sample onto the wall we see that the
} lines go through the sample at different angles. (You can see a
} picture of the projection at approx 45 degrees at
} http://www.flickr.com/photos/mcammer/5359189090/ .) It is also
} noticeable in the TIRF images that the field depth is different for
} each wavelength. Is this unavoidable due to the different
} wavelengths or is it possible to align the optics better so these
} spots would be more coincident?
}
} Thank you.
}
} Sincerely,
}
} Michael Cammer
}
} Login Host: 96.246.240.120
} ---------------------------------------------------------------------------
}
} ==============================Original Headers==============================
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From: vray-at-partbeamsystech.com
Date: Mon, 17 Jan 2011 21:19:19 -0600
Subject: [Microscopy] Help with Noran/Voyager EDX in Florida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear John,

Thanks for the reply. Reading it and the referenced websites jogged my memory.

A few years ago we were having problems with the first commercial Olympus TIRF system because we could not get consistent evanescent waves with the one angle adjustment with the laser lines we had from 405 to 568 nm that were delivered via a single fiber (it was worse when we later added a 633 or 638 nm laser). I suggested we pump each laser in through a separate path that could be angled independently. We didn't build it, but I think Olympus now sells a TIRF system that does this.

Another issue is that when I first heard about TIRF maybe 15 years ago, it was introduced as a ring illumination at the outer edge of the back aperture, not as a single point or crescent at the periphery on only one side. A ring, or at least a series of points around the periphery, seems like a better way to provide a uniform field due to aberrations from coherent light in the imperfect optics. Any thought on this?

Sincerely,

Michael

_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270


________________________________________
X-from: John Oreopoulos [john.oreopoulos-at-utoronto.ca]
Sent: Sunday, January 16, 2011 11:30 AM
To: Cammer, Michael; Microscopy-at-microscopy.com

Dear Listers,

I am posting this on the request of Bill Caufman from Intersil Company
in Palm Bay, Florida. Bill needs help with reviving an EDX system with
"Voyager" electronics made by Noran and needs technical assistance.

If someone can provide technical help or even on-site assistance for
this kind of EDX system in Palm Bay Florida - please respond directly to
Bill at

BCAUFFMA-at-intersil.com

Thank you very much beforehand,
--
Valery Ray
=================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844 USA
Phone: +1-978-296-5063
US Mobile: +1-978-305-0479
Skype: pbstmeo
E-mail: vray-at-partbeamsystech.com

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From: Janne.Hyoetylae-at-stud.unibas.ch
Date: Tue, 18 Jan 2011 07:38:24 -0600
Subject: [Microscopy] Re: TIRF laser alignment question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Michael,


On Sun, 16 Jan 2011 19:19:44 +0100, {Michael.Cammer-at-med.nyu.edu} wrote:

} Another issue is that when I first heard about TIRF maybe 15 years ago,
} it was introduced as a ring illumination at the outer edge of the back
} aperture, not as a single point or crescent at the periphery on only one
} side. A ring, or at least a series of points around the periphery,
} seems like a better way to provide a uniform field due to aberrations
} from coherent light in the imperfect optics. Any thought on this?


Yes, you are right. See e.g. this paper about this very topic:
ÔĽŅFiolka, R., Belyaev, Y., Ewers, H., & Stemmer, A. (2008). Even
illumination in total internal reflection fluorescence microscopy using
laser light. Microscopy research and technique, 71(1), 45-50. doi:
10.1002/jemt.20527.

Cheers
Janne

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From: jehrman-at-mta.ca
Date: Tue, 18 Jan 2011 14:10:08 -0600
Subject: [Microscopy] Santovac 5? I need a few drops...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings listers,

The Deben stage controls on the SEM are getting a little stiff, and the
maintenance manual recommends Santovac 5 for lubrication. Too bad, I
don't have any around, and I don't relish the thought of forking over
several hundred dollars for an entire bottle just to use a miniscule
bit. Has anybody out there recently serviced a DP and have an "empty"
Santovac container that I could squeeze a few drops out of? I'll spring
for shipping, plus offer sacrifices to the gods of EM on your behalf for
good vibes and round electrons.

Thanks in advance,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Svent-Gyorgyi's Axiom:
Discovery consists of seeing what everybody
has seen and thinking what nobody thought.


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From: oshel1pe-at-cmich.edu
Date: Tue, 18 Jan 2011 14:18:36 -0600
Subject: [Microscopy] Re: Santovac 5? I need a few drops...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jim,

If you don't get any from others in the Great White North, let me
know, I can send you some.
If we can get it past NTSA and Customs and ...

Phil
Hm. "GWN" is somewhat more than the land of the Maple Leaf right now.

} Greetings listers,
}
} The Deben stage controls on the SEM are getting a little stiff, and the
} maintenance manual recommends Santovac 5 for lubrication. Too bad, I
} don't have any around, and I don't relish the thought of forking over
} several hundred dollars for an entire bottle just to use a miniscule
} bit. Has anybody out there recently serviced a DP and have an "empty"
} Santovac container that I could squeeze a few drops out of? I'll spring
} for shipping, plus offer sacrifices to the gods of EM on your behalf for
} good vibes and round electrons.
}
} Thanks in advance,
}
} Jim
}
} --
}
} James M. Ehrman
} Digital Microscopy Facility
} Mount Allison University
} 63B York St.
} Sackville, NB E4L 1G7
} CANADA
}
} phone: 506-364-2519
} fax: 506-364-2505
} email: jehrman-at-mta.ca
} www: http://www.mta.ca/dmf
}
} Svent-Gyorgyi's Axiom:
} Discovery consists of seeing what everybody
} has seen and thinking what nobody thought.

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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5, 25 -- Subject: Re: [Microscopy] Santovac 5? I need a few drops...
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From: joelsheffield-at-gmail.com
Date: Tue, 18 Jan 2011 16:20:08 -0600
Subject: [Microscopy] Ernst Ruska

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In 1963, ten years after the papers were publlished, I joined the Lab
of Dan Moore, at Rockefeller. That lab, which had been Porter's until
he moved to Harvard, was located in the basement of Smith Hall, and
actually extended under 68th street. At any rate, I learned microtomy
on one of the mechanical advance microtomes that were built in the
machine shop --I still have it--, and there was also a thermal advance
version. The thermal advancement was provided by an incandescent
light bulb suspended over the cutting arm. You advanced the block by
flashing the light. As you might expect, the area around the
microtome was forbidden to others while it was in use. Any air
currents would disturb the whole process.

Joel

On Thu, Jan 13, 2011 at 5:26 PM, {NEERAJG-at-clemson.edu} wrote:
}
}
}
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}
} So I did a little digging on who reported thermal advance in ultramicrotomes first (not which commercial instruments came first) the original paper by Keith Porter and J. Blum published in 1953, A study in Microtomy for electron Microscopy, The Anatomical Record Volume 117, Issue 4, pages 685-709, December 1953, in it Porter and Blum describe two new mechanisms, one is a thermal expansion and the other is mechanical advancement (I have a PDF of this paper if anyone is interested). Here is an excerpt from the Summary of this paper
}
} 'Two new microtomes capable of cutting serial sections as thin as 25-50 mu are described. All moving parts in these instruments are supported in unlubricated pivots and the specimen is taken past the cutting edge only on the cutting stroke. These two features more than any others seem responsible for the successful performance of these microtomes. In one instrument, the prototype, the block is advanced to the knife by thermal expansion. In the other, the advancement is controlled mechanically.'
}
} Interestingly enough, Fritiof Sjostrand's paper, A new microtome for ultrathin sectioning for high resolution microscopy, 1953 Experientia 9:114-121 (note the paper also published in 1953) says 'The microtome reported on in this paper represents a further development of the microtome designed by Porter' (http://www.springerlink.com/content/y3267k5456j11117/).
}
} Also found an interesting book, Picture Control: The Electron Microscope and the Transformation of Biology in America by Nicolas Rasmussen, has some interesting history on this matter (see preview, http://books.google.com/books?id=rwC5QiqLS44C&pg=PA127&lpg=PA127&dq=Sjostrand+a+new+ultramicrotome&source=bl&ots=HCTXlObRJ-&sig=G46YfqxY8lrFtBptZDgmxIDbxuA&hl=en&ei=cHAvTfLRBsKSgQfMo-xa&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCwQ6AEwAg#v=onepage&q&f=false
}
}
} Pretty Interesting!
}
} Best,
}
} Neeraj.
}
}
} Neeraj V. Gohad, Ph.D.
} Postdoctoral Fellow
} Okeanos Research Group
} Department of Biological Sciences
} 132 Long Hall
} Clemson University
} Clemson,SC-29634
} Phone: 864-656-3597
} Fax: 864-656-0435
}
} Website: http://www.clemson.edu/okeanos
}
}
}
}
}
}
}
}
}
}
}
} X-from: Paul Hazelton [mailto:hazeltn-at-cc.umanitoba.ca]
} Sent: Wednesday, January 12, 2011 4:09 PM
} To: Neeraj Gohad
} Subject: Re: [Microscopy] Ernst Ruska
}
} Neeraj et al
}
} To follow up on what Caroline said, thermal advance was first brought out by LKB, and I believe was developed by Sjostrand.
}
} Regards the Nobel Prize.† Story I heard but could never get confirmed was that the failure to give Ruska the prize before the 1980's started as political, proceeded to oversight, and in the end became an embarrassment.
}
} Ernst Ruska remained in Germany during WWII, and worked with the German war effort.† Please remember that one of the major uses of the microscope in Germany and the US was in the development of the atomic bomb.† Because of this, in the time leading up to, during and immediately after the war giving the prize to someone from Germany was not very acceptable.† This was the story to me in 1969, when I first started doing EM.† Ruska was, of course still alive, but as told, would not be a candidate for the prize.
}
} Over the ensuing years Ruska sort of got forgotten.† Then came the development of Scanning Tunneling EM.† In 1986, when the Academy was considering the award of the Prize in Physics to Binnig and Rohrer they realized, much to many people's chagrin, that Ruska had never been recognized.† Whatever his personal feelings may have been, his speech was gracious, and only said:
}
} "Here, I only want to emphasize my impression that the scanning tunnel electron microscopy of Gerd Binnig and Heinrich Rohrer has obviously been accepted much faster by scientific colleagues than electron microscopy fifty years ago."† (Ernst Ruska, Nobel Banquet Speech, December 10, 1986)
}
} Sadly, the failure to act in a more timely fashion meant that others such as Hans Busch and Max Knoll (the only names that jump to mind just now) did not also receive the recognition they deserved.
}
} Politics in the award of the Prize in all fields has been a long tradition.† It still carries on.† Witness the award of the Prize in Peace in 2009 to Barrack Obama, and the award of the Prize in Peace to Henry Kissinger in 1973, without recognition of Nixon, who, for all the wrong things he did, directed the negotiations which lead to the end of the Viet Nam war and ultimately deserved to share in this recognition.† And that is a† heck of a statement for someone considered to be politically to the left of centre.
}
} Paul Hazelton
}
} --
}
} Paul R. Hazelton, PhD
} Gastroenteric Diseases Study Group
} University of Manitoba
} Department of Medical Microbiology
} 511 Basic Medical Sciences Building
} 730 William Avenue
} Winnipeg, Manitoba, Canada, R3E 3J7
} e-mail: † paul_hazelton-at-umanitoba.ca
} Phone: † † † 204-789-3313 (w)
} † † † † † † † † 204-489-6924 (h)
} Cell: † † † † †204-781-6982
} Fax: † † † † †204-789-3926
}
}
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--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


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From: jehrman-at-mta.ca
Date: Wed, 19 Jan 2011 07:17:06 -0600
Subject: [Microscopy] Thanks! Re: Santovac 5? I need a few drops...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to all who replied with offers for some Santovac 5. I possibly
have a very local source, plus some other possibilities, so others who
were thinking of helping out are off the hook. Promised sacrifices to
the EM gods have already been offered. Just waiting now for the smoke to
clear...

As usual, the Listserv is an invaluable go-to source for all sorts of
goofy problems, esp. encountered in the wilds of the Great White North.

Cheers,

Jim


--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Directions: That useless little piece of paper,
containing incomprehensible language, usually
written in Spanish and Japanese, that one refers to,
only after botching the construction of the
over-priced and (also usually) useless item that
one found so necessary to own, only that very morning.


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From: Frank_Karl-at-lincolnelectric.com
Date: Wed, 19 Jan 2011 07:37:23 -0600
Subject: [Microscopy] EDS line scans and time on my hands

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I’ve been thinking about EDS line scans and I’m attempting to maximize the
data I collect.

I run at 20 KV on iron samples so my electron interaction range is between
1.5 to 2.3um (Berthe or K-O range calculations). According to data
provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at
700x. So right away I see if each image pixel represents a data point, I’m
sampling about 2 pixel volumes per data point. Looking for a sudden and
sharp change in element concentration seem difficult, but wait there’s
more. Let us introduce another plot complication.

My customers don’t want to scan the entire image, tooo much data…

So I scan a smaller section of image described above and instruct the
computer to take 512 data points along a line that might only be 400
(visual estimate from screen) image pixels long.

So… Should I reduce the image resolution so that each image pixel will be
one data point and scan the entire image? Would it be better to lower the
magnification so the image pixels are larger (say 1.2um ). I could go to
100x and 512 horizontal image pixel so each image pixel is 2.3um?

I’m looking for slight changes in element concentration at grain boundaries
and precipitates and of course I want to produce the most meaningful data
possible. I’m running at conditions that suggest my electron interactive
volume is larger than my spatial resolution as to produce ‚Äúcontinuous‚ÄĚ
data.

Or am I just over thinking the process?

Any suggestion or comments would be welcome

Frank
Lincoln Electric Company
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From: DOrloff-at-ascb.org
Date: Wed, 19 Jan 2011 08:25:59 -0600
Subject: [Microscopy] Cell Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Interested in Cell Microscopy?

Join the LinkedIn Group at http://www.linkedin.com/groupRegistration?gid=3733425

And check out The Cell: An Image Library at www.cellimagelibrary.org.

David

David Orloff
Manager, Image Library
The American Society for Cell Biology
8120 Woodmont Avenue, Suite 750
Bethesda, MD 20814-2762
T: 301-347-9300/Direct: 301-347-9305
F: 301-347-9310
E-mail:† dorloff-at-ascb.org
Web site: www.ascb.org
The Cell: An Image LibraryT: www.cellimagelibrary.org




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From: jehrman-at-mta.ca
Date: Wed, 19 Jan 2011 08:33:21 -0600
Subject: [Microscopy] source of small amounts of Santovac 5

Contents Retrieved from Microscopy Listserver Archives
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For those who may find themselves in my predicament, Bart has small
quantities of Santovac 5 available:

Jim,

I have Santovac 5 in small glass vials. 30 drops for $10.

You might try just brushing out the lead screws with acetone and a paper
towel guard to collect the spray. That will disperse any residual
coagulated Santovac 5.

Though I've been on this list for almost its entire life, I can no longer
post to the group since I can't get through the spam filter.

You might consider posting this e-mail to the group.

Bart Cannon
Cannon Microprobe
1041 NE 100th
Seattle, WA 98125
206 522 9233


--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Directions: That useless little piece of paper,
containing incomprehensible language, usually
written in Spanish and Japanese, that one refers to,
only after botching the construction of the
over-priced and (also usually) useless item that
one found so necessary to own, only that very morning.


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From: patpxs-at-gmail.com
Date: Wed, 19 Jan 2011 11:07:36 -0600
Subject: [Microscopy] Fuji Pictrography 3000

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Hello Listers,

We are trying to revive a Fuji Pitctrography 3000. We have the SCSI
to USB adapters but the pc we have still does not see that the printer
is there. We are using a pc with the Vista opreating system (don't
start with the comments about that!) and the Fuji uses Photoshop
plug-ins.

Any suggestions as to what to try next to get this printer up and runing?

Thanks in advance.

Paula

--
Paula Sicurello, HTL (ASCP)
Supervisor, Electron Microscope Laboratory
Duke University Health System
Rm.#M251 Duke South, Green Zone
Durham, North Carolina 27710
P: †919.684.2091


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From: ewestbrook-at-vsu.edu
Date: Wed, 19 Jan 2011 14:32:23 -0600
Subject: [Microscopy] viaWWW: How do I remove front panels on Hitachi TM-1000

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Email: ewestbrook-at-vsu.edu
Name: Edwina Westbrook

Organization: Virginia State University

Title-Subject: [Filtered] How do I remove front panels on Hitachi TM-1000

Message: Hello All,
I need to remove the front panels on the Hitachi TM-1000 Tabletop
SEM. Has anyone done this? If so, please respond. This is not
easy! I want to have the turbo molecular pump serviced.
Thanks!
Winnie Westbrook, M.Ed.
Electron Microscopy Research Lab
Virginia State University
Petersburg, VA 23806
804-524-5659

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From: anthonyribaudo3-at-gmail.com
Date: Wed, 19 Jan 2011 14:32:53 -0600
Subject: [Microscopy] viaWWW: SEM imaging of wax

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Email: anthonyribaudo3-at-gmail.com
Name: Anthony Ribaudo

Organization: TRI Princeton

Title-Subject: [Filtered] SEM imaging of wax

Message: Can anyone suggest guidelines for imaging a paraffin wax
coating on a metal or polymeric substrate via FESEM. The plan is to
detect the wax layer that is thought to be several hundred nanometers
thick? Are there any waxes that would be more stable to image under
the electron beam than paraffin wax?

Anthony Ribaudo
TRI Princeton

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From: Fengxia.Liang-at-med.nyu.edu
Date: Wed, 19 Jan 2011 14:33:35 -0600
Subject: [Microscopy] Postdoc/research associate position available at NYU School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A postdoc/research associate position supported by a newly renewed 5-year
NIH grant is available immediately in the Department of Biochemistry, NYU
School of Medicine. The research of the position focuses on structural
studies, using cryo-electron microscopy and electron tomography, of
uro-epithelial apical membrane and its receptor function for uropathogenic
E. coli (UPEC) in urinary tract infection (UTI) (see lab webpage:
http://kong.med.nyu.edu). Experience in electron microscopy and image
processing is desirable. Interested candidates should send CV and reference
information to xiangpeng.kong-at-med.nyu.edu.


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=================================



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From: z.zhou-at-lboro.ac.uk
Date: Wed, 19 Jan 2011 14:33:42 -0600
Subject: [Microscopy] viaWWW: TEM diffraction 3nm precipitates

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Email: z.zhou-at-lboro.ac.uk
Name: Zhou

Organization: Dept of Materials, Loughborough University, UK

Title-Subject: [Filtered] TEM diffraction 3nm precipitates

Message: Greetings listers,

IŪm studying crystal structure of some
carbides/nitrides precipitates in steel. The
precipitates are 2-5nm size in a C replica TEM
specimen, V-, Cr-, and Nb-rich by STEM/EDX.

How can I get electron diffraction or crystal
structure information of these fine precipitates?

I tried on a TecnaiF20 TEM using nanoprobe spot
7, focused on a small particle, then diffraction,
most of the time I got amorphous rings of C
support, no obvious diffraction discs. STEM mode
did indicate some C contamination on the session.
Assuming minimal C contamination, is it possible
that this method can give me some sort of
convergence beam electron diffraction pattern,
which may help me determine the crystal structure
of the precipitates?

IŪm also open to other methods.

Your advice is highly appreciated.

Zhou

Dr Zhaoxia Zhou
Dept of Materials
Loughborough University
Loughborough, UK
LE11 3TU


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From: sbarlow-at-sciences.sdsu.edu
Date: Wed, 19 Jan 2011 14:34:21 -0600
Subject: [Microscopy] viaWWW: color Laser printers

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Email: sbarlow-at-sciences.sdsu.edu
Name: Steve Barlow

Organization: SDSU EM Facility

Title-Subject: [Filtered] color Laser printers

Message: Hello All

Our general lab printer, a monochrome HP 2100, is on its last legs
and needs to be replaced, preferably with a color, duplex capable
printer.

We are looking at the Ricoh C430dn or the HP CP4525dn. We use the
lab printer for copy images, documentation of images. student
notebooks, etc.

I have had lots of experience with the HP printers, but none with
the Ricoh. Can anyone give me insights into their own experiences
with these two printers or the companies? Any other recommendations
for a laser printer?

Thanks in advance

Steve

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11, 22 -- Subject: viaWWW: color Laser printers
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From: dsherman-at-purdue.edu
Date: Wed, 19 Jan 2011 15:36:50 -0600
Subject: [Microscopy] Protocol for Norovirus TEM

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Hi all,

We will be imaging a fairly large number of norovirus samples using negative
staining. This is just for sample screening to document the presence or
absence of the virus.

The samples could be sent in fresh but I thought it might be better to fix
them prior to shipping so that they are not a health hazard. That might also
allow us to store them unfrozen for an extended period of time (months) with
reduced chance of deterioration or contamination.

Does anyone have a validated protocol that they are willing to share? I
used to know someone at the CDC who did TEM but no longer have that contact.

Thanks,
Debby
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy/



==============================Original Headers==============================
7, 28 -- From dsherman-at-purdue.edu Wed Jan 19 15:36:50 2011
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7, 28 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
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From: abel.orellana-at-sydney.edu.au
Date: Wed, 19 Jan 2011 15:43:17 -0600
Subject: [Microscopy] viaWWW: University of Sydney - Career Opportunity ACMM

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Email: abel.orellana-at-sydney.edu.au
Name: Abel Orellana

Organization: University of Sydney

Title-Subject: [Filtered] University of Sydney - Career Opportunity

Message: GENERAL OFFICE ADMINISTRATOR
THE AUSTRALIAN CENTRE FOR MICROSCOPY AND MICROANALYSIS (ACMM)
REFERENCE NO. 2615 /0810

Ô Be part of the largest Microscopy Facility of its type in Australia
Ô Opportunity to work on newly emerging microscopy techniques
Ô Remuneration package: $80,157

The University of Sydney is Australia's premier
University with an outstanding global reputation
for academic and research excellence, and employs
over 6,800 permanent staff supporting over 46,000
students.

The Australian Centre for Microscopy and
Microanalysis (ACMM) aims to provide leadership
in the development of innovation and ingenuity in
Australian science and engineering. The CentreŪs
vision is to be a world-leading facility for
modern microscopy and microanalysis, offering
premier instruments, services and training to
researchers from across Sydney and around
Australia, and providing strong leadership in the
national and international characterisation
communities.

We are currently seeking an experienced
Administrator to provide effective front desk and
administrative support to the main office of the
ACMM. Ideally suited to someone with an interest
in science, this is an excellent opportunity to
assist in the co-ordination of research
administration actives within research services.

You will be responsible for the professional
maintenance of the ACMM front desk, while being
first point-of contact for all queries. As such
it is imperative that you have excellent
communication and problem solving skills. The
position will see you co-ordinate and improve the
ACMM administration procedures including but not
limited to, arranging new user meetings; liaising
with users and visitors to the Centre in terms of
access; coordinating inductions for new staff and
students and providing support to the maintenance
of the CentreŪs website. Your demonstrated
ability to adapt to change and willingness to
undertake new tasks as the need arises will be
essential.

We are looking for a committed individual who
will thrive in a busy and changing environment.
To succeed, you will have previous experience
working in a busy office environment, existing
high level IT skills including creation of
spreadsheets, experience drafting and editing
reports along with experience producing high
level meeting minutes. You must also have
strong development and project management skills
and the ability to communicate with a diverse
range of individuals. Previous experience in a
Tertiary environment will be highly regarded.

The position is continuing subject to the
completion of a satisfactory probation period for
new appointees. Membership of a University
approved superannuation scheme is a condition of
employment for new appointees.

Remuneration package: a competitive remuneration
package is available of $80,157 (consisting of a
base salary $67,734, leave loading and up to 17%
employerŪs contribution to superannuation).

All applications must be submitted via The
University of Sydney careers website. Visit
sydney.edu.au/positions and search by the
reference number for more information and to
apply.


CLOSING DATE: 31 January 2011

The University is an Equal Opportunity employer
committed to equity, diversity and social
inclusion. Applications from equity target groups
and women are encouraged.


The University reserves the right not to proceed with any appointment.


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==============================Original Headers==============================
22, 24 -- From zaluzec-at-microscopy.com Wed Jan 19 15:43:16 2011
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22, 24 -- To: microscopy-at-microscopy.com
22, 24 -- From: abel.orellana-at-sydney.edu.au (by way of MicroscopyListserver)
22, 24 -- Subject: viaWWW: University of Sydney - Career Opportunity ACMM
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From: abel.orellana-at-sydney.edu.au
Date: Wed, 19 Jan 2011 15:44:11 -0600
Subject: [Microscopy] viaWWW: University of Sydney - Career Opportunity- SEM Specialist

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Email: abel.orellana-at-sydney.edu.au
Name: Abel Orellana

Organization: University of Sydney

Title-Subject: [Filtered] University of Sydney - Career Opportunity

Message: SCANNING ELECTRON MICROSCOPY SPECIALIST
THE AUSTRALIAN CENTRE FOR MICROSCOPY AND MICROANALYSIS (ACMM)
REFERENCE NO. 4273 /1210

Ô Be part of the largest Microscopy Facility of its type in Australia
Ô Opportunity to work on newly emerging microscopy techniques
Ô Remuneration package: $80,157

The University of Sydney is Australia's premier
University with an outstanding global reputation
for academic and research excellence, and employs
over 6,800 permanent staff supporting over 46,000
students.

The Australian Centre for Microscopy and
Microanalysis (ACMM) aims to provide leadership
in the development of innovation and ingenuity in
Australian science and engineering. The CentreŪs
vision is to be a world-leading facility for
modern microscopy and microanalysis, offering
premier instruments, services and training to
researchers from across Sydney and around
Australia, and providing strong leadership in the
national and international characterisation
communities.

As the Scanning Electron Microscopy Specialist
you will provide instruction, training and
support to users of the Centre's analytical
scanning electron microscope facilities including
primarily the needs of users in specimen
preparation, microscopy, microanalysis,
image/data analysis and interpretation. You will
have demonstrated expertise and skills in
specimen preparation techniques relevant to
scanning electron microscopy (SEM) and associated
techniques and skills in one or more of the
following: focussed ion beam milling (FIB)
techniques; environmental SEM (ESEM), cryo-SEM
imaging; In-Situ SEM instrumentation; Electron
Backscattered Diffraction (EBSD); quantitative
image analysis; X-ray microanalysis, X-ray
mapping; Mineral phase mapping

To succeed, you will possess an Honours degree in
science or engineering whereas a doctoral degree
with a high SEM component will be highly
advantageous. You will be a self motivated
individual with exceptional scientific
communication skills and a demonstrated ability
to relate to students, users, academic and
researchers at all levels. Experience in running
and working in a multi-user laboratory and
experience with the instruction of SEM techniques
is desirable.

The position is full-time, fixed term for three
years subject to the completion of a satisfactory
probation period for new appointees with the
possibility of further offer of employment.
Membership of a University approved
superannuation scheme is a condition of
employment for new appointees. Visa sponsorship
and relocation assistance may be offered to
suitable overseas applicants. A wide range of
employment benefits are also available, including
Living Away From Home Allowance (LAFHA).

Remuneration package: a competitive remuneration
package is available of $80,157 (consisting of a
base salary $67,734, leave loading and up to 17%
employerŪs contribution to superannuation).

All applications must be submitted via The
University of Sydney careers website. Visit
sydney.edu.au/positions and search by the
reference number for more information and to
apply.

CLOSING DATE: 31 January 2011

The University is an Equal Opportunity employer
committed to equity, diversity and social
inclusion. Applications from equity target groups
and women are encouraged.

The University reserves the right not to proceed with any appointment.


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==============================Original Headers==============================
19, 24 -- From zaluzec-at-microscopy.com Wed Jan 19 15:44:10 2011
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19, 24 -- To: microscopy-at-microscopy.com
19, 24 -- From: abel.orellana-at-sydney.edu.au (by way of MicroscopyListserver)
19, 24 -- Subject: viaWWW: University of Sydney - Career Opportunity- SEM Specialist
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From: abel.orellana-at-sydney.edu.au
Date: Wed, 19 Jan 2011 15:45:53 -0600
Subject: [Microscopy] viaWWW: University of Sydney - Career Opportunity-AMMRF

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Email: a.o.orellana
Name: Abel Orellana

Organization: University of Sydney

Title-Subject: [Filtered] University of Sydney - Career Opportunity

Message: MULTIMEDIA COMMUNICATIONS OFFICER
AUSTRALIAN MICROSCOPY & MICROSCOPY RESEARCH FACILITY (AMMRF)
REFERENCE NO. 4239 /1210

Ô Collaborate with AMMRF partners
Ô Opportunity to work with a variety of Media Communications
Ô Remuneration package: $80,157
Ô
The University of Sydney is Australia's premier
University with an outstanding global reputation
for academic and research excellence, and employs
over 6,800 permanent staff supporting over 46,000
students.

The ACMM provides leadership in the development
and application of advanced microscopy and
microanalysis techniques that support innovation
in Australian science and engineering. The centre
incorporates a substantial research portfolio, is
a node of the ARC Centre if Excellence for Design
in Light Metals and is the headquarters for the
AMMRF, The AMMRF is AustraliaŪs national research
facility for the characterisation of materials
through macro, meso, nano and atomic length
scales by means of advanced microscopy and
microanalysis. The facility is a joint venture of
eight universities, which forms a grid of
microscopy facilities with strategic links to six
other laboratories throughout Australia.

As the Multimedia Communications Officer you will
collaborate with AMMRF partners as well as teams
within the University of Sydney, to facilitate
and produce high-quality communications and
promotional outcomes. This will include the
creation, design and production of relevant
materials utilising a variety of media such as
written documents, presentations and the AMMRF
and ACMM websites. You will also assist in the
organisation and management of major events and
brand implementation for the AMMRF and the ACMM.

To be successful you must have previous
experience in the production of high quality
print and digital communications as well as the
ability to draft, edit and analyse print for
reports, publications and presentations. Your
experience and knowledge working with web
authoring, web design and graphic design software
as well your exceptional working knowledge of
Adobe Creative Suite Premium and Microsoft Office
is essential, as is the ability to build and
maintain effective working relationships.
Previous experience working in a scientific
organisation will be highly regarded.

The position is for 2.5 years, fixed term
subject to the completion of a satisfactory
probation period for new appointees with the
possibility of further offer of employment.
Membership of a University approved
superannuation scheme is a condition of
employment for new appointees.

Remuneration package: a competitive remuneration
package is available of $80,157 (consisting of a
base salary $67,734 leave loading and up to 17%
employerŪs contribution to superannuation).

All applications must be submitted via The
University of Sydney careers website. Visit
sydney.edu.au/positions and search by the
reference number for more information and to
apply.

CLOSING DATE: 31 January 2011

The University is an Equal Opportunity employer
committed to equity, diversity and social
inclusion. Applications from equity target groups
and women are encouraged.

Appointment is on merit; as women are
under-represented at this employment level
suitably qualified women are encouraged to apply.

The University reserves the right not to proceed with any appointment.


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==============================Original Headers==============================
19, 24 -- From zaluzec-at-microscopy.com Wed Jan 19 15:45:52 2011
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19, 24 -- To: microscopy-at-microscopy.com
19, 24 -- From: abel.orellana-at-sydney.edu.au (by way of MicroscopyListserver)
19, 24 -- Subject: viaWWW: University of Sydney - Career Opportunity-AMMRF
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From: rfoley-at-uab.edu
Date: Wed, 19 Jan 2011 16:50:11 -0600
Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work

Contents Retrieved from Microscopy Listserver Archives
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Email: rfoley-at-uab.edu
Name: Robin Foley

Organization: UAB

Title-Subject: [Filtered] Environmental SEM for Biological Work

Message: Is there heavy use of ESEM for biological SEM? What is
commonly done on biological ESEM samples? We're looking at
purchasing an environmental SEM and the sales rep. tells us we will
need a Peltier stage to cool the sample to look at wet samples.
Unfortunately, the maximum sample size for the Peltier stage is 3 mm
across. This seems tiny to me for an SEM. Are there many biological
ESEM applications that don't require the sample to be wet?

Thanks,

Robin Foley (who spends most of her SEM time looking at metals,
ceramics and polymers!)




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11, 22 -- Date: Wed, 19 Jan 2011 16:50:07 -0600
11, 22 -- To: microscopy-at-microscopy.com
11, 22 -- From: rfoley-at-uab.edu (by way of MicroscopyListserver)
11, 22 -- Subject: viaWWW: Environmental SEM for Biological Work
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From: David.Llewellyn-at-anu.edu.au
Date: Wed, 19 Jan 2011 17:02:00 -0600
Subject: [Microscopy] viaWWW: Tenupol-2 Electropolisher

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Email: David.Llewellyn-at-anu.edu.au
Name: David Llewellyn

Organization: Australian National University

Title-Subject: [Filtered] Tenupol-2 Electropolisher

Message: Am after some information re the Tenupol-2 and associated
Polypower Power Supply best of all would be off-line with somebody
who has one of these, thanks, David.

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==============================Original Headers==============================
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6, 22 -- Subject: viaWWW: Tenupol-2 Electropolisher
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From: rfklie-at-uic.edu
Date: Wed, 19 Jan 2011 17:18:47 -0600
Subject: [Microscopy] STEM post-doc position at UIC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

*Post-Doctoral Research Associate Position in Aberration-Corrected STEM*

* *

A post-doctoral research associate position is available in the
Nanoscale Physics Group at the University of Illinois at Chicago to work
on the application of atomic-resolution Z-contrast and annular
bright-field imaging, as well as electron energy-loss spectroscopy
(EELS). The successful candidate will work with our new JEOL ARM200CF,
an aberration-corrected cold-field emission STEM with sub-Ň and sub-eV
resolution, equipped with several in-situ holders.

Candidates should have a Ph.D. in Physics, Materials Science or related
disciplines. The position requires extensive experience in transmission
electron microscopy and electron energy-loss spectroscopy. Experience
working with aberration-corrected scanning transmission electron
microscopy is also preferred.

This postdoctoral position is available immediately, will be renewable
on an annual basis, and is anticipated for at least two years. The
position is open until it is filled.

Interested candidates can apply by email by sending a cover letter, CV
with a complete list of publications, and contact information of 3
professional references to:

Professor Robert F Klie

Department of Physics

University of Illinois at Chicago

845 W Taylor Street, M/C 273

Chicago, IL 60607

email: rfklie-at-uic.edu

--
Robert F. Klie, PhD
Assistant Professor
University of Illinois at Chicago
Department of Physics
Chicago, IL 60607
Tel: 312-996-6064
Fax: 312-996-9016

Editor
Journal of Undergraduate Research
Website {http://jur.phy.uic.edu}

President
Midwest Microscopy and Microanalysis Society (M^3 S)
Website {http://midwestmicroscopy.org}

==============================Original Headers==============================
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From: joelsheffield-at-gmail.com
Date: Wed, 19 Jan 2011 23:47:27 -0600
Subject: [Microscopy] Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

It is certainly clear that there are many interesting stories --some
urban legends, and some insights into the minds of those that founded
the discipline of thin section electron microscopy. During the mid
'70's, I taught an EM course at Rutgers, Camden. In the basement,
along with an RCA EMU-2 (!) was a thermal advance microtome that I was
told was developed by Sjostrand. This thing consisted of a disk-like
plate, about 4-5 inches in diameter, with an eccentrically mounted arm
that stuck out of the plate. The arm had a chuck for a tissue sample,
and a heating coil. The plate itself was mounted in a frame and
lubricated with oil --the plate acted like a large oil bearing. The
plate was linked to a motor on the wall by a long V belt (to minimize
vibration), and the entire plate rotated, bringing the block past the
knife on each rotation. The sections that resulted tended to be arc
shaped. We got it working, but it was a terrifying thing to see. I
wonder if anyone in this group remembers this type of machine
--assuming that my description is comprehensible.

The other microtome that I used, and really admire, was the Huxley
microtome, originally sold by Cambridge, and ultimately marketed by
LKB. Although it used a mechanical advance, all of the movements of
the cutting arm were made by bending sheets of spring steel, so that
there were no surfaces that moved against each other. Moreover, the
cutting stroke was controlled by an oil-filled damper, to minimize
chatter. --a remarkable and stable machine.

Now, the urban legend. I had heard that a group (unnamed) was having
a vigorous discussion about the best way to strop a steel knife so as
to get the best edge for cutting thin sections. How many strokes, in
which direction, which side of the blade, etc. During the discussion,
a bottle of Coke was knocked off the table and broke into many pieces.
The story goes that one, or another, of these people took up one of
the shards, and suggested that it would make a good knife edge.
Verification anyone?

Joel

--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs

==============================Original Headers==============================
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5, 30 -- Subject: Microtome History - was "Ernst Ruska"
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From: nizets2-at-yahoo.com
Date: Thu, 20 Jan 2011 02:23:23 -0600
Subject: [Microscopy] Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This urban legend differs slightly according to the origin of the narrator:

An american talks about a bottle of coke.
A french talks about a bottle of ricard
A belgian talks about a flask of beer
An† italian talks about a bottle of wine
A german talks about a bottle of schnaps
A russian talks about a bottle of vodka.
A japanese talks about a bottle of sake

Actually it would be†interesting to test all of them to see which one†cuts
better.
Now you just need†convincing†words for your grant....

Stephane†




----- Original Message ----
X-from: "joelsheffield-at-gmail.com" {joelsheffield-at-gmail.com}
To: nizets2-at-yahoo.com
Sent: Thu, January 20, 2011 6:51:55 AM

It is certainly clear that there are many interesting stories --some
urban legends, and some insights into the minds of those that founded
the discipline of thin section electron microscopy.† During the mid
'70's, I taught an EM course at Rutgers, Camden.† In the basement,
along with an RCA EMU-2 (!) was a thermal advance microtome that I was
told was developed by Sjostrand.† This thing consisted of a disk-like
plate, about 4-5 inches in diameter, with an eccentrically mounted arm
that stuck out of the plate.† The arm had a chuck for a tissue sample,
and a heating coil.† The plate itself was mounted in a frame and
lubricated with oil --the plate acted like a large oil bearing.† The
plate was linked to a motor on the wall by a long V belt (to minimize
vibration), and the entire plate rotated, bringing the block past the
knife on each rotation.† The sections that resulted tended to be arc
shaped.† We got it working, but it was a terrifying thing to see.† I
wonder if anyone in this group remembers this type of machine
--assuming that my description is comprehensible.

The other microtome that I used, and really admire, was the Huxley
microtome, originally sold by Cambridge, and ultimately marketed by
LKB.† Although it used a mechanical advance, all of the movements of
the cutting arm were made by bending sheets of spring steel, so that
there were no surfaces that moved against each other.† Moreover, the
cutting stroke was controlled by an oil-filled damper, to minimize
chatter. --a remarkable and stable machine.

Now, the urban legend.† I had heard that a group (unnamed) was having
a vigorous discussion about the best way to strop a steel knife so as
to get the best edge for cutting thin sections.† How many strokes, in
which direction, which side of the blade, etc.† During the discussion,
a bottle of Coke was knocked off the table and broke into many pieces.
The story goes that one, or another, of these people took up one of
the shards, and suggested that it would make a good knife edge.
Verification anyone?

Joel

--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs

==============================Original Headers==============================
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5, 30 -- Subject: Microtome History - was "Ernst Ruska"
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From: W.Muss-at-salk.at
Date: Thu, 20 Jan 2011 04:47:01 -0600
Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Von: MuŖ Wolfgang
Gesendet: Donnerstag, 20. Jšnner 2011 11:46
An: 'joelsheffield-at-gmail.com'
Cc: 'microscopy-at-microscopy.com'
Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"


Joel wrote:
} Now, the urban legend.†
I had heard that a group (unnamed) was having a vigorous discussion about the best way to strop a steel knife so as to get the best edge for cutting thin sections.† How many strokes, in which direction, which side of the blade, etc.† During the discussion, a bottle of Coke was knocked off the table and broke into many pieces.†
The story goes that one, or another, of these people took up one of the shards, and suggested that it would make a good knife edge. Verification anyone? {

Joel


Good morning,
Dear Joel, dear all

I can't tell or verify the above mentioned "Coke-shard-story" but - indeed - I can add information as to the "knocked off table"-part....

I had always heared (personal informations from elder REICHERT-freaks and service people, from lectures/lecturers in the 70ies and 80ies as well as personally from H. Sitte himself) that the group around H. SITTE at REICHERT (now LEICA) in the late 1950ies/60ies was/were doing such experiments with {glass plates} .... which were thrown down to the floor... and one of them (it was Sitte himself, as he told me) picked up the shards evaluating the edges of "the most promising one" for sectioning purposes (who of that group had the "invention" to use glass as a} crude { knife (element) I unfortunately don't know.

By the way, digging a little bit on this in Google, I found one hint on a document in a German data bank at
==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.†

{Ein einfaches Ultramikrotom mit thermischem Vorschub} verŲffentlicht
("A simple ultramicrotome with thermal advance" published)
in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367

Note:† {Naturwissenschaften} is the former title of††††† Biomedical and Life Sciences† (SPRINGER)
Another source:† http://www.freepatentsonline.com/3828641.html:
United States Patent US3828641:

==} Apparatus for adjusting the elevation of a specimen in microtomes, particularly ultramicrotomes
Inventor:† Hellmuth Sitte, HOMBURG (not Humburg [as written in the patent document!]/Saar Germany)
Assignee:† C.Reichert Optische Werke AG, Vienna, Austria)
Filed:† Nov. 10,1972

Also see http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false
(From the book Michael J. Dykstra, Laura E. Reuss - 2003† (see page "History: in the text displayed
the Reichert OMU-1 (before 1964, perhaps see document above in {Naturwissenschaften} 1955) from Sitte described as having thermal advance, Sitte was never happy with, since 1964: OMU-2 with thermal advance....

This just for your pleasure....
(and I sure that searching for Sitte Hellmuth or Sitte and microtome will find some results more on that interesting and exciting "history" of ultramicrotomy advances.....

Best wishes and regards,
have a good and successful rest of the week and a beautiful weekend,

Wolfgang MUSS (Ph.D.)
EM-Lab Gen.Hosp. SALK-LKH
SALZBURG
AUSTRIA







} -----UrsprŁngliche Nachricht-----
} Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} Gesendet: Donnerstag, 20. Jšnner 2011 06:51
} An: MuŖ Wolfgang
} Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
}
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}
} It is certainly clear that there are many interesting stories --some
} urban legends, and some insights into the minds of those that founded
} the discipline of thin section electron microscopy.
} During the mid '70's, I taught an EM course at Rutgers, Camden.
} In the basement, along with an RCA EMU-2 (!) was a thermal advance
} microtome that I was told was developed by Sjostrand.
} This thing consisted of a disk-like plate, about 4-5 inches in
} diameter, with an eccentrically mounted arm that stuck out of the
} plate.† The arm had a chuck for a tissue sample, and a heating coil.
} The plate itself was mounted in a frame and lubricated with oil --the
} plate acted like a large oil bearing.† The plate was linked to a motor
} on the wall by a long V belt (to minimize vibration), and the entire
} plate rotated, bringing the block past the knife on each rotation.† The
} sections that resulted tended to be arc shaped.† We got it working, but
} it was a terrifying thing to see.† I wonder if anyone in this group
} remembers this type of machine -- assuming that my description is
} comprehensible.
}
} The other microtome that I used, and really admire, was the Huxley
} microtome, originally sold by Cambridge, and ultimately marketed by
} LKB.† Although it used a mechanical advance, all of the movements of
} the cutting arm were made by bending sheets of spring steel, so that
} there were no surfaces that moved against each other.† Moreover, the
} cutting stroke was controlled by an oil-filled damper, to minimize
} chatter. -- a remarkable and stable machine.
}
} Now, the urban legend.
} I had heard that a group (unnamed) was having a vigorous discussion
} about the best way to strop a steel knife so as to get the best edge
} for cutting thin sections.† How many strokes, in which direction, which
} side of the blade, etc.† During the discussion, a bottle of Coke was
} knocked off the table and broke into many pieces.
} The story goes that one, or another, of these people took up one of the
} shards, and suggested that it would make a good knife edge.
} Verification anyone?
}
} Joel
}
} --
} Joel B. Sheffield, Ph.D.
} Biology Department, Temple University
} 1900 North 12th Street
} Philadelphia, PA 19122
} jbs-at-temple.edu
} (215) 204 8839, fax (215) 204 0486
} http://astro.temple.edu/~jbs
}
} ==============================Original
} Headers==============================
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} 5, 30 -- Subject: Microtome History - was "Ernst Ruska"
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From: David.Patton-at-uwe.ac.uk
Date: Thu, 20 Jan 2011 05:52:55 -0600
Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter.

You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe.

Does anyone know if this means the sample can be any size one likes?

Dave

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Email: rfoley-at-uab.edu
Name: Robin Foley

Organization: UAB

Title-Subject: [Filtered] Environmental SEM for Biological Work

Message: Is there heavy use of ESEM for biological SEM? What is
commonly done on biological ESEM samples? We're looking at
purchasing an environmental SEM and the sales rep. tells us we will
need a Peltier stage to cool the sample to look at wet samples.
Unfortunately, the maximum sample size for the Peltier stage is 3 mm
across. This seems tiny to me for an SEM. Are there many biological
ESEM applications that don't require the sample to be wet?

Thanks,

Robin Foley (who spends most of her SEM time looking at metals,
ceramics and polymers!)




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From: lovett-at-tcnj.edu
Date: Thu, 20 Jan 2011 08:19:13 -0600
Subject: [Microscopy] Early microtomy--urban legend?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Fellow microscopists:

I was taught decades ago that early ultrathin sections were made in the
following manner:

A sheet of plate glass was dropped on the floor. The shard with the
best edge was selected and attached to the blade of a small window fan.
The resin-embedded specimen was attached to the tip of a soldering
iron. once everything was clamped and aligned, the fan was turned on,
the soldering iron was plugged in, and the section were collected in a
large pan of water held beneath the fan. (As the soldering iron heated,
the thermal expansion advanced the specimen into the glass blade.)
Sections of optimal thickness were selected from those floating in the
pan on the basis of the color refracted.

I love the story. Does anyone know whether this even approaches a true
story?

Thanks,

Don

P.S. Anyone still alive who has first-hand knowledge of this story
would be pretty old by now, but I thought that I would give this a shot.

--

Donald L. Lovett e-mail: lovett-at-tcnj.edu
Professor and Chairperson phone: 609-771-2876
Department of Biology fax: 609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ 08628-0718


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From: kenconverse-at-qualityimages.biz
Date: Thu, 20 Jan 2011 08:33:16 -0600
Subject: [Microscopy] viaWWW: SEM imaging of wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Anthony,
If you don't have a cold stage on the FESEM, I would suggest finding some
faithful old tungsten SEM that can stand the abuse of wax evaporating under
the beam. That will also go a long way towards staying in the good graces
of whoever is in charge of the FESEM.

If you have a cold stage, then others on the list can probably give you some
help on that score.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


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Email: anthonyribaudo3-at-gmail.com
Name: Anthony Ribaudo

Organization: TRI Princeton

Title-Subject: [Filtered] SEM imaging of wax

Message: Can anyone suggest guidelines for imaging a paraffin wax
coating on a metal or polymeric substrate via FESEM. The plan is to
detect the wax layer that is thought to be several hundred nanometers
thick? Are there any waxes that would be more stable to image under
the electron beam than paraffin wax?

Anthony Ribaudo
TRI Princeton

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From: PhillipsT-at-missouri.edu
Date: Thu, 20 Jan 2011 08:54:00 -0600
Subject: [Microscopy] Early microtomy--urban legend?

Contents Retrieved from Microscopy Listserver Archives
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I have heard all the microtome and glass knives stories also but have no evidence to their veracity. But I once was talking to Sanford Palay - an early pioneer in EM - and he told me how they used a rubber hammer to tap the outside of the TEM column to try and nudge the aperture into place. I can't imagine how tedious that must have been. Of course, the reward the pioneers all got from primitive microtomy and TEMs was that they got to discover amazing new things of a bigger magnitude than most of the incremental advances we all get to contribute to these days. But I think I would rather have done science then rather than now when high cost forces specialization and a greater proportion of time is devoted to finding funding rather than organelles. Tom

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu]
Sent: Thursday, January 20, 2011 8:20 AM
To: Phillips, Thomas E.

Fellow microscopists:

I was taught decades ago that early ultrathin sections were made in the
following manner:

A sheet of plate glass was dropped on the floor. The shard with the
best edge was selected and attached to the blade of a small window fan.
The resin-embedded specimen was attached to the tip of a soldering
iron. once everything was clamped and aligned, the fan was turned on,
the soldering iron was plugged in, and the section were collected in a
large pan of water held beneath the fan. (As the soldering iron heated,
the thermal expansion advanced the specimen into the glass blade.)
Sections of optimal thickness were selected from those floating in the
pan on the basis of the color refracted.

I love the story. Does anyone know whether this even approaches a true
story?

Thanks,

Don

P.S. Anyone still alive who has first-hand knowledge of this story
would be pretty old by now, but I thought that I would give this a shot.

--

Donald L. Lovett e-mail: lovett-at-tcnj.edu
Professor and Chairperson phone: 609-771-2876
Department of Biology fax: 609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ 08628-0718


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From: bkang-at-ufl.edu
Date: Thu, 20 Jan 2011 08:54:46 -0600
Subject: [Microscopy] Postdoctoral Research Associate Position available at the University of Florida

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

The University of Florida, College of Medicine, Department of Biochemistry and Molecular Biology seeks an enthusiastic Ph.D. level structural biologist, with experience in the area of cryo-electron microscopy and/or X-ray crystallography and image reconstruction to work on the structure of ssDNA virus proteins and intact capsids. The position will require knowledge of molecular biology, biochemistry, and structural biology approaches. Training can be provided, as needed, but a basic knowledge of the technical aspects of these approaches is required. In addition, projects in the lab often require collaborative effort, thus an ability/desire to work in a group setting is preferable. Salary will be negotiable and commensurate with experience. Interested applicants should send their Curriculum Vitae plus the names of three individuals who can provide a letter of reference to Dr. Mavis Agbandje-McKenna, Department of Biochemistry and Molecular Biology, 1600 SW Archer Road, PO
Box 100245, Gainesville, FL 32610-0245 or email to mckenna-at-ufl.edu . The University of Florida is an Equal Opportunity Employee.

Thanks,

Byung-Ho Kang, Ph.D.
Assistant Professor, Microbiology and Cell Science
Director, Electron Microscopy and Bioimaging Lab, Interdisciplinary Center for Biotechnology Research
University of Florida Gainesville, FL 32611
Tel: 352-846-0952
Fax: 352-392-5922



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From: nizets2-at-yahoo.com
Date: Thu, 20 Jan 2011 08:56:22 -0600
Subject: [Microscopy] viaWWW: SEM imaging of wax

Contents Retrieved from Microscopy Listserver Archives
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Just a question†disguised in an answer: wouldn't it be the right application†to
make a†replica?

Stephane


----- Original Message ----
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To: nizets2-at-yahoo.com
Sent: Wed, January 19, 2011 9:39:49 PM

This Question/Comment was submitted to the Microscopy Listserver
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Email: anthonyribaudo3-at-gmail.com
Name: Anthony Ribaudo

Organization: TRI Princeton

Title-Subject: [Filtered] SEM imaging of wax

Message: Can anyone suggest guidelines for imaging a paraffin wax
coating on a metal or polymeric substrate via FESEM. The plan is to
detect the wax layer that is thought to be several hundred nanometers
thick? Are there any waxes that would be more stable to image under
the electron beam than paraffin wax?

Anthony Ribaudo
TRI Princeton

† Login Host: 108.5.140.150
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From: paul_hazelton-at-umanitoba.ca
Date: Thu, 20 Jan 2011 09:36:45 -0600
Subject: [Microscopy] Early microtomy--urban legend?

Contents Retrieved from Microscopy Listserver Archives
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Forgot how much fun these discussions of the "old days" can be. At
Washington University there was an old Seimens microscope. At the time
I was training as a tech, and using the microscope was not an option.
However, the man who trained me, Charles Kuhn, told me that one of the
microscopes he had used, and I believe he was referring to the old
Seimens at WU, had to be aligned using a rubber mallet. Never did it
personally, but was regaled with stories by one who did.

paul
--
Paul R. Hazelton, PhD
Gastroenteric Diseases Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w)
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926


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From: oshel1pe-at-cmich.edu
Date: Thu, 20 Jan 2011 09:53:22 -0600
Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
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I know at least 2 old microscopists who made
glass knifes by breaking window glass - one of
them here at CMU. He went to construction sites
to get the broken windows.

I'm running down the reference and (I hope) an
image, but one of the early tries at
ultramicrotomy was sticking razor blades on a
centrifuge rotor, then mount the sections on the
tub, close the lid and turn on the centrifuge.
The sections where then picked up from inside the
tub, having been flung willy-nilly around the
inside.
(The image is used in the microtomy lecture to
convince the students thin sectioning could be a
lot worse than they think it is.)

Phil

} Von: MuĢ Wolfgang
} Gesendet: Donnerstag, 20. JĒnner 2011 11:46
} An: 'joelsheffield-at-gmail.com'
} Cc: 'microscopy-at-microscopy.com'
} Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
}
}
} Joel wrote:
} } Now, the urban legend.›
} I had heard that a group (unnamed) was having a
} vigorous discussion about the best way to strop
} a steel knife so as to get the best edge for
} cutting thin sections.› How many strokes, in
} which direction, which side of the blade, etc.›
} During the discussion, a bottle of Coke was
} knocked off the table and broke into many
} pieces.›
} The story goes that one, or another, of these
} people took up one of the shards, and suggested
} that it would make a good knife edge.
} Verification anyone? {
}
} Joel
}
}
} Good morning,
} Dear Joel, dear all
}
} I can't tell or verify the above mentioned
} "Coke-shard-story" but - indeed - I can add
} information as to the "knocked off
} table"-part....
}
} I had always heared (personal informations from
} elder REICHERT-freaks and service people, from
} lectures/lecturers in the 70ies and 80ies as
} well as personally from H. Sitte himself) that
} the group around H. SITTE at REICHERT (now
} LEICA) in the late 1950ies/60ies was/were doing
} such experiments with {glass plates} .... which
} were thrown down to the floor... and one of them
} (it was Sitte himself, as he told me) picked up
} the shards evaluating the edges of "the most
} promising one" for sectioning purposes (who of
} that group had the "invention" to use glass as
} a} crude { knife (element) I unfortunately don't
} know.
}
} By the way, digging a little bit on this in
} Google, I found one hint on a document in a
} German data bank at
} ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.›
}
} {Ein einfaches Ultramikrotom mit thermischem Vorschub} verĖffentlicht
} ("A simple ultramicrotome with thermal advance" published)
} in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
}
} Note:› {Naturwissenschaften} is the former title
} of››››› Biomedical and Life Sciences› (SPRINGER)
} Another source:› http://www.freepatentsonline.com/3828641.html:
} United States Patent US3828641:
}
} ==} Apparatus for adjusting the elevation of a
} specimen in microtomes, particularly
} ultramicrotomes
} Inventor:› Hellmuth Sitte, HOMBURG (not Humburg
} [as written in the patent document!]/Saar
} Germany)
} Assignee:› C.Reichert Optische Werke AG, Vienna, Austria)
} Filed:› Nov. 10,1972
}
} Also see
} http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false
} (From the book Michael J. Dykstra, Laura E.
} Reuss - 2003› (see page "History: in the text
} displayed
} the Reichert OMU-1 (before 1964, perhaps see
} document above in {Naturwissenschaften} 1955)
} from Sitte described as having thermal advance,
} Sitte was never happy with, since 1964: OMU-2
} with thermal advance....
}
} This just for your pleasure....
} (and I sure that searching for Sitte Hellmuth or
} Sitte and microtome will find some results more
} on that interesting and exciting "history" of
} ultramicrotomy advances.....
}
} Best wishes and regards,
} have a good and successful rest of the week and a beautiful weekend,
}
} Wolfgang MUSS (Ph.D.)
} EM-Lab Gen.Hosp. SALK-LKH
} SALZBURG
} AUSTRIA
}
}
}
}
}
}
}
} } -----Ursprłngliche Nachricht-----
} } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} } Gesendet: Donnerstag, 20. JĒnner 2011 06:51
} } An: MuĢ Wolfgang
} } Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
} }
} } -----------------------------------------------------------------------
} } -----
} } The Microscopy ListServer -- CoSponsor:› The Microscopy Society of
} } America
} } To› Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } -----
} }
} } It is certainly clear that there are many interesting stories --some
} } urban legends, and some insights into the minds of those that founded
} } the discipline of thin section electron microscopy.
} } During the mid '70's, I taught an EM course at Rutgers, Camden.
} } In the basement, along with an RCA EMU-2 (!) was a thermal advance
} } microtome that I was told was developed by Sjostrand.
} } This thing consisted of a disk-like plate, about 4-5 inches in
} } diameter, with an eccentrically mounted arm that stuck out of the
} } plate.› The arm had a chuck for a tissue sample, and a heating coil.
} } The plate itself was mounted in a frame and lubricated with oil --the
} } plate acted like a large oil bearing.› The plate was linked to a motor
} } on the wall by a long V belt (to minimize vibration), and the entire
} } plate rotated, bringing the block past the knife on each rotation.› The
} } sections that resulted tended to be arc shaped.› We got it working, but
} } it was a terrifying thing to see.› I wonder if anyone in this group
} } remembers this type of machine -- assuming that my description is
} } comprehensible.
} }
} } The other microtome that I used, and really admire, was the Huxley
} } microtome, originally sold by Cambridge, and ultimately marketed by
} } LKB.› Although it used a mechanical advance, all of the movements of
} } the cutting arm were made by bending sheets of spring steel, so that
} } there were no surfaces that moved against each other.› Moreover, the
} } cutting stroke was controlled by an oil-filled damper, to minimize
} } chatter. -- a remarkable and stable machine.
} }
} } Now, the urban legend.
} } I had heard that a group (unnamed) was having a vigorous discussion
} } about the best way to strop a steel knife so as to get the best edge
} } for cutting thin sections.› How many strokes, in which direction, which
} } side of the blade, etc.› During the discussion, a bottle of Coke was
} } knocked off the table and broke into many pieces.
} } The story goes that one, or another, of these people took up one of the
} } shards, and suggested that it would make a good knife edge.
} } Verification anyone?
} }
} } Joel
} }
} } --
} } Joel B. Sheffield, Ph.D.
} } Biology Department, Temple University
} } 1900 North 12th Street
} } Philadelphia, PA 19122
} } jbs-at-temple.edu
} } (215) 204 8839, fax (215) 204 0486
} } http://astro.temple.edu/~jbs

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


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From: PhillipsT-at-missouri.edu
Date: Thu, 20 Jan 2011 10:20:18 -0600
Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I just remembered seeing a print ad from some science journal published the 40's or 50's (before my time!) for an early ultramicrotome. It was a high speed motor spinning some type of blade. The concept was that the block was advanced into this buzzsaw and you were supposed to catch the sections flying off. At the time I think the view was that ultrathins could only be cut at high speed. The real kicker was the ad mentioned the motor was also suitable for use in centrifuges. Crazy. Tom


Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Thursday, January 20, 2011 9:54 AM
To: Phillips, Thomas E.

I know at least 2 old microscopists who made
glass knifes by breaking window glass - one of
them here at CMU. He went to construction sites
to get the broken windows.

I'm running down the reference and (I hope) an
image, but one of the early tries at
ultramicrotomy was sticking razor blades on a
centrifuge rotor, then mount the sections on the
tub, close the lid and turn on the centrifuge.
The sections where then picked up from inside the
tub, having been flung willy-nilly around the
inside.
(The image is used in the microtomy lecture to
convince the students thin sectioning could be a
lot worse than they think it is.)

Phil

} Von: MuĢ Wolfgang
} Gesendet: Donnerstag, 20. J"nner 2011 11:46
} An: 'joelsheffield-at-gmail.com'
} Cc: 'microscopy-at-microscopy.com'
} Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
}
}
} Joel wrote:
} } Now, the urban legend.›
} I had heard that a group (unnamed) was having a
} vigorous discussion about the best way to strop
} a steel knife so as to get the best edge for
} cutting thin sections.› How many strokes, in
} which direction, which side of the blade, etc.›
} During the discussion, a bottle of Coke was
} knocked off the table and broke into many
} pieces.›
} The story goes that one, or another, of these
} people took up one of the shards, and suggested
} that it would make a good knife edge.
} Verification anyone? {
}
} Joel
}
}
} Good morning,
} Dear Joel, dear all
}
} I can't tell or verify the above mentioned
} "Coke-shard-story" but - indeed - I can add
} information as to the "knocked off
} table"-part....
}
} I had always heared (personal informations from
} elder REICHERT-freaks and service people, from
} lectures/lecturers in the 70ies and 80ies as
} well as personally from H. Sitte himself) that
} the group around H. SITTE at REICHERT (now
} LEICA) in the late 1950ies/60ies was/were doing
} such experiments with {glass plates} .... which
} were thrown down to the floor... and one of them
} (it was Sitte himself, as he told me) picked up
} the shards evaluating the edges of "the most
} promising one" for sectioning purposes (who of
} that group had the "invention" to use glass as
} a} crude { knife (element) I unfortunately don't
} know.
}
} By the way, digging a little bit on this in
} Google, I found one hint on a document in a
} German data bank at
} ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.›
}
} {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht
} ("A simple ultramicrotome with thermal advance" published)
} in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
}
} Note:› {Naturwissenschaften} is the former title
} of››››› Biomedical and Life Sciences› (SPRINGER)
} Another source:› http://www.freepatentsonline.com/3828641.html:
} United States Patent US3828641:
}
} ==} Apparatus for adjusting the elevation of a
} specimen in microtomes, particularly
} ultramicrotomes
} Inventor:› Hellmuth Sitte, HOMBURG (not Humburg
} [as written in the patent document!]/Saar
} Germany)
} Assignee:› C.Reichert Optische Werke AG, Vienna, Austria)
} Filed:› Nov. 10,1972
}
} Also see
} http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false
} (From the book Michael J. Dykstra, Laura E.
} Reuss - 2003› (see page "History: in the text
} displayed
} the Reichert OMU-1 (before 1964, perhaps see
} document above in {Naturwissenschaften} 1955)
} from Sitte described as having thermal advance,
} Sitte was never happy with, since 1964: OMU-2
} with thermal advance....
}
} This just for your pleasure....
} (and I sure that searching for Sitte Hellmuth or
} Sitte and microtome will find some results more
} on that interesting and exciting "history" of
} ultramicrotomy advances.....
}
} Best wishes and regards,
} have a good and successful rest of the week and a beautiful weekend,
}
} Wolfgang MUSS (Ph.D.)
} EM-Lab Gen.Hosp. SALK-LKH
} SALZBURG
} AUSTRIA
}
}
}
}
}
}
}
} } -----Ursprłngliche Nachricht-----
} } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} } Gesendet: Donnerstag, 20. J"nner 2011 06:51
} } An: MuĢ Wolfgang
} } Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
} }
} } -----------------------------------------------------------------------
} } -----
} } The Microscopy ListServer -- CoSponsor:› The Microscopy Society of
} } America
} } To› Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } -----------------------------------------------------------------------
} } -----
} }
} } It is certainly clear that there are many interesting stories --some
} } urban legends, and some insights into the minds of those that founded
} } the discipline of thin section electron microscopy.
} } During the mid '70's, I taught an EM course at Rutgers, Camden.
} } In the basement, along with an RCA EMU-2 (!) was a thermal advance
} } microtome that I was told was developed by Sjostrand.
} } This thing consisted of a disk-like plate, about 4-5 inches in
} } diameter, with an eccentrically mounted arm that stuck out of the
} } plate.› The arm had a chuck for a tissue sample, and a heating coil.
} } The plate itself was mounted in a frame and lubricated with oil --the
} } plate acted like a large oil bearing.› The plate was linked to a motor
} } on the wall by a long V belt (to minimize vibration), and the entire
} } plate rotated, bringing the block past the knife on each rotation.› The
} } sections that resulted tended to be arc shaped.› We got it working, but
} } it was a terrifying thing to see.› I wonder if anyone in this group
} } remembers this type of machine -- assuming that my description is
} } comprehensible.
} }
} } The other microtome that I used, and really admire, was the Huxley
} } microtome, originally sold by Cambridge, and ultimately marketed by
} } LKB.› Although it used a mechanical advance, all of the movements of
} } the cutting arm were made by bending sheets of spring steel, so that
} } there were no surfaces that moved against each other.› Moreover, the
} } cutting stroke was controlled by an oil-filled damper, to minimize
} } chatter. -- a remarkable and stable machine.
} }
} } Now, the urban legend.
} } I had heard that a group (unnamed) was having a vigorous discussion
} } about the best way to strop a steel knife so as to get the best edge
} } for cutting thin sections.› How many strokes, in which direction, which
} } side of the blade, etc.› During the discussion, a bottle of Coke was
} } knocked off the table and broke into many pieces.
} } The story goes that one, or another, of these people took up one of the
} } shards, and suggested that it would make a good knife edge.
} } Verification anyone?
} }
} } Joel
} }
} } --
} } Joel B. Sheffield, Ph.D.
} } Biology Department, Temple University
} } 1900 North 12th Street
} } Philadelphia, PA 19122
} } jbs-at-temple.edu
} } (215) 204 8839, fax (215) 204 0486
} } http://astro.temple.edu/~jbs

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


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From: mmgraham06-at-gmail.com
Date: Thu, 20 Jan 2011 10:42:15 -0600
Subject: [Microscopy] viaWWW: Research Assistant Position at Yale University

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Email: mmgraham06-at-gmail.com
Name: Morven Graham

Organization: Yale School of Medicine

Title-Subject: [Filtered] Re:Research Assistant Position

Message: Hi Listeners

There is a current opening for a EM technician at Yale University Medical
School.

If you are interested you can check the site at

http://www.yale.edu/hronline/stars/application/external/index.html
STARS# 11657BR

Thanks

Morven Graham
CCMI, YSM
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New Haven
CT06511

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From: John.Mardinly-at-wdc.com
Date: Thu, 20 Jan 2011 11:10:26 -0600
Subject: [Microscopy] Early microtomy--urban legend?

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I had a Topcon 002B at Intel for many years for which use of a hammer was
required. The ion pump would periodically grow whiskers that resulted in
markedly increased ion current. The best way to get rid of them was to bang
on the pump with a hammer. I don't mean a rubber mallet either. To be fully
effective, this required a big iron hammer and hearing protection, as well
as thick skin to withstand the curious stares and complaints about the noise
and questions about one's sanity from fellow workers.

John Mardinly

Western Digital

-----Original Message-----
X-from: paul_hazelton-at-umanitoba.ca [mailto:paul_hazelton-at-umanitoba.ca]
Sent: Thursday, January 20, 2011 7:44 AM
To: John Mardinly

Forgot how much fun these discussions of the "old days" can be. At
Washington University there was an old Seimens microscope. At the time
I was training as a tech, and using the microscope was not an option.
However, the man who trained me, Charles Kuhn, told me that one of the
microscopes he had used, and I believe he was referring to the old
Seimens at WU, had to be aligned using a rubber mallet. Never did it
personally, but was regaled with stories by one who did.

paul
--
Paul R. Hazelton, PhD
Gastroenteric Diseases Study Group
University of Manitoba
Department of Medical Microbiology
511 Basic Medical Sciences Building
730 William Avenue
Winnipeg, Manitoba, Canada, R3E 3J7
e-mail: paul_hazelton-at-umanitoba.ca
Phone: 204-789-3313 (w)
204-489-6924 (h)
Cell: 204-781-6982
Fax: 204-789-3926


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From: joelsheffield-at-gmail.com
Date: Thu, 20 Jan 2011 11:31:19 -0600
Subject: [Microscopy] Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
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Indeed. We are in a building that was often broken into. Years ago,
I made it my business to collect the old door panes --they were a
tinted glass, about 1/4" thick, and had just the right temper to make
excellent knives.

Joel

On Thu, Jan 20, 2011 at 10:59 AM, {oshel1pe-at-cmich.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
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}
} I know at least 2 old microscopists who made
} glass knifes by breaking window glass - one of
} them here at CMU. He went to construction sites
} to get the broken windows.
}
} I'm running down the reference and (I hope) an
} image, but one of the early tries at
} ultramicrotomy was sticking razor blades on a
} centrifuge rotor, then mount the sections on the
} tub, close the lid and turn on the centrifuge.
} The sections where then picked up from inside the
} tub, having been flung willy-nilly around the
} inside.
} (The image is used in the microtomy lecture to
} convince the students thin sectioning could be a
} lot worse than they think it is.)
}
} Phil
}
} } Von: MuĢ Wolfgang
} } Gesendet: Donnerstag, 20. JĒnner 2011 11:46
} } An: 'joelsheffield-at-gmail.com'
} } Cc: 'microscopy-at-microscopy.com'
} } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
} }
} }
} } Joel wrote:
} } } Now, the urban legend.›
} } I had heard that a group (unnamed) was having a
} } vigorous discussion about the best way to strop
} } a steel knife so as to get the best edge for
} } cutting thin sections.› How many strokes, in
} } which direction, which side of the blade, etc.›
} } During the discussion, a bottle of Coke was
} } knocked off the table and broke into many
} } pieces.›
} } The story goes that one, or another, of these
} } people took up one of the shards, and suggested
} } that it would make a good knife edge.
} } Verification anyone? {
} }
} } Joel
} }
} }
} } Good morning,
} } Dear Joel, dear all
} }
} } I can't tell or verify the above mentioned
} } "Coke-shard-story" but - indeed - I can add
} } information as to the "knocked off
} } table"-part....
} }
} } I had always heared (personal informations from
} } elder REICHERT-freaks and service people, from
} } lectures/lecturers in the 70ies and 80ies as
} } well as personally from H. Sitte himself) that
} } the group around H. SITTE at REICHERT (now
} } LEICA) in the late 1950ies/60ies was/were doing
} } such experiments with {glass plates} .... which
} } were thrown down to the floor... and one of them
} } (it was Sitte himself, as he told me) picked up
} } the shards evaluating the edges of "the most
} } promising one" for sectioning purposes (who of
} } that group had the "invention" to use glass as
} } a} crude { knife (element) I unfortunately don't
} } know.
} }
} } By the way, digging a little bit on this in
} } Google, I found one hint on a document in a
} } German data bank at
} } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.›
} }
} } {Ein einfaches Ultramikrotom mit thermischem Vorschub} verĖffentlicht
} } ("A simple ultramicrotome with thermal advance" published)
} } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
} }
} } Note:› {Naturwissenschaften} is the former title
} } of››››› Biomedical and Life Sciences› (SPRINGER)
} } Another source:› http://www.freepatentsonline.com/3828641.html:
} } United States Patent US3828641:
} }
} } ==} Apparatus for adjusting the elevation of a
} } specimen in microtomes, particularly
} } ultramicrotomes
} } Inventor:› Hellmuth Sitte, HOMBURG (not Humburg
} } [as written in the patent document!]/Saar
} } Germany)
} } Assignee:› C.Reichert Optische Werke AG, Vienna, Austria)
} } Filed:› Nov. 10,1972
} }
} } Also see
} } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false
} } (From the book Michael J. Dykstra, Laura E.
} } Reuss - 2003› (see page "History: in the text
} } displayed
} } the Reichert OMU-1 (before 1964, perhaps see
} } document above in {Naturwissenschaften} 1955)
} } from Sitte described as having thermal advance,
} } Sitte was never happy with, since 1964: OMU-2
} } with thermal advance....
} }
} } This just for your pleasure....
} } (and I sure that searching for Sitte Hellmuth or
} } Sitte and microtome will find some results more
} } on that interesting and exciting "history" of
} } ultramicrotomy advances.....
} }
} } Best wishes and regards,
} } have a good and successful rest of the week and a beautiful weekend,
} }
} } Wolfgang MUSS (Ph.D.)
} } EM-Lab Gen.Hosp. SALK-LKH
} } SALZBURG
} } AUSTRIA
} }
} }
} }
} }
} }
} }
} }
} } } †-----Ursprłngliche Nachricht-----
} } } †Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} } } †Gesendet: Donnerstag, 20. JĒnner 2011 06:51
} } } †An: MuĢ Wolfgang
} } } †Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
} } }
} } } †-----------------------------------------------------------------------
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} } } †America
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} } }
} } } †It is certainly clear that there are many interesting stories --some
} } } †urban legends, and some insights into the minds of those that founded
} } } †the discipline of thin section electron microscopy.
} } } †During the mid '70's, I taught an EM course at Rutgers, Camden.
} } } †In the basement, along with an RCA EMU-2 (!) was a thermal advance
} } } †microtome that I was told was developed by Sjostrand.
} } } †This thing consisted of a disk-like plate, about 4-5 inches in
} } } †diameter, with an eccentrically mounted arm that stuck out of the
} } } †plate.› The arm had a chuck for a tissue sample, and a heating coil.
} } } †The plate itself was mounted in a frame and lubricated with oil --the
} } } †plate acted like a large oil bearing.› The plate was linked to a motor
} } } †on the wall by a long V belt (to minimize vibration), and the entire
} } } †plate rotated, bringing the block past the knife on each rotation.› The
} } } †sections that resulted tended to be arc shaped.› We got it working, but
} } } †it was a terrifying thing to see.› I wonder if anyone in this group
} } } †remembers this type of machine -- assuming that my description is
} } } †comprehensible.
} } }
} } } †The other microtome that I used, and really admire, was the Huxley
} } } †microtome, originally sold by Cambridge, and ultimately marketed by
} } } †LKB.› Although it used a mechanical advance, all of the movements of
} } } †the cutting arm were made by bending sheets of spring steel, so that
} } } †there were no surfaces that moved against each other.› Moreover, the
} } } †cutting stroke was controlled by an oil-filled damper, to minimize
} } } †chatter. -- a remarkable and stable machine.
} } }
} } } †Now, the urban legend.
} } } †I had heard that a group (unnamed) was having a vigorous discussion
} } } †about the best way to strop a steel knife so as to get the best edge
} } } †for cutting thin sections.› How many strokes, in which direction, which
} } } †side of the blade, etc.› During the discussion, a bottle of Coke was
} } } †knocked off the table and broke into many pieces.
} } } †The story goes that one, or another, of these people took up one of the
} } } †shards, and suggested that it would make a good knife edge.
} } } †Verification anyone?
} } }
} } } †Joel
} } }
} } } †--
} } } †Joel B. Sheffield, Ph.D.
} } } †Biology Department, Temple University
} } } †1900 North 12th Street
} } } †Philadelphia, PA 19122
} } } †jbs-at-temple.edu
} } } †(215) 204 8839, fax (215) 204 0486
} } } †http://astro.temple.edu/~jbs
}
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
} (989) 774-3576
}
}
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--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


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From: ehaller-at-health.usf.edu
Date: Thu, 20 Jan 2011 11:43:13 -0600
Subject: [Microscopy] Early microtomy--urban legend?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I can confirm the rubber mallet story for column alignment. I worked at Mellon Institute in Pittsburgh in the '70's. So did my father, Martin Haller, who taught me electron Microscopy. He worked there from the '60's to the '70's. We had several RCA electron microscopes that were purchased many years before I was trained in microscopy. My father used to align the lenses in the column of these microscopes with a rubber mallet. I took a couple of photos on these microscopes back in 1974. They shot glass plates with 3 exposures. After the 3 shots, you had to break vacuum, take out your plate, replace it and repump the scope to be able to take any more photos.

Ed

Edward Haller, Lab Manager
University of South Florida
Integrative Biology Department
Electron Microscopy Core
SCA 110
4202 East Fowler Avenue
Tampa, FL 33620
813-974-2676
ehaller-at-usf.edu
Office: LSA 119
________________________________________
X-from: PhillipsT-at-missouri.edu [PhillipsT-at-missouri.edu]
Sent: Thursday, January 20, 2011 10:06 AM
To: Haller, Edward

I have heard all the microtome and glass knives stories also but have no evidence to their veracity. But I once was talking to Sanford Palay - an early pioneer in EM - and he told me how they used a rubber hammer to tap the outside of the TEM column to try and nudge the aperture into place. I can't imagine how tedious that must have been. Of course, the reward the pioneers all got from primitive microtomy and TEMs was that they got to discover amazing new things of a bigger magnitude than most of the incremental advances we all get to contribute to these days. But I think I would rather have done science then rather than now when high cost forces specialization and a greater proportion of time is devoted to finding funding rather than organelles. Tom

Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: lovett-at-tcnj.edu [mailto:lovett-at-tcnj.edu]
Sent: Thursday, January 20, 2011 8:20 AM
To: Phillips, Thomas E.

Fellow microscopists:

I was taught decades ago that early ultrathin sections were made in the
following manner:

A sheet of plate glass was dropped on the floor. The shard with the
best edge was selected and attached to the blade of a small window fan.
The resin-embedded specimen was attached to the tip of a soldering
iron. once everything was clamped and aligned, the fan was turned on,
the soldering iron was plugged in, and the section were collected in a
large pan of water held beneath the fan. (As the soldering iron heated,
the thermal expansion advanced the specimen into the glass blade.)
Sections of optimal thickness were selected from those floating in the
pan on the basis of the color refracted.

I love the story. Does anyone know whether this even approaches a true
story?

Thanks,

Don

P.S. Anyone still alive who has first-hand knowledge of this story
would be pretty old by now, but I thought that I would give this a shot.

--

Donald L. Lovett e-mail: lovett-at-tcnj.edu
Professor and Chairperson phone: 609-771-2876
Department of Biology fax: 609-637-5118
The College of New Jersey
P.O. Box 7718
Ewing, NJ 08628-0718


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From: stefan.diller-at-t-online.de
Date: Thu, 20 Jan 2011 11:57:20 -0600
Subject: [Microscopy] viaWWW: SEM imaging of wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I had the same problem visualizing bee wax structures and not owning an ESEM ;-)

Solution: I put the structure on a large specimen mount, putting 2 bands of conductive adhesive tape left and right on top and put
it in my normal Pt-sputtercoater.
Concerning the heat during sputtering, I took off the sputter-head and added a second glass vessel (I took it from my
carbon-coater) on top of the first.
Did ca. 10-15 sputtering-runs with minimal current (10mA) and finally put it in the scope at 5-6kV.

See images at
http://www.electronmicroscopy.info/wax

It`s a bit of a crude solution but it works.
Concerning the thickness of your wax layers you may also try the same thing with an high-vac chromecoater...


Best wishes,
Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 20.01.11 16:00, schrieb nizets2-at-yahoo.com:
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} Just a question disguised in an answer: wouldn't it be the right application to
} make a replica?
}
} Stephane
}
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} ----- Original Message ----
} X-from: "anthonyribaudo3-at-gmail.com" {anthonyribaudo3-at-gmail.com}
} To: nizets2-at-yahoo.com
} Sent: Wed, January 19, 2011 9:39:49 PM
} Subject: [Microscopy] viaWWW: SEM imaging of wax
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} Email: anthonyribaudo3-at-gmail.com
} Name: Anthony Ribaudo
}
} Organization: TRI Princeton
}
} Title-Subject: [Filtered] SEM imaging of wax
}
} Message: Can anyone suggest guidelines for imaging a paraffin wax
} coating on a metal or polymeric substrate via FESEM. The plan is to
} detect the wax layer that is thought to be several hundred nanometers
} thick? Are there any waxes that would be more stable to image under
} the electron beam than paraffin wax?
}
} Anthony Ribaudo
} TRI Princeton
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From: William.F.Tivol-at-aero.org
Date: 01/19/2011 12:51 PM
Subject: [Microscopy] viaWWW: TEM diffraction 3nm precipitates

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Dear Zhou,
If possible, I would put in a very small SA aperture and try to
use a parallel beam to get Bragg spots. These precipitates are very
small, so you may need to take long exposures, which means that the
specimen and aperture positions must be very stable. In order to see
diffraction from the small particles, the diffraction from the much larger
surrounding area must be minimized (or subtracted out).
Yours,
Bill



X-from: z.zhou-at-lboro.ac.uk
To: William.F.Tivol-at-aero.org



Organization: Dept of Materials, Loughborough University, UK

Title-Subject: [Filtered] TEM diffraction 3nm precipitates

Message: Greetings listers,

IŪm studying crystal structure of some
carbides/nitrides precipitates in steel. The
precipitates are 2-5nm size in a C replica TEM
specimen, V-, Cr-, and Nb-rich by STEM/EDX.

How can I get electron diffraction or crystal
structure information of these fine precipitates?

I tried on a TecnaiF20 TEM using nanoprobe spot
7, focused on a small particle, then diffraction,
most of the time I got amorphous rings of C
support, no obvious diffraction discs. STEM mode
did indicate some C contamination on the session.
Assuming minimal C contamination, is it possible
that this method can give me some sort of
convergence beam electron diffraction pattern,
which may help me determine the crystal structure
of the precipitates?

IŪm also open to other methods.

Your advice is highly appreciated.

Zhou



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From: William.F.Tivol-at-aero.org
Date: 01/19/2011 05:45 AM
Subject: [Microscopy] EDS line scans and time on my hands

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Dear Frank,
The key is that you're "looking for slight changes in element
concentrations at grain boundaries and precipitates." In order best to
see these changes, the pixel size should be smaller than the
features--half the feature size guarantees that at least one pixel will be
entirely within the feature. If the pixel size is too large than you will
measure the average element concentrations over both the feature and the
surrounding area, which will make the apparent changes smaller than they
really are. I would suggest taking an image to locate the features, then
place the beam on each feature in turn to do the analysis. Also take a
spectrum of the area next to each feature. This should give you the best
chance of seeing differences and minimize the amount of data.
Yours,
Bill



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To: William.F.Tivol-at-aero.org






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I‚??ve been thinking about EDS line scans and I‚??m attempting to maximize
the
data I collect.

I run at 20 KV on iron samples so my electron interaction range is between
1.5 to 2.3um (Berthe or K-O range calculations). According to data
provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at
700x. So right away I see if each image pixel represents a data point,
I‚??m
sampling about 2 pixel volumes per data point. Looking for a sudden and
sharp change in element concentration seem difficult, but wait there‚??s
more. Let us introduce another plot complication.

My customers don‚??t want to scan the entire image, tooo much data‚?¶

So I scan a smaller section of image described above and instruct the
computer to take 512 data points along a line that might only be 400
(visual estimate from screen) image pixels long.

So‚?¶ Should I reduce the image resolution so that each image pixel will
be
one data point and scan the entire image? Would it be better to lower the
magnification so the image pixels are larger (say 1.2um ). I could go to
100x and 512 horizontal image pixel so each image pixel is 2.3um?

I‚??m looking for slight changes in element concentration at grain
boundaries
and precipitates and of course I want to produce the most meaningful data
possible. I‚??m running at conditions that suggest my electron
interactive
volume is larger than my spatial resolution as to produce ‚??continuous‚?Ě
data.

Or am I just over thinking the process?

Any suggestion or comments would be welcome

Frank
Lincoln Electric Company
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From: John.Mardinly-at-wdc.com
Date: Thu, 20 Jan 2011 12:33:11 -0600
Subject: [Microscopy] Re: Microtome History - was "Ernst Ruska"

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This sounds more like a food processor than a microtome!

John Mardinly

Western Digital


-----Original Message-----
X-from: PhillipsT-at-missouri.edu [mailto:PhillipsT-at-missouri.edu]
Sent: Thursday, January 20, 2011 8:30 AM
To: John Mardinly

I just remembered seeing a print ad from some science journal published the
40's or 50's (before my time!) for an early ultramicrotome. It was a high
speed motor spinning some type of blade. The concept was that the block was
advanced into this buzzsaw and you were supposed to catch the sections
flying off. At the time I think the view was that ultrathins could only be
cut at high speed. The real kicker was the ad mentioned the motor was also
suitable for use in centrifuges. Crazy. Tom


Thomas E. Phillips, Ph.D
Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://www.biotech.missouri.edu/mcc/

-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Thursday, January 20, 2011 9:54 AM
To: Phillips, Thomas E.

I know at least 2 old microscopists who made
glass knifes by breaking window glass - one of
them here at CMU. He went to construction sites
to get the broken windows.

I'm running down the reference and (I hope) an
image, but one of the early tries at
ultramicrotomy was sticking razor blades on a
centrifuge rotor, then mount the sections on the
tub, close the lid and turn on the centrifuge.
The sections where then picked up from inside the
tub, having been flung willy-nilly around the
inside.
(The image is used in the microtomy lecture to
convince the students thin sectioning could be a
lot worse than they think it is.)

Phil

} Von: Mu Wolfgang
} Gesendet: Donnerstag, 20. J"nner 2011 11:46
} An: 'joelsheffield-at-gmail.com'
} Cc: 'microscopy-at-microscopy.com'
} Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
}
}
} Joel wrote:
} } Now, the urban legend.
} I had heard that a group (unnamed) was having a
} vigorous discussion about the best way to strop
} a steel knife so as to get the best edge for
} cutting thin sections. How many strokes, in
} which direction, which side of the blade, etc.
} During the discussion, a bottle of Coke was
} knocked off the table and broke into many
} pieces.
} The story goes that one, or another, of these
} people took up one of the shards, and suggested
} that it would make a good knife edge.
} Verification anyone? {
}
} Joel
}
}
} Good morning,
} Dear Joel, dear all
}
} I can't tell or verify the above mentioned
} "Coke-shard-story" but - indeed - I can add
} information as to the "knocked off
} table"-part....
}
} I had always heared (personal informations from
} elder REICHERT-freaks and service people, from
} lectures/lecturers in the 70ies and 80ies as
} well as personally from H. Sitte himself) that
} the group around H. SITTE at REICHERT (now
} LEICA) in the late 1950ies/60ies was/were doing
} such experiments with {glass plates} .... which
} were thrown down to the floor... and one of them
} (it was Sitte himself, as he told me) picked up
} the shards evaluating the edges of "the most
} promising one" for sectioning purposes (who of
} that group had the "invention" to use glass as
} a} crude { knife (element) I unfortunately don't
} know.
}
} By the way, digging a little bit on this in
} Google, I found one hint on a document in a
} German data bank at
} ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.
}
} {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht
} ("A simple ultramicrotome with thermal advance" published)
} in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
}
} Note: {Naturwissenschaften} is the former title
} of Biomedical and Life Sciences (SPRINGER)
} Another source: http://www.freepatentsonline.com/3828641.html:
} United States Patent US3828641:
}
} ==} Apparatus for adjusting the elevation of a
} specimen in microtomes, particularly
} ultramicrotomes
} Inventor: Hellmuth Sitte, HOMBURG (not Humburg
} [as written in the patent document!]/Saar
} Germany)
} Assignee: C.Reichert Optische Werke AG, Vienna, Austria)
} Filed: Nov. 10,1972
}
} Also see
} http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+an
d+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl
=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CC
EQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false
} (From the book Michael J. Dykstra, Laura E.
} Reuss - 2003 (see page "History: in the text
} displayed
} the Reichert OMU-1 (before 1964, perhaps see
} document above in {Naturwissenschaften} 1955)
} from Sitte described as having thermal advance,
} Sitte was never happy with, since 1964: OMU-2
} with thermal advance....
}
} This just for your pleasure....
} (and I sure that searching for Sitte Hellmuth or
} Sitte and microtome will find some results more
} on that interesting and exciting "history" of
} ultramicrotomy advances.....
}
} Best wishes and regards,
} have a good and successful rest of the week and a beautiful weekend,
}
} Wolfgang MUSS (Ph.D.)
} EM-Lab Gen.Hosp. SALK-LKH
} SALZBURG
} AUSTRIA
}
}
}
}
}
}
}
} } -----Ursprngliche Nachricht-----
} } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} } Gesendet: Donnerstag, 20. J"nner 2011 06:51
} } An: Mu Wolfgang
} } Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
} }
} } -----------------------------------------------------------------------
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} }
} } It is certainly clear that there are many interesting stories --some
} } urban legends, and some insights into the minds of those that founded
} } the discipline of thin section electron microscopy.
} } During the mid '70's, I taught an EM course at Rutgers, Camden.
} } In the basement, along with an RCA EMU-2 (!) was a thermal advance
} } microtome that I was told was developed by Sjostrand.
} } This thing consisted of a disk-like plate, about 4-5 inches in
} } diameter, with an eccentrically mounted arm that stuck out of the
} } plate. The arm had a chuck for a tissue sample, and a heating coil.
} } The plate itself was mounted in a frame and lubricated with oil --the
} } plate acted like a large oil bearing. The plate was linked to a motor
} } on the wall by a long V belt (to minimize vibration), and the entire
} } plate rotated, bringing the block past the knife on each rotation. The
} } sections that resulted tended to be arc shaped. We got it working, but
} } it was a terrifying thing to see. I wonder if anyone in this group
} } remembers this type of machine -- assuming that my description is
} } comprehensible.
} }
} } The other microtome that I used, and really admire, was the Huxley
} } microtome, originally sold by Cambridge, and ultimately marketed by
} } LKB. Although it used a mechanical advance, all of the movements of
} } the cutting arm were made by bending sheets of spring steel, so that
} } there were no surfaces that moved against each other. Moreover, the
} } cutting stroke was controlled by an oil-filled damper, to minimize
} } chatter. -- a remarkable and stable machine.
} }
} } Now, the urban legend.
} } I had heard that a group (unnamed) was having a vigorous discussion
} } about the best way to strop a steel knife so as to get the best edge
} } for cutting thin sections. How many strokes, in which direction, which
} } side of the blade, etc. During the discussion, a bottle of Coke was
} } knocked off the table and broke into many pieces.
} } The story goes that one, or another, of these people took up one of the
} } shards, and suggested that it would make a good knife edge.
} } Verification anyone?
} }
} } Joel
} }
} } --
} } Joel B. Sheffield, Ph.D.
} } Biology Department, Temple University
} } 1900 North 12th Street
} } Philadelphia, PA 19122
} } jbs-at-temple.edu
} } (215) 204 8839, fax (215) 204 0486
} } http://astro.temple.edu/~jbs

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576


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From: tina-at-pbrc.hawaii.edu
Date: Thu, 20 Jan 2011 12:34:03 -0600
Subject: [Microscopy] Re: Early microtomy--urban legend?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, All-

One of the hospitals here in Honolulu had an old RCA TEM that had a rubber
mallet in the alignment kit. They kept that old scope going until the
early 1980s, I believe, with frequent visits from an aged service
technician. It was a mechanical wonder.

I also heard of many of the early iterations of ultramicrotome, possibly
at the knee of Caroline Schooley, from whom I learned TEM in 1976. One
version I heard was mounting a block on the blade of a fan and running it
past a stationary knife at high speed, using the position of venetian
blinds on a sunny day as thickness control. One had to search around the
room for the sections. I had to make knives from plate glass for a couple
of years, and I might as well have given up on the pliers and just thrown
the glass on the floor...

Aloha,
Tina

Twenty to thirty foot waves predicted on the North Shore today.

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: swatkins-at-pitt.edu
Date: Thu, 20 Jan 2011 13:01:00 -0600
Subject: [Microscopy] Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If you check out the Glauert texts, in the volume by norma Reid and julian Beesley (pub 1991), they talk about the old high speed microtomes apparently razor blades were rotated at 10k rpm (the refs are Obrien et al 1943, and Fullam et al 1946, the Obrien paper was actually in Science vol 98, 455-456). For fun I made a similar device out of a dremel and a plastic salad bowl, the dremel cut the sections which flew off and stuck to the inner surface of the plastic bowl. I then filled it with water and the sections (at least some of them) floated off. The thickness was completely random, and the bowl size a bit enormous and not stationary.. think collecting sections from the surface of the Atlantic... but they were useable.. sort of... Obviously no serial sections. The other thing is that I was using contemporary resin technology, not available 65 years ago when this work was done, but I guess at the "cutting edge" (pun intended)
S

Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu


-----Original Message-----
X-from: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
Sent: Thursday, January 20, 2011 12:37 PM
To: Watkins, Simon C

Indeed. We are in a building that was often broken into. Years ago,
I made it my business to collect the old door panes --they were a
tinted glass, about 1/4" thick, and had just the right temper to make
excellent knives.

Joel

On Thu, Jan 20, 2011 at 10:59 AM, {oshel1pe-at-cmich.edu} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} I know at least 2 old microscopists who made
} glass knifes by breaking window glass - one of
} them here at CMU. He went to construction sites
} to get the broken windows.
}
} I'm running down the reference and (I hope) an
} image, but one of the early tries at
} ultramicrotomy was sticking razor blades on a
} centrifuge rotor, then mount the sections on the
} tub, close the lid and turn on the centrifuge.
} The sections where then picked up from inside the
} tub, having been flung willy-nilly around the
} inside.
} (The image is used in the microtomy lecture to
} convince the students thin sectioning could be a
} lot worse than they think it is.)
}
} Phil
}
} } Von: MuĢ Wolfgang
} } Gesendet: Donnerstag, 20. J"nner 2011 11:46
} } An: 'joelsheffield-at-gmail.com'
} } Cc: 'microscopy-at-microscopy.com'
} } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
} }
} }
} } Joel wrote:
} } } Now, the urban legend.›
} } I had heard that a group (unnamed) was having a
} } vigorous discussion about the best way to strop
} } a steel knife so as to get the best edge for
} } cutting thin sections.› How many strokes, in
} } which direction, which side of the blade, etc.›
} } During the discussion, a bottle of Coke was
} } knocked off the table and broke into many
} } pieces.›
} } The story goes that one, or another, of these
} } people took up one of the shards, and suggested
} } that it would make a good knife edge.
} } Verification anyone? {
} }
} } Joel
} }
} }
} } Good morning,
} } Dear Joel, dear all
} }
} } I can't tell or verify the above mentioned
} } "Coke-shard-story" but - indeed - I can add
} } information as to the "knocked off
} } table"-part....
} }
} } I had always heared (personal informations from
} } elder REICHERT-freaks and service people, from
} } lectures/lecturers in the 70ies and 80ies as
} } well as personally from H. Sitte himself) that
} } the group around H. SITTE at REICHERT (now
} } LEICA) in the late 1950ies/60ies was/were doing
} } such experiments with {glass plates} .... which
} } were thrown down to the floor... and one of them
} } (it was Sitte himself, as he told me) picked up
} } the shards evaluating the edges of "the most
} } promising one" for sectioning purposes (who of
} } that group had the "invention" to use glass as
} } a} crude { knife (element) I unfortunately don't
} } know.
} }
} } By the way, digging a little bit on this in
} } Google, I found one hint on a document in a
} } German data bank at
} } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.›
} }
} } {Ein einfaches Ultramikrotom mit thermischem Vorschub} ver-ffentlicht
} } ("A simple ultramicrotome with thermal advance" published)
} } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
} }
} } Note:› {Naturwissenschaften} is the former title
} } of››››› Biomedical and Life Sciences› (SPRINGER)
} } Another source:› http://www.freepatentsonline.com/3828641.html:
} } United States Patent US3828641:
} }
} } ==} Apparatus for adjusting the elevation of a
} } specimen in microtomes, particularly
} } ultramicrotomes
} } Inventor:› Hellmuth Sitte, HOMBURG (not Humburg
} } [as written in the patent document!]/Saar
} } Germany)
} } Assignee:› C.Reichert Optische Werke AG, Vienna, Austria)
} } Filed:› Nov. 10,1972
} }
} } Also see
} } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+and+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8&hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ved=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false
} } (From the book Michael J. Dykstra, Laura E.
} } Reuss - 2003› (see page "History: in the text
} } displayed
} } the Reichert OMU-1 (before 1964, perhaps see
} } document above in {Naturwissenschaften} 1955)
} } from Sitte described as having thermal advance,
} } Sitte was never happy with, since 1964: OMU-2
} } with thermal advance....
} }
} } This just for your pleasure....
} } (and I sure that searching for Sitte Hellmuth or
} } Sitte and microtome will find some results more
} } on that interesting and exciting "history" of
} } ultramicrotomy advances.....
} }
} } Best wishes and regards,
} } have a good and successful rest of the week and a beautiful weekend,
} }
} } Wolfgang MUSS (Ph.D.)
} } EM-Lab Gen.Hosp. SALK-LKH
} } SALZBURG
} } AUSTRIA
} }
} }
} }
} }
} }
} }
} }
} } } -----Ursprłngliche Nachricht-----
} } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} } } Gesendet: Donnerstag, 20. J"nner 2011 06:51
} } } An: MuĢ Wolfgang
} } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
} } }
} } } -----------------------------------------------------------------------
} } } -----
} } } The Microscopy ListServer -- CoSponsor:› The Microscopy Society of
} } } America
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} } }
} } } It is certainly clear that there are many interesting stories --some
} } } urban legends, and some insights into the minds of those that founded
} } } the discipline of thin section electron microscopy.
} } } During the mid '70's, I taught an EM course at Rutgers, Camden.
} } } In the basement, along with an RCA EMU-2 (!) was a thermal advance
} } } microtome that I was told was developed by Sjostrand.
} } } This thing consisted of a disk-like plate, about 4-5 inches in
} } } diameter, with an eccentrically mounted arm that stuck out of the
} } } plate.› The arm had a chuck for a tissue sample, and a heating coil.
} } } The plate itself was mounted in a frame and lubricated with oil --the
} } } plate acted like a large oil bearing.› The plate was linked to a motor
} } } on the wall by a long V belt (to minimize vibration), and the entire
} } } plate rotated, bringing the block past the knife on each rotation.› The
} } } sections that resulted tended to be arc shaped.› We got it working, but
} } } it was a terrifying thing to see.› I wonder if anyone in this group
} } } remembers this type of machine -- assuming that my description is
} } } comprehensible.
} } }
} } } The other microtome that I used, and really admire, was the Huxley
} } } microtome, originally sold by Cambridge, and ultimately marketed by
} } } LKB.› Although it used a mechanical advance, all of the movements of
} } } the cutting arm were made by bending sheets of spring steel, so that
} } } there were no surfaces that moved against each other.› Moreover, the
} } } cutting stroke was controlled by an oil-filled damper, to minimize
} } } chatter. -- a remarkable and stable machine.
} } }
} } } Now, the urban legend.
} } } I had heard that a group (unnamed) was having a vigorous discussion
} } } about the best way to strop a steel knife so as to get the best edge
} } } for cutting thin sections.› How many strokes, in which direction, which
} } } side of the blade, etc.› During the discussion, a bottle of Coke was
} } } knocked off the table and broke into many pieces.
} } } The story goes that one, or another, of these people took up one of the
} } } shards, and suggested that it would make a good knife edge.
} } } Verification anyone?
} } }
} } } Joel
} } }
} } } --
} } } Joel B. Sheffield, Ph.D.
} } } Biology Department, Temple University
} } } 1900 North 12th Street
} } } Philadelphia, PA 19122
} } } jbs-at-temple.edu
} } } (215) 204 8839, fax (215) 204 0486
} } } http://astro.temple.edu/~jbs
}
} --
} Philip Oshel
} Microscopy Facility Supervisor
} Biology Department
} 024C Brooks Hall
} Central Michigan University
} Mt. Pleasant, MI 48859
} (989) 774-3576
}
}
} ==============================Original Headers==============================
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--
Joel B. Sheffield, Ph.D.
Biology Department, Temple University
1900 North 12th Street
Philadelphia, PA 19122
jbs-at-temple.edu
(215) 204 8839, fax (215) 204 0486
http://astro.temple.edu/~jbs


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From: schooley-at-mcn.org
Date: Thu, 20 Jan 2011 13:05:12 -0600
Subject: [Microscopy] Re: Early microtomy--urban legend?

Contents Retrieved from Microscopy Listserver Archives
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} ----------------------------------------------------------------------------
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This whole discussion has been a "this is my life" for me. Sorry,
Tina - the fan thing wasn't mine. I went to work for Dorothy Pitelka
at U.C. Berkeley in '53, and our first attempts at thin sections were
with a metal-knife Spencer microtome with a modifying wedge in the
advance mechanism. Miserable. Then a MT-1 with glass knives; a
local window company made 1" strips from old windows for us.

The rubber mallet was used on RCA EMU-2 scopes to align the objective
aperture....

Caroline
--
Caroline Schooley
Project MICRO Coordinator
Microscopy Society of America
45301 Caspar Point Road, Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Project MICRO: http://www.microscopy.org/ProjectMICRO

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From: bigelow-at-umich.edu
Date: Thu, 20 Jan 2011 13:42:35 -0600
Subject: [Microscopy] RE:Early Micro

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Paul Hazelton's comments about knowing someone who claimed to have
used a rubber mallet to align a Siemens microscope, reminds me of my
own experience along that line. I took mu first electron
micrographs on an RCA EMB microscope in Robley Williams lab in the
Physics Department here at the University of Michigan. This was only
the second EM manufactured and sold by RCA. Robley had two of these
instruments, one which he reserved for his own use and one that he
allowed other 'qualified' persons to use. It was said that he
selected the best pole pieces and power supplies from the two
instruments and incorporated them to his own, and so he had one of
the best electron microscopes in the country at the time. I was
deemed a qualified user because I was studying under Professor L. O.
Brockway who was internationally famous for his work on determining
the structures of organic molecules by electron diffraction. Robley
was, of course, probably best known for developing the method of
shadow casting that was extensively used to enhance contrast in
micrographs of particulate materials and surface replicas. At that
time the tobacco mosaic virus, which have a beautiful long rod-like
structure, was a favorite specimen to use to demonstrate the
resolution of an EM. I remember a lecture on the merits of shadow
casting where Robley showed a micrograph of beautifully shadowed
long rod-like particles that everyone naturally assumed were tobacco
mosaic virus; then Robley showed a second picture at higher
magnification in which the particles could be clearly seen to be
Lucky Strike cigarettes.

Anyway, halfway through the days of my graduate study Robley left the
U of M, and his microscope was no longer available. To continue our
work Professor Brockway managed to arrange for me to us an RCA EM2
electron microscope in the laboratory of Professor Thomas Francis in
the School of Public Health (Prof Francis was famous for heading up
the studies that confirmed the effectiveness of the Salk polio
vaccine.) This instrument was a considerable improvement over the
EMB; however, here is where the hammer-for-alignment feature came in.
The condenser aperture on this model instrument did not have external
controls for centering the condenser aperture, The aperture was
merely a circular platinum disk with a small hole in the middle that
was mounted in a cavity in the top of the condenser pole piece, and
held in place with a retaining screw. Initially, the aperture had to
be centered by trial and error, which involved disassembling the top
of the column, removing the pole piece from the condenser lens,
moving the aperture a bit, and then reassembling the instrument. To
get a really good alignment of the aperture usually required several
cycles of this routine combined with considerable luck. It was thus
very tempting to work with dirty condenser apertures. FINALLY,
however, someone (and I don't know that I ever heard who the sainted
individual was) discovered that if the retaining screw was not
tightened securely, so that the aperture could move around a bit, the
aperture could be centered by tapping gently on the outside of the
column. I don't recall ever using a rubber mallet, but the end of the
handle of a screw driver did the job very well.
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

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From: bigelow-at-umich.edu
Date: Thu, 20 Jan 2011 14:05:08 -0600
Subject: [Microscopy] Hammering ion pumps

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dr. Mardinly's comments about using a hammer to dislodge whiskers
inside an ion pump leads me to point out that if the whiskers are
not too firmly established it is often possible to get rid of them by
turning the high voltage supply on for a few seconds three or four
times with the pressure in the pump above 1 Pa (10-2 Torr), If this
approach works it is gentler, and quieter, than the hammer method.
Incidentally, this method (but not the hammer method) and other
characteristics of ion pumps, are described on page 295 of my book,
'Vacuum Methods in Electron Microscopy'.

Good luck!
--
Wilbur C. Bigelow, Professor Emeritus
Materials Sci. & Engr., Univ. of Michigan
Ann Arbor, Michigan 48109-2136
e-mail: bigelow-at-umich.edu;
Fx:734-763-4788; Ph:734-975-0858
Address mail to: 2911 Whittier Court
Ann Arbor, MI 48104-6731

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From: wesaia-at-iastate.edu
Date: 01/19/2011 05:45 AM
Subject: [Microscopy] EDS line scans and time on my hands

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Frank, it is good to see someone putting some thought into the process. I have had numerous students/clients come to me and ask for an analysis with some particular parameters only for me to ask if they really wanted to invest that much time and money. (Maybe I shouldn't have asked. I could have just given them the bill telling them that I only did what they asked. Naahh.)

Your interaction volume estimate seems reasonable, but you may need to look into cutting your voltage to cut down the volume. I would think 15 kV or even 12 kV would still get you a good response for most K-lines and would really cut down the volume.

Like Bill said, you want to match the volume to the feature size. That is the bottom line.

You probably also want to match the pixel size to the volume, but there is something to be said for oversampling. Even though the volume will be broader than your pixel, a lot of the interaction helps as the core. Yes, you will get stray signals from the surrounding area. You won't be able to say that the volume does not contain this element or that, but you will stand a better chance at finding what is elevated at that location as opposed to stepping over a feature and missing it.

Now as to your specific numbers, let me examine them.

You say that your pixel size is 0.8 um and there are 1024 across the image, so your field of view is roughly 800 um. I generally calculate magnification against a standard width of 120 mm (the width of a Polaroid print). That gives me a magnification of 150x. Now, I suppose if your image was displayed 560 mm wide on a 22-inch wide screen, it would be accurate to state it was at 700x magnification. Otherwise, there may be something wrong in your setup. It would be worth checking. However, your calculations of 2.3 um pixels with 512 of them across a 100x image does make mathematical sense.

(BTW, I am an old fogey in wanting to reference everything to a 5-inch Polaroid print. It doesn't matter if it's a thumbnail of an image on the web or in a journal or if it's plastered across a billboard. I tend to count all pictures as 5 inches wide in my mind's eye. Therefore, I have my SEM setup to calculate that way.)

I would probably setup for about 0.5 to 1.0 um steps across the boundaries. I would cut that even finer at lower voltages. (Indeed, I have such a sample in my queue.) I don't normally setup my pixels as my step sizes. They don't have to match unless you are collecting a spectral image or an x-ray map. Then I would want to match my pixel to my interaction volume or a bit smaller to make sure I have sampled everything well when in hunting mode.

Spectral images or x-ray maps do take considerably more time to collect. In that sense, I am with Bill to recommend that you chose your points wisely. You could probably collect good data much faster. I am not against elemental maps. Sometimes they are the best way to make a point, but they usually require pretty marked changes in concentration to perceive a change in intensity. For general work, I restrict myself to manually placed points or to a small raster of points in a specific region of interest.

Not knowing how your precipitates (might) occur, I can't give you a particular recommendation. I would be imaging as well as I could, probably in BSE mode, looking for visible signs of the particles and then using EDS to identify and quantify the material.

Best wishes,
Warren S.

-----Original Message-----
X-from: William.F.Tivol-at-aero.org [mailto:William.F.Tivol-at-aero.org]
Sent: Thursday, January 20, 2011 12:31 PM

Dear Frank,
The key is that you're "looking for slight changes in element concentrations at grain boundaries and precipitates." In order best to see these changes, the pixel size should be smaller than the features--half the feature size guarantees that at least one pixel will be entirely within the feature. If the pixel size is too large than you will measure the average element concentrations over both the feature and the surrounding area, which will make the apparent changes smaller than they really are. I would suggest taking an image to locate the features, then place the beam on each feature in turn to do the analysis. Also take a spectrum of the area next to each feature. This should give you the best chance of seeing differences and minimize the amount of data.
Yours,
Bill
from: Frank_Karl-at-lincolnelectric.com

I've been thinking about EDS line scans and I'm attempting to maximize
The data I collect.

I run at 20 KV on iron samples so my electron interaction range is between
1.5 to 2.3um (Berthe or K-O range calculations). According to data
provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um at
700x. So right away I see if each image pixel represents a data point, I'm
sampling about 2 pixel volumes per data point. Looking for a sudden and
sharp change in element concentration seem difficult, but wait there's
more. Let us introduce another plot complication.

My customers don't want to scan the entire image, tooo much data!

So I scan a smaller section of image described above and instruct the
computer to take 512 data points along a line that might only be 400
(visual estimate from screen) image pixels long.

So... Should I reduce the image resolution so that each image pixel will be
one data point and scan the entire image? Would it be better to lower the
magnification so the image pixels are larger (say 1.2um ). I could go to
100x and 512 horizontal image pixel so each image pixel is 2.3um?

I'm looking for slight changes in element concentration at grain boundaries
and precipitates and of course I want to produce the most meaningful data
possible. I'm running at conditions that suggest my electron interactive
volume is larger than my spatial resolution as to produce "continuous"
data.

Or am I just over thinking the process?

Any suggestion or comments would be welcome

Frank
Lincoln Electric Company
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From: microwink-at-gmail.com
Date: Thu, 20 Jan 2011 22:56:07 -0600
Subject: [Microscopy] Re: viaWWW: TEM diffraction 3nm precipitates

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Hello Dr. Zhou,

Our research group has also struggled with the challenge of obtaining
quality diffraction patterns from fine precipitates. The problem is
magnified (no pun intended) when the precipitates are very small, less
than 5nm in diameter, or when there is a high concentration of
precipitates with potentially different chemistry and crystal
structures (as is often the case with carbon replicas). The latter
problem prohibits the use of selected area diffraction techniques for
identifying individual precipitates as you will collect information
from many different precipitates with even the smallest SA aperture.
I'm only slightly surprised that you haven't been able to achieve good
results using nano beam diffraction. We've found that vanadium and
chromium-rich carbides are rapidly degraded by the electron beam. The
nano beam or convergent beam probe tends to damage the crystal
structure of the carbides as soon as the probe touches the
precipitate. We're not sure whether radiolysis or knock-on damage is
the main culprit since we've obtained less than ideal results at both
80kV and at 200kV. We have resorted to imaging the precipitates with
HRTEM and interpreting the FFTs to determine structure but we're still
making some attempts to use convergent beam probes to more rapidly
acquire diffraction patterns.

Good luck and keep us updated of your successes,
Chris
Drexel University - Dynamic Characterization Group

On Wed, Jan 19, 2011 at 3:47 PM, {z.zhou-at-lboro.ac.uk} wrote:
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} Email: z.zhou-at-lboro.ac.uk
} Name: Zhou
}
} Organization: Dept of Materials, Loughborough University, UK
}
} Title-Subject: [Filtered] TEM diffraction 3nm precipitates
}
} Message: Greetings listers,
}
} IŪm studying crystal structure of some
} carbides/nitrides precipitates in steel. The
} precipitates are 2-5nm size in a C replica TEM
} specimen, V-, Cr-, and Nb-rich by STEM/EDX.
}
} How can I get electron diffraction or crystal
} structure information of these fine precipitates?
}
} I tried on a TecnaiF20 TEM using nanoprobe spot
} 7, focused on a small particle, then diffraction,
} most of the time I got amorphous rings of C
} support, no obvious diffraction discs. STEM mode
} did indicate some C contamination on the session.
} Assuming minimal C contamination, is it possible
} that this method can give me some sort of
} convergence beam electron diffraction pattern,
} which may help me determine the crystal structure
} of the precipitates?
}
} IŪm also open to other methods.
}
} Your advice is highly appreciated.
}
} Zhou
}
} Dr Zhaoxia Zhou
} Dept of Materials
} Loughborough University
} Loughborough, UK
} LE11 3TU
}
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} ==============================Original Headers==============================
} 15, 24 -- From zaluzec-at-microscopy.com Wed Jan 19 14:33:42 2011
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} 15, 24 -- Subject: viaWWW: TEM diffraction 3nm precipitates
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}



--
Christopher Winkler
Dynamic Characterization Group
http://www.materials.drexel.edu/dcg/
Drexel University
267-496-0587


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From: kraftpiano-at-gmail.com
Date: Thu, 20 Jan 2011 23:08:26 -0600
Subject: [Microscopy] STM tip advice.

Contents Retrieved from Microscopy Listserver Archives
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I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).

I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.

The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.

Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.

Thanks,

Justin A. Kraft

==============================Original Headers==============================
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From: r-holdford-at-ti.com
Date: Fri, 21 Jan 2011 02:06:38 -0600
Subject: [Microscopy] Re: STM tip advice.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin: I used to make my own probe tips (for poking around
inside an IC) with tungsten wire and KOH. We had a set-up under
a microscope that applied a voltage (I think it was 5 volts and
an amp or so but don't remember exactly) between the wire and the
KOH (in a metal container) and you moved the wire in and out of
the KOH to form the tip. The surface tension of the KOH as the
wire moves in and out forms a nice pointy tip. It's best to have
something that controls the position and movement of the wire
mechanically unless you have very steady hands. Welding the wire
to the KOH container at 10-20X magnification will you make you jump!

On 1/20/11 11:08 PM, kraftpiano-at-gmail.com wrote:
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}
} I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).
}
} I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.
}
} The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.
}
} Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.
}
} Thanks,
}
} Justin A. Kraft
}
} ==============================Original Headers==============================
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214-567-0360
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From: benada-at-biomed.cas.cz
Date: Fri, 21 Jan 2011 03:07:44 -0600
Subject: [Microscopy] Re: Re: Microtome History - was "Ernst Ruska"

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
One of my friends told me that in the sixties, he and his colleagues were looking for
decommissioned broken black glass covers of gravestones. According to him, it was the best
material for glass knives.

Oldrich

On Thursday 20 of January 2011 16:54:51 you wrote:
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}
} I know at least 2 old microscopists who made
} glass knifes by breaking window glass - one of
} them here at CMU. He went to construction sites
} to get the broken windows.
}
} I'm running down the reference and (I hope) an
} image, but one of the early tries at
} ultramicrotomy was sticking razor blades on a
} centrifuge rotor, then mount the sections on the
} tub, close the lid and turn on the centrifuge.
} The sections where then picked up from inside the
} tub, having been flung willy-nilly around the
} inside.
} (The image is used in the microtomy lecture to
} convince the students thin sectioning could be a
} lot worse than they think it is.)
}
} Phil
}
} } Von: MuĢ Wolfgang
} } Gesendet: Donnerstag, 20. JĒnner 2011 11:46
} } An: 'joelsheffield-at-gmail.com'
} } Cc: 'microscopy-at-microscopy.com'
} } Betreff: Re: [Microscopy] Microtome History - was "Ernst Ruska"
} }
} } Joel wrote:
} } } Now, the urban legend.›
} }
} } I had heard that a group (unnamed) was having a
} } vigorous discussion about the best way to strop
} } a steel knife so as to get the best edge for
} } cutting thin sections.› How many strokes, in
} } which direction, which side of the blade, etc.›
} } During the discussion, a bottle of Coke was
} } knocked off the table and broke into many
} } pieces.›
} } The story goes that one, or another, of these
} } people took up one of the shards, and suggested
} } that it would make a good knife edge.
} } Verification anyone? {
} }
} } Joel
} }
} }
} } Good morning,
} } Dear Joel, dear all
} }
} } I can't tell or verify the above mentioned
} } "Coke-shard-story" but - indeed - I can add
} } information as to the "knocked off
} } table"-part....
} }
} } I had always heared (personal informations from
} } elder REICHERT-freaks and service people, from
} } lectures/lecturers in the 70ies and 80ies as
} } well as personally from H. Sitte himself) that
} } the group around H. SITTE at REICHERT (now
} } LEICA) in the late 1950ies/60ies was/were doing
} } such experiments with {glass plates} .... which
} } were thrown down to the floor... and one of them
} } (it was Sitte himself, as he told me) picked up
} } the shards evaluating the edges of "the most
} } promising one" for sectioning purposes (who of
} } that group had the "invention" to use glass as
} } a} crude { knife (element) I unfortunately don't
} } know.
} }
} } By the way, digging a little bit on this in
} } Google, I found one hint on a document in a
} } German data bank at
} } ==} http://finden.nationallizenzen.de/Author/Home?author=Sitte%2C%20H.›
} }
} } {Ein einfaches Ultramikrotom mit thermischem Vorschub} verĖffentlicht
} } ("A simple ultramicrotome with thermal advance" published)
} } in: Naturwissenschaften , ISSN 1432-1904, Vol. 42 (12. 1955), p. 367-367
} }
} } Note:› {Naturwissenschaften} is the former title
} } of››››› Biomedical and Life Sciences› (SPRINGER)
} } Another source:› http://www.freepatentsonline.com/3828641.html:
} } United States Patent US3828641:
} }
} } ==} Apparatus for adjusting the elevation of a
} } specimen in microtomes, particularly
} } ultramicrotomes
} } Inventor:› Hellmuth Sitte, HOMBURG (not Humburg
} } [as written in the patent document!]/Saar
} } Germany)
} } Assignee:› C.Reichert Optische Werke AG, Vienna, Austria)
} } Filed:› Nov. 10,1972
} }
} } Also see
} } http://books.google.at/books?id=JULNEc9rmUUC&pg=PA154&lpg=PA154&dq=Sitte+a
} } nd+ultramicrotome&source=bl&ots=jTsBEi9KOU&sig=EbNY_Cfv_isjcFo8MhMpJfl5Jb8
} } &hl=de&ei=aAs4Tf_AIp2ShAfA-tmMCg&sa=X&oi=book_result&ct=result&resnum=2&ve
} } d=0CCEQ6AEwAQ#v=onepage&q=Sitte%20and%20ultramicrotome&f=false (From the
} } book Michael J. Dykstra, Laura E.
} } Reuss - 2003› (see page "History: in the text
} } displayed
} } the Reichert OMU-1 (before 1964, perhaps see
} } document above in {Naturwissenschaften} 1955)
} } from Sitte described as having thermal advance,
} } Sitte was never happy with, since 1964: OMU-2
} } with thermal advance....
} }
} } This just for your pleasure....
} } (and I sure that searching for Sitte Hellmuth or
} } Sitte and microtome will find some results more
} } on that interesting and exciting "history" of
} } ultramicrotomy advances.....
} }
} } Best wishes and regards,
} } have a good and successful rest of the week and a beautiful weekend,
} }
} } Wolfgang MUSS (Ph.D.)
} } EM-Lab Gen.Hosp. SALK-LKH
} } SALZBURG
} } AUSTRIA
} }
} } } -----Ursprłngliche Nachricht-----
} } } Von: joelsheffield-at-gmail.com [mailto:joelsheffield-at-gmail.com]
} } } Gesendet: Donnerstag, 20. JĒnner 2011 06:51
} } } An: MuĢ Wolfgang
} } } Betreff: [Microscopy] Microtome History - was "Ernst Ruska"
} } }
} } } -----------------------------------------------------------------------
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} } }
} } } It is certainly clear that there are many interesting stories --some
} } } urban legends, and some insights into the minds of those that founded
} } } the discipline of thin section electron microscopy.
} } } During the mid '70's, I taught an EM course at Rutgers, Camden.
} } } In the basement, along with an RCA EMU-2 (!) was a thermal advance
} } } microtome that I was told was developed by Sjostrand.
} } } This thing consisted of a disk-like plate, about 4-5 inches in
} } } diameter, with an eccentrically mounted arm that stuck out of the
} } } plate.› The arm had a chuck for a tissue sample, and a heating coil.
} } } The plate itself was mounted in a frame and lubricated with oil --the
} } } plate acted like a large oil bearing.› The plate was linked to a motor
} } } on the wall by a long V belt (to minimize vibration), and the entire
} } } plate rotated, bringing the block past the knife on each rotation.› The
} } } sections that resulted tended to be arc shaped.› We got it working, but
} } } it was a terrifying thing to see.› I wonder if anyone in this group
} } } remembers this type of machine -- assuming that my description is
} } } comprehensible.
} } }
} } } The other microtome that I used, and really admire, was the Huxley
} } } microtome, originally sold by Cambridge, and ultimately marketed by
} } } LKB.› Although it used a mechanical advance, all of the movements of
} } } the cutting arm were made by bending sheets of spring steel, so that
} } } there were no surfaces that moved against each other.› Moreover, the
} } } cutting stroke was controlled by an oil-filled damper, to minimize
} } } chatter. -- a remarkable and stable machine.
} } }
} } } Now, the urban legend.
} } } I had heard that a group (unnamed) was having a vigorous discussion
} } } about the best way to strop a steel knife so as to get the best edge
} } } for cutting thin sections.› How many strokes, in which direction, which
} } } side of the blade, etc.› During the discussion, a bottle of Coke was
} } } knocked off the table and broke into many pieces.
} } } The story goes that one, or another, of these people took up one of the
} } } shards, and suggested that it would make a good knife edge.
} } } Verification anyone?
} } }
} } } Joel
} } }
} } } --
} } } Joel B. Sheffield, Ph.D.
} } } Biology Department, Temple University
} } } 1900 North 12th Street
} } } Philadelphia, PA 19122
} } } jbs-at-temple.edu
} } } (215) 204 8839, fax (215) 204 0486
} } } http://astro.temple.edu/~jbs


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From: protrain-at-emcourses.com
Date: Fri, 21 Jan 2011 05:45:19 -0600
Subject: [Microscopy] viaWWW: SEM imaging of wax

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

All I have been watching the advice on this problem and whilst I agree with
all that has been said may I offer a little more?

I have had experience with materials that forced us to use cryo within an
investigation, then we (the client and I) purchased an early FEGSEM. As you
may guess the cryo kit would not fit onto the FEG! Forced to take another
route we found that being very careful in the FEG we could work on the very
sensitive material without any apparent problems.

The settings we used were those typical for sensitive materials:-

1. Coat the specimen with a minimal current and an extended working
distance e.g. normal would be ~5cm we used 7cm from memory(?)
2. Use a low emission current.
3. Use a spot size/probe current near the lowest limit of the
instrument.
4. Use the out of lens detector for minimal charge (~15mm WD)

I hope that this may help?

Steve

Steve Chapman FRMS
Senior Consultant Protrain
For consultancy and training in electron microscopy world wide
Tel +44 (0)1280 816512 Fax +44 (0)1280 814007 Cell +44 (0)7711 606967
www.emcourses.com



-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: 20 January 2011 17:58
To: protrain-at-emcourses.com

Dear All,
I had the same problem visualizing bee wax structures and not owning an ESEM
;-)

Solution: I put the structure on a large specimen mount, putting 2 bands of
conductive adhesive tape left and right on top and put
it in my normal Pt-sputtercoater.
Concerning the heat during sputtering, I took off the sputter-head and added
a second glass vessel (I took it from my
carbon-coater) on top of the first.
Did ca. 10-15 sputtering-runs with minimal current (10mA) and finally put it
in the scope at 5-6kV.

See images at
http://www.electronmicroscopy.info/wax

It`s a bit of a crude solution but it works.
Concerning the thickness of your wax layers you may also try the same thing
with an high-vac chromecoater...


Best wishes,
Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 20.01.11 16:00, schrieb nizets2-at-yahoo.com:
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} Just a question disguised in an answer: wouldn't it be the right
application to
} make a replica?
}
} Stephane
}
}
} ----- Original Message ----
} X-from: "anthonyribaudo3-at-gmail.com" {anthonyribaudo3-at-gmail.com}
} To: nizets2-at-yahoo.com
} Sent: Wed, January 19, 2011 9:39:49 PM
} Subject: [Microscopy] viaWWW: SEM imaging of wax
}
}
}
}
}
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} Email: anthonyribaudo3-at-gmail.com
} Name: Anthony Ribaudo
}
} Organization: TRI Princeton
}
} Title-Subject: [Filtered] SEM imaging of wax
}
} Message: Can anyone suggest guidelines for imaging a paraffin wax
} coating on a metal or polymeric substrate via FESEM. The plan is to
} detect the wax layer that is thought to be several hundred nanometers
} thick? Are there any waxes that would be more stable to image under
} the electron beam than paraffin wax?
}
} Anthony Ribaudo
} TRI Princeton
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} Login Host: 108.5.140.150
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From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Fri, 21 Jan 2011 09:29:38 -0600
Subject: [Microscopy] Re: Environmental SEM for Biological Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

I'm not a biologist (most materials science) nor experimented in ESEM
(only high vac SEM), but I've on my shelve a thermoelectric module
(Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.

Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf,
where you can find a great variety of such devices. They give a Dtį in
the 70įC range, and are avaible in several dimensions and power. From
10x10 to 62x62 are proposed, but rectanglaire or round shapes too.
Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the
hot side is low. There is probably room enough to put a heat sink on the
stage (copper block), but possibly one would need a water cooled one,
what is probably not necessary on the device provided by FEI.
If you have the possibilty to build something yourselve, it could be
much cheaper. Of coarse without performences waranty !

Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matťriaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote:
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} Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter.
}
} You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe.
}
} Does anyone know if this means the sample can be any size one likes?
}
} Dave
}
} -----Original Message-----
} X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu]
} Sent: 19 January 2011 22:53
} To: David Patton
} Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work
}
}
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} Email: rfoley-at-uab.edu
} Name: Robin Foley
}
} Organization: UAB
}
} Title-Subject: [Filtered] Environmental SEM for Biological Work
}
} Message: Is there heavy use of ESEM for biological SEM? What is
} commonly done on biological ESEM samples? We're looking at
} purchasing an environmental SEM and the sales rep. tells us we will
} need a Peltier stage to cool the sample to look at wet samples.
} Unfortunately, the maximum sample size for the Peltier stage is 3 mm
} across. This seems tiny to me for an SEM. Are there many biological
} ESEM applications that don't require the sample to be wet?
}
} Thanks,
}
} Robin Foley (who spends most of her SEM time looking at metals,
} ceramics and polymers!)
}
}
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From: DusevichV-at-umkc.edu
Date: Fri, 21 Jan 2011 10:44:52 -0600
Subject: [Microscopy] RE: EDS line scans and time on my hands

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Frank,

Have you run spot analysis (boundary and middle of a grain)
to confirm you can detect difference? From my experience with
iron specimens, it is a pretty tough task to see boundary segregations
even with WDS (at least when it is not a second phase, like carbides).
X-from spot analysis you can estimate time needed for acquisition.

I do not know what element you are going to analyze, but if it is
phosphorous, 20 kV is a way too high. For P you can use 4-5 kV and
have a nice small zone of interaction.

Good luck

Vladimir

Vladimir M. Dusevich, Ph.D.
Electron Microscope Lab Manager
371 School of Dentistry
650 E. 25th Street
Kansas City, MO 64108-2784

} from: Frank_Karl-at-lincolnelectric.com
} Date: 01/19/2011 05:45 AM
}
} I've been thinking about EDS line scans and I'm attempting to maximize
} The data I collect.
}
} I run at 20 KV on iron samples so my electron interaction range is
} between
} 1.5 to 2.3um (Berthe or K-O range calculations). According to data
} provided by EDAX at 1024 horizontal pixels each pixel is around 0.8 um
} at
} 700x. So right away I see if each image pixel represents a data point,
} I'm
} sampling about 2 pixel volumes per data point. Looking for a sudden
} and
} sharp change in element concentration seem difficult, but wait there's
} more. Let us introduce another plot complication.
}
} My customers don't want to scan the entire image, tooo much data!
}
} So I scan a smaller section of image described above and instruct the
} computer to take 512 data points along a line that might only be 400
} (visual estimate from screen) image pixels long.
}
} So... Should I reduce the image resolution so that each image pixel
} will be
} one data point and scan the entire image? Would it be better to lower
} the
} magnification so the image pixels are larger (say 1.2um ). I could go
} to
} 100x and 512 horizontal image pixel so each image pixel is 2.3um?
}
} I'm looking for slight changes in element concentration at grain
} boundaries
} and precipitates and of course I want to produce the most meaningful
} data
} possible. I'm running at conditions that suggest my electron
} interactive
} volume is larger than my spatial resolution as to produce "continuous"
} data.
}
} Or am I just over thinking the process?
}
} Any suggestion or comments would be welcome
}
} Frank
} Lincoln Electric Company
} --
} *************************************************************
} Note:
} The information contained in this message may be
} privileged and confidential and protected from disclosure. If
} the reader of this message is not the intended recipient, or
} an employee or agent responsible for delivering this message
} to the intended recipient, you are hereby notified that any
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From: cdh1-at-cdc.gov
Date: Fri, 21 Jan 2011 11:51:58 -0600
Subject: [Microscopy] Protocol for Norovirus TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

I replied to Debby Sherman offline. In general stool specimen
suspensions can be fixed in 2.5-5% paraformaldehyde prior to shipping.
The specimens are then rendered noninfectious and can be maintained for
an indefinite period at 4 degrees C. Multiple freezing and thawing, on
occasion, can cause deleterious effects to viruses sufficient to render
them difficult to identify by diagnostic negative stain electron
microscopy.

Best regards,

Charles

Charles Humphrey
US Centers for Disease Control and Prevention
Mailstop G32
1600 Clifton Rd.
Atlanta, GA 30333


-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Wednesday, January 19, 2011 4:46 PM
To: Humphrey, Charles (CDC/OID/NCEZID)

Hi all,

We will be imaging a fairly large number of norovirus samples using
negative
staining. This is just for sample screening to document the presence or
absence of the virus.

The samples could be sent in fresh but I thought it might be better to
fix
them prior to shipping so that they are not a health hazard. That might
also
allow us to store them unfrozen for an extended period of time (months)
with
reduced chance of deterioration or contamination.

Does anyone have a validated protocol that they are willing to share? I
used to know someone at the CDC who did TEM but no longer have that
contact.

Thanks,
Debby
--
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy/



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From: cpawlowicz-at-ubmtechinsights.com
Date: Fri, 21 Jan 2011 12:09:20 -0600
Subject: [Microscopy] RE: STM tip advice.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Justin,

What is it about your tips that don't function? Are you sure it's not the sample or settings on the tool?

I spent some time last year doing STM with a DI3100 (/Veeco/Bruker) and made many many tips using the side cutter + pull method using Pt/Ir wire. I got some great images from HOPG samples to prove to myself that it was working properly.

The HOPG is highly recommended to get familiar with things (I got my sample from SPI, but Pella also has them). It will guarantee that your sample is not the problem, and allow low-mag imaging (over steps in layers) to very high mag imaging (lattice) to confirm that you have everything working properly.

What worked well for me was I would cut a tip slightly longer than I needed, then held one end with pliers while diagonal cutting/pulling away just the tip at the other end. The direction of cut started nearer the fixed end/pliers and ended up towards the free end. The part that was cut off (stuck to the cutters, flew across the table etc) was junk, the part left gripped in the pliers had a cut/stretched shape but was torn and jagged at the very end (under a microscope).

If you think about the way the sidecutters cut through the metal and plan on having the very end 'torn' you should be able to reliably get excellent tips. I taught the method to a couple of other people and they quickly managed to make similar tips without much trouble.

Chris Pawlowicz, B.Eng
Manager, Lab Development
UBM TechInsights
3000 Solandt Rd Ottawa ON Canada K2K 2X2
T: +1 613 576 0150
F: +1 613 599 6501
E: cpawlowicz-at-ubmtechinsights.com


---------------------------------------------------------------------------

I know that I usually talk about SEM applications, but recently I have been given the task of setting up and playing with a scanning tunneling microscope. We have a small length of platinum/iridium wire, and the instructions tell us to take a pair of cutters and gently squeeze and pull the wire to make an atomic scale tip. So far, we have been unsuccessful at producing a tip that functions properly in the machine (It's a Easyscan Nanosurf).

I found some documentation online about etching tungsten tips in KOH, so I sacrificed an SEM filament and tried it- at first it looked good, but the tip still ended up etched entirely flat at the surface of the KOH solution.

The purpose of my playing with this is to try to develop a set of instructions that anyone (Physics undergrads- most of whom are theorists and think less of us experimentalists) can use to get this up and running for one of our lab courses.

Does anyone have any suggestions? Since this is a lab course, there is no budget to be spending $200 per tip on tips that will just end up smashed into the surface of a specimen anyway.

Thanks,

Justin A. Kraft


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From: FMonson-at-wcupa.edu
Date: Fri, 21 Jan 2011 13:56:00 -0600
Subject: [Microscopy] Re: Environmental SEM for Biological Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Something is always going on in VP and/or Environmental SEM: http://www.microscopy.org.au/ACMM20/VPSEMWorkshopOutline.pdf
And, even a book: http://www.amazon.com/Principles-Practice-Variable-Pressure-Environmental/dp/0470065400

I have attended VP workshops at M&M meetings and I am reading the book.

Know it before you buy it. Our Quanta 400 (100mm stage) Mk1 has a water-cooled thermoelectric cold stage that will permit a specimen no larger than about 7mm. NOTE: PV=NRT is the key when one uses water vapor to pressurize the environment of an hydrated specimen.

Brendan Griffin has, I believe, done his original work on a XL-30, so he might have some suggestions as to what you might do in your deliberations.

My offerings of advice are as follows:
1. W or FEG - depends on requirements for resolution and data acquisition and analysis, because FEG is more expensive to maintain but absolutely necessary for high res imaging work alone.
2. Pressure ranges in VP SEM range from 1 Torr max to 20+ Torr max depending on platform, and therefore, the choice of platform will be based on range of expected uses to which the tool will be put and which gases one may wish to use.
3. Examples of uses we make of VP
a. With our fully automated Oxford INCA EDS w/Feature/GSR, I have surveyed a 95 x 95 x 10mm slab of rock for grayscale thresholded ROI's or OOI's (2 sec acquisitions / object identified in a field) for OOI's so that the geologist could determine the area of the rock from which s/he would retrieve a thin section for further survey and probable selection for electron probe analysis (for dating).
b. uncoated microfossils for imaging and retrieval.
c. Backscatter image montages of large specimens (e.g. up to size in 1)
d. There were rumors that at least on user imaged a specimen using H2SO4 vapor.
e. Montage maps of large inclusions.
4. Before determining a choice, carefully create a list of expected and projected applications.
a. The list defines the tool, if the list is very carefully assembled.
5. ALWAYS make know the total cost of ownership, including personnel, for a decade, and include in purchase as much annual service as possible.
6. Carefully spec the operating systems on the controlling computers with a sure knowledge of what will happen when the OS ceases to be supported, and user interface program will cease being supported by vendor. With our ESEM, the UI lost support before the OS (Win 2k Pro) did.
a. My learned approach would be to assure management that THIS part of future support is clearly understood by both the supplier and the purchaser, with clear guarantees by the vendor in the purchase contract. That is, when/if there is a clear glitch in the Vendor's UI, there is a clear guarantee of support for a declared period of time.
b. Recognize that each of your instruments may be the only one in the world to manifest a specific problem, and that the vendor's incentive is driven by numbers.
c. Require a list of 3rd-party parts of the system you are purchasing. You will want to know how fare in the future your legacy bottlenecks might be.
d. Recognize that in my world, at least, a control PC may cost a few to tens of thousands of dollars when replaced by the vendor when there is no contract. Ask what a stage replacement is at the moment, a control PC, a User Interface upgrade, and an estimate of what an upgrade of the disk operating system of the PC and the tool might be in 3-7 years (no vendor wants to give such an estimate, but remember that you have what they want.
e. Recognize that you are likely to have any downtime reduced by turning service over to a 3rd party for an instrument under 10 years of age. Our TEM, after 8 years in service is quite similar in all respects to the model that is currently being marketed by FEI. FEI service on everything, and I strongly recommend it.
7. Make a set of the support documentation and parts lists a line item on your specification, and that if part numbers are changed in the future, you will be able to acquire them at no cost (for as long as you own the instrument).
7. The other matter is less obvious. Carefully spec your requirements for HV regulation and stability, in the context of your requirements for stability during an analysis as well as long-term reproducibility. This is difficult, but the HV system quite sophisticated and is repaired by total replacement rather than component replacement.

Finally, when our web site is back on line sometime next week, there are several papers related to VP SEM on the "Links" page that might be of interest to you and your prospective users.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl


-----Original Message-----
X-from: jacques.faerber-at-ipcms.u-strasbg.fr [mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
Sent: Friday, January 21, 2011 10:41 AM
To: Monson, Frederick

Hi

I'm not a biologist (most materials science) nor experimented in ESEM
(only high vac SEM), but I've on my shelve a thermoelectric module
(Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.

Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf,
where you can find a great variety of such devices. They give a Dtį in
the 70įC range, and are avaible in several dimensions and power. From
10x10 to 62x62 are proposed, but rectanglaire or round shapes too.
Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the
hot side is low. There is probably room enough to put a heat sink on the
stage (copper block), but possibly one would need a water cooled one,
what is probably not necessary on the device provided by FEI.
If you have the possibilty to build something yourselve, it could be
much cheaper. Of coarse without performences waranty !

Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matťriaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote:
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} Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs about 10mm in diameter.
}
} You can use our ESEM without cooling for eg materials samples but we you want liquid water for biological samples we cool to 5 degrees and 6.5 Torr. I have heard that the newer Quanta ESEMs can have a chamber pressure up to 20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed without cooling, I believe.
}
} Does anyone know if this means the sample can be any size one likes?
}
} Dave
}
} -----Original Message-----
} X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu]
} Sent: 19 January 2011 22:53
} To: David Patton
} Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work
}
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} Email: rfoley-at-uab.edu
} Name: Robin Foley
}
} Organization: UAB
}
} Title-Subject: [Filtered] Environmental SEM for Biological Work
}
} Message: Is there heavy use of ESEM for biological SEM? What is
} commonly done on biological ESEM samples? We're looking at
} purchasing an environmental SEM and the sales rep. tells us we will
} need a Peltier stage to cool the sample to look at wet samples.
} Unfortunately, the maximum sample size for the Peltier stage is 3 mm
} across. This seems tiny to me for an SEM. Are there many biological
} ESEM applications that don't require the sample to be wet?
}
} Thanks,
}
} Robin Foley (who spends most of her SEM time looking at metals,
} ceramics and polymers!)
}
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From: kenconverse-at-qualityimages.biz
Date: Fri, 21 Jan 2011 15:16:34 -0600
Subject: [Microscopy] Re: Environmental SEM for Biological Work

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Fred,
Could you elaborate on item 6e? I'm a little confused.

I like some of your ideas to illuminate the probable life span of a system.
Back when dinosaurs roamed the Earth, most people didn't pay much attention
to life span because it was (usually correctly) assumed to be long.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: FMonson-at-wcupa.edu [mailto:FMonson-at-wcupa.edu]
Sent: Friday, January 21, 2011 2:58 PM
To: kenconverse-at-qualityimages.biz

Something is always going on in VP and/or Environmental SEM:
http://www.microscopy.org.au/ACMM20/VPSEMWorkshopOutline.pdf
And, even a book:
http://www.amazon.com/Principles-Practice-Variable-Pressure-Environmental/dp
/0470065400

I have attended VP workshops at M&M meetings and I am reading the book.

Know it before you buy it. Our Quanta 400 (100mm stage) Mk1 has a
water-cooled thermoelectric cold stage that will permit a specimen no larger
than about 7mm. NOTE: PV=NRT is the key when one uses water vapor to
pressurize the environment of an hydrated specimen.

Brendan Griffin has, I believe, done his original work on a XL-30, so he
might have some suggestions as to what you might do in your deliberations.

My offerings of advice are as follows:
1. W or FEG - depends on requirements for resolution and data
acquisition and analysis, because FEG is more expensive to maintain but
absolutely necessary for high res imaging work alone.
2. Pressure ranges in VP SEM range from 1 Torr max to 20+ Torr
max depending on platform, and therefore, the choice of platform will be
based on range of expected uses to which the tool will be put and which
gases one may wish to use.
3. Examples of uses we make of VP
a. With our fully automated Oxford INCA EDS
w/Feature/GSR, I have surveyed a 95 x 95 x 10mm slab of rock for grayscale
thresholded ROI's or OOI's (2 sec acquisitions / object identified in a
field) for OOI's so that the geologist could determine the area of the rock
from which s/he would retrieve a thin section for further survey and
probable selection for electron probe analysis (for dating).
b. uncoated microfossils for imaging and retrieval.
c. Backscatter image montages of large specimens (e.g.
up to size in 1)
d. There were rumors that at least on user imaged a
specimen using H2SO4 vapor.
e. Montage maps of large inclusions.
4. Before determining a choice, carefully create a list of
expected and projected applications.
a. The list defines the tool, if the list is very
carefully assembled.
5. ALWAYS make know the total cost of ownership, including
personnel, for a decade, and include in purchase as much annual service as
possible.
6. Carefully spec the operating systems on the controlling
computers with a sure knowledge of what will happen when the OS ceases to be
supported, and user interface program will cease being supported by vendor.
With our ESEM, the UI lost support before the OS (Win 2k Pro) did.
a. My learned approach would be to assure management
that THIS part of future support is clearly understood by both the supplier
and the purchaser, with clear guarantees by the vendor in the purchase
contract. That is, when/if there is a clear glitch in the Vendor's UI,
there is a clear guarantee of support for a declared period of time.
b. Recognize that each of your instruments may be the
only one in the world to manifest a specific problem, and that the vendor's
incentive is driven by numbers.
c. Require a list of 3rd-party parts of the system you
are purchasing. You will want to know how fare in the future your legacy
bottlenecks might be.
d. Recognize that in my world, at least, a control PC
may cost a few to tens of thousands of dollars when replaced by the vendor
when there is no contract. Ask what a stage replacement is at the moment, a
control PC, a User Interface upgrade, and an estimate of what an upgrade of
the disk operating system of the PC and the tool might be in 3-7 years (no
vendor wants to give such an estimate, but remember that you have what they
want.
e. Recognize that you are likely to have any downtime
reduced by turning service over to a 3rd party for an instrument under 10
years of age. Our TEM, after 8 years in service is quite similar in all
respects to the model that is currently being marketed by FEI. FEI service
on everything, and I strongly recommend it.
7. Make a set of the support documentation and parts lists a
line item on your specification, and that if part numbers are changed in the
future, you will be able to acquire them at no cost (for as long as you own
the instrument).
7. The other matter is less obvious. Carefully spec your
requirements for HV regulation and stability, in the context of your
requirements for stability during an analysis as well as long-term
reproducibility. This is difficult, but the HV system quite sophisticated
and is repaired by total replacement rather than component replacement.

Finally, when our web site is back on line sometime next week, there are
several papers related to VP SEM on the "Links" page that might be of
interest to you and your prospective users.

Hope this helps,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl


-----Original Message-----
X-from: jacques.faerber-at-ipcms.u-strasbg.fr
[mailto:jacques.faerber-at-ipcms.u-strasbg.fr]
Sent: Friday, January 21, 2011 10:41 AM
To: Monson, Frederick

Hi

I'm not a biologist (most materials science) nor experimented in ESEM
(only high vac SEM), but I've on my shelve a thermoelectric module
(Pelletier/Seebeck device) which is 1.5x4 cm in dimensions.

Have a look at http://www.knap.at/datenblaetter/pel/pel_sup_kat-tem-e.pdf,
where you can find a great variety of such devices. They give a Dtį in
the 70įC range, and are avaible in several dimensions and power. From
10x10 to 62x62 are proposed, but rectanglaire or round shapes too.
Of coarse, as an ESEM is under (bad) vacuum, thermal dissipation of the
hot side is low. There is probably room enough to put a heat sink on the
stage (copper block), but possibly one would need a water cooled one,
what is probably not necessary on the device provided by FEI.
If you have the possibilty to build something yourselve, it could be
much cheaper. Of coarse without performences waranty !

Hope it helps

J. Faerber
IPCMS-GSI
(Institut de Physique et Chimie des Matťriaux de Strasbourg
Groupe Surface et Interfaces)
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail Jacques.Faerber-at-ipcms.u-strasbg.fr


On 20/01/2011 13:02, David.Patton-at-uwe.ac.uk wrote:
}
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} Our Philips (now FEI) XL30 ESEM has a Peltier stage which takes stubs
about 10mm in diameter.
}
} You can use our ESEM without cooling for eg materials samples but we you
want liquid water for biological samples we cool to 5 degrees and 6.5 Torr.
I have heard that the newer Quanta ESEMs can have a chamber pressure up to
20 Torr (cf 10 Torr for the XL30 ESEM). This allows water to be observed
without cooling, I believe.
}
} Does anyone know if this means the sample can be any size one likes?
}
} Dave
}
} -----Original Message-----
} X-from: rfoley-at-uab.edu [mailto:rfoley-at-uab.edu]
} Sent: 19 January 2011 22:53
} To: David Patton
} Subject: [Microscopy] viaWWW: Environmental SEM for Biological Work
}
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} Email: rfoley-at-uab.edu
} Name: Robin Foley
}
} Organization: UAB
}
} Title-Subject: [Filtered] Environmental SEM for Biological Work
}
} Message: Is there heavy use of ESEM for biological SEM? What is
} commonly done on biological ESEM samples? We're looking at
} purchasing an environmental SEM and the sales rep. tells us we will
} need a Peltier stage to cool the sample to look at wet samples.
} Unfortunately, the maximum sample size for the Peltier stage is 3 mm
} across. This seems tiny to me for an SEM. Are there many biological
} ESEM applications that don't require the sample to be wet?
}
} Thanks,
}
} Robin Foley (who spends most of her SEM time looking at metals,
} ceramics and polymers!)
}
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From: donc-at-asmicro.com
Date: Sat, 22 Jan 2011 15:49:25 -0600
Subject: [Microscopy] Re: SEM stardard grid - high resolution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jun asked for a calibration grid with pitch near 0.1 um.
My company makes and sells two relevant products:
-a 70-nm pitch 1-dimensional pattern (lines and spaces) and
-a 144-nm pitch square grid.
These are available as ordinary calibration specimens or as standards
traceable to the international meter. The traceable standards come with a
test report that shows both the pitch value and its uncertainty.
Please find further details at:
http://www.asmicro.com/Supplies/utc.htm - traceable calibration specimens.

http://www.asmicro.com/Supplies/150-2D-Calibration-Specimen.htm

http://www.asmicro.com/Supplies/70-1D.htm



regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.] 2009-2010 is our 20th year in business!
=============================================

----- Original Message -----
From: junhe1970-at-gmail.com
To: donc-at-asmicro.com
Sent: Tuesday, December 21, 2010 8:05 AM
Subject: [a] [Microscopy] viaWWW: SEM stardard grid





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Email: junhe1970-at-gmail.com
Name: jun

Title-Subject: [Filtered] SEM stardard grid

Message: We frequently uses SEM ( Hitachi 4700, FE) to measure some
wafer cross-section features.
The measurement result is sometimes off the mark .
I wonder if some one here could recommend some fine grid (0.1
um)standard
Jun



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21, 35 -- From donc-at-asmicro.com Sat Jan 22 15:49:25 2011
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21, 35 -- References: {201012211305.oBLD5eww027041-at-ns.microscopy.com}
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From: vakimler-at-med.wayne.edu
Date: Mon, 24 Jan 2011 11:11:03 -0600
Subject: [Microscopy] viaWWW: Post-embedding/sectioning FluoroNanogold Labeling for TEM

Contents Retrieved from Microscopy Listserver Archives
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Email: vakimler-at-med.wayne.edu
Name: Vickie Kimler

Organization: Wayne State Univ. School of Medicine

Title-Subject: [Filtered] Post-embedding/sectioning FluoroNanogold
Labeling for TEM

Message: Hello,
Some of our antigens are difficult to label in mammalian fat cells
and muscles, such as particular lipid droplet proteins. I have tried
several methods of antigen unmasking and probe maintenance in
post-sectioning fluoronanogold labeling, including absence of uranyl
acetate in the LR-White embedding medium, reduction of osmium
post-fixation concentration to reduce etching propensity of
silver-enhanced gold probes, antigen unmasking with oxidizing (sodium
metaperiodate) and reducing (sodium borohydride) agents and Tris base
pH 10.
I have reduced my glutaraldehyde concentration to 0.10% and
maintained paraformaldehyde at 2%.

Pre-embedding fluoronanogold labeling appears to work with the
absence of glutaraldehyde in the fixative, however the ultrastructure
is quite compromised, as one would expect by TEM.

I have been thinking now of using LR-Gold, until I hear from some
experts on any other suggestions. Thank you very much!
Vickie



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10, 22 -- Subject: viaWWW: Post-embedding/sectioning FluoroNanogold Labeling for TEM
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From: j.knowles-at-ucl.ac.uk
Date: Mon, 24 Jan 2011 11:11:55 -0600
Subject: [Microscopy] viaWWW: Electronics side of an EDX system

Contents Retrieved from Microscopy Listserver Archives
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Email: j.knowles-at-ucl.ac.uk
Name: Jonathan Knowles

Organization: UCL

Title-Subject: [Filtered] Electronics side of an EDX system

Message: Hi

We have inherited an EDX system which seems to work OK (sorry I don't
have details to hand but wanted to ask this before I forgot!). The
system works OK, but the electronics side is really ancient (It has a
microVAX type drive with about 20Mb storage to run the OS and
software) and am concerned that it might die some time. I wondered if
anyone had ever built some more up to date hardware/interface? The
connections for the EDX consist of a BNC for the HT and then a 9 pin
connector presumably for data.

Thanks

Jonathan

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From: xyang-at-SMU.CA
Date: Mon, 24 Jan 2011 13:12:57 -0600
Subject: [Microscopy] RE: middle mouse button problem in LEO SEM system

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Dear Listers,

I am running a LEO1450VP SEM and encounter a problem related to the use of
the mouse.† The middle button of a 3-key mouse is used extremely in LEO32
software and suddenly the software disable the use of the middle button.

I am able to use both left and right button, except the middle one.† I also
tried to edit the toolbar to set the middle buttonís function. However, both
the ďOKĒ and ďApplyĒ buttons were deactivated so the settings I made were
useless.

If anyone has similar experiences, please kindly contact me.† Thank you very
much.

Sean
EMC -at- Saint Maryís University
Halifax, NS
xyang-at-smu.ca




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From: reynolds-at-vt.edu
Date: Mon, 24 Jan 2011 14:02:35 -0600
Subject: [Microscopy] viaWWW: Fwd: EDS line scans and time on my hands

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Email: reynolds-at-vt.edu
Name: Bill Reynolds

Organization: Virginia Tech

Title-Subject: [Filtered] Fwd: EDS line scans and time on my hands

Message: This comment does not directly address Frank Karl's original
question about pixel size and measurement spacing, but it relates to
the problem of detecting small changes in boundary concentrations.

Measuring grain boundary concentrations from EDS line scans is
problematic because the electron beam geometry is always convoluted
with the solute distribution in the sample. To determine, say, the
maximum solute concentration at a grain boundary, you have to know
the extent of beam broadening in the sample AND have an accurate
model for
how the solute is distributed across the boundary. If you don't
already know where the solute is, you have to make an educated guess.
For example, you might assume the solute is confined to a boundary
region 1 nm thick so you can back-out a boundary concentration from
the measured EDS profile, and the probe size with broadening. That
involves a lot of assumptions and uncertainties.

A clever alternate strategy is to make an integrated measurement
across a boundary to determine the total excess solute associated
with the boundary (the excess solute is the amount of solute per unit
area of grain boundary). This quantity is calculated from the
difference between the amount of solute found in a sample volume that
includes the boundary and the amount of solute found in an equivalent
volume without the boundary (but located nearby).

The excess solute is not a concentration, and it tells you nothing
about the solute distribution at a boundary, but it has some distinct
advantages when trying to quantify solute enrichment or depletion at
a boundary: it can be measured without knowing much about the probe
size, beam broadening, or boundary structure, and you do not need to
assume anything about the solute profile. Because the method employs
a scanned box that straddles a boundary, it has better counting
statistics than single line scans, and that makes it pretty good at
detecting small solute enrichments.

The technique was developed with STEM in mind, but I don't see any
reason why it cannot be used for EDS measurements in an SEM. Here
are some example investigations that used the method:
J.A.S. Ikeda, Y.-M. Chiang, and C.G. Madras: Ceram. Trans., 1991,
vol. 24, pp. 341-348
A. J. Garratt-Reed: Proceedings of the 50th Annual Meeting, EMSA,
1992, p. 1206-1207
H.A. Fletcher, et al.: Scripta Mater., 2001, vol. 45 (5), pp. 561-567
E. S. Humphreys, et al. Metall. Mat. Trans A. vol 35A (4), 2004, pp 1223-1235.

Regards,
Bill Reynolds
Virginia Tech


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From: sbarlow-at-sciences.sdsu.edu
Date: Mon, 24 Jan 2011 14:03:02 -0600
Subject: [Microscopy] viaWWW: Uninterruptible power supply

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Email: sbarlow-at-sciences.sdsu.edu
Name: Steve Barlow

Organization: EM Facility/San Diego State U Biology

Title-Subject: [Filtered] Uninterruptible power supply

Message: I am looking for a ups system for my Quanta 450 FESEM. If
anyone has any experiences with various models, I would appreciate
hearing about it.

The needed characteristics are:
Rating= 8 kVA/6.4 kW
Input voltage= 172-285 Vac
Input frequency= 40-70 Hz
Output voltage= 230 Vac
Output frequency= 50/60 Hz

Thanks in advance. Vendors may contact me directly offline

Steve

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From: gabriel.lapointe2-at-gmail.com
Date: Mon, 24 Jan 2011 14:42:48 -0600
Subject: [Microscopy] Re: viaWWW: Uninterruptible power supply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A good source of knowledge for these thing is your IT department.
Servers, especially those used in high power computing, tends to consume
a lot of current and tend to fail badly when the power is interrupted.

cheers,
logo



Gabriel Lapointe, M.Sc.
gabriel.lapointe2-at-gmail.com
http://gabriellapointe.ca




logo





On Mon, 2011-01-24 at 14:11 -0600, sbarlow-at-sciences.sdsu.edu wrote:

} Email: sbarlow-at-sciences.sdsu.edu
} Name: Steve Barlow
}
} Organization: EM Facility/San Diego State U Biology
}
} Title-Subject: [Filtered] Uninterruptible power supply
}
} Message: I am looking for a ups system for my Quanta 450 FESEM. If
} anyone has any experiences with various models, I would appreciate
} hearing about it.
}
} The needed characteristics are:
} Rating= 8 kVA/6.4 kW
} Input voltage= 172-285 Vac
} Input frequency= 40-70 Hz
} Output voltage= 230 Vac
} Output frequency= 50/60 Hz
}
} Thanks in advance. Vendors may contact me directly offline
}
} Steve
}
} Login Host: 146.244.234.42


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From: gul417-at-mail.usask.ca
Date: Mon, 24 Jan 2011 18:12:50 -0600
Subject: [Microscopy] viaWWW: Edwards S150B sputter coater part: insulator/cable

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Email: gul417-at-mail.usask.ca
Name: Guosheng Liu

Organization: University of Saskatchewan

Title-Subject: [Filtered] Edwards S150B sputter coater part: insulator/cable

Message: Hello,

The insulator cable in our aged Edwards S150B sputter coater broke
(so no HT) a few days ago. Does anyone have a clue on where can I
order this part (or a compatible part from other makes) or get a
service (I'm in Canada)?

Thanks in advance.

Guosheng

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From: ian.dobbie-at-bioch.ox.ac.uk
Date: Tue, 25 Jan 2011 07:26:32 -0600
Subject: [Microscopy] Recruiting correlative light/EM specialist, assistant facility manager and image analysis specialist.

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Hi,

We are recruiting a number of posts here in Oxford (UK), several of which
might be of particular interest to readers here. If you know anyone who
might be interested in these posts please do forward them this info. The
closing dates are all Feb 3rd.

Micron Oxford is funded by a major grant from the Wellcome Trust to the
Department of Biochemistry and the Sir William Dunn School of Pathology
in association with the Wellcome Trust Centre for Human Genetics and The
Oxford Center for Integrative Systems Biology. Its aim is to establish
state of the art light and electron microscopy facilities located in the
heart of the Oxford University research community with an emphasis on
technology development.

We are currently trying to recruit an Assistant Facility Manager, a
Correlative Light/Electron Microscopy Specialist and an Image Analysis
Specialist. Full details can be found on our website
http://www.micronoxford.com/

Additionally we are looking to appoint a Group Leader with expertise in
the development or application of advanced imaging techniques or the
modulation of protein function with a background in physics, chemistry
or biology. We are also looking to appoint at senior post-doctoral level
a system administrator specialising in the area of hierarchical data
storage development/management. Full particulars of each post can be
found at the Micron website (http://www.micronoxford.com/).

Thanks,

Ian
--
Ian Dobbie
Micron Imaging Facility Manager,
Biochemistry,
University of Oxford,
South Parks Road,
Oxford
OX1 3QU
Tel: +44 (0)1865 613323
Email: ian.dobbie-at-bioch.ox.ac.uk

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From: chaueter-at-bcm.edu
Date: Tue, 25 Jan 2011 11:20:03 -0600
Subject: [Microscopy] Reply on Post-embedding/sectioning FluoroNanogold labeling for TEM

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Email: vakimler-at-med.wayne.edu
Name: Vickie Kimler

Organization: Wayne State Univ. School of Medicine

Title-Subject: [Filtered] Post-embedding/sectioning FluoroNanogold Labeling for TEM

Message: Hello,
Some of our antigens are difficult to label in mammalian fat cells and muscles, such as particular lipid droplet proteins.
I have tried several methods of antigen unmasking and probe maintenance in post-sectioning fluoronanogold labeling,
including absence of uranyl acetate in the LR-White embedding medium, reduction of osmium post-fixation concentration to
reduce etching propensity of silver-enhanced gold probes, antigen unmasking with oxidizing (sodium
metaperiodate) and reducing (sodium borohydride) agents and Tris base pH 10.
I have reduced my glutaraldehyde concentration to 0.10% and maintained paraformaldehyde at 2%.

Pre-embedding fluoronanogold labeling appears to work with the absence of glutaraldehyde in the fixative, however the
ultrastructure is quite compromised, as one would expect by TEM.

I have been thinking now of using LR-Gold, until I hear from some experts on any other suggestions. Thank you very much!
Vickie


Hi Vickie,

I had a similar situation and had successfully used pre-embed labelling
with FluoroNanogold. I hope I can help.
In the post-section fluoronanogold labelling, there might not
be enough cross linking of your proteins to keep them stabilized especially
during the dehydration and embedding steps. Antigen unmasking
might not be the issue there but rather the proteins have been
extracted. From what I recall lipids rely on osmium
tetroxide and urany acetate fixation. Assuming you have
abundant amount of these proteins, if you have already tried
the gradual low temperature approach during dehydration and resin
infiltration steps and there is still no labelling, I would try
another approach.

You mentioned that pre-embed fluoronanogold labelling worked but
the ultrastructure quality was quite compromised. That sounds promising
when you get similar labelling pattern as in previous light microscopy data.
In this case, you might be able to fine tune the fixation step and
permeabilization if the latter was used. In my experience,
this approach worked better for the Flower, a synaptic vesicle protein
in the neuromuscular junction (NMJ) which we were working on.
We tried several fixation combinations but found that overnight
4% paraformaldehyde fixation achieved sufficient labelling. Although
ultrastructure quality was not the same as routine TEM, the organelles
were still recognizable. We were a bit lucky because the NMJs lie close
to the muscle surface and go only as deep as 5-8 microns into the tissue.
Also, we worked on fillet-dissected third instar Drosophila larvae
and did not need 50 micron thick vivatome sections.
Even so, we still needed permeabilization steps but not as drastic
as used in regular immunofluorescence. After trying different
detergents and conc, we added 0.01% Tween 20 in the blocking step. The nice thing about using
FluoroNanogold is we can examine the fluorescence labelling before proceeding to next steps.
In addition, you can post fix with 3% Glut and use lower concentration
of osmium tetroxide prior to dehydration and embedding so the
sample quality should be more enhanced.

I am not an expert in Immunocytochemistry like Drs Paul Webster and
Jan Leunissen and others out there but one thing I learned
as I was getting into this is that there is no single labelling approach.
Pre-embed method is not suitable for all samples but would likely work
on cell monolayers/suspensions, vivatome sections and samples where antigen is easily accessible.
We try to fit the best immuno-EM method to your samples which we all know
usually takes time. I hope you have plenty of time to optimize the
method for your samples.

I will send a pdf of the Flower paper so that you can check the
micrographs. A detailed pre-embed method with Fluoronanogold
is on the supplemental material.

Good luck,

Claire

------------------------------------------------------------
Claire M. Haueter
Res. Tech. II (Electron Microscopy)
Bellen Lab
HHMI-Baylor College of Medicine
Jan and Dan Duncan Neurological Research Institute
1250 Moursund St. Suite 1125
Mailstop NR-1125
Houston, TX 77030
Phone: 832-824-8772
Fax: 832-825-1240
Email: chaueter-at-bcm.edu
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From: Fengxia.Liang-at-med.nyu.edu
Date: Tue, 25 Jan 2011 12:41:58 -0600
Subject: [Microscopy] Research Scientist Position in the Cryo-Electron Microscopy

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The New York Structural Biology Center (NYSBC) seeks an experienced electron
microscopist to join the staff of its Cryo-Electron Microscope Facility
(http://cryoem.nysbc.org). The NYSBC is shared center that supports
state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities
include four transmission electron microscopes and a new dual-beam scanning
electron microscope, which support projects involving electron tomography,
single particle analysis and electron crystallography of both stained and
frozen-hydrated samples. In general, projects focus on 3D reconstruction of
biological assemblies and examples range from the atomic structure of
membrane proteins, to the subunit organization in macromolecular complexes
and the cellular anatomy within the developing nematode. Implementation of
new technologies is an ongoing interest at NYSBC and, with the dual-beam
microscope, NYSBC plans to expand the scale of 3D reconstructions to
encompass the characterization of entire cells and their distributions
within their native tissue. To assist in these developments, NYSBC seeks a
individual with postdoctoral experience in biological electron microscopy
and image reconstruction. This individual will carry out experiments in
support of collaborative projects with affiliated investigators, including
EM sample preparation, data collection and image processing. Day-to-day
duties also include assisting a wide range of users from the NYSBCĻs ten
Member Institutions and setup and maintenance of microscopes. The individual
should be capable of multitasking and should enjoy working with other
people. Good communication skills are essential. Qualified applicants
should send a curriculum vitae and names of three references to David Stokes
(stokes-at-nysbc.org). Salary is commensurate with experience. The position is
currently open and applications will be reviewed continuously until the
position is filled.


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From: baskin-at-bio.umass.edu
Date: Tue, 25 Jan 2011 15:54:15 -0600
Subject: [Microscopy] Edwards Xenosput XE200 problem

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Microscopists,
I am posting this on behalf of my colleague Mike Jercinovic.
He is not a current subscriber so please be sure to cc him on any
reply. His email is: mjj-at-geo.umass.edu. Thanks! TB

We have recently acquired an old Edwards Xenosput XE200 magnetron
sputter coater. Does anyone have experience with these? It
certainly produces an amazing coat, but has a rather bothersome
problem. Often when we actuate the vacuum valve to start the initial
pump down, or sometimes when we close the valve, the display goes
haywire. The vacuum, head power, and head current readings all go
crazy. The vacuum valve is pretty aggressive, so the whole thing
gets a jolt when it opens or closes, so it seems like something might
just be loose somewhere. This behavior can (sometimes) go away when
switching the power off and on, or sometimes when cycling things a
few times, but as this is happening quite frequently, the coater is
almost unusable at this point. Today, it happened just when we
vented it to put samples in. We got a "parts" machine along with the
"working unit" (both obtained directly from Edwards), and I have now
replaced the switching relay, the display electronics, the control
electronics, and cables to the big board which has the terminal
blocks....no change in behavior. I have reset all the physical
interconnections I can find. Before launching in on further parts
swapping, I thought it would be worth an enquiry to the community.
Edwards no longer sells or supports this machine at all, so we are
basically on our own.

Thanks for any help. Mike Jercinovic. UMass Geosciences.

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From: gul417-at-mail.usask.ca
Date: Tue, 25 Jan 2011 20:14:33 -0600
Subject: [Microscopy] viaWWW: Edwards S150B sputter coater part: insulator/cable

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Email: gul417-at-mail.usask.ca
Name: Guosheng Liu

Organization: University of Saskatchewan

Title-Subject: [Filtered] Edwards S150B sputter coater part: insulator/cable

Message: Hi Steven,

Yes, the broken part is exactly as same as yours: at the bit on that
connects to the head terminal. More frustrating, I can tell that it
has broken at least 3 times from the record of usages--apparently a
design flaw.

I might try to sold them together according to suggestions from the
listers (I thank you all who replied to my email).

Best regards,

Guosheng Liu


-----------------------
Hi there;

I was in the exact same boat as you, except I have an S150A coater.
My HV cable broke (actually corroded and rotted away) right at the bit
on that connects to the sputter head terminal. I called Edwards and
spent a long time trying to even convince their service people that
they made this piece of equipment. I ended up having to fax them
copies of their own manual to show them the parts I was talking about.

Long story short: I never got anything from Edwards (useless). In the
end I crimped to the end of the cable an eye-lug terminal and we just
screw it onto the top of the sputter head now.

Best regards,
Steven Cogswell, P.Eng.
UNB Microscopy and Microanalysis

On Mon, Jan 24, 2011 at 8:15 PM, {gul417-at-mail.usask.ca} wrote:
} }
} }
} }
} }
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} } Organization: University of Saskatchewan
} }
} } Title-Subject: [Filtered] Edwards S150B sputter coater part:
} insulator/cable
} }
} } Message: Hello,
} }
} } The insulator cable in our aged Edwards S150B sputter coater broke
} } (so no HT) a few days ago. Does anyone have a clue on where can I
} } order this part (or a compatible part from other makes) or get a
} } service (I'm in Canada)?
} }
} } Thanks in advance.
} }
} } Guosheng
} }


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From: Artur.irani-at-yahoo.com
Date: Wed, 26 Jan 2011 07:55:46 -0600
Subject: [Microscopy] viaWWW: Beam problem EPMA SX100

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Email: Artur.irani-at-yahoo.com
Name: Kazem

Organization: IMPRC

Title-Subject: [Filtered] Beam problem

Message: Hi
I am an EPMA SX100 Operator and it has a unknown problem during 2
weeks. when i choose HV(Kv) it getting on but HV is always became 0 ,
I checked the everywhere and changed Filament, also there is no
error. but how much I try and give Hv it never changed. please help
me in this situation.
Kazem

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From: protrain-at-emcourses.com
Date: Wed, 26 Jan 2011 08:57:52 -0600
Subject: [Microscopy] viaWWW: Beam problem EPMA SX100

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Hi

This does not look like a self fix task as it seems that you have a problem
with your high voltage system itself that is why it will not switch on. The
only other possible reason is that the safety device which prevents the HT
switching on under a poor vacuum could be at fault?

One question does the emission/beam current meter alter at all when you
switch on the HT? If it does it is not the safety device.

Steve

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To: protrain-at-emcourses.com

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Email: Artur.irani-at-yahoo.com
Name: Kazem

Organization: IMPRC

Title-Subject: [Filtered] Beam problem

Message: Hi
I am an EPMA SX100 Operator and it has a unknown problem during 2
weeks. when i choose HV(Kv) it getting on but HV is always became 0 ,
I checked the everywhere and changed Filament, also there is no
error. but how much I try and give Hv it never changed. please help
me in this situation.
Kazem

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6, 22 -- To: microscopy-at-microscopy.com
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From: DOrloff-at-ascb.org
Date: Wed, 26 Jan 2011 09:12:03 -0600
Subject: [Microscopy] The Cell: An Image Library - LinkedIn group

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


For those interested in cellular microscopy there is now The Cell: An Image Library - http://www.cellimagelibrary.org/

Also feel free to join The Cell LinkedIn Group - http://www.linkedin.com/groupRegistration?gid=3733425

David

David Orloff
Manager, Image Library
The American Society for Cell Biology
8120 Woodmont Avenue, Suite 750
Bethesda, MD 20814-2762
T: 301-347-9300/Direct: 301-347-9305
F: 301-347-9310
E-mail:† dorloff-at-ascb.org
Web site: www.ascb.org
The Cell: An Image LibraryT: www.cellimagelibrary.org
LinkedIn Group: http://www.linkedin.com/groupRegistration?gid=3733425




==============================Original Headers==============================
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From: benada-at-biomed.cas.cz
Date: Thu, 27 Jan 2011 04:37:19 -0600
Subject: [Microscopy] CM100 trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
We have another problem with our Philips CM100. Yesterday afternoon, the operating system of the
microscope crashed with strange OPCON screen (see the link bellow for image). No beeps, no
buttons were working with exception of the "OFF button". After restart, CM100 started in
‚ÄúDefault RAM init‚ÄĚ mode.

Please, did anybody observed something similar? In our case it was for the first time.
Any responses are welcome.

Image of OPCON screen after crash:
http://www2.biomed.cas.cz/~benada/CM100_trouble.html

Best regards
Oldrich

--------------------------
Oldrich Benada
Institute of Microbiology, Acad. Sci. CR
Videnska 1083
142 20 Prague 4
Czech Republic


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From: hstahlberg-at-me.com
Date: Fri, 28 Jan 2011 04:36:31 -0600
Subject: [Microscopy] How close can a NMR machine be installed next to a TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Hi,

How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?

I would appreciate any experience or recommendations, also directly towards me.

Thanks,

Henning.

___________________________________________________

Henning Stahlberg, PhD.
Center for Cellular Imaging and Nano Analytics (C-CINA)
Prof. for Structural Biology
Biozentrum, University Basel
at the Department of Biosystems Science and Engineering (D-BSSE)
Mattenstrasse 26, WRO-1058
CH-4058 Basel, Switzerland
Tel: +41-61-387 32 62 (office)
Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant)
Fax: +41-61-387 39 86
mailto:Henning.Stahlberg-at-unibas.ch
Skype:henningstahlberg
http://c-cina.org
http://2dx.org

Postal Address for delivery of Letters / Goods / Express Carriers:
C-CINA, Biozentrum University of Basel
c/o Syngenta AG, WRO-1058.6.60, D-BSSE
Schwarzwaldallee 215
Postfach
CH-4002 Basel
___________________________________________________

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From: microscopy-at-microscopy.com
Date: Fri, 28 Jan 2011 06:50:18 -0600
Subject: [Microscopy] viaWWW:LM: Navitar Tube Lens for use with Infinity Corrected Objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying please copy both wxufocus-at-gmail.com as well as the
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Email: wxufocus-at-gmail.com Name: Jizhong

Title-Subject: [Filtered] LM: Navitar Tube Lens for use with Infinity
Corrected Objective

Message: Hi, I'm setting up an optical system with an infinity
corrected objective lens in a special tool I'm designing. I'm thinking
of using an adapter tube from Navitar to form the image for a CCD
camera. The Adapter Tube has a nice C-mount for attaching the CCD
camera. My question is: is the tube lens (to convert the parallel beams
into a real image) resides inside the Adapter Tube? Or I need the Ultra
Body Tube? Please see the sys diagram:
http://machinevision.navitar.com/pdfs/Precise_Eye_System_Diagram.pdf

Thanks,

Jizhong
Login Host: 121.235.16.17
---------------------------------------------------------------------------



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From: Samuel.Connell-at-STJUDE.ORG
Date: Fri, 28 Jan 2011 06:57:10 -0600
Subject: [Microscopy] Out of Office: viaWWW:LM: Navitar Tube Lens for use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am no longer the Director of Light Microscopy at St. Jude Children's Research Hospital. For questions regarding the use of the Cell and Tissue Imaging Center, please direct your inquiries to the team of Imaging Scientists in the CTIC, Jennifer Peters, Yannan Ouyang, and Jamshid Temirov. They may be reached as a group at the following email address: lightmicroscopy-at-stjude.org. The phone number in the CTIC is 901-595-3439.

Although no longer serving in my former capacity as the Director of Light Microscopy, I am currently available as a technical consultant to the Cell and Tissue Imaging Center. I may be reached directly as necessary at the following email address: samuel.connell-at-gmail.com

Kindest Regards,
--
Samuel Connell

________________________________
Email Disclaimer: www.stjude.org/emaildisclaimer


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From: Jeff.Wagner-at-cdph.ca.gov
Date: Fri, 28 Jan 2011 07:00:34 -0600
Subject: [Microscopy] Out of Office AutoReply: viaWWW:LM: Navitar Tube Lens for use with Infinity Corrected Objective

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I will be out of the office January 28. I will return on Monday January 31.
-Jeff



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From: Earnest.Truby-at-MyFWC.com
Date: Fri, 28 Jan 2011 07:02:10 -0600
Subject: [Microscopy] Out of Office: viaWWW:LM: Navitar Tube Lens for use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am currently out of the office on extended leave. For assistance, please contact Mary Arnold (Mary.Arnold-at-MyFWC.com) and she will direct your inquiry to the appropriate staff member.

Earnest Truby



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From: mirandan-at-mail.nih.gov
Date: Fri, 28 Jan 2011 07:03:30 -0600
Subject: [Microscopy] Out of Office: viaWWW:LM: Navitar Tube Lens for use

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Hello,

Thank you for your message. Please note that I will be out of the office for the rest of today, Thursday, January 27th. During this time I will have no access to voice mail or e-mail. I will respond to all messages accordingly upon my return to the office tomorrow Friday, January 28th.

If your matter is urgent and requires IMMEDIATE attention, please contact our main employment line at 301-846-5362 and the HR Assistant will be happy to connect you with the appropriate recruiter.

Please note that on occasion, the opening of Ft. Detrick may be delayed or closed due to inclement weather. For any weather related concerns and additional information about delays/closings, you may contact the Fort Detrick Weather Alert Hotline at 301-619-7611.

Thank you for your understanding. Make it a great day!

Regards,
Nelmarie Miranda, PHR
Senior Employment Specialist
SAIC-Frederick, Inc.


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From: snezana.haymes-at-us.army.mil
Date: Fri, 28 Jan 2011 07:05:43 -0600
Subject: [Microscopy] Out of Office: viaWWW:LM: Navitar Tube Lens for use

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I will be out of office from Tuesday, January 25 until Friday, January 28. I will be back in my office on Monday, January 31.



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From: mike.bode-at-resaltatech.com
Date: Fri, 28 Jan 2011 07:06:25 -0600
Subject: [Microscopy] Mike Bode is out of the office. Re: viaWWW:LM: Navitar Tube Lens for use with Infinity Corrected Objective

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Dear microscopy-at-microscopy.com,

I will be out of the office from 1/20/11 - 1/28/11, and will have only limited access to my email. I will respond to your email when I am back in the office. In urgent cases, please contact Jason Wickersham at Jason.Wickersham-at-resaltatech.com, or call (551)-804-1845.

Thank you for your understanding.

Mike Bode
ResAlta Research Technologies

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From: benada-at-biomed.cas.cz
Date: Fri, 28 Jan 2011 07:27:18 -0600
Subject: [Microscopy] Re: CM100 trouble - solved (hopefully)

Contents Retrieved from Microscopy Listserver Archives
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Hello,
Many thanks for all advices and suggestions. The most likely it was a power spike that caused
the problem. Now, our CM100 is working again. Unfortunately, we have only DOS remote control
package for microscope setting backup and our old DOS PC does not boot any more. It took me half
a day to align and recalibrate the microscope manually after its RAM init. I'm thinking about
setup a virtual box with DOS and remote control package in near future.

I merged together all the replies and they are available on following link:
http://www2.biomed.cas.cz/~benada/CM100_trouble_r.html

Many thanks again Oldrich

} } Hello,
} } We have another problem with our Philips CM100. Yesterday afternoon, the
} } operating system of the microscope crashed with strange OPCON screen
} } (see the link bellow for image). No beeps, no buttons were working with
} } exception of the "OFF button". After restart, CM100 started in ďDefault
} } RAM initĒ mode.
} }
} } Please, did anybody observed something similar? In our case it was for
} } the first time. Any responses are welcome.
} }
} } Image of OPCON screen after crash:
} } http://www2.biomed.cas.cz/~benada/CM100_trouble.html
} }
} } Best regards
} }
} } Oldrich
} }
} } --------------------------
} } Oldrich Benada
} } Institute of Microbiology, Acad. Sci. CR
} } Videnska 1083
} } 142 20 Prague 4
} } Czech Republic


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From: zaluzec-at-aaem.amc.anl.gov
Date: Fri, 28 Jan 2011 07:34:49 -0600
Subject: [Microscopy] Administrivia: Email Loop Created - do not reply to any Out-of-Office Messages

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Colleagues

In trying to update some software I inadvertently created an email loop.
You all will see a number of out-of-the-office messages.

Do not reply to these and please excuse my error. Just ignore them
hopefully the loop will clear in a short while.


Nestor
Your Friendly Neighborhood SysOp... who also makes mistakes

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From: colijn.1-at-osu.edu
Date: Fri, 28 Jan 2011 07:46:43 -0600
Subject: [Microscopy] Re: How close can a NMR machine be installed next to a TEM

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Hi Henning,

I'm not sure of the distance, but you should be able to use a cheap
gaussmeter to measure the fields from the NMR. Even a DC field can
cause problems for a TEM, particularly a high-end FEG TEM. Anyone who
has worked with magnetic samples can attest to the problems they cause.

While there may be strong DC fields, NMRs work by flipping nuclear
magnetic moments which suggests there are AC or pulsed components to the
field.

You do not want this beast close to your TEM column.

Cheers,
Henk

At 1/28/2011 5:37 AM, hstahlberg-at-me.com wrote:
}
} ----------------------------------------------------------------------------
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}
} Hi,
}
} How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?
}
} I would appreciate any experience or recommendations, also directly towards me.
}
} Thanks,
}
} Henning.
}
} ___________________________________________________
}
} Henning Stahlberg, PhD.
} Center for Cellular Imaging and Nano Analytics (C-CINA)
} Prof. for Structural Biology
} Biozentrum, University Basel
} at the Department of Biosystems Science and Engineering (D-BSSE)
} Mattenstrasse 26, WRO-1058
} CH-4058 Basel, Switzerland
} Tel: +41-61-387 32 62 (office)
} Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant)
} Fax: +41-61-387 39 86
} mailto:Henning.Stahlberg-at-unibas.ch
} Skype:henningstahlberg
} http://c-cina.org
} http://2dx.org
}
} Postal Address for delivery of Letters / Goods / Express Carriers:
} C-CINA, Biozentrum University of Basel
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} Schwarzwaldallee 215
} Postfach
} CH-4002 Basel
} ___________________________________________________
}
} ==============================Original Headers==============================
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} 10, 23 -- From: Henning Stahlberg {hstahlberg-at-me.com}
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}

--
Hendrik O. Colijn www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
Columbus, OH 43210

"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

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From: David.Patton-at-uwe.ac.uk
Date: Fri, 28 Jan 2011 09:26:18 -0600
Subject: [Microscopy] How close can a NMR machine be installed next to a TEM

Contents Retrieved from Microscopy Listserver Archives
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We have a Philips CM10 TEM. Jeol checked for problems prior to installing a Jeol Eclipse 300MHz superconducting magnet instrument 13m and two rooms away. As far as we know there was no interference.

Dave

-----Original Message-----
X-from: hstahlberg-at-me.com [mailto:hstahlberg-at-me.com]
Sent: 28 January 2011 10:40
To: David Patton



Hi,

How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year old NMR machine? If both are on the same floor, then the magnetic field lines from the NMR should run through the TEM column vertically, so that the sensitivity for the strong DC fields of the NMR machine might be less ?

I would appreciate any experience or recommendations, also directly towards me.

Thanks,

Henning.

___________________________________________________

Henning Stahlberg, PhD.
Center for Cellular Imaging and Nano Analytics (C-CINA)
Prof. for Structural Biology
Biozentrum, University Basel
at the Department of Biosystems Science and Engineering (D-BSSE)
Mattenstrasse 26, WRO-1058
CH-4058 Basel, Switzerland
Tel: +41-61-387 32 62 (office)
Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant)
Fax: +41-61-387 39 86
mailto:Henning.Stahlberg-at-unibas.ch
Skype:henningstahlberg
http://c-cina.org
http://2dx.org

Postal Address for delivery of Letters / Goods / Express Carriers:
C-CINA, Biozentrum University of Basel
c/o Syngenta AG, WRO-1058.6.60, D-BSSE
Schwarzwaldallee 215
Postfach
CH-4002 Basel
___________________________________________________

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From: raristau-at-ims.uconn.edu
Date: Fri, 28 Jan 2011 11:02:54 -0600
Subject: [Microscopy] Re: How close can a NMR machine be installed next to

Contents Retrieved from Microscopy Listserver Archives
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Part of our microscopy lab is next door to the NMR lab. The high
electro-magnetic fields (presumably from the high voltage lines feeding the
NMR) preclude installation of SEM or TEM in areas near the adjoining wall. A
distance of only a few meters is sufficient for the field strength to drop
to safe levels.

We have a similar problem with the HRTEM (located ~30 meters from the NMR
lab) in that a major high voltage conduit is located in the ceiling. As the
EMF will drop with the square of the distance, the ~2 meter separation from
conduit to TEM column is (just) sufficient to permit unimpeded operation.

The main point to take away from this is that any space MUST be checked for
fields and vibrations before installing any microscope. This should be
required by the TEM maker. There may be sources of EMF that are not obvious.

Cheers
Roger

Roger A. Ristau, PhD
Electron Microscopy Specialist
Institute of Materials Science
97 North Eagleville Road
University of Connecticut
Storrs, CT 06269
vox: 860-486-5453
fax: 860-486-4745


} From: {hstahlberg-at-me.com}
} Reply-To: {hstahlberg-at-me.com}
} Date: Fri, 28 Jan 2011 04:40:50 -0600
} To: {raristau-at-ims.uconn.edu}
} Subject: [Microscopy] How close can a NMR machine be installed next to a TEM
}
}
}
}
} ----------------------------------------------------------------------------
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} Hi,
}
} How close can a high-end 300kV FEG TEM be installed next to an 800MHz 20-year
} old NMR machine? If both are on the same floor, then the magnetic field lines
} from the NMR should run through the TEM column vertically, so that the
} sensitivity for the strong DC fields of the NMR machine might be less ?
}
} I would appreciate any experience or recommendations, also directly towards
} me.
}
} Thanks,
}
} Henning.
}
} ___________________________________________________
}
} Henning Stahlberg, PhD.
} Center for Cellular Imaging and Nano Analytics (C-CINA)
} Prof. for Structural Biology
} Biozentrum, University Basel
} at the Department of Biosystems Science and Engineering (D-BSSE)
} Mattenstrasse 26, WRO-1058
} CH-4058 Basel, Switzerland
} Tel: +41-61-387 32 62 (office)
} Tel: +41-61-387 32 31 (Karen Bergmann, administrative assistant)
} Fax: +41-61-387 39 86
} mailto:Henning.Stahlberg-at-unibas.ch
} Skype:henningstahlberg
} http://c-cina.org
} http://2dx.org
}
} Postal Address for delivery of Letters / Goods / Express Carriers:
} C-CINA, Biozentrum University of Basel
} c/o Syngenta AG, WRO-1058.6.60, D-BSSE
} Schwarzwaldallee 215
} Postfach
} CH-4002 Basel
} ___________________________________________________
}
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From: contact-at-excaliburpathology.com
Date: Fri, 28 Jan 2011 11:42:57 -0600
Subject: [Microscopy] Yahoo! Auto Response

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I will be out of the lab Jan. 22 to Jan 30, 2011.
In case of emergency, please call 405-759-3953 and someone will get in touch with me.

Thank you,

Paula Pierce

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From: jfmjfm-at-umich.edu
Date: Fri, 28 Jan 2011 12:25:29 -0600
Subject: [Microscopy] How close can a NMR machine be installed next to a

Contents Retrieved from Microscopy Listserver Archives
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I would be interested in a summary of the answers received on this.
We have a new instrument that may be installed within 200feet of an older model Varian MRI system, it is a superconductor, and the magnet is 4.7T. I do not believe that it is shielded the way the more modern MRIs are so I am curious what the field is out at 200ft from the instrument. I have the expressions for the fields from a solenoid at a distance, but I would rather have measurements than guesstimates, particularly when the kinds of static fields that we wish to avoid are probably in the milligauss or better regime.
I will probably find an instrument (MRI) is operational and plot the fields as a function of both distance and angle to the principal axis of the MRI.


--
John Mansfield PhD Cphys MInstP
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352 FAX (734) 763-2282
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Home address:
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Please note: Electronic Mail is not secure, but should be read several times every day, and should definitely be used for urgent or sensitive issues.






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From: dmywong-at-ucdavis.edu
Date: Fri, 28 Jan 2011 14:36:10 -0600
Subject: [Microscopy] CLSM: Dying Triglyceride of microalgae

Contents Retrieved from Microscopy Listserver Archives
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I have a hard time getting the triglycerides to dye in 1 micron green
algae due to their thick cell wall. Any suggestions? I plan to view
this on a confocal laser scanning microscope.

Thanks!




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From: dmywong-at-ucdavis.edu
Date: Fri, 28 Jan 2011 14:41:44 -0600
Subject: [Microscopy] Confocal LSM: Quantification software? ImageJ

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I am trying to do quantification of my cells by measuring the diameter
of the fluorescence from a 3D construction image. I have heard of
ImageJ, but I am not sure what pluggins to install. Have you tried a
better program?
______________________________
Diana M. Wong
Graduate Student

Franz Group
University of California, Davis
Department of Chemistry
One Shields Ave
Davis, CA 95616
dmywong-at-ucdavis.edu
http://chemgroups.ucdavis.edu/~franz/





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From: michael.anderson-at-nist.gov
Date: Fri, 28 Jan 2011 15:13:10 -0600
Subject: [Microscopy] Confocal LSM: Quantification software? ImageJ

Contents Retrieved from Microscopy Listserver Archives
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Diana,

Rather than going with the a la carte method of installing plugins for ImageJ* you could try one of the prepackaged collections. MBF ImageJ is a good prepackaged selection of plugins (http://www.macbiophotonics.ca/imagej/) You could also try Fiji (http://pacific.mpi-cbg.de/wiki/index.php/Fiji).

Regards,

Mike

(*Comments made above represent personal opinion and do not represent formal endorsement of any particular product, company, or organization by NIST.)


-----Original Message-----
X-from: dmywong-at-ucdavis.edu [mailto:dmywong-at-ucdavis.edu]
Sent: Friday, January 28, 2011 3:58 PM
To: Anderson, Michael

I am trying to do quantification of my cells by measuring the diameter
of the fluorescence from a 3D construction image. I have heard of
ImageJ, but I am not sure what pluggins to install. Have you tried a
better program?
______________________________
Diana M. Wong
Graduate Student

Franz Group
University of California, Davis
Department of Chemistry
One Shields Ave
Davis, CA 95616
dmywong-at-ucdavis.edu
http://chemgroups.ucdavis.edu/~franz/





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From: zaluzec-at-aaem.amc.anl.gov
Date: Sat, 29 Jan 2011 09:53:54 -0600
Subject: [Microscopy] Administrivia: Testing today

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Colleagues

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From: microscopylistserver-noreply-at-microscopy.com
Date: Sat, 29 Jan 2011 14:24:35 -0600
Subject: [Microscopy] viaWWW:Re: How close can a NMR machine be installed next to

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Email: reynolds-at-vt.edu Name: Bill Reynolds

Organization: Virginia Tech

Title-Subject: [Microscopy] Re: How close can a NMR machine be installed
next to

Message: We have a FEG-STEM with a GIF that is located about 30 meters
away from an NMR lab. Our microscope operates to the microscope
manufacturer's resolution specs.
As others have noted, what causes problems for TEMs are magnetic fields
that change relatively quickly. These come from things like power lines,
large motors, and electromagnetic contacts or switches. Static fields
do not matter much at all because you can compensate for them (even when
the field is associated with a ferromagnetic sample in the microscope).
When our microscope was installed, the manufacturer measured the stray
magnetic fields (the alternating fields that are easy to detect), but I
don't think they even bothered measuring the static field. As an aside,
the natural static field at the earth's surface is small, but it is not
insignificant. Its magnitude varies from place to place, but it is in
the vicinity of half a Gauss. All microscopes essentially work in a 500
milli Gauss static background.

The large fields associated with NMR instruments are also static, so
they probably should not be considered equivalent to the "stray EMF
fields" discussed in various microscope installation specs. In our lab,
the magnet is a superconducting coil, and the weak fringes of the
magnetic field extends to our microscope. However, since the field
doesn't change (and we don't distort it by moving around large chunks of
iron), our microscope does not notice the presence of the NMR. If the
NMR magnet ever quenches or is turned off, we will probably be able to
see a very small change in the microscope alignment, but that kind of
event has not happened yet because the NMR magnet has been on
continuously for a couple years.

Just one opinion,
Bill Reynolds

Login Host: 198.82.166.169
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From: kjmorris-at-well.ox.ac.uk
Date: Sat, 29 Jan 2011 19:02:37 -0600
Subject: [Microscopy] RE: Confocal LSM: Quantification software? ImageJ

Contents Retrieved from Microscopy Listserver Archives
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Hi Diana,

I assume, rather than a simple chord on a 2D z slice, you want to measure
a line between two points on different Z planes in the 3D z stack. If so
also try and track down a recent version of the 3D software Volocity from
Perkin Elmer [formally Improvision], I am sure there will be a few
licences for the software about in one of the microscopy cores or research
groups at the university. Volocity is pretty hot at 3D quantification,
e.g. cellular component volumes etc.., and it shows them as pretty
pictures & videos. Although expensive to buy, Volocity works well, gives
good feedback on what it was measured, and has excellent email and
telephone support in the UK, and I expect the same is true in the US. You
will need to find a licenced user with at least the visualisation module,
and probably the quantitation module [i.e. drawing tools], particularly if
you want to take the image analysis further [these cost extra and not all
users will have all the modules].

http://www.cellularimaging.com/products/volocity
http://www.well.ox.ac.uk/_asset/file/volocity-manual.pdf [see line tool]
http://www.cellularimaging.com/lab_of_the_month/detail.php?id=12

I'm sure there will be other microscope software that other Californian
University groups may have that can do this as well, including ImageJ
[although I can't think of a 3D line distance measurements ImageJ app,
more volume rendering, but check the Fuji version], MetaMorph [also only
available via an optional 3D module] and Image-Pro Plus [3D rendering and
measurement].

If you find your XY pixel cordinates of the first and last point in the 3D
confocal z-stack and know your approx. optical slice thickness and number
of slices, I guess you could calculate/estimate the connecting line length
pretty easily [you'd convert everything to pixels and then back to um].
There'd be errors in the length measurement, e.g. owing to perhaps 10 to
25% reduction in size due to fixation, possible distortion effects and
estimation of Z optical distances.

e.g. calculate using
http://www.analyzemath.com/Geometry_calculators/distance_midpoint_3D.html

Also see: Microscopy Image Analysis and Processing Software links at:
http://www.well.ox.ac.uk/external-website-links

Regards

Keith

No commercial interest

-------------------------------------------------

Dr Keith J Morris
Molecular Cytogenetics and Microscopy Core
The Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford
OX3 7BN
United Kingdom

Tel: +44 ( 0 ) 1865 287568
Email: kjmorris-at-well.ox.ac.uk
HomePage: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy

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} Diana,
}
} Rather than going with the a la carte method of installing plugins for
ImageJ* you could try one of the prepackaged collections. MBF ImageJ is
a
} good prepackaged selection of plugins
} (http://www.macbiophotonics.ca/imagej/) You could also try Fiji
(http://pacific.mpi-cbg.de/wiki/index.php/Fiji).
}
} Regards,
}
} Mike
}
} (*Comments made above represent personal opinion and do not represent
formal endorsement of any particular product, company, or organization
by
} NIST.)
}
}
} -----Original Message-----
} X-from: dmywong-at-ucdavis.edu [mailto:dmywong-at-ucdavis.edu]
} Sent: Friday, January 28, 2011 3:58 PM
} To: Anderson, Michael
} Subject: [Microscopy] Confocal LSM: Quantification software? ImageJ
}
}
}
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}
} I am trying to do quantification of my cells by measuring the diameter
of the fluorescence from a 3D construction image. I have heard of
ImageJ, but I am not sure what pluggins to install. Have you tried a
better program?
} ______________________________
} Diana M. Wong
} Graduate Student
}
} Franz Group
} University of California, Davis
} Department of Chemistry
} One Shields Ave
} Davis, CA 95616
} dmywong-at-ucdavis.edu
} http://chemgroups.ucdavis.edu/~franz/
}
}
}
}
}
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From: Michael.Cammer-at-med.nyu.edu
Date: Sun, 30 Jan 2011 19:25:32 -0600
Subject: [Microscopy] how to induce apoptosis for in vitro microscopy?

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Dear microscopists,
I have been asked to reproduce an apoptosis assay whereby cells in a dish cells are exposed to UVB light and then screened 8 to 36 hours later.
Does anybody have experience with this who could give us some tips?
May we use UVA, as in the UV lamp on the microscope, or do you have suggestions what type of lab might have a UVB lamp available?
Thank you.
Sincerely,
Michael


_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270

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From: Stefan.Heinemann-at-fei.com
Date: Mon, 31 Jan 2011 19:43:48 -0600
Subject: [Microscopy] Automatic reply: viaWWW:LM: Navitar Tube Lens for use

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I am out of the office till the 18. of July.


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From: stefan.diller-at-t-online.de
Date: Tue, 1 Feb 2011 06:20:07 -0600
Subject: [Microscopy] SEM prep of lichen from a herbarium

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
I would like to do some work on lichen. Now I got access to a huge herbarium collection of lichen.
What it do any good to use these room-dried specimen, moisture them, fix and go through ethanol to critcal point drying?
Any experience with this out there?

Best wishes,
Stefan

--
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 1 Feb 2011 08:41:26 -0600
Subject: [Microscopy] viaWWW:EMAG2011 - Abstract Submission Open

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Email: ian.maclaren-at-glasgow.ac.uk Name: Ian MacLaren

Organization: University of Glasgow

Title-Subject: [Filtered] EMAG2011 - Abstract Submission Open

Message: EMAG2011 - Quantifying the Nanoworld
September 6-9 2011
University of Birmingham, UK

Call for abstracts

The biennial EMAG conference will this year be held at the University of
Birmingham and promises to be an excellent meeting. Highlights of the
programme will include plenary talks from Dr Frances Ross, Prof. Knut
Urban and Prof. Richard Henderson, together with an exciting range of
invited talks giving a snapshot of many current directions in electron
microscopy and its applications. There will also be plenty of
opportunity at this meeting to present papers orally or as posters, and
research students are especially encouraged to present their work at
this meeting. Scientific sessions are expected to include:

* Nanofabrication and scanning electron microscopy
* Modelling and quantification
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* Applications of high resolution imaging and spectroscopy
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* Earth and environmental sciences
* In-situ electron microscopy
Abstract submission is now open via the conference website at:

http://www.emag-iop.org/
and the closing date for abstract submission is 2 March 2011. Further
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 1 Feb 2011 08:42:59 -0600
Subject: [Microscopy] viaWWW:Postdoctoral Research Position Youngstown State University

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Email: vcsolomon-at-ysu.edu Name: Virgil C. Solomon

Organization: Youngstown State University

Title-Subject: [Filtered] Postdoctoral Research Position

Message: A postdoctoral research position is available at Youngstown
State University to study ceramic-metallic composites obtained by
reactive melt infiltration. More specifically, the successful candidate
will use transmission and scanning electron microscopy methods to
characterize micro- and nanostructural features of the composite
materials. Candidates should have a Ph.D. in materials science,
chemistry, physics or related engineering discipline. The position
requires extensive experience in analytical scanning and transmission
electron microscopy and sample preparation techniques; additional
experience working with focused ion beam is preferred. Responsibilities
of the successful candidate will include planning and performing
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scientific reports and journal publications. This position provides the
opportunity to work in a new, fully-equipped, state-of-the-art electron
microscopy facility which opened at YSU in 2010.

More information available at http://www.ysu.edu/hr/positions.shtml

Interested candidates can apply by email or by sending a cover letter,
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 1 Feb 2011 09:13:38 -0600
Subject: [Microscopy] viaWWW:Cryostat Knife Holder Assembly Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stefan

it's not my particular area of expertise but I do recall that some of
our researchers used to "revitalise" their dried lichen samples by
simply putting them on a damp filter paper in a petri-dish. If you
think about it, this is probably what happens in nature (except for
the filter paper etc).

I seem to recall that this process worked with most specimens but
depended on the original drying, storage and presumably the resilience
of the fungi and algae symbionts.

Good luck

Malcolm

Malcolm Haswell
Imaging Suite
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk

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Email: steven.samuelsson-at-sri.com Name: Steven Samuelsson
Organization: SRI International, Inc.

Title-Subject: [Filtered] Cryostat Knife Holder Assembly Needed

Message: We recently acquired a vintage Reichert- Jung, Cryocut 1800.
Unfortunately, it is missing much of the knife holder assemble. Does
anyone on the listserver have these parts and are willing to sell or
surplus them? The unit is in good condition and should serve us well.
Thanks so much.

Steve
-- Steven Samuelsson, PhD
Director
Cell and Molecular Imaging Facility
100-51
SRI International, Inc.
333 Ravenswood Ave
Menlo Park, CA 94061
(650) 859-2980

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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 1 Feb 2011 09:15:23 -0600
Subject: [Microscopy] viaWWW:Type K(?) insert for Zeiss

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Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer

Organization: Skirball Institute of Biomolecular Medicine

Title-Subject: [Filtered] Type K(?) insert question

Message: We want to built a custom type K (?), 110 X 160 mm with
rounded corners, insert for our Zeiss microscope stage that will allow
us to insert another stage insert approximately 2 cm higher than the
current stage.

Does anyone know where we can get drawings of the insert that we modify
for a machine shop?

Or does anyone know if such a stage height extender already exists?

Thank you very much.

Sincerely,

Michael

________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
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From: eschumacher-at-mccrone.com
Date: Tue, 1 Feb 2011 09:42:05 -0600
Subject: [Microscopy] Short Course Announcement: SEM and TEM

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Greetings Fellow Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering the following electron microscopy short courses:

March 7 to 11- Scanning Electron Microscopy

March 15 to 17 - Transmission Electron Microscopy

In addition to lectures, these courses emphasize hands-on training using state of the art equipment. For further details and registration information, please follow the link below:

http://www.hookecollege.com/


Regards,

Elaine

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com



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From: baskin-at-bio.umass.edu
Date: Tue, 1 Feb 2011 09:50:13 -0600
Subject: [Microscopy] Re:SEM prep of lichen from a herbarium

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Dear Stefan,
I have no specific experience with lichen but I suspect that
whatever structural damage occurred by air drying cannot be repaired
by rehydration. I bet you can take small sample of the herbarium
specimen and put it on a stub and examine directly. I think you can
test it first in a vacuum chamber (for example of a good coater) and
see if it outgasses much. Probably it won't. Insofar as lichens air
dry in nature and plant and fungal cell walls are tough, it is
reasonable to examine air dried material in lieu of fresh samples.

Clearly if as Malcolm Haswell suggests the herbarium specimen
is still living, then that gives you the chance to work with fresh
material. But I wonder what the odds of that are?

As ever
Tobias Baskin

}
}
} Dear All,
} I would like to do some work on lichen. Now I got access to a huge
} herbarium collection of lichen.
} What it do any good to use these room-dried specimen, moisture them,
} fix and go through ethanol to critcal point drying?
} Any experience with this out there?
}
} Best wishes,
} Stefan
}
} --
} -----------------------------------------------------
} Stefan Diller - Scientific Photography
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49-931-7848700 Phone
} ++49-931-7848701 Fax
} ++49-175-7177051 Mobile
}
} Websites:
} www.stefan-diller.com
} www.elektronenmikroskopie.info
} www.assisi.de
} www.zwillingsprojekt.de
} Anfahrt: //Mail.map24.com/Stefan.Diller
} -----------------------------------------------------
}
} =

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Biology Department
/_ / __ /__ \ \ \__ 611 N. Pleasant St.
/ / / \ \ \ University of Massachusetts
/ / / \ \ \ Amherst, MA, 01003
/ / ___ / \ \__/ \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

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From: FMonson-at-wcupa.edu
Date: Tue, 1 Feb 2011 09:53:31 -0600
Subject: [Microscopy] SEM prep of lichen from a herbarium

Contents Retrieved from Microscopy Listserver Archives
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Morning Stephan,

We often neglect to consider that when something has already been dried 'naturally' there may be no reason the redo the process 'un-naturally'. Especially in the cases of organisms that can survive dehydration by re-animating when re-hydrated. There is a roundworm in S. America that survives complete dessication in the dried mud - waiting for reanimation in the next rainy season. (Could NOT quickly find a reference, but I first saw this in a text - when I was young - in the early 1960's.)

This is especially true, if you have a variable pressure SEM (ESEM) at hand.

Further, after critical point drying, we also fail to recognize that the 'almost completely dry' natural material has been remade into a quite hygroscopic material that will rehydrate while we carry it to the microscope or coater (evacuate (shrinkage) and coat, then re-pressurize, hydrate and stretch!). All of this was invented to permit us to view free-standing microvilli as well as other delicate cell surface features.

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl


-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: Tuesday, February 01, 2011 7:32 AM
To: Monson, Frederick

Dear All,
I would like to do some work on lichen. Now I got access to a huge herbarium collection of lichen.
What it do any good to use these room-dried specimen, moisture them, fix and go through ethanol to critcal point drying?
Any experience with this out there?

Best wishes,
Stefan

--
-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

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From: beth-at-plantbio.uga.edu
Date: Tue, 1 Feb 2011 13:55:21 -0600
Subject: [Microscopy] fixing mouse cornea

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Hi all,
Does anyone have a favorite TEM protocol for fixing mouse cornea that
they'd be willing to share? The tissue we have is infected with a
fungus so we'd like to have good preservation of both.
TIA and my best,
Beth

Beth Richardson
EM Lab Coordinator
Plant Biology Dept.
University of Georgia
Athens, GA 30602




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From: tina-at-pbrc.hawaii.edu
Date: Tue, 1 Feb 2011 15:06:27 -0600
Subject: [Microscopy] Need a chapter on EM for a book

Contents Retrieved from Microscopy Listserver Archives
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Hi, All-

The editor of a forthcoming Wiley-Blackwell book on Biofouling Methods is
looking for someone to write a chapter on electron microscopy methods.
This is expected to be a brief literature review, with an introduction
about methods and some actual protocols, designed for beginners in the
field. If you are interested, please contact Sergey Dobretsov at
sergey_dobretsov-at-yahoo.com

Aloha from sunny and warm Hawaii (had to rub it in),
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: rosemary.white-at-csiro.au
Date: Tue, 1 Feb 2011 15:34:53 -0600
Subject: [Microscopy] Re: SEM prep of lichen from a herbarium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

As suggested already, if you have access to a VP-SEM, all you need to do is
put a piece on a stub and image. And sometimes BSE is better than SE, or a
combination of the two can work well. And in a VP-SEM, you don't need to
worry about outgassing so much.

After that, you could try looking at a hydrated specimen in VP mode, with
water as the gas, if your SEM has that capacity.

The entomologists across the road are big fans of the VP-SEM, because it
means they can now look at details of their precious type specimens,
especially the very tiny ones, none of which are allowed to be coated with
anything.

cheers,
Rosemary


Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
E rosemary.white-at-csiro.au

On 1/02/11 11:29 PM, "stefan.diller-at-t-online.de" {stefan.diller-at-t-online.de}
wrote:

}
}
}
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} Dear All,
} I would like to do some work on lichen. Now I got access to a huge herbarium
} collection of lichen.
} What it do any good to use these room-dried specimen, moisture them, fix and
} go through ethanol to critcal point drying?
} Any experience with this out there?
}
} Best wishes,
} Stefan



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From: naomi_mccallum-at-health.qld.gov.au
Date: Tue, 1 Feb 2011 21:00:53 -0600
Subject: [Microscopy] KOS EM (Milestone) Microwave Tissue Processor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All

We have been offered a demonstration in our laboratory of the Milestone "KOS EM" tissue processor. Has anyone encountered this product before? Perhaps some of you have had experience with other Microwave tissue processors that you may wish to share.

Thanks in advance
Naomi

Naomi McCallum
Supervising Scientist, EM Unit, Anatomical Pathology
Pathology Queensland
_________________________________________________
Clinical and Statewide Services Division| Queensland Health

Block 7 Level 2
RBWH Queensland 4029
Ph: 07 3636 8057
Mob:
Fax: 07 3636 8908
Email: naomi_mccallum-at-health.qld.gov.au
Web: http://www.health.qld.gov.au/qhcss/qhps



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From: donc-at-asmicro.com
Date: Tue, 1 Feb 2011 21:16:05 -0600
Subject: [Microscopy] Re: [a] STM tip advice. - Commercial reply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Justin,
We can supply small quantities of Pt-Ir STM tips at a price much lower than
"$200 per tip". The tips we have were manufactured at Digital Instruments
for use with their STM. Please contact me offline and share details of the
wire size requirements of the Easyscan stm.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc. E-Mail: donc-at-asmicro.com
3250 N. Post Rd., Ste. 120 Voice: 317-895-5630
INDIANAPOLIS IN 46226 USA Toll free: 800-374-8557 (in USA & Canada)
web: http://www.asmicro.com Fax: 317-895-5652
[business activities: analytical services in AFM, AFM probes, consulting,
training,
calibration and test specimens, calibration and measurement software,
used NanoScope equipment.] 2009-2010 is our 20th year in business!
=============================================
----- Original Message -----
From: kraftpiano-at-gmail.com
To: donc-at-asmicro.com
Sent: Friday, January 21, 2011 12:10 AM
Subject: [a] [Microscopy] STM tip advice.





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I know that I usually talk about SEM applications, but recently I have
been given the task of setting up and playing with a scanning tunneling
microscope. We have a small length of platinum/iridium wire, and the
instructions tell us to take a pair of cutters and gently squeeze and pull
the wire to make an atomic scale tip. So far, we have been unsuccessful at
producing a tip that functions properly in the machine (It's a Easyscan
Nanosurf).

I found some documentation online about etching tungsten tips in KOH, so I
sacrificed an SEM filament and tried it- at first it looked good, but the
tip still ended up etched entirely flat at the surface of the KOH solution.

The purpose of my playing with this is to try to develop a set of
instructions that anyone (Physics undergrads- most of whom are theorists and
think less of us experimentalists) can use to get this up and running for
one of our lab courses.

Does anyone have any suggestions? Since this is a lab course, there is no
budget to be spending $200 per tip on tips that will just end up smashed
into the surface of a specimen anyway.

Thanks,

Justin A. Kraft

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From: terry.cooper-at-btinternet.com
Date: Wed, 2 Feb 2011 05:39:05 -0600
Subject: [Microscopy] Ultramicrotome history

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers

Sorry for the delay in response to this topic, but due to an error on my on
my part my previous communication went astray. For those who may be
interested in the history of ultramicrotomy I do have a copy of an article
in PDF form which traces the development of the ultramicrotome from "wedge"
sections in the late thirties where the thin end of the wedge was
(hopefully) transparent to electrons, through the high speed era (actually
up to 57000 rpm), overcoming embedding limitations and finally discussing
the first generation of commercial instruments.

Please contact me on terry.cooper-at-btinternet.com and I can attach the
missive,

Best regards

Terry Cooper
TAAB Laboratories Equipment
3 Minerva House, Calleva Park
Aldermaston, Berks, RG7 8NA, England
Tel ++44(0)118 981 7775
Fax ++44(0)118 981 7881
e-mail sales-at-taab.co.uk
www.taab.co.uk


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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 2 Feb 2011 07:55:51 -0600
Subject: [Microscopy] viaWWW:SEM sample storing

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Email: wadowska-at-upei.ca Name: Dorota Wadowska

Organization: Atlantic Veterinary College at UPEI

Title-Subject: [Filtered] SEM sample storing

Message: Hello all,
One of our EM lab users asked me a question: what is the best way to
store SEM samples? I know you'll all say that the best is to process and
coat first. He wants to store them in 2% glutaraldehyde and process
later since he does not have time at the moment. What is your
experience? I am not an expert in SEM.
Thank you Dorota
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From: waynezhao.microscopy-at-gmail.com
Date: Wed, 2 Feb 2011 08:50:25 -0600
Subject: [Microscopy] PFA Engineer & Technicians openings at Global Foundries

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dorota
To my experience specimens can stay in glutar for an indefinite time. I have
processed samples after } 3 years stored in glutar inside the fridge, and
they looked same as others being processed within a few days. In rare cases
I've seen mushrooms developing in samples that stayed only a few weeks in
glutar. There I suspect glutar was old, samples were dirty from the
beginning, or some other unedintified factor was present. If you process and
coat the specimens I think it is better to view them soon, otherwise can get
humidified and morphology deteriorates.
In conclusion I think it is not a bad idea to have your samples staying in
glutar until you have the time to view them.
With best wishes
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
eikonika-at-otenet.gr
www.aim.cat
***********************************



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Dear Colleagues,

Openings for Engineers & Technicians in Physical Failure Analysis (PFA) are
available in Global Foundries, R&D Center at East Fishkill, NY.

Engineers should be strong in FIB & SEM / basic TEM & device physics.
Industry experience with device / wafer-fab helpful.

Technicians should have extensive hands-on skills in various TEM sample-prep
techniques, e.g., delayering / mechanical polishing / FIB / CLM, etc.
Previous experience at high-throughput environments preferred, but not
a requirement.

Applicants should demonstrate unrestricted employment eligibility in USA,
which usually requires US citizenship or green card.

If interested, please send a cover letter and resume, "offline", to
wayne.zhao-at-globalfoundries.com .

Wayne

-------------------------------------------
Wayne Zhao, PhD
Technical Lead of PFA Team
Sr. Member of Technical Staff
R & D Center at East Fishkill, NY
Global Foundries
-------------------------------------------

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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 2 Feb 2011 08:59:53 -0600
Subject: [Microscopy] viaWWW:HMDS, How does it work...

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Email: bbandli-at-d.umn.edu Name: Bryan Bandli

Organization: University of Minnesota, Duluth

Title-Subject: [Filtered] HMDS, How does it work...

Message: Hi All,

I'm looking for a reference to explain just how/why HMDS works to dry
specimens for SEM observation. There are plenty of references showing
that it does, in fact, work and produces good results in many cases but
I have yet to find an explanation as to the "why".
Is it that HMDS has the right physical properties (surface tension) to
evaporate without causing drying artifacts?

Or, is there something about the silanization of the sample that helps
make it more robust, or both, or am I completely lost (quite likely)?

Thanks in advance for your input!

Cheers,

Bryan Bandli
University of MN, Duluth

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From: kenconverse-at-qualityimages.biz
Date: Wed, 2 Feb 2011 09:29:21 -0600
Subject: [Microscopy] viaWWW:HMDS, How does it work...

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Bryan,
I believe it has to do with very low surface tension. It also doesn't work
well across the board. I understand that it works well with insects, but
one of my sons did a science fair project comparing dried, freeze dried and
HMDS treated green pepper. In his case the HMDS didn't appear to be any
better than drying. His best results were from plunging small pieces into
acetone cooled with a Peltier cooler, then transferring to another Peltier
cooler in a vacuum evaporator until dehydrated. He was not able to compare
it to CPD prep as we didn't have the requisite CO2. Obviously, the plunge
freezing was not done at typical temperatures, so there was probably ice
damage in those samples, but they looked the best of the bunch.

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
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kenconverse-at-qualityimages.biz
qualityimages.biz


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Email: bbandli-at-d.umn.edu Name: Bryan Bandli

Organization: University of Minnesota, Duluth

Title-Subject: [Filtered] HMDS, How does it work...

Message: Hi All,

I'm looking for a reference to explain just how/why HMDS works to dry
specimens for SEM observation. There are plenty of references showing
that it does, in fact, work and produces good results in many cases but
I have yet to find an explanation as to the "why".
Is it that HMDS has the right physical properties (surface tension) to
evaporate without causing drying artifacts?

Or, is there something about the silanization of the sample that helps
make it more robust, or both, or am I completely lost (quite likely)?

Thanks in advance for your input!

Cheers,

Bryan Bandli
University of MN, Duluth

Login Host: 131.212.37.204
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From: dsherman-at-purdue.edu
Date: Wed, 2 Feb 2011 10:37:12 -0600
Subject: [Microscopy] Re: Ultramicrotome history

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Although I have HMDS and have used it, I'm no expert.

That said,

given the following pieces, I'd say the lower surface tension allows better
permeation and the evaporation rate is near sublimatic in process because of
both the lower surface tension and higher vapor pressure [the lower surface
tension maximizes the area and the thinness of the film, both of which
increase evaporation flux]:

1. Bray, Comparison of Hexamethyldisilazane (HMDS), Peldri 11, and
Critical-Point Drying Methods for SEM of Biological Specimens, Microsc Res
Tech, 26, 489-495, 1993.

"Recently, several drying methods which claim to alleviate
some of the disadvantages of CPD have been
described. One of these methods (Kennedy et al., 1989)
is similar to freeze drying and utilizes a sublimation
dehydrant, Peldri 11, which is frozen and then sublimed
from specimens. The Peldri 11 procedure requires no
special equipment but can take several hours to perform
a run. Other methods involve the use of low-surface-
tension solvents such as Hexamethyldisilazane
(HMDS) (Nation, 1983), tetramethylsilane (TMS) (Dey
et al., 19891, and dimethoxypropane (DMP) (Weyda,
1992). These solvents evaporate directly from tissue in
minutes, and infiltration times usually take { 1 hr."

2. Beno, Processing of soft pupae and uneclosed pharate adults of
Drosophila for scanning electron microscopy, Microsc Res Tech, 70,
1022-1027, 2007

"We found that it is crucial to leave minute amount of above
used solvents (ethanols, acetone, and mixture of acetone:
hexamethyldisilazane) in eppendorf tube prior to each
exchange to prevent animal from fast drying and damage."



Tony

......................................................................
Andrew Anthony "Tony" Havics, CHMM, CIH, PE
pH2, LLC 5250 E US 36, Suite 830 Avon IN 46123
www.ph2llc.com
(317) 718-7020 off
(317) 718-7038 fax
(317) 409-3238 cell

90% of Risk Management is knowing where to place the decimal point...any
consultant can give you the other 10%(SM)

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-----Original Message-----
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To: ph2-at-sprynet.com

Fascinating article. Some of my recollections (possibly with some
inaccuracies but the best I can recollect) involve Fernandez-Moran who is
credited with developing the first diamond knives for use with
ultra-microtomes as well as a cryo ultra-microtome and a number of other
interesting developments.

I worked for Fernandez-Moran at the U. of Chicago for a few years starting
in 1963. This was after he was forced to leave Venezuela and then did a
short stay at MIT before being recruited by U. of C. He had a microtome,
presumably of his design, that only he used on rare occasion. Almost all
the imaging done in the lab was with negatively stained samples.

He, of course, had his diamond knives. The technique to make them was
perfected at the lab for neurological research that he built in Venezuela.
(He was forced to leave Venezuela when the government was taken over by a
military coup and he was on the wrong side, but that's another story.) He
had a workshop there with his diamond cutters, etc. After developing the
knives, he sent them out to the leading investigators of the day to get them
to try them so that they would then buy them. Dupont picked up on the idea
and also began making them for sale. Moran had patented the process so was
able to sue Dupont and did win. I believe he later agreed to give them
rights to make and market the knives.

Moran's lab at Chicago was quite a place. It was a semi-clean room lab in
the basement of the Research Institute. The floors were raised so that all
the water and vacuum lines for the microscopes were underneath with
mechanical pumps and water recirculators a long ways away from the
microscopes. He had 3 Seimens 1 and 1A TEMS on vibration mounts with the
raised floor cut around the microscope bases so that moving a chair would
not affect the TEM stability. A motor generator located in the attic of the
building provided stable power. I started out as a technician and we all
wore white nylon lab coats, white rubber shoes that we washed weekly, and
little white hats. Visitors suited up in lab coats with plastic bags over
their shoes. Pre-pumps to evacuate the film cameras were located two floors
up. We would put on our red goggles to retain our dark adaptation, put
plastic bags over our plastic shoes, and shuffle up to get new film
cameras...looked like Martians!

It was an interesting time. There was also a couple of Japanese scientists
working on an early Hitachi microscope trying to set resolution records.
They would literally disassemble the microscope after just a few tries to
clean it and get ready for the next attempts. They were the only ones with
the patience to deal with that microscope. Later Perk and Elmer Company
came along and helped with design changes to make it more user-friendly and
thus more marketable.

Pointed filaments were also made in the lab. E.F. Fullam came one time to
see how they were made and then was able to start selling them commercially.

Moran built a helium-cooled microscope to improve resolution using
superconducting lenses. I do not know if it was originally his idea or if he
"borrowed" someone else's idea. However, they built a liquid helium
recirculator system, again with most of it in the attic 4 stories up. The
helium flowed through a special jacket on the microscope that encased the
entire upper part of the column through the objective lens. They managed to
get a few images but then Moran lost interest. A series of health problems
shortly thereafter led to closing down the lab and and end to a very
interesting few years. Louie Ouwerkerk was a Dutch engineer who, along with
the great U. of Chicago Instrument shop, designed and built a lot of Moran's
ideas.

Debby

P.S. I have time to write this as the university is closed due to a major
snow storm that hit us over the last 24 hours. Looks very pretty out there
but....

---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy




On 2/2/11 6:43 AM, "terry.cooper-at-btinternet.com"
{terry.cooper-at-btinternet.com} wrote:

}
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} Dear Listers
}
} Sorry for the delay in response to this topic, but due to an error on my on
} my part my previous communication went astray. For those who may be
} interested in the history of ultramicrotomy I do have a copy of an article
} in PDF form which traces the development of the ultramicrotome from "wedge"
} sections in the late thirties where the thin end of the wedge was
} (hopefully) transparent to electrons, through the high speed era (actually
} up to 57000 rpm), overcoming embedding limitations and finally discussing
} the first generation of commercial instruments.
}
} Please contact me on terry.cooper-at-btinternet.com and I can attach the
} missive,
}
} Best regards
}
} Terry Cooper
} TAAB Laboratories Equipment
} 3 Minerva House, Calleva Park
} Aldermaston, Berks, RG7 8NA, England
} Tel ++44(0)118 981 7775
} Fax ++44(0)118 981 7881
} e-mail sales-at-taab.co.uk
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}
}
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==============================Original Headers==============================
17, 31 -- From dsherman-at-purdue.edu Wed Feb 2 10:37:11 2011
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17, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
17, 31 -- To: "terry.cooper-at-btinternet.com" {terry.cooper-at-btinternet.com} ,
17, 31 -- "message to:
17, 31 -- MSA list" {microscopy-at-microscopy.com}
17, 31 -- Date: Wed, 2 Feb 2011 11:37:05 -0500
17, 31 -- Subject: Re: [Microscopy] Ultramicrotome history
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From: steven.samuelsson-at-sri.com
Date: Wed, 2 Feb 2011 11:16:37 -0600
Subject: [Microscopy] Cryostat Knife Holder Needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listserve:

We recently acquired a vintage Reichert- Jung, Cryocut 1800.
Unfortunately, it is missing much of the knife holder assemble. Does
anyone on the list have these parts and are willing to sell or surplus
them? The unit is in good condition and should serve us well.

Thanks so much.

Steve

--
Steven Samuelsson, PhD
Director
Cell and Molecular Imaging Facility
100-51
SRI International, Inc.
333 Ravenswood Ave
Menlo Park, CA 94061
(650) 859-2980


==============================Original Headers==============================
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6, 18 -- Subject: Cryostat Knife Holder Needed
==============================End of - Headers==============================




From: NEERAJG-at-clemson.edu
Date: Wed, 2 Feb 2011 13:27:10 -0600
Subject: [Microscopy] Re: Ultramicrotome history

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Great Story Debby, Thanks for sharing it with the list.

Stay Warm!

Best,

Neeraj.



-----Original Message-----
X-from: dsherman-at-purdue.edu [mailto:dsherman-at-purdue.edu]
Sent: Wednesday, February 02, 2011 11:46 AM
To: Neeraj Gohad

Fascinating article. Some of my recollections (possibly with some
inaccuracies but the best I can recollect) involve Fernandez-Moran who is
credited with developing the first diamond knives for use with
ultra-microtomes as well as a cryo ultra-microtome and a number of other
interesting developments.

I worked for Fernandez-Moran at the U. of Chicago for a few years starting
in 1963. This was after he was forced to leave Venezuela and then did a
short stay at MIT before being recruited by U. of C. He had a microtome,
presumably of his design, that only he used on rare occasion. Almost all
the imaging done in the lab was with negatively stained samples.

He, of course, had his diamond knives. The technique to make them was
perfected at the lab for neurological research that he built in Venezuela.
(He was forced to leave Venezuela when the government was taken over by a
military coup and he was on the wrong side, but that's another story.) He
had a workshop there with his diamond cutters, etc. After developing the
knives, he sent them out to the leading investigators of the day to get them
to try them so that they would then buy them. Dupont picked up on the idea
and also began making them for sale. Moran had patented the process so was
able to sue Dupont and did win. I believe he later agreed to give them
rights to make and market the knives.

Moran's lab at Chicago was quite a place. It was a semi-clean room lab in
the basement of the Research Institute. The floors were raised so that all
the water and vacuum lines for the microscopes were underneath with
mechanical pumps and water recirculators a long ways away from the
microscopes. He had 3 Seimens 1 and 1A TEMS on vibration mounts with the
raised floor cut around the microscope bases so that moving a chair would
not affect the TEM stability. A motor generator located in the attic of the
building provided stable power. I started out as a technician and we all
wore white nylon lab coats, white rubber shoes that we washed weekly, and
little white hats. Visitors suited up in lab coats with plastic bags over
their shoes. Pre-pumps to evacuate the film cameras were located two floors
up. We would put on our red goggles to retain our dark adaptation, put
plastic bags over our plastic shoes, and shuffle up to get new film
cameras...looked like Martians!

It was an interesting time. There was also a couple of Japanese scientists
working on an early Hitachi microscope trying to set resolution records.
They would literally disassemble the microscope after just a few tries to
clean it and get ready for the next attempts. They were the only ones with
the patience to deal with that microscope. Later Perk and Elmer Company
came along and helped with design changes to make it more user-friendly and
thus more marketable.

Pointed filaments were also made in the lab. E.F. Fullam came one time to
see how they were made and then was able to start selling them commercially.

Moran built a helium-cooled microscope to improve resolution using
superconducting lenses. I do not know if it was originally his idea or if he
"borrowed" someone else's idea. However, they built a liquid helium
recirculator system, again with most of it in the attic 4 stories up. The
helium flowed through a special jacket on the microscope that encased the
entire upper part of the column through the objective lens. They managed to
get a few images but then Moran lost interest. A series of health problems
shortly thereafter led to closing down the lab and and end to a very
interesting few years. Louie Ouwerkerk was a Dutch engineer who, along with
the great U. of Chicago Instrument shop, designed and built a lot of Moran's
ideas.

Debby

P.S. I have time to write this as the university is closed due to a major
snow storm that hit us over the last 24 hours. Looks very pretty out there
but....

---
Debby Sherman, Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy




On 2/2/11 6:43 AM, "terry.cooper-at-btinternet.com"
{terry.cooper-at-btinternet.com} wrote:

}
}
}
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Dear Listers
}
} Sorry for the delay in response to this topic, but due to an error on my on
} my part my previous communication went astray. For those who may be
} interested in the history of ultramicrotomy I do have a copy of an article
} in PDF form which traces the development of the ultramicrotome from "wedge"
} sections in the late thirties where the thin end of the wedge was
} (hopefully) transparent to electrons, through the high speed era (actually
} up to 57000 rpm), overcoming embedding limitations and finally discussing
} the first generation of commercial instruments.
}
} Please contact me on terry.cooper-at-btinternet.com and I can attach the
} missive,
}
} Best regards
}
} Terry Cooper
} TAAB Laboratories Equipment
} 3 Minerva House, Calleva Park
} Aldermaston, Berks, RG7 8NA, England
} Tel ++44(0)118 981 7775
} Fax ++44(0)118 981 7881
} e-mail sales-at-taab.co.uk
} www.taab.co.uk
}
}
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==============================Original Headers==============================
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17, 31 -- From: "Sherman, Debra M" {dsherman-at-purdue.edu}
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17, 31 -- Date: Wed, 2 Feb 2011 11:37:05 -0500
17, 31 -- Subject: Re: [Microscopy] Ultramicrotome history
17, 31 -- Thread-Topic: [Microscopy] Ultramicrotome history
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29, 37 -- From NEERAJG-at-clemson.edu Wed Feb