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From: microscopylistserver-noreply-at-microscopy.com
Date: Sun, 1 Jan 2012 08:03:02 -0600
Subject: [Microscopy] Administrivia: Happy New Year - 2012

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the 20th year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2011, the ListServer delivered 2471 messages to over 3300 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated 275+ Gb of Email traffic and over
8.1 Million Email messages were sent out this year by my tired little server.
As usual you don't want to know how much Junk Mail and spam has been filtered out.


The complete Microscopy ListServer Archives for 2011-1993 (~ are on-line at

http://www.microscopy.com.

A couple of IMPORTANT reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
your Email address from the listserver. The out-of-office / on-vacation
autoreply messages are a real nuisance to posters.

Do not reply to message with the return address of:

MicroscopyListserver-noreply-at-microscopy.com

these are messages forwarded usually from the WWW posting form. They do not
go back to the poster but rather into a black hole, which I rarely check.
If you see a message that has this "No-Reply" return address please post your
reply/comment/answer to:

Microscopy-at-microscopy.com

or if you wish to reply privately, look at the username in the body of
the message the originators Email address is usually listed therein.

As always if you have questions about suitability of postings or
are having problems, feel free to contact me at (zaluzec-at-microscopy.com)

Cheers,

Nestor
Your Friendly Neighborhood SysOp

==============================Original Headers==============================
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From: microscopylistserver-noreply-at-microscopy.com
Date: Sun, 1 Jan 2012 10:51:40 -0600
Subject: [Microscopy] viaWWW:process SEM images by adobe photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
isha.mutreja-at-gmail.com as well as the MIcroscopy Listserver
---------------------------------------------------------------------------

Email: isha.mutreja-at-gmail.com
Name: isha mutreja

Organization: Ulster University

Title-Subject: [Filtered] Biomedical Sciences

Message: How to process SEM images by adobe photoshop to add coloured effects to those images. Thank
you in anticipation. Looking forward for some help and guidance.

Login Host: 2.220.237.78
---------------------------------------------------------------------------



==============================Original Headers==============================
8, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan 1 10:51:40 2012
8, 25 -- Received: from znl.com ([206.69.208.20])
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8, 25 -- From: MicroscopyListserver-NoReply {microscopylistserver-noreply-at-microscopy.com}
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8, 25 -- MIME-Version: 1.0
8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
8, 25 -- Subject: viaWWW:process SEM images by adobe photoshop
8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
8, 25 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: d.sokolov-at-massey.ac.nz
Date: Sun, 1 Jan 2012 21:10:42 -0600
Subject: [Microscopy] Re: viaWWW:process SEM images by adobe photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Isha,

here is the permalink to MIAWiki page on the topic:
http://confocal-manawatu.pbworks.com/w/page/44308564/Color%20SEM%20Images

Hope that will be helpful.

Cheers,
Dmitry
MIAWiki for Mass Collaboration

On Mon, January 2, 2012 5:57 am,
microscopylistserver-noreply-at-microscopy.com wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying please copy both
} isha.mutreja-at-gmail.com as well as the MIcroscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: isha.mutreja-at-gmail.com
} Name: isha mutreja
}
} Organization: Ulster University
}
} Title-Subject: [Filtered] Biomedical Sciences
}
} Message: How to process SEM images by adobe photoshop to add coloured
} effects to those images. Thank
} you in anticipation. Looking forward for some help and guidance.
}
} Login Host: 2.220.237.78
} ---------------------------------------------------------------------------
}
}
}
} ==============================Original
} Headers==============================
} 8, 25 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan 1
} 10:51:40 2012
} 8, 25 -- Received: from znl.com ([206.69.208.20])
} 8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} q01Gpe5S004142
} 8, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 10:51:40 -0600
} 8, 25 -- Received: from localhost (localhost [127.0.0.1])
} 8, 25 -- by znl.com (Postfix) with ESMTP id 5282B46A6EE
} 8, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 10:51:40 -0600
} (CST)
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} 8, 25 -- Received: from znl.com ([127.0.0.1])
} 8, 25 -- by localhost (server.microscopy.com [127.0.0.1]) (amavisd-new,
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} 8, 25 -- with ESMTP id 2hp0K5OGNV-K for {microscopy-at-microscopy.com} ;
} 8, 25 -- Sun, 1 Jan 2012 10:51:39 -0600 (CST)
} 8, 25 -- Received: from Nestor-J-Zaluzecs-Mac-Pro.local (mac22.zaluzec.com
} [206.69.208.22])
} 8, 25 -- by znl.com (Postfix) with ESMTPA id 336FC46A6E3
} 8, 25 -- for {microscopy-at-microscopy.com} ; Sun, 1 Jan 2012 10:51:39 -0600
} (CST)
} 8, 25 -- Message-ID: {4F008F1A.1090502-at-microscopy.com}
} 8, 25 -- Date: Sun, 01 Jan 2012 10:51:38 -0600
} 8, 25 -- From: MicroscopyListserver-NoReply
} {microscopylistserver-noreply-at-microscopy.com}
} 8, 25 -- Reply-To: isha.mutreja-at-gmail.com
} 8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.6;
} en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
} 8, 25 -- MIME-Version: 1.0
} 8, 25 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
} 8, 25 -- Subject: viaWWW:process SEM images by adobe photoshop
} 8, 25 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
} 8, 25 -- Content-Transfer-Encoding: 7bit
} ==============================End of -
} Headers==============================
}


--
Dr. Dmitry Sokolov
Institute of Fundamental Sciences
Massey University, Palmerston North
New Zealand


==============================Original Headers==============================
8, 27 -- From d.sokolov-at-massey.ac.nz Sun Jan 1 21:10:42 2012
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8, 27 -- Date: Mon, 2 Jan 2012 16:10:37 +1300 (NZDT)
8, 27 -- Subject: Re: [Microscopy] viaWWW:process SEM images by adobe photoshop
8, 27 -- From: "Dmitry Sokolov" {d.sokolov-at-massey.ac.nz}
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From: milesd-at-us.ibm.com
Date: Tue, 3 Jan 2012 10:33:38 -0600
Subject: [Microscopy] Re: Administrivia: Happy New Year - 2012

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Nestor,

A very Happy New Year to you, too! I would like to thank you for the
Fantastic job you do with the listserver. It has been a very positive
influence in many lives, and careers.

Best Regards,
Darrell Miles



microscopylistserver-noreply-at-microscopy.com wrote on 01/01/2012 09:03:45
AM:

--| [image removed]
--|
--| [Microscopy] Administrivia: Happy New Year - 2012
--|
--| microscopylistserver-noreply
--|
--| to:
--|
--| Darrell Miles
--|
--| 01/01/2012 09:04 AM
--|
--| Please respond to microscopylistserver-noreply
--|
--|
--|
--|
--|
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--| Happy New Year Colleagues;
--|
--| Welcome to the 20th year of operation of the Microscopy ListServer
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--|
--| During 2011, the ListServer delivered 2471 messages to over 3300
--| subscribers
--| around the world, with minimal hassels (that I know about). For
--| those of you that are
--| statistics junkies this year you generated 275+ Gb of Email traffic
and
over
--| 8.1 Million Email messages were sent out this year by my tired little
server.
--| As usual you don't want to know how much Junk Mail and spam has been
--| filtered out.
--|
--|
--| The complete Microscopy ListServer Archives for 2011-1993 (~ are
on-line
at
--|
--| http://www.microscopy.com.
--|
--| A couple of IMPORTANT reminders:
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--| your Email address from the listserver. The out-of-office /
on-vacation
--| autoreply messages are a real nuisance to posters.
--|
--| Do not reply to message with the return address of:
--|
--| MicroscopyListserver-noreply-at-microscopy.com
--|
--| these are messages forwarded usually from the WWW posting form. They
do
not
--| go back to the poster but rather into a black hole, which I rarely
check.
--| If you see a message that has this "No-Reply" return address please
post
your
--| reply/comment/answer to:
--|
--| Microscopy-at-microscopy.com
--|
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--|
--| As always if you have questions about suitability of postings or
--| are having problems, feel free to contact me at
(zaluzec-at-microscopy.com)
--|
--| Cheers,
--|
--| Nestor
--| Your Friendly Neighborhood SysOp
--|
--| ==============================Original
Headers==============================
--| 15, 24 -- From microscopylistserver-noreply-at-microscopy.com Sun Jan
--| 1 08:03:02 2012
--| 15, 24 -- Received: from znl.com ([206.69.208.20])
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-0600
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--| 15, 24 -- MIME-Version: 1.0
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--| 15, 24 -- Subject: Administrivia: Happy New Year - 2012
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--=_alternative 005932248525797A_=
Content-Type: text/html; charset="US-ASCII"


|--br--||--font size=2 face="sans-serif"--|Dear Nestor,|--/font--|
|--br--|
|--br--||--font size=2 face="sans-serif"--|A very Happy New Year to you,
too!  I
would like to thank you for the Fantastic job you do with the listserver.
 It has been a very positive influence in many lives, and
careers.|--/font--|
|--br--|
|--br--||--font size=2 face="sans-serif"--|Best Regards,|--/font--|
|--br--||--font size=2 face="sans-serif"--|Darrell Miles|--/font--|
|--br--|
|--br--|
|--br--|
|--br--||--tt--||--font
size=2--|microscopylistserver-noreply-at-microscopy.com wrote
on 01/01/2012 09:03:45 AM:|--br--|
|--br--|
> [image removed] |--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> [Microscopy] Administrivia: Happy New Year - 2012|--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> microscopylistserver-noreply |--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> to:|--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> Darrell Miles|--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> 01/01/2012 09:04 AM|--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> Please respond to microscopylistserver-noreply|--/font--||--/tt--|
|--br--||--tt--||--font size=2--|> |--br--|
> |--br--|
> |--br--|
> |--br--|
>
----------------------------------------------------------------------------|--br--|
> The Microscopy ListServer -- CoSponsor:  The Microscopy Society
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>
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> |--br--|
> Happy New Year Colleagues;|--br--|
> |--br--|
> Welcome to the 20th  year of operation of the Microscopy
ListServer|--br--|
> a free service to the world wide microscopy community,
sponsored|--br--|
> jointly by your Friendly Neighborhood SysOp and the Microscopy
Society|--br--|
> of America.|--br--|
> |--br--|
> During 2011, the   ListServer delivered 2471  messages
 to
over 3300|--br--|
> subscribers|--br--|
> around the world, with  minimal hassels (that I  know
about).
For |--br--|
> those of you that are|--br--|
> statistics junkies this year you generated  275+ Gb of Email
traffic and over|--br--|
> 8.1 Million Email messages were sent out this year by my tired little
server.|--br--|
> As usual you don't want to know how much Junk Mail and spam has
been|--br--|
> filtered out.|--br--|
> |--br--|
> |--br--|
> The complete Microscopy ListServer Archives for 2011-1993 (~ are
 on-line
 at|--br--|
> |--br--|
>      |--/font--||--/tt--||--a
href=http://www.microscopy.com/--||--tt--||--font
size=2--|http://www.microscopy.com|--/font--||--/tt--||--/a--||--tt--||--font
size=2--|.|--br--|
> |--br--|
> A couple of IMPORTANT reminders:|--br--|
> |--br--|
> If you leave on vacation/holiday  use the on-line form to
UNSUBSCRIBE|--br--|
> your Email address from the listserver.   The out-of-office /
on-vacation|--br--|
> autoreply messages are a real nuisance to posters.|--br--|
> |--br--|
> Do not reply to message with the return address of:|--br--|
> |--br--|
>    MicroscopyListserver-noreply-at-microscopy.com|--br--|
> |--br--|
> these are messages forwarded usually from the WWW posting form. They
do not|--br--|
> go back to the poster but rather into a black hole, which I rarely
check.|--br--|
> If you see a message that has this "No-Reply" return
address
please post your|--br--|
> reply/comment/answer to:|--br--|
>      |--br--|
>    Microscopy-at-microscopy.com|--br--|
> |--br--|
> or if you wish to reply privately, look at the username in the body
of|--br--|
> the message the originators Email address is usually listed
therein.|--br--|
> |--br--|
> As always if you have questions about suitability of postings
or|--br--|
> are having problems, feel free to contact me at
(zaluzec-at-microscopy.com)|--br--|
> |--br--|
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 4 Jan 2012 07:28:26 -0600
Subject: [Microscopy] viaWWW:Assistant professor position at ETH Zurich in electron microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

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Email: ban-at-mol.biol.ethz.ch Name: Nenad Ban

Organization: ETH Zurich

Title-Subject: [Filtered] Assistant professor position at ETH Zurich in electron microscopy

Message: Dear Colleagues, I would like to draw your attention to an assistant professor position in
Electron Microscopy / Structural Biology at the ETH Zurich in Switzerland. The text below describes
the position that has been advertised in Nature and Science.
Details of the position are negotiable depending on the qualifications of the candidate. We
encourage excellent candidates to apply, for more information you may contact Prof. Nenad Ban,
address provided below.

With best wishes, Nenad Ban

==============================Original Headers==============================
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 4 Jan 2012 19:51:45 -0600
Subject: [Microscopy] viaWWW:Intergating Prior Stage with BioRad 2100 CLSM

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Email: j.knowles-at-ucl.ac.uk Name: Jonathan Knowles

Organization: UCL

Title-Subject: [Filtered] Intergating Prior Stage with BioRad 2100 CLSM

Message: Hi

We have a Prior Stsge and BioRad 2100. I was looking to configure the stage within the LAsersharp
software. The manual points to a cfg file, but I am unsure what to change/configure. Can anyone
provide some guidance?

Thanks

Jonathan

Login Host: 144.82.51.63
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From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 4 Jan 2012 19:58:54 -0600
Subject: [Microscopy] Diposal Advice Needed for Ammonium Molybdate Solution

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

I have approximatley 200 ml of 80 mM Ammonium Molybdate
solution left by a user in our Lab. The solution was used
as a stain for biological specimens. Can anyone familiar
with this stain point me to information concerning proper
method of disposal.

The MSDS sheet on Ammonium Molybdate indicates care should
be taken.


Thanks...

Nestor
Your Friendly Neighborhood SysOp.



--
===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Electron Microscopy Center
Materials Science/Bldg 212
Argonne, Illinois 60439 USA

Tel: 1-530-NES-TORZ (1-530-637-8679)
Fax: 1-630-252-4798

Email: Zaluzec-at-aaem.amc.anl.gov

iChat:Zaluzec-at-AIM
Skype: Zaluzec
TPM: http://tpm.amc.anl.gov
WWW: http://www.amc.anl.gov

Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
E.P. Wigner Fellow - Oak Ridge National Laboratory
Senior Fellow of the Computational Institute - University of Chicago
Past President Microscopy Society of America

===========================================

The box said ...
"This program requires Win 95/98/NT/2K/XP/Vista or better..."
So I bought a Mac !

===========================================

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From: marisa_ote-at-yahoo.com
Date: Thu, 5 Jan 2012 07:53:54 -0600
Subject: [Microscopy] postdoctoral position available on cryo-EM and electron tomography

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A postdoctoral position is available at the University of Wisconsin-Madison to work on electron tomography and cryo-electron microscopy of membrane deformation mechanisms mediated by ESCRT proteins. The postdoctoral researcher will work as part of a multidisciplinary team integrated by cell biologists, biophysicists, and virologists to understand structural aspects of ESCRT-mediated membrane budding in multiple organisms/systems, including plants, worms, and mammalian cultured cells.

The project entails imaging of plastic and vitreous sections of plant and animal tissues by electron tomography.

Applicants must have a Ph.D. and proven experience in the field of electron microscopy, electron tomography, and cryo-electron microscopy. Experience with vitreous sectioning and single particle analysis is desirable. Excellent written and oral skills and the ability to work collaboratively with others are required.

For more information of ongoing research in the labs involved in this project, please visit the following websites:
http://www.botany.wisc.edu/otegui/welcome.html
http://www.bmolchem.wisc.edu/faculty/audhya.html
http://www.mcardle.wisc.edu/faculty/bio/ahlquist_p.html

Interested candidates should submit a letter stating experience, and also include a CV and the names and contact information (phone and e-mail) of individuals who can provide a professional letter of references in a single pdf file to Dr Marisa Otegui (otegui-at-wisc.edu).

Marisa Otegui
Associate Professor
B119 Birge Hall
430 Lincoln Drive
Department of Botany
Madison, WI 53706
Lab webpage http://www.botany.wisc.edu/otegui/welcome.html

Phone: (608)265-5703
Fax: (608)262-7509


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From: kjmorris-at-well.ox.ac.uk
Date: Thu, 5 Jan 2012 09:23:20 -0600
Subject: [Microscopy] viaWWW:process SEM images by adobe photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Isha,

As well as Dmitry's www5.pbrc.hawaii.edu/microangela etc links, below is
some info copy and pasted from a very similar discussion on colouring B&W
TEM images back in January 2010 on the microscopy list-server [to find all
these posts, the threads were under the title: 'Image Colorizing']:


I tried out colorizing an SEM image with Photoshop CS4 and the results
seemed fine:

Original SEM image of pollen
http://en.wikipedia.org/wiki/File:Misc_pollen.jpg

I Colorized the image using the method in my last post, see below [you could
use Photoshop CS4 or Elements 8]
http://www.well.ox.ac.uk/cytogenetics/pollen/pollen_colorized.jpg
It took well under an hour to select and colorize the whole image [and I
found it quite therapeutic].

I also applied a Pseudocolor look-up table [LUT] applied using Photoshop CS4
[and Elements 8 has similar tools]
http://www.well.ox.ac.uk/cytogenetics/pollen/pollen_pseudo.jpg
For details of creating pseudocolor [false colour] with LUTs [e.g. Image,
Mode, Color table] with Photoshop CS4 see the likes of
http://www.imagingandanalysis.com/pseudo.pdf
Adobe's help, and search the internet.

----------------------------------------------------------------------------
----------------------------------------------------------------------------
------------------
Details of the colouring technique in Photoshop CS4:

Hi Isha,

It is quite easy, if time consuming, to do this Photoshop CS4 or CS5 [CS5
Extended's only £130 with Educational discounts - on a really tight budget
the likes Serif PhotoPlus X4 or Adobe Elements 10 will suffice].

There's always different ways to approach something like this in Photoshop
CS5 [or earlier versions], but I'd try:

Load the SEM Photo and manually select the region you wish to colourize,
using say the 'quick selection tool' - and you can add/reject [shift/alt]
bits of selected regions with a lasso tool. Then ensure the image is
converted to RGB colour [image, mode]. I'd then go to 'image, adjustments,
colour balance' and lightly adjust the colour balance of the selected region
and then "you can be brown, you can be blue, you can be violet sky", e.g.
move the colour balance slider from cyan to red and the selected object with
become progressively more red, with the underlying structural details
intact.
Then work through the entire image. Select all similar objects [to be the
same colour] within the image as one multiple region for colourizing [hold
shift as you select]. Make a mistake and use Edit, Undo. You can say
slightly adjust contrast and brightness, or curves, or shadow/highlights
within those selected regions as well.

You don't really need layers, you could work only on the main image
[background] bit by bit - save regularly under new file names as Photoshops
Undo [step backwards] is limited if, after a lot of work, you don't like the
way the image is turning out.

You could do all this with Photoshop Elements 8 as well [£37 with
discounts]. Do all the above Photoshop stuff using the similar Elements
selection tools, then [instead of 'colour balance'] go to: Enhance, Adjust
Colour, Adjust Hue/Saturation [and make sure the 'Colorize' box is ticked].

It will take a while to manually edit the entire image ['View, Zoom' to aid
tracing], but I doubt any image processing software could fully
'automatically' select the regions you want to colorize, particularly with a
greyscale SEM/TEM image. Photoshop's 'Quick selection tool' will have a go
though [the tools selection effect is adjustable in the upper menu bar].

I doubt the likes of alternative 'Pseudocolour' LUT effects will provide the
subtle colouring you require, although they may work adequately on TEM
images of sections.



Hope this helps,

Regards

Keith

---------------------------------------------------------------------------
Dr Keith J. Morris,
Molecular Cytogenetics and Microscopy Core,
Laboratory 00/069 and 00/070,
The Wellcome Trust Centre for Human Genetics,
Roosevelt Drive,
Oxford† OX3 7BN,
United Kingdom.

Telephone:† +44 (0)1865 287568
Email:† kjmorris-at-well.ox.ac.uk
Web-pages: http://www.well.ox.ac.uk/molecular-cytogenetics-and-microscopy




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Email: isha.mutreja-at-gmail.com
Name: isha mutreja

Organization: Ulster University

Title-Subject: [Filtered] Biomedical Sciences

Message: How to process SEM images by adobe photoshop to add coloured
effects to those images. Thank
you in anticipation. Looking forward for some help and guidance.

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From: tina-at-pbrc.hawaii.edu
Date: Thu, 5 Jan 2012 12:22:47 -0600
Subject: [Microscopy] Process SEM images by Adobe Photoshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Isha-

I tried replying in the thread, but the spam filter thought I had an
attachment and refused it, so Keith's instructions are not "below".

*************************************************************************
Yes, follow Keith's instructions below. Learn to use your selection tools
well, deciding, for example, if you want to feather the selection so it
looks more natural. I do like to use Selection - Save Selection so I can
save each selection as I go (and not lose all that hard work) and go back
and play with them later. Selectin the background first is often easiest,
and then you can select the inverse. You can use the Brush tool, the paint
bucket, or Edit - Fill, but in each case remember to select Color mode
instead of Normal (up on the menu bar). It is not automatic; it is time
consuming, but it can also be very therapeutic! These days, instead of
maintaining my MicroAngela website I make enamel jewelry under a
microscope... Same idea; surround an area with wire and then fill in with
colors. Yes, I did like coloring books when I was a child!

Aloha,
Tina
http://www5.pbrc.hawaii.edu/microangela/

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

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From: mary.raven-at-lifesci.ucsb.edu
Date: Thu, 5 Jan 2012 19:09:55 -0600
Subject: [Microscopy] LM - how to quench a GFP signal

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers
Thanks in advance for your time. A researcher here (see below) would like to quench her GFP signal. Any thoughts on how best to quench GFP with out disrupting the other antigens?

"Another question I have is if you known how to quench a GFP signal? I've been researching online and asking people and it seems that there isn't a good consensus on how to do it. I've heard that acetone fixation will work but I'm afraid that it might mess up my other antigens. I can't re-clone my virus, so I'm kind of stuck with the GFP...."

Happy New Year
Mary

Microscopy Facility Director
Neuroscience Research Institute&
Molecular, Cellular& Developmental Biology
Biology 2 Building, room 5173
The University of California
Santa Barbara, CA 93106-5060


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From: frank_karl-at-ardl.com
Date: Fri, 6 Jan 2012 07:58:43 -0600
Subject: [Microscopy] Upper magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Recently I've been asked a question about upper magnification range with the SEM. I know upper magnification is going to depend on each SEM, type of filament, skill of the operator, sample type, definitions of what's useful and other factors I haven't even thought of. But, as a general rule of thumb, what's the normal upper magnification with meaningful resolution (Definition: I can clearly see the features I want and would publish the image) for a ordinary hair pin filament SEM?

any thoughts?

Thanks!!!!

Frank Karl
Microscopist
ARDL
2887 Gilchrist Road
Akron, Ohio 44305
330-794-6600



________________________________
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.


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From: ce-at-personifysearch.com
Date: Fri, 6 Jan 2012 10:09:20 -0600
Subject: [Microscopy] Microscopy Marketing Manager Opportunity in Chicago

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Company:
Our client is a leading developer and producer of innovative high-tech
precision optics systems for the analysis of microstructures. As one of
the market leaders in each of the fields of Microscopy, Confocal Laser
Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical
Equipment. Comprising nine manufacturing facilities in seven countries,
sales and service companies in 20 countries and an international network
of dealers, the company is represented in over 100 countries.

The Opportunity:
The company currently has an opening for a Marketing Manager - Life
Science and Clinical to be based in Buffalo Grove/Chicago IL. All
applicants must not be adverse to travel, as this is a position that may
require you to travel when necessary.

Primary Responsibilities:
This role will be responsible for developing and building unique value
propositions that support the go to market strategy of the business. The
Marketing Manager will integrate and coordinate all functional activities
that are necessary to achieve and maintain sales and profitability within
the defined Market Segment.

If you meet and/or exceed the experience criteria, please visit our
website link (by clicking on link or by Copy and Paste) for the full job
description and to apply directly. We wish everyone the best of luck.
Unfortunately only qualified candidates will be considered.

http://www.pcrecruiter.net/pcrbin/direct.asp?r=g32EuzYwGqldTXX8YKm12%2bnCQ
uJrX%2fRZhDbXUQdrTys0Eda5SQ6H2lVL7OyAC9HHBC6uLhM%3d

Christy Edwards
Sr. e-Recruitment Consultant
Personify
Cary, NC 27513

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From: ce-at-personifysearch.com
Date: Fri, 6 Jan 2012 10:48:51 -0600
Subject: [Microscopy] Nanotechnology Sales Opportunity in Germany

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sales Representative - Nanotechnology

The Company:

Our client is a leading developer and producer of innovative high-tech
precision optics systems for the analysis of microstructures. As one of
the market leaders in each of the fields of Microscopy, Confocal Laser
Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical
Equipment. Comprising nine manufacturing facilities in seven countries,
sales and service companies in 20 countries and an international network
of dealers, the company is represented in over 100 countries.

The Opportunity:

The company currently has an opening for a Sales Representative -
Nanotechnology ideally based in Wetzlar, Germany. All applicants must not
be adverse to travel, as this is a position that will require travel
within the position territory.

Salary: 40,000 - 50,000 EUR plus bonus of 15,000 EUR

Other: Company Car - Pension Plan - Home Office - Laptop - Mobile Phone -
Internet paid by company

Primary Responsibilities:

- Direct sales of products for sample preparation for electron microscopy
in territory
- Acquisition of new customers
- Active support and consulting of the existing customers in industry and
research
- Presentation of preparation systems during trade shows, exhibitions,
workshops and customer demonstrations
- Budget responsibility and autonomous implementation of sales strategies

Education and Experience Required:

- University degree in natural science or comparable education
- Preferably 3 to 4 years of experience in sales of capital equipment
- Preferably job experience in the field of electron microscopy
- Ability to travel extensively and work independently
- Good command of English and excellent communications and negotiation
skills


If you meet and/or exceed the experience criteria, please submit your
resume by clicking on the link below or Copy and Paste link into browser.
We wish everyone the best of luck. Unfortunately only qualified
candidates will be considered.

http://tinyurl.com/Micro-SalesNano-Germany

Christy Edwards
Sr. e-Recruitment Consultant
Personify
5020 Weston Parkway
Suite 315
Cary, North Carolina 27513
www.personifysearch.com
Email: ce-at-personifysearch.com

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From: ehaller-at-health.usf.edu
Date: Fri, 6 Jan 2012 11:12:58 -0600
Subject: [Microscopy] Upper magnification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Frank,

Having taken about 20,000 biological SEM photos with a tungsten filament SEM, the useful top magnification for images of the surface of cells runs about 20,000x. You can push this to as much as 100,000x for organelles of ideally prepared samples if your instrument can go to 35-40kV and you are working with gold-palladium coated samples. I've imaged viruses budding from the surface of cells at 20-40kX before by SEM and had good photos. With material samples such as metals, where you have less beam penetration and more signal, you will get better signal to noise ratio and better high-end magnification photos, so 100,000x photos are not much of a problem. My microscope maxed out at 180,000x, and I could get grainy photos of my resolution sample at that magnification, but could still resolve 5nm on a tin ball on carbon sample.

Ed

Edward Haller, Lab Manager
University of South Florida
Integrative Biology Department
Electron Microscopy Core
SCA 110
4202 East Fowler Avenue
Tampa, FL 33620
813-974-2676
ehaller-at-usf.edu
Office: ISA 1046
________________________________________
X-from: frank_karl-at-ardl.com [frank_karl-at-ardl.com]
Sent: Friday, January 06, 2012 9:08 AM
To: Haller, Edward

Recently I've been asked a question about upper magnification range with the SEM. I know upper magnification is going to depend on each SEM, type of filament, skill of the operator, sample type, definitions of what's useful and other factors I haven't even thought of. But, as a general rule of thumb, what's the normal upper magnification with meaningful resolution (Definition: I can clearly see the features I want and would publish the image) for a ordinary hair pin filament SEM?

any thoughts?

Thanks!!!!

Frank Karl
Microscopist
ARDL
2887 Gilchrist Road
Akron, Ohio 44305
330-794-6600



________________________________
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.


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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 6 Jan 2012 18:38:42 -0600
Subject: [Microscopy] viaWWW:NESM February Meeting @ Saint-Gobain

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Title-Subject: [Filtered] SAVE-THE-DATE: NESM February Meeting -at- Saint-Gobain

Message: Greetings Confocal Listserv-ites,

This is your friendly SAVE-THE-DATE reminder for NESM's February Dinner Meeting hosted by
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Technical Talks:

"Crystals as Stacked Layers and the Infinite World of Intergrowths", Charles Bateman, Ph.D.,
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"Microscopy tools: visual resolution of insect eyes", Paloma Gonzalez Bellido, Ph.D., Marine
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From: bozzola-at-siu.edu
Date: Sat, 7 Jan 2012 09:30:37 -0600
Subject: [Microscopy] Upper magnification

Contents Retrieved from Microscopy Listserver Archives
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As with the SEM of Ed Haller, our 28 y/o Hitachi S570 gave reasonably
good images up to 100KX. I believe this is probably a good expectation
for most tungsten-filament based SEMs. Some might give even better,
while others (like entry level or variable pressure instruments) may
not perform as well.

Of course, trimming the SEM (alignment, stigmation, good vacuum,
stable and conductive specimen) will have a tremendous effect on the
final image.

Cheers,
--
John J. Bozzola, Ph.D., Professor & Director of IMAGE (Retired : -)
Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL  62901
Phone: 618-453-3730


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From: oshel1pe-at-cmich.edu
Date: Mon, 9 Jan 2012 14:09:16 -0600
Subject: [Microscopy] intensifying a FRET signal

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} Date: Mon, 9 Jan 2012 12:02:29 -0800
} Reply-To: Venu Polineni {vens06-at-gmail.com}
} Subject: Ask-A-Microscopist
}
} realname - Venu Polineni
} Email - vens06-at-gmail.com
} EDUCATION - Graduate College
} SUBJECT_OF_QUESTION - FRET
} QUESTION - Is there a specific chemical like saponin to intensify a
} FRET signal? Or are there specific agents/detergents/fixatives that
} either intensify or bleach a FRET signal.
}
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From: john.oreopoulos-at-utoronto.ca
Date: Mon, 9 Jan 2012 14:31:13 -0600
Subject: [Microscopy] Re: intensifying a FRET signal

Contents Retrieved from Microscopy Listserver Archives
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Venu,

Look up this paper:

Rodighiero, S., et al., Fixation, mounting and sealing with nail polish of cell specimens lead to incorrect FRET measurements using acceptor photobleaching. Cellular Physiology and Biochemistry, 2008. 21(5-6): p. 489-498.

John Oreopoulos
Research Assistant
Spectral Applied Research
Richmond Hill, Ontario
Canada
www.spectral.ca


On 2012-01-09, at 3:15 PM, oshel1pe-at-cmich.edu wrote:

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} } Subject: Ask-A-Microscopist
} }
} } realname - Venu Polineni
} } Email - vens06-at-gmail.com
} } EDUCATION - Graduate College
} } SUBJECT_OF_QUESTION - FRET
} } QUESTION - Is there a specific chemical like saponin to intensify a
} } FRET signal? Or are there specific agents/detergents/fixatives that
} } either intensify or bleach a FRET signal.
} }
} --
}
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 10 Jan 2012 19:45:23 -0600
Subject: [Microscopy] viaWWW:Anti-static medicine cups

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Email: yamawaki-at-stanford.edu Name: Ruth Yamawaki

Organization: Stanford University

Title-Subject: [Filtered] Anti-static medicine cups

Message: We used polypropylene specimen cups (static-free) to prepare the water in the boats for
sectioning. They made water static-free. Our serial sections did not move around the boat.

Now these cups do not work. There has been a change in the manufacturing of the cups so they no
longer have the ability to make our water static free.

Has anyone else come up with a way to solve this problem?

Thank you,
Ruth Yamawaki
Stanford University
Department of Comparative Medicine
650-723-3457

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From: tbargar-at-unmc.edu
Date: Wed, 11 Jan 2012 09:15:22 -0600
Subject: [Microscopy] spare parts for LKB-Pyramitome model 11800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I have a colleague at Creighton University who is trying to repair a
LKB-Pyramitome model 11800 and needs a "toothed" belt. Can anyone help?
She doesn't subscribe to this listserver so I'll pass along any
information to her. Thanks.

Tom Bargar
University of Nebraska Medical Center
Core Electron Microscopy Research Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu

==============================Original Headers==============================
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From: vray-at-partbeamsystech.com
Date: Wed, 11 Jan 2012 10:51:07 -0600
Subject: [Microscopy] Re: spare parts for LKB-Pyramitome model 11800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tom,

I am not familiar with the particular model, but if dimensions of the
belt can be measured or deducted from the design of system then good
places to start looking for it may be:

http://www.smallparts.com/b/16411421/ref=sp_iss_16411421

http://www.mcmaster.com/#timing-belts/=fre4ok

In unlikely case that McMaster and SmallParts would not have what you
need - try to Google for "timing belt", I am sure that there are plenty
of other places to get it.

Cheers :)
Valery Ray
=================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844 USA
Phone: +1-978-296-5063 - leave a message with call-back number
US Mobile: +1-978-305-0479
Skype: pbstmeo
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 1/11/2012 10:16 AM, tbargar-at-unmc.edu wrote:
} ----------------------------------------------------------------------------
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} I have a colleague at Creighton University who is trying to repair a
} LKB-Pyramitome model 11800 and needs a "toothed" belt. Can anyone help?
} She doesn't subscribe to this listserver so I'll pass along any
} information to her. Thanks.
}
} Tom Bargar
} University of Nebraska Medical Center
} Core Electron Microscopy Research Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
} ==============================Original Headers==============================
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From: nholson-at-ucsd.edu
Date: Wed, 11 Jan 2012 15:39:25 -0600
Subject: [Microscopy] Availability of Kodak EM film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


--
I know that most EM labs have moved away from EM film although quite
a few labs in my field still use it. I just got an email from my
vendor who gave me an update on Kodak film. I am posting that
message in its entirety. I thought that a number of other users on
the list might like this information so I hope I am not crossing the
line into advertising.

Norm Olson
University of California San Diego
***********************
Norm-

Good Afternoon
Below is an email from my Kodak Product Manager about the Kodak
SO-163 EM film you purchase from us. I have had a number of calls
pertaining to the film in the last few days since Kodak announced
their probability of filing bankruptcy.
Please don't hesitate to call or email me if there are any additional
questions you may have. I have stock available if you need to place
an order.
Best Regards,
Sheila



Sheila Danahy
Sales Representative
Aremac Holdings
Tritech Forensics
Phone #866-972-6464 X 3128
Fax #866-682-0940
www.tritechforensics.com


KODAK Electron Microscope 4489 and KODAK Electron Image Film SO-163
are manufactured and sold by Carestream Molecular Imaging. You can
assure your customers that these products will be readily available
in the future as far as we can see.

Some background information. When Eastman Kodak Company spun off the
health sciences part of their business which became Carestream Health
in 2007, Carestream Health purchased all the film factories and
manufacturing equipment as well as retaining all the skilled staff to
continue producing the film products. Based on the value of the
Kodak name, Carestream has an agreement with Eastman Kodak Company to
sell the product under the Kodak name.

The film is made on the same machines by the same talented
manufacturing teams by Carestream Health. You can inform customers
you have spoken directly with the film source company and the EM film
is and will be available for them whenever they need it.

Please do not hesitate to contact me if I can be of assistance on any matter.

Best regards,
Eric

Eric Ambrose
Product Manager - Media Products
Carestream Molecular Imaging

eric.ambrose-at-carestream.com
P 585-627-8762
M 585-330-1646

Carestream Health, Inc.
150 Verona Street
Rochester, NY 14608







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From: woody-at-albe24.com
Date: Wed, 11 Jan 2012 16:44:39 -0600
Subject: [Microscopy] Re: spare parts for LKB-Pyramitome model 11800

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If the belt can be measured, here are two sources to check:
http://www.smallparts.com
or
http://www.pic-design.com

Woody
}
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} I have a colleague at Creighton University who is trying to repair a
} LKB-Pyramitome model 11800 and needs a "toothed" belt. Can anyone help?
} She doesn't subscribe to this listserver so I'll pass along any
} information to her. Thanks.
}
}


--


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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 11 Jan 2012 20:59:51 -0600
Subject: [Microscopy] viaWWW:intensify or bleach a FRET signal

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Title-Subject: [Filtered] Developmental Biology

Message: Are there specific agents which can either intensify or bleach a FRET signal??

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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 11 Jan 2012 21:00:31 -0600
Subject: [Microscopy] viaWWW:Job Opportunity: Lab Mgr. for electron microprobe lab

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Email: mmh-at-umn.edu Name: Marc Hirschmann

Organization: University of Minnesota

Title-Subject: [Filtered] Job Opportunity: Lab Mgr. for electron microprobe lab

Message: Electron Microprobe Research Scientist, University of Minnesota, Minneapolis
The Department of Earth Sciences is seeking applications for an Electron Microprobe Research
Scientist. Responsibilities will include operation, supervision and routine maintenance of a
5-spectrometer JEOL 8900 electron microprobe. Experience analyzing geological samples by EMPA is
essential. Some experience maintaining a microprobe is desirable, but some amount of on-the-job
training is expected and the instrument continues to be on a JEOL service contract. The successful
applicant will also be expected to provide user training, including teaching a course in microprobe
theory and applications, and to consult/collaborate with University researchers and students on
research projects. Additionally, the position requires providing support for a moderate load of
industrial users. The position also provides opportunities to conduct independent research using the
microprobe as well as other facilities available at UMN.
An M.S. or Ph.D. degree in Earth Science or related field is required. This non-tenure track
position is available as a Research Fellow (M.S. degree, requisition #172657), or a Postdoctoral
Associate (up to 3 years experience post PhD, requisition #172658), or a Research Associate (3 years
post PhD experience, requisition #175602). To apply, candidates should go to
https://employment.umn.edu and attach a cover letter, resume (CV), statement of research interests,
and names/contact information of three references. Application review will begin on March 15,
2012, and will continue until the position is filled. For further information, contact Marc
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 11 Jan 2012 21:12:43 -0600
Subject: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?

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Email: mchimento-at-uab.edu Name: Melissa Chimento

Organization: University of Alabama at Birmingham

Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?

Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
they could check dispersion of the ND in the polymer with it??


Thanks!


Melissa Chimento

University of Alabama at Birmingham-HRIF

Department of Vision Science

205-934-1926


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From: lieberw-at-mpip-mainz.mpg.de
Date: Thu, 12 Jan 2012 01:21:22 -0600
Subject: [Microscopy] Re: viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?

Contents Retrieved from Microscopy Listserver Archives
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Dear Melissa,

sectioning a polymer is not really a big deal if you have a (cryo)-
microtome. However, as always the devil is in the details!
First you need to know the TG (Glasstransition Temperature) of the
polymer in question. If itīs below RT you should prepare to do some
cryo cutting. If not, so much the better.
The real problem will be the ND, they will surely damage your knife
and will be bunked out of the polymer matirix to some extent.
However, you can try using a glass knife or I would suggest to use an
used diamond knife, where you donīt mind a few more additional
scratches.
Just give it a try, itīs not that difficult if you know how to
operate a microtome.

Cheers
Ingo
}
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} Email: mchimento-at-uab.edu Name: Melissa Chimento
}
} Organization: University of Alabama at Birmingham
}
} Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
}
} Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
} introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
} polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
} NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
} suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
} the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
} they could check dispersion of the ND in the polymer with it??
}
}
} Thanks!
}
}
} Melissa Chimento
}
} University of Alabama at Birmingham-HRIF
}
} Department of Vision Science
}
} 205-934-1926
}
}
} Login Host: 138.26.156.111
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--------------------------------------------------
Ingo Lieberwirth
Max Planck Institute for Polymer Research
Ackermannweg 10
D-55128 Mainz

Tel.: ++49 6131 379 580
Fax.: ++49 6131 379 100
--------------------------------------------------



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From: sckuehn-at-concord.edu
Date: Thu, 12 Jan 2012 07:37:52 -0600
Subject: [Microscopy] List of labs - electron microprobes

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Colleagues,

Apologies if you receive this more than once as it is going to multiple
lists.

Over the last ~18 months, I have been setting up a new electron
microprobe lab at a 2800-student primarily undergraduate university in
West Virginia. Being curious if there might be any other undergraduate
schools with a fully-functioning probe lab, I prepared a compilation of
North American probe labs. Through this exercise, I think I have found
one other undergraduate probe lab.

Wanting to share, I have posted the compilation on our lab website at:
http://academics.concord.edu/microanalysis/OtherLabs.html

The compilation includes a Google Maps view as well as tables listing
the labs by institution name and by instrument type. Both academic and
government/industry labs are included. Also included are links to the
lab web sites where I have them.

Perhaps some of you may find this recent compilation to be useful.

I also could use your help in checking the accuracy and completeness of
the compilation. Please take a look and suggest additions/changes as
needed. I am sure that I have missed a few.

Thanks and best regards,

- Steve Kuehn

--

----------------
Dr. Stephen C. Kuehn
Research Assistant Professor
Manager, Electron Microprobe Facility & Tephra Lab
Science building, Room 106

Concord University
1000 Vermillion St
PO Box 1000, Campus Box F20
Athens, WV 24712-1000

http://academics.concord.edu/sckuehn/
http://academics.concord.edu/microanalysis/
sckuehn-at-concord.edu kuehnsc-at-gmail.com
304-384-6322

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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 12 Jan 2012 08:28:09 -0600
Subject: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Melissa,
I agree with Ingo, my first try would cryo-UM depending on the polymer matrix. The only problem is
that the diamond particles are going to fall out of the material and leave nice big gouges in the
sections. Another possible solution would be a cryo-fracture and hi-res SEM of the material. You may
have to thin the brick down to a manageable thickness in order to fracture it. Good luck,
Vicky

Victoria M. Bryg
Research Associate - NCSER/USRA
NASA Glenn Research Center
216-433-9628



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} From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com]
Sent: Wednesday, January 11, 2012 10:20 PM
To: Bryg, Victoria M. (GRC-REC0)[National Center for Space Exploration Research]

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Email: mchimento-at-uab.edu Name: Melissa Chimento

Organization: University of Alabama at Birmingham

Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?

Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
they could check dispersion of the ND in the polymer with it??


Thanks!


Melissa Chimento

University of Alabama at Birmingham-HRIF

Department of Vision Science

205-934-1926


Login Host: 138.26.156.111
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From: modla-at-dbi.udel.edu
Date: Thu, 12 Jan 2012 08:34:36 -0600
Subject: [Microscopy] Durcupan resin

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Recently, we have noticed more reports of using Durcupan ACM resin in the literature, so we ordered a Durcupan ACM resin kit to try out. I noticed this resin is very viscous, much more so than the Embed-812 resin that we typically use. Does anyone have any particular resin infiltration schedules that have worked well for them for cell cultures and plant and animal tissues? I read that Durcupan causes less shrinkage than other resins, but does it have any other advantages over other resins for certain applications?

Thanks,
Shannon
Bio-Imaging Center
Delaware Biotechnology Institute
Newark, DE

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From: jacques.faerber-at-ipcms.u-strasbg.fr
Date: Thu, 12 Jan 2012 10:34:39 -0600
Subject: [Microscopy] Re: viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?

Contents Retrieved from Microscopy Listserver Archives
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Hi Melissa

I would try SEM on a cry-fractured sample.

To fracture the sample, deep it in liquid nitrogen, until le nitrogen
stops to boil. Then, take it out and put in on an amboss, and hit it
with a hammer. To avoid the flakes to fly everywhere, you can put some
weeping paper on the amboss that you fold to cover the sample before you
hit it. You must do all these steps fast, to limit the reheating of the
sample.
It's not a very academic methode, nor it is very reproductible, but it
works. You can help the fracturing at a wanted place by making a notch
with a rasor blade. The way it will break depends of the type of the
polymer.

You choose in the flakes some flat pieces for the observation and coat
them with carbon. If the ND are really nano, you will need low energy
and high resolution. If the "nano" is in the 100 nm range or more, BSE
detector, higher energy (and maybe ESEM mode) should work.

Hope it helps

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matťriaux de Strasbourg
Dťpartement Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


Le 12/01/2012 04:22, microscopylistserver-noreply-at-microscopy.com a ťcrit :
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}
} Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
}
} Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
} introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
} polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
} NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
} suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
} the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
} they could check dispersion of the ND in the polymer with it??
}
}
} Thanks!
}
}
} Melissa Chimento
}
} University of Alabama at Birmingham-HRIF
}
} Department of Vision Science
}
} 205-934-1926
}
}
} Login Host: 138.26.156.111
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From: jpapalia-at-papalia.net
Date: Thu, 12 Jan 2012 10:38:53 -0600
Subject: [Microscopy] Re: [Filtered] RE: viaWWW:Nano diamonds dispersed in a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Perhaps instead of EM you might be better off investigating via
scattering, such as SAXS? That way you could look at the bulk sample and
see an average particle spacing, if the technique would permit it
(depends on density/spacing of NDs in the polymer).

Alternatively, instead of positive evidence (seeing the NDs) is your
work flexible enough to allow for the use of negative evidence instead
of positive evidence? In other words, if the NDs all get popped out
during microtoming you end up with a system full of voids. Investigation
of void spacing could indirectly give you info on ND spacing. This would
be dependent on how cleanly the NDs come out of the polymer - if they
tear or gouge the sample badly, then this would be a failed method.

-John Papalia

**********************
John M. Papalia, Ph.D.
Materials Scientist & Job seeker
**********************


On 1/12/2012 9:35 AM, microscopylistserver-noreply-at-microscopy.com wrote:
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} Dear Melissa,
} I agree with Ingo, my first try would cryo-UM depending on the polymer matrix. The only problem is
} that the diamond particles are going to fall out of the material and leave nice big gouges in the
} sections. Another possible solution would be a cryo-fracture and hi-res SEM of the material. You may
} have to thin the brick down to a manageable thickness in order to fracture it. Good luck,
} Vicky
}
} Victoria M. Bryg
} Research Associate - NCSER/USRA
} NASA Glenn Research Center
} 216-433-9628
}
}
}
} -----Original Message-----
} } From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com]
} Sent: Wednesday, January 11, 2012 10:20 PM
} To: Bryg, Victoria M. (GRC-REC0)[National Center for Space Exploration Research]
} Subject: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?
}
}
}
}
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} Email: mchimento-at-uab.edu Name: Melissa Chimento
}
} Organization: University of Alabama at Birmingham
}
} Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
}
} Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
} introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
} polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
} NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
} suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
} the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
} they could check dispersion of the ND in the polymer with it??
}
}
} Thanks!
}
}
} Melissa Chimento
}
} University of Alabama at Birmingham-HRIF
}
} Department of Vision Science
}
} 205-934-1926
}
}
} Login Host: 138.26.156.111
} ---------------------------------------------------------------------------
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}
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From: John.Mardinly-at-asu.edu
Date: Thu, 12 Jan 2012 10:40:02 -0600
Subject: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?

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Use a FIB.

John Mardinly,
ASU



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Email: mchimento-at-uab.edu Name: Melissa Chimento

Organization: University of Alabama at Birmingham

Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?

Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
introduce the NDs before polymerization at room temperature. They said that it forms a ¬"brick¬" of
polymer. I¬'m not sure what to do with the sample. I¬'ve had people bring in electro spun fibers with
NDs but not a solid chunk. From what I¬'ve read polymers are extremely difficult to thin section. Any
suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM¬'s in
the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I¬'m not sure if
they could check dispersion of the ND in the polymer with it??


Thanks!


Melissa Chimento

University of Alabama at Birmingham-HRIF

Department of Vision Science

205-934-1926


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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 13 Jan 2012 08:25:35 -0600
Subject: [Microscopy] [Filtered] Re: viaWWW:Nano diamonds dispersed in a polymer

Contents Retrieved from Microscopy Listserver Archives
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Melissa,

What size are the nanodiamonds are expected to be? If they in the 2 to 5 nm range, and the loading
fraction is not too high (~10% or lower by volume), microtoming should work just fine (cryo if Tg { RT).
At a 70 or 100 nm slice thickness, the vast majority should be well encapsulated with polymer, and
not drop out. A diamond microtome knife shouldn't get hurt either, unless the NDs are a bit bigger,
i.e., } 10 nm.
We do this routinely for some naturally-occurring polymer-nanodiamond samples, and haven't had to
change the diamond knife at all.

For } 10 nm NDs, I would go a with the FIB, as John said, either for direct-slice and view in the
FIB if you can get enough contrast, or for TEM imaging if contrast is a problem.
For the smaller NDs the surface damage and re-dep may make it hard to get the contrast you need for
either FIB-based or TEM-based imaging, unless you have a really good FIB and FIB operator.

Good luck.

Rhonda

On 1/11/2012 10:13 PM, microscopylistserver-noreply-at-microscopy.com wrote:
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}
} Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
}
} Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
} introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
} polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
} NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
} suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
} the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
} they could check dispersion of the ND in the polymer with it??
}
}
} Thanks!
}
}
} Melissa Chimento
}
} University of Alabama at Birmingham-HRIF
}
} Department of Vision Science
}
} 205-934-1926
}
}
} Login Host: 138.26.156.111
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--
__________________________________
Head, Nanoscale Materials Section
Code 6366
Naval Research Laboratory
4555 Overlook Avenue SW
Washington, DC 20375
(v) 202-404-4143
(f) 202-767-1697
rhonda.stroud-at-nrl.navy.mil
__________________________________







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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 13 Jan 2012 08:25:46 -0600
Subject: [Microscopy] [Filtered] Re: Nano diamonds dispersed in a polymer TEM?SEM?

Contents Retrieved from Microscopy Listserver Archives
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Hi Melissa,

As a reality-sanity check, before preparing the polymer/diamond sample, I would suggest trying to
image a suspension of the nano-diamonds on a carbon film support. This is just to be certain of the
size of the nano-diamonds. Their size will influence how sliceable they may be with a diamond
knife. If they are very small, really nano sized, they may stay put in the polymer and make a nice
sample. The larger they are the higher the probability of pull out and knife damage. It would also
be good to ask what the researchers think the concentration of nano-diamond is in the polymer.

Years ago I had many researchers ask me to image what they thought were 5-10 nm diameter (lab-made)
nano-particles, these were often 100-700 nm diameter particles. This does not happen much anymore
but it is sometimes good to check before investing hours of your time. Also nano diamonds in
polymer may be easier to image with STEM dark field imaging rather than TEM--if you have the option.

They may want to try cryo-FIB of a block of their sample. It might be possible to get that done by
JEOL, FEI --not sure if Hitachi has a cryo FIB. IF the manufacturer can show off the results for
marketing purposes they will sometimes do exploratory samples for free.

Good luck,
Roseann

Roseann Csencsits, PhD
Scientist in Charge, Donner TEM Facility
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548










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}
} Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?
}
} Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
} introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
} polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
} NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
} suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
} the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
} they could check dispersion of the ND in the polymer with it??
}
}
} Thanks!
}
}
} Melissa Chimento
}
} University of Alabama at Birmingham-HRIF
}
} Department of Vision Science
}
} 205-934-1926
}
}
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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 13 Jan 2012 08:26:15 -0600
Subject: [Microscopy] viaWWW:Nano diamonds dispersed in a polymer TEM?SEM?

Contents Retrieved from Microscopy Listserver Archives
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I greatly appreciate all of the feedback I received to this post.
Thank you,

Melissa F. Chimento
University of Alabama at Birmingham
HRIF SHEL Room 912
1825 University Boulevard
Birmingham, AL 35294

LAB #: (205) 934-1926
OFFICE #: (205) 996-6469

mchimento-at-uab.edu



-----Original Message-----
} From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com]
Sent: Wednesday, January 11, 2012 9:20 PM
To: Melissa Foley Chimento

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Email: mchimento-at-uab.edu Name: Melissa Chimento

Organization: University of Alabama at Birmingham

Title-Subject: [Filtered] Nano diamonds dispersed in a polymer TEM?SEM?

Message: We have a group that wants to see how nano diamonds(NDs) disperse in a polymer. They
introduce the NDs before polymerization at room temperature. They said that it forms a ¬ďbrick¬Ē of
polymer. I’m not sure what to do with the sample. I’ve had people bring in electro spun fibers with
NDs but not a solid chunk. From what I’ve read polymers are extremely difficult to thin section. Any
suggestions about how to evaluate ND dispersion in a solid would be appreciated. We have 2 TEM’s in
the lab. Should I try cryo-sectioning the material? We have a new ESEM on campus. I’m not sure if
they could check dispersion of the ND in the polymer with it??


Thanks!


Melissa Chimento

University of Alabama at Birmingham-HRIF

Department of Vision Science

205-934-1926


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From: parishcm-at-ornl.gov
Date: Fri, 13 Jan 2012 10:49:43 -0600
Subject: [Microscopy] EBSD: prepping Zircaloy

Contents Retrieved from Microscopy Listserver Archives
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Any advice for EBSD of zircaloys? I tried multi-hour colloidal silica (no patterns, 15-30 kV) and then tried a few minutes of colloidal silica+ammonium hydroxide + hydrogen peroxide (still no patterns, 15-30 kV).

Has anyone had good luck? My next resort is our Gatan Ilion ion polisher, but I'm hoping for a simple answer to let me use my already mounted and polished specimens.

Thanks,
Chad Parish



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From: stefan.diller-at-t-online.de
Date: Mon, 16 Jan 2012 05:47:05 -0600
Subject: [Microscopy] Classifying diatomee

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
maybe somebody out there can help me with classifying this diatomee:
http://www.electronmicroscopy.info/diatomee/index.htm

I found it on a ca 300 year old piece of slag from iron smelting in a valley in the northern part of Bavaria, Germany. The small
river I found it in is not too warm during the year, height above MSL is ca. 800 to 400 meter.
Google earth link is at
http://www.electronmicroscopy.info/diatomee/iron_slug.kmz

Thanks,
Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
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From: frank_karl-at-ardl.com
Date: Mon, 16 Jan 2012 07:57:54 -0600
Subject: [Microscopy] microtoming silicone rubber for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

That a great way to start a work week: A diatom question! Unfortunately I don't have any of my diatom references at work, but I have a question of my own.

I cutting cryo-thin sections of silica loaded silicone rubber with a diamond histology knife (it's all I have). It as a Tg of -127C. I'm working dry at -140C using sugar water to pick-up the thin sections for TEM imaging at 80kv. I don't know the loading of silica in the silicone polymer. The microtome is a RMC microtome with cryo-stage.

It's not going well. I can't seem to get to 100 nm. The color guide, based on the interference of light, suggests I at 280nm. In the few thin spots or tears I can see the particles of silica I'm after but the rest of the section is too thick.

Has anyone practical experience with cryo microtoming of silica loaded silicone?
I'm open to ideas

Thanks....
Frank









________________________________
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.


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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 16 Jan 2012 09:00:43 -0600
Subject: [Microscopy] viaWWW:Position Vacant - Postdoctoral Research Associate - In Situ

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Email: fabrice.noel-at-sydney.edu.au Name: Fabrice Noel

Organization: The University of Sydney

Title-Subject: [Filtered] Position Vacant - Postdoctoral Research Associate - In Situ Deformation TEM

Message: Dear all,

The Faculty of Engineering at the University of Sydney is currently seeking a Postdoctoral Research
Associate.
Visit sydney.edu.au/positions and search by the reference number for more information and to apply.


POSTDOCTORAL RESEARCH ASSOCIATE - IN-SITU DEFORMATION TEM
SCHOOL OF AEROSPACE, MECHANICAL AND MECHATRONIC ENGINEERING
REFERENCE NO. 1995/1111

¬ē Well established faculty
¬ē Cutting-edge research
¬ē Work with internationally renowned academics

We currently seek a Postdoctoral Research Associate to work on an ARC funded project. This project
aims to apply state-of-the-art in-situ deformation transmission electron microscopy techniques to
reveal how crystalline defects in nanostructured metals and alloys interact with each other and to
link directly the interactions with the mechanical behaviour of the materials. The results will
enable structural design of advanced metallic materials with optimum mechanical properties.

This is an opportunity to work closely with Associate Professor Xiaozhou Liao and conduct research
at the Centre for Advanced Materials Technology (CAMT) within the school. You will be part of a
school which has a high international profile for its quality research over a wide field in
materials characterisation and processing, nanotechnology, advanced manufacturing, solid mechanics
and biotechnology.

Having completed a PhD in Material Science (or relevant area), you will have extensive experience in
transmission electron microscopy (TEM) with a solid background and understanding in the structure
and mechanical properties of materials.
CLOSING DATE: 14 February 2012 (11:30pm Sydney time) or until a successful individual has been
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From: frank_karl-at-ardl.com
Date: Tue, 17 Jan 2012 07:39:05 -0600
Subject: [Microscopy] Classifying diatomee

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stefan,
I pulled out my only reference to European diatoms, Heurch's Treatise on Diatomaceae published in 1896. I just have a photographic copy of it so the images aren't that sharp and the line drawings are so-so but I think your diatom best matches Navicula viridula.

hope this helps .....
Frank

-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: Monday, January 16, 2012 7:00 AM
To: Frank Karl

Dear All,
maybe somebody out there can help me with classifying this diatomee:
http://www.electronmicroscopy.info/diatomee/index.htm

I found it on a ca 300 year old piece of slag from iron smelting in a valley in the northern part of Bavaria, Germany. The small
river I found it in is not too warm during the year, height above MSL is ca. 800 to 400 meter.
Google earth link is at
http://www.electronmicroscopy.info/diatomee/iron_slug.kmz

Thanks,
Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

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==============================Original Headers==============================
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From: eikonika-at-otenet.gr
Date: Tue, 17 Jan 2012 08:10:15 -0600
Subject: [Microscopy] microorganism to identify

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers
Looking at a mucosal animal epithelium with SEM, I came across a creature
that is strange to me. It's like 30 microns long tube at the shape of L,
the short arm has a hole at the end and the long one is twisted like a
screw. Maybe it is a short of borrelia? Whoever is familiar with
microorganisms, please have a look at

http://www.eikonika.net/v2/photo_list_nikas.php

Any comments will be greatly appreciated!

Best regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
eikonika-at-otenet.gr


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From: stefan.diller-at-t-online.de
Date: Tue, 17 Jan 2012 09:37:14 -0600
Subject: [Microscopy] Classifying diatomee - solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks all for your help concerning my diatomee problem.

The most probable outcome is "Hippodonta capitata", as suggested by Prof. Hans Rudolf Thierstein.
He also pointed me to a really impressing source on diatomee:
http://westerndiatoms.colorado.edu/taxa/species/hippodonta_capitata

Best wishes,
Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
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www.elektronenmikroskopie.info
www.assisi.de
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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From: wesaia-at-iastate.edu
Date: Tue, 17 Jan 2012 09:55:14 -0600
Subject: [Microscopy] Classifying diatomee - solved

Contents Retrieved from Microscopy Listserver Archives
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Maybe this question would be best for Professor Thierstein, but is there a good online guide for classifying diatoms?

I have looked at quite a few diatoms in water samples over the years but have not embarked on trying to classify them. They were simply a significant part of the solids load of the water sample. Many looked like your sample.

However, from a quick search prompted by your question, I see similarities between hippodonta, planothidium, and navicula. The differences appear to be subtle between them. Therefore, I wonder about a good, definitive classification scheme.

Warren Straszheim


-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: Tuesday, January 17, 2012 9:38 AM
To: wesaia-at-iastate.edu

Thanks all for your help concerning my diatomee problem.

The most probable outcome is "Hippodonta capitata", as suggested by Prof. Hans Rudolf Thierstein.
He also pointed me to a really impressing source on diatomee:
http://westerndiatoms.colorado.edu/taxa/species/hippodonta_capitata

Best wishes,
Stefan


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From: FMonson-at-wcupa.edu
Date: Tue, 17 Jan 2012 13:23:22 -0600
Subject: [Microscopy] Classifying diatomee - solved

Contents Retrieved from Microscopy Listserver Archives
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This, and many more Diatomacea tracts, might also help - even if specified for US.

http://books.google.com/ebooks?id=G35IAAAAYAAJ&dq=diatoms%20of%20united%20states%20subject%3A%22Science%22&lr&as_brr=5&ei=56oVT8eHDoq0NtiY3LsG&source=webstore_bookcard

http://books.google.com/ebooks?id=mzYDAAAAQAAJ&dq=diatoms%20of%20united%20states%20subject%3A%22Science%22&lr&as_brr=5&ei=26sVT4eAKIz6M5KvpOoB&source=webstore_bookcard Paleontology by Richard Owen

http://books.google.com/ebooks?id=rZY_AAAAYAAJ&dq=diatoms%20of%20united%20states%20subject%3A%22Science%22&lr&as_brr=5&ei=ickVT-WcJoPUM4e8re0L&source=webstore_bookcard (A Treatise on the Diatomacea)

Cheers,

FCM

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl

If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state.
1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008.
2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224


-----Original Message-----
X-from: stefan.diller-at-t-online.de [mailto:stefan.diller-at-t-online.de]
Sent: Tuesday, January 17, 2012 10:44 AM
To: Monson, Frederick

Thanks all for your help concerning my diatomee problem.

The most probable outcome is "Hippodonta capitata", as suggested by Prof. Hans Rudolf Thierstein.
He also pointed me to a really impressing source on diatomee:
http://westerndiatoms.colorado.edu/taxa/species/hippodonta_capitata

Best wishes,
Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 17 Jan 2012 20:03:51 -0600
Subject: [Microscopy] viaWWW:Wanted, TEM

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From: beth-at-plantbio.uga.edu
Date: Wed, 18 Jan 2012 11:25:30 -0600
Subject: [Microscopy] hpf - dinoflagellates

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
A student wants to use high pressure freezing on dinoflagellates (size
approx. 10 microns). Has anyone done this? If yes, did you wick the
dinos into cellulose capillary tubes prior to freezing? Or, is there a
better method for keeping track of them?

thanks,
Beth


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From: michael-at-shaffer.net
Date: Wed, 18 Jan 2012 13:30:07 -0600
Subject: [Microscopy] =?windows-1252?Q?SEM=3A_how_much_larger_are_bright_particles_tha?=

Contents Retrieved from Microscopy Listserver Archives
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Having installed a FEG image analyzer and comparing its results with our tungsten SEM, we are seeing a slight bias with respect to brighter particles measuring smaller (possibly more accurately) with the FEG. The image is backscattered electrons and we use a fixed background threshold for isolating particles from the epoxy. It occurred to me that clipping the edge at the same background value would make brighter particles larger depending on how "fuzzy" the edge is. It would even be possible to predict this "bright versus dark" bias if the SEM's spot size was determined. Some difference should be associated with each SEM's magnification calibration, but I'm accounting for that by first relating calibration to the darkest minerals (quartz which is abundant).

I thought before I re-invent the wheel and get involved with the math and all the other possible variables (e.g., pixel resolution, irregular perimeters, ...) that I'd ask my peers if this has been done before(?) ... leastwise, I haven't yet found a reference ...

TIA & Cheerios from the Avalon
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Michael Shaffer
SEM-MLA Research Coordinator
Bruneau Centre for Research & Innovation
Memorial University
St. John's, Newfoundland, Canada
Information: http://www.mun.ca/creait/maf/
Scheduling: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
(709) 864-6799 (Ofc)
(709) 864-6790 (Lab)
cogito ergo ZzoooomM



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From: wesaia-at-iastate.edu
Date: Wed, 18 Jan 2012 13:49:22 -0600
Subject: [Microscopy] SEM: how much larger are bright particles tha

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That doesn't quite make sense to me. I don't see how changing to a higher resolution would lead to what you describe.

In a previous research life, I did a lot of image analysis on minerals in coal. The brighter ones (primarily pyrite) were cut much lower than half max when the threshold was set to detect the darker minerals which comprised the bulk of the mineral matter. Therefore, I expected the pyrite to be measured larger than actual size. The amount of error related to the spot size and the "fuzziness" of the signal. However, the effect was far beyond what I would expect the difference to be between W and field-emission guns.

I would look into other things that might have changed with the changeover.

Is the response of the BSE signal the same, or have they introduced some non-linearity that might have changed how the threshold is set? I would be interested in seeing the relative brightness of the various minerals under similar setups.

Has the software adapted some sort of local thresholding? I know I would have appreciated that back in the day, but we didn't have the computing power to well implement it.

I'd be interested in continuing the conversation off-line if that would be more expedient.

Warren Straszheim
former operator of a LeMont Scientific DB-10 image analyzer, circa 1981.
________________________________________
X-from: michael-at-shaffer.net [michael-at-shaffer.net]
Sent: Wednesday, January 18, 2012 1:30 PM
To: wesaia-at-iastate.edu

Having installed a FEG image analyzer and comparing its results with our tungsten SEM, we are seeing a slight bias with respect to brighter particles measuring smaller (possibly more accurately) with the FEG. The image is backscattered electrons and we use a fixed background threshold for isolating particles from the epoxy. It occurred to me that clipping the edge at the same background value would make brighter particles larger depending on how "fuzzy" the edge is. It would even be possible to predict this "bright versus dark" bias if the SEM's spot size was determined. Some difference should be associated with each SEM's magnification calibration, but I'm accounting for that by first relating calibration to the darkest minerals (quartz which is abundant).

I thought before I re-invent the wheel and get involved with the math and all the other possible variables (e.g., pixel resolution, irregular perimeters, ...) that I'd ask my peers if this has been done before(?) ... leastwise, I haven't yet found a reference ...

TIA & Cheerios from the Avalon
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Michael Shaffer
SEM-MLA Research Coordinator
Bruneau Centre for Research & Innovation
Memorial University
St. John's, Newfoundland, Canada
Information: http://www.mun.ca/creait/maf/
Scheduling: http://www.mun.ca/creait/maf/SEM-MLA/calendar.php
(709) 864-6799 (Ofc)
(709) 864-6790 (Lab)
cogito ergo ZzoooomM


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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 18 Jan 2012 17:37:02 -0600
Subject: [Microscopy] viaWWW:manual for a LKB 2178 Knifemaker

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Email: amy.albin-at-utoledo.edu Name: Amy Albin

Organization: University of Toledo

Title-Subject: [Filtered] LKB 2178 Knifemaker II

Message: I was wondering if anyone knew where to find a manual for a LKB 2178 Knifemaker II? So far,
google hasnt been all that helpful.
Thanks!

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From: W.Muss-at-salk.at
Date: Thu, 19 Jan 2012 02:55:40 -0600
Subject: [Microscopy] Re: manual for a LKB 2178 Knifemaker

Contents Retrieved from Microscopy Listserver Archives
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==============================================================================================================
Good Morning,

Dear Amy,
just to let you know:
The whole LKB-product branch has been taken over some 10 years ago by LEICA (cf. http://www.leica-microsystems.com/).
So you'll find some results if you search "Google" by the phrase
} LEICA 2178 KnifeMaker II { or } LEICA 2178 KnifeMaker II AND Manual {.

Since I have not found the Type you were asking for in my own PC-files (Reichert, LKB, LEICA, ZEISS, etc.)
I googled in my own interest and therefore I - exceptionally and hopefully tolerated by the listers - communicate those results I found quickly (and perhaps could be a short way out of your dilemma):

Within the first 20 results there were no results for a {MANUAL available}

but, perhaps you could be supported by EM or Imaging Lab's posessing such a KnifeMaker Type:
cf: http://www.memphis.edu/imc/services.htm a Lab which is - unfortunately - some 500 miles away from your location:

Staff communication
http://www.memphis.edu/imc/staff.htm :
Dr. Omar Skalli
Director
oskalli-at-memphis.edu
Phone: (901) 678-2034
Office: 101 Life Science Bldg

Further information on use of the IMC, including fee schedules, can be obtained by contacting Ms. Lou G. Boykins, Laboratory Coordinator at (901) 678-4233 (phone); (901) 678-4457 (FAX) or lgboykns-at-memphis.edu
Ms. Lou Boykins
Laboratory Coordinator
Phone: (901) 678-2034
Office: 101 Life Science Bldg

Ms. Renada Scott
Research Technician II
rjscott3-at-memphis.edu
Phone: (901) 678-2034
Office: 101 Life Science Bldg
Perhaps they can send you a copy of their manual.

At the moment of writing this I do not know about a positive response from any Listserver member.

Another source would be perhaps:

Material Testing - Page 5 - Manuals - HiTechTrader.com
see: http://www.hitechtrader.com/dspManuals.cfm?1=1&catID=2262&MaxRows=25&sorder=ASC&sort=m.Make&page=5

They are listing:
"This instruction manual describes the function and operation of the LKB 2178 KnifeMaker II.
LKB 2178 KnifeMaker II Instruction Manual
Make: LKB
Model: 2178
Reference No: M-050110204
This instruction manual describes the function and operation of the LKB 2178 KnifeMaker II
BUT: Disclaimer for all manuals: Manuals listed below are not for copy, sale and/or redistribution. The manuals listed below are used by HiTechTrader.com to repair and maintain equipment. Please contact us (== cf: http://www.hitechtrader.com/contact.cfm?PgID=115 ) if you need any assistance "

Hoping this perhaps was/is of help to you,

best regards,
Wolfgang MUSS PhD
EM-Lab
Univ. Inst. Pathology
SALZBURG-AUSTRIA

=======================================

Von: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com]
Gesendet: Donnerstag, 19. Jšnner 2012 00:40
An: MuŖ Wolfgang
Betreff: [Microscopy] manual for a LKB 2178 Knifemaker
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I was wondering if anyone knew where to find a manual for a LKB 2178 Knifemaker II?
So far, google hasnt been all that helpful.
Thanks!


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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 19 Jan 2012 20:04:51 -0600
Subject: [Microscopy] viaWWW:Knifemaker II: Manual Found

Contents Retrieved from Microscopy Listserver Archives
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Title-Subject: [Filtered] LKB 2178 Knifemaker II: Manual Found!

Message: Thank you all for your help! I have been sent a copy of the
manual I was looking for. Have a great day!
-Amy

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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 19 Jan 2012 20:05:42 -0600
Subject: [Microscopy] viaWWW:Uranyl acetate

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Email: bplowman-at-pacific.edu Name: Barbara Plowman

Organization: University of the Pacific, Arthur A. Dugoni School of
Dentistry

Title-Subject: [Filtered] Uranyl acetate

Message: I have been having a problem with getting uranyl acetate to go
into solution. I have been using the same bottle of UA for over 22
years. I have not had any problems when I was using nanopure water, but
now that I am using distilled water, I can't seem to make a 3% solution
of UAaq. Is there anything I could do to help it go into solution? I am
near the end of the bottle. Maybe I should just buy another bottle? Ot
try to get the lab to get the nanopure water working again ( I think
they need a special filter). Or would adding a little methanol help? I
am open to suggestions! Thank you.


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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 19 Jan 2012 20:06:20 -0600
Subject: [Microscopy] viaWWW:Cathodoluminescence

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Email: acamacho-at-rd.us.loreal.com Name: Alejandra Camacho

Organization: Sr Research Scientist at L'Oreal

Title-Subject: [Filtered] Cathodoluminescence

Message: Hello dear colleagues,
I'm seeking your help because I have two mineral samples with same
overall composition (according to my EDS, XRD's are also the same for
both) but have different provenance and different performance. I believe
the difference is in some trace elements but I need to confirm. I would
like to try cathodoluminescence before any exotic technique like neutron
activation or PIXE but I don't have a CL detector in house, can you
recommend any lab or institution where I could get a couple of samples
analyzed? (I apologize for introducing a bit of a commercial here but I
haven't been very succesful in finding this info)
Thanks in advance for you advice

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From: frank_karl-at-ardl.com
Date: Fri, 20 Jan 2012 06:50:55 -0600
Subject: [Microscopy] viaWWW:Cathodoluminescence

Contents Retrieved from Microscopy Listserver Archives
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Good Morning,
Let me recommend MicroTrace to you. They are north of Chicago and I believe they can help you out. Here's a link:

http://www.microtracescientific.com/

Good luck........
Frank Karl
ARDL

-----Original Message-----
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Email: acamacho-at-rd.us.loreal.com Name: Alejandra Camacho

Organization: Sr Research Scientist at L'Oreal

Title-Subject: [Filtered] Cathodoluminescence

Message: Hello dear colleagues,
I'm seeking your help because I have two mineral samples with same
overall composition (according to my EDS, XRD's are also the same for
both) but have different provenance and different performance. I believe
the difference is in some trace elements but I need to confirm. I would
like to try cathodoluminescence before any exotic technique like neutron
activation or PIXE but I don't have a CL detector in house, can you
recommend any lab or institution where I could get a couple of samples
analyzed? (I apologize for introducing a bit of a commercial here but I
haven't been very succesful in finding this info)
Thanks in advance for you advice

Login Host: 198.16.3.248
---------------------------------------------------------------------------



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This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.


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From: connellyps-at-nhlbi.nih.gov
Date: Thu, 19 Jan 2012 21:13:21 -0500
Subject: [Microscopy] viaWWW:Uranyl acetate

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Barbara,

I had the same problem making a 2% solution about 20 years ago - using old UA. As soon as I started using a new supply the UA went into solution as I thought it should.

If your UA comes in a can it is not a bad idea to store the bottle in the can between uses. I have found that this extends the useable life of the crystals until I empty the bottle.

Pat

Patricia Stranen Connelly
Research Assistant
NHLBI Electron Microscopy Core
National Institutes of Health
14 Service Road West
Bldg. 14E - Rm. 111B MSC 5570
Bethesda, MD 20892-5570
Phone 301-496-3491
FAX 301-402-0170
connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}

Opinions and experiences related are those of Pat Connelly and do not represent the NIH. This message is not confidential and can be freely shared and reproduced.

==============================================
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Message: I have been having a problem with getting uranyl acetate to go
into solution. I have been using the same bottle of UA for over 22
years. I have not had any problems when I was using nanopure water, but
now that I am using distilled water, I can't seem to make a 3% solution
of UAaq. Is there anything I could do to help it go into solution? I am
near the end of the bottle. Maybe I should just buy another bottle? Ot
try to get the lab to get the nanopure water working again ( I think
they need a special filter). Or would adding a little methanol help? I
am open to suggestions! Thank you.

Login Host: 138.9.63.239
=============================



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11, 30 -- From connellyps-at-nhlbi.nih.gov Fri Jan 20 09:00:24 2012
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From: baskin-at-bio.umass.edu
Date: Fri, 20 Jan 2012 09:27:08 -0600
Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks

Contents Retrieved from Microscopy Listserver Archives
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Hi,
I have no experiencing with aging uranyl acetate. But am
curious how time could cause a crystal to become insoluble. Many
chemicals on the lab shelf absorb water over time (they often form
cakes). This make them difficult to weigh out but I cannot think why
it would hinder solubility. Alternatively, could an oxide be forming
on the surface of the grains, an oxide might in insoluble (think
rust). If the UA powder is not super fine, I guess you could try to
grind it with a mortar and pestle to expose fresh surface, although
from a safety point of you that seems dubious (UA aerosol -- yumm!).
Anyway, I'd be interested to hear if anyone actually knows how time
leads to insolubility (of powders -- not mentality, smile).

As ever
Tobias


At 9:01 AM -0600 1/20/12, connellyps-at-nhlbi.nih.gov wrote:
}
}
} Barbara,
}
} I had the same problem making a 2% solution about 20 years ago -
} using old UA. As soon as I started using a new supply the UA went
} into solution as I thought it should.
}
} If your UA comes in a can it is not a bad idea to store the bottle
} in the can between uses. I have found that this extends the useable
} life of the crystals until I empty the bottle.
}
} Pat
}
} Patricia Stranen Connelly
} Research Assistant
} NHLBI Electron Microscopy Core
} National Institutes of Health
} 14 Service Road West
} Bldg. 14E - Rm. 111B MSC 5570
} Bethesda, MD 20892-5570
} Phone 301-496-3491
} FAX 301-402-0170
} connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
}
} Opinions and experiences related are those of Pat Connelly and do
} not represent the NIH. This message is not confidential and can be
} freely shared and reproduced.
}
}
} ---------------------------------------------------------------------------
} Email: bplowman-at-pacific.edu Name: Barbara Plowman
} Organization: University of the Pacific, Arthur A. Dugoni School of
} Dentistry
} Title-Subject: [Filtered] Uranyl acetate
}
} Message: I have been having a problem with getting uranyl acetate to go
} into solution. I have been using the same bottle of UA for over 22
} years. I have not had any problems when I was using nanopure water, but
} now that I am using distilled water, I can't seem to make a 3% solution
} of UAaq. Is there anything I could do to help it go into solution? I am
} near the end of the bottle. Maybe I should just buy another bottle? Ot
} try to get the lab to get the nanopure water working again ( I think
} they need a special filter). Or would adding a little methanol help? I
} am open to suggestions! Thank you.
}
} Login Host: 138.9.63.239
} =============================

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Professor
/_ / __ /__ \ \ \__ Biology Department
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ University of
Massachusetts
/ / ___ / \ \__/ \ ____ Amherst, MA, 01003
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

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5, 21 -- To: connellyps-at-nhlbi.nih.gov, bplowman-at-pacific.edu
5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
5, 21 -- Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks
5, 21 -- Cc: microscopy-at-microscopy.com
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==============================End of - Headers==============================




From: j.janssen-at-nki.nl
Date: Fri, 20 Jan 2012 09:48:40 -0600
Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Barbara,
I think this problem has to do with the pH of the water that is used. Probably the pH of the nanopure water was higher than the distilled water.

Groeten, Hans Janssen.

-----Original Message-----
X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu]
Sent: vrijdag 20 januari 2012 16:30
To: Hans Janssen

Hi,
I have no experiencing with aging uranyl acetate. But am
curious how time could cause a crystal to become insoluble. Many
chemicals on the lab shelf absorb water over time (they often form
cakes). This make them difficult to weigh out but I cannot think why
it would hinder solubility. Alternatively, could an oxide be forming
on the surface of the grains, an oxide might in insoluble (think
rust). If the UA powder is not super fine, I guess you could try to
grind it with a mortar and pestle to expose fresh surface, although
from a safety point of you that seems dubious (UA aerosol -- yumm!).
Anyway, I'd be interested to hear if anyone actually knows how time
leads to insolubility (of powders -- not mentality, smile).

As ever
Tobias


At 9:01 AM -0600 1/20/12, connellyps-at-nhlbi.nih.gov wrote:
}
}
} Barbara,
}
} I had the same problem making a 2% solution about 20 years ago -
} using old UA. As soon as I started using a new supply the UA went
} into solution as I thought it should.
}
} If your UA comes in a can it is not a bad idea to store the bottle
} in the can between uses. I have found that this extends the useable
} life of the crystals until I empty the bottle.
}
} Pat
}
} Patricia Stranen Connelly
} Research Assistant
} NHLBI Electron Microscopy Core
} National Institutes of Health
} 14 Service Road West
} Bldg. 14E - Rm. 111B MSC 5570
} Bethesda, MD 20892-5570
} Phone 301-496-3491
} FAX 301-402-0170
} connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
}
} Opinions and experiences related are those of Pat Connelly and do
} not represent the NIH. This message is not confidential and can be
} freely shared and reproduced.
}
}
} ---------------------------------------------------------------------------
} Email: bplowman-at-pacific.edu Name: Barbara Plowman
} Organization: University of the Pacific, Arthur A. Dugoni School of
} Dentistry
} Title-Subject: [Filtered] Uranyl acetate
}
} Message: I have been having a problem with getting uranyl acetate to go
} into solution. I have been using the same bottle of UA for over 22
} years. I have not had any problems when I was using nanopure water, but
} now that I am using distilled water, I can't seem to make a 3% solution
} of UAaq. Is there anything I could do to help it go into solution? I am
} near the end of the bottle. Maybe I should just buy another bottle? Ot
} try to get the lab to get the nanopure water working again ( I think
} they need a special filter). Or would adding a little methanol help? I
} am open to suggestions! Thank you.
}
} Login Host: 138.9.63.239
} =============================

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Professor
/_ / __ /__ \ \ \__ Biology Department
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ University of
Massachusetts
/ / ___ / \ \__/ \ ____ Amherst, MA, 01003
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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5, 21 -- Date: Fri, 20 Jan 2012 10:27:03 -0500
5, 21 -- To: connellyps-at-nhlbi.nih.gov, bplowman-at-pacific.edu
5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
5, 21 -- Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks
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==============================Original Headers==============================
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From: malcolm.haswell-at-sunderland.ac.uk
Date: Fri, 20 Jan 2012 10:17:06 -0600
Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I know that uranyl acetate is generally considered as only slowly/poorly soluble in water. I would suspect that acetate salts would decompose more readily than some other salts and so I had always just assumed that old uranyl acetate just slowly oxidised producing a less soluble mixture.

Certainly I had heard that 10+ year old uranyl acetate was less soluble than a fresh supply so I had always refused kind gifts of uranyl acetate if it was old.

I don't know if this helps much.

Malcolm

Malcolm Haswell
Imaging Suite
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk

________________________________________
X-from: j.janssen-at-nki.nl [j.janssen-at-nki.nl]
Sent: 20 January 2012 15:56
To: Malcolm Haswell

Dear Barbara,
I think this problem has to do with the pH of the water that is used. Probably the pH of the nanopure water was higher than the distilled water.

Groeten, Hans Janssen.

-----Original Message-----
X-from: baskin-at-bio.umass.edu [mailto:baskin-at-bio.umass.edu]
Sent: vrijdag 20 januari 2012 16:30
To: Hans Janssen

Hi,
I have no experiencing with aging uranyl acetate. But am
curious how time could cause a crystal to become insoluble. Many
chemicals on the lab shelf absorb water over time (they often form
cakes). This make them difficult to weigh out but I cannot think why
it would hinder solubility. Alternatively, could an oxide be forming
on the surface of the grains, an oxide might in insoluble (think
rust). If the UA powder is not super fine, I guess you could try to
grind it with a mortar and pestle to expose fresh surface, although
from a safety point of you that seems dubious (UA aerosol -- yumm!).
Anyway, I'd be interested to hear if anyone actually knows how time
leads to insolubility (of powders -- not mentality, smile).

As ever
Tobias


At 9:01 AM -0600 1/20/12, connellyps-at-nhlbi.nih.gov wrote:
}
}
} Barbara,
}
} I had the same problem making a 2% solution about 20 years ago -
} using old UA. As soon as I started using a new supply the UA went
} into solution as I thought it should.
}
} If your UA comes in a can it is not a bad idea to store the bottle
} in the can between uses. I have found that this extends the useable
} life of the crystals until I empty the bottle.
}
} Pat
}
} Patricia Stranen Connelly
} Research Assistant
} NHLBI Electron Microscopy Core
} National Institutes of Health
} 14 Service Road West
} Bldg. 14E - Rm. 111B MSC 5570
} Bethesda, MD 20892-5570
} Phone 301-496-3491
} FAX 301-402-0170
} connellyps-at-mail.nih.gov {mailto:connellyps-at-mail.nih.gov}
}
} Opinions and experiences related are those of Pat Connelly and do
} not represent the NIH. This message is not confidential and can be
} freely shared and reproduced.
}
}
} ---------------------------------------------------------------------------
} Email: bplowman-at-pacific.edu Name: Barbara Plowman
} Organization: University of the Pacific, Arthur A. Dugoni School of
} Dentistry
} Title-Subject: [Filtered] Uranyl acetate
}
} Message: I have been having a problem with getting uranyl acetate to go
} into solution. I have been using the same bottle of UA for over 22
} years. I have not had any problems when I was using nanopure water, but
} now that I am using distilled water, I can't seem to make a 3% solution
} of UAaq. Is there anything I could do to help it go into solution? I am
} near the end of the bottle. Maybe I should just buy another bottle? Ot
} try to get the lab to get the nanopure water working again ( I think
} they need a special filter). Or would adding a little methanol help? I
} am open to suggestions! Thank you.
}
} Login Host: 138.9.63.239
} =============================

--
_ ____ __ ____
/ \ / / \ / \ \ Tobias I. Baskin
/ / / / \ \ \ Professor
/_ / __ /__ \ \ \__ Biology Department
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ University of
Massachusetts
/ / ___ / \ \__/ \ ____ Amherst, MA, 01003
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243

==============================Original Headers==============================
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5, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
5, 21 -- Subject: [Microscopy] Re: viaWWW:Uranyl acetate-aging rocks
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==============================Original Headers==============================
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University of Sunderland - life-changing: see our new TV advert at
http://www.lifechangingsunderland.com or http://www.sunderland.ac.uk


==============================Original Headers==============================
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23, 35 -- To: "Microscopy MSA (Microscopy-at-microscopy.com)" {Microscopy-at-microscopy.com}
23, 35 -- Subject: RE: [Microscopy] FW: Re: viaWWW:Uranyl acetate-aging rocks
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From: frank_karl-at-ardl.com
Date: Fri, 20 Jan 2012 10:31:07 -0600
Subject: [Microscopy] B+W printer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Our color printer, which I use to print B-W copies of TEM images of carbon black, is sounding like a rusty gate. On the chance we
replace it, I'm looking for suggestions: What's going to give me the nicest B+W TEM print? Any suggestions? Money is (and when is it not?) a factor.

If you're shy, contact me directly, or not. All suggestions will be considered,

Thanks...................

Frank









Frank Karl
Microscopist
ARDL
2887 Gilchrist Road
Akron, Ohio 44305
330-794-6600



________________________________
This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.


==============================Original Headers==============================
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From: bozzola-at-siu.edu
Date: Fri, 20 Jan 2012 14:38:10 -0600
Subject: [Microscopy] Re: Uranyl acetate-aging rocks

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Reply to Hans.

I had used the same water source with both samples of UA , old and new. I know that the pH stayed the same most of the year so I do not think that the pH changed from morning to afternoon of the same day. If I remember correctly our pH usually ran in the 6.5 range. I know it was well below a pH of 7.0 any time I tested it.

Reply to Malcolm.

I had trouble getting the old UA to go into a 2% solution over a period of time. It just kept getting worse to the point that I had it mixing for up to two days (under a metal can to exclude the room lights) which had me questioning what was wrong when I knew that others made 4% UA with no difficulty. At that time I "borrowed" a gram from another lab which I knew was going into solution fine according to my EM Tech. friend. I then used my water, glass bottle, etc. the same that I had done before with my old UA. It went into solution within a short period of time so I ordered a new supply of UA from the same supplier of my old bottle and the borrowed UA and have not had a problem since.

I suggest that those who offer me gifts of old UA send the bottles out the next time they have a pick up of radioactive waste. I have tried several old supplies over the years and found none to go into solution readily.

Reply to Tobias.

When I started in EM back in 1971 my supply of UA was of coarse crystals which I was instructed to grind fine. I had a dedicated mortar and pestle for this purpose. I was very glad that when I ordered my first new bottle of UA some years later that it came as a fine, almost powder like grade of crystals. This is how my UA has come since that time.

Since it is now 2012 and I am still alive I guess I did a good job of not inhaling the dust! According to a very old edition of the Merc Index, UA was used as a snuff. Hard to believe with all the cautions on current MSDS sheets.

Pat Connelly

Opinions and experiences related are those of Pat Connelly and do
not represent the NIH. This message is not confidential and can be
freely shared and reproduced.

________________________________
X-from: {j.janssen-at-nki.nl}
Reply-To: {j.janssen-at-nki.nl}

Well, this has been an interesting discussion about what happens with
aging uranyl acetate (UAc) salts.

Although I am certainly not a chemist, I suspect several events (some
already mentioned by other contributors) are taking place to cause the
salts to become less soluble:

1. Degradation of acetate. Acetates are notoriously unstable and
break down with time. If you detect a strong smell of acetic acid
(carefully, by wafting the air above the container), then
decomposition is taking place.

2. Phytolytic decomposition. Most people keep the UAc away from the
light; however, this is not always the case. I've seen solutions
sitting on top of counters for long periods of time in clear, glass
containers. You should cover the container with aluminum foil and keep
it refrigerated (to cut down on light even more).

3. Radiolytic decomposition. UAc (even depleted, U238 Ac) is weakly
radioactive, giving off alpha, but also beta particles and gamma rays
as part of the decay process. Over time, these will degrade most
chemical compounds. In fact, ever wonder what was coating the inside
of your UAc staining vessel? That insoluble material is uranyl oxide
(JI√ć TEPL√Ě & V√ĀCLAV TUL√ćK. 1963. Formation of Peroxidic Precipitate in
the Radiolysis of Uranyl Nitrate Ketone Solutions. Nature 200:
671-672).

Now, for some "popular" trivia. Anyone ever hear of a Revigator?

That's a water vessel lined with uranium that releases radon into the
water! Back in the 1920's they were sold to fitness folks to "restore
water's lost element" and invigorate the body……… Frightening! You can
occasionally find them on EBay and I am surprised they are even
allowed to be sold.

--
John J. Bozzola, Ph.D., (Happily Retired) Professor & Director of IMAGE
Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL  62901
Phone: 618-453-3730


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From: DusevichV-at-umkc.edu
Date: Fri, 20 Jan 2012 15:40:33 -0600
Subject: [Microscopy] M&M 2012 - instructions for authors?

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Hi Listers,

It looks like I am becoming illegally blind: I cannot find instructions for authors and/or abstract template on M&M site. Recommended in Call for Papers link does not connect to the right page.

Were there any changes in template or I can use my old abstract as a template?

Thanks,

Vladimir


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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 23 Jan 2012 08:00:47 -0600
Subject: [Microscopy] viaWWW:Uranyl acetate

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Title-Subject: [Filtered] Uranyl acetate

Message: Thanks for all the information regarding uranyl acetate. I'm purchasing a new bottle of it!
Barbara

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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 23 Jan 2012 08:01:33 -0600
Subject: [Microscopy] viaWWW:Hitachi S-570 docs anyone need them?

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Title-Subject: [Filtered] Hitachi S-570 docs

Message: A kind person sent me a complete set of docs with schematics for this SEM. Now it seems
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Can someone use these docs? First come, first served. No charge. This is a huge data dump...originals.

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From: oshel1pe-at-cmich.edu
Date: Mon, 23 Jan 2012 08:09:44 -0600
Subject: [Microscopy] uneven negative staining of liposomes

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Note: I have already sent the O.P. Charles Humphrey's Alcian Blue
method (which he notes was published by Nermut in 1982).

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} Date: Mon, 23 Jan 2012 05:54:13 -0800
} From: {AssociationManagement-at-microscopy.org}
} Reply-To: Amanda {lever.amanda1-at-gmail.com}
} Subject: Ask-A-Microscopist
}
} Below is the result of your form, submitted on Monday, January 23,
} 2012 at 05:53:59 AM.
}
} realname - Amanda
} Email - lever.amanda1-at-gmail.com
} ORGANIZATION - Draper Laboratory
} EDUCATION - Graduate College
} LOCATION - Boston, MA
} SUBJECT_OF_QUESTION - Transmission Electron Microscopy
} QUESTION - I am currently using a UA negative stain on carbon grids
} to visualize and image liposomes. I have been noticing heterogeniety
} across the EM grids (some liposomes look nice and maintain the 3D
} shape while others appear flat and 2D in shape). Why might this be
} happening and are there any techniques to prevent it ?
}

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From: oshel1pe-at-cmich.edu
Date: Mon, 23 Jan 2012 08:46:47 -0600
Subject: [Microscopy] Used phase contrast microscope wanted

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Note: I have referred the O.P. to MSA's surplus equipment page, but
anything specific to what he's looking for would help him.

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} realname - Ralph Appy
} Email - r.appy-at-cox.net
} ORGANIZATION - Cabrillo Marine Aquarium
} LOCATION - San Pedro, CA, U.S.A.
} SUBJECT_OF_QUESTION - New/Refurbished Microscope
} QUESTION - I am a retired environmental scientist and rekindling my
} interest in fish parasitology at a local marine aquarium. I would
} like to find a used/refurbished phase contrast microscope with a
} drawing tube. Not sure if any members might have such a surplus
} microscope. Not having much luck on what I want on the internet. I
} know this is an unusual request. I am bonafide [...just published
} in J Parasitol 97(6):1035-1048] and not indepentently wealthy.
}
} Ralph


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From: eschumacher-at-mccrone.com
Date: Mon, 23 Jan 2012 09:54:13 -0600
Subject: [Microscopy] Short Course Announcement: TEM

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Greetings Fellow Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering its transmission electron microscopy short course March 6-8, 2012. In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further details and registration information, please follow the link below:


http://www.hookecollege.com/courses/course.asp?COURSE_ID=53


Best regards,

Elaine Schumacher

*********************************************************************
Elaine F.Schumacher
Senior Research Scientist
McCrone Associates, Inc.
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7417 (fax)
E-mail: eschumacher-at-mccrone.com
Web Site: www.mccrone.com



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From: donk-at-ardl.com
Date: Mon, 23 Jan 2012 10:41:28 -0600
Subject: [Microscopy] RE: Detection of Boron by EDX

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I have a sample of zinc borate that I've been asked to determine the atomic number ratio of boron to zinc. Has anybody had any luck in detecting boron and if so, how did you go about it? I have IXRF software using an Amray 1830 with a light element detector. Is it a matter of setting up the detector threshold so you can see below carbon or what? Any help would be greatly appreciated!

Don Kierstead
Microscopist
ARDL, Inc.
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From: wesaia-at-iastate.edu
Date: Mon, 23 Jan 2012 11:11:52 -0600
Subject: [Microscopy] RE: Detection of Boron by EDX

Contents Retrieved from Microscopy Listserver Archives
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The first thing you do is proceed with great caution. If your system can detect boron, I am sure it will spit out a number. It might even have some resemblance to the truth, but probably not.

The second thing you should probably do is start looking into the difficulty of light element analysis. You have boron and oxygen (light elements) combined with zinc (a rather heavy element). Look into the ZAF factors and see how big the absorption corrections. Ask yourself how well you know the mass absorption coefficients for those combinations. See what you can do about changing conditions (dropping voltage) to reduce the magnitude of the corrections.

The third thing you do would be to make sure your system can see the boron peak. My guess is that you are probably not optimized to detect boron. We had an old Kevex Quantum detector running on one of the early IXRF systems. You might be able to adjust your discriminators to better see the peak. We had to. In the end, we could see most of the boron peak, but not much if any of the background on the low energy side.

The fourth thing would be to get some reference materials. If your system cannot give you right answers on a known compound (and it probably won't), then you can forget about trying to analyze an unknown compound. You may be able to collect your own standards to help the matter, but that is not a job for novices. (If you succeed, you may have progressed beyond the novice stage. There is much more to be said on this topic.)

Another thing is to consider the limitations of EDS in general. I suppose they will not have a flat, polished sample. You'll need to consider the effects of geometry. They affect results and become more significant the lighter the elements. Depending on where you hit a particle (on the side near the detector or the side away), you can probably produce any results you want.

Finally, I would get a large supply of salt so you can hand out your results with many grains. Seriously, I would try to get your client to accept qualitative results such as "The sample shows Zn, B, and O. It could be borate." This will be even more convincing if you can compare spectra of their unknown to a known sample of zinc borate and say, "See, they match". That may be as far as you really want to push it.

Regards,
Warren Straszheim

-----Original Message-----
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Sent: Monday, January 23, 2012 10:42 AM
To: wesaia-at-iastate.edu

I have a sample of zinc borate that I've been asked to determine the atomic number ratio of boron to zinc. Has anybody had any luck in detecting boron and if so, how did you go about it? I have IXRF software using an Amray 1830 with a light element detector. Is it a matter of setting up the detector threshold so you can see below carbon or what? Any help would be greatly appreciated!

Don Kierstead
Microscopist
ARDL, Inc.
www.ardl.com
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From: FMonson-at-wcupa.edu
Date: Mon, 23 Jan 2012 15:07:05 -0600
Subject: [Microscopy] uneven negative staining of liposomes

Contents Retrieved from Microscopy Listserver Archives
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I believe I would first consider surface tension as the culprit. Are all liposomes identically resistant to the passage of the air-water interface?

Hope this at least serves as a last resort,

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl

If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state.
1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008.
2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224

-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Monday, January 23, 2012 9:18 AM
To: Monson, Frederick

Note: I have already sent the O.P. Charles Humphrey's Alcian Blue method (which he notes was published by Nermut in 1982).

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} Date: Mon, 23 Jan 2012 05:54:13 -0800
} From: {AssociationManagement-at-microscopy.org}
} Reply-To: Amanda {lever.amanda1-at-gmail.com}
} Subject: Ask-A-Microscopist
}
} Below is the result of your form, submitted on Monday, January 23,
} 2012 at 05:53:59 AM.
}
} realname - Amanda
} Email - lever.amanda1-at-gmail.com
} ORGANIZATION - Draper Laboratory
} EDUCATION - Graduate College
} LOCATION - Boston, MA
} SUBJECT_OF_QUESTION - Transmission Electron Microscopy QUESTION - I am
} currently using a UA negative stain on carbon grids to visualize and
} image liposomes. I have been noticing heterogeniety across the EM grids
} (some liposomes look nice and maintain the 3D shape while others appear
} flat and 2D in shape). Why might this be happening and are there any
} techniques to prevent it ?
}

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From: bryan.todd.hansen-at-gmail.com
Date: Mon, 23 Jan 2012 15:26:48 -0600
Subject: [Microscopy] Platinum Blue Recipe

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

I've found a couple of papers that list Pt-blue as an alternative to Uranyl
Acetate, but I haven't been able to track down the way to make it.  Does
anyone have a working recipe as how to make the Pt-blue?  Thanks for any
help/tips.

--
Bryan Hansen
EM Technician
Rocky Mountain Laboratory
NIAID/NIH
bryan.todd.hansen-at-gmail.com
hansenbry-at-niaid.nih.gov
406-363-9202


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From: john.mitchels-at-gmail.com
Date: Mon, 23 Jan 2012 15:59:16 -0600
Subject: [Microscopy] EMs in the Movies

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Hi Listers

I have for a long time been keen to find references to EMs in movies and
put them on a website for all to see. Every time I think about it I
promptly forget and the cycle repeats not this time!

I wondered is anyone has spotted EMs in movies/tv shows/documentaries?
If so what and where? If you can point out the make, model and any
inaccuracies all the better!

So far the ones I can remember are:

1. Flash Gordon 1936 (Controls of the ship are a TEM)
2. Independence Day in Area 51 scene
3. The Man in White Suit
4. Predator 2
5. CSI had a Hitachi S3400 once I recall
6. There was a fake one in Spiderman 2
7. Blade Runner

Any more would be greatly appreciated including those not in English!

Regards
John


.

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From: kenconverse-at-qualityimages.biz
Date: Mon, 23 Jan 2012 16:28:03 -0600
Subject: [Microscopy] EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Quincy. In a big voice, "Let's put it in the scanning electron microscope."
I was told that the consultant was an ETEC customer. I always thought it
was great because I knew what Quincy was talking about (even if he didn't).

Ken Converse
owner

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME 04009
207-647-4348
kenconverse-at-qualityimages.biz
qualityimages.biz


-----Original Message-----
X-from: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com]
Sent: Monday, January 23, 2012 5:01 PM
To: kenconverse-at-qualityimages.biz

Hi Listers

I have for a long time been keen to find references to EMs in movies and
put them on a website for all to see. Every time I think about it I
promptly forget and the cycle repeats not this time!

I wondered is anyone has spotted EMs in movies/tv shows/documentaries?
If so what and where? If you can point out the make, model and any
inaccuracies all the better!

So far the ones I can remember are:

1. Flash Gordon 1936 (Controls of the ship are a TEM)
2. Independence Day in Area 51 scene
3. The Man in White Suit
4. Predator 2
5. CSI had a Hitachi S3400 once I recall
6. There was a fake one in Spiderman 2
7. Blade Runner

Any more would be greatly appreciated including those not in English!

Regards
John


.

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From: john.mitchels-at-gmail.com
Date: Mon, 23 Jan 2012 16:58:38 -0600
Subject: [Microscopy] Updated Movie List

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

So far thanks to over 60 responses (and a quick trawl of the archives),
this is great keep them coming!

We are up to the following:

1. Flash Gordon
2. Independence Day
3. The Man in White Suit
4. Predator 2
5. CSI
6. Spiderman 2
7. Blade Runner
8. NCIS
9. Dexter
10.Contagion
11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008
tv-miniseries.
12. Quincy
13. X-Files
14.Pres Obama at Intel's Titan
15.Spiderman 1
16.American Pickers
17.The Last Mimzy
18 ALTO MEDIA 'travel' into the matter.
19. special effects for one of the Star Trek
20. Batman
21. Jurassic Park
22. The Bone Collector" analyzes something
23. Avatar
24. The Prisoner (British TV series)
25. The Naked Gun


Thanks for all the responses so far! Once collected I will put together
a website and send the link to the list.
John

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From: andrew.ornelas-at-intertek.com
Date: Mon, 23 Jan 2012 17:53:44 -0600
Subject: [Microscopy] Leo 1550 help

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello out there,

We have a Leo 1550 FEG-SEM that isn't running as well as it should be. We're not quite sure what the problem is exactly, but we know what the results are.

A few things are not fully functioning as our beam current is on average in the low 40 micro-amp range. Another issue is the alignment of the beam to the aperatures. When we select different apertures, the beam is supposed to be calibrated to the exact coordinates of the aperture and be deflected through the one selected. This is not happening though, instead we get a beam that moves slightly off from where it was originally, which in our case seems to be at the 30um aperture.

Does anyone out there have any experience to these columns and know of any kind of solution to these problems? I'm also looking for some sort of manual or user guide to the SEM, hopefully in pdf form, if anyone is willing to share.

Thank you for your time,


Andrew Ornelas†
Metallurgical Lab Technician
Intertek APTECH
601 West California Avenue†
Sunnyvale, CA 94086††

Tel. (Direct):†(408) 636-5308†
Tel. (Main):†(408) 745-7000†
Fax:†(408) 734-0445†
E-mail:†andrew.ornelas-at-intertek.com


Valued Quality. Delivered.
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From: donovan-at-uoregon.edu
Date: Mon, 23 Jan 2012 17:57:23 -0600
Subject: [Microscopy] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Is "The Man In The White Suit" the earliest scene with an EM in
movies that anyone knows of?

Great movie by the way.
john

At 02:01 PM 1/23/2012, you wrote:



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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 23 Jan 2012 18:55:37 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW:One more manual MT6000-XL RMC

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This Question/Comment was submitted to the Microscopy Listserver
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Title-Subject: [Filtered] One more manual...

Message: Everyone was so helpful when I was searching for a manual last time, I thought I might try
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From: allardlfjr-at-ornl.gov
Date: Mon, 23 Jan 2012 21:47:35 -0600
Subject: [Microscopy] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I agree with everything Warren says, the average EDS/EDX system is not set up for boron analysis out of the box. You need to set it up yourself, and then test with known reference materials.

I believe the best way is with WDS EPMA (probe) analysis. Most probes have special diffracting crystals optimised for the boron x-ray.

cheers,
Ron
________________________________________
X-from: wesaia-at-iastate.edu [wesaia-at-iastate.edu]
Sent: Tuesday, 24 January 2012 3:20 AM
To: Ron Rasch

The first thing you do is proceed with great caution. If your system can detect boron, I am sure it will spit out a number. It might even have some resemblance to the truth, but probably not.

The second thing you should probably do is start looking into the difficulty of light element analysis. You have boron and oxygen (light elements) combined with zinc (a rather heavy element). Look into the ZAF factors and see how big the absorption corrections. Ask yourself how well you know the mass absorption coefficients for those combinations. See what you can do about changing conditions (dropping voltage) to reduce the magnitude of the corrections.

The third thing you do would be to make sure your system can see the boron peak. My guess is that you are probably not optimized to detect boron. We had an old Kevex Quantum detector running on one of the early IXRF systems. You might be able to adjust your discriminators to better see the peak. We had to. In the end, we could see most of the boron peak, but not much if any of the background on the low energy side.

The fourth thing would be to get some reference materials. If your system cannot give you right answers on a known compound (and it probably won't), then you can forget about trying to analyze an unknown compound. You may be able to collect your own standards to help the matter, but that is not a job for novices. (If you succeed, you may have progressed beyond the novice stage. There is much more to be said on this topic.)

Another thing is to consider the limitations of EDS in general. I suppose they will not have a flat, polished sample. You'll need to consider the effects of geometry. They affect results and become more significant the lighter the elements. Depending on where you hit a particle (on the side near the detector or the side away), you can probably produce any results you want.

Finally, I would get a large supply of salt so you can hand out your results with many grains. Seriously, I would try to get your client to accept qualitative results such as "The sample shows Zn, B, and O. It could be borate." This will be even more convincing if you can compare spectra of their unknown to a known sample of zinc borate and say, "See, they match". That may be as far as you really want to push it.

Regards,
Warren Straszheim

-----Original Message-----
X-from: donk-at-ardl.com [mailto:donk-at-ardl.com]
Sent: Monday, January 23, 2012 10:42 AM
To: wesaia-at-iastate.edu

"The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in time to get the Forgflo name on a TEM in that movie. The movie was great, but of course the depiction of the use of the TEM was pretty ridiculous. Curiously, a few years ago I was chatting with a woman on a flight out of Tokyo Narita airport, while we were getting settled in the business cabin. When I mentioned working with electron microscopes, she said that her father, a long time ago, owned a company that for a short while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the microscope in The Andromeda Strain"... :-). I've never personally seen a microscope with the Forgflo logo on it...I wonder if anyone on the listserver has any familiarity with the name...?

Also, "Avatar" has a giant machine in the early part of the movie that I believe is supposed to be an electron microscope...check it out.

Larry

Dr. Lawrence F. Allard, FMSA
Distinguished Research Staff Member
High Temperature Materials Laboratory
Microscopy Group
Materials Science and Technology Division
Oak Ridge National Laboratory
1 Bethel Valley Road
PO Box 2008
Oak Ridge, TN 37831-6064
(note: the last 4 lines are sufficient for mailing or overnight courier service)
865-607-1144 (cell)
865-576-5413 (fax)
allardLFjr-at-ornl.gov

-----Original Message-----
X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu]
Sent: Monday, January 23, 2012 6:58 PM
To: Allard Jr, Lawrence Frederick

Is "The Man In The White Suit" the earliest scene with an EM in
movies that anyone knows of?

Great movie by the way.
john

At 02:01 PM 1/23/2012, you wrote:



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From: W.Muss-at-salk.at
Date: Tue, 24 Jan 2012 03:16:37 -0600
Subject: [Microscopy] Re: Platinum Blue Recipe

Contents Retrieved from Microscopy Listserver Archives
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Re to Bryan Hansen,

Hi All too,

this just to try to figure out whether you would like to get not only references but (if you like) some .pdf
on the making and the method used for staining.

Interesting method, but not the least for a substitution of uranyl-acetate (see below)

e.g. Abstract 1993:
27-V-1030 Enhancement of the BSE signal from hydrous SEM samples by use of a platinum blue.
Keiichi Tanaka and Kenji Inagaki
Seirei Christopher Coll. Nurs. ,Hamamatsu, 433 Japan.
The most serious problem for observing hydrous biological samples is deterioration of signal-noise ratio in consequence of lowering of vacuum in specimen chambers. Although metal coating is usually used for increasing SE and BSE yields on ordinary SEM studies, the method cannot be applied for wet samples. Instead of the metal coating method, therefore, we newly devised a staining method with a 'platinum blue'. Platinum blue is a general term of polymeric compounds of deep blue in which platinum [is?] coordinated to amide
groups. In this study, we used a platinum blue prepared from the reaction of cis-dichlorodiammineplatinum (II) with thymidine, being expected to have a molecular formula [Pt4 (NHx)x . (CxHxxOx) 4] +x.

{Note added by W. Muss: unfortunately the term [Pt4 (NHx)x . (CxHxxOx) 4] +x is printed so small (poor quality) that identification of Atom-numbers is not possible from this specific reprint, but from the paper out of 2007 - see below - it is ([Pt4(NH3)8(C6H13O5)4]^+5) pH = 3-4. }

This compound did not crystallize and the Pt particles were not recognized on specimen surfaces at the observation of 100.000X. The staining method was as follows. The specimens were dipped in the
concentrated solution for about 15-30 min and rinsed in distilled water. After removing excess fluid, they are immediately observed in a SEM for wet samples.
This method was very effective for enhancing SE and BSE yields, consequently, we could get enough signals for obtaining specimen images even at the vacuum of a 4.0 Torr.

or: Paper from 2007
"Paper introduces an aqueous solution of Platinum Blue (Pt-Blue) as an alternative to Uranyl actetate (UA) for staining in Transmission EM.....

There is also a paper from 2009..

On 7th of Dec. 2011 I posted the following in TOPICS/ResearchGate
(==} { http://www.researchgate.net/topic/Electron_Microscopy/ } ) or - better, since directly -

{ http://www.researchgate.net/topic/Electron_Microscopy/post/Edition_of_post_12-01-23_It_is_already_known_for_years_that_eg_lanthanum_nitrate_as_a_reagent_either_added_to_the_fixative_or_as_separate_incubation_solution_cave_specific_recipes_to_be_followed_has_s }

Header: (TEM): Substitute reagents for Uranyl-Acetate in positive and negative staining of resin ultrathin sections.

{Dear Colleagues,
I would like to inform you of an article which recently was published in Journal of Electron Microscopy (Toyko) which I found to be perhaps of interest also to you Electron Microscopists:

Masamichi Nakakoshi, Hideo Nishioka, and Eisaku Katayama
New versatile staining reagents for biological transmission electron microscopy that substitute for uranyl acetate}

Just only citing/referencing......not yet working with Platinum Blue,
but trying to play with the Lanthanides Samarium- & or Gadolinium-Triacetate-opportunity

Please tell me, whether you will accept sending of pdf's to your mail account or not...

Best regards,

Wolfgang MUSS
Member of MSA
SALZBURG, Austria




} -----UrsprŁngliche Nachricht-----
} Von: bryan.todd.hansen-at-gmail.com [mailto:bryan.todd.hansen-at-gmail.com]
} Gesendet: Montag, 23. Jšnner 2012 22:29
} An: MuŖ Wolfgang
} Betreff: [Microscopy] Platinum Blue Recipe
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} Hi All,
}
} I've found a couple of papers that list Pt-blue as an alternative to
} Uranyl
} Acetate, but I haven't been able to track down the way to make it.
}  Does
} anyone have a working recipe as how to make the Pt-blue?  Thanks for
} any
} help/tips.
}
} --
} Bryan Hansen
} EM Technician
} Rocky Mountain Laboratory
} NIAID/NIH
} bryan.todd.hansen-at-gmail.com
} hansenbry-at-niaid.nih.gov
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 24 Jan 2012 07:51:01 -0600
Subject: [Microscopy] EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I was watching the second episode of the first season of the english show
sherlock last night, Sherlock used a Leica dissecting microscope to get
some lovely scanning EM images of pollen which were characterized as a
pathogenä

Simon

Simon C. Watkins PhD
Professor, Cell Biology
Professor, Immunology
Vice Chair Cell Biology
Director Center for Biologic Imaging
University of Pittsburgh
BSTS 225

3500 Terrace St
Pittsburgh PA 15261
412-352-2277
Www.cbi.pitt.edu






On 1/23/12 10:51 PM, "allardlfjr-at-ornl.gov" {allardlfjr-at-ornl.gov} wrote:

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From: mcauliff-at-umdnj.edu
Date: Tue, 24 Jan 2012 12:23:24 -0600
Subject: [Microscopy] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Forgflo EM in The Andromeda Strain was a 4C model. Just as I was
finishing my Master's at Cal State Long Beach the Biology dept. bought
one. Our service engineer was the one who set up the EM for the movie.

Geoff

On 1/23/2012 10:48 PM, allardlfjr-at-ornl.gov wrote:
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} "The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in time to get the Forgflo name on a TEM in that movie. The movie was great, but of course the depiction of the use of the TEM was pretty ridiculous. Curiously, a few years ago I was chatting with a woman on a flight out of Tokyo Narita airport, while we were getting settled in the business cabin. When I mentioned working with electron microscopes, she said that her father, a long time ago, owned a company that for a short while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the microscope in The Andromeda Strain"... :-). I've never personally seen a microscope with the Forgflo logo on it...I wonder if anyone on the listserver has any familiarity with the name...?
}
} Also, "Avatar" has a giant machine in the early part of the movie that I believe is supposed to be an electron microscope...check it out.
}
} Larry
}
} Dr. Lawrence F. Allard, FMSA
} Distinguished Research Staff Member
} High Temperature Materials Laboratory
} Microscopy Group
} Materials Science and Technology Division
} Oak Ridge National Laboratory
} 1 Bethel Valley Road
} PO Box 2008
} Oak Ridge, TN 37831-6064
} (note: the last 4 lines are sufficient for mailing or overnight courier service)
} 865-607-1144 (cell)
} 865-576-5413 (fax)
} allardLFjr-at-ornl.gov
}
} -----Original Message-----
} X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu]
} Sent: Monday, January 23, 2012 6:58 PM
} To: Allard Jr, Lawrence Frederick
} Subject: [Microscopy] Re: EMs in the Movies
}
}
}
}
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} Is "The Man In The White Suit" the earliest scene with an EM in
} movies that anyone knows of?
}
} Great movie by the way.
} john
}
} At 02:01 PM 1/23/2012, you wrote:
}
}
}
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} } Hi Listers
} }
} } I have for a long time been keen to find references to EMs in movies and
} } put them on a website for all to see. Every time I think about it I
} } promptly forget and the cycle repeats not this time!
} }
} } I wondered is anyone has spotted EMs in movies/tv shows/documentaries?
} } If so what and where? If you can point out the make, model and any
} } inaccuracies all the better!
} }
} } So far the ones I can remember are:
} }
} } 1. Flash Gordon 1936 (Controls of the ship are a TEM)
} } 2. Independence Day in Area 51 scene
} } 3. The Man in White Suit
} } 4. Predator 2
} } 5. CSI had a Hitachi S3400 once I recall
} } 6. There was a fake one in Spiderman 2
} } 7. Blade Runner
} }
} } Any more would be greatly appreciated including those not in English!
} }
} } Regards
} } John
} }
} }
} } .
} }
} } ==============================Original Headers==============================
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Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583
mcauliff-at-umdnj.edu
**********************************************



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From: germpore-at-sonic.net
Date: Tue, 24 Jan 2012 18:39:00 -0600
Subject: [Microscopy] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Don't know how this topic came up, but of course Blade Runner has a
scene featuring a behind-the counter SEM with no need for sample prep,
used to quickly distinguish a scale of a replicant-snake from a
replicant-fish. Another scene features an image-enhancing device that
might in retrospect be seen as a form of light-field imaging.

Peter

==============================Original Headers==============================
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 24 Jan 2012 19:01:39 -0600
Subject: [Microscopy] [Filtered] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

You can watch the BBC on iPlayer if you can get a proxy server that makes it look like you are in
the UK. We just watched the second series of Sherlock.

Sent from my iPad.
--
John Mansfield PhD Cphys MInstP
2010 Microscopy & Microanalysis Program Chair
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143 USA
Phone: (734) 936-3352 FAX (734) 763-2282 Cell. Phone: (734) 834-3913

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Please note: Electronic Mail is not secure, but should be read several times every day, and should
definitely be used for urgent or sensitive issues.

On Jan 24, 2012, at 9:00 AM, microscopylistserver-noreply-at-microscopy.com wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} I was watching the second episode of the first season of the english show
} sherlock last night, Sherlock used a Leica dissecting microscope to get
} some lovely scanning EM images of pollen which were characterized as a
} pathogenÔŅĹ
}
} Simon
}
} Simon C. Watkins PhD
} Professor, Cell Biology
} Professor, Immunology
} Vice Chair Cell Biology
} Director Center for Biologic Imaging
} University of Pittsburgh
} BSTS 225
}
} 3500 Terrace St
} Pittsburgh PA 15261
} 412-352-2277
} Www.cbi.pitt.edu
}
}
}
}
}
}
} On 1/23/12 10:51 PM, "allardlfjr-at-ornl.gov" {allardlfjr-at-ornl.gov} wrote:
}
} }
} }
} }
} } --------------------------------------------------------------------------
} } --
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe --
} } http://www.microscopy.com/MicroscopyListserver
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} }
} } "The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA
} } EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in
} } time to get the Forgflo name on a TEM in that movie. The movie was
} } great, but of course the depiction of the use of the TEM was pretty
} } ridiculous. Curiously, a few years ago I was chatting with a woman on a
} } flight out of Tokyo Narita airport, while we were getting settled in the
} } business cabin. When I mentioned working with electron microscopes, she
} } said that her father, a long time ago, owned a company that for a short
} } while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the
} } microscope in The Andromeda Strain"... :-). I've never personally seen a
} } microscope with the Forgflo logo on it...I wonder if anyone on the
} } listserver has any familiarity with the name...?
} }
} } Also, "Avatar" has a giant machine in the early part of the movie that I
} } believe is supposed to be an electron microscope...check it out.
} }
} } Larry
} }
} } Dr. Lawrence F. Allard, FMSA
} } Distinguished Research Staff Member
} } High Temperature Materials Laboratory
} } Microscopy Group
} } Materials Science and Technology Division
} } Oak Ridge National Laboratory
} } 1 Bethel Valley Road
} } PO Box 2008
} } Oak Ridge, TN 37831-6064
} } (note: the last 4 lines are sufficient for mailing or overnight courier
} } service)
} } 865-607-1144 (cell)
} } 865-576-5413 (fax)
} } allardLFjr-at-ornl.gov
} }
} } -----Original Message-----
} } X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu]
} } Sent: Monday, January 23, 2012 6:58 PM
} } To: Allard Jr, Lawrence Frederick
} } Subject: [Microscopy] Re: EMs in the Movies
} }
} }
} }
} }
} } --------------------------------------------------------------------------
} } --
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } http://www.microscopy.com/MicroscopyListserver
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} }
} } Is "The Man In The White Suit" the earliest scene with an EM in
} } movies that anyone knows of?
} }
} } Great movie by the way.
} } john
} }
} } At 02:01 PM 1/23/2012, you wrote:
} }
} }
} }
} } } -------------------------------------------------------------------------
} } } ---
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } } America
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} } }
} } } Hi Listers
} } }
} } } I have for a long time been keen to find references to EMs in movies and
} } } put them on a website for all to see. Every time I think about it I
} } } promptly forget and the cycle repeats not this time!
} } }
} } } I wondered is anyone has spotted EMs in movies/tv shows/documentaries?
} } } If so what and where? If you can point out the make, model and any
} } } inaccuracies all the better!
} } }
} } } So far the ones I can remember are:
} } }
} } } 1. Flash Gordon 1936 (Controls of the ship are a TEM)
} } } 2. Independence Day in Area 51 scene
} } } 3. The Man in White Suit
} } } 4. Predator 2
} } } 5. CSI had a Hitachi S3400 once I recall
} } } 6. There was a fake one in Spiderman 2
} } } 7. Blade Runner
} } }
} } } Any more would be greatly appreciated including those not in English!
} } }
} } } Regards
} } } John
} } }
} } }
} } } .
} } }
} } } ==============================Original
} } } Headers==============================
} } } 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012
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} } } 9, 29 -- Date: Mon, 23 Jan 2012 21:59:14 +0000
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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 24 Jan 2012 19:02:00 -0600
Subject: [Microscopy] [Filtered] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

A life science professor watched Sherlock movies. As a biological processor, he used
Light microscopes a lot. So he saw something to his profession. Like me, anything related To
electron microscopes, I would be very interested.

Zhen



Sent from my iPad

On Jan 24, 2012, at 7:13 AM, "microscopylistserver-noreply-at-microscopy.com"
{microscopylistserver-noreply-at-microscopy.com} wrote:

}
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}
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} I was watching the second episode of the first season of the english show
} sherlock last night, Sherlock used a Leica dissecting microscope to get
} some lovely scanning EM images of pollen which were characterized as a
} pathogen҆
}
} Simon
}
} Simon C. Watkins PhD
} Professor, Cell Biology
} Professor, Immunology
} Vice Chair Cell Biology
} Director Center for Biologic Imaging
} University of Pittsburgh
} BSTS 225
}
} 3500 Terrace St
} Pittsburgh PA 15261
} 412-352-2277
} Www.cbi.pitt.edu
}
}
}
}
}
}
} On 1/23/12 10:51 PM, "allardlfjr-at-ornl.gov" {allardlfjr-at-ornl.gov} wrote:
}
} }
} }
} }
} } --------------------------------------------------------------------------
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} }
} } "The Andromeda Strain (1971) featured a "Forgflo" TEM (formerly RCA
} } EMU-3). Forgflo Corp. took over the RCA EM business around 1970, just in
} } time to get the Forgflo name on a TEM in that movie. The movie was
} } great, but of course the depiction of the use of the TEM was pretty
} } ridiculous. Curiously, a few years ago I was chatting with a woman on a
} } flight out of Tokyo Narita airport, while we were getting settled in the
} } business cabin. When I mentioned working with electron microscopes, she
} } said that her father, a long time ago, owned a company that for a short
} } while sold EMs. She was amazed when I said "Oh, you mean Forgflo...the
} } microscope in The Andromeda Strain"... :-). I've never personally seen a
} } microscope with the Forgflo logo on it...I wonder if anyone on the
} } listserver has any familiarity with the name...?
} }
} } Also, "Avatar" has a giant machine in the early part of the movie that I
} } believe is supposed to be an electron microscope...check it out.
} }
} } Larry
} }
} } Dr. Lawrence F. Allard, FMSA
} } Distinguished Research Staff Member
} } High Temperature Materials Laboratory
} } Microscopy Group
} } Materials Science and Technology Division
} } Oak Ridge National Laboratory
} } 1 Bethel Valley Road
} } PO Box 2008
} } Oak Ridge, TN 37831-6064
} } (note: the last 4 lines are sufficient for mailing or overnight courier
} } service)
} } 865-607-1144 (cell)
} } 865-576-5413 (fax)
} } allardLFjr-at-ornl.gov
} }
} } -----Original Message-----
} } X-from: donovan-at-uoregon.edu [mailto:donovan-at-uoregon.edu]
} } Sent: Monday, January 23, 2012 6:58 PM
} } To: Allard Jr, Lawrence Frederick
} } Subject: [Microscopy] Re: EMs in the Movies
} }
} }
} }
} }
} } --------------------------------------------------------------------------
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} }
} } Is "The Man In The White Suit" the earliest scene with an EM in
} } movies that anyone knows of?
} }
} } Great movie by the way.
} } john
} }
} } At 02:01 PM 1/23/2012, you wrote:
} }
} }
} }
} } } -------------------------------------------------------------------------
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} } }
} } } Hi Listers
} } }
} } } I have for a long time been keen to find references to EMs in movies and
} } } put them on a website for all to see. Every time I think about it I
} } } promptly forget and the cycle repeats not this time!
} } }
} } } I wondered is anyone has spotted EMs in movies/tv shows/documentaries?
} } } If so what and where? If you can point out the make, model and any
} } } inaccuracies all the better!
} } }
} } } So far the ones I can remember are:
} } }
} } } 1. Flash Gordon 1936 (Controls of the ship are a TEM)
} } } 2. Independence Day in Area 51 scene
} } } 3. The Man in White Suit
} } } 4. Predator 2
} } } 5. CSI had a Hitachi S3400 once I recall
} } } 6. There was a fake one in Spiderman 2
} } } 7. Blade Runner
} } }
} } } Any more would be greatly appreciated including those not in English!
} } }
} } } Regards
} } } John
} } }
} } }
} } } .
} } }
} } } ==============================Original
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} } } 9, 29 -- From john.mitchels-at-gmail.com Mon Jan 23 15:59:16 2012
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} } 7, 24 -- Subject: [Filtered] Re: [Microscopy] EMs in the Movies
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} } 17, 30 -- From allardlfjr-at-ornl.gov Mon Jan 23 21:47:35 2012
} } 17, 30 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.14.62])
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} } 17, 30 -- ([160.91.2.112]) with mapi; Mon, 23 Jan 2012 22:47:35 -0500
} } 17, 30 -- From: "Allard Jr, Lawrence Frederick" {allardlfjr-at-ornl.gov}
} } 17, 30 -- To: "'donovan-at-uoregon.edu'" {donovan-at-uoregon.edu} ,
} } 17, 30 -- "'microscopy-at-microscopy.com'"
} } {microscopy-at-microscopy.com}
} } 17, 30 -- Date: Mon, 23 Jan 2012 22:47:34 -0500
} } 17, 30 -- Subject: [Filtered] RE: [Microscopy] Re: EMs in the Movies
} } 17, 30 -- Thread-Topic: [Microscopy] Re: EMs in the Movies
} } 17, 30 -- Thread-Index: AczaKtvDsddVkLzBTSmxmtMNnJbLDQAHe0MA
} } 17, 30 -- Message-ID:
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From: bozzola-at-siu.edu
Date: Tue, 24 Jan 2012 21:33:51 -0600
Subject: [Microscopy] EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Our "Almost Famous" TEM:

Several of you mentioned Blade Runner, directed by Ridley Scott.

Back in 1994, we were contacted by an assistant to Mr. Scott for lease
of our recently purchased, Hitachi H7100 TEM. They were completing
construction of a laboratory set for the film, "Crisis in The Hot
Zone," and Mr. Scott wanted a functioning TEM, as was described in the
book by Richard Preston. The film was to star Robert Redford and Jodie
Foster (who was to operate the TEM).

The production assistant contacted Hitachi to pursue the lease of a
TEM for the interior shots, but Hitachi informed them the TEM they
wanted was to be delivered to SIU that month. After speaking to the
assistant, we agreed to have the TEM delivered to the set in Los
Angeles, where HItachi technicians would install and make the scope
operational for filming. Afterwards, it would be repacked and shipped
to SIU for regular installation. We had already dubbed it, The Jodie
Foster Microscope………. sigh.

Several weeks went by and we received a FAX from the assistant
indicating that the film was being shelved due to script disagreements
and since a similar film, Outbreak, was to be released at about the
same time. I saw the awful film and was quite disappointed with the
science portrayed in the film. Outbreak was fictional; whereas, Hot
Zone is based on an actual incident. Steven King is said to have
commented that Hot Zone as the scariest thing he ever read.

Supposedly, a more recent film, "Contagion." with Gwyneth Paltrow and
Matt Damon "feels very much like the movie that should have been
adapted from the Hot Zone. Has anyone seen "Contagion"? I don't think
it did well in the box office. Any TEM's in that film?

Cheers,

--
John J. Bozzola, Ph.D., Professor & Director of IMAGE (Happily Retired :-)
Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL  62901
Phone: 618-453-3730

===========================================================

} Don't know how this topic came up, but of course Blade Runner has a
} scene featuring a behind-the counter SEM with no need for sample prep,
} used to quickly distinguish a scale of a replicant-snake from a
} replicant-fish. Another scene features an image-enhancing device that
} might in retrospect be seen as a form of light-field imaging.
}
} Peter
}
} ==============================Original


==============================Original Headers==============================
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From: PWebster-at-hei.org
Date: Wed, 25 Jan 2012 00:12:42 -0600
Subject: [Microscopy] EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear "happily retired" John,

Contagion is on the list that was sent out. It features and FEI Titan. However, I hear that the company only shipped the outer box for filming. It looks like Gwyneth Paltrow never was able to use it!

If we are looking at microscopes on screen, our FEI G2 20 was featured in a TV advert for the University of Phoenix. We had the film crew here for a day. I estimated about 45 people, all the equipment you ever would need for any sort of scene, and a whole day of filming. The shot on the screen was about 2 seconds long.

Working in Los Angels, we sometimes get good deals on used equipment. For example, we have a pink Zeiss light microscope that featured in the first Jurassic Park movie. Apparently pink objects look white on film, so the machine was painted pink.

The "biggest electron microscope on the East Coast" featured in the first "Spiderman" (and where Peter Parker was bitten by the spider) was a model constructed around a statue in the front atrium of the LA Natural History Museum. Is there anyone on the list from the museum? It would be great to get another "back-stage" look at the collection.

The SEM used by Harrison Ford in Blade Runner was supposedly located in the Los Angeles Chinatown. We guessed it took place in the future because it was raining.

Best wishes on your retirement John.

Paul.

X-from Still Sunny California
House Research Institute (we changed our name!)
2100 W 3rd St
Los Angeles
CA 90057.


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Tue 1/24/2012 7:36 PM
To: Webster, Paul

Our "Almost Famous" TEM:

Several of you mentioned Blade Runner, directed by Ridley Scott.

Back in 1994, we were contacted by an assistant to Mr. Scott for lease
of our recently purchased, Hitachi H7100 TEM. They were completing
construction of a laboratory set for the film, "Crisis in The Hot
Zone," and Mr. Scott wanted a functioning TEM, as was described in the
book by Richard Preston. The film was to star Robert Redford and Jodie
Foster (who was to operate the TEM).

The production assistant contacted Hitachi to pursue the lease of a
TEM for the interior shots, but Hitachi informed them the TEM they
wanted was to be delivered to SIU that month. After speaking to the
assistant, we agreed to have the TEM delivered to the set in Los
Angeles, where HItachi technicians would install and make the scope
operational for filming. Afterwards, it would be repacked and shipped
to SIU for regular installation. We had already dubbed it, The Jodie
Foster Microscope‚?¶‚?¶‚?¶. sigh.

Several weeks went by and we received a FAX from the assistant
indicating that the film was being shelved due to script disagreements
and since a similar film, Outbreak, was to be released at about the
same time. I saw the awful film and was quite disappointed with the
science portrayed in the film. Outbreak was fictional; whereas, Hot
Zone is based on an actual incident. Steven King is said to have
commented that Hot Zone as the scariest thing he ever read.

Supposedly, a more recent film, "Contagion." with Gwyneth Paltrow and
Matt Damon "feels very much like the movie that should have been
adapted from the Hot Zone. Has anyone seen "Contagion"? I don't think
it did well in the box office. Any TEM's in that film?

Cheers,

--
John J. Bozzola, Ph.D., Professor & Director of IMAGE (Happily Retired :-)
Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, IL  62901
Phone: 618-453-3730

===========================================================

} Don't know how this topic came up, but of course Blade Runner has a
} scene featuring a behind-the counter SEM with no need for sample prep,
} used to quickly distinguish a scale of a replicant-snake from a
} replicant-fish. Another scene features an image-enhancing device that
} might in retrospect be seen as a form of light-field imaging.
}
} Peter
}
} ==============================Original


==============================Original Headers==============================
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==============================Original Headers==============================
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 25 Jan 2012 08:46:56 -0600
Subject: [Microscopy] viaWWW:Disposal of working TEM (Microscope)

Contents Retrieved from Microscopy Listserver Archives
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Title-Subject: [Filtered] Disposal of working TEM (Microscope)

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From: David.Patton-at-uwe.ac.uk
Date: Wed, 25 Jan 2012 09:04:49 -0600
Subject: [Microscopy] Updated Movie List

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My favourite example is from the X-Files. Scully visits a lab and watches the electron microscopist use a TEM. She then gives Scully an SEM image of a pollen grain or spore and tells her it must be the alien virus. Needless to say, the electron microscopist is involved in a fatal automobile accident that evening.

Dave

-----Original Message-----
X-from: David Patton
Sent: 24 January 2012 16:50
To: 'john.mitchels-at-gmail.com'

So far thanks to over 60 responses (and a quick trawl of the archives),
this is great keep them coming!

We are up to the following:

1. Flash Gordon
2. Independence Day
3. The Man in White Suit
4. Predator 2
5. CSI
6. Spiderman 2
7. Blade Runner
8. NCIS
9. Dexter
10.Contagion
11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008
tv-miniseries.
12. Quincy
13. X-Files
14.Pres Obama at Intel's Titan
15.Spiderman 1
16.American Pickers
17.The Last Mimzy
18 ALTO MEDIA 'travel' into the matter.
19. special effects for one of the Star Trek
20. Batman
21. Jurassic Park
22. The Bone Collector" analyzes something
23. Avatar
24. The Prisoner (British TV series)
25. The Naked Gun


Thanks for all the responses so far! Once collected I will put together
a website and send the link to the list.
John

==============================Original Headers==============================
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From: W.Muss-at-salk.at
Date: Wed, 25 Jan 2012 09:46:46 -0600
Subject: [Microscopy] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear List,
dear John,

out of my files, just as a supplement to your posts:

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

There is also an orange column TEM in the TV series {Dexter} . When he's in his little lab.
I too have been bored by TV and movies.

More TEM's in movies I say.... actually how about an old VG HB501. I know the one here has more character and versatility than some leading actors and actresses out there and it works for free.
The microscopist on the other hand will need food, drink and board.
{ { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { { {


best regards and wishes,

Wolfgang MUSS
SALZBURG-AUSTRIA



----

} -----UrsprŁngliche Nachricht-----
} Von: john.mitchels-at-gmail.com [mailto:john.mitchels-at-gmail.com]
} Gesendet: Montag, 23. Jšnner 2012 23:01
} An: MuŖ Wolfgang
} Betreff: [Microscopy] EMs in the Movies
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
} Hi Listers
}
} I have for a long time been keen to find references to EMs in movies
} and put them on a website for all to see. Every time I think about it I
} promptly forget and the cycle repeats not this time!
}
} I wondered if anyone has spotted EMs in movies/tv shows/documentaries?
}
} If so what and where? If you can point out the make, model and any
} inaccuracies all the better!
}
} So far the ones I can remember are:
}
} 1. Flash Gordon 1936 (Controls of the ship are a TEM)
} 2. Independence Day in Area 51 scene
} 3. The Man in White Suit
} 4. Predator 2
} 5. CSI had a Hitachi S3400 once I recall
} 6. There was a fake one in Spiderman 2
} 7. Blade Runner
}
} Any more would be greatly appreciated including those not in English!
}
} Regards
} John
}
}
} .
}
} ==============================Original
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From: microtomy-at-gmail.com
Date: Wed, 25 Jan 2012 00:13:40 -0600
Subject: [Microscopy] EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

What I take from this story is that University of Phoenix wants to
portray that they have electron microscopes, yet they have no such
thing at any of their many campuses.

Jay Campbell

---------- Forwarded message ----------
X-from: PWebster-at-hei.org

Dear "happily retired" John,

Contagion is on the list that was sent out. It features and FEI Titan.
However, I hear that the company only shipped the outer box for
filming. It looks like Gwyneth Paltrow never was able to use it!

If we are looking at microscopes on screen, our FEI G2 20 was featured
in a TV advert for the University of Phoenix. We had the film crew
here for a day. I estimated about 45 people, all the equipment you
ever would need for any sort of scene, and a whole day of filming. The
shot on the screen was about 2 seconds long.

Working in Los Angels, we sometimes get good deals on used equipment.
For example, we have a pink Zeiss light microscope that featured in
the first Jurassic Park movie. Apparently pink objects look white on
film, so the machine was painted pink.

The "biggest electron microscope on the East Coast" featured in the
first "Spiderman" (and where Peter Parker was bitten by the spider)
was a model constructed around a statue in the front atrium of the LA
Natural History Museum. Is there anyone on the list from the museum?
It would be great to get another "back-stage" look at the collection.

The SEM used by Harrison Ford in Blade Runner was supposedly located
in the Los Angeles Chinatown. We guessed it took place in the future
because it was raining.

Best wishes on your retirement John.

Paul.

X-from Still Sunny California
House Research Institute (we changed our name!)
2100 W 3rd St
Los Angeles
CA 90057.


-----Original Message-----
X-from: bozzola-at-siu.edu [mailto:bozzola-at-siu.edu]
Sent: Tue 1/24/2012 7:36 PM
To: Webster, Paul

Our "Almost Famous" TEM:

Several of you mentioned Blade Runner, directed by Ridley Scott.

Back in 1994, we were contacted by an assistant to Mr. Scott for lease
of our recently purchased, Hitachi H7100 TEM. They were completing
construction of a laboratory set for the film, "Crisis in The Hot
Zone," and Mr. Scott wanted a functioning TEM, as was described in the
book by Richard Preston. The film was to star Robert Redford and Jodie
Foster (who was to operate the TEM).

The production assistant contacted Hitachi to pursue the lease of a
TEM for the interior shots, but Hitachi informed them the TEM they
wanted was to be delivered to SIU that month. After speaking to the
assistant, we agreed to have the TEM delivered to the set in Los
Angeles, where HItachi technicians would install and make the scope
operational for filming. Afterwards, it would be repacked and shipped
to SIU for regular installation. We had already dubbed it, The Jodie
Foster MicroscopeńĀ?¬¶ńĀ?¬¶ńĀ?¬¶. sigh.

Several weeks went by and we received a FAX from the assistant
indicating that the film was being shelved due to script disagreements
and since a similar film, Outbreak, was to be released at about the
same time. I saw the awful film and was quite disappointed with the
science portrayed in the film. Outbreak was fictional; whereas, Hot
Zone is based on an actual incident. Steven King is said to have
commented that Hot Zone as the scariest thing he ever read.

Supposedly, a more recent film, "Contagion." with Gwyneth Paltrow and
Matt Damon "feels very much like the movie that should have been
adapted from the Hot Zone. Has anyone seen "Contagion"? I don't think
it did well in the box office. Any TEM's in that film?

Cheers,

--
John J. Bozzola, Ph.D., Professor & Director of IMAGE (Happily Retired :-)
Integrated Microscopy & Graphics Expertise
Southern Illinois University
750 Communications Drive
Carbondale, ILńÄ 62901
Phone: 618-453-3730

===========================================================

} Don't know how this topic came up, but of course Blade Runner has a
} scene featuring a behind-the counter SEM with no need for sample prep,
} used to quickly distinguish a scale of a replicant-snake from a
} replicant-fish. Another scene features an image-enhancing device that
} might in retrospect be seen as a form of light-field imaging.
}
} Peter
}
} ==============================Original


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From: oshel1pe-at-cmich.edu
Date: Wed, 25 Jan 2012 10:44:22 -0600
Subject: [Microscopy] Re: Updated Movie List

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

What episode of American Pickers had an EM in it?

} So far thanks to over 60 responses (and a quick trawl of the archives),
} this is great keep them coming!
}
} We are up to the following:
}
} 1. Flash Gordon
} 2. Independence Day
} 3. The Man in White Suit
} 4. Predator 2
} 5. CSI
} 6. Spiderman 2
} 7. Blade Runner
} 8. NCIS
} 9. Dexter
} 10.Contagion
} 11. Andromeda Strain The Andromeda Strain (1971)and then the remake 2008
} tv-miniseries.
} 12. Quincy
} 13. X-Files
} 14.Pres Obama at Intel's Titan
} 15.Spiderman 1
} 16.American Pickers
} 17.The Last Mimzy
} 18 ALTO MEDIA 'travel' into the matter.
} 19. special effects for one of the Star Trek
} 20. Batman
} 21. Jurassic Park
} 22. The Bone Collector" analyzes something
} 23. Avatar
} 24. The Prisoner (British TV series)
} 25. The Naked Gun
}
}
} Thanks for all the responses so far! Once collected I will put together
} a website and send the link to the list.
} John

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: auntdaisy-at-gmail.com
Date: Wed, 25 Jan 2012 11:33:56 -0600
Subject: [Microscopy] Updated Movie List

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Doctor Who and the Hand of Fear - it's used to examine a stone hand (that
comes to life). Not sure of the make, but could be Cambridge
Instruments... I'll dig out the DVD.

Great idea,

Austin

P.S. Partial side view here

http://2.bp.blogspot.com/_ZNUdpril8U4/TJbIfCxZatI/AAAAAAAAC2k/kvYB7CMhYRU/s1
600/vlcsnap-2010-09-18-13h31m33s59.jpg

http://2.bp.blogspot.com/_ZNUdpril8U4/TJbIYub7DzI/AAAAAAAAC2c/S7HVZJAXusw/s1
600/vlcsnap-2010-09-18-13h11m01s37.jpg

http://classicalgallifrey.blogspot.com/2010/09/serial-87-hand-of-fear.html

} } -----Original Message-----
} } From: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
} } Sent: 25 January 2012 16:47
} } To: AuntDaisy-at-gmail.com
} } Subject: [SPAM] [Microscopy] Re: Updated Movie List
} }
} }
} }
} }
} } ------------------------------------------------------------------------
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} --
} } --
} }
} } What episode of American Pickers had an EM in it?
} }
} } } So far thanks to over 60 responses (and a quick trawl of the archives),
} } } this is great keep them coming!
} } }
} } } We are up to the following:
} } }
} } } 1. Flash Gordon
} } } 2. Independence Day
} } } 3. The Man in White Suit
} } } 4. Predator 2
} } } 5. CSI
} } } 6. Spiderman 2
} } } 7. Blade Runner
} } } 8. NCIS
} } } 9. Dexter
} } } 10.Contagion
} } } 11. Andromeda Strain The Andromeda Strain (1971)and then the remake
} 2008
} } } tv-miniseries.
} } } 12. Quincy
} } } 13. X-Files
} } } 14.Pres Obama at Intel's Titan
} } } 15.Spiderman 1
} } } 16.American Pickers
} } } 17.The Last Mimzy
} } } 18 ALTO MEDIA 'travel' into the matter.
} } } 19. special effects for one of the Star Trek
} } } 20. Batman
} } } 21. Jurassic Park
} } } 22. The Bone Collector" analyzes something
} } } 23. Avatar
} } } 24. The Prisoner (British TV series)
} } } 25. The Naked Gun
} } }
} } }
} } } Thanks for all the responses so far! Once collected I will put together
} } } a website and send the link to the list.
} } } John
} }
} } --
} } Philip Oshel
} } Microscopy Facility Supervisor
} } Biology Department
} } 024C Brooks Hall
} } Central Michigan University
} } Mt. Pleasant, MI 48859
} } (989) 774-3576
} }
} } ==============================Original
} } Headers==============================
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} } 3, 26 -- Date: Wed, 25 Jan 2012 11:44:19 -0500
} } 3, 26 -- To: {Microscopy-at-microscopy.com}
} } 3, 26 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
} } 3, 26 -- Subject: Re: [Microscopy] Updated Movie List
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 25 Jan 2012 21:18:17 -0600
Subject: [Microscopy] viaWWW:Sodium Cacodylet buffer

Contents Retrieved from Microscopy Listserver Archives
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Email: reganhll-at-gmail.com Name: Johnson

Organization: FMLS

Title-Subject: [Filtered] EM

Message: Dear Listserv members,
It would be nice to know what are the advantages of Sodium Cacodylet buffer over other buffer. I
looked for literature but most of the books and documents speak about the safety aspects.
Greetings,
Johnson.



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From: nizets2-at-yahoo.com
Date: Thu, 26 Jan 2012 01:32:37 -0600
Subject: [Microscopy] viaWWW:Sodium Cacodylet buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Johnson!

The use of cacodylate as a buffer for EM is more a question of conservationism than anything else.
We don't use it because it is the best, we use it because it has been used successfully for more than 50 years and we are too lazy to try something else.
Some buffers are better for specific applications, but cacodylate works most of the time for a large panel of samples.
Contrary to phosphate buffers, cacodylate does not precipitate in presence of calcium. This is important because it has been shown that calcium stabilizes somes structures during fixation. Tris is not an option because it has an amine and that is what aldehydes react with.
Hepes is really a possible alternative, it is a strong buffer and it is compatible with calcium.
Personally I go with Hepes and I still have to see a bad fixation due to the buffer (which doesn't mean it is best for all samples of course).

Cheers,
Stephane



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Email: reganhll-at-gmail.com Name: Johnson

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Title-Subject: [Filtered] EM

Message: Dear Listserv members,
It would be nice to know what are the advantages of Sodium Cacodylet buffer over other buffer. I
looked for literature but most of the books and documents speak about the safety aspects.
Greetings,
Johnson.



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From: nizets2-at-yahoo.com
Date: Thu, 26 Jan 2012 04:48:36 -0600
Subject: [Microscopy] which computer parts to store?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello!
I am responsible for a FEI Tecnai G20 which was installed in 2005.
As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?).
I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time.
I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store?
Many thanks in advance.

Stephane

==============================Original Headers==============================
2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012
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2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com}
2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
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From: bozzola-at-siu.edu
Date: Thu, 26 Jan 2012 05:39:04 -0600
Subject: [Microscopy] Re: which computer parts to store?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Computers are fairly inexpensive (compared to the equipment they run).
I would have at least one complete computer standing by in the event
of failure.

In my experience, hard drives are the first to go, so back up your
hard drives from time to time and have a spare main HD ready to
replace the one in the computer.

John Bozzola

On Thu, Jan 26, 2012 at 4:49 AM, {nizets2-at-yahoo.com} wrote:
}
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} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Hello!
} I am responsible for a FEI Tecnai G20 which was installed in 2005.
} As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?).
} I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time.
} I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store?
} Many thanks in advance.
}
} Stephane
}
} ==============================Original Headers==============================
} 2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012
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} 2, 34 -- Date: Thu, 26 Jan 2012 02:48:35 -0800 (PST)
} 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
} 2, 34 -- Subject: which computer parts to store?
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--
John J. Bozzola, Ph.D., Retired
Professor & Director of IMAGE
Integrated Microscopy & Graphics Expertise
Southern Illinois University
Carbondale, IL  62901


==============================Original Headers==============================
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8, 20 -- Subject: Re: [Microscopy] which computer parts to store?
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From: malcolm.haswell-at-sunderland.ac.uk
Date: Thu, 26 Jan 2012 06:38:37 -0600
Subject: [Microscopy] Re: which computer parts to store?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have just upgraded hardware computer links and the Windows operating system on a Hitachi S3000N SEM.

The problems will not be same for an FEI except that any interface units used in either the Hitachi or FEI will have been built to a particular PC standard and these change. In terms of maintaining PC hardware therefore it would be worth looking into exactly what FEI boards will link with on a PC interface as well as any limitations dictated by operating systems. It may for instance only be feasible to link FEI boards with an XP system. But the real killer will be the interface which for internal boards was ISA, became EISA and is generally PCI now where even external systems have changed from RS232, various parallel ports, USB (1,2 & 3) and Firewire.

What I'm saying is that you need to know what is the most recent type of PC, operating system and interface that is compatible with the FEI boards and make sure you have a fully functioning system. It's surprising how quickly standards change in PCs and no amount of emulators and patched systems may be able to rescue you when all the old systems have gone.

Incidentally our S3000N now runs on Windows XP which is a vaste improvement on the old Windows NT4.0 system but it's unlikely that we will ever be able to update/upgrade further. Anyway good luck.

Malcolm

Malcolm Haswell
Imaging Suite
Faculty of Applied Sciences
University of Sunderland
SUNDERLAND
SR1 3SD
UK
email: malcolm.haswell-at-sunderland.ac.uk


________________________________________
X-from: bozzola-at-siu.edu [bozzola-at-siu.edu]
Sent: 26 January 2012 11:47
To: Malcolm Haswell

Computers are fairly inexpensive (compared to the equipment they run).
I would have at least one complete computer standing by in the event
of failure.

In my experience, hard drives are the first to go, so back up your
hard drives from time to time and have a spare main HD ready to
replace the one in the computer.

John Bozzola

On Thu, Jan 26, 2012 at 4:49 AM, {nizets2-at-yahoo.com} wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello!
} I am responsible for a FEI Tecnai G20 which was installed in 2005.
} As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?).
} I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time.
} I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store?
} Many thanks in advance.
}
} Stephane
}
} ==============================Original Headers==============================
} 2, 34 -- From nizets2-at-yahoo.com Thu Jan 26 04:48:36 2012
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} 2, 34 -- Date: Thu, 26 Jan 2012 02:48:35 -0800 (PST)
} 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 2, 34 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
} 2, 34 -- Subject: which computer parts to store?
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--
John J. Bozzola, Ph.D., Retired
Professor & Director of IMAGE
Integrated Microscopy & Graphics Expertise
Southern Illinois University
Carbondale, IL 62901


==============================Original Headers==============================
8, 20 -- From bozzola-at-siu.edu Thu Jan 26 05:39:04 2012
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From: jehrman-at-mta.ca
Date: Thu, 26 Jan 2012 07:12:16 -0600
Subject: [Microscopy] Re: which computer parts to store?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In my experience with various instruments (microscopes and otherwise),
host computer parts most likely to fail (in rough order of likelihood) are:

1. Hard drives
2. Power supplies
3. Motherboards

As the computer ages, all these parts can be had for next to nothing, if
you keep your eye out for them on eBay, second hand shops, and even the
dumpster. Having a pile of working hard drives that can replace the
working one is a great comfort. Likewise power supplies and
motherboards, although I'm lucky enough to have a local repair shop that
has been able to repair both in several cases for a very reasonable
price. Most often mobos fail either because the CMOS battery dies, or
some of the capacitors fail, which is often indicated by a swollen
appearance, particularly at the top. These are relatively easy to
replace for someone who is handy with a soldering iron. Power supplies
are also easily and inexpensively repaired by those who know how.

Having just done this yesterday, the longevity of a computer can be
extended by a yearly cleaning of the inside of the computer, esp. fans
and heatsinks. I've seen some neglected computers that were *completely*
full of dust bunnies and running hotter than a two dollar pistol. Not
good for any of the components! I believe regular cleaning is one reason
that I'm still running an HP Vectra computer on our EDS system that just
had its 12th birthday (knock wood).

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Intaxication: Euphoria at getting a
tax refund, which lasts until you realize
that it was your money to start with.

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From: oshel1pe-at-cmich.edu
Date: Thu, 26 Jan 2012 07:39:14 -0600
Subject: [Microscopy] Re: viaWWW:Sodium Cacodylet buffer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Generally, sodium cacodylate seems to extract less cytoplasm than
phosphate buffers. Advantage is more cytoplasm, disadvantage is less
contrast because there is more cytoplasm. There may well be (likely
are) specific procedures or specimens where cacodylate buffer is
better.
The arsenic content is likely more important.
You might want to have a look at "Biomedical Electron Microscopy" by
Maunsbach and Afzelius - they show many EMs comparing different
preparatory methods (fixatives, buffers, etc.), embedding resins, and
so on. A good book to have around.

Phil

} Message: Dear Listserv members,
} It would be nice to know what are the advantages of Sodium Cacodylet
} buffer over other buffer. I
} looked for literature but most of the books and documents speak
} about the safety aspects.
} Greetings,
} Johnson.

--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

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From: vray-at-partbeamsystech.com
Date: Thu, 26 Jan 2012 07:39:38 -0600
Subject: [Microscopy] Re: which computer parts to store?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephane,

As others already suggested, first priority is to have:

1) Harddrive with "carbon copy" of currently running software - it is
very easy to create with DD (Disk Dump) utility;
2) Motherboard;
3) Processor;

But do not assume that interface cards in the PC are made by OEM of the
TEM. I am not familiar with Technai specifically, but chances are that
most (if not all) cards installed in the control computer are
off-the-shelf products. If such adapted card becomes unavailable from
its OEM, it will becom impossible (or next to impossible) to get from
the FEI. So by all means solicit help from someone who is very familiar
with PC hardware and get all the cards sitting on PC bus.

Cheers :)
Valery Ray
=================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844 USA
Phone: +1-978-296-5063 - leave a message with call-back number
US Mobile: +1-978-305-0479
Skype: pbstmeo
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 1/26/2012 5:49 AM, nizets2-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
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}
} Hello!
} I am responsible for a FEI Tecnai G20 which was installed in 2005.
} As we discussed several times in the list, it is advised to store computer parts for future replacement needs because the computers evolve a lot during the lifetime of a TEM (or was it meant to buy a complete second PC?).
} I suppose that the interface cards come from FEI themselves, so they will probably be able to provide them for some time.
} I am not really a genius in computer matters and I would need you opinion about which hardware part of the computer we need to store?
} Many thanks in advance.
}
} Stephane
}
} ==============================Original Headers==============================
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} 2, 34 -- From: Stephane Nizet {nizets2-at-yahoo.com}
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From: jrminter-at-rochester.rr.com
Date: Thu, 26 Jan 2012 08:38:46 -0600
Subject: [Microscopy] Re: which computer parts to store?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stephane
As John Bozzola wisely recommends, have at least one complete computer
standing by with a ghost HD. Don't be sure that company will keep any spare
cards for a long time. I have no experience with FEI but JEOL was unable to
provide a spare graphic card just 7 years after the model (JSM5600LV)
appeared. Make sure your spare computer is in a working condition by putting
it on from time to time. This frenzy of consumarism wlll have no end and we
have to get prepared for the worse..
Best -yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
eikonika-at-otenet.gr
www.aim.cat
***********************************


----- Original Message -----
X-from: {nizets2-at-yahoo.com}
To: {eikonika-at-otenet.gr}
Sent: Thursday, January 26, 2012 12:54 PM

Stephane raised a great question and there have been several great
suggestions. We have a lab full of older FEI SEM and TEM microscopes
and have discussed this with our field service engineer. Our engineer
tells us that FEI learned from the problems they had with the
availability of components in early computer-controlled microscopes
because so much functionality was on the microscope PC. I think FEI
was not unique in encountering this problem. The engineer told us that
FEI made a concerted effort to move as much functionality as possible
into the microscope and to use multiple fast ethernet connections to
communicate with the PC. This makes upgrading the PC easier because
fewer custom boards are used. We have an older dual-beam FIB where
component availability has been a real issue. We always get scrutiny
from our IS folks because we cannot move out of unsupported operating
systems...

I would have a frank conversation with FEI's service specialist about
where your particular microscope is on this development timeline and
what your particular exposure is to part availability. At a minimum, I
would always have a spare hard drive with a disk image of a working
system configured the way you want sitting in a sealed container in
the lab. This has saved our bacon many times...

Best regards,
John Minter
Eastman Kodak

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3, 28 -- Subject: Re: [Microscopy] which computer parts to store?
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From: stefan.diller-at-t-online.de
Date: Thu, 26 Jan 2012 12:29:46 -0600
Subject: [Microscopy] Wet cryo sectioning

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Dear All,
I am just in my first experience of doing wet cryo ultramicrotome on
diamond sectioning of a PE compound containg ca. 1um size particles.
First I tried dry sectioning on a glass knife at -120įC but the cuts
came down rolled up very small in diameter. I had no idea how to smooth
them. Cutting seems impossible below 100 nm.
Wet cryo cuts now are coming off in 70% DMSO at -50įC at least somewhat
flat but very compressed. Thickness cutting is now 50 nm.
Can you give my some basic advice what I can improve (might be a lot ;-) ).

Best wishes,
Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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From: pwebster-at-hei.org
Date: Thu, 26 Jan 2012 14:09:08 -0600
Subject: [Microscopy] Quantifying immunogold label on EM thin sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Course Reminder

Quantifying immunogold label on EM thin sections

April 5th to 7th, 2012

House Research Institute, Los Angeles, CA 90057

This is a 3-day course aimed at providing instruction on how to qualify
immunogold label from images collected using transmission electron
microscopy.

During the past decade, new ways of quantifying gold labeling within cells
have been devised. Their efficiency and validity rely on sound principles of
specimen sampling, event counting and inferential statistics. These
stereological tools will be introduced in lectures as well as by using
practical examples. Participants will be provided with practical exercises
to illustrate gold counting methods, which can be taken away for future
reference.

This is an intensive, hands-on course that is organized by an expert
electron microscopist and immunologist, and taught by the developer of many
of these stereological tools, who is also an expert stereologist.

For further details contact:
Paul Webster, Ph.D.
House Research Institute
2100 West Third Street
Los Angeles
CA 90057

Or check out http://immunogold.houseresearch.org


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3, 18 -- Subject: Quantifying immunogold label on EM thin sections
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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 26 Jan 2012 18:13:14 -0600
Subject: [Microscopy] viaWWW:EVO 18 SEM from ZEISS

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Email: numan.hardy1-at-gmail.com Name: Prof. Numan Hardy

Organization: Curtin University, Australia

Title-Subject: [Filtered] EVO 18 SEM from ZEISS
Message: Dear All,

Does any one have an idea about the cheap EVO 18 SEM manufactured by Zeiss. I have limited budget of
US $ 80K and interested to buy either a second hand unit or EVO 18.
Please let me know if anybody has used this is Australia and from where I can buy it.

Please also comment on the quality of this model as I could not find any details on the web but have
seen this system in China.

Thanks,

Dr. Numan Hardy


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From: gabriella.chapman-at-materials.ox.ac.uk
Date: Fri, 27 Jan 2012 03:15:18 -0600
Subject: [Microscopy] Job opening: E M Facility Technical Manager, University of Oxford,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Department of Materials at the University of Oxford (UK) seeks a specialist facility technical manager of the highest calibre with the ability to provide strategic leadership for a large, world-class electron microscope facility.

Please follow the link: https://www.recruit.ox.ac.uk/pls/hrisliverecruit/erq_jobspec_version_4.jobspec?p_id=101946 †for more information

Closing date for applications is 24th February.






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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 27 Jan 2012 18:09:34 -0600
Subject: [Microscopy] viaWWW:Resins for immunocytochemistry

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Email: stempies-at-muohio.edu Name: Erin Stempinski

Organization: Miami University

Title-Subject: [Filtered] Resins for immunocytochemistry

Message: Dear Listserv,

I am looking at immunogold labeling proteins in Arabidopsis chloroplasts for TEM and was wondering
if anyone on the Listserv had experience with the differences in immunocytochemistry resins. I'm
interested in knowing how well Unicryl, LR White, and LR Gold have performed in other people's
experience with immunogold labeling.

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From: mike.bode-at-resaltatech.com
Date: Sun, 29 Jan 2012 12:27:45 -0600
Subject: [Microscopy] Re: EMs in the Movies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I don't know if this was mentioned earlier: My favorite EM in recent movies
is in "Invasion" with Nicole Kidman (2007). She plays a doctor trying to
figure out a virus, and they use an FEI TEM to look at the virus. The
interesting part is that when they move the digital camera in (side-mount),
the next scene shows ... color images of red blood cells swimming in a
liquid (blood) and being attacked by the virus. I guess you can call that
"poetic license". I am certainly not claiming that our cameras (which they
use) can do that :-)

Mike Bode, Ph.D.
ResAlta Research Technologies Corp.

2102 Beech Ct
Golden, CO 80401
USA

p: +1-303-234-9270
f: +1-303-202-6350
Mike.Bode-at-ResAltaTech.com
www.ResAltaTech.com





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From: common-at-msu.edu
Date: Sun, 29 Jan 2012 13:24:37 -0600
Subject: [Microscopy] Tucsen camera

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have experience with the Tucsen 9 MP microscope camera? Is
the computer interface easy to use? Is it easy to change image
resolution back and fourth for focusing and image capture? Is the
measuring software adequate? Can you set the white balance manually and
have it remain constant? How about overall image and video quality? If
you know of any issues with this camera, please let me know on or off list.

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From: oshel1pe-at-cmich.edu
Date: Mon, 30 Jan 2012 13:11:28 -0600
Subject: [Microscopy] Assembling a SEM

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***************************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
**any reply should go directly to the poster**
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Using the "reply" function in your email does *not* send your answer
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****************************************************************************************
} Date: Mon, 30 Jan 2012 10:43:44 -0800
} From: {AssociationManagement-at-microscopy.org}
} Reply-To: Jaimie Graham {jaimie_graham-at-cjuhsd.net}
} Subject: Ask-A-Microscopist
}
} Below is the result of your form, submitted on Monday, January 30,
} 2012 at 10:43:40 AM.
}
} realname - Jaimie Graham
} Email - jaimie_graham-at-cjuhsd.net
} ORGANIZATION - Ontario High School
} EDUCATION - 9-12th Grade High School
} LOCATION - Ontario High School
} SUBJECT_OF_QUESTION - SEM
} QUESTION - We have recently received a donated functioning Cambridge
} Instrument Stereoscan 360 SEM. I have used an SEM in college, so
} being one of the only teachers here on campus with any experience I
} have been given the task of figuring out how to put this microscope
} together. I was shipped to us in three main pieces and is currently
} wrapped in plastic. Most of it put together. Will I be able to
} assemble the microscope or do I need to find a company to put it
} together? Is it like a computer where it is simple to figure out
} what plugs into what or am I in way over my head??? Help!
}

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From: rok210-at-lehigh.edu
Date: Mon, 30 Jan 2012 14:33:19 -0600
Subject: [Microscopy] Assembling an SEM

Contents Retrieved from Microscopy Listserver Archives
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Re: donated Cambridge Instruments StereoScan 360 SEM: this looks like a good instrument and it should be nice to have in School, but it's not as easy as a computer to fit together. I would recommend getting professionals to put it together and switch it on. You are going to have to get a Safety certificate before you can use it in class, e.g. an SEM like this does produce X-rays.

The three parts are probably: (1) the electron optical column (heavy metal thing with suspension and a vacuum pumping system), (2) the control electronics console, with monitors and lots of cables around the back and (3) a crate with e.g. high voltage cable, mechanical pump(s), UPS battery system, X-ray detector, computer and accessories.

Try to find manuals, circuit diagrams and ideally installation/performance data; next look into a service company - try searching online. In the best case and with luck you might find someone who can fix things and get the SEM working for around $10K (about a weeks work).

Good luck
--
Robert Keyse
EM Facility
Lehigh University


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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 30 Jan 2012 18:57:07 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW:NESM February Meeting @ Saint-Gobain,

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Email: nesmicroscopy-at-gmail.com Name: NESM

Organization: The New England Society for Microscopy

Title-Subject: [Filtered] Reminder: NESM February Meeting -at-
Saint-Gobain, Northboro (Feb 15, 2012)

Message: Join NESM for our February Dinner Meeting hosted by
Saint-Gobainon February 15, 2012 -at- 5:30PM. The meeting is composed of a
Saint-Gobain facility tour, dinner, and two technical talks (see below
for talk information). Register now on our website nesmicroscopy.org .
You can also keep up with NESM activities by checking us out of
Facebook and Twitter.

Technical Talks:

"Crystals as Stacked Layers and the Infinite World of Intergrowths",
Charles Bateman, Ph.D., Saint-Gobain, Northboro, MA

"Microscopy tools: visual resolution of insect eyes", Paloma Gonzalez
Bellido, Ph.D., Marine Biological Laboratory, Woods Hole, MA


We hope you can make it!

Cheers,
NESM

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From: CGorman-at-hookecollege.com
Date: Tue, 31 Jan 2012 11:54:07 -0600
Subject: [Microscopy] Short Course Announcement: SEM

Contents Retrieved from Microscopy Listserver Archives
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Greetings Fellow Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering its scanning electron microscopy short course March 26-30, 2012.† In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.† For further SEM training details and registration information, please follow the link below:

http://www.hookecollege.com/courses/course.asp?COURSE_ID=42


Best regards-
__________________________________________________
Chris Gorman
Admissions Specialist
Hooke College of Applied Sciences
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7412(fax)
www.hookecollege.com




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From: lgordon-at-gmail.com
Date: Tue, 31 Jan 2012 14:33:38 -0600
Subject: [Microscopy] Staining Chitin for TEM

Contents Retrieved from Microscopy Listserver Archives
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Dear Listserv,

I have a chitin hydrogel that I would like to image in TEM. I did some
research and there are reports that standard EM heavy metal stains (osmium,
UA, lead citrate) will stain chitin, however, there are also papers that
indicate that chitin doesn't pick up these stains. I was wondering if
anyone has any experiance staining chitin for TEM.

Ideally, a protocol to stain sections on a grid would be best as I have
some un-stained sections (there is iron oxide in the gel which I wanted
to visualize without any stain). If this isn't possible then I can process
some additional samples.

Thanks very much,
Lyle

--
Lyle Gordon
Department of Materials Science and Engineering
Northwestern University

2220 Campus Drive
Evanston, IL 60208

Tel: (847) 491-3584
lgordon-at-u.northwestern.edu

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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 31 Jan 2012 16:01:07 -0600
Subject: [Microscopy] [Filtered] MicroscopyListserverviaWWW: Hitachi s-450 Parts

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Email: cmazareanu-at-yahoo.com Name: constantin

Title-Subject: [Filtered] Hitachi s-450

Message: Hi all!
I looking for spare/defective parts from a Hitachi s-450 SEM, free or at
low cost. I am interested mostly for microscope main board or other parts.
Thank you for reading my post.
Constantin


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From: benada-at-biomed.cas.cz
Date: Wed, 1 Feb 2012 02:27:30 -0600
Subject: [Microscopy] CM12 trouble

Contents Retrieved from Microscopy Listserver Archives
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Hello all,
I have following trouble. When starting our Philips CM12 after this night
short blackout I've noticed a strange compressed air noise somewhere around
the diffusion pump. I cannot find out which valve is responsible for it, because
OPCON console cannot be switched to the Vacuum page (pumps and valves scheme)
at this time due to a line scan calibration process.
Thanking you in advance for any suggestion.

Best regards Oldrich

==============================Original Headers==============================
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From: ben.micklem-at-pharm.ox.ac.uk
Date: Wed, 1 Feb 2012 04:05:52 -0600
Subject: [Microscopy] Re: CM12 trouble

Contents Retrieved from Microscopy Listserver Archives
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Dear Oldrich,

I would guess that the problem is coming from the o-rings on the fill and
drain caps of the diffusion pump. These hardened with time and the high
temperatures that they work under. They take on a triangular
cross-section. When the pump cools down, they shrink a fraction, then when
re-heated, because they are no longer elastic, they don't seal fully. The
o-rings will have quite a short life if you use Viton ones- you can buy
black perlast ones for 3x the price of Viton, or Kalrez ones for 4x the
price that have better resistance to oil and heat.



Ben


On 01/02/2012 08:34, "benada-at-biomed.cas.cz" {benada-at-biomed.cas.cz} wrote:

} --------------------------------------------------------------------------
}
} Hello all,
} I have following trouble. When starting our Philips CM12 after this night
} short blackout I've noticed a strange compressed air noise somewhere
} around
} the diffusion pump. I cannot find out which valve is responsible for it,
} because
} OPCON console cannot be switched to the Vacuum page (pumps and valves
} scheme)
} at this time due to a line scan calibration process.
} Thanking you in advance for any suggestion.
}
} Best regards Oldrich



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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 1 Feb 2012 08:05:11 -0600
Subject: [Microscopy] viaWWW:SEM. Extended continuous operation of Hitachi S4500

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Email: stevebuckingham-at-comcast.net Name: Steve Buckingham

Organization: Sakti3

Title-Subject: [Filtered] SEM. Extended continuous operation of Hitachi
S4500

Message: Hi All,
I want to run an extended continuous experiment that images a feature
for 24 hours or more. I'm using an Hitachi S4500 cold cathode FESEM and
the system trips (beam shut off due to emission drift?) or the it wants
a tip flash that stops my experiment. Can anyone tell me if it is
possible to run this kind of an experiment on this system and what I
need to do to achieve continuous operation of the system over 24 hours
or more.
Many thanks,
Steve

Steve Buckingham
Sakti3
Ann Arbor MI


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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 1 Feb 2012 08:06:29 -0600
Subject: [Microscopy] viaWWW:Benchmarking electron microscopy facility

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Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo

Organization: WVU

Title-Subject: [Filtered] Benchmarking
Message: Dear all:

At the present moment, we are reviewing the changes in our electron
microscopy facility that were implemented 6 months ago. Also, we are
ready to change unsuccessful methods for new ones. For that, I decided
to do some benchmarking to check other ideas, models and results in
other electron microscopy facilities that could help us improve our ways.

I have a group of topics/questions related to the subject and I would
like to discuss them with anyone that would be interested and available.
If you can help, please contact me directly through my email
(marcela.redigolo at mail.wvu.edu). I appreciate any help.

Thanks for your time!

Kindest regards,

Marcela.



---
Dr. Marcela Redigolo
Electron Microscopy Facility
WVU Shared Research Facilities
West Virginia University

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From: oshel1pe-at-cmich.edu
Date: Wed, 1 Feb 2012 09:32:24 -0600
Subject: [Microscopy] How to deaggregate liposomes for TEM?

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***************************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
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Using the "reply" function in your email does *not* send your answer
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****************************************************************************************

realname - Amanda Lever
Email - {mailto:lever.amanda1-at-gmail.com} lever.amanda1-at-gmail.com
ORGANIZATION - Draper Laboratory
EDUCATION - Graduate College
LOCATION - Boston, MA
SUBJECT_OF_QUESTION - Uranyl Acetate Stain
QUESTION - I am staining liposome with 2 % UA on plasma treated
Carbon Type-B grids. Is there anything I can add to my UA or grid to
reduce aggregation ? Thanks.



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From: one_twinklestar-at-yahoo.com.sg
Date: Wed, 1 Feb 2012 11:01:31 -0600
Subject: [Microscopy] Enquires on Purchasing Centrifuge and Rotator

Contents Retrieved from Microscopy Listserver Archives
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Dear All, our lab is trying to set up some facilities to prepare bio samples for TEM characterization.However, i have little knowledge on bio sample preparation for TEM. On the list of some equipment purchase, we are getting a centrifuge and a rotator. i would like to ask for advice

1) Is it critical to get a centrifuge with a swing out attachment for the centrifuge tube or a fix inclined angle type would be good enough?
2) I realise that the rotator have different degrees e.g 35, 45 and 55 degree, what is the use of different angle on these rotator?

Cheers,
Yee Yan
Nanyang Technological University
FACTS LAB


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4, 35 -- From: Tay Yee Yan {one_twinklestar-at-yahoo.com.sg}
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From: PWebster-at-hei.org
Date: Wed, 1 Feb 2012 11:29:55 -0600
Subject: [Microscopy] Enquires on Purchasing Centrifuge and Rotator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Yee Yan,

You bring up an interesting question that broadly affects us all, is it possible for a materials lab to successfully cater to biologists?

I see many universities in the US that set up a materials-dedicated EM lab and then invite biologists to use the equipment. While some of the machines on offer are top of the range microscopes, do they adequately serve the needs of the biologist?

Are there biologists on this server who use materials facilities and are successful on getting all their needs addressed? Do all materials labs have ultramicrotomes available, are there cryosectioning, high pressure freezing and freeze substitution machines available? Can 70 nm thin sections be successfully imaged in a TEM operating at 200kVa? Would the people running the facility be willing to turn down the expensive 200kVa machine to 80k?a for biologists to use?

I think the problem goes further than wondering if a centrifuge will be needed for a biologists - of course it will. Similarly with a rotator. A biologist would find this helpful when resin embedding the cell pellets they formed in the centrifuge. But where is the microwave processor for assisting with specimen embedding too?

Personally I think that biological EM will not survive if shared facilities do not provide expert support to biologists, with only limited knowledge of specimen preparation. There is more to what we do than what expensive microscopes provide.

Some facilities are very successful at catering for materials and biological sciences, but others just can't understand why biologists don't come flooding in. The biological scientists are there, but many of them do not know the best way to get results using the EM. These people need to be helped with advice and instruction, and they are also not usually on this listserver.

My advice would be to find a way to get someone on your staff who has expertise in biological EM and use them for the sort of advice you are looking for. At the very least, invite a biologist into your lab to offer advice on a consultative basis.

Best Regards,

Paul Webster
House Research Institute
2100 W 3rd St
Los Angeles CA 90057
USA


PS
Don't forget to register for our Immuno-Gold-Counting Course taking place in April.



-----Original Message-----
X-from: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Wed 2/1/2012 9:04 AM
To: Webster, Paul

Dear All, our lab is trying to set up some facilities to prepare bio samples for TEM characterization.However, i have little knowledge on bio sample preparation for TEM. On the list of some equipment purchase, we are getting a centrifuge and a rotator. i would like to ask for advice

1) Is it critical to get a centrifuge with a swing out attachment for the centrifuge tube or a fix inclined angle type would be good enough?
2) I realise that the rotator have different degrees e.g 35, 45 and 55 degree, what is the use of different angle on these rotator?

Cheers,
Yee Yan
Nanyang Technological University
FACTS LAB


==============================Original Headers==============================
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From: W.Muss-at-salk.at
Date: Wed, 1 Feb 2012 12:16:54 -0600
Subject: [Microscopy] Re: Enquires on Purchasing Centrifuge and Rotator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Yee Yan,

since I have googled your facility I now know that the primary target of
your Lab-Org will be Materials (http://facts.ntu.edu.sg/about.html ).
I would like to congratulate for the interesting facility map which also
is displayed at (http://facts.ntu.edu.sg/about_lablayout.html)

Seconding Paul Webster's recent reply (and underline the statements he
made) I personally feel that you
perhaps have to (then should) add a biological specimen preparation room
too... if you would like to attract Biologists to use your facility
(nevertheless a compliment for all those machines listed in the map...)

Regarding the secondary questions you were asking, I would like to answer
very briefly:
Centrifuge(s) (for biological preps preferably equipped with a cooling
device) with a "swing out rotor/rotator": would be necessary and IMHO
indispensable e.g. for blood centrifugations (esp. for "Buffy Coat"-Preps).
All spec.-prep. methods for pelleting cellular biological material
(e.g. blood, cell cultures, urinary sediments) will result in a homo-
genous pellet/sediment which can be asservated, conserved and finally
fixed with respective (chem..)fixative(s) and processed that way into
resin.
So called "angle-type" centrifuges might serve too, but produce no even,
orthogonally layered pellets (especially with regard to the even
distribution of "layers" if achievement of such are considered important).

Concerning the different types of "angles":
This means that the higher degree of angle the angle velocity and
thus the translational forces increase (at the end of the test tube)
due to the longer radius of the rotor. So it might well be you'll be
able to achieve higher g-forces on your specs with a 55į than with a 35į
rotor.
Hope this will be of any help with your "decision making"

best regards and greetings to Singapore,

Wolfgang MUSS PhD
SALZBURG, AUSTRIA


} -----UrsprŁngliche Nachricht-----
} Von: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg]
} Gesendet: Mittwoch, 01. Februar 2012 18:04
} An: MuŖ Wolfgang
} Betreff: [Microscopy] Enquires on Purchasing Centrifuge and Rotator
} -----------------------------------------------------------------------
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}
} Dear All, our lab is trying to set up some facilities to prepare bio
} samples for TEM characterization.
} However, i have little knowledge on bio sample preparation for TEM.
}
} On the list of some equipment purchase, we are getting a centrifuge and
} a rotator.
} i would like to ask for advice
}
} 1) Is it critical to get a centrifuge with a swing out attachment for
} the centrifuge tube or a fix inclined angle type would be good enough?
} 2) I realise that the rotator have different degrees e.g 35, 45 and 55
} degree, what is the use of different angle on these rotator?
}
} Cheers,
} Yee Yan
} Nanyang Technological University
} FACTS LAB
}
}
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From: jmiller-at-dunesciences.com
Date: Wed, 1 Feb 2012 13:22:25 -0600
Subject: [Microscopy] How to deaggregate liposomes for TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Amanda,
First I should disclose that I develop products at Dune Sciences, a
commercial supplier of functionalized TEM grids for advanced applications.
In response to your question restated below, functionalized Carbon grids
will improve and simplify the sample preparation of liposomes. The
positive charge on the grid disperses the liposomes during deposition and
plasma treating is not required as the grids are hydrophilic. You can learn
how functionalization chemistry works by going to the following link.
http://www.dunesciences.com/grids_CSMART.php


SUBJECT_OF_QUESTION - Uranyl Acetate Stain QUESTION - I am staining liposome
with 2 % UA on plasma treated Carbon Type-B grids. Is there anything I can
add to my UA or grid to reduce aggregation ? Thanks.


Thanks,

John Miller
Dune Sciences, Inc.
541-359-1959
jmiller-at-dunesciences.com


-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Wednesday, February 01, 2012 7:41 AM
To: jmiller-at-dunesciences.com

****************************************************************************
***********
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely not a member
the listserver, and **any reply should go directly to the poster** as well
as to the list.
Using the "reply" function in your email does *not* send your answer to the
person asking the question.
Please copy their email address from their question.
****************************************************************************
************

realname - Amanda Lever
Email - {mailto:lever.amanda1-at-gmail.com} lever.amanda1-at-gmail.com
ORGANIZATION - Draper Laboratory
EDUCATION - Graduate College
LOCATION - Boston, MA
SUBJECT_OF_QUESTION - Uranyl Acetate Stain QUESTION - I am staining liposome
with 2 % UA on plasma treated Carbon Type-B grids. Is there anything I can
add to my UA or grid to reduce aggregation ? Thanks.



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From: FMonson-at-wcupa.edu
Date: Wed, 1 Feb 2012 14:06:41 -0600
Subject: [Microscopy] Enquires on Purchasing Centrifuge and Rotator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have a 120kV FEI Tecnai 12 Twin TEM which was chosen to serve multiple purpose projects. On installation the scope was rated at 2nm. We have stereo-imaged 500nm sections, 70nm sections of epoxy-embedded silica gel impregnated with Cobalt oxide deposits, and nano-particulates on C-coated grids. For a relatively non-analytic, vanilla, 120kV instrument, I believe that is not bad. I routinely view thin sections (60-80nm) of biological tissue post-fixed with osmium and permit the 1k Gatan to add the contrast which is usually quite satisfactory. The vacuum is usually in the middle of 10-8 T which is also more than satisfactory. We did not spec a 'bio' lens configuration, because we wanted to be flexible.
We have a lab equipped as can be seen on our web site.

Most of our users are not biologists, but we can certainly do biologic specimens without any problem. The only difference I have imposed on the use of the TEM is that all biological specimens and those nano particles produced by wet chemistry and not presented in light alcohols are used with the LN2 coldfinger to prevent general contamination in the stage area.

Short of the accessories for vitreous quenching, the biggest difference will lie with the biological core facility that cannot be used by materials scientists - no matter what the size/expense.

Finally, having said all of the above, there are choices in instruments, because there is variety of need. One can stretch one's flexibility so far that it excludes the need one was supposed to address in the first place. A core facility with a Titan and a 120kV Bio-Twin would serve both material and biological investigators. Under what conditions would a biologist need wave-length resolution and not, in truth, be working on bio-materials.

As an example, if I had the money and I wanted to purchase a Titan with 10 years of service, I might spend it and have the package delivered to someone who has a discernible need. I, even when I try with diligence, cannot uncover a need/use for myself. So, I would not recommend such an acquisition, even if it were offered, if I could not worry up a need to have it. Then, of course, the ceiling here is not nearly high enough, and even though on bedrock, and lacking subways, there are still trucks and busses, AND, where I now need only two rooms for the TEM, I would need at least three for a Titan, and perhaps for a 200kV with no observation chamber as well.

The Dean of a college has a materials-oriented core facility for which s/he is responsible (services, maintenance, and cost recovery). S/he charges the Deans of other colleges whose Faculty use the facility. The President owns the Colleges and the cores, and spends money on an accounting scheme which issues bills for use to Departments and Individuals who have external funding. There is no mechanism to charge for undergraduate courses, which, thus, do not usually fit into such a scheme. So the core is limited to research use, and many bright students choose business and finance.

Cheers,

Fred Monson

P.S. The day is almost over, and I look forward to the continuing mechanical and optical attention I am paying to one of my antique Leitz 'Research' Microscopes. I could NEVER do that with a Titan, nor even with our Tecnai. How sad.... I, not a Tinker, Tailor, nor a Soldier be, but merely a mechanic - just like me Dad. God bless 'im!

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
Schmucker Science Center South
West Chester University, West Chester, PA 19383
610-738-0437

Home Page: http://cmirt.wcupa.edu
Scheduler: http://cmirt.wcupa.edu/cgi-bin/ureserve_gold.pl

If one looks long enough, one may actually see something frozen in time that flashes one's mind to a new state.
1. The day that I asked myself how the hydrogen atom's mass could actually be: 1.0008.
2. The day, while looking at a slide of some tissue, I saw a leukocyte that had been caught sneaking in or out of a capillary: http://www.annualreviews.org/doi/abs/10.1146/annurev-pathol-011110-130224

-----Original Message-----
X-from: PWebster-at-hei.org [mailto:PWebster-at-hei.org]
Sent: Wednesday, February 01, 2012 12:36 PM
To: Monson, Frederick

Dear Yee Yan,

You bring up an interesting question that broadly affects us all, is it possible for a materials lab to successfully cater to biologists?

I see many universities in the US that set up a materials-dedicated EM lab and then invite biologists to use the equipment. While some of the machines on offer are top of the range microscopes, do they adequately serve the needs of the biologist?

Are there biologists on this server who use materials facilities and are successful on getting all their needs addressed? Do all materials labs have ultramicrotomes available, are there cryosectioning, high pressure freezing and freeze substitution machines available? Can 70 nm thin sections be successfully imaged in a TEM operating at 200kVa? Would the people running the facility be willing to turn down the expensive 200kVa machine to 80k?a for biologists to use?

I think the problem goes further than wondering if a centrifuge will be needed for a biologists - of course it will. Similarly with a rotator. A biologist would find this helpful when resin embedding the cell pellets they formed in the centrifuge. But where is the microwave processor for assisting with specimen embedding too?

Personally I think that biological EM will not survive if shared facilities do not provide expert support to biologists, with only limited knowledge of specimen preparation. There is more to what we do than what expensive microscopes provide.

Some facilities are very successful at catering for materials and biological sciences, but others just can't understand why biologists don't come flooding in. The biological scientists are there, but many of them do not know the best way to get results using the EM. These people need to be helped with advice and instruction, and they are also not usually on this listserver.

My advice would be to find a way to get someone on your staff who has expertise in biological EM and use them for the sort of advice you are looking for. At the very least, invite a biologist into your lab to offer advice on a consultative basis.

Best Regards,

Paul Webster
House Research Institute
2100 W 3rd St
Los Angeles CA 90057
USA


PS
Don't forget to register for our Immuno-Gold-Counting Course taking place in April.



-----Original Message-----
X-from: one_twinklestar-at-yahoo.com.sg [mailto:one_twinklestar-at-yahoo.com.sg]
Sent: Wed 2/1/2012 9:04 AM
To: Webster, Paul

Dear All, our lab is trying to set up some facilities to prepare bio samples for TEM characterization.However, i have little knowledge on bio sample preparation for TEM. On the list of some equipment purchase, we are getting a centrifuge and a rotator. i would like to ask for advice

1) Is it critical to get a centrifuge with a swing out attachment for the centrifuge tube or a fix inclined angle type would be good enough?
2) I realise that the rotator have different degrees e.g 35, 45 and 55 degree, what is the use of different angle on these rotator?

Cheers,
Yee Yan
Nanyang Technological University
FACTS LAB


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From: AJBowling-at-dow.com
Date: Wed, 1 Feb 2012 14:29:21 -0600
Subject: [Microscopy] viaWWW:Resins for immunocytochemistry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Erin,

I use LR white almost exclusively for immunolabeling in plant tissue and have very good luck with it. The protocol that I use I got from Kevin Vaughn, so I would suggest that you check out a few of his papers. Some of his earlier work was on immunolabeling various proteins in chloroplasts, so they may be especially relevant to you, though the later papers may have slightly more up-to-date protocols.

If you have any trouble finding his papers or need more detail on any of the steps of the protocol, just let me know.

Andy




_____________________
Andrew J Bowling, PhD
Discovery Research
Dow AgroSciences
9330 Zionsville Rd
Indianapolis, IN 46268
317-337-3878







-----Original Message-----
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Email: stempies-at-muohio.edu Name: Erin Stempinski

Organization: Miami University

Title-Subject: [Filtered] Resins for immunocytochemistry

Message: Dear Listserv,

I am looking at immunogold labeling proteins in Arabidopsis chloroplasts for TEM and was wondering
if anyone on the Listserv had experience with the differences in immunocytochemistry resins. I'm
interested in knowing how well Unicryl, LR White, and LR Gold have performed in other people's
experience with immunogold labeling.

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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 1 Feb 2012 14:44:39 -0600
Subject: [Microscopy] viaWWW:Thanks!!! [Benchmarking electron microscopy facility]

Contents Retrieved from Microscopy Listserver Archives
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Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo

Organization: WVU

Title-Subject: [Filtered] Thanks!!! [Benchmarking electron microscopy
facility]

Message: Dear all:

I just want to thank you for the replies I got. I'm contacting each one
individually. Thanks again for the replies so far.
Marcela.

----


Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo
Organization: WVU
Title-Subject: [Filtered] Benchmarking
Message:
Dear all:
At the present moment, we are reviewing the changes in our electron
microscopy facility that were implemented 6 months ago. Also, we are
ready to change unsuccessful methods for new ones. For that, I decided
to do some benchmarking to check other ideas, models and results in
other electron microscopy facilities that could help us improve our ways.
I have a group of topics/questions related to the subject and I would
like to discuss them with anyone that would be interested and available.
If you can help, please contact me directly through my email
(marcela.redigolo at mail.wvu.edu). I appreciate any help.
Thanks for your time!
Kindest regards,
Marcela.
---
Dr. Marcela Redigolo
Electron Microscopy Facility
WVU Shared Research Facilities
West Virginia University


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From: beth-at-plantbio.uga.edu
Date: Wed, 1 Feb 2012 15:35:04 -0600
Subject: [Microscopy] staining chitin

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Hi Lyle and all,
Try the protocol in Lingle, W.L. (1989). Enhanced Staining of the
Basidiomycete Panellus stypticus Prepared for TEM by Freeze
Substitution. Cryotogamic Botany 1, 236-242. Wilma did a modification
of the Thiery stain and this method stains chitin nicely. I couldn't
find the journal article on-line but I can snail mail a copy to you if
you'd like it.
best,
Beth


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From: rosemary.white-at-csiro.au
Date: Wed, 1 Feb 2012 16:11:28 -0600
Subject: [Microscopy] Re: staining chitin

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Just a thought - the fluorescent lectins are very good at binding
specifically to chitin, e.g. fluorescent wheat germ agglutinin. Would a
gold-tagged one work in TEM? I don't know if they exist, but it would be
very straightforward to do the staining.
cheers,
Rosemary


Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
M 61 2 420 972 028
E rosemary.white-at-csiro.au



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From: pwebster-at-hei.org
Date: Wed, 1 Feb 2012 16:25:10 -0600
Subject: [Microscopy] staining chitin

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Hi Rosemary,

Good idea with the lectins. However, I would not use lectins directly
coupled to gold particles. Instead, for EM labeling I would probably apply
unbound lectin to the sections and then specific anti-lectin antibodies
followed by the gold probe. Then there would be no concern about the gold
particles disassociating from the lectin during storage.

Of course, if going the antibody route, why not use anti-chitin antibodies?

Regards

Paul.



Paul Webster, Ph.D.
House Research Institute
2100 West Third Street
Los Angeles
CA 90057
(213) 273 8026



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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 2 Feb 2012 03:32:45 -0600
Subject: [Microscopy] viaWWW:Update - Benchmarking EM Facility

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Email: marcela.redigolo-at-mail.wvu.edu Name: Marcela Redigolo

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Title-Subject: [Filtered] Update - Benchmarking EM Facility

Message: Dear all:

I am receiving valuable information and want to thank you all for your
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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 2 Feb 2012 13:41:50 -0600
Subject: [Microscopy] viaWWW:TEM Lithium Detection By EELs Analysis

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Email: korinek-at-mcmaster.ca Name: Andreas Korinek

Organization: McMaster University

Title-Subject: [Filtered] TEM Lithium Detection By EELs Analysis

Message: I want to detect Lithium in a FIB section at 300 kV. The FIB
section is approximately 0.8 MFP thick.
I'm using a FEI Titan microscope at 300 kV, equipped with a Tridium GIF.
I know that there is a Li signal at 55 eV, I can detect some signal
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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 2 Feb 2012 13:42:45 -0600
Subject: [Microscopy] viaWWW:EELS quantification error and spectra normalization

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Email: leo.erich-at-yahoo.com Name: Leo

Title-Subject: [Filtered] EELS quantification error and spectra
normalization
Message: Dear All

I would like to know :

1) how I can reasonably determine the error bar when I perform
spectrum-image (EELS line scan ) measurement across the interface or
grain boundary ?
2)how to perform EELS spectra normalization in Digital Micrograph ?
I would appreciate very much to have your opinion.


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From: microscopylistserver-noreply-at-microscopy.com
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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 2 Feb 2012 13:43:41 -0600
Subject: [Microscopy] viaWWW:Postdoctoral Position - University of Tennessee Health Science

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Email: aphill48-at-uthsc.edu Name: Aquila Phillips

Organization: University of Tennessee Health Science Center

Title-Subject: [Filtered] Postdoctoral Position - University of
Tennessee Health Science Center

Message: Postdoctoral positions: Several postdoctoral positions are
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From: frank_karl-at-ardl.com
Date: Fri, 3 Feb 2012 08:23:07 -0600
Subject: [Microscopy] more wired microscopy

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It's Friday and if the prospect of a week-end isn't enough, Wired has come through. No, they're not all photographs. The article is about Science Visualizations and some great images. I'm disappointed there's no fusion preps in PLM but the cucumber skin barbs make up for it.
Here's the link.

http://www.wired.com/wiredscience/2012/02/science-visualizations-2011/?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+wired%2Findex+%28Wired%3A+Index+3+%28Top+Stories+2%29%29&pid=3031

One can't help wonder what the folks at Wired think of the sudden influx of web traffic for specific article.

Frank Karl
Microscopist
ARDL
2887 Gilchrist Road
Akron, Ohio 44305
330-794-6600



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From: bbandli-at-d.umn.edu
Date: Fri, 3 Feb 2012 08:30:37 -0600
Subject: [Microscopy] Re: viaWWW:SDD - standby or operate mode

Contents Retrieved from Microscopy Listserver Archives
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Hi Patricia,

I posed the same question to my Oxford service engineer and he told me
that they recommend leaving the detector in operate mode. I haven't
been able to detect any adverse effects in detector performance.

Bryan

On Thu, Feb 2, 2012 at 1:53 PM,
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} Title-Subject: [Filtered] SDD - standby or operate mode
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} Message: We have a new SDD Oxford detector on our S-4700. I was
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--
_________________________________________________________________
Bryan R. Bandli

Research Instrumentation Laboratory Manager
University of Minnesota, Duluth
229 Heller Hall
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218-726-7362
==================================================================


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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 3 Feb 2012 09:35:55 -0600
Subject: [Microscopy] viaWWW:counting virus particles

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Email: mary.walker-at-csl.com.au Name: mary walker

Organization: csl limited

Title-Subject: [Filtered] counting virus particles

Message: Do any listers have experience with counting virus particles
with latex beads?
Is it possible to do better than order of magnitude?
What is the minimum number of particles per ml that can be counted?
What is the lower limit?

thanks in advance

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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 3 Feb 2012 09:36:29 -0600
Subject: [Microscopy] viaWWW:TEM specimen preparation for semiconductors

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Email: dvsridhararao-at-yahoo.co.in Name: Dr.D.V.Sridhara Rao

Organization: DMRL, Hyderabad

Title-Subject: [Filtered] TEM specimen preparation for semiconductors

Message: Sir/Madam,

We are in the process of procuring a polishing equipment for preparing
plan-view and cross-sectional TEM specimens of semiconductor thin films,
especially III-arsenides and III-Nitrides.
In this context, we have come across the Buehler's product MPC 2000
(weblink given below):

http://www.buehler.com/productinfo/electronics/pdfs/MPC_2000.pdf

May I request the users of this equipment/those familiar with this
equipment, to kindly give their valuable feed back on the
functioning/suitability of this equipment for TEM specimen preparation?
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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 3 Feb 2012 09:36:57 -0600
Subject: [Microscopy] viaWWW:immunohistochemistry with anti-VEGF antibody

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Email: dieter.bosshardt-at-zmk.unibe.ch Name: Dieter Bosshardt

Organization: School of Dental Medicine, University of Bern, Switzerland

Title-Subject: [Filtered] immunohistochemistry with anti-VEGF antibody

Message: Hi
I was wondering if anyone has any experience of immunohistochemical
staining for VEGF in the human dental pulp? I am currently trying to
stain the vasculature, but am experiencing a lot of background collagen
staining too, making differentiation impossible!
I am using Polyclonal Goat IgG VEGF antibody, species reactivity human
(R&D systems), together with their HRP-AEC kit. On the positive and
negative control tissues (placenta) it has worked really well, with the
blood vessels outlined clearly by the stain:

My tissue of interest is, however the human pulp, I am using 5μm
sections of teeth which have been fixed in formalin for 48hrs,
decalcified in 10% formic acid and then processed and paraffin embedded.
Despite the VEGF protocol working really well on the control tissues the
background staining (mainly collagen) is really high in my tissue
sections at both the recommended working dilution (1:20, and a much
lower dilution 1:100):

Does anyone have any experience of staining for VEGF in such tissue
sections, and/or do they have any practical advice about how to
eliminate/reduce dramatically this background staining?
Kind regards
Dieter Bosshardt


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From: bozzola-at-siu.edu
Date: Fri, 3 Feb 2012 11:49:19 -0600
Subject: [Microscopy] viaWWW:SDD - standby or operate mode

Contents Retrieved from Microscopy Listserver Archives
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Hi Patricia,

We also have an Oxford SDD and the technologist who installed it
recommended that we keep it in standby mode when not being used in the
near future.

Translation: if it would be used within 12-24 hr, keep it chilled. Any
longer periods, go to standby.

My personal opinion is keeping it chilled all the time is putting an
unnecessary "strain" on the Peltier electronics.

--
John J. Bozzola, Ph.D.
Professor & Blissfully Retired Director of IMAGE
Integrated Microscopy & Graphics Expertise
Southern Illinois University
Carbondale, IL  62901


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From: nicholls-at-uic.edu
Date: Fri, 3 Feb 2012 11:53:09 -0600
Subject: [Microscopy] viaWWW:SDD - standby or operate mode

Contents Retrieved from Microscopy Listserver Archives
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Patricia

I have spoken with Oxford's applications people and they suggest leaving it
in Standby except when needed. It takes no more than 5 minutes to cool down
to the operating temperature.

Alan

At 08:31 AM 2/3/2012, bbandli-at-d.umn.edu wrote:



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From: marie.cantino-at-uconn.edu
Date: Fri, 3 Feb 2012 13:07:15 -0600
Subject: [Microscopy] TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
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In recent years we have been getting requests to image functionalized
nanoparticles, such as 10 nm gold with proteins or peptides attached.
Generally the clients want to know how thick or large the organic
"shell" is, or how the protein/peptide is arranged on the surface.
Most of our experience has been with either inorganic particles (which
we view without staining) or larger organic structures such as viral
procapsids or large proteins (e.g., myosin) or protein assemblies,
which we negative stain. My questions for this list are as follows:

- have others on the list had good luck imaging these kinds of
conjugates at the EM level, and if so, using what techniques?
- how much organic material must one have associated with the
nanoparticle to see it by negative staining or other methods? For
example, how hard is it to detect a 150 kD protein on a 10 nm gold
particle? An example would be IgG attached to a 10 nm gold particle.
- can you suggest any publications or review articles on the subject?
For some reason I'm not having much luck with my Pubmed search
strategies. A recent paper by Cao and Mao seems to deal mainly with
small gold particles on much larger protein aggregates, but perhaps
there are others out there.

Many thanks (in advance) for your suggestions.

Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369


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From: rbeavers-at-mail.smu.edu
Date: Fri, 3 Feb 2012 13:31:33 -0600
Subject: [Microscopy] Process of building "Academic Core Facilities"

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Group,

Would like to explore the experiences and words of wisdom on the idea of having a "core analytical facility " on a University campus.
Questions I am exploring are:
How is this idea presented to administrators as a needed addition to support research activities across many disciplines? Or is it?
How are such facilities managed and what are reasonable staffing levels.
What drives the tool set? Is it internal existing research or potential outside research partnerships?
Finally most important is addressing how to pay for and continually support such a facility?

Thanks for any thoughts or ideas on the subject.

Roy Beavers
Southern Methodist University
Department of Earth Sciences
3225 Daniel Ave
Dallas, TX† 75205
Voice: 214-768-2756
Email: rbeavers-at-smu.edu



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From: edelmare-at-muohio.edu
Date: Fri, 3 Feb 2012 13:51:34 -0600
Subject: [Microscopy] Re: Staining Chitin for TEM

Contents Retrieved from Microscopy Listserver Archives
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Lyle:

I have done a lot of work with Fungal samples whose walls are mostly
chitin. The walls routinely stained well with OsO4, Uac, and PbCit - sorry I
never did a test to see which predominantly stained the chitin. However, I
have used Barium Permanganate specifically for stainging fungal walls for
TEM and it does a great job. Original reference is:

Hoch, H. C. 1977. Use of permanganate of increase the electron opacity
of fungal walls. Mycologia 69:1209-2113.


On 31 Jan 2012 at 15:34, lgordon-at-gmail.com wrote:

}
}
}
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} Dear Listserv,
}
} I have a chitin hydrogel that I would like to image in TEM. I did some
} research and there are reports that standard EM heavy metal stains
} (osmium, UA, lead citrate) will stain chitin, however, there are also
} papers that indicate that chitin doesn't pick up these stains. I was
} wondering if anyone has any experiance staining chitin for TEM.
}
} Ideally, a protocol to stain sections on a grid would be best as I
} have some un-stained sections (there is iron oxide in the gel which I
} wanted to visualize without any stain). If this isn't possible then I
} can process some additional samples.
}
} Thanks very much,
} Lyle
}
} --
} Lyle Gordon
} Department of Materials Science and Engineering
} Northwestern University
}
} 2220 Campus Drive
} Evanston, IL 60208
}
} Tel: (847) 491-3584
} lgordon-at-u.northwestern.edu
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Richard E. Edelmann, Ph.D., Director
Center for Advanced Microscopy & Imaging
9C Upham Hall
Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-muohio.edu
http://www.cami.muohio.edu


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From: jfmjfm-at-umich.edu
Date: Fri, 3 Feb 2012 15:42:22 -0600
Subject: [Microscopy] 3rd Topical Conference on EBSD

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

On behalf of the Microanalysis Society (MAS), I invite you to the 3rd Topical Conference on EBSD, which will be held at Carnegie Mellon University on June 19-21, 2012. We have fantastic new speakers, and we are once again providing a comprehensive tutorial on EBSD that will include live demonstrations. For more information, and to register, please visit our website:

http://www.microbeamanalysis.org/topical-conferences/ebsd-2012/

Space is limited, so please register early. We look forward to seeing you in Pittsburgh!

Best regards,
Andrew Deal

MAS Director,
EBSD 2012 Organizing Committee Chair

Thanks,
Andy
********************************************
Andrew Deal, PhD
Materials Scientist
GE Global Research
One Research Circle
Building K1, Room 1D-37A
Niskayuna, NY 12309
US
T +1 518 387 5456
F +1 518 387 6905
********************************************



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From: microscopylistserver-noreply-at-microscopy.com
Date: Fri, 3 Feb 2012 16:48:48 -0600
Subject: [Microscopy] viaWWW:MAS EBSD 2012 Topical Conference

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This Question/Comment was submitted to the Microscopy Listserver
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Email: deal-at-research.ge.com Name: Andrew Deal

Organization: Microanalysis Society

Title-Subject: [Filtered] EBSD 2012 Topical Conference

Message: Dear Colleagues,

On behalf of the Microanalysis Society (MAS), I invite you to the 3rd
Topical Conference on EBSD, which will be held at Carnegie Mellon
University on June 19-21, 2012. We have fantastic new speakers, and we
are once again providing a comprehensive tutorial on EBSD that will
include live demonstrations. For more information, and to register,
please visit our website:
http://www.microbeamanalysis.org/topical-conferences/ebsd-2012/

Space is limited, so please register early. We look forward to seeing
you in Pittsburgh!

Best regards,
Andrew Deal

MAS Director,
EBSD 2012 Organizing Committee Chair


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From: dsherman-at-purdue.edu
Date: Sat, 4 Feb 2012 09:49:39 -0600
Subject: [Microscopy] Materials Science Department Head position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi All,

For any of you out there who think they might like to try administration, a
position has opened up at Purdue and we (other interested microscopists on
campus) are hoping for someone who is a strong advocate of microscopy.

Take a look and pass this on if you know of someone who might fit the bill.

++++++++++++++++++++++++++++++++++++++++++++++

Head and Professor
School of Materials Engineering

Purdue University is seeking nominations and applications for the position
of Head of the School of Materials Engineering. A dynamic leader is sought
to advance the SchoolĻs nationally-ranked program and to enhance its
international and national impact through a continuing commitment to
excellence in discovery, learning and engagement. The Head provides vision
and leadership to the faculty, students, staff, alumni, and other
stakeholders of the School, and will have the opportunity to shape and
implement the strategic plan for the School and the College of Engineering.

The Head must have a Ph.D. in Materials Science and Engineering or related
disciplines, qualify for an appointment at the full professor level with
tenure, have a distinguished academic, government or industrial record, and
demonstrate strong leadership and collaborative skills. Candidates should
have a clear understanding of the current needs and future direction of the
materials science and engineering profession, possess a commitment to
diversity and collaboration, and be skilled in administration, student
relations, mentoring, and alumni development.

The faculty members in the School of Materials Engineering are located in
the Neil Armstrong Hall of Engineering and the Birck Nanotechnology Center,
which provide outstanding facilities for teaching and research. The School
currently has 116 undergraduate students, 89 graduate students and postdocs,
and 20 faculty members. The SchoolĻs faculty members have strong connections
to research centers in PurdueĻs Discovery Park, as well as with the Colleges
of Agriculture, Liberal Arts, Pharmacy, Management, and Science.

The College of Engineering at Purdue consists of over 7300 undergraduate
students, nearly 2,900 graduate students, and 359 faculty members. Purdue is
one of the nationĻs leading land-grant universities with a full range of
academic majors and an enrollment of over 40,000 students on the West
Lafayette campus.

An application should include: (1) a three-page personal statement
addressing the applicant's vision, administrative philosophy, experience and
qualifications; (2) a curriculum vitae; and (3) names and contact
information for at least three references. Applications and inquiries will
be kept confidential. Applicants will be notified before references are
contacted. Electronic submission is preferred and should be uploaded at
https://engineering.purdue.edu/Engr/InfoFor/Employment. Screening will
commence February 1, 2012, and continue until this position is filled.
Nominations and questions regarding the position can be addressed to Chair,
Materials Engineering Head Search Committee, Purdue University, Neil
Armstrong Hall of Engineering, 701 W. Stadium Avenue, West Lafayette, IN
47907-2045, Phone: 765-494-5012, Email: mse-search-at-ecn.purdue.edu. A
background check will be required for employment in this position. Purdue
University is an equal opportunity/equal access/affirmative action employer
fully committed to achieving a diverse workforce.

Debby

--
Debby Sherman, Interim Director Phone: 765-494-6666
Life Science Microscopy Facility FAX: 765-494-5896
Purdue University E-mail: dsherman-at-purdue.edu
S-052 Whistler Building
170 S. University Street
West Lafayette, IN 47907
http://www.ag.purdue.edu/facilities/microscopy/



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From: seybert-at-biophysik.org
Date: Mon, 6 Feb 2012 02:47:20 -0600
Subject: [Microscopy] Looking for: Inner lid of Sorvall RC2-B

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,
We have an otherwise perfectly fine Sorvall RC2-B which failed it's recent safety check because the inner plastic cover of the lid has a crack. Of course, no parts can be found for that centrifuge anymore.
Thus, if you are trashing your old RC2-B, it would be great if we could get the plastic cover which is just screwed to the lid from the inside.

Thanks in advance for any help,

Anja Seybert
Research Scientist
Frankfurt am Main, Germany




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From: nizets2-at-yahoo.com
Date: Mon, 6 Feb 2012 03:43:21 -0600
Subject: [Microscopy] viaWWW:counting virus particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mary!

I am afraid I am not a specialist of this technique but I know how it works and I would like to just make some general comments.
Others with more experience may want to give a more precise answer.

This method involves statistical analysis, which means the more you sample, the more precise your number.
In other words, the precision of your analysis will basically depend on how much time you want to put into your counting (how many grids/fields you actually count).
Based on that, I assume that you may be able to count fewer numbers (decrease your lower limit) by counting many grids.

Best regards,
Stephane


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Email: mary.walker-at-csl.com.au Name: mary walker

Organization: csl limited

Title-Subject: [Filtered] counting virus particles

Message: Do any listers have experience with counting virus particles
with latex beads?
Is it possible to do better than order of magnitude?
What is the minimum number of particles per ml that can be counted?
What is the lower limit?

thanks in advance

† Login Host: 203.10.36.68
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From: nizets2-at-yahoo.com
Date: Mon, 6 Feb 2012 04:28:31 -0600
Subject: [Microscopy] TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Marie,

I like these projects which put the techniques to their limits.
I think the reason why you have difficulties finding publications on this matter and that the question is not trivial at all.
First of all, and to start with a relaxed note, the mixing of kDa und nm in one sentence made me smile because it makes as much sense as asking if 1kg of something fits in one meter.
The Dalton is a unit of mass and does not give a good indication of the dimension or the shape of a molecule.
That said, I seem to remember from the past answers of our emminent immunoglobulinologist Jan Leunissen that the size of an IgG is several nm, I think more than 5nm.
Which means, in your case, that you should not expect more that a few molecules per nanoparticle, except if they form multilayers.
As for the techniques, I wonder if a SEM could resolve this structure. Although I have experience with analytical low res SEMs, I don't know the limits of modern FEG SEMs.
I would be happy if some listers would care to share†their opinion on this matter.
It seems that AFM (atomic force microscopy) would be able to resolve this structure, but I wonder if it would be possible in this condition because AFM works on flat substrate and here you have round particles.
It would probably be possible to find some answers using cry-microscopy, the same way scientists use it to resolve viral structures at several anstrom resolution. It probably depends on how much time and money your clients are wanting to spend and how critical this information is for them.

Regards,
Stephane

----- Original Message -----
X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu}
To: nizets2-at-yahoo.com
Cc:
Sent: Friday, February 3, 2012 8:10 PM

In recent years we have been getting requests to image functionalized†
nanoparticles, such as 10 nm gold with proteins or peptides attached.†
Generally the clients want to know how thick or large the organic†
"shell" is, or how the protein/peptide is arranged on the surface.†
Most of our experience has been with either inorganic particles (which†
we view without staining) or larger organic structures such as viral†
procapsids or large proteins (e.g., myosin) or protein assemblies,†
which we negative stain.† My questions for this list are as follows:

- have others on the list had good luck imaging these kinds of†
conjugates at the EM level, and if so, using what techniques?
- how much organic material must one have associated with the†
nanoparticle to see it by negative staining or other methods?† For†
example, how hard is it to detect a 150 kD protein on a 10 nm gold†
particle?† An example would be IgG attached to a 10 nm gold particle.
- can you suggest any publications or review articles on the subject?†
For some reason I'm not having much luck with my Pubmed search†
strategies.† A recent paper by Cao and Mao seems to deal mainly with†
small gold particles on much larger protein aggregates, but perhaps†
there are others out there.

Many thanks (in advance) for your suggestions.

Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369


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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 6 Feb 2012 08:08:03 -0600
Subject: [Microscopy] viaWWW:TEM : Focusing Problem.

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Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar

Title-Subject: [Filtered] TEM : Focusing Problem.

Message: Image is not getting focused. The image is drastically moving
while focusing, it moving highly even at low focus step & at low
magnification.

So how to resolve this problem.

Login Host: 14.139.97.76
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From: colijn.1-at-osu.edu
Date: Mon, 6 Feb 2012 08:37:46 -0600
Subject: [Microscopy] Re: viaWWW:TEM : Focusing Problem.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Ravi,

Movement of the image when changing the objective lens (i.e. FOCUS) is
an indication that the beam is going through the objective lens at an
angle, i.e. not on the optic axis. The correction step is usually
referred to as a "Rotation Calibration" or Objective lens "Current
Centering".

It is difficult to describe the exact steps to correct this without
knowing which microscope you have since the knobs will vary from
microscope to microscope.

Many microscopes have a direct alignment step labelled "rotation center"
which oscillates the objective lens. Start with the image close to
focus, engage the "rotation center" function (start the objective lens
current oscillation) and adjust the correction knobs to minimize the
image movement.

For a non-computerized microscope, there may be a set of knobs labelled
"rotation center". In this case, focus the image and set a recognizable
feature in the center. Then defocus and watch the feature shift away
from the center. Use the "rotation center" knobs to recenter the
feature while the image is defocused. Repeat if necessary.

Good luck,
Henk

At 2/6/2012 9:11 AM, microscopylistserver-noreply-at-microscopy.com wrote:
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} Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar
}
} Title-Subject: [Filtered] TEM : Focusing Problem.
}
} Message: Image is not getting focused. The image is drastically moving
} while focusing, it moving highly even at low focus step& at low
} magnification.
}
} So how to resolve this problem.
}
} Login Host: 14.139.97.76
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--
Hendrik O. Colijn
www.ceof.ohio-state.edu
OSU Campus Electron Optics Facility colijn.1-at-osu.edu
040 Fontana Labs (614) 292-0674 (V)
116 W. 19th Ave. (614) 292-7523 (F)
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"Time is that quality of nature which keeps things from happening all at
once. Lately it doesn't seem to be working."

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From: marie.cantino-at-uconn.edu
Date: Mon, 6 Feb 2012 08:40:11 -0600
Subject: [Microscopy] TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stefan,

I do understand the difference between mass and length.

The problem is that the clients usually can only give us the molecular
weight and (if we're lucky) the number of molecules per particle. If
they knew how the proteins/peptides were arranged or the linear
dimensions of the coating they wouldn't need to come to me. What I'm
trying to find out from the listserver or the literature is whether we
can reasonably expect to be able to detect a medium sized protein such
as IgG on an electron dense gold particle, given the various artifacts
of techniques such as negative staining.

We only have TEM (w/a tungsten emitter) and FESEM in our facility, but
I can send them elsewhere for AFM.

Marie

On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote:

}
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} America
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} Hi Marie,
}
} I like these projects which put the techniques to their limits.
} I think the reason why you have difficulties finding publications on
} this matter and that the question is not trivial at all.
} First of all, and to start with a relaxed note, the mixing of kDa
} und nm in one sentence made me smile because it makes as much sense
} as asking if 1kg of something fits in one meter.
} The Dalton is a unit of mass and does not give a good indication of
} the dimension or the shape of a molecule.
} That said, I seem to remember from the past answers of our emminent
} immunoglobulinologist Jan Leunissen that the size of an IgG is
} several nm, I think more than 5nm.
} Which means, in your case, that you should not expect more that a
} few molecules per nanoparticle, except if they form multilayers.
} As for the techniques, I wonder if a SEM could resolve this
} structure. Although I have experience with analytical low res SEMs,
} I don't know the limits of modern FEG SEMs.
} I would be happy if some listers would care to share their opinion
} on this matter.
} It seems that AFM (atomic force microscopy) would be able to resolve
} this structure, but I wonder if it would be possible in this
} condition because AFM works on flat substrate and here you have
} round particles.
} It would probably be possible to find some answers using cry-
} microscopy, the same way scientists use it to resolve viral
} structures at several anstrom resolution. It probably depends on how
} much time and money your clients are wanting to spend and how
} critical this information is for them.
}
} Regards,
} Stephane
}
} ----- Original Message -----
} X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu}
} To: nizets2-at-yahoo.com
} Cc:
} Sent: Friday, February 3, 2012 8:10 PM
} Subject: [Microscopy] TEM of functionalized nanoparticles
}
}
}
}
} ----------------------------------------------------------------------------
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} America
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}
} In recent years we have been getting requests to image functionalized
} nanoparticles, such as 10 nm gold with proteins or peptides attached.
} Generally the clients want to know how thick or large the organic
} "shell" is, or how the protein/peptide is arranged on the surface.
} Most of our experience has been with either inorganic particles (which
} we view without staining) or larger organic structures such as viral
} procapsids or large proteins (e.g., myosin) or protein assemblies,
} which we negative stain. My questions for this list are as follows:
}
} - have others on the list had good luck imaging these kinds of
} conjugates at the EM level, and if so, using what techniques?
} - how much organic material must one have associated with the
} nanoparticle to see it by negative staining or other methods? For
} example, how hard is it to detect a 150 kD protein on a 10 nm gold
} particle? An example would be IgG attached to a 10 nm gold particle.
} - can you suggest any publications or review articles on the subject?
} For some reason I'm not having much luck with my Pubmed search
} strategies. A recent paper by Cao and Mao seems to deal mainly with
} small gold particles on much larger protein aggregates, but perhaps
} there are others out there.
}
} Many thanks (in advance) for your suggestions.
}
} Dr. Marie E. Cantino
} Associate Professor of Physiology and Neurobiology
} Director, Electron Microscopy Laboratory
} University of Connecticut, Unit 3242
} Phone: 860-486-3588
} Fax: 860-486-6369
}
}
} ==============================Original
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} }
} 15, 42 -- Date: Mon, 6 Feb 2012 02:28:29 -0800 (PST)
} 15, 42 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 15, 42 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
} 15, 42 -- Subject: Re: [Microscopy] TEM of functionalized
} nanoparticles
} 15, 42 -- To: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu}
} 15, 42 -- Cc: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
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Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369


==============================Original Headers==============================
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From: cdh1-at-cdc.gov
Date: Mon, 6 Feb 2012 09:21:15 -0600
Subject: [Microscopy] TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Marie,

I am not certain this answers your question but you should be able to detect IgG attached to an electron dense gold particle by negative stain EM. Antibodies are commonly seen attached to virus particles and to colloidal gold particles when negative stain EM is done. Anything you would likely see in your indicated study by AFM can be seen by negative stain EM. All techniques have various artifacts including light-scatter, thin-section EM, AFM, FESEM, and cryo-EM. The key to EM and techniques in general is to learn what is real and what is artifact. It often takes time and work to develop proficiency with many techniques though. Good luck with this , it should be possible.

Charles Humphrey, Ph. D.
CDC
Atlanta, GA 30333

-----Original Message-----
X-from: marie.cantino-at-uconn.edu [mailto:marie.cantino-at-uconn.edu]
Sent: Monday, February 06, 2012 9:49 AM
To: Humphrey, Charles (CDC/OID/NCEZID)

Hi Stefan,

I do understand the difference between mass and length.

The problem is that the clients usually can only give us the molecular weight and (if we're lucky) the number of molecules per particle. If they knew how the proteins/peptides were arranged or the linear dimensions of the coating they wouldn't need to come to me. What I'm trying to find out from the listserver or the literature is whether we can reasonably expect to be able to detect a medium sized protein such as IgG on an electron dense gold particle, given the various artifacts of techniques such as negative staining.

We only have TEM (w/a tungsten emitter) and FESEM in our facility, but I can send them elsewhere for AFM.

Marie

On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote:

}
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} Hi Marie,
}
} I like these projects which put the techniques to their limits.
} I think the reason why you have difficulties finding publications on
} this matter and that the question is not trivial at all.
} First of all, and to start with a relaxed note, the mixing of kDa und
} nm in one sentence made me smile because it makes as much sense as
} asking if 1kg of something fits in one meter.
} The Dalton is a unit of mass and does not give a good indication of
} the dimension or the shape of a molecule.
} That said, I seem to remember from the past answers of our emminent
} immunoglobulinologist Jan Leunissen that the size of an IgG is several
} nm, I think more than 5nm.
} Which means, in your case, that you should not expect more that a few
} molecules per nanoparticle, except if they form multilayers.
} As for the techniques, I wonder if a SEM could resolve this structure.
} Although I have experience with analytical low res SEMs, I don't know
} the limits of modern FEG SEMs.
} I would be happy if some listers would care to share their opinion on
} this matter.
} It seems that AFM (atomic force microscopy) would be able to resolve
} this structure, but I wonder if it would be possible in this condition
} because AFM works on flat substrate and here you have round particles.
} It would probably be possible to find some answers using cry-
} microscopy, the same way scientists use it to resolve viral structures
} at several anstrom resolution. It probably depends on how much time
} and money your clients are wanting to spend and how critical this
} information is for them.
}
} Regards,
} Stephane
}
} ----- Original Message -----
} X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu}
} To: nizets2-at-yahoo.com
} Cc:
} Sent: Friday, February 3, 2012 8:10 PM
} Subject: [Microscopy] TEM of functionalized nanoparticles
}
}
}
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}
} In recent years we have been getting requests to image functionalized
} nanoparticles, such as 10 nm gold with proteins or peptides attached.
} Generally the clients want to know how thick or large the organic
} "shell" is, or how the protein/peptide is arranged on the surface.
} Most of our experience has been with either inorganic particles (which
} we view without staining) or larger organic structures such as viral
} procapsids or large proteins (e.g., myosin) or protein assemblies,
} which we negative stain. My questions for this list are as follows:
}
} - have others on the list had good luck imaging these kinds of
} conjugates at the EM level, and if so, using what techniques?
} - how much organic material must one have associated with the
} nanoparticle to see it by negative staining or other methods? For
} example, how hard is it to detect a 150 kD protein on a 10 nm gold
} particle? An example would be IgG attached to a 10 nm gold particle.
} - can you suggest any publications or review articles on the subject?
} For some reason I'm not having much luck with my Pubmed search
} strategies. A recent paper by Cao and Mao seems to deal mainly with
} small gold particles on much larger protein aggregates, but perhaps
} there are others out there.
}
} Many thanks (in advance) for your suggestions.
}
} Dr. Marie E. Cantino
} Associate Professor of Physiology and Neurobiology Director, Electron
} Microscopy Laboratory University of Connecticut, Unit 3242
} Phone: 860-486-3588
} Fax: 860-486-6369
}
}
} ==============================Original
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} Stephane Nizet {nizets2-at-yahoo.com} 15, 42 -- Reply-To: Stephane Nizet
} {nizets2-at-yahoo.com} 15, 42 -- Subject: Re: [Microscopy] TEM of
} functionalized nanoparticles 15, 42 -- To: "marie.cantino-at-uconn.edu"
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Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology Director, Electron Microscopy Laboratory University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369


==============================Original Headers==============================
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18, 34 -- From cdh1-at-cdc.gov Mon Feb 6 09:21:14 2012
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From: vray-at-partbeamsystech.com
Date: Mon, 6 Feb 2012 09:46:38 -0600
Subject: [Microscopy] Re: viaWWW:TEM : Focusing Problem.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Image moving while focusing means that electron beam is not passing
through the optical center of the lens. Either there is a problem with
alignment electronics and it is pushed beam aside from its optimal path,
or there is a problem with electron-optical elements inside of the
column (maybe something got knocked out of alignment, or there is a
large particle that got charged and distorted the field, or something
else of similar nature).

In any case - look like you need to call a serviceman...

Valery Ray
=================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844 USA
Phone: +1-978-296-5063 - leave a message with call-back number
US Mobile: +1-978-305-0479
Skype: pbstmeo
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 2/6/2012 9:08 AM, microscopylistserver-noreply-at-microscopy.com wrote:
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} Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar
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} Title-Subject: [Filtered] TEM : Focusing Problem.
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} Message: Image is not getting focused. The image is drastically moving
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From: rok210-at-lehigh.edu
Date: Mon, 6 Feb 2012 10:05:31 -0600
Subject: [Microscopy] viaWWW:TEM : Focusing Problem.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

One thing to look for in the electronics readout system (perhaps a
computer with memory) is the alignment numbers, giving gun alignment
numbers (two sets for X and two sets of Y) and the condenser alignment
(likewise) and perhaps Projector alignment, even image shift on some
models. Basically you would love to have a set of "known good" numbers
for reference. By comparing the last known good alignment numbers with
the present numbers you can eliminate the possibility of the previous
user being rather too keen to perform 'dark field' experiments.

As previous posters mention it is the condenser lens (CL) TILT alignment
that might be in error, another possibility is that the sample is bent
horribly and the lens is focusing at a value that is nowhere near it's
optimal value (have you checked the eucentric height?). Again having
values of lens currents recorded after a good session can be useful and
many service people do just that, by checking the performance report
from the installation.

Is the Gun tilt (filament image), condenser aperture centering, and spot
size adjustments all perfectly normal?

Good luck,
Rob Keyse

On 2/6/2012 10:53 AM, vray-at-partbeamsystech.com wrote:
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} Image moving while focusing means that electron beam is not passing
} through the optical center of the lens. Either there is a problem with
} alignment electronics and it is pushed beam aside from its optimal path,
} or there is a problem with electron-optical elements inside of the
} column (maybe something got knocked out of alignment, or there is a
} large particle that got charged and distorted the field, or something
} else of similar nature).
}
} In any case - look like you need to call a serviceman...
}
} Valery Ray
} =================================
} PBS&T, MEO Engineering Co., Inc.
} 290 Broadway, Suite 298
} Methuen, MA 01844 USA
} Phone: +1-978-296-5063 - leave a message with call-back number
} US Mobile: +1-978-305-0479
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} Web: www.freudlabs.com
}
} On 2/6/2012 9:08 AM, microscopylistserver-noreply-at-microscopy.com wrote:
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} } This Question/Comment was submitted to the Microscopy Listserver
} } using the WWW based Form at
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} } replying please copy both ravi.thakkar369-at-gmail.com as well as the
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} }
} } Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar
} }
} } Title-Subject: [Filtered] TEM : Focusing Problem.
} }
} } Message: Image is not getting focused. The image is drastically moving
} } while focusing, it moving highly even at low focus step& at low
} } magnification.
} }
} } So how to resolve this problem.
} }
} } Login Host: 14.139.97.76
} } ---------------------------------------------------------------------------
} }

--
Robert Keyse
EM Facility
Whitaker Laboratory
5 East Packer Avenue
Bethlehem
PA 18015
USA

Tel. +1 610 758 4283
Fax +1 610 758 4244


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From: benada-at-biomed.cas.cz
Date: Mon, 6 Feb 2012 10:33:22 -0600
Subject: [Microscopy] TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Marie,
I agree with Charles: "you should be able to see IgG .... by negative
staining". However, I have never used negative staining for samples containing
colloidal gold particles bigger than 10 nm.
In my hand (~5 to ~10 nm gold particles on thin carbon support film), 1% or 2%
ammonium molybdate or its mixture with 0.1% trehalose worked better than
classic 2% uranyl acetate. Meniscus of uranyl acetate around the gold particle
was usually too dense to see IgG attached to it at 80 kV and tungsten cathode.

Best regards Oldrich

Prague
Czech Republic

On Monday 06 of February 2012 16:23:06 you wrote:
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} Hello Marie,
}
} I am not certain this answers your question but you should be able to
} detect IgG attached to an electron dense gold particle by negative stain
} EM. Antibodies are commonly seen attached to virus particles and to
} colloidal gold particles when negative stain EM is done. Anything you
} would likely see in your indicated study by AFM can be seen by negative
} stain EM. All techniques have various artifacts including light-scatter,
} thin-section EM, AFM, FESEM, and cryo-EM. The key to EM and techniques in
} general is to learn what is real and what is artifact. It often takes
} time and work to develop proficiency with many techniques though. Good
} luck with this , it should be possible.
}
} Charles Humphrey, Ph. D.
} CDC
} Atlanta, GA 30333
}
} -----Original Message-----
} X-from: marie.cantino-at-uconn.edu [mailto:marie.cantino-at-uconn.edu]
} Sent: Monday, February 06, 2012 9:49 AM
} To: Humphrey, Charles (CDC/OID/NCEZID)
} Subject: [Microscopy] TEM of functionalized nanoparticles
}
}
}
}
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} America To Subscribe/Unsubscribe --
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}
} Hi Stefan,
}
} I do understand the difference between mass and length.
}
} The problem is that the clients usually can only give us the molecular
} weight and (if we're lucky) the number of molecules per particle. If they
} knew how the proteins/peptides were arranged or the linear dimensions of
} the coating they wouldn't need to come to me. What I'm trying to find out
} from the listserver or the literature is whether we can reasonably expect
} to be able to detect a medium sized protein such as IgG on an electron
} dense gold particle, given the various artifacts of techniques such as
} negative staining.
}
} We only have TEM (w/a tungsten emitter) and FESEM in our facility, but I
} can send them elsewhere for AFM.
}
} Marie
}
} On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote:
} } ----------------------------------------------------------------------
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} }
} } Hi Marie,
} }
} } I like these projects which put the techniques to their limits.
} } I think the reason why you have difficulties finding publications on
} } this matter and that the question is not trivial at all.
} } First of all, and to start with a relaxed note, the mixing of kDa und
} } nm in one sentence made me smile because it makes as much sense as
} } asking if 1kg of something fits in one meter.
} } The Dalton is a unit of mass and does not give a good indication of
} } the dimension or the shape of a molecule.
} } That said, I seem to remember from the past answers of our emminent
} } immunoglobulinologist Jan Leunissen that the size of an IgG is several
} } nm, I think more than 5nm.
} } Which means, in your case, that you should not expect more that a few
} } molecules per nanoparticle, except if they form multilayers.
} } As for the techniques, I wonder if a SEM could resolve this structure.
} } Although I have experience with analytical low res SEMs, I don't know
} } the limits of modern FEG SEMs.
} } I would be happy if some listers would care to share their opinion on
} } this matter.
} } It seems that AFM (atomic force microscopy) would be able to resolve
} } this structure, but I wonder if it would be possible in this condition
} } because AFM works on flat substrate and here you have round particles.
} } It would probably be possible to find some answers using cry-
} } microscopy, the same way scientists use it to resolve viral structures
} } at several anstrom resolution. It probably depends on how much time
} } and money your clients are wanting to spend and how critical this
} } information is for them.
} }
} } Regards,
} } Stephane
} }
} } ----- Original Message -----
} } X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu}
} } To: nizets2-at-yahoo.com
} } Cc:
} } Sent: Friday, February 3, 2012 8:10 PM
} } Subject: [Microscopy] TEM of functionalized nanoparticles
} }
} }
} }
} }
} } ----------------------------------------------------------------------
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} } of America To Subscribe/Unsubscribe --
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} } ----------------------------------------------------------------------
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} }
} } In recent years we have been getting requests to image functionalized
} } nanoparticles, such as 10 nm gold with proteins or peptides attached.
} } Generally the clients want to know how thick or large the organic
} } "shell" is, or how the protein/peptide is arranged on the surface.
} } Most of our experience has been with either inorganic particles (which
} } we view without staining) or larger organic structures such as viral
} } procapsids or large proteins (e.g., myosin) or protein assemblies,
} } which we negative stain. My questions for this list are as follows:
} }
} } - have others on the list had good luck imaging these kinds of
} } conjugates at the EM level, and if so, using what techniques?
} } - how much organic material must one have associated with the
} } nanoparticle to see it by negative staining or other methods? For
} } example, how hard is it to detect a 150 kD protein on a 10 nm gold
} } particle? An example would be IgG attached to a 10 nm gold particle.
} } - can you suggest any publications or review articles on the subject?
} } For some reason I'm not having much luck with my Pubmed search
} } strategies. A recent paper by Cao and Mao seems to deal mainly with
} } small gold particles on much larger protein aggregates, but perhaps
} } there are others out there.
} }
} } Many thanks (in advance) for your suggestions.
} }
} } Dr. Marie E. Cantino
} } Associate Professor of Physiology and Neurobiology Director, Electron
} } Microscopy Laboratory University of Connecticut, Unit 3242
} } Phone: 860-486-3588
} } Fax: 860-486-6369
} }
} }
} } ==============================Original
} } Headers==============================
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} } {nizets2-at-yahoo.com} 15, 42 -- Subject: Re: [Microscopy] TEM of
} } functionalized nanoparticles 15, 42 -- To: "marie.cantino-at-uconn.edu"
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}
} Dr. Marie E. Cantino
} Associate Professor of Physiology and Neurobiology Director, Electron
} Microscopy Laboratory University of Connecticut, Unit 3242 Phone:
} 860-486-3588
} Fax: 860-486-6369
}
}
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From: W.Muss-at-salk.at
Date: Mon, 6 Feb 2012 11:34:16 -0600
Subject: [Microscopy] Re: Counting virus particles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

} -----UrsprŁngliche Nachricht-----
} Von: MuŖ Wolfgang
} Gesendet: Montag, 06. Februar 2012 11:49
} An: 'mary.walker-at-csl.com.au'
} Cc: microscopy-at-microscopy.com
} Betreff: [Microscopy] Re: Counting virus particles

Dear Mary,
Greetings to Melbourne/Australia !

Hopefully you are not situated in the area of floodings!


Concerning your question(s) below I would like to point you to a MSA-
Listserver-thread (2007) with -/+ such background (a compilation of the
postings I could send to you relatively fast...but there would be also
other interesting questions and answers (which one can find also in
recent and a little bit "older" specific literature (from experts) on
that matter.
So, please, if you would like to get a compilation of the 2007-MSA-LS-
thread (instead of searching for them in the archives) please let me
know (and "allow" for sending to your mailbox...),


for now, best regards,
Wolfgang MUSS

} SALZBURG, AUSTRIA
}
} } -----UrsprŁngliche Nachricht-----
} } Von: microscopylistserver-noreply-at-microscopy.com
} } [mailto:microscopylistserver-noreply-at-microscopy.com]
} } Gesendet: Freitag, 03. Februar 2012 16:40
} } An: MuŖ Wolfgang
} } Betreff: [Microscopy] Counting virus particles
}
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} } Email: mary.walker-at-csl.com.au
} } Name: mary walker
} } Organization: csl limited
} } Title-Subject: counting virus particles
} } Message:
} } Do any listers have experience with counting virus particles with
} latex
} } beads?
} } Is it possible to do better than order of magnitude?
} } What is the minimum number of particles per ml that can be counted?
} } What is the lower limit?
} }
} } thanks in advance
} }
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From: microscopylistserver-noreply-at-microscopy.com
Date: Mon, 6 Feb 2012 13:59:24 -0600
Subject: [Microscopy] viaWWW:Service Manual_Zeiss EM-906

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
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Email: csrayat-at-gmail.com Name: Dr CS Rayat

Organization: PGIMER, Chandigarh, India

Title-Subject: [Filtered] Service Manual_Zeiss EM-906

Message: Hi Friends !

Please e-mail me a copy (pdf format) of Service Manual of Zeiss EM-906
(Transmission Electron Microscope) or Trouble Shooting Manual for the same.
Best regards,

CS Rayat



Login Host: 59.94.236.178
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From: leunissen-at-aurion.nl
Date: Mon, 6 Feb 2012 16:25:05 -0600
Subject: [Microscopy] Re: TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Marie,

Somewhere in the 90's I believe I read an abstract that showed excellent negative staining of gold particles attached to immunoglobulin. Unfortunately, I do not have access to that literature here so I can not help you with a reference. If I remember correctly in those studies first the Ig component was a adsorbed onto a grid film which was then, after rinsing incubated with the gold particles to study the way those particles bound and negatively stained. I believe these were proceedings from one of the international EM conferences, perhaps someone else has access?

Good luck,

Jan Leunissen

e: http://www.aurion.nl


On 4/02/2012, at 8:07 AM, marie.cantino-at-uconn.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
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} In recent years we have been getting requests to image functionalized
} nanoparticles, such as 10 nm gold with proteins or peptides attached.
} Generally the clients want to know how thick or large the organic
} "shell" is, or how the protein/peptide is arranged on the surface.
} Most of our experience has been with either inorganic particles (which
} we view without staining) or larger organic structures such as viral
} procapsids or large proteins (e.g., myosin) or protein assemblies,
} which we negative stain. My questions for this list are as follows:
}
} - have others on the list had good luck imaging these kinds of
} conjugates at the EM level, and if so, using what techniques?
} - how much organic material must one have associated with the
} nanoparticle to see it by negative staining or other methods? For
} example, how hard is it to detect a 150 kD protein on a 10 nm gold
} particle? An example would be IgG attached to a 10 nm gold particle.
} - can you suggest any publications or review articles on the subject?
} For some reason I'm not having much luck with my Pubmed search
} strategies. A recent paper by Cao and Mao seems to deal mainly with
} small gold particles on much larger protein aggregates, but perhaps
} there are others out there.
}
} Many thanks (in advance) for your suggestions.
}
} Dr. Marie E. Cantino
} Associate Professor of Physiology and Neurobiology
} Director, Electron Microscopy Laboratory
} University of Connecticut, Unit 3242
} Phone: 860-486-3588
} Fax: 860-486-6369
}
}
} ==============================Original Headers==============================
} 5, 18 -- From marie.cantino-at-uconn.edu Fri Feb 3 13:07:15 2012
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} 5, 18 -- Subject: TEM of functionalized nanoparticles
} 5, 18 -- Date: Fri, 3 Feb 2012 14:07:15 -0500
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From: bozzola-at-siu.edu
Date: Mon, 6 Feb 2012 17:15:36 -0600
Subject: [Microscopy] Re: New management topic - discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I was also amused by the "driving test" analogy and really smiling at
Steve Chapman's response.

I thought the old, Siemens Elmiskop had an great approach to an
operational mistake: a red bar would loudly clunk down into place, in
essence, screaming at the operator, "You made a BIG mistake, here!
Call someone who knows what they are doing." I know someone who nearly
fainted when this occurred (in the wee hours of the morning with no
one else in the building). I never had the chance to use the
instrument, though I really admired the high resolution images from
this excellent instrument.

--

John J. Bozzola, Ph.D.
Gleefully Retired Professor & Director of IMAGE
Integrated Microscopy & Graphics Expertise
Southern Illinois University
Carbondale, IL  62901

On Mon, Feb 6, 2012 at 4:56 PM, {protrain-at-emcourses.com} wrote:
} Hi All
}
} I am always amused when people talk about giving users a driving test before allowing them to use a microscope. Not only do I teach people to use electron microscopes I teach people to drive racing vehicles and it is only then that, with a smile, you realise just how much a driving test on a microscope differs dramatically from any other type of "driving test"!  When you drive a car or any other vehicle and make an error you frighten yourself and perhaps worry that you will be hurt, and you do not make the same mistake again.  Contrast this when there is no pain from making an error on a microscope.  The best computing system that I have seen that trained people correctly was MS Dos and MS Word One, here make any error and you were faced with a screen stating "fatal error", the machine would then crash and all would be lost.  You learned pretty quickly or just gave up!
}
} Yes people should undergo a microscope "driving test".  The test should be designed to produce a result that clearly demonstrates that the operator took into account every pertinent feature of the instrument.  From my experience a number of specimens are required each demanding an understanding of different features within the instrument.  I am often forced to say, when evaluating laboratories and their staff, you may have 1nm instruments but do you realise you have 5nm staff?  Staff testing in my mind is required and through repeated testing and under pressure the staff will develop their skills!
}
} Think of "Quality" the Japanese way - repeated evaluation and improvement.
}
} Some ramblings of an electron microscopist clocking up 48 years in the business.
}
} Steve
}
} Steve Chapman FRMS
} Senior Consultant Protrain
} For consultancy and training in electron microscopy world wide
} Tel +44 (0)1280 816512  Fax +44 (0)1280 814007 Cell +44 (0)7711 606967
} www.emcourses.com
}
} -----Original Message-----
} From: M. Redigolo [mailto:marcela.redigolo-at-mail.wvu.edu]
} Sent: 06 February 2012 17:53
} To: Allan Mitchell; Andrew D. Hollingsworth; Andrew Sullivan; Hands-Portman Ian; j.janssen-at-nki.nl; John Bozzola; Moore Jayma; Naomi McCallum; Patricia Nelson; protrain-at-emcourses.com; Richard E. Edelmann; sallystowe-at-gmail.com; Sherman Debra; Straszheim Warren E [BIOTC]; tommibarhorst-at-gmail.com; vray-at-partbeamsystech.com; Wim Hagen; Wim Van Den Broeck
} Subject: New management topic - discussion
}
} Dear all:
}
} During the benchmarking this past week, two topics came up that it seems
} many want to discuss.
} So, I'm putting the topics out there to know what is the common practice in
} other facilities.
}
} a) How to evaluate the efficiency (in terms of results, quality of data,
} budget, etc) of management, fees and users?
} b) Service contracts - self-service / all-inclusive / OEM service contract /
} labor-only OEM service contract / third-party
} service contract / per-hour paid service from OEM or third-party /
} self-service with OEM support or with third-party support?
}
} Any inputs on those topics?
}
} Currently here, we have full service contract (all-inclusive) for both JEOL
} instruments. In the case of the SEM, 5 years (3 years to go)
} and for the TEM, we pay it yearly. This is one point that I might review and
} change if I know that other model can also work fine and
} save us money.
}
} We do collect a copy of every publication resulted from using the facilities
}  Users are very good in sending us these copies since we use
} them to justify increase of budget. Also, every user is requested to add an
} acknowledgement line mentioning the facilities, in every publication.
} Besides that, fees are reviewed yearly as I commented in the previous email.
} In terms of usage of the instruments, in some cases (as it is for the TEM)
} users undergo a "driver's test" before becoming unassisted users.
}
} OK. I promise I'll stop here and not crowd your inbox with long emails...
}
} Thanks again!
}
} Marcela.
}
}
} ÔĽŅ ÔĽŅ ÔĽŅ ÔĽŅ ÔĽŅ ÔĽŅ
} ------
} Dr. Marcela Redigolo
} Electron Microscopy Facility
} WVU Shared Research Facilities
} (304) 680-3007
} marcela.redigolo-at-mail.wvu.edu
}
}
}
}


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From: r-holdford-at-ti.com
Date: Mon, 6 Feb 2012 19:05:40 -0600
Subject: [Microscopy] Texas Society for Microscopy Spring Meeting Apr. 12-14 at TCU in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Texas Society for Microscopy invites you to participate in
its 2012 meeting taking place on the TCU campus Apr. 12-14. The
meeting location is the Brown-Lupton University Union while the
host hotel will be the SpringHill Suites by Marriott University
Fort Worth. Shuttle service from the hotel to campus will be
available. The abstract deadline is March 1. Additional meeting
information and forms can be found on our website:
http://www.texasmicroscopy.org.

Workshops scheduled for Thursday, April 12th, 1 Ė 4 PM
Care & Feeding of Your Light Microscope and Light Source
Sample Preparation Techniques for Materials Science Ė Choosing
the Right Method by Robert Ranner, Product Manager, Leica
Applications Specialist
Etching, ion beam milling, slope cutting Ė whatís the difference?
Target surface preparation Ė getting it down to size.
Ultrasectioning Ė why go cryo?
Hands-on demonstration:
TXP Ė multi-functional target preparation device which can saw,
mill, grind and polish
TIC3X Ė automated ion beam milling system
UC7/FC7 Ė advanced room and cryo temperature ultramicrotome

Invited Speakers Friday, April 13, 2012
Dr. Ray H. Baughman, Alan G. MacDiarmid NanoTech Institute, The
University of Texas at Dallas
- Biscrolling Nanofiber Sheets and Functional Guests into
Multifunctional Yarns for Energy Applications

Dr. Aydogan Ozcan, Bio- and Nano-Photonics Laboratory, UCLA
Ė Photonics-based Telemedicine Technologies Toward Smart Global
Health Systems

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From: dsherman-at-purdue.edu
Date: Mon, 6 Feb 2012 19:46:52 -0600
Subject: [Microscopy] New management topic - discussion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Ah, fond memories. My first real experience in TEM (besides playing with a
Philips 75 as an undergrad) was in the lab of Humberto Fernandez-Moran at
the University of Chicago. We had Siemens Elmiskop 1 and 1a microscopes.
Oh yes...saw that red bar on numerous occasions. The scope had a mechanical
valve block and if it was not turned properly the bar sprang up. Made enough
noise so those in the next room knew that you did a no-no.

However, I still have glass negatives of some images taken from that
instrument. I have never seen better coming from any modern W-filament
instrument. Check out the T4 phage (at www.dsimagingllc.com under "Images }
animal"), which was taken 45 years ago when I had been using the Siemens
Elmiskop I for about 6 months. Before being able to look at real sample, we
had to produce a perfect objective stigmation series on a hole sample at
very high mag, using the mechanical stigmator system available in those
days. I really learned how to stigmate and focus incredibly well with almost
no illumination. It has been a skill that I still value extremely highly.
Spent a lot of time in the dark working on that microscope along with a
young grad student who became my husband. There is a lot to be said for
spending time in the dark! One big advantage we had was using what I
believe were the first pointed filaments that were made in the lab. But that
is another story. For those calculating my age, I was very young at the time
to have access to such an instrument.....

Debby Sherman



On 2/6/12 6:16 PM, "John Bozzola, Phd. Director" {bozzola-at-siu.edu} wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} I was also amused by the "driving test" analogy and really smiling at
} Steve Chapman's response.
}
} I thought the old, Siemens Elmiskop had an great approach to an
} operational mistake: a red bar would loudly clunk down into place, in
} essence, screaming at the operator, "You made a BIG mistake, here!
} Call someone who knows what they are doing." I know someone who nearly
} fainted when this occurred (in the wee hours of the morning with no
} one else in the building). I never had the chance to use the
} instrument, though I really admired the high resolution images from
} this excellent instrument.



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9, 31 -- {microscopy-at-microscopy.com}
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From: forzaabbott-at-gmail.com
Date: Mon, 6 Feb 2012 23:43:07 -0600
Subject: [Microscopy] Re: TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I imagine you would have difficulty using AFM if you needed to count things attached to particles. AFM gives you a 2D projection of the sample, you would miss anything under or "shadowed" by the particle or anything else. AFM doesn't do reentrant profiles (typically).
If all you need is to see if something's attached, get some size information (caution about reentrant profiles again), and you have well characterized particles to attach to, AFM might be able to help you.

Jonathan Abbott

On Feb 6, 2012, at 7:48, marie.cantino-at-uconn.edu wrote:

}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Stefan,
}
} I do understand the difference between mass and length.
}
} The problem is that the clients usually can only give us the molecular
} weight and (if we're lucky) the number of molecules per particle. If
} they knew how the proteins/peptides were arranged or the linear
} dimensions of the coating they wouldn't need to come to me. What I'm
} trying to find out from the listserver or the literature is whether we
} can reasonably expect to be able to detect a medium sized protein such
} as IgG on an electron dense gold particle, given the various artifacts
} of techniques such as negative staining.
}
} We only have TEM (w/a tungsten emitter) and FESEM in our facility, but
} I can send them elsewhere for AFM.
}
} Marie
}
} On Feb 6, 2012, at 5:33 AM, nizets2-at-yahoo.com wrote:
}
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi Marie,
} }
} } I like these projects which put the techniques to their limits.
} } I think the reason why you have difficulties finding publications on
} } this matter and that the question is not trivial at all.
} } First of all, and to start with a relaxed note, the mixing of kDa
} } und nm in one sentence made me smile because it makes as much sense
} } as asking if 1kg of something fits in one meter.
} } The Dalton is a unit of mass and does not give a good indication of
} } the dimension or the shape of a molecule.
} } That said, I seem to remember from the past answers of our emminent
} } immunoglobulinologist Jan Leunissen that the size of an IgG is
} } several nm, I think more than 5nm.
} } Which means, in your case, that you should not expect more that a
} } few molecules per nanoparticle, except if they form multilayers.
} } As for the techniques, I wonder if a SEM could resolve this
} } structure. Although I have experience with analytical low res SEMs,
} } I don't know the limits of modern FEG SEMs.
} } I would be happy if some listers would care to share their opinion
} } on this matter.
} } It seems that AFM (atomic force microscopy) would be able to resolve
} } this structure, but I wonder if it would be possible in this
} } condition because AFM works on flat substrate and here you have
} } round particles.
} } It would probably be possible to find some answers using cry-
} } microscopy, the same way scientists use it to resolve viral
} } structures at several anstrom resolution. It probably depends on how
} } much time and money your clients are wanting to spend and how
} } critical this information is for them.
} }
} } Regards,
} } Stephane
} }
} } ----- Original Message -----
} } X-from: "marie.cantino-at-uconn.edu" {marie.cantino-at-uconn.edu}
} } To: nizets2-at-yahoo.com
} } Cc:
} } Sent: Friday, February 3, 2012 8:10 PM
} } Subject: [Microscopy] TEM of functionalized nanoparticles
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} } America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } In recent years we have been getting requests to image functionalized
} } nanoparticles, such as 10 nm gold with proteins or peptides attached.
} } Generally the clients want to know how thick or large the organic
} } "shell" is, or how the protein/peptide is arranged on the surface.
} } Most of our experience has been with either inorganic particles (which
} } we view without staining) or larger organic structures such as viral
} } procapsids or large proteins (e.g., myosin) or protein assemblies,
} } which we negative stain. My questions for this list are as follows:
} }
} } - have others on the list had good luck imaging these kinds of
} } conjugates at the EM level, and if so, using what techniques?
} } - how much organic material must one have associated with the
} } nanoparticle to see it by negative staining or other methods? For
} } example, how hard is it to detect a 150 kD protein on a 10 nm gold
} } particle? An example would be IgG attached to a 10 nm gold particle.
} } - can you suggest any publications or review articles on the subject?
} } For some reason I'm not having much luck with my Pubmed search
} } strategies. A recent paper by Cao and Mao seems to deal mainly with
} } small gold particles on much larger protein aggregates, but perhaps
} } there are others out there.
} }
} } Many thanks (in advance) for your suggestions.
} }
} } Dr. Marie E. Cantino
} } Associate Professor of Physiology and Neurobiology
} } Director, Electron Microscopy Laboratory
} } University of Connecticut, Unit 3242
} } Phone: 860-486-3588
} } Fax: 860-486-6369
} }
} }
} } ==============================Original
} } Headers==============================
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} } 5, 18 -- From: Marie Cantino {marie.cantino-at-uconn.edu}
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From: S.Walck-at-cox.net
Date: Tue, 7 Feb 2012 02:10:42 -0600
Subject: [Microscopy] image shift on CM20

Contents Retrieved from Microscopy Listserver Archives
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I had a session on a Philips CM20 today.† I was working at relatively high
mag and I wanted to shift the image instead of using the mechanical stage
shifts.† For the life of me, I could not figure out how to do it.† Iím not
that familiar with the instrument, but Iím sure that it can be done.† Can
anyone tell me the controls for it?


-Scott

Scott D. Walck, Ph.D.
5234 Sandalwood PL
Oceanside, CA 92056

S.Walck-at-cox.net
SWalck65-at-gmail.com

(760) 685-2815 (Cell)
(760) 758-4384 (Home)




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From: reinhard.rachel-at-biologie.uni-regensburg.de
Date: Tue, 7 Feb 2012 03:10:45 -0600
Subject: [Microscopy] TEM / AFM and nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

let me briefly comment on one sentence in Jonathan's comment from yesterday.

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 2837, 1720
fax +49 941 943 2868
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29

next microscopy conferences:
http://www.emc2012.org.uk/
EMC 2012 in Manchester, UK
http://www.mc2013.de/
MC2013 in Regensburg, Germany


} } }
} I imagine you would have difficulty using AFM if you needed to count things
} attached to particles.
} AFM gives you a 2D projection of the sample,

no, this is not the case. AFM will give you an image representing the surface / surface relief of the sample, but not a 2D projection (in the sense as the TEM does). You will not get any information of the internal parts of the sample.

} you would miss anything under or "shadowed" by the particle or anything else. AFM
} doesn't do reentrant profiles (typically).

} If all you need is to see if something's attached, get some size information
} (caution about reentrant profiles again), and you have well characterized
} particles to attach to, AFM might be able to help you.

here, I fully agree.
Kind regards,
Reinhard




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From: microscopylistserver-noreply-at-microscopy.com
Date: Tue, 7 Feb 2012 04:30:50 -0600
Subject: [Microscopy] viaWWW:Diagonal bands in STEM mode

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Email: amit.welcomes.u-at-gmail.com Name: Amit

Organization: Indian institute of science

Title-Subject: [Filtered] Diagonal bands in STEM mode

Message: we recently installed JEOL JEM-2100F microscope. rest is
working fine but in STEM mode there are Diagonal Bands of alternate
brightness coming(all bands or strips are of same thickness, brightness
and contrast). on increasing scan time band's thickness first increases
then at even higher scan times it decreases. Engineers are on it but
cant pin point the fault. i was wondering if anyone encountered it
before or knows why this is happening

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From: mike.bode-at-resaltatech.com
Date: Tue, 7 Feb 2012 10:28:04 -0600
Subject: [Microscopy] viaWWW:Diagonal bands in STEM mode

Contents Retrieved from Microscopy Listserver Archives
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Hello Amit,

"stripes" in images are often the result of interference. It often helps to
determine the frequency to eliminate sources (for example if the frequency
is 50 or 60 Hz, it usually comes from a power source). Have you tried to
determine the frequency?

Mike Bode, Ph.D.
ResAlta Research Technologies Corp.

2102 Beech Ct
Golden, CO 80401
USA

p: +1-303-234-9270
f: +1-303-202-6350
Mike.Bode-at-ResAltaTech.com
www.ResAltaTech.com





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Email: amit.welcomes.u-at-gmail.com Name: Amit

Organization: Indian institute of science

Title-Subject: [Filtered] Diagonal bands in STEM mode

Message: we recently installed JEOL JEM-2100F microscope. rest is
working fine but in STEM mode there are Diagonal Bands of alternate
brightness coming(all bands or strips are of same thickness, brightness
and contrast). on increasing scan time band's thickness first increases
then at even higher scan times it decreases. Engineers are on it but
cant pin point the fault. i was wondering if anyone encountered it
before or knows why this is happening

Login Host: 14.139.128.11
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From: petereaton-at-hotmail.com
Date: Tue, 7 Feb 2012 11:30:57 -0600
Subject: [Microscopy] TEM/AFM and nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



Regarding†AFM of gold nanoparticles with proteins attached, I have some experience with†this.

As was†mentioned, AFM (high resolution at least) works best with flat samples. You can
certainly images round particles with no difficulty, but you will not normally†get many details. What you will see is a ďblobĒ, most likely. Now, if your†particles are nice (i.e. close to monodisperse), and your protein size is†significant compared to your gold particle size, what you can do, is compare the†ďunfunctionalisedĒ size (height of the blob) to the functionalised size, and†use this difference as a measure of how much, if any, protein got attached. AFM†has very good resolution in height, getting images good enough to make these†measurements would be simple. The limiting factor here will most likely be,†what is the significance of your height measurements, given the range of height†of the ďbareĒ particles. Also, how many AFM images you can bear to measure, to†get† a significant sample.



By the way,†no, we donít directly compare kDa and nanometres, but I understood exactly why†the poster said this...ask a biochemist how big their protein is, and they will always reply in terms of Daltons. We can, however make a *guess* at the size based on the†height, and I can tell you that your 150kDa particle WILL make a significant difference†to the 10 nm AuNPīs diameter, since I have done this with much smaller proteins.



On the†other hand, there is no doubt that if the negative staining works well, and you†can image both the the AuNPs and proteins, it will be a nicer result, and probably less†time consuming.



Good Luck!

Pete.


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From: AJBowling-at-dow.com
Date: Tue, 7 Feb 2012 14:57:10 -0600
Subject: [Microscopy] RE: TEM/AFM and nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thank you, Wim. I believe this is what I want. I didn't use the HR-TEM
mode, I used the TEM mode. Wim Hagen's direct email message to me included
a readout image of the HR-TEM page and it shows the Image Shift as well as
some other HR-TEM functions. His answer was to use the HR-TEM mode, choose
Parameter page and the appropriate control pages come up.

Chad Parish, John Minter, and Reinhard Rachel suggested using the
Alignment-Image Shift. I did try that and all it did when I touched the
multi-knobs was to beep at me. John said that this function is for keeping
the different mags aligned.

Thank you all for your help. Next time I'm on the microscope, I'll try it.



-Scott

Scott D. Walck, Ph.D.
5234 Sandalwood PL
Oceanside, CA 92056

S.Walck-at-cox.net
SWalck65-at-gmail.com

(760) 685-2815 (Cell)
(760) 758-4384 (Home)


-----Original Message-----
X-from: Wim Hagen [mailto:wim.hagen-at-me.com]
Sent: Tuesday, February 07, 2012 12:28 AM
To: S.Walck-at-cox.net

Another thing you could try is high-angle Pt/C shadowing.

I don't know if it's easier to find an AFM or a working high vacuum evaporator these days, though!

Andy Bowling




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From: nizets2-at-yahoo.com
Date: Wed, 8 Feb 2012 04:18:05 -0600
Subject: [Microscopy] TEM/AFM and nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I already gave my opinion to Marie about this offline but since there were several answers in the list going in this direction I would like to share some thoughts with the list.

Negative staining may work. This means: you may see your proteins, you may get a result. You may even get a nice picture.
But hey, are we artists or are we scientists?
Our job is not to get nice pictures, it is to get meaningful pictures.

Marie wants to know how the antibodies (or other proteins) are organized around gold particles in a matrix, most probably water or aqueous solution.
I am not sure the best way to know that is by drying the sample with a heavy stain.
Do you really think it may not interfere with the binding forces between the antibody and the gold particle? I wouldn't bet a cent on it!

Regards,
Stephane

----- Original Message -----
X-from: "petereaton-at-hotmail.com" {petereaton-at-hotmail.com}
To: nizets2-at-yahoo.com
Cc:
Sent: Tuesday, February 7, 2012 6:33 PM




On the†other hand, there is no doubt that if the negative staining works well, and you†can image both the the AuNPs and proteins, it will be a nicer result, and probably less†time consuming.



Good Luck!

Pete.
††† ††† ††† † ††† ††† † ††† ††† ††† † ††† ††† †

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From: cdh1-at-cdc.gov
Date: Wed, 8 Feb 2012 08:33:48 -0600
Subject: [Microscopy] TEM/AFM and nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Stephane,

This conundrum you indicate is like life. One picks his or her poison according to the needs, goals, facilities available, time, and economic/collaborative capabilities. Sometimes an association is all the information that is needed to answer a question. All of the methods available today for visualizing proteins have flaws. Negative stain EM is a good first start with other techniques such as cryo-EM and AFM later based on what was learned by "crude" negative stain EM, if indeed one is attempting to study how proteins are organized around a matrix.

Best regards,

Charles Humphrey, Ph.D.
CDC
Atlanta, GA 30333


-----Original Message-----
X-from: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Sent: Wednesday, February 08, 2012 5:28 AM
To: Humphrey, Charles (CDC/OID/NCEZID)

I already gave my opinion to Marie about this offline but since there were several answers in the list going in this direction I would like to share some thoughts with the list.

Negative staining may work. This means: you may see your proteins, you may get a result. You may even get a nice picture.
But hey, are we artists or are we scientists?
Our job is not to get nice pictures, it is to get meaningful pictures.

Marie wants to know how the antibodies (or other proteins) are organized around gold particles in a matrix, most probably water or aqueous solution.
I am not sure the best way to know that is by drying the sample with a heavy stain.
Do you really think it may not interfere with the binding forces between the antibody and the gold particle? I wouldn't bet a cent on it!

Regards,
Stephane

----- Original Message -----
X-from: "petereaton-at-hotmail.com" {petereaton-at-hotmail.com}
To: nizets2-at-yahoo.com
Cc:
Sent: Tuesday, February 7, 2012 6:33 PM




On the†other hand, there is no doubt that if the negative staining works well, and you†can image both the the AuNPs and proteins, it will be a nicer result, and probably less†time consuming.



Good Luck!

Pete.
††† ††† ††† † ††† ††† † ††† ††† ††† † ††† ††† †

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From: nizets2-at-yahoo.com
Date: Wed, 8 Feb 2012 09:57:45 -0600
Subject: [Microscopy] X ray control after dismounting a TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello list!

We have taken almost all the shielding from our Tecnai G20 down in order to make some repairs and now that I am ready to mount them again, I ask myself:
Should I ask FEI to come and control the X-radiation?
We work mostly at 100-120kV but sometimes we use 200kV so X-rays should be considered, but is there a risk of leak after unmounting/remounting the shielding?
Thanks in advance for sharing your thoughts.

Regards,
Stephane


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From: marie.cantino-at-uconn.edu
Date: Wed, 8 Feb 2012 13:19:25 -0600
Subject: [Microscopy] RE: TEM of functionalized nanoparticles

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Thanks to everyone who has responded to my question. Since there
seemed to be quite a bit of interest, I will summarize what I've
discovered so far (I hope those who responded off-line will not mind
being paraphrased, and my apologies if I accidentally omitted your
suggestions. There were a lot of emails.)

Several people suggested publications, and I did additional searches
on my own (though not exhaustive). Google Scholar proved to be more
useful than Pubmed, perhaps because many of these papers are in
chemistry journals.

Here are some papers that include EM methods (noted in parentheses),
in no particular order:

Transmission Electron Microscopy as a Tool to Image Bioinorganic
Nanohybrids: The Case of Phage-Gold Nanocomposites MICROSCOPY RESEARCH
AND TECHNIQUE 74:627Ė635 (2011) (negative staining, mainly of larger
protein assemblies)

Apoferritin-Templated Synthesis of Encoded Metallic Phosphate
Nanoparticle Tags. Anal. Chem. 2007, 79, 5614-5619. (TEM unstained and
negative stained).

Direct Imaging of Soft-Hard Interfaces Enabled by Graphene. Nano
Letters 2009 Vol 9 No 9 3365-3369 (very cool images using high
resolution TEM and substrate, unstained)

In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid
Micelles. SCIENCE VOL 298 29 NOVEMBER 2002 1759-1762 (negative
stained)

Electron Density Imaging of Protein Films on Gold-Particle Surfaces
With Transmission Electron Microscopy. Ludger Ju®rgens,* Alfons
Nichtl, and Ulf Werner. Cytometry 37:87Ė92 (1999) (TEM, unstained and
ruthenium stained prior to conjugation)

Controlled Encapsidation of Gold Nanoparticles by a Viral Protein
Shell. LiNa Loo, Richard H. Guenther, Veronica R. Basnayake, Steven
A. Lommel, and Stefan Franzen*. J. AM. CHEM. SOC. 2006, 128,
4502-4503 (negative stain TEM and possibly cryo, but results not shown)

A fluorescent, paramagnetic and PEGylated gold/silica nanoparticle for
MRI, CT and fluorescence imaging Matti M. van Schoonevelda, David P.
Cormodeb, Rolf Koole, J. Timon van Wijngaardena, Claudia Calcagno,
Torjus Skajaa, Jan Hilhorst, Dannis C. ít Hartc, Zahi A. Fayadb,
Willem J. M. Mulder* and Andries Meijerinka. Contrast Media Mol.
Imaging 2010, 5 231Ė236 2010. (negative and positive stain)

DNA-Origami-Directed Self-Assembly of Discrete Silver-Nanoparticle
Architectures. Suchetan Pal, Zhengtao Deng, Baoquan Ding, Hao Yan,*
and Yan Liu. Angew. Chem. 2010, 122, 2760 Ė2764 (negative stain w/
uranyl formate and unstained with STEM; not typical gold
nanoparticles, but pretty interesting structures!)

New Frontiers in Gold Labeling. James F. Hainfeld and Richard D.
Powell The Journal of Histochemistry & Cytochemistry 48(4): 471Ė480,
2000 (STEM images of nanogold labelled Fab)

Electron microscopy localization and characterization of
functionalized composite organic-inorganic SERS nanoparticles on
leukemia cells Ai Leen Koh , Catherine M. Shachaf, Sailaja Elchuri,
Garry P. Nolan, Robert Sinclair. Ultramicroscopy 109 (2008) 111Ė121
(negative stain TEM, SEM, BSE, Quantomix SEM and EDS)

In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
Benoit Dubertret, Paris Skourides, David J. Norris, Vincent Noireaux,
Ali H. Brivanlou, Albert Libchaber. SCIENCE VOL 298 29 NOVEMBER2002
(negative stain TEM)

Methods suggested:

-Negative staining, Uranyl acetate (0.5-2%) or ammonium molybdate
(1-2% w/trahalose), preferably with FEG or LaB6. Use
unfunctionalized particles as a control. Use of trehalose or an
embedding medium such as methyl cellulose was also suggested (see
Methods in Molecular Biology 369 Electron Microscopy: Mehods and
Protocols, 2nd edition, Chapter 7 by Robin Harris). In general,
drying artifacts such as flattening are likely to be observed with
negative staining, but use of these embedments will reduce this.

-Use of graphene support films see paper above in Nano Letters.

-dynamic light scattering

-cryo TEM or cryo FESEM were suggested

-Pt/C shadowing was suggested

-AFM. The dimension of the coating can be estimated by comparing the
height of the coated and uncoated particles.


Dr. Marie E. Cantino
Associate Professor of Physiology and Neurobiology
Director, Electron Microscopy Laboratory
University of Connecticut, Unit 3242
Phone: 860-486-3588
Fax: 860-486-6369



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From: LettJ-at-ent.wustl.edu
Date: Wed, 8 Feb 2012 13:48:57 -0600
Subject: [Microscopy] SEM protein on grid or cover slip

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Our client wants us to image proteins using SEM. The protein is dissolved in water and currently stored at -20degrees C. What do you suggest? This is what I'm thinking:

Float a formvar-coated grid on drop of solution or place a drop of solution on a poly-L-lysine cover slip.

Fix grid or cover slip.

Rinse.

Osmicate grid.

Rinse and dry.

Sputter coat.

Thank you very much,

Jaci

Jaclynn Lett
Senior Research Technician
Research Center for Auditory and Visual Studies
Washington University School Of Medicine
Saint Louis, Missouri
lettj-at-ent.wustl.edu





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From: frank_karl-at-ardl.com
Date: Wed, 8 Feb 2012 14:27:36 -0600
Subject: [Microscopy] B+W printer response

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In my quest for an affordable printer to make B+W images of carbon black particles, I floated this question to the microscopy list server: "What's going to give me the nicest B+W TEM print?" After all, the images included in a report may be all the client knows about you.

I got a few interesting answers and in the process learned a bit about printers. But first, here's what I'm currently using: an HP2550 with copier paper. By the way the noise as stopped and the printer continues to run fine.

Here's what was suggested to me:
HP C5180,
HP2300,
HP8750,
Epson 800,
Sony Medical Printers.

Most people felt a color printer gave better gray scale images than a simple B+W printer but I was cautioned that a single black ink cartridge would be unable to create the entire tonal shading that good B+W prints need. Two different black ink cartridges could accomplish this.

Everyone had their own favorite printer and of course visiting vendor websites at 72 lines per inch of monitor resolution makes everyone's images look good. The best advice I got was to print images on as many printers as possible and then decide.

As a personal note, I'm not going to shop for printers right now, but I will try a ream of a better paper. Since several people print both text and images on our printer, running photo quality paper isn't an answer we can afford.

Increasing the resolution of our TEM camera would also be a big help,......


Thanks again for everyone's advice.

Frank






________________________________
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 8 Feb 2012 15:39:16 -0600
Subject: [Microscopy] viaWWW:Diamond knives & cleaning methods

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Email: slakmon-at-yahoo.com Name: Slakmon

Title-Subject: [Filtered] Diamond knives & cleaning methods

Message: Good Afternoon,

I was hoping to get people's feedback and opinion on the different
diamond knives on the market. Your opinion on new, resharpened would be
helpful as well. What methods that you found best suited in cleaning
your knives without damaging your knives. Please feel free to respond on
the group
or offline directly to my email address.

Thank you in advance,

Philip

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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 8 Feb 2012 16:58:48 -0600
Subject: [Microscopy] [Filtered] Re: viaWWW:Diamond knives cleaning methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

HI Philip-
I have tried knives from different sources but have always come back to
Diatome. New or resharpened, they are great. The HIsto knife is
wonderful for 0.5-2 um sections. I haven't used glass in years. The
ultras are great from end-to-end. You also get great customer support
from both the US and Swiss offices.
Lee Cohen-Gould, M.S., C.E.M.T.
Chair, MSA Certification Board

Sr. Staff Associate in Biochemistry and
Cell & Developmental Biology
Director, Electron Microscopy & Histology and Optical Microscopy Core
Facilities Weill Cornell Medical College

lcgould-at-med.cornell.edu
voice (212)746-6146
fax (212)746-8175
http://www.med.cornell.edu/research/rea_sup/
http://www.cornellbiochem.org
http://www.cornellcelldevbiology.org






On Feb 8, 2012, at 4:46 PM, microscopylistserver-noreply-at-microscopy.com
wrote:




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Email: slakmon-at-yahoo.com Name: Slakmon

Title-Subject: [Filtered] Diamond knives & cleaning methods

Message: Good Afternoon,

I was hoping to get people's feedback and opinion on the different
diamond knives on the market. Your opinion on new, resharpened would be
helpful as well. What methods that you found best suited in cleaning
your knives without damaging your knives. Please feel free to respond on
the group
or offline directly to my email address.

Thank you in advance,

Philip

Login Host: 184.163.214.160
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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 8 Feb 2012 16:58:57 -0600
Subject: [Microscopy] viaWWW:counting virus particles

Contents Retrieved from Microscopy Listserver Archives
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http://www.biotech.ufl.edu/EM/data/viruscount.html

Try the above link for help.

Cheers,

FC Monson

________________________________________
} From: microscopylistserver-noreply-at-microscopy.com [microscopylistserver-noreply-at-microscopy.com]
Sent: Friday, February 03, 2012 10:47 AM
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Email: mary.walker-at-csl.com.au Name: mary walker

Organization: csl limited

Title-Subject: [Filtered] counting virus particles

Message: Do any listers have experience with counting virus particles
with latex beads?
Is it possible to do better than order of magnitude?
What is the minimum number of particles per ml that can be counted?
What is the lower limit?

thanks in advance

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From: microscopylistserver-noreply-at-microscopy.com
Date: Wed, 8 Feb 2012 16:59:48 -0600
Subject: [Microscopy] [Filtered] Re: viaWWW:TEM : Focusing Problem

Contents Retrieved from Microscopy Listserver Archives
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Hello Mary,

I have done my fair share of virus particle counts over too many decades
and have some opinions about the process. Latex beads may be used in
counting but I would not recommend them as a standard except as a rough
size standard. The manufacturer of the beads would agree as well I
think. It is a supreme leap of faith to believe that beads adsorb and
remain adsorbed to formvar-carbon films or any other films in the same
manner as viruses. I also expect different viruses passively adsorb
differently as well. If you are using airfuge techniques the adsorption
issue may be somewhat improved but one still has to stain and assume
beads remain adsorbed to the grids exactly as the viruses. I know there
are many publications that have used latex beads as counting standards
but my understanding of viruses causes me to be skeptical of including
latex beads for counting. One can count the viruses directly using the
grid squares as a constant and then extrapolate, or even better with
digital images a digital image grid of precise size can be overlaid and
is helpful.

It is also risky to consider reliability beyond 10 -fold. My experience
is that there is considerable variability within the 10-fold limits. I
suspect this is true with most counting methods; EM or otherwise. The
major advantage with EM is that one can actually see aggregates when
they occur (another issue with virus counts if other counting methods
are used).

If one assumes on a 300 mesh grid with a grid hole of 61 microns, 20
particles within that grid square would represent approximately 107
particles per ml. Therefore, considering the fields one would have to
view and identify particles, then probably 106 particles per ml is about
the limit one would wish to search for counting. This is what I would
consider as a lower practical limit. One could improve on the lower
limit by looking at many-many grid squares but that would require
considerable searching, patience, and time.

I hope this is helpful; if you have additional questions I may be
reached by email or my address below.

Happy counting,

Charles Humphrey
Mailstop G32
US Centers for Disease Control and Prevention (CDC)
Atlanta, GA 30333




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} From: microscopylistserver-noreply-at-microscopy.com [mailto:microscopylistserver-noreply-at-microscopy.com]
Sent: Friday, February 03, 2012 10:49 AM
To: Humphrey, Charles (CDC/OID/NCEZID)

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walker

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Title-Subject: [Filtered] counting virus particles

Message: Do any listers have experience with counting virus particles
with latex beads?
Is it possible to do better than order of magnitude?
What is the minimum number of particles per ml that can be counted?
What is the lower limit?

thanks in advance

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Hi Ravi,

It could be that your sample was not at the 'standard focus' height. Set
your objective lens to the 'standard focus' value and then move your
sample height to get to focus.

I hope this helps.

Ke-Bin

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} Email: ravi.thakkar369-at-gmail.com Name: Ravindra Thakkar
}
} Title-Subject: [Filtered] TEM : Focusing Problem.
}
} Message: Image is not getting focused. The image is drastically moving
} while focusing, it moving highly even at low focus step & at low
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From: stefan.diller-at-t-online.de
Date: Thu, 9 Feb 2012 02:03:46 -0600
Subject: [Microscopy] Wanted: Visual Basic 6 programming skills

Contents Retrieved from Microscopy Listserver Archives
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Dear All,
I am looking for someone old enough to be fit in Visual Basic 6 ;-)
to help me with an existing VB6 program to remote my Philips 525 SEM.
Knowledge of German language would be perfect...
If interested, please contact me offline.

Best wishes,
Stefan
--


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Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.assisi.de
www.zwillingsprojekt.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 9 Feb 2012 02:21:22 -0600
Subject: [Microscopy] viaWWW:Diamond knives cleaning methods

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My experience with the Histo knife is similar. Its an incredible knife!

Dr. Gary B. Carr
Pacific Endodontic Research Foundation
San Diego, CA 92121
----- Original Message ----- } From:
{microscopylistserver-noreply-at-microscopy.com}
To: {gary-at-perfendo.com}
Sent: Wednesday, February 08, 2012 3:08 PM

I would request that you respond on-line. I would be interested in others'
views.

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood-at-partners.org

-----Original Message-----
} From: microscopylistserver-noreply-at-microscopy.com
[mailto:microscopylistserver-noreply-at-microscopy.com] Sent: Wednesday,
February 08, 2012 4:45 PM
To: Sherwood, Margaret

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Email: slakmon-at-yahoo.com Name: Slakmon

Title-Subject: [Filtered] Diamond knives & cleaning methods

Message: Good Afternoon,

I was hoping to get people's feedback and opinion on the different
diamond knives on the market. Your opinion on new, resharpened would be
helpful as well. What methods that you found best suited in cleaning
your knives without damaging your knives. Please feel free to respond on
the group
or offline directly to my email address.

Thank you in advance,

Philip

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From: microscopylistserver-noreply-at-microscopy.com
Date: Thu, 9 Feb 2012 02:39:38 -0600
Subject: [Microscopy] viaWWW:Carbon Coater for TEM and FESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'd have thought the osmium would be an unnecessary step since you're
coating the specimen before viewing. To fix the protein I'd be inclined
to use glutaraldehyde vapour - we've fixed problematic complexes before
by blotting the grid then holding it in the air space in a bottle of the
fixative to ten to thirty seconds. After a quick rinse you ought to be
able to dry it and put straight in the coater.
Cheers
Ian

http://www2.warwick.ac.uk/fac/sci/lifesci/facilities/imaging




-----Original Message-----
X-from: LettJ-at-ent.wustl.edu [mailto:LettJ-at-ent.wustl.edu]
Sent: 08 February 2012 19:54
To: Hands-Portman, Ian

Hello,

Our client wants us to image proteins using SEM. The protein is
dissolved in water and currently stored at -20degrees C. What do you
suggest? This is what I'm thinking:

Float a formvar-coated grid on drop of solution or place a drop of
solution on a poly-L-lysine cover slip.

Fix grid or cover slip.

Rinse.

Osmicate grid.

Rinse and dry.

Sputter coat.

Thank you very much,

Jaci

Jaclynn Lett
Senior Research Technician
Research Center for Auditory and Visual Studies Washington University
School Of Medicine Saint Louis, Missouri lettj-at-ent.wustl.edu





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Email: tomw-at-uidaho.edu Name: Tom Williams

Organization: University of Idaho

Title-Subject: [Filtered] Carbon Coater for TEM and FESEM
Message: Greetings.

I have decided that it is time to replace our "Classic" Varian
evaporative coater with a newer model. A day of replacing vacuum hoses,
and finding an inventory tag labeled: "Property of the Manhattan
Project," pushed me over the absorption edge.

Request: recommendations for a replacement unit? We operate a FESEM
and a 200kV TEM housed in a campus-wide Core Facility with a varied
client list (minerals, polymers, inorganics, and biologicals). We need
a coater suitable for routine SEM samples, EDS, hi-res FESEM work, TEM
grids, etc. so I'm looking for a relatively high-performance turbo
pumped system. I am prepared to expend a reasonable amount of capital
for this.

I would appreciate recommendations and/or suggestions for models,
vendors, etc.
Although I welcome contacts by vendors directly, I am looking for input
from end-users. Ranting diatribes are welcome as long as they are
entertaining!

Thanks,
Tom Williams




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