Microscopy ListServer Archives  


File Requested = MicroscopyArchive16.txt
Retrival Software Version=NJZ07060908

From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Jan 2016 12:58:49 -0600
Subject: [Microscopy] Administrivia - Happy New Year 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2015, the ListServer delivered 800+ messages to more than 4000 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated ~ 165+ Gbits of Email traffic and ~ 3.2 Million
Email messages were sent out this year by my tired and old little server.
Some time this year I will be migrating the Listserver to a new system, so
you might see a short outage.

As usual you don't want to know how much Junk Mail and spam has been filtered out.


The complete Microscopy ListServer Archives for 2015-1994 (~ are on-line at

http://www.microscopy.com.

A couple of IMPORTANT reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
your Email address from the listserver. The out-of-office / on-vacation
autoreply messages are a real nuisance to posters.

Do not reply to message with the return address of:

MicroscopyListserver-noreply-at-microscopy.com

these are messages forwarded usually from the WWW posting form. They do not
go back to the poster but rather into a black hole, which I rarely check.
If you see a message that has this "No-Reply" return address please post your
reply/comment/answer to:

Microscopy-at-microscopy.com

or if you wish to reply privately, look at the username in the body of
the message the originators Email address is usually listed therein.

As always if you have questions about suitability of postings or
are having problems, feel free to contact me at (zaluzec-at-microscopy.com)

Cheers,

Nestor
Your Friendly Neighborhood SysOp

--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================


==============================Original Headers==============================
21, 37 -- From microscopy.listserver-at-gmail.com Fri Jan 1 12:58:39 2016
21, 37 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180])
21, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u01Iwd1a004428
21, 37 -- for {microscopy-at-microscopy.com} ; Fri, 1 Jan 2016 12:58:39 -0600
21, 37 -- Received: by mail-ig0-f180.google.com with SMTP id to4so210644176igc.0
21, 37 -- for {microscopy-at-microscopy.com} ; Fri, 01 Jan 2016 10:58:30 -0800 (PST)
21, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
21, 37 -- d=gmail.com; s=20120113;
21, 37 -- h=from:reply-to:subject:to:message-id:date:user-agent:mime-version
21, 37 -- :content-type:content-transfer-encoding;
21, 37 -- bh=+a1jpd74Frg2Y5udiEzaw5OUFg41mSgJvuy41tXarUM=;
21, 37 -- b=OmAiXcLQbKbvokda2Sfyx0E24P5ChmJ4na4k8h28mR5WjtjjQ37rcwVeKmeGu6F1qX
21, 37 -- nIMN228E+xwLw07p1oOCV710eBsQ8nxVbSaAL8e/56GwPoyXiArtTXoo6ES3e3EDRhRG
21, 37 -- jq5mujVXJ4cFTG4+CaYjypsfhrwkqngwnWDz7mIcFfLOUQazdjPjWv7Lts9+65Twauzp
21, 37 -- C45nrSZ6AfS5WXadw0GJTCbjh33f4fan4kIZijvf7FpoHae16LE1FBAV1p3PoL/oBB3L
21, 37 -- kaASJaY0RWSi2Zt5Cnlgls6fy+0+pcR1ozRWlCJAV1AjfpazV18fUoqBgH26PZ4x1rp0
21, 37 -- xAuA==
21, 37 -- X-Received: by 10.50.137.40 with SMTP id qf8mr22997575igb.8.1451674710367;
21, 37 -- Fri, 01 Jan 2016 10:58:30 -0800 (PST)
21, 37 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
21, 37 -- by smtp.googlemail.com with ESMTPSA id g7sm17527511igc.15.2016.01.01.10.58.29
21, 37 -- for {microscopy-at-microscopy.com}
21, 37 -- (version=TLSv1/SSLv3 cipher=OTHER);
21, 37 -- Fri, 01 Jan 2016 10:58:29 -0800 (PST)
21, 37 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
21, 37 -- X-Google-Original-From: MicroscopyListserver-NoReply
21, 37 -- {microscopylistserver-noreply-at-microscopy.com}
21, 37 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
21, 37 -- Subject: Administrivia - Happy New Year 2016
21, 37 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
21, 37 -- Message-ID: {5686CC54.7020406-at-microscopy.com}
21, 37 -- Date: Fri, 1 Jan 2016 12:58:28 -0600
21, 37 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0)
21, 37 -- Gecko/20100101 Thunderbird/38.5.0
21, 37 -- MIME-Version: 1.0
21, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed
21, 37 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: Mon, 4 Jan 2016 13:36:41 -0600
Subject: [Microscopy] viaWWW:Job Opening at Bruker Nano Analytics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: don.becker-at-bruker.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both don.becker-at-bruker.com as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: don.becker-at-bruker.com
Name: Don Becker

Organization: Bruker Nano

Title-Subject: [Filtered] Job Opening at Bruker Nano Analytics

Message: Bruker Nano Analytics has an immediate opening for a Senior
Sales Representative for Microanalysis in the Southeastern United
States. The successful candidate will have a minimum of 3 -n 5 years
experience in the sale of scientific equipment, and will sell, market,
and support our microanalysis tools with new and existing customers, as
well as support our OEM sales channel partners. The sales territory
includes CO, NM, TX, OK, AL, NC, SC, FL, GA, LA, and MS.

A complete job description and application can be found at:

https://englishcareers-bruker.icims.com/jobs/3176/sr.-sales-representative---microanalysis/job?mobile=false&width=940&height=500&bga=true&needsRedirect=false&jan1offset=-300&jun1offset=-240


Login Host: 73.226.113.150
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
15, 40 -- From microscopy.listserver-at-gmail.com Mon Jan 4 13:36:41 2016
15, 40 -- Received: from mail-qk0-f179.google.com (mail-qk0-f179.google.com [209.85.220.179])
15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u04JafAP025097
15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 4 Jan 2016 13:36:41 -0600
15, 40 -- Received: by mail-qk0-f179.google.com with SMTP id p187so252255038qkd.1
15, 40 -- for {microscopy-at-microscopy.com} ; Mon, 04 Jan 2016 11:36:32 -0800 (PST)
15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 40 -- d=gmail.com; s=20120113;
15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 40 -- bh=2WucjmnV1esodSkd/8u+wO0RLVKe+o6Qo4tMTLP1pG4=;
15, 40 -- b=JEfexsLBSQ6u1FWfo2bATSDsxqrd3imZy0tM/dXGzh1I1j28ljKEuc6FVHs0kJKgrf
15, 40 -- uQlJuKxQT969tvRVXwCcwBfFMeJDWM7HgmDQcqHA/2NdD1nD7UGndwH4xP6gWQ+lu2Wy
15, 40 -- CojFbi+6wXAQ23TnDwSp/ZQ33pXhe9V5NI1PNo+DqluiCINQPu3EVDT+EQabDMJDrYWP
15, 40 -- NCZlBpQ8cHZa3K2Y/ESBQNbOE2EnpsD5x+J1uNr6YGxHNz6NxESslU0opBCj9vHzNHtC
15, 40 -- 9dYciCG1DozP3BB0dk+0ngqORq9GPf+0+faYZ22rYBoGfDuOjcfdsYf0dHMcZCEwEFam
15, 40 -- +1xQ==
15, 40 -- X-Received: by 10.55.71.135 with SMTP id u129mr119508399qka.26.1451936191941;
15, 40 -- Mon, 04 Jan 2016 11:36:31 -0800 (PST)
15, 40 -- Received: from aem120.amc.anl.gov ([146.139.72.120])
15, 40 -- by smtp.googlemail.com with ESMTPSA id a66sm31624900qkb.20.2016.01.04.11.36.31
15, 40 -- for {microscopy-at-microscopy.com}
15, 40 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 40 -- Mon, 04 Jan 2016 11:36:31 -0800 (PST)
15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply
15, 40 -- {microscopylistserver-noreply-at-microscopy.com}
15, 40 -- Subject: viaWWW:Job Opening at Bruker Nano Analytics
15, 40 -- References: {201601041748.u04Hmn4b022838-at-ns.microscopy.com}
15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
15, 40 -- X-Forwarded-Message-Id: {201601041748.u04Hmn4b022838-at-ns.microscopy.com}
15, 40 -- Message-ID: {568AC9B4.6050609-at-microscopy.com}
15, 40 -- Date: Mon, 4 Jan 2016 13:36:20 -0600
15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0)
15, 40 -- Gecko/20100101 Thunderbird/38.5.0
15, 40 -- MIME-Version: 1.0
15, 40 -- In-Reply-To: {201601041748.u04Hmn4b022838-at-ns.microscopy.com}
15, 40 -- Content-Type: text/plain; charset=UTF-8; format=flowed
15, 40 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================






From: wsalmon-at-wi.mit.edu
Date: Wed, 6 Jan 2016 15:42:09 -0600
Subject: [Microscopy] LM - 2016 Analytical and Quantitative Light Microscopy Course @ MBL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.

Laboratory exercises, demonstrations, and discussions include:
* geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
* phase contrast, polarization and interference microscopy;
* fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
* principles and application of digital imaging and quantitative digital image deconvolution;
* digital image processing and object identification and tracking;
* live cell and ratiometric imaging for FRET and ion concentration imaging;
* confocal microscopy and specialized methods such as TIRF and FLIM; and
* new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.

The course web site is at http://www.mbl.edu/aqlm .

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Applications due January 25 , 2016.

D irectors: Jagesh Shah (Harvard Medical School), Justin Taraska (National Institutes of Health)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Cheers,
Jagesh, Justin and Wendy

Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/

==============================Original Headers==============================
12, 39 -- From wsalmon-at-wi.mit.edu Wed Jan 6 15:42:09 2016
12, 39 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120])
12, 39 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u06Lg8aa011850
12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 15:42:08 -0600
12, 39 -- X-IronPort-AV: E=Sophos;i="5.20,530,1444708800";
12, 39 -- d="scan'208";a="3335172"
12, 39 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20])
12, 39 -- by ptolemy.wi.mit.edu with ESMTP; 06 Jan 2016 16:41:59 -0500
12, 39 -- Received: from localhost (localhost.localdomain [127.0.0.1])
12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 2518E31EB48
12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:59 -0500 (EST)
12, 39 -- Received: from mars.wi.mit.edu ([127.0.0.1])
12, 39 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032)
12, 39 -- with ESMTP id 88pSmo5FAWfT for {microscopy-at-microscopy.com} ;
12, 39 -- Wed, 6 Jan 2016 16:41:58 -0500 (EST)
12, 39 -- Received: from localhost (localhost.localdomain [127.0.0.1])
12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 9E23231EB62
12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:58 -0500 (EST)
12, 39 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu
12, 39 -- Received: from mars.wi.mit.edu ([127.0.0.1])
12, 39 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026)
12, 39 -- with ESMTP id vkLbfRjMwVqd for {microscopy-at-microscopy.com} ;
12, 39 -- Wed, 6 Jan 2016 16:41:58 -0500 (EST)
12, 39 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21])
12, 39 -- by mars.wi.mit.edu (Postfix) with ESMTP id 83F4331EAFE
12, 39 -- for {microscopy-at-microscopy.com} ; Wed, 6 Jan 2016 16:41:58 -0500 (EST)
12, 39 -- Date: Wed, 6 Jan 2016 16:41:58 -0500 (EST)
12, 39 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu}
12, 39 -- To: microscopy-at-microscopy.com
12, 39 -- Message-ID: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu}
12, 39 -- In-Reply-To: {1538907122.10462611.1452116467905.JavaMail.zimbra-at-wi.mit.edu}
12, 39 -- Subject: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL
12, 39 -- MIME-Version: 1.0
12, 39 -- Content-Type: text/plain; charset=utf-8
12, 39 -- Content-Transfer-Encoding: 7bit
12, 39 -- X-Originating-IP: [10.9.4.20]
12, 39 -- X-Mailer: Zimbra 8.0.7_GA_6021 (ZimbraWebClient - GC47 (Mac)/8.0.7_GA_6021)
12, 39 -- Thread-Topic: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL
12, 39 -- Thread-Index: 3I4RFXAc6NuHKgHiTFr8kP+ljEXmnA==
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Thu, 7 Jan 2016 08:45:06 -0600
Subject: [Microscopy] MegaViewII Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,
We have a trouble with our old MegaViewII TEM camera. Just before the
end of the last year, the power source of a PC we are using with
MegaViewII camera has gone. Just now we replaced the power source with
a new one. All seemed to be OK but when we tried to record the image
with MegaViewII camera we got a strange result. The image can be seen
on this link:

http://www2.biomed.cas.cz/~benada/MegaViewII_trouble.html


Please does anybody had to solve such problem?

Thanking for any response in advance.
Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic


Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.


==============================Original Headers==============================
10, 42 -- From benada-at-biomed.cas.cz Thu Jan 7 08:45:03 2016
10, 42 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11])
10, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u07Ej2X8016084
10, 42 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 08:45:03 -0600
10, 42 -- X-ASG-Debug-ID: 1452177892-05011e5425340270001-4CH8be
10, 42 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id cndEuIYa6CHL3S7G (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Thu, 07 Jan 2016 15:44:52 +0100 (CET)
10, 42 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz
10, 42 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32]
10, 42 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32
10, 42 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12])
10, 42 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
10, 42 -- (No client certificate requested)
10, 42 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 4D04B2C8070
10, 42 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 15:44:48 +0100 (CET)
10, 42 -- Date: Thu, 7 Jan 2016 15:44:47 +0100
10, 42 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
10, 42 -- To: {microscopy-at-microscopy.com}
10, 42 -- Subject: MegaViewII Trouble
10, 42 -- Message-ID: {20160107154447.26f75438-at-u117ob02}
10, 42 -- X-ASG-Orig-Subj: MegaViewII Trouble
10, 42 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV
10, 42 -- =?UTF-8?B?xIxS?=
10, 42 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu)
10, 42 -- MIME-Version: 1.0
10, 42 -- Content-Type: text/plain; charset=US-ASCII
10, 42 -- Content-Transfer-Encoding: 7bit
10, 42 -- X-IoP-CAS-MailScanner-ID: 4D04B2C8070.90340
10, 42 -- X-IoP-CAS-MailScanner: Processed
10, 42 -- X-Spam-Status: No
10, 42 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32]
10, 42 -- X-Barracuda-Start-Time: 1452177892
10, 42 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA
10, 42 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi
10, 42 -- X-Barracuda-Scan-Msg-Size: 1664
10, 42 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz
10, 42 -- X-Barracuda-BRTS-Status: 1
10, 42 -- X-Barracuda-Spam-Score: 0.00
10, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=
10, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25917
10, 42 -- Rule breakdown below
10, 42 -- pts rule name description
10, 42 -- ---- ---------------------- --------------------------------------------------
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 7 Jan 2016 14:55:18 -0600
Subject: [Microscopy] viaWWW: TEM Glove box sample transfer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: mlibbee-at-lbl.gov

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mlibbee-at-lbl.gov as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: mlibbee-at-lbl.gov
Name: Marissa

Organization: LBL

Title-Subject: [Filtered] Glove box

Message: To those of you who transfer samples to a TEM holder in a glove box, will you please
contact me offline with the name of the glove box supplier?

Thanks!

Login Host: 131.243.3.252
Listserver Email Form V - 20120416
---------------------------------------------------------------------------





--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
15, 40 -- From microscopy.listserver-at-gmail.com Thu Jan 7 14:55:16 2016
15, 40 -- Received: from mail-qg0-f48.google.com (mail-qg0-f48.google.com [209.85.192.48])
15, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u07KtEBH010432
15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 7 Jan 2016 14:55:15 -0600
15, 40 -- Received: by mail-qg0-f48.google.com with SMTP id e32so247793076qgf.3
15, 40 -- for {microscopy-at-microscopy.com} ; Thu, 07 Jan 2016 12:55:05 -0800 (PST)
15, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 40 -- d=gmail.com; s=20120113;
15, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
15, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 40 -- bh=wzdhJD5Y9ce3fflsfduzcRkZDzpQXc6DhbYciIWEdRk=;
15, 40 -- b=XigH4yoXVV5cMAHme/ZHgnkavbdBQwya05fLDWZkF17f4o27MF80LMPK4uf/9aPcci
15, 40 -- zWzKhPfTcWVpUciLjVyMH9TDRTkzkcxwZ1ROKtZ64kUz1Si9Fc6ZA4hiACcFKNvYjvvF
15, 40 -- 0swWFJi7jZ3Q5CUqurYB1DkVIamxlGos14AxNMq806c4CoLIULHxzpXavMXiACVcUm32
15, 40 -- crVMJJtGv/rFR6kADa3lwLFcjZvwSmOXuzcC567WTl+cO0RclC8hLrPGk3BEb/yYwOO6
15, 40 -- rVoTRp9sDO8cfUZf0hX8jqQotozwfhP6TPM8qulm/MKZUHPf3unGiKTCYq/c5YP/XETe
15, 40 -- H06g==
15, 40 -- X-Received: by 10.140.174.2 with SMTP id u2mr80610201qhu.62.1452200105375;
15, 40 -- Thu, 07 Jan 2016 12:55:05 -0800 (PST)
15, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
15, 40 -- by smtp.googlemail.com with ESMTPSA id y104sm18387621qgd.33.2016.01.07.12.55.04
15, 40 -- for {microscopy-at-microscopy.com}
15, 40 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 40 -- Thu, 07 Jan 2016 12:55:04 -0800 (PST)
15, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply
15, 40 -- {microscopylistserver-noreply-at-microscopy.com}
15, 40 -- Subject: viaWWW: TEM Glove box sample transfer
15, 40 -- References: {201601071953.u07JrlLx009792-at-ns.microscopy.com}
15, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
15, 40 -- X-Forwarded-Message-Id: {201601071953.u07JrlLx009792-at-ns.microscopy.com}
15, 40 -- Message-ID: {568ED0A7.4080400-at-microscopy.com}
15, 40 -- Date: Thu, 7 Jan 2016 14:55:03 -0600
15, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0)
15, 40 -- Gecko/20100101 Thunderbird/38.5.0
15, 40 -- MIME-Version: 1.0
15, 40 -- In-Reply-To: {201601071953.u07JrlLx009792-at-ns.microscopy.com}
15, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed
15, 40 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Fri, 8 Jan 2016 02:13:04 -0600
Subject: [Microscopy] Re: MegaViewII Trouble

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Warren, Ravi and Larry,
Thank you for your comments and suggestions. Yesterday I forgot one
important think, the pattern (image) that we are getting from
megaViewII does not depend on electron beam intensity. Even when the
beam is switched off, we get this pattern (image).

Best regards Oldrich

On Thu, 7 Jan 2016 15:58:40 +0000, "Straszheim, Warren E [BIOTC]"
{wesaia-at-iastate.edu} wrote :
} It looks like the synchronization has gone out. It is like what I see
} on our SEM when the raster on the sample doesn't match the raster on
} the screen.
}
} I presume the system is operating in TEM mode and that the MegaView
} is a CCD camera. If so, the only way that such a phenomenon might
} occur is if the number of pixels per line is not matching between the
} CCD and display. It seems the number of pixels across your image is
} slightly less than the number being read across the CCD. Therefore,
} each successive line is shifted some to the right.
}
} Is the sample a nice simple structure? I would wonder what a single
} vertical grid bar would look like.
}
} Warren
}
} -----Original Message-----
} From: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz]
} Sent: Thursday, January 07, 2016 8:48 AM
} To: Straszheim, Warren E [BIOTC]
} Subject: [Microscopy] MegaViewII Trouble
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello All,
} We have a trouble with our old MegaViewII TEM camera. Just before the
} end of the last year, the power source of a PC we are using with
} MegaViewII camera has gone. Just now we replaced the power source with
} a new one. All seemed to be OK but when we tried to record the image
} with MegaViewII camera we got a strange result. The image can be seen
} on this link:
}
} http://www2.biomed.cas.cz/~benada/MegaViewII_trouble.html
}
}
} Please does anybody had to solve such problem?
}
} Thanking for any response in advance.
} Oldrich
}
} --
} Oldrich Benada
} Institute of Microbiology CAS, v.v.i.
} Videnska 1083
} 142 20 Prague 4
} Czech Republic


Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.


==============================Original Headers==============================
7, 49 -- From benada-at-biomed.cas.cz Fri Jan 8 02:13:04 2016
7, 49 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11])
7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u088D3op013161
7, 49 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 02:13:04 -0600
7, 49 -- X-ASG-Debug-ID: 1452240773-05011e5425351cb0001-4CH8be
7, 49 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id oykzTSJlFOKTZZTf (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Fri, 08 Jan 2016 09:12:53 +0100 (CET)
7, 49 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz
7, 49 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32]
7, 49 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32
7, 49 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12])
7, 49 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
7, 49 -- (No client certificate requested)
7, 49 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 92E1E2C80FA;
7, 49 -- Fri, 8 Jan 2016 09:12:49 +0100 (CET)
7, 49 -- Date: Fri, 8 Jan 2016 09:12:49 +0100
7, 49 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
7, 49 -- To: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu} ,
7, 49 -- {microscopy-at-microscopy.com} , {les-at-zsgenetics.com} ,
7, 49 -- Ravi Thakkar
7, 49 -- {ravi.thakkar369-at-gmail.com}
7, 49 -- Subject: Re: [Microscopy] MegaViewII Trouble
7, 49 -- Message-ID: {20160108091249.40d565b0-at-u117ob02}
7, 49 -- X-ASG-Orig-Subj: Re: [Microscopy] MegaViewII Trouble
7, 49 -- In-Reply-To: {CY1PR04MB1813866E43A724B10C93B3B1D7F50-at-CY1PR04MB1813.namprd04.prod.outlook.com}
7, 49 -- References: {201601071447.u07Elg9u016882-at-ns.microscopy.com}
7, 49 -- {CY1PR04MB1813866E43A724B10C93B3B1D7F50-at-CY1PR04MB1813.namprd04.prod.outlook.com}
7, 49 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV
7, 49 -- =?UTF-8?B?xIxS?=
7, 49 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu)
7, 49 -- MIME-Version: 1.0
7, 49 -- Content-Type: text/plain; charset=US-ASCII
7, 49 -- Content-Transfer-Encoding: 7bit
7, 49 -- X-IoP-CAS-MailScanner-ID: 92E1E2C80FA.98AF0
7, 49 -- X-IoP-CAS-MailScanner: Processed
7, 49 -- X-Spam-Status: No
7, 49 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32]
7, 49 -- X-Barracuda-Start-Time: 1452240773
7, 49 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA
7, 49 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi
7, 49 -- X-Barracuda-Scan-Msg-Size: 3452
7, 49 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz
7, 49 -- X-Barracuda-BRTS-Status: 1
7, 49 -- X-Barracuda-Spam-Score: 0.00
7, 49 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_SC0_MISMATCH_TO
7, 49 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.25946
7, 49 -- Rule breakdown below
7, 49 -- pts rule name description
7, 49 -- ---- ---------------------- --------------------------------------------------
7, 49 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header
==============================End of - Headers==============================




From: mdelann1-at-jhmi.edu
Date: Fri, 8 Jan 2016 10:38:14 -0600
Subject: [Microscopy] Stabilizing pili on bacteria for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: gary.bloomer-at-montana.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both gary.bloomer-at-montana.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: gary.bloomer-at-montana.edu
Name: Gary Bloomer

Organization: Montana State University

Title-Subject: [Filtered] Chromophores characterized for 2-photon microscopy

Message: We are doing 2-photon microscopy but the available chromophores all seem to be
characterized only for 1 photon fluorescence. Setting parameters on the light source according to
this data may be creating an error in our results. Are others concerned with this and are there
solutions such as look-up tables available to properly set laser parameters for available dyes?

Login Host: 153.90.200.30
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
12, 40 -- From microscopy.listserver-at-gmail.com Fri Jan 8 07:44:15 2016
12, 40 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176])
12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08DiDcm012228
12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 07:44:14 -0600
12, 40 -- Received: by mail-io0-f176.google.com with SMTP id 77so255214254ioc.2
12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 08 Jan 2016 05:44:04 -0800 (PST)
12, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 40 -- d=gmail.com; s=20120113;
12, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
12, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 40 -- bh=m38CZ0tYTKgidhCo3hLR/qY8vTCi1OBYk0yJJ/0HiBM=;
12, 40 -- b=DztjP7xQD90BIdzGMR5c3w2b2nq61+L2m9OHkC2SnoX9RtmgHQtW0QjunDQhR2E6X/
12, 40 -- nSCEPDH1lcce7uA2sgDt1XrFHCpKSCh5q89dluFOGf3D1l0GAxstfPjtMRSraGBn1xik
12, 40 -- WooLx9ou6aEIIFAzH9gq2VtV77ZzRxbP5pq9FJ6Zi9VDiL6oTq8BsW3HNghOq8Bcbtja
12, 40 -- au1EvO6ra0j35HTwo56DXdGcesCOr1PGmEgCpjVQM/6fSnOAc4zIdLVbuPUPJtOLcKEo
12, 40 -- vSjz9n1uAtttqHUZCJ6Nuev52eOhKfpSf/kuI76RDZGM8DcW6nTShh+ixBFWs2DrIryJ
12, 40 -- HwYQ==
12, 40 -- X-Received: by 10.107.9.224 with SMTP id 93mr97073491ioj.112.1452260644386;
12, 40 -- Fri, 08 Jan 2016 05:44:04 -0800 (PST)
12, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
12, 40 -- by smtp.googlemail.com with ESMTPSA id n6sm6735362ige.12.2016.01.08.05.44.03
12, 40 -- for {microscopy-at-microscopy.com}
12, 40 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 40 -- Fri, 08 Jan 2016 05:44:03 -0800 (PST)
12, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply
12, 40 -- {microscopylistserver-noreply-at-microscopy.com}
12, 40 -- Subject: viaWWW:Chromophores characterized for 2-photon microscopy
12, 40 -- References: {201601072301.u07N1pve032564-at-ns.microscopy.com}
12, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
12, 40 -- X-Forwarded-Message-Id: {201601072301.u07N1pve032564-at-ns.microscopy.com}
12, 40 -- Message-ID: {568FBD23.1090507-at-microscopy.com}
12, 40 -- Date: Fri, 8 Jan 2016 07:44:03 -0600
12, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0)
12, 40 -- Gecko/20100101 Thunderbird/38.5.0
12, 40 -- MIME-Version: 1.0
12, 40 -- In-Reply-To: {201601072301.u07N1pve032564-at-ns.microscopy.com}
12, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed
12, 40 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From hanja07656515151viu-at-gmail.com Fri Jan 8 08:09:14 2016
Return-Path: {hanja07656515151viu-at-gmail.com}
Received: from gmail.com (220-134-188-200.HINET-IP.hinet.net [220.134.188.200])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u08E9BX8030857
for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 8 Jan 2016 08:09:13 -0600
Message-ID: {C2B18D2B.24421114-at-gmail.com}

Dear Listers,

I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them through a small Millipore filter and then processing this filter disc in the usual way up through CPD, mounting, sputter coating with gold/palladium for SEM. Most of the pili seem to have fallen off the bacteria, plenty of loose pili on the filter beside the bacteria.

Couple of questions for everybody. First, can fixation cause pili to shear off the bacteria? Second, anyone have any suggestions on how to keep the pili attached to the bacteria for SEM observation? Thanks in advance, all help is appreciated.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



==============================Original Headers==============================
8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016
8, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126])
8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08EQuO4003928
8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:26:56 -0600
8, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1])
8, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id B5B455C3BF6
8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST)
8, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1])
8, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
8, 37 -- (No client certificate requested)
8, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 070435C3B2D
8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20 -0600 (CST)
8, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by
8, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id
8, 37 -- 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600
8, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu}
8, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
8, 37 -- Subject: Stabilizing pili on bacteria for SEM
8, 37 -- Thread-Topic: Stabilizing pili on bacteria for SEM
8, 37 -- Thread-Index: AdFKH6WOLCX89yPzR5e9+rDjaEY6nA==
8, 37 -- Date: Fri, 8 Jan 2016 14:26:44 +0000
8, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org}
8, 37 -- Accept-Language: en-US
8, 37 -- Content-Language: en-US
8, 37 -- X-MS-Has-Attach:
8, 37 -- X-MS-TNEF-Correlator:
8, 37 -- x-originating-ip: [10.8.64.15]
8, 37 -- Content-Type: text/plain; charset="us-ascii"
8, 37 -- MIME-Version: 1.0
8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce
8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu
8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d
8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext
8, 37 -- X-VPM-CERT-FLAG: 0
8, 37 -- X-VPM-IS-HYBRID: 0
8, 37 -- Content-Transfer-Encoding: 8bit
8, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08EQuO4003928
==============================End of - Headers==============================

From advertise.bz222uuv-at-gmail.com Fri Jan 8 09:32:23 2016
Return-Path: {advertise.bz222uuv-at-gmail.com}
Received: from gmail.com ([1.236.84.191])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u08FWKsx027065
for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 8 Jan 2016 09:32:21 -0600
Message-ID: {62EBFA9A.D09DB7CA-at-gmail.com}

Tom,
Are you following the primary fixative with osmium? Does this strain have
pili? You can run a quick negative stain prep to confirm pili.
Also maybe some tannic acid in the primary fixative along with PF and Ga. I
never heard of pili falling of during fixation.

Good luck,
Michael Delannoy

-----Original Message-----
X-from: tbargar-at-unmc.edu [mailto:tbargar-at-unmc.edu]
Sent: Friday, January 08, 2016 9:38 AM
To: delannoy-at-jhmi.edu

Dear Listers,

I'm currently fixing a strain of E. Coli with pili with 2%GA , 2%Paraform.
In .1M Sorensen's phosphate buffer. Filtering them through a small
Millipore filter and then processing this filter disc in the usual way up
through CPD, mounting, sputter coating with gold/palladium for SEM. Most of
the pili seem to have fallen off the bacteria, plenty of loose pili on the
filter beside the bacteria.

Couple of questions for everybody. First, can fixation cause pili to shear
off the bacteria? Second, anyone have any suggestions on how to keep the
pili attached to the bacteria for SEM observation? Thanks in advance, all
help is appreciated.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended
only for the use of the addressee(s) above. Any unauthorized use or
disclosure of this information is prohibited. If you have received this
e-mail by mistake, please delete it and immediately contact the sender.



==============================Original Headers==============================
8, 37 -- From tbargar-at-unmc.edu Fri Jan 8 08:26:56 2016 8, 37 -- Received:
from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126])
8, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u08EQuO4003928
8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:26:56
-0600
8, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1])
8, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id B5B455C3BF6
8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20
-0600 (CST)
8, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1])
8, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
8, 37 -- (No client certificate requested)
8, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id
070435C3B2D
8, 37 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 08:07:20
-0600 (CST)
8, 37 -- Received: from UNMCEX2.unmcresforest.org
([fe80::151d:89d:49b6:a0ab]) by 8, 37 -- UNMCEX1.unmcresforest.org
([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 -- 14.03.0235.001; Fri,
8 Jan 2016 08:26:45 -0600 8, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu}
8, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 37
-- Subject: Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Topic:
Stabilizing pili on bacteria for SEM 8, 37 -- Thread-Index:
AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8 Jan 2016 14:26:44
+0000 8, 37 -- Message-ID:
{E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org}
8, 37 -- Accept-Language: en-US
8, 37 -- Content-Language: en-US
8, 37 -- X-MS-Has-Attach:
8, 37 -- X-MS-TNEF-Correlator:
8, 37 -- x-originating-ip: [10.8.64.15]
8, 37 -- Content-Type: text/plain; charset="us-ascii"
8, 37 -- MIME-Version: 1.0
8, 37 -- X-VPM-MSG-ID: 86ea5b32-28f1-4bca-b83d-6df6163209ce
8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu
8, 37 -- X-VPM-GROUP-ID: f91a6e30-52f8-4523-846a-55fd5677fb4d
8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext 8, 37 -- X-VPM-CERT-FLAG: 0 8,
37 -- X-VPM-IS-HYBRID: 0 8, 37 -- Content-Transfer-Encoding: 8bit 8, 37 --
X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id
u08EQuO4003928 ==============================End of -
Headers==============================


==============================Original Headers==============================
16, 24 -- From prvs=808477f31=mdelann1-at-jhmi.edu Fri Jan 8 10:38:14 2016
16, 24 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.130])
16, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08GcE5W027977
16, 24 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 10:38:14 -0600
16, 24 -- X-IronPort-AV: E=Sophos;i="5.20,539,1444708800";
16, 24 -- d="scan'208";a="204135271"
16, 24 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96])
16, 24 -- by IPEB1.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 08 Jan 2016 11:38:03 -0500
16, 24 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu}
16, 24 -- To: {tbargar-at-unmc.edu}
16, 24 -- Cc: {Microscopy-at-microscopy.com} , {microscopy.listserver-at-gmail.com} ,
16, 24 -- {microscopy-at-msa.microscopy.com}
16, 24 -- References: {201601081437.u08EbZvM010463-at-ns.microscopy.com}
16, 24 -- In-Reply-To: {201601081437.u08EbZvM010463-at-ns.microscopy.com}
16, 24 -- Subject: RE: [Microscopy] Stabilizing pili on bacteria for SEM
16, 24 -- Date: Fri, 8 Jan 2016 11:38:06 -0500
16, 24 -- Message-ID: {007b01d14a32$f3daad70$db900850$-at-jhmi.edu}
16, 24 -- MIME-Version: 1.0
16, 24 -- Content-Type: text/plain;
16, 24 -- charset="us-ascii"
16, 24 -- Content-Transfer-Encoding: 7bit
16, 24 -- X-Mailer: Microsoft Outlook 14.0
16, 24 -- Thread-Index: AQKcnZohv6HMCmg8szDXNpP2BC84M51bKVGg
16, 24 -- Content-Language: en-us
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Fri, 8 Jan 2016 11:05:37 -0600
Subject: [Microscopy] Re: Stabilizing pili on bacteria for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Tom,
We are using E. coli in our practical courses of electron microscopy in
biology. We usually process E. coli samples for TEM and SEM in
parallel.

At first, do you really need a SEM for imaging E. coli pili?
TEM and negative staining is much more faster a you even do not need to
fix E. coli sample at all. In our hand 1% ammonium molybdate, pH6.8-7.0
plus 0.1% trehalose mixture works quite well for negative staining of
bacteria on glow-discharge treated formvar/carbon grids.

Our procedure for SEM:
1. Start with 0.5 ml of E. coli culture; optical density ranging from
0.4 to 0.6.
2. Fix it with 3% glutaraldehyde for half an hour at room
temperature, then continue the fixation at 4 oC overnight.
3. Wash the fixed cells well with phosphate or cacodylate buffer
(three times at least).
4. Make a moist chamber from Petri dish, wet filter paper and Parafilm.
5. Place some poly-l-lysine treated circular cover-slips on the
Parafilm in the moist chamber and put 100 ,Aei 200 uL of fixed cells in the
washing buffer onto the individual cover-slip. Whole cover-slip area
should be covered with liquid. Let sediment the cells onto the
cover-slips at 4 oC overnight.
6. Wash the cover-slips with ddH2O.
7. Dehydrate through alcohol series; CPD; ... .

With this procedure the pili should stay attached to bacteria.

Best regards
Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic


On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu wrote :
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} I'm currently fixing a strain of E. Coli with pili with 2%GA ,
} 2%Paraform. In .1M Sorensen's phosphate buffer. Filtering them
} through a small Millipore filter and then processing this filter disc
} in the usual way up through CPD, mounting, sputter coating with
} gold/palladium for SEM. Most of the pili seem to have fallen off the
} bacteria, plenty of loose pili on the filter beside the bacteria.
}
} Couple of questions for everybody. First, can fixation cause pili to
} shear off the bacteria? Second, anyone have any suggestions on how
} to keep the pili attached to the bacteria for SEM observation?
} Thanks in advance, all help is appreciated.
}
} Tom Bargar
} UNMC
} Electron Microscopy Core Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
}
} The information in this e-mail may be privileged and confidential,
} intended only for the use of the addressee(s) above. Any unauthorized
} use or disclosure of this information is prohibited. If you have
} received this e-mail by mistake, please delete it and immediately
} contact the sender.
}
}
}
} ==============================Original
} Headers============================== 8, 37 -- From tbargar-at-unmc.edu
} Fri Jan 8 08:26:56 2016 8, 37 -- Received: from zixvpm01.unmc.edu
} (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 -- by
} ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} u08EQuO4003928 8, 37 -- for {Microscopy-at-microscopy.com} ; Fri,
} 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from 127.0.0.1 (ZixVPM
} [127.0.0.1]) 8, 37 -- by Outbound.unmc.edu (Proprietary) with



From: microwink-at-gmail.com
Date: Fri, 8 Jan 2016 12:22:17 -0600
Subject: [Microscopy] Job Opening: TEM Applications Specialist at Virginia Tech in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Virginia Tech in Blacksburg, VA, USA has an opening for an
Applications Specialist in Scanning and Transmission Electron
Microscopy. This position will help support internal and external
users of the Virginia Tech National Center for Earth and Environmental
Nanotechnology Infrastructure (NCE- {=NI) under the direct supervision of
NCE- {=NI Associate Director for Instrumentation & Technical Development.
The successful candidate will have their office located within the
Nanoscale Characterization and Fabrication Laboratory (NCFL). The NCFL
houses advanced field emission scanning electron microscopes with EDS
and EBSD capabilities, several transmission electron microscopes with
EDS, tomography, HRTEM, cryoTEM, and other capabilities, and a suite
of powerful surface spectroscopy tools including magnetic sector SIMS,
XPS, and more. Multiple sample preparation laboratories facilitate the
preparation of samples for analysis using SEM, TEM, AFM, EBSD, and
other techniques.

The general duties associated with the TEM Applications Specialist position are:

(a) TEM instrument operation including imaging, diffraction, and spectroscopy.
(b) TEM data analysis support including image analysis, EDS
quantification, diffraction pattern indexing, and more.
(c) Conduct directed pilot projects and help co-author scientific publications.
(d) Sample preparation and management for both internal and external users.

Qualified candidates should have experience with electron microscopy,
x-ray spectroscopy, and electron diffraction techniques including nano
beam diffraction and convergent beam electron diffraction. Candidates
should be comfortable assisting users in the safe and effective
operation of the S/TEM instruments. Candidates should be able to
effectively communicate with faculty, staff and students from a
variety of technical and cultural backgrounds.

Scientist/engineers experienced in Earth systems science are
especially encouraged to apply. Note: the majority of samples are
expected to be poorly crystallized, polymorphous, naturally occurring
materials ranging from micro- to nanoscale.

To view a more detailed list of expected duties and the qualifications
required, visit:

https://listings.jobs.vt.edu/postings/62637

Applicants can apply to this position by visiting the above link and
clicking on the "Apply for this Job" link near the top of the page. If
the above link does not work, then you can apply by visiting
www.jobs.vt.edu and searching for posting number SR0150183.

Virginia Tech does not discriminate against employees, students, or
applicants on the basis of age, color, disability, gender, gender
identity, gender expression, national origin, political affiliation,
race, religion, sexual orientation, genetic information, veteran
status, or any other basis protected by law. For inquiries regarding
non-discrimination policies, contact the executive director for Equity
and Access at 540-231-8771 or Virginia Tech, North End Center, Suite
2300 (0318), 300 Turner St. NW, Blacksburg, VA 24061.


==============================Original Headers==============================
10, 30 -- From microwink-at-gmail.com Fri Jan 8 12:22:17 2016
10, 30 -- Received: from mail-ig0-f193.google.com (mail-ig0-f193.google.com [209.85.213.193])
10, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u08IMHZh005236
10, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 12:22:17 -0600
10, 30 -- Received: by mail-ig0-f193.google.com with SMTP id ik10so8713867igb.1
10, 30 -- for {Microscopy-at-microscopy.com} ; Fri, 08 Jan 2016 10:22:08 -0800 (PST)
10, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 30 -- d=gmail.com; s=20120113;
10, 30 -- h=mime-version:date:message-id:subject:from:to:content-type
10, 30 -- :content-transfer-encoding;
10, 30 -- bh=Qy3rsPDs60GZtmT+nsLZLvLQbXfPQTkDeqGcyEFyfv4=;
10, 30 -- b=oKmeBNbLnpu2JZytDPFtB/GKVBJYMRXXbHxjOjSDQM+7CIT818gvDtkrK7KBGOaaxf
10, 30 -- GSCQxsoDPa2QYxSYPRfYwPax0rFsQgwLetfyat6EQbDmapH4wLtV7cZVPYa0MsivojEv
10, 30 -- SLTCH1EKn+tz3qTKf3V0FJWD2u69r2yGNLXXrHQOT+q/pTjCjDbmCPf1TVqMa3f9EOJk
10, 30 -- B2BFKQuiWjCMT0i9gqK9Duy0zn7EiNKY9ok9ITVonvbcFvBEPZtUZodAFlfWMelDL6WL
10, 30 -- hPvy94wT4Mfb1lqtB2fEhed39Tha1u7rJwP4hsBxJwFI9NPO/11zUnsBqj8wJTzrtPm9
10, 30 -- Wdhw==
10, 30 -- MIME-Version: 1.0
10, 30 -- X-Received: by 10.50.61.132 with SMTP id p4mr302308igr.55.1452277328119; Fri,
10, 30 -- 08 Jan 2016 10:22:08 -0800 (PST)
10, 30 -- Received: by 10.107.144.136 with HTTP; Fri, 8 Jan 2016 10:22:08 -0800 (PST)
10, 30 -- Date: Fri, 8 Jan 2016 13:22:08 -0500
10, 30 -- Message-ID: {CAA8T2PO3DEKtf++jkgz-vTo0phOL8DW-PP9oWMSpEwr89YALqA-at-mail.gmail.com}
10, 30 -- Subject: Job Opening: TEM Applications Specialist at Virginia Tech in
10, 30 -- Blacksburg, VA, USA
10, 30 -- From: Christopher Winkler {microwink-at-gmail.com}
10, 30 -- To: Microscopy-at-microscopy.com
10, 30 -- Content-Type: text/plain; charset=UTF-8
10, 30 -- Content-Transfer-Encoding: 8bit
10, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u08IMHZh005236
==============================End of - Headers==============================




From: dave-at-boeckeler.com
Date: Mon, 11 Jan 2016 13:55:41 -0600
Subject: [Microscopy] job opening at RMC-Boeckeler in Tucson, Arizona

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jtwilley-at-sprynet.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jtwilley-at-sprynet.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: jtwilley-at-sprynet.com
Name: John Twilley

Organization: Conservation Scientist

Title-Subject: [Filtered] Craic Microspectrophotometer Users

Message: Is there anyone on the listserve in the NYC area with a Craic unit who would be willing to
assist with measurement of the reflectance curve from a paint fragment?

Login Host: 24.44.53.206
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
12, 40 -- From microscopy.listserver-at-gmail.com Fri Jan 8 18:32:55 2016
12, 40 -- Received: from mail-ig0-f181.google.com (mail-ig0-f181.google.com [209.85.213.181])
12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u090WtcO000819
12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jan 2016 18:32:55 -0600
12, 40 -- Received: by mail-ig0-f181.google.com with SMTP id mw1so83778216igb.1
12, 40 -- for {microscopy-at-microscopy.com} ; Fri, 08 Jan 2016 16:32:46 -0800 (PST)
12, 40 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 40 -- d=gmail.com; s=20120113;
12, 40 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
12, 40 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 40 -- bh=ya3vRlAlyaerNemqk5p871jv09fzsquWk8DgRFAsoRI=;
12, 40 -- b=kuKAf2YZ1Ujpn5FfsxIoSCzm5nov4vwMN19ZKRnxQQhN/THO2AohiJWk83TzX4t+6U
12, 40 -- kJG6j+b6tmdbf436FMZSn/qZJRdfa09gbLUke17lK39ptnrZu4gkmMXf7MIgUUbL8CxY
12, 40 -- jNfiexkpkAgqIHv2Htvh/24KMTeCPxtQLeftFNPL+RFKV2ARh/oxI1GffPVcCbrpPfIF
12, 40 -- ujewB82aycWyelLlXG7fCnP1HNwFCEG1VKePkcyhs4ol1CXjrMktzi5fl0K6eO2vCWMQ
12, 40 -- 1XTJSuiT9PQyCmLXx0oTejwimWqEVW5EXZVxaeimlqbwyxZ8vmtliI33pUWPpP8tKeJl
12, 40 -- kb3g==
12, 40 -- X-Received: by 10.50.115.106 with SMTP id jn10mr1718803igb.77.1452299565942;
12, 40 -- Fri, 08 Jan 2016 16:32:45 -0800 (PST)
12, 40 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
12, 40 -- by smtp.googlemail.com with ESMTPSA id 84sm36193616ioh.3.2016.01.08.16.32.45
12, 40 -- for {microscopy-at-microscopy.com}
12, 40 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 40 -- Fri, 08 Jan 2016 16:32:45 -0800 (PST)
12, 40 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 40 -- X-Google-Original-From: MicroscopyListserver-NoReply
12, 40 -- {microscopylistserver-noreply-at-microscopy.com}
12, 40 -- Subject: viaWWW:Craic Microspectrophotometer Users
12, 40 -- References: {201601082018.u08KIuDF027218-at-ns.microscopy.com}
12, 40 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 40 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
12, 40 -- X-Forwarded-Message-Id: {201601082018.u08KIuDF027218-at-ns.microscopy.com}
12, 40 -- Message-ID: {5690552C.2090203-at-microscopy.com}
12, 40 -- Date: Fri, 8 Jan 2016 18:32:44 -0600
12, 40 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.9; rv:38.0)
12, 40 -- Gecko/20100101 Thunderbird/38.5.1
12, 40 -- MIME-Version: 1.0
12, 40 -- In-Reply-To: {201601082018.u08KIuDF027218-at-ns.microscopy.com}
12, 40 -- Content-Type: text/plain; charset=windows-1252; format=flowed
12, 40 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From improve.alexa.rankslsuzn-at-gmail.com Sat Jan 9 13:10:08 2016
Return-Path: {improve.alexa.rankslsuzn-at-gmail.com}
Received: from gmail.com ([211.63.33.142])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u09JA2g5002263
for {microscopylistserverarchive5-at-microscopy.com} ; Sat, 9 Jan 2016 13:10:05 -0600
Message-ID: {F8A49867.6949C4DE-at-gmail.com}

Dear Microscopists,

We have an entry level opening in our Tucson
manufacturing facility suitable for an enthusiastic
energetic
recent graduate with good communication skills, to
join us as a full time EM tech in our applications
support lab.

Initial duties include:-

* Ultramicrotome QC - cut ultra thin sections &
evaluate in TEM + basic QC evaluation of sub
assemblies/components + submit reports &
documentation
* Glass Knife Maker test, calibration and QC reports
* Rotary microtome QC - cut semi-thin to thick
paraffin and materials science samples -
evaluate & report
* TEM maintenance

with opportunities for growth in the following areas:

* Applications support evaluating customer specimens
and advising on appropriate sample prep
techniques - includes
both life science and materials science disciplines
* Cryo Ultramicrotome test and evaluation
* Assist with workshops and product demonstrations
* Field support if applicant is willing and able to
travel
* Sales and Service

Please respond by email to info-at-boeckeler.com

--
Dave Roberts
Director-RMC Microscopy Products
RMC-Boeckeler
Boeckeler Instruments Inc
4650 S Butterfield Drive
Tucson, Arizona 85714
Tel: 520-745-0001
Fax: 520-745-0004
www.rmcproducts.com
Skype:dave.robertsrmc


==============================Original Headers==============================
9, 24 -- From dave-at-boeckeler.com Mon Jan 11 13:55:41 2016
9, 24 -- Received: from smtp69.ord1c.emailsrvr.com (smtp69.ord1c.emailsrvr.com [108.166.43.69])
9, 24 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0BJtffI008900
9, 24 -- for {microscopy-at-microscopy.com} ; Mon, 11 Jan 2016 13:55:41 -0600
9, 24 -- Received: from smtp1.relay.ord1c.emailsrvr.com (localhost.localdomain [127.0.0.1])
9, 24 -- by smtp1.relay.ord1c.emailsrvr.com (SMTP Server) with ESMTP id 4001B3805C2;
9, 24 -- Mon, 11 Jan 2016 14:55:31 -0500 (EST)
9, 24 -- X-Auth-ID: dave-at-boeckeler.com
9, 24 -- Received: by smtp1.relay.ord1c.emailsrvr.com (Authenticated sender: dave-AT-boeckeler.com) with ESMTPSA id B4D013805D2;
9, 24 -- Mon, 11 Jan 2016 14:55:30 -0500 (EST)
9, 24 -- X-Sender-Id: dave-at-boeckeler.com
9, 24 -- Received: from [10.0.1.241] (wsip-68-98-221-238.ph.ph.cox.net [68.98.221.238])
9, 24 -- (using TLSv1.2 with cipher DHE-RSA-AES128-SHA)
9, 24 -- by 0.0.0.0:465 (trex/5.5.4);
9, 24 -- Mon, 11 Jan 2016 14:55:31 -0500
9, 24 -- Message-ID: {569408AC.4030306-at-boeckeler.com}
9, 24 -- Date: Mon, 11 Jan 2016 12:55:24 -0700
9, 24 -- From: Dave Roberts {dave-at-boeckeler.com}
9, 24 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:31.0) Gecko/20100101 Thunderbird/31.4.0
9, 24 -- MIME-Version: 1.0
9, 24 -- To: microscopy-at-microscopy.com
9, 24 -- Subject: job opening at RMC-Boeckeler in Tucson, Arizona
9, 24 -- Content-Type: text/plain; charset=utf-8; format=flowed
9, 24 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Wed, 13 Jan 2016 12:31:52 -0600
Subject: [Microscopy] iPhone microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My apologies for cross-posting...

Someone has asked me about microscope attachments for iPhone. I've seen
lots of ads, but I haven't looked closely at any of the products. Do any
of you have opinions about products that turn your iPhone or Android into
decent microscopes? I'm not sure if she wants to go with a stand and
slides, or just take nicely magnified images on the go. I think the
former...

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016
4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DIVppj017621
4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 -0600
4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1])
4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0DIVVV9025775
4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST)
4, 21 -- Received: from localhost (tina-at-localhost)
4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0DIVUQ2025771;
4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST)
4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs
4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST)
4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
4, 21 -- X-X-Sender: tina-at-b1000
4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU
4, 21 -- Subject: iPhone microscope
4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000}
4, 21 -- MIME-Version: 1.0
4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================




From: steven.spurgeon-at-pnnl.gov
Date: Wednesday, January 13, 2016 at 10:56 AM
Subject: [Microscopy] iPhone microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Tina,

Here at PNNL we,Aeove actually developed a low-cost (~$1) cellphone camera that magnifies up to 1000x, depending on the specification. Our lab has freely released the blueprints for 3D printing or you can order pre-manufactured lenses that clip onto pretty much any smartphone. We use them frequently in our demos to high school and middle school children and they work quite well once you get the hang of them.

Here,Aeos a link to more details: http://availabletechnologies.pnnl.gov/technology.asp?id=393

Feel free to contact me if you have any questions.

* The views and opinions expressed in this email are my own and do not necessarily reflect those of PNNL, the Battelle Memorial Institute, the United States Government, or any agency thereof.
______________________________________
Steven R. Spurgeon, Ph.D.
Postdoctoral Research Associate
Physical and Computational Sciences Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:K8-87
Richland, WA 99352

Tel: +1-509-371-7123
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}




X-from: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
Reply-To: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}

My apologies for cross-posting...

Someone has asked me about microscope attachments for iPhone. I've seen
lots of ads, but I haven't looked closely at any of the products. Do any
of you have opinions about products that turn your iPhone or Android into
decent microscopes? I'm not sure if she wants to go with a stand and
slides, or just take nicely magnified images on the go. I think the
former...

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016
4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30])
4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DIVppj017621
4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52 -0600
4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1])
4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0DIVVV9025775
4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST)
4, 21 -- Received: from localhost (tina-at-localhost)
4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0DIVUQ2025771;
4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST)
4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs
4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST)
4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
4, 21 -- X-X-Sender: tina-at-b1000
4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU
4, 21 -- Subject: iPhone microscope
4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000}
4, 21 -- MIME-Version: 1.0
4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================



==============================Original Headers==============================
22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13 13:11:57 2016
22, 29 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov [192.101.109.63])
22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DJBuxL006200
22, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 13:11:56 -0600
22, 29 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212])
22, 29 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 13 Jan 2016 11:11:45 -0800
22, 29 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.82]) by EX10CASHUB06.pnnl.gov
22, 29 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Wed, 13 Jan 2016 11:11:45
22, 29 -- -0800
22, 29 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov}
22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} ,
22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
22, 29 -- Subject: Re: [Microscopy] iPhone microscope
22, 29 -- Thread-Topic: [Microscopy] iPhone microscope
22, 29 -- Thread-Index: AQHRTjQyIKPRV14ru0iCfvZUlj7Hu5750BIA
22, 29 -- Date: Wed, 13 Jan 2016 19:11:44 +0000
22, 29 -- Message-ID: {062CD028-BFB5-47EE-A028-A5F9044AB27E-at-pnnl.gov}
22, 29 -- References: {201601131856.u0DIudAw004154-at-ns.microscopy.com}
22, 29 -- In-Reply-To: {201601131856.u0DIudAw004154-at-ns.microscopy.com}
22, 29 -- Accept-Language: en-US
22, 29 -- Content-Language: en-US
22, 29 -- X-MS-Has-Attach:
22, 29 -- X-MS-TNEF-Correlator:
22, 29 -- x-originating-ip: [130.20.128.10]
22, 29 -- Content-Type: text/plain; charset="utf-8"
22, 29 -- Content-ID: {D94556B5D077EF4595F2908D97115031-at-pnnl.gov}
22, 29 -- MIME-Version: 1.0
22, 29 -- Content-Transfer-Encoding: 8bit
22, 29 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u0DJBuxL006200
==============================End of - Headers==============================




From: FB.Fans.Cheap-at-cdn.rongdexuan.cn
Date: 14 Jan 2016 07:16:19 +0200
Subject: re: Stable Fanpage FB Likes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Tina,
Though not as cheap as the PNNL design, some colleagues of mine have
used this inexpensive, hardware-store-materials-based home-built design
for outreach and liked it:
http://www.instructables.com/id/10-Smartphone-to-digital-microscope-conversion/
Tyler Harvey
University of Oregon

On 2016-01-13 11:29, steven.spurgeon-at-pnnl.gov wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello Tina,
}
} Here at PNNL we/c,C ,Ncve actually developed a low-cost (~$1) cellphone
} camera that magnifies up to 1000x, depending on the specification. Our
} lab has freely released the blueprints for 3D printing or you can
} order pre-manufactured lenses that clip onto pretty much any
} smartphone. We use them frequently in our demos to high school and
} middle school children and they work quite well once you get the hang
} of them.
}
} Here/c,C ,Ncs a link to more details:
} http://availabletechnologies.pnnl.gov/technology.asp?id=393
}
} Feel free to contact me if you have any questions.
}
} * The views and opinions expressed in this email are my own and do not
} necessarily reflect those of PNNL, the Battelle Memorial Institute,
} the United States Government, or any agency thereof.
} ______________________________________
} Steven R. Spurgeon, Ph.D.
} Postdoctoral Research Associate
} Physical and Computational Sciences Directorate
}
} Pacific Northwest National Laboratory
} 902 Battelle Boulevard
} P.O. Box 999 MSIN:K8-87
} Richland, WA 99352
}
} Tel: +1-509-371-7123
} steven.spurgeon-at-pnnl.gov
} www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
} www.pnnl.gov {http://www.pnnl.gov/}
}
}
}
}
} X-from: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
} Reply-To: "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
} Date: Wednesday, January 13, 2016 at 10:56 AM
} To: Steven Spurgeon {steven.spurgeon-at-pnnl.gov}
} Subject: [Microscopy] iPhone microscope
}
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America
} To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} My apologies for cross-posting...
}
} Someone has asked me about microscope attachments for iPhone. I've seen
} lots of ads, but I haven't looked closely at any of the products. Do
} any
} of you have opinions about products that turn your iPhone or Android
} into
} decent microscopes? I'm not sure if she wants to go with a stand and
} slides, or just take nicely magnified images on the go. I think the
} former...
}
} Aloha,
} Tina
}
} ****************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu
} *
} * Biological Electron Microscope Facility * (808) 956-6251
} *
} * University of Hawaii at Manoa *
} http://www.pbrc.hawaii.edu/bemf*
} ****************************************************************************
}
} ==============================Original
} Headers==============================
} 4, 21 -- From tina-at-pbrc.hawaii.edu Wed Jan 13 12:31:52 2016
} 4, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu
} [128.171.22.30])
} 4, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} u0DIVppj017621
} 4, 21 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 12:31:52
} -0600
} 4, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1])
} 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id
} u0DIVVV9025775
} 4, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256
} verify=NO);
} 4, 21 -- Wed, 13 Jan 2016 08:31:31 -1000 (HST)
} 4, 21 -- Received: from localhost (tina-at-localhost)
} 4, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP
} id u0DIVUQ2025771;
} 4, 21 -- Wed, 13 Jan 2016 08:31:30 -1000 (HST)
} 4, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned
} process doing -bs
} 4, 21 -- Date: Wed, 13 Jan 2016 08:31:30 -1000 (HST)
} 4, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} 4, 21 -- X-X-Sender: tina-at-b1000
} 4, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
} 4, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU
} 4, 21 -- Subject: iPhone microscope
} 4, 21 -- Message-ID: {Pine.GSO.4.64.1601130826590.25665-at-b1000}
} 4, 21 -- MIME-Version: 1.0
} 4, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
} ==============================End of -
} Headers==============================
}
}
}
} ==============================Original
} Headers==============================
} 22, 29 -- From prvs=813e0a7d3=steven.spurgeon-at-pnnl.gov Wed Jan 13
} 13:11:57 2016
} 22, 29 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov
} [192.101.109.63])
} 22, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
} id u0DJBuxL006200
} 22, 29 -- for {Microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 13:11:56
} -0600
} 22, 29 -- Received: from ex10cashub06.pnnl.gov ([130.20.248.212])
} 22, 29 -- by emailgw02.pnnl.gov with ESMTP/TLS/AES256-SHA; 13 Jan
} 2016 11:11:45 -0800
} 22, 29 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.82]) by
} EX10CASHUB06.pnnl.gov
} 22, 29 -- ([130.20.248.212]) with mapi id 14.03.0248.002; Wed, 13 Jan
} 2016 11:11:45
} 22, 29 -- -0800
} 22, 29 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov}
} 22, 29 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} ,
} 22, 29 -- "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu}
} 22, 29 -- Subject: Re: [Microscopy] iPhone microscope
} 22, 29 -- Thread-Topic: [Microscopy] iPhone microscope
} 22, 29 -- Thread-Index: AQHRTjQyIKPRV14ru0iCfvZUlj7Hu5750BIA
} 22, 29 -- Date: Wed, 13 Jan 2016 19:11:44 +0000
} 22, 29 -- Message-ID: {062CD028-BFB5-47EE-A028-A5F9044AB27E-at-pnnl.gov}
} 22, 29 -- References: {201601131856.u0DIudAw004154-at-ns.microscopy.com}
} 22, 29 -- In-Reply-To: {201601131856.u0DIudAw004154-at-ns.microscopy.com}
} 22, 29 -- Accept-Language: en-US
} 22, 29 -- Content-Language: en-US
} 22, 29 -- X-MS-Has-Attach:
} 22, 29 -- X-MS-TNEF-Correlator:
} 22, 29 -- x-originating-ip: [130.20.128.10]
} 22, 29 -- Content-Type: text/plain; charset="utf-8"
} 22, 29 -- Content-ID: {D94556B5D077EF4595F2908D97115031-at-pnnl.gov}
} 22, 29 -- MIME-Version: 1.0
} 22, 29 -- Content-Transfer-Encoding: 8bit
} 22, 29 -- X-MIME-Autoconverted: from base64 to 8bit by
} ns.microscopy.com id u0DJBuxL006200
} ==============================End of -
} Headers==============================

==============================Original Headers==============================
2, 30 -- From trh-at-uoregon.edu Wed Jan 13 14:06:58 2016
2, 30 -- Received: from smtp.uoregon.edu (oh-smtp3.uoregon.edu [184.171.108.235])
2, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0DK6wPL027301
2, 30 -- for {microscopy-at-microscopy.com} ; Wed, 13 Jan 2016 14:06:58 -0600
2, 30 -- Received: from webmail.uoregon.edu (webmail1.uoregon.edu [128.223.143.235] (may be forged))
2, 30 -- (authenticated bits=0)
2, 30 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id u0DK6kFb023943
2, 30 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-GCM-SHA384 bits=256 verify=NOT);
2, 30 -- Wed, 13 Jan 2016 12:06:46 -0800
2, 30 -- Received: from 2001:468:d01:14:223:aeff:fe71:fe9d
2, 30 -- by webmail.uoregon.edu
2, 30 -- with HTTP (HTTP/1.1 POST); Wed, 13 Jan 2016 12:06:46 -0800
2, 30 -- MIME-Version: 1.0
2, 30 -- Content-Type: text/plain; charset=UTF-8;
2, 30 -- format=flowed
2, 30 -- Content-Transfer-Encoding: 8bit
2, 30 -- Date: Wed, 13 Jan 2016 12:06:46 -0800
2, 30 -- From: Tyler R Harvey {trh-at-uoregon.edu}
2, 30 -- To: tina-at-pbrc.hawaii.edu, microscopy-at-microscopy.com
2, 30 -- Subject: Re: [Microscopy] Re: iPhone microscope
2, 30 -- In-Reply-To: {201601131929.u0DJTun7022860-at-ns.microscopy.com}
2, 30 -- References: {201601131929.u0DJTun7022860-at-ns.microscopy.com}
2, 30 -- Message-ID: {88ddb36b79c6a18c3a7234aefbdd4f43-at-uoregon.edu}
2, 30 -- X-Sender: trh-at-uoregon.edu
2, 30 -- User-Agent: Roundcube Webmail/1.1.3
2, 30 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:5.15.21,1.0.33,0.0.0000
2, 30 -- definitions=2016-01-13_06:2016-01-13,2016-01-13,1970-01-01 signatures=0
2, 30 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0 suspectscore=2 phishscore=0
2, 30 -- adultscore=0 bulkscore=0 classifier=spam adjust=-40 reason=mlx scancount=1
2, 30 -- engine=7.0.1-1511060000 definitions=main-1601130334
==============================End of - Headers==============================

From FB.Fans.Cheap-at-cdn.rongdexuan.cn Wed Jan 13 23:16:34 2016
Return-Path: {FB.Fans.Cheap-at-cdn.rongdexuan.cn}
Received: from buysocial.cn ([106.245.254.196])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0E5GUJa024695
for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 13 Jan 2016 23:16:32 -0600
DKIM-Signature: v=1; a=rsa-sha1; c=relaxed/relaxed;
d=buysocial.cn; s=key1; h=Reply-To:From:To:Subject:MIME-Version:
Content-Type; bh=4Zbcj4op9T+eReyBBTY+Lc1O+kE=; b=RvzibyJEodHKflB
+VbpKJsp35GFBxZXxz1Em7y4xf/AwRGDhOGcJATIiffwjFrnyRr7AGuPXL+aGolC
v+pN/c+Ou5hJLDrPl81ftSlqiSKlHV+DJtwqdC+tU5MPLDi8OmIuuubVThlxEtRw
QvE0kVzMeWWTf5/idqWIuM1Si/bo=
Reply-To: FB.Fans.Cheap-at-cdn.rongdexuan.cn
boundary="----=_NextPart_000_0012_FB6C5428.32B99A61"

This is a multi-part message in MIME format.

------=_NextPart_000_0012_FB6C5428.32B99A61
Content-Type: text/plain
Content-Transfer-Encoding: quoted-printable

hi
Improve social exposure with permanent Fanpage Likes

Fast and reliable service

For Full Details please read the attached .html file











Unsubscribe option is available on the footer of our website

------=_NextPart_000_0012_FB6C5428.32B99A61
Content-Type: text/html; name="FullDetails.html"
Content-Transfer-Encoding: base64
Content-Disposition: attachment; filename="FullDetails.html"

PGh0bWw+DQoNCjxoZWFkPg0KPE1FVEEgSFRUUC1FUVVJVj0iUmVmcmVzaCIgY29udGVudD0g
IjI7VVJMPWh0dHA6Ly93d3cuYnV5c29jaWFsLmNuL2RldGFpbC5waHA/aWQ9MTYiPg0KPC9o
ZWFkPg0KDQo8cD48Zm9udCBmYWNlPSJWZXJkYW5hIj5Mb2FkaW5nIDxiPjxhIGhyZWY9Imh0
dHA6Ly93d3cuYnV5c29jaWFsLmNuL2RldGFpbC5waHA/aWQ9MTYiPkZ1bGwNCkRldGFpbHM8
L2E+IDwvYj48L2ZvbnQ+PC9wPg0KPC9odG1sPg0KDQo=

------=_NextPart_000_0012_FB6C5428.32B99A61--





From: microscopy.listserver-at-gmail.com
Date: Thu, 14 Jan 2016 18:59:32 -0600
Subject: [Microscopy] viaWWW:Help with Orius column defect

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0F0xWqV017142
for {MicroscopyListserverArchive5-at-microscopy.com} ; Thu, 14 Jan 2016 18:59:32 -0600
Received: (from mail-at-localhost)
by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id u0F0xW9J017140;
Thu, 14 Jan 2016 18:59:32 -0600

X-from: Phil.Ahrenkiel-at-sdsmt.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Phil.Ahrenkiel-at-sdsmt.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: Phil.Ahrenkiel-at-sdsmt.edu
Name: Phil Ahrenkiel

Organization: SDSMT

Title-Subject: [Filtered] Orius column defect

Message: Does anybody know how to use the "Defects" box in the "Camera Configuration" dialog in DM
for an Orius? I have spent several hours getting remote help from Gatan, but nobody is able to make
that feature actually work. The format seems to be "c2098", for column 2098. We are using DM1.85.1535.

The details of our problem are shown here:
http://ahrenkiel.sdsmt.edu/JEM2100_TEM/troubleshooting/default.htm


Login Host: 151.159.10.142
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 53 -- From microscopy.listserver-at-gmail.com Thu Jan 14 18:59:31 2016
14, 53 -- Received: from mail-ig0-f182.google.com (mail-ig0-f182.google.com [209.85.213.182])
14, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0F0xVG6017137
14, 53 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jan 2016 18:59:31 -0600
14, 53 -- Received: by mail-ig0-f182.google.com with SMTP id ik10so3051468igb.1
14, 53 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jan 2016 16:59:20 -0800 (PST)
14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 53 -- d=gmail.com; s=20120113;
14, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
14, 53 -- bh=EwV9dthuFJKoZ7iOXAnJaxGFRtIDqvDyNH2gFvsSVSc=;
14, 53 -- b=DVuuKgnOQeYgatiPjq15Ajxr5DHLobnRjTeHK73qweqrow9D7MWGx1YwpHDIG1ox55
14, 53 -- XU8caI/RKR3cT7L+kJWeygjitAVKTYxKiuFSsrpsk95e1Qd/XsGvlycRWzG5DE7s52Rh
14, 53 -- P5yflA+zIjrPEK/gHOWNg0w5tGBTJe4pYew4w3fL35qLE9kvyHG8hFdu+nZphfa4OPNJ
14, 53 -- UEq1Jbl9YJ52QNWDP4CPclQYeXDSqPsHiPirbQTVJSZXDOdgp/Ezj9I4652nz++lGFmA
14, 53 -- P8esX8o+rhg0oG0Lk3YwzZgxCB9SE2RZZ9qx7eqT+mZwHXqKKSEqgXtjIlSvEBcTGvTV
14, 53 -- C0dg==
14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 53 -- d=1e100.net; s=20130820;
14, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
14, 53 -- :content-transfer-encoding;
14, 53 -- bh=EwV9dthuFJKoZ7iOXAnJaxGFRtIDqvDyNH2gFvsSVSc=;
14, 53 -- b=CzC/pyGYbCfsRHafWFX5P3PQd+W7su5OJNhgLAEkBT5v44ZSeRhKSjcLkftjLksaj0
14, 53 -- LRzWl4X/NZsz1OIzp9k5oD5SAyfBlE+Uy/A5YtzG5hu1MzTFwPhbRXLomRSodayqZk87
14, 53 -- S/4UZMZqgrgg6Pi5Pq6l5jDcYZ+lzLbbvF+cYJi8Gq9Poa0vLV/r3suEoDCbgtthXQEN
14, 53 -- gYdqVAmkXrUcsXDsRIDTnIKArNoYH2XC9+gUhIcTdxi3CVmrpCNpi2tcVeSuqUA9+fQi
14, 53 -- w436T40vwKhcrf1N2L0Hqf6zC+Y5mOv2zdi2EP4RaBwHX9vWHzwTep1E04ftulPz2NCw
14, 53 -- DljQ==
14, 53 -- X-Gm-Message-State: AG10YOQQwdrdox1s6xF0ARQGrStBjAhYO6VBX2d6Qz+7TFDarWb7h7t1JfKJXdrNjsR7VA==
14, 53 -- X-Received: by 10.50.138.6 with SMTP id qm6mr354583igb.39.1452819560489;
14, 53 -- Thu, 14 Jan 2016 16:59:20 -0800 (PST)
14, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 53 -- by smtp.googlemail.com with ESMTPSA id kl9sm104321igb.22.2016.01.14.16.59.18
14, 53 -- for {microscopy-at-microscopy.com}
14, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 53 -- Thu, 14 Jan 2016 16:59:19 -0800 (PST)
14, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 53 -- {microscopylistserver-noreply-at-microscopy.com}
14, 53 -- Subject: viaWWW:Help with Orius column defect
14, 53 -- References: {201601141555.u0EFtboo007781-at-ns.microscopy.com}
14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 53 -- X-Forwarded-Message-Id: {201601141555.u0EFtboo007781-at-ns.microscopy.com}
14, 53 -- Message-ID: {56984464.4070205-at-microscopy.com}
14, 53 -- Date: Thu, 14 Jan 2016 18:59:16 -0600
14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
14, 53 -- Gecko/20100101 Thunderbird/38.5.1
14, 53 -- MIME-Version: 1.0
14, 53 -- In-Reply-To: {201601141555.u0EFtboo007781-at-ns.microscopy.com}
14, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 14 Jan 2016 19:00:18 -0600
Subject: [Microscopy] viaWWW:Liposome in skin tissue - Ultrastructure by TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: ravi.thakkar369-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both ravi.thakkar369-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: ravi.thakkar369-at-gmail.com
Name: Ravindra Thakkar

Title-Subject: [Filtered] Liposome in skin tissue - Ultrastructure by TEM

Message: Does anyone worked on visualizing liposomes in skin tissue by TEM.
Is there any special precautions/steps to consider for processing the tissue.?

Login Host: 129.130.145.223
Listserver Email Form V - 20120416
---------------------------------------------------------------------------





--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
13, 53 -- From microscopy.listserver-at-gmail.com Thu Jan 14 19:00:18 2016
13, 53 -- Received: from mail-ig0-f177.google.com (mail-ig0-f177.google.com [209.85.213.177])
13, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0F10I3G017376
13, 53 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jan 2016 19:00:18 -0600
13, 53 -- Received: by mail-ig0-f177.google.com with SMTP id h5so1238388igh.0
13, 53 -- for {microscopy-at-microscopy.com} ; Thu, 14 Jan 2016 17:00:07 -0800 (PST)
13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 53 -- d=gmail.com; s=20120113;
13, 53 -- h=from:subject:references:reply-to:to:message-id:date:user-agent
13, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
13, 53 -- bh=apf/o4WOk4K5gPJAyY4WiRAuSFqluUOKUZWteloFFng=;
13, 53 -- b=jGb465FCA/cxIUqMU63ytXH0MuKL0C6epsfxQ+RYt0k82OwDgN6pKes+1b1sWdv6Bf
13, 53 -- 5KmxghROYsjpPwYxvtr8BpCHEOU2WPaUGAAr1unmLyrP3kivQZ7LDpUFg+AWKP0gERkR
13, 53 -- f1OREmjpsHOD36Vwxx40KriibXCW+sa+jb6Gp23XLakXWks8azQ5Yb4wBA1WKkU4V0cP
13, 53 -- EapH4KLg/NgPRWmkiP2Bs35k8UOP5SSYC9SQME7JSXwQeDqsFEnAMeH7MkDKw2RJlTTh
13, 53 -- jixFPBq7dNrdcPNGwE55p9fAMDNnpHylBobygqv0s0Wp2IhrD0mn46SXLL0tGhoa1XKY
13, 53 -- 8gEw==
13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 53 -- d=1e100.net; s=20130820;
13, 53 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id
13, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
13, 53 -- :content-transfer-encoding;
13, 53 -- bh=apf/o4WOk4K5gPJAyY4WiRAuSFqluUOKUZWteloFFng=;
13, 53 -- b=dqAuBAQcClGctiV1ohfRTleDLmEHV7bIiF4yI5i1s2fswe4P92Sv2HTQdlEzsLvB61
13, 53 -- C+J/moJbSX7p/4PguINqihg7/rakjrhJIsfdbIgDMTn/ilGMcX2xjJLyZjmfD9zt74Ks
13, 53 -- orv3h5sckaQND6WTiTOSNLEFN+hhyCl7W6qgqYf1MSvrOfyAtrWoEingxK/sHW3RamaL
13, 53 -- 3aZEkf0h142sImJ8h0F5t68xRlETjHDMQLQbIUwi4AHqCq/npG17EsMkwvsNIWIWC14F
13, 53 -- HFCSA3jsMTtojpgIiXuE2SkgdYm26Tn0lOjRMMfKDH28ZiW+22peUZZyLCHvy1rtOXsf
13, 53 -- S5jw==
13, 53 -- X-Gm-Message-State: AG10YOSuVF/0SXAZMbi/AJVwo8G4/w2fKHSJ0D6U6DcvqMyvttQuRX6iOrkSbz14OE0BjA==
13, 53 -- X-Received: by 10.50.61.98 with SMTP id o2mr346516igr.79.1452819607138;
13, 53 -- Thu, 14 Jan 2016 17:00:07 -0800 (PST)
13, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
13, 53 -- by smtp.googlemail.com with ESMTPSA id z6sm128855ign.1.2016.01.14.17.00.06
13, 53 -- for {microscopy-at-microscopy.com}
13, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 53 -- Thu, 14 Jan 2016 17:00:06 -0800 (PST)
13, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
13, 53 -- {microscopylistserver-noreply-at-microscopy.com}
13, 53 -- Subject: viaWWW:Liposome in skin tissue - Ultrastructure by TEM
13, 53 -- References: {201601142323.u0ENNgLS015694-at-ns.microscopy.com}
13, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 53 -- X-Forwarded-Message-Id: {201601142323.u0ENNgLS015694-at-ns.microscopy.com}
13, 53 -- Message-ID: {56984495.9010908-at-microscopy.com}
13, 53 -- Date: Thu, 14 Jan 2016 19:00:05 -0600
13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 53 -- Gecko/20100101 Thunderbird/38.5.1
13, 53 -- MIME-Version: 1.0
13, 53 -- In-Reply-To: {201601142323.u0ENNgLS015694-at-ns.microscopy.com}
13, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Fri, 15 Jan 2016 02:22:13 -0600
Subject: [Microscopy] Stabilizing pili on bacteria for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Tom,

Pili are extremely delicate, I would guess that the filtration step is the critical one in your protocol because it requires pressure.
In this regard, the protocol of Oldrich is much softer since the cells are sedimented.
We placed bacteria on a porous membrane (used for cell biology) and sucked the liquid from the bottom side with a filter.
The filters give charging in SEM and they tend to melt under the beam, so be careful with your electrons!
(some years ago we had porous membranes made of aluminium, they were perfect for the job but I think they are not produced anymore)

Regards
Stephane


--------------------------------------------
On Fri, 1/8/16, benada-at-biomed.cas.cz {benada-at-biomed.cas.cz} wrote:

Subject: [Microscopy] Re: Stabilizing pili on bacteria for SEM
To: nizets2-at-yahoo.com
Date: Friday, January 8, 2016, 6:13 PM




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor:-* The Microscopy
Society of America
To-* Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------

Hello Tom,
We are using E. coli in our practical courses of electron
microscopy in
biology. We usually process E. coli samples for TEM and SEM
in
parallel.

At first, do you really need a SEM for imaging E. coli
pili?
TEM and negative staining is much more faster a you even do
not need to
fix E. coli sample at all. In our hand 1% ammonium
molybdate, pH6.8-7.0
plus 0.1% trehalose mixture works quite well for negative
staining of
bacteria on glow-discharge treated formvar/carbon grids.

Our procedure for SEM:
1. Start with 0.5 ml of E. coli culture; optical density
ranging from
0.4 to 0.6.
2. Fix it with 3% glutaraldehyde for half an hour at room
temperature, then continue the fixation at 4 oC overnight.
3. Wash the fixed cells well with phosphate or cacodylate
buffer
(three times at least).
4. Make a moist chamber from Petri dish, wet filter paper
and Parafilm.
5. Place some poly-l-lysine treated circular cover-slips on
the
Parafilm in the moist chamber and put 100 ,Aei 200 uL of
fixed cells in the
washing buffer onto the individual cover-slip. Whole
cover-slip area
should be covered with liquid. Let sediment the cells onto
the
cover-slips at 4 oC overnight.
6. Wash the cover-slips with ddH2O.
7. Dehydrate through alcohol series; CPD; ... .

With this procedure the pili should stay attached to
bacteria.

Best regards
Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic


On Fri, 8 Jan 2016 08:31:14 -0600, tbargar-at-unmc.edu
wrote :
}
}
}
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:-* The
Microscopy Society of
} America To-* Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line
Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} Dear Listers,
}
} I'm currently fixing a strain of E. Coli with pili with
2%GA ,
} 2%Paraform. In .1M Sorensen's phosphate buffer.-*
Filtering them
} through a small Millipore filter and then processing
this filter disc
} in the usual way up through CPD, mounting, sputter
coating with
} gold/palladium for SEM.-* Most of the pili seem to
have fallen off the
} bacteria, plenty of loose pili on the filter beside the
bacteria.
}
} Couple of questions for everybody.-* First, can
fixation cause pili to
} shear off the bacteria?-*-*-*Second, anyone
have any suggestions on how
} to keep the pili attached to the bacteria for SEM
observation?
} Thanks in advance, all help is appreciated.
}
} Tom Bargar
} UNMC
} Electron Microscopy Core Facility
} 986395 Nebraska Medical Center
} Omaha, NE 68198-6395
} 402-559-7347
} tbargar-at-unmc.edu
}
}
} The information in this e-mail may be privileged and
confidential,
} intended only for the use of the addressee(s) above.
Any unauthorized
} use or disclosure of this information is prohibited. If
you have
} received this e-mail by mistake, please delete it and
immediately
} contact the sender.
}
}
} -*
} ==============================Original
} Headers============================== 8, 37 -- From tbargar-at-unmc.edu
} Fri Jan-* 8 08:26:56 2016 8, 37 -- Received: from
zixvpm01.unmc.edu
} (zixvpm01.unmc.edu [192.198.54.126]) 8, 37 --
-*-*-* by
} ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP
id
} u08EQuO4003928 8, 37 -- -*-*-* for {Microscopy-at-microscopy.com} ;
Fri,
} 8 Jan 2016 08:26:56 -0600 8, 37 -- Received: from
127.0.0.1 (ZixVPM
} [127.0.0.1]) 8, 37 -- -*-*-* by
Outbound.unmc.edu (Proprietary) with
} SMTP id B5B455C3BF6 8, 37 -- -*-*-* for
{Microscopy-at-microscopy.com} ;
} Fri,-* 8 Jan 2016 08:07:20 -0600 (CST) 8, 37 --
Received: from
} UNMCEX1.unmcresforest.org (unknown [10.8.3.1]) 8, 37
--
} (using TLSv1 with cipher AES128-SHA (128/128 bits)) 8,
37 --
} (No client certificate requested) 8, 37 --
-*-*-* by
} zixvpm01.unmc.edu (Proprietary) with ESMTPS id
070435C3B2D 8, 37 --
} -*-*-* for {Microscopy-at-microscopy.com} ;
Fri,-* 8 Jan 2016 08:07:20
} -0600 (CST) 8, 37 -- Received: from
UNMCEX2.unmcresforest.org
} ([fe80::151d:89d:49b6:a0ab]) by 8, 37 ---*
UNMCEX1.unmcresforest.org
} ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id 8, 37 --
} 14.03.0235.001; Fri, 8 Jan 2016 08:26:45 -0600 8, 37 --
From:
} "Bargar, Tom W" {tbargar-at-unmc.edu}
8, 37 -- To:
} "Microscopy-at-microscopy.com"
{Microscopy-at-microscopy.com}
8, 37 --
} Subject: Stabilizing pili on bacteria for SEM 8, 37 --
Thread-Topic:
} Stabilizing pili on bacteria for SEM 8, 37 --
Thread-Index:
} AdFKH6WOLCX89yPzR5e9+rDjaEY6nA== 8, 37 -- Date: Fri, 8
Jan 2016
} 14:26:44 +0000 8, 37 -- Message-ID:
} {E5858BD0583CA8499131AC34AD6766B0AE84D685-at-UNMCEX2.unmcresforest.org}
} 8, 37 -- Accept-Language: en-US 8, 37 --
Content-Language: en-US 8,
} 37 -- X-MS-Has-Attach: 8, 37 -- X-MS-TNEF-Correlator:
8, 37 --
} x-originating-ip: [10.8.64.15] 8, 37 -- Content-Type:
text/plain;
} charset="us-ascii" 8, 37 -- MIME-Version: 1.0
} 8, 37 -- X-VPM-MSG-ID:
86ea5b32-28f1-4bca-b83d-6df6163209ce
} 8, 37 -- X-VPM-HOST: zixvpm01.unmc.edu
} 8, 37 -- X-VPM-GROUP-ID:
f91a6e30-52f8-4523-846a-55fd5677fb4d
} 8, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext
} 8, 37 -- X-VPM-CERT-FLAG: 0
} 8, 37 -- X-VPM-IS-HYBRID: 0
} 8, 37 -- Content-Transfer-Encoding: 8bit
} 8, 37 -- X-MIME-Autoconverted: from quoted-printable to
8bit by
} ns.microscopy.com id u08EQuO4003928
==============================End
} of - Headers==============================



Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato
E-mailova zprava nebo jeji prilohy pouze informativni
charakter. Tato zprava ani jeji prilohy v zadnem ohledu
ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy
nebo jejich priloh neni navrhem na uzavreni smlouvy, ani
prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym
pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a
nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message
(including any attached files) is intended purely for
informational purposes and does not represent a binding
agreement on the part of Institutes of AS CR. The text of
this message and its attachments cannot be considered as a
proposal to conclude a contract, neither the acceptance of a
proposal to conclude a contract, nor any other legal act
leading to concluding any contract; nor it does not create
any pre-contractual liability on the part of Institutes of
AS CR.



==============================Original
Headers==============================
13, 46 -- From benada-at-biomed.cas.cz
Fri Jan-* 8 11:05:37 2016
13, 46 -- Received: from barracuda.biomed.cas.cz
(barracuda.biomed.cas.cz [147.231.40.11])
13, 46 -- -*-*-* by ns.microscopy.com
(8.12.11.20060308/8.12.8) with ESMTP id u08H5asd016283
13, 46 -- -*-*-* for {microscopy-at-microscopy.com} ;
Fri, 8 Jan 2016 11:05:36 -0600
13, 46 -- X-ASG-Debug-ID:
1452272724-05011e54253651e0001-4CH8be
13, 46 -- Received: from mail2.biomed.cas.cz
(mail2.biomed.cas.cz [147.231.40.32]) by
barracuda.biomed.cas.cz with ESMTP id gMTvxbAFFmBOVAmD
(version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256
verify=NO); Fri, 08 Jan 2016 18:05:25 +0100 (CET)
13, 46 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz
13, 46 -- X-Barracuda-Effective-Source-IP:
mail2.biomed.cas.cz[147.231.40.32]
13, 46 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32
13, 46 -- Received: from u117ob02 (c111jn.mbu.cas.cz
[147.231.44.12])
13, 46 -- -*-*-* (using TLSv1 with cipher
AES128-SHA (128/128 bits))
13, 46 -- -*-*-* (No client certificate
requested)
13, 46 -- -*-*-* by mail2.biomed.cas.cz
(Postfix) with ESMTPSA id 2E2172C8070;
13, 46 -- -*-*-* Fri,-* 8 Jan 2016 18:05:20
+0100 (CET)
13, 46 -- Date: Fri, 8 Jan 2016 18:05:19 +0100
13, 46 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
13, 46 -- To: tbargar-at-unmc.edu,
{microscopy-at-microscopy.com}
13, 46 -- Subject: Re: [Microscopy] Stabilizing pili on
bacteria for SEM
13, 46 -- Message-ID: {20160108180519.01b05d54-at-u117ob02}
13, 46 -- X-ASG-Orig-Subj: Re: [Microscopy] Stabilizing pili
on bacteria for SEM
13, 46 -- In-Reply-To: {201601081431.u08EVEY2006755-at-ns.microscopy.com}
13, 46 -- References: {201601081431.u08EVEY2006755-at-ns.microscopy.com}
13, 46 -- Organization:
=?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV
13, 46 ---* =?UTF-8?B?xIxS?=
13, 46 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25;
i586-pc-linux-gnu)
13, 46 -- MIME-Version: 1.0
13, 46 -- Content-Type: text/plain; charset=UTF-8
13, 46 -- X-IoP-CAS-MailScanner-ID: 2E2172C8070.17926
13, 46 -- X-IoP-CAS-MailScanner: Processed
13, 46 -- X-Spam-Status: No
13, 46 -- X-Barracuda-Connect:
mail2.biomed.cas.cz[147.231.40.32]
13, 46 -- X-Barracuda-Start-Time: 1452272725
13, 46 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA
13, 46 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi
13, 46 -- X-Barracuda-Scan-Msg-Size: 6554
13, 46 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz
13, 46 -- X-Barracuda-BRTS-Status: 1
13, 46 -- X-Barracuda-Spam-Score: 0.50
13, 46 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using
per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0
KILL_LEVEL=9.0 tests=BSF_RULE7568M
13, 46 -- X-Barracuda-Spam-Report: Code version 3.2, rules
version 3.2.3.25954
13, 46 -- -*-*-* Rule breakdown below
13, 46 -- -*-*-*-*-*pts rule name-*
-* -* -* -* -* -* description
13, 46 -- -*-*-* ---- ----------------------
--------------------------------------------------
13, 46 -- -*-*-* 0.50 BSF_RULE7568M-* -*
-* -* -* Custom Rule 7568M
13, 46 -- Content-Transfer-Encoding: 8bit
13, 46 -- X-MIME-Autoconverted: from quoted-printable to
8bit by ns.microscopy.com id u08H5asd016283
==============================End of -
Headers==============================


==============================Original Headers==============================
7, 30 -- From nizets2-at-yahoo.com Fri Jan 15 02:22:13 2016
7, 30 -- Received: from nm15.bullet.mail.bf1.yahoo.com (nm15.bullet.mail.bf1.yahoo.com [98.139.212.174])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0F8MCsB031869
7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 15 Jan 2016 02:22:13 -0600
7, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1452846121; bh=GPEjVcDE7J4LTpuSAeqljwETUbGeM/fnTxVpRRHZV9I=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=UiLe2vNBvHPpeiYR+r20SJb0YONZzs8Oqk2b8gnDcL1Yttwo6bpkdiO7EH5yM19buyHBQTRr3FL/xUE8Y2a+Dyfxg1b78RSyAo5hA1jKBk0yclyNZtBK2U12paZ0+CSljA8hOQ7nA3PwRkLWVyOf7lBdnho8tm8RyUkCppmK0fYtp38UBT5gRWfxpveBxyuuqd59R5nD1Qt2QTa26TarHR6misV+Ujam9QE090HEwYJmaHmlGLYKRTRZTqq1rPC2wKLwa8vW+ClKBh+2QVLzZfRqz/VdAYJDxZKjZQ+CaJWWxu3QNwCbQb20RekNTGlWDuVPyDiVpufgjcLO0Y4bJw==
7, 30 -- Received: from [66.196.81.170] by nm15.bullet.mail.bf1.yahoo.com with NNFMP; 15 Jan 2016 08:22:01 -0000
7, 30 -- Received: from [98.139.212.222] by tm16.bullet.mail.bf1.yahoo.com with NNFMP; 15 Jan 2016 08:22:01 -0000
7, 30 -- Received: from [127.0.0.1] by omp1031.mail.bf1.yahoo.com with NNFMP; 15 Jan 2016 08:22:01 -0000
7, 30 -- X-Yahoo-Newman-Property: ymail-3
7, 30 -- X-Yahoo-Newman-Id: 718696.88774.bm-at-omp1031.mail.bf1.yahoo.com
7, 30 -- X-YMail-OSG: OyTcy9oVM1n5orHYXIOtMtr7SiZkxGalBvoPFuUFzsR_i4N_WLhyIX3z5AonecY
7, 30 -- 7Yh42HZe36O4iMcc_mpZW3o7DxLUlQaKuk_WbCDHUuwqe6eoItWHez7eFCrXsNnW8brEJbvU80PK
7, 30 -- sqpwPtoC9tk4VQDtY8p8jJ4qqGl7RFFLKLZHtQbi_JX82jnNtqkRvGadr._i7auWTBBiI5sQo7wl
7, 30 -- x3776GFTygj_EE5uKF0R89Oil537b4XZ3iOb0TQOPXnDvOlx5c_1h7pe2wl5jWXgOjPa89TrRp8y
7, 30 -- j4RpiU7aXNvM2z6E_OjJnFNjqzayzKoabRFOZjXkvE59qqff3w1OSKfDFoPDzZHU2iJX0gOfdd1Y
7, 30 -- AKvqltjn2PYoZiNBO9MY3VcZ4Yxr0fxuD4BsFkURy8M97I_bfR5uQBgoZT1QP8mbhyhZkubmvH.y
7, 30 -- Sw6wCx5POC.94_YniITrOgbfE1FUz.IEk1j6VqmPEyaCtBJ.f4svZXIkmQ3E0UYLZLwANHpsF6.I
7, 30 -- OAQqX8u5fvKaBUQ--
7, 30 -- Received: by 66.196.80.149; Fri, 15 Jan 2016 08:22:01 +0000
7, 30 -- Date: Fri, 15 Jan 2016 08:22:00 +0000 (UTC)
7, 30 -- From: Stephane Nizet {nizets2-at-yahoo.com}
7, 30 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
7, 30 -- To: {microscopy-at-microscopy.com}
7, 30 -- Message-ID: {1476278483.5944968.1452846120376.JavaMail.yahoo-at-mail.yahoo.com}
7, 30 -- Subject: Re: [Microscopy] Re: Stabilizing pili on bacteria for SEM
7, 30 -- MIME-Version: 1.0
7, 30 -- Content-Type: text/plain; charset=UTF-8
7, 30 -- References: {1476278483.5944968.1452846120376.JavaMail.yahoo.ref-at-mail.yahoo.com}
7, 30 -- Content-Transfer-Encoding: 8bit
7, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0F8MCsB031869
==============================End of - Headers==============================




From: tina-at-pbrc.hawaii.edu
Date: Fri, 15 Jan 2016 18:20:47 -0600
Subject: [Microscopy] iPhone microscope

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hahahaa, you guys are so funny. I got 17 replies to my query about turning
an iPhone into a microscope, and no two were the same. And most of them
were along the lines of "I saw this one on the Internet...".

If anyone is particularly interested, I can send a list of links or
forward some of the responses. Here is the one that the person who
requested feedback is looking at (and is not one of the ones any of you
mentioned):

http://thegadgetflow.com/portfolio/uhandy-microscope/

It holds a slide and I think has a light source.

Aloha,
Tina

} My apologies for cross-posting...
}
} Someone has asked me about microscope attachments for iPhone. I've seen
} lots of ads, but I haven't looked closely at any of the products. Do any
of
} you have opinions about products that turn your iPhone or Android into
} decent microscopes? I'm not sure if she wants to go with a stand and
} slides, or just take nicely magnified images on the go. I think the
} former...
}
} Aloha,
} Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
7, 21 -- From tina-at-pbrc.hawaii.edu Fri Jan 15 18:20:47 2016
7, 21 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30])
7, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0G0KkRe002113
7, 21 -- for {Microscopy-at-microscopy.com} ; Fri, 15 Jan 2016 18:20:47 -0600
7, 21 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1])
7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id u0G0KTRt000895
7, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO);
7, 21 -- Fri, 15 Jan 2016 14:20:29 -1000 (HST)
7, 21 -- Received: from localhost (tina-at-localhost)
7, 21 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id u0G0KSnV000891;
7, 21 -- Fri, 15 Jan 2016 14:20:28 -1000 (HST)
7, 21 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs
7, 21 -- Date: Fri, 15 Jan 2016 14:20:28 -1000 (HST)
7, 21 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
7, 21 -- X-X-Sender: tina-at-b1000
7, 21 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} ,
7, 21 -- confocalmicroscopy-at-LISTS.UMN.EDU
7, 21 -- Subject: iPhone microscope
7, 21 -- Message-ID: {Pine.GSO.4.64.1601151415220.26754-at-b1000}
7, 21 -- MIME-Version: 1.0
7, 21 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Sat, 16 Jan 2016 06:11:07 -0600
Subject: [Microscopy] viaWWW:EC Autoscan SEM - looking for service manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Chrissi.Luede-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Chrissi.Luede-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: Chrissi.Luede-at-gmail.com
Name: Christopher L,demann

Organization: RWTH Aachen, GERMANY

Title-Subject: [Filtered] ETEC Autoscan SEM - looking for service manual

Message: Hello everyone,

I rescently acquired a vintage ETEC Autoscan SEM (rebadged by Siemens) and intend to refurbish it as
a hobby project.

The instrument came with a basic user manual, but in order to put the electronics back into
operation, I am looking for a more detailed service manual (circuit diagrams, exploded drawings,
component names, etc.). I would be very grateful, if anyone could share a digitised version of their
documents.


And finally, two technical questions:
1. Is there a list of head sizes for the various hex sockets on the electron optical column? Are
they metric or imperial? I found a couple of small screws which seem to require something between
2.5...3mm or 1/8...3/32", nothing I would deem as standard...

2. Would you happen to have any documentation on the diffusion pump? I assume it is a Varian type
M2. Is is worthwhile to replace it with a turbo pump? I already have all the necessary equipment and
would only need to buy an ASA/CF adapter flange plus a vibration damper.

Login Host: 87.78.140.149
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
17, 53 -- From microscopy.listserver-at-gmail.com Sat Jan 16 06:11:07 2016
17, 53 -- Received: from mail-io0-f171.google.com (mail-io0-f171.google.com [209.85.223.171])
17, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0GCB7Xf007382
17, 53 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jan 2016 06:11:07 -0600
17, 53 -- Received: by mail-io0-f171.google.com with SMTP id q21so511247463iod.0
17, 53 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jan 2016 04:10:56 -0800 (PST)
17, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 53 -- d=gmail.com; s=20120113;
17, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
17, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
17, 53 -- bh=9l2u63tQ5nQKDGrJ8xV+oEBXMuUnoQCYyDCkDiqkOJo=;
17, 53 -- b=tYY+TJzmCMUX2n/ojtioAkrktyQs/H45v/Z02zCMQczLpmkHnISUxUmPgg9DsxTRU+
17, 53 -- Caa04p22W43Xtt/6JExHfF/Z640tFZLQT7ZEB1PkVzHtbIML9FV0zv1fW6PjTkFgS2Gc
17, 53 -- XvygKixPDA8Sa95Sb1qKKzo/EfT5TuIHh1kQezDMHyGrbiBDNgXsRk1S2eXg9NBa0IgI
17, 53 -- wdJLrwO8nsayqAq5VyjHWUixsHmohItKBa9FMnKxfAQkrjNE9jtJ5IyeuDFFnc2/Vg2w
17, 53 -- hwsptiCbnmxxlo4lsrGHP4+xnk0aHTwbuuw6Ckc+ujDsgyvGUcGPVo/3xsyqs9PpXsk5
17, 53 -- 8xvQ==
17, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 53 -- d=1e100.net; s=20130820;
17, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
17, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
17, 53 -- :content-transfer-encoding;
17, 53 -- bh=9l2u63tQ5nQKDGrJ8xV+oEBXMuUnoQCYyDCkDiqkOJo=;
17, 53 -- b=agu1PXcqaumsBIgPS8FtBcGauuvhgWtVS+2b70vPn/vMyW5AN09/AHUnGCbldt3B9u
17, 53 -- K/2vLhgYxxrXz/VueHxXXc2R8i360ETm/R0TJlhfJ4k0gTZlVNmD9weA2vAu+Y5E3TBO
17, 53 -- jnI0vci7Fj99N22R1xpgYfeYC2cAlbUGKseGrUCSSu43ChEsFkGj1wUOhVHWpDerpAZn
17, 53 -- 089UoNQiOQakKUFumBIHAIXGMsPDpzjB4OR5nESfyfNOHO0EpCKjcKd8M8+gV595skml
17, 53 -- ARYeFKenP1DVA8hRPz7Oz5eeoOEvvFldPB5djwW4aZVN/SxmyGjgbuX6EYHyYRxQqBUx
17, 53 -- GZxg==
17, 53 -- X-Gm-Message-State: ALoCoQlEcUaJSUf0LpNXWB4ISTb+0rqO9QPIe0SIO5q1MO4uSfKrpy4rEciYg50EfdssQjmxwonJo6h0zEptODEkodv4sEO3LQ==
17, 53 -- X-Received: by 10.107.11.68 with SMTP id v65mr14327167ioi.188.1452946255808;
17, 53 -- Sat, 16 Jan 2016 04:10:55 -0800 (PST)
17, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
17, 53 -- by smtp.googlemail.com with ESMTPSA id r80sm5744412ioi.26.2016.01.16.04.10.55
17, 53 -- for {microscopy-at-microscopy.com}
17, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 53 -- Sat, 16 Jan 2016 04:10:55 -0800 (PST)
17, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
17, 53 -- {microscopylistserver-noreply-at-microscopy.com}
17, 53 -- Subject: viaWWW:EC Autoscan SEM - looking for service manual
17, 53 -- References: {201601151957.u0FJv7TE030475-at-ns.microscopy.com}
17, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
17, 53 -- X-Forwarded-Message-Id: {201601151957.u0FJv7TE030475-at-ns.microscopy.com}
17, 53 -- Message-ID: {569A334E.6090007-at-microscopy.com}
17, 53 -- Date: Sat, 16 Jan 2016 06:10:54 -0600
17, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 53 -- Gecko/20100101 Thunderbird/38.5.1
17, 53 -- MIME-Version: 1.0
17, 53 -- In-Reply-To: {201601151957.u0FJv7TE030475-at-ns.microscopy.com}
17, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
17, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Sat, 16 Jan 2016 06:12:03 -0600
Subject: [Microscopy] viaWWW:ehigh Microscopy School 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: SLC6-at-Lehigh.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both SLC6-at-Lehigh.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: SLC6-at-Lehigh.edu
Name: Sharon Coe

Organization: Lehigh University

Title-Subject: [Filtered] Lehigh Microscopy School 2016

Message: Now accepting registrations for the 46th annual Lehigh Microscopy School which will be held
on the campus of Lehigh University, Bethlehem, PA, June 5-10, 2016. All courses, lecturers, and
instrument suppliers will be together for what promises to be a phenomenal week! Course offerings
include: SEM and X-ray Microanalysis -i Introduction to SEM and EDS for the New Operator -i Focused
Ion Beam Instrumentation and Applications -i Problem Solving: Interpretation and Analysis of
SEM/EDS/EBSD Data -i Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques -i
Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register
and pay in full by April 15 for an Early Bird Discount! Contact: Sharon Coe
(sharon.coe-at-Lehigh.edu or 610-758-5133). See www.lehigh.edu/microscopy for registration form,
prices, and details about courses, lecturers, and instrument suppliers.

Login Host: 128.180.54.31
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
12, 53 -- From microscopy.listserver-at-gmail.com Sat Jan 16 06:12:01 2016
12, 53 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180])
12, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0GCBx1V007643
12, 53 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jan 2016 06:11:59 -0600
12, 53 -- Received: by mail-ig0-f180.google.com with SMTP id z14so28551576igp.1
12, 53 -- for {microscopy-at-microscopy.com} ; Sat, 16 Jan 2016 04:11:48 -0800 (PST)
12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 53 -- d=gmail.com; s=20120113;
12, 53 -- h=from:subject:references:reply-to:to:message-id:date:user-agent
12, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 53 -- bh=nG0tjj7wqiJBiAfCtKK2KoMzIB/NY+pwZvPykUlQU04=;
12, 53 -- b=OwKSy+dcN9npjYB3vKs09+W4daGXOunq9SBaEmoU3L9vNrPHIc2BNF5xG3mzdODORP
12, 53 -- bzptunlcBdaAxoZU5nwHio+8YJYc9rBCH7+rg+KddZ4MJDLqLeiAPUPAaQo47xJsKXr/
12, 53 -- Mt39hImMOj3Z9++sHhCcBDZU/H8oWSHucvBqiyXgahlTM3sEmKlJKvRMklAlvbU1WL3T
12, 53 -- QNjmuA0xd5e3RsDyk0RAjqCYPVxVLSx8X11f10drzIx/7VVyeabC5zMX1gAlWoAfFMIM
12, 53 -- BmCcNgLjuVKnpCrtdzR+nYoa/vfQ4ZbRnpT7dESSoorTctbBkgjRgScjYAFyI/1tg578
12, 53 -- aHsw==
12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 53 -- d=1e100.net; s=20130820;
12, 53 -- h=x-gm-message-state:from:subject:references:reply-to:to:message-id
12, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 53 -- :content-transfer-encoding;
12, 53 -- bh=nG0tjj7wqiJBiAfCtKK2KoMzIB/NY+pwZvPykUlQU04=;
12, 53 -- b=anmKNpm7KDX037RxPq7U851ZM71rGqhpRv/J7LQ/ZRO5LEXYr6vK6ga+1pcy/07QD5
12, 53 -- hhdooNsxvq2uZkbW9mg+GcTsaUWHO9/s4z9Ph0ZbxX8HomN2vT95HZ/o+sH2lpOPt3xC
12, 53 -- 80yFpAHPW7Oiad+rExMFbR62p10MbLrJEGt90w4U9gUlyreK5UOwlQnFwfv0eHGEtk81
12, 53 -- M7c5MzPUdQhli/T9a/CgECEA1RcjHVWFeDd+vH6tMoF1w8lnpKRd7ViAKrbOiNpgrvZq
12, 53 -- V1qmlsf1HUob62ucSAm7D0FJEewrS+0A/zBqgfiuwMn5PZtbIstBCvLOBR8hB64ibldO
12, 53 -- 8HQw==
12, 53 -- X-Gm-Message-State: AG10YOQXeMUEFHYkyxI4cepuo9UvyNuBYtTDGzsDmmuYLbpramSKdqaVJGNx7C3KrPimdA==
12, 53 -- X-Received: by 10.50.43.131 with SMTP id w3mr3000983igl.70.1452946308661;
12, 53 -- Sat, 16 Jan 2016 04:11:48 -0800 (PST)
12, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
12, 53 -- by smtp.googlemail.com with ESMTPSA id 124sm5742277ioz.10.2016.01.16.04.11.48
12, 53 -- for {microscopy-at-microscopy.com}
12, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 53 -- Sat, 16 Jan 2016 04:11:48 -0800 (PST)
12, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
12, 53 -- {microscopylistserver-noreply-at-microscopy.com}
12, 53 -- Subject: viaWWW:ehigh Microscopy School 2016
12, 53 -- References: {201601152100.u0FL0UM6031469-at-ns.microscopy.com}
12, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 53 -- X-Forwarded-Message-Id: {201601152100.u0FL0UM6031469-at-ns.microscopy.com}
12, 53 -- Message-ID: {569A3383.1030505-at-microscopy.com}
12, 53 -- Date: Sat, 16 Jan 2016 06:11:47 -0600
12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
12, 53 -- Gecko/20100101 Thunderbird/38.5.1
12, 53 -- MIME-Version: 1.0
12, 53 -- In-Reply-To: {201601152100.u0FL0UM6031469-at-ns.microscopy.com}
12, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed
12, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================






From: microscopy.listserver-at-gmail.com
Date: Mon, 18 Jan 2016 19:17:46 -0600
Subject: [Microscopy] viaWWW:Microanalysis Society Topical Conference,Electron-Probe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: paul.carpenter.epma-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both paul.carpenter.epma-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: paul.carpenter.epma-at-gmail.com
Name: Paul Carpenter

Organization: MAS

Title-Subject: [Filtered] MAS Electron-Probe Microanalysis 2016 workshop

Message: Microanalysis Society Topical Conference
Electron-Probe Microanalysis 2016

May 16-19, 2016 at the University of Wisconsin-Madison, WI

http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016



Registration is now open
See website for full details
Abstract deadline February 15, 2016
MAS Early Career Scholar financial travel support

Four-day intensive MAS topical conference

User meetings with EPMA 2016 sponsors on Monday, May 16, 2016
Three-day intensive topical conference, Tues-Thurs May 17-19, 2016
- EPMA, SEM, WDS, EDS techniques

Tutorial on EPMA and SEM quantitative analysis

Topics with invited and contributed presentations

General electron-probe microanalysis: analysis, instrumentation, methods

Trace element EPMA: background methods, high-resolution EPMA

Advanced WDS and EDS techniques, Monte Carlo simulation, accuracy studies

Quantitative compositional mapping by EPMA

Cathodoluminescence

Presentations and product information from the microanalysis vendor community

Inexpensive housing options

Group meals and discussion

Problems identified by the microanalysis community
-- solutions provided by the vendor community


Contact Paul Carpenter for more information: paul.carpenter.epma-at-gmail.com


Paul Carpenter
Earth and Planetary Sciences CB1169
Washington University
1 Brookings Drive
Saint Louis, MO 63130
314-602-9697






--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
36, 54 -- From microscopy.listserver-at-gmail.com Mon Jan 18 19:17:43 2016
36, 54 -- Received: from mail-ig0-f178.google.com (mail-ig0-f178.google.com [209.85.213.178])
36, 54 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0J1Hhr9029746
36, 54 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jan 2016 19:17:43 -0600
36, 54 -- Received: by mail-ig0-f178.google.com with SMTP id z14so66968541igp.1
36, 54 -- for {microscopy-at-microscopy.com} ; Mon, 18 Jan 2016 17:17:32 -0800 (PST)
36, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
36, 54 -- d=gmail.com; s=20120113;
36, 54 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
36, 54 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
36, 54 -- bh=9Krt8xtquKjmn3kkXT7klVAFbMxV/Ed5VBsF8ehRRzw=;
36, 54 -- b=u6z5gO3yvql5RzvIN0mRh17XLamPkVDi5v9JKdL0by2bRCNsD/PoMMh8Ms50VsFcv/
36, 54 -- 3cRIxTBQHZSyJxQfx6zq2eyxsm0ZC5go42H1XGbTsXNuPld8oo6itUha9W36O0wbovnu
36, 54 -- 2UuxnwvyRdyJGLoDVubrxFG4fSR1xweTpT5EU7iCE6dsgBpxezU7J8GS0VDzs4WJLBNG
36, 54 -- mcLxxv4nJkL7Cdxfx9ZZI69GGBNMAmwKL1YZI7z0kDwx/haZUwmoWoAnJfJGVcSe0Qsz
36, 54 -- 92WIv+X7sBLAGH7/VNe1Blp6ECf5oJcNXoGlsYE/6Dg2TvHvlrPJrg5iqPhMiQik5oAZ
36, 54 -- qg+g==
36, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
36, 54 -- d=1e100.net; s=20130820;
36, 54 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
36, 54 -- :date:user-agent:mime-version:in-reply-to:content-type
36, 54 -- :content-transfer-encoding;
36, 54 -- bh=9Krt8xtquKjmn3kkXT7klVAFbMxV/Ed5VBsF8ehRRzw=;
36, 54 -- b=cmXTJFDWTW+0LoG2qUyRM7OTKi3JaaiTMMNNy/IdpIgb/sU78xfWjKZltvXt/RXjCt
36, 54 -- 0c5hqIjoTFcc7IQo9N9VdRE0M+bJMR+Us322xEFuzwEK0EgvCt9ZypJWQT7vftGdyI3w
36, 54 -- xHror4AAOghmXRZZUh4lf/cAkrRcavM9AhFYkgjB06UkCIvlls3oaHMh16XNX+hcV9xF
36, 54 -- /6n/LE1ssDhA2iKHobPBfmK4kWGtx3RSPLhjSjNYedR6diujSUY5Mov98wtQIi0tgGNg
36, 54 -- L/dRDeMwaEn7/o9JtSYB81txZ5l1NzOHj6ZbLw4Rxw7pSY+vTMbsJYbyfqxMXo9pMuXD
36, 54 -- 8nKg==
36, 54 -- X-Gm-Message-State: AG10YOTZEBCAgkv02fHspMsuf6KwtkfMZ0AwPR2ZLcmP40Pgq+dnxc6n03kGnUJV+iI5PA==
36, 54 -- X-Received: by 10.50.79.231 with SMTP id m7mr13499352igx.15.1453166252092;
36, 54 -- Mon, 18 Jan 2016 17:17:32 -0800 (PST)
36, 54 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
36, 54 -- by smtp.googlemail.com with ESMTPSA id z14sm2474205igg.11.2016.01.18.17.17.31
36, 54 -- for {microscopy-at-microscopy.com}
36, 54 -- (version=TLSv1/SSLv3 cipher=OTHER);
36, 54 -- Mon, 18 Jan 2016 17:17:31 -0800 (PST)
36, 54 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
36, 54 -- X-Google-Original-From: MicroscopyListserver-NoReply
36, 54 -- {microscopylistserver-noreply-at-microscopy.com}
36, 54 -- Subject: viaWWW:Microanalysis Society Topical Conference,Electron-Probe
36, 54 -- Microanalysis 2016
36, 54 -- References: {201601190112.u0J1CoqH029690-at-ns.microscopy.com}
36, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
36, 54 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
36, 54 -- X-Forwarded-Message-Id: {201601190112.u0J1CoqH029690-at-ns.microscopy.com}
36, 54 -- Message-ID: {569D8EAA.4070008-at-microscopy.com}
36, 54 -- Date: Mon, 18 Jan 2016 19:17:30 -0600
36, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
36, 54 -- Gecko/20100101 Thunderbird/38.5.1
36, 54 -- MIME-Version: 1.0
36, 54 -- In-Reply-To: {201601190112.u0J1CoqH029690-at-ns.microscopy.com}
36, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed
36, 54 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Jan 2016 06:35:52 -0600
Subject: [Microscopy] viaWWW:Looking for EDS for RJ Lee PSEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: mk-at-seclab.tuwien.ac.at

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mk-at-seclab.tuwien.ac.at as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: mk-at-seclab.tuwien.ac.at
Name: Markus Kammerstetter

Organization: Secure Systems Lab Vienna, Vienna University of Technology

Title-Subject: [Filtered] Looking for EDS for RJ Lee PSEM

Message: Hello,

in our lab we have an older model RJ Lee/Aspex PSEM Scanning Electron Microscope in use to image and
analyze microchips.
We deprocess those chips layer by layer but currently do not have the exact information of the
material compositions used in those layers. Since the deprocessing and material removal techniques
(i.e. wet etching) heavily depend on the materials, we have been looking for an EDS system for quite
a while but unfortunately do not really have a budget for it.

It would thus be great if anyone on this list could spare a surplus EDS that is no longer needed
(detector, control HW and possibly also the SW tool).
Ideally it would include light elements and be compatible with the Aspex/RJ Lee PSEM so that we
could use the PSEM's software. However, virtually any EDS would also be nice to have if we can make
it fit. Also just heavy elements are fine as this would still allow us to characterize Si, Cu, Al,
W, Au and other material compositions typically found in integrated circuits.

We have access to LN2 for cooling, we can typically do mechanical and electronics repairs on our own
and we could also mill a custom adapter to make it fit onto our PSEM port.


Thank you,
Markus

Login Host: 80.108.8.29
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
17, 53 -- From microscopy.listserver-at-gmail.com Tue Jan 19 06:35:49 2016
17, 53 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171])
17, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0JCZmV5031072
17, 53 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jan 2016 06:35:48 -0600
17, 53 -- Received: by mail-ig0-f171.google.com with SMTP id t15so76224241igr.0
17, 53 -- for {microscopy-at-microscopy.com} ; Tue, 19 Jan 2016 04:35:37 -0800 (PST)
17, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 53 -- d=gmail.com; s=20120113;
17, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
17, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
17, 53 -- bh=pHbRwGW5HDThoQO8owOLSZTlY6EOmoORVqczVNG9E3k=;
17, 53 -- b=HUsbmzuDvuErhoKTxj/SCRRjPdlLQ6Wb8oGMOvQJlKlJQ/jAlxz8vxIyQfqZ6vRt1B
17, 53 -- f8MK124u0y70z3uskvQSJiQG8085pdjyhkY2RNBHK4K5QZvqpdker0OAHQwUS7pCjgdl
17, 53 -- ockgiraENupfMafNEi7pkznklBLR5YSK3tFfNQ2y/WF4QMZIkGrj0AyhZOf4cvINhoBE
17, 53 -- fR9ePgzVR4WFH2RVlLMy2U6d9fczO+19D7QruMkmYpmQtcbHM28sduoWmv23AwIlq1PV
17, 53 -- aNtdWx+tPmdRVigrjkBmk0rz1zW876gV8/89WaavRKtLtjL5m1x9kxRET6x+PCNjuS7s
17, 53 -- eiZQ==
17, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 53 -- d=1e100.net; s=20130820;
17, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
17, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
17, 53 -- :content-transfer-encoding;
17, 53 -- bh=pHbRwGW5HDThoQO8owOLSZTlY6EOmoORVqczVNG9E3k=;
17, 53 -- b=KPbqz+7Aesk04gS33Khpew49TaTdtHKV31T4CFMEBCqUn8mrQk2d584u/tBDB5B+1/
17, 53 -- rFrK8tCqHTkFRzXp1D9mDWxPxpFj/3noHwI+/maDq+Plx+/le6qBq0TQIvdWMZteZvf/
17, 53 -- Is9ffA1kKE3t9CTLJf3uUP5kiP6dGg105FRGFJSNJOr+YODWUKERlyDScZQtXyO2aUO/
17, 53 -- 0ww/Uwkj3FpG4dzmF1t7M/UvPj8CKZKHA/Aoa+PbahSOt4TaqkUdO6MggcWl/Dw9MZpV
17, 53 -- tRk6rbsytiDo4A4J6oS23CwmMxBYVM5QzL3ZEIzStfh1N1OC6jTyd66+ajXICoXMwalQ
17, 53 -- VCNw==
17, 53 -- X-Gm-Message-State: AG10YOSR/HrI707SdWEWYFw9MDewOTEwqylwT5YBEjPOAVb9/oPwp0oo+BmJgn5zz4qKNw==
17, 53 -- X-Received: by 10.50.50.228 with SMTP id f4mr15649800igo.6.1453206937453;
17, 53 -- Tue, 19 Jan 2016 04:35:37 -0800 (PST)
17, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
17, 53 -- by smtp.googlemail.com with ESMTPSA id r80sm10562024ioi.26.2016.01.19.04.35.36
17, 53 -- for {microscopy-at-microscopy.com}
17, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 53 -- Tue, 19 Jan 2016 04:35:36 -0800 (PST)
17, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
17, 53 -- {microscopylistserver-noreply-at-microscopy.com}
17, 53 -- Subject: viaWWW:Looking for EDS for RJ Lee PSEM
17, 53 -- References: {201601190831.u0J8VvEq024604-at-ns.microscopy.com}
17, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
17, 53 -- X-Forwarded-Message-Id: {201601190831.u0J8VvEq024604-at-ns.microscopy.com}
17, 53 -- Message-ID: {569E2D98.1020203-at-microscopy.com}
17, 53 -- Date: Tue, 19 Jan 2016 06:35:36 -0600
17, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 53 -- Gecko/20100101 Thunderbird/38.5.1
17, 53 -- MIME-Version: 1.0
17, 53 -- In-Reply-To: {201601190831.u0J8VvEq024604-at-ns.microscopy.com}
17, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
17, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Jan 2016 06:09:46 -0600
Subject: [Microscopy] viaWWW: Inviting you to attend and participate in EBSD 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: lnbrewer1-at-eng.ua.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both lnbrewer1-at-eng.ua.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: lnbrewer1-at-eng.ua.edu
Name: Luke Brewer

Organization: University of Alabama

Title-Subject: [Filtered] Inviting you to attend and participate in EBSD 2016

Message: Dear Colleagues,

I invite you to attend and participate in Electron Backscatter Diffraction 2016! EBSD 2016 will be
held at The University of Alabama from May 24-26, 2016.

EBSD 2016 will have a one-day tutorial session with both lectures and hands-on laboratory
demonstrations designed to introduce EBSD to students and professionals new to the technique. There
will also be more advanced tutorials for experienced users on topics such as precession electron
diffraction, high angular resolution EBSD, and transmission kikuchi diffraction.

Days 2-3 will have platform presentations and posters devoted to the latest in EBSD-technique
development and applications to materials science, geology and earth sciences, and materials
engineering.

Support will be available to encourage students to attend this meeting.

Registration is now open, and we are accepting abstracts on-line until March 1, 2016.

Full details on the meeting can be found at:
http://www.microbeamanalysis.org/topical-conferences/ebsd-2016/welcome

Please let me know if you have any questions, and we do hope to see you at EBSD 2016!

Luke Brewer

Login Host: 130.160.6.60
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
20, 53 -- From microscopy.listserver-at-gmail.com Wed Jan 20 06:09:42 2016
20, 53 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182])
20, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0KC9fUv017743
20, 53 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jan 2016 06:09:41 -0600
20, 53 -- Received: by mail-io0-f182.google.com with SMTP id q21so17407209iod.0
20, 53 -- for {microscopy-at-microscopy.com} ; Wed, 20 Jan 2016 04:09:30 -0800 (PST)
20, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 53 -- d=gmail.com; s=20120113;
20, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
20, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
20, 53 -- bh=PvhzLpPwDSj8awYKWd8MUCICl5FpID13p/MZ5Oz/8WY=;
20, 53 -- b=hjFLAuZHt/H7XA/8qSHAJlpIdXF+JTJ+6cTJu+cI7XEXFausPth3ATvJV70YZzjELG
20, 53 -- BVKnxi3ZfZV98EYvNfegsrOZzjaggTaWgeO6P1dLsAMW+PRQNwDaqOjtPFp+o3YPlJPw
20, 53 -- HCBuXutpMMvckqFPFakx6JUqnrzfeJv1FMwwJUXxer7lVVebcytty09pbvn1ACx+2Bez
20, 53 -- nvobLryCXQ+pVvB1B2oVs0Y3NKKmnRYmy951BuT5Ou3jNBaczeyPkBONZLf41VqAXYdL
20, 53 -- gZ0DtODYD7swqGBzChTp9PPKH1ebbG+tcweROiFtD/lef7P2R7LwfWPmmNQ9NHKGc1TK
20, 53 -- WrWQ==
20, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 53 -- d=1e100.net; s=20130820;
20, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
20, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
20, 53 -- :content-transfer-encoding;
20, 53 -- bh=PvhzLpPwDSj8awYKWd8MUCICl5FpID13p/MZ5Oz/8WY=;
20, 53 -- b=j4crpENVPdcDw8+dlqIcYctsB1SP2DqYmcnf9MfFB0/SSMTryNoUjLMP6mOHCevnki
20, 53 -- uJUZ4jFckP4q9AlM5VPVMMi8xgpR/xBhuzVIrVUrPg5b/QxOSQeN0dVftIQQnlLOvl2r
20, 53 -- QVwJHXjuj3/0l3uADPY0b5xYHLcbJXAlm+xvNkG6Se7pCTWTuKPZ7Hpm8e83+ZidDv86
20, 53 -- Osw7J10YvID/y73NzNLiHFRcUMYZkylWVGVb3zwrzQER9cVNa+tpHJMODB4SM0hn7SLP
20, 53 -- igRwUY+3ZfSfR45UMedtDZ7ydVYyNW76tmU1aGK53v//jGx1eC/4ExHPtxJdaTFfPOmp
20, 53 -- VH1A==
20, 53 -- X-Gm-Message-State: AG10YOSiGYNQm7zIRJU1p9rc/lF2HLxUKt2y1cpnRGBaEimiR03gLM/YxPCK3IEL0P7QcQ==
20, 53 -- X-Received: by 10.107.40.148 with SMTP id o142mr2202414ioo.18.1453291770085;
20, 53 -- Wed, 20 Jan 2016 04:09:30 -0800 (PST)
20, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
20, 53 -- by smtp.googlemail.com with ESMTPSA id yq10sm8913160igb.8.2016.01.20.04.09.29
20, 53 -- for {microscopy-at-microscopy.com}
20, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
20, 53 -- Wed, 20 Jan 2016 04:09:29 -0800 (PST)
20, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
20, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
20, 53 -- {microscopylistserver-noreply-at-microscopy.com}
20, 53 -- Subject: viaWWW: Inviting you to attend and participate in EBSD 2016
20, 53 -- References: {201601191725.u0JHPk5e027443-at-ns.microscopy.com}
20, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
20, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
20, 53 -- X-Forwarded-Message-Id: {201601191725.u0JHPk5e027443-at-ns.microscopy.com}
20, 53 -- Message-ID: {569F78F9.4090800-at-microscopy.com}
20, 53 -- Date: Wed, 20 Jan 2016 06:09:29 -0600
20, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
20, 53 -- Gecko/20100101 Thunderbird/38.5.1
20, 53 -- MIME-Version: 1.0
20, 53 -- In-Reply-To: {201601191725.u0JHPk5e027443-at-ns.microscopy.com}
20, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
20, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: john.robson-at-boehringer-ingelheim.com
Date: Wed, 20 Jan 2016 10:57:49 -0600
Subject: [Microscopy] Pre-cleaned Microscope Slide recommendation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am having problems finding pre-cleaned microscope slides that are actually clean. I've looked at recently purchased slides form 2 vendors but neither product is "clean" with easily visible particulates and splotchy thin films randomly covering slide surfaces. Is anyone else out there noticing that previously good sources are now "dirty". I am using Polarized and bright field illumination. I would appreciate a product recommendation if you currently have a high quality source.

Thanks In advance, John


==============================Original Headers==============================
3, 38 -- From prvs=82076f2d2=john.robson-at-boehringer-ingelheim.com Wed Jan 20 10:57:45 2016
3, 38 -- Received: from mail4.boehringer-ingelheim.com (mail4.boehringer-ingelheim.com [148.188.192.93])
3, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0KGviGd013813
3, 38 -- for {Microscopy-at-microscopy.com} ; Wed, 20 Jan 2016 10:57:44 -0600
3, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=simple/simple;
3, 38 -- d=boehringer-ingelheim.com; i=-at-boehringer-ingelheim.com;
3, 38 -- q=dns/txt; s=boehringer; t=1453309053; x=1484845053;
3, 38 -- h=from:to:subject:date:message-id:
3, 38 -- content-transfer-encoding:mime-version;
3, 38 -- bh=MvNlyaHtvzhrtXU44DoiKpxb+qe1b1G1gNQEjkBoMAE=;
3, 38 -- b=m/HIEjolcQXYpaZ8PeVS6rehKsvlQMHJm90RCp+lolsAG0TNbj93OnNM
3, 38 -- qbsVhq83+DHd+DW2priyeX1rHlqvtRyts2lKm4CL/m/0K6chIMQKwG/0d
3, 38 -- rtCjMzb4myQqZ5hJShgjcrL2EhkuGBlRzOgSxYGhMRBa3Lf0CcrUsIOLD
3, 38 -- c=;
3, 38 -- X-IronPort-AV: E=McAfee;i="5700,7163,8049"; a="157517773"
3, 38 -- X-IronPort-AV: E=Sophos;i="5.22,321,1449529200";
3, 38 -- d="scan'208";a="157517773"
3, 38 -- Received: from unknown (HELO NAHEXCH04.am.boehringer.com) ([10.128.102.229])
3, 38 -- by mail4.boehringer-ingelheim.com with ESMTP/TLS/AES128-SHA; 20 Jan 2016 17:57:32 +0100
3, 38 -- Received: from NAHEXMB05.am.boehringer.com ([fe80::741e:f747:6a63:ba83]) by
3, 38 -- NAHEXCH04.am.boehringer.com ([fe80::b496:454c:6124:4100%19]) with mapi id
3, 38 -- 14.03.0224.002; Wed, 20 Jan 2016 11:57:32 -0500
3, 38 -- From: {john.robson-at-boehringer-ingelheim.com}
3, 38 -- To: {Microscopy-at-microscopy.com}
3, 38 -- Subject: Pre-cleaned Microscope Slide recommendation
3, 38 -- Thread-Topic: Pre-cleaned Microscope Slide recommendation
3, 38 -- Thread-Index: AdFTo6bEICL8CSKCSlybB1gtBsEouw==
3, 38 -- Date: Wed, 20 Jan 2016 16:57:32 +0000
3, 38 -- Message-ID: {0505FBCD63EE71428E13EBEEFC2C4E43281C45CC-at-NAHEXMB05.am.boehringer.com}
3, 38 -- Accept-Language: en-US
3, 38 -- Content-Language: en-US
3, 38 -- X-MS-Has-Attach:
3, 38 -- X-MS-TNEF-Correlator:
3, 38 -- x-originating-ip: [10.128.230.250]
3, 38 -- Content-Type: text/plain; charset="us-ascii"
3, 38 -- MIME-Version: 1.0
3, 38 -- Content-Transfer-Encoding: 8bit
3, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0KGviGd013813
==============================End of - Headers==============================






From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Jan 2016 06:55:59 -0600
Subject: [Microscopy] viaWWW:Seeking a repairperson for a Sorvall MT7000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Katherine.Kocan-at-okstate.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both Katherine.Kocan-at-okstate.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: Katherine.Kocan-at-okstate.edu
Name: Katherine Kocan

Organization: OSU Center for Veterinary Health Sciences

Title-Subject: [Filtered] Seeking a repairperson for a Sorvall MT7000

Message: If anyone has the name and contact information for a person who could provide service for a
Sorvall MT-7000, please share this information with me.

Thanks very much.

Kathy Kocan

Login Host: 139.78.210.162
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 53 -- From microscopy.listserver-at-gmail.com Thu Jan 21 06:55:55 2016
14, 53 -- Received: from mail-ig0-f177.google.com (mail-ig0-f177.google.com [209.85.213.177])
14, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0LCtspS025392
14, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jan 2016 06:55:54 -0600
14, 53 -- Received: by mail-ig0-f177.google.com with SMTP id mw1so32791247igb.1
14, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jan 2016 04:55:43 -0800 (PST)
14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 53 -- d=gmail.com; s=20120113;
14, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
14, 53 -- bh=gOCKkBYad8as/i8ERDaYAtX8Sb/yjDFjWDWIaTkyVPs=;
14, 53 -- b=ykAquvskUYQsa3f4L0YhLIaaPMjRRw+S6tMSMtqS1OK115Khx/FEbpJDT8Kq9GeSbT
14, 53 -- aZaHaihCGwFc3l1fTxox6Qj4mPZ3aSs2R2rMLO8uz9rjeOieWk5cnJ3J9OsGSUViE/eE
14, 53 -- SlUl/81+cTqkOx18aez3QEoFP8XA67wQzL0PTAnTqaCxLOsaVTHQ4xKldrRNqKOScYhT
14, 53 -- Rv45UUiMnkuPMMSOPnKXNa40xDFIgio/Y07tyqYKkEgUB/cBo1OFPm9gGjU0V1QutJIN
14, 53 -- gCXHhQ0Ib87XD6MO9Mh4K/ZnbcB/evc0C65wSYHKulyAlGN5GJgdYrd/r3t/tSOuVYIK
14, 53 -- r2ig==
14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 53 -- d=1e100.net; s=20130820;
14, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
14, 53 -- :content-transfer-encoding;
14, 53 -- bh=gOCKkBYad8as/i8ERDaYAtX8Sb/yjDFjWDWIaTkyVPs=;
14, 53 -- b=W/iv6tzsGXObqo+E+URtooeg4MN23ubBBLMDlPEHLjZeaD84/Gdc1tfx5P9vpyjkL+
14, 53 -- r1S5TVDbQtfh89ojX2J5mrhf83BJ2/v1mpRa4I6lMD6a1A701fddeAOiol7zDVulwNzi
14, 53 -- Chwwv1WTfUg5IJj14kWBBslv/Dx3IRAZdOc/ZU5+/GYXRSfGiKr6FatWK28YVknr6QHW
14, 53 -- BuyIGZtNpOwH2P/1/mBZ/gHtZRjN+vc1LpGIy4IgznCm+yEAlNpK8rSgO45z1Dn8vO5+
14, 53 -- DhKmGctVKyINKzWlj3+rP2T4brrYtqxUZQ6XUYg7rYyU1p30N/+S4t0nWRmzM62bXYte
14, 53 -- BqyA==
14, 53 -- X-Gm-Message-State: AG10YORvr9jbk7q+xmJ7c8VWZviVZZ5oFYuHSLy5rPQFS8lgl2+Yv1QDJ2lp2OCfjMpc1g==
14, 53 -- X-Received: by 10.50.124.72 with SMTP id mg8mr8823095igb.22.1453380942875;
14, 53 -- Thu, 21 Jan 2016 04:55:42 -0800 (PST)
14, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 53 -- by smtp.googlemail.com with ESMTPSA id ki7sm1077025igb.2.2016.01.21.04.55.41
14, 53 -- for {microscopy-at-microscopy.com}
14, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 53 -- Thu, 21 Jan 2016 04:55:42 -0800 (PST)
14, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 53 -- {microscopylistserver-noreply-at-microscopy.com}
14, 53 -- Subject: viaWWW:Seeking a repairperson for a Sorvall MT7000
14, 53 -- References: {201601201721.u0KHLRCq017228-at-ns.microscopy.com}
14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 53 -- X-Forwarded-Message-Id: {201601201721.u0KHLRCq017228-at-ns.microscopy.com}
14, 53 -- Message-ID: {56A0D54D.3020900-at-microscopy.com}
14, 53 -- Date: Thu, 21 Jan 2016 06:55:41 -0600
14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
14, 53 -- Gecko/20100101 Thunderbird/38.5.1
14, 53 -- MIME-Version: 1.0
14, 53 -- In-Reply-To: {201601201721.u0KHLRCq017228-at-ns.microscopy.com}
14, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Jan 2016 18:44:16 -0600
Subject: [Microscopy] viaWWW:EDS detector window problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: wzhe-at-laurentian.ca

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wzhe-at-laurentian.ca as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: wzhe-at-laurentian.ca
Name: William

Organization: Laurentian University

Title-Subject: [Filtered] EDS detector window problem

Message: Hello,

We currently had problem with EDS SDD detector , it couldn-it be cooling down and a -eFault-i message
for detector condition came out at the end. One recommendation is that is sign of detector window
blown or pin hole on it. Is somebody ever went through this type of issue and have ideas about

1. how to identify if it is a window problem?
2. is it a simple job to replace a defective window if you have parts, like people always do for WDS
detector on their own ? If not,
3. any service company offers repairing in north America you may recommend ?

Thanks so much in advance,

William


Login Host: 142.51.53.73
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
16, 53 -- From microscopy.listserver-at-gmail.com Thu Jan 21 18:44:14 2016
16, 53 -- Received: from mail-qk0-f171.google.com (mail-qk0-f171.google.com [209.85.220.171])
16, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0M0iBSr025588
16, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jan 2016 18:44:12 -0600
16, 53 -- Received: by mail-qk0-f171.google.com with SMTP id x1so23221071qkc.1
16, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 Jan 2016 16:44:01 -0800 (PST)
16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 53 -- d=gmail.com; s=20120113;
16, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
16, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
16, 53 -- bh=l0CYwwrW5rMrJf3nj4U1C1eTGuUWS3MjkFtLo44kyWQ=;
16, 53 -- b=ODAhOpIr/vOVYNl/l3epQ7itQ63BNov+5InLoUp9R5zcuh5L3xHrSme83yD5uN7IBF
16, 53 -- cnYfFfnhkIVdSPGC4a1OI6a0apF/o1KzfFK0r6ikjKqEkXoa54TzDQRAwrKDe+fdGiK8
16, 53 -- 6jVLJOKQOyVo5v7DUMQpcKks7oCXRIaoBFR3uvz8rsdZ357oLb+7tPIoE4p4o0SLNzp+
16, 53 -- YTj91WiI+1G3/A7eJXtnWqClnK26Ln122xKsXY4u9Fziu0buvvySS00UPliHJHURKaQG
16, 53 -- HNweHjekJUpWPcY2HgzxVklxPLbdibn95O47jaaPY4bsRd+Zy5MmOeBv2TETBbgU9BRR
16, 53 -- llBg==
16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 53 -- d=1e100.net; s=20130820;
16, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
16, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
16, 53 -- :content-transfer-encoding;
16, 53 -- bh=l0CYwwrW5rMrJf3nj4U1C1eTGuUWS3MjkFtLo44kyWQ=;
16, 53 -- b=YTFuUtZPqhSP0JLr7C/yQIIlNM/zkJzX6La00CxhUxDty4JuuD53GDp4/6QhwtCq0G
16, 53 -- 9icNiuL8+hVsJRESca1YW9KhfNXZm4cT3pJ3XoxwJfjyEXGkpPCXZsU8x8y2Fdyxdrds
16, 53 -- kIHje0PdzDUekvpjnuo6p33//TZ1lkFgKfMfDchhdvJv44tAlT8yVD8LlfOXVZo8U81v
16, 53 -- FdofyYLAErPb4I8spfiAcPX45Jvv1Mfytm8i0UDFQBT1+D4v2M956BzhKzG9dsC/WRlr
16, 53 -- fZuMvSKcOs6NnCgfQQrDnWneTivQMGG9khIME0IBqYYZZcnd+skQlfel2wXLN2PowpuA
16, 53 -- 9kxw==
16, 53 -- X-Gm-Message-State: AG10YOSU5Cbjj1Rt3jT6f5dEQ06EAuxYNEI19AT3r1ACYBcb1KDiKzs40MfRifqKbASmcA==
16, 53 -- X-Received: by 10.55.215.91 with SMTP id m88mr325615qki.8.1453423440177;
16, 53 -- Thu, 21 Jan 2016 16:44:00 -0800 (PST)
16, 53 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1])
16, 53 -- by smtp.googlemail.com with ESMTPSA id o78sm1702705qko.24.2016.01.21.16.43.59
16, 53 -- for {microscopy-at-microscopy.com}
16, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 53 -- Thu, 21 Jan 2016 16:43:59 -0800 (PST)
16, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
16, 53 -- {microscopylistserver-noreply-at-microscopy.com}
16, 53 -- Subject: viaWWW:EDS detector window problem
16, 53 -- References: {201601211929.u0LJT8Jp020734-at-ns.microscopy.com}
16, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
16, 53 -- X-Forwarded-Message-Id: {201601211929.u0LJT8Jp020734-at-ns.microscopy.com}
16, 53 -- Message-ID: {56A17B4E.1050306-at-microscopy.com}
16, 53 -- Date: Thu, 21 Jan 2016 18:43:58 -0600
16, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
16, 53 -- Gecko/20100101 Thunderbird/38.5.1
16, 53 -- MIME-Version: 1.0
16, 53 -- In-Reply-To: {201601211929.u0LJT8Jp020734-at-ns.microscopy.com}
16, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed
16, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: wsalmon-at-wi.mit.edu
Date: Sat, 23 Jan 2016 12:33:49 -0600
Subject: [Microscopy] LM - 2016 Analytical and Quantitative Light Microscopy Course @ MBL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Application Deadline Monday, January 25. Please spread the word.

Dear Colleagues,

We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run May 4 - May 13 , 2016.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.

Laboratory exercises, demonstrations, and discussions include:
* geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
* phase contrast, polarization and interference microscopy;
* fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
* principles and application of digital imaging and quantitative digital image deconvolution;
* digital image processing and object identification and tracking;
* live cell and ratiometric imaging for FRET and ion concentration imaging;
* confocal microscopy and specialized methods such as TIRF and FLIM; and
* new advances in light microscopy such as FCS, PALM, STORM, SIM and SPIM.

The course web site is at http://www.mbl.edu/aqlm .

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Applications due January 25 , 2016.

Directors: Jagesh Shah (Harvard Medical School), Justin Taraska (National Institutes of Health)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Cheers,
Jagesh, Justin and Wendy

Posters available at http://www.mbl.edu/education/files/2014/04/AQLM_POSTER_2016v1.pdf

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
9 Cambridge Center, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/

==============================Original Headers==============================
13, 40 -- From wsalmon-at-wi.mit.edu Sat Jan 23 12:33:24 2016
13, 40 -- Received: from ptolemy.wi.mit.edu (ptolemy.wi.mit.edu [18.4.1.120])
13, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0NIXIGM016281
13, 40 -- for {microscopy-at-microscopy.com} ; Sat, 23 Jan 2016 12:33:19 -0600
13, 40 -- X-IronPort-AV: E=Sophos;i="5.22,333,1449550800";
13, 40 -- d="scan'208";a="3640678"
13, 40 -- Received: from unknown (HELO mars.wi.mit.edu) ([10.9.4.20])
13, 40 -- by ptolemy.wi.mit.edu with ESMTP; 22 Jan 2016 16:11:38 -0500
13, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1])
13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id B7D2631E138
13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:38 -0500 (EST)
13, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1])
13, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10032)
13, 40 -- with ESMTP id TZ2FtvMjteGg for {microscopy-at-microscopy.com} ;
13, 40 -- Fri, 22 Jan 2016 16:11:36 -0500 (EST)
13, 40 -- Received: from localhost (localhost.localdomain [127.0.0.1])
13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 931EE31E13E
13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:36 -0500 (EST)
13, 40 -- X-Virus-Scanned: amavisd-new at mars.wi.mit.edu
13, 40 -- Received: from mars.wi.mit.edu ([127.0.0.1])
13, 40 -- by localhost (mars.wi.mit.edu [127.0.0.1]) (amavisd-new, port 10026)
13, 40 -- with ESMTP id MJ_iGgBykbZT for {microscopy-at-microscopy.com} ;
13, 40 -- Fri, 22 Jan 2016 16:11:36 -0500 (EST)
13, 40 -- Received: from io.wi.mit.edu (io.wi.mit.edu [10.9.4.21])
13, 40 -- by mars.wi.mit.edu (Postfix) with ESMTP id 77FD131E138
13, 40 -- for {microscopy-at-microscopy.com} ; Fri, 22 Jan 2016 16:11:36 -0500 (EST)
13, 40 -- Date: Fri, 22 Jan 2016 16:11:36 -0500 (EST)
13, 40 -- From: Wendy Salmon {wsalmon-at-wi.mit.edu}
13, 40 -- To: microscopy {microscopy-at-microscopy.com}
13, 40 -- Message-ID: {344470042.11777.1453497096429.JavaMail.zimbra-at-wi.mit.edu}
13, 40 -- In-Reply-To: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu}
13, 40 -- References: {816105877.10462683.1452116518458.JavaMail.zimbra-at-wi.mit.edu}
13, 40 -- Subject: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL
13, 40 -- MIME-Version: 1.0
13, 40 -- Content-Type: text/plain; charset=utf-8
13, 40 -- Content-Transfer-Encoding: 7bit
13, 40 -- X-Originating-IP: [134.174.140.59]
13, 40 -- X-Mailer: Zimbra 8.6.0_GA_1191 (ZimbraWebClient - GC47 (Mac)/8.6.0_GA_1191)
13, 40 -- Thread-Topic: LM - 2016 Analytical and Quantitative Light Microscopy Course -at- MBL
13, 40 -- Thread-Index: 3I4RFXAc6NuHKgHiTFr8kP+ljEXmnAHyDNBp
==============================End of - Headers==============================




From: marksmsa-at-gmail.com
Date: Mon, 25 Jan 2016 07:56:49 -0600
Subject: [Microscopy]

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I would like to draw your attention to the call for nominations for
the Gjonnes Medal at
http://www.iucr.org/resources/commissions/electron-crystallography/gjonnes-medal

The Gj/PInnes Medal in Electron Crystallography is awarded every three
years and recognizes an outstanding contribution to the field of
electron crystallography. Nomination for the Gj/PInnes Medal is open to
scientists and engineers in all areas of electron crystallography
defined in the broadest context as the branch of science that uses
electron scattering and imaging to study the structure of matter.

Please forward as appropriate.


--
Professor Laurence Marks
Department of Materials Science and Engineering
Northwestern University
www.numis.northwestern.edu
Corrosion in 4D: MURI4D.numis.northwestern.edu
Co-Editor, Acta Cryst A
"Research is to see what everybody else has seen, and to think what
nobody else has thought"
Albert Szent-Gyorgi


==============================Original Headers==============================
6, 41 -- From marksmsa-at-gmail.com Mon Jan 25 07:56:48 2016
6, 41 -- Received: from mail-lb0-f169.google.com (mail-lb0-f169.google.com [209.85.217.169])
6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0PDul4Z011783
6, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 07:56:48 -0600
6, 41 -- Received: by mail-lb0-f169.google.com with SMTP id oh2so74306465lbb.3
6, 41 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 05:56:36 -0800 (PST)
6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 41 -- d=gmail.com; s=20120113;
6, 41 -- h=mime-version:date:message-id:subject:from:to:content-type
6, 41 -- :content-transfer-encoding;
6, 41 -- bh=eJTRCxW18amnX96ZxQah12BYXFKTQa7Ha1jEYoMZ138=;
6, 41 -- b=gyVIcug9pncp9WWAZoo5ERd9HCF60vuBClLojdqissl/LR3abuvNmGRVjv8Wcaxi7A
6, 41 -- 76pH4mAU/g90jRjjvrzNsjzY/leEx2i3J0F+X6eVtOB8m8KB+C6dYiPAM6tiXsZ8QnjC
6, 41 -- r1osfEtmJQCnQwvA9YcUTaLO5LObo+YfY1Sl5LcVz4PNTNtgZuyk0WbIHeJbfMjFkswH
6, 41 -- LxzO3k1mRiCTJS/iUpo4tEjMuiVgg8FtQr9IK0UhN02DMi3mMZlE5dCQ3PoKOu20hRu+
6, 41 -- 6uzh3R6QyOFSQ/T7C9b0dtm13kmegdsqGkuhYJLmM2xqcFO+avrEdP1LgbxrkiRu7boI
6, 41 -- Jfuw==
6, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 41 -- d=1e100.net; s=20130820;
6, 41 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to
6, 41 -- :content-type:content-transfer-encoding;
6, 41 -- bh=eJTRCxW18amnX96ZxQah12BYXFKTQa7Ha1jEYoMZ138=;
6, 41 -- b=Ny+1nrnvTJkt0t8NPQ2a7jTY8NAD1+5S9RbTT5RsNaGw6DPJIvbr2xo/58TVCiUlqW
6, 41 -- waI+VhDdyTu9jQRSZKm/ialtDWCddEW3TCg04OnkR0+mMeCHc3pXUvPlJRl0rYYiyllU
6, 41 -- IKQa/4FMJeJWJG0MWa3RLweZ/e3cyfgsaZ8Um33HTJROA3050DWq/2Al6itos3yc/9+U
6, 41 -- 6Kvgk6KOScryunGvpvBKI8DZqcGD/ZcCwUFKcNDPWhisEWME6+Rmr7cMsLqgzO9NkH/f
6, 41 -- 6kelqa9XxpaRRW+TtfOSS53cg0r5QTQ/beW+kvwm4ajTIhimGn/F2unmsBpDoQvFFa8z
6, 41 -- QhiA==
6, 41 -- X-Gm-Message-State: AG10YORXrJCnmVDOH8bqtrsumFzWaXn8w4NLkuUSDZUgHLcuxmnHmtHhmfMVDtZnivIvUy9DL8JiVTKL6TOHxA==
6, 41 -- MIME-Version: 1.0
6, 41 -- X-Received: by 10.112.73.41 with SMTP id i9mr1453083lbv.128.1453475883194;
6, 41 -- Fri, 22 Jan 2016 07:18:03 -0800 (PST)
6, 41 -- Received: by 10.112.126.228 with HTTP; Fri, 22 Jan 2016 07:18:03 -0800 (PST)
6, 41 -- Date: Fri, 22 Jan 2016 09:18:03 -0600
6, 41 -- Message-ID: {CAHuu12pZ6bd3S_XxFKtc6sBCFB3bT439A0zhhED7K+PV5cX7Fg-at-mail.gmail.com}
6, 41 -- Subject:
6, 41 -- From: L Marks {marksmsa-at-gmail.com}
6, 41 -- To: microscopy {Microscopy-at-microscopy.com}
6, 41 -- Content-Type: text/plain; charset=UTF-8
6, 41 -- Content-Transfer-Encoding: 8bit
6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0PDul4Z011783
==============================End of - Headers==============================




From: zackg-at-berkeley.edu
Date: Mon, 25 Jan 2016 20:53:59 -0600
Subject: [Microscopy] Stoichiometry Fitter app

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I wrote a software called stoichometry fitter for doing quick analyses of spectra acquired by EDS (or XRF).
Originally, this was just for my own use but it has evolved to the point where I think it may be useful for some of you. I have now made it open source and put it on GitHub for anyone who wants to use it or contribute to it.

As input it takes At%, Wt% or OxWt% values.

It has the ability to fit several phases, for example, if you have a pyroxene which you think is contaminated with a bit of FeS, you can fit against a pyroxene triangle + FeS, and it will give you the correct EnFsWo values after removing the appropriate amount of Fe for the FeS. It also has a freeform analysis capability for a few minerals I use, and I'm hoping others will contribute their own analyses as well so we can all use them. For example, if you tell it the spectrum is a pyroxene, then it will compute T, M1 and M2 sites for all the elements using the Morimoto 1988 reference. If you are handy with python and numpy then you can create your own analyses with a minimum of four lines of code.

Finally, it has a TEM k-factor + thickness correction capability if you input each element as counts instead of the usual At%/Wt%/OxWt%. This is very useful if you have a TEM, but it would also apply to some thin-film experiments in an SEM/EPMA.

I made a short overview video here:

https://berkeley.box.com/s/jxpfwh7gt8ixwfi7z6k1c67jslyd8c5r

And the project is here:

https://github.com/ZGainsforth/StoichiometryFitter

I hope this is helpful for some of you!

Cheers,

Zack

==============================Original Headers==============================
12, 46 -- From zackg-at-berkeley.edu Mon Jan 25 20:53:59 2016
12, 46 -- Received: from mail-pa0-f48.google.com (mail-pa0-f48.google.com [209.85.220.48])
12, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0Q2rxL8018876
12, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 20:53:59 -0600
12, 46 -- Received: by mail-pa0-f48.google.com with SMTP id ho8so90508673pac.2
12, 46 -- for {Microscopy-at-microscopy.com} ; Mon, 25 Jan 2016 18:53:47 -0800 (PST)
12, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 46 -- d=berkeley-edu.20150623.gappssmtp.com; s=20150623;
12, 46 -- h=from:content-type:content-transfer-encoding:subject:message-id:date
12, 46 -- :to:mime-version;
12, 46 -- bh=lw3PBT9Rrtu5f1dd6dEdfZhhyC4k64/ZceF5GLJ9dmo=;
12, 46 -- b=srQCv4b0RQiuY/Hd83vklPERlMfazNPy2sCVNHWKOH7JZUB/b7LXS4dIFEgV+LxwiR
12, 46 -- VwaR9yK+EIaj/AW/NR/HqkplmFWmPDMihStFRhTs/06T25JUdWP748u+4OwVxHayo0dG
12, 46 -- 77N9uc12F4DRPih4ifsj5iLsjJyBu/sr1V5LOD3utCxP2Fi/9sOwkw328yt8MqacukDf
12, 46 -- CDI0QAVT3o/1/NrT1I5+3oPa4vPXv8ytML8mnxRK1a4c6NfV+iGZ+6tbHh9vaiNJPEW1
12, 46 -- IIaSQHbV+wSm/w61nW0Q8jSIgahCH1fITs4e0FYcd0MsfztcOQwJ9zeN5FLGWWW2QroO
12, 46 -- diwg==
12, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 46 -- d=1e100.net; s=20130820;
12, 46 -- h=x-gm-message-state:from:content-type:content-transfer-encoding
12, 46 -- :subject:message-id:date:to:mime-version;
12, 46 -- bh=lw3PBT9Rrtu5f1dd6dEdfZhhyC4k64/ZceF5GLJ9dmo=;
12, 46 -- b=kt6DAxnnz16cxD3+/pTmzTVg1/WmYvgksRXRfCiFU+Pby4bHBJqnQLfux/n9l5dh9B
12, 46 -- 6XHEIr1rKqm7YnbbG/VJT3CWbZcOI4UR9XPqD/5KL+vrrcDbk377AQ01FTeS/IOG1QSM
12, 46 -- XsTkGrwlOl7RPABa4XzCuXDn9/5VVtvFMCIUo20bFfRK+rYEFFBhRqkWO/IWZPymSumK
12, 46 -- Sc/NZKVUC2lJXvh4TFlj3yFTt0zMSOJr+m0NF8dU5Ce//418sbS/0/D0AA+5H7FLvqcy
12, 46 -- 6oJXN+6ISZHSgCwHRCKA0bVVtH79QlvrGjUhF/llGvGjqDPnkwQvPcalOt8w2IWm7NKx
12, 46 -- dC4A==
12, 46 -- X-Gm-Message-State: AG10YOSfHozWmaCcvaaxpRwZzZFksEnvGeL+qduw39xhRAOVIKDLMJzu3lMvi3m8GhMf4D6o
12, 46 -- X-Received: by 10.66.235.231 with SMTP id up7mr30598779pac.7.1453776827006;
12, 46 -- Mon, 25 Jan 2016 18:53:47 -0800 (PST)
12, 46 -- Received: from [10.2.201.10] (ssl-air2.ssl.berkeley.edu. [128.32.13.9])
12, 46 -- by smtp.gmail.com with ESMTPSA id ze5sm31748212pac.32.2016.01.25.18.53.46
12, 46 -- for {Microscopy-at-microscopy.com}
12, 46 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 46 -- Mon, 25 Jan 2016 18:53:46 -0800 (PST)
12, 46 -- From: Zack Gainsforth {zackg-at-berkeley.edu}
12, 46 -- Content-Type: text/plain; charset=us-ascii
12, 46 -- Subject: Stoichiometry Fitter app
12, 46 -- Message-Id: {A0D3FF00-ABA2-4F6C-B072-7467AE155F38-at-berkeley.edu}
12, 46 -- Date: Mon, 25 Jan 2016 18:53:45 -0800
12, 46 -- To: Microscopy-at-microscopy.com
12, 46 -- Mime-Version: 1.0 (Mac OS X Mail 9.2 \(3112\))
12, 46 -- X-Mailer: Apple Mail (2.3112)
12, 46 -- Content-Transfer-Encoding: 8bit
12, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0Q2rxL8018876
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Jan 2016 06:51:29 -0600
Subject: [Microscopy] viaWWW: Gatan EELS & EFTEM Analysis Training School

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jhyun-at-gatan.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jhyun-at-gatan.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School

Message:
April 12-15, 2016
Gatan R&D Headquarters
Pleasanton, CA, USA

This comprehensive hands-on training course reviews the basic theory and practice of EELS imaging
and analysis in the TEM, but its main emphasis is on practical techniques, optimum deployment of
EELS and EFTEM hardware and software systems, and advanced applications. Some prior experience with
EELS, EFTEM, and energy filter systems is recommended, as is a good familiarity with TEM/STEM
instrumentation and techniques. By the end of the course, participants can expect to know how best
to optimize the performance of their EELS hardware as well as their EELS and EFTEM experimental
setups in order to capture and extract the maximum amount of information from their TEM samples.
Most of the curriculum is devoted to live microscope and computer lab sessions.

Topics:

-iFundamentals of EELS and energy-filtered imaging in TEM
-iPrinciples of operation of EFTEM and EELS systems
-iOptimization of EFTEM and EELS data acquisition
-iQuantification of elemental composition
-iOther information provided by EFTEM/EELS and how best to extract it
-iUse of EELS signals to form maps of elemental and chemical composition
-iEFTEM and STEM EELS spectrum imaging techniques
-iIdentification of material phases via EELS fine structure mapping
-iApplications to biological and physical science specimens

Complete information and online registration is available at:
http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2016



Login Host: 50.58.128.11
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
18, 53 -- From microscopy.listserver-at-gmail.com Tue Jan 26 06:51:29 2016
18, 53 -- Received: from mail-ig0-f175.google.com (mail-ig0-f175.google.com [209.85.213.175])
18, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0QCpTD5023880
18, 53 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jan 2016 06:51:29 -0600
18, 53 -- Received: by mail-ig0-f175.google.com with SMTP id t15so57829347igr.0
18, 53 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jan 2016 04:51:17 -0800 (PST)
18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
18, 53 -- d=gmail.com; s=20120113;
18, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
18, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
18, 53 -- bh=JlNJ9kuyT4OAb/MQcrCWl2TQ80r/jLARA5blyUSMzus=;
18, 53 -- b=j5BGO5VaMaZ2ynG15GduXA1F2bRjO+Lj7eKYDMIBF/s3GmzuWD2M344o05xsixeuPX
18, 53 -- y8dRH6TqgyTq5VNuM9qum0MifM85IdPrj6BirEgMpJGa8bCdN9jcVa+WqdSGTivFIcMw
18, 53 -- CtmcGKsAfZSNGqM2BDmc8DtHD1t7UjHjZ6tENADs6Dae0GVNvpqqzVR7Maq1EhXZ/w+7
18, 53 -- OCSl8CbxE/mrKZ9jFon+g1hRbDm6SkjwSA9AEQeq1QHoRdcZwD2Bkf47gjheYbwxILb9
18, 53 -- xemo2c+E5MzxXIAiEDl2SybGrPbPa1uN/xiEJ1bXrBE8BcwBvqEQYmcwF7FjyJnH3bLP
18, 53 -- cBlw==
18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
18, 53 -- d=1e100.net; s=20130820;
18, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
18, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
18, 53 -- :content-transfer-encoding;
18, 53 -- bh=JlNJ9kuyT4OAb/MQcrCWl2TQ80r/jLARA5blyUSMzus=;
18, 53 -- b=O7seTsqf6fLdY5uG3x52p9UPTzuzWfC41t6zkLsABgpplxv52/bVgBdBJnYZeVqngQ
18, 53 -- N4WPvDBa+T+Qv/qYJ/jrwrhjLKwX9bw8xxwuRbjji6Lt0crE51EfQGlJclP/SUmGYVLQ
18, 53 -- FQQ6A+OE89KwavnnV3/tY8gQKVW5A4/nCVkLtfEZf3BEQVa5J7Z7htam0RaQNUMi7fSo
18, 53 -- VCEYV8u0CrQTNzaM7Y8Z3XOEZYFHB5aCgb/mIBwNwtT4aULiJDOhJBbxjTY7xtJRbGVd
18, 53 -- Q4O9Ty6buvVwEl16QaCSj61Gkp1WN/SiAP7MEua1TxTiYqrUYuglw85ugiv6RQOSHsiM
18, 53 -- TiMw==
18, 53 -- X-Gm-Message-State: AG10YOTxHLNG0PrY/DdDngFjkdWy4dqa1bv9I4j2ayxDZTYn+qDYyPRkqxKp+q+z0f6rIw==
18, 53 -- X-Received: by 10.50.102.101 with SMTP id fn5mr23078272igb.62.1453812677175;
18, 53 -- Tue, 26 Jan 2016 04:51:17 -0800 (PST)
18, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
18, 53 -- by smtp.googlemail.com with ESMTPSA id o14sm1299274igi.21.2016.01.26.04.51.16
18, 53 -- for {microscopy-at-microscopy.com}
18, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
18, 53 -- Tue, 26 Jan 2016 04:51:16 -0800 (PST)
18, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
18, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
18, 53 -- {microscopylistserver-noreply-at-microscopy.com}
18, 53 -- Subject: viaWWW: Gatan EELS & EFTEM Analysis Training School
18, 53 -- References: {201601252317.u0PNHJn0013454-at-ns.microscopy.com}
18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
18, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
18, 53 -- X-Forwarded-Message-Id: {201601252317.u0PNHJn0013454-at-ns.microscopy.com}
18, 53 -- Message-ID: {56A76BC3.2050100-at-microscopy.com}
18, 53 -- Date: Tue, 26 Jan 2016 06:51:15 -0600
18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
18, 53 -- Gecko/20100101 Thunderbird/38.5.1
18, 53 -- MIME-Version: 1.0
18, 53 -- In-Reply-To: {201601252317.u0PNHJn0013454-at-ns.microscopy.com}
18, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed
18, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Jan 2016 06:52:24 -0600
Subject: [Microscopy] viaWWW: LEO440 CPU CMOS battery

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: david-at-electronmicro.com


This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both david-at-electronmicro.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: david-at-electronmicro.com
Name: David Scharf

Organization: SCHARF MICROSCOPY

Title-Subject: [Filtered] LEO440 CPU CMOS battery

Message: Can anyone help? I am getting a message that my CMOS battery has failed upon booting up my
LEO440 SEM. I have looked everywhere I can think of and cannot find that battery to replace it. It
is NOT on the computer board for sure! Must be external somewhere. Anyone service this instrument
before and know where its located? No mention of its location in my service manuals either. Thanks
in advance for any help.

Login Host: 45.48.249.22
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
13, 53 -- From microscopy.listserver-at-gmail.com Tue Jan 26 06:52:23 2016
13, 53 -- Received: from mail-ig0-f177.google.com (mail-ig0-f177.google.com [209.85.213.177])
13, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0QCqN0I024378
13, 53 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jan 2016 06:52:23 -0600
13, 53 -- Received: by mail-ig0-f177.google.com with SMTP id ik10so55881102igb.1
13, 53 -- for {microscopy-at-microscopy.com} ; Tue, 26 Jan 2016 04:52:12 -0800 (PST)
13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 53 -- d=gmail.com; s=20120113;
13, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
13, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
13, 53 -- bh=pv67TPepGTQ8Qs0pbIp4hayL/Xa3GxrLONsTtXrpxz4=;
13, 53 -- b=x3oqnpcO9h7xzf7rmx8XNI8s8bUM9p9aSuQh+iribFsB4h17DeUtzLo4BfBJehmVpP
13, 53 -- gv8J04op3q/EcWQDSx4b6uBmg3haXbAP4wUpV/YWN4bNxwbPZNc9EaJBeZuc3BQ2Jmpu
13, 53 -- k/sqfrGonIb3vG2ZMkiFT6FJhVGNnovTdz5KCVUq5V/VqX7c1nrAZ+x2rh1wa4/vY3Ro
13, 53 -- du3gCb7FaEWanuG925Fa3hc9c8ZCnkHnxaI5+r7TMGxZ1m4oeJleaB3XB1ykfRpqeofS
13, 53 -- LKEe82DAllIhFfvJb5Qu4xseBer66DFyyypj/DqOcnxMmixwp4kZDBO7sXH3o8atOY5m
13, 53 -- 7lrQ==
13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 53 -- d=1e100.net; s=20130820;
13, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
13, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
13, 53 -- :content-transfer-encoding;
13, 53 -- bh=pv67TPepGTQ8Qs0pbIp4hayL/Xa3GxrLONsTtXrpxz4=;
13, 53 -- b=Jn1+PqFfs1Dfo3sR/D6jRhRSBmg/RazXgqcJgCntR0V0KlwIhQhx7FS+Holbe2LnAL
13, 53 -- TZxLZEGFy/tlNuDYjAa78FQz3szROIvj5qQ401BAhyCn8vsrOmGBS69LAXAP/CizjBib
13, 53 -- s9XpE4IU3IrNDHvL7DJoAjoUtE4noCrpLw4pRvcd/mln/y94bRLmGj+sr9ygGDDVWR2u
13, 53 -- smFjCQNtLGVay9pX+EpTL+XWTt9qhxMFyg+RXM/GnFIcnKTOvMA0po49e+fBo/EG41ST
13, 53 -- 0wnVjSBqKMRb26XgshLVfdq4vvhoOKGqcnWkJaoh10Y+nyEbGlbWiTm3hyOkChqhT30+
13, 53 -- 3gxg==
13, 53 -- X-Gm-Message-State: AG10YOTGVBp0UpA9fQAt5TrubZvvPRjtq/3XpvNLa9lt9rxs/Tpc5EYooZ9p2d76h5VamA==
13, 53 -- X-Received: by 10.50.141.202 with SMTP id rq10mr8261171igb.49.1453812731827;
13, 53 -- Tue, 26 Jan 2016 04:52:11 -0800 (PST)
13, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
13, 53 -- by smtp.googlemail.com with ESMTPSA id ii1sm1007558igb.11.2016.01.26.04.52.11
13, 53 -- for {microscopy-at-microscopy.com}
13, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 53 -- Tue, 26 Jan 2016 04:52:11 -0800 (PST)
13, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
13, 53 -- {microscopylistserver-noreply-at-microscopy.com}
13, 53 -- Subject: viaWWW: LEO440 CPU CMOS battery
13, 53 -- References: {201601260926.u0Q9Q9sw016710-at-ns.microscopy.com}
13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
13, 53 -- X-Forwarded-Message-Id: {201601260926.u0Q9Q9sw016710-at-ns.microscopy.com}
13, 53 -- Message-ID: {56A76BFA.5080805-at-microscopy.com}
13, 53 -- Date: Tue, 26 Jan 2016 06:52:10 -0600
13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 53 -- Gecko/20100101 Thunderbird/38.5.1
13, 53 -- MIME-Version: 1.0
13, 53 -- In-Reply-To: {201601260926.u0Q9Q9sw016710-at-ns.microscopy.com}
13, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Jan 2016 07:26:51 -0600
Subject: [Microscopy] rviaWWW:TEM - Cryo-ultramicrotomy of rubber sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: badamianand-at-bfusa.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both badamianand-at-bfusa.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: badamianand-at-bfusa.com
Name: Anand Badami

Organization: Bridgestone Research

Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections

Message: Can anyone recommend a technique for collecting rubber sections during cryo-ultramicrotomy
that enables the sections to lie flat (i.e. as wrinkle free as possible) on a TEM support grid? I
currently collect my sections off the back of my cryoknife using a sucrose droplet, but as the
sections warm coming out of the cryochamber on the droplet they contract and wrinkle before being
deposited on a TEM grid (with or without carbon coating). Chamber temperature is -120 C. Any ideas
are appreciated. Thank you.

Login Host: 199.48.24.10
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

---===[|]===---



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
13, 53 -- From microscopy.listserver-at-gmail.com Wed Jan 27 07:26:51 2016
13, 53 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176])
13, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0RDQpUG006878
13, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 07:26:51 -0600
13, 53 -- Received: by mail-io0-f176.google.com with SMTP id d63so1646334ioj.2
13, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 05:26:39 -0800 (PST)
13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 53 -- d=gmail.com; s=20120113;
13, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
13, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
13, 53 -- bh=LTS5rNrfzBHWtSyM4Wmhs9ivjLl2E72SPsppBCPMuOA=;
13, 53 -- b=dTc9h4s0CksT3Ty6zOKfLIFt0tnPrynHIIvm2+Ccv3ZtMPCpzLn/ilTEsnaOEovCDh
13, 53 -- b/TutgqWX3ozf9+YALfGP0OPXYSOIlJK4d9kmrIV+66sQNbgLj6GvCfUmcwj5q4ko/ni
13, 53 -- UA3On0d8FP1UcW/RWxlHRJPnSZTv9X+eT2ZQBQ/nHHo5zuYDAkZB3HSzuqke0axxeISv
13, 53 -- mZ3CVGj9p7O8BmwmRMQEFdBeVXRISWOBDG5h13t2v1x7doxFJENlh/hPI7vOyCt6FEaE
13, 53 -- OrBgA5xN7kb/t+g+TCxTYyfjkN4baxmq26A37Ygr81r9H6yXb8woTFKTwRCKjeb5IX77
13, 53 -- IZRQ==
13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 53 -- d=1e100.net; s=20130820;
13, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
13, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
13, 53 -- :content-transfer-encoding;
13, 53 -- bh=LTS5rNrfzBHWtSyM4Wmhs9ivjLl2E72SPsppBCPMuOA=;
13, 53 -- b=g571CmhvoMtO1BonFE9jLIvtiSlsSTD6CWOHeKWpWsZO0pyn56CZt2SUTiG1dJGQ6r
13, 53 -- dm/lKUktGmwnoF71h06KpY9kyRF1X1/N4QU321ZtHwnupw6M3CKc41krVBI8S1gTU9PO
13, 53 -- 7Aco95X3PmQYiVQlGMZOvjzJ/uhNJo3Clk+d0snIr7rkz83uXtv9UcrEQwe65wuSdvWA
13, 53 -- cd9wAyvgdlFQFZPcd7BksLC2AkxHZxG9m5Yp8jRysKNg0hZ7P+4YvENnaOfpUSQk7jvH
13, 53 -- towZv3PojKLFw34H+exURZAd5N3u69in+gUEzPnjPMqe+VS5X28fku7/o0AMr+q98rp6
13, 53 -- QULA==
13, 53 -- X-Gm-Message-State: AG10YORi/cKngtL6P00oX3Vw19jj9x7Gkz2mPo2o18NK3DOTFwNilWkMkZbrQOZJWh3Y4w==
13, 53 -- X-Received: by 10.107.135.104 with SMTP id j101mr31894658iod.133.1453901199335;
13, 53 -- Wed, 27 Jan 2016 05:26:39 -0800 (PST)
13, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
13, 53 -- by smtp.googlemail.com with ESMTPSA id qd10sm3175885igc.1.2016.01.27.05.26.38
13, 53 -- for {microscopy-at-microscopy.com}
13, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 53 -- Wed, 27 Jan 2016 05:26:38 -0800 (PST)
13, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
13, 53 -- {microscopylistserver-noreply-at-microscopy.com}
13, 53 -- Subject: rviaWWW:TEM - Cryo-ultramicrotomy of rubber sections
13, 53 -- References: {201601261841.u0QIfPfC012543-at-ns.microscopy.com}
13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
13, 53 -- X-Forwarded-Message-Id: {201601261841.u0QIfPfC012543-at-ns.microscopy.com}
13, 53 -- Message-ID: {56A8C58D.7090802-at-microscopy.com}
13, 53 -- Date: Wed, 27 Jan 2016 07:26:37 -0600
13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 53 -- Gecko/20100101 Thunderbird/38.5.1
13, 53 -- MIME-Version: 1.0
13, 53 -- In-Reply-To: {201601261841.u0QIfPfC012543-at-ns.microscopy.com}
13, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: krassimir.bozhilov-at-ucr.edu
Date: Wed, 27 Jan 2016 10:16:53 -0600
Subject: [Microscopy] March 10th, 2016 symposium

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Symposium in Advanced Electron Microscopy in Nanostructure Research and Inauguration of the new Titan Themis 300 S/TEM.


We are glad to announce and invite you to a full-day symposium in iAdvanced Electron Microscopy in Nanostructure Researchi and the inauguration of the new Titan Themis 300 S/TEM and the Quanta 200 duo beam instrument at the Central Facility for Advanced Microscopy and Microanalysis (CFAMM).

The inauguration will take place on March 10th, 2016 starting at 9 a.m. in 355 HUB building at the University of California at Riverside. Plenary seminar talks will be presented by leading researchers demonstrating the application of S/TEM and duo-beam techniques in nanostructure research. The plenary session will be followed by demo sessions on the instruments in the afternoon.

Full program announcement to follow soon.

To register contact vcredadmin-at-ucr.edu.

//////////////////////////////////////////////////////////////////////////
Krassimir Bozhilov,

Central Facility for Advanced Microscopy and Microanalysis,
Research & Economic Development Office
Materials Science and Engineering - BCOE
University of California
Riverside, CA 92521

ph. 951 827 2998
fax 951 827 2489
bozhilov-at-ucr.edu
/////////////////////////////////////////////////////////////////////




==============================Original Headers==============================
11, 36 -- From krassimir.bozhilov-at-ucr.edu Wed Jan 27 10:16:53 2016
11, 36 -- Received: from mx2.ucr.edu (mx2.ucr.edu [138.23.62.3])
11, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0RGGqoJ001500
11, 36 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 10:16:53 -0600
11, 36 -- X-IronPort-Anti-Spam-Filtered: true
11, 36 -- X-IronPort-Anti-Spam-Result: A2DpAQAa7ahWmUD4F4pbA4R5BohRsymGDwKBTkwBAQEBAQESAQEBAQEICwsHISMLhEIBAQSBCQIBTjIlAgQTiBu6SgGDVQEBAQEBBQEBAQEBAQEBAQEBFYYLgW0Ig3eDHSaCZIEPBZZunD2OPYJxBIFXaodIAXsBAQE
11, 36 -- X-IPAS-Result: A2DpAQAa7ahWmUD4F4pbA4R5BohRsymGDwKBTkwBAQEBAQESAQEBAQEICwsHISMLhEIBAQSBCQIBTjIlAgQTiBu6SgGDVQEBAQEBBQEBAQEBAQEBAQEBFYYLgW0Ig3eDHSaCZIEPBZZunD2OPYJxBIFXaodIAXsBAQE
11, 36 -- X-IronPort-AV: E=Sophos;i="5.22,355,1449561600";
11, 36 -- d="scan'208";a="486292674"
11, 36 -- Received: from exch-edge-2.ucr.edu ([138.23.248.64])
11, 36 -- by smtp2.ucr.edu with ESMTP/TLS/AES256-SHA; 27 Jan 2016 08:16:24 -0800
11, 36 -- Received: from EXCH-HT-1.exch.ucr.edu (138.23.62.60) by exch-edge-2.ucr.edu
11, 36 -- (138.23.248.64) with Microsoft SMTP Server (TLS) id 14.3.248.2; Wed, 27 Jan
11, 36 -- 2016 08:16:17 -0800
11, 36 -- Received: from EXCH-MBOX-6.exch.ucr.edu ([169.254.6.219]) by
11, 36 -- EXCH-HT-1.exch.ucr.edu ([138.23.62.60]) with mapi id 14.03.0248.002; Wed, 27
11, 36 -- Jan 2016 08:16:24 -0800
11, 36 -- From: Krassimir Bozhilov {krassimir.bozhilov-at-ucr.edu}
11, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
11, 36 -- Subject: March 10th, 2016 symposium
11, 36 -- Thread-Topic: March 10th, 2016 symposium
11, 36 -- Thread-Index: AQHRWR4QoXyRkLBG4EW32F7n23W12Q==
11, 36 -- Date: Wed, 27 Jan 2016 16:16:23 +0000
11, 36 -- Message-ID: {7746EDDB-F715-4058-8CA2-DFE713A74A0D-at-ucr.edu}
11, 36 -- References: {162245AD-7BB7-4228-A150-A297AC2D0244-at-ucr.edu}
11, 36 -- In-Reply-To: {162245AD-7BB7-4228-A150-A297AC2D0244-at-ucr.edu}
11, 36 -- Accept-Language: en-US
11, 36 -- Content-Language: en-US
11, 36 -- X-MS-Has-Attach:
11, 36 -- X-MS-TNEF-Correlator:
11, 36 -- x-originating-ip: [138.23.63.83]
11, 36 -- Content-Type: text/plain; charset="Windows-1252"
11, 36 -- Content-ID: {BCD8221403869F4C80A209C6BDF7B0E5-at-exch.ucr.edu}
11, 36 -- MIME-Version: 1.0
11, 36 -- Content-Transfer-Encoding: 8bit
11, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0RGGqoJ001500
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Jan 2016 19:37:37 -0600
Subject: [Microscopy] viaWWW:TEM & SEM of intestinal cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: wtkiii-at-hotmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both wtkiii-at-hotmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: wtkiii-at-hotmail.com
Name: WILLIAM KING

Title-Subject: [Filtered] TEM & SEM of intestinal cells

Message: I would like to know why the antigen behind Ulcerative Colitis has not been identified.
If the affected tissue were available, how many micrographs would it take to provide some visual cues?

Login Host: 74.214.53.163
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
11, 53 -- From microscopy.listserver-at-gmail.com Wed Jan 27 19:37:36 2016
11, 53 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182])
11, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0S1baTM000502
11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 19:37:36 -0600
11, 53 -- Received: by mail-io0-f182.google.com with SMTP id g73so39893114ioe.3
11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 17:37:24 -0800 (PST)
11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 53 -- d=gmail.com; s=20120113;
11, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
11, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
11, 53 -- bh=o8nqqIZid4S6/jCLqLV//ibF0SYuiGxVdusJ17HL1EA=;
11, 53 -- b=zm5Hb6dKF/WZSGrHzVFwjgFUXJuft5XqrfjpbJgdQtWLxgSygs6+qqNmM/SFB+bZwN
11, 53 -- KOX9KYCxll2lrKOZZgV69Bq0YBq+nuKNVsVpgehE4OypHk73OEDz9MjPDfBgwt/axNBM
11, 53 -- SM+8eSBbqbGVNUNh/o9rU+ui/CuXxJnOTGBWROO6LAyW/JehNtYvC2Pnfqsy7U+9GkKL
11, 53 -- k+OBou5gYwYBmgUKlw993vgZeSJXDK7GZN0fC8vIrZu0CxeTkrl7vSItlIedpf00fUAR
11, 53 -- zhMh8ymiSGNor1zO0U7s3eVlUEOHhy2Oe0Te7qzOslVqF3cMvZNgPxPQtoky2/y+NXWU
11, 53 -- grMA==
11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 53 -- d=1e100.net; s=20130820;
11, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
11, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
11, 53 -- :content-transfer-encoding;
11, 53 -- bh=o8nqqIZid4S6/jCLqLV//ibF0SYuiGxVdusJ17HL1EA=;
11, 53 -- b=AYnfQ8MXLlXsdL48XlxnZnX6uGexqLyxESVz7OyKlwYXalNWc+qEiI27jLLjs4hGo5
11, 53 -- MC7NwsHfTnmRJj+F7CdbIA+AwcqsR8a1swNCW0bQapfZbi8KBmnh8QpUyLhmTpEFhGuw
11, 53 -- WDwBiJTNidEP0nTzwTMD5QtJb5KyFFF336ztogLT/VwC7xTOSskg6XW5O/1ckZy3FP45
11, 53 -- pii16RA4G4TjLRcIc2O3m6sc3yofidooKAKgLr55FrSoxw4/axyi/cc6ag9IXFLHREar
11, 53 -- p/YGUvjj3VTV9wqowzUWAOE/AALQ7n2mVDN/ogYQuMiMfJTIgzfThZ/QTgloBLE8tqNz
11, 53 -- BQLQ==
11, 53 -- X-Gm-Message-State: AG10YOSJyx16+91VsDgQnbmcCw4EULcvvNqg1I2vOxyrk+jYqa2qmS+zYfm9e7ThpTBJvA==
11, 53 -- X-Received: by 10.107.162.146 with SMTP id l140mr924133ioe.123.1453945044548;
11, 53 -- Wed, 27 Jan 2016 17:37:24 -0800 (PST)
11, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
11, 53 -- by smtp.googlemail.com with ESMTPSA id d9sm250201igx.5.2016.01.27.17.37.23
11, 53 -- for {microscopy-at-microscopy.com}
11, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
11, 53 -- Wed, 27 Jan 2016 17:37:24 -0800 (PST)
11, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
11, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
11, 53 -- {microscopylistserver-noreply-at-microscopy.com}
11, 53 -- Subject: viaWWW:TEM & SEM of intestinal cells
11, 53 -- References: {201601271851.u0RIpjDQ025247-at-ns.microscopy.com}
11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
11, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
11, 53 -- X-Forwarded-Message-Id: {201601271851.u0RIpjDQ025247-at-ns.microscopy.com}
11, 53 -- Message-ID: {56A970D3.2010200-at-microscopy.com}
11, 53 -- Date: Wed, 27 Jan 2016 19:37:23 -0600
11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
11, 53 -- Gecko/20100101 Thunderbird/38.5.1
11, 53 -- MIME-Version: 1.0
11, 53 -- In-Reply-To: {201601271851.u0RIpjDQ025247-at-ns.microscopy.com}
11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Jan 2016 19:38:44 -0600
Subject: [Microscopy] viaWWW:MSNO 2016 Winter meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: nxa157-at-case.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both nxa157-at-case.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: nxa157-at-case.edu
Name: Nanthawan Avishai

Organization: Microscopy Society of Northeastern Ohio, MSNO

Title-Subject: [Filtered] MSNO 2016 Winter meeting

Message: Our MSNO Winter Meeting 2016 will go to a new and exciting place: Lorain County Community
College. The meeting will be on March 2nd, 2016 3-7:15 pm. Please see the full program from attached
below.

The event will combine professional networking, lectures on by leading expert, guided tours & demos
at the impressive LCCC Desich Center, student posters, exhibitions by leading vendors in the field,
a nice dinner, a fun raffle and much more. Here are some of the highlights:
- Two main talks on Applications for MEMS Sensors (Matthew Apanius, Managing Director, SMART
Microsystems, Ltd) and "Basic Digital Imaging and Image Form" (by Dr. W. Gray Jerome, Vanderbilt
University Medical Center, a National MSA tour speaker).

- Tour at the Desich Center

The registration and more detail of this meeting can be found at
http://www.msneo.org/2016-winter-meeting.html

Looking forward to seeing you at LCCC!
MSNO Board

Login Host: 129.22.154.127
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
16, 53 -- From microscopy.listserver-at-gmail.com Wed Jan 27 19:38:41 2016
16, 53 -- Received: from mail-ig0-f170.google.com (mail-ig0-f170.google.com [209.85.213.170])
16, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0S1ceDt000866
16, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 19:38:40 -0600
16, 53 -- Received: by mail-ig0-f170.google.com with SMTP id z14so3388006igp.0
16, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Jan 2016 17:38:29 -0800 (PST)
16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 53 -- d=gmail.com; s=20120113;
16, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
16, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
16, 53 -- bh=Smiu4YVCmHDORVZxmeahtgWOt8pUoGPge09t49rm1o8=;
16, 53 -- b=GjSRdHE7nOLX2kDm5taBvQuVgPGBG+ids4GGTsOTSAzi4yOh4zkb0RzAk7hkdNNS5c
16, 53 -- r86IjgPtBnxLLjufTXKg0eY6UMb9wULNWj9FmrPWdixxNdwCiPXpHdZApoIGKKRw9IDI
16, 53 -- 5xaqHLeSHkMAqOYzH3xBfMI90SymEUoH+U5OZ7CFnNxgQeLksEk7zuJ9F2kwZQO047Cz
16, 53 -- XPx+TNTYxiOciExwE1L0Hx3RE183ysbizl/anZWPfh4TMQsakug5SY0uUDOnhbJzFOyt
16, 53 -- nhvzVzIjTyTA3LRnOpH+Ki7FyK9paWZe/Qri2x9H7Gqh9NMPSGvtWSpXI8+hCzHyFBoC
16, 53 -- ESDQ==
16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 53 -- d=1e100.net; s=20130820;
16, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
16, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
16, 53 -- :content-transfer-encoding;
16, 53 -- bh=Smiu4YVCmHDORVZxmeahtgWOt8pUoGPge09t49rm1o8=;
16, 53 -- b=UiE5mGsw8nJCBZw++dMqAT4rZlS7XzsPH7PGTkFZivWQCp+gMJ3KU5lW1xDLM8HveH
16, 53 -- cLxjT++Lese4JNmp6gXZ6UMJoKzGnfuLLhjviRHq9bkopdL3xRrcVzdZXf/FyWoSONsb
16, 53 -- T0WsX0716IsbD/b9vd14OLzh42GIUfkJ1SuwTJlxngbfRlsxOWp6cKXf7yGyfhWaLRsx
16, 53 -- 8EZJfbD7UP9Xq09yC//c8fzusXurgSUsRbK1ebEijw+7KPv5MhNj2Jfva4gNWT4rgK3T
16, 53 -- ra/AGEI+DUF/450i6V1oDawFoBpgPqyupCnUibHBb/YBqMoC03xA+ih3mwgwTAcyp4+P
16, 53 -- 2BAg==
16, 53 -- X-Gm-Message-State: AG10YORFEuf+PxZ6eCMq8GOVRHvpfohBL5/dyxPRF/k5Xh4qQhoKOy/tJ8fvpBgfNqnFhw==
16, 53 -- X-Received: by 10.50.61.209 with SMTP id s17mr331399igr.40.1453945108814;
16, 53 -- Wed, 27 Jan 2016 17:38:28 -0800 (PST)
16, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
16, 53 -- by smtp.googlemail.com with ESMTPSA id n11sm1011500ioe.29.2016.01.27.17.38.28
16, 53 -- for {microscopy-at-microscopy.com}
16, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 53 -- Wed, 27 Jan 2016 17:38:28 -0800 (PST)
16, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
16, 53 -- {microscopylistserver-noreply-at-microscopy.com}
16, 53 -- Subject: viaWWW:MSNO 2016 Winter meeting
16, 53 -- References: {201601271854.u0RIsasj025286-at-ns.microscopy.com}
16, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
16, 53 -- X-Forwarded-Message-Id: {201601271854.u0RIsasj025286-at-ns.microscopy.com}
16, 53 -- Message-ID: {56A97113.7030707-at-microscopy.com}
16, 53 -- Date: Wed, 27 Jan 2016 19:38:27 -0600
16, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
16, 53 -- Gecko/20100101 Thunderbird/38.5.1
16, 53 -- MIME-Version: 1.0
16, 53 -- In-Reply-To: {201601271854.u0RIsasj025286-at-ns.microscopy.com}
16, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
16, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: parishcm-at-ornl.gov
Date: Thu, 28 Jan 2016 06:52:27 -0600
Subject: [Microscopy] FEI SuperX / Bruker: Strange performance at very low count rates

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software).

I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me.

Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.)

I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples?

This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer.

Thanks in advance
Chad Parish

---------------------
Chad M. Parish, Ph.D.
Research and Development Staff Member
Radiation Effects and Microstructural Analysis Group
Materials Science and Technology Division
Oak Ridge National Laboratory
Phone: 1 865 574 0092
Email: parishcm-at-ornl.gov



==============================Original Headers==============================
10, 53 -- From parishcm-at-ornl.gov Thu Jan 28 06:52:26 2016
10, 53 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12])
10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SCqQkd021923
10, 53 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 06:52:26 -0600
10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 53 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116;
10, 53 -- t=1453985534; x=1485521534;
10, 53 -- h=from:to:subject:date:message-id:
10, 53 -- content-transfer-encoding:mime-version;
10, 53 -- bh=0K5YMDyPwYQooheRgxj/FTgKf0AHz08TVaqOkaSqnjk=;
10, 53 -- b=JQHfmgS1J7qCem6b4i9bUlY4nbNpNCehgsPzwhe5Ww+0fgUAvLHIzX2F
10, 53 -- HXJaUjP8fWBzrbD13bzEFwAE9DsHX320NirvzY78txNEbfxvWswwFMLz9
10, 53 -- j87523vFotYQsRFDnKxi2afbOHAkx5g4UkuIpYOXjuOKQG+SzzAdhjq+b
10, 53 -- 3pWaGGmnm9pGLVUkl893mvPox9XMfryEjuwEqrxo2b9KqKsXreT1HzNco
10, 53 -- yHl3l0oB3DLTLWU5JfeR4RcV4LyJs8g8YmQ2JWeSLl4ugPSrnbAkJwzgz
10, 53 -- AI/6xFhSZnwnR3fLyJx/Udz8vTLqC6DAa9wK8ncJSmFRD+kTEuMO90/n7
10, 53 -- w==;
10, 53 -- X-SG: RELAYLIST
10, 53 -- X-IronPort-AV: E=Sophos;i="5.22,358,1449550800";
10, 53 -- d="scan'208";a="96755483"
10, 53 -- Received: from emgwy1.ornl.gov ([160.91.254.9])
10, 53 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 28 Jan 2016 07:52:13 -0500
10, 53 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151])
10, 53 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits))
10, 53 -- (No client certificate requested)
10, 53 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3prhXF6W9Zz7tK9
10, 53 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jan 2016 07:52:13 -0500 (EST)
10, 53 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov
10, 53 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan
10, 53 -- 2016 07:52:13 -0500
10, 53 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS34.ornl.gov
10, 53 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan
10, 53 -- 2016 07:52:13 -0500
10, 53 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by
10, 53 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id
10, 53 -- 15.00.1104.000; Thu, 28 Jan 2016 07:52:13 -0500
10, 53 -- From: "Parish, Chad M." {parishcm-at-ornl.gov}
10, 53 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com}
10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates
10, 53 -- Thread-Topic: FEI SuperX / Bruker: Strange performance at very low count rates
10, 53 -- Thread-Index: AdFZyrKEswZ9BvjyTQmAzHMpryukEA==
10, 53 -- Date: Thu, 28 Jan 2016 12:52:12 +0000
10, 53 -- Message-ID: {16c8fb3650e14a9981accfc0506e11b5-at-EXCHCS32.ornl.gov}
10, 53 -- Accept-Language: en-US
10, 53 -- Content-Language: en-US
10, 53 -- X-MS-Has-Attach:
10, 53 -- X-MS-TNEF-Correlator:
10, 53 -- x-ms-exchange-transport-fromentityheader: Hosted
10, 53 -- x-originating-ip: [128.219.12.132]
10, 53 -- Content-Type: text/plain; charset="us-ascii"
10, 53 -- MIME-Version: 1.0
10, 53 -- Content-Transfer-Encoding: 8bit
10, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SCqQkd021923
==============================End of - Headers==============================




From: CGorman-at-hookecollege.com
Date: Thu, 28 Jan 2016 07:51:27 -0600
Subject: [Microscopy] Short Course Announcement: SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering its scanning electron microscopy short course March 14-18, 2016.*

In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.* For further SEM training details and registration information, please follow the link below:

https://www.mccrone.com/scanning-electron-microscopy-course


Best regards-
__________________________________________________
Chris Gorman
Admissions Specialist
Hooke College of Applied Sciences
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
630-887-7412(fax)
www.hookecollege.com




==============================Original Headers==============================
9, 42 -- From CGorman-at-hookecollege.com Thu Jan 28 07:51:23 2016
9, 42 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114])
9, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SDpLkj011667
9, 42 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 07:51:21 -0600
9, 42 -- X-ASG-Debug-ID: 1453989069-07bbd410f7ebb50001-4CH8be
9, 42 -- Received: from TMGEX1.tmg.mccrone.com ([192.168.101.73]) by spam.mccrone.com with ESMTP id aDMByiUpg4QvfiDR for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 07:51:09 -0600 (CST)
9, 42 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com
9, 42 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by
9, 42 -- TMGEX1.tmg.mccrone.com (192.168.101.73) with Microsoft SMTP Server (TLS) id
9, 42 -- 15.0.913.22; Thu, 28 Jan 2016 07:51:08 -0600
9, 42 -- Received: from TMGEX1.tmg.mccrone.com ([fe80::a05a:a09b:404e:c4a8]) by
9, 42 -- TMGEX1.tmg.mccrone.com ([fe80::a05a:a09b:404e:c4a8%14]) with mapi id
9, 42 -- 15.00.0913.011; Thu, 28 Jan 2016 07:51:08 -0600
9, 42 -- From: Christine Gorman {CGorman-at-hookecollege.com}
9, 42 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
9, 42 -- Subject: Short Course Announcement: SEM
9, 42 -- Thread-Topic: Short Course Announcement: SEM
9, 42 -- X-ASG-Orig-Subj: Short Course Announcement: SEM
9, 42 -- Thread-Index: AdFZ0vA92gqkX3u4SkywTHV9YXdzow==
9, 42 -- Date: Thu, 28 Jan 2016 13:51:08 +0000
9, 42 -- Message-ID: {fd98ebb68bf14edbb3aa003449e7a18c-at-TMGEX1.tmg.mccrone.com}
9, 42 -- Accept-Language: en-US
9, 42 -- Content-Language: en-US
9, 42 -- X-MS-Has-Attach:
9, 42 -- X-MS-TNEF-Correlator:
9, 42 -- x-originating-ip: [192.168.101.154]
9, 42 -- Content-Type: text/plain; charset="iso-8859-1"
9, 42 -- MIME-Version: 1.0
9, 42 -- X-Barracuda-Connect: UNKNOWN[192.168.101.73]
9, 42 -- X-Barracuda-Start-Time: 1453989069
9, 42 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi
9, 42 -- X-Barracuda-Scan-Msg-Size: 692
9, 42 -- X-Virus-Scanned: by bsmtpd at mccrone.com
9, 42 -- X-Barracuda-BRTS-Status: 1
9, 42 -- X-Barracuda-Spam-Score: 0.00
9, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests=
9, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.26533
9, 42 -- Rule breakdown below
9, 42 -- pts rule name description
9, 42 -- ---- ---------------------- --------------------------------------------------
9, 42 -- Content-Transfer-Encoding: 8bit
9, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SDpLkj011667
==============================End of - Headers==============================




From: rok210-at-lehigh.edu
Date: Thu, 28 Jan 2016 08:22:20 -0600
Subject: [Microscopy] Re: FEI SuperX / Bruker: Strange performance at very low

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chad,

we have a JEOL X-ray detector with a Thermo Pulse-processor and software
and we can go way down in beam current to still collect X-rays. On a
recent trip our engineer showed me a software switch to remove the
"noise" peak counts from the spectrum and the reported count rate.

But if you are literally not seeing any peaks with 200pA then there's a
fundamental problem - call Bruker at once.

Good luck.

Rob Keyse

On 1/28/2016 8:12 AM, parishcm-at-ornl.gov wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} One or two of my users have observed a strange behavior on our FEI Talos F200X (FEI SuperX detector, Bruker pulse processor, Bruker software).
}
} I'm not going to call this an "error" or a "fault," just something strange. Hopefully the list's hive mind can reality check me.
}
} Let's say that on a very thin, low-Z sample, 400 pA might give 400 counts / sec on each of the four detectors (as seen in the TIA SuperX tab). 300 pA might give 300 cps. 200 pA might give zero. (Numbers are approximate.)
}
} I interpret this to mean that there is a low-end discriminator on the pulse processor or software somewhere, and below a certain count rate no counts are registering. Is this possible? Is there a setting I can drill down to in order to let my users push to lower beam currents on their beam-sensitive samples?
}
} This isn't a common problem, most of my Talos users are running ~5 nA, ~200 kcps beautifully, but I want to help all my users, not just those who can use the STEM as an electron sledgehammer.
}
} Thanks in advance
} Chad Parish
}
} ---------------------
} Chad M. Parish, Ph.D.
} Research and Development Staff Member
} Radiation Effects and Microstructural Analysis Group
} Materials Science and Technology Division
} Oak Ridge National Laboratory
} Phone: 1 865 574 0092
} Email: parishcm-at-ornl.gov
}
}
}
} ==============================Original Headers==============================
} 10, 53 -- From parishcm-at-ornl.gov Thu Jan 28 06:52:26 2016
} 10, 53 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.12])
} 10, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SCqQkd021923
} 10, 53 -- for {Microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 06:52:26 -0600
} 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 10, 53 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116;
} 10, 53 -- t=1453985534; x=1485521534;
} 10, 53 -- h=from:to:subject:date:message-id:
} 10, 53 -- content-transfer-encoding:mime-version;
} 10, 53 -- bh=0K5YMDyPwYQooheRgxj/FTgKf0AHz08TVaqOkaSqnjk=;
} 10, 53 -- b=JQHfmgS1J7qCem6b4i9bUlY4nbNpNCehgsPzwhe5Ww+0fgUAvLHIzX2F
} 10, 53 -- HXJaUjP8fWBzrbD13bzEFwAE9DsHX320NirvzY78txNEbfxvWswwFMLz9
} 10, 53 -- j87523vFotYQsRFDnKxi2afbOHAkx5g4UkuIpYOXjuOKQG+SzzAdhjq+b
} 10, 53 -- 3pWaGGmnm9pGLVUkl893mvPox9XMfryEjuwEqrxo2b9KqKsXreT1HzNco
} 10, 53 -- yHl3l0oB3DLTLWU5JfeR4RcV4LyJs8g8YmQ2JWeSLl4ugPSrnbAkJwzgz
} 10, 53 -- AI/6xFhSZnwnR3fLyJx/Udz8vTLqC6DAa9wK8ncJSmFRD+kTEuMO90/n7
} 10, 53 -- w==;
} 10, 53 -- X-SG: RELAYLIST
} 10, 53 -- X-IronPort-AV: E=Sophos;i="5.22,358,1449550800";
} 10, 53 -- d="scan'208";a="96755483"
} 10, 53 -- Received: from emgwy1.ornl.gov ([160.91.254.9])
} 10, 53 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 28 Jan 2016 07:52:13 -0500
} 10, 53 -- Received: from EXCHOS31.ornl.gov (exchos31.ornl.gov [128.219.12.151])
} 10, 53 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits))
} 10, 53 -- (No client certificate requested)
} 10, 53 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 3prhXF6W9Zz7tK9
} 10, 53 -- for {Microscopy-at-Microscopy.Com} ; Thu, 28 Jan 2016 07:52:13 -0500 (EST)
} 10, 53 -- Received: from EXCHCS34.ornl.gov (128.219.12.148) by EXCHOS31.ornl.gov
} 10, 53 -- (128.219.12.151) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan
} 10, 53 -- 2016 07:52:13 -0500
} 10, 53 -- Received: from EXCHCS32.ornl.gov (128.219.12.146) by EXCHCS34.ornl.gov
} 10, 53 -- (128.219.12.148) with Microsoft SMTP Server (TLS) id 15.0.1104.5; Thu, 28 Jan
} 10, 53 -- 2016 07:52:13 -0500
} 10, 53 -- Received: from EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33]) by
} 10, 53 -- EXCHCS32.ornl.gov ([fe80::2408:8b8c:e63d:2a33%17]) with mapi id
} 10, 53 -- 15.00.1104.000; Thu, 28 Jan 2016 07:52:13 -0500
} 10, 53 -- From: "Parish, Chad M." {parishcm-at-ornl.gov}
} 10, 53 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-microscopy.com}
} 10, 53 -- Subject: FEI SuperX / Bruker: Strange performance at very low count rates
} 10, 53 -- Thread-Topic: FEI SuperX / Bruker: Strange performance at very low count rates
} 10, 53 -- Thread-Index: AdFZyrKEswZ9BvjyTQmAzHMpryukEA==
} 10, 53 -- Date: Thu, 28 Jan 2016 12:52:12 +0000
} 10, 53 -- Message-ID: {16c8fb3650e14a9981accfc0506e11b5-at-EXCHCS32.ornl.gov}
} 10, 53 -- Accept-Language: en-US
} 10, 53 -- Content-Language: en-US
} 10, 53 -- X-MS-Has-Attach:
} 10, 53 -- X-MS-TNEF-Correlator:
} 10, 53 -- x-ms-exchange-transport-fromentityheader: Hosted
} 10, 53 -- x-originating-ip: [128.219.12.132]
} 10, 53 -- Content-Type: text/plain; charset="us-ascii"
} 10, 53 -- MIME-Version: 1.0
} 10, 53 -- Content-Transfer-Encoding: 8bit
} 10, 53 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u0SCqQkd021923
} ==============================End of - Headers==============================

--
Robert Keyse, DPhil
Research Scientist
Lehigh University

5, East Packer Avenue,
Whitaker Laboratory
Bethlehem,
PA 18015-3194

Office (610)758-3465
Fax (610)758-4244


==============================Original Headers==============================
10, 33 -- From rok210-at-lehigh.edu Thu Jan 28 08:22:19 2016
10, 33 -- Received: from rain4.cc.lehigh.edu (rain4.cc.lehigh.edu [128.180.3.218])
10, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0SEMJmr000752
10, 33 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 08:22:19 -0600
10, 33 -- Received: from [127.0.0.1] (rok210pc.mat.lehigh.edu [128.180.54.183])
10, 33 -- (Authenticated sender: rok210)
10, 33 -- by rain4.cc.lehigh.edu (Postfix) with ESMTPSA id 0CEAA503AF87;
10, 33 -- Thu, 28 Jan 2016 09:22:07 -0500 (EST)
10, 33 -- Subject: Re: [Microscopy] FEI SuperX / Bruker: Strange performance at very low
10, 33 -- count rates
10, 33 -- To: parishcm-at-ornl.gov, microscopy-at-microscopy.com
10, 33 -- References: {201601281312.u0SDCisw001826-at-ns.microscopy.com}
10, 33 -- From: Robert Keyse {rok210-at-lehigh.edu}
10, 33 -- Message-ID: {56AA240E.7040000-at-lehigh.edu}
10, 33 -- Date: Thu, 28 Jan 2016 09:22:06 -0500
10, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101
10, 33 -- Thunderbird/38.5.1
10, 33 -- MIME-Version: 1.0
10, 33 -- In-Reply-To: {201601281312.u0SDCisw001826-at-ns.microscopy.com}
10, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 33 -- Content-Transfer-Encoding: 7bit
10, 33 -- X-Antivirus: avast! (VPS 160128-0, 01/28/2016), Outbound message
10, 33 -- X-Antivirus-Status: Clean
10, 33 -- X-Virus-Scanned: clamav-milter 0.98.7 at rain4.CC.lehigh.EDU
10, 33 -- X-Virus-Status: Clean
10, 33 -- X-Spam-Status: No, score=-4.0 required=5.0 tests=ALL_TRUSTED,LU_FORGED_SENDER,
10, 33 -- RP_MATCHES_RCVD autolearn=disabled version=3.4.1
10, 33 -- X-Spam-Report:
10, 33 -- * -5.0 ALL_TRUSTED Passed through trusted hosts only via SMTP
10, 33 -- * -0.0 RP_MATCHES_RCVD Envelope sender domain matches handover relay domain
10, 33 -- * 1.0 LU_FORGED_SENDER No description available.
10, 33 -- X-Spam-Checker-Version: SpamAssassin 3.4.1 (2015-04-28) on
10, 33 -- freddie.cc.lehigh.edu
==============================End of - Headers==============================




From: zaluzec-at-microscopy.com
Date: Thu, 28 Jan 2016 22:13:25 -0600
Subject: [Microscopy] viaWWW:FIB Technician Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: ming_j_zhang-at-amat.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both ming_j_zhang-at-amat.com as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: ming_j_zhang-at-amat.com
Name: Ming Zhang

Organization: Applied Materials Company

Title-Subject: [Filtered] FIB Technician Opening

Message: The successful candidate will focus on TEM sample preparations
by hands-on operations of ex-situ and in-situ lift-out systems.

Term:
Must be available for a minimum of 6 months. Term could be extended to
18 months. Highly possible to turn into a regular full time position in
6-12 months.

Work Schedule:
Weekend and night shifts.

Education:
Associate degree or equivalent combination of education and experience
in Engineering, Materials Science, Chemistry, or Physics, etc.

Experience:
Hands-on experience on FIBs or dual beams for TEM sample preparations.
Manual TEM sample prep a plus.

Other Skills:
Must be a team player and interact in a positive fashion with peers and
customers.
Must be able to work in a fast paced, multi-tasking environment where
priorities may change often.

Location:
Portland, Oregon.

US citizen or green card holder preferred.


Login Host: 152.135.235.188
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
=============================================
Do no rely to this messaage it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

==============================================

==============================Original Headers==============================
19, 55 -- From nestor.zaluzec-at-gmail.com Thu Jan 28 22:13:24 2016
19, 55 -- Received: from mail-pa0-f49.google.com (mail-pa0-f49.google.com [209.85.220.49])
19, 55 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0T4DOvb003928
19, 55 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 22:13:24 -0600
19, 55 -- Received: by mail-pa0-f49.google.com with SMTP id yy13so34306498pab.3
19, 55 -- for {microscopy-at-microscopy.com} ; Thu, 28 Jan 2016 20:13:12 -0800 (PST)
19, 55 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
19, 55 -- d=gmail.com; s=20120113;
19, 55 -- h=sender:from:subject:references:to:reply-to:message-id:date
19, 55 -- :user-agent:mime-version:in-reply-to:content-type
19, 55 -- :content-transfer-encoding;
19, 55 -- bh=iB43IzEno8m5JmrBgmiO6Q4kd3vSC+oThfyxKkrNniQ=;
19, 55 -- b=la0HQH3E7SLYjXf2EtKIG3bejZ1ny5Y4uWsFA95S6bcKuwTlqJzEmCmCpncRkOYsHD
19, 55 -- +0HlMVt9fLSQ0ke1kRi5Y1OBGMvn9i8wGGnosYkehJkIHxzdvS7MP4IJT3puOdUaSJTK
19, 55 -- FFchPCfxkoMT2jKihfq44fus4By8wng+Bte7XNW9xU8SlfcDvvmUb+6KKe9iuRcoC2HN
19, 55 -- Q8URuKiiiLdchEQma5QOjGDl+A3ZS5ArsIN5CwXD2432iE7427umHzDOJrShWGAM0X1f
19, 55 -- bQgiEm2i05E7/pJIn1TFMNtR/4mE2TMiAG7y0AsSGW28i2agef8AKvvtPbKMGuWA3Bbv
19, 55 -- 0KwA==
19, 55 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
19, 55 -- d=1e100.net; s=20130820;
19, 55 -- h=x-gm-message-state:sender:from:subject:references:to:reply-to
19, 55 -- :message-id:date:user-agent:mime-version:in-reply-to:content-type
19, 55 -- :content-transfer-encoding;
19, 55 -- bh=iB43IzEno8m5JmrBgmiO6Q4kd3vSC+oThfyxKkrNniQ=;
19, 55 -- b=JYJfgNisnQu5z6fyBFz2iny30sW4q0yUg1b32kID0J2P9CTztxrm3CZRaQ8y5mqIV0
19, 55 -- uRXjZMOkhAMci1JOvr/4lcnLK89OwpCpLXjkvEDxm+UBif8Av14W/Y/UuEOhFYyibm1g
19, 55 -- c6q+x6+kWk1zlOEgFxUV1sZfWM1bZty2LP3aWgruA5KospgIXdRHnyJkTl61s/pydT55
19, 55 -- oDRT8AWyDgytXpcUgAIQdTe1RqGGU7UlkS35Q48DFYsfw/3py3YSM0BkyNFz3WCKZsC+
19, 55 -- 45yX6jpYO5ITY8T3OFzDuickYxRhYKoHh3ekTyGUxBMCQwbusyN4c8F6IrzR3al7Hyi6
19, 55 -- 3ASA==
19, 55 -- X-Gm-Message-State: AG10YOSUd7tNfp9U/VkfuAt+9egCQ2fC2/B1hRvMKFUGHNVc53Dq5Su7kx+jhgHu5YjiRw==
19, 55 -- X-Received: by 10.66.194.230 with SMTP id hz6mr10236195pac.70.1454040792086;
19, 55 -- Thu, 28 Jan 2016 20:13:12 -0800 (PST)
19, 55 -- Received: from Nestors-MacBookAir-Pro-2014.local ([104.129.198.111])
19, 55 -- by smtp.googlemail.com with ESMTPSA id 11sm19727330pfq.87.2016.01.28.20.13.10
19, 55 -- for {microscopy-at-microscopy.com}
19, 55 -- (version=TLSv1/SSLv3 cipher=OTHER);
19, 55 -- Thu, 28 Jan 2016 20:13:10 -0800 (PST)
19, 55 -- Sender: "Nestor J. Zaluzec" {nestor.zaluzec-at-gmail.com}
19, 55 -- From: MicroscopyListserver-NoReply {zaluzec-at-microscopy.com}
19, 55 -- X-Google-Original-From: MicroscopyListserver-NoReply
19, 55 -- {microscopylistserver-noreply-at-microscopy.com}
19, 55 -- Subject: viaWWW:FIB Technician Opening
19, 55 -- References: {201601282300.u0SN02em029523-at-ns.microscopy.com}
19, 55 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
19, 55 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
19, 55 -- X-Forwarded-Message-Id: {201601282300.u0SN02em029523-at-ns.microscopy.com}
19, 55 -- Message-ID: {56AAE6D5.1090100-at-microscopy.com}
19, 55 -- Date: Thu, 28 Jan 2016 22:13:09 -0600
19, 55 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
19, 55 -- Gecko/20100101 Thunderbird/38.5.1
19, 55 -- MIME-Version: 1.0
19, 55 -- In-Reply-To: {201601282300.u0SN02em029523-at-ns.microscopy.com}
19, 55 -- Content-Type: text/plain; charset=windows-1252; format=flowed
19, 55 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Fri, 29 Jan 2016 19:42:54 -0600
Subject: [Microscopy] viaWWW:TEM: agar embedding cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Long ago, when histology was in vogue, I had a problem during the summer producing paraffin sections of good repute when the windows were full open, the temp was above 80, humidity was high and Summer was the bane of my progress.

If you have a fancy microtome that has a means of changing the angle of the knife, then you can refine the method that I used with a self-constructed series of thin, plywood templates that would permit me to change the setting of my blade as the weather moved thru its cycle. My goal, when the change was in the wind, was to produce a section whose height was greater than all the others I could produce with angles above and below the one that worked the best.

Air conditioning had just arrived, but not in our lab, so, I was stuck with my templates. Since I was working in a building with large windows, no cooling but the breeze on the mountain, patience and changing angles and, oh yes, changing the hardness of the paraffin as the weather went thru its yearly cycle. If one moved South to Florida to cut sections, one would have to fabricate a Florida paraffin mixture as an embedment - much too hard for even Spring or Fall in Bethlehem, PA.

So, we come to the advice. The first problem is compression. The second is the sharpness of the blade. The third is the vibrations that emanate from the microtome that can, in the first approximation, be reduced with a few drops of lubricant. Then, there is the vibration of the floor, the building, and the planet, and the solidity of the blade in its holder, the geometry of the blade and the angles of the cutting wedge on the edge of the blade. Sorry, I digress.

The optimal angle lies within a small range of not-so-optimal angles that are dependent on the hardness of the paraffin or plastic, the temp of the environment, the geometry of the cutting edge, the humidity, and everything else.

As I recall, my instructor, who had a PhD from Harvard under Alfred Romer, instructed me on the microtome and its personalities by advising me to, "fiddle with the block, the knife, the angle of the knife, the rate of the cutting cycle, and everything else until you get good sections." He ended with, "That's how I always did it."

Now that I've said and reviewed all of this I conclude that I have not been very helpful. That is primarily due to the fact that I haven't cut a section from a paraffin block since 1992, when I taught my younger daughter to cut sections for my research. As I recall, I used the word "fiddle" more than once during the tutorial, but again that was then and we sharpened our knives ourselves. She, by the way, was the best, and happiest 'cranker' I ever trained, and I convinced a small army to be good at it.

I hope that at the very least you are happy that you are younger, and don't have to do your sectioning in a room with the windows open - even in Winter!

Seicerely,

Fred Monson (he works on electron microscopes, hardly ever has to cut sections. and is contentedly moving toward 77 with good health and a lively sense of humor.

P.S. The adjustment of the knife angle is similar to the problem a baseball player has with his bat. He fiddles with everything to reduce the frequency of his pop ups. Neither are like the violin on which you are guaranteed that you will be world famous if you practice for 10,000 hours - either for the best or the worst noises you can project from the instrument.

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu
________________________________________
X-from: Kelli Goodkowsky via Histonet {histonet-at-lists.utsouthwestern.edu}
Sent: Friday, January 29, 2016 3:15 PM
To: histonet-at-lists.utsouthwestern.edu

X-from: sbarlow-at-mail.sdsu.edu
This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both sbarlow-at-mail.sdsu.edu as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: sbarlow-at-mail.sdsu.edu
Name: Steve Barlow

Organization: SDSU EM Facility

Title-Subject: [Filtered] TEM: agar embedding cells

Message: Some advice please on colorizing agar for embedding cells. In
the past, my osmicated pellets in agar blocks were easily trackable, but
when the cells are in a thin layer the cells don't give much contrast,
especially if not osmicated. I am collecting naked eukaryotic cells in
suspension culture, but have problems bring down the smaller cells by
centrifugation. Cultures containing cells are fixed in aldehyde or
aldehyde/osmium cocktail, collected onto millipore Nucleopore filters,
then held in place by adding warm agar over them on the filter.

When cooled, I peel off the filter, leaving me with a thin layer of
cells embedded in the agar. I then cut the agar layer into small blocks
to enhance fluid exchange during subsequent treatments. My problem is,
the cells are light colored to faintly black, and the thin layer of
cells does not stand out well, particularly when in the various stages
of epon infiltration/polymerization. I tried adding some azure II blue
dye I had on hand to the agar so I could see the agar blocks more
easily, but by the time I got up to epon/acetone, the color was gone.
Any suggestions for a dye that will withstand both the water based and
acetone solutions, or an alternative way to mark the little agar blocks
so I can more easily monitor them throughout the process?

Thanks in advance

Steve

Login Host: 146.244.234.237
Listserver Email Form V - 20120416
---------------------------------------------------------------------------




--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
16, 51 -- From microscopy.listserver-at-gmail.com Fri Jan 29 19:42:53 2016
16, 51 -- Received: from mail-pf0-f176.google.com (mail-pf0-f176.google.com [209.85.192.176])
16, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0U1gqAG010259
16, 51 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jan 2016 19:42:53 -0600
16, 51 -- Received: by mail-pf0-f176.google.com with SMTP id o185so46166740pfb.1
16, 51 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jan 2016 17:42:41 -0800 (PST)
16, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 51 -- d=gmail.com; s=20120113;
16, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
16, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
16, 51 -- bh=IpknVXH5dM2byTndC0vHV8JuiPLv4X8f+4qifdYYBQ4=;
16, 51 -- b=cS9+rcJ/YnMXqawqjn9U23nctJ/I8tB/cQ2yTFC2doJh8zw4Bb+G7yPAtxljGaa1AW
16, 51 -- et4uwHvB12b5HmypcSZC7jaMXZKExKXn9q8WNXrLSD/0spm2sv5g2m/Ga3fovz8h7MST
16, 51 -- Su4LLiLhwwFWMXUdXWC4AXByE+3JRJ1f5L6X5cOF5+rHgU6Pzc0XhUxhlUnw1cnGoeHz
16, 51 -- Qsnj3wBlLZ0E+aizSOexXoBp7CAAWWOcMYJwcFPIOFZeRzIessGHV+8Rj+NVyrbRfskB
16, 51 -- 5jFpcXqRJuiSTW69I0h845E1GmDqmPDEAUE3G5uRsymIYLT6hsV+9PHofvQpL9gzA7RO
16, 51 -- NWxA==
16, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 51 -- d=1e100.net; s=20130820;
16, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
16, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
16, 51 -- :content-transfer-encoding;
16, 51 -- bh=IpknVXH5dM2byTndC0vHV8JuiPLv4X8f+4qifdYYBQ4=;
16, 51 -- b=I4c1bOubj2vHL+lt4QmF9gKcEiuN2vZVU8o5qigT/l1rUBVA7AaofykwAd8hdKeF2o
16, 51 -- I8uNQyUtDQsKZuhcnFsJScZNR9PFdws84BB8QlqdO30Mv9k69DxFU3liPAVUDbYZNE58
16, 51 -- vQYnZomue08oHkLQNlJCLO57E9tNrr08a7kkEeG9NNLAcx9Oz6e1TXwlb+Xecz8bMahY
16, 51 -- FyqZLLXzn5At8JUwyqkUNKYygHislRMPp50xRHiQL8fo/Og8C/naqTJnGYRFEbd6HTL4
16, 51 -- mGYyPlXctw5N6F7zpH82CVQk2Lq2La2VDtAJHRW04liFnxaErDjYEwyfYcNypMi7+6BP
16, 51 -- h5kw==
16, 51 -- X-Gm-Message-State: AG10YOQMCKY2cpIKRenj6z45Q1VKFHnfrsBViuB4/O1dxVBClZ0rbIPYL1JNM0RLGEZmoA==
16, 51 -- X-Received: by 10.98.71.211 with SMTP id p80mr18136873pfi.135.1454118160424;
16, 51 -- Fri, 29 Jan 2016 17:42:40 -0800 (PST)
16, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
16, 51 -- by smtp.googlemail.com with ESMTPSA id 11sm26219751pfq.87.2016.01.29.17.42.38
16, 51 -- for {microscopy-at-microscopy.com}
16, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 51 -- Fri, 29 Jan 2016 17:42:40 -0800 (PST)
16, 51 -- Subject: viaWWW:TEM: agar embedding cells
16, 51 -- References: {201601291922.u0TJM5Ar015082-at-ns.microscopy.com}
16, 51 -- Reply-To: microscopy.listserver-at-gmail.com
16, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 51 -- X-Forwarded-Message-Id: {201601291922.u0TJM5Ar015082-at-ns.microscopy.com}
16, 51 -- Message-ID: {56AC1512.6000806-at-gmail.com}
16, 51 -- Date: Fri, 29 Jan 2016 19:42:42 -0600
16, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
16, 51 -- Gecko/20100101 Thunderbird/38.5.1
16, 51 -- MIME-Version: 1.0
16, 51 -- In-Reply-To: {201601291922.u0TJM5Ar015082-at-ns.microscopy.com}
16, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
16, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Fri, 29 Jan 2016 19:43:53 -0600
Subject: [Microscopy] viaWWW:Senior Scientist, Microscopy & Microanalysis - position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: vicenzie-at-si.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both vicenzie-at-si.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: vicenzie-at-si.edu
Name: Ed Vicenzi

Organization: Smithsonian Institution

Title-Subject: [Filtered] Senior Scientist, Microscopy & Microanalysis -
position available

Message: [16-LM-CR] Senior Scientist, Microscopy & Microanalysis. A Sr.
Microscopist will characterize widely diverse samples, including organic
and inorganic chemicals, household goods, pharmaceuticals, foods,
plastics, etc. Analyst must execute experimental studies and deliver
high quality data using a broad range of analytical methods including
SEM, EDX, FTIR microscopy, Polarized Light microscopy, Fluorescence,
Image analysis. Proficiency with sample preparation methods
(sectioning, mounting microtome, etc.) required. Familiarity with
GMP/GLP and excellent writing skills. PhD in Analytical Chemistry - 3+
years industrial experience, M.S. -n 5+ years industrial experience.
Background in Mineralogy a plus

Please direct questions regarding this position to:

========================
Jonathan Chun, Ph.D.
Alliance Technologies
9 Deer Park Dr. Suite B
Monmouth Jct., NJ 08852
732-355-1234 (ph)
jchun-at-alliancetechgroup.com
www.alliancetechgroup.com

Login Host: 129.6.126.112
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
15, 52 -- From microscopy.listserver-at-gmail.com Fri Jan 29 19:43:48 2016
15, 52 -- Received: from mail-pf0-f182.google.com (mail-pf0-f182.google.com [209.85.192.182])
15, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u0U1hl8W010440
15, 52 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jan 2016 19:43:47 -0600
15, 52 -- Received: by mail-pf0-f182.google.com with SMTP id x125so51210373pfb.0
15, 52 -- for {microscopy-at-microscopy.com} ; Fri, 29 Jan 2016 17:43:35 -0800 (PST)
15, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 52 -- d=gmail.com; s=20120113;
15, 52 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
15, 52 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 52 -- bh=ojFAWCsu5H4Z6ZpSwT8xuuBI87SnMP63YnKbKch5M4g=;
15, 52 -- b=me4DMjTsUXr85KJ2RuIX/D1Dv6IRpuuxhwhsFQ/jw4S5iGHsTQg7Px/UszKahjsU0y
15, 52 -- 7f5SiHrfsQIb4ixxl8E4Zy15nbODinzZFi/s54A3Uj/EgefZF/mXtB26E0TbKW4S5dCq
15, 52 -- zhtoZy17ZBas/SrRD8jr4UxeT8p5ScM0WpgTC/n/Jq53CsjmBHWkhhdVt5mIuJslwwCv
15, 52 -- rwQA3SgIoR0TZVWXTFPRzvrDrYNmuBSAG2z1FPL1dXbkjVgJTxRVenm5qQYUcwcNXk7J
15, 52 -- hxVMoruyLaXV7O4qX+VYsiHpKZQ0PknIV02r1rJq2sQvP/wkaWfDXqxx1xEtYWtMcYHi
15, 52 -- 82Cw==
15, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 52 -- d=1e100.net; s=20130820;
15, 52 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
15, 52 -- :date:user-agent:mime-version:in-reply-to:content-type
15, 52 -- :content-transfer-encoding;
15, 52 -- bh=ojFAWCsu5H4Z6ZpSwT8xuuBI87SnMP63YnKbKch5M4g=;
15, 52 -- b=kDgyhWe3Vn9PfowH0ykf13M5wuSOEUJIHCbQM+TQV9A974Gmmbe+PLGsAwTGloyvZW
15, 52 -- y89E3qjdbvelBnTZCkRmYW88WMw6OxZFCRusW/38NAoOdHOwp1BNNYaOmszT+rnPahid
15, 52 -- Z+z75YVwyxiTSaiH3Z0fiDZ6SI7wrHPw7XMPCx89WlOXaYxviJm+6QMf7tEP+l13O9KQ
15, 52 -- kBiKTCeF2DMmJHb2G8+IW5lYZ6kRWQyDgUMniCLG4ZIl4DSDYJ+JjtIb3FFw4pVnhGAl
15, 52 -- lAHuAaNdyXL77a0M9PF9jC29h8PHCKRMmG9+HLqCUO+wUrRvIrJDHraadpqBf180AsjF
15, 52 -- OSnQ==
15, 52 -- X-Gm-Message-State: AG10YOTagPKk4pXzAxxm/YOXY4om3Ft1Nxp9Ba74Mq2sOnwOWcmI2af5TYkWgDCy0rZ9kQ==
15, 52 -- X-Received: by 10.98.74.9 with SMTP id x9mr18480434pfa.14.1454118215374;
15, 52 -- Fri, 29 Jan 2016 17:43:35 -0800 (PST)
15, 52 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
15, 52 -- by smtp.googlemail.com with ESMTPSA id l14sm26241988pfb.73.2016.01.29.17.43.34
15, 52 -- for {microscopy-at-microscopy.com}
15, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 52 -- Fri, 29 Jan 2016 17:43:34 -0800 (PST)
15, 52 -- Subject: viaWWW:Senior Scientist, Microscopy & Microanalysis - position
15, 52 -- available
15, 52 -- References: {201601292226.u0TMQWcD006507-at-ns.microscopy.com}
15, 52 -- Reply-To: microscopy.listserver-at-gmail.com
15, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 52 -- X-Forwarded-Message-Id: {201601292226.u0TMQWcD006507-at-ns.microscopy.com}
15, 52 -- Message-ID: {56AC154A.8010509-at-gmail.com}
15, 52 -- Date: Fri, 29 Jan 2016 19:43:38 -0600
15, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
15, 52 -- Gecko/20100101 Thunderbird/38.5.1
15, 52 -- MIME-Version: 1.0
15, 52 -- In-Reply-To: {201601292226.u0TMQWcD006507-at-ns.microscopy.com}
15, 52 -- Content-Type: text/plain; charset=UTF-8; format=flowed
15, 52 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================






From: microscopy.listserver-at-gmail.com
Date: Mon, 1 Feb 2016 13:53:29 -0600
Subject: [Microscopy] viaWWW:TEM FEI low background double tilt holder tip repair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: 13qw9-at-queensu.ca

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both 13qw9-at-queensu.ca as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: 13qw9-at-queensu.ca
Name: Jason

Organization: Queen's University

Title-Subject: [Filtered] TEM FEI low background double tilt holder tip
repair

Message: Hi there,

We are encountering an issue with our FEI low background double tilt
holder. Probably because of some deformation on the holder tip, now the
two small pins cannot hold the sample cradle in position. Does anyone
have any experiences in fixing similar holders or know any company that
I can contact?

Thanks,

One colleague (Fei) has tried to send the same message with no luck. Not
sure what's the reason. Apologizes for letting you receive it twice.



Login Host: 199.7.157.107
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
17, 51 -- From microscopy.listserver-at-gmail.com Mon Feb 1 13:53:29 2016
17, 51 -- Received: from mail-pa0-f44.google.com (mail-pa0-f44.google.com [209.85.220.44])
17, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u11JrTWJ016086
17, 51 -- for {microscopy-at-microscopy.com} ; Mon, 1 Feb 2016 13:53:29 -0600
17, 51 -- Received: by mail-pa0-f44.google.com with SMTP id uo6so88837167pac.1
17, 51 -- for {microscopy-at-microscopy.com} ; Mon, 01 Feb 2016 11:53:17 -0800 (PST)
17, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 51 -- d=gmail.com; s=20120113;
17, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
17, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
17, 51 -- bh=Re8SsGI61lmaI1c1NIrWraRYGB8YR4wPGROMEy/4saY=;
17, 51 -- b=pnE5h+Eef2j0Y4lyRVJ+O5nupXAEPk9bbU+8qukpaeK0UyQbLRIDN42pXXpCSFvWjJ
17, 51 -- vLHofc6yKksmUz2MVmfLg2L5OoUfEbMD1o+MVXaZCz9TjtmH8feb9yAEyoJL2/V6LfwY
17, 51 -- WqgKWdVCRHB463tgJAJoDXKLr8wt9CDI2kloldT/+ckiJhIsJRcS7OC9hOAFcQMxhqlK
17, 51 -- hr9dBYfcmgqW+HrSaboUyXdR8lGi8r18n06XjG58HUzRmbEfRSPHS61GvTLKRGQbL212
17, 51 -- KSVQqNDrRwhXrl+tiBGoOawRuBJpVUP2nPHS33SrNEyLK7eifZYlZwvpfTciDkWQRtiH
17, 51 -- p2aw==
17, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 51 -- d=1e100.net; s=20130820;
17, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
17, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
17, 51 -- :content-transfer-encoding;
17, 51 -- bh=Re8SsGI61lmaI1c1NIrWraRYGB8YR4wPGROMEy/4saY=;
17, 51 -- b=d3oXKNjaHlM7951caKvRpurAji9djxZO50LXbcJM0w5XVVyUX4NbPYSQZ1HRHtjFzr
17, 51 -- aJOGFMsZbQrSA46Q7Fqns1ztOp+pFrIA6U409P+wUE70IvYu5KJp1hWA7jbhYsBr6sfW
17, 51 -- Jx4RuktCijrEN4EnMk0AAEmWAoE6VuIVVWxGaAAdPsetht35GUd9B+sk9qIpAiAOmUGV
17, 51 -- 7n8tgO04CMyfpS5OI0QpCAxbta/74YaVqRVHgl2w3zP3hllw3KaTv6W6j7OnEN7UaEMj
17, 51 -- DnTRGxbRdksgK0kuNSMk7pq8mvu2u/p6ScTHGDOGvydYOw+88/RkRTnmxN/bXLPlW4x7
17, 51 -- VpbQ==
17, 51 -- X-Gm-Message-State: AG10YOTGX+cQjyLSYyaiQayuJSYM2xJybbexybUbQdslViUCJnPAcdDkbzJDt+QSBH6n+g==
17, 51 -- X-Received: by 10.66.220.170 with SMTP id px10mr40801350pac.145.1454356396671;
17, 51 -- Mon, 01 Feb 2016 11:53:16 -0800 (PST)
17, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
17, 51 -- by smtp.googlemail.com with ESMTPSA id 76sm32878240pft.44.2016.02.01.11.53.14
17, 51 -- for {microscopy-at-microscopy.com}
17, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 51 -- Mon, 01 Feb 2016 11:53:15 -0800 (PST)
17, 51 -- Subject: viaWWW:TEM FEI low background double tilt holder tip repair
17, 51 -- References: {201602011839.u11IdjVj014060-at-ns.microscopy.com}
17, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 51 -- Reply-To: microscopy.listserver-at-gmail.com
17, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 51 -- X-Forwarded-Message-Id: {201602011839.u11IdjVj014060-at-ns.microscopy.com}
17, 51 -- Message-ID: {56AFB7A8.6070404-at-gmail.com}
17, 51 -- Date: Tue, 2 Feb 2016 06:53:12 +1100
17, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 51 -- Gecko/20100101 Thunderbird/38.5.1
17, 51 -- MIME-Version: 1.0
17, 51 -- In-Reply-To: {201602011839.u11IdjVj014060-at-ns.microscopy.com}
17, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
17, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: baskin-at-bio.umass.edu
Date: Mon, 1 Feb 2016 16:40:07 -0600
Subject: [Microscopy] Re: viaWWW:TEM: agar embedding cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I have had luck with fast green added at the 100% ethanol step. I
make a saturated fast green solution in ethanol and add a drop or two at
the 100% ethanol step. It kept the agar blocks rather colored through
subsquent infiltrations and polymerization. Sorry I don't have handy
what "saturated" means in terms of mg/mL but if you are interested I
could probably dig that up somewhere.
Good luck,
Tobias

On 1/29/16 8:44 PM, microscopy.listserver-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} X-from: sbarlow-at-mail.sdsu.edu
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both sbarlow-at-mail.sdsu.edu as well as the Microscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: sbarlow-at-mail.sdsu.edu
} Name: Steve Barlow
}
} Organization: SDSU EM Facility
}
} Title-Subject: [Filtered] TEM: agar embedding cells
}
} Message: Some advice please on colorizing agar for embedding cells. In
} the past, my osmicated pellets in agar blocks were easily trackable, but
} when the cells are in a thin layer the cells don't give much contrast,
} especially if not osmicated. I am collecting naked eukaryotic cells in
} suspension culture, but have problems bring down the smaller cells by
} centrifugation. Cultures containing cells are fixed in aldehyde or
} aldehyde/osmium cocktail, collected onto millipore Nucleopore filters,
} then held in place by adding warm agar over them on the filter.
}
} When cooled, I peel off the filter, leaving me with a thin layer of
} cells embedded in the agar. I then cut the agar layer into small blocks
} to enhance fluid exchange during subsequent treatments. My problem is,
} the cells are light colored to faintly black, and the thin layer of
} cells does not stand out well, particularly when in the various stages
} of epon infiltration/polymerization. I tried adding some azure II blue
} dye I had on hand to the agar so I could see the agar blocks more
} easily, but by the time I got up to epon/acetone, the color was gone.
} Any suggestions for a dye that will withstand both the water based and
} acetone solutions, or an alternative way to mark the little agar blocks
} so I can more easily monitor them throughout the process?
}
} Thanks in advance
}
} Steve
}
} Login Host: 146.244.234.237
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
}
}

--
__ ___ ^ ___ ___ Tobias I. Baskin
/ \ / / \ / \ Professor
/ / / / \ \ \ Biology Department
/ __/ /__ /___ \ \ \__ University of Mass.
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ Amherst, Massachusetts
/ /___ / \ \___/ \_____ USA 413-545-1533
www.bio.umass.edu/biology/baskin


==============================Original Headers==============================
4, 23 -- From baskin-at-bio.umass.edu Mon Feb 1 16:40:07 2016
4, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19])
4, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u11Me6ng007888
4, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 1 Feb 2016 16:40:07 -0600
4, 23 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10])
4, 23 -- (authenticated bits=0)
4, 23 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u11MdrUG010762
4, 23 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO);
4, 23 -- Mon, 1 Feb 2016 17:39:53 -0500
4, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
4, 23 -- Subject: Re: [Microscopy] viaWWW:TEM: agar embedding cells
4, 23 -- To: Microscopy-at-microscopy.com, sbarlow-at-mail.sdsu.edu
4, 23 -- References: {201601300144.u0U1irGV011027-at-ns.microscopy.com}
4, 23 -- Message-ID: {56AFDEB9.8020907-at-bio.umass.edu}
4, 23 -- Date: Mon, 1 Feb 2016 17:39:53 -0500
4, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
4, 23 -- Gecko/20100101 Thunderbird/38.5.1
4, 23 -- MIME-Version: 1.0
4, 23 -- In-Reply-To: {201601300144.u0U1irGV011027-at-ns.microscopy.com}
4, 23 -- Content-Type: text/plain; charset=windows-1252; format=flowed
4, 23 -- Content-Transfer-Encoding: 7bit
4, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Mon, 01 Feb 2016 17:39:53 -0500 (EST)
4, 23 -- X-Scanned-By: MIMEDefang 2.73
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Tue, 2 Feb 2016 05:00:37 -0600
Subject: [Microscopy] viaWWW:TEM: agar embedding cells

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steve!

Not directly answering your question but making a remark:

You are working with a cell monolayer, meaning approx. 50-um thin layer.
This is so thin that you don't have to care about fluid exchange, I don't think that cutting your agar in small blocks will add anything.
I would keep the whole agar block standing at the bottom of a jar, so you always know that the cells are on the upper side.

Regards
Stephane



--------------------------------------------
On Mon, 2/1/16, baskin-at-bio.umass.edu {baskin-at-bio.umass.edu} wrote:

Subject: [Microscopy] Re: viaWWW:TEM: agar embedding cells
To: nizets2-at-yahoo.com
Date: Monday, February 1, 2016, 11:48 PM




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor:-* The Microscopy
Society of America
To-* Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------

Hi,
-* -*-*-*I have had luck with fast green
added at the 100% ethanol step. I
make a saturated fast green solution in ethanol and add a
drop or two at
the 100% ethanol step. It kept the agar blocks rather
colored through
subsquent infiltrations and polymerization. Sorry I don't
have handy
what "saturated" means in terms of mg/mL but if you are
interested I
could probably dig that up somewhere.
Good luck,
-* -* -* -* -* -* -* -*
-* -* -* -*-*-*Tobias

On 1/29/16 8:44 PM, microscopy.listserver-at-gmail.com
wrote:
}
----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:-* The
Microscopy Society of America
} To-* Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
}
----------------------------------------------------------------------------
}
} X-from: sbarlow-at-mail.sdsu.edu
} This Question/Comment was submitted to the Microscopy
Listserver
} using the WWW based Form at
} http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
}
---------------------------------------------------------------------------
} Remember this posting is most likely not from a
Subscriber, so when
} replying
} please-* copy-* both sbarlow-at-mail.sdsu.edu
as well as-*-*-*the Microscopy
} Listserver
}
---------------------------------------------------------------------------
}
} Email: sbarlow-at-mail.sdsu.edu
} Name: Steve Barlow
}
} Organization: SDSU EM Facility
}
} Title-Subject: [Filtered] TEM: agar embedding cells
}
} Message: Some advice please on colorizing agar for
embedding cells.-* In
} the past, my osmicated pellets in agar blocks were
easily trackable, but
} when the cells are in a-* thin layer the cells
don't give much contrast,
} especially if not osmicated. I am collecting naked
eukaryotic cells in
} suspension culture, but have problems bring down the
smaller cells by
} centrifugation.-* Cultures containing cells are
fixed in aldehyde or
} aldehyde/osmium cocktail, collected onto millipore
Nucleopore filters,
} then held in place by adding warm agar over them on the
filter.
}
} When cooled, I peel off the filter, leaving me with a
thin layer of
} cells embedded in the agar.-* I then cut the agar
layer into small blocks
} to enhance fluid exchange during subsequent treatments.
My problem is,
} the cells are light colored to faintly black, and the
thin layer of
} cells does not stand out well, particularly when in the
various stages
} of epon infiltration/polymerization.-* I tried
adding some azure II blue
} dye I had on hand to the agar so I could see the agar
blocks more
} easily, but by the time I got up to epon/acetone, the
color was gone.
} Any suggestions for a dye that will withstand both the
water based and
} acetone solutions, or an alternative way to mark the
little agar blocks
} so I can more easily monitor them throughout the
process?
}
} Thanks in advance
}
} Steve
}
} -* -* Login Host: 146.244.234.237
} -* -* Listserver Email Form V - 20120416
}
---------------------------------------------------------------------------
}
}
}
}

--
-* -* -*-*-*__-* -*
___-*-*-*^-* -*
___-*-*-*___-*-*-*Tobias I. Baskin
-* -* -* /-* \-* /-*
-*-*-*/ \-* /-* -* -* \-*
-* -* Professor
-* -*-*-*/-*-*-*/ /-*
-*-*-*/-*-*-*\ \-* -*
-*-*-*\-* -* -* Biology Department
-* -* / __/ /__-*-*-*/___-* \
\-* -* -*-*-*\__-* -* University
of Mass.
-*-*-*/-* -*-*-*/-*
-*-*-*/-* -* -*-*-*\ \-*
-* -*-*-*\-* -* -* 611 N.
Pleasant St.
-* /-* -*-*-*/-*
-*-*-*/-* -* -* -*-*-*\
\-* -* -*-*-*\-* -* -*
Amherst, Massachusetts
/-* -*-*-*/___-* /-* -* -*
-* -*-*-*\ \___/-*-*-*\_____
USA-* 413-545-1533
-*-*-*www.bio.umass.edu/biology/baskin


==============================Original
Headers==============================
4, 23 -- From baskin-at-bio.umass.edu
Mon Feb-* 1 16:40:07 2016
4, 23 -- Received: from marlin.bio.umass.edu (bio.umass.edu
[128.119.55.19])
4, 23 -- -*-*-* by ns.microscopy.com
(8.12.11.20060308/8.12.8) with ESMTP id u11Me6ng007888
4, 23 -- -*-*-* for {Microscopy-at-microscopy.com} ;
Mon, 1 Feb 2016 16:40:07 -0600
4, 23 -- Received: from gouzibyte.bio.mor.nsm
(beutopia.bio.umass.edu [128.119.55.10])
4, 23 -- -*-*-* (authenticated bits=0)
4, 23 -- -*-*-* by marlin.bio.umass.edu
(8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id
u11MdrUG010762
4, 23 -- -*-*-* (version=TLSv1/SSLv3
cipher=DHE-RSA-AES128-SHA bits=128 verify=NO);
4, 23 -- -*-*-* Mon, 1 Feb 2016 17:39:53 -0500
4, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
4, 23 -- Subject: Re: [Microscopy] viaWWW:TEM: agar
embedding cells
4, 23 -- To: Microscopy-at-microscopy.com,
sbarlow-at-mail.sdsu.edu
4, 23 -- References: {201601300144.u0U1irGV011027-at-ns.microscopy.com}
4, 23 -- Message-ID: {56AFDEB9.8020907-at-bio.umass.edu}
4, 23 -- Date: Mon, 1 Feb 2016 17:39:53 -0500
4, 23 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X
10.11; rv:38.0)
4, 23 ---* Gecko/20100101 Thunderbird/38.5.1
4, 23 -- MIME-Version: 1.0
4, 23 -- In-Reply-To: {201601300144.u0U1irGV011027-at-ns.microscopy.com}
4, 23 -- Content-Type: text/plain; charset=windows-1252;
format=flowed
4, 23 -- Content-Transfer-Encoding: 7bit
4, 23 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed
by milter-greylist-4.3.9 (marlin.bio.umass.edu
[128.119.55.19]); Mon, 01 Feb 2016 17:39:53 -0500 (EST)
4, 23 -- X-Scanned-By: MIMEDefang 2.73
==============================End of -
Headers==============================


==============================Original Headers==============================
9, 31 -- From nizets2-at-yahoo.com Tue Feb 2 05:00:37 2016
9, 31 -- Received: from nm13-vm0.bullet.mail.bf1.yahoo.com (nm13-vm0.bullet.mail.bf1.yahoo.com [98.139.213.79])
9, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u12B0abC013725
9, 31 -- for {microscopy-at-microscopy.com} ; Tue, 2 Feb 2016 05:00:36 -0600
9, 31 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1454410824; bh=Dx1+gZFxK5gEwt4Z2zkyIr5WMWUTQT/f8DxSIiHV/lE=; h=Date:From:Reply-To:To:Cc:Subject:References:From:Subject; b=BasHxdKYqKCnHfyH+Sds7ZUq/sGPfc+4LyIqykwC75T12SDZGRyWeohEM9arlHMvmoa5mgRCaXbnkLs2/y3k5XixFKX6xjh1lJzbgq5zOfWuzfVDXqHetRyw7ZWpplTGOQxBoNlDewSedtqKqC4RtQVkU3ujwpudmPwKHx614vP51RCEZ9TbUu+LiNE+HAT/U6NlSOSKRZ7t3Z6frKGJigY5yP0hDDzAz/onFFtXehdacJWIqdD3DrfHPl1N33O2qiQx0d7DXOpbqajtk+lodmTWvkaIxgaNsI3mLUCti0IrkvW7o5ZMORT90EYfdLIfoTRiAbBJGXrwVMnnujnbBw==
9, 31 -- Received: from [98.139.170.181] by nm13.bullet.mail.bf1.yahoo.com with NNFMP; 02 Feb 2016 11:00:24 -0000
9, 31 -- Received: from [98.139.215.251] by tm24.bullet.mail.bf1.yahoo.com with NNFMP; 02 Feb 2016 11:00:24 -0000
9, 31 -- Received: from [127.0.0.1] by omp1064.mail.bf1.yahoo.com with NNFMP; 02 Feb 2016 11:00:24 -0000
9, 31 -- X-Yahoo-Newman-Property: ymail-3
9, 31 -- X-Yahoo-Newman-Id: 348360.42639.bm-at-omp1064.mail.bf1.yahoo.com
9, 31 -- X-YMail-OSG: 8j5OpNgVM1n5TXT6sxX3EdEfaHk6VvfRRtWo1jPgz_VDV6ycCV24neiuhqlm_Yr
9, 31 -- ywsjXB_SFuY.gXKpztISA9EurkYC8f7gYIbCqK2Jf7iaIDGt0oo8b5kQB1_HTKkwsShPE4DjXklU
9, 31 -- bJmK5NOqaOGNh3PZJBMRjL7jmsXiuidxksaoeRbXlPBWn8KEv1Nz8HHQyEJMExXTjUmx.JkpNwUh
9, 31 -- jPseSAbxJAN5QYmBW2xcP9hSsQIIFA6HLB61cYnNaBS3VO7iwLzlRQfuauxr650XYeosQFbJ9tJX
9, 31 -- TQAagHPs8W_1StrgmY6X5HkETpfVQPVXJV_rxEVI4quO6mP7711niJaT.7BnRqebXcyK9EBepJSw
9, 31 -- FDM5pqptR30Ux3vkAYMznsF_uE4XZDGOUTn8pJ9Xh5Oq1pnUlUpKhcopL74WTIjJBHnKWlo.m5l0
9, 31 -- 8jllfhmHAv_Q4eYg56tXLP7pTLB_4_0a5vE1Sl2kwN82F7KIuc4uTxEXfbrGjXnU1wcRgspwhm6Q
9, 31 -- KJ0tZVCIpumrGkw--
9, 31 -- Received: by 76.13.26.159; Tue, 02 Feb 2016 11:00:23 +0000
9, 31 -- Date: Tue, 2 Feb 2016 11:00:23 +0000 (UTC)
9, 31 -- From: Stephane Nizet {nizets2-at-yahoo.com}
9, 31 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
9, 31 -- To: {microscopy-at-microscopy.com}
9, 31 -- Cc: {sbarlow-at-mail.sdsu.edu}
9, 31 -- Message-ID: {1355926816.311073.1454410823450.JavaMail.yahoo-at-mail.yahoo.com}
9, 31 -- Subject: Re: [Microscopy] Re: viaWWW:TEM: agar embedding cells
9, 31 -- MIME-Version: 1.0
9, 31 -- Content-Type: text/plain; charset=UTF-8
9, 31 -- References: {1355926816.311073.1454410823450.JavaMail.yahoo.ref-at-mail.yahoo.com}
9, 31 -- Content-Transfer-Encoding: 8bit
9, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u12B0abC013725
==============================End of - Headers==============================




From: CGorman-at-hookecollege.com
Date: Tue, 2 Feb 2016 07:09:31 -0600
Subject: [Microscopy] Short Course Announcement: TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings Microscopists,

The Hooke College of Applied Sciences, located in Westmont, IL, is offering its transmission electron microscopy short course April 5-7, 2016.
In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment. For further TEM training details and registration information, please follow the link below:

https://www.mccrone.com/transmission-electron-microscopy-tem-course


Best regards-
__________________________________________________
Chris Gorman
Admissions Specialist
Hooke College of Applied Sciences
850 Pasquinelli Drive
Westmont, IL 60559-5539 USA
630-887-7100 (tel)
www.hookecollege.com


==============================Original Headers==============================
6, 42 -- From CGorman-at-hookecollege.com Tue Feb 2 07:09:30 2016
6, 42 -- Received: from spam.mccrone.com (mail.mccrone.com [12.54.22.114])
6, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u12D9Uw6004290
6, 42 -- for {microscopy-at-microscopy.com} ; Tue, 2 Feb 2016 07:09:30 -0600
6, 42 -- X-ASG-Debug-ID: 1454418558-07bbd410f71124f0001-4CH8be
6, 42 -- Received: from TMGEX2.tmg.mccrone.com ([192.168.101.54]) by spam.mccrone.com with ESMTP id dhgScWzqlv6aszwS for {microscopy-at-microscopy.com} ; Tue, 02 Feb 2016 07:09:18 -0600 (CST)
6, 42 -- X-Barracuda-Envelope-From: CGorman-at-hookecollege.com
6, 42 -- Received: from TMGEX1.tmg.mccrone.com (192.168.101.73) by
6, 42 -- TMGEX2.tmg.mccrone.com (192.168.101.54) with Microsoft SMTP Server (TLS) id
6, 42 -- 15.0.913.22; Tue, 2 Feb 2016 07:09:17 -0600
6, 42 -- Received: from TMGEX1.tmg.mccrone.com ([fe80::a05a:a09b:404e:c4a8]) by
6, 42 -- TMGEX1.tmg.mccrone.com ([fe80::a05a:a09b:404e:c4a8%14]) with mapi id
6, 42 -- 15.00.0913.011; Tue, 2 Feb 2016 07:09:17 -0600
6, 42 -- From: Christine Gorman {CGorman-at-hookecollege.com}
6, 42 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
6, 42 -- Subject: Short Course Announcement: TEM
6, 42 -- Thread-Topic: Short Course Announcement: TEM
6, 42 -- X-ASG-Orig-Subj: Short Course Announcement: TEM
6, 42 -- Thread-Index: AdFduuV3IcJtKGsFQJ6wowGGh2SEKQ==
6, 42 -- Date: Tue, 2 Feb 2016 13:09:17 +0000
6, 42 -- Message-ID: {05c929fdd6a748a3ab76b0cd5ba0b31a-at-TMGEX1.tmg.mccrone.com}
6, 42 -- Accept-Language: en-US
6, 42 -- Content-Language: en-US
6, 42 -- X-MS-Has-Attach:
6, 42 -- X-MS-TNEF-Correlator:
6, 42 -- x-originating-ip: [192.168.101.154]
6, 42 -- Content-Type: text/plain; charset="us-ascii"
6, 42 -- MIME-Version: 1.0
6, 42 -- X-Barracuda-Connect: UNKNOWN[192.168.101.54]
6, 42 -- X-Barracuda-Start-Time: 1454418558
6, 42 -- X-Barracuda-URL: https://spam.mccrone.com:443/cgi-mod/mark.cgi
6, 42 -- X-Barracuda-Scan-Msg-Size: 677
6, 42 -- X-Virus-Scanned: by bsmtpd at mccrone.com
6, 42 -- X-Barracuda-BRTS-Status: 1
6, 42 -- X-Barracuda-Spam-Score: 0.00
6, 42 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=5.0 KILL_LEVEL=7.0 tests=
6, 42 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.26674
6, 42 -- Rule breakdown below
6, 42 -- pts rule name description
6, 42 -- ---- ---------------------- --------------------------------------------------
6, 42 -- Content-Transfer-Encoding: 8bit
6, 42 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u12D9Uw6004290
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Feb 2016 14:25:19 -0600
Subject: [Microscopy] viaWWW:Job opening: Research Associate in Nuclear Graphite

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: h.wu2-at-lboro.ac.uk
This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both h.wu2-at-lboro.ac.uk as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: h.wu2-at-lboro.ac.uk
Name: Houzheng Wu

Organization: Loughborough University UK

Title-Subject: [Filtered] Job opening: Research Associate in Nuclear
Graphite

Message: Research Associate in Nuclear Graphite
http://www.jobs.ac.uk/job/AMX298/research-associate-in-nuclear-graphite/

Loughborough University - Department of Materials
Location: Loughborough, UK
Salary: #28,982 to #31,656 per annum
Hours: Full Time
Contract Type: Contract / Temporary
Placed on: 2nd February 2016
Closes: 7th February 2016
Job Ref: REQ15383

Fixed term for 33 months Understanding and Improving Graphite for
Advanced Nuclear Fission (UNIGRAF) Required to undertake experimental
research on structure from atomic- to micro-scale of iso-graphite for
advanced nuclear reactors. The project aims to improve the capability of
nuclear graphite through a better understanding of irradiation-induced
changes in structure and their impact on mechanical/physical behavior.
The post is part of an EPSRC funded project "UNIGRAF" in partnership
with Oxford and Bristol University, and multi supporters in USA, China
and Germany. Based at Loughborough the post holder will have strong
collaborations with scientists/engineers from all partners, and focus on
structure characterisation before and after irradiation using HRTEM,
EELS, FIB and other techniques. You will have opportunity to access
structural characterisation facilities in Oak Ridge National Laboratory,
Oxford, and EPSRC National Facility, and multi overseas travel is expected.

A good honours degree (2.1 minimum) and a PhD or equivalent in Materials
Science, Physics, or another relevant discipline and experience in
materials structural characterisation techniques is essential. Research
in graphite/graphene materials and knowledge in nuclear materials is
desirable. Application closing date: 7 February 2016


Login Host: 158.125.69.240
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
16, 51 -- From microscopy.listserver-at-gmail.com Tue Feb 2 14:25:19 2016
16, 51 -- Received: from mail-pf0-f171.google.com (mail-pf0-f171.google.com [209.85.192.171])
16, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u12KPICO005747
16, 51 -- for {microscopy-at-microscopy.com} ; Tue, 2 Feb 2016 14:25:19 -0600
16, 51 -- Received: by mail-pf0-f171.google.com with SMTP id o185so106044818pfb.1
16, 51 -- for {microscopy-at-microscopy.com} ; Tue, 02 Feb 2016 12:25:06 -0800 (PST)
16, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 51 -- d=gmail.com; s=20120113;
16, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
16, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
16, 51 -- bh=tiCnlAiqZ+STNBITA9AbUPOpLc+Wfais0weUkVnfrBA=;
16, 51 -- b=if/AOcj35iYjIFTOAT3BUrYudBbXxfeT+8dYu4t/CXpEwjeur4hlRvj2ZpKbbSO2m3
16, 51 -- AUnFEhq8uE2I/Qtm+kAH7d6JCKw6IRrdIwwI+CT5VUo+RTJC8xrdvea99ct2G/aZB0lW
16, 51 -- BoPbiExHW/WsddQEdDhIbXOqOYJWNJe9EA7RxMh6ylWQMUabWkVdul3cz3FJrMja1yL7
16, 51 -- nKU2EMDSvtQoVUMlZ/ihBFju+VMZw7FNLBp2pcsGFVkIgbJd10DwmkC6bq2Kv4ygxsCB
16, 51 -- 4wBN6FdUpMKeNPaLR2wVJw0hLCgPSMZYdOesKZK9hNI8ympqWbnSsijflYbYU3i1hDuy
16, 51 -- UgrQ==
16, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 51 -- d=1e100.net; s=20130820;
16, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
16, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
16, 51 -- :content-transfer-encoding;
16, 51 -- bh=tiCnlAiqZ+STNBITA9AbUPOpLc+Wfais0weUkVnfrBA=;
16, 51 -- b=k7ZkjRsQQXXSlBJHex8kA0EjVhLrxuj8k17WiKrUZJcHTHnw4uZ7CP985LZ59JOzv/
16, 51 -- FmZ5ImOgbxkGgJadCR0StA7DA4uDBRbyQrF/mPRSH67NYjm4EX8ftezcmvEkmTv0ywo3
16, 51 -- DzrO7JRDehsc7RXFpYs7iKxXuJp1U0rmt7kZEIE5HxfwkSAcYCcWwCtPTR5xOQDLTPvu
16, 51 -- +HB0XlN7wtrOInQnkvgWc9DAV96G0kKXwquKumxzCihAoXhbvNK2qLjw45R2kyPnCVoK
16, 51 -- c7sP6IXW9TyHXsAfgB7SIQkk39BvVAdTGem5f5fZGKNaxP+6bfG2QHh/j3uQEqPf2M2c
16, 51 -- rAOQ==
16, 51 -- X-Gm-Message-State: AG10YORyK+FuNQkgSWriBK4nToqzJBvPSg5hEQWshEeOi2oztcSVD+HIbZK9fmv8nrsIkw==
16, 51 -- X-Received: by 10.98.33.131 with SMTP id o3mr38820403pfj.97.1454444706394;
16, 51 -- Tue, 02 Feb 2016 12:25:06 -0800 (PST)
16, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
16, 51 -- by smtp.googlemail.com with ESMTPSA id dg1sm4600955pad.18.2016.02.02.12.25.04
16, 51 -- for {microscopy-at-microscopy.com}
16, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 51 -- Tue, 02 Feb 2016 12:25:05 -0800 (PST)
16, 51 -- Subject: viaWWW:Job opening: Research Associate in Nuclear Graphite
16, 51 -- References: {201602021734.u12HYZIG000665-at-ns.microscopy.com}
16, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 51 -- Reply-To: microscopy.listserver-at-gmail.com
16, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 51 -- X-Forwarded-Message-Id: {201602021734.u12HYZIG000665-at-ns.microscopy.com}
16, 51 -- Message-ID: {56B1109E.5000400-at-gmail.com}
16, 51 -- Date: Wed, 3 Feb 2016 07:25:02 +1100
16, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
16, 51 -- Gecko/20100101 Thunderbird/38.5.1
16, 51 -- MIME-Version: 1.0
16, 51 -- In-Reply-To: {201602021734.u12HYZIG000665-at-ns.microscopy.com}
16, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
16, 51 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Feb 2016 14:26:16 -0600
Subject: [Microscopy] viaWWW:Electron Microscopy Position at Naval Postgraduate School,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: skmeno1-at-nps.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both skmeno1-at-nps.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: skmeno1-at-nps.edu
Name: Sarath K Menon

Organization: NAVAL POSTGRADUATE SCHOOL

Title-Subject: [Filtered] Electron Microscopy Position at Naval
Postgraduate School, Monterey, CA

Message: Research Assistant Professor,
AD-1701-03
Department of Mechanical and Aerospace Engineering
Naval Postgraduate School, Monterey, CA

The Mechanical and Aerospace Engineering Department of the Naval
Postgraduate School is inviting applications from electron Microscopy
Specialists to fulfill a Research Faculty position to support the
research and development of a variety of materials including metals,
ceramics, carbon-based materials and composites. The position will be
focused on the operation of several advanced characterization
techniques, including fully equipped Scanning Electron Microscope and
Transmission Electron Microscope. Responsibilities of the position
include the maintenance and upkeep of these complex analytical
instruments. The applicant should possess operational expertise with
SEM, FIB, TEM lamellae preparation, TEM operation including STEM
methods, high resolution TEM and analytical techniques such as EDS,
EBSD, EELS and EFTEM. The candidate for the position must be able to
perform sample analysis with minimal direction and/or oversight from
technical principal investigators. The candidate must also be able to
collect, assimilate, and interpret data for technical principal
investigators and write technical reports to document results. The
position description includes also the coordination of all services
required to keep instruments operational, the procurement of all
consumables for the microscopy lab and to carry out all the routine
maintenance tasks. A PhD in Materials Science or a closely related field
is required. A few years of hands-on experience with current
state-of-the-art instrumentation and techniques in the area of electron
microscopy (SEM, TEM, STEM, EFTEM and FIB) is mandatory. The ability to
teach a course related to Advanced Materials Characterization techniques
will be highly valued. In addition, the candidate is expected to train
Masters level students to use instruments and help them with data
analysis. The ability to obtain a US security clearance is mandatory.

Applications should be received by 1 April 2015 and include a curriculum
vitae, writing sample, summary of available teaching evaluations, and
three letters of recommendation. Please address the applications to:

Garth V. Hobson
Professor and Chair
Department of Mechanical & Aerospace Engineering
700 Dyer Road, Bldg 245 -n Watkins Hall, WA-338
Naval Postgraduate School
Monterey, CA 93943
TEL: 831-656-2586/831-656-2888
www.nps.edu/mae
gvhobson-at-nps.edu



Salary is commensurate with qualifications and experience. Relocation
package, including recruitment/relocation incentive may be authorized.
The position will remain open until filled.
The Naval Postgraduate School is an equal opportunity employer. For
additional information about NPS, please refer to the website at
http://www.nps.edu.


Login Host: 205.155.65.226
Listserver Email Form V - 20120416
---------------------------------------------------------------------------




--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
21, 52 -- From microscopy.listserver-at-gmail.com Tue Feb 2 14:26:16 2016
21, 52 -- Received: from mail-pf0-f170.google.com (mail-pf0-f170.google.com [209.85.192.170])
21, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u12KQFvs006216
21, 52 -- for {microscopy-at-microscopy.com} ; Tue, 2 Feb 2016 14:26:15 -0600
21, 52 -- Received: by mail-pf0-f170.google.com with SMTP id w123so18247493pfb.0
21, 52 -- for {microscopy-at-microscopy.com} ; Tue, 02 Feb 2016 12:26:03 -0800 (PST)
21, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
21, 52 -- d=gmail.com; s=20120113;
21, 52 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
21, 52 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
21, 52 -- bh=dH/CXlUSVypUqRwjoROhb8Bya5hQFVKiD+5hhcVoGsw=;
21, 52 -- b=cnV3MZQCzOmeXrOVO0JunZboB+a5QjzLwKiVXCwjmBcvVUSmh9WWkjHZXnMMnKnOKJ
21, 52 -- Y8jzAAaD5lzaVH12z6Rc4oxCcfyigne3/gzC/3SDM4J/6+fKUm2Dxtl0QzZDNC5IQVNS
21, 52 -- 3BAUUdJQwtij8b90RfQlWrTbY0dKUzqBAMItKkDyKvHdDh3sO22cyoOw4rRE6DChT75y
21, 52 -- DzD/AbBTQAL6I70KlKvdIeBdTnLAjH6XGGoubZ6yHO1yCe4E3cPMZAIvM1cvc/wSyiO4
21, 52 -- Ia1kTn3aJt7g+vKrWE7G18HL5LtFKffX9J2dnPG+CngUlx/Kxmc9wAZxPW/pUSp6yMbx
21, 52 -- 7cIQ==
21, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
21, 52 -- d=1e100.net; s=20130820;
21, 52 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
21, 52 -- :date:user-agent:mime-version:in-reply-to:content-type
21, 52 -- :content-transfer-encoding;
21, 52 -- bh=dH/CXlUSVypUqRwjoROhb8Bya5hQFVKiD+5hhcVoGsw=;
21, 52 -- b=f77o00C3+lvdxiLShow08DAi8ugLfagclfu0OSpndgJ8xiIBBQmOpIc/ezdU7tlar1
21, 52 -- ma/812YuBsq4J2fx7/lH/nGdURN5ZkN9afjOlqqNPuZBUapPWX5qbJWkRztaPU7o7obU
21, 52 -- BbzJ8JOjnva539OiypoxlPbirPpoJAm0ZxjvJseZj/Qmpwlk4xCB3ogyH1iIAoeHE5TA
21, 52 -- QHkNM43mwWscxJnlApeptDT7Dgw68/Lnd2id/OTwolvuXqgy9jS9G+7L9SqqYTP/o8O+
21, 52 -- 0btqyJDc+3m+nVI6J2C7tVn1H5kiceu8wEGCQJJ4skaWzotvMs8DQ4hddOFU0cKgdOPF
21, 52 -- ZiUg==
21, 52 -- X-Gm-Message-State: AG10YOTr6j6qq+L38m1F6ku3tRgw8x7OtMEDfCQ9CM5n67Fp5FOANRRL2dmkfqknOsjMWg==
21, 52 -- X-Received: by 10.98.86.139 with SMTP id h11mr23596832pfj.105.1454444763232;
21, 52 -- Tue, 02 Feb 2016 12:26:03 -0800 (PST)
21, 52 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
21, 52 -- by smtp.googlemail.com with ESMTPSA id uk10sm4557218pab.31.2016.02.02.12.26.01
21, 52 -- for {microscopy-at-microscopy.com}
21, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
21, 52 -- Tue, 02 Feb 2016 12:26:02 -0800 (PST)
21, 52 -- Subject: viaWWW:Electron Microscopy Position at Naval Postgraduate School,
21, 52 -- Monterey, CA
21, 52 -- References: {201602020027.u120RaKN030235-at-ns.microscopy.com}
21, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
21, 52 -- Reply-To: microscopy.listserver-at-gmail.com
21, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
21, 52 -- X-Forwarded-Message-Id: {201602020027.u120RaKN030235-at-ns.microscopy.com}
21, 52 -- Message-ID: {56B110D6.8000008-at-gmail.com}
21, 52 -- Date: Wed, 3 Feb 2016 07:25:58 +1100
21, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
21, 52 -- Gecko/20100101 Thunderbird/38.5.1
21, 52 -- MIME-Version: 1.0
21, 52 -- In-Reply-To: {201602020027.u120RaKN030235-at-ns.microscopy.com}
21, 52 -- Content-Type: text/plain; charset=UTF-8; format=flowed
21, 52 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Feb 2016 16:10:15 -0600
Subject: [Microscopy] viaWWW:Calling all Graduates of Madison Area Technical College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: AThiessen-at-wisc.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both AThiessen-at-wisc.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: AThiessen-at-wisc.edu
Name: Anna Thiessen

Organization: Electron Microscopy Program/ Madison Area Technical College

Title-Subject: [Filtered] Calling all Graduates

Message: Hey Listservers we need your help!
The Electron Microscopy Program at Madison Area Technical College in
Madison, Wisconsin is closing its door after 24 years. The present and
past program directors (Michael Kostrna and Glenn Boda) are trying to
organize a reunion/-iwake-i for the program at the end of May.
Unfortunately, after 24 years a lot of contact information has been
changed and not updated! If you or anyone you know graduated or
participated in the program please pass the word on. If you would like
to find out more information, please contact Jennifer Kostrna at
kostrna.emwakeparty-at-gmail.com.

Many Thanks,
Anna Thiessen (Recent Graduate)

Login Host: 72.33.2.186
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
13, 51 -- From microscopy.listserver-at-gmail.com Tue Feb 2 16:10:13 2016
13, 51 -- Received: from mail-pa0-f46.google.com (mail-pa0-f46.google.com [209.85.220.46])
13, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u12MADEj018919
13, 51 -- for {microscopy-at-microscopy.com} ; Tue, 2 Feb 2016 16:10:13 -0600
13, 51 -- Received: by mail-pa0-f46.google.com with SMTP id yy13so1165181pab.3
13, 51 -- for {microscopy-at-microscopy.com} ; Tue, 02 Feb 2016 14:10:01 -0800 (PST)
13, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 51 -- d=gmail.com; s=20120113;
13, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
13, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
13, 51 -- bh=p8fw81FZY/c38XFyoEGJmM5L8vnF8l+tGcRxkbT//C8=;
13, 51 -- b=OlcXSjy1D+2RKkvQNwxFPVUfWfiEHUPcYLYOTz0Z7dm5rGYqvWcYsmyH5B0rsQD0en
13, 51 -- Vdps0slL1+TAyRRHVpKgXQraq+PPpp6iUcNKtidloY5yxh3FbDjClSdK9fY/MdKLjCix
13, 51 -- WhhraEKgYe+a/KjTV8MTBJN0UmQJsdreIlCXpQpOt94p7R9mqCzMEwuENXS+XwwDl9Ni
13, 51 -- FcE+4GnrrpPHdvKMoA11OWJ4EFebj8uisBNc+i62VxWSPf5EWXgVDpogfBAPW3HQbQDZ
13, 51 -- qLH4GZ8HiOF4Za4KEY+isaqXpxwk6We9GdzM7p7VbO0ploP9NQkSZvSDOzJgoA5/c3T9
13, 51 -- d3EA==
13, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 51 -- d=1e100.net; s=20130820;
13, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
13, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
13, 51 -- :content-transfer-encoding;
13, 51 -- bh=p8fw81FZY/c38XFyoEGJmM5L8vnF8l+tGcRxkbT//C8=;
13, 51 -- b=QcttUg+S0W5NNo4VTH62XwphKXVkBx851jgWoIy6owpjEc87nkQwZFUSp3CsqpCpEC
13, 51 -- QYfek8fdbUYL05+Vw2zIrsvY7lORN8FPm+Lmt+D6X+xrYOgKnSAU6ftMauej3h0qMXl7
13, 51 -- pbG94u9XQwmhguFd1sKLnOVnQJkqXBU2AksK00uCXU9UHxVOc1Yriy8JOvThLcSGVTpE
13, 51 -- S0E2rzO2fEiHpTftTwei+Y/wZyhC2vSdOiVvyrGUs4ox7WMOzSPi28kZj+od+gtqGxDn
13, 51 -- oHvOXGia+pLI2WSbQe4bL+TVQMpZKsWuo4TSSyZiwcs8IGV2kMf7HzKmhRCtD6uUjz7A
13, 51 -- OT9g==
13, 51 -- X-Gm-Message-State: AG10YOQ+2mUiQEZfJiGdkOqXYbCZx4GTxBJmP8vkI6Omt6rCnaIx9Z1JFi/xB4l2Gt1wIg==
13, 51 -- X-Received: by 10.66.121.136 with SMTP id lk8mr50523105pab.1.1454451000794;
13, 51 -- Tue, 02 Feb 2016 14:10:00 -0800 (PST)
13, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([203.56.3.40])
13, 51 -- by smtp.googlemail.com with ESMTPSA id tv6sm4934148pab.4.2016.02.02.14.09.58
13, 51 -- for {microscopy-at-microscopy.com}
13, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 51 -- Tue, 02 Feb 2016 14:10:00 -0800 (PST)
13, 51 -- Subject: viaWWW:Calling all Graduates of Madison Area Technical College
13, 51 -- References: {201602022148.u12LmY3d017275-at-ns.microscopy.com}
13, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 51 -- Reply-To: microscopy.listserver-at-gmail.com
13, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 51 -- X-Forwarded-Message-Id: {201602022148.u12LmY3d017275-at-ns.microscopy.com}
13, 51 -- Message-ID: {56B12931.6020001-at-gmail.com}
13, 51 -- Date: Wed, 3 Feb 2016 09:09:53 +1100
13, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 51 -- Gecko/20100101 Thunderbird/38.5.1
13, 51 -- MIME-Version: 1.0
13, 51 -- In-Reply-To: {201602022148.u12LmY3d017275-at-ns.microscopy.com}
13, 51 -- Content-Type: text/plain; charset=UTF-8; format=flowed
13, 51 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Feb 2016 05:00:28 -0600
Subject: [Microscopy] viaWWW:Free Kevex Sigma EDX System for SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Hobie-at-technicalsalessolutions.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both Hobie-at-technicalsalessolutions.com as well as the
Microscopy Listserver
---------------------------------------------------------------------------

Email: Hobie-at-technicalsalessolutions.com
Name: Hobie Richards

Organization: Technical Sales Solutions

Title-Subject: [Filtered] Free Kevex Sigma EDX System for SEM

Message: TSS has one complete Kevex Sigma EDX system, that needs a good
home. Detector (window looks good), collimator, Sigma Analyzer, 4855
Digital Beam Control Interface Module, and cable set. Was installed on
XL 30 W SEM.

Pictures may be seen here:
https://tss.exavault.com/share/view/a4yp-7l4eat7f

Will put in a box and with your shipping label send to you anywhere in
the world.

Login Host: 173.164.81.170
Listserver Email Form V - 20120416
---------------------------------------------------------------------------
--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
12, 51 -- From microscopy.listserver-at-gmail.com Wed Feb 3 05:00:28 2016
12, 51 -- Received: from mail-pf0-f169.google.com (mail-pf0-f169.google.com [209.85.192.169])
12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u13B0Rch026521
12, 51 -- for {microscopy-at-microscopy.com} ; Wed, 3 Feb 2016 05:00:27 -0600
12, 51 -- Received: by mail-pf0-f169.google.com with SMTP id 65so12178090pfd.2
12, 51 -- for {microscopy-at-microscopy.com} ; Wed, 03 Feb 2016 03:00:15 -0800 (PST)
12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=gmail.com; s=20120113;
12, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
12, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 51 -- bh=wVBgNHakHaePJrsLlZeXiMx7w/14HRauaY4Lkx6tlrs=;
12, 51 -- b=NGiFki2OFOCgqdK3E3eBuPA4wLkO9yrNue3XPVQO9sKHY6CldYN/XQ2wIv9mFa1Dj/
12, 51 -- ps12wNIRPby1T0ALT27tq4HJ6D8gYDCdfFaDlcgvj3iCDz/Xdz2gi5f462K7ys4klssg
12, 51 -- qBkJMQaVcj/PKi2/6cO7yey0EHNDBpgxYxxKOv4+Gy1G5r3yf/N5iaVcWcZCtw7yLnT9
12, 51 -- we2rBHdz/lmkauq8WPZX9tDoCIrqv0fO3OULm1yxrLbucE6LkVwPO5tnjvRCjmZkM9x0
12, 51 -- ne/mGOgnTEhzetKiOHvSob04sYuDCXg2+TYPmPzheIXHZ4tI6iCQnyxe3wCQLWiwUB8a
12, 51 -- cTLQ==
12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=1e100.net; s=20130820;
12, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
12, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 51 -- :content-transfer-encoding;
12, 51 -- bh=wVBgNHakHaePJrsLlZeXiMx7w/14HRauaY4Lkx6tlrs=;
12, 51 -- b=L7KiCXFZe3HzSMtDPjXs2PsVs80gwA8Lds9clySWJoFzFtbIpCLmeuHebKZcf2pzKN
12, 51 -- /8hVP0fVnTIf7pT85DTTssZ4V2nx0rCf3h0rQOZPtMo1RyELfgzRlAx7Fo88Bocx0HMy
12, 51 -- y11PPOVA2Dg01yHsbzYCm85+Qz41rV9DUS4MssKwFe6VfB44i5sPmvI2XDhUjqxDsbfH
12, 51 -- 0ETMmGOtlIKdLkdOBmd5jz0AD/plKpgz0d3TjJfkXc/HUec1jfkVRfbTSYI55FYTXlEp
12, 51 -- epikp1GG301lGBGWhYsu5aQhrXlA7rugg8iOrtilhz+6SUxH24VIXuLr71XwTFvjOLpy
12, 51 -- d1dA==
12, 51 -- X-Gm-Message-State: AG10YOStlTC+SoMkj9eHdVZsikb03cMmrjhimEj6MxxaUO/xTer2Os5qqIlyljuUx/gKQg==
12, 51 -- X-Received: by 10.98.16.86 with SMTP id y83mr1129142pfi.45.1454497215299;
12, 51 -- Wed, 03 Feb 2016 03:00:15 -0800 (PST)
12, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
12, 51 -- by smtp.googlemail.com with ESMTPSA id u21sm9080364pfi.15.2016.02.03.03.00.13
12, 51 -- for {microscopy-at-microscopy.com}
12, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 51 -- Wed, 03 Feb 2016 03:00:14 -0800 (PST)
12, 51 -- Subject: viaWWW:Free Kevex Sigma EDX System for SEM
12, 51 -- References: {201602022228.u12MS189026336-at-ns.microscopy.com}
12, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 51 -- Reply-To: microscopy.listserver-at-gmail.com
12, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 51 -- X-Forwarded-Message-Id: {201602022228.u12MS189026336-at-ns.microscopy.com}
12, 51 -- Message-ID: {56B1DDBB.50304-at-gmail.com}
12, 51 -- Date: Wed, 3 Feb 2016 22:00:11 +1100
12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
12, 51 -- Gecko/20100101 Thunderbird/38.5.1
12, 51 -- MIME-Version: 1.0
12, 51 -- In-Reply-To: {201602022228.u12MS189026336-at-ns.microscopy.com}
12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
12, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 4 Feb 2016 13:37:18 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: debrat-at-bcm.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both debrat-at-bcm.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM
sections. I filter the primary fix mixture with a 0.1um vacuum
filtration unit; I've tried changing the water and am currently using
RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use
Spurr's Low Viscosity embedding resin. Most recently I have been
ethanol-washing all razor blades used during specimen collection; I use
a 0.04% FSG quench after fixation (didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see
it in other tissues as well. I just looked at some freshly cut,
unstained mouse foot pad muscle yesterday and there were the black
speckles all over the place! The specks are tiny, much smaller than
10nm. It almost looks like immuno-gold, except that I haven't done
immuno-gold staining in years, and the specks are not rounded as you
might expect with gold particles. They don't seem to be clustered at any
specific organelle, and they are definitely in the tissue, not on the
surface. Someone please help, this is driving me nuts!

Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
12, 51 -- From microscopy.listserver-at-gmail.com Thu Feb 4 13:37:17 2016
12, 51 -- Received: from mail-pf0-f179.google.com (mail-pf0-f179.google.com [209.85.192.179])
12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u14JbH5I001618
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 13:37:17 -0600
12, 51 -- Received: by mail-pf0-f179.google.com with SMTP id o185so54699752pfb.1
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 04 Feb 2016 11:37:05 -0800 (PST)
12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=gmail.com; s=20120113;
12, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
12, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 -- b=QsXP/qREJdXB60d+kq9tOKYswsIxOsejc2Xx0ZsSaW6I1xSsgv6VB0IgAa59L3ZPs8
12, 51 -- y9NxWyG8+u6mi4o7fNOPKu8k0MMH6tnxb2/OWLuH6H8OC+Mpdu2mQgLDO2fWLh2zCY27
12, 51 -- xMhSZMh1URCT/r05q6WCAop5f+4NYmK42mCZZJI7L06rRcUpOxVgzd5Jvjr31YcX+iOw
12, 51 -- pgAUshbWLl/es8P8kSsChfHBnivqACxGZJo2rM4JBLWZy4BeFPovRF4LQzgTzvQ/xbI7
12, 51 -- YCT0wrzCA6GQo/mIZCY+bfz3pYrTGhNu84zlBAN0t+h2BamdwaCAge+vTOOuMpY7Zved
12, 51 -- YQEA==
12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=1e100.net; s=20130820;
12, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
12, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 51 -- :content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 -- b=DGs7FDdtbh2/GBMvM/xvhIK/IlfII1jk5Vg+oW8KaH52un8c/A6dYP3JHAO0TshzFz
12, 51 -- +orMNaXTEkO7wBcJFSOiamBz/o8fEIttPmR0SYResk+xhKUVZC7snhCiZntB+98h7GTu
12, 51 -- qw3d6C2SGUYsYtXbiYobyRFgfkqYfURSSBRIz0irpQK4zsfcvbNxIrOLcr8a0rUTsqyG
12, 51 -- rdj3sFXRLe12JA+39UgbSNaz7JUNA4vGUUo1L27UdpInGlspkvN2/DkLPa7DF1xv2+Ei
12, 51 -- 2/D8k6ZMVZFL9lGG1pCMLDD2jwpr8pHgl29JEJxwq/hKvwA60RQr+qGiVtamgMbDiaw/
12, 51 -- SX/A==
12, 51 -- X-Gm-Message-State: AG10YORizZaK0gKgJiORUQAVqV5NMWbq1z3FitfMolDTplrvzZp6q1++r3AJiucEmmrTwg==
12, 51 -- X-Received: by 10.66.150.202 with SMTP id uk10mr13509195pab.73.1454614624629;
12, 51 -- Thu, 04 Feb 2016 11:37:04 -0800 (PST)
12, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
12, 51 -- by smtp.googlemail.com with ESMTPSA id e14sm18982710pap.24.2016.02.04.11.37.02
12, 51 -- for {microscopy-at-microscopy.com}
12, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 51 -- Thu, 04 Feb 2016 11:37:03 -0800 (PST)
12, 51 -- Subject: viaWWW:Speckled TEM Samples
12, 51 -- References: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Reply-To: microscopy.listserver-at-gmail.com
12, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 51 -- X-Forwarded-Message-Id: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Message-ID: {56B3A85C.1030600-at-gmail.com}
12, 51 -- Date: Fri, 5 Feb 2016 06:37:00 +1100
12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
12, 51 -- Gecko/20100101 Thunderbird/38.5.1
12, 51 -- MIME-Version: 1.0
12, 51 -- In-Reply-To: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
12, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: mdelann1-at-jhmi.edu
Date: Thu, 4 Feb 2016 14:08:56 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debra,

This could be a couple of things. First did you use phosphate buffer? If
your concentration was a 100 mM or over, the Ga, osmium and PO4 can
precipitate in the tissue. Check unstained sections to confirm.
Also make sure all the Ga is rinsed out of tissue before you get to osmium.
Are the tissue pieces small? You can also microwave fixative to get better
penetration. Be careful with using reduced osmium and then staining muscle.
Lipid granules are affected with the post-section staining with UA and Pb.
Apparently the lipid material gets lost after staining and rinsing, I have
seen this phenomena and sometimes it will contaminate parts of the section.
If you are sure it isn't your staining protocol, then it must be the
fixation.

Good Luck,
Michael Delannoy

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Sent: Thursday, February 04, 2016 2:50 PM
To: delannoy-at-jhmi.edu

X-from: debrat-at-bcm.edu

This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both debrat-at-bcm.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM
sections. I filter the primary fix mixture with a 0.1um vacuum filtration
unit; I've tried changing the water and am currently using RICCA ultra-pure
H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity
embedding resin. Most recently I have been ethanol-washing all razor blades
used during specimen collection; I use a 0.04% FSG quench after fixation
(didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in
other tissues as well. I just looked at some freshly cut, unstained mouse
foot pad muscle yesterday and there were the black speckles all over the
place! The specks are tiny, much smaller than 10nm. It almost looks like
immuno-gold, except that I haven't done immuno-gold staining in years, and
the specks are not rounded as you might expect with gold particles. They
don't seem to be clustered at any specific organelle, and they are
definitely in the tissue, not on the surface. Someone please help, this is
driving me nuts!

Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from the Microscopy Listserver NO-REPLY
forwarding system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
12, 51 -- From microscopy.listserver-at-gmail.com Thu Feb 4 13:37:17 2016 12,
51 -- Received: from mail-pf0-f179.google.com (mail-pf0-f179.google.com
[209.85.192.179])
12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u14JbH5I001618
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 13:37:17
-0600
12, 51 -- Received: by mail-pf0-f179.google.com with SMTP id
o185so54699752pfb.1
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 04 Feb 2016 11:37:05
-0800 (PST)
12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=gmail.com; s=20120113;
12, 51 --
h=subject:references:reply-to:to:from:message-id:date:user-agent
12, 51 --
:mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 --
b=QsXP/qREJdXB60d+kq9tOKYswsIxOsejc2Xx0ZsSaW6I1xSsgv6VB0IgAa59L3ZPs8
12, 51 --
y9NxWyG8+u6mi4o7fNOPKu8k0MMH6tnxb2/OWLuH6H8OC+Mpdu2mQgLDO2fWLh2zCY27
12, 51 --
xMhSZMh1URCT/r05q6WCAop5f+4NYmK42mCZZJI7L06rRcUpOxVgzd5Jvjr31YcX+iOw
12, 51 --
pgAUshbWLl/es8P8kSsChfHBnivqACxGZJo2rM4JBLWZy4BeFPovRF4LQzgTzvQ/xbI7
12, 51 --
YCT0wrzCA6GQo/mIZCY+bfz3pYrTGhNu84zlBAN0t+h2BamdwaCAge+vTOOuMpY7Zved
12, 51 -- YQEA==
12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=1e100.net; s=20130820;
12, 51 --
h=x-gm-message-state:subject:references:reply-to:to:from:message-id
12, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 51 -- :content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 --
b=DGs7FDdtbh2/GBMvM/xvhIK/IlfII1jk5Vg+oW8KaH52un8c/A6dYP3JHAO0TshzFz
12, 51 --
+orMNaXTEkO7wBcJFSOiamBz/o8fEIttPmR0SYResk+xhKUVZC7snhCiZntB+98h7GTu
12, 51 --
qw3d6C2SGUYsYtXbiYobyRFgfkqYfURSSBRIz0irpQK4zsfcvbNxIrOLcr8a0rUTsqyG
12, 51 --
rdj3sFXRLe12JA+39UgbSNaz7JUNA4vGUUo1L27UdpInGlspkvN2/DkLPa7DF1xv2+Ei
12, 51 --
2/D8k6ZMVZFL9lGG1pCMLDD2jwpr8pHgl29JEJxwq/hKvwA60RQr+qGiVtamgMbDiaw/
12, 51 -- SX/A==
12, 51 -- X-Gm-Message-State:
AG10YORizZaK0gKgJiORUQAVqV5NMWbq1z3FitfMolDTplrvzZp6q1++r3AJiucEmmrTwg==
12, 51 -- X-Received: by 10.66.150.202 with SMTP id
uk10mr13509195pab.73.1454614624629;
12, 51 -- Thu, 04 Feb 2016 11:37:04 -0800 (PST)
12, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
12, 51 -- by smtp.googlemail.com with ESMTPSA id
e14sm18982710pap.24.2016.02.04.11.37.02
12, 51 -- for {microscopy-at-microscopy.com}
12, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 51 -- Thu, 04 Feb 2016 11:37:03 -0800 (PST)
12, 51 -- Subject: viaWWW:Speckled TEM Samples 12, 51 -- References:
{201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Reply-To: microscopy.listserver-at-gmail.com 12, 51 -- To:
MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 51 -- From:
MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 51 --
X-Forwarded-Message-Id: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Message-ID: {56B3A85C.1030600-at-gmail.com} 12, 51 -- Date: Fri, 5
Feb 2016 06:37:00 +1100 12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel
Mac OS X 10.11; rv:38.0) 12, 51 -- Gecko/20100101 Thunderbird/38.5.1 12, 51
-- MIME-Version: 1.0 12, 51 -- In-Reply-To:
{201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12,
51 -- Content-Transfer-Encoding: 7bit ==============================End of -
Headers==============================


==============================Original Headers==============================
21, 23 -- From prvs=835ad12fc=mdelann1-at-jhmi.edu Thu Feb 4 14:08:56 2016
21, 23 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [128.220.39.149])
21, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u14K8tWf023051
21, 23 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 14:08:56 -0600
21, 23 -- X-IronPort-AV: E=Sophos;i="5.22,397,1449550800";
21, 23 -- d="scan'208";a="308084068"
21, 23 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96])
21, 23 -- by IronMTW4.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 04 Feb 2016 15:08:25 -0500
21, 23 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu}
21, 23 -- To: {microscopy.listserver-at-gmail.com} , {delannoy-at-jhmi.edu} ,
21, 23 -- {Microscopy-at-microscopy.com} , {microscopy-at-msa.microscopy.com}
21, 23 -- References: {201602041949.u14JntBl008291-at-ns.microscopy.com}
21, 23 -- In-Reply-To: {201602041949.u14JntBl008291-at-ns.microscopy.com}
21, 23 -- Subject: RE: [Microscopy] viaWWW:Speckled TEM Samples
21, 23 -- Date: Thu, 4 Feb 2016 15:08:22 -0500
21, 23 -- Message-ID: {001601d15f87$cccf33d0$666d9b70$-at-jhmi.edu}
21, 23 -- MIME-Version: 1.0
21, 23 -- Content-Type: text/plain;
21, 23 -- charset="us-ascii"
21, 23 -- Content-Transfer-Encoding: 7bit
21, 23 -- X-Mailer: Microsoft Outlook 14.0
21, 23 -- Thread-Index: AQI3HH9zl4OF9grivIwsXc4fb5E+w55Q1IJQ
21, 23 -- Content-Language: en-us
==============================End of - Headers==============================




From: mdelann1-at-jhmi.edu
Date: Thu, 4 Feb 2016 14:37:08 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Debra,

This could be a couple of things. First did you use phosphate buffer? If
your concentration was a 100 mM or over, the Ga, osmium and PO4 can
precipitate in the tissue. Check unstained sections to confirm.
Also make sure all the Ga is rinsed out of tissue before you get to osmium.
Are the tissue pieces small? You can also microwave fixative to get better
penetration. Be careful with using reduced osmium and then staining muscle.
Lipid granules are affected by the post-section staining with UA and Pb.
Apparently the lipid material gets lost after staining and rinsing, I have
seen this phenomena and sometimes it will contaminate parts of the section.
If you are sure it isn't your staining protocol, then it must be the
fixation.

Good Luck,
Michael Delannoy


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Sent: Thursday, February 04, 2016 2:59 PM
To: mdelann1-at-jhmi.edu

X-from: debrat-at-bcm.edu

This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both debrat-at-bcm.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM
sections. I filter the primary fix mixture with a 0.1um vacuum filtration
unit; I've tried changing the water and am currently using RICCA ultra-pure
H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity
embedding resin. Most recently I have been ethanol-washing all razor blades
used during specimen collection; I use a 0.04% FSG quench after fixation
(didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in
other tissues as well. I just looked at some freshly cut, unstained mouse
foot pad muscle yesterday and there were the black speckles all over the
place! The specks are tiny, much smaller than 10nm. It almost looks like
immuno-gold, except that I haven't done immuno-gold staining in years, and
the specks are not rounded as you might expect with gold particles. They
don't seem to be clustered at any specific organelle, and they are
definitely in the tissue, not on the surface. Someone please help, this is
driving me nuts!

Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from the Microscopy Listserver NO-REPLY
forwarding system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
12, 51 -- From microscopy.listserver-at-gmail.com Thu Feb 4 13:37:17 2016 12,
51 -- Received: from mail-pf0-f179.google.com (mail-pf0-f179.google.com
[209.85.192.179])
12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u14JbH5I001618
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 13:37:17
-0600
12, 51 -- Received: by mail-pf0-f179.google.com with SMTP id
o185so54699752pfb.1
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 04 Feb 2016 11:37:05
-0800 (PST)
12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=gmail.com; s=20120113;
12, 51 --
h=subject:references:reply-to:to:from:message-id:date:user-agent
12, 51 --
:mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 --
b=QsXP/qREJdXB60d+kq9tOKYswsIxOsejc2Xx0ZsSaW6I1xSsgv6VB0IgAa59L3ZPs8
12, 51 --
y9NxWyG8+u6mi4o7fNOPKu8k0MMH6tnxb2/OWLuH6H8OC+Mpdu2mQgLDO2fWLh2zCY27
12, 51 --
xMhSZMh1URCT/r05q6WCAop5f+4NYmK42mCZZJI7L06rRcUpOxVgzd5Jvjr31YcX+iOw
12, 51 --
pgAUshbWLl/es8P8kSsChfHBnivqACxGZJo2rM4JBLWZy4BeFPovRF4LQzgTzvQ/xbI7
12, 51 --
YCT0wrzCA6GQo/mIZCY+bfz3pYrTGhNu84zlBAN0t+h2BamdwaCAge+vTOOuMpY7Zved
12, 51 -- YQEA==
12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=1e100.net; s=20130820;
12, 51 --
h=x-gm-message-state:subject:references:reply-to:to:from:message-id
12, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 51 -- :content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 --
b=DGs7FDdtbh2/GBMvM/xvhIK/IlfII1jk5Vg+oW8KaH52un8c/A6dYP3JHAO0TshzFz
12, 51 --
+orMNaXTEkO7wBcJFSOiamBz/o8fEIttPmR0SYResk+xhKUVZC7snhCiZntB+98h7GTu
12, 51 --
qw3d6C2SGUYsYtXbiYobyRFgfkqYfURSSBRIz0irpQK4zsfcvbNxIrOLcr8a0rUTsqyG
12, 51 --
rdj3sFXRLe12JA+39UgbSNaz7JUNA4vGUUo1L27UdpInGlspkvN2/DkLPa7DF1xv2+Ei
12, 51 --
2/D8k6ZMVZFL9lGG1pCMLDD2jwpr8pHgl29JEJxwq/hKvwA60RQr+qGiVtamgMbDiaw/
12, 51 -- SX/A==
12, 51 -- X-Gm-Message-State:
AG10YORizZaK0gKgJiORUQAVqV5NMWbq1z3FitfMolDTplrvzZp6q1++r3AJiucEmmrTwg==
12, 51 -- X-Received: by 10.66.150.202 with SMTP id
uk10mr13509195pab.73.1454614624629;
12, 51 -- Thu, 04 Feb 2016 11:37:04 -0800 (PST)
12, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
12, 51 -- by smtp.googlemail.com with ESMTPSA id
e14sm18982710pap.24.2016.02.04.11.37.02
12, 51 -- for {microscopy-at-microscopy.com}
12, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 51 -- Thu, 04 Feb 2016 11:37:03 -0800 (PST)
12, 51 -- Subject: viaWWW:Speckled TEM Samples 12, 51 -- References:
{201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Reply-To: microscopy.listserver-at-gmail.com 12, 51 -- To:
MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 51 -- From:
MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 51 --
X-Forwarded-Message-Id: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Message-ID: {56B3A85C.1030600-at-gmail.com} 12, 51 -- Date: Fri, 5
Feb 2016 06:37:00 +1100 12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel
Mac OS X 10.11; rv:38.0) 12, 51 -- Gecko/20100101 Thunderbird/38.5.1 12, 51
-- MIME-Version: 1.0 12, 51 -- In-Reply-To:
{201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12,
51 -- Content-Transfer-Encoding: 7bit ==============================End of -
Headers==============================


==============================Original Headers==============================
22, 22 -- From prvs=835ad12fc=mdelann1-at-jhmi.edu Thu Feb 4 14:37:08 2016
22, 22 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [162.129.8.200])
22, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u14Kb8uR011842
22, 22 -- for {Microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 14:37:08 -0600
22, 22 -- X-IronPort-AV: E=Sophos;i="5.22,397,1449550800";
22, 22 -- d="scan'208";a="148194861"
22, 22 -- Received: from unknown (HELO BSNO8925) ([10.16.66.96])
22, 22 -- by IPEB3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 04 Feb 2016 15:36:55 -0500
22, 22 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu}
22, 22 -- To: {Microscopy-at-microscopy.com}
22, 22 -- References: {201602041959.u14JxNM9016455-at-ns.microscopy.com}
22, 22 -- In-Reply-To: {201602041959.u14JxNM9016455-at-ns.microscopy.com}
22, 22 -- Subject: RE: [Microscopy] viaWWW:Speckled TEM Samples
22, 22 -- Date: Thu, 4 Feb 2016 15:36:53 -0500
22, 22 -- Message-ID: {000b01d15f8b$c85128f0$58f37ad0$-at-jhmi.edu}
22, 22 -- MIME-Version: 1.0
22, 22 -- Content-Type: text/plain;
22, 22 -- charset="us-ascii"
22, 22 -- Content-Transfer-Encoding: 7bit
22, 22 -- X-Mailer: Microsoft Outlook 14.0
22, 22 -- Thread-Index: AQHW27qbS6YBhbTIxFfmYXtaaKJibJ8RX0Xw
22, 22 -- Content-Language: en-us
==============================End of - Headers==============================




From: wij.muss-at-aon.at
Date: Fri, 5 Feb 2016 04:40:28 -0600
Subject: [Microscopy] Re: Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Debra,

thank you for your thorough listing of your processing step...
what you omit, what you changed, etc., etc...
Nevertheless you ought to tell us about the
== buffer vehicle in fixation as well as after fixation
== osmication data
== processing schedule right after osmium into ascending alcohols,
the latter most important to know which concentration you start.
Could it be that you face "salt & pepper" artifact?
It would be interesting to see an image of your speckles...

Best regards,
Wolfgang

Wolfgang MUSS, PhD
Member of MSA until 2015
Retired by Dec. 1st, 2015
SALZBURG, AUSTRIA
wij.muss-at-aon.at



-----Urspr,ngliche Nachricht-----
Von: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Gesendet: Donnerstag, 4. Februar 2016 21:05
An: wij.muss-at-aon.at
Betreff: [Microscopy] Speckled TEM Samples

----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

This is awful - I am having a terrible time getting clean TEM sections.
I filter the primary fix mixture with a 0.1um vacuum filtration unit;
I've tried changing the water and am currently using RICCA ultra-pure H2O;
I have stopped en bloc staining altogether;
I use Spurr's Low Viscosity embedding resin.
Most recently I have been ethanol-washing all razor blades used during
specimen collection;
I use a 0.04% FSG quench after fixation (didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in
other tissues as well. I just looked at some freshly cut, unstained mouse
foot pad muscle yesterday and there were the black speckles all over the
place! The specks are tiny, much smaller than 10nm. It almost looks like
immuno-gold, except that I haven't done immuno-gold staining in years, and
the specks are not rounded as you might expect with gold particles.
They don't seem to be clustered at any specific organelle, and they are
definitely in the tissue, not on the surface. Someone please help, this is
driving me nuts!

Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------
--
===============================================
Do not reply to this messages it is from the Microscopy Listserver NO-REPLY
forwarding system. You should send a new message to
Microscopy-at-Microscopy.com
=============================================
==============================Original Headers==============================
12, 51 -- From microscopy.listserver-at-gmail.com Thu Feb 4 13:37:17 2016 12,
51 -- Received: from mail-pf0-f179.google.com (mail-pf0-f179.google.com
[209.85.192.179])
12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u14JbH5I001618
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 13:37:17
-0600
12, 51 -- Received: by mail-pf0-f179.google.com with SMTP id
o185so54699752pfb.1
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 04 Feb 2016 11:37:05
-0800 (PST)
12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=gmail.com; s=20120113;
12, 51 --
h=subject:references:reply-to:to:from:message-id:date:user-agent
12, 51 --
:mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 --
b=QsXP/qREJdXB60d+kq9tOKYswsIxOsejc2Xx0ZsSaW6I1xSsgv6VB0IgAa59L3ZPs8
12, 51 --
y9NxWyG8+u6mi4o7fNOPKu8k0MMH6tnxb2/OWLuH6H8OC+Mpdu2mQgLDO2fWLh2zCY27
12, 51 --
xMhSZMh1URCT/r05q6WCAop5f+4NYmK42mCZZJI7L06rRcUpOxVgzd5Jvjr31YcX+iOw
12, 51 --
pgAUshbWLl/es8P8kSsChfHBnivqACxGZJo2rM4JBLWZy4BeFPovRF4LQzgTzvQ/xbI7
12, 51 --
YCT0wrzCA6GQo/mIZCY+bfz3pYrTGhNu84zlBAN0t+h2BamdwaCAge+vTOOuMpY7Zved
12, 51 -- YQEA==
12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=1e100.net; s=20130820;
12, 51 --
h=x-gm-message-state:subject:references:reply-to:to:from:message-id
12, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 51 -- :content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 --
b=DGs7FDdtbh2/GBMvM/xvhIK/IlfII1jk5Vg+oW8KaH52un8c/A6dYP3JHAO0TshzFz
12, 51 --
+orMNaXTEkO7wBcJFSOiamBz/o8fEIttPmR0SYResk+xhKUVZC7snhCiZntB+98h7GTu
12, 51 --
qw3d6C2SGUYsYtXbiYobyRFgfkqYfURSSBRIz0irpQK4zsfcvbNxIrOLcr8a0rUTsqyG
12, 51 --
rdj3sFXRLe12JA+39UgbSNaz7JUNA4vGUUo1L27UdpInGlspkvN2/DkLPa7DF1xv2+Ei
12, 51 --
2/D8k6ZMVZFL9lGG1pCMLDD2jwpr8pHgl29JEJxwq/hKvwA60RQr+qGiVtamgMbDiaw/
12, 51 -- SX/A==
12, 51 -- X-Gm-Message-State:
AG10YORizZaK0gKgJiORUQAVqV5NMWbq1z3FitfMolDTplrvzZp6q1++r3AJiucEmmrTwg==
12, 51 -- X-Received: by 10.66.150.202 with SMTP id
uk10mr13509195pab.73.1454614624629;
12, 51 -- Thu, 04 Feb 2016 11:37:04 -0800 (PST)
12, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
12, 51 -- by smtp.googlemail.com with ESMTPSA id
e14sm18982710pap.24.2016.02.04.11.37.02
12, 51 -- for {microscopy-at-microscopy.com}
12, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 51 -- Thu, 04 Feb 2016 11:37:03 -0800 (PST)
12, 51 -- Subject: viaWWW:Speckled TEM Samples 12, 51 -- References:
{201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Reply-To: microscopy.listserver-at-gmail.com 12, 51 -- To:
MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 51 -- From:
MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 51 --
X-Forwarded-Message-Id: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Message-ID: {56B3A85C.1030600-at-gmail.com} 12, 51 -- Date: Fri, 5
Feb 2016 06:37:00 +1100 12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel
Mac OS X 10.11; rv:38.0) 12, 51 -- Gecko/20100101 Thunderbird/38.5.1 12, 51
-- MIME-Version: 1.0 12, 51 -- In-Reply-To:
{201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12,
51 -- Content-Transfer-Encoding: 7bit ==============================End of -
Headers==============================




==============================Original Headers==============================
13, 23 -- From wij.muss-at-aon.at Fri Feb 5 04:40:26 2016
13, 23 -- Received: from bsmtp8.bon.at (bsmtp8.bon.at [213.33.87.20])
13, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15AeL6M022046
13, 23 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 04:40:21 -0600
13, 23 -- Received: from anwender1976b3 (unknown [93.83.25.98])
13, 23 -- by bsmtp8.bon.at (Postfix) with ESMTPA id 3pxYD82BkDz5tlR;
13, 23 -- Fri, 5 Feb 2016 11:40:08 +0100 (CET)
13, 23 -- From: "Wolfgang Muss" {wij.muss-at-aon.at}
13, 23 -- To: {debrat-at-bcm.edu}
13, 23 -- Cc: {microscopy-at-microscopy.com}
13, 23 -- References: {201602042005.u14K5SeB022620-at-ns.microscopy.com}
13, 23 -- In-Reply-To: {201602042005.u14K5SeB022620-at-ns.microscopy.com}
13, 23 -- Subject: [Microscopy] Re: Speckled TEM Samples
13, 23 -- Date: Fri, 5 Feb 2016 11:40:59 +0100
13, 23 -- Message-ID: {004201d16001$b3cd8740$1b6895c0$-at-muss-at-aon.at}
13, 23 -- MIME-Version: 1.0
13, 23 -- Content-Type: text/plain;
13, 23 -- charset="iso-8859-1"
13, 23 -- X-Mailer: Microsoft Office Outlook 12.0
13, 23 -- Thread-Index: AdFfqKW5Na3c1P5rRhWKa2WMh1An2wAV/XRw
13, 23 -- Content-Language: de
13, 23 -- Content-Transfer-Encoding: 8bit
13, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15AeL6M022046
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 06:04:14 -0600
Subject: [Microscopy] viaWWW:Image of speckled mouse muscle.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: debrat-at-bcm.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both debrat-at-bcm.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: debrat-at-bcm.edu
Name: bandic00t

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM sections

Message: http://s450.photobucket.com/user/wassupdoc1/library/Science

Image of speckled mouse muscle.

Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------




--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
15, 51 -- From microscopy.listserver-at-gmail.com Fri Feb 5 06:04:11 2016
15, 51 -- Received: from mail-pa0-f53.google.com (mail-pa0-f53.google.com [209.85.220.53])
15, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15C47k8001768
15, 51 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 06:04:08 -0600
15, 51 -- Received: by mail-pa0-f53.google.com with SMTP id cy9so33393683pac.0
15, 51 -- for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 04:03:55 -0800 (PST)
15, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=gmail.com; s=20120113;
15, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
15, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 51 -- bh=Pd6UoYj2G1dBxnk9+TeYG5E7pnpFPQ0UuhB7svTgG3g=;
15, 51 -- b=cOJjlNaoIjt6pgBDOMs5Z96bPrIDwUURu/2hM/7x31ZDwR5Z//ywLqP2HwJAVx7an7
15, 51 -- GLUZS+/2h725UjxkwjzE61h+/UHpd8dXKdW08TN9ilL6TYqOFukZLwNnlp+6335EBfbE
15, 51 -- /TOMKj0clGfbIITb8OWQ4UcMUQXb+ob6yuj4englv8UIuxX3H9esO4eQcis+5zYg/p3P
15, 51 -- wyoyH6c4ArQ5iHUz/1Rp3yNEsD0cEyaxqTYTF76jIOtOvCdnmW/5wSdA3tp449ENLrAK
15, 51 -- F+MgiFLFP2c+/sDpwJiFWSdycs77rJvMiIzVnCXO3rY35J7P9JcgpLCFhzJz8ES0fp+T
15, 51 -- j/LQ==
15, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=1e100.net; s=20130820;
15, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
15, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
15, 51 -- :content-transfer-encoding;
15, 51 -- bh=Pd6UoYj2G1dBxnk9+TeYG5E7pnpFPQ0UuhB7svTgG3g=;
15, 51 -- b=JxlCYkwWsNfbWhXM8C2YRhdysaaxIkJd2mdY4UK0jKc061agLrPgqGvzo2XtXE9AgH
15, 51 -- 0aaaIe30Ay0QqOS3sF/bU42AWtfV/tDLYDK2AwnEDlvDUnymR2F2SptTaO6CazQxwBzd
15, 51 -- QJH1jIkXcwnZThEj+/jVfKJe9QZjX57bmTi+HI/aFGBBbM3oevh+K64qNm+MSQNYuSjl
15, 51 -- 6jwb2ri+3p8r+fti/I21JonXgVZg2YOkQ9pes6nB7SBs7O2OcSUiNm4H748qV5d2jBmv
15, 51 -- zCjvXgNCt1gxiJlc490cj8bI6z4QCdU2xt8Jwr8+BJZ7nPr1SP38hNAuNTHNLMKBsMoQ
15, 51 -- EJ/w==
15, 51 -- X-Gm-Message-State: AG10YOTdw8LhqTTrlcb7zPaImf40DtN+WgzWL+qHZv1Vh6eHlTyfJR/5rxwigk12Bg25TA==
15, 51 -- X-Received: by 10.67.3.170 with SMTP id bx10mr19318792pad.34.1454673835223;
15, 51 -- Fri, 05 Feb 2016 04:03:55 -0800 (PST)
15, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
15, 51 -- by smtp.googlemail.com with ESMTPSA id t29sm24177174pfi.8.2016.02.05.04.03.53
15, 51 -- for {microscopy-at-microscopy.com}
15, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 51 -- Fri, 05 Feb 2016 04:03:54 -0800 (PST)
15, 51 -- Subject: viaWWW:Image of speckled mouse muscle.
15, 51 -- References: {201602042138.u14LcKgN001323-at-ns.microscopy.com}
15, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 51 -- Reply-To: microscopy.listserver-at-gmail.com
15, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 51 -- X-Forwarded-Message-Id: {201602042138.u14LcKgN001323-at-ns.microscopy.com}
15, 51 -- Message-ID: {56B48FA7.9090405-at-gmail.com}
15, 51 -- Date: Fri, 5 Feb 2016 23:03:51 +1100
15, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
15, 51 -- Gecko/20100101 Thunderbird/38.5.1
15, 51 -- MIME-Version: 1.0
15, 51 -- In-Reply-To: {201602042138.u14LcKgN001323-at-ns.microscopy.com}
15, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
15, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Fri, 5 Feb 2016 07:23:55 -0600
Subject: [Microscopy] RE: viaWWW:Image of speckled mouse muscle.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Debra,
Thank you for showing us the image with "speckles". I think that the problem might be in lead staining procedure. Please, look at Arvid B. Maunsbach & Bj/drn A. Afzelius "Biomedical Electron Microscopy (Illustrated Methods and Interpretations)" 1999, Pages 207,Aei228 {doi:10.1016/B978-012480610-8/50011-1} for explanation. We had also similar problems with lead carbonate precipitations in the past. Since our technician started to wear a face shield during sections staining with lead citrate the precipitates (contamination) in them almost vanished.

There is a old paper on "How to remove such precipitates from ultrathin sections":
Kuo, J. (1980), A simple method for removing stain precipitates from biological sections for transmission electron microscopy. Journal of Microscopy, 120: 221,Aei224. {doi:10.1111/j.1365-2818.1980.tb04140.x}

We tried this approach several times, but the results were not ideal in our hands.


Best regards

Oldrich

--
Oldrich Benada
Institute of Microbiology CAS, v.v.i.
Videnska 1083
142 20 Prague 4
Czech Republic

On Fri, 5 Feb 2016 06:29:23 -0600, microscopy.listserver-at-gmail.com wrote :
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} X-from: debrat-at-bcm.edu
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
} http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both debrat-at-bcm.edu as well as the Microscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: debrat-at-bcm.edu
} Name: bandic00t
}
} Organization: Baylor College of Medicine
}
} Title-Subject: [Filtered] Speckled TEM sections
}
} Message: http://s450.photobucket.com/user/wassupdoc1/library/Science
}
} Image of speckled mouse muscle.
}
} Login Host: 128.249.1.198
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
}
}
} --
} ===============================================
}
} Do not reply to this messages it is from
} the Microscopy Listserver NO-REPLY forwarding
} system. You should send a new message to
}
} Microscopy-at-Microscopy.com
}
} =============================================
}
} ==============================Original
} Headers============================== 15, 51 -- From
} microscopy.listserver-at-gmail.com Fri Feb 5 06:04:11 2016 15, 51 --
} Received: from mail-pa0-f53.google.com (mail-pa0-f53.google.com
} [209.85.220.53]) 15, 51 -- by ns.microscopy.com
} (8.12.11.20060308/8.12.8) with ESMTP id u15C47k8001768 15, 51 --
} for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 06:04:08
} -0600 15, 51 -- Received: by mail-pa0-f53.google.com with SMTP id
} cy9so33393683pac.0 15, 51 -- for {microscopy-at-microscopy.com} ;
} Fri, 05 Feb 2016 04:03:55 -0800 (PST) 15, 51 -- DKIM-Signature: v=1;
} a=rsa-sha256; c=relaxed/relaxed; 15, 51 -- d=gmail.com;
} s=20120113; 15, 51 --
} h=subject:references:to:reply-to:from:message-id:date:user-agent 15,
} 51
} -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
} 15, 51 -- bh=Pd6UoYj2G1dBxnk9+TeYG5E7pnpFPQ0UuhB7svTgG3g=;
} 15, 51 --
} b=cOJjlNaoIjt6pgBDOMs5Z96bPrIDwUURu/2hM/7x31ZDwR5Z//ywLqP2HwJAVx7an7
} 15, 51 --
} GLUZS+/2h725UjxkwjzE61h+/UHpd8dXKdW08TN9ilL6TYqOFukZLwNnlp+6335EBfbE
} 15, 51
} -- /TOMKj0clGfbIITb8OWQ4UcMUQXb+ob6yuj4englv8UIuxX3H9esO4eQcis+5zYg/p3P
} 15, 51 --
} wyoyH6c4ArQ5iHUz/1Rp3yNEsD0cEyaxqTYTF76jIOtOvCdnmW/5wSdA3tp449ENLrAK
} 15, 51 --
} F+MgiFLFP2c+/sDpwJiFWSdycs77rJvMiIzVnCXO3rY35J7P9JcgpLCFhzJz8ES0fp+T
} 15, 51 -- j/LQ== 15, 51 -- X-Google-DKIM-Signature: v=1;
} a=rsa-sha256; c=relaxed/relaxed; 15, 51 -- d=1e100.net;
} s=20130820; 15, 51 --
} h=x-gm-message-state:subject:references:to:reply-to:from:message-id
} 15, 51
} -- :date:user-agent:mime-version:in-reply-to:content-type
} 15, 51 -- :content-transfer-encoding; 15, 51 --
} bh=Pd6UoYj2G1dBxnk9+TeYG5E7pnpFPQ0UuhB7svTgG3g=; 15, 51 --
} b=JxlCYkwWsNfbWhXM8C2YRhdysaaxIkJd2mdY4UK0jKc061agLrPgqGvzo2XtXE9AgH
} 15, 51 --
} 0aaaIe30Ay0QqOS3sF/bU42AWtfV/tDLYDK2AwnEDlvDUnymR2F2SptTaO6CazQxwBzd
} 15, 51 --
} QJH1jIkXcwnZThEj+/jVfKJe9QZjX57bmTi+HI/aFGBBbM3oevh+K64qNm+MSQNYuSjl
} 15, 51 --
} 6jwb2ri+3p8r+fti/I21JonXgVZg2YOkQ9pes6nB7SBs7O2OcSUiNm4H748qV5d2jBmv
} 15, 51 --
} zCjvXgNCt1gxiJlc490cj8bI6z4QCdU2xt8Jwr8+BJZ7nPr1SP38hNAuNTHNLMKBsMoQ
} 15, 51 -- EJ/w== 15, 51 -- X-Gm-Message-State:
} AG10YOTdw8LhqTTrlcb7zPaImf40DtN+WgzWL+qHZv1Vh6eHlTyfJR/5rxwigk12Bg25TA==
} 15, 51 -- X-Received: by 10.67.3.170 with SMTP id
} bx10mr19318792pad.34.1454673835223; 15, 51 -- Fri, 05 Feb
} 2016 04:03:55 -0800 (PST) 15, 51 -- Received: from
} Nestors-MacBookAir-Pro-2014.local ([119.17.52.122]) 15, 51 --
} by smtp.googlemail.com with ESMTPSA id
} t29sm24177174pfi.8.2016.02.05.04.03.53 15, 51 -- for
} {microscopy-at-microscopy.com} 15, 51 -- (version=TLSv1/SSLv3
} cipher=OTHER); 15, 51 -- Fri, 05 Feb 2016 04:03:54 -0800
} (PST) 15, 51 -- Subject: viaWWW:Image of speckled mouse muscle. 15,
} 51 -- References: {201602042138.u14LcKgN001323-at-ns.microscopy.com} 15,
} 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
} 15, 51 -- Reply-To: microscopy.listserver-at-gmail.com 15, 51 -- From:
} MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 15, 51
} -- X-Forwarded-Message-Id:
} {201602042138.u14LcKgN001323-at-ns.microscopy.com} 15, 51 -- Message-ID:
} {56B48FA7.9090405-at-gmail.com} 15, 51 -- Date: Fri, 5 Feb 2016 23:03:51
} +1100 15, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X
} 10.11; rv:38.0) 15, 51 -- Gecko/20100101 Thunderbird/38.5.1 15, 51
} -- MIME-Version: 1.0 15, 51 -- In-Reply-To:
} {201602042138.u14LcKgN001323-at-ns.microscopy.com} 15, 51 --
} Content-Type: text/plain; charset=windows-1252; format=flowed 15, 51
} -- Content-Transfer-Encoding: 7bit ==============================End
} of - Headers==============================



Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.



==============================Original Headers==============================
13, 45 -- From benada-at-biomed.cas.cz Fri Feb 5 07:23:53 2016
13, 45 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11])
13, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15DNptA021087
13, 45 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 07:23:52 -0600
13, 45 -- X-ASG-Debug-ID: 1454678618-05011e54256712a0001-4CH8be
13, 45 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id inb1JH2j2MV5h0Ez (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 14:23:38 +0100 (CET)
13, 45 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz
13, 45 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32]
13, 45 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32
13, 45 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12])
13, 45 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
13, 45 -- (No client certificate requested)
13, 45 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 388CF2C8070
13, 45 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 14:23:33 +0100 (CET)
13, 45 -- Date: Fri, 5 Feb 2016 14:23:32 +0100
13, 45 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
13, 45 -- To: {microscopy-at-microscopy.com}
13, 45 -- Subject: RE: [Microscopy] viaWWW:Image of speckled mouse muscle.
13, 45 -- Message-ID: {20160205142332.74306056-at-u117ob02}
13, 45 -- X-ASG-Orig-Subj: RE: [Microscopy] viaWWW:Image of speckled mouse muscle.
13, 45 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV
13, 45 -- =?UTF-8?B?xIxS?=
13, 45 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu)
13, 45 -- MIME-Version: 1.0
13, 45 -- Content-Type: text/plain; charset=UTF-8
13, 45 -- X-IoP-CAS-MailScanner-ID: 388CF2C8070.773A9
13, 45 -- X-IoP-CAS-MailScanner: Processed
13, 45 -- X-Spam-Status: No
13, 45 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32]
13, 45 -- X-Barracuda-Start-Time: 1454678618
13, 45 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA
13, 45 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi
13, 45 -- X-Barracuda-Scan-Msg-Size: 7668
13, 45 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz
13, 45 -- X-Barracuda-BRTS-Status: 1
13, 45 -- X-Barracuda-Spam-Score: 1.00
13, 45 -- X-Barracuda-Spam-Status: No, SCORE=1.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_RULE_7582B
13, 45 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.26769
13, 45 -- Rule breakdown below
13, 45 -- pts rule name description
13, 45 -- ---- ---------------------- --------------------------------------------------
13, 45 -- 0.50 BSF_RULE7568M Custom Rule 7568M
13, 45 -- 0.50 BSF_RULE_7582B Custom Rule 7582B
13, 45 -- Content-Transfer-Encoding: 8bit
13, 45 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15DNptA021087
==============================End of - Headers==============================




From: nsa2-at-leicester.ac.uk
Date: Fri, 5 Feb 2016 10:37:09 -0600
Subject: [Microscopy] viaWWW:Speckled TEM Samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Debra,

Difficult to say when we don't know your primary buffer / fixation protocol, but I would agree that it looks like it may be due to either precipitate from a phosphate buffer, possibly due to insufficient washing between Ga/OSO4 steps, or osmium 'peppering', usually produced with buffer reactions in the osmium steps, or prolonged fixation in osmium.

I used to have similar problems, and resolved them by changing my buffer to HEPES or cacodylate, extra wash steps between the glutaraldehyde and osmium fixes, and secondary fixing in aqueous osmium as opposed to buffered osmium.

Good luck resolving it - let us know how you get on!

Natalie


Miss Natalie Allcock
EM Facility Manager

Electron Microscopy Facility,
Centre for Core Biotechnology Services,
University of Leicester, University Road, Leicester, LE1 7RH, UK
*
t: +44 (0)116 2523370
e:* nsa2-at-leicester.ac.uk
w: http://www2.le.ac.uk/colleges/medbiopsych/research/cbs/eml/electron-microscopy

Follow us on Twitter or visit our Facebook page


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Sent: 04 February 2016 20:05
To: Allcock, Natalie S.

X-from: debrat-at-bcm.edu

This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both debrat-at-bcm.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: debrat-at-bcm.edu
Name: Debra M Townley

Organization: Baylor College of Medicine

Title-Subject: [Filtered] Speckled TEM Samples

Message: This is awful - I am having a terrible time getting clean TEM sections. I filter the primary fix mixture with a 0.1um vacuum filtration unit; I've tried changing the water and am currently using RICCA ultra-pure H2O; I have stopped en bloc staining altogether; I use Spurr's Low Viscosity embedding resin. Most recently I have been ethanol-washing all razor blades used during specimen collection; I use a 0.04% FSG quench after fixation (didn't seem to make a difference).
The fine speckling is most noticeable in muscle tissue, although I see it in other tissues as well. I just looked at some freshly cut, unstained mouse foot pad muscle yesterday and there were the black speckles all over the place! The specks are tiny, much smaller than 10nm. It almost looks like immuno-gold, except that I haven't done immuno-gold staining in years, and the specks are not rounded as you might expect with gold particles. They don't seem to be clustered at any specific organelle, and they are definitely in the tissue, not on the surface. Someone please help, this is driving me nuts!

Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from the Microscopy Listserver NO-REPLY forwarding system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
12, 51 -- From microscopy.listserver-at-gmail.com Thu Feb 4 13:37:17 2016 12, 51 -- Received: from mail-pf0-f179.google.com (mail-pf0-f179.google.com [209.85.192.179])
12, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u14JbH5I001618
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 4 Feb 2016 13:37:17 -0600
12, 51 -- Received: by mail-pf0-f179.google.com with SMTP id o185so54699752pfb.1
12, 51 -- for {microscopy-at-microscopy.com} ; Thu, 04 Feb 2016 11:37:05 -0800 (PST)
12, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=gmail.com; s=20120113;
12, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
12, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 -- b=QsXP/qREJdXB60d+kq9tOKYswsIxOsejc2Xx0ZsSaW6I1xSsgv6VB0IgAa59L3ZPs8
12, 51 -- y9NxWyG8+u6mi4o7fNOPKu8k0MMH6tnxb2/OWLuH6H8OC+Mpdu2mQgLDO2fWLh2zCY27
12, 51 -- xMhSZMh1URCT/r05q6WCAop5f+4NYmK42mCZZJI7L06rRcUpOxVgzd5Jvjr31YcX+iOw
12, 51 -- pgAUshbWLl/es8P8kSsChfHBnivqACxGZJo2rM4JBLWZy4BeFPovRF4LQzgTzvQ/xbI7
12, 51 -- YCT0wrzCA6GQo/mIZCY+bfz3pYrTGhNu84zlBAN0t+h2BamdwaCAge+vTOOuMpY7Zved
12, 51 -- YQEA==
12, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 51 -- d=1e100.net; s=20130820;
12, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
12, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 51 -- :content-transfer-encoding;
12, 51 -- bh=c5TNmviJYdtIBItMhLaglCcUQVjb8lLoOgPVFhKBi+4=;
12, 51 -- b=DGs7FDdtbh2/GBMvM/xvhIK/IlfII1jk5Vg+oW8KaH52un8c/A6dYP3JHAO0TshzFz
12, 51 -- +orMNaXTEkO7wBcJFSOiamBz/o8fEIttPmR0SYResk+xhKUVZC7snhCiZntB+98h7GTu
12, 51 -- qw3d6C2SGUYsYtXbiYobyRFgfkqYfURSSBRIz0irpQK4zsfcvbNxIrOLcr8a0rUTsqyG
12, 51 -- rdj3sFXRLe12JA+39UgbSNaz7JUNA4vGUUo1L27UdpInGlspkvN2/DkLPa7DF1xv2+Ei
12, 51 -- 2/D8k6ZMVZFL9lGG1pCMLDD2jwpr8pHgl29JEJxwq/hKvwA60RQr+qGiVtamgMbDiaw/
12, 51 -- SX/A==
12, 51 -- X-Gm-Message-State: AG10YORizZaK0gKgJiORUQAVqV5NMWbq1z3FitfMolDTplrvzZp6q1++r3AJiucEmmrTwg==
12, 51 -- X-Received: by 10.66.150.202 with SMTP id uk10mr13509195pab.73.1454614624629;
12, 51 -- Thu, 04 Feb 2016 11:37:04 -0800 (PST)
12, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
12, 51 -- by smtp.googlemail.com with ESMTPSA id e14sm18982710pap.24.2016.02.04.11.37.02
12, 51 -- for {microscopy-at-microscopy.com}
12, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 51 -- Thu, 04 Feb 2016 11:37:03 -0800 (PST)
12, 51 -- Subject: viaWWW:Speckled TEM Samples 12, 51 -- References: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Reply-To: microscopy.listserver-at-gmail.com 12, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com} 12, 51 -- X-Forwarded-Message-Id: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Message-ID: {56B3A85C.1030600-at-gmail.com} 12, 51 -- Date: Fri, 5 Feb 2016 06:37:00 +1100 12, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0) 12, 51 -- Gecko/20100101 Thunderbird/38.5.1 12, 51 -- MIME-Version: 1.0 12, 51 -- In-Reply-To: {201602041737.u14Hb1rV031472-at-ns.microscopy.com}
12, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 51 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================


==============================Original Headers==============================
28, 32 -- From nsa2-at-leicester.ac.uk Fri Feb 5 10:37:08 2016
28, 32 -- Received: from springbok.le.ac.uk (springbok.le.ac.uk [143.210.4.39])
28, 32 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15Gb7XO026437
28, 32 -- for {Microscopy-at-Microscopy.com} ; Fri, 5 Feb 2016 10:37:08 -0600
28, 32 -- Received: from exp-casht-i1.le.ac.uk ([143.210.133.193]:60984 helo=exp-casht-i1.uol.le.ac.uk)
28, 32 -- by springbok.le.ac.uk with esmtps (TLS1.0:RSA_AES_256_CBC_SHA1:256)
28, 32 -- (Exim 4.80)
28, 32 -- (envelope-from {nsa2-at-leicester.ac.uk} )
28, 32 -- id 1aRjNP-0002Zn-9k
28, 32 -- for Microscopy-at-Microscopy.com; Fri, 05 Feb 2016 16:36:55 +0000
28, 32 -- Received: from EXP-DAG1-N2.uol.le.ac.uk ([::1]) by exp-casht-i1.uol.le.ac.uk
28, 32 -- ([143.210.133.193]) with mapi id 14.03.0235.001; Fri, 5 Feb 2016 16:36:54
28, 32 -- +0000
28, 32 -- From: "Allcock, Natalie S." {nsa2-at-leicester.ac.uk}
28, 32 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com}
28, 32 -- Subject: RE: [Microscopy] viaWWW:Speckled TEM Samples
28, 32 -- Thread-Topic: [Microscopy] viaWWW:Speckled TEM Samples
28, 32 -- Thread-Index: AQHRX4djr+BZgXyAZUWLxR7GGRpol58dphzQ
28, 32 -- Date: Fri, 5 Feb 2016 16:36:53 +0000
28, 32 -- Message-ID: {C31045C47F49444BA76584B3DC6020CE16D97457-at-exp-dag1-n2.uol.le.ac.uk}
28, 32 -- References: {201602042005.u14K5QQr022575-at-ns.microscopy.com}
28, 32 -- In-Reply-To: {201602042005.u14K5QQr022575-at-ns.microscopy.com}
28, 32 -- Accept-Language: en-GB, en-US
28, 32 -- Content-Language: en-US
28, 32 -- X-MS-Has-Attach:
28, 32 -- X-MS-TNEF-Correlator:
28, 32 -- x-originating-ip: [143.210.155.106]
28, 32 -- Content-Type: text/plain; charset="iso-8859-1"
28, 32 -- MIME-Version: 1.0
28, 32 -- X-UoL-Id: 9c031989f04d052606a0482156e2d3bd-at-1aRjNP-0002Zn-9k-at-springbok.le.ac.uk
28, 32 -- Content-Transfer-Encoding: 8bit
28, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u15Gb7XO026437
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 13:56:32 -0600
Subject: [Microscopy] viaWWW:Leitz Aristoplan microscope detailled service manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jmhermel-at-inaf.cnrs-gif.fr

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both jmhermel-at-inaf.cnrs-gif.fr as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: jmhermel-at-inaf.cnrs-gif.fr
Name: Hermel

Organization: CNRS

Title-Subject: [Filtered] Leitz Aristoplan microscope detailled service
manual

Message: Hi,
I should appreciate any help or link to find a Leitz Aristoplan
microscope detailled service manual. This microscope has a problem with
de centering of lenses in the microscope stand. Thank you very much.

Login Host: 157.136.59.144
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
11, 51 -- From microscopy.listserver-at-gmail.com Fri Feb 5 13:56:31 2016
11, 51 -- Received: from mail-pa0-f52.google.com (mail-pa0-f52.google.com [209.85.220.52])
11, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15JuVTU018972
11, 51 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 13:56:31 -0600
11, 51 -- Received: by mail-pa0-f52.google.com with SMTP id ho8so39027741pac.2
11, 51 -- for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 11:56:19 -0800 (PST)
11, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 51 -- d=gmail.com; s=20120113;
11, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
11, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
11, 51 -- bh=5capA/b6iIfMiS7s2pXIRXu73agj24QODDRiWO0TCkE=;
11, 51 -- b=mb69nnSBtEK7xmJGU2ypp6jdee/bGOrQ+ihxMndMNxHuUXX3vm8tlhB0WdCCsdM5pv
11, 51 -- +pUNwTK08+igaw79bz281uE/BtndCttYHEAEJzoXF0o0t4SJ+zCOhKPnrtwfpFThm0/P
11, 51 -- EKCBStnGw6qeOqjdwCmFbMCISSDl7frzYFYel6qS5fLRjaJUkp4+UfACEupKNGstvEas
11, 51 -- KLYWlMWlSLqHAaFyOLf6GeRNkh8AJFZpPwugszrf6epvirwnsTtiz7OuzzJdNC/8NQ9R
11, 51 -- FEjeBWg/w1brdnjlWLW5XJWsLDbKPCgIIuCtJrm3AlZlw5qYkv/h8vQSzWfH4fcnNWy0
11, 51 -- HIVg==
11, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 51 -- d=1e100.net; s=20130820;
11, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
11, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
11, 51 -- :content-transfer-encoding;
11, 51 -- bh=5capA/b6iIfMiS7s2pXIRXu73agj24QODDRiWO0TCkE=;
11, 51 -- b=K8DZNd8o/xG6esWX6vEA6qCkGDM3n36sNiWdJDBa2UWkk7l1gzlgGBacCAajfvHBpK
11, 51 -- Z8P6iL94l3E+jh7payaFvSIYVcvhn3f0gg4SI8+JMgdINfOk38mqYWfGuCbnU5gGZdr/
11, 51 -- +50SrHv9XEmWdYHJ7TSOTOxad5oXkaYFUAeA2rwlExVKexv7Muu7vsSLHZRnfc1wAleN
11, 51 -- 36IyVo5Wtp1fZUwmrRDJfSXfo3dDjmdn0nGMH296+lcvZePfnk00DpAy2/rggl46WPiR
11, 51 -- WJjLwoE8ZRaQ0rpj8VgA8vErffP9u5XKInzm3smrOaxE8hnWcS6hg9m9tWr5uiSozQHR
11, 51 -- bFHQ==
11, 51 -- X-Gm-Message-State: AG10YORF3Rym36+i92iY9w2BLoHK9MMtVQFcLXFQaxshs/rVv24S+N1NbqaoePulecJOYg==
11, 51 -- X-Received: by 10.66.227.133 with SMTP id sa5mr22483282pac.36.1454702178699;
11, 51 -- Fri, 05 Feb 2016 11:56:18 -0800 (PST)
11, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
11, 51 -- by smtp.googlemail.com with ESMTPSA id o73sm26341132pfa.37.2016.02.05.11.56.16
11, 51 -- for {microscopy-at-microscopy.com}
11, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
11, 51 -- Fri, 05 Feb 2016 11:56:18 -0800 (PST)
11, 51 -- Subject: viaWWW:Leitz Aristoplan microscope detailled service manual
11, 51 -- References: {201602051256.u15Cuj4S014609-at-ns.microscopy.com}
11, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
11, 51 -- Reply-To: microscopy.listserver-at-gmail.com
11, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
11, 51 -- X-Forwarded-Message-Id: {201602051256.u15Cuj4S014609-at-ns.microscopy.com}
11, 51 -- Message-ID: {56B4FE5E.2000809-at-gmail.com}
11, 51 -- Date: Sat, 6 Feb 2016 06:56:14 +1100
11, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
11, 51 -- Gecko/20100101 Thunderbird/38.5.1
11, 51 -- MIME-Version: 1.0
11, 51 -- In-Reply-To: {201602051256.u15Cuj4S014609-at-ns.microscopy.com}
11, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 13:58:42 -0600
Subject: [Microscopy] viaWWW:Open EM Position at the Molecular Foundry

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: mlibbee-at-lbl.gov

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both mlibbee-at-lbl.gov as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: mlibbee-at-lbl.gov
Name: Marissa Libbee

Organization: LBL

Title-Subject: [Filtered] Open Position at the Molecular Foundry

Message: See description below. For more information, please follow the
link http://jobs.lbl.gov/open-positions.html


Molecular Foundry Research Scientist-81646
Organization:MS-Materials Sciences
Description

Berkeley Lab is Bringing Science Solutions to the World, and YOU can be
a part of it!

In the world of science, Lawrence Berkeley National Laboratory (Berkeley
Lab) is synonymous with "excellence." That's why we hire the best -
whether in research, finance or other operations. This is a great
opportunity to bring your top-notch skills to bear in support of
world-class scientific research that addresses national and global
challenges!

Position Summary:
The National Center for Electron Microscopy (NCEM) facility in the
Molecular Foundry at Lawrence Berkeley National Laboratory is seeking a
Research Scientist to establish an internationally recognized theory and
image simulation research program to advance the state of the art in
electron microscopy. The primary responsibilities are: 1) to develop
theory and methodology for quantitative comparison between theoretical
models and data from advanced electron microscopy instruments, and 2) to
lead the development of new techniques and instrumentation for electron
scattering. This position will require working on a wide range of
collaborative research projects in materials characterization and
establishment of an outstanding user program and individual research
program.

What You Will Do:
Develop image processing and simulation tools to enhance capabilities
for quantitative data analysis.
Develop an independent research program. Lead or participate in the
development of new research directions.
Offer computational support for ultrahigh resolution microscopy in
aberration-corrected instruments in TEM and STEM. Devise new approaches
to overcoming limitations of exit wave reconstruction routines and
implement these approaches in NCEM-is user program. Refine theory and
simulation for STEM imaging to quantify image analysis.
Contribute creatively to the development of techniques for atomic
resolution tomography.
Establish protocols and work flows for the effective management of large
data sets for in-house and remote analysis
Participate in the design and implementation of new techniques for
imaging of energy-related materials, including enhanced visibility and
analysis of light elements, imaging at low dose, analysis of defects,
and imaging of thick samples.
Provide theoretical insight and support as part of a team to develop new
detectors, holography, and other instrumentation and techniques.
Lead an effort to help close the gap between experimentally available
data and theoretically accessible systems.
Be familiar with materials modeling and computational procedures for
direct comparison with experimental observations. Modeling approaches
could range from macroscopic to atomistic methods, from elastic or
thermodynamic to kinetic or first principles techniques.
Serve as point of contact for the theory and simulation requirements of
users related to nanoscale phenomena accessible to electron microscopy
imaging. Oversee operation of image analysis and computer lab at NCEM.
Provide expertise in the application of existing commercial software and
the development of custom modules and scripts.
Serve as scientific contact for user proposals. Critically evaluate
scientific proposals for feasibility; design research plans and
appropriate microscopy experiments to accomplish specific research
objectives. Improve quality of the analysis component of user projects
by providing theory and simulation support and collaboration.
Work closely with other Molecular Foundry staff scientists.
Disseminate research results through publications and presentations at
national and international conferences.
What Is Required:
Proven ability to develop and apply computational methods for image
analysis, image simulation and advanced codes for modeling of materials
behavior.
Excellent scientific publications record in relevant areas
Well-developed oral and written communication skills. Ability to work
effectively within a team and with various levels of internal and
external users and staff.
Experience in the management and manipulation of large data sets
commensurate with high resolution electron microscopy
Ph.D. or equivalent experience in the Physical Sciences or Engineering
Strong organizational and time management skills. Ability to manage
competing priorities, provide quality work within tight time
constraints, and deliver projects on schedule.
Additional Desired Qualifications:
Experience with computational materials modeling such as density
functional theory, molecular dynamics, etc.
Strong background of work with experimental data and analysis of images
from in situ experiments.

Notes: This is a career appointment.

PLEASE NOTE: This job will close Feb 7, at midnight.

This position requires completion of a background check.

Berkeley Lab addresses the world-is most urgent scientific challenges by
advancing sustainable energy, protecting human health, creating new
materials, and revealing the origin and fate of the universe. Founded in
1931, Berkeley Lab-is scientific expertise has been recognized with 13
Nobel prizes. The University of California manages Berkeley Lab for the
U.S. Department of Energy-is Office of Science.

Equal Employment Opportunity: Berkeley Lab is an Equal
Opportunity/Affirmative Action Employer. All qualified applicants will
receive consideration for employment without regard to race, color,
religion, sex, sexual orientation, gender identity, national origin,
disability, age, or protected veteran status. Berkeley Lab is in
compliance with the Pay Transparency Nondiscrimination Provision under
41 CFR 60-1.4. Click here to view the poster and supplement: "Equal
Employment Opportunity is the Law."

Login Host: 131.243.3.252
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
24, 51 -- From microscopy.listserver-at-gmail.com Fri Feb 5 13:58:40 2016
24, 51 -- Received: from mail-pa0-f45.google.com (mail-pa0-f45.google.com [209.85.220.45])
24, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15JwdLj019272
24, 51 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 13:58:39 -0600
24, 51 -- Received: by mail-pa0-f45.google.com with SMTP id yy13so39001952pab.3
24, 51 -- for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 11:58:27 -0800 (PST)
24, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
24, 51 -- d=gmail.com; s=20120113;
24, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
24, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
24, 51 -- bh=r/C0ff3k/ptai6F04RjQIujC/XMLKH/hvQiY6Q9pBas=;
24, 51 -- b=OR/4qB6nlzMSnmYwYb5XLDyCLyRZUDC7SNFeOu+psua4peD6gaXzjejeV666adTls9
24, 51 -- PY2I5FzHeilUJ9DxhbwKvBaSOsskUXEOhFyFLwRz+ma6JgCKBLOnGoyWuDw3H+Xwk51i
24, 51 -- DXqrCEj6Y2YoJKjkr/9eQU8GV+LHe3t/p+c+LyczzYJn8pdMabBJAjkei8fOmP+ntL/i
24, 51 -- l3eEE+b0Z/NPVz8MUoeXiLYgAgFLdXjK8xkUq/0KadYLfkXUo03YX4V3828+a8HUEuwl
24, 51 -- bBzVxiPP8LLPFs8akUL9KMtxRNvh1foDSau6dC1R56aGozOZSF/YmQmeZS17iDcSLTpH
24, 51 -- ZsNw==
24, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
24, 51 -- d=1e100.net; s=20130820;
24, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
24, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
24, 51 -- :content-transfer-encoding;
24, 51 -- bh=r/C0ff3k/ptai6F04RjQIujC/XMLKH/hvQiY6Q9pBas=;
24, 51 -- b=RhpbtgxlLdIrb4lXrRqCHf7H6I6BzEodeTo1hze00nH7ZvvyURQ6ses7uaGcCvqZbq
24, 51 -- Jt5dd4f88EsRZpTMq0/ssvLcUIpM6jOunZbTr1U2dWw0Wlddy2UIGoPo2oRjg3YLL4p+
24, 51 -- stcOtVECFG9ikd3BEpfFkMtrLdDdMOufJ3p4qzrjJOOzxGUPxKDqdCNPQ+KweXAQhfU0
24, 51 -- pBAnz5hHbY7WBHG2mxZc9rusv441NKBnFvqsb5dMopHfKN7l7DhiFm21oznaXfZQWKMJ
24, 51 -- +K4kRDRuLE98JHAxZkQnrRBjyR4ZoUmn02EVD8Sqq8kQwngCGUe758y/OUdLXbsrbLQ5
24, 51 -- 31Pg==
24, 51 -- X-Gm-Message-State: AG10YOS5WjFSO3SrxlmjrShFcyKFjHakSqXxHsLHfcH0CJmvIO7Tfo7X8Qz8JdXEfRZN6g==
24, 51 -- X-Received: by 10.66.236.103 with SMTP id ut7mr22423174pac.4.1454702306768;
24, 51 -- Fri, 05 Feb 2016 11:58:26 -0800 (PST)
24, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
24, 51 -- by smtp.googlemail.com with ESMTPSA id s21sm7651184pfi.29.2016.02.05.11.58.25
24, 51 -- for {microscopy-at-microscopy.com}
24, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
24, 51 -- Fri, 05 Feb 2016 11:58:26 -0800 (PST)
24, 51 -- Subject: viaWWW:Open EM Position at the Molecular Foundry
24, 51 -- References: {201602051844.u15IipV3017502-at-ns.microscopy.com}
24, 51 -- Reply-To: microscopy.listserver-at-gmail.com
24, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
24, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
24, 51 -- X-Forwarded-Message-Id: {201602051844.u15IipV3017502-at-ns.microscopy.com}
24, 51 -- Message-ID: {56B4FEDF.1000808-at-gmail.com}
24, 51 -- Date: Sat, 6 Feb 2016 06:58:23 +1100
24, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
24, 51 -- Gecko/20100101 Thunderbird/38.5.1
24, 51 -- MIME-Version: 1.0
24, 51 -- In-Reply-To: {201602051844.u15IipV3017502-at-ns.microscopy.com}
24, 51 -- Content-Type: text/plain; charset=UTF-8; format=flowed
24, 51 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Feb 2016 13:59:44 -0600
Subject: [Microscopy] viaWWW:Research and Development Scientist UK Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jhyun-at-gatan.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both jhyun-at-gatan.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] Research and Development Scientist UK Job Opening

Message: Location: Abingdon, Oxfordshire UK

Gatan is an industry leader in the electron microscopy industry and the
Research & Development Scientist will work as part of multidisciplinary
teams of scientists and development engineers. The role will provide
technical input and deliver innovative technology to new product
introductions, meeting project schedules and budgets. There will be a
requirement for collaboration across all business functions working
closely with the Marketing, Manufacturing and Customer Service teams to
assure the success of all new product introductions. The building of
relationships and working closely with internal and external customers
will be a key requirement.

The prime responsibility will be to engage in product development tasks
according to Gatan-is product life cycle (PLC) model, a phased process
that includes concept and feasibility, design and development, alpha and
beta pilot phases leading to product release. Other responsibilities
will involve sustaining engineering for mature and legacy products along
with the provision of engineering support to Gatan-is production and
customer service teams. The product range and technical challenges are
diverse and so the ideal candidate will have a broad range of scientific
and engineering skills.

The successful candidate will be cable of working semi-autonomously on
multiple tasks and must be capable of providing innovative solutions to
technical challenges in high technology product design and development.
Equally important will be a keen eye for cost effective solutions that
are designed for test, manufacture and service.
The role will report directly to the Director of Engineering (UK) and
some UK and international travel will be required.

For a full description and application instructions, visit:
http://www.gatan.com/company/careers/research-and-development-scientist



Login Host: 50.58.128.11
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
17, 51 -- From microscopy.listserver-at-gmail.com Fri Feb 5 13:59:39 2016
17, 51 -- Received: from mail-pa0-f52.google.com (mail-pa0-f52.google.com [209.85.220.52])
17, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u15JxbbV019472
17, 51 -- for {microscopy-at-microscopy.com} ; Fri, 5 Feb 2016 13:59:37 -0600
17, 51 -- Received: by mail-pa0-f52.google.com with SMTP id uo6so39747578pac.1
17, 51 -- for {microscopy-at-microscopy.com} ; Fri, 05 Feb 2016 11:59:25 -0800 (PST)
17, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 51 -- d=gmail.com; s=20120113;
17, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
17, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
17, 51 -- bh=JaQTYjCkkPd90qwawb2V14MI9a32oZs5z8XUk58e6z4=;
17, 51 -- b=JfIewSjHaoT1peb4Mv6MCAw2HynzmTJ3AOGPDMUlbHPtLW2EHadW5SC5Muy7z3FgON
17, 51 -- o++bxx98YCZay/6GH0++bZbaVxWMBcps83qBTrWjRZaef2zu6Vdanej+OrQuAw5aitga
17, 51 -- scxM5rKMnYXkoj9raU/o7hQb63JxUAW+8/7wFwqNJRKVb74OydHGVV7ltLOoU7iES0Ln
17, 51 -- ly0BiZNxoJxvuGflfsIhPQWBe6oW0uT/b39uNbqSOIBpEDNg+Ts4vNIN1UTR0k8sD4hz
17, 51 -- 7H3Xy83hFTSMIcdqVaJw6qx5ksW0fx7ktxzA0rmUREkopvG0XQzdfTKE/QmjtUTFOOrZ
17, 51 -- 12jQ==
17, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 51 -- d=1e100.net; s=20130820;
17, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
17, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
17, 51 -- :content-transfer-encoding;
17, 51 -- bh=JaQTYjCkkPd90qwawb2V14MI9a32oZs5z8XUk58e6z4=;
17, 51 -- b=b3daw8eaEEKiGppoEKQDZWSz7z8O/KFVlvtApQz8c3RBYAoNEPxyrHSlpBi3nfCPxu
17, 51 -- LFzGvin3yjgcDbnbRjlnnn6VcYFLfPVnM/R3gkFZrmrsk7Gu9C1WBReMu7imocKh4Cwc
17, 51 -- DPrxEOzOLHvqiU7WjEEeILIBIFb8YLoCtGBk8mLKgddd6HWx6qx1D2iaYcXJ3BLFnQMR
17, 51 -- wRfgi/HLAHrxw8nTHWs0hXcwlLW88L2r9sOdF/ni1xzVGt/0H2/hMoC5Gu5UermfcVkI
17, 51 -- yWLbqLnViS0THjI3JNZKN+lXQpMZ60U24Ik1lB+dBYso8ZK3kq7gH9sfl8rXNOkSdmz3
17, 51 -- oBCg==
17, 51 -- X-Gm-Message-State: AG10YOS1sDYcyYWNa+ciiHkOQYrXHoXpfM5ZaV3NrI6r2LvyiSWkWzja9EdaAR652By9mw==
17, 51 -- X-Received: by 10.66.150.66 with SMTP id ug2mr22864176pab.114.1454702364888;
17, 51 -- Fri, 05 Feb 2016 11:59:24 -0800 (PST)
17, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([119.17.52.122])
17, 51 -- by smtp.googlemail.com with ESMTPSA id qj8sm26247423pac.40.2016.02.05.11.59.23
17, 51 -- for {microscopy-at-microscopy.com}
17, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 51 -- Fri, 05 Feb 2016 11:59:24 -0800 (PST)
17, 51 -- Subject: viaWWW:Research and Development Scientist UK Job Opening
17, 51 -- References: {201602051900.u15J08Jg018017-at-ns.microscopy.com}
17, 51 -- Reply-To: microscopy.listserver-at-gmail.com
17, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 51 -- X-Forwarded-Message-Id: {201602051900.u15J08Jg018017-at-ns.microscopy.com}
17, 51 -- Message-ID: {56B4FF19.5080304-at-gmail.com}
17, 51 -- Date: Sat, 6 Feb 2016 06:59:21 +1100
17, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 51 -- Gecko/20100101 Thunderbird/38.5.1
17, 51 -- MIME-Version: 1.0
17, 51 -- In-Reply-To: {201602051900.u15J08Jg018017-at-ns.microscopy.com}
17, 51 -- Content-Type: text/plain; charset=UTF-8; format=flowed
17, 51 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================






From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Feb 2016 14:38:17 -0600
Subject: [Microscopy] viaWWW:Oxford INCA documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: opmills-at-mtu.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both opmills-at-mtu.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: opmills-at-mtu.edu
Name: Owen Mills

Organization: Michigan Tech University

Title-Subject: [Filtered] Oxford INCA documentation

Message: Hello

We hope someone on the list has a copy (paper or electronic) of the
Oxford INCA User Manual. We were given an 80 page INCA Operator Manual
document that provides only a cursory explanation of topics like
managing the master standards database. For comparison the Aztec manual
is 500 pages. Maybe what we are seeking doesn't exist, but if you do
have a manual we'd be happy to pay copying or shipping costs.

Any help you can supply would be appreciated.

Cheers
Owen
--
Owen P Mills
Director, Applied Chemical and Morphological Analysis Laboratory
Michigan Technological University
1400 Townsend Dr
Houghton, MI 49931
906-369-1875
opmills-at-mtu.edu
http://mcff.mtu.edu/acmal/

Login Host: 141.219.30.140
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
15, 51 -- From microscopy.listserver-at-gmail.com Mon Feb 8 14:38:14 2016
15, 51 -- Received: from mail-pf0-f178.google.com (mail-pf0-f178.google.com [209.85.192.178])
15, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18KcDLL020641
15, 51 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 14:38:13 -0600
15, 51 -- Received: by mail-pf0-f178.google.com with SMTP id q63so11383488pfb.0
15, 51 -- for {microscopy-at-microscopy.com} ; Mon, 08 Feb 2016 12:38:00 -0800 (PST)
15, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=gmail.com; s=20120113;
15, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
15, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 51 -- bh=QZCWlmx+/IADQLZvmj8uObL9f0IqCK/x406c76hA9k8=;
15, 51 -- b=bcbxavUwitRjK7DTyPyiCpFBST7VJ7Fz6JPqSRiufJUyjcOOX5l9EZjFg+y5v9yQce
15, 51 -- QaekR0M1D1KiN1ms36Lq9Bvm/sTzDsZNsY1p4AvG+f3hm8lVg7Mm91Br5S54Lp6JrJSd
15, 51 -- Z/ikJg34QD1kpe5b0JVL0lBrBumyfZXE24+Vry5OT+tcmPOvHLJgtYHscE4uW7kw4n+H
15, 51 -- Lm3Msa7PtYxUZOF//tZrDmXYM6jw6jXzVso/SQcYl0WyCU4cRvFEG8ALvxrQpoTPe1sG
15, 51 -- igTMXU5su0sGukUE0Q3ShF2MOsUqaQHbPFnEmB36FvgK1Mid9oOIaeiVa5HmQM/FR+ay
15, 51 -- kIpw==
15, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=1e100.net; s=20130820;
15, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
15, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
15, 51 -- :content-transfer-encoding;
15, 51 -- bh=QZCWlmx+/IADQLZvmj8uObL9f0IqCK/x406c76hA9k8=;
15, 51 -- b=gN3+AF0TLVs4IJHWXAQHLoAOa2+Md8V03Z0wF29YGLBLNiomhS20WrIiUsz9no/HNZ
15, 51 -- 0ZbuGdOEygFs5YKWelePsq9EdY8CaDSDqf2BPZwX6kgrWoEalgZYtXAsegHTM+WRtklW
15, 51 -- fbF2oi91hZiLNKPNVg+kLpGxAGx0P5kIVfHOTRpt2AX+1AXtdaE3myfVdfZe6gKllUEM
15, 51 -- pgYUsAU2bs6rk/zLIBMxnzff65XQEaBZSqQHrVQArlbdx+xcRguke8D5MCQulF1q95BO
15, 51 -- ljAY1aQR10f0UV45Tquh4huNCy9qBnYKNs67ladiVsMKf8346IGKD3qDFHOw1ieCPZgF
15, 51 -- Cxqw==
15, 51 -- X-Gm-Message-State: AG10YORwqfXm9XQSBjd7wadXedOMT8lz4G4bgb9Cd3alZge3HuYALje8GN2KbD2RWfWZbA==
15, 51 -- X-Received: by 10.98.31.21 with SMTP id f21mr32788158pff.134.1454963880328;
15, 51 -- Mon, 08 Feb 2016 12:38:00 -0800 (PST)
15, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([59.167.247.4])
15, 51 -- by smtp.googlemail.com with ESMTPSA id z3sm45414488par.17.2016.02.08.12.37.58
15, 51 -- for {microscopy-at-microscopy.com}
15, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 51 -- Mon, 08 Feb 2016 12:37:59 -0800 (PST)
15, 51 -- Subject: viaWWW:Oxford INCA documentation
15, 51 -- References: {201602081321.u18DLoR2004248-at-ns.microscopy.com}
15, 51 -- Reply-To: microscopy.listserver-at-gmail.com
15, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 51 -- X-Forwarded-Message-Id: {201602081321.u18DLoR2004248-at-ns.microscopy.com}
15, 51 -- Message-ID: {56B8FCA4.8070803-at-gmail.com}
15, 51 -- Date: Tue, 9 Feb 2016 07:37:56 +1100
15, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
15, 51 -- Gecko/20100101 Thunderbird/38.5.1
15, 51 -- MIME-Version: 1.0
15, 51 -- In-Reply-To: {201602081321.u18DLoR2004248-at-ns.microscopy.com}
15, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
15, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Feb 2016 14:39:06 -0600
Subject: [Microscopy] viaWWW:TEM - Cryo-ultramicrotomy of rubber sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: badamianand-at-bfusa.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both badamianand-at-bfusa.com as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: badamianand-at-bfusa.com
Name: Anand Badami

Organization: Bridgestone Research

Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections

Message: Can anyone recommend a technique for collecting rubber sections
during cryo-ultramicrotomy that enables the sections to lie flat (i.e.
as wrinkle free as possible) on a TEM support grid? I currently collect
my sections off the back of my cryoknife using a sucrose droplet, but as
the sections warm coming out of the cryochamber on the droplet they
contract and wrinkle before being deposited on a TEM grid (with or
without carbon coating). Chamber temperature is -120 C. Any ideas are
appreciated. Thank you.

Login Host: 199.48.24.10
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
11, 51 -- From microscopy.listserver-at-gmail.com Mon Feb 8 14:39:06 2016
11, 51 -- Received: from mail-pa0-f44.google.com (mail-pa0-f44.google.com [209.85.220.44])
11, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18Kd5Gi020785
11, 51 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 14:39:06 -0600
11, 51 -- Received: by mail-pa0-f44.google.com with SMTP id uo6so80304577pac.1
11, 51 -- for {microscopy-at-microscopy.com} ; Mon, 08 Feb 2016 12:38:53 -0800 (PST)
11, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 51 -- d=gmail.com; s=20120113;
11, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
11, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
11, 51 -- bh=Kd5wPa3xL1KRy21HGzZ7X/1KILdCjQGptbL380mN9oo=;
11, 51 -- b=tthUUgpnwEQ+/+FXvI4g+6//GD35VIG65lSudPBHHCZpJF1Zpq8Qt1Mrebo6bmcn37
11, 51 -- xwSjCr5lu+VcwFJ0XHfYIGwSHGovcOlJ0Oh47oEVopf8p+MTg8ogCJTPSe4QI4esa+AJ
11, 51 -- fPVud3b56AAiM4wGwXiobGS60+ZbeP1ojP71ouqZwnsSKtpkze+NjYR10Uzt39MouYZn
11, 51 -- 6vHZ/Ret95BoMeDP/C6cslY18KqDGeGePG1qxJMgR4wk/VpomMzD7qL9v1zx00DTPcAL
11, 51 -- BVhGdB245vFuSEOq4F2HD580/i+FD9IsimEoT3f/3Q49P0fU33XMp5xvFlm1laiBWOMo
11, 51 -- N+Fw==
11, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 51 -- d=1e100.net; s=20130820;
11, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
11, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
11, 51 -- :content-transfer-encoding;
11, 51 -- bh=Kd5wPa3xL1KRy21HGzZ7X/1KILdCjQGptbL380mN9oo=;
11, 51 -- b=ZAyLks47+lo2nsw2/whVVR8XiysNYlUQYhqwOmtEce4RVxOGomUL73+XTEs7DcaIfz
11, 51 -- 2uqpT+/4bVdaoQvaQAFGWdwV8u+QWxCr7yL9IYX0l8MEXljvUf5aCq9ZXtgG1j6tpErV
11, 51 -- T8aooEl4YPMMMsPLoeYaCw/F6OPg9oE/bgq4uGhCwiQEfxIC0xj6cWfBeanCHDZ/J9wl
11, 51 -- LiyKb5eziLytHxxttunij9L4tiNBYsH7xcrl2LSI1XZk7q4WrHCLAQD+PzMJid/sevla
11, 51 -- s/KUw82LQcefvP89VyZuP2XSsamQL01WueIezEGw+dlvoncnBTNjIrn0+I6PLuXEIdxR
11, 51 -- IKzg==
11, 51 -- X-Gm-Message-State: AG10YOTf/EYZc9TwROKoW3gLvAdNzbyO86Ti///deXL4qiIEMwIvykzVi++4beLc2F2R1A==
11, 51 -- X-Received: by 10.66.147.136 with SMTP id tk8mr45253166pab.157.1454963932939;
11, 51 -- Mon, 08 Feb 2016 12:38:52 -0800 (PST)
11, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([59.167.247.4])
11, 51 -- by smtp.googlemail.com with ESMTPSA id u5sm13127576pfi.15.2016.02.08.12.38.51
11, 51 -- for {microscopy-at-microscopy.com}
11, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
11, 51 -- Mon, 08 Feb 2016 12:38:52 -0800 (PST)
11, 51 -- Subject: viaWWW:TEM - Cryo-ultramicrotomy of rubber sections
11, 51 -- References: {201602081713.u18HD135013683-at-ns.microscopy.com}
11, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
11, 51 -- Reply-To: microscopy.listserver-at-gmail.com
11, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
11, 51 -- X-Forwarded-Message-Id: {201602081713.u18HD135013683-at-ns.microscopy.com}
11, 51 -- Message-ID: {56B8FCD9.5000703-at-gmail.com}
11, 51 -- Date: Tue, 9 Feb 2016 07:38:49 +1100
11, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
11, 51 -- Gecko/20100101 Thunderbird/38.5.1
11, 51 -- MIME-Version: 1.0
11, 51 -- In-Reply-To: {201602081713.u18HD135013683-at-ns.microscopy.com}
11, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Feb 2016 14:45:08 -0600
Subject: [Microscopy] viaWWW:Upcoming AFM/SPM courses in US, Europe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: dalia.yablon-at-surfacechar.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both dalia.yablon-at-surfacechar.com as well as the
Microscopy Listserver
---------------------------------------------------------------------------

Email: dalia.yablon-at-surfacechar.com
Name: Dalia Yablon

Organization: SurfaceChar

Title-Subject: [Filtered] Upcoming AFM/SPM courses in US, Europe

Message: Dear colleagues,

There are several scanning probe microscopy/atomic force microscopy
courses that are happening this spring in both the US and Europe:

April 5-7, 2016 Netherlands: Overview of Scanning Probe Microscopy: A
hands-on short course covering the technology, basics of operation, and
applications. This course has a March 5, 2016 registration deadline.
For course details and registration, go to
http://www.surfacechar.com/classesschedule.html

May 10-11, 2016 Boston MA: AFM for Characterization of Polymer
Materials. A 2-day hands on course focusing on AFM for polymer
applications to study polymer structure, morphology, and materials
properties. For course details and registration, go to
http://www.afmworkshop.com/characterization-of-polymer-materials-afm-training.html

May 22, 2016 Washington DC: Nanoscale Materials Characterization for
the Biomedical and Pharmaceutical Industry. For course details and
registration, go to http://techconnectworld.com/World2016/workshops/518.html

Sincerely,

Dalia Yablon, Ph.D.
SurfaceChar LLC
www.surfacechar.com
Dalia.yablon-at-surfacechar.com


Login Host: 65.96.10.44
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
21, 51 -- From microscopy.listserver-at-gmail.com Mon Feb 8 14:45:07 2016
21, 51 -- Received: from mail-pa0-f44.google.com (mail-pa0-f44.google.com [209.85.220.44])
21, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18Kj6YM022266
21, 51 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 14:45:06 -0600
21, 51 -- Received: by mail-pa0-f44.google.com with SMTP id ho8so79055565pac.2
21, 51 -- for {microscopy-at-microscopy.com} ; Mon, 08 Feb 2016 12:44:53 -0800 (PST)
21, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
21, 51 -- d=gmail.com; s=20120113;
21, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
21, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
21, 51 -- bh=DTQka575bdI5uDjp5oTt00hT0NwoeZAS/lzD9O+ryqU=;
21, 51 -- b=BrYGeEa76M/vjqnV55aGkSOSCikDveplEHwu6pE5Z2I7c5L9+bBt4I1WwyKq4ZaByF
21, 51 -- VguMqouAg4VH6FSlcrtTgMWZf0I/cz8QbOuDIcv949mfH7yveIzDmVTMuMZxMFFjBST5
21, 51 -- 973qIO1sAl9kQ9l/8JRnjiPCwPvP+c1vsYVBOqXHmE1jIIsNnAXHmz8YhAOXp85jLM7o
21, 51 -- DPKt5DNO9MLX2V0OSF085vKK8yAuUxYTuoicgzSIw+PKgUQfMlrL0qyztQpmM6bJdLcO
21, 51 -- nXV24B+nvK3FJpgkRbyri7eZsh69UeA5eMxs5lTTO3pT877xB+dsIYsJDNV45r8WK1Ol
21, 51 -- m4Vw==
21, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
21, 51 -- d=1e100.net; s=20130820;
21, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
21, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
21, 51 -- :content-transfer-encoding;
21, 51 -- bh=DTQka575bdI5uDjp5oTt00hT0NwoeZAS/lzD9O+ryqU=;
21, 51 -- b=WbTH4J+bGjR6DitDquMn9S+bs17alxJmFORjOO88xhXsM5W2lD/LaZZwaAzObFA1xw
21, 51 -- TjWpur79GHfH/HmB6xri6QB3+NloJZm6SVk8XbYRgQDWs6lWQe2kKhcCenFhVTbu08R3
21, 51 -- eTsAhhqnRqFWdkPCnUGhLcN47fzs7KkYBDh9+i0jbMRybixQ1vur0nFKJXXqFFNFj7qT
21, 51 -- OrLgsLKVcmNBNowImZrMjP1BEKB7vQ9qkhiQy8+EHfGoB5eynb6tIXNjTnpyD2XTArG+
21, 51 -- E1T2WY519UubeHs4MdmhtV53RWIx9urYUMylYKxU0GvQuK1I4u3o81Zr+IisI16dsSKA
21, 51 -- sgog==
21, 51 -- X-Gm-Message-State: AG10YORpku+LplN+dg3Y5z218EOx/dVRr6XWMTIcjzx+ItXFA9QbA0s2aTMNLDPmqvenzQ==
21, 51 -- X-Received: by 10.66.222.101 with SMTP id ql5mr45229913pac.144.1454964293588;
21, 51 -- Mon, 08 Feb 2016 12:44:53 -0800 (PST)
21, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([59.167.247.4])
21, 51 -- by smtp.googlemail.com with ESMTPSA id n4sm45462162pfi.3.2016.02.08.12.44.52
21, 51 -- for {microscopy-at-microscopy.com}
21, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
21, 51 -- Mon, 08 Feb 2016 12:44:53 -0800 (PST)
21, 51 -- Subject: viaWWW:Upcoming AFM/SPM courses in US, Europe
21, 51 -- References: {201602082039.u18Kdc7u020905-at-ns.microscopy.com}
21, 51 -- Reply-To: microscopy.listserver-at-gmail.com
21, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
21, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
21, 51 -- X-Forwarded-Message-Id: {201602082039.u18Kdc7u020905-at-ns.microscopy.com}
21, 51 -- Message-ID: {56B8FE42.40900-at-gmail.com}
21, 51 -- Date: Tue, 9 Feb 2016 07:44:50 +1100
21, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
21, 51 -- Gecko/20100101 Thunderbird/38.5.1
21, 51 -- MIME-Version: 1.0
21, 51 -- In-Reply-To: {201602082039.u18Kdc7u020905-at-ns.microscopy.com}
21, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
21, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: leunissen-at-aurion.nl
Date: Mon, 8 Feb 2016 16:06:47 -0600
Subject: [Microscopy] Re: viaWWW:TEM - Cryo-ultramicrotomy of rubber sections

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good morning Anand,

if the wrinkling happens during warming of the droplet, could it be because of the section surface characteristics?
Adding salt to the sucrose should help if the surface is charged, multivalent ion salts would be more suited than monovalent.
However if the surface is not charged but hydrophobic adding salt would probably increase the wrinkling, and in that case adding a hydrophobic component might help, such as serum albumin. Some detergents can do both.

Good luck!


Jan Leunissen
Dunedin, NZ

} On 9/02/2016, at 09:41, microscopy.listserver-at-gmail.com wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} X-from: badamianand-at-bfusa.com
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both badamianand-at-bfusa.com as well as the Microscopy
} Listserver
} ---------------------------------------------------------------------------
}
} Email: badamianand-at-bfusa.com
} Name: Anand Badami
}
} Organization: Bridgestone Research
}
} Title-Subject: [Filtered] TEM - Cryo-ultramicrotomy of rubber sections
}
} Message: Can anyone recommend a technique for collecting rubber sections
} during cryo-ultramicrotomy that enables the sections to lie flat (i.e.
} as wrinkle free as possible) on a TEM support grid? I currently collect
} my sections off the back of my cryoknife using a sucrose droplet, but as
} the sections warm coming out of the cryochamber on the droplet they
} contract and wrinkle before being deposited on a TEM grid (with or
} without carbon coating). Chamber temperature is -120 C. Any ideas are
} appreciated. Thank you.
}
} Login Host: 199.48.24.10
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
} --
} ===============================================
}
} Do not reply to this messages it is from
} the Microscopy Listserver NO-REPLY forwarding
} system. You should send a new message to
}
} Microscopy-at-Microscopy.com
}
} =============================================
}
} ==============================Original Headers==============================
} 11, 51 -- From microscopy.listserver-at-gmail.com Mon Feb 8 14:39:06 2016
} 11, 51 -- Received: from mail-pa0-f44.google.com (mail-pa0-f44.google.com [209.85.220.44])
} 11, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18Kd5Gi020785
} 11, 51 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 14:39:06 -0600
} 11, 51 -- Received: by mail-pa0-f44.google.com with SMTP id uo6so80304577pac.1
} 11, 51 -- for {microscopy-at-microscopy.com} ; Mon, 08 Feb 2016 12:38:53 -0800 (PST)
} 11, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 11, 51 -- d=gmail.com; s=20120113;
} 11, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
} 11, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
} 11, 51 -- bh=Kd5wPa3xL1KRy21HGzZ7X/1KILdCjQGptbL380mN9oo=;
} 11, 51 -- b=tthUUgpnwEQ+/+FXvI4g+6//GD35VIG65lSudPBHHCZpJF1Zpq8Qt1Mrebo6bmcn37
} 11, 51 -- xwSjCr5lu+VcwFJ0XHfYIGwSHGovcOlJ0Oh47oEVopf8p+MTg8ogCJTPSe4QI4esa+AJ
} 11, 51 -- fPVud3b56AAiM4wGwXiobGS60+ZbeP1ojP71ouqZwnsSKtpkze+NjYR10Uzt39MouYZn
} 11, 51 -- 6vHZ/Ret95BoMeDP/C6cslY18KqDGeGePG1qxJMgR4wk/VpomMzD7qL9v1zx00DTPcAL
} 11, 51 -- BVhGdB245vFuSEOq4F2HD580/i+FD9IsimEoT3f/3Q49P0fU33XMp5xvFlm1laiBWOMo
} 11, 51 -- N+Fw==
} 11, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 11, 51 -- d=1e100.net; s=20130820;
} 11, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
} 11, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
} 11, 51 -- :content-transfer-encoding;
} 11, 51 -- bh=Kd5wPa3xL1KRy21HGzZ7X/1KILdCjQGptbL380mN9oo=;
} 11, 51 -- b=ZAyLks47+lo2nsw2/whVVR8XiysNYlUQYhqwOmtEce4RVxOGomUL73+XTEs7DcaIfz
} 11, 51 -- 2uqpT+/4bVdaoQvaQAFGWdwV8u+QWxCr7yL9IYX0l8MEXljvUf5aCq9ZXtgG1j6tpErV
} 11, 51 -- T8aooEl4YPMMMsPLoeYaCw/F6OPg9oE/bgq4uGhCwiQEfxIC0xj6cWfBeanCHDZ/J9wl
} 11, 51 -- LiyKb5eziLytHxxttunij9L4tiNBYsH7xcrl2LSI1XZk7q4WrHCLAQD+PzMJid/sevla
} 11, 51 -- s/KUw82LQcefvP89VyZuP2XSsamQL01WueIezEGw+dlvoncnBTNjIrn0+I6PLuXEIdxR
} 11, 51 -- IKzg==
} 11, 51 -- X-Gm-Message-State: AG10YOTf/EYZc9TwROKoW3gLvAdNzbyO86Ti///deXL4qiIEMwIvykzVi++4beLc2F2R1A==
} 11, 51 -- X-Received: by 10.66.147.136 with SMTP id tk8mr45253166pab.157.1454963932939;
} 11, 51 -- Mon, 08 Feb 2016 12:38:52 -0800 (PST)
} 11, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([59.167.247.4])
} 11, 51 -- by smtp.googlemail.com with ESMTPSA id u5sm13127576pfi.15.2016.02.08.12.38.51
} 11, 51 -- for {microscopy-at-microscopy.com}
} 11, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
} 11, 51 -- Mon, 08 Feb 2016 12:38:52 -0800 (PST)
} 11, 51 -- Subject: viaWWW:TEM - Cryo-ultramicrotomy of rubber sections
} 11, 51 -- References: {201602081713.u18HD135013683-at-ns.microscopy.com}
} 11, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
} 11, 51 -- Reply-To: microscopy.listserver-at-gmail.com
} 11, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
} 11, 51 -- X-Forwarded-Message-Id: {201602081713.u18HD135013683-at-ns.microscopy.com}
} 11, 51 -- Message-ID: {56B8FCD9.5000703-at-gmail.com}
} 11, 51 -- Date: Tue, 9 Feb 2016 07:38:49 +1100
} 11, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
} 11, 51 -- Gecko/20100101 Thunderbird/38.5.1
} 11, 51 -- MIME-Version: 1.0
} 11, 51 -- In-Reply-To: {201602081713.u18HD135013683-at-ns.microscopy.com}
} 11, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
} 11, 51 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================



==============================Original Headers==============================
8, 38 -- From leunissen-at-aurion.nl Mon Feb 8 16:06:44 2016
8, 38 -- Received: from nm6-vm7.access.bullet.mail.bf1.yahoo.com (nm6-vm7.access.bullet.mail.bf1.yahoo.com [216.109.114.150])
8, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u18M6gFP012755
8, 38 -- for {microscopy-at-microscopy.com} ; Mon, 8 Feb 2016 16:06:43 -0600
8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1454969189; bh=5CcktKX92OvC/tfr4Zhvb2o/3hryqQPIIo0UZ5ZKDDk=; h=Subject:From:In-Reply-To:Date:Cc:References:To:From:Subject; b=IM1I0QyuVljo+ccZqg+K37rcVdQl29WJ9vmFsRgw5hVvDM0iWJ6+SWNm1TTSND+CaT8KgB76B2ZFxSL0R0bl0A2G81ROlWQyuAfVpE4oE/E3vH5i/zAlfDS73UX5E/UcmdI6QDOtG2sj9vfyINRMOgdNpwD8c0sguyFTVuvpmzjcO/pDs9rza9dr18JnKmoWp7HqBcaaxpNSfNCIGGy2rKy4A5UErCcsTO2YAPJayvaM5NQeBlWZ2N3/ulKryIVjqPBg+KNBDOh3o9uB8Rj0iuB9/rYmXsCTVPLb+m4o5DYgiEOAs6wOT6TcnEg3kl3BqlXui/vJAaMHP6/KV5E5Xw==
8, 38 -- Received: from [66.196.81.164] by nm6.access.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2016 22:06:29 -0000
8, 38 -- Received: from [124.108.96.24] by tm10.access.bullet.mail.bf1.yahoo.com with NNFMP; 08 Feb 2016 22:06:29 -0000
8, 38 -- Received: from [127.0.0.1] by omp1001.tnz.mail.aue.yahoo.com with NNFMP; 08 Feb 2016 22:06:28 -0000
8, 38 -- X-Yahoo-Newman-Id: 723320.45878.bm-at-omp1001.tnz.mail.aue.yahoo.com
8, 38 -- Received: (qmail 33870 invoked by uid 1000); 8 Feb 2016 22:06:28 -0000
8, 38 -- Received: from 124.108.96.119 by rel109.mail.aue.yahoo.com with SMTP; Mon, 08 Feb 2016 22:06:28 +0000
8, 38 -- Received: (qmail 30454 invoked from network); 8 Feb 2016 22:06:28 -0000
8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s1024; t=1454969188; bh=5CcktKX92OvC/tfr4Zhvb2o/3hryqQPIIo0UZ5ZKDDk=; h=Content-Type:Mime-Version:Subject:From:In-Reply-To:Date:Cc:Content-Transfer-Encoding:Message-Id:References:To; b=fiFcNGAtFzcvC1sdBMedTzxosX8EG6cY+3YI964f0JT2geDTyXR3GSixv1w3LGYTF3prV+k4mRW+k8C3SCQgf8eCSaUdy0UkcJX2iZh2wYI4Y3G0PnpHBLURZHVJ1b8etcENiF1d99HI2A2JJRPt/PcRgfMEz/a3qvqb9MnlZ3M=
8, 38 -- X-Yahoo-Newman-Property: ymail-3
8, 38 -- X-YMail-OSG: rq1ahn0VM1kgoVAZKyfIvXbQ_Aqvmwcu8iyyJlT_aTn1_7r
8, 38 -- 7dkY7JiG8Wtvdgs939FPO7d0XoAK1tH.kl2UK64i4axQL4zEX7U_dJgBuL1z
8, 38 -- 4ToOKqlT4iSphgNf5nPpC8idg.PklZliIANd8qPbB_Whhh.O6OAfnz8a9xbC
8, 38 -- tF007IgVq9j95lv2nBEaumiqjIGdaWeH0wBM1hTsFfpY_ko8EIyRA.jMl2nS
8, 38 -- 6mM5MUlu.IcILgop1vS7s9929Cl9jWNNJW0N1xxGvkYCMRvXpTo.6Zie1UWy
8, 38 -- T0QXRv85PUjvVfx6Dz3g89nFtzmMKFqR9BABRiBBUeZX_R6KmcYQrcIa.z0O
8, 38 -- 8UYhreHKLuRS41nY20_BguS0Du.whts1M0.iCOe89Zv0.sRqeWTRTIoVezpA
8, 38 -- eR5e5wCyW_mPuRfCyqkeNDkI1JJNhJqQ5KyMG4z7DQQcU.BHNQN_N07zbBKn
8, 38 -- W6YjLINMnp9r1RkG__CabCeDfvu1zjwDh7ss8KoO1ZqOWzskMjIOsjExv0_w
8, 38 -- b4ok6GhEXr0.hdWn6RrTfD1Jp9yTR7dKYR8Zw5LlP_1_m.ujA
8, 38 -- X-Yahoo-SMTP: E1TINkOswBCOniPaNnp23qBGQpLN4KsU.cr0KdlJlWhF
8, 38 -- Content-Type: text/plain; charset=us-ascii
8, 38 -- Mime-Version: 1.0 (Mac OS X Mail 8.2 \(2104\))
8, 38 -- Subject: Re: [Microscopy] viaWWW:TEM - Cryo-ultramicrotomy of rubber sections
8, 38 -- From: Jan Leunissen {leunissen-at-aurion.nl}
8, 38 -- In-Reply-To: {201602082041.u18KfFGe021301-at-ns.microscopy.com}
8, 38 -- Date: Tue, 9 Feb 2016 11:06:26 +1300
8, 38 -- Cc: badamianand-at-bfusa.com
8, 38 -- Message-Id: {D8629196-A927-4DF2-BD24-5C1E3F243E1F-at-aurion.nl}
8, 38 -- References: {201602082041.u18KfFGe021301-at-ns.microscopy.com}
8, 38 -- To: microscopy-at-microscopy.com
8, 38 -- X-Mailer: Apple Mail (2.2104)
8, 38 -- Content-Transfer-Encoding: 8bit
8, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u18M6gFP012755
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Tue, 9 Feb 2016 15:27:21 -0600
Subject: [Microscopy] viaWWW:ipj oxford eds file

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: nlamine6-at-hotmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both nlamine6-at-hotmail.com as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: nlamine6-at-hotmail.com
Name: NAITBOUDA ABDELYAMINE

Organization: CDTA

Title-Subject: [Filtered] ipj oxford eds file

Message: Does any body know a tool/library for opening the Oxford Inca
EDS project files (.ipj)? or to convert to open eds file

Login Host: 41.110.187.86
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
13, 51 -- From microscopy.listserver-at-gmail.com Tue Feb 9 15:27:19 2016
13, 51 -- Received: from mail-pa0-f53.google.com (mail-pa0-f53.google.com [209.85.220.53])
13, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u19LRJbm029638
13, 51 -- for {microscopy-at-microscopy.com} ; Tue, 9 Feb 2016 15:27:19 -0600
13, 51 -- Received: by mail-pa0-f53.google.com with SMTP id kp3so72594pab.1
13, 51 -- for {microscopy-at-microscopy.com} ; Tue, 09 Feb 2016 13:27:06 -0800 (PST)
13, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 51 -- d=gmail.com; s=20120113;
13, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
13, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
13, 51 -- bh=ZG7yN6CoQLZa++zV1QsH0FDMb+pov10FRbxj6qfXrxM=;
13, 51 -- b=B4I/G7cAYJV1dkFvzO1IUfzV/KK7HiA44vi/TZTMViiVJbVmKKz/HU/BFBuvp5oQ5c
13, 51 -- NXgP+lNDnSiXi9gbyydGETLQp3Vg0KNEJcTpCcdG6R75T8T6SGTETaoCUZxkFoaIWp0S
13, 51 -- y//FcGBf0sQtwuYfdrezrhT//ZKtA2kaQdS83qzs/IwSBpDaddjoBMNvwqw5WiMpTrfX
13, 51 -- YdsTNxsJ48h/fw+QyOsg5PQ4XwiQyOFDOMgbcYJ2xD2Uo4LDj/tB/v6mqbquyiqSnVWZ
13, 51 -- eFicSIiaPuNct5cbdJpvDWAw1avTYm5ETiQ0NA2O5qaMNfL1eDyR5ipeVAwXen4oNna1
13, 51 -- caHQ==
13, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 51 -- d=1e100.net; s=20130820;
13, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
13, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
13, 51 -- :content-transfer-encoding;
13, 51 -- bh=ZG7yN6CoQLZa++zV1QsH0FDMb+pov10FRbxj6qfXrxM=;
13, 51 -- b=hwkGgAAi2fztfYWfgTcA1lDSa9SH3iLgfgA2tXW28MrBnUgrFZwo9rhyzmy6HMM+aQ
13, 51 -- AmamRWPDfVjYqLDJhoLYR4oKTPUOeY58zTHQ98ICG/NXN4zN5Ik6tgv128fhrFRVEFDJ
13, 51 -- BoTlSSZaPWIcYQ/PwedjPfDbo2pk5M/E+gOoSwlaHbfVs14nMGVBdmibVvKzbNuvTy9I
13, 51 -- ziGH0G+0VR80LR3J572DLGTpMhOaoOTJtawcbRKPhWBre5Yym+SFy7j1kauwawNcwwki
13, 51 -- yqrqeRxcZxoV8Zi7SxCzjEUaZLhVC9OLzyCeQ3GyYxlpRzjVvCBrV3Q78EckMAPVG7qD
13, 51 -- 3BUg==
13, 51 -- X-Gm-Message-State: AG10YORnFhIdBwrGHLiWcJl/tBYnO+CCfP7AVTPAD7MoRFQN7YCD6S3XKoDj9i/B9w+EGg==
13, 51 -- X-Received: by 10.67.15.73 with SMTP id fm9mr54233976pad.130.1455053226495;
13, 51 -- Tue, 09 Feb 2016 13:27:06 -0800 (PST)
13, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([137.154.167.235])
13, 51 -- by smtp.googlemail.com with ESMTPSA id qj8sm52671237pac.40.2016.02.09.13.27.04
13, 51 -- for {microscopy-at-microscopy.com}
13, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 51 -- Tue, 09 Feb 2016 13:27:06 -0800 (PST)
13, 51 -- Subject: viaWWW:ipj oxford eds file
13, 51 -- References: {201602091517.u19FH2XS013183-at-ns.microscopy.com}
13, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 51 -- Reply-To: microscopy.listserver-at-gmail.com
13, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 51 -- X-Forwarded-Message-Id: {201602091517.u19FH2XS013183-at-ns.microscopy.com}
13, 51 -- Message-ID: {56BA59A6.3040100-at-gmail.com}
13, 51 -- Date: Wed, 10 Feb 2016 08:27:02 +1100
13, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 51 -- Gecko/20100101 Thunderbird/38.5.1
13, 51 -- MIME-Version: 1.0
13, 51 -- In-Reply-To: {201602091517.u19FH2XS013183-at-ns.microscopy.com}
13, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: smithj-at-winthrop.edu
Date: Wed, 10 Feb 2016 07:28:06 -0600
Subject: [Microscopy] Need contact information for Leica Microscopy rep, South Carolina,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sorry to spam your in-boxes folks.

My response to Harry.Clark-at-leica-microsystems.com has bounced twice with
"content rejected"

Need a quote on a basic upright biological microscope with
transmitted-light DIC (DM2500).

If you're the person mentioned in the subject line, please respond to my
address below.

Thanks,
Julian

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
349 Columbia Ave
Rock Hill, SC 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net


==============================Original Headers==============================
8, 78 -- From smithj-at-winthrop.edu Wed Feb 10 07:28:03 2016
8, 78 -- Received: from na01-bl2-obe.outbound.protection.outlook.com (mail-bl2on0148.outbound.protection.outlook.com [65.55.169.148])
8, 78 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1ADS0Yv022603
8, 78 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 07:28:01 -0600
8, 78 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 78 -- d=winthropu.onmicrosoft.com; s=selector1-winthrop-edu;
8, 78 -- h=From:To:Date:Subject:Message-ID:Content-Type:MIME-Version;
8, 78 -- bh=LURwZr768z5lxqA7++UgzpB9/rdnnQj1sJR4ut9dRBo=;
8, 78 -- b=AAydgtqzB12kwVOSJYWBgYZPFhezP4drlPOXG1N5395/rT0iIcEod8ZlqficZHJYxteuoeIQ2JrwsBr2ROXiGUMa4gLNYLXJuZgLIWZAXUaDDsLMr5a8UlKqLfqb3CNFRZqJmaMkceXpx+hHozbAmokp9euXEaqsfRkadIadZ08=
8, 78 -- Authentication-Results: microscopy.com; dkim=none (message not signed)
8, 78 -- header.d=none;microscopy.com; dmarc=none action=none
8, 78 -- header.from=winthrop.edu;
8, 78 -- Received: from administrators-iMac-2.local (206.74.212.47) by
8, 78 -- BY2PR10MB0742.namprd10.prod.outlook.com (10.163.110.28) with Microsoft SMTP
8, 78 -- Server (TLS) id 15.1.403.16; Wed, 10 Feb 2016 13:27:39 +0000
8, 78 -- From: Julian Smith III {smithj-at-winthrop.edu}
8, 78 -- Subject: Need contact information for Leica Microscopy rep, South Carolina,
8, 78 -- USA
8, 78 -- Message-ID: {56BB3AC6.5010002-at-winthrop.edu}
8, 78 -- Date: Wed, 10 Feb 2016 08:27:34 -0500
8, 78 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.6; rv:38.0)
8, 78 -- Gecko/20100101 Thunderbird/38.5.1
8, 78 -- MIME-Version: 1.0
8, 78 -- To: undisclosed-recipients:;
8, 78 -- Content-Type: text/plain; charset="utf-8"; format=flowed
8, 78 -- Content-Transfer-Encoding: 7bit
8, 78 -- X-Originating-IP: [206.74.212.47]
8, 78 -- X-ClientProxiedBy: BN3PR0401CA0038.namprd04.prod.outlook.com (25.162.159.176)
8, 78 -- To BY2PR10MB0742.namprd10.prod.outlook.com (25.163.110.28)
8, 78 -- X-MS-Office365-Filtering-Correlation-Id: ecb5597d-1a09-4ff4-b2d6-08d3321df2e8
8, 78 -- X-Microsoft-Exchange-Diagnostics: 1;BY2PR10MB0742;2:lHK6e2H0OsNB0Ta9MlvLF0CJgUxWqkGiYMNRMsiOMCb2NRg66VL6shdi1A1QscSzoaweuinRPBNDfBk+LSCjiLC9WPIcBC+e9IQoAaqpntzS0kCd9w1SlTBUZXtEOig8Lux4PH1ZneszYGLepH0ATZl2iPz503Rbir6zc4N49EIqatJvmzzL1bT/PTed56D5;3:FSq9yxx0/PbqT3CIjLAvtwXsby7uSTxPOcB4QAqm/EGidHug6s4dlWs5T8FKKlLHeO4kajes3g7tCPdtxD0KY2A1pcwactb0ByIKDWkGAO/uLlUtzi+SfGifqy96VLzU;25:Pj5Irb5PTLsXQv0hP/8abNyG5YVx6xnFEg4RNzVnunt4v13nohkfwOwEEIGSVCka8cDCfannQYhQl9/rzYiosFQKyQ55YbDN2I+3d1siLtE7hx+lM32Twq5ovH3Lgv+R9r5Ac2NPDMAN/ez9zzUeXEAJHK54y+MOjOsJ4XGZWGuKfrzdcEKxLrEZTTt5qgeKOTD/e5fve5Io0IWGjh/EVcLZzvNA0GrWX3BFp8sLw1NeA6XXb9LAeRR9CU2MKbEIURRSzxq8LTe5Yc0nFF5T1UbPHpAQaejHaz3h5aSoM32rgLeeqqxzBVCopffWK782
8, 78 -- X-Microsoft-Antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BY2PR10MB0742;
8, 78 -- X-Microsoft-Exchange-Diagnostics: 1;BY2PR10MB0742;20:+kP2JlGOjPmt/c3Izit7wjyJAG2edriokg0n2jYVFzFug+YXl7kBoPulSZXd6Nibg/pY6phEYRHM+Ur0iJM+OetGXbRnTpe0XQqzrUTZ0eHL15VRwkXaDnW2jYOE9to0ytNrPglCeKb7pouNHBHm5gs/uZ9V4qG4vdvEfCmwOtYDA3V4ny8HxlX9qYv0mQnWgiZMOLpNZx52aQVaXj8V9/6EMAjqOtATa/hauBPkWJyqQaIbKc+EIkXgTXEikV/SdLFH8wjZTrG1CQCg0xo3xWPhklowNFb+46qbHJOtQUUq850uMnZ4ewSpMJI18c0crS0QGlge/v4re5aA5AKmng==;4:Z5UbYJn+8NSyevATtEHf7pMe3AEurpt/oqsLb+sfoY0xNAE7fuqe2qMMGQRp6DfNP1aWcynJIagw/q7VronhZwcCQAzoUuBFInQ7Yl3JaRIbAqAxFTk/x6kryXNSuxh1F/MXn5wyYhhHv/kY5e+7mFVy6Og60L+GkXqdx4FbK2lRJfae3hY0S5whc4hbRvOeGeA0DA0lyuZkeA54nLPnDWz3P4hiNfGLgJjEjfOBnUxfFG4k/oqn3PzeMbQ0UxSchtzrtHig6HNq5xnI8mtiuvbDxbuk4YHwyqXgV4PXNPy0emaDxZQOWIrPjmM2PJ3gpGRDPNNXj1HulXtV9sMhqP2rDtQcwPNqcfv0KOdHv1l+k+lqPduEwuzLggkoxLOp
8, 78 -- X-Microsoft-Antispam-PRVS: {BY2PR10MB074291686BBD11A606576838A0D70-at-BY2PR10MB0742.namprd10.prod.outlook.com}
8, 78 -- X-Exchange-Antispam-Report-Test: UriScan:;
8, 78 -- X-Exchange-Antispam-Report-CFA-Test: BCL:0;PCL:0;RULEID:(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046);SRVR:BY2PR10MB0742;BCL:0;PCL:0;RULEID:;SRVR:BY2PR10MB0742;
8, 78 -- X-Forefront-PRVS: 0848C1A6AA
8, 78 -- X-Forefront-Antispam-Report: SFV:NSPM;SFS:(10019020)(6009001)(54504004)(164054003)(30584003)(30594003)(5004730100002)(59896002)(19580405001)(80316001)(50466002)(64126003)(50986999)(122386002)(15974865002)(23676002)(107886002)(89122001)(5008740100001)(5001960100002)(109986003)(19580395003)(47776003)(4001450100002)(65956001)(65806001)(86362001)(881003)(6116002)(87266999)(66066001)(83506001)(90282001)(42186005)(586003)(54356999)(223583001)(229853001)(65816999)(3846002)(75432002)(33656002)(92566002)(189998001)(88552002)(230700001)(4001350100001)(1096002)(87976001)(36756003)(77096005)(2906002)(110136002)(40100003)(18886075002);DIR:OUT;SFP:1102;SCL:1;SRVR:BY2PR10MB0742;H:administrators-iMac-2.local;FPR:;SPF:None;MLV:nov;PTR:InfoNoRecords;LANG:en;
8, 78 -- X-Microsoft-Exchange-Diagnostics: =?utf-8?B?MTtCWTJQUjEwTUIwNzQyOzIzOmRBTXBaSDErQ2dYQ09oSlhMdEp6dkNoYW12?=
8, 78 -- =?utf-8?B?OXQ1L3ZoVUFodHA0VFNqYmlFODZrVzVvc3RsQlBWR1RtS1I3WFROZlVtWEZ4?=
8, 78 -- =?utf-8?B?OEJSS2lOZVBOKzE3NTFLd0k3dUJZVGUrVXE1KzlBR0Y0cUdRSnFWcUpPSWVV?=
8, 78 -- =?utf-8?B?N2RtNzBIM2czMHNOQ0l6Yk9EWXNta0RVeVRIcEhFWk1uaDlhdDk5bU10R0Zt?=
8, 78 -- =?utf-8?B?d1JWenhVUnI4QVRtbXF1SDlXSmxQa0QxaC91Tk9JU3NLOEh6aUJ1M1g3TERo?=
8, 78 -- =?utf-8?B?UzNTQ0VqL3hEbXo2UE5acHczZmZHczg5ZmtrK292VldONDNyVG9KVGtGeUR1?=
8, 78 -- =?utf-8?B?UUcrS0VwU1dSNDI3eWx6TGJEVzNUcjROdmFyNmVqbk9aUmd5Zzl2TWNGUlNt?=
8, 78 -- =?utf-8?B?dFg0MUR3MCtvSG1sYzBwMHZseWpyaW8ra00wK1laSTMxMUVPNDBjMW54RmtP?=
8, 78 -- =?utf-8?B?bzd4RURTdGVVb3ZwVm5KWndONnlCeTZ6dU5GV2pVZ1dwdDNMSEU1Ynk2cndi?=
8, 78 -- =?utf-8?B?UCttaEtQQzR3VFowcWRlVmZJV1NYa3lSSGh1eThWUW9SQi9MYlprYksxUGlz?=
8, 78 -- =?utf-8?B?cXV2MEpDazFMZzRHM0JrbzRhckRUNElacVZMd0pjeFhRR29GRDJCbHI0NlA1?=
8, 78 -- =?utf-8?B?RVZDMlI5bkVReUJRcEczZEpHMmFSZ0J1a1RPNmJwNGdycDF5cXB2M2wxWDZT?=
8, 78 -- =?utf-8?B?TXpENlJhZlcvdEdNbk9UdU9kWGliUVVBREYxZW9wWmtkSGZZb1JOQlVOL2hP?=
8, 78 -- =?utf-8?B?Z2YrYjZYdWRpSE14MUtkOExuOGVRNXEyZG9TVXpUZ1VMRnVtSHNjYXpsMHJ6?=
8, 78 -- =?utf-8?B?YVV6MWJQbGxsbnVXM3gvR294b3BQS0hCL3VsakJ3MFd2VlBmeFZoRWlWOVUx?=
8, 78 -- =?utf-8?B?aXR4OVhkczJXT1BMblRpaWlJWFM1Z2lVcE03ZWVBeWdNVHpqSG01QkRwc1Bo?=
8, 78 -- =?utf-8?B?aXZRWERaNnVXM21Rc3FuMnk0SlRVNTBkLzhkSVdhT0FpWGF4aWdKTWNxeVlK?=
8, 78 -- =?utf-8?B?ajJpZEpQdTNsa09CakJjU29oOVVBSVRjVHFZeXB4U21rUDhFZm5OMkZUVTR2?=
8, 78 -- =?utf-8?B?ZWE1RStRdmNnOG5rWmN1V3lJSjZHTnYveEJCaVhjcVFvTi8xQU9yRmE5dXQ1?=
8, 78 -- =?utf-8?B?OGU5Zk9yOWZIcVhLNDBZdlI4WC9KMDIvWWFXemNONjlkUy9QZUh2VVJPY0xh?=
8, 78 -- =?utf-8?B?TlI5cFBxUWZQMytGMTk3ZFBKTzdDMWpiYTE2cDErMzVHVGE4bmFtRDkxUkFM?=
8, 78 -- =?utf-8?B?UEk2WGpSdWlkaHgxYVNuOFFZZUZTQ1ozNFYvVXZFSTFsZVUrU1ZJazQ0cTJa?=
8, 78 -- =?utf-8?B?aWJpNHE5ZGwxblRZNFp6elF6cStWUGsrd2N0Qjdjc21wZ3NvZFhSbEZ4TWl5?=
8, 78 -- =?utf-8?B?alBVRlNyRFY3Q3F2aW9ReUdHdTk1NUpic0h0OTVUTWVUWjIxQzFiM2I2N25m?=
8, 78 -- =?utf-8?B?d3VGajhzWEg5djBHb3NxaUMxYWJwanRBWm9EcWUxZUt1UEZlRlNEV2xJNFJD?=
8, 78 -- =?utf-8?B?TFJlL3ZsN3hRQk44UE92QTRnWlNGNlFoc1lyelZnMVRuNStPaGN2c3Ftcnhu?=
8, 78 -- =?utf-8?B?bXZNa2NFcFQ4UEZSUnBjVVBnbUYreXl3QkVKZ3poS3FTUGkzb3dwUjRLNEZ5?=
8, 78 -- =?utf-8?B?WS9ZME1RMis5UnlOMVpDakRubGVyTUJadDlnQlpKVmYvZDNoODZCUktpL0o5?=
8, 78 -- =?utf-8?B?anhwdU8xNEtpdEtlcW12OWl2MExDMlZHb3dxaVhPdWphSERHa3Y2Uk4zbG9W?=
8, 78 -- =?utf-8?B?T01nZDVKYW4xMGFQczRyZmI2dTRnZnlNUERUMnZwM1lkR1ZGUXBrQWtmTUZh?=
8, 78 -- =?utf-8?B?cUsvVVBnOExYYStKU3BQKzJEUW9BUFNqeTJHM1RPZ2F0SFdOb3VETU1STWNY?=
8, 78 -- =?utf-8?Q?CWH1GbDDgVzRckmwT2ih3UddRzcv2rBo?=
8, 78 -- X-Microsoft-Exchange-Diagnostics: 1;BY2PR10MB0742;5:l25tuDT0OdrWlos2oDHf7jPIDEVXnAY+Qs5FuOwKRsK+/yr82f9UnOeymwMhRX2WWWi3A8XSoReYueizCxQlwsDFkrfcvTcQG4+uWHO2kvWoFEsvaYAG+nvOD1/eC6a1OMz5+VwaBbNfwbQzC3BxFA==;24:5Gr1hY4LfjP1+X/Q6heukRMYTgeYC/UYf92WbwUIBYB07UgHnB6YPRNhM+JIa2WfmMcKNYec28zI5srCwqUcwM8PnkUn564QRDlU3Y7AvO4=;20:5ubIHVzPHWmDk+4fvXjtD5xY7yUtQRldm08hWA7jr7VV81K1NwJNVXDTe6oKXSsmn8kxxICCqVL5OdhvYqg4KHHVqYpvg+8a4aQ2D36zOIqQUPrezxpOifQJJOp2gQ5uyA8LEuu3x2BheSvr42XurGyivK1tHOAW3yKL6JPDY4c=
8, 78 -- SpamDiagnosticOutput: 1:23
8, 78 -- SpamDiagnosticMetadata: NSPM
8, 78 -- X-OriginatorOrg: winthrop.edu
8, 78 -- X-MS-Exchange-CrossTenant-OriginalArrivalTime: 10 Feb 2016 13:27:39.7179
8, 78 -- (UTC)
8, 78 -- X-MS-Exchange-CrossTenant-FromEntityHeader: Hosted
8, 78 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BY2PR10MB0742
==============================End of - Headers==============================




From: ALawrence-at-i2at.msstate.edu
Date: Wed, 10 Feb 2016 14:04:40 -0600
Subject: [Microscopy] M&M 2016 student and volunteer meeting assistance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The hotel reservation link is open and the extended deadline for paper submission is less than a week away. As you (or your students) are making plans to attend the Columbus meetings (July 24-28), please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.

The students will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve such things as providing support in the different symposia (assisting with audio-visual needs, speaker set-up, maintaining an attendance count), staffing the volunteer office, and helping with vendor tutorial sign-up. Payment is given as a check at the end of the meetings or when the student leaves Columbus.

Once the program has been finalized, each registered bursary will be contacted and allowed to choose the times and activities they would like to work. Many times they end up "working" sessions they would attend anyway. There is an added bonus of $10 cash to help with meals for each morning and/or afternoon session worked and a meeting shirt.

If anyone would like to participate in the bursary program be sure to check the "I wish to apply for a student bursary" box in section 2 of the registration form (registration opens March 1) and send me an email of your intention. Bursary space is limited, so sign-up early. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized educational institution, and have paid their registration fee.

For those 'non-students' volunteers are also needed to help with the above mentioned meeting activities. Although not paid on an hourly basis as the student bursaries, volunteers do receive a meeting shirt and the same cash allotment to help with meals. Volunteers also have the opportunity to interact more with the microscopy community as they assist with meeting tasks.

If anyone has any questions about the bursary/volunteer program, or would like to participate, please contact me.
Amanda

Amanda Lawrence
Institute for Imaging and Analytical Technologies
Mississippi State University
662-325-7998
alawrence-at-i2at.msstate.edu




==============================Original Headers==============================
10, 31 -- From ALawrence-at-i2at.msstate.edu Wed Feb 10 14:04:39 2016
10, 31 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120])
10, 31 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1AK4cDQ026751
10, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 14:04:38 -0600
10, 31 -- Received: from mail06.ad.msstate.edu (mail06.ad.msstate.edu [130.18.230.65])
10, 31 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id u1AK4OMZ008327
10, 31 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL)
10, 31 -- for {Microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 14:04:25 -0600
10, 31 -- X-Sender: {}
10, 31 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by
10, 31 -- mail06.ad.msstate.edu (2002:8212:e641::8212:e641) with Microsoft SMTP Server
10, 31 -- (TLS) id 15.0.913.22; Wed, 10 Feb 2016 14:04:21 -0600
10, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by
10, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id
10, 31 -- 15.00.0913.011; Wed, 10 Feb 2016 14:04:21 -0600
10, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu}
10, 31 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
10, 31 -- Subject: M&M 2016 student and volunteer meeting assistance
10, 31 -- Thread-Topic: M&M 2016 student and volunteer meeting assistance
10, 31 -- Thread-Index: AdFkPWfcCSeNajF7SCqWm3W6g3+d4g==
10, 31 -- Date: Wed, 10 Feb 2016 20:04:20 +0000
10, 31 -- Message-ID: {4ad3a9b7cfc94ddbb127cf560d67af83-at-mail02.ad.msstate.edu}
10, 31 -- Accept-Language: en-US
10, 31 -- Content-Language: en-US
10, 31 -- X-MS-Has-Attach:
10, 31 -- X-MS-TNEF-Correlator:
10, 31 -- x-originating-ip: [130.18.230.93]
10, 31 -- Content-Type: text/plain; charset="us-ascii"
10, 31 -- MIME-Version: 1.0
10, 31 -- Content-Transfer-Encoding: 8bit
10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1AK4cDQ026751
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:40:09 -0600
Subject: [Microscopy] viaWWW:Stanford Imaging Workshop: Advanced applications of high speed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: alkoh-at-stanford.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at
http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both alkoh-at-stanford.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: alkoh-at-stanford.edu
Name: Ai Leen Koh

Organization: Stanford University

Title-Subject: [Filtered] Stanford Imaging Workshop: Advanced
applications of high speed imaging in S/TEM

Message: Friday, March 11, 2016
8:30 am - 5:00 pm
Stanford University
Stanford Nano Shared Facilities
94305-4088 Stanford , CA

Stanford Nano Shared Facilities (SNSF) invites you to attend this unique
S/TEM workshop demonstrating the latest high speed imaging technology
and applications specifically for in-situ TEM and 4D STEM diffraction.

This comprehensive, full-day workshop will feature lectures by leading
experts, open Q&A sessions, and live microscope demonstrations all in
the state-of-the-art SNSF research complex. The microscope sessions will
be performed on an aberration (image) corrected, monochromated FEI Titan
environmental (S)TEM with a OneView in-situ camera.

This workshop is complimentary to all registered and confirmed
participants and includes breakfast, lunch and refreshments. Seating is
limited.

Online registration:
http://www.gatan.com/company/events/stanford-imaging-workshop-advanced-applications-high-speed-imaging-stem


Login Host: 171.66.208.134
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
17, 52 -- From microscopy.listserver-at-gmail.com Wed Feb 10 22:40:07 2016
17, 52 -- Received: from mail-pf0-f169.google.com (mail-pf0-f169.google.com [209.85.192.169])
17, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1B4e44E003565
17, 52 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 22:40:04 -0600
17, 52 -- Received: by mail-pf0-f169.google.com with SMTP id q63so23214311pfb.0
17, 52 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 20:39:52 -0800 (PST)
17, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 52 -- d=gmail.com; s=20120113;
17, 52 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
17, 52 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
17, 52 -- bh=ekPFIFOI2UfaUT/qxWC6TZy/vlQ9ZO6rLhI44ap9sRU=;
17, 52 -- b=ttEPea8yFW2ek06eghWVuDTs/VhGd73gryyckhBX5gDKXKjmNC7ufgyCeLBnCE1gDR
17, 52 -- D4B4pm8hxITURvXLI/RpYC2+RTACGsjDA5+nCM1/eEFsy9sykU0zpfwKy8VYlStFu2Vl
17, 52 -- E9MBPprdlqzEcmms8fD8fbHHJNNrSs/28Lkb5RhzeiKXT448JKPsLFszU3shF0ZVz20N
17, 52 -- vNljoCU+0SyS5jTy2WEY7y2y5ZyRr2S8pmPhpyi116PH9gPt/2tNtPxsbGx795+T/+D/
17, 52 -- VAxgzZoMQpQu+2vRZNHbzpX0OInaFKzPudplLIoeCf+/+6EuVHZE4YVs/yzIqMds2JEh
17, 52 -- P6fw==
17, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 52 -- d=1e100.net; s=20130820;
17, 52 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
17, 52 -- :date:user-agent:mime-version:in-reply-to:content-type
17, 52 -- :content-transfer-encoding;
17, 52 -- bh=ekPFIFOI2UfaUT/qxWC6TZy/vlQ9ZO6rLhI44ap9sRU=;
17, 52 -- b=F1NDIUr7gGAGdUN2qSup0gcNT52l/L5tD7WUUYzQ1ZWKiicaG60T6KiPV0ML7N/HV0
17, 52 -- 1cGqN7adsEj9TCW7/tW7xFf8FnJ088NwngcHlgG5m2cL3xhbuQ+25aksJz36I5ko2Bg3
17, 52 -- HEvW0FaIa2B/dbliDumSjK5Xa3l9iChUGIc5B6VlGX+ZthgaDdfB6ujsSfo4XFzHAcUP
17, 52 -- zS2dOGOltes2T15jRJ5cOadlNYbH3RG27vz/WFgvGj1oyjoAO8R8y4a4etYEByNhcKJ9
17, 52 -- Ptk/EtEiafIxSUA+yrE3uD1D5UWshfR943sGoi+KmrfZnbj1zdDxmd/NYD3iNctNAtfj
17, 52 -- 9+lQ==
17, 52 -- X-Gm-Message-State: AG10YORA5lY0u7WjOQpJeeYJLu+tQXMpDz1ADHV53cbDtaqYonsEK4ZPBcqdABOJQiutZg==
17, 52 -- X-Received: by 10.98.8.219 with SMTP id 88mr6352619pfi.51.1455165591181;
17, 52 -- Wed, 10 Feb 2016 20:39:51 -0800 (PST)
17, 52 -- Received: from Nestors-MacBookAir-Pro-2014.local ([137.154.167.196])
17, 52 -- by smtp.googlemail.com with ESMTPSA id yx4sm8581379pac.5.2016.02.10.20.39.49
17, 52 -- for {microscopy-at-microscopy.com}
17, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 52 -- Wed, 10 Feb 2016 20:39:50 -0800 (PST)
17, 52 -- Subject: viaWWW:Stanford Imaging Workshop: Advanced applications of high speed
17, 52 -- imaging in S/TEM
17, 52 -- References: {201602100001.u1A01o5E024003-at-ns.microscopy.com}
17, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 52 -- Reply-To: microscopy.listserver-at-gmail.com
17, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 52 -- X-Forwarded-Message-Id: {201602100001.u1A01o5E024003-at-ns.microscopy.com}
17, 52 -- Message-ID: {56BC1092.20502-at-gmail.com}
17, 52 -- Date: Thu, 11 Feb 2016 15:39:46 +1100
17, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 52 -- Gecko/20100101 Thunderbird/38.5.1
17, 52 -- MIME-Version: 1.0
17, 52 -- In-Reply-To: {201602100001.u1A01o5E024003-at-ns.microscopy.com}
17, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
17, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:43:01 -0600
Subject: [Microscopy] viaWWW:Microscopy Sales Opportunity- Bay Area

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: md-at-personifysearch.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both md-at-personifysearch.com as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: md-at-personifysearch.com
Name: Mary Southgate Dickson

Organization: Personify

Title-Subject: [Filtered] Microscopy Sales Opportunity- Bay Area

Message: Good Afternoon!

We have recently had a world leading microscopy client open up a few
microscopy sales opportunities in the greater Bay Area. The ideal
candidate would have an expertise in microscopy and some sales experience.

Please contact me directly to learn more at - md-at-personifysearch.com

Thanks!

Mary Southgate Dickson
Talent Management Executive
Personify
401 Harrison Oaks Boulevard
Suite 350
Cary, North Carolina 27513
www.personifysearch.com
800.875.6188 x 130
http://www.linkedin.com/in/marysouthgatedickson

Login Host: 70.63.131.242
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
15, 51 -- From microscopy.listserver-at-gmail.com Wed Feb 10 22:43:00 2016
15, 51 -- Received: from mail-pf0-f176.google.com (mail-pf0-f176.google.com [209.85.192.176])
15, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1B4gxjB003817
15, 51 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 22:42:59 -0600
15, 51 -- Received: by mail-pf0-f176.google.com with SMTP id c10so23594827pfc.2
15, 51 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 20:42:46 -0800 (PST)
15, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=gmail.com; s=20120113;
15, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
15, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 51 -- bh=i9bwy761fKo589e899Eljq54cRPHprNLxOZnV5X7w2U=;
15, 51 -- b=qUZq1jJw450j1zhqfVkvWgUX7ncP2eWqDnUt5Y4cL8nRnv/LmMOPdEvK/JSMVNqcdb
15, 51 -- GvbxV+TLycrfFb57n+8xFtgKuPnkMwQfDcOOKj6uZN/rJxVFXFqQUvDRan7iz2WhxzAM
15, 51 -- tqOkbL0PfkS+vMQNzslMWsGQDWx28/Cnuq44QEO+4BD+7TeUGc1empC7ekqdlTu4Nnx1
15, 51 -- CiqVci9tiFKtjtqzIDFCYR1/M6AFmot/ZRy+Lk+XzGtMGyEdl+Wn4jq1LgNMwbdeX/nX
15, 51 -- ItxD/265l2RDldFsmZO9wOZ3Q+Rv+wCM2MDoo4GrXJG6VrGLg/EGhnxErB0R0A3gXgCM
15, 51 -- k+9Q==
15, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=1e100.net; s=20130820;
15, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
15, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
15, 51 -- :content-transfer-encoding;
15, 51 -- bh=i9bwy761fKo589e899Eljq54cRPHprNLxOZnV5X7w2U=;
15, 51 -- b=SbyGI+Ez2qW4yQMA4kvTr+B88x5siRN089e5posLqP4XO5Kk9kC77Mst+CtHufK6BI
15, 51 -- bwpAcptI7ClPkD/scgHNoJchKN8oDUlmr7WTKKcPL9FtDbOo96tvaAPOBbEeiwGM4GsR
15, 51 -- wR/rwTV8Lbnncgm5FP3vitQosaicMH2XgDJJKmqChRp4BPCIzT6UrgyxMZDBbDlLCInD
15, 51 -- vMu0J1xVc1UbzIR/dHNq0SqwlJDCaX+D6IWXiWrpXs3Zl4nJpOZmG5CnliUQs0jbuL32
15, 51 -- WWtDw9Kw4XU063DDhbRYaeRmFlIaO6C4ykL++SMWhozrbSEGh+j8A8Vi2kv1qsSApbEq
15, 51 -- OwiQ==
15, 51 -- X-Gm-Message-State: AG10YORVnjwxaCb9lEh5DIOHFjwQM/BWxJ9IsiyyOIOx5r2uUVvi6boKWKBxdzyxdF9ZUQ==
15, 51 -- X-Received: by 10.98.18.215 with SMTP id 84mr64168192pfs.131.1455165766432;
15, 51 -- Wed, 10 Feb 2016 20:42:46 -0800 (PST)
15, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([137.154.167.196])
15, 51 -- by smtp.googlemail.com with ESMTPSA id l62sm8604764pfj.7.2016.02.10.20.42.45
15, 51 -- for {microscopy-at-microscopy.com}
15, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 51 -- Wed, 10 Feb 2016 20:42:46 -0800 (PST)
15, 51 -- Subject: viaWWW:Microscopy Sales Opportunity- Bay Area
15, 51 -- References: {201602101928.u1AJSCs5024243-at-ns.microscopy.com}
15, 51 -- Reply-To: microscopy.listserver-at-gmail.com
15, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 51 -- X-Forwarded-Message-Id: {201602101928.u1AJSCs5024243-at-ns.microscopy.com}
15, 51 -- Message-ID: {56BC1142.60004-at-gmail.com}
15, 51 -- Date: Thu, 11 Feb 2016 15:42:42 +1100
15, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
15, 51 -- Gecko/20100101 Thunderbird/38.5.1
15, 51 -- MIME-Version: 1.0
15, 51 -- In-Reply-To: {201602101928.u1AJSCs5024243-at-ns.microscopy.com}
15, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
15, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:42:22 -0600
Subject: [Microscopy] viaWWW:Leitz Wetzlar Orthoplan largefield microscope to give away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Linda.Davis-at-Vesuvius.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both Linda.Davis-at-Vesuvius.com as well as the Microscopy
Listserver
---------------------------------------------------------------------------

Email: Linda.Davis-at-Vesuvius.com
Name: Linda L Davis

Organization: Vesuvius Refractories R&D USA

Title-Subject: [Filtered] Leitz Wetzlar Orthoplan largefield microscope
to give away

Message: Greetings!!

We have an old but excellent research microscope that has been used
almost daily here since 1969, by the same microscopist who just retired
after 49 years. It is a fantastic scope with a very large footprint.
It is a Leitz Wetzlar Orthoplan, large field microscope. It has been
serviced by professional companies almost yearly since purchased new in
1969.

We are replacing it, but I hate to just throw this away. The oculars,
objectives, accessories may well be something others can use, if not the
entire microscope. It is a museum piece. Again, it has not been
sitting idle. Please let me know if you want the entire microscope
(VERY heavy for shipping, and likely fragile) or any of the parts. It-is
located at our R&D site, halfway between Toledo and Cleveland.


Login Host: 199.168.151.35
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
16, 51 -- From microscopy.listserver-at-gmail.com Wed Feb 10 22:42:19 2016
16, 51 -- Received: from mail-pa0-f43.google.com (mail-pa0-f43.google.com [209.85.220.43])
16, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1B4fGNc003703
16, 51 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 22:42:13 -0600
16, 51 -- Received: by mail-pa0-f43.google.com with SMTP id ho8so22885377pac.2
16, 51 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 20:41:03 -0800 (PST)
16, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 51 -- d=gmail.com; s=20120113;
16, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
16, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
16, 51 -- bh=6Z6uf86znRm8vcR78JCh9PBn1AGbKOiDNlLDJw+A9x0=;
16, 51 -- b=MkNrMSOO/fS4e6DkKPeBdLgXcIah7XW62X0eWrwQPK/KcPf5PhCN0UTINgszDweE8M
16, 51 -- EJFB4OqV1If5tg8VyaXVlQcPge8guJSMJ4gCzQs2o/RgbmW3Y8Ttioolk+D6ZAt+c7gw
16, 51 -- /qg+QMPlMyrMBBuTAbcml3KsdXjrewzflFycPgjhqTdRmo/w5ef3lU09ygCoTxoL8dZH
16, 51 -- dOnJfy+PYznx6x/s5wnEKibdU+jPsNVonGZUCHOkrf8nSSkxu/qdgdcC4QA2BpqvdXy5
16, 51 -- 6dd2jq4DNLHizHbW6PpqZiCfvvfJoEQ1rbIWmD2tC5fp1Er17AaDMvL/C+3zGhl9TbO2
16, 51 -- SRrQ==
16, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 51 -- d=1e100.net; s=20130820;
16, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
16, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
16, 51 -- :content-transfer-encoding;
16, 51 -- bh=6Z6uf86znRm8vcR78JCh9PBn1AGbKOiDNlLDJw+A9x0=;
16, 51 -- b=BbqDV4fJRY8OgtzYZACyaClnhOw5sh6VUvV1r7nUuLj1VQWtAE93N8Yiko+BieycVs
16, 51 -- H+3od5NlhtaouFQLK/TtXqhufPYz77X0x4PtxoYoT8IJzGtq6jxRzC98yQ2YxnoUWtRL
16, 51 -- ZD2akn0qo5A5iiFZg2Fu+pLwaOT1lO+0nPCfGOO3R6LvjTyvc8Lwf0uR2J24lQjUCzhM
16, 51 -- qmOiCHks70/eaEz4q0v91TtkG87T3UdleDCo0h6yhs4Mx0wft8uphls2kEQ3V+wy3XHw
16, 51 -- /kWBlPbdHj6EtOqlYk9+sLRkP7Zd2cvspw0kEkTtGHqTZ+r749CKUv9n5AYNCRFt6TGT
16, 51 -- PD6A==
16, 51 -- X-Gm-Message-State: AG10YOTYi3rkgVS6JznNnH0V6VoutS6vu9+G3JGTNqG028sHWvDUW/MriTOeJuMJzviFsA==
16, 51 -- X-Received: by 10.67.30.201 with SMTP id kg9mr64150731pad.132.1455165663039;
16, 51 -- Wed, 10 Feb 2016 20:41:03 -0800 (PST)
16, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([137.154.167.196])
16, 51 -- by smtp.googlemail.com with ESMTPSA id g25sm8574542pfg.35.2016.02.10.20.41.01
16, 51 -- for {microscopy-at-microscopy.com}
16, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 51 -- Wed, 10 Feb 2016 20:41:02 -0800 (PST)
16, 51 -- Subject: viaWWW:Leitz Wetzlar Orthoplan largefield microscope to give away
16, 51 -- References: {201602101341.u1ADfRfx024056-at-ns.microscopy.com}
16, 51 -- Reply-To: microscopy.listserver-at-gmail.com
16, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 51 -- X-Forwarded-Message-Id: {201602101341.u1ADfRfx024056-at-ns.microscopy.com}
16, 51 -- Message-ID: {56BC10DB.5010301-at-gmail.com}
16, 51 -- Date: Thu, 11 Feb 2016 15:40:59 +1100
16, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
16, 51 -- Gecko/20100101 Thunderbird/38.5.1
16, 51 -- MIME-Version: 1.0
16, 51 -- In-Reply-To: {201602101341.u1ADfRfx024056-at-ns.microscopy.com}
16, 51 -- Content-Type: text/plain; charset=UTF-8; format=flowed
16, 51 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Feb 2016 22:49:16 -0600
Subject: [Microscopy] viaWWW:ESEM live image to external computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: wambrose-at-unc.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both wambrose-at-unc.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: wambrose-at-unc.edu
Name: Wallace Ambrose

Organization: UNC

Title-Subject: [Filtered] ESEM live image to external computer

Message: Hello Listers
There is a video out BNC on the back of the FEI Quanta 200
ESEM. In Low Vac mode I can see the signal when connecting to an
external view monitor. The signal is horizontal lines that change in
size and number when I change image resolution or scan speed. Also B/C
seems to adjust normally. What can I do to make this into a usable image
signal to use for internet teaching outreach project? Is there a better
way to get the SEM signal to the internet?

Login Host: 152.19.204.249
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
11, 51 -- From microscopy.listserver-at-gmail.com Wed Feb 10 22:49:13 2016
11, 51 -- Received: from mail-pa0-f47.google.com (mail-pa0-f47.google.com [209.85.220.47])
11, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1B4nBMT005544
11, 51 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 22:49:12 -0600
11, 51 -- Received: by mail-pa0-f47.google.com with SMTP id dk10so1057834pac.1
11, 51 -- for {microscopy-at-microscopy.com} ; Wed, 10 Feb 2016 20:48:58 -0800 (PST)
11, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 51 -- d=gmail.com; s=20120113;
11, 51 -- h=subject:references:reply-to:to:from:message-id:date:user-agent
11, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
11, 51 -- bh=YaW0HH1ykYn6+9S/TYUiggg1Pwq3QmnqX/YpPjTIMis=;
11, 51 -- b=R0xX9IwGVT5vusWnvS7xFR0noZP0NM2vvqgx3wILZK6APQ8hyzQDUFrGG4R9i3X0VN
11, 51 -- aeWKS1f4rxSTTq+q+VXLdTzGm3uU81hlvNKcFjDsWiPYc0rHuBo3jyK+DEr/PFmS2puy
11, 51 -- l7IiKmxR/xv7WpaivVOdS4BbQfRq5fDzIuDZ66QNU/jatoOM12yGSNgW8fheKJG2fV8W
11, 51 -- JODIBYfpK8tGoqT0qJpbIdCdTPMW8uK+rZiF3W09AReSJpRoE4xXV8ADdfASj5lDAieK
11, 51 -- oy/rH/p8h4NmGaCBGZ4R8w6HnfopiT/Sk6GNgNk0bQjiPzK7crdXk8MKnAPh2keKvbkm
11, 51 -- 1urA==
11, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 51 -- d=1e100.net; s=20130820;
11, 51 -- h=x-gm-message-state:subject:references:reply-to:to:from:message-id
11, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
11, 51 -- :content-transfer-encoding;
11, 51 -- bh=YaW0HH1ykYn6+9S/TYUiggg1Pwq3QmnqX/YpPjTIMis=;
11, 51 -- b=DLvQjk4o6WlX6i5NlJUU94r4BQgkq60hjZJ+s7HQ3UgnVVQGKaRFfalDlC41Qv605z
11, 51 -- kBGONfuDpFR2ZWC2jgIQHvc9yuBYNCAGEudcVzQtoPCGGVFOeeAmFO5oXa0gVuoPhRAR
11, 51 -- EjJiV2pTWMPZeDKog7WVr+vwDpjUuqRl/Rojf2uvFZ358ysjclk/VjBj5D4/lU8OiTt7
11, 51 -- xYHna3bZZzG8ba1HQcGfqaOZEmDG5BDpaCZuU1Vy+LqRlbZ4Skzidkx7hO+/9y60AnpF
11, 51 -- CNT63Qy2ZCIk7/SbqDq3r59DDIaArMqQjdEtLDK/QAl7xYapz2IyDTWTuJa7FN9bv//k
11, 51 -- Toqw==
11, 51 -- X-Gm-Message-State: AG10YOTxied3U3apHaCGSCeKrTsRexB/nP9pSY+Ua1432Kjgs6G2ZculYKT09Ep8xqsWIQ==
11, 51 -- X-Received: by 10.66.232.132 with SMTP id to4mr63694480pac.95.1455165711169;
11, 51 -- Wed, 10 Feb 2016 20:41:51 -0800 (PST)
11, 51 -- Received: from Nestors-MacBookAir-Pro-2014.local ([137.154.167.196])
11, 51 -- by smtp.googlemail.com with ESMTPSA id 87sm2180786pfq.93.2016.02.10.20.41.49
11, 51 -- for {microscopy-at-microscopy.com}
11, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
11, 51 -- Wed, 10 Feb 2016 20:41:50 -0800 (PST)
11, 51 -- Subject: viaWWW:ESEM live image to external computer
11, 51 -- References: {201602101344.u1ADid2x024710-at-ns.microscopy.com}
11, 51 -- Reply-To: microscopy.listserver-at-gmail.com
11, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
11, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
11, 51 -- X-Forwarded-Message-Id: {201602101344.u1ADid2x024710-at-ns.microscopy.com}
11, 51 -- Message-ID: {56BC110C.7000106-at-gmail.com}
11, 51 -- Date: Thu, 11 Feb 2016 15:41:48 +1100
11, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
11, 51 -- Gecko/20100101 Thunderbird/38.5.1
11, 51 -- MIME-Version: 1.0
11, 51 -- In-Reply-To: {201602101344.u1ADid2x024710-at-ns.microscopy.com}
11, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Thu, 11 Feb 2016 08:23:25 -0600
Subject: [Microscopy] Re: viaWWW:ESEM live image to external computer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Wallace,

You can use Team Viewer or VNC to directly share desktop of your SEM
with remote computer(s).

If you want to grab video from BNC then decent video grabber card and
some coding/configuring may be needed. I've used Epiphan PCIe in the
past, though not with the FEI E-SEM that you have:

http://www.epiphan.com/products/dvi2pcie-duo/


Valery Ray - also with AIM Lab, UMDCP
=======================================
PBS&T, MEO Engineering Co., Inc.
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-296-5063
E-Mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com
UMD E-Mail: vray-at-umd.edu

On 2/11/2016 12:05 AM, microscopy.listserver-at-gmail.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} X-from: wambrose-at-unc.edu
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when
} replying
} please copy both wambrose-at-unc.edu as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: wambrose-at-unc.edu
} Name: Wallace Ambrose
}
} Organization: UNC
}
} Title-Subject: [Filtered] ESEM live image to external computer
}
} Message: Hello Listers
} There is a video out BNC on the back of the FEI Quanta 200
} ESEM. In Low Vac mode I can see the signal when connecting to an
} external view monitor. The signal is horizontal lines that change in
} size and number when I change image resolution or scan speed. Also B/C
} seems to adjust normally. What can I do to make this into a usable image
} signal to use for internet teaching outreach project? Is there a better
} way to get the SEM signal to the internet?
}
} Login Host: 152.19.204.249
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}

==============================Original Headers==============================
7, 33 -- From vray-at-partbeamsystech.com Thu Feb 11 08:23:24 2016
7, 33 -- Received: from nm31-vm2.bullet.mail.bf1.yahoo.com (nm31-vm2.bullet.mail.bf1.yahoo.com [72.30.239.10])
7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BENO4K008372
7, 33 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 08:23:24 -0600
7, 33 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455200591; bh=a0hht1gkvP2BXihI3+fAlT3Serg9gsbcb8sb6jnlRW0=; h=Subject:To:References:From:Date:In-Reply-To:From:Subject; b=spIVg3Pz6s23ESkgkU/CfkhYIzhTxyuyuhmFDdnzO3/cwsPXIQlQwJ7TX0Jnm7cP76hC0+jwu1VEA6suTi1NyNLy1WrSX/phTmdeCjrfMKXjId+qfZ8YnGRDH7NRElBH0O4Zd1glRPTLYKrYF48Gz5HoDNTpk5wq5FOrckQ7EuDgBFjsBirUXgJtVN5iGcktapp9xHbXLHWT/ELLuErxM7tFLSyoGhlkz2VyUJ/4mLFSc4XHX952t8GaAkC8X8UaQK/60miVprKBaXVG8LgmSzKfJrfwaxsw84p+s7raasBjL/EpNMdX1VNkSMaROvNHWm1cBMOy/CWUZ5jm9DabPg==
7, 33 -- Received: from [98.139.170.182] by nm31.bullet.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:11 -0000
7, 33 -- Received: from [98.139.211.204] by tm25.bullet.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:11 -0000
7, 33 -- Received: from [127.0.0.1] by smtp213.mail.bf1.yahoo.com with NNFMP; 11 Feb 2016 14:23:10 -0000
7, 33 -- X-Yahoo-Newman-Id: 991022.87580.bm-at-smtp213.mail.bf1.yahoo.com
7, 33 -- X-Yahoo-Newman-Property: ymail-3
7, 33 -- X-YMail-OSG: 9E0eSKoVM1miSumfmEVXNofqt9k2.K51w3WRG_lRjyz3Gym
7, 33 -- R.89fGhBDXHaM22vAiSZHei53AggDUWcKAII8wZG19d3nrPfchxTPXGKhvlm
7, 33 -- OWvLVYSCNW20IZKi9SiQ17q1KMG33niWPqG7xzHoN_t5TNqjVc8ktWnURZ.F
7, 33 -- vYRs9pXJMjOnivgJNtcTwqndKqkxM7Ox2t.YzoOfipcUHMHe66H0Ke8vK4Nw
7, 33 -- VLSaxNAbamrMfHHBqSPICtvyMFU5h3lnpzPz35xGcAIjnC8cuG96pQwy_AP9
7, 33 -- iQcYKqnPKc543BhQ1HP63ZCkeoEKjzPHHkO7_ZVDIz.oPt._GU9JOsHX6w7q
7, 33 -- 5h0sszRs1L3tk1J.UArBU7vblwyrqyi5VB5FBRVIFBdAdk08xPxqxgVfm82s
7, 33 -- Egos3j5jZv7JsulTiTZz8bTlQC8Nd.G1PYiwltdOGjpDBwakw9ms7aeb4_Cf
7, 33 -- 4PltEe0pKxRUZekbEa3btwfvlgjPP7D4wnGKZrEa2naMfqOg.wgE5EUyxR_w
7, 33 -- V0E0udJnYM5NN_WPeEI7hAIv21LKGFcl_tKTgbwdooZmyyu6X7r0DYQ--
7, 33 -- X-Yahoo-SMTP: uAyKK5KswBAjZhZMlPsYQD5LzI3g76eLm7jfTA--
7, 33 -- Subject: Re: [Microscopy] viaWWW:ESEM live image to external computer
7, 33 -- To: microscopy-at-microscopy.com, wambrose-at-unc.edu
7, 33 -- References: {201602110505.u1B55X86010356-at-ns.microscopy.com}
7, 33 -- From: Valery Ray {vray-at-partbeamsystech.com}
7, 33 -- Message-ID: {56BC9950.8020005-at-partbeamsystech.com}
7, 33 -- Date: Thu, 11 Feb 2016 09:23:12 -0500
7, 33 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101
7, 33 -- Thunderbird/38.5.1
7, 33 -- MIME-Version: 1.0
7, 33 -- In-Reply-To: {201602110505.u1B55X86010356-at-ns.microscopy.com}
7, 33 -- Content-Type: text/plain; charset=windows-1252; format=flowed
7, 33 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: les-at-zsgenetics.com
Date: Thu, 11 Feb 2016 10:48:47 -0600
Subject: [Microscopy] graphene TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I am looking for some advice regarding making graphene TEM grids. I've had
some success using a resist-free transfer of graphene from copper foil onto
a grid. It is the very last step that is most vexing: lifting the graphene
covered grid out of the etching solution. The grid is face-down in the
solution, floating on a graphene "raft". Picking it up proves problematic.
The filter paper pick-up method does not work because the surface tension of
the etching solution is such that the grid "runs away" from the paper. I did
grab the grids with tweezers after several tries (same issue), but by then
damage had been done to the monolayer. One paper suggested draining the
etchant away, but then the I am concerned the grid will be face-down on the
bottom of the petri dish.
Any helpful ideas?

Regards,
Larry Scipioni


Regards,
Larry




==============================Original Headers==============================
7, 49 -- From les-at-zsgenetics.com Thu Feb 11 10:48:45 2016
7, 49 -- Received: from gateway24.websitewelcome.com (gateway24.websitewelcome.com [192.185.51.162])
7, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BGmjws000462
7, 49 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 10:48:45 -0600
7, 49 -- Received: from cm2.websitewelcome.com (cm2.websitewelcome.com [192.185.178.13])
7, 49 -- by gateway24.websitewelcome.com (Postfix) with ESMTP id 267E0C0CB16BE
7, 49 -- for {microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 10:48:32 -0600 (CST)
7, 49 -- Received: from gator2013.hostgator.com ([50.87.144.13])
7, 49 -- by cm2.websitewelcome.com with
7, 49 -- id H4oW1s00N0HZY1u014oXb3; Thu, 11 Feb 2016 10:48:32 -0600
7, 49 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed;
7, 49 -- d=zsgenetics.com; s=default; h=Content-Transfer-Encoding:Content-Type:
7, 49 -- MIME-Version:Message-ID:Date:Subject:To:From;
7, 49 -- bh=EB0CfrX1XTC7UT3zDSP7gRDPIuRoS/GS8xium3+Msu0=; b=tI4TWfTrlxGC1CT/40b+Pjp9WG
7, 49 -- qKZdKwRgfU1pt4xgX6L+6vvCVV5ZBtEWd9ETsJvzNAuIkIigWm/o1GJ8LsMrz2l5f77I6yU2udKEn
7, 49 -- 0+9BSldYfuKVDVRxVNlvu4kGUXwttp79HrY9P0gks1/AC491xQnlVki7nu014r6JH84w=;
7, 49 -- Received: from zsgenetics03.z.subnet.rcn.com ([146.115.5.114]:50256 helo=LarryPC)
7, 49 -- by gator2013.hostgator.com with esmtpsa (TLSv1.2:DHE-RSA-AES256-GCM-SHA384:256)
7, 49 -- (Exim 4.86)
7, 49 -- (envelope-from {les-at-zsgenetics.com} )
7, 49 -- id 1aTuPt-000Deu-OP
7, 49 -- for microscopy-at-microscopy.com; Thu, 11 Feb 2016 10:48:29 -0600
7, 49 -- From: {les-at-zsgenetics.com}
7, 49 -- To: {microscopy-at-microscopy.com}
7, 49 -- Subject: graphene TEM grids
7, 49 -- Date: Thu, 11 Feb 2016 11:48:28 -0500
7, 49 -- Message-ID: {008a01d164ec$08d521a0$1a7f64e0$-at-zsgenetics.com}
7, 49 -- MIME-Version: 1.0
7, 49 -- Content-Type: text/plain;
7, 49 -- charset="us-ascii"
7, 49 -- Content-Transfer-Encoding: 7bit
7, 49 -- X-Mailer: Microsoft Outlook 16.0
7, 49 -- Thread-Index: AdFk7AaR4/9UxD7LRIiWEneUlfWzlQ==
7, 49 -- Content-Language: en-us
7, 49 -- X-AntiAbuse: This header was added to track abuse, please include it with any abuse report
7, 49 -- X-AntiAbuse: Primary Hostname - gator2013.hostgator.com
7, 49 -- X-AntiAbuse: Original Domain - microscopy.com
7, 49 -- X-AntiAbuse: Originator/Caller UID/GID - [47 12] / [47 12]
7, 49 -- X-AntiAbuse: Sender Address Domain - zsgenetics.com
7, 49 -- X-BWhitelist: no
7, 49 -- X-Source-IP: 146.115.5.114
7, 49 -- X-Exim-ID: 1aTuPt-000Deu-OP
7, 49 -- X-Source:
7, 49 -- X-Source-Args:
7, 49 -- X-Source-Dir:
7, 49 -- X-Source-Sender: zsgenetics03.z.subnet.rcn.com (LarryPC) [146.115.5.114]:50256
7, 49 -- X-Source-Auth: les-at-zsgenetics.com
7, 49 -- X-Email-Count: 22
7, 49 -- X-Source-Cap: enNnYWRtMDE7enNnYWRtMDE7Z2F0b3IyMDEzLmhvc3RnYXRvci5jb20=
==============================End of - Headers==============================




From: conversion.seo-at-host2015.lw-china-cdn.com
Date: 13 Feb 2016 03:53:37 +0200
Subject: re: Boost Sales with Social Media Marketing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The BNC is definitely the video signal coming out of the Quanta. The question for you is how to get the corresponding X and Y coordinate for the line. You should be able to calculate the line time from the number of pixels and dwell time. You know the number of lines from the pixel count. However, I don't think you will be able to determine where the image starts to get it all in sync.

The D connector next to the BNC is for external control as by an EDS system. I don't know the pin-outs offhand but somebody probably knows them. If you don't have such a system, I don't think I would pursue that route.

I would follow Valery's suggestion of using VNC (or Team Viewer) to monitor the existing screen. You will have to deal with port forwarding since FEI tucks their microscope behind their support computer as a firewall. But they basically do what you need as part of their RAPID support system.

Also, remember that F5 will switch between quadrant and full screen mode. Ctrl-F5 will bring the active quadrant up in full screen on the secondary monitor. I recall there is a way to specify to VNC that that screen should be shared with the rest of the world. It may be that the secondary monitor is what you want to share over the net.

Having said all that, be careful. There is a reason that FEI hid the microscope computer behind a firewall. Make sure your VNC viewers are trustworthy and that they are locked down in view-only mode.

Warren Straszheim

-----Original Message-----

Hi Wallace,

You can use Team Viewer or VNC to directly share desktop of your SEM with remote computer(s).

If you want to grab video from BNC then decent video grabber card and some coding/configuring may be needed. I've used Epiphan PCIe in the past, though not with the FEI E-SEM that you have:

http://www.epiphan.com/products/dvi2pcie-duo/

Valery Ray - also with AIM Lab, UMDCP
} X-from: wambrose-at-unc.edu
}
} This Question/Comment was submitted to the Microscopy Listserver using
} the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ----------------------------------------------------------------------
} ----- Remember this posting is most likely not from a Subscriber, so
} when replying
} please copy both wambrose-at-unc.edu as well as the Microscopy Listserver
} ----------------------------------------------------------------------
} -----
}
} Email: wambrose-at-unc.edu
} Name: Wallace Ambrose
}
} Organization: UNC
}
} Title-Subject: [Filtered] ESEM live image to external computer
}
} Message: Hello Listers
} There is a video out BNC on the back of the FEI Quanta 200 ESEM. In
} Low Vac mode I can see the signal when connecting to an external view
} monitor. The signal is horizontal lines that change in size and number
} when I change image resolution or scan speed. Also B/C seems to adjust
} normally. What can I do to make this into a usable image signal to use
} for internet teaching outreach project? Is there a better way to get
} the SEM signal to the internet?
}
} Login Host: 152.19.204.249
} Listserver Email Form V - 20120416
} ----------------------------------------------------------------------


==============================Original Headers==============================
13, 47 -- From wesaia-at-iastate.edu Thu Feb 11 14:49:14 2016
13, 47 -- Received: from na01-bn1-obe.outbound.protection.outlook.com (mail-bn1bn0100.outbound.protection.outlook.com [157.56.110.100])
13, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1BKn9Jg030834
13, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 11 Feb 2016 14:49:11 -0600
13, 47 -- Received: from CY1PR04MB1813.namprd04.prod.outlook.com (10.161.169.20) by
13, 47 -- CY1PR04MB1815.namprd04.prod.outlook.com (10.161.169.22) with Microsoft SMTP
13, 47 -- Server (TLS) id 15.1.409.15; Thu, 11 Feb 2016 20:48:56 +0000
13, 47 -- Received: from CY1PR04MB1813.namprd04.prod.outlook.com ([10.161.169.20]) by
13, 47 -- CY1PR04MB1813.namprd04.prod.outlook.com ([10.161.169.20]) with mapi id
13, 47 -- 15.01.0403.017; Thu, 11 Feb 2016 20:48:56 +0000
13, 47 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu}
13, 47 -- To: "wambrose-at-unc.edu" {wambrose-at-unc.edu}
13, 47 -- CC: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
13, 47 -- Subject: RE: [Microscopy] Re: viaWWW:ESEM live image to external computer
13, 47 -- Thread-Topic: [Microscopy] Re: viaWWW:ESEM live image to external computer
13, 47 -- Thread-Index: AQHRZNi9Mon6qy7xSke+6RvLZdVPzp8nTjwA
13, 47 -- Date: Thu, 11 Feb 2016 20:48:55 +0000
13, 47 -- Message-ID: {CY1PR04MB181366225301CC943DF8951DD7A80-at-CY1PR04MB1813.namprd04.prod.outlook.com}
13, 47 -- References: {201602111430.u1BEUErD009581-at-ns.microscopy.com}
13, 47 -- In-Reply-To: {201602111430.u1BEUErD009581-at-ns.microscopy.com}
13, 47 -- Accept-Language: en-US
13, 47 -- Content-Language: en-US
13, 47 -- X-MS-Has-Attach:
13, 47 -- X-MS-TNEF-Correlator:
13, 47 -- authentication-results: unc.edu; dkim=none (message not signed)
13, 47 -- header.d=none;unc.edu; dmarc=none action=none header.from=iastate.edu;
13, 47 -- x-originating-ip: [129.186.7.32]
13, 47 -- x-microsoft-exchange-diagnostics: 1;CY1PR04MB1815;5:fxpNz+WOZOeCKE3slr1Q2W4uIGLnLXvEIzfML56KEZHQGQRLdP6GoG1A2F+uq4dtkhbiopFq2h1sn3oRPsyHIyz5SQhB3AsCTkCmtTEd8AaMI/Xhwj1s5UIt9oMou3KK0gTind2XxGEeq29CRfZ2RQ==;24:huG+Os3ujTYcNMkrEnooY/Bb6cuUB76vOeyeK6VhjPefRCpvkTSSMikAVdktzGlyKBGbJmPR7fHqLL4wI5rzvWW781WU/2i0jy0wvyTOjFc=
13, 47 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:CY1PR04MB1815;
13, 47 -- x-ms-office365-filtering-correlation-id: c0efecda-8813-4176-21f6-08d33324c245
13, 47 -- x-microsoft-antispam-prvs: {CY1PR04MB1815FC96E557FFC0FB3394EDD7A80-at-CY1PR04MB1815.namprd04.prod.outlook.com}
13, 47 -- x-exchange-antispam-report-test: UriScan:;
13, 47 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046);SRVR:CY1PR04MB1815;BCL:0;PCL:0;RULEID:;SRVR:CY1PR04MB1815;
13, 47 -- x-forefront-prvs: 08497C3D99
13, 47 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(13464003)(252514010)(122556002)(2900100001)(586003)(2906002)(3846002)(11100500001)(189998001)(4326007)(102836003)(6116002)(88552002)(2950100001)(5008740100001)(86362001)(5002640100001)(2171001)(5001960100002)(110136002)(1220700001)(40100003)(87936001)(74316001)(1096002)(2351001)(77096005)(75432002)(19580405001)(89122001)(90282001)(76576001)(5004730100002)(1730700002)(66066001)(10400500002)(15975445007)(106116001)(3280700002)(33656002)(54356999)(76176999)(50986999)(99286002)(92566002)(19580395003)(3660700001)(2501003);DIR:OUT;SFP:1101;SCL:1;SRVR:CY1PR04MB1815;H:CY1PR04MB1813.namprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en;
13, 47 -- spamdiagnosticoutput: 1:23
13, 47 -- spamdiagnosticmetadata: NSPM
13, 47 -- Content-Type: text/plain; charset="us-ascii"
13, 47 -- MIME-Version: 1.0
13, 47 -- X-OriginatorOrg: iastate.edu
13, 47 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 11 Feb 2016 20:48:55.9271
13, 47 -- (UTC)
13, 47 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted
13, 47 -- X-MS-Exchange-CrossTenant-id: 0347d89a-0174-4dd3-adeb-3339c89c35f5
13, 47 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: CY1PR04MB1815
13, 47 -- Content-Transfer-Encoding: 8bit
13, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1BKn9Jg030834
==============================End of - Headers==============================

From donnedua0-at-gmail.com Thu Feb 11 18:10:19 2016
Return-Path: {donnedua0-at-gmail.com}
Received: from gmail.com ([210.126.22.123])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1C0AFJ2027034
for {microscopylistserverarchive5-at-microscopy.com} ; Thu, 11 Feb 2016 18:10:17 -0600
Message-ID: {64499D9D.F71C185F-at-gmail.com}



X-from: kaszas.1-at-osu.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both kaszas.1-at-osu.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: kaszas.1-at-osu.edu
Name: Andrea Kaszas

Organization: Ohio State University-OARDC

Title-Subject: [Filtered] Protocol for embedding rubber in plastic resin

Message: Anybody has a protocol for embedding rubber samples in plastic
resin? Not for cryosectioning.
How to prepare the rubber for it, for example hardening the rubber somehow?
Any suggestion would be greatly appreciated.

Andrea Kaszas
Microscopy technician
OSU-OARDC


Login Host: 164.107.85.22
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
15, 51 -- From microscopy.listserver-at-gmail.com Fri Feb 12 16:44:22 2016
15, 51 -- Received: from mail-pa0-f41.google.com (mail-pa0-f41.google.com [209.85.220.41])
15, 51 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1CMiKRP009740
15, 51 -- for {microscopy-at-microscopy.com} ; Fri, 12 Feb 2016 16:44:20 -0600
15, 51 -- Received: by mail-pa0-f41.google.com with SMTP id ho8so53558556pac.2
15, 51 -- for {microscopy-at-microscopy.com} ; Fri, 12 Feb 2016 14:44:07 -0800 (PST)
15, 51 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=gmail.com; s=20120113;
15, 51 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
15, 51 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 51 -- bh=aTqc+UryhRNmbdJiXYN7tkpkyNoA89x24kH6HHMC/QU=;
15, 51 -- b=dkP1kAEd0hb/xhIFVXoQtVfrsobelm8K6t9BcTBUWGMYaEjtSUJty/zgwFrScxf6uU
15, 51 -- 9TDwZtl7bHlUppaEZZwnw9Y0A1kODwfiPrl2KyXZpRij3nKktRLqjGgeDDZqzaB1Avzu
15, 51 -- mZVUMhb6WDh5UD8cEG7chuFv5e8vfg74CM+lyy/lRDJQBUbazRBFArazIu2sfmhfbTGR
15, 51 -- eSDSkTbtxLqpstEuS66WAbGhQs+hQ4ac0s5kUETRro5lQHpJc+Yaa3OfHoQNevJTS+iV
15, 51 -- LcUnySG+1H9hKbkSHliyfA4AdmCfFXkCK5GAIFen0yE73yVGxTmWJVtsFNlDT1DPQ70D
15, 51 -- Va9w==
15, 51 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 51 -- d=1e100.net; s=20130820;
15, 51 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
15, 51 -- :date:user-agent:mime-version:in-reply-to:content-type
15, 51 -- :content-transfer-encoding;
15, 51 -- bh=aTqc+UryhRNmbdJiXYN7tkpkyNoA89x24kH6HHMC/QU=;
15, 51 -- b=Nm868/iaO/XhEO6zwdzW9XCoTj3URV8dq93P02fVzp3kv8VREd6+cJvc71SXIlYcLh
15, 51 -- 17mchwadTF8DkZWd7/A8CMQ/soNV2H8k67toS7UVutckQE4GcEKL+pXst2aXQ+up4kps
15, 51 -- 6WxvYwCmRJnu4V7+aUixlE8j4M+7ydpglQFIXf7pobq1+19So/P7Nx4DofvU6LIdbClU
15, 51 -- VZVWGEj2GJcEgsLFbRM87tE1ApgTc0NgJgehP9x3cRNPyL7MadRMUPc5RxXxN8rK054Z
15, 51 -- DY3OSlQnPofAFUGlffFk+p20d5ozQjqCPreEEYxBFePN7Ab1l+ZA38+7Q8WmuZRQyoId
15, 51 -- kzTw==
15, 51 -- X-Gm-Message-State: AG10YORdrEcs3FZT3Oq5rKMD1Hm90RWdjRiU0GXmfQhoQExijOKvsBxasSZzbRifaRWu4g==
15, 51 -- X-Received: by 10.66.141.142 with SMTP id ro14mr5672820pab.112.1455317046561;
15, 51 -- Fri, 12 Feb 2016 14:44:06 -0800 (PST)
15, 51 -- Received: from NestorsrPro2014.home.gateway (123-243-23-38.static.tpgi.com.au. [123.243.23.38])
15, 51 -- by smtp.googlemail.com with ESMTPSA id dz8sm21753261pab.19.2016.02.12.14.44.04
15, 51 -- for {microscopy-at-microscopy.com}
15, 51 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 51 -- Fri, 12 Feb 2016 14:44:05 -0800 (PST)
15, 51 -- Subject: viaWWW:Protocol for embedding rubber in plastic resin
15, 51 -- References: {201602122102.u1CL24l9005652-at-ns.microscopy.com}
15, 51 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 51 -- Reply-To: microscopy.listserver-at-gmail.com
15, 51 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 51 -- X-Forwarded-Message-Id: {201602122102.u1CL24l9005652-at-ns.microscopy.com}
15, 51 -- Message-ID: {56BE6031.7070104-at-gmail.com}
15, 51 -- Date: Sat, 13 Feb 2016 09:44:01 +1100
15, 51 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
15, 51 -- Gecko/20100101 Thunderbird/38.5.1
15, 51 -- MIME-Version: 1.0
15, 51 -- In-Reply-To: {201602122102.u1CL24l9005652-at-ns.microscopy.com}
15, 51 -- Content-Type: text/plain; charset=windows-1252; format=flowed
15, 51 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================

From conversion.seo-at-host2015.lw-china-cdn.com Fri Feb 12 19:53:55 2016
Return-Path: {conversion.seo-at-host2015.lw-china-cdn.com}
Received: from conversion-seo.cn ([61.104.155.170])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1D1roho003819
for {microscopylistserverarchive5-at-microscopy.com} ; Fri, 12 Feb 2016 19:53:53 -0600
DKIM-Signature: v=1; a=rsa-sha1; c=relaxed/relaxed;
d=conversion-seo.cn; s=key; h=Reply-To:From:To:Subject:
MIME-Version:Content-Type:Content-Transfer-Encoding; bh=KrfN6lI6
siHVsxly0fAERDlMRmI=; b=VAEOlG0hjNwzBWh7iwI1vwYacDGM3Ts0vFAwDHXh
ekEdKHTi7LvsmONntnAlWPoLZP0Vu+EFuSb+6rrGMCHV50zduINNqw3wyc1NHVq0
BUzIWCJol81gVSm0910q/vCbKKKytn9pWFCuFqkhMZEoPi88+fy4RLf9oRy1l31x
9Zg=
Reply-To: conversion.seo-at-host2015.lw-china-cdn.com


Quality Social Services to improve web presence
http://www.conversion-seo.cn/social-media-packages/













Unsubscribe Option available on the footer of our website







From: henning.stahlberg-at-unibas.ch
Date: Sat, 13 Feb 2016 04:22:13 -0600
Subject: [Microscopy] ICON 2016 - International Conference on Nanoscopy. Basel, June

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html
by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1DAMK9b024701
for {MicroscopyListserverArchive5-at-microscopy.com} ; Sat, 13 Feb 2016 04:22:20 -0600
Received: (from mail-at-localhost)
by ns.microscopy.com (8.12.11.20060308/8.12.11/Submit) id u1DAMDgv024699;
Sat, 13 Feb 2016 04:22:13 -0600

Dear Colleagues,

We would like to draw your attention to the upcoming conference on super-resolution light microscopy, hosted at the University of Basel in Switzerland on June 7-11, 2016.

Join world-changing thinkers and innovators from the Biophysical and Life Science communities:
Super-resolution imaging methods now can provide spatial resolution that is well below the diffraction limit, approaching virtually molecular resolution. They can be applied to biological samples and provide new and exciting views on the structural organization of cells and the dynamics of biomolecular assemblies on wide timescales. These revolutionary developments come with novel requirements for fluorescent probes, labeling techniques, and data interpretation strategies. Researchers from across the scientific spectrum are involved in designing improved tools and solving previously intractable problems using super-resolution techniques.

We invite you to attend the ,AeoInternational Conference On Nanoscopy 2016,Aeo, which will uniquely and exclusively focus on this topic. Held in Basel, Switzerland 07-10 June 2016, it will bring together an international group of experts to discuss the latest advances and future directions in the field.

Markus Sauer, Conference Chair, University of W/orzburg, Germany

Details and registration are on http://www.icon-europe.org

Speakers include
Eric Betzig (Janelia, USA)
Lothar Schermelleh (Oxford University, UK)
Rainer Heintzmann (University Jena, Germany)
Ingo Gregor (University of G/dttingen, Germany)
Thomas Huser (University Bielefeld, Germany)
Markus Sauer (University W/orzburg, Germany)
Adriaan Houtsmuller (Erasmus MC, Netherlands)
Jonas Ries (EMBL, Germany)
Sjoerd Stallinga (TU Delft, Netherlands)
Melike Lakadamyali (ICFO, Spain)
Suliana Manley (EPFL, Switzerland)
Joerg Bewersdorf (Yale, USA)
Katrin Willig (MPI EM, Germany)
Christian Eggeling (Oxford University, UK)
Ilaria Testa (KTH, Sweden)
Martin Booth (Oxford University, UK)
Erik Manders (UvA, Netherlands)
Edoardo Charbon (TU Delft, Netherlands)
Oliver Biehlmaier (University Basel, Switzerland)
and others.

Another 12 presentations will be selected from submitted participant abstracts.

On behalf of the organizing committee,
Gregor Drummen, Oliver Biehlmaier, Henning Stahlberg, and Manuela Holzer

with best greetings,
Henning.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



==============================Original Headers==============================
12, 40 -- From henning.stahlberg-at-unibas.ch Sat Feb 13 04:22:08 2016
12, 40 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165])
12, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1DALurG024691
12, 40 -- for {Microscopy-at-microscopy.com} ; Sat, 13 Feb 2016 04:21:59 -0600
12, 40 -- Received: from localhost (localhost [127.0.0.1])
12, 40 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 19D5E220164;
12, 40 -- Sat, 13 Feb 2016 11:21:39 +0100 (CET)
12, 40 -- X-Virus-Scanned: amavisd-new at unibas.ch
12, 40 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165])
12, 40 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024)
12, 40 -- with LMTP id KA5rUja9CUoh; Sat, 13 Feb 2016 11:21:39 +0100 (CET)
12, 40 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131])
12, 40 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 097BE22015E;
12, 40 -- Sat, 13 Feb 2016 11:21:39 +0100 (CET)
12, 40 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by
12, 40 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id
12, 40 -- 14.03.0266.001; Sat, 13 Feb 2016 11:21:38 +0100
12, 40 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
12, 40 -- To: Microscopy {Microscopy-at-microscopy.com}
12, 40 -- CC: Gregor Drummen {gpcdrummen-at-bionano-solutions.de} ,
12, 40 -- Oliver Biehlmaier
12, 40 -- {oliver.biehlmaier-at-unibas.ch} ,
12, 40 -- Manuela Holzer {manuela.holzer-at-unibas.ch}
12, 40 -- Subject: ICON 2016 - International Conference on Nanoscopy. Basel, June
12, 40 -- 7-11, 2016. Registration now open.
12, 40 -- Thread-Topic: ICON 2016 - International Conference on Nanoscopy. Basel, June
12, 40 -- 7-11, 2016. Registration now open.
12, 40 -- Thread-Index: AQHRZkhS+E7QZ+G7aE25OZYrqmR1Rw==
12, 40 -- Date: Sat, 13 Feb 2016 10:21:38 +0000
12, 40 -- Message-ID: {23434275-3A85-46D6-8712-52E4A27A09B6-at-unibas.ch}
12, 40 -- Accept-Language: en-US, de-CH
12, 40 -- Content-Language: en-US
12, 40 -- X-MS-Has-Attach:
12, 40 -- X-MS-TNEF-Correlator:
12, 40 -- x-originating-ip: [131.152.226.242]
12, 40 -- Content-Type: text/plain; charset="utf-8"
12, 40 -- Content-ID: {31B4E6794420F14FA05CBC4CD0A31CC4-at-zuv.ads.unibas.ch}
12, 40 -- MIME-Version: 1.0
12, 40 -- Content-Transfer-Encoding: 8bit
12, 40 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1DALurG024691
==============================End of - Headers==============================




From: johndmcmaster-at-gmail.com
Date: Sun, 14 Feb 2016 23:58:53 -0600
Subject: [Microscopy] Wanted: Technics Hummer II manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Trying to fix one up for personal use. Would be very interested if
someone has a manual or can direct me where I might find one. I'm
currently following a lead with Pumping Station 1 but looking at other
options in case I don't hear back. Thanks!

John


==============================Original Headers==============================
3, 46 -- From johndmcmaster-at-gmail.com Sun Feb 14 23:58:53 2016
3, 46 -- Received: from mail-pa0-f42.google.com (mail-pa0-f42.google.com [209.85.220.42])
3, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1F5wqvm018810
3, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Feb 2016 23:58:53 -0600
3, 46 -- Received: by mail-pa0-f42.google.com with SMTP id fy10so40839592pac.1
3, 46 -- for {Microscopy-at-microscopy.com} ; Sun, 14 Feb 2016 21:58:39 -0800 (PST)
3, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 46 -- d=gmail.com; s=20120113;
3, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version
3, 46 -- :content-type:content-transfer-encoding;
3, 46 -- bh=YbyWQO2HBy9fF4OCLd6NtFtmMVdarZaqOrQofgfF8yk=;
3, 46 -- b=y2DEAnwRQUR1Ay10UX18b7d4moUB2PGi+CYaqjanIjcpqm6OltC7XYJEVda4AFCuK1
3, 46 -- n3VlbKcncu+Mjp7tQoTroTyrt2O3MsAgCjrapZM6gORHd0W2m87GHcpEA8uhkP+J2i4b
3, 46 -- jpfTra8XrYpGONpsm26HR3cBRb7yfo9pThzRDDxvxo2F1YM8pvAYws/ezgF4IvbwnAk3
3, 46 -- TZiP/R00Yaixrn4Mbj/dqJ/rmFld/+ArvBf8AMiOHqr4BBsJeXH2IldEcuj1uXS16Mku
3, 46 -- IimMk9Ck+rG+oJTson0DY7pp/KQMz1XpK4aeYf2gwqCbws0SVcNxJwUagL0ov1lFkE54
3, 46 -- kpiQ==
3, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 46 -- d=1e100.net; s=20130820;
3, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent
3, 46 -- :mime-version:content-type:content-transfer-encoding;
3, 46 -- bh=YbyWQO2HBy9fF4OCLd6NtFtmMVdarZaqOrQofgfF8yk=;
3, 46 -- b=AZ7MHceWb7ouFp2CqIRby8GWD6ZQGsYy6bDiqtSWgB4pEtTYEdG+z757YhmL1wLu8f
3, 46 -- 6rnJ6qmG+0ho6ar880oaYShAhdlYQ8fJRXK2JmdU1gD48S8HfVfHJvNXOFWw2iFbocvF
3, 46 -- oMZc2Kcb0v7Usop68w27Ggw9Q2ZPnUbU4UXLg3mWxvoGwxIQxMV7ffu+r0WF/8Bsl4XT
3, 46 -- w7xhXCgdHk8pItzLpI9MxNiOh67tpHzY1vpzSzm+ZfbnVQ7W/2m4FpJS7xhQ6leVErd0
3, 46 -- HsTSt4B8A3KCTD5eE0t2sswbJFJqP8ayH+Z+vBCJud0LtddXJ9Qvhmg0aGvGK3wVs/au
3, 46 -- PE0w==
3, 46 -- X-Gm-Message-State: AG10YOQliphydnnz9UHSs0/Kt3LS4KorgkuOJFJ2+exiWH5H5tD/XdbgIc5n0ip4zr9tWw==
3, 46 -- X-Received: by 10.66.219.71 with SMTP id pm7mr20818929pac.137.1455515919016;
3, 46 -- Sun, 14 Feb 2016 21:58:39 -0800 (PST)
3, 46 -- Received: from [192.168.4.218] (c-24-6-49-99.hsd1.ca.comcast.net. [24.6.49.99])
3, 46 -- by smtp.googlemail.com with ESMTPSA id k14sm35302959pfj.0.2016.02.14.21.58.38
3, 46 -- for {Microscopy-at-microscopy.com}
3, 46 -- (version=TLSv1/SSLv3 cipher=OTHER);
3, 46 -- Sun, 14 Feb 2016 21:58:38 -0800 (PST)
3, 46 -- To: Microscopy-at-microscopy.com
3, 46 -- From: John McMaster {johndmcmaster-at-gmail.com}
3, 46 -- Subject: Wanted: Technics Hummer II manual
3, 46 -- Message-ID: {56C1690D.7050304-at-gmail.com}
3, 46 -- Date: Sun, 14 Feb 2016 21:58:37 -0800
3, 46 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101
3, 46 -- Thunderbird/38.5.1
3, 46 -- MIME-Version: 1.0
3, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed
3, 46 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================





From: microscopy.listserver-at-gmail.com
Date: Tue, 16 Feb 2016 09:00:28 -0600
Subject: [Microscopy] viaWWW:11th Annual Confocal Microscopy Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: bob.price-at-uscmed.sc.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both bob.price-at-uscmed.sc.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: bob.price-at-uscmed.sc.edu
Name: Bob Price

Organization: Univ South Carolina

Title-Subject: [Filtered] 11th Annual Confocal Microscopy Workshop

Message: The South Carolina INBRE Program and the USC School of Medicine Instrumentation Resource
Facility are pleased to announce the 11th annual workshop on Basic Confocal Microscopy. The Workshop
will be held June 13-17, 2016 at the USC School of Medicine in Columbia, SC.

Workshop material is directed towards beginning and intermediate users of confocal microscopes and
involves a series of lectures (specimen preparation, labeling strategies, proper set-up of
instrument operating parameters, proper handling of 2D and 3D confocal images in programs such as
Adobe Photoshop and AMIRA), hands on specimen preparation, and time on a number of different point
scanning and spinning disk confocal microscopes.

Companies that have provided equipment and applications experts for past workshops include Leica,
Nikon, Olympus, Perkin Elmer, Zeiss, Intelligent Imaging Innovations (3i) and Photometrix.
Participants will have ample time for hands on use of the instruments and processing of images in
Photoshop and AMIRA during the workshop.

Faculty will include Dr. Jay Jerome of Vanderbilt University, Dr. Ralph Albrecht of the University
of Wisconsin Madison, Dr. John Mackenzie of North Carolina State University, Dr. Tom Trusk of the
Medical University of South Carolina, and Dr. Bob Price of the University of South Carolina.

The $350 tuition covers supplies for processing of specimens, breakfast and lunch for the week, 2
dinners and a reception on Lake Murray.

For registration information please see: http://irf.med.sc.edu/ or contact Anna Harper
(Anna.Harper-at-uscmed.sc.edu) or Bob Price (bob.price-at-uscmed.sc.edu)


Bob Price
Tel: 803-216-3824
Admin Asst Tel: 803-216-3825
Dept Cell Biology and Anatomy
School of Medicine
Univ South Carolina
6439 Garner's Ferry Road
Columbia, SC 29208


Login Host: 129.252.69.40
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
20, 53 -- From microscopy.listserver-at-gmail.com Tue Feb 16 09:00:28 2016
20, 53 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176])
20, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1GF0Sgt010580
20, 53 -- for {microscopy-at-microscopy.com} ; Tue, 16 Feb 2016 09:00:28 -0600
20, 53 -- Received: by mail-ig0-f176.google.com with SMTP id g6so32916762igt.1
20, 53 -- for {microscopy-at-microscopy.com} ; Tue, 16 Feb 2016 07:00:14 -0800 (PST)
20, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 53 -- d=gmail.com; s=20120113;
20, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
20, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
20, 53 -- bh=+lt4NrS1WueJgWCcWfVTyWcKbjORRIEFoRmSARFhQOQ=;
20, 53 -- b=uxu1P2ttNeGhAQ1Tf5OCXGccD80LbqlejhMy53FD72V1ZM2rrjKh8zmcemvHWovr/l
20, 53 -- z3JM6TACGt/TD4svUA99EG8EHw9jVR0QAcEWlnRhXHAHEC0j3ZHiVs3Bhqdk/kYiL4tt
20, 53 -- e8wIz2obBMN5ZAVPl6mfB2YHbp+5zdpvht4YinVMQVX6jV1hbwpvVxxud7ArNex5eS4M
20, 53 -- wIEBbi9tFajM2mIJLGduIxwdZbBSqhauzoLyKfZsI1fvC0x5E6JGTXMdUJbNGFTbAP14
20, 53 -- L+6PGoC2rXri/oEz3AOloHtyfDo4ynIUCyyw18RjpkPOea3Q6taYwSi/+w2fPC9Z2qw4
20, 53 -- JjSA==
20, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 53 -- d=1e100.net; s=20130820;
20, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
20, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
20, 53 -- :content-transfer-encoding;
20, 53 -- bh=+lt4NrS1WueJgWCcWfVTyWcKbjORRIEFoRmSARFhQOQ=;
20, 53 -- b=gjRfb3v7lncLsn7SeoTPZfAkA0MRpZrBtSBsyXstLkefgwlhKHBzfk63M6+2wlFiiq
20, 53 -- B7tiK211fRkrfuCZciyRGeeBIsjgbxisJA39HIu7iRIKbxm+3e0GEWaVNlQ8+1UikhU9
20, 53 -- eugrxxNAyf4usKqtxPPuv3pofdVLYDv1tIex0FVyZVZ9acCJlkJK0U86rwkmmkG3NuhE
20, 53 -- Il5+y6uYB38XILfc5RY2N/AGSqAGvI//RazI89d1rTPfKKfhy1mog1cfxEZ5K/XTNwm3
20, 53 -- kAkHL5qBX6XviMewjW4vATUZZHnuVj/nN9uygBB61Byz8Vtc4fUlDHEBR0ifGKyD70fL
20, 53 -- BvPA==
20, 53 -- X-Gm-Message-State: AG10YOS2bEJ3fgIZYfXYpBY374/FKXB57xuJQqXTqLoRA2IfvlZg3u0eOVsZfCHzulbqlg==
20, 53 -- X-Received: by 10.50.160.7 with SMTP id xg7mr17471804igb.64.1455634814053;
20, 53 -- Tue, 16 Feb 2016 07:00:14 -0800 (PST)
20, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
20, 53 -- by smtp.googlemail.com with ESMTPSA id n1sm8707876ige.19.2016.02.16.07.00.12
20, 53 -- for {microscopy-at-microscopy.com}
20, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
20, 53 -- Tue, 16 Feb 2016 07:00:13 -0800 (PST)
20, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
20, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
20, 53 -- {microscopylistserver-noreply-at-microscopy.com}
20, 53 -- Subject: viaWWW:11th Annual Confocal Microscopy Workshop
20, 53 -- References: {201602161447.u1GElOQl010080-at-ns.microscopy.com}
20, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
20, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
20, 53 -- X-Forwarded-Message-Id: {201602161447.u1GElOQl010080-at-ns.microscopy.com}
20, 53 -- Message-ID: {56C3397C.2020101-at-microscopy.com}
20, 53 -- Date: Tue, 16 Feb 2016 09:00:12 -0600
20, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
20, 53 -- Gecko/20100101 Thunderbird/38.5.1
20, 53 -- MIME-Version: 1.0
20, 53 -- In-Reply-To: {201602161447.u1GElOQl010080-at-ns.microscopy.com}
20, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
20, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: bicarbaj-at-mtholyoke.edu
Date: Tue, 16 Feb 2016 14:18:28 -0600
Subject: [Microscopy] LM-Experiences with AmScope?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I am in the process of preparing a grant and I am considering adding
about a dozen compound microscopes with phase contrast capabilities
4x-100x objectives. These microscopes would be used in undergraduate
teaching labs, so high resolution is unnecessary.

I am unfamiliar with the quality of AmScope microscopes but they have
what looks like a decent scope (Model B690B-PL-PCT200INF). We
currently have an army of Olympus BH-2s but the number of biology
majors has increased significantly, so we are looking for some
affordable options.

Does anyone have experience with these microscopes? Any insight would
be greatly appreciated!

Thank you,

-Blanca

-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

==============================Original Headers==============================
7, 38 -- From bicarbaj-at-mtholyoke.edu Tue Feb 16 14:18:28 2016
7, 38 -- Received: from mail-wm0-f49.google.com (mail-wm0-f49.google.com [74.125.82.49])
7, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1GKIRtb014779
7, 38 -- for {microscopy-at-microscopy.com} ; Tue, 16 Feb 2016 14:18:28 -0600
7, 38 -- Received: by mail-wm0-f49.google.com with SMTP id g62so126746188wme.1
7, 38 -- for {microscopy-at-microscopy.com} ; Tue, 16 Feb 2016 12:18:14 -0800 (PST)
7, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 38 -- d=mtholyoke-edu.20150623.gappssmtp.com; s=20150623;
7, 38 -- h=mime-version:date:message-id:subject:from:to:content-type;
7, 38 -- bh=3q5W4n0Xdza97eRKJ8RTljCakABiqayFwq07fVg4A1s=;
7, 38 -- b=BMyjbLRTCprN6E/3mUd/h+oLfUgr1anWp3F8ej5E494xMCb1d5x1cZmDRLmp5CNyIu
7, 38 -- waQGbWfVcjNJXSMg5Lp7/K3aBLqzC4o7LF3znFIlXf73D6QGShGX3FheCja3yKb81od4
7, 38 -- AgKe93VUnTCFCtbczRhNOLAMMlnwo6oyYCDs0N4W4M9K5TPn2RANAQ8mRmCNVESaIHCe
7, 38 -- ZDhGfPyCTj/FHSZc6TgKUQYQJIo3ZA9ueIbK7xGzZw2liTWfVcVQAqfh548LWhiqXNSe
7, 38 -- YcVsbqZxflejrzRrqZzZlPW/3cz5X9pPGa0Sd30N2PlHlSO7XkSh/Q6mtlA0npVnq9d2
7, 38 -- 19OQ==
7, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 38 -- d=1e100.net; s=20130820;
7, 38 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to
7, 38 -- :content-type;
7, 38 -- bh=3q5W4n0Xdza97eRKJ8RTljCakABiqayFwq07fVg4A1s=;
7, 38 -- b=fIxfIIyKCWblNWx5M4Mxe4Z6v4oy47Ey9g9eVaYfq0HEGuYqJj6Nl6uQat2mitkEes
7, 38 -- QXxk4fqJK7nP/SFOrqIcfYGJbPw2GM4YY/xGjFh8QnHu9ayZSFBU01cmnFaWDo+KL7eh
7, 38 -- sgHXRKXeB/RZ33D6xRn9vME5lR1VqG1OxvT9aottuDmenmuxfvV5Sic3ee3a2+Sb/+2m
7, 38 -- uWtAz/CTB+bYGLM3OsNoKGB3bTGGrh3qYHmj0Q8tM8cQL6BkXndXQFpLnYuQvjbGy9FY
7, 38 -- +iX7zKsvucE2fXdywBaVRA8JzGRnA6PbeaSf4vxGDNEaX1MKYWST1HK4KqlnFKUDXUWd
7, 38 -- sfdw==
7, 38 -- X-Gm-Message-State: AG10YORy3ulwHMi6eki3hZq/pPriaSRsBMypd3mE96CTVxT8l6FVv5Ky60B6YgZppx7F99q7EXr687WY335v6Irz
7, 38 -- MIME-Version: 1.0
7, 38 -- X-Received: by 10.28.186.11 with SMTP id k11mr19963149wmf.79.1455653893637;
7, 38 -- Tue, 16 Feb 2016 12:18:13 -0800 (PST)
7, 38 -- Received: by 10.28.113.201 with HTTP; Tue, 16 Feb 2016 12:18:13 -0800 (PST)
7, 38 -- Date: Tue, 16 Feb 2016 15:18:13 -0500
7, 38 -- Message-ID: {CAO94sukjT829HaL-tb7dFBdygZY7qnhyM2QX8r+e044-UbiUqg-at-mail.gmail.com}
7, 38 -- Subject: LM-Experiences with AmScope?
7, 38 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu}
7, 38 -- To: microscopy-at-microscopy.com
7, 38 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================







From: stefan.diller-at-t-online.de
Date: Wed, 17 Feb 2016 06:58:55 -0600
Subject: [Microscopy] Jeol JSM 5900LV manual and remotable functions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

anybody ou there who can help me out with an operation manual in PDF on the 5900?
I am also looking for a manual concerning the functions which can be remoted on the 5900. Does anybody have experience doing this?
What SEM parameters can be read / written...?


Thanks,
Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


==============================Original Headers==============================
9, 22 -- From stefan.diller-at-t-online.de Wed Feb 17 06:58:55 2016
9, 22 -- Received: from mailout02.t-online.de (mailout02.t-online.de [194.25.134.17])
9, 22 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1HCwsYQ017731
9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 17 Feb 2016 06:58:54 -0600
9, 22 -- Received: from fwd03.aul.t-online.de (fwd03.aul.t-online.de [172.20.27.148])
9, 22 -- by mailout02.t-online.de (Postfix) with SMTP id D976F2F2451
9, 22 -- for {microscopy-at-microscopy.com} ; Wed, 17 Feb 2016 13:58:39 +0100 (CET)
9, 22 -- Received: from mac-pro.fritz.box (bRhnokZTQhNsANNyUQ660I+EDWQPZpu8ls6DdP4mBlMxzQvbAGbof+lUJJbGza3gH8-at-[84.174.126.42]) by fwd03.t-online.de
9, 22 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted)
9, 22 -- esmtp id 1aW1gc-0afHJg0; Wed, 17 Feb 2016 13:58:30 +0100
9, 22 -- To: microscopy-at-microscopy.com
9, 22 -- From: Stefan Diller {stefan.diller-at-t-online.de}
9, 22 -- Subject: Jeol JSM 5900LV manual and remotable functions
9, 22 -- Message-ID: {56C46E75.7010202-at-t-online.de}
9, 22 -- Date: Wed, 17 Feb 2016 13:58:29 +0100
9, 22 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
9, 22 -- Gecko/20100101 Thunderbird/38.5.1
9, 22 -- MIME-Version: 1.0
9, 22 -- Content-Type: text/plain; charset=windows-1252; format=flowed
9, 22 -- Content-Transfer-Encoding: 7bit
9, 22 -- X-ID: bRhnokZTQhNsANNyUQ660I+EDWQPZpu8ls6DdP4mBlMxzQvbAGbof+lUJJbGza3gH8
9, 22 -- X-TOI-MSGID: 77ed59ca-72e2-4c02-b7fb-9d5f98c87b55
==============================End of - Headers==============================




From: tkremer-at-ipstesting.com
Date: Wed, 17 Feb 2016 09:39:15 -0600
Subject: [Microscopy] SEM with EDS Comparing the new benchtop desktop instruments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

Do any of you have experience with the newer benchtop or desktop SEM's? They seem quite capable and their cost to purchase and maintain, compared to the full scale SEM's, looks attractive. Their advertised performance is appealing. Do they maintain that performance? Do they require much service? Should I have a service contract? Do the accessories function well (e.g. rotating and tilting stage/holder, charge reduction holder)? Is the provided EDS system comprehensive and reliable? Your replies would be greatly appreciated.

Tom Kremer
tkremer-at-ipstesting.com


==============================Original Headers==============================
4, 49 -- From tkremer-at-ipstesting.com Wed Feb 17 09:39:15 2016
4, 49 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0089.outbound.protection.outlook.com [207.46.100.89])
4, 49 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1HFdF5D011285
4, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 17 Feb 2016 09:39:15 -0600
4, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=ipstesting.com;
4, 49 -- s=selector1; h=From:To:Date:Subject:Message-ID:Content-Type:MIME-Version;
4, 49 -- bh=VdDr4bUQN8m0btp8w4uzLQA45YNYDbgXt2ozzCkUZ2k=;
4, 49 -- b=N33TVBRZ2pA3MFCmJna4eJFnn+LLYBgGmwZa6KTJtq26U7UOIuupjRrcTi3c0TyCI6n3xyIHXpBG0t/Y/Wn/a8kIwuVemVnaMWZSXnS1Ex4clLxTwwuMDI6IWrFdWav8VreiCehu3yZNFj45GkEehKZ9zAi8hL9uPEMgnVOM0N8=
4, 49 -- Received: from BN1PR07MB389.namprd07.prod.outlook.com (10.141.58.141) by
4, 49 -- BN1PR07MB392.namprd07.prod.outlook.com (10.141.58.151) with Microsoft SMTP
4, 49 -- Server (TLS) id 15.1.403.16; Wed, 17 Feb 2016 15:39:00 +0000
4, 49 -- Received: from BN1PR07MB389.namprd07.prod.outlook.com ([169.254.6.115]) by
4, 49 -- BN1PR07MB389.namprd07.prod.outlook.com ([169.254.6.115]) with mapi id
4, 49 -- 15.01.0409.017; Wed, 17 Feb 2016 15:38:59 +0000
4, 49 -- From: Tom Kremer {tkremer-at-ipstesting.com}
4, 49 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
4, 49 -- Subject: SEM with EDS Comparing the new benchtop desktop instruments
4, 49 -- Thread-Topic: SEM with EDS Comparing the new benchtop desktop instruments
4, 49 -- Thread-Index: AdFpmVC8JLl6k7olThyW8RmBOWZLAw==
4, 49 -- Date: Wed, 17 Feb 2016 15:38:59 +0000
4, 49 -- Message-ID: {BN1PR07MB389AA742ACB826C23947175DBAE0-at-BN1PR07MB389.namprd07.prod.outlook.com}
4, 49 -- Accept-Language: en-US
4, 49 -- Content-Language: en-US
4, 49 -- X-MS-Has-Attach:
4, 49 -- X-MS-TNEF-Correlator:
4, 49 -- authentication-results: microscopy.com; dkim=none (message not signed)
4, 49 -- header.d=none;microscopy.com; dmarc=none action=none
4, 49 -- header.from=ipstesting.com;
4, 49 -- x-originating-ip: [96.11.14.178]
4, 49 -- x-microsoft-exchange-diagnostics: 1;BN1PR07MB392;5:G8+PY0J2GNNl8YzY/4J/6m/QzJ+lvK79Zudi6lHgOY8LMKDPViODaTPuhu9MCE/kfe5l2R7WX0OWH+t5rEfNkUll3P07lfJxfBBcst10ZNIcPQk24Qnt1Uooq/Sx+nouq/bSkrw6AskKMP5abIuw9w==;24:YTPJyYYh4Aeab+DeGjHe/ZXeQAnJjxjtmFbcsq8KuFmdQR5JLPVgVbJ2DmplFbhuH27/WQ4/8bhlp8O7id2GbyY07mulSdPROQZ49W4Qm40=
4, 49 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BN1PR07MB392;
4, 49 -- x-ms-office365-filtering-correlation-id: ce3d9565-3cf6-4da1-785c-08d337b0748a
4, 49 -- x-microsoft-antispam-prvs: {BN1PR07MB392B77C4215F666C8D74AE3DBAE0-at-BN1PR07MB392.namprd07.prod.outlook.com}
4, 49 -- x-exchange-antispam-report-test: UriScan:;
4, 49 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046);SRVR:BN1PR07MB392;BCL:0;PCL:0;RULEID:;SRVR:BN1PR07MB392;
4, 49 -- x-forefront-prvs: 085551F5A8
4, 49 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(53754006)(122556002)(19580405001)(5002640100001)(2900100001)(92566002)(3280700002)(66066001)(33656002)(99286002)(5003600100002)(19580395003)(2906002)(3660700001)(5004730100002)(5008740100001)(229853001)(450100001)(2351001)(10400500002)(1096002)(189998001)(54356999)(50986999)(6116002)(102836003)(1220700001)(87936001)(110136002)(3846002)(40100003)(86362001)(74316001)(107886002)(5001960100002)(76576001)(586003)(2501003);DIR:OUT;SFP:1101;SCL:1;SRVR:BN1PR07MB392;H:BN1PR07MB389.namprd07.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en;
4, 49 -- spamdiagnosticoutput: 1:23
4, 49 -- spamdiagnosticmetadata: NSPM
4, 49 -- Content-Type: text/plain; charset="us-ascii"
4, 49 -- MIME-Version: 1.0
4, 49 -- X-OriginatorOrg: ipstesting.com
4, 49 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 17 Feb 2016 15:38:59.4631
4, 49 -- (UTC)
4, 49 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted
4, 49 -- X-MS-Exchange-CrossTenant-id: fcd656df-08de-421e-a3c5-e640523dfad4
4, 49 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BN1PR07MB392
4, 49 -- Content-Transfer-Encoding: 8bit
4, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1HFdF5D011285
==============================End of - Headers==============================




From: nizets2-at-yahoo.com
Date: Thu, 18 Feb 2016 04:08:19 -0600
Subject: [Microscopy] bias and gain correction on TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining.
Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)?
I can't remember the last time I did it but it is a long time ago.
I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals.
Many thanks in advance.

Regards
Stephane

==============================Original Headers==============================
3, 29 -- From nizets2-at-yahoo.com Thu Feb 18 04:08:18 2016
3, 29 -- Received: from nm14-vm0.bullet.mail.bf1.yahoo.com (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164])
3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IA8HGp022220
3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:08:17 -0600
3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw==
3, 29 -- Received: from [98.139.215.143] by nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
3, 29 -- Received: from [98.139.212.220] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
3, 29 -- X-Yahoo-Newman-Property: ymail-3
3, 29 -- X-Yahoo-Newman-Id: 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com
3, 29 -- X-YMail-OSG: 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka
3, 29 -- 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv
3, 29 -- U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj
3, 29 -- 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ
3, 29 -- m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f
3, 29 -- dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D
3, 29 -- GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT
3, 29 -- XNnKGXA--
3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb 2016 10:08:03 +0000
3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 (UTC)
3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com}
3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
3, 29 -- To: {microscopy-at-microscopy.com}
3, 29 -- Message-ID: {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com}
3, 29 -- Subject: bias and gain correction on TEM cameras
3, 29 -- MIME-Version: 1.0
3, 29 -- Content-Type: text/plain; charset=UTF-8
3, 29 -- Content-Transfer-Encoding: 7bit
3, 29 -- References: {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com}
==============================End of - Headers==============================




From: stefan.diller-at-t-online.de
Date: Thu, 18 Feb 2016 04:40:43 -0600
Subject: [Microscopy] Re: bias and gain correction on TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Stephane,
since the blacklevel ("noise") and gain images are a necessary step for getting an artefact-free and evenly background I think you
should do this short procedure (depends what version of Analysis or ITEM software you are using) often.
When I am installing or updating cameras at the customerYens site I ask for the most used magnfication / spotsize / apertures
setting they use and do the correction images with this setting.
But: I also know a customer who is doing these image sets prior to each high-res image he uses for publication (makes sense ;-) ).

Best wishes,
Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 18.02.16 um 11:27 schrieb nizets2-at-yahoo.com:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} we have a SIS Megaview III camera (SIS, then Olympus and now I see it is EMSIS "again") for over 10 years, a real workhorse, always performing well without complaining.
} Because it works so well I tend to forget about it and now I am just wondering if I need to update the gain and bias corrections (the correction pictures)?
} I can't remember the last time I did it but it is a long time ago.
} I know that it is not a lot of work to do it, I just wondered if there is any reason to do this regularly and if yes, what are the time intervals.
} Many thanks in advance.
}
} Regards
} Stephane
}
} ==============================Original Headers==============================
} 3, 29 -- From nizets2-at-yahoo.com Thu Feb 18 04:08:18 2016
} 3, 29 -- Received: from nm14-vm0.bullet.mail.bf1.yahoo.com (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164])
} 3, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IA8HGp022220
} 3, 29 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:08:17 -0600
} 3, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083; bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw==
} 3, 29 -- Received: from [98.139.215.143] by nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
} 3, 29 -- Received: from [98.139.212.220] by tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
} 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
} 3, 29 -- X-Yahoo-Newman-Property: ymail-3
} 3, 29 -- X-Yahoo-Newman-Id: 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com
} 3, 29 -- X-YMail-OSG: 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka
} 3, 29 -- 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv
} 3, 29 -- U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj
} 3, 29 -- 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ
} 3, 29 -- m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f
} 3, 29 -- dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D
} 3, 29 -- GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT
} 3, 29 -- XNnKGXA--
} 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb 2016 10:08:03 +0000
} 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000 (UTC)
} 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 3, 29 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
} 3, 29 -- To: {microscopy-at-microscopy.com}
} 3, 29 -- Message-ID: {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com}
} 3, 29 -- Subject: bias and gain correction on TEM cameras
} 3, 29 -- MIME-Version: 1.0
} 3, 29 -- Content-Type: text/plain; charset=UTF-8
} 3, 29 -- Content-Transfer-Encoding: 7bit
} 3, 29 -- References: {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com}
} ==============================End of - Headers==============================
}




==============================Original Headers==============================
8, 25 -- From stefan.diller-at-t-online.de Thu Feb 18 04:40:43 2016
8, 25 -- Received: from mailout01.t-online.de (mailout01.t-online.de [194.25.134.80])
8, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IAefMr004533
8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 04:40:42 -0600
8, 25 -- Received: from fwd11.aul.t-online.de (fwd11.aul.t-online.de [172.20.27.152])
8, 25 -- by mailout01.t-online.de (Postfix) with SMTP id 3A8F9427EE
8, 25 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 11:40:27 +0100 (CET)
8, 25 -- Received: from mac-pro.fritz.box (ESUE6aZ-whYwAfSldbPQXTZ55PEafbWxBPK4eG+X+EOX9+4smi90B-sXFMUQ+k6wwJ-at-[91.58.167.160]) by fwd11.t-online.de
8, 25 -- with (TLSv1.2:ECDHE-RSA-AES256-SHA encrypted)
8, 25 -- esmtp id 1aWM0T-1m0f680; Thu, 18 Feb 2016 11:40:21 +0100
8, 25 -- Subject: Re: [Microscopy] bias and gain correction on TEM cameras
8, 25 -- To: microscopy-at-microscopy.com
8, 25 -- References: {201602181027.u1IARO2S027682-at-ns.microscopy.com}
8, 25 -- From: Stefan Diller {stefan.diller-at-t-online.de}
8, 25 -- Message-ID: {56C59F94.3010105-at-t-online.de}
8, 25 -- Date: Thu, 18 Feb 2016 11:40:20 +0100
8, 25 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
8, 25 -- Gecko/20100101 Thunderbird/38.6.0
8, 25 -- MIME-Version: 1.0
8, 25 -- In-Reply-To: {201602181027.u1IARO2S027682-at-ns.microscopy.com}
8, 25 -- Content-Type: text/plain; charset=windows-1252; format=flowed
8, 25 -- X-ID: ESUE6aZ-whYwAfSldbPQXTZ55PEafbWxBPK4eG+X+EOX9+4smi90B-sXFMUQ+k6wwJ
8, 25 -- X-TOI-MSGID: a7f85412-8967-40de-94d5-f69dc661289f
8, 25 -- Content-Transfer-Encoding: 8bit
8, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IAefMr004533
==============================End of - Headers==============================




From: benada-at-biomed.cas.cz
Date: Thu, 18 Feb 2016 05:39:26 -0600
Subject: [Microscopy] Re: bias and gain correction on TEM cameras

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephane,
We have SIS MegaView II 16 years old, even older then yours Megaview III.
Well, we used to record the correction images quite frequently, approximately once per month or two. As the ccd chip and scintillator were getting older, the interval for "shading correction" adjustment had to be shorter. After 15 years, we had to record every week a new set of gain and bias images.

You can quite easily check whether you need to perform new adjustment of "shading correction" or not.
1. Take-off the sample holder out of the column
2. Adjust the illumination on the screen
3. Insert camera
4. Adjust beam intensity to ~50%
5. Take an image.
Now measure the histogram of "Gray value distribution" in the recorded image (Main manu: Measure -} Histogram). Look at the course of the histogram line. If it is smooth and the distribution is narrow (~100 for MegaVieew II is OK) then you do not need to adjust "shading correction". Otherwise you should take a new set of gain and bias images.

My best regards

Oldrich


--
Old=oich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
V/ {} de=ask/* 1083
142 20 Prague 4
Czech Republic

On Thu, 18 Feb 2016 04:19:53 -0600, nizets2-at-yahoo.com wrote :
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of
} America To Subscribe/Unsubscribe --
} http://www.microscopy.com/MicroscopyListserver On-Line Help
} http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear Listers,
}
} we have a SIS Megaview III camera (SIS, then Olympus and now I see it
} is EMSIS "again") for over 10 years, a real workhorse, always
} performing well without complaining. Because it works so well I tend
} to forget about it and now I am just wondering if I need to update
} the gain and bias corrections (the correction pictures)? I can't
} remember the last time I did it but it is a long time ago. I know
} that it is not a lot of work to do it, I just wondered if there is
} any reason to do this regularly and if yes, what are the time
} intervals. Many thanks in advance.
}
} Regards
} Stephane
}
} ==============================Original
} Headers============================== 3, 29 -- From nizets2-at-yahoo.com
} Thu Feb 18 04:08:18 2016 3, 29 -- Received: from
} nm14-vm0.bullet.mail.bf1.yahoo.com
} (nm14-vm0.bullet.mail.bf1.yahoo.com [98.139.213.164]) 3, 29 --
} by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
} u1IA8HGp022220 3, 29 -- for {microscopy-at-microscopy.com} ; Thu,
} 18 Feb 2016 04:08:17 -0600 3, 29 -- DKIM-Signature: v=1;
} a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455790083;
} bh=x7ccEl93SQjy6RmTa7YGFTI9AoSGhaKtVxThqGCF8uI=;
} h=Date:From:Reply-To:To:Subject:References:From:Subject;
} b=mxmCwIJ35ltTrPD3kub/zVxqaafRcOAxfgm6ezzoJrp1pUCYmcfPSQRcz2UZCYB7E2VDYutG4Acwoc6fb+dgYjGb/qn0SYRRjmHssI4OgoU5KzEsVT7z/hXuvkawJQrdxcMBZFjaSBsgwQW/NqldgKsdDF7QxPC3nogGqNgnXcC8R6tuyu5iftY8Mv4hEEhxZBqjkVbYVTJD4II/3DuBYpGu2M8zgAudq87whg7+Fz8BK1DwDQbxqX6RVf7LeWJ6wCuaLj+MlbtnyWJgVCLjFByywNR82D973IPAXq8MANbIjz9vlXDO0aK/VOoD3HTLmooRkrHVEPifDqHg+3oWrw==
} 3, 29 -- Received: from [98.139.215.143] by
} nm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
} 3, 29 -- Received: from [98.139.212.220] by
} tm14.bullet.mail.bf1.yahoo.com with NNFMP; 18 Feb 2016 10:08:03 -0000
} 3, 29 -- Received: from [127.0.0.1] by omp1029.mail.bf1.yahoo.com
} with NNFMP; 18 Feb 2016 10:08:03 -0000 3, 29 --
} X-Yahoo-Newman-Property: ymail-3 3, 29 -- X-Yahoo-Newman-Id:
} 741770.20773.bm-at-omp1029.mail.bf1.yahoo.com 3, 29 -- X-YMail-OSG:
} 9nq693UVM1nU.BaDUcqJJgdhtWG7s9wmJYCi2MeGsNslachC50ErOqvae9Gx8Ka 3, 29
} --
} 9zbrt5rq.gYAwbe3oLBiRmOMyG.BYkEej7Am1ukFXfhrPI_nmsIA4VEGAF51eLmld4zOtlbFAkFv
} 3, 29 --
} U0L7c8ugwikXIHKGOHBVEauDmO7yx579GW9GdgZMgsvNawS1.8XXpys4zbpaG3N0hvBLDNqQ8cyj
} 3, 29 --
} 1TEsA7jPiu6pxVkQ0AaY.w_9g8PB9XnyXBH.mz3Jp5TR9rQoxU7sHPwPjy_JqJd3m83fyNFhvPuQ
} 3, 29 --
} m3dVQ1abp.DifVvCA3Yb4FRYqIDZCOPX0xg8XKxpyzpYnmBp69hrSrCfj_6Snc8sD6UU9NOxJ93f
} 3, 29 --
} dJeWO1GznuG5eY4hKCAH0vmlMa8agqeM.M4tSZaIXP09yXCgaEMSAj.aDIpN4kUvtoJYN9MczS3D
} 3, 29 --
} GJoW6ohYzYMk05lp.BH7g8D8g_6wqQxWiS08xJAMwxRaDjlVrDb41LV9KtUsHqodh4TQT3Z2DqFT
} 3, 29 -- XNnKGXA-- 3, 29 -- Received: by 66.196.80.114; Thu, 18 Feb
} 2016 10:08:03 +0000 3, 29 -- Date: Thu, 18 Feb 2016 10:08:02 +0000
} (UTC) 3, 29 -- From: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 --
} Reply-To: Stephane Nizet {nizets2-at-yahoo.com} 3, 29 -- To:
} {microscopy-at-microscopy.com} 3, 29 -- Message-ID:
} {198161144.6166998.1455790082942.JavaMail.yahoo-at-mail.yahoo.com} 3, 29
} -- Subject: bias and gain correction on TEM cameras 3, 29 --
} MIME-Version: 1.0 3, 29 -- Content-Type: text/plain; charset=UTF-8 3,
} 29 -- Content-Transfer-Encoding: 7bit 3, 29 -- References:
} {198161144.6166998.1455790082942.JavaMail.yahoo.ref-at-mail.yahoo.com}
} ==============================End of -
} Headers==============================


Upozorneni:
Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.

Disclaimer:
If not expressly stated otherwise, this e-mail message (including any attached files) is intended purely for informational purposes and does not represent a binding agreement on the part of Institutes of AS CR. The text of this message and its attachments cannot be considered as a proposal to conclude a contract, neither the acceptance of a proposal to conclude a contract, nor any other legal act leading to concluding any contract; nor it does not create any pre-contractual liability on the part of Institutes of AS CR.



==============================Original Headers==============================
12, 48 -- From benada-at-biomed.cas.cz Thu Feb 18 05:39:25 2016
12, 48 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11])
12, 48 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IBdOm4001310
12, 48 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 05:39:25 -0600
12, 48 -- X-ASG-Debug-ID: 1455795548-05011e54257f2db0001-4CH8be
12, 48 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id tXxpXOM8wjJ82mks (version=TLSv1 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO); Thu, 18 Feb 2016 12:39:08 +0100 (CET)
12, 48 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz
12, 48 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32]
12, 48 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32
12, 48 -- Received: from u117ob02 (c111jn.mbu.cas.cz [147.231.44.12])
12, 48 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
12, 48 -- (No client certificate requested)
12, 48 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id DB7002C80E8;
12, 48 -- Thu, 18 Feb 2016 12:39:04 +0100 (CET)
12, 48 -- Date: Thu, 18 Feb 2016 12:39:04 +0100
12, 48 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
12, 48 -- To: nizets2-at-yahoo.com
12, 48 -- Cc: {microscopy-at-microscopy.com}
12, 48 -- Subject: Re: [Microscopy] bias and gain correction on TEM cameras
12, 48 -- Message-ID: {20160218123904.70cccbb3-at-u117ob02}
12, 48 -- X-ASG-Orig-Subj: Re: [Microscopy] bias and gain correction on TEM cameras
12, 48 -- In-Reply-To: {201602181019.u1IAJrKi025081-at-ns.microscopy.com}
12, 48 -- References: {201602181019.u1IAJrKi025081-at-ns.microscopy.com}
12, 48 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV
12, 48 -- =?UTF-8?B?xIxS?=
12, 48 -- X-Mailer: Claws Mail 3.11.1 (GTK+ 2.24.25; i586-pc-linux-gnu)
12, 48 -- MIME-Version: 1.0
12, 48 -- Content-Type: text/plain; charset=UTF-8
12, 48 -- X-IoP-CAS-MailScanner-ID: DB7002C80E8.86B0F
12, 48 -- X-IoP-CAS-MailScanner: Processed
12, 48 -- X-Spam-Status: No
12, 48 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32]
12, 48 -- X-Barracuda-Start-Time: 1455795548
12, 48 -- X-Barracuda-Encrypted: DHE-RSA-AES256-SHA
12, 48 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi
12, 48 -- X-Barracuda-Scan-Msg-Size: 6193
12, 48 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz
12, 48 -- X-Barracuda-BRTS-Status: 1
12, 48 -- X-Barracuda-Spam-Score: 0.50
12, 48 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=1000.0 KILL_LEVEL=9.0 tests=BSF_RULE7568M, BSF_SC0_MISMATCH_TO
12, 48 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.27131
12, 48 -- Rule breakdown below
12, 48 -- pts rule name description
12, 48 -- ---- ---------------------- --------------------------------------------------
12, 48 -- 0.00 BSF_SC0_MISMATCH_TO Envelope rcpt doesn't match header
12, 48 -- 0.50 BSF_RULE7568M Custom Rule 7568M
12, 48 -- Content-Transfer-Encoding: 8bit
12, 48 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IBdOm4001310
==============================End of - Headers==============================




From: WHITTAKS-at-si.edu
Date: Thu, 18 Feb 2016 13:47:18 -0600
Subject: [Microscopy] SEM- spring cleaning- filaments and Amray wehnelt up for grabs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If anyone is interested I have a spare Wehnelt that was part of an Amray 1810 unit as well as spare tungsten and LaB6 filaments that fit it.

The filaments also fit a Philips XL30 ESEM if an unmodified gun from prior to 2003.

Free but for the cost of shipping....


Scott Whittaker
Manager SEM Lab
Smithsonian Institution
National Museum of Natural History
PO Box 37012** MRC104
Washington DC 20013-7012
202-633-0891



==============================Original Headers==============================
7, 26 -- From WHITTAKS-at-si.edu Thu Feb 18 13:47:18 2016
7, 26 -- Received: from si-mailout04.si.edu (si-mailout04.si.edu [160.111.103.178])
7, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1IJlIs9015210
7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 13:47:18 -0600
7, 26 -- Received: from SI-MSECHT03.US.SINET.SI.EDU (si-msecht03.us.sinet.si.edu [160.111.49.155])
7, 26 -- by si-mailout04.si.edu (Postfix) with ESMTP id 2AB84E611D
7, 26 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 14:47:04 -0500 (EST)
7, 26 -- Received: from SI-MSEDAG04.US.SINET.SI.EDU ([169.254.4.210]) by
7, 26 -- SI-MSECHT03.US.SINET.SI.EDU ([160.111.49.149]) with mapi id 14.03.0266.001;
7, 26 -- Thu, 18 Feb 2016 14:47:03 -0500
7, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
7, 26 -- To: "'microscopy-at-microscopy.com'" {microscopy-at-microscopy.com}
7, 26 -- Subject: SEM- spring cleaning- filaments and Amray wehnelt up for grabs
7, 26 -- Thread-Topic: SEM- spring cleaning- filaments and Amray wehnelt up for grabs
7, 26 -- Thread-Index: AdFqhRXr8UgvAE1XQCC2/WxgPpM7Sw==
7, 26 -- Date: Thu, 18 Feb 2016 19:47:02 +0000
7, 26 -- Message-ID: {5D4F637E5C599649A11693E3B6EAC6293744A37C-at-si-msedag04.US.SINET.SI.EDU}
7, 26 -- Accept-Language: en-US
7, 26 -- Content-Language: en-US
7, 26 -- X-MS-Has-Attach:
7, 26 -- X-MS-TNEF-Correlator:
7, 26 -- x-originating-ip: [160.111.49.170]
7, 26 -- Content-Type: text/plain; charset="iso-8859-1"
7, 26 -- MIME-Version: 1.0
7, 26 -- Content-Transfer-Encoding: 8bit
7, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1IJlIs9015210
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Feb 2016 18:34:58 -0600
Subject: [Microscopy] viaWWW:How batch convert emi to other format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: 12hy1-at-queensu.ca

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: 12hy1-at-queensu.ca
Name: Hongbing Yu

Organization: Queen's University at Kingston

Title-Subject: [Filtered] How batch convert emi to other format

Message: Dear all,
We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I
can open them in TIA software, and can only export them one by one. That is quite annoying. Is there
anybody who has any idea how to batch convert the images?

Thanks
Hongbing Yu


Login Host: 130.15.34.113
Listserver Email Form V - 20120416
---------------------------------------------------------------------------




--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
15, 53 -- From microscopy.listserver-at-gmail.com Thu Feb 18 18:34:57 2016
15, 53 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171])
15, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J0YvHa018804
15, 53 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 18:34:57 -0600
15, 53 -- Received: by mail-ig0-f171.google.com with SMTP id y8so27224061igp.0
15, 53 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 16:34:43 -0800 (PST)
15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 53 -- d=gmail.com; s=20120113;
15, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
15, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
15, 53 -- bh=ct89n10F4wDUiH7r8OUh5Gq2bjWnOu8bZJvmjqTLs8A=;
15, 53 -- b=qwdaDEOQvhIiGg6FY6x6Wled575AHnbhAK1AbytHDi3D1nBx49vcl1PdiupJCcNFdS
15, 53 -- 2IQOKV9h/N0KFX3Y5Hkk8BO3W4nsklAbfUE+969vLUY1IcWQDg45pzlGYvzutBeq1yzt
15, 53 -- rceASx7ssFLpLM2JpKXX4mVt3esaU/d2EynPrXocaevYLPeaEJEBVRKSpp7rfiHWFMkq
15, 53 -- Qpo3favAwzzKx9J+5wamyc2QA+pGTxoRz6as5L8leun046b0YcvduHeIjMOFOxhR+e4+
15, 53 -- LDPMyordnyzT01MJosU+XnCJhRaArURchoT9Xa4XZ2PfNu/xl7kCK0FnEx/y0lqQVOwJ
15, 53 -- 3VoQ==
15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
15, 53 -- d=1e100.net; s=20130820;
15, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
15, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
15, 53 -- :content-transfer-encoding;
15, 53 -- bh=ct89n10F4wDUiH7r8OUh5Gq2bjWnOu8bZJvmjqTLs8A=;
15, 53 -- b=NKfXCWBz0O82b4vEcaHUWvpKp15GyBJJ66pCMsruNDCsSYy3+t8u8xrTGsZhk4sXIo
15, 53 -- szQjWdgszMpVQcks4lw6DnU68sep+aHa6bQOUyn1OsrYYA52wQIz8c0SCMt6wJgDHRsZ
15, 53 -- 2APyFGOEcAqIPUjUehfAcAhYpm+E5UJN7xDGgfiGskOLHkRiuTJ1MLf300tQJdKIdem7
15, 53 -- ONzD8mU1TIj6uozoyf+ns0S8jZAZZT0oFZskzt8Z93G4vuIvK6VZ+wSczIjYl2BdO0uc
15, 53 -- qd6QrEfKA5Eycy3kkE37KsZaiachUZwcOdzBO5Y+WcQQbNHdWXNd2pvnynIVC9y2SFK4
15, 53 -- 75KA==
15, 53 -- X-Gm-Message-State: AG10YOSLlvlyp406mE3kWPxN6TJe2TsZtzwWZFUUYMt/Uo7TiQ/3+Fb4eT6QulQ16/SfMg==
15, 53 -- X-Received: by 10.50.138.72 with SMTP id qo8mr6270594igb.81.1455842083450;
15, 53 -- Thu, 18 Feb 2016 16:34:43 -0800 (PST)
15, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
15, 53 -- by smtp.googlemail.com with ESMTPSA id k6sm2592199igd.8.2016.02.18.16.34.42
15, 53 -- for {microscopy-at-microscopy.com}
15, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
15, 53 -- Thu, 18 Feb 2016 16:34:42 -0800 (PST)
15, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
15, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
15, 53 -- {microscopylistserver-noreply-at-microscopy.com}
15, 53 -- Subject: viaWWW:How batch convert emi to other format
15, 53 -- References: {201602181540.u1IFeXWV002002-at-ns.microscopy.com}
15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
15, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
15, 53 -- X-Forwarded-Message-Id: {201602181540.u1IFeXWV002002-at-ns.microscopy.com}
15, 53 -- Message-ID: {56C66321.5000002-at-microscopy.com}
15, 53 -- Date: Thu, 18 Feb 2016 18:34:41 -0600
15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
15, 53 -- Gecko/20100101 Thunderbird/38.6.0
15, 53 -- MIME-Version: 1.0
15, 53 -- In-Reply-To: {201602181540.u1IFeXWV002002-at-ns.microscopy.com}
15, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
15, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Feb 2016 18:35:55 -0600
Subject: [Microscopy] viaWWW:Attn: LaB6 cathode/e-gun mfrs and vendors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: mlibbee-at-lbl.gov

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mlibbee-at-lbl.gov as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: mlibbee-at-lbl.gov
Name: Marissa Libbee

Organization: LBL

Title-Subject: [Filtered] Attn: LaB6 cathode/e-gun mfrs and vendors

Message: I'm interested in tracking down mfrs and vendors of anything LaB6 related. Please contact
me offline with company names and preferably, a contact name within the company. Thanks!

Login Host: 131.243.3.252
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
10, 52 -- From microscopy.listserver-at-gmail.com Thu Feb 18 18:35:55 2016
10, 52 -- Received: from mail-ig0-f174.google.com (mail-ig0-f174.google.com [209.85.213.174])
10, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J0Zt4Z019527
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 18:35:55 -0600
10, 52 -- Received: by mail-ig0-f174.google.com with SMTP id y8so25235427igp.1
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 16:35:41 -0800 (PST)
10, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=gmail.com; s=20120113;
10, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
10, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=eEhR22sct+4uMF6IiLwPRjdCzRdcdK6B41fyzCUFKyM=;
10, 52 -- b=lBYrC8DuD3PeVd/D14U1kCDUVg/9BxNYmR3zvT/G8HJv6MGsMo37/2kzKqJIW8qDRk
10, 52 -- ly20IH+o3tlEC+ixp2sGJVMwSBk7EhU/Qgt2valpDbgAMCfJsciT41o9OuFugq63LxPl
10, 52 -- yjNOtIRuYDZQGqBlUMH+2KXRvpBBSsovjZiEO37A+IBGHCWJdqeScysYSyp9SOYce2ue
10, 52 -- 8+Nl4PZVHtBjqF7kmJetQ5aKvdY1YKA+Wt4Wc7F/N+JYWeesdmEVMgJn2/0DRtwZQDXq
10, 52 -- UMvMb3/QqhQCo+jkDRzsq7++l/QiNSfMBPG1T32F25fdGmoufeYKQ6av6eag5r/cOdcn
10, 52 -- Al6A==
10, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=1e100.net; s=20130820;
10, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
10, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=eEhR22sct+4uMF6IiLwPRjdCzRdcdK6B41fyzCUFKyM=;
10, 52 -- b=BkRBbAQVmvIhKE9UT1FvOGhN0urYcnYxNaOV5dC0QWk9uXdnQomsU0Kek/Ari4uwpF
10, 52 -- GhZHi7LE2tfIfEoRviMQFteO3UP2YCr+rOVdNL5RXOpNzuNsqQqLXAb0UlYCrN2h4m1e
10, 52 -- 9udtJUHJqYlGXEDhBw35vzJTDjT31wZ73O4cCpquE6fGwmrir8yY0QrsrFpIdyCsf+a5
10, 52 -- 07AxnwOG86XqQuok9h4xYv+Bk+Z2rx1oe4rNXA4u6qKsc9MZXJFlUpGsx57mkulc6SZ0
10, 52 -- 7X/Pf9LT9QJKtshYKfx+8lwgbjkiX8ftAYI/dBiyBg/W7slsAQ0RZ4D62qv4+PFUcp/A
10, 52 -- hW5g==
10, 52 -- X-Gm-Message-State: AG10YOQfZbNXOLFKXs3RVQ14fYT34pW5dttbvTi3RZy/TqOiO35zLm3sk5gAP/WephUpgA==
10, 52 -- X-Received: by 10.50.78.130 with SMTP id b2mr892102igx.71.1455842141691;
10, 52 -- Thu, 18 Feb 2016 16:35:41 -0800 (PST)
10, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
10, 52 -- by smtp.googlemail.com with ESMTPSA id j136sm4711973ioj.32.2016.02.18.16.35.41
10, 52 -- for {microscopy-at-microscopy.com}
10, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
10, 52 -- Thu, 18 Feb 2016 16:35:41 -0800 (PST)
10, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
10, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
10, 52 -- {microscopylistserver-noreply-at-microscopy.com}
10, 52 -- Subject: viaWWW:Attn: LaB6 cathode/e-gun mfrs and vendors
10, 52 -- References: {201602181853.u1IIrk8J011042-at-ns.microscopy.com}
10, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
10, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
10, 52 -- X-Forwarded-Message-Id: {201602181853.u1IIrk8J011042-at-ns.microscopy.com}
10, 52 -- Message-ID: {56C6635C.1060802-at-microscopy.com}
10, 52 -- Date: Thu, 18 Feb 2016 18:35:40 -0600
10, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
10, 52 -- Gecko/20100101 Thunderbird/38.6.0
10, 52 -- MIME-Version: 1.0
10, 52 -- In-Reply-To: {201602181853.u1IIrk8J011042-at-ns.microscopy.com}
10, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Feb 2016 18:37:18 -0600
Subject: [Microscopy] viaWWW:EM Connectome Annotation Team Manager (362-908 )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: murphyv-at-janelia.hhmi.org

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both murphyv-at-janelia.hhmi.org as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: murphyv-at-janelia.hhmi.org
Name: Veronica Murphy

Organization: HHMI Janelia Research Campus

Title-Subject: [Filtered] EM Connectome Annotation Team Manager (362-908 )

Message: The Janelia Research Campus of the Howard Hughes Medical Institute (Janelia) is using
high-throughput electron microscopy (EM) to completely image multiple brains and nervous systems of
the fruit fly, Drosophila melanogaster. Janelia scientists will utilize these massive data sets to
reconstruct neuronal circuits, ultimately culminating in the complete connectome-oor wiring
diagram-oof the fly brain. The connectome will provide the most complete anatomical description of a
complex nervous system to date in an organism uniquely suited to combine this anatomical information
with functional imaging, electrophysiology, quantitative behavioral assays, precise genetic
manipulation of identified cell types and computational modeling. Thus we expect these circuit-level
reconstructions to inform specific biological experiments, enabling dramatic leaps in our
understanding of brain function.

Janelia is seeking a talented individual to assemble, train, and lead a group of approximately ten
neuron tracing staff, with potential for a significantly larger effort based on the established
success of the initial team. In order to be successful, the manager must have research experience in
neuroanatomy, preferably in the application of electron microscopy to elucidate neurobiological
function. Knowledge of Drosophila biology and/or software for reconstructing objects from serial
section images is strongly desired. The manager will collaborate with scientists (at Janelia and
elsewhere) whose research is targeted toward understanding of specific circuits and functions in the
fly brain, guiding their team of highly trained specialists to identify, trace, and annotate
specific collections of neurons from the vast data being produced. The manager will also collaborate
closely with the project teams producing the EM data and developing the algorithms and tools that
enable the work of their team. Thus, the ideal candidate for this position must have a neuroscience
background, the ability to teach scientific concepts, and experience in supervising and leading the
work of others in a dynamic setting. The candidate must further possess an innate sense of pace and
urgency, and excel at working in a team science environment to achieve challenging goals,
prioritizing multiple assignments, and communicating clearly to a broad group of collaborators.
Experience in supervising and leading the work of others is desired but not strictly required.

HHMI is an Equal Opportunity Employer.

Please apply online at
https://hhmi-openhire.silkroad.com/epostings/index.cfm?fuseaction=app.jobinfo&jobid=362&company_id=16908&version=1&source=ONLINE&jobOwner=992274&aid=1.


Login Host: 206.241.0.254
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
16, 53 -- From microscopy.listserver-at-gmail.com Thu Feb 18 18:37:18 2016
16, 53 -- Received: from mail-ig0-f172.google.com (mail-ig0-f172.google.com [209.85.213.172])
16, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J0bIEx020829
16, 53 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 18:37:18 -0600
16, 53 -- Received: by mail-ig0-f172.google.com with SMTP id y8so27258394igp.0
16, 53 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 16:37:04 -0800 (PST)
16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 53 -- d=gmail.com; s=20120113;
16, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
16, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
16, 53 -- bh=JyiaWmx3OOXnFimGlyvNlGSLGK+4S3Jz1e42JeycPGk=;
16, 53 -- b=OYz4y32YdlWkED0nxJnUJWmCVJMQA+WvnkD1evuABAjUTGL969RZzBW8CvJjXdOqI7
16, 53 -- QPac4Xp/TgPD3RWySZ3xrB2GibTPExomsFnfOTUsuh8hhmYuzUrKbXTMBKfvJun3baIs
16, 53 -- n/ZkQ/TaLVqAXaGYCn8ZOGO+JOZYGj5hayzQAfP9PwaeHR9qQjTYYrLMMDMDMsYy2bf6
16, 53 -- FyPEUouKfxeUghAXa0Vt3csxKEK/vqPIe5br0g9fShSPvmctTTERZ09+x/q3kOxWIwAw
16, 53 -- SqDvsZQxvV5FuT22VyBzVuaFI+NmKXHHu/hxf6uUAecPCJz3i4Yv/YS+2sb8cd6DteQE
16, 53 -- FkcA==
16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 53 -- d=1e100.net; s=20130820;
16, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
16, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
16, 53 -- :content-transfer-encoding;
16, 53 -- bh=JyiaWmx3OOXnFimGlyvNlGSLGK+4S3Jz1e42JeycPGk=;
16, 53 -- b=jppBODwntGOo6uVoWnimyMFiEkcmWJR6nRJ7zHIH7F6P8xNn8E0qwiyMm+XV8RMko/
16, 53 -- aXxf+rzfqXOqabYKD+IYh+QcePh3QmDLlAzZJ36MTiRMRHLQeOuf3NQUVz+OmNHyg8YJ
16, 53 -- r/NrvjW6vZciD2lN6A/qcQVhpxymH95KXg/RZWEdqJ85A7cPrTF/ywCHwI6WePBLjCDx
16, 53 -- 8gMxSn1mLG5CZd8MmlQeeN0xmDqVG88EZ1oqzedF5wEkppEjv07Q9YAVgS7zaf7DcrvB
16, 53 -- COoYSpFttUL7dIHcm1q4QhJrq+wA5Rjn0Eba81QP6TbIMcxfel2L3pCjQaV8K3yn+zt8
16, 53 -- 8KQw==
16, 53 -- X-Gm-Message-State: AG10YOTWpQAk5QyLr9M0rGDkhJG15DNQ8NGKLcYQT2vbnLgOvCidVjiyeHZvec6AqL4hPA==
16, 53 -- X-Received: by 10.50.138.72 with SMTP id qo8mr6279211igb.81.1455842224075;
16, 53 -- Thu, 18 Feb 2016 16:37:04 -0800 (PST)
16, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
16, 53 -- by smtp.googlemail.com with ESMTPSA id l6sm2590279igv.10.2016.02.18.16.37.03
16, 53 -- for {microscopy-at-microscopy.com}
16, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 53 -- Thu, 18 Feb 2016 16:37:03 -0800 (PST)
16, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
16, 53 -- {microscopylistserver-noreply-at-microscopy.com}
16, 53 -- Subject: viaWWW:EM Connectome Annotation Team Manager (362-908 )
16, 53 -- References: {201602182037.u1IKbiXm004793-at-ns.microscopy.com}
16, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
16, 53 -- X-Forwarded-Message-Id: {201602182037.u1IKbiXm004793-at-ns.microscopy.com}
16, 53 -- Message-ID: {56C663AF.70300-at-microscopy.com}
16, 53 -- Date: Thu, 18 Feb 2016 18:37:03 -0600
16, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
16, 53 -- Gecko/20100101 Thunderbird/38.6.0
16, 53 -- MIME-Version: 1.0
16, 53 -- In-Reply-To: {201602182037.u1IKbiXm004793-at-ns.microscopy.com}
16, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed
16, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: wim.hagen-at-me.com
Date: Thu, 18 Feb 2016 20:53:36 -0600
Subject: [Microscopy] Re: viaWWW:How batch convert emi to other format

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Hongbing,

TIA folder export:
- Open menu Tools\Components.
- Click ,AeuInstall,Aeu.
- Type ,Aeufolderexport.wsc,Aeu.
- ,Aeuok".
Now that this component is installed, there will be an additional menu in the processing tasks.
Check settings and hit Export.

Best,

Wim Hagen
EMBL Heidelberg

} On Feb 19, 2016, at 1:58 AM, microscopy.listserver-at-gmail.com wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} X-from: 12hy1-at-queensu.ca
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at http://www.microscopy.com/MLFormMail.html
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying
} please copy both 12hy1-at-queensu.ca as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: 12hy1-at-queensu.ca
} Name: Hongbing Yu
}
} Organization: Queen's University at Kingston
}
} Title-Subject: [Filtered] How batch convert emi to other format
}
} Message: Dear all,
} We have an FEI TEM microscope. The STEM images Acquired in TIA software are saved in emi format. I
} can open them in TIA software, and can only export them one by one. That is quite annoying. Is there
} anybody who has any idea how to batch convert the images?
}
} Thanks
} Hongbing Yu
}
}
} Login Host: 130.15.34.113
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
}
}
} --
} ===========================================
} Do not reply to this message it is from
} the Microscopy Listserver NO-REPLY forwarding
} system. You should send a new message to
}
} Microscopy-at-Microscopy.com
}
} ============================================
}
} ==============================Original Headers==============================
} 15, 53 -- From microscopy.listserver-at-gmail.com Thu Feb 18 18:34:57 2016
} 15, 53 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171])
} 15, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J0YvHa018804
} 15, 53 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 18:34:57 -0600
} 15, 53 -- Received: by mail-ig0-f171.google.com with SMTP id y8so27224061igp.0
} 15, 53 -- for {microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 16:34:43 -0800 (PST)
} 15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 15, 53 -- d=gmail.com; s=20120113;
} 15, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
} 15, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
} 15, 53 -- bh=ct89n10F4wDUiH7r8OUh5Gq2bjWnOu8bZJvmjqTLs8A=;
} 15, 53 -- b=qwdaDEOQvhIiGg6FY6x6Wled575AHnbhAK1AbytHDi3D1nBx49vcl1PdiupJCcNFdS
} 15, 53 -- 2IQOKV9h/N0KFX3Y5Hkk8BO3W4nsklAbfUE+969vLUY1IcWQDg45pzlGYvzutBeq1yzt
} 15, 53 -- rceASx7ssFLpLM2JpKXX4mVt3esaU/d2EynPrXocaevYLPeaEJEBVRKSpp7rfiHWFMkq
} 15, 53 -- Qpo3favAwzzKx9J+5wamyc2QA+pGTxoRz6as5L8leun046b0YcvduHeIjMOFOxhR+e4+
} 15, 53 -- LDPMyordnyzT01MJosU+XnCJhRaArURchoT9Xa4XZ2PfNu/xl7kCK0FnEx/y0lqQVOwJ
} 15, 53 -- 3VoQ==
} 15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
} 15, 53 -- d=1e100.net; s=20130820;
} 15, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
} 15, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
} 15, 53 -- :content-transfer-encoding;
} 15, 53 -- bh=ct89n10F4wDUiH7r8OUh5Gq2bjWnOu8bZJvmjqTLs8A=;
} 15, 53 -- b=NKfXCWBz0O82b4vEcaHUWvpKp15GyBJJ66pCMsruNDCsSYy3+t8u8xrTGsZhk4sXIo
} 15, 53 -- szQjWdgszMpVQcks4lw6DnU68sep+aHa6bQOUyn1OsrYYA52wQIz8c0SCMt6wJgDHRsZ
} 15, 53 -- 2APyFGOEcAqIPUjUehfAcAhYpm+E5UJN7xDGgfiGskOLHkRiuTJ1MLf300tQJdKIdem7
} 15, 53 -- ONzD8mU1TIj6uozoyf+ns0S8jZAZZT0oFZskzt8Z93G4vuIvK6VZ+wSczIjYl2BdO0uc
} 15, 53 -- qd6QrEfKA5Eycy3kkE37KsZaiachUZwcOdzBO5Y+WcQQbNHdWXNd2pvnynIVC9y2SFK4
} 15, 53 -- 75KA==
} 15, 53 -- X-Gm-Message-State: AG10YOSLlvlyp406mE3kWPxN6TJe2TsZtzwWZFUUYMt/Uo7TiQ/3+Fb4eT6QulQ16/SfMg==
} 15, 53 -- X-Received: by 10.50.138.72 with SMTP id qo8mr6270594igb.81.1455842083450;
} 15, 53 -- Thu, 18 Feb 2016 16:34:43 -0800 (PST)
} 15, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
} 15, 53 -- by smtp.googlemail.com with ESMTPSA id k6sm2592199igd.8.2016.02.18.16.34.42
} 15, 53 -- for {microscopy-at-microscopy.com}
} 15, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
} 15, 53 -- Thu, 18 Feb 2016 16:34:42 -0800 (PST)
} 15, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
} 15, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
} 15, 53 -- {microscopylistserver-noreply-at-microscopy.com}
} 15, 53 -- Subject: viaWWW:How batch convert emi to other format
} 15, 53 -- References: {201602181540.u1IFeXWV002002-at-ns.microscopy.com}
} 15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
} 15, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
} 15, 53 -- X-Forwarded-Message-Id: {201602181540.u1IFeXWV002002-at-ns.microscopy.com}
} 15, 53 -- Message-ID: {56C66321.5000002-at-microscopy.com}
} 15, 53 -- Date: Thu, 18 Feb 2016 18:34:41 -0600
} 15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
} 15, 53 -- Gecko/20100101 Thunderbird/38.6.0
} 15, 53 -- MIME-Version: 1.0
} 15, 53 -- In-Reply-To: {201602181540.u1IFeXWV002002-at-ns.microscopy.com}
} 15, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
} 15, 53 -- Content-Transfer-Encoding: 7bit
} ==============================End of - Headers==============================



==============================Original Headers==============================
7, 27 -- From wim.hagen-at-me.com Thu Feb 18 20:53:36 2016
7, 27 -- Received: from pv33p04im-asmtp001.me.com (pv33p04im-asmtp001.me.com [17.143.181.10])
7, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1J2raff023321
7, 27 -- for {Microscopy-at-microscopy.com} ; Thu, 18 Feb 2016 20:53:36 -0600
7, 27 -- Received: from wim-hagens-macbook-pro.local.pool.embl.de
7, 27 -- (unknown [194.94.44.220]) by pv33p04im-asmtp001.me.com
7, 27 -- (Oracle Communications Messaging Server 7.0.5.36.0 64bit (built Sep 8 2015))
7, 27 -- with ESMTPSA id {0O2R0085PXCMH640-at-pv33p04im-asmtp001.me.com} for
7, 27 -- Microscopy-at-microscopy.com; Fri, 19 Feb 2016 02:53:14 +0000 (GMT)
7, 27 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10432:,,
7, 27 -- definitions=2016-02-19_02:,, signatures=0
7, 27 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 spamscore=0
7, 27 -- clxscore=1011 suspectscore=2 malwarescore=0 phishscore=0 adultscore=0
7, 27 -- bulkscore=0 classifier=spam adjust=0 reason=mlx scancount=1
7, 27 -- engine=8.0.1-1510270003 definitions=main-1602190039
7, 27 -- Content-type: text/plain; charset=utf-8
7, 27 -- MIME-version: 1.0 (Mac OS X Mail 8.2 \(2104\))
7, 27 -- Subject: Re: [Microscopy] viaWWW:How batch convert emi to other format
7, 27 -- From: Wim Hagen {wim.hagen-at-me.com}
7, 27 -- In-reply-to: {201602190058.u1J0wuXt027832-at-ns.microscopy.com}
7, 27 -- Date: Fri, 19 Feb 2016 03:53:09 +0100
7, 27 -- Message-id: {EA4C4200-6FBC-4DD6-9DC2-B4F92F0BF494-at-me.com}
7, 27 -- References: {201602190058.u1J0wuXt027832-at-ns.microscopy.com}
7, 27 -- To: 12hy1-at-queensu.ca, Microscopy-at-microscopy.com
7, 27 -- X-Mailer: Apple Mail (2.2104)
7, 27 -- Content-Transfer-Encoding: 8bit
7, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1J2raff023321
==============================End of - Headers==============================




From: john.mitchels-at-gmail.com
Date: Fri, 19 Feb 2016 08:15:12 -0600
Subject: [Microscopy] Consensus on UA replacement stains

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers

I am need of some experience here. Has anyone played with UA
replacements such samarium and gadolinium triacetate for en bloc
staining? How does this compare in performance, permeation,
precipitation etc? Is it an acceptable alternative for all tissues or
just selected ones. I am aware of many papers on the mater, and my own
dabbling but I tend to find the microscopy list opinion a little
easier to swallow.

Thanks in advance
J

==============================Original Headers==============================
3, 38 -- From john.mitchels-at-gmail.com Fri Feb 19 08:15:11 2016
3, 38 -- Received: from mail-wm0-f42.google.com (mail-wm0-f42.google.com [74.125.82.42])
3, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1JEFBuD011339
3, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Feb 2016 08:15:11 -0600
3, 38 -- Received: by mail-wm0-f42.google.com with SMTP id c200so77720896wme.0
3, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Feb 2016 06:14:57 -0800 (PST)
3, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 38 -- d=gmail.com; s=20120113;
3, 38 -- h=mime-version:date:message-id:subject:from:to:content-type;
3, 38 -- bh=if/Hc/CRlGCJBcvwhLuAHFO1tSr8HehtP22miQ3jpdI=;
3, 38 -- b=v+nm99XGRCdTjzGilgLwNm0eURMbG8SMjWxa0TJ6GP8FrptLTx17FYnbv/IaC6oHGq
3, 38 -- anADuT2+8ar7PuNnYt0ZIWfOXAYOh11Qug3S66IjA3+JT9zmzCnid56s/KksJDnqpIM8
3, 38 -- nQA6NxmI3YNrjiLRpDJzGJPbgffNvbkUgXLedbfjDyPBMQkqDxr5sa2eFg7jWa3ed4im
3, 38 -- 9OlOulirvToRK28Ni5Lw9R3CqTgvlVFp0YKzNQoVnMLwMtgmbMAQED6qbmO466zX88Je
3, 38 -- 4H5tGzgp+SWW+8WqGu+Jc+ZCHX9zL+YlWf/8LZppDQT1n/qMFYtr3GY29yYRWVstqNvx
3, 38 -- rgbg==
3, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
3, 38 -- d=1e100.net; s=20130820;
3, 38 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to
3, 38 -- :content-type;
3, 38 -- bh=if/Hc/CRlGCJBcvwhLuAHFO1tSr8HehtP22miQ3jpdI=;
3, 38 -- b=ZCTZ6VB+/JikTzstnky23EwrUvH2q/jZrpFai0NLZ8hnaHgpCquuHgToqKr/La7Yz+
3, 38 -- j3J91xkGl9khEeSXRD3iLz5IOE7idvkWo/Q0ssHZ2+UBTlwwWenFBs5sh6SJMQ2pmS6g
3, 38 -- kNlzY1pggafOyiDZrtUJwneAZdKBHCCFgSP4cwUioyoy17vJiKud3NsJHXCKFpvzlHv2
3, 38 -- 2dBDJfonVnh6dol21lLTwWrBzt6oN3eCiAF1umVV+jDdiXSS3M361sKmnyKvE1VyvkiD
3, 38 -- 0VFYcdenRoXl3XxomXeykusuuwizhgqOOmu3CDI4+FXMV12HQcd2tazpHCbaPULVFJoK
3, 38 -- Vfgw==
3, 38 -- X-Gm-Message-State: AG10YORkQpzOiWbX8QMChnNVaDY7yjRP/tgnHfaY6gadCDvGr94RWn2ZYs8JGTWyVX/Wc0I+zVhuRcheCDKycw==
3, 38 -- MIME-Version: 1.0
3, 38 -- X-Received: by 10.28.33.84 with SMTP id h81mr10029796wmh.36.1455891296921;
3, 38 -- Fri, 19 Feb 2016 06:14:56 -0800 (PST)
3, 38 -- Received: by 10.28.24.68 with HTTP; Fri, 19 Feb 2016 06:14:56 -0800 (PST)
3, 38 -- Date: Fri, 19 Feb 2016 15:14:56 +0100
3, 38 -- Message-ID: {CAHX7yTQaj-xSXeARoxRc+dYx_xP4Xpqa=cOCDa6UJ_EoShUdzg-at-mail.gmail.com}
3, 38 -- Subject: Consensus on UA replacement stains
3, 38 -- From: John Mitchels {john.mitchels-at-gmail.com}
3, 38 -- To: Microscopy-at-microscopy.com
3, 38 -- Content-Type: text/plain; charset=UTF-8
==============================End of - Headers==============================




From: hlowers-at-usgs.gov
Date: Fri, 19 Feb 2016 14:33:38 -0600
Subject: [Microscopy] EPMA 2016 Topical Conference and Student Support

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Announcing a MAS Topical Conference, Electron Probe Microanalysis 2016
May 16-19, 2016 at the University of Wisconsin-Madison


TOPICAL CONFERENCE PROGRAM
-Practical microanalysis for beginners to professionals
-Plenary Conference including user meetings, tutorials, group
discussion, problem solving, and instrument demos
-Quantitative analysis using the electron microprobe and SEM with EDS and WDS
-Improvements in microanalysis from measurement to accuracy
-Trace element analysis
-Compositional x-ray mapping and cathodoluminescence
-Applications in earth science, materials science, and industry

STUDENT FINANCIAL SUPPORT AVAILABLE
-Early career scholars: undergraduate, graduate, postdoc, and early
career professionals may apply
-Priority will be given to those with co-sponsorship from a
university, advisor, or employer
-ECS awardees may submit a 2-page abstract for platform or poster presentation.
-Awardees will receive a maximum of $750 reimbursement for travel,
lodging, and registration.


Please visit for more information
http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016


Heather Lowers, EPMA 2016 ECS Coordinator
Paul Carpenter, EPMA 2016 Planning Chair

==============================Original Headers==============================
8, 75 -- From hlowers-at-usgs.gov Fri Feb 19 14:33:36 2016
8, 75 -- Received: from smtp3.doi.gov (SMTP3.doi.gov [137.227.82.13])
8, 75 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1JKXXBD015921
8, 75 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Feb 2016 14:33:33 -0600
8, 75 -- Received: from smtp1.smgd.doi.gov (10.10.82.11) by smtp3.doi.gov (10.10.82.23)
8, 75 -- with Microsoft SMTP Server (TLS) id 14.2.347.0; Fri, 19 Feb 2016 13:33:17
8, 75 -- -0700
8, 75 -- DKIM-Signature: v=1; a=rsa-sha256; d=usgs.gov; s=mail; c=relaxed/simple;
8, 75 -- q=dns/txt; i=-at-usgs.gov; t=1455913998; x=1458505998;
8, 75 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc:MIME-Version:Content-Type:
8, 75 -- Content-Transfer-Encoding:Content-ID:Content-Description:Resent-Date:Resent-From:
8, 75 -- Resent-Sender:Resent-To:Resent-Cc:Resent-Message-ID:In-Reply-To:References:List-Id:
8, 75 -- List-Help:List-Unsubscribe:List-Subscribe:List-Post:List-Owner:List-Archive;
8, 75 -- bh=xoJr7gpmIQmvRPKGhcUJLeseHTsbH4aOy1FkMCVd9HU=;
8, 75 -- b=b4JgjJGetAWqnoH4VsIgiJcXYu80sJDzO4KnRclVmCoGpWpDzQffCzdCmDbdrRbH
8, 75 -- Cqc2/bAI2ZJyG49OYY22UGQcCZ1Qtwoe0VDrT/vS+G98kTehjALfump+5fqc01Zr
8, 75 -- iO82rWD6tyZtmLNPg7S1l1H4/YV+DwbfR7x9HF0J6wQ=;
8, 75 -- DKIM-Signature: v=1; a=rsa-sha256; d=usgs.gov; s=mail; c=relaxed/simple;
8, 75 -- q=dns/txt; i=-at-usgs.gov; t=1455913998; x=1458505998;
8, 75 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc:MIME-Version:Content-Type:
8, 75 -- Content-Transfer-Encoding:Content-ID:Content-Description:Resent-Date:Resent-From:
8, 75 -- Resent-Sender:Resent-To:Resent-Cc:Resent-Message-ID:In-Reply-To:References:List-Id:
8, 75 -- List-Help:List-Unsubscribe:List-Subscribe:List-Post:List-Owner:List-Archive;
8, 75 -- bh=xoJr7gpmIQmvRPKGhcUJLeseHTsbH4aOy1FkMCVd9HU=;
8, 75 -- b=b4JgjJGetAWqnoH4VsIgiJcXYu80sJDzO4KnRclVmCoGpWpDzQffCzdCmDbdrRbH
8, 75 -- Cqc2/bAI2ZJyG49OYY22UGQcCZ1Qtwoe0VDrT/vS+G98kTehjALfump+5fqc01Zr
8, 75 -- iO82rWD6tyZtmLNPg7S1l1H4/YV+DwbfR7x9HF0J6wQ=;
8, 75 -- X-AuditID: 0a0a520b-f79c86d000006da4-a5-56c77c0eb8d1
8, 75 -- Received: from gsmtp1.doi.gov ( [10.10.82.15]) (using TLS with cipher
8, 75 -- AES128-SHA (128/128 bits)) (Client did not present a certificate) by
8, 75 -- smtp1.smgd.doi.gov (Hello) with SMTP id 77.EE.28068.E0C77C65; Fri, 19 Feb
8, 75 -- 2016 13:33:18 -0700 (MST)
8, 75 -- Received: from mail-vk0-f47.google.com (209.85.213.47) by gsmtp1.doi.gov
8, 75 -- (137.227.82.15) with Microsoft SMTP Server (TLS) id 14.2.347.0; Fri, 19 Feb
8, 75 -- 2016 13:33:18 -0700
8, 75 -- Received: by mail-vk0-f47.google.com with SMTP id e185so84816076vkb.1
8, 75 -- for {Microscopy-at-microscopy.com} ; Fri, 19 Feb 2016 12:33:18 -0800 (PST)
8, 75 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
8, 75 -- d=1e100.net; s=20130820;
8, 75 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to
8, 75 -- :content-type;
8, 75 -- bh=xoJr7gpmIQmvRPKGhcUJLeseHTsbH4aOy1FkMCVd9HU=;
8, 75 -- b=RcuhJTr9Ca4H0o2XSvoMKAoYrF4h4jW3dW3sUK+LTbIm9Q+EKkZrkrAl3J2MbGgf0y
8, 75 -- 4VdXVDzjCp3CS8Jn5P0NbESpWEMTi7zF0KwnbKz4b57J0KmS9I5GJ9MwzivzwkWBB1ei
8, 75 -- 7y0aafzs0V+dxx4xAQjJspqkdXD5dYoDhrvPjEE6fgP4PBPXL2PZEFts0kCG2fULmc1F
8, 75 -- LyqsQ9dkc/Z6Ke4zq6IMS8xpzruQnw6bVAIdgmKvCYA1DFyfVM7xu2YEV8mftGr6Ksvn
8, 75 -- 07k4B1EbfmAH5zZYM7aNPkxUNAR9XOnkcmEUsIzu4QQxKgmEwJfYSf0AxCGVHWGXLs4T
8, 75 -- 6VqQ==
8, 75 -- X-Gm-Message-State: AG10YOREpfqbj29qRuMsJzLxlOS2LXy6/fosFtHfHSkElE9n8j6D+XEcpP7k2kmg3ftVw5snV+KMsaZiJZQpjDkVarvQ4h7Xdezk6n+/o2r14qhj1O/evNObnSxanGsdMVv38SqPy7JeCD2obXdLAGO6AYueCxZmwU56eIM8NGpVdsDek0VatGdFYFHLOhKyLMRrW3o8J/frEwXLHWxzn8rSvrZygSjiSXCXzPMKdSQ0
8, 75 -- X-Received: by 10.31.162.20 with SMTP id l20mr11298429vke.137.1455913992653;
8, 75 -- Fri, 19 Feb 2016 12:33:12 -0800 (PST)
8, 75 -- MIME-Version: 1.0
8, 75 -- X-Received: by 10.31.162.20 with SMTP id l20mr11298355vke.137.1455913991365;
8, 75 -- Fri, 19 Feb 2016 12:33:11 -0800 (PST)
8, 75 -- Received: by 10.31.228.129 with HTTP; Fri, 19 Feb 2016 12:33:11 -0800 (PST)
8, 75 -- Date: Fri, 19 Feb 2016 13:33:11 -0700
8, 75 -- Message-ID: {CAOx2-aAZvHVbJsUcekkWmOObX+isp_B5+uTGEMps+2ieSta_EQ-at-mail.gmail.com}
8, 75 -- Subject: EPMA 2016 Topical Conference and Student Support
8, 75 -- From: "Lowers, Heather" {hlowers-at-usgs.gov}
8, 75 -- To: microscopy {Microscopy-at-microscopy.com}
8, 75 -- Content-Type: text/plain; charset="UTF-8"
8, 75 -- X-Gm-Spam: 0
8, 75 -- X-Gm-Phishy: 0
8, 75 -- X-CFilter-Loop: Reflected
8, 75 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFnrCLMWRmVeSWpSXmKPExsXCxfTrgy5fzfEwg+svdCz+HG5gd2D0+Nj9
8, 75 -- mimAMYrLJiU1J7MstUjfLoEr49tcs4ITnBVvtl9kb2CcwNHFyMXBIrCFVeLFkovsXYycHBIC
8, 75 -- JhL7519hhrDFJC7cW88GUiQk8IVR4uXcZ+wQzhpGiakzLzJDOFMZJY50vmICcSQEZrJKLDy3
8, 75 -- mh3CaWCSmH7kFwvEsGqJr8vbWEFsXgFBiZMzn0DFSyTO32sHauAAGuUtseMFE4jJIqAq8fdx
8, 75 -- OkR1gMT0nd1gFcIClhK3L/KChNkEtCQmnz7GBmKLANltvfvAPmAW0JRo3f4b6hseiSlzYT7j
8, 75 -- k1hzZQvYUiEBWYm+CT1QB0hKHFxxg2UCo9gsJLfNQjJqASPTKkah4tySAkO94tz0FL2U/Ey9
8, 75 -- 9PyyTYygsOcK4t7BeOKmxSFGAQ5GJR7ehsTjYUKsiWXFlbmHGCU4mJVEeB8GAoV4UxIrq1KL
8, 75 -- 8uOLSnNSiw8xSnOwKInzOtglBQsJpCeWpGanphakFsFkmTg4pRoYs9oiFr6zttzPNt0nYfO1
8, 75 -- LDvNjZJMvpnnuTbmefrFmDnbPN925ui/DSdj9j742zd1t9OFWe4Nr/q+vNljvfO48+8FLtt8
8, 75 -- 7OablJcmxkq8M825cCk8tyWmXip4yU3V+aaOpgXSDE6NNU6HP13ba5OwaV2T17zgG5NrI9Sa
8, 75 -- r9iU+62yPW3S8/66EktxRqKhFnNRcSIA48GOdXcCAAA=
==============================End of - Headers==============================




From: dblackburn2000-at-yahoo.com
Date: Sat, 20 Feb 2016 10:55:20 -0600
Subject: [Microscopy] question - Allergy to histological solvents?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello

After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes.-* These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches.-* This has made staining and coverslipping very difficult, even with-* hood.-*-*-*Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount?-* thanks very much!

Daniel Blackburn
Biology, Trinity College (Hartford CT)
-*


==============================Original Headers==============================
4, 29 -- From dblackburn2000-at-yahoo.com Sat Feb 20 10:55:19 2016
4, 29 -- Received: from nm13-vm0.bullet.mail.bf1.yahoo.com (nm13-vm0.bullet.mail.bf1.yahoo.com [98.139.213.79])
4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1KGtJqN017594
4, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 20 Feb 2016 10:55:19 -0600
4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455987305; bh=GYAjj76GzomM7vop6UUnJyXkz0u+wZUkwa3SDd0g15A=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=TPxqPNC/tvQBHsTKLGuQ51Jxg7PrRk1Z77IjDYAtNrZPBfyLnjxprl57gqSAmcE6keQ080eKlTjoR/clH40sa3IPkQqVdl3HUZaugXs9TzpNOF+PJyPx7Ey2G68vm51CkrE0pfnoYku9ZaNs+8dasZxwEoJOt5N4tyekwNeyVH4GCpXRv4EFgmxNTtJtzt5Z6fJ9cGdjkoEooZibBz86jBWN70VTdga00AoibIBFXh/0g4qvxNfJKsxYB/XhmCgB/ZG4fK8She7vD2xu2k/W1HqNDBSK77t/7eLBoeuKid0158KLd6UXCr9j7zSErsJ0xwuMBw9LmRrmi9iC10fEHg==
4, 29 -- Received: from [66.196.81.170] by nm13.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000
4, 29 -- Received: from [98.139.212.222] by tm16.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000
4, 29 -- Received: from [127.0.0.1] by omp1031.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000
4, 29 -- X-Yahoo-Newman-Property: ymail-3
4, 29 -- X-Yahoo-Newman-Id: 532123.73121.bm-at-omp1031.mail.bf1.yahoo.com
4, 29 -- X-YMail-OSG: pQ6H50EVM1mOAEYBONpigG3IZYomqCLazwII_MkU0fjcMa3_mrHLGMVY9za3Cb5
4, 29 -- rtfU9TxN46HHIjZZmoXVODUSN2PFdj3kF9Ae_nSuAc71MZ2azIzwmB4U4FJaEynMUv_j1mX4wReD
4, 29 -- mREMmAAZnMyIZTZuaQekhHvng_BLwBMnJ5vL9WPtVQxYrY2f1mGvDO4yzQZ31iy_AKLJtY9aZ2hV
4, 29 -- CeSPnr1ZrqqTnIR1XTfmWUi4OObTKxkXdcRKYUc0J7cKTEjtonO5nAK5yoXFFcZrxWTjUHSaQl8q
4, 29 -- 5E2S3qBWMCAyAOkd8im.uNmteAlj7usrcZXcQvG4cRrGBi8zNdGCptRXt2.2knijRUVID_.fcHWl
4, 29 -- 4XVdFdmAJirUe0hNywHM6LYT87WdteIDhiNyWHloS5g3DIr8jFKjILiRsuMQqSAiR.ERWgXqlZvj
4, 29 -- rvi0eS7x7hsidXf0j7fHi6aGnkElT8D8cxLc0Ivs2n9rouVgLONbz
4, 29 -- Received: by 76.13.27.50; Sat, 20 Feb 2016 16:55:05 +0000
4, 29 -- Date: Sat, 20 Feb 2016 16:55:04 +0000 (UTC)
4, 29 -- From: daniel blackburn {dblackburn2000-at-yahoo.com}
4, 29 -- Reply-To: daniel blackburn {dblackburn2000-at-yahoo.com}
4, 29 -- To: {Microscopy-at-microscopy.com}
4, 29 -- Message-ID: {370209020.7409633.1455987304853.JavaMail.yahoo-at-mail.yahoo.com}
4, 29 -- Subject: question - Allergy to histological solvents?
4, 29 -- MIME-Version: 1.0
4, 29 -- Content-Type: text/plain; charset=UTF-8
4, 29 -- References: {370209020.7409633.1455987304853.JavaMail.yahoo.ref-at-mail.yahoo.com}
4, 29 -- Content-Transfer-Encoding: 8bit
4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1KGtJqN017594
==============================End of - Headers==============================






From: oshel1pe-at-cmich.edu
Date: Sun, 21 Feb 2016 09:20:14 -0600
Subject: [Microscopy] question - Allergy to histological solvents?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Daniel,

I assume you're doing these steps in a fume hood. One thing we've found for people who are sensitive to solvent fumes are charcoal-lined dust masks. Search amazon.com for "charcoal filter masks". We use the 3M type (but NOT for the prices here - we got ours through Fisher Scientific).
So far, they've worked fine.

Looks like you've already tried the various xylene replacements.

Phil

Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

________________________________________
X-from: dblackburn2000-at-yahoo.com [dblackburn2000-at-yahoo.com]
Sent: Saturday, February 20, 2016 12:16 PM
To: Oshel, Philip Eugene

Hello

After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes. These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause dizziness and pounding headaches. This has made staining and coverslipping very difficult, even with hood. Can anyone recommend alternative solutions for (a) dewaxing and (b) coverslipping with Permount? thanks very much!

Daniel Blackburn
Biology, Trinity College (Hartford CT)



==============================Original Headers==============================
4, 29 -- From dblackburn2000-at-yahoo.com Sat Feb 20 10:55:19 2016
4, 29 -- Received: from nm13-vm0.bullet.mail.bf1.yahoo.com (nm13-vm0.bullet.mail.bf1.yahoo.com [98.139.213.79])
4, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1KGtJqN017594
4, 29 -- for {Microscopy-at-microscopy.com} ; Sat, 20 Feb 2016 10:55:19 -0600
4, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1455987305; bh=GYAjj76GzomM7vop6UUnJyXkz0u+wZUkwa3SDd0g15A=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=TPxqPNC/tvQBHsTKLGuQ51Jxg7PrRk1Z77IjDYAtNrZPBfyLnjxprl57gqSAmcE6keQ080eKlTjoR/clH40sa3IPkQqVdl3HUZaugXs9TzpNOF+PJyPx7Ey2G68vm51CkrE0pfnoYku9ZaNs+8dasZxwEoJOt5N4tyekwNeyVH4GCpXRv4EFgmxNTtJtzt5Z6fJ9cGdjkoEooZibBz86jBWN70VTdga00AoibIBFXh/0g4qvxNfJKsxYB/XhmCgB/ZG4fK8She7vD2xu2k/W1HqNDBSK77t/7eLBoeuKid0158KLd6UXCr9j7zSErsJ0xwuMBw9LmRrmi9iC10fEHg==
4, 29 -- Received: from [66.196.81.170] by nm13.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000
4, 29 -- Received: from [98.139.212.222] by tm16.bullet.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000
4, 29 -- Received: from [127.0.0.1] by omp1031.mail.bf1.yahoo.com with NNFMP; 20 Feb 2016 16:55:05 -0000
4, 29 -- X-Yahoo-Newman-Property: ymail-3
4, 29 -- X-Yahoo-Newman-Id: 532123.73121.bm-at-omp1031.mail.bf1.yahoo.com
4, 29 -- X-YMail-OSG: pQ6H50EVM1mOAEYBONpigG3IZYomqCLazwII_MkU0fjcMa3_mrHLGMVY9za3Cb5
4, 29 -- rtfU9TxN46HHIjZZmoXVODUSN2PFdj3kF9Ae_nSuAc71MZ2azIzwmB4U4FJaEynMUv_j1mX4wReD
4, 29 -- mREMmAAZnMyIZTZuaQekhHvng_BLwBMnJ5vL9WPtVQxYrY2f1mGvDO4yzQZ31iy_AKLJtY9aZ2hV
4, 29 -- CeSPnr1ZrqqTnIR1XTfmWUi4OObTKxkXdcRKYUc0J7cKTEjtonO5nAK5yoXFFcZrxWTjUHSaQl8q
4, 29 -- 5E2S3qBWMCAyAOkd8im.uNmteAlj7usrcZXcQvG4cRrGBi8zNdGCptRXt2.2knijRUVID_.fcHWl
4, 29 -- 4XVdFdmAJirUe0hNywHM6LYT87WdteIDhiNyWHloS5g3DIr8jFKjILiRsuMQqSAiR.ERWgXqlZvj
4, 29 -- rvi0eS7x7hsidXf0j7fHi6aGnkElT8D8cxLc0Ivs2n9rouVgLONbz
4, 29 -- Received: by 76.13.27.50; Sat, 20 Feb 2016 16:55:05 +0000
4, 29 -- Date: Sat, 20 Feb 2016 16:55:04 +0000 (UTC)
4, 29 -- From: daniel blackburn {dblackburn2000-at-yahoo.com}
4, 29 -- Reply-To: daniel blackburn {dblackburn2000-at-yahoo.com}
4, 29 -- To: {Microscopy-at-microscopy.com}
4, 29 -- Message-ID: {370209020.7409633.1455987304853.JavaMail.yahoo-at-mail.yahoo.com}
4, 29 -- Subject: question - Allergy to histological solvents?
4, 29 -- MIME-Version: 1.0
4, 29 -- Content-Type: text/plain; charset=UTF-8
4, 29 -- References: {370209020.7409633.1455987304853.JavaMail.yahoo.ref-at-mail.yahoo.com}
4, 29 -- Content-Transfer-Encoding: 8bit
4, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1KGtJqN017594
==============================End of - Headers==============================


==============================Original Headers==============================
14, 35 -- From oshel1pe-at-cmich.edu Sun Feb 21 09:20:13 2016
14, 35 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
14, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1LFKCwi014223
14, 35 -- for {microscopy-at-microscopy.com} ; Sun, 21 Feb 2016 09:20:13 -0600
14, 35 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged))
14, 35 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u1LFJvtI005485;
14, 35 -- Sun, 21 Feb 2016 10:19:57 -0500
14, 35 -- Received: from cmail106.central.cmich.local ([fe80::a8:3e31:c722:be0a]) by
14, 35 -- cas2.central.cmich.local ([fe80::c44b:39ae:767:cab7%10]) with mapi id
14, 35 -- 14.03.0248.002; Sun, 21 Feb 2016 10:19:57 -0500
14, 35 -- From: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu}
14, 35 -- To: "dblackburn2000-at-yahoo.com" {dblackburn2000-at-yahoo.com}
14, 35 -- CC: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
14, 35 -- Subject: RE: [Microscopy] question - Allergy to histological solvents?
14, 35 -- Thread-Topic: [Microscopy] question - Allergy to histological solvents?
14, 35 -- Thread-Index: AQHRbAJzvIVVjB+UVkiX60gI5zJcWJ82nTv9
14, 35 -- Date: Sun, 21 Feb 2016 15:19:57 +0000
14, 35 -- Message-ID: {FB5ED57CF3415D4F8C5F93F1DE5468732FCE720F-at-cmail106.central.cmich.local}
14, 35 -- References: {201602201716.u1KHGmXC002359-at-ns.microscopy.com}
14, 35 -- In-Reply-To: {201602201716.u1KHGmXC002359-at-ns.microscopy.com}
14, 35 -- Accept-Language: en-US
14, 35 -- Content-Language: en-US
14, 35 -- X-MS-Has-Attach:
14, 35 -- X-MS-TNEF-Correlator:
14, 35 -- x-originating-ip: [141.209.2.100]
14, 35 -- Content-Type: text/plain; charset="us-ascii"
14, 35 -- MIME-Version: 1.0
14, 35 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
14, 35 -- X-Spam-Score: -6.00 () [Hold at 6.00] L_CTCMUM,L_EXCH_MF,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5)
14, 35 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
14, 35 -- X-CanItPRO-Stream: default
14, 35 -- X-Canit-Stats-ID: 05Ql3jVMv - 2337180cc265 - 20160221
14, 35 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116
14, 35 -- Content-Transfer-Encoding: 8bit
14, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1LFKCwi014223
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Sun, 21 Feb 2016 14:53:15 -0600
Subject: [Microscopy] viaWWW:Job Opportunity: Senior Analytical Technologist

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: q98-at-daycommunications.ca

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both q98-at-daycommunications.ca as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: q98-at-daycommunications.ca
Name: Human Resources

Organization: Ontario Ministry of the Environment and Climate Change

Title-Subject: [Filtered] Job Opportunity: Senior Analytical Technologist

Message: Erase Senior Analytical Technologist
Are you a flexible and versatile chemist with passion for investigating a wide range of inorganic,
particulate and organic substances?
If so, bring your knowledge and skills to the Ministry of the Environment and Climate Change, to
support environmental programs through monitoring, emergency response and litigation.
What can I expect to do in this role?
In this role, you will:
-i carry out moderate to highly complex analysis and identification of inorganic and organic material
in a wide array of environmental matrices
-i coordinate workload within the group and provide leadership to junior technologists to ensure
turn-around time is met
-i implement new and improved methods for the preparation, analysis and reporting of sample analysis
-i provide joint problem solution with customers and colleagues in the response to non-routine
environmental investigations
Location: Etobicoke
How do I qualify?
Knowledge of Laboratory Equipment and Systems:
-i You have current knowledge of and practical experience with the development, commissioning,
maintenance, and repair of advanced automated analytical laboratory instrumentation, including
optical and electron microscopy, x-ray fluorescence, x-ray diffraction, infra-red and UV-VIS
spectroscopy
-i You have practical experience with information management systems and their relationship to
laboratory data processing to ensure that data are correctly stored in the database and transmitted
to clients
-i You can operate, maintain and troubleshoot automated and semi-automated equipment
Knowledge of Sample Preparation:
-i You have knowledge of and practical experience with inorganic and organic analytical chemistry,
particulate analysis chemistry and identification methods related to a variety of environmental
sample matrices, including water, sewage, sediments, soil and air filters
-i You can use your knowledge to utilize laboratory methods that provide complementing types of data
-i You have knowledge of sampling methods and sample preservation procedures to provide advice
regarding proper sampling and sample preservation
Communication and Interpersonal Skills:
-i You have good oral and written communication and interpersonal skills
-i You are able to provide guidance to technologists and clients and prepare client reports and
document methods
-i You can communicate and confer with customers, including establishing data quality objectives,
interpreting data, joint problem solution and education of customers
-i You can provide factual testimony as to accuracy and precision of results, technology functions,
limitations and other qualities in court during legal proceedings
-i You are able to provide guidance, oversee work of junior staff and train others
-i You are capable of establishing effective working relationships with scientific, technical,
administrative, management co-workers and a diverse customer community
Analytical and Evaluative Skills:
-i You can select standard or alternative testing procedures, including both quantitative or
semi-quantitative methods and qualitative identification
-i You can troubleshoot problems related to sampling, testing and analysis of unusual samples
-i You can assess data quality against established criteria or in the absence of established criteria
against accepted scientific principles
-i You confer with publications, internet as well as external and external experts when confronted
with unusual samples
-i You know the principles of quality control and assurance, including ISO/IEC 17025
-i You know and can apply statistics to evaluate viability of new analytical methods
Salary Range: $1,216.18 - $1,443.88 per week
Additional information:
-i 1 Permanent, 125 Resources Rd, Etobicoke, Toronto Region
Please apply online, only, at www.ontario.ca/careers, quoting Job ID 89210, by Friday, March 4,
2016. Please follow the instructions to submit your application. Faxes are not being accepted at
this time.
If you require accommodation in order to participate in the recruitment process, please contact us
at www.gojobs.gov.on.ca/ContactUs.aspx to provide your contact information. Recruitment Services
staff will contact you within 48 hours. Only those applicants selected for an interview will be
contacted.
The Ontario Public Service is an inclusive employer. Accommodation will be provided in accordance
with Ontario-is Human Rights Code.
www.ontario.ca/careers
this text and type your question here

Login Host: 184.94.58.226
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
12, 53 -- From microscopy.listserver-at-gmail.com Sun Feb 21 14:53:15 2016
12, 53 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171])
12, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1LKrFrr021987
12, 53 -- for {microscopy-at-microscopy.com} ; Sun, 21 Feb 2016 14:53:15 -0600
12, 53 -- Received: by mail-ig0-f171.google.com with SMTP id hb3so65239818igb.0
12, 53 -- for {microscopy-at-microscopy.com} ; Sun, 21 Feb 2016 12:53:01 -0800 (PST)
12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 53 -- d=gmail.com; s=20120113;
12, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
12, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
12, 53 -- bh=yDHLxzElsHGciaM/WwDW5IEms/6/A9824uFOPtpt36U=;
12, 53 -- b=lKciccHIaoC1adOKPAgYZobONhnwRdtk2ahwP1M7hKZBAuZEK0KMdG8HjD8aRaPerK
12, 53 -- dBgWqDp0GB6Vc9ak89k2JBAO8nQoMHOBIv/vpX82XUav38W1VZl+JXrInl2SSsclLnDj
12, 53 -- Um0F185vJ5oo4YRY+NzNVUTCo86da54qk0Yyo/Z0QApW47NzGrntcBgspve9DN2qrSUW
12, 53 -- E+B+Qrhn08jkkYqzz8udtITvK/R8I9iBBqkDrb8ZeN8Nlo2/pCz2rEsxFOvQQsHYDr7J
12, 53 -- W6F2u8hyiMpXsTZm/srI4lXfmvZCln0Dc2lwKDRDGyPsi1JEpkJ5cY2/9kOqdYsqLUFp
12, 53 -- F8FA==
12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 53 -- d=1e100.net; s=20130820;
12, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
12, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
12, 53 -- :content-transfer-encoding;
12, 53 -- bh=yDHLxzElsHGciaM/WwDW5IEms/6/A9824uFOPtpt36U=;
12, 53 -- b=LCy1iiCSwCYMAmJNR6LqmLal/vF513zRuWIa4vAugHqZatRqCnX8zOlB0kwzD65YbK
12, 53 -- 0baWJdVMOtcXvrozFqBboMoD9GpUoRxChvvE0Semwlnc3oV0Hwy78MofS1cjgPCEVqhB
12, 53 -- M3rgJeeJK+5DQkpR4isK0ZUMQXaoE4vQ+UPNYu4RUKKflMjPV5BD+7PGdFkeyQ/Ei4EI
12, 53 -- xJ4bpCSwMrLnYqp/6S+OgQzsD1Y2rvxKhn2yYyt13RQY3wT3SRqlEUAcmwW9OoqQAa7w
12, 53 -- oFFcAajaNG7QIM+URDtidDrM9gpI5VxWCB5WH5iBMFRABbgGeI5dYR/38pBZOuVnzpcH
12, 53 -- isBg==
12, 53 -- X-Gm-Message-State: AG10YOSqqG2nHCmx0MfwXn7XvqThxxPlbJXBNmE6z5eWXlTQCbbPVkwS6/OOzCju3a9QJg==
12, 53 -- X-Received: by 10.50.92.41 with SMTP id cj9mr7971792igb.38.1456087980903;
12, 53 -- Sun, 21 Feb 2016 12:53:00 -0800 (PST)
12, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
12, 53 -- by smtp.googlemail.com with ESMTPSA id 78sm10274273iof.22.2016.02.21.12.52.59
12, 53 -- for {microscopy-at-microscopy.com}
12, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 53 -- Sun, 21 Feb 2016 12:53:00 -0800 (PST)
12, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
12, 53 -- {microscopylistserver-noreply-at-microscopy.com}
12, 53 -- Subject: viaWWW:Job Opportunity: Senior Analytical Technologist
12, 53 -- References: {201602192029.u1JKTj4a015862-at-ns.microscopy.com}
12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
12, 53 -- X-Forwarded-Message-Id: {201602192029.u1JKTj4a015862-at-ns.microscopy.com}
12, 53 -- Message-ID: {56CA23AB.4090501-at-microscopy.com}
12, 53 -- Date: Sun, 21 Feb 2016 14:52:59 -0600
12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
12, 53 -- Gecko/20100101 Thunderbird/38.6.0
12, 53 -- MIME-Version: 1.0
12, 53 -- In-Reply-To: {201602192029.u1JKTj4a015862-at-ns.microscopy.com}
12, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed
12, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Mon, 22 Feb 2016 06:04:06 -0600
Subject: [Microscopy] viaWWW:Cambridge S360 SEM Computer Error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Daniel,

A serious problem.
By listing, I have come with suggestions.
I have long used a Gum Damar formulation that I cannot offer, because I have prepared it in xylene.

NOTE: for those who might be interested - or users of Damar - I have a liter that I will NEVER use more than a few ml's over my reasonably extended age - now 76. Further, I don't do paraffin any more.

The suggestions:

If you can arrive at a solvent for Damar that is miscible with xylene/toluene, then it would be possible that you could prepare your own Damar. NOTE: Damar applied in the 1960's still have no sign of drying/cracking/crystalizing.
Source: http://www.eco-house.com/shop/950-damar-resin-crystals-graded/

You might test a hydrophilic mountant.
Source: https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx

If your allergy is broad-band, then you may have to resort to the latter. If it is more specific - just for those you mentioned - then your options might include Canada Balsam that is very natural, but also often found with benzene or xylene or toulene. I have some that will never be used as it requires some solvent that might not raise a sneeze.

Email me directly if you wish to discuss further,

Frederick C Monson, PhD
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pannsylvania
West Chester, PA, 1938
610-738-0437
fmonson-at-wcupa.edu

________________________________________
X-from: daniel blackburn via Histonet {histonet-at-lists.utsouthwestern.edu}
Sent: Saturday, February 20, 2016 2:44 PM
To: Histonet-at-lists.utsouthwestern.edu

X-from: mp76ers-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both mp76ers-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: mp76ers-at-gmail.com
Name: Mojahed

Organization: MERC

Title-Subject: [Filtered] Cambridge S360 SEM Computer Error

Message: Hi to all,
I have a problem with the microcomputer (SBC) of an old s360 SEM. It's model is PME 68-2 (or PME
68-12). It has s1-s8 LEDs on its front panel for error checking. Now s5 LED indicate en error. Does
anyone have its manual or know what does it mean?

Login Host: 78.109.195.113
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
11, 53 -- From microscopy.listserver-at-gmail.com Mon Feb 22 06:04:06 2016
11, 53 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176])
11, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MC46x4009565
11, 53 -- for {microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 06:04:06 -0600
11, 53 -- Received: by mail-ig0-f176.google.com with SMTP id 5so80000053igt.0
11, 53 -- for {microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 04:03:52 -0800 (PST)
11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 53 -- d=gmail.com; s=20120113;
11, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
11, 53 -- :mime-version:in-reply-to:content-type:content-transfer-encoding;
11, 53 -- bh=CKT9WQrdf695n8DeufztBur+EvwEJZunAQg4/qeDuMc=;
11, 53 -- b=u9s1d9IjkE4lwigA7/WkP0hqkuE1N/tilG5lqGrrodsYgXnF+LIthHp2dhr/SIaAZ+
11, 53 -- ziIcEm3vSUfQZatULgiTGnfNB8UzVUj/3WuOzZF3c5cLlSBV7lhgFSP84Z7XveFpQO8v
11, 53 -- bCRb9M9VWsZwQTIbkO+F/aLHOxUAqygi13qZM+H+v/oGsrX9JMcFAvQkUZcTwQ9vUxq7
11, 53 -- qnrZ6LuqrBxfrhj08Zl4kAw7T0ki2Xads/Elup3qJL+kBn02v/pG3BHRYJ7ygolmn5Nd
11, 53 -- o+qSCtlQxJwmM6UyFZrQenTRp2CDwq4Elj2i43e/gcswhEexKT4hZbFLRnlXc2g6lXaJ
11, 53 -- OOJw==
11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
11, 53 -- d=1e100.net; s=20130820;
11, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
11, 53 -- :date:user-agent:mime-version:in-reply-to:content-type
11, 53 -- :content-transfer-encoding;
11, 53 -- bh=CKT9WQrdf695n8DeufztBur+EvwEJZunAQg4/qeDuMc=;
11, 53 -- b=V6QqMYeoQrs3dbgajrMaJfqRUls/9QGa5Dg0bgXCRs9dVErxWwUGekRo25Z7BIm9ai
11, 53 -- hVrxHmGNE+T2NrIov3TmPWDfZuEO5j4Ua4+jS7eaJmbaYUmldNRmKFGCY+RwIRYaeCPI
11, 53 -- BQV8mA8fMa97K1cike1CSHeY7LGNTxT1MWPeHVxnOdaWy+tHobpxHxWWyje5uYpctPhF
11, 53 -- a1UtkaotSySe2Pg5xxcBKSzCO8Kl90PU/YAX+dXf0zAqVQzmM7O5s8nO6StRF0MnfCUD
11, 53 -- kr33n6SX0qtn+tkOqCEqFi6m3b1inF3UhL+YNbmlyYseUUSWxoyFJewNokRhJrC78zwg
11, 53 -- sPXQ==
11, 53 -- X-Gm-Message-State: AG10YOTVF523SF13IQo/qUlNeUJUnZgymqZCiCKTBXqP/x/Nww/AvQ+BY1skmkiLIaLMqQ==
11, 53 -- X-Received: by 10.50.20.98 with SMTP id m2mr7920571ige.9.1456142632268;
11, 53 -- Mon, 22 Feb 2016 04:03:52 -0800 (PST)
11, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
11, 53 -- by smtp.googlemail.com with ESMTPSA id x1sm9143433iga.12.2016.02.22.04.03.51
11, 53 -- for {microscopy-at-microscopy.com}
11, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
11, 53 -- Mon, 22 Feb 2016 04:03:51 -0800 (PST)
11, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
11, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
11, 53 -- {microscopylistserver-noreply-at-microscopy.com}
11, 53 -- Subject: viaWWW:Cambridge S360 SEM Computer Error
11, 53 -- References: {201602221033.u1MAX8PC004489-at-ns.microscopy.com}
11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
11, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
11, 53 -- X-Forwarded-Message-Id: {201602221033.u1MAX8PC004489-at-ns.microscopy.com}
11, 53 -- Message-ID: {56CAF926.40709-at-microscopy.com}
11, 53 -- Date: Mon, 22 Feb 2016 06:03:50 -0600
11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
11, 53 -- Gecko/20100101 Thunderbird/38.6.0
11, 53 -- MIME-Version: 1.0
11, 53 -- In-Reply-To: {201602221033.u1MAX8PC004489-at-ns.microscopy.com}
11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: tbargar-at-unmc.edu
Date: Mon, 22 Feb 2016 08:30:10 -0600
Subject: [Microscopy] Users of FEI TeneoVS SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

I would like to hear from anyone out there who is using FEI's TeneoVS SEM. I want to know your experiences with it and I would like to get a sample run as a demo, we can cover any associated costs.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



==============================Original Headers==============================
7, 37 -- From tbargar-at-unmc.edu Mon Feb 22 08:30:10 2016
7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126])
7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MEUA1r001242
7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:30:10 -0600
7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1])
7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id EAA6F5C5E75
7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:07:31 -0600 (CST)
7, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1])
7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
7, 37 -- (No client certificate requested)
7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id 02E195C5E6A
7, 37 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:07:31 -0600 (CST)
7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by
7, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id
7, 37 -- 14.03.0235.001; Mon, 22 Feb 2016 08:29:55 -0600
7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu}
7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 37 -- Subject: Users of FEI TeneoVS SEM
7, 37 -- Thread-Topic: Users of FEI TeneoVS SEM
7, 37 -- Thread-Index: AdFtfSnkLKYP9x9wRmufl/4nk4jZUQ==
7, 37 -- Date: Mon, 22 Feb 2016 14:29:53 +0000
7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE854050-at-UNMCEX2.unmcresforest.org}
7, 37 -- Accept-Language: en-US
7, 37 -- Content-Language: en-US
7, 37 -- X-MS-Has-Attach:
7, 37 -- X-MS-TNEF-Correlator:
7, 37 -- x-originating-ip: [10.8.64.15]
7, 37 -- Content-Type: text/plain; charset="us-ascii"
7, 37 -- MIME-Version: 1.0
7, 37 -- X-VPM-MSG-ID: 22c5e17d-fb11-48c5-a02c-43161552b8cd
7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu
7, 37 -- X-VPM-GROUP-ID: 668ad697-ab40-4dca-8a37-267e6a56aa92
7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext
7, 37 -- X-VPM-CERT-FLAG: 0
7, 37 -- X-VPM-IS-HYBRID: 0
7, 37 -- Content-Transfer-Encoding: 8bit
7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1MEUA1r001242
==============================End of - Headers==============================




From: Winston.Wiggins-at-cshs.org
Date: Mon, 22 Feb 2016 10:09:23 -0600
Subject: [Microscopy] RE: question - Allergy to histological solvents?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Unfortunately, you,Aeove probably lost your sense of smell and taste if you,Aeove become that sensitized. I would suggest the quickest remedy may be using a half-mask respirator with organic vapor filter cartridges, even though you may be using a fume hood. I think paint respirators may work since they filter organics/solvents/paints. Make sure it fits correctly too. It won't do to have a proper filtering assembly if it doesn't fit correctly!
Good luck.
~Winston

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Winston W. Wiggins, E.M. Pathology Specialist
CEDARS-SINAI MEDICAL CENTER, Pathology & Lab Medicine
Electron Microscopy, LLSPT, A828
8700 Beverly Blvd.
Los Angeles, CA 90048-1865
OAess310-423-1363 (off); 310-423-5323 (lab); 310-423-0685 (fax)
Winston.Wiggins-at-CSHS.org
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation.


==============================Original Headers==============================
5, 50 -- From winston.wiggins-at-cshs.org Mon Feb 22 10:09:23 2016
5, 50 -- Received: from espxlsmg04.csmc.edu (mx4.csmc.edu [192.231.86.201])
5, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MG9Mof024718
5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 10:09:23 -0600
5, 50 -- X-AuditID: 0a200141-f790b6d00000272b-9e-56cb32a01a48
5, 50 -- Received: from CSHSMSGZIX04.CSMC.EDU (cshsmsgzix04.csmc.edu [10.17.158.163])
5, 50 -- by espxlsmg04.csmc.edu (CS) with SMTP id 7C.A0.10027.0A23BC65; Mon, 22 Feb 2016 08:09:04 -0800 (PST)
5, 50 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1])
5, 50 -- by Outbound.cshs.org (Proprietary) with SMTP id 0B0DC7203FD
5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:04:56 -0800 (PST)
5, 50 -- Received: from ESPWMSGHUB05.CSMC.EDU (espwmsghub05.csmc.edu [10.17.239.90])
5, 50 -- by CSHSMSGZIX04.CSMC.EDU (Proprietary) with ESMTP id D42CD7203FB
5, 50 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 08:04:55 -0800 (PST)
5, 50 -- Received: from CSHSMSGMBX01.CSMC.EDU ([169.254.3.125]) by
5, 50 -- ESPWMSGHUB05.CSMC.EDU ([10.17.239.90]) with mapi id 14.03.0235.001; Mon, 22
5, 50 -- Feb 2016 08:09:08 -0800
5, 50 -- From: "Wiggins, Winston" {Winston.Wiggins-at-cshs.org}
5, 50 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
5, 50 -- Subject: RE: question - Allergy to histological solvents?
5, 50 -- Thread-Topic: RE: question - Allergy to histological solvents?
5, 50 -- Thread-Index: AdFtiEeekRFJ5XMDSxmYMvDNpL2XNw==
5, 50 -- Date: Mon, 22 Feb 2016 16:09:07 +0000
5, 50 -- Message-ID: {77186365EA63114782772AB6D4940EEE7A76049A-at-cshsmsgmbx01.CSMC.EDU}
5, 50 -- Accept-Language: en-US
5, 50 -- Content-Language: en-US
5, 50 -- X-MS-Has-Attach:
5, 50 -- X-MS-TNEF-Correlator:
5, 50 -- x-originating-ip: [166.124.28.190]
5, 50 -- Content-Type: text/plain; charset="utf-8"
5, 50 -- MIME-Version: 1.0
5, 50 -- X-VPM-MSG-ID: ecee1c8c-3893-4e58-a917-854ca910f97f
5, 50 -- X-VPM-HOST: CSHSMSGZIX04.CSMC.EDU
5, 50 -- X-VPM-GROUP-ID: f17f7004-8acd-4c98-922c-6736a276b9ca
5, 50 -- X-VPM-ENC-REGIME: Plaintext
5, 50 -- X-VPM-CERT-FLAG: 0
5, 50 -- X-VPM-IS-HYBRID: 0
5, 50 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFtrKIsWRmVeSWpSXmKPExsXCJThvse4Co9NhBu+mC1j8OdzA7sDo8bH7
5, 50 -- NVMAYxSXTUpqTmZZapG+XQJXxr5nU9gKNvFVfJvv18DYwNfFyMEhIWAi8XRSaBcjJ5ApJnHh
5, 50 -- 3nq2LkYuDiGBzYwSKw//ZoRwtjBKnHm2HMq5zijRe2obE4SzkFHiRd9qJpBRbALGEu/W5IKM
5, 50 -- EhGwlfh24Q4TiC0sYCkx/+kmRoi4ncS5viUsELaexKbJp8DiLAKqEidfHmEFsXkFfCT6bnYx
5, 50 -- g9iMQCd9P7UGbA6zgLjErSfzmSBOFZBYsuc8M4QtKvHy8T9WCFtJYt36i2wg5zALaEqs36UP
5, 50 -- 0aooMaX7ITvEeEGJkzOfsECUG0nseHmRHcJWlNhz7h8bhG0i8X7jSai4tMSqF3NZIIElJHH7
5, 50 -- lBZEWEji7Il7rBMYpWchOW4WwuJZSBbPQrJ4ASPLKkbh1OKCipzi3HQDE73k4txkvdSU0k2M
5, 50 -- oMhUYHTcwTjhku0hRgEORiUeXgPOU2FCrIllxZW5hxglOJiVRHjrpE6HCfGmJFZWpRblxxeV
5, 50 -- 5qQWH2KU5mBREuft3nw8TEggPbEkNTs1tSC1CCbLxMEp1cDYXx7+Vfbnh9k++qw24QziSvn/
5, 50 -- Ju3defxFk9rOP50n6wqV5kvVeUZYF5VccLZY7nGmZdIWrmVRWc6xpSJMl19tXiKu/N09JDrx
5, 50 -- +j8RqwAvo3tznX7xC3zQ4pQ39Xgj+f3C9nY3uWnnSlWXK6gdkVdhtPW68HtNk1XohFU5vRNk
5, 50 -- KjS3TY1/+VmJpTgj0VCLuag4EQAiRscByAIAAA==
5, 50 -- Content-Transfer-Encoding: 8bit
5, 50 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1MG9Mof024718
==============================End of - Headers==============================




From: frank_karl-at-ardl.com
Date: Wed, 24 Feb 2016 07:07:25 -0600
Subject: [Microscopy] Fiber and Fabric

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Mississippi State University's College of Engineering will be hosting a Research Experience for Undergraduates May 31-Aug. 6.

Research will be in Materials Science and Engineering and will include topics such as biomaterials, nanoparticles, composites & polymers, and nanocrystalline materials. The program will also include professional and development seminars, industry site visits, and GRE preparation workshops.

The REU includes a weekly stipend, on-campus housing, and a meal allowance. For more information and/or to apply on-line please visit the college of engineering website: www.bagley.msstate.edu/REU/.

Amanda Lawrence
Outreach Coordinator/Research Associate
Institute for Imaging and Analytical Technologies
Mississippi State University




==============================Original Headers==============================
7, 33 -- From ALawrence-at-i2at.msstate.edu Mon Feb 22 16:04:05 2016
7, 33 -- Received: from chokecherry.its.msstate.edu (chokecherry.its.msstate.edu [130.18.2.120])
7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1MM45OT025147
7, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 16:04:05 -0600
7, 33 -- Received: from mail06.ad.msstate.edu (mail06.ad.msstate.edu [130.18.230.65])
7, 33 -- by chokecherry.its.msstate.edu (8.13.8/8.13.8) with ESMTP id u1MM3pgk011042
7, 33 -- (version=TLSv1/SSLv3 cipher=AES256-SHA bits=256 verify=FAIL)
7, 33 -- for {Microscopy-at-microscopy.com} ; Mon, 22 Feb 2016 16:03:51 -0600
7, 33 -- X-Sender: {}
7, 33 -- Received: from MAIL02.ad.msstate.edu (2002:8212:e63d::8212:e63d) by
7, 33 -- mail06.ad.msstate.edu (2002:8212:e641::8212:e641) with Microsoft SMTP Server
7, 33 -- (TLS) id 15.0.913.22; Mon, 22 Feb 2016 16:03:50 -0600
7, 33 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by
7, 33 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id
7, 33 -- 15.00.0913.011; Mon, 22 Feb 2016 16:03:50 -0600
7, 33 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu}
7, 33 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
7, 33 -- Subject: summer research experience for engineering and materials science
7, 33 -- undergraduates
7, 33 -- Thread-Topic: summer research experience for engineering and materials
7, 33 -- science undergraduates
7, 33 -- Thread-Index: AdFtvC1M/yUDJOBSSVSKwPnrpzqTTg==
7, 33 -- Date: Mon, 22 Feb 2016 22:03:50 +0000
7, 33 -- Message-ID: {485c84d1773740c2856dbc8514520eb2-at-mail02.ad.msstate.edu}
7, 33 -- Accept-Language: en-US
7, 33 -- Content-Language: en-US
7, 33 -- X-MS-Has-Attach:
7, 33 -- X-MS-TNEF-Correlator:
7, 33 -- x-originating-ip: [130.18.230.93]
7, 33 -- Content-Type: text/plain; charset="us-ascii"
7, 33 -- MIME-Version: 1.0
7, 33 -- Content-Transfer-Encoding: 8bit
7, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1MM45OT025147
==============================End of - Headers==============================

From advertise.bz222dcedu-at-gmail.com Wed Feb 24 00:57:18 2016
Return-Path: {advertise.bz222dcedu-at-gmail.com}
Received: from gmail.com ([121.78.112.171])
by ns.microscopy.com (8.12.11.20060308/8.12.8) with SMTP id u1O6vFHM030289
for {microscopylistserverarchive5-at-microscopy.com} ; Wed, 24 Feb 2016 00:57:16 -0600
Message-ID: {2EC237BE.15C3727C-at-gmail.com}

In 1921, Ms.Valencia Arton took for her second term, summer quarter a course called Textiles at the University of Chicago. She constructed a lab book consisting of experiments, typed and long hand notes and samples of fabric before and after testing.

I've found her notebook over 30 years ago in a used bookstore thinking, "What a source of old fabric samples and fibers" but I never did anything with it.

I'm sure Ms. Arton is long past caring, but I can't help but think some microscopist could find a publication in these pages, if they look hard enough. Valencia would probably enjoy that.

The book is free to anyone who would want it, no strings attached. She does mention the microscope and it is an interesting look at the analytical fiber and fabric procedures of 1921.

Contact me if you have question or need more detail.........

Frank
ARDL


This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.



==============================Original Headers==============================
10, 27 -- From frank_karl-at-ardl.com Wed Feb 24 07:07:25 2016
10, 27 -- Received: from cal1-mh746b.smtproutes.com (cal1-mh746b.smtproutes.com [208.70.89.157])
10, 27 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OD7OdY006599
10, 27 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 07:07:24 -0600
10, 27 -- X-Katharion-ID: 1456319214.50048.cal1-mh746
10, 27 -- Received: from mail.ardl.com ([98.100.51.26]) by
10, 27 -- cal1-mh746.smtproutes.com [(192.69.16.68)] with ESMTP via TCP
10, 27 -- (TLSv1/TLS_RSA_WITH_AES_128_CBC_SHA); 24 Feb 2016 13:06:54 +0000
10, 27 -- Received: from exchange2k7.ad.ardl.com ([fe80::596e:af10:621c:b9f8]) by
10, 27 -- exchange2k7.ad.ardl.com ([fe80::596e:af10:621c:b9f8%14]) with mapi; Wed, 24
10, 27 -- Feb 2016 08:06:54 -0500
10, 27 -- From: Frank Karl {frank_karl-at-ardl.com}
10, 27 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
10, 27 -- Date: Wed, 24 Feb 2016 08:06:53 -0500
10, 27 -- Subject: Fiber and Fabric
10, 27 -- Thread-Topic: Fiber and Fabric
10, 27 -- Thread-Index: AdFvBDt1tIQveEdOS+ORmMZWmyaU5A==
10, 27 -- Message-ID: {0445DA8FFC235F4CBDAF9BACCF79E12108C792C0-at-exchange2k7.ad.ardl.com}
10, 27 -- Accept-Language: en-US
10, 27 -- Content-Language: en-US
10, 27 -- X-MS-Has-Attach:
10, 27 -- X-MS-TNEF-Correlator:
10, 27 -- acceptlanguage: en-US
10, 27 -- Content-Type: text/plain; charset="us-ascii"
10, 27 -- MIME-Version: 1.0
10, 27 -- Content-Transfer-Encoding: 8bit
10, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1OD7OdY006599
==============================End of - Headers==============================




From: apamatos-at-gmail.com
Date: Wed, 24 Feb 2016 08:08:12 -0600
Subject: [Microscopy] Extension of the deadline for abstract submission to Ultrapath XVIII

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Organizing Committee of Ultrapath XVIII
(http://congress.ultrapathxviii.org) has extended the deadline for
abstract submission to March 24, 2016.
Your work is very important for the Society and your contribution to the
Congress will help all of us to keep improving and spread the word about
the continued importance of Ultrastructural Pathology.

With kind regards,

From the Organizing Committee,

A.P. Alves de Matos
Chair of UltrapathXVIII


==============================Original Headers==============================
6, 46 -- From apamatos-at-gmail.com Wed Feb 24 08:08:11 2016
6, 46 -- Received: from mail-wm0-f52.google.com (mail-wm0-f52.google.com [74.125.82.52])
6, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OE8BfF028573
6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 08:08:11 -0600
6, 46 -- Received: by mail-wm0-f52.google.com with SMTP id g62so6243159wme.0
6, 46 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 06:07:57 -0800 (PST)
6, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 46 -- d=gmail.com; s=20120113;
6, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version
6, 46 -- :content-transfer-encoding;
6, 46 -- bh=jGwYeYU2P9NVDSHXIC/ikbUi66LcT+w0CW3CNixU668=;
6, 46 -- b=AyWnZTkk64GX2E3ViGo4aeetpSqKRZBYdShbpzeUvbrrLEnLqFCZ8CyiDB2wmIZIzl
6, 46 -- 5VLSMTW2ztw8zgVsCZAjXDC+q2Uh6+j1qOseUMeO19zhZKAIWM19oqLR5Bnlvym07Fzs
6, 46 -- XUj4E46nj4nHpWiVWpVvmASogYRUGtLjyppPVZ1FufqulY9odLuIagYASTAwHDHuKHZd
6, 46 -- tglqfmWGzHUcB5Oqq6uB2GruiClY9DfkhG8tM43YJ0C9Zgi0L3nMpm0rLH7CGrATXndm
6, 46 -- qkld+/dCBHFU3J+paFA6OcZtAeVj80Ot+fPWqKF5dqGz/vBXIYG8q7y8NUmzbGCpCjB2
6, 46 -- LZoQ==
6, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 46 -- d=1e100.net; s=20130820;
6, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent
6, 46 -- :mime-version:content-transfer-encoding;
6, 46 -- bh=jGwYeYU2P9NVDSHXIC/ikbUi66LcT+w0CW3CNixU668=;
6, 46 -- b=RwpqrBKKMRzYx+dbOnsaxl+pkpC57UpteKuRJSo/ikl//4ZdxV9TWcdRPX5+qqitm+
6, 46 -- In+biXygyuua0qWor3+IInJgcTobaGhjDVoE04iKr/cEtxxBbCO2ZGctbqM5f2KXwo1r
6, 46 -- digaDiPDAw9493jqxOMvXR6ZAIKSav5DVZZ+XH83+6RSejSeYkX0t38rH6VE3KhdN2sL
6, 46 -- hL/K5R1zCaY+/Et3oEBzUSYyIhf/reJXhz1az5E/4rj+C+IMrpCvuKTX30eOhnh2HNYA
6, 46 -- SGmX/ELYZp5NBY3cLqYNkAKNVCc/NTT/1C57e6UBmJEj8Wuyi6rIxP3a1KwubHcmEARJ
6, 46 -- 1K0Q==
6, 46 -- X-Gm-Message-State: AG10YOS50eWzhmbrpT34bvFzMofZ1s8ECgiP2LfRp851Ipps+eHzjsHHK2gBtLW91GMe3A==
6, 46 -- X-Received: by 10.28.14.140 with SMTP id 134mr23384508wmo.39.1456322876619;
6, 46 -- Wed, 24 Feb 2016 06:07:56 -0800 (PST)
6, 46 -- Received: from ?IPv6:2001:8a0:f946:ec01:4261:86ff:fe2b:8e36? ([2001:8a0:f946:ec01:4261:86ff:fe2b:8e36])
6, 46 -- by smtp.gmail.com with ESMTPSA id gb9sm3204146wjb.26.2016.02.24.06.07.53
6, 46 -- for {microscopy-at-microscopy.com}
6, 46 -- (version=TLSv1/SSLv3 cipher=OTHER);
6, 46 -- Wed, 24 Feb 2016 06:07:54 -0800 (PST)
6, 46 -- To: microscopy-at-microscopy.com
6, 46 -- From: AP Alves de Matos {apamatos-at-gmail.com}
6, 46 -- Subject: Extension of the deadline for abstract submission to Ultrapath XVIII
6, 46 -- Message-ID: {56CDB939.6020308-at-gmail.com}
6, 46 -- Date: Wed, 24 Feb 2016 14:07:53 +0000
6, 46 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:38.0) Gecko/20100101
6, 46 -- Thunderbird/38.6.0
6, 46 -- MIME-Version: 1.0
6, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed
6, 46 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================




From: ph2-at-sprynet.com
Date: Wed, 24 Feb 2016 09:21:30 -0600
Subject: [Microscopy] Call for Papers - Inter/Micro 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Call for Papers - Register Online Today

Inter/Micro 2016

http://www.mcri.org/v/101/intermicro

An international microscopy conference.
June 6 - 10 at McCrone Research Institute, Chicago


Join some of the world's leading amateur and professional microscopists for
the 68th annual Inter/Micro conference, featuring:
. Research presentations on techniques/instrumentation,
industrial/environmental microscopy, and chemical/
forensic microscopy
. Two-day workshop on microscopy of sand, taught by Thomas J. Hopen of the
ATF
. Evening with Brian Ford presentation: "Magical Mystery Tour of the Cell"
. State Microscopical Society of Illinois Awards Dinner
. ... and more! View the schedule of events.

Research presentations will be held June 6 - 8. Speakers will receive a $50
registration discount. Read abstract submission guidelines.

Use our secure online registration form at www.mcri.org to reserve your seat
today.

We look forward to seeing you in Chicago!


McCrone Research Institute
A Not-for-Profit Corporation
2820 South Michigan Avenue, Chicago, IL 60616-3230
Phone: 312-842-7100 Fax: 312-842-1078
www.mcri.org
intermicro-at-mcri.org

.........................................
Andrew Anthony "Tony" Havics, CIH, PE
Environmental, Health & Safety, Microscopy, Materials Science & Forensic
Engineering
pH2, LLC
5250 E US Highway 36, Suite 830
Avon, IN 46123
(317) 718-7020 Office
(317) 718-7038 Fax
(317) 409-3238 Cell
aahavics-at-pH2LLC.com
www.ph2LLC.com

This message is from pH2. This message and any attachments may contain
legally privileged or confidential information, and are intended only for
the individual or entity identified above as the addressee. If you are not
the addressee, or if this message has been addressed to you in error, you
are not authorized to read, copy, or distribute this message and any
attachments, and we ask that you please delete this message and attachments
(including all copies) and notify the sender by return e-mail or by phone at
317-718-7020. Delivery of this message and any attachments to any person
other than the intended recipient(s) is not intended in any way to waive
confidentiality or a privilege. All personal messages express views only of
the sender, which are not to be attributed to pH2 and may not be copied or
distributed without this statement.




==============================Original Headers==============================
13, 26 -- From ph2-at-sprynet.com Wed Feb 24 09:21:30 2016
13, 26 -- Received: from elasmtp-masked.atl.sa.earthlink.net (elasmtp-masked.atl.sa.earthlink.net [209.86.89.68])
13, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OFLUcR018529
13, 26 -- for {microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 09:21:30 -0600
13, 26 -- DomainKey-Signature: a=rsa-sha1; q=dns; c=nofws;
13, 26 -- s=dk20050327; d=sprynet.com;
13, 26 -- b=Pt9RaMKcujcxSM58oP5jH3zXPJNwCIPl9wJnr5H2Xka2ymRIbFSVZGHyLwmaINyv;
13, 26 -- h=Received:From:To:Subject:Date:Message-ID:MIME-Version:Content-Type:Content-Transfer-Encoding:X-Mailer:Thread-Index:Content-Language:X-ELNK-Trace:X-Originating-IP;
13, 26 -- Received: from [68.58.112.163] (helo=AAHHPdv7)
13, 26 -- by elasmtp-masked.atl.sa.earthlink.net with esmtpa (Exim 4.67)
13, 26 -- (envelope-from {ph2-at-sprynet.com} )
13, 26 -- id 1aYbFR-0001Mw-68; Wed, 24 Feb 2016 10:21:05 -0500
13, 26 -- From: "Tony Havics, CHMM, CIH, PE" {ph2-at-sprynet.com}
13, 26 -- To: "Microscopy Listserve" {microscopy-at-microscopy.com}
13, 26 -- Subject: Call for Papers - Inter/Micro 2016
13, 26 -- Date: Wed, 24 Feb 2016 10:21:04 -0500
13, 26 -- Message-ID: {002301d16f16$fa107040$ee3150c0$-at-sprynet.com}
13, 26 -- MIME-Version: 1.0
13, 26 -- Content-Type: text/plain;
13, 26 -- charset="us-ascii"
13, 26 -- Content-Transfer-Encoding: 7bit
13, 26 -- X-Mailer: Microsoft Outlook 14.0
13, 26 -- Thread-Index: AdFvFobPmzC92okxRByiyEo5xFxtfA==
13, 26 -- Content-Language: en-us
13, 26 -- X-ELNK-Trace: 6888e50b2be9b4fee5331016acda17f99cb45db0fb6b68c30031faad74a9ee41350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c350badd9bab72f9c
13, 26 -- X-Originating-IP: 68.58.112.163
==============================End of - Headers==============================




From: tbargar-at-unmc.edu
Date: Wed, 24 Feb 2016 11:15:11 -0600
Subject: [Microscopy] Free Denton Vacuum Evaporator DV-502

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We are getting rid of our Denton Vacuum Evaporator DV-502. If anyone out there would like a free vacuum evaporator you are welcome to come and get it. Our lab is located at UNMC in Omaha, Nebraska.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



==============================Original Headers==============================
6, 37 -- From tbargar-at-unmc.edu Wed Feb 24 11:15:11 2016
6, 37 -- Received: from zixvpm02.unmc.edu (mysecuremail.unmc.edu [192.198.54.127])
6, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1OHFBjx009521
6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 11:15:11 -0600
6, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1])
6, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id C5E4638520B
6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 10:55:53 -0600 (CST)
6, 37 -- Received: from UNMCEX1.unmcresforest.org (unknown [10.8.3.1])
6, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
6, 37 -- (No client certificate requested)
6, 37 -- by zixvpm02.unmc.edu (Proprietary) with ESMTPS id BE04B38520A
6, 37 -- for {Microscopy-at-microscopy.com} ; Wed, 24 Feb 2016 10:55:52 -0600 (CST)
6, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by
6, 37 -- UNMCEX1.unmcresforest.org ([fe80::2023:ee90:3fae:f0d6%12]) with mapi id
6, 37 -- 14.03.0235.001; Wed, 24 Feb 2016 11:14:53 -0600
6, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu}
6, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
6, 37 -- Subject: Free Denton Vacuum Evaporator DV-502
6, 37 -- Thread-Topic: Free Denton Vacuum Evaporator DV-502
6, 37 -- Thread-Index: AdFvJn+TTJy4rbS3ToC6BhV9WE0Qtw==
6, 37 -- Date: Wed, 24 Feb 2016 17:14:53 +0000
6, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE854567-at-UNMCEX2.unmcresforest.org}
6, 37 -- Accept-Language: en-US
6, 37 -- Content-Language: en-US
6, 37 -- X-MS-Has-Attach:
6, 37 -- X-MS-TNEF-Correlator:
6, 37 -- x-originating-ip: [10.8.64.15]
6, 37 -- Content-Type: text/plain; charset="us-ascii"
6, 37 -- MIME-Version: 1.0
6, 37 -- X-VPM-MSG-ID: e26c73e3-4d1f-4bba-8481-375263525094
6, 37 -- X-VPM-HOST: zixvpm02.unmc.edu
6, 37 -- X-VPM-GROUP-ID: 9a1af4c2-901d-4ff8-951f-23599a9bbf45
6, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext
6, 37 -- X-VPM-CERT-FLAG: 0
6, 37 -- X-VPM-IS-HYBRID: 0
6, 37 -- Content-Transfer-Encoding: 8bit
6, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1OHFBjx009521
==============================End of - Headers==============================







From: tbargar-at-unmc.edu
Date: Thu, 25 Feb 2016 10:27:11 -0600
Subject: [Microscopy] would like to hear from Tescan customers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear listers,

I would like to hear privately from anyone using Tescan SEM and especially anyone who is using a Tescan with the integrated Gatan ultramicrotome for serial block face imaging. I'm not familiar with this company, so all feedback would be appreciated.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.



==============================Original Headers==============================
7, 37 -- From tbargar-at-unmc.edu Thu Feb 25 10:27:10 2016
7, 37 -- Received: from zixvpm01.unmc.edu (zixvpm01.unmc.edu [192.198.54.126])
7, 37 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1PGRAaF005835
7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:27:10 -0600
7, 37 -- Received: from 127.0.0.1 (ZixVPM [127.0.0.1])
7, 37 -- by Outbound.unmc.edu (Proprietary) with SMTP id C11455C6181
7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:04:19 -0600 (CST)
7, 37 -- Received: from UNMCEX3.unmcresforest.org (unknown [10.8.3.3])
7, 37 -- (using TLSv1 with cipher AES128-SHA (128/128 bits))
7, 37 -- (No client certificate requested)
7, 37 -- by zixvpm01.unmc.edu (Proprietary) with ESMTPS id D36265C6173
7, 37 -- for {Microscopy-at-microscopy.com} ; Thu, 25 Feb 2016 10:04:18 -0600 (CST)
7, 37 -- Received: from UNMCEX2.unmcresforest.org ([fe80::151d:89d:49b6:a0ab]) by
7, 37 -- UNMCEX3.unmcresforest.org ([fe80::9840:65e3:a657:ff93%13]) with mapi id
7, 37 -- 14.03.0235.001; Thu, 25 Feb 2016 10:26:55 -0600
7, 37 -- From: "Bargar, Tom W" {tbargar-at-unmc.edu}
7, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 37 -- Subject: would like to hear from Tescan customers
7, 37 -- Thread-Topic: would like to hear from Tescan customers
7, 37 -- Thread-Index: AdFv6Owu3Y0nkb+lTEWu6gBu4tHd9g==
7, 37 -- Date: Thu, 25 Feb 2016 16:26:54 +0000
7, 37 -- Message-ID: {E5858BD0583CA8499131AC34AD6766B0AE8549F1-at-UNMCEX2.unmcresforest.org}
7, 37 -- Accept-Language: en-US
7, 37 -- Content-Language: en-US
7, 37 -- X-MS-Has-Attach:
7, 37 -- X-MS-TNEF-Correlator:
7, 37 -- x-originating-ip: [10.8.64.15]
7, 37 -- Content-Type: text/plain; charset="us-ascii"
7, 37 -- MIME-Version: 1.0
7, 37 -- X-VPM-MSG-ID: e96d80d7-3a70-4386-a1c0-04b659029cc0
7, 37 -- X-VPM-HOST: zixvpm01.unmc.edu
7, 37 -- X-VPM-GROUP-ID: 0d5882fa-6463-4dbf-b262-a87cdbd7a309
7, 37 -- X-VPM-ENC-REGIME: ZixVPM,Plaintext
7, 37 -- X-VPM-CERT-FLAG: 0
7, 37 -- X-VPM-IS-HYBRID: 0
7, 37 -- Content-Transfer-Encoding: 8bit
7, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1PGRAaF005835
==============================End of - Headers==============================




From: Z.Zhou-at-lboro.ac.uk
Date: Fri, 26 Feb 2016 08:23:05 -0600
Subject: [Microscopy] Microscopy facility online booking systems

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fellow Microscopists,

What are the typical software systems you use to manage on-line booking to use scopes or sample prep facilities? Either commercially available or free systems are of my interest. I would be extremely grateful if you would please share with me the experiences and expectations you have had.
The system we use now allows on-line booking of a dozen resources from users, notifying users in advance, managing the cost of the usage automatically, etc. However, it's not embedded within individual equipment. The booking and the usage is not electronically linked. Ideally when a booking is made to use a scope, and this user account will implement the booking by logging in the facility on the session. Therefore the booking and the real usage will be directly linked with a true picture. Anybody is using such a system now?

Best regards,
Dr Z Zhou
Loughborough University, UK


==============================Original Headers==============================
4, 62 -- From Z.Zhou-at-lboro.ac.uk Fri Feb 26 08:23:05 2016
4, 62 -- Received: from mta-1.lboro.ac.uk (mta-1.lut.ac.uk [158.125.160.47])
4, 62 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1QEN4xS031468
4, 62 -- for {Microscopy-at-microscopy.com} ; Fri, 26 Feb 2016 08:23:05 -0600
4, 62 -- Received: from [158.125.167.11] (helo=ITSCASH-4.lunet.lboro.ac.uk)
4, 62 -- by mta-1.lboro.ac.uk with esmtps (TLSv1.2:ECDHE-RSA-AES256-SHA384:256)
4, 62 -- (Exim 4.86)
4, 62 -- id 1aZJI2-0003KK-IE
4, 62 -- for Microscopy-at-microscopy.com; Fri, 26 Feb 2016 14:22:42 +0000
4, 62 -- Received: from EUR01-HE1-obe.outbound.protection.outlook.com (213.199.154.207)
4, 62 -- by email.lboro.ac.uk (158.125.167.4) with Microsoft SMTP Server (TLS) id
4, 62 -- 14.3.266.1; Fri, 26 Feb 2016 14:23:01 +0000
4, 62 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=lunet.onmicrosoft.com;
4, 62 -- s=selector1-lboro-ac-uk;
4, 62 -- h=From:To:Date:Subject:Message-ID:Content-Type:MIME-Version;
4, 62 -- bh=MD7V5z7CrshoU91IPfML5frRF2LdNReDvC7jsfqpGHg=;
4, 62 -- b=wCWw9T7NrBo0QVR7qzJGx1BMXdWynqI0/r1Y5bexQCtgXWuGa0BNR1tYZJz3dv1+C8/KqKDQsm0Iy9EPYHVLuakQ2kO6DzJvT/wk0mUHXTSjv0f7lEDV87cg+JWdSJRdGdQ9fi/6QZOIxJrTV5BoVTFY3qAZaZFAOqWMAstg2bw=
4, 62 -- Received: from AM3PR04MB0614.eurprd04.prod.outlook.com
4, 62 -- (2a01:111:e400:8832::20) by AM3PR04MB0613.eurprd04.prod.outlook.com
4, 62 -- (2a01:111:e400:8832::19) with Microsoft SMTP Server (TLS) id 15.1.415.20;
4, 62 -- Fri, 26 Feb 2016 14:22:41 +0000
4, 62 -- Received: from AM3PR04MB0614.eurprd04.prod.outlook.com
4, 62 -- ([fe80::8df7:f5c3:cd34:f616]) by AM3PR04MB0614.eurprd04.prod.outlook.com
4, 62 -- ([fe80::8df7:f5c3:cd34:f616%18]) with mapi id 15.01.0415.022; Fri, 26 Feb
4, 62 -- 2016 14:22:41 +0000
4, 62 -- From: Zhaoxia Zhou {Z.Zhou-at-lboro.ac.uk}
4, 62 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
4, 62 -- Subject: Microscopy facility online booking systems
4, 62 -- Thread-Topic: Microscopy facility online booking systems
4, 62 -- Thread-Index: AdFwn+DmixrpHErMTCmGa0M03+Hxag==
4, 62 -- Date: Fri, 26 Feb 2016 14:22:41 +0000
4, 62 -- Message-ID: {AM3PR04MB06146FB4EA10966058A3C19FC6A70-at-AM3PR04MB0614.eurprd04.prod.outlook.com}
4, 62 -- Accept-Language: en-GB, en-US
4, 62 -- Content-Language: en-US
4, 62 -- X-MS-Has-Attach:
4, 62 -- X-MS-TNEF-Correlator:
4, 62 -- authentication-results: microscopy.com; dkim=none (message not signed)
4, 62 -- header.d=none;microscopy.com; dmarc=none action=none header.from=lboro.ac.uk;
4, 62 -- x-originating-ip: [2001:630:301:3064:e91b:4846:16ec:e893]
4, 62 -- x-microsoft-exchange-diagnostics: 1;AM3PR04MB0613;5:2iX7jEWZa2OjO/XTI5089OBmqFTGVgqOUeUXLMTtztXlLMXY9tcvR3M83JAs2Ayx4B4LIE6EIrvF0C8ImL9ZylYyynRPMFzrEuazSn8kgm9zOM61/EghUZhz8dqs512wIekpZbp6l/+YRt9SvA/Ong==;24:1UXtmER9ytEchx/eXP+4sO6wMrkUXhMRIQD91rdACzvXg1GlJUbgtN4KEWu5tZYQd9XvVx2BRQsBLTtxm0hDuHUupadkcDbfkAzuHFN2W8Q=
4, 62 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:AM3PR04MB0613;
4, 62 -- x-ms-office365-filtering-correlation-id: cb8f763a-4ac2-4b93-ef98-08d33eb8495c
4, 62 -- x-microsoft-antispam-prvs: {AM3PR04MB06138FA27DB456A5414C52E0C6A70-at-AM3PR04MB0613.eurprd04.prod.outlook.com}
4, 62 -- x-exchange-antispam-report-test: UriScan:;
4, 62 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046);SRVR:AM3PR04MB0613;BCL:0;PCL:0;RULEID:;SRVR:AM3PR04MB0613;
4, 62 -- x-forefront-prvs: 0864A36BBF
4, 62 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10019020)(6009001)(74482002)(87936001)(86362001)(40100003)(2351001)(229853001)(92566002)(2900100001)(5002640100001)(1220700001)(1096002)(5003600100002)(3660700001)(586003)(102836003)(6116002)(5250100002)(2501003)(33656002)(50986999)(11100500001)(54356999)(3280700002)(5008740100001)(76576001)(450100001)(5001960100002)(189998001)(5004730100002)(2906002)(107886002)(110136002)(74316001)(3826002);DIR:OUT;SFP:1102;SCL:1;SRVR:AM3PR04MB0613;H:AM3PR04MB0614.eurprd04.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en;
4, 62 -- spamdiagnosticoutput: 1:23
4, 62 -- spamdiagnosticmetadata: NSPM
4, 62 -- Content-Type: text/plain; charset="utf-8"
4, 62 -- MIME-Version: 1.0
4, 62 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 26 Feb 2016 14:22:41.2760
4, 62 -- (UTC)
4, 62 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted
4, 62 -- X-MS-Exchange-CrossTenant-id: cf264fc0-aeb8-449f-9054-82ce4454084b
4, 62 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: AM3PR04MB0613
4, 62 -- X-OriginatorOrg: lboro.ac.uk
4, 62 -- X-Scan-Signature: 924a1c3e3a3d639a1424bf4a1917f89b
4, 62 -- X-Lboro-Creds: scanned on mta-1.lboro.ac.uk
4, 62 -- X-Lboro-Filtered: mta-1.lboro.ac.uk, Fri, 26 Feb 2016 14:22:42 +0000
4, 62 -- Content-Transfer-Encoding: 8bit
4, 62 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u1QEN4xS031468
==============================End of - Headers==============================




From: henning.stahlberg-at-unibas.ch
Date: Sat, 27 Feb 2016 03:26:19 -0600
Subject: [Microscopy] PostDoc in cryo-EM and image processing in Basel, Switzerland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


A PostDoc position is available in the BioEM Lab of the University of Basel, Switzerland.

We are looking for a person with experience in single particle cryo-EM and image processing, using RELION, FREALIGN, EMAN2, and other packages. The position is available immediately, and funded initially for two years, with the possibility for multi-year extension and possible transition into a permanent position. The role of this person should be to perform cryo-EM sample preparation and cryo-EM data collection, and perform advanced image processing and 3D reconstructions. Expertise in single particle image analysis is required.
Several high-impact biological projects are waiting, including studying larger membrane protein complexes by cryo-EM as single particles, as well as helical assemblies.

The person should serve as liaison between the BioEM Lab, which is a newly established high-resolution cryo-EM service facility at the the Center for Cellular Imaging and NanoAnalytics of the Univeristy of Basel, and the laboratory of biomolecular research at the Paul-Scherrer Institute (PSI) in Villigen. An interaction with collaborators and clients from the pharmaceutical industry interested in membrane protein structure determination is also foreseen.

This position involves high-resolution cryo-EM structural work on membrane proteins, for which our FEI Titan Krios (Quantum-LS GIF / K2 Summit) and the FEI Polara (K2 Summit) are outstanding instruments. We also operate a number of additional instruments for sample screening and cryo-EM grid analysis, including a FEI Talos, CM200FEG, T12, CM100, CM10, Versa3D, and others. Large computer clusters are available. The lab atmosphere is especially nice, and the city of Basel in Switzerland at the border to Germany and France has a beautiful historic center and a rich culture, and provides a high standard of living. This is a good place to live and to do science.

If you are interested, please contact Henning.Stahlberg-at-unibas.ch for further infos.


Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62




==============================Original Headers==============================
11, 35 -- From henning.stahlberg-at-unibas.ch Sat Feb 27 03:26:18 2016
11, 35 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164])
11, 35 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1R9QHmM021105
11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 03:26:18 -0600
11, 35 -- Received: from localhost (localhost [127.0.0.1])
11, 35 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id B15F23E0171
11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 10:26:02 +0100 (CET)
11, 35 -- X-Virus-Scanned: amavisd-new at unibas.ch
11, 35 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164])
11, 35 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024)
11, 35 -- with LMTP id psnNyuW6_AVh for {Microscopy-at-microscopy.com} ;
11, 35 -- Sat, 27 Feb 2016 10:26:02 +0100 (CET)
11, 35 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131])
11, 35 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id A1F133E0185
11, 35 -- for {Microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 10:26:02 +0100 (CET)
11, 35 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by
11, 35 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id
11, 35 -- 14.03.0266.001; Sat, 27 Feb 2016 10:26:02 +0100
11, 35 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
11, 35 -- To: Microscopy {Microscopy-at-microscopy.com}
11, 35 -- Subject: PostDoc in cryo-EM and image processing in Basel, Switzerland
11, 35 -- Thread-Topic: PostDoc in cryo-EM and image processing in Basel, Switzerland
11, 35 -- Thread-Index: AQHRcUDfmNVu3pRwuU6BdfawFhkB5Q==
11, 35 -- Date: Sat, 27 Feb 2016 09:26:01 +0000
11, 35 -- Message-ID: {5C666C53-43FE-408E-A676-74146D262FF7-at-unibas.ch}
11, 35 -- Accept-Language: en-US, de-CH
11, 35 -- Content-Language: en-US
11, 35 -- X-MS-Has-Attach:
11, 35 -- X-MS-TNEF-Correlator:
11, 35 -- x-originating-ip: [131.152.226.242]
11, 35 -- Content-Type: text/plain; charset="us-ascii"
11, 35 -- Content-ID: {EBFB11FC3A4BAF469B9A3584707F0FAC-at-zuv.ads.unibas.ch}
11, 35 -- MIME-Version: 1.0
11, 35 -- Content-Transfer-Encoding: 8bit
11, 35 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u1R9QHmM021105
==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Sat, 27 Feb 2016 17:36:16 -0600
Subject: [Microscopy] viaWWW:Biological X-ray Analysis with SEM EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: htowbin-at-amnh.org

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when
replying
please copy both htowbin-at-amnh.org as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: htowbin-at-amnh.org
Name: Henry Towbin

Organization: American Museum of Natural History

Title-Subject: [Filtered] Biological X-ray Analysis with SEM EDS

Message: Hi All,
Can anyone provide some referenced for good biological thin film x-ray
analysis? I am particularly interested in how to prepare good standards
for using the Hall-Marshal or similar method to analyze C, N and P.
I am trying to help some colleague with analyzing the compositions of
singles celled organisms with x-ray analysis in an SEM.
They are attempting to dry the cells on a formvar support and plan to
measure counts from the cell and the film and then subtract the counts
of the film to calculate composition. I am highly skeptical of the
methods they want to use from Norland et al. 1995 (Light Element
Analysis of Individual Bacteria by X-Ray Microanalysis), so any advice
would be much appreciated.
Thank you,
Henry

Norland et al. 1995, Light Element Analysis of Individual Bacteria by
X-Ray Microanalysis, http://aem.asm.org/content/61/4/1357.full.pdf
______________________________
Henry Towbin
Laboratory Co-manager
Microscopy and Imaging Facility
American Museum of Natural History
79th St & Central Park West
NY, NY 10024

Login Host: 69.115.216.78
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================

==============================Original Headers==============================
13, 50 -- From microscopy.listserver-at-gmail.com Sat Feb 27 17:36:15 2016
13, 50 -- Received: from mail-pf0-f169.google.com (mail-pf0-f169.google.com [209.85.192.169])
13, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1RNaFFD005533
13, 50 -- for {microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 17:36:15 -0600
13, 50 -- Received: by mail-pf0-f169.google.com with SMTP id t66so4425591pfb.3
13, 50 -- for {microscopy-at-microscopy.com} ; Sat, 27 Feb 2016 15:36:01 -0800 (PST)
13, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 50 -- d=gmail.com; s=20120113;
13, 50 -- h=subject:references:to:reply-to:from:message-id:date:user-agent
13, 50 -- :mime-version:in-reply-to:content-transfer-encoding;
13, 50 -- bh=gngyq0N2JxX6zknYc1arZc5pK/zw8cAkFoQKrZOPjWs=;
13, 50 -- b=CL90GEsFoWA7OPqiDjTkJHoIxz4bxyhI3qprFjPoFVmRyf280QB4QgAUK7klNxY25U
13, 50 -- ZSumCRcTTI4r9muq7Tqm1NiHuUZ4qlUvmDBu96u1R+WDyLYNmDAJsRA+iNh1z4QbAyCl
13, 50 -- vOz45+shYcjj7bsyFb528snBiNnCmhg6OPsdAnCKy8D2AevkC8jdV0cI6KNu4MDLZOPl
13, 50 -- h45tpLVoTHWBE1CAfc+F9h2mep6tO2KwIPbzODNHUTGJg7Ee4pXMDmtBhfjlEJ47Jzjj
13, 50 -- //jobFSjj5E1hehe6oQJfINXH8Rbm0robcp+KFuMFZjHUmPXYzQOzDMG5eZd0ycFXEWv
13, 50 -- nWdw==
13, 50 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 50 -- d=1e100.net; s=20130820;
13, 50 -- h=x-gm-message-state:subject:references:to:reply-to:from:message-id
13, 50 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
13, 50 -- bh=gngyq0N2JxX6zknYc1arZc5pK/zw8cAkFoQKrZOPjWs=;
13, 50 -- b=KrTx0xmdqgR3pBOKacs0GB2f/ucTc4j/Z7VTumTrW/1vhdfqv6lVOfrvgpyGOUTlAW
13, 50 -- hN5Ln/ifNgFa+UpNi1/yQndQNVRtpOChXZw6m96FdHc0YSnUz738jsC/DOkj9G6rOT3W
13, 50 -- zS+JIgdKbL0ogM6SNHwkkQeqcshID7fK9O5pRqhT0IrEk6fxOempD/zQ+4SPXWP8si1N
13, 50 -- syiOc/ZDZBSER9JzKX1dFkNZgJyBTmSO074jbAF2ojLsk6TS+fN+UEWAzEVPDJuLw+0i
13, 50 -- D9iPixW+d4bqj1TGe2nvAsnN9Z7NAmTM2oqR1zpnYLpRoSIyWiLpEeNQ832L+hrIeGKR
13, 50 -- MfAA==
13, 50 -- X-Gm-Message-State: AD7BkJKp/n9XLk+RDUqnfVPEYauUPUHe0ij72fM+HZX5hOhMIEV6lFD9GL8vHoJXYg3EmQ==
13, 50 -- X-Received: by 10.98.34.212 with SMTP id p81mr12056914pfj.23.1456616161046;
13, 50 -- Sat, 27 Feb 2016 15:36:01 -0800 (PST)
13, 50 -- Received: from Nestors-MacBookAir-Pro-2014.local ([207.158.132.69])
13, 50 -- by smtp.googlemail.com with ESMTPSA id q16sm28213131pfi.80.2016.02.27.15.35.58
13, 50 -- for {microscopy-at-microscopy.com}
13, 50 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 50 -- Sat, 27 Feb 2016 15:36:00 -0800 (PST)
13, 50 -- Subject: viaWWW:Biological X-ray Analysis with SEM EDS
13, 50 -- References: {201602272014.u1RKEmxn002305-at-ns.microscopy.com}
13, 50 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 50 -- Reply-To: microscopy.listserver-at-gmail.com
13, 50 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 50 -- X-Forwarded-Message-Id: {201602272014.u1RKEmxn002305-at-ns.microscopy.com}
13, 50 -- Message-ID: {56D23305.4070603-at-gmail.com}
13, 50 -- Date: Sat, 27 Feb 2016 18:36:37 -0500
13, 50 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 50 -- Gecko/20100101 Thunderbird/38.5.1
13, 50 -- MIME-Version: 1.0
13, 50 -- In-Reply-To: {201602272014.u1RKEmxn002305-at-ns.microscopy.com}
13, 50 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 50 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================






From: hlowers-at-usgs.gov
Date: Mon, 29 Feb 2016 15:59:48 -0600
Subject: [Microscopy] EPMA2016 Topical Conference and Student Support

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Announcing a MAS Topical Conference, Electron Probe Microanalysis 2016
May 16-19, 2016 at the University of Wisconsin-Madison

STUDENT FINANCIAL SUPPORT AVAILABLE!

TOPICAL CONFERENCE PROGRAM
-Practical microanalysis for beginners to professionals
-Plenary Conference including user meetings, tutorials, group
discussion, problem solving, and instrument demos
-Quantitative analysis using the electron microprobe and SEM with EDS and WDS
-Improvements in microanalysis from measurement to accuracy
-Trace element analysis
-Compositional x-ray mapping and cathodoluminescence
-Applications in earth science, materials science, and industry

STUDENT FINANCIAL SUPPORT AVAILABLE
-Early career scholars: undergraduate, graduate, postdoc, and early
career professionals may apply
-Priority will be given to those with co-sponsorship from a
university, advisor, or employer
-ECS awardees may submit a 2-page abstract for platform or poster presentation.
-Awardees will receive a maximum of $750 reimbursement for travel,
lodging, and registration.

DEADLINE MARCH 15, 2016 FOR ABSTRACTS AND ECS APPLICATIONS

Please visit for more information
http://www.microprobe.org/topical-conferences/epma-2016-1/epma-2016

Heather Lowers, EPMA 2016 ECS Coordinator
Paul Carpenter, EPMA 2016 Planning Chair

==============================Original Headers==============================
7, 74 -- From hlowers-at-usgs.gov Mon Feb 29 15:59:48 2016
7, 74 -- Received: from smtp5.doi.gov (SMTP5.doi.gov [137.227.28.11])
7, 74 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u1TLxm4Y000494
7, 74 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Feb 2016 15:59:48 -0600
7, 74 -- Received: from smtp6.smgr.doi.gov (10.10.28.12) by smtp5.doi.gov (10.10.28.21)
7, 74 -- with Microsoft SMTP Server (TLS) id 14.2.347.0; Mon, 29 Feb 2016 16:59:33
7, 74 -- -0500
7, 74 -- DKIM-Signature: v=1; a=rsa-sha256; d=usgs.gov; s=mail; c=relaxed/simple;
7, 74 -- q=dns/txt; i=-at-usgs.gov; t=1456783172; x=1459375172;
7, 74 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc:MIME-Version:Content-Type:
7, 74 -- Content-Transfer-Encoding:Content-ID:Content-Description:Resent-Date:Resent-From:
7, 74 -- Resent-Sender:Resent-To:Resent-Cc:Resent-Message-ID:In-Reply-To:References:List-Id:
7, 74 -- List-Help:List-Unsubscribe:List-Subscribe:List-Post:List-Owner:List-Archive;
7, 74 -- bh=AF83KI6d7MXpYJb22FVaEyDt5MAnhS0GKurGXjn8wps=;
7, 74 -- b=NXbZxK90Gg/Z5ZDlxN2DlqicJJBHcOT3R7MjaP0tPWXiXVKRE1n3prWVXFnNn7iy
7, 74 -- S/smRuuKhLmcXBOU/dqA7s9Bdyvnlj54/YPgkR3CfiP+oOsQWq+UOE24al0oWBz3
7, 74 -- GeqWoBqO+65oiSao/oj9rHyfmE15f2RknOF/la/osNY=;
7, 74 -- DKIM-Signature: v=1; a=rsa-sha256; d=usgs.gov; s=mail; c=relaxed/simple;
7, 74 -- q=dns/txt; i=-at-usgs.gov; t=1456783172; x=1459375172;
7, 74 -- h=From:Sender:Reply-To:Subject:Date:Message-ID:To:Cc:MIME-Version:Content-Type:
7, 74 -- Content-Transfer-Encoding:Content-ID:Content-Description:Resent-Date:Resent-From:
7, 74 -- Resent-Sender:Resent-To:Resent-Cc:Resent-Message-ID:In-Reply-To:References:List-Id:
7, 74 -- List-Help:List-Unsubscribe:List-Subscribe:List-Post:List-Owner:List-Archive;
7, 74 -- bh=AF83KI6d7MXpYJb22FVaEyDt5MAnhS0GKurGXjn8wps=;
7, 74 -- b=NXbZxK90Gg/Z5ZDlxN2DlqicJJBHcOT3R7MjaP0tPWXiXVKRE1n3prWVXFnNn7iy
7, 74 -- S/smRuuKhLmcXBOU/dqA7s9Bdyvnlj54/YPgkR3CfiP+oOsQWq+UOE24al0oWBz3
7, 74 -- GeqWoBqO+65oiSao/oj9rHyfmE15f2RknOF/la/osNY=;
7, 74 -- X-AuditID: 0a0a1c0c-f79db6d00000551c-c5-56d4bf440602
7, 74 -- Received: from gsmtp6.doi.gov (Unknown_Domain [10.10.28.16]) (using TLS with
7, 74 -- cipher AES128-SHA (128/128 bits)) (Client did not present a certificate) by
7, 74 -- smtp6.smgr.doi.gov (Hello) with SMTP id 05.9A.21788.44FB4D65; Mon, 29 Feb
7, 74 -- 2016 16:59:32 -0500 (EST)
7, 74 -- Received: from mail-vk0-f41.google.com (209.85.213.41) by gsmtp6.doi.gov
7, 74 -- (137.227.28.16) with Microsoft SMTP Server (TLS) id 14.2.347.0; Mon, 29 Feb
7, 74 -- 2016 16:59:15 -0500
7, 74 -- Received: by mail-vk0-f41.google.com with SMTP id c3so148740676vkb.3
7, 74 -- for {Microscopy-at-microscopy.com} ; Mon, 29 Feb 2016 13:59:27 -0800 (PST)
7, 74 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 74 -- d=1e100.net; s=20130820;
7, 74 -- h=x-gm-message-state:mime-version:date:message-id:subject:from:to;
7, 74 -- bh=AF83KI6d7MXpYJb22FVaEyDt5MAnhS0GKurGXjn8wps=;
7, 74 -- b=jf2BBwbpC5y3yeLqrHXovNnlyAGmYlilmhXqcF2+o2FgqDbxQNLP+DqQaKl3nfhq4D
7, 74 -- WQB1TRGBFCXLCA8oG5+4kfHKyEGGxoET1PsPhb3sxw32fkrJ74igwSS6HpzxDcmHVrm5
7, 74 -- wlVEsi1xRnCLlUVmxR7XldLvcbFgugrVk6a+u+VuQXVQtVMZ8bH1n008UPMSIEbt4EqG
7, 74 -- w+MNqAsn9AAf68AeSO6+cXcKbX0BKBymOMXauQxPVTF0k5ddRYMXCZ/6pwkaAQPa+dmI
7, 74 -- 92s7MsudmcmbgG+uIpuc2BCMyy/cR0NE/M0rBO8Cf+OReIf4LBNzNj/zB/4RTAhLAkUD
7, 74 -- NP2g==
7, 74 -- X-Gm-Message-State: AD7BkJKgxdZnlyYFjtqPiutQ3/bJnJZ5IaGT2VnwQtBcRrT35ALr5wx9mi4kMl1y/CMnaqjQcZvnnWoLdxQecW95CRKSVlLI6NNcFi4ZTw4QCTLwCctMiPKeFfGY1KdyPwvMbih1lhRT1gn1IEc2WxHn25GAfBVBfZOAxTbuiTcK7PieTw1FtQcVXZf634pzsX/wjPNOInUQ4Rum/dlcsYy14OGmW8+DEDwJbeZgBMpN
7, 74 -- X-Received: by 10.31.133.19 with SMTP id h19mr13404690vkd.127.1456783162359;
7, 74 -- Mon, 29 Feb 2016 13:59:22 -0800 (PST)
7, 74 -- MIME-Version: 1.0
7, 74 -- X-Received: by 10.31.133.19 with SMTP id h19mr13404651vkd.127.1456783161405;
7, 74 -- Mon, 29 Feb 2016 13:59:21 -0800 (PST)
7, 74 -- Received: by 10.31.228.129 with HTTP; Mon, 29 Feb 2016 13:59:21 -0800 (PST)
7, 74 -- Date: Mon, 29 Feb 2016 14:59:21 -0700
7, 74 -- Message-ID: {CAOx2-aBhNdsCafkayXbLwvLr+VvZpmsFvQ+8QjpeUG079Ftk6w-at-mail.gmail.com}
7, 74 -- Subject: EPMA2016 Topical Conference and Student Support
7, 74 -- From: "Lowers, Heather" {hlowers-at-usgs.gov}
7, 74 -- To: microscopy {Microscopy-at-microscopy.com}
7, 74 -- Content-Type: text/plain; charset="UTF-8"
7, 74 -- X-Gm-Spam: 0
7, 74 -- X-Gm-Phishy: 0
7, 74 -- X-CFilter-Loop: Reflected
7, 74 -- X-Brightmail-Tracker: H4sIAAAAAAAAA+NgFnrCLMWRmVeSWpSXmKPExsXCxfTjla7L/ithBlOXW1j8OdzA7sDo8bH7
7, 74 -- NVMAYxSXTUpqTmZZapG+XQJXxqV9HWwFK7gq1n/6ydzA+Jiji5GLg0VgC6vE2Y1X2LoYOTgk
7, 74 -- BEwkuvuVuhg5gUwxiQv31rOB1AgJNDFJTF59mwUkISSwhlFiytIciMQURolDO48zQXQ0skqc
7, 74 -- m+UBkpAQaGCSuDG3nQ0iUS0x6+l/VhCbV0BQ4uTMJywQ20ok3p4SgBjqLXH10jpmEJtFQFVi
7, 74 -- 66JJTBDlARJ90+aAjREWsJA4POU4mM0moCUx+fQxMFsEyG7r3ccOYjMLaEq0bv/NDrGWR2LK
7, 74 -- 3ItQNp/EmitboB6Qleib0MMCEZeUOLjiBssERrFZSK6bhWTUAkamVYxCxbklBWZ6xbnpRXop
7, 74 -- +Zl66fllmxhBYc8lw7ODcfFDi0OMAhyMSjy8H5ZfCRNiTSwrrsw9xCjBwawkwpu7DSjEm5JY
7, 74 -- WZValB9fVJqTWnyIUZqDRUmcN1/FKUxIID2xJDU7NbUgtQgmy8TBKdXAaPDUsyya5danmFWH
7, 74 -- 3/n+uOpTHZwbY79zkcJ5nUMvvimznnfc/UvmcZpx+MY7ZTq1paYdBzQFHsQeltJQCeA4uzZV
7, 74 -- p+Va54HO7pdFm7WTrq0+PrE/XkN3+h/z3V8ebrgUol1u/PrXzzcOnonvlm/84ijZt9UpLJjF
7, 74 -- w5xpQpDi6hu6fW++SW98qcRSnJFoqMVcVJwIAKb6UOl3AgAA
==============================End of - Headers==============================









From: microscopy.listserver-at-gmail.com
Date: Thu, 12 May 2016 07:00:52 -0500
Subject: [Microscopy] Administrivia: Nestor is doing testing Ignore this message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sorry All

Must do a bit of testing to restore functionality which
has broken.

Nestor



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================


==============================Original Headers==============================
10, 46 -- From microscopy.listserver-at-gmail.com Thu May 12 07:00:52 2016
10, 46 -- Received: from mail-io0-f170.google.com (mail-io0-f170.google.com [209.85.223.170])
10, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CC0qbd004807
10, 46 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 07:00:52 -0500
10, 46 -- Received: by mail-io0-f170.google.com with SMTP id f89so91292700ioi.0
10, 46 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 05:01:04 -0700 (PDT)
10, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 46 -- d=gmail.com; s=20120113;
10, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version
10, 46 -- :content-transfer-encoding;
10, 46 -- bh=ekJtcsYBinLLV7t/gEEJVQ5QOJADEcBy3NUO0cVnuZc=;
10, 46 -- b=GyJ+tGPGHDUEsAb0h7Yz0zKz+LqPghxuu95OSYlLw53G1YqC91iaJTcijDL+WgA0Kv
10, 46 -- jMwC4ecsatDV5OtR/HNKnAm2S/jT2svPclT8hqrbT3IoBOQvjKBVEBL7ov5sJnReg5Xq
10, 46 -- 7v3n1wFxYR9lr0HaUuE5VD18JshlfOppsmVKqtiFt2YcPQz517VYrRO69bQsAbK0uVvC
10, 46 -- 1bjNm8d08/ES+Mpk6E5h6gqRwaE+SWvBWjcfgBTV7DZ9HwZjrkXopEKaTAq3NOtWQ8k5
10, 46 -- N4UlcQ5qie2xCqjJIaPAD676u4E1/+gFYebV0hi0BxEfoYilKmV1VagBHKmPEj/MXORg
10, 46 -- C/TQ==
10, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 46 -- d=1e100.net; s=20130820;
10, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent
10, 46 -- :mime-version:content-transfer-encoding;
10, 46 -- bh=ekJtcsYBinLLV7t/gEEJVQ5QOJADEcBy3NUO0cVnuZc=;
10, 46 -- b=CloD5zseNhlgatGNH3lfRaqR3r/eAbyTPJ7noOtcmUFHTo8XQBbkbTWtX6+Ny3lDhM
10, 46 -- 7V4u338tAAPwNytx/SGPqnXUs3PaeO2t52AsP0iz0GcIIKR3cyBI/WxB7gqJNDWPRNFO
10, 46 -- DG05Xf4D2eyfQ5kX57Vs6hMzoeFg2qTD2yoTv+8ujU4V+bQmUFL2C+umcPK3yrMyRu6o
10, 46 -- UcNPwn2/n/sfNgMEEnVmSNJdIRt1oB9EcarlrXco58LBd6CXVizgOYy+Fa9oOtShNigG
10, 46 -- PCkQYN/JHmXieavK4lxgMOay590EQCJSics/ogMht83HhLaHAITIsw54td2YGmr9FkN1
10, 46 -- AXeA==
10, 46 -- X-Gm-Message-State: AOPr4FXwoyUmDhoZaft+gy+96rfJz3fJjkycQABtdDda7wNlWtgzDIzZXklqanZA5CJZfw==
10, 46 -- X-Received: by 10.107.186.6 with SMTP id k6mr7219720iof.136.1463054464304;
10, 46 -- Thu, 12 May 2016 05:01:04 -0700 (PDT)
10, 46 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
10, 46 -- by smtp.googlemail.com with ESMTPSA id qn2sm4458894igc.20.2016.05.12.05.01.03
10, 46 -- for {microscopy-at-microscopy.com}
10, 46 -- (version=TLSv1/SSLv3 cipher=OTHER);
10, 46 -- Thu, 12 May 2016 05:01:03 -0700 (PDT)
10, 46 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
10, 46 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com}
10, 46 -- Subject: Administrivia: Nestor is doing testing Ignore this message
10, 46 -- Message-ID: {40f1ba8c-3a0e-d29e-d491-1218a2688b3e-at-gmail.com}
10, 46 -- Date: Thu, 12 May 2016 07:01:03 -0500
10, 46 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
10, 46 -- Gecko/20100101 Thunderbird/45.0
10, 46 -- MIME-Version: 1.0
10, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed
10, 46 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================









From: yaroslav.tsybovsky-at-nih.gov
Date: Thu, 12 May 2016 12:04:23 -0500
Subject: [Microscopy] TEM: Research Associate position at Leidos Biomedical Research,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:

http://jobs.leidos.com/ShowJob/Id/832108/Research-Associate-I-628272-%28NCI%29/

Best regards,

Yaroslav Tsybovsky (Contractor)
Scientist
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc.
Post Office Box B
Frederick, Maryland 21702



==============================Original Headers==============================
7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016
7, 47 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111])
7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CH4NKn001027
7, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:04:23 -0500
7, 47 -- X-SBRS-Extended: Low
7, 47 -- X-IronPortListener: Outbound_SMTP
7, 47 -- IronPort-PHdr: =?us-ascii?q?9a23=3AGVHDpBbZhvUgcMpc3gRB9OH/LSx+4OfEezUN459i?=
7, 47 -- =?us-ascii?q?sYplN5qZpM66bnLW6fgltlLVR4KTs6sC0LqH9fmxEjVYvN6oizMrTt9lb1c9k8?=
7, 47 -- =?us-ascii?q?IYnggtUoauKHbQC7rUVRE8B9lIT1R//nu2YgB/Ecf6YEDO8DXptWZBUiv2OQc9?=
7, 47 -- =?us-ascii?q?HOnpAIma153xjLDjvcSCKFwQ2XKUWvBbElaflU3prM4YgI9veO4a6yDihT92Qd?=
7, 47 -- =?us-ascii?q?lQ3n5iPlmJnhzxtY+a9Z9n9DlM6bp6r5YTGZjge+wEaZAQTHF/ayFmrPHs4FPm?=
7, 47 -- =?us-ascii?q?TACV4WAXVX0Huh9JCBLC9xr9Roa3uSz//KIp/SiRJ8rtRrcsSByn7qxxTwTjjz?=
7, 47 -- =?us-ascii?q?8WcTU+9TeEpNZ3ifcRnAmwrRth2I3FJMmkPeB5ZafUY5taY0thGeB6dm0JMaiJ?=
7, 47 -- =?us-ascii?q?JcNbHuMbOv1cppe7u0AfpxygHgq9LOXuynlHgWGgjv5y6PgoDQyThF9oJNkJqn?=
7, 47 -- =?us-ascii?q?mB6YytOQ=3D=3D?=
7, 47 -- X-IronPort-Anti-Spam-Filtered: true
7, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENg?=
7, 47 -- =?us-ascii?q?XYkhXACgTY4FAEBAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTq?=
7, 47 -- =?us-ascii?q?bZIYmiQyDKYIuBZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBA?=
7, 47 -- =?us-ascii?q?Q?=
7, 47 -- X-IPAS-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENgXYkhXACgTY4FAE?=
7, 47 -- =?us-ascii?q?BAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTqbZIYmiQyDKYIuB?=
7, 47 -- =?us-ascii?q?ZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBAQ?=
7, 47 -- X-IronPort-AV: E=Sophos;i="5.24,610,1454994000";
7, 47 -- d="scan'208";a="459727113"
7, 47 -- Received: from unknown (HELO msgb10.nih.gov) ([156.40.71.130])
7, 47 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES256-SHA; 12 May 2016 13:04:02 -0400
7, 47 -- Received: from MSGB01.nih.gov ([169.254.1.154]) by msgb10.nih.gov
7, 47 -- ([169.254.10.4]) with mapi id 14.03.0279.002; Thu, 12 May 2016 13:04:02 -0400
7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov}
7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research,
7, 47 -- Inc./Frederick National Laboratory for Cancer Research
7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical
7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research
7, 47 -- Thread-Index: AdGscBobBV8ZFKKkTvmemwETdQ92zg==
7, 47 -- Date: Thu, 12 May 2016 17:04:01 +0000
7, 47 -- Message-ID: {AEB1E864C83965459AE2D8A2139E1B2DD13FE7-at-msgb01.nih.gov}
7, 47 -- Accept-Language: en-US
7, 47 -- Content-Language: en-US
7, 47 -- X-MS-Has-Attach:
7, 47 -- X-MS-TNEF-Correlator:
7, 47 -- x-originating-ip: [156.40.70.6]
7, 47 -- Content-Type: text/plain; charset="us-ascii"
7, 47 -- MIME-Version: 1.0
7, 47 -- Content-Transfer-Encoding: 8bit
7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CH4NKn001027
==============================End of - Headers==============================










From: yaroslav.tsybovsky-at-nih.gov
Date: Thu, 12 May 2016 12:04:23 -0500
Subject: [Microscopy] TEM: Research Associate position at Leidos Biomedical Research,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The Electron Microscopy Laboratory at the Frederick National Laboratory for Cancer Research, located in Frederick, Maryland, has an open position of Research Associate I. Main duties will include: 1) biological sample preparation for electron microscopy (negative staining, plastic embedding, vitrification for cryo-EM, other approaches as necessary), 2) operation of electron microscopes for imaging and data collection, and 3) analysis and presentation of data. If interested, please apply directly using the following link:

http://jobs.leidos.com/ShowJob/Id/832108/Research-Associate-I-628272-%28NCI%29/

Best regards,

Yaroslav Tsybovsky (Contractor)
Scientist
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc.
Post Office Box B
Frederick, Maryland 21702



==============================Original Headers==============================
7, 47 -- From yaroslav.tsybovsky-at-nih.gov Thu May 12 12:04:23 2016
7, 47 -- Received: from nihxway3out.hub.nih.gov (nihxway3out.hub.nih.gov [128.231.90.111])
7, 47 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CH4NKn001027
7, 47 -- for {Microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:04:23 -0500
7, 47 -- X-SBRS-Extended: Low
7, 47 -- X-IronPortListener: Outbound_SMTP
7, 47 -- IronPort-PHdr: =?us-ascii?q?9a23=3AGVHDpBbZhvUgcMpc3gRB9OH/LSx+4OfEezUN459i?=
7, 47 -- =?us-ascii?q?sYplN5qZpM66bnLW6fgltlLVR4KTs6sC0LqH9fmxEjVYvN6oizMrTt9lb1c9k8?=
7, 47 -- =?us-ascii?q?IYnggtUoauKHbQC7rUVRE8B9lIT1R//nu2YgB/Ecf6YEDO8DXptWZBUiv2OQc9?=
7, 47 -- =?us-ascii?q?HOnpAIma153xjLDjvcSCKFwQ2XKUWvBbElaflU3prM4YgI9veO4a6yDihT92Qd?=
7, 47 -- =?us-ascii?q?lQ3n5iPlmJnhzxtY+a9Z9n9DlM6bp6r5YTGZjge+wEaZAQTHF/ayFmrPHs4FPm?=
7, 47 -- =?us-ascii?q?TACV4WAXVX0Huh9JCBLC9xr9Roa3uSz//KIp/SiRJ8rtRrcsSByn7qxxTwTjjz?=
7, 47 -- =?us-ascii?q?8WcTU+9TeEpNZ3ifcRnAmwrRth2I3FJMmkPeB5ZafUY5taY0thGeB6dm0JMaiJ?=
7, 47 -- =?us-ascii?q?JcNbHuMbOv1cppe7u0AfpxygHgq9LOXuynlHgWGgjv5y6PgoDQyThF9oJNkJqn?=
7, 47 -- =?us-ascii?q?mB6YytOQ=3D=3D?=
7, 47 -- X-IronPort-Anti-Spam-Filtered: true
7, 47 -- X-IronPort-Anti-Spam-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENg?=
7, 47 -- =?us-ascii?q?XYkhXACgTY4FAEBAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTq?=
7, 47 -- =?us-ascii?q?bZIYmiQyDKYIuBZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBA?=
7, 47 -- =?us-ascii?q?Q?=
7, 47 -- X-IPAS-Result: =?us-ascii?q?A2BmAgAetzRX/4JHKJxegzhVfwW5WQENgXYkhXACgTY4FAE?=
7, 47 -- =?us-ascii?q?BAQEBAQEBZBwLgi2CHBIoUQEVFRQFPSYBBBsaiA0FCZwOhTqbZIYmiQyDKYIuB?=
7, 47 -- =?us-ascii?q?ZgnAYV9iCCBaU6EAYMehUECj0EeAQFCgTYMdIE1iCABfgEBAQ?=
7, 47 -- X-IronPort-AV: E=Sophos;i="5.24,610,1454994000";
7, 47 -- d="scan'208";a="459727113"
7, 47 -- Received: from unknown (HELO msgb10.nih.gov) ([156.40.71.130])
7, 47 -- by nihxway3out.hub.nih.gov with ESMTP/TLS/AES256-SHA; 12 May 2016 13:04:02 -0400
7, 47 -- Received: from MSGB01.nih.gov ([169.254.1.154]) by msgb10.nih.gov
7, 47 -- ([169.254.10.4]) with mapi id 14.03.0279.002; Thu, 12 May 2016 13:04:02 -0400
7, 47 -- From: "Tsybovsky, Yaroslav (NIH/NCI) [C]" {yaroslav.tsybovsky-at-nih.gov}
7, 47 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 47 -- Subject: TEM: Research Associate position at Leidos Biomedical Research,
7, 47 -- Inc./Frederick National Laboratory for Cancer Research
7, 47 -- Thread-Topic: TEM: Research Associate position at Leidos Biomedical
7, 47 -- Research, Inc./Frederick National Laboratory for Cancer Research
7, 47 -- Thread-Index: AdGscBobBV8ZFKKkTvmemwETdQ92zg==
7, 47 -- Date: Thu, 12 May 2016 17:04:01 +0000
7, 47 -- Message-ID: {AEB1E864C83965459AE2D8A2139E1B2DD13FE7-at-msgb01.nih.gov}
7, 47 -- Accept-Language: en-US
7, 47 -- Content-Language: en-US
7, 47 -- X-MS-Has-Attach:
7, 47 -- X-MS-TNEF-Correlator:
7, 47 -- x-originating-ip: [156.40.70.6]
7, 47 -- Content-Type: text/plain; charset="us-ascii"
7, 47 -- MIME-Version: 1.0
7, 47 -- Content-Transfer-Encoding: 8bit
7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CH4NKn001027
==============================End of - Headers==============================








From: microscopy.listserver-at-gmail.com
Date: May 12, 2016 at 7:00:52 AM CDT
Subject: [Microscopy] Administrivia: Nestor is doing testing Ignore this message

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Sorry All

Must do a bit of testing to restore functionality which
has broken.

Nestor



--
===============================================

Do not reply to this messages it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

=============================================


==============================Original Headers==============================
10, 46 -- From microscopy.listserver-at-gmail.com Thu May 12 07:00:52 2016
10, 46 -- Received: from mail-io0-f170.google.com (mail-io0-f170.google.com [209.85.223.170])
10, 46 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CC0qbd004807
10, 46 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 07:00:52 -0500
10, 46 -- Received: by mail-io0-f170.google.com with SMTP id f89so91292700ioi.0
10, 46 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 05:01:04 -0700 (PDT)
10, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 46 -- d=gmail.com; s=20120113;
10, 46 -- h=to:from:subject:message-id:date:user-agent:mime-version
10, 46 -- :content-transfer-encoding;
10, 46 -- bh=ekJtcsYBinLLV7t/gEEJVQ5QOJADEcBy3NUO0cVnuZc=;
10, 46 -- b=GyJ+tGPGHDUEsAb0h7Yz0zKz+LqPghxuu95OSYlLw53G1YqC91iaJTcijDL+WgA0Kv
10, 46 -- jMwC4ecsatDV5OtR/HNKnAm2S/jT2svPclT8hqrbT3IoBOQvjKBVEBL7ov5sJnReg5Xq
10, 46 -- 7v3n1wFxYR9lr0HaUuE5VD18JshlfOppsmVKqtiFt2YcPQz517VYrRO69bQsAbK0uVvC
10, 46 -- 1bjNm8d08/ES+Mpk6E5h6gqRwaE+SWvBWjcfgBTV7DZ9HwZjrkXopEKaTAq3NOtWQ8k5
10, 46 -- N4UlcQ5qie2xCqjJIaPAD676u4E1/+gFYebV0hi0BxEfoYilKmV1VagBHKmPEj/MXORg
10, 46 -- C/TQ==
10, 46 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 46 -- d=1e100.net; s=20130820;
10, 46 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent
10, 46 -- :mime-version:content-transfer-encoding;
10, 46 -- bh=ekJtcsYBinLLV7t/gEEJVQ5QOJADEcBy3NUO0cVnuZc=;
10, 46 -- b=CloD5zseNhlgatGNH3lfRaqR3r/eAbyTPJ7noOtcmUFHTo8XQBbkbTWtX6+Ny3lDhM
10, 46 -- 7V4u338tAAPwNytx/SGPqnXUs3PaeO2t52AsP0iz0GcIIKR3cyBI/WxB7gqJNDWPRNFO
10, 46 -- DG05Xf4D2eyfQ5kX57Vs6hMzoeFg2qTD2yoTv+8ujU4V+bQmUFL2C+umcPK3yrMyRu6o
10, 46 -- UcNPwn2/n/sfNgMEEnVmSNJdIRt1oB9EcarlrXco58LBd6CXVizgOYy+Fa9oOtShNigG
10, 46 -- PCkQYN/JHmXieavK4lxgMOay590EQCJSics/ogMht83HhLaHAITIsw54td2YGmr9FkN1
10, 46 -- AXeA==
10, 46 -- X-Gm-Message-State: AOPr4FXwoyUmDhoZaft+gy+96rfJz3fJjkycQABtdDda7wNlWtgzDIzZXklqanZA5CJZfw==
10, 46 -- X-Received: by 10.107.186.6 with SMTP id k6mr7219720iof.136.1463054464304;
10, 46 -- Thu, 12 May 2016 05:01:04 -0700 (PDT)
10, 46 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
10, 46 -- by smtp.googlemail.com with ESMTPSA id qn2sm4458894igc.20.2016.05.12.05.01.03
10, 46 -- for {microscopy-at-microscopy.com}
10, 46 -- (version=TLSv1/SSLv3 cipher=OTHER);
10, 46 -- Thu, 12 May 2016 05:01:03 -0700 (PDT)
10, 46 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
10, 46 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com}
10, 46 -- Subject: Administrivia: Nestor is doing testing Ignore this message
10, 46 -- Message-ID: {40f1ba8c-3a0e-d29e-d491-1218a2688b3e-at-gmail.com}
10, 46 -- Date: Thu, 12 May 2016 07:01:03 -0500
10, 46 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
10, 46 -- Gecko/20100101 Thunderbird/45.0
10, 46 -- MIME-Version: 1.0
10, 46 -- Content-Type: text/plain; charset=utf-8; format=flowed
10, 46 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 12, 2016 at 5:58:02 AM CDT
Subject: [Microscopy] Last announcement - Abstract submission deadline extended to 18th May 2016 43rd SCUR-The SKIN IMAGING SOCIETY-Annual Meeting, LYON,29-30th August 2016

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Note: SCUR Annual meeting will take place during the first 2 days of
EMC16 (the latter will take place Aug, 28 - Sept, 2 in Lyon, France)


Interested in Skin Research - Ultrastructure -Morphology - Imaging techniques and other modern
techniques in Dermatological / Dermatopathological Research & Science?


Dear Colleague(s),

It is still NOT TOO LATE to submit your abstract for presentation at the forthcoming
43rd Annual meeting of SCUR- the Skin Imaging Society, 29th-30th August 2016 in Lyon, France [cf. www.scur.org]

New deadline: May 18th 2016

NOTE: 4 Travel Grants of 200[euro] for junior scientists (students and researchers under 35 y. o.)
and technicians, members of SCUR
(either active members or prospective new members submitting SCUR membership application simultaneously).

THREE Awards
*Best young scientist presentation (200 [euro])
*Best oral presentation (200 [euro])
*Best poster presentation (200 [euro])

Please, send your abstract to { { scur.lyon2016-at-gmail.com } }
(find RTF form on website www.scur.org - and link then to
'Next SCUR Meeting' and find
General information at: https://www.scur.org/content/e1174/e1181/e1182 ,

at the bottom of that page also: all forms
[Announcement = Invitation to Lyon - Society for Cutaneous Ultrastructure Research,
{abstract submission form} , {Social Events Registration Form} ,
{Membership Application Form} as well as the { TRAVEL GRANT APPLICATION FORM} ]

You can visit / attend SCUR sessions if you register for whole EMC-16 - or you register only for SCUR program.
(BUT: 'Registering' also for {SCUR only} must be done via the EMC-16-Website
= http://www.emc2016.fr/en/register/fees - Early Bird Registering until 1 June 2016 -
FIND there (if you only want to attend the SCUR-Meeting) also the rate for:

"2 Days SCUR session only (29th & 30th August) [at a special reduced rate of ] [euro] 250 " )


End your summer vacation with an exciting stop at the { Gaules capital of Lugdunum} :
so many things to see and to do, apart from the scientific sessions, at the heart of the
{Beaujolais region} and the { famous French kitchen} !
(visit: http://www.onlylyon.com/en/ )


SCUR 2016 ALL INFORMATION:


Thanking you for support and further distribution of this message to other interested colleagues/persons.

Looking forward to meeting YOU and many other interested Colleagues there,


With best regards,

Wolfgang MUSS (also Member of MSA)
Secretary of SCUR - The Skin Imaging Society
SALZBURG-AUSTRIA





==============================Original Headers==============================
27, 20 -- From wij.muss-at-aon.at Thu May 12 05:58:01 2016
27, 20 -- Received: from bsmtp2.bon.at (bsmtp2.bon.at [213.33.87.16])
27, 20 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4CAw0SJ015148
27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 05:58:01 -0500
27, 20 -- Received: from MussTHINK (unknown [93.83.25.98])
27, 20 -- by bsmtp2.bon.at (Postfix) with ESMTPSA id 3r592C6kH6z5tlP
27, 20 -- for {microscopy-at-microscopy.com} ; Thu, 12 May 2016 12:58:11 +0200 (CEST)
27, 20 -- From: "MUSS Wolfgang SCUR Secretary" {wij.muss-at-aon.at}
27, 20 -- To: {microscopy-at-microscopy.com}
27, 20 -- Subject: Last announcement - Abstract submission deadline extended to 18th May 2016 43rd SCUR-The SKIN IMAGING SOCIETY-Annual Meeting, LYON,29-30th August 2016
27, 20 -- Date: Thu, 12 May 2016 12:58:14 +0200
27, 20 -- Message-ID: {009101d1ac3d$2f090d80$8d1b2880$-at-aon.at}
27, 20 -- MIME-Version: 1.0
27, 20 -- Content-Type: text/plain;
27, 20 -- charset="UTF-8"
27, 20 -- X-Mailer: Microsoft Outlook 15.0
27, 20 -- Thread-Index: AdGsOXYGBS1uhIMnQHavjW1y8kY0Xw==
27, 20 -- Content-Language: de-at
27, 20 -- Content-Transfer-Encoding: 8bit
27, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4CAw0SJ015148
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 11, 2016 at 7:39:03 PM CDT
Subject: [Microscopy] viaWWW:Job Opening at UCSB: TEM Engineer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: ucsb.materials-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
ucsb.materials-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: ucsb.materials-at-gmail.com Name: Dawn McTague

Organization: University of California, Santa Barbara

Title-Subject: [Filtered] Job Opening at UCSB: TEM Engineer

Message: The University of California Santa Barbara Materials Department has an immediate opening
for a TEM Microscopy Engineer. This position is responsible for autonomously managing the training,
maintenance, operations, and research in transmission electron microscopy (TEM), atom probe
microscopy, and focused ion beam (FIB) technology. This position involves high-level collaborative
research with faculty, students, and research personnel in Materials and other campus units
utilizing the facility. The position also provides training and instruction to students, research
personnel, and outside users in advanced techniques of transmission electron microscopy in addition
to maintenance and upkeep of advanced equipment. Incumbent must have a strong background in
research microscopy with a high level of expertise in TEM microscopy. Experience working in a
fast-paced research environment with a diverse population combined with an ability to develop
thorough training programs is required. Ph.D. preferred.

To view full job posting and/or apply for this position please visit the following URL:

jobs.ucsb.edu/applicants/Central?quickFind=190183

Login Host: 128.111.74.8
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 52 -- From microscopy.listserver-at-gmail.com Wed May 11 19:39:02 2016
14, 52 -- Received: from mail-io0-f175.google.com (mail-io0-f175.google.com [209.85.223.175])
14, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4C0d28j015216
14, 52 -- for {microscopy-at-microscopy.com} ; Wed, 11 May 2016 19:39:02 -0500
14, 52 -- Received: by mail-io0-f175.google.com with SMTP id i75so70756981ioa.3
14, 52 -- for {microscopy-at-microscopy.com} ; Wed, 11 May 2016 17:39:12 -0700 (PDT)
14, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=gmail.com; s=20120113;
14, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=cZdM6paa9nnRu2En7EDFDkv6XZbMlO9qVrQGC72ME0A=;
14, 52 -- b=jE4TbtSwRtjYZlfPaaF+btw7TK8DVFaZEScDDKka5CqP5FDQcyhFkylaf0CQfeU1BJ
14, 52 -- NV5b+KjSsbjhnm7tVcGYyz6YEwvZjYY+FipkTrYvHbVQN/FfPQoveN47ImfQZJiilb6r
14, 52 -- GExQrpR/lUBOn6F5cLLwS8f9t05tedYzLbyEgWjZYoGRMOLioQMgP1P4jpMMdNvDhmH1
14, 52 -- wcz3z7UEJgGO0jshe0OkEnPnRv0ddU8jT3EBTkgfp1DMLbvXKSXIp6LO/D6UQ/BWgVny
14, 52 -- 08bD+mS+ofmzyb/ebfXEwn7XxdeE65+CFhwLZdQJD3+5f568ryV2SWbnw+G/w715cuB3
14, 52 -- R/HQ==
14, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=1e100.net; s=20130820;
14, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=cZdM6paa9nnRu2En7EDFDkv6XZbMlO9qVrQGC72ME0A=;
14, 52 -- b=BUcAaFCpqfikzV6LRFRrtgsxs2AC+ucYkrzBnbdrNfK73JK5SQpPMoyLkX/aERdf4O
14, 52 -- MP7WM9OtqgN4JJdAdwU7V3rfwTJSMIwbslNNs2W5NnXqMiGM/aZr9rSP77MK6FH2d1+L
14, 52 -- S5WhjsuEE7wAG2UpaKmmJw1mLzbGv8wC+XpPz5NqiHLMrn6azj3jPlH6Gc9xzMZh3Dua
14, 52 -- hPo2BHilUQJD/PKU9B4l+EqDs+YLgrM6fDR7voGGMi4fTU+XPXSCieIPD8Ivmhpvccl0
14, 52 -- lckTHrNBdXbAblWb94WKDV/JvwTKMq2L7oAGEZoUiCwkAHGD+tgLmDImT+B67wA9EC2H
14, 52 -- Em3A==
14, 52 -- X-Gm-Message-State: AOPr4FXEL5557AXVCEeE0eqhFhpxA9V3ItYVrGOIylc2B8AOvctYmgL6eeaIzBpni84GMA==
14, 52 -- X-Received: by 10.36.36.4 with SMTP id f4mr4597644ita.29.1463013552584;
14, 52 -- Wed, 11 May 2016 17:39:12 -0700 (PDT)
14, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 52 -- by smtp.googlemail.com with ESMTPSA id d5sm3807915igj.18.2016.05.11.17.39.11
14, 52 -- for {microscopy-at-microscopy.com}
14, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 52 -- Wed, 11 May 2016 17:39:12 -0700 (PDT)
14, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 52 -- {microscopylistserver-noreply-at-microscopy.com}
14, 52 -- Subject: viaWWW:Job Opening at UCSB: TEM Engineer
14, 52 -- References: {201605111514.u4BFEoSK032492-at-ns.microscopy.com}
14, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 52 -- X-Forwarded-Message-Id: {201605111514.u4BFEoSK032492-at-ns.microscopy.com}
14, 52 -- Message-ID: {e33a2ef8-e5f6-30c1-8fc9-f7ae4224aef3-at-microscopy.com}
14, 52 -- Date: Wed, 11 May 2016 19:39:11 -0500
14, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
14, 52 -- Gecko/20100101 Thunderbird/45.0
14, 52 -- MIME-Version: 1.0
14, 52 -- In-Reply-To: {201605111514.u4BFEoSK032492-at-ns.microscopy.com}
14, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 11, 2016 at 6:30:26 AM CDT
Subject: [Microscopy] Workshop announcement: Electron crystallography of 2D crystals of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Sorry, wrong dates: August 22-27, 2016. (Lectures August 23-26).

Henning.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



On 11 May 2016, at 10:49, Henning Stahlberg {henning.stahlberg-at-unibas.ch} wrote:

Dear colleagues,

We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.

Topics include:
Basics of 2D electron crystallography
* Introduction to 2D electron crystallography
* Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
* Cryo-EM data collection of 2D crystals
* Introduction to 2DX
* Drift correction using ZORRO
Basic image processing with 2DX
* Determination of the defocus, crystal tilt geometry, crystal lattice
* Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
* 2D merging and 3D merging with reconstruction via weighted back projection
* Automation of the processing pipeline
Advanced topics in image processing with 2DX
* Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
* Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
* Advanced usage of the 2DX backend library
* Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).

Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at
http://www.2dx.unibas.ch/workshop/2016

For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,

Nikhil Biyani,
Ricardo Righetto,
Robert McLeod,
Henning Stahlberg.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62




==============================Original Headers==============================
9, 41 -- From henning.stahlberg-at-unibas.ch Wed May 11 06:30:24 2016
9, 41 -- Received: from mx2-priv.urz.unibas.ch (mx2-priv.urz.unibas.ch [131.152.226.165])
9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4BBUNAK005765
9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 06:30:23 -0500
9, 41 -- Received: from localhost (localhost [127.0.0.1])
9, 41 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTP id 156422201A8
9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 13:30:31 +0200 (CEST)
9, 41 -- X-Virus-Scanned: amavisd-new at unibas.ch
9, 41 -- Received: from mx2-priv.urz.unibas.ch ([131.152.226.165])
9, 41 -- by localhost (mx2-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024)
9, 41 -- with LMTP id koKeezvvHJfO for {Microscopy-at-microscopy.com} ;
9, 41 -- Wed, 11 May 2016 13:30:31 +0200 (CEST)
9, 41 -- Received: from URZ-HT-CAS-1.urz.unibas.ch (urz-ht-cas-1.urz.unibas.ch [131.152.8.131])
9, 41 -- by mx2-priv.urz.unibas.ch (Postfix) with ESMTPS id 03F652201A2
9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 13:30:31 +0200 (CEST)
9, 41 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by
9, 41 -- URZ-HT-CAS-1.urz.unibas.ch ([fe80::f8bd:7aba:d322:ad34%12]) with mapi id
9, 41 -- 14.03.0279.002; Wed, 11 May 2016 13:30:30 +0200
9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
9, 41 -- To: Microscopy {Microscopy-at-microscopy.com}
9, 41 -- CC: Ricardo Diogo Righetto {ricardo.righetto-at-unibas.ch} ,
9, 41 -- Robert McLeod
9, 41 -- {robert.mcleod-at-unibas.ch} ,
9, 41 -- Nikhil Biyani {nikhil.biyani-at-unibas.ch}
9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of
9, 41 -- membrane proteins with 2DX, August 22-27, 2016
9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals
9, 41 -- of membrane proteins with 2DX, August 22-27, 2016
9, 41 -- Thread-Index: AQHRq3iDqHuL+mRb9Uuzz6r21q4Q2Q==
9, 41 -- Date: Wed, 11 May 2016 11:30:25 +0000
9, 41 -- Message-ID: {AD4DFA52-B645-4E6E-A86B-39E8E9A85A36-at-unibas.ch}
9, 41 -- Accept-Language: en-US, de-CH
9, 41 -- Content-Language: en-US
9, 41 -- X-MS-Has-Attach:
9, 41 -- X-MS-TNEF-Correlator:
9, 41 -- x-originating-ip: [131.152.226.242]
9, 41 -- Content-Type: text/plain; charset="utf-8"
9, 41 -- Content-ID: {4AFC68C688518C448406D6EFD0DEB3B6-at-zuv.ads.unibas.ch}
9, 41 -- MIME-Version: 1.0
9, 41 -- Content-Transfer-Encoding: 8bit
9, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u4BBUNAK005765
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 11, 2016 at 3:49:29 AM CDT
Subject: [Microscopy] Workshop announcement: Electron crystallography of 2D crystals of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We are happy to announce the fifth 2DX Workshop on Electron Crystallography of Membrane Proteins, which will take place at the University of Basel (Switzerland), on August 22-27, 2016. The workshop will provide hands-on training on practical aspects of 2D crystal data collection on a Titan Krios, and cover in depth the theoretical foundations and the practical image processing of 2D crystal cryo-EM images with the 2DX software package. No previous experience is required.

Topics include:
Basics of 2D electron crystallography
* Introduction to 2D electron crystallography
* Cryo-electron microscopy sample preparation for 2D crystals of membrane proteins
* Cryo-EM data collection of 2D crystals
* Introduction to 2DX
* Drift correction using ZORRO
Basic image processing with 2DX
* Determination of the defocus, crystal tilt geometry, crystal lattice
* Crystal Unbending - including movie-mode drift correction of individual unit cells in dose-fractionated image series
* 2D merging and 3D merging with reconstruction via weighted back projection
* Automation of the processing pipeline
Advanced topics in image processing with 2DX
* Approaches for a partial reconstruction of amplitudes and phases inside of the missing cone
* Processing of 2D crystal unit cells with RELION and FREALIGN, under exploitation of neighborhood correlation of certain image parameters
* Advanced usage of the 2DX backend library
* Preparation of final results with quantitative description of statistical values (e.g., resolution) for export to databases (EMDB).

Computing infrastructure will be provided in place and participants are welcome to bring their own data sets and/or computer (OSX or Linux). More information can be found at
http://www.2dx.unibas.ch/workshop/2016

For registration, please include information about your affiliation, and a short motivation statement (max 200 words).
The registration fee is 250 CHF (travel and accommodation is not included), registration deadline is June 30th. As we can only provide a limited number of workstations, we typically accept participants on a "first come - first serve" basis. Please do not hesitate to contact us for any questions. Looking forward to welcoming you in Basel,

Nikhil Biyani,
Ricardo Righetto,
Robert McLeod,
Henning Stahlberg.

Henning Stahlberg, PhD
Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
http://c-cina.org | Tel. +41-61-387 32 62



==============================Original Headers==============================
9, 41 -- From henning.stahlberg-at-unibas.ch Wed May 11 03:49:28 2016
9, 41 -- Received: from mx1-priv.urz.unibas.ch (mx1-priv.urz.unibas.ch [131.152.226.164])
9, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4B8nR35005006
9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 03:49:28 -0500
9, 41 -- Received: from localhost (localhost [127.0.0.1])
9, 41 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTP id 19E063E01F9
9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 10:49:35 +0200 (CEST)
9, 41 -- X-Virus-Scanned: amavisd-new at unibas.ch
9, 41 -- Received: from mx1-priv.urz.unibas.ch ([131.152.226.164])
9, 41 -- by localhost (mx1-mgnt.urz.unibas.ch [127.0.0.1]) (amavisd-new, port 10024)
9, 41 -- with LMTP id U7Dv0uAVLwUf for {Microscopy-at-microscopy.com} ;
9, 41 -- Wed, 11 May 2016 10:49:34 +0200 (CEST)
9, 41 -- Received: from URZ-HT-CAS-4.urz.unibas.ch (urz-ht-cas-4.urz.unibas.ch [131.152.8.134])
9, 41 -- by mx1-priv.urz.unibas.ch (Postfix) with ESMTPS id ABBFC3E0296
9, 41 -- for {Microscopy-at-microscopy.com} ; Wed, 11 May 2016 10:49:25 +0200 (CEST)
9, 41 -- Received: from URZ-MBX-1.urz.unibas.ch ([131.152.8.141]) by
9, 41 -- URZ-HT-CAS-4.urz.unibas.ch ([fe80::5431:4219:b242:4cca%12]) with mapi id
9, 41 -- 14.03.0279.002; Wed, 11 May 2016 10:49:25 +0200
9, 41 -- From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
9, 41 -- To: Microscopy {Microscopy-at-microscopy.com}
9, 41 -- CC: Nikhil Biyani {nikhil.biyani-at-unibas.ch} ,
9, 41 -- Ricardo Diogo Righetto
9, 41 -- {ricardo.righetto-at-unibas.ch} ,
9, 41 -- Robert McLeod {robert.mcleod-at-unibas.ch}
9, 41 -- Subject: Workshop announcement: Electron crystallography of 2D crystals of
9, 41 -- membrane proteins with 2DX, August 17-19.
9, 41 -- Thread-Topic: Workshop announcement: Electron crystallography of 2D crystals
9, 41 -- of membrane proteins with 2DX, August 17-19.
9, 41 -- Thread-Index: AQHRq2IFeqSWzMcRw0iz5Z6nMWDHlw==
9, 41 -- Date: Wed, 11 May 2016 08:49:25 +0000
9, 41 -- Message-ID: {D2C664AF-4F24-4E0D-BF2D-98895C061C72-at-unibas.ch}
9, 41 -- Accept-Language: en-US, de-CH
9, 41 -- Content-Language: en-US
9, 41 -- X-MS-Has-Attach:
9, 41 -- X-MS-TNEF-Correlator:
9, 41 -- x-originating-ip: [131.152.226.242]
9, 41 -- Content-Type: text/plain; charset="utf-8"
9, 41 -- Content-ID: {D08F1F509F7DAA49B3D4132654F1E341-at-zuv.ads.unibas.ch}
9, 41 -- MIME-Version: 1.0
9, 41 -- Content-Transfer-Encoding: 8bit
9, 41 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u4B8nR35005006
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 12:46:34 PM CDT
Subject: [Microscopy] Re: justification of industrial user fee in a university facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

There is a very good summary of many billing scenarios in the NIH FAQ issued in 2013. Well worth a read!

https://grants.nih.gov/grants/guide/notice-files/NOT-OD-13-053.html



Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility


-----Original Message-----
X-from: ajhall-at-prairienanotech.com [mailto:ajhall-at-prairienanotech.com]
Sent: Tuesday, May 10, 2016 1:11 PM
To: Gilpin, Christopher J {gilpin-at-purdue.edu}

Hi Microscopy Listerve and Shouliang Zhang,

This issue has been around for a long time. In general, university equipment is there to support the research efforts of their faculty and students. Commercial analytical labs exist which can serve industrial needs. University labs, though, are often tempted to sell their services to industry to make more money. A valid argument is often raised that this is unfair competition to those commercial labs because the (very
expensive) university equipment most likely has been purchased with federal grants to support academic research. Some feel this means universities should not do industrial work at all, and others feel it's OK so long as they don't undercut the commercial labs, which could open you up to a lawsuit. Some of the big commercial labs are VERY aggressive about this!

Note (for full disclosure): I am with a small commercial service lab but also work in an academic setting.

Allen J. Hall
Prairie Nanotechnology
www.prairienanotech.com

==============================Original Headers==============================
4, 45 -- From ajhall-at-prairienanotech.com Tue May 10 11:50:41 2016 4, 45 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178])
4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AGofMY014855
4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 11:50:41 -0500
4, 45 -- Received: by mail-io0-f178.google.com with SMTP id d62so23241089iof.2
4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:50:47 -0700 (PDT)
4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 45 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623;
4, 45 -- h=message-id:date:from:user-agent:mime-version:to:subject
4, 45 -- :content-transfer-encoding;
4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=;
4, 45 -- b=t2VgeSn9eeNcFDD2LLgMyflPSDj8b8Xq67qC+mzUypN33zLwJmoTXuHN0x+SjFXYAW
4, 45 -- v4X89t2Jd3S35wojKfAdAAhJI8hiVP46ZjhbCVPLOAzTa4wYOtQGwH23UYRxx/TfALN0
4, 45 -- zTrWblxCxB+O9wlMnlGMp6pLXgVzvMwQfopuvVvHWrDPrfve984uxJ07JYv1EhFO/4XT
4, 45 -- 2MpHY5RRS1SsHmtbF4soLrMBk84IS2xbnRPUvgp/ZmXcRQfCm3w6M0bPTuXEv4S+M6eS
4, 45 -- ACAZzAtMOq2YsAmMCTcDvRyx/+OUOwC9BGA02I0on0USdiPya3uHYZcTB+goErwLxQDZ
4, 45 -- 5ySw==
4, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 45 -- d=1e100.net; s=20130820;
4, 45 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to
4, 45 -- :subject:content-transfer-encoding;
4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=;
4, 45 -- b=QFk4kWfMxbDcQtyn1C+gqxGkLC5+SjI0OA/LMHGxfEPxsP3x4V7K4CYXLufcFUVVLM
4, 45 -- c8akf4kBo8MsjTfzOGAgiFZkDsZBSJvogX2nUSazbTA7WyHvx1wqyeGothvxx0SozVg1
4, 45 -- jL3xVRlXGgGtiopZ649700HJWIg4fj2hU9+dXKRjL3ox8x9uJ3nbfOtXJ15rCrNzOfXs
4, 45 -- 1aJ7jZBrMPbIu3hq2RjvzF71eVnFf7xr/F6DJZH8TFn7bhOLCtuZ1Kkp9jVnQqGr9TMH
4, 45 -- 72aIVum6M85oHgNk9CoQGc3TkDYcqnzq+7LGykHyvwy7cTA9K/PSE/Z59z8fPwcwpTDC
4, 45 -- kCpA==
4, 45 -- X-Gm-Message-State: AOPr4FUkQov8olV3AjXGQ3Oz1CyaYwlE6ETR3r6lvbMv7pLDitpTN4n+LhJS933ONrVvDw==
4, 45 -- X-Received: by 10.107.147.138 with SMTP id v132mr41355964iod.38.1462899046675;
4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT)
4, 45 -- Received: from AllenH.local (c-98-212-86-40.hsd1.il.comcast.net. [98.212.86.40])
4, 45 -- by smtp.googlemail.com with ESMTPSA id uy5sm1406989igc.4.2016.05.10.09.50.45
4, 45 -- for {Microscopy-at-microscopy.com}
4, 45 -- (version=TLSv1/SSLv3 cipher=OTHER);
4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT)
4, 45 -- Message-ID: {57321164.6080006-at-prairienanotech.com}
4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500 4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com} 4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105) 4, 45 -- MIME-Version: 1.0 4, 45 -- To: Microscopy-at-microscopy.com 4, 45 -- Subject: Re: justification of industrial user fee in a university facility 4, 45 -- Content-Type: text/plain; charset=windows-1252 4, 45 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 11:50:42 AM CDT
Subject: [Microscopy] Re: justification of industrial user fee in a university facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Microscopy Listerve and Shouliang Zhang,

This issue has been around for a long time. In general, university
equipment is there to support the research efforts of their faculty and
students. Commercial analytical labs exist which can serve industrial
needs. University labs, though, are often tempted to sell their services
to industry to make more money. A valid argument is often raised that
this is unfair competition to those commercial labs because the (very
expensive) university equipment most likely has been purchased with
federal grants to support academic research. Some feel this means
universities should not do industrial work at all, and others feel its
OK so long as they dont undercut the commercial labs, which could open
you up to a lawsuit. Some of the big commercial labs are VERY aggressive
about this!

Note (for full disclosure): I am with a small commercial service lab but
also work in an academic setting.

Allen J. Hall
Prairie Nanotechnology
www.prairienanotech.com

==============================Original Headers==============================
4, 45 -- From ajhall-at-prairienanotech.com Tue May 10 11:50:41 2016
4, 45 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178])
4, 45 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AGofMY014855
4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 11:50:41 -0500
4, 45 -- Received: by mail-io0-f178.google.com with SMTP id d62so23241089iof.2
4, 45 -- for {Microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:50:47 -0700 (PDT)
4, 45 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 45 -- d=prairienanotech-com.20150623.gappssmtp.com; s=20150623;
4, 45 -- h=message-id:date:from:user-agent:mime-version:to:subject
4, 45 -- :content-transfer-encoding;
4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=;
4, 45 -- b=t2VgeSn9eeNcFDD2LLgMyflPSDj8b8Xq67qC+mzUypN33zLwJmoTXuHN0x+SjFXYAW
4, 45 -- v4X89t2Jd3S35wojKfAdAAhJI8hiVP46ZjhbCVPLOAzTa4wYOtQGwH23UYRxx/TfALN0
4, 45 -- zTrWblxCxB+O9wlMnlGMp6pLXgVzvMwQfopuvVvHWrDPrfve984uxJ07JYv1EhFO/4XT
4, 45 -- 2MpHY5RRS1SsHmtbF4soLrMBk84IS2xbnRPUvgp/ZmXcRQfCm3w6M0bPTuXEv4S+M6eS
4, 45 -- ACAZzAtMOq2YsAmMCTcDvRyx/+OUOwC9BGA02I0on0USdiPya3uHYZcTB+goErwLxQDZ
4, 45 -- 5ySw==
4, 45 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 45 -- d=1e100.net; s=20130820;
4, 45 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to
4, 45 -- :subject:content-transfer-encoding;
4, 45 -- bh=Plgywb2O1+ImPmxWFmcid2u5uNXP8Sirx3J2XesJPCs=;
4, 45 -- b=QFk4kWfMxbDcQtyn1C+gqxGkLC5+SjI0OA/LMHGxfEPxsP3x4V7K4CYXLufcFUVVLM
4, 45 -- c8akf4kBo8MsjTfzOGAgiFZkDsZBSJvogX2nUSazbTA7WyHvx1wqyeGothvxx0SozVg1
4, 45 -- jL3xVRlXGgGtiopZ649700HJWIg4fj2hU9+dXKRjL3ox8x9uJ3nbfOtXJ15rCrNzOfXs
4, 45 -- 1aJ7jZBrMPbIu3hq2RjvzF71eVnFf7xr/F6DJZH8TFn7bhOLCtuZ1Kkp9jVnQqGr9TMH
4, 45 -- 72aIVum6M85oHgNk9CoQGc3TkDYcqnzq+7LGykHyvwy7cTA9K/PSE/Z59z8fPwcwpTDC
4, 45 -- kCpA==
4, 45 -- X-Gm-Message-State: AOPr4FUkQov8olV3AjXGQ3Oz1CyaYwlE6ETR3r6lvbMv7pLDitpTN4n+LhJS933ONrVvDw==
4, 45 -- X-Received: by 10.107.147.138 with SMTP id v132mr41355964iod.38.1462899046675;
4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT)
4, 45 -- Received: from AllenH.local (c-98-212-86-40.hsd1.il.comcast.net. [98.212.86.40])
4, 45 -- by smtp.googlemail.com with ESMTPSA id uy5sm1406989igc.4.2016.05.10.09.50.45
4, 45 -- for {Microscopy-at-microscopy.com}
4, 45 -- (version=TLSv1/SSLv3 cipher=OTHER);
4, 45 -- Tue, 10 May 2016 09:50:46 -0700 (PDT)
4, 45 -- Message-ID: {57321164.6080006-at-prairienanotech.com}
4, 45 -- Date: Tue, 10 May 2016 11:50:44 -0500
4, 45 -- From: Allen Hall {ajhall-at-prairienanotech.com}
4, 45 -- User-Agent: Postbox 4.0.8 (Macintosh/20151105)
4, 45 -- MIME-Version: 1.0
4, 45 -- To: Microscopy-at-microscopy.com
4, 45 -- Subject: Re: justification of industrial user fee in a university facility
4, 45 -- Content-Type: text/plain; charset=windows-1252
4, 45 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 9:45:43 AM CDT
Subject: [Microscopy] Re: Cryomicrotomy using glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

Many thanks for all your (so many) answers! The outcome is unambigous
-- nail polish. Now up to me to test.

Kind regards,
Ondrej



On 4 May 2016 1:01 pm, "Ondej Koteck" {ondrej.kotecky-at-gmail.com} wrote:

Dear Listers,

Since years we use diamond knives to cut ultrathin sections of
polymers at low temperature (around -150 *C). When cut the sections
are floating boat/trough integrated on the diamond knife, from where
they are collected.

To cut larger sections we started to make and use glass knives on
which a plastic boat/trough is glued using a "Cavex Set Up Wax", a
dentistry modelling wax, as suggested by the vendor. Unfortunately the
boats fall off at low temperature.

Can anyone suggest a solution or a "glue" that would resist to low temperatures?

Many thanks,
Ondrej


==============================Original Headers==============================
7, 43 -- From ondrej.kotecky-at-gmail.com Tue May 10 09:45:43 2016
7, 43 -- Received: from mail-io0-f182.google.com (mail-io0-f182.google.com [209.85.223.182])
7, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4AEjhPB012444
7, 43 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 09:45:43 -0500
7, 43 -- Received: by mail-io0-f182.google.com with SMTP id d62so18575467iof.2
7, 43 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 07:45:48 -0700 (PDT)
7, 43 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 43 -- d=gmail.com; s=20120113;
7, 43 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to
7, 43 -- :content-transfer-encoding;
7, 43 -- bh=juGi6TFIPSER3WDp1qp0Oi/1SJybMqR1uPUPBQ95RpA=;
7, 43 -- b=NuhwnPcDJx8SuCFQ79A6qP98FEo1b0fr20ciZtWKnmot3xUS8KR1gjGsAl8KjxH2hS
7, 43 -- FNTOyULlsjNktoBCL9oWgOBcpz69WYXolkKC8TRwLBlE3fzl5aPJCQLB7rrWPvDY4uj5
7, 43 -- dzZCpKLFK79fYObe8lrEz3ZESrHxe6TL7jC2RACL3eRwanw+e4y+6e6W4LsiA+1cVrc0
7, 43 -- zAV25AifBQpMvVFGnalzzBZ1RGtVs9cNrRWMB3A6qnwzVUbz6C/NwchNFNfzTZTJInpP
7, 43 -- X4LRWO7c1+2Pw5wDP1it8hvrLs1dT4dlNP7SuIKx8uoV82U/4GLUjyUv/4XZo/rIQmoO
7, 43 -- q7Aw==
7, 43 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
7, 43 -- d=1e100.net; s=20130820;
7, 43 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date
7, 43 -- :message-id:subject:to:content-transfer-encoding;
7, 43 -- bh=juGi6TFIPSER3WDp1qp0Oi/1SJybMqR1uPUPBQ95RpA=;
7, 43 -- b=PWISH5GIMOOqvOEBksuPZ5ybnXbsykEShkZDlgusIIBzPUj7GuEzEeHDWkecPafEGe
7, 43 -- ef1rSSjg+VP2APlUAHQ478F3X+1IeUlO1wEG04ywYDdEm795GlfSmlcrE59zAPEo6x2D
7, 43 -- Ud4Dq3zyfdwfC+zI/g625g5dPSTjCVPyE+xIuqxgsdMM82Cw1IflW+OwJb3BOL3tjRzW
7, 43 -- hJgVF/aZ/Gk56h8Jm4oroZ6Ez5FcRJ7wj2VHNELHRnKdhbyImrmF57Qr1GkSVC4VV2Ng
7, 43 -- JS4YYEtlO6OLPR89mZzQc1fKyEsBCoTsKGJ48cRGpUbqdeIV6b30kLUG2+IZ9JwCExMs
7, 43 -- G02w==
7, 43 -- X-Gm-Message-State: AOPr4FWdqDkr2V2jSjFsXg13Sw4hc/iAyHYTiXtu5CglslkrMZmeOuZPruRxIRKG0fpLPdyu0Qh9v/g1mQ2cZg==
7, 43 -- X-Received: by 10.107.163.75 with SMTP id m72mr40810450ioe.79.1462891548277;
7, 43 -- Tue, 10 May 2016 07:45:48 -0700 (PDT)
7, 43 -- MIME-Version: 1.0
7, 43 -- Received: by 10.107.26.68 with HTTP; Tue, 10 May 2016 07:45:29 -0700 (PDT)
7, 43 -- In-Reply-To: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com}
7, 43 -- References: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com}
7, 43 -- From: =?UTF-8?B?T25kxZllaiBLb3RlY2vDvQ==?= {ondrej.kotecky-at-gmail.com}
7, 43 -- Date: Tue, 10 May 2016 16:45:29 +0200
7, 43 -- Message-ID: {CAJRapMB8sj1Yu1_QHzaAUvBe4xNMujrAudqEim=S6hppyV1_RA-at-mail.gmail.com}
7, 43 -- Subject: Re: Cryomicrotomy using glass knives
7, 43 -- To: microscopy-at-microscopy.com
7, 43 -- Content-Type: text/plain; charset=UTF-8
7, 43 -- Content-Transfer-Encoding: 8bit
7, 43 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u4AEjhPB012444
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 6:32:52 AM CDT
Subject: [Microscopy] viaWWW: JEOL 2010F Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: mjbehr-at-dow.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
mjbehr-at-dow.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: mjbehr-at-dow.com Name: Michael Behr

Organization: The Dow Chemical Company

Title-Subject: [Filtered] JEOL 2010F

Message: Free JEOL 2010F in working condition, with Gatan Enfina, and Bruker XEDS. You pay for
removal from facility in Midland, MI, and shipping to your location. Available June 2016.

Login Host: 216.99.65.88
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
12, 52 -- From microscopy.listserver-at-gmail.com Tue May 10 06:32:51 2016
12, 52 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176])
12, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4ABWpiF031185
12, 52 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 06:32:51 -0500
12, 52 -- Received: by mail-ig0-f176.google.com with SMTP id lr7so7551465igb.1
12, 52 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 04:32:56 -0700 (PDT)
12, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 52 -- d=gmail.com; s=20120113;
12, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
12, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
12, 52 -- bh=OIBEq2ndcEJCWadIgSRXs9AQxODW0OSbYjXY3FIAQTA=;
12, 52 -- b=p79CJptxVHY7wGzm1yEu9ChR5JS9xfB2CHna9nlCnWRv228ONox4sjcUepIsrdS93F
12, 52 -- tfnmVfxHE/ZTpau134fmUcWzdMHl2ZXsiIgySOqUhPItXqfFdZiuO24o45b9XcZgHikx
12, 52 -- p2WE4zRLYmjSHaGI4whnbGKCtmNC3B6ekGXjrgnj4p8WW88/FlHp36tfA8es7DsEzvkM
12, 52 -- a2u3bBogNE09jB0fMsBUHzziK0efpBEa4WZfYQhgHRqNGjMoR+6Wll7+TfNK4EAQxJ/T
12, 52 -- 3tuwPz3wjTKBQi/VsJ6stQ5SrAwnmkBaEgWe0HoZr0tcOQpOh+PEVTaXkPNdkHGiu1wq
12, 52 -- tcZA==
12, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
12, 52 -- d=1e100.net; s=20130820;
12, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
12, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
12, 52 -- bh=OIBEq2ndcEJCWadIgSRXs9AQxODW0OSbYjXY3FIAQTA=;
12, 52 -- b=kWCbriYoVo2leKGzwg8HgggVW1Gg7rhk8WWItSCHL0sFvYPoAkT3vW8pvDU8KP6Fzx
12, 52 -- aEl8JM55Dw+dXO5EQq2kR2pAYQJdGb7SmM5E6YHacXkS8HSMTuZb/xW7lt6kP65mWCOV
12, 52 -- wuwHdiXDS+z1P1CH3Mc7g/qGFanr9l8nywrgq6+viL7vs6He2VUPB9ELe85o/OytkNWp
12, 52 -- c/JQSDFPqCjgtzgaKZiollAz2mOXMXT2JGqnGkSSXFEHrZt7FIL+GtECwVKGenEZkFvA
12, 52 -- r5o2fbjaZwFween38Rd3Hjp6HbJx3hK9WvZCrYHEkka4EIKGZcPxodT59ox9Al1sB85r
12, 52 -- ypRQ==
12, 52 -- X-Gm-Message-State: AOPr4FXMi5Y++GBttzlufBZza3W/vrXn1LsnMGUJJZLjmNOaNiBiN8jnpzNYRm3Uk+Eg9w==
12, 52 -- X-Received: by 10.50.15.131 with SMTP id x3mr16301320igc.18.1462879975560;
12, 52 -- Tue, 10 May 2016 04:32:55 -0700 (PDT)
12, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
12, 52 -- by smtp.googlemail.com with ESMTPSA id 142sm187078itk.12.2016.05.10.04.32.55
12, 52 -- for {microscopy-at-microscopy.com}
12, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
12, 52 -- Tue, 10 May 2016 04:32:55 -0700 (PDT)
12, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
12, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
12, 52 -- {microscopylistserver-noreply-at-microscopy.com}
12, 52 -- Subject: viaWWW: JEOL 2010F Available
12, 52 -- References: {201605091801.u49I1B5X018505-at-ns.microscopy.com}
12, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
12, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
12, 52 -- X-Forwarded-Message-Id: {201605091801.u49I1B5X018505-at-ns.microscopy.com}
12, 52 -- Message-ID: {b1ab9fb7-df96-a708-b06e-34c04d0e6324-at-microscopy.com}
12, 52 -- Date: Tue, 10 May 2016 06:32:54 -0500
12, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
12, 52 -- Gecko/20100101 Thunderbird/45.0
12, 52 -- MIME-Version: 1.0
12, 52 -- In-Reply-To: {201605091801.u49I1B5X018505-at-ns.microscopy.com}
12, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
12, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 10, 2016 at 6:31:23 AM CDT
Subject: [Microscopy] viaWWW:Used 3mm TEM disc puncher, and Tenupol-5 jet

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: fei.long-at-queensu.ca

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
fei.long-at-queensu.ca as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: fei.long-at-queensu.ca Name: Fei Long

Organization: Queen's University

Title-Subject: [Filtered] Used 3mm TEM disc puncher, and Tenupol-5 jet

Message: Hi all,
I am looking to buy a used TEM 3mm disc puncher. Also a set of the jets with 1mm bore for Tenupol-5
electro-polisher, since the ones I have is broken now.

If anyone has these spare or none used parts, I am interested to purchase them.
Thanks,

Fei

Login Host: 74.14.231.44
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 52 -- From microscopy.listserver-at-gmail.com Tue May 10 06:31:23 2016
14, 52 -- Received: from mail-io0-f176.google.com (mail-io0-f176.google.com [209.85.223.176])
14, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u4ABVNhW030512
14, 52 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 06:31:23 -0500
14, 52 -- Received: by mail-io0-f176.google.com with SMTP id 190so12040500iow.1
14, 52 -- for {microscopy-at-microscopy.com} ; Tue, 10 May 2016 04:31:28 -0700 (PDT)
14, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=gmail.com; s=20120113;
14, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=AiTFpS/i170KfvS+o3k6DpA9uCIxrTnvYcl5ASkET+g=;
14, 52 -- b=OawXAqE43sVRPFFvRPF7yvgNaPFEiWsQ1EZs8dY1Nfh0Mnm8REAPLEVML939oh8Kjq
14, 52 -- 2HIvKkQAyVQrsoybE4rAh/uLJpJohJmoEmyfK+SuyFko0FQtNJsxaZH36qAS3MSKH44w
14, 52 -- /iY1afefj68ws0Mo6R59QSfPmporMyOLQq0EnMkiMVvd+UqoVvp4Hx3n09jpoO4onrnQ
14, 52 -- HqyJrvwkIkXnK9bPpAkBfqv9Z/aPpcVhU0hW/ZFGlD+0R0qCQPZsEJgq+vlkoTr0/opM
14, 52 -- 08VanfYQh8Dg09e6r2ijuANdw9atguZznQxxdWge3ADpChS9FYm+ebUjhmvGvhbNP+9M
14, 52 -- Z0Ig==
14, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=1e100.net; s=20130820;
14, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=AiTFpS/i170KfvS+o3k6DpA9uCIxrTnvYcl5ASkET+g=;
14, 52 -- b=Dylla0wKBdyt243ACzMqKoLb4ICZVmNYM6nm/i9BZCRU2Fc6/a+oVUpePvLmbZAjbw
14, 52 -- J6X8BWZBRqnPUFdOhTIj60pCLzHAbfLK/1NxV5cSZfea3DW0ztw73hBj1zEpaiF2FJ5W
14, 52 -- hySmpYS2JHvp9u1RKeLq3fQ1jXFCUmCuFcZJXzS6GpzxPSKD7ATyFDQu4uESeDGRsfGd
14, 52 -- +VHAz0jzZZsjhAR8Na+DiWt6l5laj9CPXekwVMkL2vP1oCMRYhidNA4sbn5Py4BRqFaN
14, 52 -- aMZjPowi7QAOTanE72Q5uSD1Cv4oWwkNnkd6zbWDoqeYSwCSmUS6cW/pESJF1SEXI+ZC
14, 52 -- LwCw==
14, 52 -- X-Gm-Message-State: AOPr4FVTOODJD2cxWzsNYL/8GWqiYOisrcUiSuh04OrOvUCEiIBFblV5q7b7XbOo/wep5Q==
14, 52 -- X-Received: by 10.107.131.12 with SMTP id f12mr43138760iod.126.1462879887683;
14, 52 -- Tue, 10 May 2016 04:31:27 -0700 (PDT)
14, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 52 -- by smtp.googlemail.com with ESMTPSA id v136sm466137ita.3.2016.05.10.04.31.26
14, 52 -- for {microscopy-at-microscopy.com}
14, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 52 -- Tue, 10 May 2016 04:31:27 -0700 (PDT)
14, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 52 -- {microscopylistserver-noreply-at-microscopy.com}
14, 52 -- Subject: viaWWW:Used 3mm TEM disc puncher, and Tenupol-5 jet
14, 52 -- References: {201605091743.u49HhT6j008743-at-ns.microscopy.com}
14, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 52 -- X-Forwarded-Message-Id: {201605091743.u49HhT6j008743-at-ns.microscopy.com}
14, 52 -- Message-ID: {c4193bbd-f5e2-e5e5-0db3-42699244a2f2-at-microscopy.com}
14, 52 -- Date: Tue, 10 May 2016 06:31:26 -0500
14, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
14, 52 -- Gecko/20100101 Thunderbird/45.0
14, 52 -- MIME-Version: 1.0
14, 52 -- In-Reply-To: {201605091743.u49HhT6j008743-at-ns.microscopy.com}
14, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 4:11:00 PM CDT
Subject: [Microscopy] 35 mm camera and controller for Zeiss Axioplan to give away

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,
For any film aficianados out there, I have a
cameraback and mounting bits, as well as the electronic controller, for
a 35 mm camera, that coupled to a Zeiss Axioplan. I was using it up
until around 2002 or so, when it was working fine. It has been kept cool
and out of dust since then. I think it could probably be adapted to any
brand of scope with a bit of hardware tinkering. Free to anyone who
wants it (I am willing to pack it carefully for shipment but not pay
shipping costs). Thanks,
Tobias
University of Massachusetts, Biology Department, 611 N Pleasant St,
Amherst, MA, 01003, USA

==============================Original Headers==============================
1, 21 -- From baskin-at-bio.umass.edu Mon May 9 16:11:00 2016
1, 21 -- Received: from marlin.bio.umass.edu (marlin.bio.umass.edu [128.119.55.19])
1, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49LB0q8023456
1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 16:11:00 -0500
1, 21 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10])
1, 21 -- (authenticated bits=0)
1, 21 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id u49LB2gc013828
1, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO)
1, 21 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 17:11:02 -0400
1, 21 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
1, 21 -- Subject: 35 mm camera and controller for Zeiss Axioplan to give away
1, 21 -- To: microscnet {microscopy-at-microscopy.com}
1, 21 -- Message-ID: {0ff1830d-6227-1e06-a78e-6a1fbc205134-at-bio.umass.edu}
1, 21 -- Date: Mon, 9 May 2016 17:11:02 -0400
1, 21 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
1, 21 -- Gecko/20100101 Thunderbird/45.0
1, 21 -- MIME-Version: 1.0
1, 21 -- Content-Type: text/plain; charset=utf-8; format=flowed
1, 21 -- Content-Transfer-Encoding: 7bit
1, 21 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Mon, 09 May 2016 17:11:02 -0400 (EDT)
1, 21 -- X-Scanned-By: MIMEDefang 2.73
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 1:23:05 PM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Elizabeth

I agree with Michael's comments, these holes are typical for uninfiltrated
dense material. Try letting your specimens sit in uncatalyzed resin at room
temperature a bit longer. For Epon I usually do 3h 25%, over night 50% and
2x2h 100% before going into catalyzed resin at 60C 24h. Leaving out the
catalyst will also improve the viscosity. You can even heat up the
uncatalyzed resin to 37C if you want to push it.
You do have quite some crystal damage from freezing, that together with a
dry acetone FS will extract a considerable amount of material (see my follow
up paper to Walther & Ziegler for more details,
http://www.ncbi.nlm.nih.gov/pubmed/18445157 ). Poorly frozen material
extracts a lot easier. Here are a few suggestions:

1. If you have adherent cells on sapphire discs, lift them out of the
culture dish, touch a filter paper to remove excess medium, dip in
hexedecene, then mount for HPF. This ensures you're only freezing the
minimal layer of medium with your cells. Freezing adherent cells in pure
medium and 200 um deep carriers wil almost certainly fail without
cryoprotection as you're essentially tryin to freeze 200 um of pure water.
(safety tip: repeated skin exposure to hexadecene will give you contact
dermatitis, wear nitrile gloves).
2. If you're working with suspensions, 100 um deep carriers and 20% BSA for
cryoprotection works well with most cells and doesn't mess up your FS (in
contrast to sugars that won't come out easily during FS).
3. FS in acetone, about 1% osmium tetroxide, about 0.1% uranyl acetate,
about 5% water for best contrast. To get there, dissolve OsO4 in acetone,
add 1:20 of your 2% aqueous UA and that's it. Some precipitation is normal
and doesn't influence the end result, it's a saturated solution particularly
at cold temperatures. If it's completely snowed up, try half the amount of
2% UA.
4. I'd advise against prefixation in aldehydes unless your cells are very
sensitive (e.g. primary cells) and you can't get them to the HPF within
30ish minutes.

Good luck!
Chris


-----Original Message-----
X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu]
Sent: Monday, May 9, 2016 10:24 AM
To: cbuser125-at-gmail.com

Hello Elizabeth and Welcome to the Forum, These "holes" look like
un-infiltrated granules. I would see this sometimes with Drosophila eyes
and the pigment granules. Some would be infiltrated and others not. The
fix was to do longer and more infiltration steps
(resin:solvent) and/or switch to a less viscous resin. Were these cells
grown on a sapphire disc or is this a suspension that was HPF/AFS? I know
mast cells have a large number of secretory granules, but not outside the
cells plasma memebrane. Also what is you AFS cocktail? I would also
introduce 5% water to your freezing cocktail for better membrane
preservation, (Walther, P. & Ziegler, A. Freeze substitution of
high-pressure frozen samples: the visibility of biological membranes is
improved when the substitution medium contains water. J. Microsc. 208,
3-10). Also what was your cryo-protectant? (what did you HPF the cells in).
I believe the HPF images should look a lot better, it looks like there is
some freezing artifacts in your cells (look for spider-web like profiles)
and your mitochondria are extracted. Were these cells frozen live? If they
are clinical you can use the HPF-chemical hybrid technique where you lightly
fix the sample in paraformaldehyde only until you can get to the HPF. If
you need a good reference for the HPF/AFS techniques, Mary Morphew at
Colorado (formerly Kent McDonald's lab) has a very detailed manual online:
https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF
experiments should be accompanied by the standard fixation counterpart for
comparison. Perhaps this will give you a clue. Please keep us posted as to
you results.

Sincerely,
Michael Delannoy

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Sent: Monday, May 09, 2016 9:05 AM
To: mdelann1-at-jhmi.edu

X-from: elisabeth.pritz-at-medunigraz.at

This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both
elisabeth.pritz-at-medunigraz.at as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL
cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures
and it would be a great help for me to learn more about ultrastructure of
biological specimen.
We got some unknown structures after high pressure freezing and automated
freeze substitution of mast cell line RBL-2H3 (it is not fully
representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the
bubbles were filled (please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in
a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were
embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we
already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single
bubble one could see electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



https://filesender.aco.net/?vid=71afb1d7-4404-ba68-3ac2-000017427f2b

Login Host: 193.170.104.112
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from the Microscopy Listserver NO-REPLY
forwarding system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
20, 52 -- From microscopy.listserver-at-gmail.com Mon May 9 07:43:52 2016 20,
52 -- Received: from mail-io0-f195.google.com (mail-io0-f195.google.com
[209.85.223.195])
20, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u49Chqg2002158
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 07:43:52
-0500
20, 52 -- Received: by mail-io0-f195.google.com with SMTP id
x35so18657053ioi.0
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 09 May 2016 05:43:53
-0700 (PDT)
20, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=gmail.com; s=20120113;
20, 52 --
h=from:subject:references:to:reply-to:message-id:date:user-agent
20, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 --
b=CxZ+Jjttd5yFDztJ4Hxl4pwL0xyzfdhmlTrmvci7gWYGqtutj2fltbFcuGxDtTxzwn
20, 52 --
1Ju/wKZrZ+5Ru6K+zjJghkdeNep7lUr4wRFivI+BR6V/mXG7gNr+Qkg6KEDlEvF0p02d
20, 52 --
3184YK3jOiUEA/SVRGdO6/SlFj/OsJtFwZa+EG9hzDyb/Xv7sgpV7F8bH3NrpY0xK0c8
20, 52 --
6k0WnEOulbWZ3uvgcXn35Tk2RlnX7CdN/f90rkNhStcACjiWroEzuYntYwBFm4Qz07lj
20, 52 --
MHzvwtGdM126FaR1Ky+d1L+mEANw0Lj2VFdDDn3pxNASz+u1bvLFrpNeyaQqY9ja1Sco
20, 52 -- ajjA==
20, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=1e100.net; s=20130820;
20, 52 --
h=x-gm-message-state:from:subject:references:to:reply-to:message-id
20, 52 --
:date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 --
b=mbqNSjlyOV32mjlpAeKwszk04BRKgJbxbZktA2P6RAGymXVumU9470BzfiVlxYTy9a
20, 52 --
+5IPq1CxtUvPihm+Vm8q1MkBqbdJAjAGKZG7vGJud5zzldKa3/T7jU8DSz0KJp2RDdDD
20, 52 --
pJvk2xx4YyN7oqJPxQRnRontXKvjLN3+uG/TI8JzXLL/sopBrlnecfiAKPXPeXsFIX/E
20, 52 --
XWBH1dNjJa+4maQP/nIIRiRfWcqrpgyasfAIhoYR0XBtm8CxlisJ0wZCW8CyKyE0n4O4
20, 52 --
jCuVQ16vwueP3A6sqK+7DixB8sTxamcI9BP4I3g7B9SHot1z20qiojmQzIVBVkdcgT1B
20, 52 -- dDag==
20, 52 -- X-Gm-Message-State:
AOPr4FXKkZG2rgdlcaWAAfaIVWxB16bB/vjRGTkxuETIDv0QuvEcxTaprt7OJp8Lgb+epg==
20, 52 -- X-Received: by 10.107.7.72 with SMTP id
69mr35779812ioh.64.1462797833451;
20, 52 -- Mon, 09 May 2016 05:43:53 -0700 (PDT)
20, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
20, 52 -- by smtp.googlemail.com with ESMTPSA id
in3sm11224103igb.12.2016.05.09.05.43.52
20, 52 -- for {microscopy-at-microscopy.com}
20, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
20, 52 -- Mon, 09 May 2016 05:43:52 -0700 (PDT)
20, 52 -- From: MicroscopyListserver-NoReply
{microscopy.listserver-at-gmail.com} 20, 52 -- X-Google-Original-From:
MicroscopyListserver-NoReply 20, 52 --
{microscopylistserver-noreply-at-microscopy.com}
20, 52 -- Subject: viaWWW:TEM: Unknown structures after HPF and AFS in RBL
cell line 20, 52 -- References:
{201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20,
52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
20, 52 -- X-Forwarded-Message-Id:
{201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Message-ID: {4685020c-2102-22a8-53f4-c3281806150b-at-microscopy.com}
20, 52 -- Date: Mon, 9 May 2016 07:43:51 -0500 20, 52 -- User-Agent:
Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 20, 52 --
Gecko/20100101 Thunderbird/45.0 20, 52 -- MIME-Version: 1.0 20, 52 --
In-Reply-To: {201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20,
52 -- Content-Transfer-Encoding: 7bit ==============================End of -
Headers==============================


==============================Original Headers==============================
28, 23 -- From prvs=930578cec=mdelann1-at-jhmi.edu Mon May 9 11:59:31 2016
28, 23 -- Received: from smtpauth.johnshopkins.edu
(smtpauth.johnshopkins.edu [128.220.39.139])
28, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u49GxUCL019255
28, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 9 May 2016 11:59:30
-0500
28, 23 -- X-IronPort-AV: E=Sophos;i="5.24,601,1454994000";
28, 23 -- d="scan'208";a="165456481"
28, 23 -- Received: from bsno8925.welch.jhu.edu (HELO BSNO8925)
([10.16.66.96])
28, 23 -- by IronMTW3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 09 May
2016 12:59:32 -0400
28, 23 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu}
28, 23 -- To: {microscopy.listserver-at-gmail.com} ,
{Microscopy-at-microscopy.com} ,
28, 23 -- {microscopy-at-msa.microscopy.com} ,
{elisabeth.pritz-at-medunigraz.at}
28, 23 -- References: {201605091305.u49D5T0D017160-at-ns.microscopy.com}
28, 23 -- In-Reply-To: {201605091305.u49D5T0D017160-at-ns.microscopy.com}
28, 23 -- Subject: RE: [Microscopy] viaWWW:TEM: Unknown structures after HPF
and AFS in RBL cell line
28, 23 -- Date: Mon, 9 May 2016 12:59:33 -0400
28, 23 -- Message-ID: {00d601d1aa14$28fe0920$7afa1b60$-at-jhmi.edu}
28, 23 -- MIME-Version: 1.0
28, 23 -- Content-Type: text/plain;
28, 23 -- charset="us-ascii"
28, 23 -- Content-Transfer-Encoding: 7bit
28, 23 -- X-Mailer: Microsoft Outlook 14.0
28, 23 -- Thread-Index: AQGVlgFfVGb2GCb6xVA6uJc0EyxHR6Ao9GRw
28, 23 -- Content-Language: en-us
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 12:47:47 PM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi
I have very limited experience in TEM so please don't laugh: could they be
air associated or originated from the cell, that formed bubbles during the
procedure?
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.net www.aim.cat
*************************************




-----Original Message-----
X-from: mdelann1-at-jhmi.edu [mailto:mdelann1-at-jhmi.edu]
Sent: Monday, May 9, 2016 8:12 PM
To: eikonika-at-otenet.gr

Hello Elizabeth and Welcome to the Forum, These "holes" look like
un-infiltrated granules. I would see this sometimes with Drosophila eyes
and the pigment granules. Some would be infiltrated and others not. The
fix was to do longer and more infiltration steps
(resin:solvent) and/or switch to a less viscous resin. Were these cells
grown on a sapphire disc or is this a suspension that was HPF/AFS? I know
mast cells have a large number of secretory granules, but not outside the
cells plasma memebrane. Also what is you AFS cocktail? I would also
introduce 5% water to your freezing cocktail for better membrane
preservation, (Walther, P. & Ziegler, A. Freeze substitution of
high-pressure frozen samples: the visibility of biological membranes is
improved when the substitution medium contains water. J. Microsc. 208,
3-10). Also what was your cryo-protectant? (what did you HPF the cells in).
I believe the HPF images should look a lot better, it looks like there is
some freezing artifacts in your cells (look for spider-web like profiles)
and your mitochondria are extracted. Were these cells frozen live? If they
are clinical you can use the HPF-chemical hybrid technique where you lightly
fix the sample in paraformaldehyde only until you can get to the HPF. If
you need a good reference for the HPF/AFS techniques, Mary Morphew at
Colorado (formerly Kent McDonald's lab) has a very detailed manual online:
https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF
experiments should be accompanied by the standard fixation counterpart for
comparison. Perhaps this will give you a clue. Please keep us posted as to
you results.

Sincerely,
Michael Delannoy

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Sent: Monday, May 09, 2016 9:05 AM
To: mdelann1-at-jhmi.edu

X-from: elisabeth.pritz-at-medunigraz.at

This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both
elisabeth.pritz-at-medunigraz.at as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL
cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures
and it would be a great help for me to learn more about ultrastructure of
biological specimen.
We got some unknown structures after high pressure freezing and automated
freeze substitution of mast cell line RBL-2H3 (it is not fully
representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the
bubbles were filled (please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in
a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were
embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we
already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single
bubble one could see electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



https://filesender.aco.net/?vid=71afb1d7-4404-ba68-3ac2-000017427f2b

Login Host: 193.170.104.112
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from the Microscopy Listserver NO-REPLY
forwarding system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
20, 52 -- From microscopy.listserver-at-gmail.com Mon May 9 07:43:52 2016 20,
52 -- Received: from mail-io0-f195.google.com (mail-io0-f195.google.com
[209.85.223.195])
20, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u49Chqg2002158
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 07:43:52
-0500
20, 52 -- Received: by mail-io0-f195.google.com with SMTP id
x35so18657053ioi.0
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 09 May 2016 05:43:53
-0700 (PDT)
20, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=gmail.com; s=20120113;
20, 52 --
h=from:subject:references:to:reply-to:message-id:date:user-agent
20, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 --
b=CxZ+Jjttd5yFDztJ4Hxl4pwL0xyzfdhmlTrmvci7gWYGqtutj2fltbFcuGxDtTxzwn
20, 52 --
1Ju/wKZrZ+5Ru6K+zjJghkdeNep7lUr4wRFivI+BR6V/mXG7gNr+Qkg6KEDlEvF0p02d
20, 52 --
3184YK3jOiUEA/SVRGdO6/SlFj/OsJtFwZa+EG9hzDyb/Xv7sgpV7F8bH3NrpY0xK0c8
20, 52 --
6k0WnEOulbWZ3uvgcXn35Tk2RlnX7CdN/f90rkNhStcACjiWroEzuYntYwBFm4Qz07lj
20, 52 --
MHzvwtGdM126FaR1Ky+d1L+mEANw0Lj2VFdDDn3pxNASz+u1bvLFrpNeyaQqY9ja1Sco
20, 52 -- ajjA==
20, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=1e100.net; s=20130820;
20, 52 --
h=x-gm-message-state:from:subject:references:to:reply-to:message-id
20, 52 --
:date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 --
b=mbqNSjlyOV32mjlpAeKwszk04BRKgJbxbZktA2P6RAGymXVumU9470BzfiVlxYTy9a
20, 52 --
+5IPq1CxtUvPihm+Vm8q1MkBqbdJAjAGKZG7vGJud5zzldKa3/T7jU8DSz0KJp2RDdDD
20, 52 --
pJvk2xx4YyN7oqJPxQRnRontXKvjLN3+uG/TI8JzXLL/sopBrlnecfiAKPXPeXsFIX/E
20, 52 --
XWBH1dNjJa+4maQP/nIIRiRfWcqrpgyasfAIhoYR0XBtm8CxlisJ0wZCW8CyKyE0n4O4
20, 52 --
jCuVQ16vwueP3A6sqK+7DixB8sTxamcI9BP4I3g7B9SHot1z20qiojmQzIVBVkdcgT1B
20, 52 -- dDag==
20, 52 -- X-Gm-Message-State:
AOPr4FXKkZG2rgdlcaWAAfaIVWxB16bB/vjRGTkxuETIDv0QuvEcxTaprt7OJp8Lgb+epg==
20, 52 -- X-Received: by 10.107.7.72 with SMTP id
69mr35779812ioh.64.1462797833451;
20, 52 -- Mon, 09 May 2016 05:43:53 -0700 (PDT)
20, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
20, 52 -- by smtp.googlemail.com with ESMTPSA id
in3sm11224103igb.12.2016.05.09.05.43.52
20, 52 -- for {microscopy-at-microscopy.com}
20, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
20, 52 -- Mon, 09 May 2016 05:43:52 -0700 (PDT)
20, 52 -- From: MicroscopyListserver-NoReply
{microscopy.listserver-at-gmail.com} 20, 52 -- X-Google-Original-From:
MicroscopyListserver-NoReply 20, 52 --
{microscopylistserver-noreply-at-microscopy.com}
20, 52 -- Subject: viaWWW:TEM: Unknown structures after HPF and AFS in RBL
cell line 20, 52 -- References:
{201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20,
52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
20, 52 -- X-Forwarded-Message-Id:
{201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Message-ID: {4685020c-2102-22a8-53f4-c3281806150b-at-microscopy.com}
20, 52 -- Date: Mon, 9 May 2016 07:43:51 -0500 20, 52 -- User-Agent:
Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 20, 52 --
Gecko/20100101 Thunderbird/45.0 20, 52 -- MIME-Version: 1.0 20, 52 --
In-Reply-To: {201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20,
52 -- Content-Transfer-Encoding: 7bit ==============================End of -
Headers==============================


==============================Original Headers==============================
28, 23 -- From prvs=930578cec=mdelann1-at-jhmi.edu Mon May 9 11:59:31 2016
28, 23 -- Received: from smtpauth.johnshopkins.edu
(smtpauth.johnshopkins.edu [128.220.39.139])
28, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u49GxUCL019255
28, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 9 May 2016 11:59:30
-0500
28, 23 -- X-IronPort-AV: E=Sophos;i="5.24,601,1454994000";
28, 23 -- d="scan'208";a="165456481"
28, 23 -- Received: from bsno8925.welch.jhu.edu (HELO BSNO8925)
([10.16.66.96])
28, 23 -- by IronMTW3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 09 May
2016 12:59:32 -0400
28, 23 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu}
28, 23 -- To: {microscopy.listserver-at-gmail.com} ,
{Microscopy-at-microscopy.com} ,
28, 23 -- {microscopy-at-msa.microscopy.com} ,
{elisabeth.pritz-at-medunigraz.at}
28, 23 -- References: {201605091305.u49D5T0D017160-at-ns.microscopy.com}
28, 23 -- In-Reply-To: {201605091305.u49D5T0D017160-at-ns.microscopy.com}
28, 23 -- Subject: RE: [Microscopy] viaWWW:TEM: Unknown structures after HPF
and AFS in RBL cell line
28, 23 -- Date: Mon, 9 May 2016 12:59:33 -0400
28, 23 -- Message-ID: {00d601d1aa14$28fe0920$7afa1b60$-at-jhmi.edu}
28, 23 -- MIME-Version: 1.0
28, 23 -- Content-Type: text/plain;
28, 23 -- charset="us-ascii"
28, 23 -- Content-Transfer-Encoding: 7bit
28, 23 -- X-Mailer: Microsoft Outlook 14.0
28, 23 -- Thread-Index: AQGVlgFfVGb2GCb6xVA6uJc0EyxHR6Ao9GRw
28, 23 -- Content-Language: en-us
40, 23 -- From eikonika-at-otenet.gr Mon May 9 12:47:46 2016
40, 23 -- Received: from echidna.otenet.gr (smtp-out33.otenet.gr [83.235.69.33])
40, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49HljhC008847
40, 23 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 12:47:46 -0500
40, 23 -- Received: from VAIO (ppp-2-84-165-109.home.otenet.gr [2.84.165.109])
40, 23 -- by echidna.otenet.gr (ESMTP) with ESMTPSA;
40, 23 -- Mon, 9 May 2016 20:47:44 +0300 (EEST)
40, 23 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr}
40, 23 -- To: {mdelann1-at-jhmi.edu}
40, 23 -- Cc: {microscopy-at-microscopy.com}
40, 23 -- References: {201605091711.u49HBtTw026456-at-ns.microscopy.com}
40, 23 -- In-Reply-To: {201605091711.u49HBtTw026456-at-ns.microscopy.com}
40, 23 -- Subject: RE: [Microscopy] RE: viaWWW:TEM: Unknown structures after HPF and AFS in RBL cell line
40, 23 -- Date: Mon, 9 May 2016 20:47:45 +0300
40, 23 -- Message-ID: {000001d1aa1a$e5976170$b0c62450$-at-otenet.gr}
40, 23 -- MIME-Version: 1.0
40, 23 -- Content-Type: text/plain;
40, 23 -- charset="iso-8859-1"
40, 23 -- X-Mailer: Microsoft Outlook 14.0
40, 23 -- Thread-Index: AQF2lXjEJd2JaPmhhIjWxF8a5TDPsaBnCPZA
40, 23 -- Content-Language: en-us
40, 23 -- Content-Transfer-Encoding: 8bit
40, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u49HljhC008847
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 11:59:31 AM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Elizabeth and Welcome to the Forum,
These "holes" look like un-infiltrated granules. I would see this sometimes
with Drosophila eyes and the pigment granules. Some would be infiltrated
and others not. The fix was to do longer and more infiltration steps
(resin:solvent) and/or switch to a less viscous resin. Were these cells
grown on a sapphire disc or is this a suspension that was HPF/AFS? I know
mast cells have a large number of secretory granules, but not outside the
cells plasma memebrane. Also what is you AFS cocktail? I would also
introduce 5% water to your freezing cocktail for better membrane
preservation, (Walther, P. & Ziegler, A. Freeze substitution of
high-pressure frozen samples: the visibility of biological membranes is
improved when the substitution medium contains water. J. Microsc. 208,
3-10). Also what was your cryo-protectant? (what did you HPF the cells in).
I believe the HPF images should look a lot better, it looks like there is
some freezing artifacts in your cells (look for spider-web like profiles)
and your mitochondria are extracted. Were these cells frozen live? If they
are clinical you can use the HPF-chemical hybrid technique where you lightly
fix the sample in paraformaldehyde only until you can get to the HPF. If
you need a good reference for the HPF/AFS techniques, Mary Morphew at
Colorado (formerly Kent McDonald's lab) has a very detailed manual online:
https://mcdb.colorado.edu/facilities/ems/pdf/mmanual.pdf. Finally all HPF
experiments should be accompanied by the standard fixation counterpart for
comparison. Perhaps this will give you a clue. Please keep us posted as to
you results.

Sincerely,
Michael Delannoy

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Sent: Monday, May 09, 2016 9:05 AM
To: mdelann1-at-jhmi.edu

X-from: elisabeth.pritz-at-medunigraz.at

This Question/Comment was submitted to the Microscopy Listserver using the
WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both
elisabeth.pritz-at-medunigraz.at as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL
cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures
and it would be a great help for me to learn more about ultrastructure of
biological specimen.
We got some unknown structures after high pressure freezing and automated
freeze substitution of mast cell line RBL-2H3 (it is not fully
representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the
bubbles were filled (please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in
a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were
embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we
already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single
bubble one could see electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



https://filesender.aco.net/?vid=71afb1d7-4404-ba68-3ac2-000017427f2b

Login Host: 193.170.104.112
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from the Microscopy Listserver NO-REPLY
forwarding system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
20, 52 -- From microscopy.listserver-at-gmail.com Mon May 9 07:43:52 2016 20,
52 -- Received: from mail-io0-f195.google.com (mail-io0-f195.google.com
[209.85.223.195])
20, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id
u49Chqg2002158
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 07:43:52
-0500
20, 52 -- Received: by mail-io0-f195.google.com with SMTP id
x35so18657053ioi.0
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 09 May 2016 05:43:53
-0700 (PDT)
20, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=gmail.com; s=20120113;
20, 52 --
h=from:subject:references:to:reply-to:message-id:date:user-agent
20, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 --
b=CxZ+Jjttd5yFDztJ4Hxl4pwL0xyzfdhmlTrmvci7gWYGqtutj2fltbFcuGxDtTxzwn
20, 52 --
1Ju/wKZrZ+5Ru6K+zjJghkdeNep7lUr4wRFivI+BR6V/mXG7gNr+Qkg6KEDlEvF0p02d
20, 52 --
3184YK3jOiUEA/SVRGdO6/SlFj/OsJtFwZa+EG9hzDyb/Xv7sgpV7F8bH3NrpY0xK0c8
20, 52 --
6k0WnEOulbWZ3uvgcXn35Tk2RlnX7CdN/f90rkNhStcACjiWroEzuYntYwBFm4Qz07lj
20, 52 --
MHzvwtGdM126FaR1Ky+d1L+mEANw0Lj2VFdDDn3pxNASz+u1bvLFrpNeyaQqY9ja1Sco
20, 52 -- ajjA==
20, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=1e100.net; s=20130820;
20, 52 --
h=x-gm-message-state:from:subject:references:to:reply-to:message-id
20, 52 --
:date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 --
b=mbqNSjlyOV32mjlpAeKwszk04BRKgJbxbZktA2P6RAGymXVumU9470BzfiVlxYTy9a
20, 52 --
+5IPq1CxtUvPihm+Vm8q1MkBqbdJAjAGKZG7vGJud5zzldKa3/T7jU8DSz0KJp2RDdDD
20, 52 --
pJvk2xx4YyN7oqJPxQRnRontXKvjLN3+uG/TI8JzXLL/sopBrlnecfiAKPXPeXsFIX/E
20, 52 --
XWBH1dNjJa+4maQP/nIIRiRfWcqrpgyasfAIhoYR0XBtm8CxlisJ0wZCW8CyKyE0n4O4
20, 52 --
jCuVQ16vwueP3A6sqK+7DixB8sTxamcI9BP4I3g7B9SHot1z20qiojmQzIVBVkdcgT1B
20, 52 -- dDag==
20, 52 -- X-Gm-Message-State:
AOPr4FXKkZG2rgdlcaWAAfaIVWxB16bB/vjRGTkxuETIDv0QuvEcxTaprt7OJp8Lgb+epg==
20, 52 -- X-Received: by 10.107.7.72 with SMTP id
69mr35779812ioh.64.1462797833451;
20, 52 -- Mon, 09 May 2016 05:43:53 -0700 (PDT)
20, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
20, 52 -- by smtp.googlemail.com with ESMTPSA id
in3sm11224103igb.12.2016.05.09.05.43.52
20, 52 -- for {microscopy-at-microscopy.com}
20, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
20, 52 -- Mon, 09 May 2016 05:43:52 -0700 (PDT)
20, 52 -- From: MicroscopyListserver-NoReply
{microscopy.listserver-at-gmail.com} 20, 52 -- X-Google-Original-From:
MicroscopyListserver-NoReply 20, 52 --
{microscopylistserver-noreply-at-microscopy.com}
20, 52 -- Subject: viaWWW:TEM: Unknown structures after HPF and AFS in RBL
cell line 20, 52 -- References:
{201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 20,
52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
20, 52 -- X-Forwarded-Message-Id:
{201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Message-ID: {4685020c-2102-22a8-53f4-c3281806150b-at-microscopy.com}
20, 52 -- Date: Mon, 9 May 2016 07:43:51 -0500 20, 52 -- User-Agent:
Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0) 20, 52 --
Gecko/20100101 Thunderbird/45.0 20, 52 -- MIME-Version: 1.0 20, 52 --
In-Reply-To: {201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed 20,
52 -- Content-Transfer-Encoding: 7bit ==============================End of -
Headers==============================


==============================Original Headers==============================
28, 23 -- From prvs=930578cec=mdelann1-at-jhmi.edu Mon May 9 11:59:31 2016
28, 23 -- Received: from smtpauth.johnshopkins.edu (smtpauth.johnshopkins.edu [128.220.39.139])
28, 23 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49GxUCL019255
28, 23 -- for {Microscopy-at-microscopy.com} ; Mon, 9 May 2016 11:59:30 -0500
28, 23 -- X-IronPort-AV: E=Sophos;i="5.24,601,1454994000";
28, 23 -- d="scan'208";a="165456481"
28, 23 -- Received: from bsno8925.welch.jhu.edu (HELO BSNO8925) ([10.16.66.96])
28, 23 -- by IronMTW3.johnshopkins.edu with ESMTP/TLS/AES256-SHA; 09 May 2016 12:59:32 -0400
28, 23 -- From: "Michael Delannoy" {mdelann1-at-jhmi.edu}
28, 23 -- To: {microscopy.listserver-at-gmail.com} , {Microscopy-at-microscopy.com} ,
28, 23 -- {microscopy-at-msa.microscopy.com} , {elisabeth.pritz-at-medunigraz.at}
28, 23 -- References: {201605091305.u49D5T0D017160-at-ns.microscopy.com}
28, 23 -- In-Reply-To: {201605091305.u49D5T0D017160-at-ns.microscopy.com}
28, 23 -- Subject: RE: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in RBL cell line
28, 23 -- Date: Mon, 9 May 2016 12:59:33 -0400
28, 23 -- Message-ID: {00d601d1aa14$28fe0920$7afa1b60$-at-jhmi.edu}
28, 23 -- MIME-Version: 1.0
28, 23 -- Content-Type: text/plain;
28, 23 -- charset="us-ascii"
28, 23 -- Content-Transfer-Encoding: 7bit
28, 23 -- X-Mailer: Microsoft Outlook 14.0
28, 23 -- Thread-Index: AQGVlgFfVGb2GCb6xVA6uJc0EyxHR6Ao9GRw
28, 23 -- Content-Language: en-us
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 8:32:01 AM CDT
Subject: [Microscopy] New photo printer for micrographs, for those interested

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

To recap: I placed a request for opinions on photo printers for
micrographs, now that HP seems to be out of the game.
I got a few responses (surprisingly few, given past interest in this
topic), but those that expressed a preference all liked the Canon P600.
Which does look good on paper (it's Monday morning, so that's the best
pun I've got).

Thanks to all who responded.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
3, 30 -- From oshel1pe-at-cmich.edu Mon May 9 08:32:00 2016
3, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
3, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49DW0KG025044
3, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 08:32:00 -0500
3, 30 -- Received: from cas.cmich.edu ([141.209.15.90])
3, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u49DVxZv025935
3, 30 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 09:32:03 -0400
3, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by
3, 30 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server
3, 30 -- (TLS) id 14.3.248.2; Mon, 9 May 2016 09:32:00 -0400
3, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu
3, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Mon, 9 May
3, 30 -- 2016 09:32:00 -0400
3, 30 -- Message-ID: {57309150.4030202-at-cmich.edu}
3, 30 -- Date: Mon, 9 May 2016 09:32:00 -0400
3, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 30 -- Reply-To: {oshel1pe-at-cmich.edu}
3, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
3, 30 -- MIME-Version: 1.0
3, 30 -- To: micro {microscopy-at-microscopy.com}
3, 30 -- Subject: New photo printer for micrographs, for those interested
3, 30 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
3, 30 -- Content-Transfer-Encoding: 7bit
3, 30 -- X-Originating-IP: [141.209.2.100]
3, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
3, 30 -- X-Spam-Score: -0.30 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,SPF(softfail:0),Bayes(0.0001:-0.5)
3, 30 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
3, 30 -- X-CanItPRO-Stream: default
3, 30 -- X-Canit-Stats-ID: 02QQdw3IV - 77565d9be353 - 20160509
3, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 9, 2016 at 7:43:53 AM CDT
Subject: [Microscopy] viaWWW:TEM: Unknown structures after HPF and AFS in RBL cell line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: elisabeth.pritz-at-medunigraz.at

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
elisabeth.pritz-at-medunigraz.at as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: elisabeth.pritz-at-medunigraz.at Name: Elisabeth Pritz

Organization: Medical Univeristiy Graz

Title-Subject: [Filtered] TEM: Unknown structures after HPF and AFS in RBL cell line

Message: Hello,

it's my first time using this forum - maybe someone knows these structures and it would be a great
help for me to learn more about ultrastructure of biological specimen.
We got some unknown structures after high pressure freezing and automated freeze substitution of
mast cell line RBL-2H3 (it is not fully representative for mast cell line).

There is a ring of "bubbles" around each cell. However, it seems that the bubbles were filled
(please use link below for downloading the pictures).
As mentioned before we did high pressure freezing and freeze substitution in a mixture of acetone
(waterfree) with osmium tetroxide and uranylacetate. The samples were embedded in TAAB epoxy resin.
Unfortunately the membrane contrast is very poor in these samples, though we already fixed that problem.
These "bubbles" appear only close to the cells and in nearly every single bubble one could see
electron dense remnants.

Is it possible, that the structures are a kind of degranulated vesicles?

Thanks for your support!

BR
Elisabeth



https://filesender.aco.net/?vid=71afb1d7-4404-ba68-3ac2-000017427f2b

Login Host: 193.170.104.112
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
20, 52 -- From microscopy.listserver-at-gmail.com Mon May 9 07:43:52 2016
20, 52 -- Received: from mail-io0-f195.google.com (mail-io0-f195.google.com [209.85.223.195])
20, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u49Chqg2002158
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 9 May 2016 07:43:52 -0500
20, 52 -- Received: by mail-io0-f195.google.com with SMTP id x35so18657053ioi.0
20, 52 -- for {microscopy-at-microscopy.com} ; Mon, 09 May 2016 05:43:53 -0700 (PDT)
20, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=gmail.com; s=20120113;
20, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
20, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 -- b=CxZ+Jjttd5yFDztJ4Hxl4pwL0xyzfdhmlTrmvci7gWYGqtutj2fltbFcuGxDtTxzwn
20, 52 -- 1Ju/wKZrZ+5Ru6K+zjJghkdeNep7lUr4wRFivI+BR6V/mXG7gNr+Qkg6KEDlEvF0p02d
20, 52 -- 3184YK3jOiUEA/SVRGdO6/SlFj/OsJtFwZa+EG9hzDyb/Xv7sgpV7F8bH3NrpY0xK0c8
20, 52 -- 6k0WnEOulbWZ3uvgcXn35Tk2RlnX7CdN/f90rkNhStcACjiWroEzuYntYwBFm4Qz07lj
20, 52 -- MHzvwtGdM126FaR1Ky+d1L+mEANw0Lj2VFdDDn3pxNASz+u1bvLFrpNeyaQqY9ja1Sco
20, 52 -- ajjA==
20, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
20, 52 -- d=1e100.net; s=20130820;
20, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
20, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
20, 52 -- bh=obmkF+vmlsDAvMovJnsvUQj+zhk/qCoKY/8pA/z2OYY=;
20, 52 -- b=mbqNSjlyOV32mjlpAeKwszk04BRKgJbxbZktA2P6RAGymXVumU9470BzfiVlxYTy9a
20, 52 -- +5IPq1CxtUvPihm+Vm8q1MkBqbdJAjAGKZG7vGJud5zzldKa3/T7jU8DSz0KJp2RDdDD
20, 52 -- pJvk2xx4YyN7oqJPxQRnRontXKvjLN3+uG/TI8JzXLL/sopBrlnecfiAKPXPeXsFIX/E
20, 52 -- XWBH1dNjJa+4maQP/nIIRiRfWcqrpgyasfAIhoYR0XBtm8CxlisJ0wZCW8CyKyE0n4O4
20, 52 -- jCuVQ16vwueP3A6sqK+7DixB8sTxamcI9BP4I3g7B9SHot1z20qiojmQzIVBVkdcgT1B
20, 52 -- dDag==
20, 52 -- X-Gm-Message-State: AOPr4FXKkZG2rgdlcaWAAfaIVWxB16bB/vjRGTkxuETIDv0QuvEcxTaprt7OJp8Lgb+epg==
20, 52 -- X-Received: by 10.107.7.72 with SMTP id 69mr35779812ioh.64.1462797833451;
20, 52 -- Mon, 09 May 2016 05:43:53 -0700 (PDT)
20, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
20, 52 -- by smtp.googlemail.com with ESMTPSA id in3sm11224103igb.12.2016.05.09.05.43.52
20, 52 -- for {microscopy-at-microscopy.com}
20, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
20, 52 -- Mon, 09 May 2016 05:43:52 -0700 (PDT)
20, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
20, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
20, 52 -- {microscopylistserver-noreply-at-microscopy.com}
20, 52 -- Subject: viaWWW:TEM: Unknown structures after HPF and AFS in RBL cell line
20, 52 -- References: {201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
20, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
20, 52 -- X-Forwarded-Message-Id: {201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Message-ID: {4685020c-2102-22a8-53f4-c3281806150b-at-microscopy.com}
20, 52 -- Date: Mon, 9 May 2016 07:43:51 -0500
20, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
20, 52 -- Gecko/20100101 Thunderbird/45.0
20, 52 -- MIME-Version: 1.0
20, 52 -- In-Reply-To: {201605090843.u498hASb022331-at-ns.microscopy.com}
20, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
20, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 8, 2016 at 2:19:42 PM CDT
Subject: [Microscopy] viaWWW:detailed instructions for LEO (Zeiss) 1450VP SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: scottserata-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
scottserata-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: scottserata-at-gmail.com Name: Scott Serata

Organization: California Academy of Sciences/JEOL

Title-Subject: [Filtered] detailed instructions for LEO (Zeiss) 1450VP SEM

Message: Several people have asked me to post this on the listserve. I have written detailed
instructions for the LEO/Zeiss 1450VP SEM. I was the engineer at the California Academy of Sciences
responsible for training users and fixing this machine for 15 years, until I finally got rid of it
last year. (I now work for JEOL) Our machine did not have any analytical equipment (X-ray EDS/WDS)
on it, just the SE, VPSE and BSD detectors. If you have a LEO 1450 VP SEM this maybe useful. It is
in PDF format and about 13 MB.

Scott Serata

click on this link to get it from my Google drive
https://drive.google.com/open?id=0B2-Pe5FzqYv2TWdUNXNLQTZLQmM

Login Host: 50.206.76.226
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 52 -- From microscopy.listserver-at-gmail.com Sun May 8 14:19:41 2016
14, 52 -- Received: from mail-ig0-f176.google.com (mail-ig0-f176.google.com [209.85.213.176])
14, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u48JJf8X017664
14, 52 -- for {microscopy-at-microscopy.com} ; Sun, 8 May 2016 14:19:41 -0500
14, 52 -- Received: by mail-ig0-f176.google.com with SMTP id u10so88084739igr.1
14, 52 -- for {microscopy-at-microscopy.com} ; Sun, 08 May 2016 12:22:44 -0700 (PDT)
14, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=gmail.com; s=20120113;
14, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=iedXek+dy7iCgGEauynsypHSF6rJfiLyBIOwp9efiXk=;
14, 52 -- b=hSnbYzZNJ+94dfbM4iA8MV97HgPDxJtlUN4HZD/bI7mVMNGFczg8VLye1uIoB/aw/S
14, 52 -- PBV9mMX3DFKlqeiFzzSzsq4pJQUcW33qmbaibS8RXECqUc0RjHPmszB8jzuobeHN/9VQ
14, 52 -- 9JFH0HOYf8WpjtUUdOcUuIBSlBkvg2Kkv73X34d1RCjRw+sRFxFExRZV1B7PT3lMNb/B
14, 52 -- 5j0iJafv4gbIhXtpt3mHAdnmOGXZw0NJU7BdwJ8ebJumSCDv4Gvkjdns1tFcD4uDje09
14, 52 -- UaF22AsIdgFxI7eeLsw8Chkdao+s65xm3xbOpsqKxdS6jM5Toaz2tyWE4xr9On8QeItL
14, 52 -- wk4Q==
14, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=1e100.net; s=20130820;
14, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=iedXek+dy7iCgGEauynsypHSF6rJfiLyBIOwp9efiXk=;
14, 52 -- b=lAgnIeGoO1VTP1a9UwoXmpcWSG7JzZD/mGgZhCLwQcxR/Lv5jLUVa3kUTfpfdHgxVU
14, 52 -- 2GyRim1ti1pB9UmufUr7gn1Qkbz8hV/zJnetSqWCbNRiH8K+OW04Fed3uwvgOMzaYMGd
14, 52 -- CqnaBr4OioUoPLyoYI9F9h9PPrxUMCxRrrnkbkApFdpdsmGXgEVmxP4aWmTKFsarMOtQ
14, 52 -- Hk73dna08F2CCvVISauOPet27d8T9mLShBbKvaPv2QGVGP/5GT+BPJRSK9TmgSwTPX/f
14, 52 -- jA7b38C2Vd/Rrqh92Rh9W6QexM+z8ZKtw3rrin3zn5Bj7PKxnQsVvpRRdTpCrXYTynqm
14, 52 -- wUlw==
14, 52 -- X-Gm-Message-State: AOPr4FUt8sMCG2z1nZvy8LECqYiL23pDuaY09bGIlcHVqtRJdWRhTtFdpF9nIPjCF6osrw==
14, 52 -- X-Received: by 10.50.28.113 with SMTP id a17mr7167401igh.44.1462735363855;
14, 52 -- Sun, 08 May 2016 12:22:43 -0700 (PDT)
14, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 52 -- by smtp.googlemail.com with ESMTPSA id f14sm1725639ioi.2.2016.05.08.12.22.43
14, 52 -- for {microscopy-at-microscopy.com}
14, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 52 -- Sun, 08 May 2016 12:22:43 -0700 (PDT)
14, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 52 -- {microscopylistserver-noreply-at-microscopy.com}
14, 52 -- Subject: viaWWW:detailed instructions for LEO (Zeiss) 1450VP SEM
14, 52 -- References: {201605081644.u48GiGY8011428-at-ns.microscopy.com}
14, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 52 -- X-Forwarded-Message-Id: {201605081644.u48GiGY8011428-at-ns.microscopy.com}
14, 52 -- Message-ID: {ddfb012b-f29d-0b7f-33fb-af91474cd8f7-at-microscopy.com}
14, 52 -- Date: Sun, 8 May 2016 14:22:42 -0500
14, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
14, 52 -- Gecko/20100101 Thunderbird/45.0
14, 52 -- MIME-Version: 1.0
14, 52 -- In-Reply-To: {201605081644.u48GiGY8011428-at-ns.microscopy.com}
14, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 8, 2016 at 8:40:51 AM CDT
Subject: [Microscopy] viaWWW:Free Megaview II CCD System for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Hobie-at-technicalsalessolutions.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
Hobie-at-technicalsalessolutions.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: Hobie-at-technicalsalessolutions.com
Name: Hobie Richards

Organization: Technical Sales Solutions

Title-Subject: [Filtered] Free Megaview II CCD System for TEM

Message: TSS has one complete Megaview II CCD Side-mount Camera for Philips/FEI Series TEMs that
needs to find a home. Functional and complete side-mount CCD camera solution with computer and
cable set.
Pictures may be seen of the camera/flange here:

https://tss.exavault.com/share/view/bemn-fgdqg1k7

Will carefully prepare for shipment and with your shipping label and send to you anywhere in the world.


Login Host: 173.164.81.170
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
16, 52 -- From microscopy.listserver-at-gmail.com Sun May 8 08:40:45 2016
16, 52 -- Received: from mail-io0-f173.google.com (mail-io0-f173.google.com [209.85.223.173])
16, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u48Deioi019675
16, 52 -- for {microscopy-at-microscopy.com} ; Sun, 8 May 2016 08:40:44 -0500
16, 52 -- Received: by mail-io0-f173.google.com with SMTP id d62so151875128iof.2
16, 52 -- for {microscopy-at-microscopy.com} ; Sun, 08 May 2016 06:43:46 -0700 (PDT)
16, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 52 -- d=gmail.com; s=20120113;
16, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
16, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
16, 52 -- bh=WId7LMhEN1oRcQ5wPmuHe3Y1ON4L/WdSxalmaeGHSfg=;
16, 52 -- b=emjzKFR4g1CWSUoVbOuQY0wjVixWoOwB/lebCmWEVz5PhJt/YBPOjpoKRQh2DqQQX8
16, 52 -- 1gJs1CXrCkUgIcRUErVBrk+qmHBBVK7pV/t73gquKiUAAhJjhkeuLhDT28GMBfoyKf2k
16, 52 -- TnEPdUkraqRs25Dw9kw1zgjF36J1hdoYF+Et1vL+pWdNoZ8goIvy+AuCJKulG/wxheY5
16, 52 -- V7AgZxAYoTxQSi1Dp8ye7ZjjeHmG8PnCcHgy5RmvqAuTIhhpSdvu0I5dC40T1ZsqN9nq
16, 52 -- 2y5DHu9sfoIVU/VfNRum132lIoab463eMZ3IieyEkozrOEYEOobUeWQQMC9HtZw0i25G
16, 52 -- gSBQ==
16, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
16, 52 -- d=1e100.net; s=20130820;
16, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
16, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
16, 52 -- bh=WId7LMhEN1oRcQ5wPmuHe3Y1ON4L/WdSxalmaeGHSfg=;
16, 52 -- b=j1YAdu4lEe36LgTUuFRv87QSZtUfuVFp+iVNOuF60SIHCdLSvEDl2HlV/pUI9tZVes
16, 52 -- CaFiAc17FqqyDLWXaQUtD7IIK2ANUJDo55cNIjLPiT+okjeeMKHMxdPoxXd/VihFGrYh
16, 52 -- RORTpSEg9m4C+iJzVsXBv5FVNMJCyixCjqigAN03afpps+lj76R5SaZlsnty0TPG0J07
16, 52 -- /mlx/BAGiFwGkxE34uhEev/INTS/QiOco1/Stollxtnm923fRNnCQgknj/iDbu6Qqumr
16, 52 -- oxWC6ts7b5KBuDtiHZD7RgqZPxVvKiOPLLMuPzJS6NQqFKg/vwM0o2sHeS4vQqLn0Jns
16, 52 -- Wm+w==
16, 52 -- X-Gm-Message-State: AOPr4FWa242USsaGKsyyWOnyo/n9PMC5J1mMbwjevljA/iSimqypXmEB6u4i3K7e2h91hw==
16, 52 -- X-Received: by 10.107.180.198 with SMTP id d189mr32880921iof.187.1462715025840;
16, 52 -- Sun, 08 May 2016 06:43:45 -0700 (PDT)
16, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
16, 52 -- by smtp.googlemail.com with ESMTPSA id w24sm11391585ioi.32.2016.05.08.06.43.45
16, 52 -- for {microscopy-at-microscopy.com}
16, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
16, 52 -- Sun, 08 May 2016 06:43:45 -0700 (PDT)
16, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
16, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
16, 52 -- {microscopylistserver-noreply-at-microscopy.com}
16, 52 -- Subject: viaWWW:Free Megaview II CCD System for TEM
16, 52 -- References: {201605061659.u46GxxV8016452-at-ns.microscopy.com}
16, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
16, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
16, 52 -- X-Forwarded-Message-Id: {201605061659.u46GxxV8016452-at-ns.microscopy.com}
16, 52 -- Message-ID: {8fdaa9a5-4308-d37a-f941-9e8285b831dd-at-microscopy.com}
16, 52 -- Date: Sun, 8 May 2016 08:43:44 -0500
16, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
16, 52 -- Gecko/20100101 Thunderbird/45.0
16, 52 -- MIME-Version: 1.0
16, 52 -- In-Reply-To: {201605061659.u46GxxV8016452-at-ns.microscopy.com}
16, 52 -- Content-Type: text/plain; charset=UTF-8; format=flowed
16, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 6, 2016 at 9:23:11 AM CDT
Subject: [Microscopy] RE: viaWWW: justification of industrial user fee in a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Like you say, we take a look at local rates for similar services and price = higher so as to not undercut the local business.

If anyone gives me a hard time about rates I compare it to what they would = pay a professional photographer to take high school senior pictures or wedd= ing photos. EM Lab rates start to look entirely reasonable, especially give= n our "camera" is on the order of hundreds of thousands of dollars instead = of hundreds of dollars.

Kind regards,

William Stonewall Monroe
University of Alabama -at- Birmingham SEM Lab
http://www.uab.edu/scanningelectronmicroscopy


==============================Original Headers==============================
5, 29 -- From wsmonroe-at-uab.edu Fri May 6 09:23:11 2016
5, 29 -- Received: from UABEX7.ad.uab.edu (uabex7.ad.uab.edu [138.26.5.110])
5, 29 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u46ENA0c024567
5, 29 -- for {Microscopy-at-Microscopy.com} ; Fri, 6 May 2016 09:23:11 -0500
5, 29 -- Received: from UABEX2.ad.uab.edu (138.26.5.143) by UABEX7.ad.uab.edu
5, 29 -- (138.26.5.110) with Microsoft SMTP Server (TLS) id 15.0.1130.7; Fri, 6 May
5, 29 -- 2016 09:26:04 -0500
5, 29 -- Received: from UABEX2.ad.uab.edu ([138.26.5.143]) by UABEX2.ad.uab.edu
5, 29 -- ([138.26.5.143]) with mapi id 15.00.1130.005; Fri, 6 May 2016 09:26:05 -0500
5, 29 -- From: William Stonewall Monroe {wsmonroe-at-uab.edu}
5, 29 -- To: "shouliang.zhang-at-gmail.com" {shouliang.zhang-at-gmail.com} ,
5, 29 -- "'Microscopy-at-Microscopy.com'" {Microscopy-at-Microscopy.com}
5, 29 -- Subject: RE: [Microscopy] viaWWW: justification of industrial user fee in a
5, 29 -- university facility
5, 29 -- Thread-Topic: [Microscopy] viaWWW: justification of industrial user fee in a
5, 29 -- university facility
5, 29 -- Thread-Index: AdGnok2u3taNDirYRI6zNJwqJN1qdwAAMktg
5, 29 -- Date: Fri, 6 May 2016 14:26:05 +0000
5, 29 -- Message-ID: {1dcb3637dc584d7986e773c993c4bb07-at-UABEX2.ad.uab.edu}
5, 29 -- Accept-Language: en-US
5, 29 -- Content-Language: en-US
5, 29 -- X-MS-Has-Attach:
5, 29 -- X-MS-TNEF-Correlator:
5, 29 -- x-ms-exchange-transport-fromentityheader: Hosted
5, 29 -- x-originating-ip: [138.26.80.190]
5, 29 -- Content-Type: text/plain; charset="us-ascii"
5, 29 -- MIME-Version: 1.0
5, 29 -- Content-Transfer-Encoding: 8bit
5, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u46ENA0c024567
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 8:16:26 PM CDT
Subject: [Microscopy] viaWWW:justification of industrial user fee in a uiversity facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: shouliang.zhang-at-gmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
shouliang.zhang-at-gmail.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: shouliang.zhang-at-gmail.com Name: Shouliang Zhang

Title-Subject: [Filtered] justification of industrial user fee in a uiversity facility
Message: Dear listers,
How do your facilities, especially for the electron microscopes, justify the industrial user rate?
Do you consider the local analytical companies rate so that yours won't undercut theirs? I looked up
the SEM/TEM rate across the universties nationwide and found it varies a lot, ranging from less
$100/h to $300/h for industrial users. Thanks.

Login Host: 146.6.73.250
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
10, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 20:16:25 2016
10, 52 -- Received: from mail-qg0-f44.google.com (mail-qg0-f44.google.com [209.85.192.44])
10, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u461GPbK024302
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 20:16:25 -0500
10, 52 -- Received: by mail-qg0-f44.google.com with SMTP id f92so49409392qgf.0
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 18:19:18 -0700 (PDT)
10, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=gmail.com; s=20120113;
10, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
10, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=SDeVEu/IJUmDBKJGbWPt3Vw9+AGmU17Cu4hF+DptxCE=;
10, 52 -- b=fQ8BJ3dKsgXkISNj1F7oymt9I/vYRclrSDoCezWviCZ6tk/UDlURrZEGyvMtvqibX8
10, 52 -- QMAL+Hor5mIbbwwnYyNEGFWl35xXCUO6tFujxoZm2ixEUpDR1ekxnyBrVYK+tAJYKdWi
10, 52 -- oVsnu1yHtpXCoY7P8GWlV9wCDpm32h0zyAYMDti6vh3Cd8/InnxH+haX1ZiLZr0OPH9d
10, 52 -- Z70xUjUGk7TaXuQUWvXEUidTGIUkoZirinx0kbRZnhKozBQ7GxEgaKZatm68H+s6iXTt
10, 52 -- 2RrcoKOmvormf1RQ4Lza3wKH5APsH+eZ0XMgmQH9fL8AEckZkGlua2RDY6oxZy0pFSC2
10, 52 -- mrng==
10, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=1e100.net; s=20130820;
10, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
10, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=SDeVEu/IJUmDBKJGbWPt3Vw9+AGmU17Cu4hF+DptxCE=;
10, 52 -- b=BLJLq3IuTYAtaqeYF6A2mLk81ZByE6eFW4Qe3LjPqPu5VqWL53LHy2LCtuJwhQY1Ja
10, 52 -- VvC5WUdG4eysRt5VJSIW+EkoBvpD3lrtn9hd09TPdN1FshILQyAFsuoaDTWr0A+a/CNO
10, 52 -- 5FG0mGIjaliyBr/8cHhVBjOQJlSyEYlN8jAJCGvbSykdYawcy+Yrtn4LDuYofenkU6b9
10, 52 -- GiatPekKT9AwWkBAc57eennrQRkh5oJDwgyW+k9OLs6HQTkZ/jFFmh3Px7WHP6PhMwZe
10, 52 -- lOoR1N3FIXG8AXUBs0V0nOpEqxuERu+p3QU6XKggQZjziqTa4GHKpJ362C5CqjTc6/fd
10, 52 -- QE2g==
10, 52 -- X-Gm-Message-State: AOPr4FUnms36E2y6Op368tFk6PCdzGcWmv7QzVQlHlvu5dwyafpGmlUn5tb+3V+M5xb+zQ==
10, 52 -- X-Received: by 10.140.22.203 with SMTP id 69mr2840140qgn.62.1462497557768;
10, 52 -- Thu, 05 May 2016 18:19:17 -0700 (PDT)
10, 52 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1])
10, 52 -- by smtp.googlemail.com with ESMTPSA id c33sm4694733qga.39.2016.05.05.18.19.16
10, 52 -- for {microscopy-at-microscopy.com}
10, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
10, 52 -- Thu, 05 May 2016 18:19:17 -0700 (PDT)
10, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
10, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
10, 52 -- {microscopylistserver-noreply-at-microscopy.com}
10, 52 -- Subject: viaWWW:justification of industrial user fee in a uiversity facility
10, 52 -- References: {201605052145.u45Ljrio019548-at-ns.microscopy.com}
10, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
10, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
10, 52 -- X-Forwarded-Message-Id: {201605052145.u45Ljrio019548-at-ns.microscopy.com}
10, 52 -- Message-ID: {4bb26c2f-1c69-d2f5-6069-af4d0aefb8dd-at-microscopy.com}
10, 52 -- Date: Thu, 5 May 2016 20:19:16 -0500
10, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
10, 52 -- Gecko/20100101 Thunderbird/45.0
10, 52 -- MIME-Version: 1.0
10, 52 -- In-Reply-To: {201605052145.u45Ljrio019548-at-ns.microscopy.com}
10, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 1:47:38 PM CDT
Subject: [Microscopy] Electrical feedthroughs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

If you are not fussy or fancy just drive some sharpened 1/8" diameter
stainless steel rods through a clean rubber stopper. Will work OK
through the HV range (10^-6 Torr) , but probably not into the UHV range.

==============================Original Headers==============================
1, 42 -- From bigelow-at-umich.edu Thu May 5 13:47:37 2016
1, 42 -- Received: from mail-wm0-f46.google.com (mail-wm0-f46.google.com [74.125.82.46])
1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45Ilbls023188
1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 13:47:37 -0500
1, 42 -- Received: by mail-wm0-f46.google.com with SMTP id a17so42508274wme.0
1, 42 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 11:50:28 -0700 (PDT)
1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 42 -- d=umich.edu; s=google;
1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject
1, 42 -- :content-transfer-encoding;
1, 42 -- bh=3bbbWfX1sDpnKVTCO89Hjj6HEN6J/n3Vy9oX4WnYMco=;
1, 42 -- b=PN2s3z03LUl1lZCMHVkAk6dMHP+JXoAvKck86xJqHqeaeCYek/hpDUFtu4flG8dyoh
1, 42 -- BwRgQkEtLZPAF9TTyNwVxbjI15hWNxMuE8/YnFQ7McADcY34/OsFLzYcLT3StJTlUE6U
1, 42 -- V6k4NJoCHQ1wxN87xkNFHw9FHJcyI3Lju0TJc=
1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 42 -- d=1e100.net; s=20130820;
1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to
1, 42 -- :subject:content-transfer-encoding;
1, 42 -- bh=3bbbWfX1sDpnKVTCO89Hjj6HEN6J/n3Vy9oX4WnYMco=;
1, 42 -- b=JqeRpKe/fqzx8vZr+8gHGkt49U/a/Eo/oFeKCujXMVUUI+A97c5wTFBGt8/1ib4Bkt
1, 42 -- wlIYewSTHCTzivwrzGhSbwcqO2P52qREVVx1rqEzE4ytHh3UKE/RKQqoFa1HlOqo/ofJ
1, 42 -- zLllgfb3z38RieqYFUPVTInWguY9gjusF2NIxNxP6ADgkJ3Frr4fKSCNiOWxjdznnRgM
1, 42 -- x41jLxNcbfQFq0F6TBQg4KTS6d96Czotn7Lu9Llw6VwWXqHIF4b+JCYPrSGteiMtUCKx
1, 42 -- jbFCBjICyucgqPeAgm9sbTCIBJ69Q7t8WXBQJE75A97toiqercgV4qlr5Lqqlz1idhyb
1, 42 -- pB4w==
1, 42 -- X-Gm-Message-State: AOPr4FUd9PlZ9IePDj8akeu3j5TZchle2s1JpKnDShGEsCOLRjQ503pse6ojUlPCeoI6I0JL
1, 42 -- X-Received: by 10.194.139.104 with SMTP id qx8mr15321433wjb.14.1462474228130;
1, 42 -- Thu, 05 May 2016 11:50:28 -0700 (PDT)
1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c])
1, 42 -- by smtp.gmail.com with ESMTPSA id i194sm4492750wmf.6.2016.05.05.11.50.27
1, 42 -- for {microscopy-at-microscopy.com}
1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128);
1, 42 -- Thu, 05 May 2016 11:50:27 -0700 (PDT)
1, 42 -- Message-ID: {572B95F1.1060904-at-umich.edu}
1, 42 -- Date: Thu, 05 May 2016 14:50:25 -0400
1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
1, 42 -- MIME-Version: 1.0
1, 42 -- To: microscopy-at-microscopy.com
1, 42 -- Subject: [Microscopy]Electrical feedthroughs
1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
1, 42 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 12:42:03 PM CDT
Subject: [Microscopy] Re: viaWWW:electrical pass through for FEI ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My friend recently made a feedthrough using stiff piano wire, a rubber
stopper from home depot, and super glue (I think, or maybe it was
torr-seal type epoxy). He used a DB9 port to mark the pin locations,
then drilled pilot holes (which I don't think were fully drilled
through the rubber) and I believe then he pounded the wire through the
remainder of the rubber. This stopper was jammed into a tapered hole
in a blank/unused blocking plate that came with his machine. It holds
working pressure as good as before the feedthrough was installed. If
you want more info, I can probably pass you his direct email to ask
for more details.

On Thu, May 5, 2016 at 5:39 AM, {microscopy.listserver-at-gmail.com} wrote:



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

X-from: wambrose-at-unc.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
wambrose-at-unc.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: wambrose-at-unc.edu Name: Wallace Ambrose

Organization: CHANL UNC

Title-Subject: [Filtered] electrical pass through for FEI ESEM
Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and
Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what
connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports.
thanks
Wallace
Login Host: 152.19.204.249
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
10, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 06:40:33 2016
10, 52 -- Received: from mail-ig0-f178.google.com (mail-ig0-f178.google.com [209.85.213.178])
10, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45BeWDh005754
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 06:40:32 -0500
10, 52 -- Received: by mail-ig0-f178.google.com with SMTP id u5so2215544igk.1
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 04:43:23 -0700 (PDT)
10, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=gmail.com; s=20120113;
10, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
10, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=aY+PLLOam++TMR89ihZ2NO/vIflOTtWlJ+LXxXUwuZ4=;
10, 52 -- b=Gwhll4fUYiOKsJMxJ3YQEtBfkKEMCXQz7ORUbmDPjUATun2PfhlUfuuFlZTgM2Fp1z
10, 52 -- bqJLR/kwqqAmHeqwpFUaikRgAbiIgrO/bg0YCLLh5G/wrSSx2b+/dawrq37OIzAgLMZ/
10, 52 -- Zgm9wO5TrjMtJLZDG/KDq887RkMdB5IJUQidrj9zxmYAnJHsudzlujiIl9Yt7Gy6QMOE
10, 52 -- G26WFkpEXjjNG60KQStlUcmh7nhV6VEhmxXgkcS2l45+l9xJrAYYKOHVSJ1nXo91KRfK
10, 52 -- cR6jYMjOZUmC6ceuEp1Wxu6MwU4q6f5xBMR50b3NLTF+6OR5fzIJJjELQbwaoRJtzgUE
10, 52 -- jwUg==
10, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=1e100.net; s=20130820;
10, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
10, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=aY+PLLOam++TMR89ihZ2NO/vIflOTtWlJ+LXxXUwuZ4=;
10, 52 -- b=Bk9LSPd2V1vx34qMw40vmJyIHPZbEwmdRqCk4Io4Rwy8F0uUjnJPuFbZeQtrMNqeOm
10, 52 -- zfHt3/+1X+6wHCGOhlpQF+nQxO5R5Fyoo26XX5eS0NamNdJ5UeE748FLzNgP5F84nbsx
10, 52 -- d0KpJH1DQNDl7gfVZn44uaDKmUAlVKkdnWogTdXOv8Cw0xNytPSMx9Pn4bRqNGwLLoEH
10, 52 -- 6O3JRVy7OLpA1kXjt5ByEzkc35ktX65pkvwVV0IxS5peidvjzHWum4kvdAElMzp0iZDp
10, 52 -- hiw8a1CP4b5InPvu6Kk0I93ONFydGEM2nrE/IMhsnc2H4hnbHCXt3zr8L+L01J5dUgrK
10, 52 -- hJug==
10, 52 -- X-Gm-Message-State: AOPr4FXbVSEuMrKSdVWIgQfvZM8zNP3dOra+923XBCxUagNKG5fiFhCdrhZQv5ZI13MWEA==
10, 52 -- X-Received: by 10.50.101.131 with SMTP id fg3mr2893477igb.75.1462448602985;
10, 52 -- Thu, 05 May 2016 04:43:22 -0700 (PDT)
10, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
10, 52 -- by smtp.googlemail.com with ESMTPSA id uy5sm1322886igc.4.2016.05.05.04.43.22
10, 52 -- for {microscopy-at-microscopy.com}
10, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
10, 52 -- Thu, 05 May 2016 04:43:22 -0700 (PDT)
10, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
10, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
10, 52 -- {microscopylistserver-noreply-at-microscopy.com}
10, 52 -- Subject: viaWWW:electrical pass through for FEI ESEM
10, 52 -- References: {201605021440.u42Eec1w000375-at-ns.microscopy.com}
10, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
10, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
10, 52 -- X-Forwarded-Message-Id: {201605021440.u42Eec1w000375-at-ns.microscopy.com}
10, 52 -- Message-ID: {f00a74fe-3cbb-75cd-2434-02f76d8300f8-at-microscopy.com}
10, 52 -- Date: Thu, 5 May 2016 06:43:21 -0500
10, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
10, 52 -- Gecko/20100101 Thunderbird/45.0
10, 52 -- MIME-Version: 1.0
10, 52 -- In-Reply-To: {201605021440.u42Eec1w000375-at-ns.microscopy.com}
10, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 6:43:39 AM CDT
Subject: [Microscopy] viaWWW:Cryomicrotomy using glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: duraine-at-bcm.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
duraine-at-bcm.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: duraine-at-bcm.edu Name: Lita Duraine

Organization: Howard Hughes Medical Institute

Title-Subject: [Microscopy] RE: Cryomicrotomy using glass knives

Message: Hi Ondrej,

At Delta microscopy school some of us used 3M Silver Polyester Tape, Nonconductive, 850 from Ted
Pella to make boats for glass knives. I think the tape is rated for -150 degrees C. Ted Pella also
carries other type tapes that may be used for making boats with lower temp ranges. You might check.

Regards,

Lita Duraine
Login Host: 128.249.1.198
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 06:43:38 2016
14, 52 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171])
14, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45Bhcia008196
14, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 06:43:38 -0500
14, 52 -- Received: by mail-ig0-f171.google.com with SMTP id bi2so12754710igb.0
14, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 04:46:28 -0700 (PDT)
14, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=gmail.com; s=20120113;
14, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=1dNmQcgz5gONL6M/FsmkLFYHsv1MtMORs8AzO1299Tc=;
14, 52 -- b=gFFb90Yjf9HIJkBxyzhvmALIjDXd/R/TbMFPgZQZWKvsPHAIb/tjQ37P7jsXn/yxpU
14, 52 -- PTic+GzP/KehC1fInaql0TbfyuL4JbK2IAl6DA2F38tKWOq23cO6XALipHkw/qGkRGev
14, 52 -- XuTxQe6S5u826cAWlCLQkuegRZQ+fYMl9FikIRFML9W3UnBtwQx2VLs1kI3k/Oh27T2O
14, 52 -- enjp1j6kHwk3mR4vtRqpooY9HSB7fs7P8ODNjT0J9zFzrkz4xTNhOq3Qw5Jcj9f+HVSg
14, 52 -- MBEmy0yXY/TNrtkQo6wYA3Eb87hEkNjB7hRwP9V3ZBzzCSCBy64eEBfyRL94Hev2Q9U/
14, 52 -- gmxg==
14, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 52 -- d=1e100.net; s=20130820;
14, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
14, 52 -- bh=1dNmQcgz5gONL6M/FsmkLFYHsv1MtMORs8AzO1299Tc=;
14, 52 -- b=WLGycEr0rGWOqaXeqkh97vTvHGv8n0NDR/DzE4hjvvWNP2M3dLlxFZZqZzCJrpx55F
14, 52 -- lTS0T7ZVk+kUNG1RoOI6EPsCCxoaYZ8ilVMU2aFoxCZWXywHipbmJVOZCvjr44Xaw8vr
14, 52 -- 5P1GkeZr+VDMFVBLjnQgO/+VjYxDPgan1Nvetty7ly70YItL2cC6UBekN3a8KSxDPEF/
14, 52 -- zicxZs4wDCB8Fn4nauJDXUiaX6hCnRLFqhDOEtVqdnFqG3hkp3NJMCOikv26yfA/FOw5
14, 52 -- M4BRI7DHQPyLXKONhOYJx1XUTJ2yFJjv2F/vHz8Mv3dDg/6Ab+sXjsFiNZm+mrXJN3Mj
14, 52 -- 2rnQ==
14, 52 -- X-Gm-Message-State: AOPr4FVYjlrRKIjOLSe6x7Vh1Vk/XT9u4+OYafcg8sen5B7AHn7xOBDrN2xQjaPOpBYl2Q==
14, 52 -- X-Received: by 10.50.3.73 with SMTP id a9mr3051880iga.22.1462448788693;
14, 52 -- Thu, 05 May 2016 04:46:28 -0700 (PDT)
14, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 52 -- by smtp.googlemail.com with ESMTPSA id j84sm3809349ioo.5.2016.05.05.04.46.28
14, 52 -- for {microscopy-at-microscopy.com}
14, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 52 -- Thu, 05 May 2016 04:46:28 -0700 (PDT)
14, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 52 -- {microscopylistserver-noreply-at-microscopy.com}
14, 52 -- Subject: viaWWW:Cryomicrotomy using glass knives
14, 52 -- References: {201605042214.u44MEvbt005268-at-ns.microscopy.com}
14, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 52 -- X-Forwarded-Message-Id: {201605042214.u44MEvbt005268-at-ns.microscopy.com}
14, 52 -- Message-ID: {00693df4-ec8a-7765-f992-2e72b53e9c51-at-microscopy.com}
14, 52 -- Date: Thu, 5 May 2016 06:46:27 -0500
14, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
14, 52 -- Gecko/20100101 Thunderbird/45.0
14, 52 -- MIME-Version: 1.0
14, 52 -- In-Reply-To: {201605042214.u44MEvbt005268-at-ns.microscopy.com}
14, 52 -- Content-Type: text/plain; charset=UTF-8; format=flowed
14, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 6:42:44 AM CDT
Subject: [Microscopy] viaWWW:Importing .txt files into Digital Micrograph for EELS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: peter.eschbach-at-oregonstate.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
peter.eschbach-at-oregonstate.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: peter.eschbach-at-oregonstate.edu Name: Peter Eschbach

Organization: Oregon State University

Title-Subject: [Filtered] Importing .txt files into Digital Micrograph for EELS

Message: Hi:

We do not have a digiscan box to control our Titan from Digital Micrograph (DM). So, for now we are
collecting simultaneous EELS and EDX linescans in FEI's TIA software. I can do rudimentary analysis
in TIA on all the EELS data but would like to import several EELS spectra from my linescan into DM.
I am able to save the TIA acquired EELS data into .txt files with two nice columns, Energy and
Intensity. But, does anybody know of a script to convert the .txt files into DM3? We looked on
David Mitchell's site, but nothing jumped out.
Thanks,
Pete Eschbach
Oregon State University EM Facility
541 737 5645

Login Host: 128.193.93.151
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
13, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 06:42:43 2016
13, 52 -- Received: from mail-io0-f180.google.com (mail-io0-f180.google.com [209.85.223.180])
13, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45BghqV007087
13, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 06:42:43 -0500
13, 52 -- Received: by mail-io0-f180.google.com with SMTP id 190so81847124iow.1
13, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 04:45:33 -0700 (PDT)
13, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 52 -- d=gmail.com; s=20120113;
13, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
13, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
13, 52 -- bh=gUVNTLyEArKVogxo/rmc16RNU2jfoqP91pxGms+pk9Y=;
13, 52 -- b=avMP7aWxC/ODNYcnsXUhoWjKoCcIgY6tEv48BojvjhFbaqok7pD5J7jIfHj15vG5KO
13, 52 -- uf8q420wekVOtWkan4UyUL5G7CLiCnMu4zrC2qN4l76ne1vXNiyOYvk1Y/524iD4H/1m
13, 52 -- xvYzgHN4Odk+B6DhPysxYkhPQxkpkg9LeEwyrsSpaLVu67lnvI68UCislaPYpy9xD5hX
13, 52 -- wWpo1Fy+zvv5whC98+h/Zd/nXxPIfD6dfIK2bph93tI8AMVgRu4HZ14SO/aj8S0bYZgQ
13, 52 -- Pxek9Lk5FqudrEgVQT2cSMOB72uN38XqZY//MGoBwDPH9rqwziOspY0fWo8ezkJtTmYq
13, 52 -- rQkQ==
13, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 52 -- d=1e100.net; s=20130820;
13, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
13, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
13, 52 -- bh=gUVNTLyEArKVogxo/rmc16RNU2jfoqP91pxGms+pk9Y=;
13, 52 -- b=SHlu872BJyQv2qhX+J1/o246odujEA6m8xL/pFleMuLE7asUFDbbhucYFBAU11xf/z
13, 52 -- GCUTzLpSD6/iji1jIBo7u8DBCETWDfTK2P9W5hH6bNu7FhB1yvMLhRQUxYsuFDSv3cQ1
13, 52 -- hGgv8+AYhADs67W5tklkfAEkyeoyrw1H2pCK9n0rBRrju7wIY/Ljk1QywAvFtPtBrT8g
13, 52 -- bA12+MI+qLSOlVUTWwhxaK1KIjagT6roLpyxMOYSR5+lEouIqjWzsrdKldoYsLaS/G/p
13, 52 -- DyDi//uBerfFOnBvss8HranAMdZpXeDoX9+wAI7QF0gLbYKr0AYj8sZVknvh79kFfkcr
13, 52 -- CPTA==
13, 52 -- X-Gm-Message-State: AOPr4FWRR0IopBFt1ZM00HcNPeZDVEMDQYpqpnG9EoMGSyUt1C6QqHe969r7eIa4m55tlg==
13, 52 -- X-Received: by 10.107.37.16 with SMTP id l16mr15458219iol.138.1462448733362;
13, 52 -- Thu, 05 May 2016 04:45:33 -0700 (PDT)
13, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
13, 52 -- by smtp.googlemail.com with ESMTPSA id yb2sm1325559igc.9.2016.05.05.04.45.32
13, 52 -- for {microscopy-at-microscopy.com}
13, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 52 -- Thu, 05 May 2016 04:45:33 -0700 (PDT)
13, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
13, 52 -- {microscopylistserver-noreply-at-microscopy.com}
13, 52 -- Subject: viaWWW:Importing .txt files into Digital Micrograph for EELS
13, 52 -- References: {201605042124.u44LOUb9004526-at-ns.microscopy.com}
13, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
13, 52 -- X-Forwarded-Message-Id: {201605042124.u44LOUb9004526-at-ns.microscopy.com}
13, 52 -- Message-ID: {27cdd6f3-4ec6-72b2-1113-0a454ede8385-at-microscopy.com}
13, 52 -- Date: Thu, 5 May 2016 06:45:32 -0500
13, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
13, 52 -- Gecko/20100101 Thunderbird/45.0
13, 52 -- MIME-Version: 1.0
13, 52 -- In-Reply-To: {201605042124.u44LOUb9004526-at-ns.microscopy.com}
13, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 5, 2016 at 6:40:33 AM CDT
Subject: [Microscopy] viaWWW:electrical pass through for FEI ESEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: chrisbrantner-at-email.gwu.edu

X-from: wambrose-at-unc.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying please copy both
wambrose-at-unc.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: wambrose-at-unc.edu Name: Wallace Ambrose

Organization: CHANL UNC

Title-Subject: [Filtered] electrical pass through for FEI ESEM
Message: Hello, I would like to do voltage contrast and active EBIC on our FEI Quanta ESEM and
Helios FIB. I am interested in knowing what folks have done to make up pass through plates (and what
connectors (BNC, DB9 or DB25) would be the most useful for this for the 4 or 2 3/8 inch ports.
thanks
Wallace
Login Host: 152.19.204.249
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
10, 52 -- From microscopy.listserver-at-gmail.com Thu May 5 06:40:33 2016
10, 52 -- Received: from mail-ig0-f178.google.com (mail-ig0-f178.google.com [209.85.213.178])
10, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u45BeWDh005754
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 5 May 2016 06:40:32 -0500
10, 52 -- Received: by mail-ig0-f178.google.com with SMTP id u5so2215544igk.1
10, 52 -- for {microscopy-at-microscopy.com} ; Thu, 05 May 2016 04:43:23 -0700 (PDT)
10, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=gmail.com; s=20120113;
10, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
10, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=aY+PLLOam++TMR89ihZ2NO/vIflOTtWlJ+LXxXUwuZ4=;
10, 52 -- b=Gwhll4fUYiOKsJMxJ3YQEtBfkKEMCXQz7ORUbmDPjUATun2PfhlUfuuFlZTgM2Fp1z
10, 52 -- bqJLR/kwqqAmHeqwpFUaikRgAbiIgrO/bg0YCLLh5G/wrSSx2b+/dawrq37OIzAgLMZ/
10, 52 -- Zgm9wO5TrjMtJLZDG/KDq887RkMdB5IJUQidrj9zxmYAnJHsudzlujiIl9Yt7Gy6QMOE
10, 52 -- G26WFkpEXjjNG60KQStlUcmh7nhV6VEhmxXgkcS2l45+l9xJrAYYKOHVSJ1nXo91KRfK
10, 52 -- cR6jYMjOZUmC6ceuEp1Wxu6MwU4q6f5xBMR50b3NLTF+6OR5fzIJJjELQbwaoRJtzgUE
10, 52 -- jwUg==
10, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 52 -- d=1e100.net; s=20130820;
10, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
10, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
10, 52 -- bh=aY+PLLOam++TMR89ihZ2NO/vIflOTtWlJ+LXxXUwuZ4=;
10, 52 -- b=Bk9LSPd2V1vx34qMw40vmJyIHPZbEwmdRqCk4Io4Rwy8F0uUjnJPuFbZeQtrMNqeOm
10, 52 -- zfHt3/+1X+6wHCGOhlpQF+nQxO5R5Fyoo26XX5eS0NamNdJ5UeE748FLzNgP5F84nbsx
10, 52 -- d0KpJH1DQNDl7gfVZn44uaDKmUAlVKkdnWogTdXOv8Cw0xNytPSMx9Pn4bRqNGwLLoEH
10, 52 -- 6O3JRVy7OLpA1kXjt5ByEzkc35ktX65pkvwVV0IxS5peidvjzHWum4kvdAElMzp0iZDp
10, 52 -- hiw8a1CP4b5InPvu6Kk0I93ONFydGEM2nrE/IMhsnc2H4hnbHCXt3zr8L+L01J5dUgrK
10, 52 -- hJug==
10, 52 -- X-Gm-Message-State: AOPr4FXbVSEuMrKSdVWIgQfvZM8zNP3dOra+923XBCxUagNKG5fiFhCdrhZQv5ZI13MWEA==
10, 52 -- X-Received: by 10.50.101.131 with SMTP id fg3mr2893477igb.75.1462448602985;
10, 52 -- Thu, 05 May 2016 04:43:22 -0700 (PDT)
10, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
10, 52 -- by smtp.googlemail.com with ESMTPSA id uy5sm1322886igc.4.2016.05.05.04.43.22
10, 52 -- for {microscopy-at-microscopy.com}
10, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
10, 52 -- Thu, 05 May 2016 04:43:22 -0700 (PDT)
10, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
10, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
10, 52 -- {microscopylistserver-noreply-at-microscopy.com}
10, 52 -- Subject: viaWWW:electrical pass through for FEI ESEM
10, 52 -- References: {201605021440.u42Eec1w000375-at-ns.microscopy.com}
10, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
10, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
10, 52 -- X-Forwarded-Message-Id: {201605021440.u42Eec1w000375-at-ns.microscopy.com}
10, 52 -- Message-ID: {f00a74fe-3cbb-75cd-2434-02f76d8300f8-at-microscopy.com}
10, 52 -- Date: Thu, 5 May 2016 06:43:21 -0500
10, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:45.0)
10, 52 -- Gecko/20100101 Thunderbird/45.0
10, 52 -- MIME-Version: 1.0
10, 52 -- In-Reply-To: {201605021440.u42Eec1w000375-at-ns.microscopy.com}
10, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
10, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 7:24:33 PM CDT
Subject: [Microscopy] Pump speed for wafer holder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Don:
A pumping speed of 1 cfm is a pretty high rate of flow (28 liters/min)
and typical of values for small rotary vane pumps. For this type of pump
the speed is usually rated as the volume of gas moved through the pump
when both the inlet and outlet ports are at atmospheric presure. I
wonder if you would really need a pumping speed of 1 cfm for a device
that merely holds a wafer in place. The question is, what is the actual
volume that needs to be evacuated, and how much of a hurry are you in to
get the wafer held firmly in place. If you just need to evacuate a tube
of modest diameter and modest length leading to a small chamber under
the wafer you certainly don't need a speed of 1 cfm to do the job, even
if the system leaks pretty badly.

Wil

==============================Original Headers==============================
2, 42 -- From bigelow-at-umich.edu Wed May 4 19:24:32 2016
2, 42 -- Received: from mail-wm0-f44.google.com (mail-wm0-f44.google.com [74.125.82.44])
2, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u450OVBO002754
2, 42 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 19:24:32 -0500
2, 42 -- Received: by mail-wm0-f44.google.com with SMTP id a17so2635129wme.0
2, 42 -- for {microscopy-at-microscopy.com} ; Wed, 04 May 2016 17:27:20 -0700 (PDT)
2, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
2, 42 -- d=umich.edu; s=google;
2, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject
2, 42 -- :content-transfer-encoding;
2, 42 -- bh=hq58s9xkmj+ZbM9fQ2wwIYVZX1qv8u2pIM2DIldDGuc=;
2, 42 -- b=IcuIgLDQ5CQ/kyLNfMDtVoNXfzUT37OybdkPqO2V5iYhAYD7FYCiYV7v68vKxClQ9H
2, 42 -- OQ3G61zMM9K6Z+IwnIDM0/5rHL0vM9Yp9Nd8n8Ht5VWfAN99YrsgMKeZ4eLHpBk5OBHr
2, 42 -- V2KYYK9NiN9VK6AeIhTbKYk/WxED89i2fHy8I=
2, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
2, 42 -- d=1e100.net; s=20130820;
2, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to
2, 42 -- :subject:content-transfer-encoding;
2, 42 -- bh=hq58s9xkmj+ZbM9fQ2wwIYVZX1qv8u2pIM2DIldDGuc=;
2, 42 -- b=NKBQMOmf+dccqKPbu/tmIUz3H/ZLU+qKGpmzDtVD43h9LQfCiHKC2a1NzN0z2zU2T9
2, 42 -- LejstTbCX51qWliRLGYVsXyyKRIVvfmQrHwEOn97pDfzd67e+iEZJS9o8j3j2wiQkHh/
2, 42 -- +Lw4WSeQAb3XrkZzvngaGjABibh9LBUbMT92dXzw1IwC5Qog3hK7A/sEPyAUGuwgu3g5
2, 42 -- U3L2Uj7MlUuB+/pFB1TEUDCbh5Ka3QW7qYSwLpseMdUmQ2nTysDjQW6VYMPH283V81IZ
2, 42 -- /knMFuE6SqYNyuFTS9AcV3Ej2tEhcQoIz3Ja1cLjGHQcs6zzV8SjJh5fkt85iWzYtgtD
2, 42 -- b3Ng==
2, 42 -- X-Gm-Message-State: AOPr4FU8hFs4U2inG+8+tUz9dN4gs81y+zjx7L9vV6Jdxkl82deva3Eo3VjgTH5dm+69d1KA
2, 42 -- X-Received: by 10.194.187.236 with SMTP id fv12mr11238503wjc.96.1462408039588;
2, 42 -- Wed, 04 May 2016 17:27:19 -0700 (PDT)
2, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c])
2, 42 -- by smtp.gmail.com with ESMTPSA id on2sm6872092wjc.32.2016.05.04.17.27.18
2, 42 -- for {microscopy-at-microscopy.com}
2, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128);
2, 42 -- Wed, 04 May 2016 17:27:19 -0700 (PDT)
2, 42 -- Message-ID: {572A9365.600-at-umich.edu}
2, 42 -- Date: Wed, 04 May 2016 20:27:17 -0400
2, 42 -- From: Wil Bigelow {bigelow-at-umich.edu}
2, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
2, 42 -- MIME-Version: 1.0
2, 42 -- To: microscopy-at-microscopy.com
2, 42 -- Subject: [Microscopy]Pump speed for wafer holder
2, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
2, 42 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 3:13:57 PM CDT
Subject: [Microscopy] Re: how are vacuum pumps rated?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Fred,
Thanks very much for this explanation.
Don

----- Original Message -----
From: Frederick Schamber
To: Don Chernoff at ASM
Sent: Wednesday, May 04, 2016 5:31 PM
Subject: [a] Re: how are vacuum pumps rated?


Three things: (1) Vacuum pump ratings (pumping speed) always apply to the
inlet (evacuated).

(2) If they say cfm (or lps) it is 'pumping speed' and that is independent
of either internal or external pressure. Think of having a 'demon'
collecting one cubic foot of gas in a (previously evacuated) canister while
inside the chamber. The demon then seals the canister and removes it from
the chamber. To repeat the cycle he shrinks the canister to zero volume (
thereby expelling the collected gas) and then goes back into the chamber
where he expands it to 1 cubic foot again. If he does this once a minute,
that's a 1 cfm pumping speed. It's easiest to see that's what a piston pump
does but it's the same principle for rotary vane (and can be generalized for
a turbo). If you think of the 'demon' model it's clear that the volume per
second is a function only of the canister size and repetition rate, not the
pressure (either internal nor external. That's an idealization for an
actual pump of course due to things like leakage, turbulent flow, and lots
of other messy things. But in principle, volume pumping speed is constant.

(3) Mass flow rate is density times pumping speed and would be something
like grams per second. Since the density of the gas decreases, the mass
flow rate also decreases with improving vacuum. A pump's 'throughout' is
defined as inlet pressure x pumping speed and is proportional to mass flow
rate.

Actually, mass flow rate (and throughout) can be defined and is invariant
anywhere in the system, so long as volume and density are defined at the
same place. So you can use the constancy of throughput to calculate the
volume of gas exhausted for a given pumping speed and inlet pressure.

Any help?

Fred

Sent from my iPhone

On May 4, 2016, at 4:14 PM, Don Chernoff at ASM {donc-at-asmicro.com}
wrote:

Dear Fred,
Thanks for your reply. I you're pointing in the right direction. My
question is a practical one: if someone says "you need a flow rate of 1
cfm"
does that mean 1 cfm measured at P = 1 atmosphere?
Don
----- Original Message ----- From: Frederick Schamber
To: donc-at-asmicro.com
Sent: Wednesday, May 04, 2016 3:13 PM

Dear Fred,
Thanks for your reply. I you're pointing in the right direction. My
question is a practical one: if someone says "you need a flow rate of 1 cfm"
does that mean 1 cfm measured at P = 1 atmosphere?
Don
----- Original Message -----
From: Frederick Schamber
To: donc-at-asmicro.com
Sent: Wednesday, May 04, 2016 3:13 PM
Subject: [a] Re: [Microscopy] how are vacuum pumps rated?


Don,


Maybe I misunderstand your question, but I was long puzzled by how
mechanical vacuum pumps are rated. Finally I realized that the mechanism of
a vacuum pump will capture and remove a constant volume of gas regardless of
internal pressure. That is, regardless of that internal pressure, the
volume swept out remains the same. Clearly, that means that the volume of
gas exhausted decreases proportionately as the internal pressure declines.
This is why we use 'mass flow' when we want to quantify the mass of material
pumped.


So if you are looking at volume of gas removed from the chamber the
pumping speed is (essentially) constant. But if you are looking at volume
or mass of gas exhausted, the pumping speed varies proportionately to
internal pressure.


Does this help?


Fred Schamber (retired, but still interested)


On Wed, May 4, 2016 at 2:27 PM, {donc-at-asmicro.com} wrote:




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of
America
To Subscribe/Unsubscribe --
http://www.microscopy.com/MicroscopyListserver
On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
----------------------------------------------------------------------------

Dear All,
I want to thank the many people who responded to my initial post about
low
vacuum pump.

In reviewing vacuum pump specifications, I note that the flow rate chart
shows a high rate at atmospheric pressure with a rapid decrease in rate
as
the input pressure decreases. When this type of pump is specified, is
there
general agreement that the flow rate is specified for free air (at 1
atmosphere pressure)?
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM
probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================
----- Original Message -----
From: Don Chernoff at ASM
To: Microscopy List
Sent: Sunday, May 01, 2016 7:01 PM
Subject: low vacuum pump


I'm working with a wafer inspection system that uses vacuum to clamp
the
wafer. The manufacturer's specifications call for a modest vacuum:

low vacuum: 150 torr (-24" Hg)

flow rate: { 1 cfm (28 l/m)

Actual flow will be intermittent.



What type of pump would be suitable for this application?

I welcome suggestions for specific models and suggestions for how to
configure the pump.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM
probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


==============================Original
Headers==============================
13, 36 -- From donc-at-asmicro.com Wed May 4 12:57:52 2016
13, 36 -- Received: from resqmta-ch2-04v.sys.comcast.net
(resqmta-ch2-04v.sys.comcast.net [69.252.207.36])
13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with
ESMTP id u44HvqS3018310
13, 36 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016
12:57:52 -0500
13, 36 -- Received: from resomta-ch2-12v.sys.comcast.net
([69.252.207.108])
13, 36 -- by comcast with SMTP
13, 36 -- id y13Ra9nYenIc7y16GaqLzA; Wed, 04 May 2016 18:00:40
+0000
13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
d=comcast.net;
13, 36 -- s=q20140121; t=1462384840;
13, 36 -- bh=rLQcAZXBgVIgN7HzeuJJmjrOEuhcJgIo+KezbapneEQ=;
13, 36 --
h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version:
13, 36 -- Content-Type;
13, 36 --
b=EZxq9X3LngRIebRch8mPpB/+EHMSoIQTXQYgInpRqhMn6qoLloFkmxd+bfdLucEdY
13, 36 --
Q7OYd0p+Efi7CAdY9qmvsZG6GF3L3i/uGbRiA/ghjAApdavI27SVkjWek+9vYeMvUr
13, 36 --
I+CaDvaqxFz13X2g9+CK/+7OD25/UYhL8KDzjBoiiuCebz63GcZcK/bOjYXAMBIWD9
13, 36 --
vSou15Qy2M+YK67r0XaIpHTRpStMOolGJm+zBKlPHSp7glHk2ICdHEqKGekaUyCt64
13, 36 --
SxuldlG0YoMaYbNC9HTvYfFWv7Cd1ETZpsr9evQjdkKLAoxusa69b1sDhj7JDScml8
13, 36 -- 8NN+YcII9FAMA==
13, 36 -- Received: from asm20 ([68.58.83.137])
13, 36 -- by resomta-ch2-12v.sys.comcast.net with comcast
13, 36 -- id qJ0f1s00S2xmMPG01J0gSU; Wed, 04 May 2016 18:00:40
+0000
13, 36 -- Message-ID: {15E61CF10A444A239D5D085E7185F339-at-asm20}
13, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
13, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com}
13, 36 -- Subject: how are vacuum pumps rated?
13, 36 -- Date: Wed, 4 May 2016 14:00:41 -0400
13, 36 -- MIME-Version: 1.0
13, 36 -- Content-Type: text/plain;
13, 36 -- format=flowed;
13, 36 -- charset="iso-8859-1";
13, 36 -- reply-type=original
13, 36 -- Content-Transfer-Encoding: 7bit
13, 36 -- X-Priority: 3
13, 36 -- X-MSMail-Priority: Normal
13, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931
13, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157
==============================End of -
Headers==============================




==============================Original Headers==============================
34, 38 -- From donc-at-asmicro.com Wed May 4 15:13:56 2016
34, 38 -- Received: from resqmta-po-04v.sys.comcast.net (resqmta-po-04v.sys.comcast.net [96.114.154.163])
34, 38 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u44KDuCw013926
34, 38 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 15:13:56 -0500
34, 38 -- Received: from resomta-po-12v.sys.comcast.net ([96.114.154.236])
34, 38 -- by comcast with SMTP
34, 38 -- id y3DqaJDQTIY3My3DwaZ2ZF; Wed, 04 May 2016 20:16:44 +0000
34, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net;
34, 38 -- s=q20140121; t=1462393004;
34, 38 -- bh=+fVVxEgKC0pdxwfk5lTebGJ4zSWiHiQ0f0S4dYDJYXI=;
34, 38 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version:
34, 38 -- Content-Type;
34, 38 -- b=j1TxW0zfo2Y8PQJPALJ8B8esBLefwSxwnc+hGZ2vqw3Auffm1pSO1wfZ+uNK48hnp
34, 38 -- ZtO6uHHUOerwagF2NtkFRv8LdD1Pp5vYeNkPqRUf18ypahIJ1QdWg6YaOoPfsdWn5j
34, 38 -- OV000CFMyo5pdYvEJ5QJSjo/lRJSoG+/ESi7Rdh4nF/nDTLRx0FqtXDa/IS9NS8Qo6
34, 38 -- gK0SO5S24cRuiOXLMz9r2QJmtGgAfYLiNYVpzAlmIlp1R+bH+zrnAvr03OL3C7CUQk
34, 38 -- 7xNL/fTKbJd6EIMd/2iP6/vdDUEHFny/6ZiGmmQLUiBsEXYffhVbfpk9c+hE8g54/D
34, 38 -- 8vPI6+K7dwccg==
34, 38 -- Received: from asm20 ([68.58.83.137])
34, 38 -- by resomta-po-12v.sys.comcast.net with comcast
34, 38 -- id qLGi1s00D2xmMPG01LGi8o; Wed, 04 May 2016 20:16:43 +0000
34, 38 -- Message-ID: {9438297223BD4CAAA9C550D213D915C8-at-asm20}
34, 38 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
34, 38 -- To: "Frederick Schamber" {frederick.schamber-at-gmail.com}
34, 38 -- Cc: "Microscopy List" {microscopy-at-microscopy.com}
34, 38 -- References: {201605041827.u44IRlp4004921-at-ns.microscopy.com} {CAHiSy2Kc7dG58eFBYTmuRmWKXAYE0WWTEkGsp5Mt9X2LHPTEQA-at-mail.gmail.com}
34, 38 -- Subject: Re: how are vacuum pumps rated?
34, 38 -- Date: Wed, 4 May 2016 16:14:17 -0400
34, 38 -- MIME-Version: 1.0
34, 38 -- Content-Type: text/plain;
34, 38 -- format=flowed;
34, 38 -- charset="UTF-8";
34, 38 -- reply-type=original
34, 38 -- Content-Transfer-Encoding: 7bit
34, 38 -- X-Priority: 3
34, 38 -- X-MSMail-Priority: Normal
34, 38 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931
34, 38 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 12:57:53 PM CDT
Subject: [Microscopy] how are vacuum pumps rated?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I want to thank the many people who responded to my initial post about low
vacuum pump.

In reviewing vacuum pump specifications, I note that the flow rate chart
shows a high rate at atmospheric pressure with a rapid decrease in rate as
the input pressure decreases. When this type of pump is specified, is there
general agreement that the flow rate is specified for free air (at 1
atmosphere pressure)?
regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================
----- Original Message -----
From: Don Chernoff at ASM
To: Microscopy List
Sent: Sunday, May 01, 2016 7:01 PM
Subject: low vacuum pump


I'm working with a wafer inspection system that uses vacuum to clamp the
wafer. The manufacturer's specifications call for a modest vacuum:

low vacuum: 150 torr (-24" Hg)

flow rate: { 1 cfm (28 l/m)

Actual flow will be intermittent.



What type of pump would be suitable for this application?

I welcome suggestions for specific models and suggestions for how to
configure the pump.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


==============================Original Headers==============================
13, 36 -- From donc-at-asmicro.com Wed May 4 12:57:52 2016
13, 36 -- Received: from resqmta-ch2-04v.sys.comcast.net (resqmta-ch2-04v.sys.comcast.net [69.252.207.36])
13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u44HvqS3018310
13, 36 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 12:57:52 -0500
13, 36 -- Received: from resomta-ch2-12v.sys.comcast.net ([69.252.207.108])
13, 36 -- by comcast with SMTP
13, 36 -- id y13Ra9nYenIc7y16GaqLzA; Wed, 04 May 2016 18:00:40 +0000
13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net;
13, 36 -- s=q20140121; t=1462384840;
13, 36 -- bh=rLQcAZXBgVIgN7HzeuJJmjrOEuhcJgIo+KezbapneEQ=;
13, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version:
13, 36 -- Content-Type;
13, 36 -- b=EZxq9X3LngRIebRch8mPpB/+EHMSoIQTXQYgInpRqhMn6qoLloFkmxd+bfdLucEdY
13, 36 -- Q7OYd0p+Efi7CAdY9qmvsZG6GF3L3i/uGbRiA/ghjAApdavI27SVkjWek+9vYeMvUr
13, 36 -- I+CaDvaqxFz13X2g9+CK/+7OD25/UYhL8KDzjBoiiuCebz63GcZcK/bOjYXAMBIWD9
13, 36 -- vSou15Qy2M+YK67r0XaIpHTRpStMOolGJm+zBKlPHSp7glHk2ICdHEqKGekaUyCt64
13, 36 -- SxuldlG0YoMaYbNC9HTvYfFWv7Cd1ETZpsr9evQjdkKLAoxusa69b1sDhj7JDScml8
13, 36 -- 8NN+YcII9FAMA==
13, 36 -- Received: from asm20 ([68.58.83.137])
13, 36 -- by resomta-ch2-12v.sys.comcast.net with comcast
13, 36 -- id qJ0f1s00S2xmMPG01J0gSU; Wed, 04 May 2016 18:00:40 +0000
13, 36 -- Message-ID: {15E61CF10A444A239D5D085E7185F339-at-asm20}
13, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
13, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com}
13, 36 -- Subject: how are vacuum pumps rated?
13, 36 -- Date: Wed, 4 May 2016 14:00:41 -0400
13, 36 -- MIME-Version: 1.0
13, 36 -- Content-Type: text/plain;
13, 36 -- format=flowed;
13, 36 -- charset="iso-8859-1";
13, 36 -- reply-type=original
13, 36 -- Content-Transfer-Encoding: 7bit
13, 36 -- X-Priority: 3
13, 36 -- X-MSMail-Priority: Normal
13, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931
13, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 4, 2016 at 5:59:01 AM CDT
Subject: [Microscopy] Cryomicrotomy using glass knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Since years we use diamond knives to cut ultrathin sections of
polymers at low temperature (around -150 *C). When cut the sections
are floating boat/trough integrated on the diamond knife, from where
they are collected.

To cut larger sections we started to make and use glass knives on
which a plastic boat/trough is glued using a "Cavex Set Up Wax", a
dentistry modelling wax, as suggested by the vendor. Unfortunately the
boats fall off at low temperature.

Can anyone suggest a solution or a "glue" that would resist to low temperatures?

Many thanks,
Ondrej


==============================Original Headers==============================
6, 41 -- From ondrej.kotecky-at-gmail.com Wed May 4 05:59:00 2016
6, 41 -- Received: from mail-ig0-f180.google.com (mail-ig0-f180.google.com [209.85.213.180])
6, 41 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u44Ax0kR020028
6, 41 -- for {microscopy-at-microscopy.com} ; Wed, 4 May 2016 05:59:00 -0500
6, 41 -- Received: by mail-ig0-f180.google.com with SMTP id bi2so143580313igb.0
6, 41 -- for {microscopy-at-microscopy.com} ; Wed, 04 May 2016 04:01:47 -0700 (PDT)
6, 41 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 41 -- d=gmail.com; s=20120113;
6, 41 -- h=mime-version:from:date:message-id:subject:to
6, 41 -- :content-transfer-encoding;
6, 41 -- bh=yHiwbV2Nl3yhgTFlabuEWXh+sc1xNAqG4DWxo1ckEHM=;
6, 41 -- b=fR31egbWeNkAcnAQx2R/TkMkG5bPvJ8keX1dwCVPtJ5rpp240JFRXMy4xaSdgJYtYI
6, 41 -- /JQ9PHNJiwGhi7/UR0jxgz0dAl08EWogwWdDtXnmfTfFWh6lxvYdK/TS0OdlEXlR6g91
6, 41 -- MjYphFDqaDPs9d2NIyitBlcgJlNxhGa/i7U9vEfHCArHCEQDubkZTNFij8uc+hkpPQ9C
6, 41 -- Id9tq+BAvvYpxYCaXovETpq1RvqvxEQd9VFHZLJ3CilDn3IQ6x/wigSFR9DgAtwTu66T
6, 41 -- Vo5+q+iWcDllCAZwSfx7QiM3BH2w6sGEbNxdoBymXx7Q0mZGPtUkWTggzyXFpPgmXDGU
6, 41 -- qw3w==
6, 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
6, 41 -- d=1e100.net; s=20130820;
6, 41 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to
6, 41 -- :content-transfer-encoding;
6, 41 -- bh=yHiwbV2Nl3yhgTFlabuEWXh+sc1xNAqG4DWxo1ckEHM=;
6, 41 -- b=K09QTjkxG60A/LJd7Ol014juUHvA102QM1V+5tlBvF7d9z/O83HcYFk12K/UE67iZA
6, 41 -- e+9i+W21EnWxm9mWIGCVsQcgk45kKEP3rnZH8EIBIIuCajkqPIYoIrc4RmP7WoXZX36i
6, 41 -- Kkjz4G4E4MBBEAHM6huqv652y5jk6fRZtA1ogHiu22D+b8FX9AXyQ/eQ/IhOHu0oH3Mt
6, 41 -- 4QCaHHlPNv1/6ZDoAS5n6G+dyBQd0YQNTxArN72MjqY2vRyJCsqdAKqfWeWX6dSHUtKp
6, 41 -- +vOC+Uopx8iSs73L/OJf7M3Tda9srxycYjHr6w5mAno2JjN6CxCvgUUU3SmjHXtVpq5E
6, 41 -- +LRg==
6, 41 -- X-Gm-Message-State: AOPr4FWTFo8zfwP9Pf+8cOGs+ZZobNKpeKOc+pGqU+pxwc1poIQgjKBeWH6blCe1OA+o/H9ZV0pVSkTdXj8bDQ==
6, 41 -- X-Received: by 10.50.10.165 with SMTP id j5mr9842212igb.29.1462359706837; Wed,
6, 41 -- 04 May 2016 04:01:46 -0700 (PDT)
6, 41 -- MIME-Version: 1.0
6, 41 -- Received: by 10.107.26.68 with HTTP; Wed, 4 May 2016 04:01:27 -0700 (PDT)
6, 41 -- From: =?UTF-8?B?T25kxZllaiBLb3RlY2vDvQ==?= {ondrej.kotecky-at-gmail.com}
6, 41 -- Date: Wed, 4 May 2016 13:01:27 +0200
6, 41 -- Message-ID: {CAJRapMBCgjeRwZ2Vm0WRMcGrEaL83kiF+wh4O-yg_rFhHnL2CQ-at-mail.gmail.com}
6, 41 -- Subject: Cryomicrotomy using glass knives
6, 41 -- To: microscopy-at-microscopy.com
6, 41 -- Content-Type: text/plain; charset=UTF-8
6, 41 -- Content-Transfer-Encoding: 8bit
6, 41 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u44Ax0kR020028
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 3, 2016 at 2:10:39 PM CDT
Subject: [Microscopy] Lab Technician Job at the America Museum of Natural History

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The Laboratory Technician will be responsible for assisting and/or training scientific staff and students with Scanning Electron Microscopy, X-ray microanalysis, Laser Scanning Confocal Microscopy, Cathodoluminescence Spectroscopy, X-ray Computed Tomography, specimen preparation and image processing and printing; will maintain functionality and capability of lab equipment; will undergo periodic training on new technologies; will order supplies and track usage and expenditures; will interact with curatorial staff and students on diverse projects using microscopy and digital imaging in the fields of zoology, paleontology, anthropology, archeology, and earth and planetary sciences.

SEM (EDS+EBSD+CL)
Micro-computed tomography
Laser scanning confocal microscopy + Raman spectroscopy
TEM

careers.amnh.org/applicants/Central?quickFind=52033

Henry Towbin
Laboratory Co-manager
Microscopy and Imaging Facility
American Museum of Natural History
79th St & Central Park West
NY, NY 10024



==============================Original Headers==============================
6, 40 -- From htowbin-at-amnh.org Tue May 3 14:10:39 2016
6, 40 -- Received: from spam.amnh.org (mail-mx-001.amnh.org [216.73.244.163])
6, 40 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u43JAcNK012720
6, 40 -- for {Microscopy-at-microscopy.com} ; Tue, 3 May 2016 14:10:38 -0500
6, 40 -- X-ASG-Debug-ID: 1462302802-098fb61b2a2bdb00001-1DjkGe
6, 40 -- Received: from exchange.amnh.org ([172.16.8.237]) by spam.amnh.org with ESMTP id hzjViljB0lS4DocN for {Microscopy-at-microscopy.com} ; Tue, 03 May 2016 15:13:22 -0400 (EDT)
6, 40 -- X-Barracuda-Envelope-From: htowbin-at-amnh.org
6, 40 -- Received: from MAIL-MBX-005.internal.amnh.org ([fe80::f8d9:d817:b12:fce8]) by
6, 40 -- MAIL-CASHT-002.internal.amnh.org ([::1]) with mapi id 14.03.0248.002; Tue, 3
6, 40 -- May 2016 15:13:22 -0400
6, 40 -- From: Henry Towbin {htowbin-at-amnh.org}
6, 40 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
6, 40 -- Subject: Lab Technician Job at the America Museum of Natural History
6, 40 -- Thread-Topic: Lab Technician Job at the America Museum of Natural History
6, 40 -- X-ASG-Orig-Subj: Lab Technician Job at the America Museum of Natural History
6, 40 -- Thread-Index: AQHRpW/bBSyALBCRwkCWYr5+05pI6g==
6, 40 -- Date: Tue, 3 May 2016 19:13:22 +0000
6, 40 -- Message-ID: {1FDA1EB6-EBB1-4334-B90A-50EC09C9FA60-at-amnh.org}
6, 40 -- Accept-Language: en-US
6, 40 -- Content-Language: en-US
6, 40 -- X-MS-Has-Attach:
6, 40 -- X-MS-TNEF-Correlator:
6, 40 -- x-originating-ip: [172.16.8.249]
6, 40 -- Content-Type: text/plain; charset="us-ascii"
6, 40 -- Content-ID: {2B775B42D1B48749BDF7F424722BE887-at-amnh.org}
6, 40 -- MIME-Version: 1.0
6, 40 -- X-Barracuda-Connect: UNKNOWN[172.16.8.237]
6, 40 -- X-Barracuda-Start-Time: 1462302802
6, 40 -- X-Barracuda-URL: https://spam.amnh.org:443/cgi-mod/mark.cgi
6, 40 -- X-Barracuda-Scan-Msg-Size: 1010
6, 40 -- X-Virus-Scanned: by bsmtpd at amnh.org
6, 40 -- X-Barracuda-BRTS-Status: 1
6, 40 -- X-Barracuda-Spam-Score: 0.00
6, 40 -- X-Barracuda-Spam-Status: No, SCORE=0.00 using global scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=4.0 KILL_LEVEL=9.0 tests=
6, 40 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.29269
6, 40 -- Rule breakdown below
6, 40 -- pts rule name description
6, 40 -- ---- ---------------------- --------------------------------------------------
6, 40 -- Content-Transfer-Encoding: 8bit
6, 40 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u43JAcNK012720
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 3, 2016 at 9:37:21 AM CDT
Subject: [Microscopy] [Microscopy}Correction, Low Vac pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Don:
I made a typo in my previous message. The correct model number for the
KNF Mini Diaphragm Pump is N 84.3 ANI.

If you decide to use this pump you will need a base plate to mount it on
and a housing to shield the cooling fan. I can supply drawings for
versions of these items that I'v used previously that are easy and cheap
to make.

You will probably also want some vacuum valves, and for this purpose I
can recommend the mini ball valves (such as Model 4114T11, T13. T64)
sold by McMaster Carr (mcmastercarr.com, 562-692-5911). They also sell a
full line of tubing fittings for polyethylene tubing.

Wil Bigelow

==============================Original Headers==============================
4, 42 -- From bigelow-at-umich.edu Tue May 3 09:37:20 2016
4, 42 -- Received: from mail-wm0-f43.google.com (mail-wm0-f43.google.com [74.125.82.43])
4, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u43EbKuj016160
4, 42 -- for {microscopy-at-microscopy.com} ; Tue, 3 May 2016 09:37:20 -0500
4, 42 -- Received: by mail-wm0-f43.google.com with SMTP id a17so43346876wme.0
4, 42 -- for {microscopy-at-microscopy.com} ; Tue, 03 May 2016 07:40:04 -0700 (PDT)
4, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 42 -- d=umich.edu; s=google;
4, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject
4, 42 -- :content-transfer-encoding;
4, 42 -- bh=s3P39u7sUzezq41+0Cdd4RSU3y7FLKHAYnE1Y2eOeD8=;
4, 42 -- b=YniaPX0DqdyExLahLTBKz+wCF3BtbtqKbFU/bTB0thhQHLnuT2f18hGNr3e6II2jwx
4, 42 -- uBOyPJlyzCNrmoZAr4p6xdyhoUt+kWaP1bZJigYzwdMT1hCxnyuuKj330tcLRalcGp2p
4, 42 -- s/qfgLBfiY0KqUfIkxVAECfZPJibkEEaR5VHM=
4, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
4, 42 -- d=1e100.net; s=20130820;
4, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to
4, 42 -- :subject:content-transfer-encoding;
4, 42 -- bh=s3P39u7sUzezq41+0Cdd4RSU3y7FLKHAYnE1Y2eOeD8=;
4, 42 -- b=HSjAPtW0PLQI043x5FQ2xj3LidBNifOMteuyJww2ONQd/y9wJZwQBEaZLcTPZW4SXn
4, 42 -- PncnTEvVxGn+vcwasdemqNbhWNsY0pnlsyT/rp9COqNoC5elLBdyD2ewEelhe1NkbLnc
4, 42 -- JTMUamJMT+0Lbchtypn9ayG29zVY+q8z1qivgHawZZKcRaK6DjpEtjIcE31xSFTP/dhQ
4, 42 -- Xa8aofLh0ZNXGbgmW+xIy5+ydc7oV8bI6KYJA67qOjKmL+GmSs7LRhDanfy/HVEDAkCl
4, 42 -- MEOLxg5WHHkCUj83qZwSvQoAqcb/8pyX5De+or7SaNM3p/gX9utlRIFnMu7G3klaOe1C
4, 42 -- IOHA==
4, 42 -- X-Gm-Message-State: AOPr4FW3MkNjS+nHdbosHY0XMtuLJMgpD8FAPj5P+1ycG8qrmDVRni9dSsAG6kmT5+wuQib9
4, 42 -- X-Received: by 10.28.181.148 with SMTP id e142mr3694442wmf.38.1462286403026;
4, 42 -- Tue, 03 May 2016 07:40:03 -0700 (PDT)
4, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c])
4, 42 -- by smtp.gmail.com with ESMTPSA id lh1sm4279952wjb.20.2016.05.03.07.40.01
4, 42 -- for {microscopy-at-microscopy.com}
4, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128);
4, 42 -- Tue, 03 May 2016 07:40:02 -0700 (PDT)
4, 42 -- Message-ID: {5728B83F.5090107-at-umich.edu}
4, 42 -- Date: Tue, 03 May 2016 10:39:59 -0400
4, 42 -- From: Wil Bigelow {bigelow-at-umich.edu}
4, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
4, 42 -- MIME-Version: 1.0
4, 42 -- To: microscopy-at-microscopy.com
4, 42 -- Subject: [Microscopy}Correction, Low Vac pump
4, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
4, 42 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 2, 2016 at 9:22:16 PM CDT
Subject: [Microscopy] Low Vacuum Pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I think perhaps the Model N 64.3 ANI Mini Diaphragm Pump manufactured
by KNF Neuberger (www.knf.com/usa.htm) would fill the bill. It will
produce a vacuum below 10 Torr, has a speed of about 4 l//min and costs
less than $600. I've used one of these on a couple of systems I've
designed in the past with very satisfactory results.
Wil Bigelow

==============================Original Headers==============================
1, 42 -- From bigelow-at-umich.edu Mon May 2 21:22:15 2016
1, 42 -- Received: from mail-wm0-f51.google.com (mail-wm0-f51.google.com [74.125.82.51])
1, 42 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u432MECA015382
1, 42 -- for {microscopy-at-microscopy.com} ; Mon, 2 May 2016 21:22:15 -0500
1, 42 -- Received: by mail-wm0-f51.google.com with SMTP id g17so25750067wme.0
1, 42 -- for {microscopy-at-microscopy.com} ; Mon, 02 May 2016 19:24:56 -0700 (PDT)
1, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 42 -- d=umich.edu; s=google;
1, 42 -- h=message-id:date:from:user-agent:mime-version:to:subject
1, 42 -- :content-transfer-encoding;
1, 42 -- bh=JVKGanHbZYxc/0P8cM9WHSxyzk+oEpq4CQXApTw9uPs=;
1, 42 -- b=VFnx0Ac/WjRFyShe2yqugmgSZ1rF2qTWz/rJYbsqL4mei5cdH3Z+JCgq8efelChnh+
1, 42 -- bufGI9XGA5XDn2TDkSk5XqdkdR3wQY5MPLePMUIppS3dnspu3VFRvMa4f0lGvN/wFf6m
1, 42 -- Hw+qjMHQR7rj8I9WCouembRA2tYIx5mjsRv+4=
1, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
1, 42 -- d=1e100.net; s=20130820;
1, 42 -- h=x-gm-message-state:message-id:date:from:user-agent:mime-version:to
1, 42 -- :subject:content-transfer-encoding;
1, 42 -- bh=JVKGanHbZYxc/0P8cM9WHSxyzk+oEpq4CQXApTw9uPs=;
1, 42 -- b=bGFapPthLcKnRh+E3NVxmGaitSj6eNqcDJDo7tiqae9tgzBFJL0/u971gXWILU4+bp
1, 42 -- a0YEkEeqbJw/YiYbIJTJIkNSrRowQeUyKpGNtb79b2kaTLLqW7xtv7LI2EUZzXfnx0f6
1, 42 -- nNn/BiUpRB1rE+1Dl8zYwLdf2IaUoueBfKDuKnlkmEDL+iBQ8pwy2WhAQFiLqNcYd6wV
1, 42 -- +GARn3vUfYsiGGRnkxclQ0JSP7gTWkSAHpVLDSHxQtn3TZCwMcljL/sUx5GwMb41M+Qd
1, 42 -- aWAn9V/XN3a5+tbnDWqGlxNe9XtRkLiy60boDWKQfxLUuUvqE7SMiHsDVejsbOfQ4L6k
1, 42 -- dnQg==
1, 42 -- X-Gm-Message-State: AOPr4FVWpv+K5nkEUb3U+BDQohdidkgMMse7YZI98fylyIszZoQT5QepjQu/Euh3m91JXAju
1, 42 -- X-Received: by 10.194.77.140 with SMTP id s12mr3705105wjw.24.1462242295769;
1, 42 -- Mon, 02 May 2016 19:24:55 -0700 (PDT)
1, 42 -- Received: from Wilbur-Bigelows-G4.local ([2602:306:3b93:aba0:230:65ff:fe6b:155c])
1, 42 -- by smtp.gmail.com with ESMTPSA id d1sm1059056wjb.47.2016.05.02.19.24.54
1, 42 -- for {microscopy-at-microscopy.com}
1, 42 -- (version=TLS1 cipher=ECDHE-RSA-AES128-SHA bits=128/128);
1, 42 -- Mon, 02 May 2016 19:24:55 -0700 (PDT)
1, 42 -- Message-ID: {57280BF4.3010008-at-umich.edu}
1, 42 -- Date: Mon, 02 May 2016 22:24:52 -0400
1, 42 -- From: Wil Bigelow {bigelow-at-umich.edu}
1, 42 -- User-Agent: Mozilla/5.0 (Macintosh; U; PPC Mac OS X 10.4; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
1, 42 -- MIME-Version: 1.0
1, 42 -- To: microscopy-at-microscopy.com
1, 42 -- Subject: [Microscopy]Low Vacuum Pump
1, 42 -- Content-Type: text/plain; charset=ISO-8859-1; format=flowed
1, 42 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 2, 2016 at 2:31:46 AM CDT
Subject: [Microscopy] Re: Photo printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

I haven't had to buy a photo printer for micrographs for some years.
What's the current best? HP doesn't make the Photosmart series anymore,
and the Epson Sure color series specs look good, but ... ?
I need both color and greyscale.
Maybe the Epson P600?
Thanks.

Hi Phil, and colleagues on the list,
we have bought an Officejet Pro 8100 two weeks ago, and we have an Officejet Pro 8000 in use since a few years, directly at a TEM with 2k x 2k camera - to the delight of the users.
no interest in the manufacturing company, of course ...
kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29

Next microscopy conferences:
- 16th Europ Microsc Congress EMC
http://www.emc2016.fr/en/
28 Aug - 2 Sept 2016 in Lyon, FR
- Microscopy Conference 2017
Dreilaendertagung Lausanne, CH
20-25 August 2017
- next Microbiol. conferences:
VAAM/DGHM - Annual Conf
March 5-8, 2017, Wuerzburg




==============================Original Headers==============================
5, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Mon May 2 02:31:45 2016
5, 25 -- Received: from rrzmta2.uni-regensburg.de (rrzmta2.uni-regensburg.de [194.94.155.52])
5, 25 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u427Vjvg031648
5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 02:31:45 -0500
5, 25 -- Received: from rrzmta2.uni-regensburg.de (localhost [127.0.0.1])
5, 25 -- by localhost (Postfix) with SMTP id 2358A45F18
5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 09:34:19 +0200 (CEST)
5, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51])
5, 25 -- by rrzmta2.uni-regensburg.de (Postfix) with ESMTP id 0B95F64F18
5, 25 -- for {Microscopy-at-microscopy.com} ; Mon, 2 May 2016 09:34:18 +0200 (CEST)
5, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de
5, 25 -- with Novell_GroupWise; Mon, 02 May 2016 09:34:21 +0200
5, 25 -- Message-Id: {57271F18020000540005E385-at-gwsmtp1.uni-regensburg.de}
5, 25 -- X-Mailer: Novell GroupWise Internet Agent 14.2.0
5, 25 -- Date: Mon, 02 May 2016 09:34:16 +0200
5, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de}
5, 25 -- To: {oshel1pe-at-cmich.edu} , "microscopy listserver" {Microscopy-at-microscopy.com}
5, 25 -- Subject: Re: [Microscopy] Photo printers
5, 25 -- References: {201604291818.u3TIIUhN013081-at-ns.microscopy.com}
5, 25 -- In-Reply-To: {201604291818.u3TIIUhN013081-at-ns.microscopy.com}
5, 25 -- Mime-Version: 1.0
5, 25 -- Content-Type: text/plain; charset=US-ASCII
5, 25 -- Content-Disposition: inline
5, 25 -- Content-Transfer-Encoding: 8bit
5, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u427Vjvg031648
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 1, 2016 at 6:05:53 PM CDT
Subject: [Microscopy] Clean dry air

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm working with a wafer inspection system that uses clean dry air to float
a vibration isolation table and to release parts from vacuum clamps.

The manufacturer's specifications call for:

Pressure: 80-100 psi

Flow rate: { 1 cfm (28 l/m)

Cleanliness: Filtered to Class I



The actual flow will be intermittent.

I'm looking a quiet system that is easy to maintain. I would like to
receive suggestions of specific equipment configurations.



regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


==============================Original Headers==============================
13, 36 -- From donc-at-asmicro.com Sun May 1 18:05:52 2016
13, 36 -- Received: from resqmta-ch2-05v.sys.comcast.net (resqmta-ch2-05v.sys.comcast.net [69.252.207.37])
13, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u41N5q9g006642
13, 36 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2016 18:05:52 -0500
13, 36 -- Received: from resomta-ch2-18v.sys.comcast.net ([69.252.207.114])
13, 36 -- by comcast with SMTP
13, 36 -- id x0TCaMHkGBE6Ox0TVauH7k; Sun, 01 May 2016 23:08:29 +0000
13, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net;
13, 36 -- s=q20140121; t=1462144109;
13, 36 -- bh=j0dmGOk5NI5qJ+b0IFtRaN3EYlxluzJp3a1o8e70ji0=;
13, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version:
13, 36 -- Content-Type;
13, 36 -- b=I8Ar48oxVMDq1oWmHrD21VlZygSVkr8vLAz8SR1AQb4uuI6/OwgzkAR4NzIY9dt9p
13, 36 -- 5Ozzr6L91kRnt0nzyesLNK5XeQ6A7u3lRV4xzJz0hhMsyFxLETe5MlEyKC7NPWnrcQ
13, 36 -- 6uDpkJQw1nx3q+S4SSgGWQBjG51y+F0I2dmbfhb4sFwC5BK9GB7kP3vNfygW8trENm
13, 36 -- x3Epyc0NSbH8IptlPlj0OY1lz0XbuT/tH5cueg7/1HlbQaQDpBF+1Qrm/0J680Q/rP
13, 36 -- bP9CX+yfforOHy5Eit4vqWwaOlaBc4zOABUHWnb1aUvdyfwqEf6Bk3kKW+q2+BR430
13, 36 -- hz3salvOa83Vg==
13, 36 -- Received: from asm20 ([68.45.155.184])
13, 36 -- by resomta-ch2-18v.sys.comcast.net with comcast
13, 36 -- id pB8V1s0033yyn3Y01B8VdQ; Sun, 01 May 2016 23:08:29 +0000
13, 36 -- Message-ID: {4B176DE84D984487A7C58922082919BD-at-asm20}
13, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
13, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com}
13, 36 -- Subject: Clean dry air
13, 36 -- Date: Sun, 1 May 2016 19:09:08 -0400
13, 36 -- MIME-Version: 1.0
13, 36 -- Content-Type: text/plain;
13, 36 -- format=flowed;
13, 36 -- charset="iso-8859-1";
13, 36 -- reply-type=original
13, 36 -- Content-Transfer-Encoding: 7bit
13, 36 -- X-Priority: 3
13, 36 -- X-MSMail-Priority: Normal
13, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931
13, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: May 1, 2016 at 5:58:19 PM CDT
Subject: [Microscopy] low vacuum pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm working with a wafer inspection system that uses vacuum to clamp the
wafer. The manufacturer's specifications call for a modest vacuum:

low vacuum: 150 torr (-24" Hg)

flow rate: { 1 cfm (28 l/m)

Actual flow will be intermittent.



What type of pump would be suitable for this application?

I welcome suggestions for specific models and suggestions for how to
configure the pump.

regards,
Don
=============================================
Don Chernoff, Ph.D., President
Advanced Surface Microscopy, Inc.; 3250 N. Post Rd., Suite 120;
Indianapolis IN 46226 USA
E-Mail: donc-at-asmicro.com www.asmicro.com
Voice: +1-317-895-5630 Toll free: +1-800-374-8557 (in USA & Canada)
Fax: +1-317-895-5652
[business activities since 1990: analytical services in AFM, AFM probes,
consulting, training, calibration and test specimens,
calibration and measurement software, used NanoScope equipment.]
=============================================


==============================Original Headers==============================
10, 36 -- From donc-at-asmicro.com Sun May 1 17:58:18 2016
10, 36 -- Received: from resqmta-ch2-07v.sys.comcast.net (resqmta-ch2-07v.sys.comcast.net [69.252.207.39])
10, 36 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u41MwITE003272
10, 36 -- for {microscopy-at-microscopy.com} ; Sun, 1 May 2016 17:58:18 -0500
10, 36 -- Received: from resomta-ch2-16v.sys.comcast.net ([69.252.207.112])
10, 36 -- by comcast with SMTP
10, 36 -- id x0M1axB8sp1Bex0MBa2Ax0; Sun, 01 May 2016 23:00:55 +0000
10, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=comcast.net;
10, 36 -- s=q20140121; t=1462143655;
10, 36 -- bh=5YKlv29KfB2X47BOX6fSUaVPqjafQm4cRuCFouKT2o4=;
10, 36 -- h=Received:Received:Message-ID:From:To:Subject:Date:MIME-Version:
10, 36 -- Content-Type;
10, 36 -- b=F4GJAlSZN4QYJMr7WmlCx4WGIS4d5rRo1dsO7Rue/eg6FwvjZ5ciVYBc6uP7IKK2/
10, 36 -- h/M9ZkU/lqd2QcEJmkfeZhVynFNMY6XHSPTFt3Hg1y8+yc/HVnxtpl+XtqA65SNCxe
10, 36 -- E67BUiY1IvBpRbkwKiiZkaJ9kn6wwf+qLyhD1HuJLAwHS9GCzgbgw9lJMzGteOw0/8
10, 36 -- 43kSF1CKYZep+yn5vN5iCquMZJhPlEOOwFptPRHVhrcbAL7SSoLjDHPFG1MkfBkGcJ
10, 36 -- B4tE+4bxaqdp//lBg331TGmlngEZM31der8NUO0pd9RXJ6tzLLY0oKG8bzMOo4UdbO
10, 36 -- 8glqJjYMhmV0g==
10, 36 -- Received: from asm20 ([68.45.155.184])
10, 36 -- by resomta-ch2-16v.sys.comcast.net with comcast
10, 36 -- id pB0u1s00C3yyn3Y01B0u4y; Sun, 01 May 2016 23:00:55 +0000
10, 36 -- Message-ID: {EE952F34508646FA93AD17ECFA9405A1-at-asm20}
10, 36 -- From: "Don Chernoff at ASM" {donc-at-asmicro.com}
10, 36 -- To: "Microscopy List" {microscopy-at-microscopy.com}
10, 36 -- Subject: low vacuum pump
10, 36 -- Date: Sun, 1 May 2016 19:01:31 -0400
10, 36 -- MIME-Version: 1.0
10, 36 -- Content-Type: text/plain;
10, 36 -- format=flowed;
10, 36 -- charset="iso-8859-1";
10, 36 -- reply-type=original
10, 36 -- Content-Transfer-Encoding: 7bit
10, 36 -- X-Priority: 3
10, 36 -- X-MSMail-Priority: Normal
10, 36 -- X-Mailer: Microsoft Outlook Express 6.00.2900.5931
10, 36 -- X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.6157
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 7:05:23 PM CDT
Subject: [Microscopy] viaWWW:Fume hood extension arm

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: rebecca.jackson-at-utsouthwestern.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both rebecca.jackson-at-utsouthwestern.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: rebecca.jackson-at-utsouthwestern.edu
Name: Rebecca Jackson

Organization: UT Southwestern

Title-Subject: [Filtered] Fume hood extension arm

Message: Hey all- We are looking into getting a fume hood extension/extraction arm in our EM lab,
hopefully making it easier and safer for cutting tissues in fix using the dissecting scope. We hope
to do this on the bench with the arm right next to the dish containing the tissue and fix while
using the dissecting scope. Does anyone have experience with these? Could I get some info on what
you have or if these even work well for harsh chemicals? Or do you do/use something totally
different....? Thanks all!!

-Bec.

Login Host: 129.112.109.40
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
13, 52 -- From microscopy.listserver-at-gmail.com Fri Apr 29 19:05:23 2016
13, 52 -- Received: from mail-ig0-f179.google.com (mail-ig0-f179.google.com [209.85.213.179])
13, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3U05NrP025612
13, 52 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 19:05:23 -0500
13, 52 -- Received: by mail-ig0-f179.google.com with SMTP id u10so38003530igr.1
13, 52 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 17:07:53 -0700 (PDT)
13, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 52 -- d=gmail.com; s=20120113;
13, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
13, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
13, 52 -- bh=OY1STbcVf2Pf0/P9bXmp8RUdIsD0lzYcyDpsZeVBPwo=;
13, 52 -- b=F8kQIHgGz+G1bKVzAtpoxucDul6bk3yS9zRjA5vYm8OHt7vyW3B/aVsyoCyYs45Lyx
13, 52 -- I+DqK6eJBTtk44aITr2DTY1ekDlvXGABp0uam2DVoxMidmbW3udZMwYI17nnmTuic7gx
13, 52 -- N9oLE1LsdaP/G7NdoWaQebZgmnZoCxcz+4KkrqDhZ9di0SGWlxywy6O2Ko73izEGvRcz
13, 52 -- HnvG6V40ZmnZzykZAFXW5Qzrtk0/f7FAAZcaK5XQLaPJRZZiSpnFCNno1YQGG4lcaUBg
13, 52 -- E9+/JkKF3/MfgbVxwwu0RfjN4rjUmSoo+DWzNbkhvEmWQYMMDzpKi6QFllTJlv9lekCf
13, 52 -- qkyQ==
13, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
13, 52 -- d=1e100.net; s=20130820;
13, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
13, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
13, 52 -- bh=OY1STbcVf2Pf0/P9bXmp8RUdIsD0lzYcyDpsZeVBPwo=;
13, 52 -- b=b0aCUHuIGtrRirlY+dvXlSGrcOEa+ADouABF5z6s+TjsscL2Rl99bYOc2P7jHpPgAr
13, 52 -- cfcJDxN1wwH2tvMvTGg9yUDneDQxT43mBcpG4xq9s3u59GhGl39FrdT4WDmJHA0b1oy+
13, 52 -- sxTrwA2ouQZfqpiqbH8UpYE5y8ZvG5eh1Uy6u0c/thDRlfEH8VEstnroFtbMKU5WueeB
13, 52 -- NK1s9u5bNmukAIj45WmQmmqZeODRn6HffhrAlCNPa4PTMpkwklJHjvSIyO8EfbZIJA/6
13, 52 -- pNMHH8ZnQf0r8tni0oVu3XCmVR6qli0BxuoSwRafZaI5GBweDJq4Ypr8/rF8sTL1TaCf
13, 52 -- 7Tpw==
13, 52 -- X-Gm-Message-State: AOPr4FUdC9Ysh4KvYmXfsCtqCrkYeM/sXv+u4vN0dlzFc4az7BFmiwo9iBAIZTdqnTCWRg==
13, 52 -- X-Received: by 10.50.128.20 with SMTP id nk20mr2971887igb.86.1461974873232;
13, 52 -- Fri, 29 Apr 2016 17:07:53 -0700 (PDT)
13, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
13, 52 -- by smtp.googlemail.com with ESMTPSA id p1sm11573021iop.12.2016.04.29.17.07.52
13, 52 -- for {microscopy-at-microscopy.com}
13, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
13, 52 -- Fri, 29 Apr 2016 17:07:52 -0700 (PDT)
13, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
13, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
13, 52 -- {microscopylistserver-noreply-at-microscopy.com}
13, 52 -- Subject: viaWWW:Fume hood extension arm
13, 52 -- References: {201604292002.u3TK2pSM005883-at-ns.microscopy.com}
13, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
13, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
13, 52 -- X-Forwarded-Message-Id: {201604292002.u3TK2pSM005883-at-ns.microscopy.com}
13, 52 -- Message-ID: {5723F757.5060803-at-microscopy.com}
13, 52 -- Date: Fri, 29 Apr 2016 19:07:51 -0500
13, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
13, 52 -- Gecko/20100101 Thunderbird/38.7.2
13, 52 -- MIME-Version: 1.0
13, 52 -- In-Reply-To: {201604292002.u3TK2pSM005883-at-ns.microscopy.com}
13, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
13, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 1:56:12 PM CDT
Subject: [Microscopy] Question about soft polymers, toxins, beam sublimation and what

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,


We have a student, that would like to work with a polymer that if 100% polymerized would be ok, but seldom are polymer's 100% polymerized. In other physical states this polymer is toxic.


They hope to make this into a thin film, and use it in x-ray, SEM and TEM.


The toxin/ sensitizer is ethylviologen (ethyl iodide), a bio based substrate known to be a sensitizer, the polymer has the base of Polyvinylbenzylchloride, 4-4-bipyridine, and could include ethylviologen monomers and dipyridyl monomers.




SUGGESTED PRECAUTIONS ALREADY:
Vacuum for a week, mild heat to help gas off, buying own sample stubs and bringing already prepped.
Test just plain polybenzylchloride to watch for sublimation.




Desired instruments of use: SEM, TEM, X-ray




QUESTIONS:


* Even if vacuum is pre-applied for as long as a week in their own lab, will such a product gas out into the TEM causing all the insides to be contaminated?

* Even if vacuum is not a problem, what about sublimation of the polymer by the beam?

* What are your standard precautions for soft polymers, especially those that are toxic? What are the limits of materials you allow in your EM scopes?


Thanks for any help,

Lou Ann







{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu



==============================Original Headers==============================
36, 43 -- From lamiller-at-illinois.edu Fri Apr 29 13:56:11 2016
36, 43 -- Received: from pps03.cites.illinois.edu (pps03.cites.illinois.edu [192.17.82.70])
36, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TIuBKW014296
36, 43 -- for {Microscopy-at-Microscopy.com} ; Fri, 29 Apr 2016 13:56:11 -0500
36, 43 -- Received: from cex1311.ad.uillinois.edu (cex1311.cites.illinois.edu [64.22.177.38])
36, 43 -- by pps03.cites.illinois.edu (8.15.0.59/8.15.0.59) with ESMTPS id u3TIwcbS012397
36, 43 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NOT)
36, 43 -- for {Microscopy-at-Microscopy.com} ; Fri, 29 Apr 2016 13:58:40 -0500
36, 43 -- Received: from cex1311.ad.uillinois.edu (64.22.177.38) by
36, 43 -- cex1311.ad.uillinois.edu (64.22.177.38) with Microsoft SMTP Server (TLS) id
36, 43 -- 15.0.1156.6; Fri, 29 Apr 2016 13:58:39 -0500
36, 43 -- Received: from CHIHT3.ad.uillinois.edu (64.22.177.18) by
36, 43 -- cex1311.ad.uillinois.edu (64.22.177.38) with Microsoft SMTP Server (TLS) id
36, 43 -- 15.0.1156.6 via Frontend Transport; Fri, 29 Apr 2016 13:58:39 -0500
36, 43 -- Received: from CHIMBX2.ad.uillinois.edu ([169.254.7.116]) by
36, 43 -- CHIHT3.ad.uillinois.edu ([64.22.177.75]) with mapi id 14.03.0266.001; Fri, 29
36, 43 -- Apr 2016 13:58:39 -0500
36, 43 -- From: "Miller, Lou A" {lamiller-at-illinois.edu}
36, 43 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com}
36, 43 -- Subject: Question about soft polymers, toxins, beam sublimation and what
36, 43 -- folks allow in their microscopes.
36, 43 -- Thread-Topic: Question about soft polymers, toxins, beam sublimation and
36, 43 -- what folks allow in their microscopes.
36, 43 -- Thread-Index: AQHRokkksmhV19K7HkGf70HOw45FIw==
36, 43 -- Date: Fri, 29 Apr 2016 18:58:39 +0000
36, 43 -- Message-ID: {65950E76-EBAC-44A0-A4FA-E1A296F6BB42-at-illinois.edu}
36, 43 -- Accept-Language: en-US
36, 43 -- Content-Language: en-US
36, 43 -- X-MS-Has-Attach:
36, 43 -- X-MS-TNEF-Correlator:
36, 43 -- x-originating-ip: [128.174.34.198]
36, 43 -- Content-Type: text/plain; charset="utf-8"
36, 43 -- Content-ID: {DE43A6536E566447B9B3D73EA259129C-at-mx.uillinois.edu}
36, 43 -- MIME-Version: 1.0
36, 43 -- X-Spam-Score: 0
36, 43 -- X-Spam-Details: rule=cautious_plus_nq_notspam policy=cautious_plus_nq score=0 spamscore=0
36, 43 -- suspectscore=0 malwarescore=0 phishscore=0 adultscore=0 bulkscore=0
36, 43 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1603290000
36, 43 -- definitions=main-1604290287
36, 43 -- X-Spam-OrigSender: lamiller-at-illinois.edu
36, 43 -- X-Spam-Bar:
36, 43 -- Content-Transfer-Encoding: 8bit
36, 43 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u3TIuBKW014296
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 1:18:00 PM CDT
Subject: [Microscopy] Photo printers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Listers,

I haven't had to buy a photo printer for micrographs for some years.
What's the current best? HP doesn't make the Photosmart series anymore,
and the Epson Sure color series specs look good, but ... ?
I need both color and greyscale.
Maybe the Epson P600?
Thanks.

Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

==============================Original Headers==============================
3, 33 -- From oshel1pe-at-cmich.edu Fri Apr 29 13:17:59 2016
3, 33 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
3, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TIHx48012606
3, 33 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 13:17:59 -0500
3, 33 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged))
3, 33 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3TIKRCS001795
3, 33 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 14:20:27 -0400
3, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by
3, 33 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server
3, 33 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 14:20:28 -0400
3, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by
3, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server
3, 33 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 14:20:27 -0400
3, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu
3, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 29 Apr
3, 33 -- 2016 14:20:27 -0400
3, 33 -- Message-ID: {5723A5EB.8070708-at-cmich.edu}
3, 33 -- Date: Fri, 29 Apr 2016 14:20:27 -0400
3, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
3, 33 -- Reply-To: {oshel1pe-at-cmich.edu}
3, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
3, 33 -- MIME-Version: 1.0
3, 33 -- To: micro {microscopy-at-microscopy.com}
3, 33 -- Subject: Photo printers
3, 33 -- Content-Type: text/plain; charset="ISO-8859-1"; format=flowed
3, 33 -- Content-Transfer-Encoding: 7bit
3, 33 -- X-Originating-IP: [141.209.2.100]
3, 33 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
3, 33 -- X-Spam-Score: -1.70 () [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5)
3, 33 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
3, 33 -- X-CanItPRO-Stream: default
3, 33 -- X-Canit-Stats-ID: 02QMikr4G - e511e4dc73f0 - 20160429
3, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 11:24:15 AM CDT
Subject: [Microscopy] Fwd: LaB6 Update: Could use some suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

LaB6 enthusiasts! This afternoon I am looking at 4 Kimball filaments in
the FIB. Some had a dignified normal death, others an untimely and less
dignified death. What we are going to look at is the elemental
differences between them, from the La right to the base. We are going to
do slice and views and look for defects. Is there anything else you guys
want us to investigate? Let me know and your dreams will come true, we
are the Disneyland of microscopy.

I have been contacted by one of the manufacturers, they too are
interested in the data we get. So things are moving and hopefully we
will be getting good product in the near future.

Next on my agenda will be the quality and performance of FEG tips. How
have your FEG tips been lately?? We at Cornell have tales to tell. Send
me your tales of woe or post them, but lets just say our experience
lately has been similar to our issues with LaB6.

Thanks for interacting, and if we dont keep the suppliers on their
toes, our down time because of poor product will just be expected rather
than a surprise.

jg


==============================Original Headers==============================
7, 30 -- From oshel1pe-at-cmich.edu Fri Apr 29 11:24:14 2016
7, 30 -- Received: from ob4.cmich.edu (ob4.cmich.edu [141.209.20.25])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TGOELT012018
7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 11:24:14 -0500
7, 30 -- Received: from cas.cmich.edu (mail.cmich.edu [141.209.15.40] (may be forged))
7, 30 -- by ob4.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3TGQgkq020469
7, 30 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 12:26:42 -0400
7, 30 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by
7, 30 -- cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) with Microsoft SMTP Server
7, 30 -- (TLS) id 14.3.248.2; Fri, 29 Apr 2016 12:26:43 -0400
7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu
7, 30 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Fri, 29 Apr
7, 30 -- 2016 12:26:42 -0400
7, 30 -- Message-ID: {57238B42.7080700-at-cmich.edu}
7, 30 -- Date: Fri, 29 Apr 2016 12:26:42 -0400
7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
7, 30 -- Reply-To: {oshel1pe-at-cmich.edu}
7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
7, 30 -- MIME-Version: 1.0
7, 30 -- To: micro {microscopy-at-microscopy.com}
7, 30 -- Subject: Fwd: LaB6 Update: Could use some suggestions
7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed
7, 30 -- Content-Transfer-Encoding: 8bit
7, 30 -- X-Originating-IP: [141.209.2.100]
7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
7, 30 -- X-Spam-Score: -0.30 () [Hold at 6.00] RDNS_NONE,_L_LLEXCH,SPF(softfail:0),Bayes(0.0001:-0.5)
7, 30 -- X-CanIt-Geo: ip=141.209.15.40; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
7, 30 -- X-CanItPRO-Stream: default
7, 30 -- X-Canit-Stats-ID: 02QMgqGeS - 8b3b80445ea6 - 20160429
7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.20.25
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 7:33:04 AM CDT
Subject: [Microscopy] viaWWW:Project Scientist Series Electron Microscopy Scientific Staff

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jzheng-at-uci.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jzheng-at-uci.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: jzheng-at-uci.edu
Name: Jian-Guo Zheng

Organization: IMRI UCI

Title-Subject: [Filtered] Project Scientist Series Electron Microscopy Scientific Staff -positions
open at UC Irvine

Message: For details, please visit https://recruit.ap.uci.edu/apply/JPF03381

RECRUITMENT PERIOD
Open April 20th, 2016 through June 30th, 2016
DESCRIPTION

Project Scientist Series -Electron Microscopy Scientific Staff

Salary-Commensurate with experience

POSTING DATE: APRIL 20, 2016 CLOSING DATE: OPEN UNTIL FILLED

DESCRIPTION
Manage the day-to-day operations and help guide the strategic build-out of the new transmission
electron microscopy (TEM) facility at the UC Irvine Materials Research Institute (IRMI). IMRI has a
large and growing user community of over 300 academic and industry users, with advanced materials
characterization instruments that include state-of-the-art TEMs, scanning electron microscopes
(SEMs), focused ion beam (FIB) systems, and a Kratos AXIS Supra surface science instrument (for
details see http://lexi.eng.uci.edu). IMRI has recently built a new TEM facility housing five major
instruments, including four high-end TEMs (Nion UltraSTEM 200 HERMES, JEOL JEM-ARM300CF Grand ARM,
JEM-2800, and JEM-2100F Cryo-TEM) and a dual-beam FIB (Tescan GAIA-3 XMH FIB-SEM). IMRI will be the
first lab in the Americas with the newly introduced JEOL Grand ARM and the Nions high-performance
UltraSTEM 200 HERMES, which provides an energy resolution for electron energy loss spectroscopy
(EELS) of 7 meV or better. Both the Grand ARM and the 2100F Cryo-TEM will be equipped with Gatans
K2 cameras. IMRI is also equipped with a variety of TEM in situ holders and specimen preparation tools.
Two TEM scientific staff positions are available: (1) TEM specialist for materials science
applications and (2) Cryo-TEM specialist for biological and soft materials.
The successful applicant must hold PhD or equivalent degrees in the areas of Materials Science,
Physics, Chemistry or biology, and be TEM experts with at least five years of in-depth, hands-on
experience with TEM/STEM imaging, spectroscopy, TEM sample preparation, and maintenance of TEM
equipment. Applicants who are merely users of TEM will not be considered. The applicant must have
mastery of modern TEM/STEM instrumentation and methods, data collection, analysis, and
interpretation. Candidates with experience operating user facilities and expertise with advanced
TEM/STEM imaging, spectroscopy, and in-situ microscopy techniques are of particular interest. Duties
include: operate, maintain, and improve the TEMs; work with the IMRI leadership to identify and
obtain accessories and additional equipment for a phased build-out of the TEM facility; train,
supervise, and work directly with users to carry out research; co-manage all aspects of day-to-day
IMRI operations; participate in teaching and outreach activities; promote IMRI on and off campus;
secure grants, perform original research, attend scientific conferences, and publish scientific
research in peer-reviewed journals.
The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing
inclusive excellence. All qualified applicants will receive consideration for employment without
regard to race, color, religion, sex, sexual orientation, gender identity, national origin,
disability, age, protected veteran status, or other protected categories covered by the UC
nondiscrimination policy.
TO LEARN MORE AND APPLY
Apply by submitting your application to our online RECRUIT system at:
https://recruit.ap.uci.edu/apply/JPF03381
More information about this recruitment: www.calit2.uci.edu
JOB LOCATION
Irvine, CA
REQUIREMENTS
DOCUMENTS
Curriculum Vitae - Your most recently updated C.V.
Cover Letter
Statement of Research
Statement of Teaching (Optional)
Statement of Contributions to Diversity - Statement addressing how past and/or potential
contributions to diversity will advance UCI's Commitment to Inclusive Excellence.
Misc / Additional (Optional)
REFERENCES
3-5 letters of reference required
HOW TO APPLY
1. Create an ApplicantID
2. Provide required information and documents
3. If any, provide required reference information

To apply these positions, please visit https://recruit.ap.uci.edu/apply/JPF03381
Thanks!

Jian-Guo Zheng, PhD, FRMS
IMRI Director of Facilities
Irvine Materials Research Institute
University Of California, Irvine
3421 Calit2 Building
Irvine, CA 92697-2800
USA
Telephone: 949-824-0441 (office), 949-468-9980 (cell)
Fax: 949-824-8195
Email: jzheng-at-uci.edu


Login Host: 128.195.177.227
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
18, 53 -- From microscopy.listserver-at-gmail.com Fri Apr 29 07:33:04 2016
18, 53 -- Received: from mail-io0-f177.google.com (mail-io0-f177.google.com [209.85.223.177])
18, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TCX4is016899
18, 53 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 07:33:04 -0500
18, 53 -- Received: by mail-io0-f177.google.com with SMTP id u185so122832153iod.3
18, 53 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 05:35:32 -0700 (PDT)
18, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
18, 53 -- d=gmail.com; s=20120113;
18, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
18, 53 -- :mime-version:in-reply-to:content-transfer-encoding;
18, 53 -- bh=p4UtIZcY1K4McGW8oa4sIQvYebtSLfdaHD4bUy8uy5s=;
18, 53 -- b=RxcWg0fMDnWSohAdWnuCVBmQOtxI/2O+64IvVrw5VJfwp5ZbNVV06EoAkjb12mIzZy
18, 53 -- RNvm/dxv1yHuG7z6GWw4jskfUFtrTy57yC1mTEBjuFB2+QNHwTQvAwrfMcrgrliX4Gna
18, 53 -- HltfTCz2wfcIS2LwWqtyS2buWdOaIRmjmmr7MIpRS+GnkA7FqvH5Tw2bqrM+pslUcOD9
18, 53 -- /yX75te2XvIhfZ4eEprgpupa92FoWpqo0Y1Gg3F/pmUeki3t+ZKNoVSDNhY7dtiqpZEP
18, 53 -- ZXQzOK3/UUK7sj1wlO5NYPXhGDmcND5PRrceVEhU0jDiie2q98SK1sYGkKTdnsPe4CKR
18, 53 -- jrLg==
18, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
18, 53 -- d=1e100.net; s=20130820;
18, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
18, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
18, 53 -- bh=p4UtIZcY1K4McGW8oa4sIQvYebtSLfdaHD4bUy8uy5s=;
18, 53 -- b=ceDEDtc1LRWrLLe8usLToHp5WW335tdJz1T3edhk8l+YCW4XsaGHssYanr4wEA36cu
18, 53 -- NawHyhE3mEOjwrgqCAOC7CpzCjg/RccwCl7i2jKge26w17G6EljxlY0pfoY01XB2zwFN
18, 53 -- QnKt6V8QUTaXVsftBzgVTDSfKEoA3w1GvWlJ7p3huAUfh3YBCSUumvgTaUz+rdIb14Ik
18, 53 -- +4tWgrqHw4z1TKupczgskggARNK5EQr7EJ9mdj5h/geiXgMoOpaGv1Ym9HMlBtZ4G3s0
18, 53 -- BHnm6IhYPbfJ6ekmPOA69aTlBZDLGg5LwzqhPGJaf5Tzw6EcPgvlPOdMHtefmx9g3rkI
18, 53 -- DVog==
18, 53 -- X-Gm-Message-State: AOPr4FXhu0z97eERl9mvqapazlxYRFdn3PCFAwKTN9vRzCQM37IVK0vxwLUSBfAwRiVL1Q==
18, 53 -- X-Received: by 10.107.62.197 with SMTP id l188mr12615237ioa.185.1461933332200;
18, 53 -- Fri, 29 Apr 2016 05:35:32 -0700 (PDT)
18, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
18, 53 -- by smtp.googlemail.com with ESMTPSA id n75sm10038584ioe.9.2016.04.29.05.35.31
18, 53 -- for {microscopy-at-microscopy.com}
18, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
18, 53 -- Fri, 29 Apr 2016 05:35:31 -0700 (PDT)
18, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
18, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
18, 53 -- {microscopylistserver-noreply-at-microscopy.com}
18, 53 -- Subject: viaWWW:Project Scientist Series Electron Microscopy Scientific Staff
18, 53 -- -positions open at UC Irvine
18, 53 -- References: {201604282339.u3SNd0np014735-at-ns.microscopy.com}
18, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
18, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
18, 53 -- X-Forwarded-Message-Id: {201604282339.u3SNd0np014735-at-ns.microscopy.com}
18, 53 -- Message-ID: {57235512.4010902-at-microscopy.com}
18, 53 -- Date: Fri, 29 Apr 2016 07:35:30 -0500
18, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
18, 53 -- Gecko/20100101 Thunderbird/38.7.2
18, 53 -- MIME-Version: 1.0
18, 53 -- In-Reply-To: {201604282339.u3SNd0np014735-at-ns.microscopy.com}
18, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed
18, 53 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 29, 2016 at 5:25:00 AM CDT
Subject: [Microscopy] EMS850 CPD use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Gary:

I've already sent you a manual for the EMS 850 CPD but can give you some pointers that are not included in the manual.

What CO2 tank size do you use? Typically your gas supplier will deliver a J-size tank but it MUST have a "Dip tube or Siphon tube" for any CPD to function. The siphon tube draws liquid Co2 from the bottom of the tank, without this you would only draw gas.

What support items do you use? Plastic waste containers to contain Alcohol waste. Small plastic flowmeter to measure the flow of Co2 out of the pressure vessel during the final stage of drying. There are several types of sample holders depending on application, Everything from bulk samples to coverslip holders are in the EMS 850 section of our catalog.

This unit comes with a SST reinforced, 1500psi certified transfer hose. If you didn't get one with the used 850, contact me directly for a replacement.

How automatic is the system? The EMS 850 is semi-automatic. See the details of operation in the manual.

For putting bio specimens into the SEM, how do you factor-in this CPD before going into the chamber? Not sure I understand what the question is. All biological samples must be dried* before coating or insertion into the SEM


Al Coritz (acoritz-at-emsdiasum.com)
Applications & Service Manager
EMS Technical Service

www.emsdiasum.com
800-523-5874
1560 Industry Road
Hatfield, PA 19440


==============================Original Headers==============================
10, 50 -- From acoritz-at-emsdiasum.com Fri Apr 29 05:24:59 2016
10, 50 -- Received: from na01-by2-obe.outbound.protection.outlook.com (mail-by2on0058.outbound.protection.outlook.com [207.46.100.58])
10, 50 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3TAOxwM026474
10, 50 -- for {microscopy-at-microscopy.com} ; Fri, 29 Apr 2016 05:24:59 -0500
10, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
10, 50 -- d=emsdiasum.onmicrosoft.com; s=selector1-emsdiasum-com;
10, 50 -- h=From:To:Date:Subject:Message-ID:Content-Type:MIME-Version;
10, 50 -- bh=MXW7GEVxs1hCnBRvCq+fJw6hhZPJ0QlRZ8iIzCmxXvY=;
10, 50 -- b=ki97grrV1UeIiuyVNXeFK/QwH3PO92cukqIaJs4jGkmVGLqk+oDMJEZmp97kJde+PRaDzMAm/kDb2sgekbgvSjtiGxLbFy34MA4U8t0PTnFt/cO44aZppAlvdbXvwVPjErrwZDmOmiSCSTzLU2L9fmlj/ZAfgRz8ESOKbtioN0Y=
10, 50 -- Received: from BY2PR10MB0822.namprd10.prod.outlook.com (10.163.111.151) by
10, 50 -- BY2PR10MB0821.namprd10.prod.outlook.com (10.163.111.150) with Microsoft SMTP
10, 50 -- Server (TLS) id 15.1.477.8; Fri, 29 Apr 2016 10:27:20 +0000
10, 50 -- Received: from BY2PR10MB0822.namprd10.prod.outlook.com ([10.163.111.151]) by
10, 50 -- BY2PR10MB0822.namprd10.prod.outlook.com ([10.163.111.151]) with mapi id
10, 50 -- 15.01.0477.014; Fri, 29 Apr 2016 10:27:20 +0000
10, 50 -- From: Al Coritz {acoritz-at-emsdiasum.com}
10, 50 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
10, 50 -- Subject: EMS850 CPD use
10, 50 -- Thread-Topic: EMS850 CPD use
10, 50 -- Thread-Index: AdGiAbJePYdUxfgZQwyYIUz6YX53fg==
10, 50 -- Date: Fri, 29 Apr 2016 10:27:20 +0000
10, 50 -- Message-ID: {BY2PR10MB0822FFE5895163DB0041BF90B1660-at-BY2PR10MB0822.namprd10.prod.outlook.com}
10, 50 -- Accept-Language: en-US
10, 50 -- Content-Language: en-US
10, 50 -- X-MS-Has-Attach:
10, 50 -- X-MS-TNEF-Correlator:
10, 50 -- authentication-results: microscopy.com; dkim=none (message not signed)
10, 50 -- header.d=none;microscopy.com; dmarc=none action=none
10, 50 -- header.from=emsdiasum.com;
10, 50 -- x-originating-ip: [50.254.103.2]
10, 50 -- x-ms-office365-filtering-correlation-id: 5cdb143a-d157-4f5e-2af5-08d37018d8a2
10, 50 -- x-microsoft-exchange-diagnostics: 1;BY2PR10MB0821;5:CJGYSkL6DrZaV4SQM3T5kMIaeJ2ZT0AgwczvYOilYxZJ2fsvplsys/MYG1LQeFMacmWmw9okXDKLWlcPYSNoTWOiB8VvkxHQ/QZedfi2OJSHE+2PbP7tLC8+aSPnSKTp8+Tayg8RO7KG4SEL/JObiw==;24:Yq2rdAsC7gG3BcMm0nbrZiOWlsPq2gKjCGGEeL5DSPU1HahkV+GS0BEVzK9k6trMgNMU3XahLRlcNU9tWaFU+IXLEBSVRS0fT+2tO/jZAkY=;7:Zmip0bMQHM4qb+Ofv4B+/eXVsOQBJStqXFRnbNRL2y6cxB1tp5z3d+91K6YNnM217oUcJwR+frjdz5cxo2WcsCLbMS/hMNCXWWOapxVeNhxWBLa4Gq6m3fbqs9+mARi5KgU68MRvc+FjnhNH+No6neOFDZl5psGoMaK0LCez1DABZDSE1ORbGJHl7g1sW0Wv
10, 50 -- x-microsoft-antispam: UriScan:;BCL:0;PCL:0;RULEID:;SRVR:BY2PR10MB0821;
10, 50 -- x-microsoft-antispam-prvs: {BY2PR10MB082139F23946693F9CA8E06FB1660-at-BY2PR10MB0821.namprd10.prod.outlook.com}
10, 50 -- x-exchange-antispam-report-test: UriScan:;
10, 50 -- x-exchange-antispam-report-cfa-test: BCL:0;PCL:0;RULEID:(9101521072)(601004)(2401047)(8121501046)(5005006)(3002001)(10201501046);SRVR:BY2PR10MB0821;BCL:0;PCL:0;RULEID:;SRVR:BY2PR10MB0821;
10, 50 -- x-forefront-prvs: 0927AA37C7
10, 50 -- x-forefront-antispam-report: SFV:NSPM;SFS:(10009020)(6009001)(2900100001)(87936001)(77096005)(3846002)(102836003)(1096002)(1220700001)(6116002)(122556002)(586003)(10400500002)(74316001)(5004730100002)(2351001)(229853001)(99286002)(86362001)(50986999)(2501003)(33656002)(189998001)(5008740100001)(66066001)(110136002)(107886002)(54356999)(5002640100001)(5003600100002)(81166005)(1730700002)(19580395003)(19580405001)(3660700001)(450100001)(76576001)(5640700001)(2906002)(11100500001)(3280700002)(9686002)(15974865002);DIR:OUT;SFP:1101;SCL:1;SRVR:BY2PR10MB0821;H:BY2PR10MB0822.namprd10.prod.outlook.com;FPR:;SPF:None;MLV:sfv;LANG:en;
10, 50 -- spamdiagnosticoutput: 1:23
10, 50 -- spamdiagnosticmetadata: NSPM
10, 50 -- Content-Type: text/plain; charset="us-ascii"
10, 50 -- MIME-Version: 1.0
10, 50 -- X-OriginatorOrg: emsdiasum.com
10, 50 -- X-MS-Exchange-CrossTenant-originalarrivaltime: 29 Apr 2016 10:27:20.3998
10, 50 -- (UTC)
10, 50 -- X-MS-Exchange-CrossTenant-fromentityheader: Hosted
10, 50 -- X-MS-Exchange-CrossTenant-id: 5d139ab6-a6ac-40d7-8ded-e022801e7f6b
10, 50 -- X-MS-Exchange-Transport-CrossTenantHeadersStamped: BY2PR10MB0821
10, 50 -- Content-Transfer-Encoding: 8bit
10, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3TAOxwM026474
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 28, 2016 at 8:45:51 AM CDT
Subject: [Microscopy] Fwd: LaB6 update and some suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists I have a follow up on our LaB6 issue and a
suggestion that should work from one of our microscopy luminaries.

There is correspondence going out to Denka and Kimball regarding our
issues, with our concerns and observations attached, from one of the
major suppliers of microscopy consumables. Hopefully there will be some
movement or at least an acknowledgement that Oh yeah, we did change our
La supplier and welding technique and who we are outsourcing
manufacturing to. Dont think you are the only one having issues guys!
Throw something up on the list and at the least you will get that
reality check that you are not losing your mojo, or the reality check
that indeed you ARE losing your mojo.

Regarding the manual alignment phenomena; if you recall, we align the
Lab6 on the bench as usual, put the gun in the scope, heat it up and the
deflectors cant center the tip, we max out. Take the gun out and the
manual alignment looks great. Henk Colijn theorizes that the resistance
is not the same on the posts so as they heat up the tip gets pulled to
one side or the other. Earl Kirkland suggested that we anneal the
filament before we manually align, which is what we do for our cold
FEGs. I gave it a go with my latest Lab6: ran the HT to 120kV and ran a
dribble current through the crystal over the weekend, getting it good
and hot, manually aligned and so far so good. BUT we should not have to
do this because in the recent past we never had to do this. In our case
it is more downtime which ultimately adds to the cost of the filament.

So guys, I will keep you posted on what happens, any other observations
or suggestions please pass on to our community. Some issues may seem
trivial but we all have a common bond, and our collective mojo is a
pretty awesome force.

jg


==============================Original Headers==============================
7, 30 -- From oshel1pe-at-cmich.edu Thu Apr 28 08:45:50 2016
7, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3SDjoFx009525
7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 08:45:50 -0500
7, 30 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged))
7, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3SDmDod000479
7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 09:48:14 -0400
7, 30 -- Received: from cas1.central.cmich.local (2002:8dd1:f28::8dd1:f28) by
7, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server
7, 30 -- (TLS) id 14.3.248.2; Thu, 28 Apr 2016 09:48:15 -0400
7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu
7, 30 -- (141.209.15.40) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 28 Apr
7, 30 -- 2016 09:48:14 -0400
7, 30 -- Message-ID: {5722149E.7020302-at-cmich.edu}
7, 30 -- Date: Thu, 28 Apr 2016 09:48:14 -0400
7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
7, 30 -- Reply-To: {oshel1pe-at-cmich.edu}
7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
7, 30 -- MIME-Version: 1.0
7, 30 -- To: micro {microscopy-at-microscopy.com}
7, 30 -- Subject: Fwd: LaB6 update and some suggestions
7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed
7, 30 -- Content-Transfer-Encoding: 8bit
7, 30 -- X-Originating-IP: [141.209.2.100]
7, 30 -- X-Bayes-Prob: 0.0001 (Score -0.5, tokens from: -at--at-RPTN, default)
7, 30 -- X-Spam-Score: -1.70 () [Hold at 6.00] L_CTCMUM,L_TMAILSRV,RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.0001:-0.5)
7, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
7, 30 -- X-CanItPRO-Stream: default
7, 30 -- X-Canit-Stats-ID: 05QLNMdBw - f6f14fd4f1be - 20160428
7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 28, 2016 at 7:25:04 AM CDT
Subject: [Microscopy] viaWWW:Current EM Techniques Workshop on May 24 & 25

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: coreimaging-at-umaryland.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both coreimaging-at-umaryland.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: coreimaging-at-umaryland.edu
Name: Ru-ching Hsia

Organization: University of Maryland, Baltimore

Title-Subject: [Filtered] Current EM Techniques Workshop on May 24 & 25

Message: Dear Colleagues,

We would like to remind you that the registration of the UMB Current EM Techniques Workshop
(05/24-05/25) is OPEN. The purpose of this annual workshop is to introduce participants to new
electron microscopy techniques and instruments via live demonstration and open discussion. The
focus of this years workshop is Correlative LM & EM. The workshop will include oral presentations,
Tips-and-Tricks Technology forums on the first day and live instrument demonstration on the second
day. Dr. Lucy Collison of Francis Crick Institute and Richard Schalek of Harvard University will be
the keynote speakers. Demo instruments will feature ATUMtome of RMC-Boeckeler, ASP-1000-IGL of
Microscopy Innovations, Pelco Biowave Pro Microwave System of TedPella, Cryo EM sample preparation
of Leica Microsystems, Correlative Array Tomography of Zeiss, Polyo III, Lynx Tissue Processor of
EMS, Aerosurf Atmospheric SEM of Hitachi/Angstrom.

A joint dinner event with the Chesapeake Microscopy and Microanalysis society (CMMS) on May 24th
provides opportunities for social and scientific exchange among workshop participants and CMMS
members.

More information: http://www.dental.umaryland.edu/2016currentemtechniquesworkshop/

Registrations: https://umbcurrentemtechniqueworkshop.eventbrite.com

Inquiry: mailto:coreimaging-at-umaryland.edu?subject=CMMS%20dinner

We look forward to seeing you in May.


Login Host: 71.244.137.131
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
19, 52 -- From microscopy.listserver-at-gmail.com Thu Apr 28 07:25:03 2016
19, 52 -- Received: from mail-ig0-f175.google.com (mail-ig0-f175.google.com [209.85.213.175])
19, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3SCP3F8018983
19, 52 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 07:25:03 -0500
19, 52 -- Received: by mail-ig0-f175.google.com with SMTP id u10so101840880igr.1
19, 52 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 05:27:28 -0700 (PDT)
19, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
19, 52 -- d=gmail.com; s=20120113;
19, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
19, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
19, 52 -- bh=JfgD0+8MojkY233VATnU/aussmUGB9x9F3LLlCUHPGk=;
19, 52 -- b=DNJ0uLxyBkGCd6sbKX1q3IGlHZ8QhIhiDVHIQn6BusyzXFzi2YjRf1YIK0qGhsorNO
19, 52 -- y9ipF0Cku3zPx9nlbgtsgzHDWxmRH4Ggbnw+4O3wtcfB4TuV3GUwLytmIxxOWy1m0/oK
19, 52 -- k6+5gszk7MhFGQAvD6Z0vu9AX/NEV4oLX/RNabL1PYoZYhBrDrhV7TBsXieXqolLhqbZ
19, 52 -- rVERPRGtym1auUllbcftn0x88WPWlBaQ4Ts7/weD0BxVSsWt7dEe0p3FPykbTuYy7KdI
19, 52 -- /aN96MPqaKZj2dB9oMi+ffD31yi15CYbs2llZ5xNGC4dCg8tSZV+6r8/evRrwYMi82pk
19, 52 -- CRlg==
19, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
19, 52 -- d=1e100.net; s=20130820;
19, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
19, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
19, 52 -- bh=JfgD0+8MojkY233VATnU/aussmUGB9x9F3LLlCUHPGk=;
19, 52 -- b=IUTfJiiUxRjPtHPeb8eYEkW4tEHU6fEdV6tjtC08AJKHd696Yq4/xkIePCCPSMULlL
19, 52 -- cfOWNqdTT5gHmytzfHkJ2AhL61+gSe18Uj7sgSamW6nJYcU/hVJIqkYHWAHY4r2PlvrJ
19, 52 -- NJ/MBuqK76BNStA2kRWWs3cEjiW3k07rVeoYdNbZecuN/hSiXSGeMWO4gInZiNJmeuLX
19, 52 -- 8shutKELTEMErLBfCD+pBLOGdc1D6StQb8jpcrdO0nc9lXsaGOKCGAHMRCWErvEaJu+y
19, 52 -- TKe/lmZUJq22iieBCHtqSY3GEgtxKIHaASwKFcfl0qu/IdedLSRu/S0orIroSlQq1mG3
19, 52 -- bZTw==
19, 52 -- X-Gm-Message-State: AOPr4FUfGO7f3C3I9vdwA/AUKbc3PHrHoZSk/AblhGnyEzFXTmcePXVouVti80Wt8gpAzQ==
19, 52 -- X-Received: by 10.50.50.135 with SMTP id c7mr17306965igo.33.1461846448430;
19, 52 -- Thu, 28 Apr 2016 05:27:28 -0700 (PDT)
19, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
19, 52 -- by smtp.googlemail.com with ESMTPSA id vf17sm17251368igb.22.2016.04.28.05.27.26
19, 52 -- for {microscopy-at-microscopy.com}
19, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
19, 52 -- Thu, 28 Apr 2016 05:27:27 -0700 (PDT)
19, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
19, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
19, 52 -- {microscopylistserver-noreply-at-microscopy.com}
19, 52 -- Subject: viaWWW:Current EM Techniques Workshop on May 24 & 25
19, 52 -- References: {201604280731.u3S7V9xG018726-at-ns.microscopy.com}
19, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
19, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
19, 52 -- X-Forwarded-Message-Id: {201604280731.u3S7V9xG018726-at-ns.microscopy.com}
19, 52 -- Message-ID: {572201AE.50709-at-microscopy.com}
19, 52 -- Date: Thu, 28 Apr 2016 07:27:26 -0500
19, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
19, 52 -- Gecko/20100101 Thunderbird/38.7.2
19, 52 -- MIME-Version: 1.0
19, 52 -- In-Reply-To: {201604280731.u3S7V9xG018726-at-ns.microscopy.com}
19, 52 -- Content-Type: text/plain; charset=UTF-8; format=flowed
19, 52 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 28, 2016 at 2:56:20 AM CDT
Subject: [Microscopy] preparation of fat in mouse liver specimen

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues biologists,

I am preparing mouse livers for TEM the classical way: fixation glutar+Formaldehyde, Osmium post-fixation, embedding in Epon. Some liver samples are loaded with fat, mainly due to a treatment the mice received. The sample were left several weeks in fixative at 4*C before processing so extraction of at least some fat would not be a surprise.

Now on semithin sections stained with methylene blue/Azur II the fat globules have a greenish blue appearance. I thought it was due to the presence of osmium. However, when I observe ultrathin sections of the same samples in TEM, the fat globule are empty, I see nothing, it is only resin inside so they are probably extracted.
My question is: how can the fat be colored in semi-thin sections and empty/extracted in ultrathin section?
How do you explain de greenish blue color with methylene blue/azur II?

Regards,
Stephane


==============================Original Headers==============================
5, 30 -- From nizets2-at-yahoo.com Thu Apr 28 02:56:20 2016
5, 30 -- Received: from nm32-vm7.bullet.mail.bf1.yahoo.com (nm32-vm7.bullet.mail.bf1.yahoo.com [72.30.239.143])
5, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3S7uJFJ019541
5, 30 -- for {microscopy-at-microscopy.com} ; Thu, 28 Apr 2016 02:56:20 -0500
5, 30 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1461830324; bh=JKYEh/Nb7z1jDUPskNBJleAOwbR37AR7SCKdl0LaPa0=; h=Date:From:Reply-To:To:Subject:References:From:Subject; b=c+Tr9DwF6k6g0MSWRJs0oTTwD9VminoLotDyXjl8x8VyOjB60zpdSbGv4HbnnCbuv/FjR1ntzKbpB6yIYL5hlWHSkMCBN0TIhOg48TtKTw5C2vTc6evUUNN1A3P3IVRikTjFhZU019eIMZZBHz+yCc5+tJGbAxwRj5B86NMDX9YoL0Av5blnrLaAfmgujCHIa4GHbBoFiLz/fEXsyBkDV4tCWf8E1UTNUJTdvPWsZ6ryrq2LyM8R7z7lDwYWSQlaNq1aDkMqD6rO6S59sNUH6YPbrPGwYaWKXPQtQUngCi/3dTvjXzEwyYJjXb3byTCmwy/6EOUTsfaGTgiVDuUD7Q==
5, 30 -- Received: from [98.139.214.32] by nm32.bullet.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000
5, 30 -- Received: from [98.139.212.211] by tm15.bullet.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000
5, 30 -- Received: from [127.0.0.1] by omp1020.mail.bf1.yahoo.com with NNFMP; 28 Apr 2016 07:58:44 -0000
5, 30 -- X-Yahoo-Newman-Property: ymail-3
5, 30 -- X-Yahoo-Newman-Id: 33956.30784.bm-at-omp1020.mail.bf1.yahoo.com
5, 30 -- X-YMail-OSG: qXIR1BcVM1m2zt7pjvnoeFCGSPKW_3KeP3rfp5ylNjkSbyAxs3InvvHhp7u7ONR
5, 30 -- 8RsKBerL6w74Ih5C740SC273MEAEOfv4LP_e01qu5WwdnGR8ogSZ6oHFQAJgJkFbXTha_eH8Rs7m
5, 30 -- AxHtg4EI7Pk6kzu5kIg24Ou7op6Es213edNdZOIA54H.Ew8VVwdzkExhbh1t18lKfaxWeUkNdLZI
5, 30 -- Y9mZRezrl8SzpYX1DGeJ_zC3wVz0MdHSHbpaG5U5U5EkRh5lxmpuNjXwJLSUus_Se5IbAw7exYrK
5, 30 -- zFZfI8OYGJAMDXS1oDhA47zwCMwtQXiUv282VJ7tJeKoWTBaGDvMDtAIJjb.fqIt6uEHHK2z0T1R
5, 30 -- X.j2VKFBbaJvQS.OqoPmItEFJCPRk8zocQMtLXV7gVRC_iXeoZOdz6PcgS6Ywf6ARcWCT3zP1bVh
5, 30 -- 93e8RVO7m34gvhnW4LThGZ6vj92gJYvurGyh.Q32ErY9yiVTNVufv.3IdebZ4M.B5FQocnuirzZO
5, 30 -- PD83R
5, 30 -- Received: by 66.196.80.122; Thu, 28 Apr 2016 07:58:43 +0000
5, 30 -- Date: Thu, 28 Apr 2016 07:58:43 +0000 (UTC)
5, 30 -- From: Stephane Nizet {nizets2-at-yahoo.com}
5, 30 -- Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
5, 30 -- To: {microscopy-at-microscopy.com}
5, 30 -- Message-ID: {1594685055.3925265.1461830323263.JavaMail.yahoo-at-mail.yahoo.com}
5, 30 -- Subject: preparation of fat in mouse liver specimen
5, 30 -- MIME-Version: 1.0
5, 30 -- Content-Type: text/plain; charset=UTF-8
5, 30 -- References: {1594685055.3925265.1461830323263.JavaMail.yahoo.ref-at-mail.yahoo.com}
5, 30 -- Content-Transfer-Encoding: 8bit
5, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by ns.microscopy.com id u3S7uJFJ019541
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 27, 2016 at 8:31:09 PM CDT
Subject: [Microscopy] EMS850 CPD use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings to all Listers.

I'm tring to get a used EMS850 CPD up and running and wonder what
others' experiences have been with this unit. This unit is before the
version with a single dial gauge and digital readout.

What CO2 tank size do you use?
What support items do you use?
What hose works for you?
How automatic is the system?

For putting bio specimens into the SEM, how do you factor-in this CPD
before going into the chamber?

All input is appreciated.

TIA,
gary g.

==============================Original Headers==============================
6, 26 -- From gary-at-gaugler.com Wed Apr 27 20:31:08 2016
6, 26 -- Received: from wavecable.net (cgfe-34-tuk.wavecable.net [66.235.59.183])
6, 26 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3S1V8IJ017836
6, 26 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 20:31:08 -0500
6, 26 -- Received: from [172.16.141.11] (HELO wavecable.net)
6, 26 -- by cgfe-3-tuk.wavecable.net (CommuniGate Pro SMTP 6.1.8)
6, 26 -- with ESMTP id 4213441 for microscopy-at-microscopy.com; Wed, 27 Apr 2016 18:26:38 -0700
6, 26 -- X-Junk-Score: 0 []
6, 26 -- X-Cloudmark-Score: 0 []
6, 26 -- X-Cloudmark-Analysis: v=2.1 cv=UdusM/mN c=1 sm=1 tr=0 a=2VnlTcbn8LW/DKys2mm+zQ==:117 a=IkcTkHD0fZMA:10 a=SEOrLOue2igA:10 a=kziv93cY1bsA:10 a=3T1MYC1FAxPeSyEvPIUA:9 a=QEXdDO2ut3YA:10
6, 26 -- Received: from [192.92.0.19] (HELO [127.0.0.1])
6, 26 -- by wavecable.com (CommuniGate Pro SMTP 6.1.8)
6, 26 -- with ESMTP id 76122850 for microscopy-at-microscopy.com; Wed, 27 Apr 2016 18:26:38 -0700
6, 26 -- From: Gary Gaugler {gary-at-gaugler.com}
6, 26 -- Subject: EMS850 CPD use
6, 26 -- Reply-To: gary-at-gaugler.com
6, 26 -- To: microscopy-at-microscopy.com
6, 26 -- Message-ID: {57216861.8000008-at-gaugler.com}
6, 26 -- Date: Wed, 27 Apr 2016 18:33:21 -0700
6, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101
6, 26 -- Thunderbird/38.3.0
6, 26 -- MIME-Version: 1.0
6, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed
6, 26 -- Content-Transfer-Encoding: 7bit
6, 26 -- X-Antivirus: avast! (VPS 160427-0, 04/26/2016), Outbound message
6, 26 -- X-Antivirus-Status: Clean
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 27, 2016 at 4:03:43 PM CDT
Subject: [Microscopy] Field canceling systems in a Talos enclosure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Everyone,
We are planning a field canceling system for the room where we will house an FEI Talos. There is the possibility of building the coils into the microscope enclosure - has anyone done that? Looking to hear experiences.

Thanks



Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



==============================Original Headers==============================
7, 33 -- From gilpin-at-purdue.edu Wed Apr 27 16:03:43 2016
7, 33 -- Received: from mailhub129.itcs.purdue.edu (mailhub129.itcs.purdue.edu [128.210.5.129])
7, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3RL3hZg025450
7, 33 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 16:03:43 -0500
7, 33 -- Received: from exchange.purdue.edu (exchange.purdue.edu [128.210.1.29])
7, 33 -- by mailhub129.itcs.purdue.edu (8.14.4/8.14.4/mta-nopmx.smtp.purdue.edu) with ESMTP id u3RL65n9028670
7, 33 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 17:06:05 -0400
7, 33 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl
7, 33 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1156.6; Wed, 27 Apr
7, 33 -- 2016 17:06:05 -0400
7, 33 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by
7, 33 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id
7, 33 -- 15.00.1156.000; Wed, 27 Apr 2016 17:06:05 -0400
7, 33 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu}
7, 33 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
7, 33 -- Subject: Field canceling systems in a Talos enclosure
7, 33 -- Thread-Topic: Field canceling systems in a Talos enclosure
7, 33 -- Thread-Index: AdGgyF5K4LgsYozqReCcJVLTt1j3RQ==
7, 33 -- Date: Wed, 27 Apr 2016 21:06:04 +0000
7, 33 -- Message-ID: {0d66719e56744cf3bcefdb215588672d-at-wppexc03.purdue.lcl}
7, 33 -- Accept-Language: en-US
7, 33 -- Content-Language: en-US
7, 33 -- X-MS-Has-Attach:
7, 33 -- X-MS-TNEF-Correlator:
7, 33 -- x-ms-exchange-transport-fromentityheader: Hosted
7, 33 -- x-originating-ip: [10.163.23.208]
7, 33 -- Content-Type: text/plain; charset="utf-8"
7, 33 -- MIME-Version: 1.0
7, 33 -- X-PMX-Version: 6.0.2.2308539
7, 33 -- X-PerlMx-URL-Scanned: Yes
7, 33 -- X-PerlMx-Virus-Scanned: Yes
7, 33 -- Content-Transfer-Encoding: 8bit
7, 33 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u3RL3hZg025450
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 27, 2016 at 7:03:35 AM CDT
Subject: [Microscopy] viaWWW: Electron microscopy position open New York University Abu

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: james.weston-at-nyu.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both james.weston-at-nyu.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: james.weston-at-nyu.edu
Name: James Weston

Organization: New York University Abu Dhabi

Title-Subject: [Filtered] Electron microscopy position open

Message: Hello,
We have recently posted a position for a research instrumentation specialist in electron microscopy.

This is a permanent staff position. The individual would be responsible for three electron
microscopes (SEM, TEM, Dual beam).

http://nyuad.nyu.edu/en/about/careers/administration-staff/2016/04/research-instrumentation-specialist---electron-microscopy.html

Please pass on the announcement to colleagues who are not subscribers to this list service

Thanks!
James

Login Host: 91.230.41.193
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
14, 53 -- From microscopy.listserver-at-gmail.com Wed Apr 27 07:03:34 2016
14, 53 -- Received: from mail-ig0-f169.google.com (mail-ig0-f169.google.com [209.85.213.169])
14, 53 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3RC3YaE025396
14, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 07:03:34 -0500
14, 53 -- Received: by mail-ig0-f169.google.com with SMTP id bi2so125402217igb.0
14, 53 -- for {microscopy-at-microscopy.com} ; Wed, 27 Apr 2016 05:05:56 -0700 (PDT)
14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 53 -- d=gmail.com; s=20120113;
14, 53 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
14, 53 -- :mime-version:in-reply-to:content-transfer-encoding;
14, 53 -- bh=3rz6x0y9FZn5UfXpXhayKBQt6T4A4IpZSyScUB+mNH8=;
14, 53 -- b=Oxq+yaC2drOtip+4aT0os6iavp3n4U/45Qt1Im+kXisMAPjDIeVpkD42aF5yGNHj7d
14, 53 -- Kf+tV07ynQvWbqE6iBm6uR7Vlc3fiZtSLa/mrfPFlAwPBKlZiaTj0xPYQ98BYQdlrxBo
14, 53 -- /NmN43IE1qUu7s8ltJrmIohv1oI6oNC4uvN50XESsOkcBoejxnQ6PbSbhyHGpa4UOCur
14, 53 -- xJghYoqwgxzi3yF23/c8QknR8zY/5/dfrhEigv1rwNcmhEz9PHHv1f1LAF7af9PtW/KQ
14, 53 -- 7j8LvO8h5zRTXZW7B/VOjmK/y1WdfegDsPJMvTmELqWPZ49Q8XocHaVbHiDqggd2VAtR
14, 53 -- S+cQ==
14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
14, 53 -- d=1e100.net; s=20130820;
14, 53 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
14, 53 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
14, 53 -- bh=3rz6x0y9FZn5UfXpXhayKBQt6T4A4IpZSyScUB+mNH8=;
14, 53 -- b=euBsABavVKLtHeB3Ow/2gIUEqsKUqk0ejqCVBlMF6umyU6ZTlPzXsJSnAukXVVENk+
14, 53 -- nafGMQjqAkyI/i4ee3uxhLpXm53mARuW7uNF60nr82Yuh61oMWTQkdPxpBEMLJlLvsVR
14, 53 -- cEDTlyuNnP7xZq0+nCAWoSeq+2R/oJIInkwpgQL570HrW6bbzwbZA8ttTDT6IETNpAhR
14, 53 -- NHlOyaiopE59bogOy2LMS4+aali+kT8sRvIdx4rzCU83jjaF7FAAwzkfBIzMczQHNi9a
14, 53 -- DBslCQB2inIp7ElWk+9oTUo06v8X4yRbIULdkR6WSk70hEFuB1XRc6CGIRrwFB9zyQe4
14, 53 -- XSgg==
14, 53 -- X-Gm-Message-State: AOPr4FWSEO/zm8EduKCk9tALamqGbBfJBjPi8HowOImhZhp60mVcSjH0clFfHyVbOTrfLw==
14, 53 -- X-Received: by 10.50.102.207 with SMTP id fq15mr21123589igb.94.1461758755682;
14, 53 -- Wed, 27 Apr 2016 05:05:55 -0700 (PDT)
14, 53 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
14, 53 -- by smtp.googlemail.com with ESMTPSA id u187sm4280912iod.3.2016.04.27.05.05.54
14, 53 -- for {microscopy-at-microscopy.com}
14, 53 -- (version=TLSv1/SSLv3 cipher=OTHER);
14, 53 -- Wed, 27 Apr 2016 05:05:55 -0700 (PDT)
14, 53 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
14, 53 -- X-Google-Original-From: MicroscopyListserver-NoReply
14, 53 -- {microscopylistserver-noreply-at-microscopy.com}
14, 53 -- Subject: viaWWW: Electron microscopy position open New York University Abu
14, 53 -- Dhabi
14, 53 -- References: {201604271002.u3RA29DD023007-at-ns.microscopy.com}
14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
14, 53 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
14, 53 -- X-Forwarded-Message-Id: {201604271002.u3RA29DD023007-at-ns.microscopy.com}
14, 53 -- Message-ID: {5720AB22.20603-at-microscopy.com}
14, 53 -- Date: Wed, 27 Apr 2016 07:05:54 -0500
14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
14, 53 -- Gecko/20100101 Thunderbird/38.7.2
14, 53 -- MIME-Version: 1.0
14, 53 -- In-Reply-To: {201604271002.u3RA29DD023007-at-ns.microscopy.com}
14, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed
14, 53 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 26, 2016 at 9:30:27 PM CDT
Subject: [Microscopy] viaWWW:EELS and EFTEM Workshop at Harvard University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jhyun-at-gatan.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both jhyun-at-gatan.com as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: jhyun-at-gatan.com
Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] EELS and EFTEM Workshop at Harvard University

Message: 10:00 am - 6:00 pm, May 24, 2016
Center for Nanoscale Systems (CNS), Room 303, Laboratory for Integrated Science and Engineering
(LISE) Building, Harvard University, MA

This workshop combines presentations and extensive hands-on training on advanced EELS and EFTEM
techniques and applications in the (S)TEM microscope. Practical experience with EELS is highly
recommended. Participants will learn best practices to set up and optimize their EELS hardware and
experimental protocols so they can capture and extract the maximum amount of compositional and
chemical information from their TEM samples.

Topics include:

-Advanced EELS acquisition techniques extended dynamic range and DualEELS
-Advanced EELS processing techniques, MLLS and NLLS fitting
-Aberration correction
-Quantification using EELS
-Ultrafast compositional mapping
-High energy resolution chemical mapping and identification of different chemical phases
-High energy resolution EELS STEM analysis
-Atomic resolved level analysis using EELS
-How to best optimize the spectrometer and increase the sensitivity
-How to set up the microscope to get the best EELS results

This event is free to registered participants. Register online at:
http://www.gatan.com/company/events/advanced-eels-and-eftem-workshop-harvard-university



Login Host: 50.58.128.11
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
17, 52 -- From microscopy.listserver-at-gmail.com Tue Apr 26 21:30:26 2016
17, 52 -- Received: from mail-io0-f174.google.com (mail-io0-f174.google.com [209.85.223.174])
17, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3R2UQop004230
17, 52 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2016 21:30:26 -0500
17, 52 -- Received: by mail-io0-f174.google.com with SMTP id f89so34292129ioi.0
17, 52 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2016 19:32:46 -0700 (PDT)
17, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 52 -- d=gmail.com; s=20120113;
17, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
17, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
17, 52 -- bh=17RksBSzxB9kPvGaJXx2psgPCEipjwXN+2qugxhW3nQ=;
17, 52 -- b=TWIUBkgpV/TAmrGDsHy+uwJ0TJbBX3bYe7YIpaCb/+CPzlTD/x599lPX9nlavYA9+Y
17, 52 -- P+YR0pi5v2+Ol4Rb2kM+UQaiEK84ISkn7/rh5mRg6ZJvoLrZN0NzdnMNm7CDXkTz2hU2
17, 52 -- uuhFc0fiA+3SIVUUrLRJP2GTol5w4C+3NvoFquktVgGhq86jujcwIjc3wBOXVenBzN7O
17, 52 -- S1SNrUIYBNvwHmVlRM1x5NpC6A3irvC7VX5+Q1MS3O40fI3Mz24gkXElRHbf983TUnA2
17, 52 -- PTCuGMTGg8QVoLJA4oxi3hHeiKBk3zjjbXF4FV42mWH3ZxrWZ0b7dIEQgT+u3OMtoWQb
17, 52 -- eEZw==
17, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 52 -- d=1e100.net; s=20130820;
17, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
17, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
17, 52 -- bh=17RksBSzxB9kPvGaJXx2psgPCEipjwXN+2qugxhW3nQ=;
17, 52 -- b=L6nBZlNS384ylB/lM5MwfeDindzw1wJIWrTxc1wXd9i5cNlMhIwInCgzHXGNl6+1rS
17, 52 -- psIkznYloREC+XWMf2tk1sMMv/GSCKs4kgxSk2W8qA2qeTUtfow+Wc27CmHrAtN1Cy2X
17, 52 -- y2+zP0mqBY+6kgF1xCj22JrL2mz6D5m9grZhOiel3zl/XGvBjQuiHh3BPaRaDzxJyHdP
17, 52 -- 0ykF3ONt7UF3b2ofqZnq3cI5wHKYxzSXS2QutwJ9LIDhc0XmIPnsqj8QfFwF0ykiQg3a
17, 52 -- sjFf05LmRTyOOuPEtLAze0lgzMbD+JiNQWRsUE5toH1kbxIO61b9I6CZqw08ZiDNYt7b
17, 52 -- UFmg==
17, 52 -- X-Gm-Message-State: AOPr4FVr/1m8fbaeFmQ25AuXk+hLJecJJfITObfN2B24p1+Jlus+w8MwwxsQkB2sfM/V0Q==
17, 52 -- X-Received: by 10.107.131.12 with SMTP id f12mr7574132iod.126.1461724366100;
17, 52 -- Tue, 26 Apr 2016 19:32:46 -0700 (PDT)
17, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
17, 52 -- by smtp.googlemail.com with ESMTPSA id jk7sm13388650igb.3.2016.04.26.19.32.45
17, 52 -- for {microscopy-at-microscopy.com}
17, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 52 -- Tue, 26 Apr 2016 19:32:45 -0700 (PDT)
17, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
17, 52 -- {microscopylistserver-noreply-at-microscopy.com}
17, 52 -- Subject: viaWWW:EELS and EFTEM Workshop at Harvard University
17, 52 -- References: {201604252158.u3PLwSAb022985-at-ns.microscopy.com}
17, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
17, 52 -- X-Forwarded-Message-Id: {201604252158.u3PLwSAb022985-at-ns.microscopy.com}
17, 52 -- Message-ID: {572024CD.7090409-at-microscopy.com}
17, 52 -- Date: Tue, 26 Apr 2016 21:32:45 -0500
17, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 52 -- Gecko/20100101 Thunderbird/38.7.2
17, 52 -- MIME-Version: 1.0
17, 52 -- In-Reply-To: {201604252158.u3PLwSAb022985-at-ns.microscopy.com}
17, 52 -- Content-Type: text/plain; charset=UTF-8; format=flowed
17, 52 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 26, 2016 at 9:28:43 PM CDT
Subject: [Microscopy] viaWWW: NIH staff scientist position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: kacharb-at-nidcd.nih.gov

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both kacharb-at-nidcd.nih.gov as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: kacharb-at-nidcd.nih.gov
Name: Bechara Kachar

Organization: NIH

Title-Subject: [Filtered] staff scientist position

Message: Staff Scientist Position

The Laboratory of Cell Structure and Dynamics (LCSD) in the Division of Intramural Research (DIR),
National Institute on Deafness and Other Communication Disorders (NIDCD), National Institutes of
Health (NIH), seeks a Staff Scientist.

The LCSD seeks an integrated molecular understanding of the architecture, dynamics, function, and
renewal of specialized cellular structures underlying the mechanosensory function of auditory and
vestibular sensory hair cells. Current projects at the LCSD involve the use of a combination of cell
biology approaches with super-resolution fluorescence microscopy and cryo-electron microscopy to
examine the molecular architecture of the actin-based sensory stereocilia and their
mechanotransduction molecular complex.

The NIDCD intramural program is comprised of a highly interactive and dynamic group of scientists at
the forefront of research on communication disorders. The LCSD is located in the newly constructed,
multi-disciplinary Porter Neuroscience Research Center on the main NIH campus in Bethesda, Maryland.

The LCSD Staff Scientist serves as a laboratory leader who contributes to mentoring of postdoctoral
fellows and other laboratory staff and trainees. The position requires outstanding interpersonal
skills, teamwork, and excellent oral and written communication abilities. The successful candidate
should have a doctoral degree, postdoctoral research experience, and an outstanding publication
record. Requisite expertise includes molecular and cellular biology, and either advanced
fluorescence microscopy imaging or CryoEM. Salary is commensurate with experience.

Applicants should submit their curriculum vitae with bibliography, statement of interest, and names
and contact information for three references to Bechara Kachar at kacharb-at-nidcd.nih.gov.
Applications will be reviewed starting May 15, 2016, and accepted until the position is filled. HHS,
NIH, and NIDCD are Equal Opportunity Employers.
For more information about the LCSD and NIDCD, please visit our website:
https://www.nidcd.nih.gov/about/staff/bechara-kachar-md
https://www.nidcd.nih.gov/research/labs/section-structural-cell-biology

Login Host: 165.112.75.179
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
17, 52 -- From microscopy.listserver-at-gmail.com Tue Apr 26 21:28:43 2016
17, 52 -- Received: from mail-ig0-f171.google.com (mail-ig0-f171.google.com [209.85.213.171])
17, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3R2ShhL003554
17, 52 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2016 21:28:43 -0500
17, 52 -- Received: by mail-ig0-f171.google.com with SMTP id u10so60479717igr.1
17, 52 -- for {microscopy-at-microscopy.com} ; Tue, 26 Apr 2016 19:31:03 -0700 (PDT)
17, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 52 -- d=gmail.com; s=20120113;
17, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
17, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
17, 52 -- bh=22MxUyjz87AzwVElfcKZ+w2/AKYWRbZOBLgJM6Um4JI=;
17, 52 -- b=dLH2JYjH/1Y+AoM2kclTWjHlqSasVdgKweRI+ssUEu+fNcz4TK/DkfylNgIIURxCJe
17, 52 -- BPVjHWK6cBoSnxF9JrQQharoR0s/Vt8l8jcQ8EBW4JIYxXsAZDGL2qg46DY0JOl1khjh
17, 52 -- vpry6Cs5A5tlceo5OglZZ1O3LdEzgBhSAC01sJ0w4eXrDKOLRvoitfZ7OtwmIcCGpk84
17, 52 -- aQS0Iv1u5Zoz6b76U7OeuKcyTXvp3t4bKRaFt4nss3QCnAvo3Cz2+wnxCQQ9LU84k31q
17, 52 -- axFb1dKlrOGC8umhA8ITk2Czz+9bxOWytGwPmFqHSu8NykpXLLiaU4T8dUVBo9bvsvKU
17, 52 -- PKag==
17, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
17, 52 -- d=1e100.net; s=20130820;
17, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
17, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
17, 52 -- bh=22MxUyjz87AzwVElfcKZ+w2/AKYWRbZOBLgJM6Um4JI=;
17, 52 -- b=OIYJ1tbDPssBXniKoLNcL5mlW0CGxjP0vqR8jCzZi1h3VeW/dr/S1Q4MOYSgNRCxc3
17, 52 -- Rl6PazcZ8BMGt+bgbi8icusL/CEpdpSTB35fSJSO3atJ3euyFURfAPAUS+03dx67prp8
17, 52 -- E89NVhLftGSPyIHcaINhCByRL27gv2SvRaYIX19fJmCc9lv/Aji7rqYZXnLHcBw92QlT
17, 52 -- tGc1k8iEKu2TCImlBGyoUMvAMGOM8GqNwAQJRyKKqtboU1PRXm0yrc5fPG+QcgU59HHr
17, 52 -- 7seZghNzU5WEsyS7IgsQ3rGXv9QAgaktw58JeEK1K9hhOvCrdQVCo19HGPccdJkTMDXB
17, 52 -- PCIg==
17, 52 -- X-Gm-Message-State: AOPr4FW+vdjO3n5Y3JWk7KwhRmjE6BbxC/8ug9mogl/jw81eqBvZse5jHTTCL2eY4mUcjA==
17, 52 -- X-Received: by 10.50.119.67 with SMTP id ks3mr7808961igb.78.1461724262633;
17, 52 -- Tue, 26 Apr 2016 19:31:02 -0700 (PDT)
17, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
17, 52 -- by smtp.googlemail.com with ESMTPSA id ef3sm1476687igb.12.2016.04.26.19.31.01
17, 52 -- for {microscopy-at-microscopy.com}
17, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
17, 52 -- Tue, 26 Apr 2016 19:31:02 -0700 (PDT)
17, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
17, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
17, 52 -- {microscopylistserver-noreply-at-microscopy.com}
17, 52 -- Subject: viaWWW: NIH staff scientist position
17, 52 -- References: {201604252034.u3PKY4WI021002-at-ns.microscopy.com}
17, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
17, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
17, 52 -- X-Forwarded-Message-Id: {201604252034.u3PKY4WI021002-at-ns.microscopy.com}
17, 52 -- Message-ID: {57202465.2050609-at-microscopy.com}
17, 52 -- Date: Tue, 26 Apr 2016 21:31:01 -0500
17, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
17, 52 -- Gecko/20100101 Thunderbird/38.7.2
17, 52 -- MIME-Version: 1.0
17, 52 -- In-Reply-To: {201604252034.u3PKY4WI021002-at-ns.microscopy.com}
17, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
17, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 23, 2016 at 6:41:51 AM CDT
Subject: [Microscopy] viaWWW:SEM Imaging & Processing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: akgadde-at-mix.wvu.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both akgadde-at-mix.wvu.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: akgadde-at-mix.wvu.edu
Name: akshitha

Organization: West Virginia University

Title-Subject: [Filtered] Image Processing

Message: Can someone help me understand in detail how the image processing works in background in a
scanning electron microscope.
How an image is produced from the current signal input to the image processing input in a scanning
electron microscope.

Login Host: 128.163.8.44
Listserver Email Form V - 20120416
---------------------------------------------------------------------------
--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
9, 52 -- From microscopy.listserver-at-gmail.com Sat Apr 23 06:41:50 2016
9, 52 -- Received: from mail-io0-f180.google.com (mail-io0-f180.google.com [209.85.223.180])
9, 52 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3NBfoHX025350
9, 52 -- for {microscopy-at-microscopy.com} ; Sat, 23 Apr 2016 06:41:50 -0500
9, 52 -- Received: by mail-io0-f180.google.com with SMTP id f89so115639775ioi.0
9, 52 -- for {microscopy-at-microscopy.com} ; Sat, 23 Apr 2016 04:43:57 -0700 (PDT)
9, 52 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
9, 52 -- d=gmail.com; s=20120113;
9, 52 -- h=from:subject:references:to:reply-to:message-id:date:user-agent
9, 52 -- :mime-version:in-reply-to:content-transfer-encoding;
9, 52 -- bh=zQN3R+TgrJq8PAk74f+BYdrZ7NuvoDdCUb9DgplLyBk=;
9, 52 -- b=QUTNQrTXr8oS8OOXXxKAFtw8KkoCYwjAPvPuXKhcLAVZ0J3jcp7WtSzWsxVnMYDcbt
9, 52 -- rMGJO/rPRt6Udcm3UzmU8s4JDEwAda+4eq2ruqEeGOZOwooU4ioTl38zIHU1wqlWkGlp
9, 52 -- JfQ90Go+ixYogab/+LW5KdkA1GJwgXl9NJBSSeJvCSd9QMfRbxQ1d8j2NpQ7Hl+UIjuq
9, 52 -- GnJHZ+reKHPBjlHgDSC2tac12hno8smqdETnQkB+W7apjs6yaJOspvz8EnhjcHqdhmxb
9, 52 -- Ru/XtsYvCe4NhpwRG6HbxaQ3I0S+Xf1Sz/Wj52IPvenIevrVpCpqzZ5itGvV3SjBcAzM
9, 52 -- yyYg==
9, 52 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed;
9, 52 -- d=1e100.net; s=20130820;
9, 52 -- h=x-gm-message-state:from:subject:references:to:reply-to:message-id
9, 52 -- :date:user-agent:mime-version:in-reply-to:content-transfer-encoding;
9, 52 -- bh=zQN3R+TgrJq8PAk74f+BYdrZ7NuvoDdCUb9DgplLyBk=;
9, 52 -- b=PGIVtco3pkhEkegGEDj4qKGwkI1y0SfcCk5gByKl5KRO7oil91DYS4fhLnmf9TCrQ8
9, 52 -- zuXIgIq/wmThOeyhHPWrPXfHfoIuSkrKZALZ4b8brkSMpjsbVkBsfW+mKq+lgRazMzCQ
9, 52 -- /KbBls52aLiCp9FThJ0Ofjp60tCIHHO0lrjKA4STd3UNgqhgxgkwerS7f90hQCDvXIqv
9, 52 -- uXGbw0LHKgLxKTMKx09lKpGmV9wDeaFv+l0LZWvXFN+SCvLGssMYqGlKkNb6rwPZAN5J
9, 52 -- jLZBx01HFsnEPSYkaGk2ARxGjaWa2nPBwkDXNYG+NjI7V3I9icpeMTVXJw+i7LXl+dKe
9, 52 -- ISxw==
9, 52 -- X-Gm-Message-State: AOPr4FW+sy4P1/IlXFff+SLO/rnQErepgaSbzQ92Deh5n0aAiBMfMqWPqFpOhQLwE5nN0A==
9, 52 -- X-Received: by 10.107.10.37 with SMTP id u37mr2588648ioi.92.1461411836738;
9, 52 -- Sat, 23 Apr 2016 04:43:56 -0700 (PDT)
9, 52 -- Received: from Nestor-Mac-Pro-ZNL.local ([67.176.143.24])
9, 52 -- by smtp.googlemail.com with ESMTPSA id 90sm6130727ior.21.2016.04.23.04.43.55
9, 52 -- for {microscopy-at-microscopy.com}
9, 52 -- (version=TLSv1/SSLv3 cipher=OTHER);
9, 52 -- Sat, 23 Apr 2016 04:43:56 -0700 (PDT)
9, 52 -- From: MicroscopyListserver-NoReply {microscopy.listserver-at-gmail.com}
9, 52 -- X-Google-Original-From: MicroscopyListserver-NoReply
9, 52 -- {microscopylistserver-noreply-at-microscopy.com}
9, 52 -- Subject: viaWWW:SEM Imaging & Processing
9, 52 -- References: {201604221507.u3MF7c2E018469-at-ns.microscopy.com}
9, 52 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com}
9, 52 -- Reply-To: microscopylistserver-noreply-at-microscopy.com
9, 52 -- X-Forwarded-Message-Id: {201604221507.u3MF7c2E018469-at-ns.microscopy.com}
9, 52 -- Message-ID: {571B5FFB.1080201-at-microscopy.com}
9, 52 -- Date: Sat, 23 Apr 2016 06:43:55 -0500
9, 52 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:38.0)
9, 52 -- Gecko/20100101 Thunderbird/38.7.2
9, 52 -- MIME-Version: 1.0
9, 52 -- In-Reply-To: {201604221507.u3MF7c2E018469-at-ns.microscopy.com}
9, 52 -- Content-Type: text/plain; charset=windows-1252; format=flowed
9, 52 -- Content-Transfer-Encoding: 7bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 2:44:57 PM CDT
Subject: [Microscopy] Attendees, Competitors and Vendors - University of Illinois

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Announcing the Fall Biological Conference at the University of Illinois Materials Research Lab.



DATE: November 2-3, 2016

Conference Title: Diverse Fields of Study... Steps toward Medicine.
________________________________________
An apropos title, as UIUC is starting up its own medical school, MD/PHD with the PhD in Bioengineering.




Attendee registration to open in June and is open to scientists from all over.
=========================================================

Vender registration is open NOW.
==========================


Student and Post Doc Presentation Divisions Competition:
=============================================
up to 4 post docs, up to 9 students
Register from June to October 1st, and upload 1/2 page abstract that will be judged by a panel for acceptance. Winners receive monetary awards, registration waved, and their image on our website for several months.
Students as well as post docs from other institutions are welcome.



Invited Speakers:
==============


Our student competition: up to 4 post docs, up to 9 students.

Dr. Catherine Murphy, Chemistry, MRL associate director
Gold Nanoparticles: Physics, Chemistry, Biology and Ecology

Adele Akhtar of Psionic, The Future of Upper Limb Prosthetic Devices,
Doctoral Candidate Adele Akhtar owns a company that works with prosthetics that can sense touch pressure.

Dr. Ralph Nuzzo - Chemistry/ MRL about Hydrogel and cells

Dr Amy Wagoner - Johnson MechSci Biomaterials, bone implants

Dr Rashid Bashir - Bioengineering.

Dr. Mauro Sardela our new MRL director of the MRL Central Research Facilities, covering what our facility is all about.

Also a representative from our Safety department, topic to be determined.






VENDORS:
===========

This year we have 2 full days of conference,and we have some treats for our vendors. the second day. The vendor area will be open to the public, so this will give you a chance to set up demos and schedule tutorials at your table. Also, you can invite your contacts to come and visit you, regardless of if they have registered. So the second day is public and yours to add visitors, tutorials etc, as you may wish.




The second thing we would like to do for you is give you a chance to speak at the conference. When registering, you can upload a one page abstract to submit, images welcome. This should be in the form of a teaching tutorial. We were able to carve out some time, the 4 best panel judged abstracts will be able to give a 30 minute talk on the second day.

Abstract Deadline for the abstract submission is July 4th, 2016, and folks will know one way or the other by July 10th.

Registration deadline is closer to the meeting, but will end if all tables are full.

There is no fee for the chance to speak, and for 2 full days of tables, the price is $250/ table.

Registration is now open at: "MRL Biological Conference 2016 Exhibitor Registration"
https://my.mrl.illinois.edu/eventreg/register.asp



Thanks! More information at the start of registration for attendees is to come.

Lou Ann


{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Hours: 7am-3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu


==============================Original Headers==============================
47, 43 -- From lamiller-at-illinois.edu Thu Apr 21 14:44:56 2016
47, 43 -- Received: from pps03.cites.illinois.edu (pps03.cites.illinois.edu [192.17.82.70])
47, 43 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3LJiuqk026703
47, 43 -- for {Microscopy-at-Microscopy.com} ; Thu, 21 Apr 2016 14:44:56 -0500
47, 43 -- Received: from cex138.ad.uillinois.edu (cex138.cites.illinois.edu [64.22.177.34])
47, 43 -- by pps03.cites.illinois.edu (8.15.0.59/8.15.0.59) with ESMTPS id u3LJktbq019144
47, 43 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=NOT)
47, 43 -- for {Microscopy-at-Microscopy.com} ; Thu, 21 Apr 2016 14:46:56 -0500
47, 43 -- Received: from cex138.ad.uillinois.edu (64.22.177.34) by
47, 43 -- cex138.ad.uillinois.edu (64.22.177.34) with Microsoft SMTP Server (TLS) id
47, 43 -- 15.0.1156.6; Thu, 21 Apr 2016 14:46:54 -0500
47, 43 -- Received: from CITESHT4.ad.uillinois.edu (192.17.212.154) by
47, 43 -- cex138.ad.uillinois.edu (64.22.177.34) with Microsoft SMTP Server (TLS) id
47, 43 -- 15.0.1156.6 via Frontend Transport; Thu, 21 Apr 2016 14:46:54 -0500
47, 43 -- Received: from CHIMBX2.ad.uillinois.edu ([169.254.7.82]) by
47, 43 -- CITESHT4.ad.uillinois.edu ([192.17.54.147]) with mapi id 14.03.0266.001; Thu,
47, 43 -- 21 Apr 2016 14:46:54 -0500
47, 43 -- From: "Miller, Lou A" {lamiller-at-illinois.edu}
47, 43 -- To: "Microscopy-at-Microscopy.com" {Microscopy-at-Microscopy.com}
47, 43 -- Subject: Attendees, Competitors and Vendors - University of Illinois
47, 43 -- Material Research Lab Fall Conference Nov. 2-3, 2016
47, 43 -- Thread-Topic: Attendees, Competitors and Vendors - University of Illinois
47, 43 -- Material Research Lab Fall Conference Nov. 2-3, 2016
47, 43 -- Thread-Index: AQHRnAaOfevuS1pGa02K+o37DGL4Cg==
47, 43 -- Date: Thu, 21 Apr 2016 19:46:54 +0000
47, 43 -- Message-ID: {642EF0F1-6340-4270-B443-CEDB3643EC79-at-illinois.edu}
47, 43 -- Accept-Language: en-US
47, 43 -- Content-Language: en-US
47, 43 -- X-MS-Has-Attach:
47, 43 -- X-MS-TNEF-Correlator:
47, 43 -- x-originating-ip: [128.174.34.199]
47, 43 -- Content-Type: text/plain; charset="utf-8"
47, 43 -- Content-ID: {66E3A231454207418699DBC2AF64BD39-at-mx.uillinois.edu}
47, 43 -- MIME-Version: 1.0
47, 43 -- X-Spam-Score: 0
47, 43 -- X-Spam-Details: rule=cautious_plus_nq_notspam policy=cautious_plus_nq score=0 spamscore=0
47, 43 -- suspectscore=0 malwarescore=0 phishscore=0 adultscore=0 bulkscore=0
47, 43 -- classifier=spam adjust=0 reason=mlx scancount=1 engine=8.0.1-1603290000
47, 43 -- definitions=main-1604210310
47, 43 -- X-Spam-OrigSender: lamiller-at-illinois.edu
47, 43 -- X-Spam-Bar:
47, 43 -- Content-Transfer-Encoding: 8bit
47, 43 -- X-MIME-Autoconverted: from base64 to 8bit by ns.microscopy.com id u3LJiuqk026703
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 1:09:48 PM CDT
Subject: [Microscopy] Follow up to LaB6 Issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

LaB6 users, I am not losing my touch and there is no LaB6 death vortex
hanging over Ithaca! I have a whole slew of responses that concur with
the issues I have been having. If any of you who are having the same
issue please get in touch with me because if we dont tell the vendors
and the suppliers that their product has changed for the worse they will
still keep selling us garbage because either they dont know or just
dont care. BTW, not one retort from Kimball or Denka
yet.

I think all we want is a LaB6 that shows us a Maltese Cross, doesnt
drift X-Y & Z to the point that the deflectors cant compensate (this is
a common issue, you take the gun out and the tip still looks perfectly
centered, you apply a current and it tilts toward the Emerald City), and
a long life; just the way they used to be ten years ago.

I cant make it to M&M this year or next, thats when the ankle bracelet
comes off, but this may be a good topic for a round table because we the
users are not imagining things.

Send me some more horror stories, I will compile them and post them or
you guys just throw them up on the Microscopy list, do not respond to
Phil, I may owe him a keg if you reply to him.

jg

John L. Grazul {jlg98-at-cornell.edu}


==============================Original Headers==============================
7, 30 -- From oshel1pe-at-cmich.edu Thu Apr 21 13:09:47 2016
7, 30 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
7, 30 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3LI9lt5003974
7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 13:09:47 -0500
7, 30 -- Received: from cas.cmich.edu (async2.cmich.edu [141.209.15.141] (may be forged))
7, 30 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3LIBkX7008383
7, 30 -- for {microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 14:11:47 -0400
7, 30 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by
7, 30 -- cas2.central.cmich.local (2002:8dd1:f8d::8dd1:f8d) with Microsoft SMTP Server
7, 30 -- (TLS) id 14.3.248.2; Thu, 21 Apr 2016 14:11:47 -0400
7, 30 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu
7, 30 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Thu, 21 Apr
7, 30 -- 2016 14:11:46 -0400
7, 30 -- Message-ID: {571917E2.1040407-at-cmich.edu}
7, 30 -- Date: Thu, 21 Apr 2016 14:11:46 -0400
7, 30 -- From: Philip Oshel {oshel1pe-at-cmich.edu}
7, 30 -- Reply-To: {oshel1pe-at-cmich.edu}
7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4
7, 30 -- MIME-Version: 1.0
7, 30 -- To: micro {microscopy-at-microscopy.com}
7, 30 -- Subject: Follow up to LaB6 Issues
7, 30 -- Content-Type: text/plain; charset="windows-1252"; format=flowed
7, 30 -- Content-Transfer-Encoding: 8bit
7, 30 -- X-Originating-IP: [141.209.2.100]
7, 30 -- X-Bayes-Prob: 0.9792 (Score 4, tokens from: -at--at-RPTN, default)
7, 30 -- X-Spam-Score: 2.80 (**) [Hold at 6.00] RDNS_NONE,_L_LEXNRL,_L_LLEXCH,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.9792:4.0)
7, 30 -- X-CanIt-Geo: ip=141.209.15.141; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
7, 30 -- X-CanItPRO-Stream: default
7, 30 -- X-Canit-Stats-ID: 05QJ6bLCS - d89b668dac04 - 20160421
7, 30 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 10:48:08 AM CDT
Subject: [Microscopy] LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We too have had problems with Denka LaB6 on our FEI T20. Short life and bad drift. We have to take the wehnelt out several times to re-center the LaB6 as it runs out of gun tilt. We are experimenting with whether to just turn down the filament current for overnight or if it is best to turn it off completely. After a long spell of just turning it lower we are now turning it on in the morning and off at the end of the day. Its early days but I think we have better lifetime. Next time we change filament we have plans to try a different cathode assembly.


Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility


-----Original Message-----
X-from: oshel1pe-at-cmich.edu [mailto:oshel1pe-at-cmich.edu]
Sent: Wednesday, April 20, 2016 4:19 PM
To: Gilpin, Christopher J {gilpin-at-purdue.edu}

Note! Please do *not* use "Reply"! Send any response to the list or to John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly because anything that comes out of Cornell is un-postable for really good reasons I assume. The huge issue I have been having for the past couple of years is the quality, reliability and life span of my LaB6 sources. Now this is not an issue with just one scope or just one vendor. Tungsten filaments work fantastic and hit all the numbers perfectly in both of our 120kV Tecnais. When we put a LaB6 in really weird things happen. Before you get suspicious and think we just started up with LaB6, this is all that we have ever used on our tools, I just had to test the scopes with W to make sure I wasn't imagining things. We need LaB6 because of the brightness and hours of use; each scope is used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5 years or so, or about 5- 8 filaments, on either of my Tecnais. The engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have flamed out around 500 hours. BTW the vacuum has been rock steady in both scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are talking X-Y and Z. This is to the point that the deflectors can't compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them around my scopes with the same result.

If the darn things didn't cost nearly $900.00 I might tolerate this. I have to give high marks to EMS, they have been listening to my complaints and have made good on the tips that have failed out of the box. But the quality of the product I really have questions about. Has Kimball changed their carbon supplier, is it softer? Why are there cracks? And the last question is have any of you guys had the same or similar issues? And the absolutely final question is am I missing something? After 30+ years of ripping scopes apart I might just have lost the magic touch or there is a vortex of Lab6 instability issues in Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}
--

==============================Original Headers==============================
6, 33 -- From oshel1pe-at-cmich.edu Wed Apr 20 14:58:44 2016 6, 33 -- Received: from ib8.cmich.edu (ib8.cmich.edu [141.209.15.116])
6, 33 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3KJwiex003213
6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 14:58:44 -0500
6, 33 -- Received: from cas.cmich.edu ([141.209.15.90])
6, 33 -- by ib8.cmich.edu (8.14.3/8.14.3/Debian-9.4) with ESMTP id u3KK0dwT018896
6, 33 -- for {microscopy-at-microscopy.com} ; Wed, 20 Apr 2016 16:00:39 -0400
6, 33 -- Received: from cas4.central.cmich.local (2002:8dd1:f03::8dd1:f03) by 6, 33 -- CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from CAS3.central.cmich.local (2002:8dd1:f5a::8dd1:f5a) by 6, 33 -- CAS4.central.cmich.local (2002:8dd1:f03::8dd1:f03) with Microsoft SMTP Server 6, 33 -- (TLS) id 14.3.248.2; Wed, 20 Apr 2016 16:00:39 -0400 6, 33 -- Received: from bio-br024c-mac01.local (141.209.2.100) by mail.cmich.edu 6, 33 -- (141.209.15.90) with Microsoft SMTP Server (TLS) id 14.3.248.2; Wed, 20 Apr 6, 33 -- 2016 16:00:39 -0400 6, 33 -- Message-ID: {5717DFE6.2060309-at-cmich.edu} 6, 33 -- Date: Wed, 20 Apr 2016 16:00:38 -0400 6, 33 -- From: Philip Oshel {oshel1pe-at-cmich.edu} 6, 33 -- Reply-To: {oshel1pe-at-cmich.edu} 6, 33 -- User-Agent: Mozilla/5.0 (Macintosh; U; Intel Mac OS X 10.8; en-US; rv:1.9.1.9) Gecko/20100722 Eudora/3.0.4 6, 33 -- MIME-Version: 1.0 6, 33!

-- To: micro {microscopy-at-microscopy.com} 6, 33 -- Subject: LaB6 Issues anyone?
6, 33 -- Content-Type: text/plain; charset="windows-1252"; format=flowed 6, 33 -- Content-Transfer-Encoding: 8bit 6, 33 -- X-Originating-IP: [141.209.2.100] 6, 33 -- X-Bayes-Prob: 0.9999 (Score 4, tokens from: -at--at-RPTN, default) 6, 33 -- X-Spam-Score: 4.20 (****) [Hold at 6.00] RDNS_NONE,_L_PCM,SPF(softfail:0),RBL(rp-good:-0.1),Bayes(0.9999:4.0)
6, 33 -- X-CanIt-Geo: ip=141.209.15.90; country=US; region=Michigan; city=Mount Pleasant; latitude=43.6147; longitude=-84.7927; http://maps.google.com/maps?q=43.6147,-84.7927&z=6
6, 33 -- X-CanItPRO-Stream: default
6, 33 -- X-Canit-Stats-ID: 05QII0DjR - 7690853b811e - 20160420 6, 33 -- X-Scanned-By: CanIt (www . roaringpenguin . com) on 141.209.15.116 ==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 10:08:03 AM CDT
Subject: [Microscopy] Re: LaB6 Issues anyone?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

John

We to have been having the same problem with Denka LaB6 filaments on a
JEM-3010. We have taken the last two out after aligning them because
they appear to be tilted only to find that they are perfectly aligned!

Over time the last one got better and we got closer to the Maltese cross
. We have not seen a sharp decline in lifetime however.

Regards

Alan

On 4/20/2016 3:00 PM, oshel1pe-at-cmich.edu wrote:
----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Note! Please do *not* use "Reply"! Send any response to the list or to
John L. Grazul {jlg98-at-cornell.edu} . I'm sending this for a colleague
locked behind a Cornell Curtain. Phil

Message:
Fellow microscopists it has been a long time since I have posted mainly
because anything that comes out of Cornell is un-postable for really
good reasons I assume. The huge issue I have been having for the past
couple of years is the quality, reliability and life span of my LaB6
sources. Now this is not an issue with just one scope or just one
vendor. Tungsten filaments work fantastic and hit all the numbers
perfectly in both of our 120kV Tecnais. When we put a LaB6 in really
weird things happen. Before you get suspicious and think we just started
up with LaB6, this is all that we have ever used on our tools, I just
had to test the scopes with W to make sure I wasnt imagining things. We
need LaB6 because of the brightness and hours of use; each scope is used
40-50 hr/week. So much for the introduction, now on to the issues:

1. I have not seen a Maltese cross on either a Kimball or Denka in 5
years or so, or about 5- 8 filaments, on either of my Tecnais. The
engineers and I just undersaturate and go for max brightness.
2. Filament life used to be 2500-3000 hours, the last five tips have
flamed out around 500 hours. BTW the vacuum has been rock steady in both
scopes.
3. I have had two tips fail due to micro cracks in the carbon crucible.
4. The last five tips drift for the first 100-200 hours, and we are
talking X-Y and Z. This is to the point that the deflectors cant
compensate and I have to break vacuum and re-center manually.

5. My 24 Volt power supply for the filament is fine, swapped them around
my scopes with the same result.

If the darn things didnt cost nearly $900.00 I might tolerate this. I
have to give high marks to EMS, they have been listening to my
complaints and have made good on the tips that have failed out of the
box. But the quality of the product I really have questions about. Has
Kimball changed their carbon supplier, is it softer? Why are there
cracks? And the last question is have any of you guys had the same or
similar issues? And the absolutely final question is am I missing
something? After 30+ years of ripping scopes apart I might just have
lost the magic touch or there is a vortex of Lab6 instability issues in
Ithaca.

Please contact me on or off line:
John L. Grazul {jlg98-at-cornell.edu}


--
Alan W Nicholls, PhD
Associate Director, RRC
Director of Research Service Facility (Electron Microscopy)
Research Resources Center - East (M/C 337)
Room 110, Science and Engineering South Building
The University of Illinois at Chicago
845 West Taylor St
Chicago, IL 60607-7058

Tel: 312 996 1227

Web site http://www.rrc.uic.edu/ems
Wiki site https://wiki.rrc.uic.edu/wiki/EMS


==============================Original Headers==============================
11, 21 -- From nicholls-at-uic.edu Thu Apr 21 10:08:03 2016
11, 21 -- Received: from mail-5.cc.uic.edu (mail-5.cc.uic.edu [128.248.156.155])
11, 21 -- by ns.microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id u3LF83DP022100
11, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 10:08:03 -0500
11, 21 -- Received: from [131.193.156.189] (131-193-156-189.pclabs.uic.edu [131.193.156.189])
11, 21 -- (authenticated bits=0)
11, 21 -- by mail-5.cc.uic.edu (8.14.4/8.14.4) with ESMTP id u3LFA2Q1018955
11, 21 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NOT)
11, 21 -- for {Microscopy-at-microscopy.com} ; Thu, 21 Apr 2016 10:10:02 -0500
11, 21 -- Subject: Re: [Microscopy] LaB6 Issues anyone?
11, 21 -- To: Microscopy-at-microscopy.com
11, 21 -- References: {201604202000.u3KK0KVp004130-at-ns.microscopy.com}
11, 21 -- From: Alan Nicholls {nicholls-at-uic.edu}
11, 21 -- Message-ID: {5718EDB7.6030007-at-uic.edu}
11, 21 -- Date: Thu, 21 Apr 2016 10:11:51 -0500
11, 21 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101
11, 21 -- Thunderbird/38.7.2
11, 21 -- MIME-Version: 1.0
11, 21 -- In-Reply-To: {201604202000.u3KK0KVp004130-at-ns.microscopy.com}
11, 21 -- Content-Type: text/plain; charset=windows-1252; format=flowed
11, 21 -- Content-Transfer-Encoding: 8bit
==============================End of - Headers==============================







From: microscopy.listserver-at-gmail.com
Date: April 21, 2016 at 6:13:26 AM CDT
Subject: [Microscopy] viaWWW: Research Fellow (Microscopy of thin film materials) @ Monash

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: matthew.weyland-at-monash.edu

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
---------------------------------------------------------------------------
Remember this posting is most likely not from a Subscriber, so when replying
please copy both matthew.weyland-at-monash.edu as well as the Microscopy Listserver
---------------------------------------------------------------------------

Email: matthew.weyland-at-monash.edu
Name: Matthew Weyland

Organization: Monash University

Title-Subject: [Filtered] Research Fellow (Microscopy of thin film materials)

Message: The following position is currently available:

Research Fellow (Microscopy of thin film materials);

http://www.jobs-monash.jxt.net.au/academic-jobs/research-fellow-microscopy-of-thin-film-materials-/627753

This position is based in both the Monash Centre for Electron Microscopy (MCEM) and The Department
of Materials Science and Engineering at Monash University in Melbourne, Australia. The research is
part of a close collaboration with the School of Materials Science and Engineering, The University
of New South Wales (UNSW), Sydney.

https://platforms.monash.edu/mcem/
http://www.eng.monash.edu.au/materials/
http://www.materials.unsw.edu.au/

Application Closing Date - Thursday 12 May 2016, 11:55pm AEST

For details of the research project, please contact directly.

A/Prof. Matthew Weyland
Monash Centre for Electron Microscopy and Department of Materials Science and Engineering
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia
T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu
mcem.monash.edu


Login Host: 49.127.73.38
Listserver Email Form V - 20120416
---------------------------------------------------------------------------

--
===========================================
Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
18, 52 -- From microscopy.listserver-at-gmail.com Thu Apr 21