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From: microscopy.listserver-at-gmail.com
Date: Sun, 1 Jan 2017 16:27:07 -0600
Subject: [Microscopy] Administrivia: Happy New Year 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2016, the ListServer delivered your messages to more than 4200 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated ~ 171+ Gbits of Email traffic and ~ 2.1 Million
Email messages were sent out this year by my tired and old little server. Down abit
from previous years, but still a steady flow.

This year I will be migrating the Listserver to a new server and ISP configuration so
you might see a short outages during testing and transistions.

As usual you don't want to know how much Junk Mail and spam has been filtered out
far more than you might expect. Apologies to those that have problems with
my filters.


The complete Microscopy ListServer Archives for 2016-1994 (~ are on-line at

http://www.microscopy.com.

A couple of IMPORTANT reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
your Email address from the listserver. The out-of-office / on-vacation
autoreply messages are a real nuisance to posters.

Do not reply to message with the return address of:

MicroscopyListserver-noreply-at-microscopy.com

these are messages forwarded usually from the WWW posting form. They do not
go back to the poster but rather into a black hole, which I rarely check.
If you see a message that has this "No-Reply" return address please post your
reply/comment/answer to:

Microscopy-at-microscopy.com

or if you wish to reply privately, look at the username in the body of
the message the originators Email address is usually listed therein.

As always if you have questions about suitability of postings or
are having problems, feel free to contact me at (zaluzec-at-microscopy.com)

Cheers,

Nestor
Your Friendly Neighborhood SysOp


--
===========================================
Do not reply to this message it is from
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Microscopy-at-Microscopy.com

============================================

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From: zaluzec-at-aaem.amc.anl.gov
Date: Wed, 4 Jan 2017 08:22:42 -0600
Subject: [Microscopy] Testing MListserver Forwarding to select Test File 8:26 AM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

8:26 AM

Does this go to archives now that I have fixed the forwarding system???

===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
Lab: 630-252-7901
Fax: 630-252-4798

iChat: Zaluzec-at-AIM.com
Skype: Zaluzec
Polycom: 146.139.72.119
TPM: http://tpm.amc.anl.gov


Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
=========================================+=====


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==============================End of - Headers==============================




From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Jan 2017 08:41:39 -0600
Subject: [Microscopy] Administrivia: Microscopy Listserver Back on-line

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning Colleagues...

Well my worst nightmares were realized on Monday Night (Jan 2)
when I upgraded my server. Lots of my finely tuned filters
and spam killers "broke". As such the Listserver has been
down for ~ 2 days.

I believe all is fixed now. If you find/identify a problem
please let me know.

Cheers,

Nestor
Your Friendly Neighborhood SysOp



--
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Do not reply to this message it is from
the Microscopy Listserver NO-REPLY forwarding
system. You should send a new message to

Microscopy-at-Microscopy.com

============================================

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Jan 2017 08:44:33 -0600
Subject: [Microscopy] JB-4 microtime manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America


Hi all,

Happy New Year!

Does anyone have a manual for a Sorvall Porter-Blum microtome, Type JB-4? If yes, can you please
send me a copy?

best regards,

Beth Richardson

Georgia Electron Microscopy Lab

University of Georgia

Athens, GA


--
===========================================
Do not reply to this message it is from
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============================================

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Jan 2017 17:48:39 -0600
Subject: [Microscopy] viaWWW:EM facility director position at SDSU

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Email: sbernstein-at-mail.sdsu.edu Name: Sanford Bernstein

Organization: San Diego State University

Title-Subject: EM facility director position at SDSU

Message: Job Announcement: Electron Microscope Facility Lead (Instructional Support Technician III),
College of Sciences - Department of Biology

Job ID:6232; San Diego State University Main Campus; Full-Time Regular

Please note: A completed application is required in order to be considered for this position.

About SDSU: San Diego State University is the oldest and largest higher education institution in the
San Diego region. Since its founding in 1897, SDSU has grown to offer bachelor's degrees in 91
areas, master's degrees in 78 areas and doctorates in 22 areas. SDSU's approximately 35,000
students participate in an academic curriculum distinguished by direct contact with faculty and an
increasing international emphasis that prepares them for a global future.

SDSU is a large, diverse, urban university and Hispanic-Serving Institution with a commitment to
diversity, equity and inclusive excellence. Our campus community is diverse in many ways, including
in terms of race, religion, color, sex, age, disability, marital status, sexual orientation, gender
identity and expression, national origin, pregnancy, medical condition, and covered veteran status.
We strive to build and sustain a welcoming environment for all. SDSU is an equal opportunity employer.
SDSU is seeking applicants with demonstrated experience and/or commitment to teaching and working
effectively with individuals from diverse backgrounds and members of underrepresented groups.

Position Information

Full-time, permanent (probationary) position.
The incumbent is responsible for the day-to-day management of the SDSU Electron Microscope Facility,
oversight of Biology common-use/shared research microscopes (other than EMs), collaborate with and
train users of Biology research microscopes, keep EM Facility and Biology research microscope
equipment funded and operational, order supplies, maintain EM Facility website. The incumbent also
provides input and guidance on the microscopic needs of Biology lab courses.

Salary Range: Anticipated Hiring Salary Range: $4,107 - $6,473 per month (CSU Classification Salary
Range: $4,107 - $6,667 per month). The competitive salary is determined by the education,
experience, and qualifications the candidate brings to the position, internal equity, and the hiring
department's fiscal resources.

Responsibilities:
Training Biology and other SDSU faculty, staff and students in the theory and operation of EM
Facility equipment and other Biology research microscopes
Consulting with Biology and other SDSU faculty and students on research projects using light
microscopy, transmission electron microscopy, and scanning electron microscopy
Oversee external users of the facility and manage the fee-for-service accounting
Collaborating and sharing expertise with Biology faculty and staff to incorporate EM Facility
equipment and other Departmental microscope resources into the Biology curriculum
Maintain equipment associated with EM Facility and Biology research microscopes, coordinate with
repair providers; routine servicing of light microscopes used in biology courses
Acquiring additional instrumentation to enlarge or improve the capabilities of the EM Facility and
Biology research microscopes. This includes submitting grants, or assisting others in the
preparation of grants, to obtain funds for new or updated instrumentation.
Expanding the menu of microscopic techniques the Facility offers, through training and self-study
Maintaining appropriate purchasing procedures, and tracking and billing users of facility
Establishing outreach efforts to area schools and to the community, including designing and
publishing the Facility Web site
Representing the EM Facility and SDSU through service within professional organizations

Knowledge, Skills & Abilities:
Knowledge of the principles and methods related to performing support services.
Knowledge of the principles, information, methods and techniques related to discipline to which
assigned.
Knowledge of the materials and supplies related to the curriculum, their characteristics, and uses.
Ability to plan, organize and schedule work; ability to operate and repair technical and scientific
equipment.
Ability to coordinate support service to meet a comprehensive variety of needs.
Ability to develop off-campus resources related to the discipline for obtaining materials or equipment.
Experience and Education

Equivalent to four years of experience providing instructional support services for a related unit
or discipline, or in producing materials or supplies or repairing equipment in a discipline related
to specialty area to which assigned.

OR

Equivalent to two years of college with 16 semester units in courses involving extensive use of
materials, supplies, or equipment and in a discipline related to the area to which assigned may be
substituted for one year of the required experience.

OR

Equivalent to four years of college with 16 semester units in courses involving extensive use of
materials, supplies, or equipment and in a discipline related to the specialty area to which
assigned may be substituted for two years of the required experience.

Specialized Requirements:
Knowledge of the principles and methods related to light and electron microscopy
Knowledge of the principles, information, methods and techniques related to biological sciences.
Ability to coordinate support service to meet a comprehensive variety of needs of users.
Ability to develop off-campus resources related to the operation, maintenance, and upgrading of
Electron Microscopy Facility equipment Preferred Qualifications

Training/experience in operational design and theory of confocal/epifluorescent microscopy, light
microscopy, scanning and transmission electron microscopy
Experience in preparing samples for confocal/light microscopy, scanning and transmission electron
microscopy
Training/experience operating confocal/light microscopy, scanning and transmission electron microscopy
Experience collecting and processing digital images
Experience purchasing scientific equipment
Relevant Master’s degree
Ph.D. in biological science
Proven abilities in TEM and SEM operations as well as sample preparation techniques for these
microscopes
In addition to knowledge of the principles, information, methods and techniques related to
biological sciences, it is preferable that individual also has knowledge in electron microscopy use
in other scientific and engineering fields.

Application Procedures: Review of applications will begin on Wednesday, January 31, 2017; position
will remain open until filled. The on-line application should be completed in detail. COMPLETION
OF THE ONLINE APPLICATION IS REQUIRED FOR CONSIDERATION, A RESUME ALONE WILL NOT SUFFICE.
Website for applying through the PeopleSoft system:
https://cmsweb.cms.sdsu.edu/psp/HSDPRDF/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL?Page=HRS_CE_HM_PRE&Action=A&SiteId=1

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From: wsalmon-at-wi.mit.edu
Date: Thu, 5 Jan 2017 07:08:12 -0600
Subject: [Microscopy] 2nd Announcement: Analytical and Quantitative Light Microscopy 2017

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

As you start this new year, a reminder that this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA, will run May 3 - May 12, 2017. Applications are due January 27, 2017.

The application is accessible via the course web site is at http://www.mbl.edu/aqlm. Financial assistance is available.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field, confocal microscopes and new emerging technologies.

Laboratory exercises, demonstrations, and discussions include:
• geometrical and physical optics of microscope image formation including Abbe's theory of the microscope and Fourier optics;
• phase contrast, polarization and interference microscopy;
• fluorescence microscopy, quantification of fluorescence, and fluorescent proteins;
• principles and application of digital imaging and quantitative digital image deconvolution;
• digital image processing and object identification and tracking;
• live cell and ratiometric imaging for FRET and ion concentration imaging;
• confocal microscopy and specialized methods such as TIRF and FLIM; and
• new advances in light microscopy such as FCS, PALM, STORM, SIM and Light Sheet.

Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab. It's intense, it's hardcore, and it's really fun.

Why not join us for AQLM (or recommend one of your lab members or core facility users)? You'll never forget it.

Directors: Justin Taraska (National Institutes of Health), Jagesh Shah (Harvard Medical School)
Course Laboratory Director: Wendy Salmon (Whitehead Institute/MIT)

Again, applications due January 27, 2017 and can be access through course web site at http://www.mbl.edu/aqlm.

Cheers,
Jagesh, Justin and Wendy

Visit our Facebook page: http://www.facebook.com/aqlmcourse
Follow us on Twitter: -at-BeadsinMay



Wendy

~~~~~~~~~~~~~~~~~~~~~~~
Wendy Salmon
Light Microscopy Specialist
Whitehead Institute for Biomedical Research
W.M. Keck Imaging Facility
**STREET ADDRESS CHANGE**
455 Main St, Rm 447
Cambridge, MA 02142
c: 617-429-0158
e: wsalmon-at-wi.mit.edu
w: http://staffa.wi.mit.edu/microscopy/


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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Jan 2017 07:53:26 -0600
Subject: [Microscopy] viaWWW: EDS report shows Boron, even its Boron peak is not present in

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Title-Subject: [Filtered] EDS report shows Boron, even its Boron peak is
not present in spectrum.

Message: Hi Listeners,
I am using Oxford EDS (SiLi) on Hitachi S 3500N SEM. Surprisingly, I am
getting false Boron atomic% count.(some times values are in negative) in
spectrum report without any detectable peak in the spectrum. Here I am
using Inca 5.05 I am not able to remove the Boron from the element
count table by confirm elements options because absence of boron peak.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Jan 2017 07:54:24 -0600
Subject: [Microscopy] viaWWW:BSD for Zeiss Supra 40

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Email: gary-at-microtechnics.com Name: Dr Gary Gaugler

Organization: Microtechnics

Title-Subject: [Filtered] BSD for Zeiss Supra 40

Message: Hi All:

Does anyone know of BSD options (scintillator or YAG) for the Zeiss
Supra 40? Robinson has retired. A standard Deben Centaurus does not fit
a chamber port without a large extra cost.

Any ideas or suggestions are appreciated.

gary g.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Jan 2017 22:46:04 -0600
Subject: [Microscopy] viaWWW:EDS report shows Boron, even its Boron peak is not present.

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Email: ravi.thakkar369-at-gmail.com Name: Rav

Title-Subject: [Microscopy] viaWWW: EDS report shows Boron, even its
Boron peak is not present.

Message: I am having boron counts even its absent in confirm elements.
Here, I am using Oxford EDS on Hitachi SEM using Inca 5.05
Hope that image file linked to following link will helpful to you to
understand the issue.

https://www.flickr.com/photos/97321550-at-N08/31998854482/in/dateposted-public/

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From: Unique.VisitsCheap-at-mg-style.cn
Date: 09 Jan 2017 17:49:21 -0800
Subject: re: I need Google Analytics Traffic

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New Year's greetings,

At AgResearch, NZ's Government owned agricultural research institute, we have two microscopy related positions with closing dates in the next few weeks (27th Jan for scientist position, and 20th Jan for PhD scholarship).

These should be of interest to people interested the structure/biology of hair and wool (hard-keratin materials).

The scientist position is aimed at an early career scientist who has completed a PhD and has good team skill with a track record in innovative science.

The PhD scholarship focuses on location of hair-specific proteins within the developing fibre using high-pressure freezing and immunolabelling.

Full details of the two positions can be found on AgResearch's website (www.agresearch.co.nz/careers) and there are also links from AgResearch's Linked In jobs page.

Duane

____________________________
Dr Duane P Harland
Senior Scientist
T +64 3 321 8710
E duane.harland-at-agresearch.co.nz
AgResearch Limited
Lincoln Research Centre
Cnr Springs Rd & Gerald Street, Lincoln
Private Bag 4749 Christchurch 8140, New Zealand
T +64 3 325 9900 F +64 3 325 9946 www.agresearch.co.nz
=======================================================================
Attention: The information contained in this message and/or attachments from AgResearch Limited is intended only for the persons or entities to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipients is prohibited by AgResearch Limited. If you have received this message in error, please notify the sender immediately.
=======================================================================


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From Unique.VisitsCheap-at-mg-style.cn Mon Jan 9 19:44:54 2017
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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Jan 2017 06:46:32 -0600
Subject: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold

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Email: m.aindow-at-uconn.edu Name: Mark Aindow

Organization: University of Connecticut

Title-Subject: [Filtered] Postdoctoral Positions in the UConn-FEI Center for Advanced Microscopy and
Materials Analysis

Message: The University of Connecticut (UConn) has partnered with FEI to establish the Center for
Advanced Microscopy and Materials Analysis (CAMMA), which includes a suite of seven new electron-
and ion-beam instruments. CAMMA will form part of the state-of-the-art Advanced Characterization
Laboratories in the UConn Technology Park, which is currently under construction and will open later
this year. Five of the new instruments are currently housed in the Institute of Materials Science at
UConn and will be relocated to the new Technology Park facility later in 2017.
There are two CAMMA postdoctoral positions available immediately:
1. 3D studies of complex microstructures using Xe plasma FIB. This position will involve the
development and exploitation of serial sectioning and data reconstruction strategies using the FEI
Helios plasma FIB. Candidates should have a good working knowledge of FIB and electron microscopy
techniques, including EDXS and EBSD. A clear understanding of microstructural issues in metallic
alloys, ceramics and coatings is important for this post.
2. Microstructural development in aerospace alloys. This position will involve working with local
aerospace industries on a range of projects relating to microstructural issues in Ni, Ti and Al
alloys, and in thermal barrier coatings. Candidates should have extensive experience with relevant
materials systems and with a broad range of electron microscopy techniques. Due to the nature of
some of the projects, only US citizens or permanent residents will be considered for this second
position.

Both of these positions are for one year in the first instance, with the possibility of renewal. To
apply, please send a resume, together with a full list of publications, a statement of relevant
experience, and contact details for three references to Prof. Mark Aindow (m.aindow-at-uconn.edu).
The University of Connecticut’s commitment to excellence is complemented by our commitment to
building a culturally diverse community. We encourage applications from under-represented groups,
including minorities, women, and people with disabilities. The University of Connecticut is an equal
opportunity employer and program provider. Screening of applications will begin immediately and will
continue until the positions are filled.


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From steven.345345.f-at-gmail.com Tue Jan 10 11:19:33 2017
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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold

Message: How long (in terms of days) in advance can I cut ultrathin sections (usually 70-90 nm) in
preparation for immunogold applications? The resin was medium hardness with catalyst, and I
polymerized it in a 55 degree oven overnight.

I was wondering if the surface of the ultrathin section would be altered over a few days after
cutting and antibodies would be somewhat hindered from access to epitopes.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Jan 2017 18:05:05 -0600
Subject: [Microscopy] viaWWW:Bio TEM Workshop University of Georgia

Contents Retrieved from Microscopy Listserver Archives
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Name:
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School:
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Grade/Education Level:
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Email:
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Bio TEM Workshop -

Message: Biological TEM Workshop

This intensive, three-day workshop will provide a practical and basic theoretical introduction to
the Transmission Electron Microscope and biological sample preparation techniques. Each day will
consist of lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday through Wednesday, March 8-10, 2017, 8am-5pm each day (lunch is provided)

Where: 115 D.E. Brooks Drive, 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login

Deadline: February 27, 2017


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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Jan 2017 18:07:06 -0600
Subject: [Microscopy] viaWWW:Invitation to submit abstracts to the Celtic Sessions at

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ursel.Bangert-at-ul.ie


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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert

Organization: University of Limerick

Title-Subject: [Filtered] Invitation to submit abstracts to the Celtic Sessions at mmc2017

Message: Microcscience Microscopy Congress 2017 – Celtic Sessions

The Microscopy Society of Ireland (MSI) is co-organising two sessions (together with the Scottish
Microscopy Group, SMG) at the Microscience Microscopy Congress (mmc2017), organised by the Royal
Microscopical Society. The event will take place in Manchester, UK, from July 3rd-6th. The
international mmc2017 conference will comprise over 30 symposia, with excellent speakers and vibrant
supporting poster sessions. Further to presenting new research in Ireland and Scotland, the Celtic
Sessions are aimed at showcasing and encouraging collaborative research, and all members of the
international microscopy community are sincerely invited to contribute to the Celtic Sessions, which
will include invited and regular talks, as well as flash presentations and poster sessions. The
abstract submission for mmc2017 is now open. We are inviting abstracts for the Celtic Sessions:
•Biological Processes at the Nanoscale •Inorganic Nanomaterials
For more information about how to participate and submit abstracts, please go to the MSI website
http://microscopy.ie/ or http://microscopy.ie/2017.php, or submit your abstract directly through
the mmc website http://mmc-series.org.uk/about/news/abstract-submission-now-open or through the
official mmc2017 flyer http://microscopy.ie/Doc/MSI_Call_for_Papers_Flyer2.pdf.

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From: conversion.seo-at-gmail.com
Date: 12 Jan 2017 04:30:22 -0800
Subject: re: Boost Sales with Social Media Marketing

Contents Retrieved from Microscopy Listserver Archives
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Hmm...

} Dear sir/madam, For a masters course we have to find the market size for a planar positioning stage. We are having trouble finding sales numbers or estimating how many microscopists use an automatic positioning stage with their microscope or how many would like to use one if the price is right. Hopefully you could provide us with some global estimates. Kind regards, Jacar Brocker (4079450)
}

Global estimates?

Does asking someone else to do the research work for one's degree allow
one to earn it, I wonder?

And a Masters in marketing at that.

Ask A Microscopist may be a reasonable start, though asking around the
University would probably work even better.


Find someone who may use or be interested in product and talk with them.
When you have learned what you and they want to learn, ask if they know
someone else with whom you can talk.
Repeat the above until you have learned enough.


No number of degrees, doctorates or whatever will avoid that. If you
want to succeed at sales, marketing, engineering, science and a number
of other disciplines, you must ignore the knot in your stomach, ignore
the fear of failure, embarrassment or humiliation, get out there and
talk to people. In practice, most of the time there will not be
failure, embarrassment or humiliation. Generally, people will be pleased
you take an interest. For many people, a coffee, croissant and 20
minute break from work makes a nice change, too.

An ex colleague of mine used sometimes to come back from meetings with
potential or actual customers, suppliers, partners or whatever and use
the term "WoFT". If one meets with people in those circumstances it is
for each party to learn, even if what one learns is that nobody wants
the product. IMHO, the only party to whom "WoFT" can apply is oneself.

Gordon.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 16 Jan 2017 17:59:28 -0600
Subject: [Microscopy] viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold

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Organization: Florida State University

Title-Subject: Postdoc position available

Job Title: Post-Doctoral Associate - Electron Microscopist

Summary Description:

Florida State University (FSU) and National High Magnetic Field Laboratory (NHMFL) invite applications for a post-doctoral research associate position to undertake research projects led by Prof. Fumitake Kametani. The research projects aim to understand the nano- and atomic-structural correlations to the material properties by using the advanced electron microscopes including aberration-corrected atomic resolution (scanning) transmission electron microscope (S/TEM) and focused ion beam (FIB). A person who has an experience in the atomic and nano-structural analyses of complicated materials such as high temperature superconductors, carbon nanotube composites, perovskite thin films or energy-related materials is encouraged to apply. This position requires expertise in atomic resolution imaging in a TEM/STEM and nano-fabrication by using a FIB. As listed below, we already have the state-of-art electron microscopes here, so he/she is expected to start working material problems right away.

This position is a full time position with an anticipated starting salary at a minimum of $48,000 and includes health benefits.

Major microscope-related instruments at FSU and NHMFL:
* JEOL ARM200cF Cs-corrected S/TEM with Electron Energy Loss Spectroscopy (EELS) and Energy-dispersive X-ray Spectroscopy (EDS)
* Carl Zeiss 1540EsB field emission scanning electron microscope and FIB equipped with Gas Injection System and OmniProbe in-situ lift-out tool
* Gatan PIPS ion mill
* Micro Support Axis Pro SS ex-situ lift-out tool
* JEOL IB-19500 CP ion cross section polisher

Requirements:
* Ph.D. in materials science, physics, engineering, chemistry, or related field.
* Must have experience to operate TEM.
* Must have experience preparing specimens using FIB.
* Preferred to have experience operating Cs-corrected STEMs.
* Preferred to have experience in EELS.

Please directly send CV including 2 references to:

Fumitake Kametani
Associate Professor
Department of Mechanical Engineering, Florida State University,
and Applied Superconductivity Center, National High Magnetic Field Laboratory
2031 E. Paul Dirac Dr., Tallahassee, FL 32310
Tel: (850) 645-7491
E-mail: fkametani-at-fsu.edu


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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Eye Research Institute

Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold

Message: Hello,
Second Request - Not sure if the first message was transmitted...
What is the maximum time I need to wait in order to apply immunogold reagents (buffers, etc.) to
ultrathin sections of LR White blocks (Medium hardness with catalyst?
Thanks much,
Vickie

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From: leunissen-at-aurion.nl
Date: Mon, 16 Jan 2017 18:20:18 -0600
Subject: [Microscopy] Re: viaWWW:LR White Ultrathin Sections Prepared for ImmunoGold

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good day Vickie,

I do not think there is such a thing as a maximum time you have to wait before which LR white sections would be suited for immuno. Perhaps you are wondering if there is a minimum time before sections are suited? As far as our experience goes LR-white and Lowicryl sections seem to keep pretty much indefinitely. Perhaps it would be wise if they were kept in a dessicator. I do not think there is a minimum time one should consider having to wait. If things change post-sectioning I would expect epitopes to change (oxidize?) and that hardly ever is beneficial. And how would ‘buffers etc’ relate to that, I do not understand?

Are you getting results, or not getting results, that make you wonder whether section age plays a role?

If you like, please feel free to get in touch off-list. I will be happy to help.

Jan Leunissen
- - -
Aurion - ImmunoGold Reagents
www.aurion.nl





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From: tina-at-pbrc.hawaii.edu
Date: Mon, 16 Jan 2017 19:27:16 -0600
Subject: [Microscopy] Re: viaWWW:LR White Ultrathin Sections Prepared for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi, Vickie-

I'm sure it all depends on the epitope.

Here's a story: Someone once had LR-White sections that were more than two
years old, from another institution. They went ahead and applied their
antibody, secondary antibody and gold, and seemed to have every expectation
that it would work (whether based on experience or blind faith, I do not know).
However, they needed images immediately for a grant proposal, or at least they
needed to know if a particular protein was on a particular organ in the squid.
My TEM (the only one in the state) was down. In a panic, we stuck them in the
SEM wehere we could see the gold particles on the surface of the section, and
we could also see outlines of the ultrastructure because parts of the tissue
had bulged out of the resin enough to give some relief. In fact, it looked
really cool. And they were able to state that the protein was in that organ. If
you've ever sectioned a block and then let it sit for some time (hours,
years?), and than sectioned again you've probably noticed that some tissues
expand out further than others over time.

Anyway, in this case I'm sure their antibody would have worked right away on
freshly-cut sections as well as on sections that had been sitting on grids for
more than two years. The resin may flow somewhat over time. Your mileage
may vary. Perform a bunch of timing experiments and report back!

Aloha,
Tina

} Email: vakimler-at-oakland.edu Name: Vickie Kimler
}
} Organization: Eye Research Institute
}
} Title-Subject: [Filtered] LR White Ultrathin Sections Prepared for ImmunoGold
}
} Message: Hello,
} Second Request - Not sure if the first message was transmitted...
} What is the maximum time I need to wait in order to apply immunogold reagents
} (buffers, etc.) to
} ultrathin sections of LR White blocks (Medium hardness with catalyst?
} Thanks much,
} Vickie
}

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From: les-at-zsgenetics.com
Date: Tue, 17 Jan 2017 13:27:06 -0600
Subject: [Microscopy] curtain systems

Contents Retrieved from Microscopy Listserver Archives
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Hello,
I wanted to know if anyone has had experience using curtain wall systems to
segment a space for microscopes without building hard walls. I want to make
a somewhat-temporary home for an instrument. Laser safety curtaining is
common but way too expensive and unnecessary, since we have no lasers. Has
anyone used any of the industrial-type curtain systems to divide up a space?
Thanks for any information.

Regards,
Larry Scipioni
ZS Genetics


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From: bicarbaj-at-mtholyoke.edu
Date: Tue, 17 Jan 2017 13:53:10 -0600
Subject: [Microscopy] EM: wall-mounted fume extractors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

I am considering purchasing a wall-mounted fume extractor for my
microwave tissue processor which I will use for EM and paraffin work
(to save space in my fume hood).

I have found some filters that have been rated for use with PFA and
GA, but I am waiting to hear from the manufacturer regarding their use
for OSO4, acetone, resins, and PO (doubt that info is available). I'd
like to assume that the filters ("special carbon-treated") effectively
filter out all of these, but I need to be as certain as possible. I
will have undergraduates preparing samples and hope to use the
microwave in my TEM and SEM courses, so I am very concerned about
student safety.

Do any of you have any experience using these systems? Are they safe?
Should I stop being so worried and just buy it? :)

Thank you!
-Blanca
-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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From: vray-at-partbeamsystech.com
Date: Tue, 17 Jan 2017 15:06:28 -0600
Subject: [Microscopy] Re: EM: wall-mounted fume extractors

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Blanca,

Why don't you run HVAC flex pipe from your wall-mounted fume extractor
and plug it into the exhaust system? Failing that - modify one frame in
the nearest window and exhaust filtered air outside. Decent carpenter or
HVAC tech should be able to make such setup. Be safe :)


Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 1/17/2017 2:54 PM, bicarbaj-at-mtholyoke.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello everyone,
}
} I am considering purchasing a wall-mounted fume extractor for my
} microwave tissue processor which I will use for EM and paraffin work
} (to save space in my fume hood).
}
} I have found some filters that have been rated for use with PFA and
} GA, but I am waiting to hear from the manufacturer regarding their use
} for OSO4, acetone, resins, and PO (doubt that info is available). I'd
} like to assume that the filters ("special carbon-treated") effectively
} filter out all of these, but I need to be as certain as possible. I
} will have undergraduates preparing samples and hope to use the
} microwave in my TEM and SEM courses, so I am very concerned about
} student safety.
}
} Do any of you have any experience using these systems? Are they safe?
} Should I stop being so worried and just buy it? :)
}
} Thank you!
} -Blanca
} -----------------------------------------
} Blanca Carbajal Gonzalez, M.S.
} Director of Microscopy
} Science Center
} 50 College St
} Mount Holyoke College
} Clapp Laboratory 123
} Office: 413-538-3118
} Cell: 559-905-7138
} bicarbaj-at-mtholyoke.edu
} MHC Microscopy
}
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} 5, 37 -- From: Blanca Carbajal Gonzalez {bicarbaj-at-mtholyoke.edu}
} 5, 37 -- Date: Tue, 17 Jan 2017 14:57:50 -0500
} 5, 37 -- Message-ID: {CAO94sumJEJEG0mw+2yoewmQSQRDodJE5CkOazdHvbhxzZB9vvg-at-mail.gmail.com}
} 5, 37 -- Subject: EM: wall-mounted fume extractors
} 5, 37 -- To: microscopy-at-microscopy.com
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5, 35 -- From vray-at-partbeamsystech.com Tue Jan 17 15:06:27 2017
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5, 35 -- Subject: Re: [Microscopy] EM: wall-mounted fume extractors
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5, 35 -- References: {201701171954.v0HJsT6C009676-at-microscopy.com}
5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com}
5, 35 -- Cc: microscopy-at-microscopy.com
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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 Jan 2017 18:08:32 -0600
Subject: [Microscopy] viaWWW CryoEM :Position available in San Diego, California

Contents Retrieved from Microscopy Listserver Archives
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X-from: mreedman-at-rms.org.uk

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Email: mreedman-at-rms.org.uk Name: Mel Reedman

Organization: Royal Microscopical Society

Title-Subject: [Filtered] MSM XX Abstract Submission Extended - Microscopy of Semi Conducting Materials

Message: Abstract deadline extended to Sunday 22 January and Registration now open!
Microscopy of Semiconducting Materials XX (MSM XX)
9-13 April 2017
Lady Margaret Hall, University of Oxford

www.rms.org.uk/msm-xx

Scientific Organiser: Thomas Walther
The conference will focus on the most recent advances in the study of the structural and electronic
properties of semiconducting materials by the application of transmission and scanning electron
microscopy. The latest developments in the use of other important micro-characterisation techniques
including scanning probe microscopy, X-ray topography and diffraction will also be featured.
Developments in materials science and technology covering the complete range of elemental and
compound semiconductors will be described.
Submit your abstract before midnight on Sunday 22 January 2017.
We are accepting abstracts for both submitted talks and poster presentations at MSM XX on the
following topics:
- Analytical TEM
- CL
- Lattice Defects
- Poly and Nano Crystals
- Thin Films
- Strained Layers and QWs
- Nanowires
- SPM & APFIM
- SEM & FIB
- Advanced Devices

Registration
Registration for this meeting is now open. To register your place, please visit www.rms.org.uk/msm-xx.
The registration fees are as follows:

Standard rate 575
RMS/IoP Member rate 475
Student rate 325
RMS/IoP Student member rate 280

To book en-suite accommodation for 75 a night directly with Lady Margaret Hall, please visit
http://conference.lmh.ox.ac.uk/accommodation/. Use the code MSM2017, to show availability and take
advantage of the preferential room rate.
Find out more, submit an abstract and register at www.rms.org.uk/msm-xx


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18, 53 -- Subject: viaWWW:MSM XX Abstract Submission Extended - Microscopy of Semi
18, 53 -- Conducting Materials
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From ronasyre474-at-gmail.com Wed Jan 18 09:59:01 2017
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Email: erica-at-scripps.edu Name: Erica Ollmann Saphire

Organization: The Scripps Research Institute

Title-Subject: [Filtered] Position available in San Diego, California

Message: Position available for an experienced staff scientist (or postdoc) to perform single
particle cryoEM studies on proteins relevant for viral hemorrhagic fever pathogenesis and medical
defense.

You will have primary responsibility for data collection and oversight of a dedicated 300 keV Titan
Halo with a Falcon 2 direct detector and for training of incoming students to the lab.
In particular, we are looking for someone with strong interest and expertise in instrumentation,
good communication skills, and a collaborative spirit who could pursue their own projects and assist
those of others.

Our lab studies the proteins of viruses like Ebola, Marburg and Lassa, using structural biology and
cellular analysis to understand how the very few proteins encoded by these viruses achieve a
tremendous array of functions through the virus life cycle. Although we are primarily a structural
biology lab ourselves, we direct a five-continent collaboration of academic, industrial and
government scientists and clinicians. Hence, there is the opportunity here to see how the structures
fit into the cellular, organismal and population contexts and to use structural biology as a roadmap
to devlop much-needed vaccines and therapeutics.

Applications should be sent via email to erica-at-scripps.edu and should include a c.v., list of
publications, names of references and a letter of interest. If you have any questions, just email me!

Erica Ollmann Saphire, Ph.D.
Professor, Immunology & Microbial Science
Co-Director, Center of Excellence, The Global Virus Network
Director, Viral Hemorrhagic Fever Immunotherapeutic Consortium

The Scripps Research Institute
10550 North Torrey Pines Road, IMM-21
La Jolla, CA 92037
Tel: (858)784-8602
erica-at-scripps.edu

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17, 52 -- Subject: viaWWW CryoEM :Position available in San Diego, California
17, 52 -- References: {201701192020.v0JKKk56030157-at-microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 Jan 2017 18:10:12 -0600
Subject: [Microscopy] viaWWW:TEM Application Scientist Opening

Contents Retrieved from Microscopy Listserver Archives
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X-from: jhyun-at-gatan.com

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] TEM Application Scientist Opening

Message: Location: Pleasanton, CA (remote locations considered)

Position Summary:
This position is responsible for supporting the sales and marketing efforts of Gatan’s highly
successful Analytical TEM product line (GIF and EELS systems). The position is based at Gatan’s
Pleasanton, CA headquarters but will support analytical products worldwide.

Job Responsibilities:
•Provide pre-sales applications support with presentations and demonstrations of Gatan products on a
range of TEM models
•Provide post-sales customer support and on-site customer training
•Provide on-site advanced customer training and telephone/email support
•Provide general applications support, telephone/email support, and limited hardware and software
troubleshooting
•Propose design enhancements and improvements to Gatan hardware and software
•Work with Gatan R&D in the development and testing of new products and applications
•Represent Gatan at scientific conferences

Education/Experience:
•Advanced degree in science or engineering (or equivalent experience) is required
•Strong background in transmission electron microscopy, specifically in relation to the acquisition,
application, interpretation, and discussion of analytical TEM techniques including EELS, EDS, EFTEM,
and STEM techniques
•Hands-on, post-graduate level experience in advanced analytical TEM applications with a proven
publication record Qualifications:
•Ability to interface effectively with customers at all experience levels while projecting a strong
client service attitude
•Excellent verbal, written, and interpersonal communication skills in English are essential
•Ability to work on complex learning and development problems as well as teach highly technical
information is essential
•Significant domestic and international travel required- 50% or higher at times.

To Apply:
Interested candidates should submit a resume to hrus-at-gatan.com.
Gatan is an equal opportunity employer. All qualified applicants will receive consideration for
employment without regard to race, religion, color, national origin, sex, sexual orientation, gender
identity, age, status as a protected veteran, among other things, or status as a qualified
individual with disability.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Jan 2017 14:51:53 -0600
Subject: [Microscopy] viaWWW: Gatan ChromaCL Help Needed

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Email: heckman-at-bgsu.edu
Name: Carol Heckman

Organization: Bowling Green State University

Title-Subject: [Filtered] Gatan ChromaCL

Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our
Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need
cathodoluminescence mode for several projects.

Does anybody have the same problem? If so, what did you do and did you solve it?


Carol Heckman


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From: james.passmore-at-sealedair.com
Date: Mon, 23 Jan 2017 08:10:43 -0600
Subject: [Microscopy] XPS/ESCA system for donation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Carol,

Since the problem is intermittent and occurs during insertion, chances
are you have either crack in vacuum bellows, or warn-out O-ring
somewhere in feedthrough. You would probably need to call in either OEM
or third-party service, or find someone local who can bring in a leak
detector and troubleshoot this on site.

Best Wishes :)

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 1/21/2017 3:53 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: heckman-at-bgsu.edu
} Name: Carol Heckman
}
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}
} Title-Subject: [Filtered] Gatan ChromaCL
}
} Message: We bought a GATAN cathodoluminescence detector some years ago, and had it installed on our
} Hitachi 2700S SEM. It breaks vacuum sometimes when it is entered into the column. We need
} cathodoluminescence mode for several projects.
}
} Does anybody have the same problem? If so, what did you do and did you solve it?
}
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} Carol Heckman
}
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5, 35 -- From vray-at-partbeamsystech.com Sat Jan 21 16:57:54 2017
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5, 35 -- To: heckman-at-bgsu.edu
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5, 35 -- Cc: microscopy-at-microscopy.com
5, 35 -- From: Valery Ray {vray-at-partbeamsystech.com}
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From sawijama5-at-gmail.com Sat Jan 21 19:21:49 2017
Return-Path: {sawijama5-at-gmail.com}
Received: from gmail.com ([210.92.18.86])
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0M1LkoN032594
for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 21 Jan 2017 19:21:48 -0600
Message-ID: {18CCB40A.A1DE92E8-at-gmail.com}

All,
For those interested in the microanalysis side of things, I have
available to donate a Physical Electronics Quantum 2000 Scanning ESCA
Microprobe. The Quantum is the predecessor to the PHI Quantera model.

This X-ray Photoelectron Spectroscopy/Electron Spectroscopy for
Chemical Analysis system has been sitting powered down but connected
to clean nitrogen for a little over a year, after developing a problem
in a small power supply. That power supply has been priced at about
$300. Prior to that, it had a noise issue, so I believe there is a
problem with the detector or in the detector electronics that would
need to be addressed. The unit was purchased in 1999 for
approximately $600,000, and used for several years with very clean
samples. In 2006 it was upgraded to a Windows XP data system.

My company is relocating R&D facilities from Duncan, SC (the current
instrument location) and no longer has pressing need for the
technique. Rather than relocate the instrument, we would like to
donate it to a university or other non-profit. Recipient would be
responsible for transportation and related costs. As part of an
effort to give back to our local community, preference would be given
to schools in closer proximity to our new headquarters in Charlotte,
NC, but all interested parties are encouraged to inquire by emailing
me at james.passmore+xps-at-sealedair.com.

Regards,
Jim

--
Jim Passmore
Principal Scientist, R&D

Sealed Air Corporation
P: 864.433.2927
100 Rogers Bridge Rd., Bldg A
Duncan, SC 29334

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From: microscopy.listserver-at-gmail.com
Date: Mon, 23 Jan 2017 18:14:02 -0600
Subject: [Microscopy] viaWWW: Position Open : Research Associate I (28N) - Electron

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Email: cni-at-udel.edu Name: Chaoying Ni

Organization: University of Delaware

Title-Subject: [Filtered] Research Associate I (28N) - Electron Microscopy

Message: Research Associate I - (Grade 28N) Materials Science and Engineering

Deadline: January 26, 2017

CONTEXT OF THE JOB:

Under limited supervision, performs a variety of complex technical laboratory duties including the
design and construction of test equipment. Supervises and trains personnel. The principal emphasis
is on working independently and exercising personal initiative in conducting experiments using
complex electron and light microscopes, scanning probe microscopes and associated sample preparation
equipment and experiment devices.

QUALIFICATIONS:

Requires a minimum of a Bachelor's degree and one year of experience in laboratory work in a
relevant field as well as experience with the necessary physical, electronic and chemical systems.
Supervisory experience preferred.
Related progressive experience beyond a high school diploma or GED may be substituted for required
education or additional related education may be substituted for required experience. Requires
knowledge of laboratory techniques, procedures and instrumentation.

Requires abilities to read and interpret complex operations manuals, drawings and design, interpret
problems and design equipment for the particular tasks, assume responsibility and take initiative to
perform tasks, work independently, train and instruct others, and communicate effectively and
interact well with people of all ages and diverse backgrounds.

OCCUPATIONAL EXPOSURES:

May be required to use personal protective equipment to prevent exposure to hazardous materials.


More details:
https://udjobs.nss.udel.edu:4450/psc/RESUME/EMPLOYEE/HRMS/s/WEBLIB_UDEL.ISCRIPT1.FieldFormula.IScript_Careers?Page=HRS_CE_HM_PRE&Action=A&SiteId=888&

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From: microscopy.listserver-at-gmail.com
Date: Mon, 23 Jan 2017 18:15:00 -0600
Subject: [Microscopy] viaWWW:Nano Technology Sales Position

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Email: mikeh-at-ncimicro.com Name: Mike Hehr

Organization: NCI

Title-Subject: [Filtered] Nano Technology Sales Position

Message: We are currently seeking an individual with EM Sample Prep experience for a new technical
sales position. This includes, Ion Milling, Ultra Microtomy, CLEM and High Pressure Freezing. This
role will be to support customers in the vast Midwest. If interested, please submit you resume to
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From: jcarroll-at-murraystate.edu
Date: Wed, 25 Jan 2017 14:29:42 -0600
Subject: [Microscopy] TEM negative staining issues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Those of you actively working with FIB in either device or analytical applications could find the forwarded below message of interest:

--- On Tue, 1/24/17, Hugo Bender (imec) {Hugo.Bender-at-imec.be} wrote:

} From: Hugo Bender (imec) {Hugo.Bender-at-imec.be}
} Subject: new link to EFUG website : http://efug.imec.be/
} To: "Hugo Bender (imec)" {Hugo.Bender-at-imec.be}
} Date: Tuesday, January 24, 2017, 1:18 AM
}
} Dear EFUG’ers,
} FIB’ers,
}  
}  
} Please note that the link to the
} EFUG website is changed to : http://efug.imec.be/
}
} The old link will soon not be
} operational anymore.  So update your bookmarks
} now!
}  
}  
} There will be again 2 FIB meetings
} in Europe in 2017:
}  
} 1st  EUFN
} workshop
} (Former DACH
} Workshop) : general FIB and FIB
} applications
} 4-5 July 2017, Graz, Austria.
}
} Abstract : deadline 30 April 2017
} http://www.eu-f-n.org/
}
}
} 21st
} EFUG meeting
} during ESREF        :
} semiconductor and device applications of
} FIB
} Week 25-29 September 2017,
} Bordeaux France
} http://efug.imec.be/
}  
}  
} Please forward to your FIB
} colleagues !
}  
}  
} Best regards
}  
} Hugo
}  
}  
} Hugo
} Bender
} Principal Member
} Technical Staff
} T +32 16 28
} 1304
} hugo.bender-at-imec.be 
} I 
} www.imec.be
} Kapeldreef 75 
}
} I  3001
} Leuven 
} I 
} Belgium
}
} imec e-mail
} disclaimer
}  
}
}  
}
}
}


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From michcons458-at-gmail.com Tue Jan 24 16:35:33 2017
Return-Path: {michcons458-at-gmail.com}
Received: from gmail.com ([210.92.18.86])
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v0OMZUvo027260
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Message-ID: {4149520D.C242A3C7-at-gmail.com}

I am a relatively inexperienced TEM tech and am having problems with my
PTA. Each time I make fresh PTA, I end up with an image full of stain
blobs. Obviously this is useless. I have tried purchasing fresh PTA, but
that didn't make any difference. I have also used BSA, which helps some,
but not a lot. Help! Any ideas are welcome.

Jami

Jami Carroll
Lab Tech II - Virology
Breathitt Veterinary Center
Hopkinsville, KY
270-886-3959
jcarroll-at-murraystate.edu

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From: FMonson-at-wcupa.edu
Date: Wed, 25 Jan 2017 15:42:07 -0600
Subject: [Microscopy] TEM negative staining issues

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Jami,

I do hope that you have consulted Google with: "PTA negative staining" to try to find some troubleshooting advice from experts. What I am about to offer is all true and necessary for me. Thus, necessary for everyone else - if I don't think about your problem.

I can state with absolutely strictly personal experience, that one - Me! - throws time away all the time if the water one - Me, again - starts with has not been deionized, glass distilled, filtered with the 22um ['micro' or 'Meuw' meter] 57mm Millipore filter, and, if to be used in MolBio, laced with RNAse Inhibitor [https://en.wikipedia.org/wiki/Ribonuclease_inhibitor ]. Once this 'Monkish' procedure has been completed with both song and dance, I can be assured of a good experiment.

If the 'blobs' are larger than .22um, then I recommend that you run your PTA thru a 0.22um 12mm Millipore filter using a small Swinney holder with a 1ml disposable syringe. You could also try a dose of high frequency sonic jewelry cleaner borrowed from your Mom or your 'gal.'

I have given you both mystic and sound recommendations to attack your problem. These are things I have tried in the past, but, I admit, nowhere near Kentucky. [In the old days, histologists who Wintered in Maine and Summered in Florida, had two types of paraffin: one for Maine; and a second for Florida - each with a 'Climatized' paraffin recipe!

I do humarize when the subject permits, so, please understand that I am old (77), sometime called "Grumpy39," and cannot restrain myself any more.

Cheers, and good luck,

Fred Monson

P.S. If you come to a moment where you simply have to change brains, sit down and answer this simple question: "How many digital cameras do you have with you right now? After you answer, please take a few minutes to determine why some old guy would ask such a silly question to take my mind of my problem with PTA. There you go. My answer is that 98% of educated people give the wrong answer. You should know that I only gave ONE multiple answer test in the 12 years that I instructed in biology in development and electron microscopy. In histology, I felt obligated to remind students that all they would learn in histology would be about dead stuff. Cheers, again.

P.S. With that cheerful business done, please remember that life/death of virions is still under misunderstanding. Be careful - always.
P.S. In the creek, doped with E. Coli, for each of them there are 100 phage.
P.S. Oh! Yes! If you have a need of concentrating the sample, consider a Beckman Airfuge with a TEM head.

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)
Fc.monson-at-gmail.com for more help if you need it: i.e., refereces, and other stuff.






-----Original Message-----
X-from: jcarroll-at-murraystate.edu [mailto:jcarroll-at-murraystate.edu]
Sent: Wednesday, January 25, 2017 3:41 PM
To: Monson, Frederick {FMonson-at-wcupa.edu}

I am a relatively inexperienced TEM tech and am having problems with my PTA. Each time I make fresh PTA, I end up with an image full of stain blobs. Obviously this is useless. I have tried purchasing fresh PTA, but that didn't make any difference. I have also used BSA, which helps some, but not a lot. Help! Any ideas are welcome.

Jami

Jami Carroll
Lab Tech II - Virology
Breathitt Veterinary Center
Hopkinsville, KY
270-886-3959
jcarroll-at-murraystate.edu

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From: rongchigram79-at-yahoo.com.sg
Date: Thu, 26 Jan 2017 02:46:34 -0600
Subject: [Microscopy] Temperature Logger to Recommend

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Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.

Cheers,
Yee Yan, Tay
FACTS Lab
NTU
Singapore

==============================Original Headers==============================
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From: leunissen-at-aurion.nl
Date: Thu, 26 Jan 2017 03:04:45 -0600
Subject: [Microscopy] Re: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

you may want to talk to Guenter Resch of Nexperion. He might be able to help.
His email address: guenter.resch-at-nexperion.net

Cheers,

Jan Leunissen

} On 26/01/2017, at 21:46, rongchigram79-at-yahoo.com.sg wrote:
}
}
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} Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
}
} Cheers,
} Yee Yan, Tay
} FACTS Lab
} NTU
} Singapore
}
} ==============================Original Headers==============================
} 2, 29 -- From rongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017
} 2, 29 -- Received: from nm11-vm7.bullet.mail.sg3.yahoo.com (nm11-vm7.bullet.mail.sg3.yahoo.com [106.10.148.246])
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From: vray-at-partbeamsystech.com
Date: Thu, 26 Jan 2017 10:45:11 -0600
Subject: [Microscopy] Fwd: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have used Extech RH520A temperature/humidity data logger in FIB room
and was very pleased with it in all respects. Initial setup required
some programming, but features and display/download capabilities were
excellent:

http://www.extech.com/display/?id=14702

Valery Ray
www.linkedin.com/in/valeryray {http://www.linkedin.com/in/valeryray}
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message
Mobie: +1-978-305-0479 {tel:%2B1-978-305-0479} - leave a message
E-mail: vray-at-partbeamsystech.com {mailto:vray-at-partbeamsystech.com}
Web: www.partbeamsystech.com {http://www.partbeamsystech.com}
Web: www.freudlabs.com {http://www.freudlabs.com}


On 1/26/2017 4:06 AM, leunissen-at-aurion.nl wrote:
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} Hello,
}
} you may want to talk to Guenter Resch of Nexperion. He might be able to help.
} His email address:guenter.resch-at-nexperion.net
}
} Cheers,
}
} Jan Leunissen
}
} } On 26/01/2017, at 21:46,rongchigram79-at-yahoo.com.sg wrote:
} }
} }
} }
} }
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} }
} } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
} }
} } Cheers,
} } Yee Yan, Tay
} } FACTS Lab
} } NTU
} } Singapore
} }
} } ==============================Original Headers==============================
} } 2, 29 -- Fromrongchigram79-at-yahoo.com.sg Thu Jan 26 02:46:34 2017
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} } 2, 29 -- From: YY YY {rongchigram79-at-yahoo.com.sg}
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} 7, 35 -- Fromleunissen-at-aurion.nl Thu Jan 26 03:04:45 2017
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From: John.Mardinly-at-asu.edu
Date: Fri, 27 Jan 2017 10:30:47 -0600
Subject: [Microscopy] Re: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello
Anybody knows what are the sizes of the 3 holes of the strip aperture of the
Jeol 5600LV?
Thanks for your time!
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************







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8, 20 -- From eikonika-at-otenet.gr Fri Jan 27 03:23:23 2017
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From annepenn4-at-gmail.com Fri Jan 27 06:05:02 2017
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} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun!
}
}
}
} John Mardinly, Ph.D., P.E., Retired ASU
}
}
}
} } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote:
} }
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} }
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} } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
} }
} } Cheers,
} } Yee Yan, Tay
} } FACTS Lab
} } NTU
} } Singapore
} }
} } ==============================Original Headers==============================
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From: WHITTAKS-at-si.edu
Date: Fri, 27 Jan 2017 10:42:14 -0600
Subject: [Microscopy] Re: Temperature Logger to Recommend

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


I have several units from ITWatchdogs. Never had any problem and they email and text me and the building managers when there is a thermal issue out of range. Interface is a standard web browser.

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY



-----Original Message-----
X-from: John.Mardinly-at-asu.edu [mailto:John.Mardinly-at-asu.edu]
Sent: Friday, January 27, 2017 11:38 AM
To: Whittaker, Scott {WHITTAKS-at-si.edu}


} I recommend a temperature sensor from Vernier.com. Their line of Go Direct sensors connect to a laptop by USB, are dirt cheap ($39 for the one I have), and come with a very nice free software package (I have never seen it crash!) for recording your measurements. I see now they have options for wireless with iOS devices. Have fun!
}
}
}
} John Mardinly, Ph.D., P.E., Retired ASU
}
}
}
} } On Jan 26, 2017, at 1:59 AM, rongchigram79-at-yahoo.com.sg wrote:
} }
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} }
} } Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.
} }
} } Cheers,
} } Yee Yan, Tay
} } FACTS Lab
} } NTU
} } Singapore
} }
} } ==============================Original
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From: microscopy.listserver-at-gmail.com
Date: Tue, 31 Jan 2017 07:37:58 -0600
Subject: [Microscopy] viaWWW:HA tag - live imaging

Contents Retrieved from Microscopy Listserver Archives
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Email: stirling.newberry-at-gmail.com Name: Stirling Newberry

Organization: MIT

Title-Subject: [Filtered] Sterling P Newberry

Message: Colleagues, we regret to note the passing of
Sterling Newberry a long time member of the microscopy
community whose passion for microscopy and education
will be missed.


https://en.wikipedia.org/wiki/Sterling_Newberry

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To: microscopylistserverarchive6-at-microscopy.com

Thanks to all who responded. We have found a new home for this
system. If arrangements fall through, we will select another choice
from the previous inquiries.

Regards,
Jim

--
Jim Passmore
Principal Scientist, R&D

Sealed Air Corporation
P: 864.433.2927
100 Rogers Bridge Rd., Bldg A
Duncan, SC 29334

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From smithdiana157-at-gmail.com Mon Jan 30 11:59:01 2017
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X-from: leandro.lemgrubersoares-at-glasgow.ac.uk

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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: University of Glasgow

Title-Subject: [Filtered] HA tag - live imaging

Message: Dear All,
does anyone know if there is a membrane permeable dye or probe that recognises HA-tag protein in
live imaging?

Thanks!

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From: henning.stahlberg-at-unibas.ch
Date: Tue, 31 Jan 2017 14:38:41 -0600
Subject: [Microscopy] Fwd: [3dem] GRC Locations

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


} Begin forwarded message:
}
} From: Nancy Gray {ngray-at-grc.org}
} Subject: Re: [3dem] GRC Locations
} Date: January 31, 2017 at 19:19:24 GMT+1
} To: "Smith, Phillip R." {smithp01-at-nyumc.org} , Marin van Heel {marin.vanheel-at-googlemail.com}
} Cc: Henning Stahlberg {henning.stahlberg-at-unibas.ch} , Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu} , "CCPEM-at-JISCMAIL.AC.UK" {CCPEM-at-JISCMAIL.AC.UK}
}
} Folks,
} Could you kindly forward my response to your network since my attempt was rejected since I am not authorized. I am getting many emails on the issue and would like all to know GRC is formulating a response. Many thanks,
} Nancy
}
} Nancy Ryan Gray, PhD
} Gordon Research Conferences, President and Chief Executive Officer
} 512 Liberty Lane
} West Kingston, RI 02892
} 401-360-1521
} www.grc.org
}
}
} } On Jan 31, 2017, at 11:33 AM, Nancy Gray {ngray-at-grc.org} wrote:
} }
} } Dear Henning, Ulrike, Stefan, Marin, Ross and members of the 3dem mailing list,
} }
} } Thank you for your input, it is indeed a difficult situation. I have scheduled a conference call with the GRC Board of Trustees Executive Committee and GRC Legal Counsel to address these concerns and will get back to you shortly.
} } Thanks for your support, patience and understanding.
} } Nancy
} }
} }
} }
} } Nancy Ryan Gray, PhD, President and Chief Executive Officer
} } Gordon Research Conferences
} } 512 Liberty Lane, West Kingston, RI 02892-1502
} } Phone: 401-360-1521 l Fax: 401-783-7644
} } Email: ngray-at-grc.org l Web: http://www.grc.org

[]


} } } From: Henning Stahlberg {henning.stahlberg-at-unibas.ch}
} } } Subject: [3dem] GRC Locations
} } } Date: January 30, 2017 at 21:08:10 GMT+1
} } } To: "Nancy R. Gray" {NGray-at-grc.org}
} } } Cc: Microscopy {Microscopy-at-microscopy.com} , 3dem {3dem-at-ncmir.ucsd.edu}
} } }
} } } Dear Nancy,
} } }
} } } Science is open and international. The US is currently not, unfortunately.
} } } Until that situation is resolved, may I ask you to consider moving the upcoming GRC conferences to openly accessible locations, such as international conference sites as in Cancun in Mexico or in other countries.
} } } This would allow fair and equal access of scientist from all countries and religions to your conferences, and prevent a boycott movement from forming.
} } } Your conferences in Europe and Asia are a great start.
} } }
} } } With best wishes,
} } }
} } } Henning Stahlberg.
} } }
} } }
} } } Henning Stahlberg, PhD
} } } Prof. for Structural Biology, C-CINA, Biozentrum, University Basel
} } } Mattenstrasse 26 | D-BSSE | WRO-1058 | CH-4058 Basel | Switzerland
} } } http://c-cina.org | Tel. +41-61-387 32 62
} } }


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From: microscopy.listserver-at-gmail.com
Date: Tue, 31 Jan 2017 16:58:43 -0600
Subject: [Microscopy] viaWWW:Histo / EM position open

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X-from: erosen-at-mednet.ucla.edu

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Email: erosen-at-mednet.ucla.edu Name: Eric Rosen
Organization: UCLA Medical Center
Title-Subject: [Filtered] Histo / EM position open
Message: Hi all,

We have a 50% Histo/50% EM position open at UCLA hospital.

See job description here:
http://www.uclahealthcareers.org/all-jobs/Histotechnologist-II/H88333
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From: jacques.faerber-at-ipcms.unistra.fr
Date: Wed, 1 Feb 2017 03:19:35 -0600
Subject: [Microscopy] EDAX .spc to .emsa format batch conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all

One day too late, but anyway happy new year to all, as it is my first
post of the year !

I'm looking if someone could have soon written a bash script (or else
working on linux system) to convert in batch mode EDAX spectra from the
.spc file format to .emsa/msa.
The "Export" function of the TEAM software has only the .spc format as
output, and the "Send_To_Folder" function is far too time consuming for
saving a project part with tenth of spectra, in particular as it doesn't
keep the original names of the data.

I tried Spectrum Viewer but I didn't bring it to work on linux (via
Wine, but I'm very binary with Wine : it works or doesn't work... I've
no time to learn to configure it !).

Fortunately, DTSA-II reads the .spc format and can save in emsa, what
allows me to work. But DTSA has not the goal to do batch format
conversions !

Thanks to all

Jacques

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


==============================Original Headers==============================
10, 26 -- From jacques.faerber-at-ipcms.unistra.fr Wed Feb 1 03:19:35 2017
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From: joshua.taillon-at-nist.gov
Date: Wed, 1 Feb 2017 08:39:38 -0600
Subject: [Microscopy] EDAX .spc to .emsa format batch conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jacques,

The open-source HyperSpy software (www.hyperspy.org) can do what you are looking for.
EDAX file reading (both .spd and .spc) has been added in the latest development builds (and should be included in the next minor release -- v1.2). If you cannot wait for that, setting up a copy of the development version isn't too difficult if you have some proficiency with Python and git (http://hyperspy.org/hyperspy-doc/current/user_guide/install.html#development-version).

Good luck!

- Josh

NB: Any mention of commercial products or software is for information only; it does not imply recommendation or endorsement by NIST.

--
Joshua Taillon, Ph.D.
Materials Measurement Science Division
National Institute of Standards and Technology

100 Bureau Drive, MS-8372
Gaithersburg, MD 20899
W: (301) 975-2913
E: joshua.taillon-at-nist.gov



-----Original Message-----
X-from: jacques.faerber-at-ipcms.unistra.fr [mailto:jacques.faerber-at-ipcms.unistra.fr]
Sent: Wednesday, February 1, 2017 4:42 AM
To: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}

Hi all

One day too late, but anyway happy new year to all, as it is my first post of the year !

I'm looking if someone could have soon written a bash script (or else working on linux system) to convert in batch mode EDAX spectra from the .spc file format to .emsa/msa.
The "Export" function of the TEAM software has only the .spc format as output, and the "Send_To_Folder" function is far too time consuming for saving a project part with tenth of spectra, in particular as it doesn't keep the original names of the data.

I tried Spectrum Viewer but I didn't bring it to work on linux (via Wine, but I'm very binary with Wine : it works or doesn't work... I've no time to learn to configure it !).

Fortunately, DTSA-II reads the .spc format and can save in emsa, what allows me to work. But DTSA has not the goal to do batch format conversions !

Thanks to all

Jacques

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


==============================Original Headers==============================
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==============================Original Headers==============================
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26, 56 -- From: "Taillon, Joshua A. (Fed)" {joshua.taillon-at-nist.gov}
26, 56 -- To: "jacques.faerber-at-ipcms.unistra.fr" {jacques.faerber-at-ipcms.unistra.fr}
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26, 56 -- Subject: RE: [Microscopy] EDAX .spc to .emsa format batch conversion
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From: vray-at-partbeamsystech.com
Date: Wed, 1 Feb 2017 10:28:12 -0600
Subject: [Microscopy] Re: EDAX .spc to .emsa format batch conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jacques,

Unless someone have already written and would share the script you need
for converting .spc to .emsa, it should be possible to automate
sequential opening of files within a folder by DTSA-II, saving data in
another format, and repeating the operation until all files are
processed with one of GUI scripting tools. I am using WinBatch (US$100
from http://www.windowware.com/, no connection just a happy user) for
file processing purposes, although in different then yours application.
There is also AutoIt which is free from www.autoitscript.com, but I did
not try it. Not aware of similar GUI scripting tools for Linux, but
would be surprised if they didn't exist as well.

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
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Web: www.freudlabs.com

On 2/1/2017 4:20 AM, jacques.faerber-at-ipcms.unistra.fr wrote:
} ----------------------------------------------------------------------------
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} ----------------------------------------------------------------------------
}
} Hi all
}
} One day too late, but anyway happy new year to all, as it is my first
} post of the year !
}
} I'm looking if someone could have soon written a bash script (or else
} working on linux system) to convert in batch mode EDAX spectra from the
} .spc file format to .emsa/msa.
} The "Export" function of the TEAM software has only the .spc format as
} output, and the "Send_To_Folder" function is far too time consuming for
} saving a project part with tenth of spectra, in particular as it doesn't
} keep the original names of the data.
}
} I tried Spectrum Viewer but I didn't bring it to work on linux (via
} Wine, but I'm very binary with Wine : it works or doesn't work... I've
} no time to learn to configure it !).
}
} Fortunately, DTSA-II reads the .spc format and can save in emsa, what
} allows me to work. But DTSA has not the goal to do batch format
} conversions !
}
} Thanks to all
}
} Jacques
}
} J. Faerber
} IPCMS-DSI
} Institut de Physique et Chimie des Matériaux de Strasbourg
} Département Surfaces et Interfaces
} 23, rue de Loess ; BP43
} 67034 Strasbourg CEDEX 2
} France
}
} Tel 00 33(0)3 88 10 71 01
} Fax 00 33(0)3 88 10 72 48
} E-mail : Jacques.Faerber-at-ipcms.unistra.fr
}
}
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4, 37 -- From: Valery Ray {vray-at-partbeamsystech.com}
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From: jrminter-at-gmail.com
Date: Wed, 1 Feb 2017 12:00:07 -0600
Subject: [Microscopy] 1 of 2 Print all In new window RE: EDAX .spc to msa spectrum conversion

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Jacques Faerber asked about a script for the conversion of EDAX .spc to msa
format.

DTSA-II has a nice scripting language (jython) that permits this. One can
also apply a DTSA calibrated detector instance to the spectrum. I sent Jacques
amd earlier version of this this morning. I want to make certain that the
formatting stays correct, so I created a public gist on GitHub. You can
obtain the script at:

https://gist.github.com/jrminter/a697f83bec3f37dfb824fb7126542c41


Hope this helps.

John Minter

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From: frank_karl-at-ardl.com
Date: Wed, 1 Feb 2017 14:04:53 -0600
Subject: [Microscopy] Our TEM needs a cool breeze.....

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,
Our 1 meg Gatan Camera (model 780 Duel vision) on our TEM is dying and we want to give it a transplant. I'm looking for a cooling fan.

It's the 780.PL6FA model, if that helps. It's from the turn of the century and if you have a one that's broken and the fan works or you have a spare fan or know of a replacement fan, I sure would like to hear from you.

Thanks in advance........
Frank Karl
ARDL
330-794-6600

This email and any of its attachments may contain confidential information intended only for the use of the addressee(s). If the reader of this email is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination or copying of this email is strictly prohibited. If you have received this email in error, please notify us by return email at info-at-ardl.com, permanently delete the email, and destroy any printouts. If this email contains test data and/or draft reports, you are hereby notified that only a signed original test report will contain official results, a copy of which resides in the project folder located at ARDL, Inc. Thank you. Akron Rubber Development Laboratory, Inc.



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From: mikepidg-at-gmail.com
Date: Wed, 1 Feb 2017 23:11:28 -0600
Subject: [Microscopy] job search

Contents Retrieved from Microscopy Listserver Archives
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I am an accomplished Electron Microscopy Technician, supervisor, and
research specialist with 25 years experience working in both clinical
and research environments looking for a position where my acquired
skills can be used for professional advancement.

Thank you,

Michael Pidgeon

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Feb 2017 23:22:41 -0600
Subject: [Microscopy] Administrivia: Please follow the ML Rules

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues:

Can I remind everyone about the Microscopy Listserver Rules which can be found at:

http://www.microscopy.com/MicroscopyListserver/FAQ.html

Posting of "looking for a position" emails has never been permitted. Please
do not do this.

There are job boards, one specifically hosted and run by the Microscopy Society
of America http://jobs.microscopy.org/ that fulfill this role.


Nestor
Your Friendly Neighborhood SysCop


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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Feb 2017 06:53:17 -0600
Subject: [Microscopy] viaWWW:Jeol strip aperture sizes

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X-from: nlamine6-at-hotmail.com

This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://microscopy.com/MLFormMail.html
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Email: nlamine6-at-hotmail.com Name: naitbouda

Organization: cdta

Title-Subject: [Filtered] strip

Message: Hello

hello the size of jeol 5600LV and JEOL 6360LV
IS THE SAME the Jeol strip apertures 3 holes are/
20micron -30um-100um

you can use the size of
Jeol strip aperture 9.0x2.35x0.10mm (platinium-Iridium)

Part:67001-10-20-100


best regards
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Listserver Email Form V - 20120416
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From: rjpalmer-at-dir.nidcr.nih.gov
Date: Thu, 2 Feb 2017 07:48:41 -0600
Subject: [Microscopy] FW: Fwd: [3dem] GRC Locations

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Dear Ms. Ryan -

Thank you for your efforts to disseminate your communications to a larger
audience. This is an important issue from many perspectives and I imagine
most subscribers to this list will be very interested in how the GRC
responds. I would suggest that scientific societies all ought to have a
measured and well thought out position on the current political situation,
and that those positions be made abundantly clear world-wide. From my
personal perspective, I feel boycotts are unhelpful in resolving larger
issues, especially when the boycotting group has little to no true
leverage other than to shoot itself in the foot.

Robert J. Palmer Jr., PhD
National Institute of Dental and Craniofacial Research
National Institutes of Health
Building 30, Room 331
Bethesda MD 20892
301-594-0025




On 1/31/17, 4:00 PM, "henning.stahlberg-at-unibas.ch"
{henning.stahlberg-at-unibas.ch} wrote:

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Feb 2017 11:24:15 -0600
Subject: [Microscopy] viaWWW:Leica Sales Opportunity - Southeast USA

Contents Retrieved from Microscopy Listserver Archives
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Email: lon.nelson-at-leica-microsystems.com Name: Lon Nelson

Organization: Leica Microsystems

Title-Subject: [Filtered] Leica Sales Agent / Dealer Opportunity - Southeast USA

Message: Leica Microsystems is currently seeking a commissioned Sales Agent or Dealer in the
Southeast USA for our Electron Microscopy Sample Preparation solutions.

https://tinyurl.com/leicaEMprep

Interested parties should contact Lon Nelson, Director of Sales, at lon.nelson-at-leica-microsystems.com.

Best regards,

Lon Nelson
Director of Sales – Microscopy
lon.nelson-at-leica-microsystems.com
M +1 224-628-2467 | F +1 847-607-3160
Leica Microsystems, Inc.
1700 Leider Ln | Buffalo Grove, IL 60089 (USA)


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From: ALawrence-at-i2at.msstate.edu
Date: Thu, 2 Feb 2017 11:28:23 -0600
Subject: [Microscopy] M&M 2017 meeting - Student Bursaries

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The deadline for paper submission for M&M 2017 is less than two weeks away and as you are making plans to attend the St.
Louis meetings (Aug. 6-10), please don't forget about MSA's student bursary program. Its purpose is to encourage students to
attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the
established microscopy community.

The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events. The jobs involve
such things as providing support in the different symposia, staffing the volunteer office; newsletter distribution, and helping
with vendor tutorial sign-up.

Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and
activities where they would like to help. There is an added bonus of $10 cash for each morning and/or afternoon session worked
to assist with meals and a meeting shirt.

If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" in section
2 of the registration form. Applicants for the bursaries must be members of MSA or MAS, enrolled as students at a recognized
educational institution, and pay their registration fee. Bursary space is limited, so sign-up early.

If anyone has any questions about the bursary program, or would like to participate, please contact Amanda
(alawrence-at-i2at.msstate.edu).

Also don't forget about the opportunities for meeting awards!


Amanda Lawrence
Institute for Imaging and Analytical Technologies
Mississippi State University
662-325-7998
alawrence-at-i2at.msstate.edu


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9, 32 -- Subject: M&M 2017 meeting - Student Bursaries
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From: zaluzec-at-microscopy.com
Date: Thu, 2 Feb 2017 16:09:59 -0600
Subject: [Microscopy] viaWWW:M&M2017 - Symposium A09 Standards, Reference Materials, and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: owen.neill-at-wsu.edu
To: Zaluzec-at-microscopy.com

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Email: owen.neill-at-wsu.edu Name: Owen Neill

Organization: Peter Hooper GeoAnalytical Lab, Washington State University

Title-Subject: [Filtered] M&M2017 - Symposium A09

Message: Dear colleagues,
Apologies for the cross-postings, but I hope 2017 is off to a good
start for everyone. Paper submission for the Microscopy and
Microanalysis 2017 Annual Meeting (St. Louis, MO, 6-10 August, 2017) is
now open, and will close on 15 February. My co-conveners and I would
like to draw your attention to:
Symposium A09, Standards, Reference Materials, and Their Applications
in Quantitative Microanalysis.
We are looking for submissions dealing with the synthesis, evaluation,
and need for new reference materials; evaluation, distribution, and
maintenance of existing reference materials; the use of standards in
quantitative microanalysis; and the application of quantitative
microanalytical techniques to solving analytical problems. A full
description of this session is included below. Papers may be submitted
via the M&M2017 website: http://www.microscopy.org/mandm/2017/
We are also pleased to announce the invited speakers for this symposium:
• John Hanchar, Department of Earth Sciences, Memorial University,
Newfoundland
• William Nachlas, Department of Earth Sciences, Syracuse University
• Timothy Rose, Department of Mineral Sciences, Smithsonian Institution
• Stephen Wilson, Reference Materials Program, United States Geological
Survey
We are very excited to hear from these experts in microanalytical
standards and quantitative microanalysis, and we look forward to hearing
about your work as well. See you in St. Louis!
The organizers of Symposium A09:
Julien Allaz, University of Colorado – Boulder
Anette von der Handt, University of Minnesota – Twin Cities
Owen Neill, Washington State University
Symposium Description:
Standards and reference materials are essential for obtaining accurate
quantitative compositional data from X-ray microanalysis by EPMA or SEM
(WDS/EDS), as well as from other microanalytical techniques (LA-ICP-MS,
SIMS, µ-XRF, FTIR, Raman spectroscopy, etc.). These materials must be
rigorously evaluated for their reference compositions and homogeneity,
must be widely available to the analytical community, and must be
properly maintained to avoid contamination or deterioration. We welcome
contributions on the synthesis, evaluation, distribution, and
maintenance of standards and reference materials, as well as their
appropriate use in microanalysis. We further encourage submissions on
standard-based applications of quantitative microanalysis, or on the
development of new quantitative microanalytical protocols.

Topics of interest include:
• The use of standards and reference materials in quantitative
microanalysis, and the needs of the analytical community for improving
such materials.
• Synthesis, evaluation, distribution, and maintenance of standards and
reference materials.
• Development of new protocols for microanalytical techniques. •
Applications of standard-based techniques to solving microanalytical
problems.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Feb 2017 22:38:57 -0600
Subject: [Microscopy] viaWWW: Need Gatan 794 Camera Controller

Contents Retrieved from Microscopy Listserver Archives
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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] dvanced EELS and EFTEM Training School -
France 2017

Message: The Advanced EELS and EFTEM Training School hosted by EDF R&D
is an intensive 4-day training school that incorporates lectures,
computer laboratories, and microscope practical classes to provide
participants with a comprehensive, hands-on training on advanced
electron energy loss spectroscopy (EELS) and energy-filtered
transmission electron microscopy (EFTEM) topics.

The practical sessions on the microscope will be given at the Materials
Ageing Institute. This course focuses on the advanced practice of EELS
based imaging and analysis in the (S)TEM microscope on advanced based
EELS techniques.

A good experience with electron microscopy and EELS is highly
recommended. By the end of the course, participants can expect to get
the most out of their EELS spectrometer system and know how to best
optimize the experimental conditions in order to capture the maximum
amount of information out of the TEM samples using EELS.

Online information and registration:
http://www.gatan.com/company/events/advanced-eels-and-eftem-training-school-france-2017


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Email: jwsandinod-at-unal.edu.co Name: John W. Sandino

Organization: Universidad Nacional de Colombia

Title-Subject: [Filtered] Need Gatan 794 Camera Control

Message: Two years ago we received an old 794 retractable camera Gatan
as donation from the Tribenberg Lab (closed).
But two days ago the camera controller of this camera does not work any
more. Today the gatan people confirm the news and told as that the
solution would buy a new camera. However we don't have budget for that.

We ask for some who could have a camara contoller that coud give us for
free.
I know that it is no the best petition but for the moment is the unique
solution to work with our tecnai 20. one of the best microscopes in our
country Colombia.

Thanks
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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Feb 2017 22:43:21 -0600
Subject: [Microscopy] viaWWW:mmc2017 and EMAG 2017 Abstract Submission Deadline - 17

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Email: mreedman-at-rms.org.uk Name: Mel Reedman

Organization: Royal Microscopical Society

Title-Subject: [Filtered] mmc2017 Abstract Deadline Approaching

Message: mmc2017 and EMAG 2017 Abstract Submission Deadline - 17 February
---------------------------------------------------

The Microscience Microscopy Congress is returning to Manchester from 3 -
6 July 2017 and now is the time to join us at this great event by
submitting an abstract!

www.mmc-series.org.uk/abstracts
There is only 2 weeks left to submit an oral abstract as submission
closes on Friday 17 February 2017.

The Call for Papers covers a wide variety of topics and techniques as
you can see below:

Life Sciences Sessions
- Bio Applications: Investigating Biological Structure using Electron
Tomography
- Imaging in Flow Cytometry
- Imaging protein dynamics in living cells
- Investigating Biological Structure using Electron Tomography
- Bio Applications: Imaging Cells in 3D - matrix, tissue, in vivo
- Bio EM Sample Preparation and Analysis
- Bio Applications: Imaging Cancer
- Correlative Microscopy
- Bio-Applications: Long-term imaging using Single Plane Illumination
Microscopy
- Biological Processes at the Nanoscale (Celtic Session organised by MSI
and SMG)
- Microbial Imaging
- Host-Pathogen Interactions
- Bio Applications: Bacterial Ultrastructure
- Electron Cryomicroscopy of Biological Macromolecules
- Biological Applications of 3D Electron Microscopy
Frontiers in Bioimaging Sessions
- Bespoke light microscopy for molecular level imaging
- Advances in labelling for super-resolution microscopy
- Advanced light imaging for addressing longer length scale biological
questions
- Developing super-resolution methods for functional insight

Physical Sciences Sessions
- Earth, Environmental and Planetary Materials
- Microscopy of Engineered Surfaces and Tribology
- Energy and Energy Storage Materials
- Inorganic Nanomaterials (Celtic Session organised by MSI and SMG)

EMAG 2017 Keywords
Instrumentation
- 3-D microscopy
- in-situ microscopy techniques
- Low Dose Imaging and Analytical Microscopy
- Dynamic TEM
- Advances in SEM & FIB (CL, EBSD, beam deceleration, monochromators etc)
- Detector technologies and new instrumentation
- Vortex Beams and Phase plates
- Electron crystallography and diffraction
Materials
- Biological materials, Biomaterials and Soft matter
- Geological microscopy
- Catalytic materials
- Nanomaterials and 2D materials
- Energy and Energy Storage Materials
- Functional Materials
- Structural Materials and Metallurgy
- Surface imaging and modification
- Microscopy of interfaces and heterostructures
- High spatial resolution chemical and structural analysis

Scanning Probe Microscopy Sessions
- Frontiers of Scanning Probe Microscopy
- High Resolution SPM
- Structures, Interfaces and Mechanics in Life and Health with AFM
- SPM nano-mapping of materials properties

Not sure where your work would fit? You can view further details about
each conference session at
www.mmc-series.org.uk/conference/conference-sessions
Submit your abstract at www.mmc-series.org.uk/abstracts
Conference Registration is now open, book your place at
www.mmc-series.org.uk/registration

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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Feb 2017 22:44:09 -0600
Subject: [Microscopy] viaWWW:Oxford INCA EDS detector dead time 100%

Contents Retrieved from Microscopy Listserver Archives
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X-from: hexi-at-missouri.edu


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Email: hexi-at-missouri.edu Name: Xiaoqing He

Organization: Universtiy of Missouri

Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100%

Message: Hi all,

We are having issues with our Oxford EDS detector on our F30. With no
electron beam on, the dead time stayed at 100% no matter which
dispersion rate we choose. Initially we thought the misconnection
between Oxford and TEM may be responsible. But the issue persists even
after we reboot Microscope PC, Oxford x-stream processor, software. etc.

Any inputs are greatly appreciated!

Thanks.

Xiaoqing He

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From: vray-at-partbeamsystech.com
Date: Sat, 4 Feb 2017 07:32:13 -0600
Subject: [Microscopy] Re: viaWWW: Need Gatan 794 Camera Controller

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi John,

One Gatan 794 controller is listed on E*Bay and the seller is willing to
ship to Colombia - this is not free, but certainly less expensive then a
new camera:

http://www.ebay.com/itm/GATAN-Retractable-Multiscan-Camera-Controller-Model-794-/361863685735?hash=item5440c1a667:g:K5cAAOSw5cNYYejS#shpCntId

Look really hard for local people who do component level repair of
complex electronics - if the problem is in controller and not the
camera, then most likely it is repairable! You just need to find someone
capable of such repair and willing to do it for the money you are able
to pay...

Best Wishes,
Valery

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 2/3/2017 11:40 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: jwsandinod-at-unal.edu.co Name: John W. Sandino
}
} Organization: Universidad Nacional de Colombia
}
} Title-Subject: [Filtered] Need Gatan 794 Camera Control
}
} Message: Two years ago we received an old 794 retractable camera Gatan
} as donation from the Tribenberg Lab (closed).
} But two days ago the camera controller of this camera does not work any
} more. Today the gatan people confirm the news and told as that the
} solution would buy a new camera. However we don't have budget for that.
}
} We ask for some who could have a camara contoller that coud give us for
} free.
} I know that it is no the best petition but for the moment is the unique
} solution to work with our tecnai 20. one of the best microscopes in our
} country Colombia.
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From: vray-at-partbeamsystech.com
Date: Wed, 8 Feb 2017 11:38:15 -0600
Subject: [Microscopy] JEOL JEM 2100F installation/service manual?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended


1. Günter Resch (Nexperion, Vienna)
2. TipTemp
3. Extech RH520A temperature/humidity data logger
4. Vernier.com, Go Direct sensors

Hope this will be useful for the rest as well!

Cheers,
Yee Yan




On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:



Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.

Cheers,
Yee Yan, Tay
FACTS Lab
NTU
Singapore


==============================Original Headers==============================
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14, 31 -- From: YY YY {rongchigram79-at-yahoo.com.sg}
14, 31 -- Reply-To: YY YY {rongchigram79-at-yahoo.com.sg}
14, 31 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
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14, 31 -- Subject: Re: Temperature Logger to Recommend
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From stepnoah502-at-gmail.com Sun Feb 5 10:47:09 2017
Return-Path: {stepnoah502-at-gmail.com}
Received: from gmail.com ([61.76.233.68])
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id v15Gl6IX001296
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Message-ID: {F404FFFA.5BD98938-at-gmail.com}

http://www.practicaldesign.com/THUM/thum.html

I have used two of these for over a decade to monitor T/H in microscope rooms.
USB interface, so PC should be on, and data stored in a database.

Cheers,

Fred Monson

-----Original Message-----
X-from: rongchigram79-at-yahoo.com.sg [mailto:rongchigram79-at-yahoo.com.sg]
Sent: Saturday, February 04, 2017 10:59 AM
To: Monson, Frederick {FMonson-at-wcupa.edu}

Dear All, thank you very much for those who have kindly made some recommendations! I have summarized here the ones that have been recommended


1. Günter Resch (Nexperion, Vienna)
2. TipTemp
3. Extech RH520A temperature/humidity data logger
4. Vernier.com, Go Direct sensors

Hope this will be useful for the rest as well!

Cheers,
Yee Yan




On Thursday, 26 January 2017, 16:51, YY YY {rongchigram79-at-yahoo.com.sg} wrote:



Dear All, i would like to ask for your recommendation for a temperature logger. Probably a reasonable sensitivity, acuracy and precision and is able to monitor live using the TEM pc. I am not sure if such system will interfere any performance of the TEM. If you encounter such problem, is it possible to share your experience? I do know some logger has wifi/bluetooth capability, will this interfere the TEM performances well? whiCurrently we have a temperature logger but its not live and we have to extract the data out from time to time which is very troublesome.

Cheers,
Yee Yan, Tay
FACTS Lab
NTU
Singapore


==============================Original Headers==============================
14, 31 -- From rongchigram79-at-yahoo.com.sg Sat Feb 4 09:48:05 2017
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14, 31 -- From: YY YY {rongchigram79-at-yahoo.com.sg}
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From marilore504-at-gmail.com Mon Feb 6 18:14:02 2017
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Email: mikeh-at-ncimicro.com Name: mike hehr

Organization: NCI

Title-Subject: [Filtered] EM Sample Preparation Sales Position

Message: Scientific Instrument distributor looking for a Nano Technology
Professional to sell Electron Microscopy sample preparation equipment in
the Midwest. The ideal candidate will have experience in Electron
Micorscopy and knowledge of the various instruments used to perform the
sample prep. These instruments include: Scanning Electron Microscopes,
Ion Millers, Ultra Microtomes, High Pressure Freezers, Critical Point
Dryers, Coaters, Knife Makers and other.

This is a highly consultative sales process that requires extensive
knowledge with EM sample preparation but also the winning spirit to win
the sale and train users on the equipment / techniques for successful
preparation.

Sales Experience preferred, but not needed for the right candidate.

Salary, Commission, Benefits

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From ronaldfaust205-at-gmail.com Wed Feb 8 06:27:11 2017
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Message-ID: {CA32B257.17A4C6A8-at-gmail.com}

Dear Listers,

I am wondering if anyone may have installation/service manual for Jeol
JEM 2100F TEM, or any notes related to its installation you would be
willing to share. I have full operation manual with the instrument, but
need to move it.

Thank you very much beforehand,
--
Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

==============================Original Headers==============================
3, 32 -- From vray-at-partbeamsystech.com Wed Feb 8 11:38:14 2017
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3, 32 -- From: Valery Ray {vray-at-partbeamsystech.com}
3, 32 -- Subject: JEOL JEM 2100F installation/service manual?
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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Feb 2017 14:13:27 -0600
Subject: [Microscopy] viaWWW:EM Microwave Tissue Processing Workshop February 14th and 15th

Contents Retrieved from Microscopy Listserver Archives
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Email: gavramo-at-fas.harvard.edu Name: Gil Avramovich

Organization: Harvard University Department of Molecular and Cell Biology

Title-Subject: [Filtered] EM Microwave Tissue Processing Workshop

Message: Message: RSVP now for an advanced Microwave Tissue Processing
Workshop for applications in Electron Microscopy, which will be held on
the Cambridge campus of Harvard University, room 254 in NW Labs, 52
Oxford St, Cambridge MA 02138. Join us on February 14th and 15th, and
enjoy complimentary food and coffee, and enjoy learning about cutting
edge technology alongside the foremost experts in Electron Microscopy.
The workshop will cover:
ƒÞ Microwave Technology in Research, ƒÞ Sample Preparation for Microwave
Processing,
ƒÞ Microwave Processing for Electron Microscopy,
ƒÞ Electron Microscopy Polymerization, and
ƒÞ Alternative applications and associated kits. The workshop is totally
free, and spots are limited, so please send your RSVP to
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From: zaluzec-at-microscopy.com
Date: Wed, 8 Feb 2017 14:13:40 -0600
Subject: [Microscopy] viaWWW:Technical Sales Position: Mid Atlantic

Contents Retrieved from Microscopy Listserver Archives
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X-from: rms-at-angstrom.us

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Email: rms-at-angstrom.us Name: Bob Sommerville

Organization: Angstrom Scientific Inc

Title-Subject: [Filtered] Technical Sales Position: Mid Atlantic

Message: Position: Technical Sales Representative - Mid Atlantic US
Territory: Dependent on the candidate but will include MD, DE, DC, VA,
NJ & PA. Products: Sales and support of the following
products/companies: Hitachi: Bench-top Scanning Electron
Microscopes (SEM) and AFM Kleindiek: Nano-manipulators, Probe
Stations and Stages Leica: Electron Microscopy sample prep.
equipment
EMSIS TEM Cameras Deben Tensile Stages and Microscope
AccessoriesJ
V/Bruker Semi: X-ray Diffraction (XRD)


Position Description: This is an exciting position selling and
supporting a range of products in for a number of leading companies in
the electron microscopy and related markets. The ideal candidate will
preferably have a demonstrated track record of success (2-3 years)
selling technical products into the academic, research and industrial
and/or life sciences markets. Optical microscopy or SEM/TEM experience a
definite plus. Academic background should be at min. an associate’s
degree in science (BS or masters preferred). The successful candidate
will live in one of the above listed states. Angstrom Scientific Inc.
is a growing distributor of products targeted to the nanotechnology,
research, life science and semiconductor markets with exciting new and
novel technology. The recent addition of Leica with its impressive
portfolio of EM sample prep. equipment complements and strengthens our
position in this region. Angstrom Scientific Inc. offers competitive
salary and benefits, commission, car allowance and expenses. We are an
equal opportunity employer.
Interested candidates should submit their resume to: Bob
Sommerville, CEO, Angstrom Scientific Inc. e-mail: rms-at-angstrom.us

Keywords: Scanning Electron Microscope, SEM, TEM, EM Sample Preparation,
X-Ray, XRD, Nano-Manipulators, Probing.


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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Feb 2017 16:24:15 -0600
Subject: [Microscopy] viaWWW:Position open in Basel, Switzerland: Microscopy

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Hello,
It could be that you have different grounding points in use, and an electric potential between them causing a ground current being the issue. You might want to check that your ground lines are correctly wired.

The general recommendation is to use the frame of the TEM as the only grounding point for your TEM connected accessories, arranging a grounding bus at the frame and avoiding any ground loops between the accessories.
For instance, do not use the house grounding line feeding to the power socket which powers the EDX equipment and EDX computer. The house grounding line stays disconnected from this power socket. This socket's grounding (not the house grounding line) becomes linked to the frame of the TEM, instead. If I remember correctly, then Oxford ships an accordingly manipulated distributor with their equipment for easing such wiring. Don't forget to also connect the chassis of the EDX computer to your grounding point at the frame of the TEM.
Consult a certified electrician for assuring electrics security in your setup!

Best greetings from the EM-Labs of CIC biomaGUNE (Spain),
Marco Mller


}
} Email: hexi-at-missouri.edu Name: Xiaoqing He
}
} Organization: Universtiy of Missouri
}
} Title-Subject: [Filtered] Oxford INCA EDS detector dead time 100%
}
} Message: Hi all,
}
} We are having issues with our Oxford EDS detector on our F30. With no
} electron beam on, the dead time stayed at 100% no matter which
} dispersion rate we choose. Initially we thought the misconnection
} between Oxford and TEM may be responsible. But the issue persists even
} after we reboot Microscope PC, Oxford x-stream processor, software.
} etc.
}
} Any inputs are greatly appreciated!
}
} Thanks.
}
} Xiaoqing He


==============================Original Headers==============================
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6, 46 -- Subject: RE: Oxford INCA EDS detector dead time 100%
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From pedugjuf5-at-gmail.com Thu Feb 9 05:44:48 2017
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Email: thomas.horn-at-bsse.ethz.ch Name: Thomas Horn

Organization: ETH Zurich

Title-Subject: [Filtered] Position open in Basel, Switzerland:
Microscopy Engineer/Microscopist

Message: Dear all, we have an open position at the Single Cell Facility
, ETH, Department of Biosystems Science and Engineering (D-BSSE), Basel:
Microscopy Engineer / Microscopist (m/f)
Responsibilities are training and support of facility users. This
includes microscopy instrument quality control and their maintenance.
The microscopy engineer/microscopist will develop and implement
customized configurations and the setup of the experimental workflows of
experiments on the automated imaging systems. The successful candidate
will share responsibilities with our other microscopy expert and work
closely together with our software engineer for image analysis and data
management. This position is available for 2 years and located in Basel.
Requirements are a degree from a technical college or a university with
a proven experience in various advanced light microscopy and digital
imaging technologies. She/he will have extensive technical experience
with state-of-the-art light microscopy platforms such as confocal,
live-cell imaging and/or SPIM. Strong knowledge of advanced quantitative
imaging technologies as well as their underlying optical principles is
required. Scripting experience is a plus. The candidate should have
hands-on skills in microscopy component integration. This position
demands very good communication skills in English to interact with
scientists and students, and the skill to adapt to the service character
of the facility to successfully support scientific research and
operations at the D-BSSE. Besides having excellent technical abilities
in the field of light microscopy, the candidate must be an excellent
team player and possess the ability to work in a variety of scientific
areas. The Single Cell Facility (SCF) is a central scientific core
facility within the ETH Zurich, Department of Biosystems Science and
Engineering (D-BSSE), located in Basel. The SCF serves the department by
providing technology and support in advanced (light-sheet, automated
wide field, confocal) microscopy and flow cytometry.
For further information about the position, please contact Dr. Thomas
Horn, by email: thomas.horn-at-bsse.ethz.ch (no applications).

Applications are only accepted online, please use the “Apply now” button
at the link below. Please address it to: ETH Zurich, Roland Munz, Human
Resources, CH-8092 Zurich.

https://apply.refline.ch/845721/5177/pub/en/index.html

Further information on our facility: https://www.bsse.ethz.ch/scf

Best regards, Thomas Horn.
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From: Colin.Veitch-at-csiro.au
Date: Sun, 12 Feb 2017 20:28:37 -0600
Subject: [Microscopy] Gauss Maus manual

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] EELS Training School - April 2017, California

Message: EELS & EFTEM Analysis Training School

April 4-7, 2017, Gatan R&D Headquarters, Pleasanton , CA

Electron energy loss spectroscopy (EELS) is a powerful technique that
provides both compositional and chemical information from sub-nanometer
areas in the sample. As a course attendee, you will learn best practices
to set up and optimize your EELS hardware and experimental protocols so
you can capture and extract the maximum amount of compositional and
chemical information from your TEM samples. Topics include:

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From shkrsugi29-at-gmail.com Sun Feb 12 11:00:56 2017
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Message-ID: {27532BAF.4AEABBE4-at-gmail.com}

Hi,

We have an old Gauss Maus (Dindima Group/Arlunya) , a hand held device for measuring magnetic fields. We need to work out the magnitude and direction of some stray fields that have just appeared (intermittently of course) in our lab but unfortunately have lost the manual. I have searched on the internet but couldn't find a manual there. If anyone has a copy of the manual they could scan and email to me or, send to me so that I can scan it and send it back it would be much appreciated.

Thank you very much.

Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory
CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057
Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx


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From: Colin.Veitch-at-csiro.au
Date: Sun, 12 Feb 2017 23:10:22 -0600
Subject: [Microscopy] Gauss Maus manual found.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

With many thanks to Richard Easingwood of Otago University in the "Land of the Long White Cloud" the manual for the Gauss Maus has been found.

Cheers


Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory
CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057
Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx


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From: bicarbaj-at-mtholyoke.edu
Date: Tue, 14 Feb 2017 10:42:06 -0600
Subject: [Microscopy] TEM: LaB6 cathodes--status?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello everyone,

Last spring, a thread was started on the quality/longevity of newer
LaB6 cathodes. I looked back through the e-mails and it seemed like
the last thing that was mentioned was something about ongoing
discussions with the vendors.

I have some grant money left that I need to spend soon and was
thinking of switching our CM100 over from W to LaB6. I worry, however,
that it may not be worth it since it seemed like others were having
problems with the longevity of the cathodes.

I did contact the original person who started the thread on this
listserv but have yet to receive a reply. Does anyone know what the
status of the cathodes is?

Thank you,
-Blanca
-----------------------------------------
Blanca Carbajal Gonzalez, M.S.
Director of Microscopy
Science Center
50 College St
Mount Holyoke College
Clapp Laboratory 123
Office: 413-538-3118
Cell: 559-905-7138
bicarbaj-at-mtholyoke.edu
MHC Microscopy

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From: PhillipsT-at-missouri.edu
Date: Fri, 17 Feb 2017 12:05:14 -0600
Subject: [Microscopy] Ultracut S ultramicrotome repair question

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Email: fengxia.liang-at-med.nyu.edu Name: Alice Liang

Organization: New York University Langone Medical Center

Title-Subject: [Filtered] CryoEM manager-NYU

Message: Cryo EM Manager Position at NYU Langone School of Medicine

NYU Langone School of Medicine is seeking a cryo-EM manager to oversee
the operation of our expanding, state-of-the- art electron microscopy
facility. The facility will include Titan Krios and Talos Arctica
microscopes, both with direct detectors, for single particle EM and cryo
electron tomography. The facility will be staffed by 3 team members,
including the manager (this position) and two junior associates. The
facility manager will be responsible for oversight and maintenance of
these microscopes as well as training and assisting users, who will be
primarily from the NYU research community. The manager will interface
with our High Performance Computing team to ensure a seamless interface
between data collection and data processing. The manager will also have
the opportunity to, and will be encouraged to interact with cryo EM
experts in the New York area and to attend key EM conferences (e.g. EM
Gordon Conference) in order to integrate NYU with the greater EM
community, and to implement cutting-edge techniques and technologies
into our facility.

Responsibilities (together with 2 other team members):
Maintain Talos Arctica and Krios microscopes. This duty requires
interfacing with FEI engineers to ensure optimal performance of the
microscopes.
Maintain ancillary, cryo-EM related equipment such as Vitrobot, glow
discharger etc.
Load samples and align microscopes prior to data collection
Train and oversee users for data collection Evaluate quality of data
obtained by users
Interface and collaborate with High Performance Computing team, who will
assist with computational needs for optimal data collection, storage and
data processing
Interface and collaborate with Microscopy Core, which houses electron
microscopes for preliminary screening of samples
Implement data collection strategies that will benefit our user
community Maintain accurate logs for microscope performance and usage

Key element of the job:
This facility will be optimized for high throughput data collection.
Developing a pipeline that fits well with our users will be a key part
of this job. Although the manager is expected to consult with faculty
supervisors, he/she will be given flexibility and responsibility to
develop a pipeline that generates high quality data in an efficient manner.

Requirements:
PhD in structural biology, biochemistry or related field
At least 2 years of cryo EM experience, with expertise in handling
samples, using microscopes and evaluating images
Excellent communication and interpersonal skills
Knowledge of the principles of transmission electron microscopy
General understanding of single particle cryo EM workflow; experience in
cryo electron tomography is a plus Special consideration will be given
to candidates with management experience or a record of achievement in
collaborative, team-driven environments

The Ideal Candidate:
In addition to meeting all the requirements above, the ideal candidate
will also bring enthusiasm and passion to developing our new facility.
We are looking for a colleague who will be excited about interacting
with researchers at all levels and will consistently strive to maintain
a state-of-the-art facility. The EM manager will be an key component of
our new cryo EM initiative, and as such, will play a crucial role in
shaping the new facility. This job is an excellent opportunity for an
electron microscopist who is interested in a leadership role within the
vibrant research community at NYU School of Medicine.

Salary will be competitive and job title will depend on qualifications
of the candidate.

To apply for this position, please email cryoemnyu-at-gmail.com and
include: 1) Cover letter
2) CV
3) Contact information for 3 references

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From stepnoah502-at-gmail.com Wed Feb 15 13:56:35 2017
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Message-ID: {FB6D4791.5BCEEE94-at-gmail.com}

We need to remove the top of our Leica Ultracut S ultramicrotome to check something on the electrical circuit for the overhead light source. I am talking about the top plate that carries the binocular microscope that allows you to view the specimen and cutting action.

It looks like there is a screw at the very back of this plate that can be seen from the underside when the plate is rotated 90 degrees. I am guessing that one removes that screw and then you can used the normal hand driven gear drive to move the carrier forward until it is free of the underlying dovetail. Can anyone confirm this. Additionally, where does one disconnect the electrical to the fluorescent light source? Will I see a plug once I remove this screw and move the carrier forward?

Thanks, Tom

Thomas E. Phillips, Ph.D
Curator's Distinguished Teaching Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



Thomas E. Phillips, Ph.D
Curator's Distinguished Teaching Professor of Biological Sciences
Director, Molecular Cytology Core
2 Tucker Hall
University of Missouri
Columbia, MO 65211-7400
573-882-4712 (office)
573-882-0123 (fax)
phillipst-at-missouri.edu

http://www.biology.missouri.edu/faculty/phillips.html
http://microscopy.missouri.edu



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11, 38 -- Subject: Ultracut S ultramicrotome repair question
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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Feb 2017 11:42:37 -0600
Subject: [Microscopy] viaWWW:FIB STEM EDX

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X-from: roger.ristau-at-uconn.edu

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Email: roger.ristau-at-uconn.edu Name: Roger Ristau

Organization: University of Connecticut

Title-Subject: [Filtered] FIB STEM EDX

Message: A question for anyone with FIB-STEM-EDX system:
Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg
rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the
lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by
the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is
replaced by a Al peak from the stage area situated below the STEM position.

Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the
thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector.
I am presuming it may have something to do with the larger collection angle of the SDD. I have
confirmed with EDAX that the detector is installed correctly,the collimator is in position and the
system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage'
position.)

My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to
absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable
as that makes STEM imaging impossible.

Has anyone else encountered this with a similar system?

Cheers
Roger Ristau
Univ of Connecticut

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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Feb 2017 11:43:23 -0600
Subject: [Microscopy] rviaWWW:EDS detector shutter issue

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Title-Subject: [Filtered] EDS detector shutter issue.

Message: Hi Listeners,
I am using Tecnai TEM equiped with Oxford EDS. Whenever I open the shutter to start the EDS
analysis, it's not getting open, it shows overload, while it was not even opened for the days. Could
you guys help me to fix this issue.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 18 Feb 2017 11:51:19 -0600
Subject: [Microscopy] viaWWW:Service Engineer for FEI Tecnai

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Email: steve.sem-at-icloud.com
Name: Steve Perry

Title-Subject: [Filtered] Service Engineer for FEI Tecnai

Message: I would like to hear from a service engineer who is capable and available to perform
service work on our Tecnai F30.

Located on the West coast USA. Travel and accommodations can be provided if required.

Reply in confidence,



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From: vray-at-partbeamsystech.com
Date: Sat, 18 Feb 2017 13:14:24 -0600
Subject: [Microscopy] Re: viaWWW:FIB STEM EDX

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Hi Roger,

For a quick fix - get Beryllium foil of adequate thickness (0.5mm is
widely available) and cover areas *behind* the retractable STEM detector
exposed to e-beam transmitted through the sample. You could get STEM
image with extended detector and EDS + Se image when detector is retracted.

It is possible that once main Al peak is dealt with, some additional
stray signal generated by electrons scattered from/through the sample
could become detectable - find out where they come from and shield with
Be foil.

Be is considered "hazmat," but that is when it is in
dust/powder/fume/solution form which can be ingested, inhaled, or
absorbed into the body. If you do not drill/sand/machine Be foil but
only attach it by conductive vacuum epoxy it should be safe enough to
stay there. Lots of Be windows were (and still are) out there installed
in SEMs.

If Beryllium is too much of a scare, use Aquadag paint of adequate
thickness to cover area behind STEM detector. Best would be to
manufacture a shield from some kind of metal, coat it with Aquadag,
anneal in vacuum oven -at- 300 degrees, and then mount into SEM chamber.

Best Wishes :)
Valery

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 2/18/2017 12:44 PM, microscopy.listserver-at-gmail.com wrote:
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}
} Email: roger.ristau-at-uconn.edu Name: Roger Ristau
}
} Organization: University of Connecticut
}
} Title-Subject: [Filtered] FIB STEM EDX
}
} Message: A question for anyone with FIB-STEM-EDX system:
} Our FEI Helios 460F1 has single-sample Flip Stage (the 'turret' type Flip Stage with 180 deg
} rotation) and STEM detector for imaging TEM liftouts in situ. When attempting to acquire EDX of the
} lifout, mounted on the Flip Stage, using EDAX Octane Plus SDD EDX, the spectrum is overwhelmed by
} the Si peak from the STEM detector itself. If the STEM detector is retracted, the Si peak is
} replaced by a Al peak from the stage area situated below the STEM position.
}
} Obviously the EDX detector is 'seeing' the X-rays generated by the e-beam that passes through the
} thin liftout sample. However, this problem did not occur in our older Strata FIB with SiLi detector.
} I am presuming it may have something to do with the larger collection angle of the SDD. I have
} confirmed with EDAX that the detector is installed correctly,the collimator is in position and the
} system is operating normally. (There are no problems doing EDX with the sample in the 'bulk stage'
} position.)
}
} My temporary solution is to affix a strip of carbon tape to the 'underside' of the Flip Stage to
} absorb the transmitted e-beam, eliminating the spurious Al peak, but this is obviously undesirable
} as that makes STEM imaging impossible.
}
} Has anyone else encountered this with a similar system?
}
} Cheers
} Roger Ristau
} Univ of Connecticut
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8, 35 -- References: {201702181744.v1IHi58Q032489-at-microscopy.com}
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From: dsampson-at-ucsc.edu
Date: Tue, 21 Feb 2017 10:31:38 -0600
Subject: [Microscopy] Free ARL-SEMQ uProbe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

I have a vintage 1980 ARL-SEMQ microprobe, mostly working, that needs a
home. This was the deluxe model with all of the bells and whistles
available at the time (including CL imaging), and it was updated to the
Advanced Microbeam automation system in the 90's. If you have any
interest please contact me. I need to get it out of the lab soon to make
room for new equipment. I also have nearly an entire second one in
spares. If nobody takes it then it all goes to scrap.

Thanks,

Dan

--
****************************************************************************
A man who lies to himself, and believes his own lies, becomes unable to
recognize truth, either in himself or in anyone else, and he ends up losing
respect for himself and for others. -Fyodor Dostoevsky, novelist (1821-1881)
****************************************************************************
Daniel E. Sampson
Instrument Engineer
A232 Earth and Marine Sciences
University of California
Santa Cruz, CA 95064
dsampson-at-ucsc.edu
(831) 459-4992 office
(831) 359-9075 cell
(831) 459-3074 FAX
****************************************************


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From: microscopy.listserver-at-gmail.com
Date: Tue, 21 Feb 2017 21:06:15 -0600
Subject: [Microscopy] viaWWW:Hitachi SU6600 FE-SEM Constant Beam Drift?

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Email: ibarke2-at-uwo.ca Name: Ivan Barker

Organization: Western University

Title-Subject: [Filtered] Hitachi SU6600 FE-SEM Constant Beam Drift?

Message: Hi all,

A quick question that I hope someone could help me with.

In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of
beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and
maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but
eventually, the range for that tops out, and we cannot get crisp images. This occurs with well
grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin
sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I
originally thought it was due to my grounding, but it used to be fine, and no matter what I do the
images still drift.

However, if I put things into variable pressure mode it is fine.

It has been a while since we've done a "bakeout", so I'm just wondering if it could be something
related to tip alignment, or if there are particles in the column? What can I do to check the
instrument? I just worry there's something going on with the tip. How can I check that?

Thank you in advance for any info and tips!
- Ivan

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From: microscopy.listserver-at-gmail.com
Date: Tue, 21 Feb 2017 21:07:04 -0600
Subject: [Microscopy] viaWWW:3D modeling software

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Email: krueger.eugene-at-mayo.edu Name: Eugene Krueger

Organization: Mayo Clinic, Rochester, MN

Title-Subject: [Filtered] 3D modeling software

Message: Hello microscopists and vendors,
I was wondering what commercial software packages people are using for 3D modeling of confocal
Z-stacks.I can generate beautifully detailed Z-stacks, but would like to be able to make models from
the data that I could use in other applications. Specifically, I would like to generate 3D models
that I could manipulate and use in movies and presentations. Any information would be appreciated,
and vendors are encouraged to reply.

Thank you,
-Eugene

Eugene Krueger
Sr. Research Tech II
Mayo Clinic

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From: vray-at-partbeamsystech.com
Date: Wed, 22 Feb 2017 00:04:35 -0600
Subject: [Microscopy] Re: viaWWW:Hitachi SU6600 FE-SEM Constant Beam Drift?

Contents Retrieved from Microscopy Listserver Archives
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Hi Ivan,

Although it is hard to diagnose remotely, chances are that problem is
not with the gun. If drift was related to electron source, then most
likely it would be appearing similarly in both HV and variable pressure
modes.

There is a good chance that there is some kind of dielectric
contamination, particle, or a piece of fiber stuck at the bottom of
objective lens. It is getting charged during high-vac operation and
deflects/astigmates the beam. When you switch to variable pressure mode
then ADAPT is inserted, shielding contamination from the beam, thus
preventing the drift. You would need to open specimen chamber and
carefully inspect bottom of the column and opening in the polepiece with
mirror and a good flashlight, and try to look inside of the polepiece
opening. Also take a look on outside of the insertable variable aperture
for any traces of contamination or particles. Make sure that in
high-vacuum mode ADAPT is fully retracted. Check resistance from
polepiece to ground with ohmmeter, however strange this sounds. Also
check grounding of your stage - there is some possibility that stage is
electrically floating and thus getting charged in High Vac mode.

Best Wishes,

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
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Web: www.freudlabs.com

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} Email: ibarke2-at-uwo.ca Name: Ivan Barker
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} Organization: Western University
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} Title-Subject: [Filtered] Hitachi SU6600 FE-SEM Constant Beam Drift?
}
} Message: Hi all,
}
} A quick question that I hope someone could help me with.
}
} In our daily routine analyses with our Hitachi SU6600 FE-SEM, we are getting a large component of
} beam drift. As in, the image seems to migrate while doing an analysis, leading to blurry images and
} maps. When the SEM initially turns out, we can correct for this drift with the stigmators, but
} eventually, the range for that tops out, and we cannot get crisp images. This occurs with well
} grounded samples, while doing routine imaging or EDS analyses. We typically analyze geological thin
} sections, but we coat with carbon and ground with either carbon or silver paint, or copper tape. I
} originally thought it was due to my grounding, but it used to be fine, and no matter what I do the
} images still drift.
}
} However, if I put things into variable pressure mode it is fine.
}
} It has been a while since we've done a "bakeout", so I'm just wondering if it could be something
} related to tip alignment, or if there are particles in the column? What can I do to check the
} instrument? I just worry there's something going on with the tip. How can I check that?
}
} Thank you in advance for any info and tips!
} - Ivan
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6, 35 -- From vray-at-partbeamsystech.com Wed Feb 22 00:04:35 2017
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From: John.Mardinly-at-asu.edu
Date: Fri, 24 Feb 2017 10:21:40 -0600
Subject: [Microscopy] Mildred Dresselhaus died on Monday in Cambridge, Mass. She was 86.

Contents Retrieved from Microscopy Listserver Archives
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We had a similar problem with a Hitachi S-2460N many years ago. I don't know how it compares to the 6600.

We had let the PM schedule lapse and got similar drifting. The engineer came in and cleaned out the liner tube, as I recall. It had built up a deposit on one side that took on a charge in hivac mode. Once it was cleaned, things were back to normal with no drift in hivac or lovac mode.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: vray-at-partbeamsystech.com [mailto:vray-at-partbeamsystech.com]
Sent: Wednesday, February 22, 2017 12:06 AM
To: Straszheim, Warren E [BIOTC]

Mildred Dresselhaus, a professor emerita at the Massachusetts Institute of Technology whose research into the fundamental properties of carbon helped transform it into the superstar of modern materials science and the nanotechnology industry, died on Monday in Cambridge, Mass. She was 86.

A. John Mardinly, Ph.D., P.E.
Retired Principal Materials Nanoanalysis Engineer










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From: amit.welcomes.u-at-gmail.com
Date: Sat, 25 Feb 2017 04:39:52 -0600
Subject: [Microscopy] Is this paper available anywhere?

Contents Retrieved from Microscopy Listserver Archives
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Rose H (1990) Outline of a spherically corrected semi-aplanatic
medium-voltage TEM. Optik 85: 19–24.

I cannot find it anywhere on internet. Optik journal somehow only show
issues till 112. Is it obtainable from anywhere?


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From: microscopy.listserver-at-gmail.com
Date: Sat, 25 Feb 2017 09:49:55 -0600
Subject: [Microscopy] viaWWW:Carbon Grains on Formvar Grids or Carbon Film

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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] Carbon Grains on Formvar Grids or Carbon Film

Message: Sometimes it is very difficult to tell between what is sample and what is carbon grain
(either grids coated with compressed carbon) or the carbon on Formvar grids. Can anyone shed light
on this when one does protein analysis?

We are working at 100kV and 140Kx mag.

Thanks!

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From: rok210-at-lehigh.edu
Date: Mon, 27 Feb 2017 10:23:13 -0600
Subject: [Microscopy] Re: viaWWW:Carbon Grains on Formvar Grids or Carbon Film

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Hi Vickie,

carbon films come in different grades one of which is 'ultra-thin' and
has areas down to 3nm thick according to the publicity in catalogs.
I've never done protein analysis, but you need a supporting substrate
and one that is amorphous, so try and look in the holes since they may
be covered with thin carbon, the thinner the better.

I have bought some silicon nitride supports that are thin and they can
be plasma cleaned between uses, but they are a bit pricey.

Good luck
Rob

--
Robert Keyse, DPhil
Research Scientist
Lehigh University

5, East Packer Avenue,
Whitaker Laboratory
Bethlehem,
PA 18015-3194

Office (610)758-3465
Fax (610)758-4244


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From: bicarbaj-at-mtholyoke.edu
Date: Mon, 27 Feb 2017 13:49:17 -0600
Subject: [Microscopy] NESM Spring Meeting March 2nd

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Hello everyone,

The New England Society for Microscopy's Spring Meeting this year will
be held at GE Healthcare in Marlborough, MA this week, March 2nd from
5pm-8pm.

For more information and to register, please visit our website:
http://nesmicroscopy.org/upcoming-meetings/

We hope to see you there!

Cheers,
-NESM board

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Mar 2017 06:10:39 -0600
Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers

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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell

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Title-Subject: [Filtered] Ultramicrotomes and gridstainers

Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes
(Ultracut E) and our aged LKB Ultrostainer. We are looking at
- RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or

- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.

I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.

Many thanks

Allan

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Mar 2017 06:49:17 -0600
Subject: [Microscopy] viaWWW: Ultramicrotomes and gridstainers

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Email: duraine-at-bcm.edu Name: Lita Duraine

Organization: Howard Hughes Medical Institute

Title-Subject: [Filtered] Re: viaWWW:Ultramicrotomes and gridstainers

Message: Hi Allan,

I totally support the Leica Ultracut EM UC7 microtome. We bought one in 2013 and it has worked
efficiently ever since. It is very user friendly and the program settings are easy to manage.
Hardly any break-in time. The thickness settings stay consistent. You also have a wide latitude
for cutting speeds. There are several light settings especially helpful when I am hand trimming a
block and then want to go immediately to cutting thins. This instrument is so accurate that it can
cut silver sections effortlessly even on the 11th floor of our building! We loved it so much that
we bought a second one two years later when we expanded our lab.
Customer service is another reason why I use Leica. We had a small static shock occurring in the
new room where we put the microtome. Not sure what was causing it, I called Leica. Immediately,
they sent an engineer to our location in Houston and checked everything on the UC7. Several issues
were found within the room actually, and everything turned out great. The UC7 is a workhorse, and
never skips a beat. I highly recommend the Leica UC7 Ultramicrotome.

Lita Duraine
Certified Electron Microscopist
Howard Hughes Medical Institute
Houston, TX 77030


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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Mar 2017 06:50:14 -0600
Subject: [Microscopy] viaWWW:The Materials Ultramicrotomy Workshop

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Title-Subject: [Filtered] The Materials Ultramicrotomy Workshop

Message: Three days of hands-on training for technicians, researchers, and students who want to
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EMS Microscopy Academy
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Instructors:
Helmut Gnaegi, Diatome Ltd., Switzerland Robert Ranner, Leica, Austria
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From: ph2-at-sprynet.com
Date: Thu, 2 Mar 2017 18:55:26 -0600
Subject: [Microscopy] Inter/Micro 2017, Chicago, IL June 12-16, 2017

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FYI


Inter/Micro 2017
69th Annual International Microscopy Conference
June 12 - 16, 2017
at
McCrone Research Institute, 2820 S. Michigan Avenue, Chicago, IL 60616

Call for Papers: Speaker Presentations

June 12-14: McCrone Research Institute cordially invites you to give a
presentation of your microscopy research at the 69th annual Inter/Micro
conference in Chicago. Join professional and amateur microscopists from
around the world as they present new research on techniques and
instrumentation, environmental and industrial microscopy, and chemical and
forensic microscopy. Speakers receive at $50 registration discount. The
abstract submission deadline is March 17, 2017. View abstract submission
guidelines at:

https://www.mcri.org/v/1199/Call-for-Papers-Abstract-Submission-Guidelines


Tony
..........................
Andrew Anthony "Tony" Havics, CIH, PE
Environmental, Health & Safety, Microscopy, Materials Science & Forensic
Engineering
pH2, LLC
5250 E US Highway 36, Suite 830
Avon, IN 46123
(317) 718-7020 Office
(317) 718-7038 Fax
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aahavics-at-pH2LLC.com
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From: jthompso-at-csusb.edu
Date: Thu, 2 Mar 2017 19:06:26 -0600
Subject: [Microscopy] SEM Need help in understanding loss of image generation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.

We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.

Any suggestions as to what the problem is? I can send images off-line.


Jeffrey Thompson, Ph.D.
Professor of Biology
California State University
5500 University Pkwy
San Bernardino, CA 92407
909-537-5315



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7, 49 -- Subject: SEM Need help in understanding loss of image generation
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From: vray-at-partbeamsystech.com
Date: Thu, 2 Mar 2017 20:48:40 -0600
Subject: [Microscopy] Re: SEM Need help in understanding loss of image

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Jeffrey,

Please feel free to send or post images to look at, but most likely you
have a simple deflection problem. Remember that in SEM lenses are only
used to form the beam, but magnification (and formation of image) comes
from ratio between area physicall scanned by electron beam on surface of
the sample and area of the CRT screen (or digital image) representing
the image. If you see only vertical lines then chances are that primary
electron beam is not scanned in Y direction over the sample, so in every
line of image you are seeing information from the same exact line
physically scanned by electron beam on the sample. Find a local tech who
knows how to use oscilloscope and able to figure out amplifiers driving
inductive loads (scan coils)....

Valery Ray
www.linkedin.com/in/valeryray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-296-5063 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 3/2/2017 8:07 PM, jthompso-at-csusb.edu wrote:
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} Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.
}
} We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.
}
} Any suggestions as to what the problem is? I can send images off-line.
}
}
} Jeffrey Thompson, Ph.D.
} Professor of Biology
} California State University
} 5500 University Pkwy
} San Bernardino, CA 92407
} 909-537-5315
}
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} 7, 49 -- Subject: SEM Need help in understanding loss of image generation
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4, 35 -- Subject: Re: [Microscopy] SEM Need help in understanding loss of image
4, 35 -- generation
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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Mar 2017 23:06:20 -0600
Subject: [Microscopy] viaWWW:Cryo-in-the-Sun Workshop

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From: Nicolas.Stephant-at-univ-nantes.fr
Date: Fri, 3 Mar 2017 02:02:11 -0600
Subject: [Microscopy] Re: SEM Need help in understanding loss of image generation

Contents Retrieved from Microscopy Listserver Archives
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Hi Jeffrey,
I'm agree with Valery, a deflection circuit is faulty. A deflection
problem is also possible from the screen circuit. This can be check by
connecting the video output on an external monitor. This microscope has
probably two stages of deflectors to scan the beam and each of them set
with X and Y deflection coils. The most common problem is coming from
power transistor on horizontal circuit. This component is more
sollicited because the scan speed horizontal is faster than the
vertical. This electronic is sensitive to temperature of the room and of
the cooling water. The fastest is the speed the more those transistors
are working, if you can set your microscope to slow scan mode when you
don't use, it's safety for deflection circuit. High magnification
position is also good.

Nicolas STEPHANT

Universit de Nantes
Institut Jean Rouxel
Service de microscopie lectronique balayage et microanalyse
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Le 03/03/2017 02:21, jthompso-at-csusb.edu a crit :
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} Our Hitachi S-2700 SEM will no longer produce an image. We can only see a series of vertical lines of varying intensity with both secondary electron and backscatter detectors. We do have an electron beam - there are changes in intensity of the vertical lines during adjustment of filament current, beam tilt, beam horizon, changes in the lines if we move the specimen. We do lose the image if the HV is turned off on the secondary electron detector. A very similar pattern is seen with the backscatter detector, so it does not appear to be a detector issue.
}
} We have exchanged circuit boards and even the entire column (!) from a second identical scope used for parts. A commercial technician was unable to correct the problem. His best guess is that one or more of the lenses are not functioning.
}
} Any suggestions as to what the problem is? I can send images off-line.
}
}
} Jeffrey Thompson, Ph.D.
} Professor of Biology
} California State University
} 5500 University Pkwy
} San Bernardino, CA 92407
} 909-537-5315
}
}
}
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} 7, 49 -- Subject: SEM Need help in understanding loss of image generation
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7, 26 -- From Nicolas.Stephant-at-univ-nantes.fr Fri Mar 3 02:02:10 2017
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From: protrain-at-emcourses.com
Date: Sat, 4 Mar 2017 03:46:47 -0600
Subject: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
a friend of mine is interested in buying a SEM with a LaB6 emitter, for
imaging and, above all, analytical applications: EDS and WDS. However,
he was told that LaB6 emitters are not a good choice for analytical
applications in a SEM, mainly due to stability issues, which - if I got
it correctly - would require a long wait time before reliable spectra
could be acquired.
I have no experience with such a machine, so I can't advice him about
this. Maybe some of you can help me.
Thank you in advance.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

==============================Original Headers==============================
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6, 46 -- From: Davide Cristofori {dcristofori-at-unive.it}
6, 46 -- To: microscopy-at-microscopy.com
6, 46 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS?
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From medinaluca1-at-gmail.com Fri Mar 3 16:42:07 2017
Return-Path: {medinaluca1-at-gmail.com}
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Message-ID: {8F6BD8DD.8098AF46-at-gmail.com}

Hi

I know of no problems with LaB6 emitters with regard to stability, provide
that the vacuum level in the electron gun is suitable for their use. Could
you be confusing the instability problem with cold field emitter
instruments, where their natural emission is prone to early and late
instabilities during long operating session.
Regards

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com



-----Original Message-----
X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it]
Sent: 03 March 2017 16:24
To: protrain-at-emcourses.com

Dear Listers,
a friend of mine is interested in buying a SEM with a LaB6 emitter, for
imaging and, above all, analytical applications: EDS and WDS. However, he
was told that LaB6 emitters are not a good choice for analytical
applications in a SEM, mainly due to stability issues, which - if I got it
correctly - would require a long wait time before reliable spectra could be
acquired.
I have no experience with such a machine, so I can't advice him about this.
Maybe some of you can help me.
Thank you in advance.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e Dipartimento di Sc.
Molecolari e Nanosistemi Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~
~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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EDS and WDS?
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From: microscopy.listserver-at-gmail.com
Date: Sat, 4 Mar 2017 07:35:26 -0600
Subject: [Microscopy] viaWWW:Northern California Society for Microscopy Meeting March 16

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Email: roseann.csencsits-at-schafercorp.com Name: Roseann Csencsits

Organization: Schafer Laboratory

Title-Subject: [Filtered] Northern California Society for Microscopy Meeting March 16

Message: MSA Fellow and Featured speaker: Professor William Landis

"A possible role in vertebrate mineralization for the small, non-collagenous protein, osteocalcin,
as determined by immunocytochemistry"

Lecture includes: standard and high voltage TEM, computer simulation, immunocytochemistry, and
nucleation and growth of Hydroxyapatite

TEM, biology, and materials science - 3 in one! For what more could one ask?

Hosted by EAG, 810 Kifer Road, Sunnyvale, CA
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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Mar 2017 07:14:33 -0600
Subject: [Microscopy] viaWWW:Do you know about these electron dese bodies in ER?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear List
I am looking for SEM images of the various types/states of human white blood
cells. Does anybody know any accessible source of such images that is
scientifically sound?
Thanks for your time
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************





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5, 20 -- From eikonika-at-otenet.gr Sun Mar 5 01:52:51 2017
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5, 20 -- Subject: leucocyte types in SEM
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From donahenr845-at-gmail.com Mon Mar 6 03:48:07 2017
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Email: kuryu-at-rockefeller.edu Name: Hiro Uryu

Organization: rockefeller university

Title-Subject: [Filtered] Do you know about these electon dese bodies in ER?

Message: Dear list,

I was searching for a significance of electron dense bodies that appeared to be spherical and
located inside of ER. These may be heavily electron dense with or without an electron opaque core.
So far I found one image in the following link, indicting a type of structured that I have seen.
http://classes.kumc.edu/som/CellBiology/organelles/smoother/index.html
Does anyone have an idea what they might be and what the biological significance might be associated
with? I would love to hear your understanding of these dense bodies. I would be happy to share my
image if you would connect with me off site. Many thanks,

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From: nizets2-at-yahoo.com
Date: Tue, 7 Mar 2017 07:20:34 -0600
Subject: [Microscopy] Re: viaWWW:Ultramicrotomes and gridstainers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Allan!

We use a Leica EM UC6 for 12 years now, it is solid as a rock and precise like a swiss clock.
I just had to put one drop of oil in the gears mechanism after 10 years (3 screws to unscrew, I was able to do it myself!), which I accomplished very bravely :-D
Now I am troubled because I don't know anymore if the most loyal companion of men is the dog or a Leica ultramicrotome!

Regards,
Stephane



--------------------------------------------
On Wed, 3/1/17, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:

Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers
To: nizets2-at-yahoo.com
Date: Wednesday, March 1, 2017, 1:16 PM




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Email: allan.mitchell-at-otago.ac.nz
Name: Allan Mitchell

Organization: Otago centre for Electron Microscopy,
University of Otago

Title-Subject: [Filtered] Ultramicrotomes and gridstainers

Message: We are in the process of trying to obtain funding
to replace one of our ultramicrotomes
(Ultracut E) and our aged LKB Ultrostainer.  We are
looking at
- RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler
QG-3100 TEM Stainer, or

- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20
grid-stainer.

I would be keen to hear of peoples experiences with any of
these instruments, positives /negatives.

Many thanks

Allan

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From: ahk-at-bu.edu
Date: Wed, 8 Mar 2017 14:13:50 -0600
Subject: [Microscopy] JEOL 6100 SEM available

Contents Retrieved from Microscopy Listserver Archives
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Hello all:

We are in process of decommissioning our JEOL 6100 ( 25 yo), and it will be removed from lab in two weeks to make room for another equipment. The tool was last used over a year ago in good working condition, and had been under PM contract since new. It has an Oxford EDS (ISIS) unit, IR chamber camera, and specimen current meter on it. Please contact me if you are interested in taking it as a whole, or parts of it. You will need to pay for the packing and shipping of the tool from Boston to your location. Thanks.

Anlee Krupp
Laboratory Manager
Precision Measurement Laboratory
Boston University Photonics Center
8 Saint Mary's Street, Boston, MA 02215
Phone: (617) 353-9044
Email: ahk-at-bu.edu



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From: microscopy.listserver-at-gmail.com
Date: 29th March 2017
Subject: [Microscopy] viaWWW:Free Webinar - Revealing Cellular Dynamics with Millisecond

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Email: lon.nelson-at-leica-microsystems.com Name: LON NELSON

Organization: Leica Microsystems

Title-Subject: [Filtered] Free Webinar - Revealing Cellular Dynamics with Millisecond Precision
*Commercial Posting*

Message: **Commercial Posting**

Leica Microsystems and BitesizeBio have organized a free web-seminar in March that may be of
interest to you:

Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron
Micrographs in Motion Picture of Neural Communication


Presenters:
Dr. Shigeki Watanabe
Johns Hopkins School of Medicine

Dr. Frédéric Leroux
Leica Microsystems

What if you can dissect the cellular dynamics with millisecond precision?
What if you can unravel the morphological transformation of a neuron millisecond by millisecond
using electron microscopy?
Could this be even possible?

In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make
this possible.
We will discuss how to implement electrical stimulation and why it is superior to light stimulation.
We will also discuss the importance of sample processing and the challenges you would face while
freezing different types of samples.

Very best regards,

Lon Nelson
Director of Sales – Microscopy
lon.nelson-at-leica-microsystems.com
M +1 224-628-2467 | F +1 847-607-3160
Leica Microsystems, Inc.
1700 Leider Ln | Buffalo Grove, IL 60089 (USA)


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From: dcristofori-at-unive.it
Date: Fri, 10 Mar 2017 09:05:22 -0600
Subject: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating

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Dear Listers,
thanks to all of you who answered my question.
LaB6 emitters are unanimously considered stable, yet some of you pointed
out that the filament has to be kept heated in order to assure a stable
emission, even when not working, e.g. overnight.

I'd like to figure out how common is this approach, and the life of LaB6
emitters operated in this way. It would be very interesting and useful
to know your experience also about this point, if you want to share it.
Thanks in advance
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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7, 47 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating
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From: FMonson-at-wcupa.edu
Date: Fri, 10 Mar 2017 09:42:03 -0600
Subject: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Compare FEI 12T and FEI Quanta 400 ESEM, both tungsten emitters (exchangeable).
Quanta 400 ESEM Average life = 60-80 hr
When Quanta vented to change specimen, entire column is vented with AIR (could be better with N2, but not often)
Tecnai 12T (120kV) Average life = 1000 hr**
When change specimen (single tilt) current condition of filament is maintained until vacuum in specimen chamber has recovered.

Quanta filament is OFF overnight.
Tecnai filament is under low current and minimum vacuum pressure 24/7 (10-8 with no break in vacuum in life).

Hope this helps too.

Fred Monson

Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of PA
Geology-Astronomy
750 South Church St.
West Chester, PA, 19383
fmonson-at-wcupa.edu
610-738-0437(Work)


-----Original Message-----
X-from: dcristofori-at-unive.it [mailto:dcristofori-at-unive.it]
Sent: Friday, March 10, 2017 10:18 AM
To: Monson, Frederick {FMonson-at-wcupa.edu}

Dear Listers,
thanks to all of you who answered my question.
LaB6 emitters are unanimously considered stable, yet some of you pointed out that the filament has to be kept heated in order to assure a stable emission, even when not working, e.g. overnight.

I'd like to figure out how common is this approach, and the life of LaB6 emitters operated in this way. It would be very interesting and useful to know your experience also about this point, if you want to share it.
Thanks in advance
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e Dipartimento di Sc. Molecolari e Nanosistemi Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

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18, 44 -- Subject: RE: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving
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From: kraftpiano-at-gmail.com
Date: Fri, 17 Mar 2017 10:27:37 -0500
Subject: [Microscopy] Leo 982 software?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi

LaB6 emitters, like any other source, outgas when first heated and may then
be unstable. A way of getting round this is to run the filament at a very
low current, just to keep it warm, even if the instrument is not being used.
I do not know if they do it now, but JEOL with LaB6 systems set the filament
heating at half value when you "turned it off".
At very low emission there is no source evaporation, so the filament life
does not suffer.

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


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Have not got any messages from the list for a while. Just testing.

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From voraisai2-at-gmail.com Thu Mar 16 13:04:03 2017
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Message-ID: {91004185.C02BA2C8-at-gmail.com}

Does anybody have floppies from an old Leo 982 Gemini column? I just got one in the shop with the hard drive removed, and have to restore the on-board computer. (If you’ve got an old parts Leo 982 and have a spare drive with the software installed, and would be willing to part with it, that would work, too…)

Thank you,

Justin A. Kraft

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From: Gregory.Hendricks-at-umassmed.edu
Date: Sun, 19 Mar 2017 20:28:12 -0500
Subject: [Microscopy] viaWWW:Ultramicrotomes and gridstainers

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Email: pscallio-at-dal.ca Name: Pat Scallion

Organization: Institute for Research in Materials Dalhousie University

Title-Subject: [Filtered] S4700 column hot after a power outage

Message: Hi,
I have seen odd behavior from the Hitachi S4700, after a power outage. When I arrived at work, the
front instrument panel was showing only symbols, not numbers. Also, in addition to other usual
things, the column was very hot, and the metal ticking, like what is heard during a bakeout.

I cannot get any image from the upper, TTL detector, and the beam is showing instability, with
flashes of brightness and image jumping when in use. The Vext is slower to rise as well, and the IP3
starts at 1x10 -8, then rises to 1x10 -7 while the beam is on. The typical vacuum level for IP3 has
been 1x10 -6.

I can send more information if specifics are needed.

Hope someone can help me.

Thanks,
Pat Scallion

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From harremme142-at-gmail.com Sun Mar 19 15:03:37 2017
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Message-ID: {FE6C826B.46F47528-at-gmail.com}

Good Morning Allan,
I just purchased our second Leica UC7. These are great ultramicrotomes; very compact, easy to use and excellent lighting.
I highly recommend them.
Greg

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Email: allan.mitchell-at-otago.ac.nz Name: Allan Mitchell

Organization: Otago centre for Electron Microscopy, University of Otago

Title-Subject: [Filtered] Ultramicrotomes and gridstainers

Message: We are in the process of trying to obtain funding to replace one of our ultramicrotomes (Ultracut E) and our aged LKB Ultrostainer. We are looking at
- RMC Boeckeler ultramicrotme PT-PCZ and the RMC Boeckeler QG-3100 TEM Stainer, or

- Leica Ultracut EM UC7 ultramicrotome and Leica EM AC20 grid-stainer.

I would be keen to hear of peoples experiences with any of these instruments, positives /negatives.

Many thanks

Allan

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From: dcristofori-at-unive.it
Date: Mon, 20 Mar 2017 13:24:24 -0500
Subject: [Microscopy] Re: SEM: are LaB6 emitters fit for EDS and WDS? - moving

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Dear Listers,
here again to sum up the results of my little survey.
It came out that keeping the LaB6 filament heated, even not at a full
range, is quite the standard approach for this emitters.

Many thanks to all the contributors.
Regards

Davide

~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

Centro di Microscopia Elettronica "Giovanni Stevanato" e
Dipartimento di Sc. Molecolari e Nanosistemi
Universit Ca' Foscari Venezia

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~

Il 10/03/2017 16:14, dcristofori-at-unive.it ha scritto:
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} Dear Listers,
} thanks to all of you who answered my question.
} LaB6 emitters are unanimously considered stable, yet some of you pointed
} out that the filament has to be kept heated in order to assure a stable
} emission, even when not working, e.g. overnight.
}
} I'd like to figure out how common is this approach, and the life of LaB6
} emitters operated in this way. It would be very interesting and useful
} to know your experience also about this point, if you want to share it.
} Thanks in advance
} Regards
}
} Davide
}
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
} Davide Cristofori
}
} direct phone = 041.234.67.26
} e-mail = dcristofori[at]unive[dot]it
}
} Centro di Microscopia Elettronica "Giovanni Stevanato" e
} Dipartimento di Sc. Molecolari e Nanosistemi
} Universit Ca' Foscari Venezia
}
} Campus Scientifico, Edificio ETA
} Via Torino 155 I-30172 Mestre (VE) Italy
} ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
}
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} 7, 47 -- From: Davide Cristofori {dcristofori-at-unive.it}
} 7, 47 -- To: microscopy-at-microscopy.com
} 7, 47 -- Subject: SEM: are LaB6 emitters fit for EDS and WDS? - moving to LaB6 heating
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8, 49 -- Subject: Re: [Microscopy] SEM: are LaB6 emitters fit for EDS and WDS? - moving
8, 49 -- to LaB6 heating
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From: microscopy.listserver-at-gmail.com
Date: Tue, 21 Mar 2017 14:03:33 -0500
Subject: [Microscopy] viaWWW: Cost of adding an e-beam lithography to a JEOL SEM

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Email: szou-at-american.edu Name: Shouzhong Zou

Organization: American University

Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM

Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM.
Do you have any ideas of how much this would cost? We just need a basic EBL to make electrical
contact pads for graphene sheets. Thanks.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 23 Mar 2017 17:08:21 -0500
Subject: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane?

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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope Beckman Research Institute

Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the
membrane?

Message: Dear Listers,

We did immunogold labeling on MDA-MB-231 breast cancer cells grown on
cover glass to see if the antigen would appear on the cell surface. The
cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M
cacodylate buffer pH7.4. Immunogold labeling was performed before
critical point drying and sputter-coating with Au/Pd. Images were taken
on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold
particles are localized in one area. An example is here:
https://goo.gl/photos/YPXZrwzNAukfsHBw7
Could you tell what the structure it is? Also, there were a lot of gold
particles on the background/cover glass? Could you suggest ways of
eliminating them.

Thank you in advance!

Zhuo Li

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From: leunissen-at-aurion.nl
Date: Thu, 23 Mar 2017 18:07:31 -0500
Subject: [Microscopy] Re: viaWWW:Immuno-gold labeled cellular structures on the membrane?

Contents Retrieved from Microscopy Listserver Archives
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Good morning,

This is not easy to answer in just a few words.

To evaluate the reliability of any immunolabelling it is required to at least check the negative controls: specimens incubated without primary antibody. Based on those results one can then look at background and what to do about it.
If the negative control is clean, you are dealing with labelling based on binding of the primary. Pre-adsorption of the primary can help provide answers as to whether what you observe is specific or non-specific.
If the negative control is not clean, the labelling is the result of interaction between gold conjugate and specimen. In that case incubation protocols and specimen conditioning are the first thing to look at.

Bakground, false positives can almost always be controlled. I will be happy to help with detailed suggestions if you would like, but since that might go into products and brands it would be better to do this off-list.

Cheers from sunny New Zealand,

Jan Leunissen
Aurion

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From: ZZhang-at-uwyo.edu
Date: Thu, 23 Mar 2017 21:19:10 -0500
Subject: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane?

Contents Retrieved from Microscopy Listserver Archives
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Dear Zhuo Li:

1. It seems, at least, two possibilities for your picture. One is the structure (with gold particles) could be extracellular matrix, which is usually 'sticky' for gold particles, or could contain the antigen. Another possibility is that it is a broken membrane - you are viewing the inner membrane, which might have more antigen, or being sticky. You may want to do a 'control' with full fixation (Glutaraldehyde + Osmium), which provide you a better resolution of the structure.

2. Aldehyde group, especially that from glutaraldehyde is very 'sticky' to antibodies. Since you are NOT viewing the internal structure, I strongly recommend to avoid using glutaraldehyde, not even 0.1%. It may explain, at least in part, the heavy labeling on your cover glass.

3. Blocking with BSA, and/or serum could reduce the non-specific background.

4. I recommend the sample be coated with carbon, instead of Au/Pd. You can then confirm the gold particles with a backscatter electron detector (here is a reference I published many years ago - Localization of myosin on sperm-cell-associated membranes of tobacco (Nicotiana tabacum L.) - https://link.springer.com/article/10.1007/BF01279082

5. As Jan suggested, control is critical!

Hope this helps and let me know if you need a PDF copy of the reference.

Good Luck,

Zhaojie

Zhaojie Zhang, Ph.D.
Director, Jenkins Microscopy Facility
University of Wyoming


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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope Beckman Research Institute

Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?

Message: Dear Listers,

We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some scattered signals, a lot of gold particles are localized in one area. An example is here:
https://goo.gl/photos/YPXZrwzNAukfsHBw7
Could you tell what the structure it is? Also, there were a lot of gold particles on the background/cover glass? Could you suggest ways of eliminating them.

Thank you in advance!

Zhuo Li

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From: l.kepinski-at-int.pan.wroc.pl
Date: Fri, 24 Mar 2017 14:22:50 -0500
Subject: [Microscopy] Open position SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
The Institute of Low Temperature and Structure Research, Polish Academy
of Sciences, Wroclaw, Poland has an immediate opening for an Electron
Microscopy Technician.

The main responsibilities and duties are: operation and maintenance of
FE-SEM (equipped with EDS and EBSD) and ancillary equipment; training
and support for users in microscope operation and sample preparation and
participation in scientific projects in the field of materials science.

Job requirements: Graduate (B.Sc is a minimum) in physics, material
science, chemistry, or similar discipline; experience in operation of
SEM and X-ray microanalysis (EDS) (knowledge of EBSD and TEM techniques
will be an advantage); good written and oral skills in English.

To view full job posting please visit :
http://www.intibs.pl/en/the-institute/news/687

Regards,

Leszek Kepinski

Institute of Low Temperature and Structure Research,

Polish Academy of Sciences,

P.O. Box 1410, 50-950 Wroclaw, Poland

e-mail: L.Kepinski-at-int.pan.wroc.pl

==============================Original Headers==============================
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10, 18 -- From: "l.kepinski" {l.kepinski-at-int.pan.wroc.pl}
10, 18 -- To: Microscopy-at-microscopy.com
10, 18 -- Subject: Open position SEM
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From: xinhuolin-at-gmail.com
Date: Fri, 24 Mar 2017 22:27:48 -0500
Subject: [Microscopy] CryoEM/TEM Postdoc Position at Brookhaven National Laboratory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The postdoctoral associate is expected to develop and perform TEM
characterization of assembly of designed bio-molecular arrays. The
research will involve the development of new approaches for cryoEM,
cryoET, cryoSTEM, liquidEM characterization of an integration of
proteins, peptides and DNA into targeted structures with multiscale
organization, and analysis of their structure and assembly processes.
The work will be conducted in the Electron Microscopy group in the
Center for Functional Nanomaterials which houses five state-of-the-art
scanning/ transmission electron microscopes. The majority of the work
will be conducted on a FEI Environmental Titan equipped with a K2
direct-electron detector, a FEI Talos F200X, and an
aberration-corrected dedicated STEM, first two of which have
cryo-transfer, liquid-flow, low-dose, and automated data acquisition
capabilities. The successful implementation of the work requires a
close interaction with scientists working on bio-inspired
self-assembly and advanced characterization methods using x-ray
scattering.

Review of applications begins immediately. Applications will be
accepted until the position is filled. Research will be under the
direction of two well-known electron microscopists at the CFN in close
collaboration with PIs and postdocs from the Soft/Bio Group, the
Biology Department, and NSLSII.

Required Knowledge, Skills and Abilities:

1. Ph.D. in Physics, Materials Science, Chemistry or Biology
2. Solid background in Transmission Electron Microscopy with rigorous
Ph.D. training in a cryoEM or TEM group.
3. Skills in cryoEM/TEM structural characterization at the atomic scale.

Preferred Knowledge, Skills, and Abilities:

Cryo-electron microscopy, single-particle and tomography methods,
hands-on experience with TEM sample preparation, such as cryo-plunging
and ultramicrotomy

Other Information:

BNL policy states that Research Associate appointments may be made to
those who have received their doctoral degrees within the past five
years.

The EM facility in the CFN includes 5 transmission electron
microscopes, 1 dual-beam FIB, and a collection of specialized holders
including liquid and gas flow holders, liquid electrochemical holders,
heating holders (single-tilt, double-tilt, and high tilt),
cryo-transfer holders (single tilt, double tilt, and high tilt), and
nanoindentation holders (single and double tilt).

Interested candidates can apply online by clicking on the link below:

https://jobs.bnl.gov/job/upton/postdoctoral-research-associate-cryo-em-tem/3437/4240079


Best Regards,
Huolin Xin, Ph.D.
Associate Scientist
Center for Functional Nanomaterials at Brookhaven National Laboratory
and
Adjunct Assistant Professor
Department of Materials Science and Engineering
SUNY Stony Brook University
https://sites.google.com/site/xinhuolin/
Email: hxin-at-bnl.gov
Office: 631-344-4350

==============================Original Headers==============================
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13, 37 -- Subject: CryoEM/TEM Postdoc Position at Brookhaven National Laboratory
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From: xinhuolin-at-gmail.com
Date: Fri, 24 Mar 2017 22:32:21 -0500
Subject: [Microscopy] STEM/Tomography Postdoc Position at Brookhaven National Laboratory

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear TEMers,

I am looking for a postdoc in the area of STEM and electron
tomography. The postdoc will have the opportunity to work with a suite
of tools in the Center for Functional Nanomaterials at Brookhaven
National Laboratory, including an aberration-corrected FEI
Environmental Titan equipped with a K2 direct-electron detector, a FEI
Talos F200X equipped with X-FEG, Super-X, and an Enfinium, an
aberration-corrected cold-FEG dedicated STEM, and a Nion HERMES
(located in Rutgers), first two of which have analytical tomography,
cryoET, cryoEM, and liquidEM capabilities. In total, the facility
includes 5 transmission electron microscopes, 1 dual-beam FIB, and a
collection of specialized holders including liquid and gas flow
holders, liquid electrochemical holders, heating holders (single,
double, and high tilt), cryogenic holders (single tilt, double tilt,
and high tilt), and nanoindentation holders (single and double tilt).

Interested applicants should send a CV, and a one-paragraph
description of his/her research/education background. Review of
applications begins immediately. Applications will be accepted until
the position is filled.

Best Regards,
Huolin Xin, Ph.D.
Associate Scientist
Center for Functional Nanomaterials at Brookhaven National Laboratory
and
Adjunct Assistant Professor
Department of Materials Science and Engineering
SUNY Stony Brook University
https://sites.google.com/site/xinhuolin/
Email: xinhuolin-at-gmail.com
Office: 631-344-4350

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Mar 2017 11:44:26 -0500
Subject: [Microscopy] viaWWW:Immuno-gold labeled cellular structures on the membrane?

Contents Retrieved from Microscopy Listserver Archives
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This Question/Comment was submitted to the Microscopy Listserver
using the WWW based Form at http://www.microscopy.com/MLFormMail.html
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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope Beckman Research Institute

Title-Subject: [Filtered] Immuno-gold labeled cellular structures on the membrane?

Message: Dear Listers,

We did immunogold labeling on MDA-MB-231 breast cancer cells grown on cover glass to see if the
antigen would appear on the cell surface. The cells were fixed in 4% paraformaldehyde and 0.1%
glutaraldehyde in 0.1M cacodylate buffer pH7.4. Immunogold labeling was performed before critical
point drying and sputter-coating with Au/Pd. Images were taken on a Zeiss Sigma FE-SEM. Besides some
scattered signals, a lot of gold particles are localized in one area. An example is here:
https://goo.gl/photos/YPXZrwzNAukfsHBw7
Could you tell what the structure it is? Also, there were a lot of gold particles on the
background/cover glass? Could you suggest ways of eliminating them.

Thank you in advance!

Zhuo Li

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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Mar 2017 11:45:11 -0500
Subject: [Microscopy] viaWWW: The Current Level in ET Detectors After the PRE-AMP stage

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Email: anirudha-at-sciencetomorrow.biz Name: Anirudha Borse

Organization: ScienceTomorrow LLC

Title-Subject: [Filtered] The Current Level in ET Detectors After the PRE-AMP stage

Message: Hi,

I have recently started studying SEMs and was looking into the Detector Current value after the
Pre-Amplifier stage and before converting it to Digital Voltage.

Can anyone please help me with that

Thank You
Anirudha
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Mar 2017 21:51:16 -0500
Subject: [Microscopy] viaWWW:EM Technical Director position at University of Chicago

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Email: jotham-at-uchicago.edu Name: Joe Austin

Organization: The University of Chicago

Title-Subject: [Filtered] EM Technical Director position at University of Chicago

Message: Dear Colleagues,

I would like to call your attention to a Technical Director position in the Advanced Electron
Microscopy facility at The University of Chicago (Requisition Number 102322).

For more information about the position and to apply please visit this link:
https://jobopportunities.uchicago.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=670183

This position will be open until filled and will be reviewing applications on an ongoing basis.
If you have questions about the position please email Joe Austin at jotham-at-uchicago.edu.
If you have any difficulty uploading your application or any questions, please email Manuel
Carrasquillo at mcarrasquillo-at-bsd.uchicago.edu


The University of Chicago is an Affirmative Action/Equal Opportunity/Disabled/Veterans Employer and
does not discriminate on the basis of race, color, religion, sex, sexual orientation, gender
identity, national or ethnic origin, age, status as an individual with a disability, protected
veteran status, genetic information, or other protected classes under the law. For additional
information please see the University's Notice of Nondiscrimination.
Staff Job seekers in need of a reasonable accommodation to complete the application process should
call 773-834-1841 or email talentacquisition-at-uchicago.edu with their request.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 28 Mar 2017 19:01:35 -0500
Subject: [Microscopy] viaWWW:Cathodoluminescence in SEM

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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] Cathodoluminescence in SEM

Message: We are in the process of adding CL to our FE-SEM. I am not familiar enough with the
detectors to know if this is a pain or a positive.

Some questions would be: What is the average lifespan (if there is one) for these detectors? What
kind of trouble can you get into with them? Are there any conflicts with other detectors (real or
imagined)? How easy is it to use one?
Any information that cannot be easily googled would be appreciated.

John S

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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Mar 2017 07:56:07 -0500
Subject: [Microscopy] viaWWW:multiple grid cryo holder

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Email: j.janssen-at-nki.nl Name: Hans Janssen

Organization: The Netherlands Cancer Institute

Title-Subject: [Filtered] multiple grid cryo holder

Message: Does anybody out there have any experience with the Gatan multiple(3)grid cryo holder? We
are specifically interested in the grid locking system.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Apr 2017 06:59:20 -0500
Subject: [Microscopy] viaWWW:Haskris Chiller R075

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Email: Hasan.Ali-at-angstrom.uu.se Name: Hasan Ali

Organization: Uppsala University

Title-Subject: [Filtered] Free Lens Control On JEOL2000FX

Message: Hi,

We have assembled a Gatan energy filter GIF2002 under an old JEOL2000FX. As the GIF doesn't have any
communication with the microscope, we don't have a magnification reduction in the microscope when
using the GIF and the image/diffraction pattern on the viewing screen is magnified about 19X on the
GIF CCD. I want to do angular resolved EELS, but even at the lowest automated camera length (100mm)
in the microscope, I cannot get the two beams simultaneously on the CCD for my sample (the
collection angle is too low). So I need to go to lower camera lengths using "Free Lens Control"
available in the microscpe. I tried Free lens control and obtained the lower camera lengths by
changing the strength of intermediate lenses, but when I switch back to image mode, the settings
there are changes too and the sample is no more on eucentric height. I don't understand exactly now
which lens should I tune in combination with intermediate lenses.
Does anyone have the experience of operating the microscope in Free lens control who could suggest
me something to follow? Also if someone have the manual to operate the JEOL2000FX in Free Lens
Control, that will be very helpful for me. I didn't get information about this in the manuals which
I have.
Thanks

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From fritschemichael755-at-gmail.com Fri Mar 31 04:20:40 2017
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Email: kmoore-at-vetcor.com Name: Kasandra Moore

Organization: Geist Station Animal Hospital

Title-Subject: [Filtered] Maintenance Microscope
Message: We have a Swift Instrument international SA M3200 microscope.
The images are dirty.
We think the microscope needs to be cleaned.
Can someone provide this service in the Indianapolis area?

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From westelle638-at-gmail.com Sun Apr 2 14:48:15 2017
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Message-ID: {AB55A17E.C16BAE9B-at-gmail.com}

The 53rd anniversary meeting of the Southeastern Microscopy Society will be at the Holiday Inn in Athens, GA, on May 24-26, 2017! There is an exciting program planned; Mike Marko of the Wadsworth Center and past president of MSA will be our Invited Speaker. In addition, there will be contributed talks from the members and the Ruska Award student presentations. (Encourage your students to compete in the Ruska Award competition!) A Vendor Social will be held on Wednesday evening, a Banquet on Thursday evening, and a Business Breakfast on Friday morning.

Information is available at http://southeasternmicroscopy.org/2017-2/

Half-day Workshops on Wednesday will include:

RMC and an Array ultramicrotome (at Holiday Inn)
FEI and FE-SEM STEM and analysis (at GEM on UGA campus)
Negative staining with Sara Miller and Mary Ard (at GEM on UGA campus)
Protochips with wet/dry TEM sample holder. (at GEM on UGA campus)
Possible Confocal workshop with Zeiss (at the BioImaging Center at UGA)

The abstract deadline is April 14. Please encourage your students to apply for the Ruska Award! http://southeasternmicroscopy.org/ruska-award-student-competition/

The SEMS website is ready for your registration, available at http://southeasternmicroscopy.org/2017-2/ . You can also get to the hotel reservation webpage by clicking on the link on the meeting website.

We hope to see you in Athens!

Terri Bruce (terri-at-clemson.edu), Program Chair
John Shields (johnshields-at-gmail.com) and Mary Ard (maryard-at-uga.edu), Local Arrangements Committee



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Email: andre.dufresne-at-umanitoba.ca Name: Andr Dufresne

Organization: University of Manitoba Canada

Title-Subject: [Filtered] Haskris Chiller R075

Message: Hy everyone,
I'm looking for a water chiller for a TEM and SEM (H-7000)
The unit I'm looking for is a Haskris R075 or equivalent.
Options:
Water cooled

Message me directly if you have any questions or know the availability of such a unit.
Merci /Thank you.

AD

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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Apr 2017 07:00:16 -0500
Subject: [Microscopy] viaWWW:Aurion Immuno Gold Silver Staining Workshop

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] The Aurion Immuno Gold Silver Staining Workshop

Message: Three days of hands-on training for students, researchers, and microscopists who want to
learn the most up to date theory and practice in Immuno Gold labeling.
Details:
Wednesday - Friday
May 9-12, 2017
8:00 a.m. - 4:30 p.m.
EMS Microscopy Academy
Hatfield, Pennsylvania, USA

Instructors:
Peter Van De Plas, Aurion, The Netherlands
Michael Kostrna, Director, EMS Microscopy Academy
Al Coritz, Technical Director, Electron Microscopy Sciences

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From: zaluzec-at-aaem.amc.anl.gov
Date: Tue, 11 Apr 2017 07:15:49 -0500
Subject: [Microscopy] In Memoriam: B. Kestel MSA 1994 Technologist of the Year

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Email: gtorraca-at-amgen.com Name: Gianni Torraca

Organization: MSA

Title-Subject: [Filtered] Freeze fracture TEM of biologics wanted - service work

Message: Good Day,

I am looking for a lab that can perform freeze fracture analysis of a biologic solution.
Kinds regards
Gianni Torraca
Sr. Scientist
Amgen Inc.

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From kimgarr459-at-gmail.com Fri Apr 7 13:02:06 2017
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Message-ID: {A014D037.891BAFC7-at-gmail.com}

Bernard J. "Bernie” Kestel the 1994 Microscopy Society of America’s Technologist of the year passed away
on April 7.

Bernie was an engineering specialist microscopy technologist for 44 years at Argonne National Laboratory, retiring in 2002. Bernie published numerous articles in microscopy journals and was the recipient of the 1994 Technologist of the Year by the Microscopy Society of America, 1996 Pacesetter Award by Argonne National Laboratory and 1998 Outstanding Service Award by the University of Chicago.

His contributions to the community were in the area of specimen preparation, where he developed new techniques, preparated countless specimens and assisted/trained innumerable students, scientists and colleagues. A compendium of his methodology has been published as a scientific report and is freely available to anyone interested in electropolishing of materials.

Kestel B, (1986) Polishing Methods for Metallic and Ceramic Transmission Electron Microscopy Specimens, ANL-80-120/Rev.1 Report , available from NTIS DE89016686 Issue Number 199005 https://ntrl.ntis.gov/NTRL/dashboard/searchResults.xhtml?searchQuery=ANL-80-120

He was an artist in his field and could always be counted on to find a way to make that critical TEM sample, even when you only had 1small item to work with. He will be remembered here at Argonne and by those he helped in the community, myself included.


http://www.friedrichjones.com/obits/obituaries.php/obitID/985197?utm_source=Argonne+Today&utm_campaign=fd54afd940-ATS_2017_04_11&utm_medium=email&utm_term=0_91cdd2aa04-fd54afd940-237434973

===========================================
Dr. Nestor J. Zaluzec
Argonne National Laboratory
Photon Sciences Division
9700 S. Cass Ave
Bldg 212 / A-143
Argonne, Illinois 60439 USA
Email: Zaluzec-at-aaem.amc.anl.gov


Tel: 530-NES-TORZ (530-637-8679) has Voice Mail
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Senior Scientist - Argonne National Laboratory
Fellow of the Microscopy Society of America
Senior Fellow the Computational Institute - University of Chicago
E.P. Wigner Fellow - Oak Ridge National Laboratory
Past President Microscopy Society of America
Adjunct Professor of Physics - Northern Illinois University &
the University of Illinois at Chicago
Visiting Professor of Microscopy - Manchester University

===========================================
TPMLab: http://tpm.amc.anl.gov
MMSite: http://www.amc.anl.gov
===========================================

The box said ...
"This program requires Win 95/98/NT or better..."
So I bought a Mac !

===========================================
LLAP
=========================================+=====



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From: cannonmp-at-comcast.net
Date: Thu, 13 Apr 2017 02:19:50 -0500
Subject: [Microscopy] Pinouts for Oxford Pentafet Pre-Amp

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
**any reply should go directly to the poster**
as well as to the list.
Using the "reply" function in your email does *not* send your answer
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***********************************************************************************
Name: Richard Gursky
School: St. Jude Children’s Research Hospital
Grade/Education Level: Graduate
Location: Memphis, TN
US
Email: richard.gursky-at-stjude.org

***********************************************************************************
Forwarded from "Ask a Microscopist"
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***********************************************************************************
Listers,

This is mostly for those of you on the list who work as applications specialists or service engineers for microscope companies. Perhaps you can send some good advice to Brakel about how to get such a job.
Please remember to respond directly to Brakel -- kbrakel91-at-gmail.com

I've already sent some ideas to the Brakel, but since I do not work as an applications person or a service engineer, some pointers from people who do would be more useful.

---------------
Philip Oshel
Microscopy Society of America
Ask a Microscopist
www(dot)microscopy(dot)org/resources/ask(dot)cfm

Name: Kiralyn Brakel
School: Texas A&M University
Grade/Education Level: Graduate
Location: College Station, TX
US
Email: kbrakel91-at-gmail.com

I'm trying to find the pre-amp pinout designations on an Oxford 7215 Link
Pentafet EDS spectrometer. Oxford no longer supports this once highly
touted piece of gear and have been unable to help me. Can anyone help with
that?

Keeping legacy gear running is made much more difficult by lack of support.
I have operated a small electron microprobe lab here in Seattle since 1984
and have useful gear from the entire history of x-ray microanalysis. I'm
now using an old ARL SEMQ and a JEOL JXA 8600. I once had the ARL EMX-SM
that had the actual moon rocks in it. My computers go all the way back to
DEC PDP11s, though that's long gone. The SEMQ still uses MS 6.2. I have
thought it might be good to have a used gear forum of some sort. Facebook
page ? Wordpress blog ? Any thoughts on that too.

Bart Cannon / Cannon Microprobe


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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Apr 2017 06:38:42 -0500
Subject: [Microscopy] viaWWW:celsian or barium feldspar specimens

Contents Retrieved from Microscopy Listserver Archives
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Email: Linda.Davis-at-Vesuvius.com Name: Linda L Davis

Organization: Vesuvius USA

Title-Subject: [Filtered] celsian or barium feldspar specimens

Message: Hello All,

We have a new chemist in our R&D Analytical Group who needs to do some development work on analyzing
barite and celsian using an ICP. I've found nice barite crystals to purchase, but now I need some
celsian/barium feldspar. I cannot use eBay at all for work, so I have come to the listserve to see
if anyone has any celsian we can purchase or have. I don't need a ton, but numerous crystals/grains
to grind up for digestion and analysis.
I would greatly appreciate any help.

Sincerely,

Linda L. Davis

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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Apr 2017 06:39:32 -0500
Subject: [Microscopy] viaWWW: Whole Cell Mount TEM - Carbon Coating

Contents Retrieved from Microscopy Listserver Archives
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Email: vakimler-at-oakland.edu Name: Vickie Kimler

Organization: Oakland University Eye Research Institute

Title-Subject: [Filtered] Whole Cell Mount TEM - Carbon Coating

Message: When one does whole cell mount TEM nowadays on gold-Formvar grids, is it necessary to
carbon-coat the samples after preparation (seeding, fixation, staining, dehydration and critical
point drying by CO2) or is before the preparation sufficient?

Grids with cells will be stored in a dessicator filled with Drier-Rite.

Thanks,
Vickie

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From: microscopy.listserver-at-gmail.com
Date: Sun, 16 Apr 2017 08:58:39 -0500
Subject: [Microscopy] viaWWW:Electron Microscopy Technologist Position Open

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Name: Richard Gursky
School: St. Jude Children’s Research Hospital
Grade/Education Level: Graduate
Location: Memphis, TN
US
Email: richard.gursky-at-stjude.org

Hello Shouzhong,

I would contact Joe Nabity to see if his Nanometer Pattern Generation
System can be added to your 'scope. We use it on our FIB and are happy
with the performance. You can find more information here:
http://www.jcnabity.com/

I have no ties, financial or otherwise, to Joe or his company.

Thanks,
Chris

On Tue, Mar 21, 2017 at 3:15 PM, {microscopy.listserver-at-gmail.com} wrote:
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} Email: szou-at-american.edu Name: Shouzhong Zou
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} Title-Subject: [Filtered] Cost of adding an e-beam lithography to a JEOL SEM
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} Message: Hi Everyone. We are interested in adding an e-beam lithography to a JEOL JSM-IT100LA SEM.
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Email: mryan-at-cshl.edu Name: Marjorie Ryan

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Searching for an Electron Microscopy Technologist

Message: Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Apr 2017 21:18:48 -0500
Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS

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On Apr 14, 2017, at 7:39 AM, microscopy.listserver-at-gmail.com
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} Title-Subject: [Filtered] celsian or barium feldspar specimens
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} Message: Hello All,
}
} We have a new chemist in our R&D Analytical Group who needs to do some development work on analyzing
} barite and celsian using an ICP. I've found nice barite crystals to purchase, but now I need some
} celsian/barium feldspar. I cannot use eBay at all for work, so I have come to the listserve to see
} if anyone has any celsian we can purchase or have. I don't need a ton, but numerous crystals/grains
} to grind up for digestion and analysis.
} I would greatly appreciate any help.
}
} Sincerely,
}
} Linda L. Davis
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} 15, 53 -- Subject: viaWWW:celsian or barium feldspar specimens
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From wandjoan59-at-gmail.com Mon Apr 17 04:05:20 2017
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Email: ravi.thakkar369-at-gmail.com Name: Ravi

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Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.

Message: Hi All,
One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference
for EDS analysis. If any one can help. Thanks in advance.
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12, 54 -- Subject: viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS
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From: wesaia-at-iastate.edu
Date: Tue, 18 Apr 2017 05:55:43 -0500
Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS

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We may need more information before we can answer the question well - at least, I would need more.

Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two applications.
- If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or powder.
- If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might be challenging to find it in bulk form.

What do they ultimately want to know? Do they want to know if their material matches? Do they want to quantify the oxide? Do they want to see if they have excess oxygen?
I often find users coming in with too narrow a question. When I found out the true issue, there is usually much more freedom in suggesting a solution.

Why do they want the oxides? Most EDS systems will have standards built-in for the elements. They are often quite good. I wouldn't think that they need the oxides of the metals.

What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material, then accurate quant will be out of the question.

Hopefully the answer is not simply "Because". I find it much easier to help someone who is forthcoming with information rather than one who is dead-set on a single course of action.

Regards,
Warren Straszheim

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Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.

Message: Hi All,
One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard reference for EDS analysis. If any one can help. Thanks in advance.
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25, 57 -- Subject: RE: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide
25, 57 -- sample for EDS
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From: kleopullin-at-email.arizona.edu
Date: Tue, 18 Apr 2017 18:31:44 -0500
Subject: [Microscopy] HRTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'm struggling with Williams and Carter (2nd edition, I think)
understanding HRTEM. I generally find their text approachable and easy
to read, but not the HRTEM material, other than the math.

Is their a text or article that is more detailed? Outside of the
Supposedly HRTEM sources that I have found have lengthy introductions
to the basic, non-HR microscope, then brief descriptions of the math
of HRTEM.

What's a good Read?

I'm a microscopist, with a B.S., so technical is okay, but I want a
deep focus on HRTEM, theory and instrumentation.

Thanks!

Kleo (Kathleen) Pullin
Moraga, CA
209-610-0555
kleopullin-at-email.arizona.edu
https://www.linkedin.com/in/kleopullin
https://twitter.com/resolvingdust

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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Apr 2017 19:31:21 -0500
Subject: [Microscopy] Re: viaWWW:Source of Standard zinc oxide and iron oxide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Ravi,

There are some sources for standards mentioned in this thread:

http://probesoftware.com/smf/index.php?topic=889.msg5674#msg5674

john


On 4/17/2017 7:28 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.
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--
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10, 51 -- Subject: Re: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide
10, 51 -- sample for EDS
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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Apr 2017 19:31:51 -0500
Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron

Contents Retrieved from Microscopy Listserver Archives
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I am not affiliated with Ted Pella, but they do sell EDS/WDS mineral/oxide standards.

http://www.tedpella.com/calibration_html/UHV-EL_Reference_Standards_for_EDS_WDS.htm



On Tue, Apr 18, 2017 at 7:17 AM, {wesaia-at-iastate.edu {mailto:wesaia-at-iastate.edu} } wrote:




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We may need more information before we can answer the question well - at least, I would need more.

Is this EDS in conjunction with TEM or SEM? The form of the material would different for the two
applications.
- If TEM, I don't have much to suggest since I do SEM. It seems they would want a thin film or
powder.
- If SEM, they would probably want a homogenous, bulk sample. They can get bulk samples of iron
oxide. Does it matter if it is Fe2O3 or Fe3O4? I am used to zinc oxide being a powder. It might
be challenging to find it in bulk form.

What do they ultimately want to know? Do they want to know if their material matches? Do they
want to quantify the oxide? Do they want to see if they have excess oxygen?
I often find users coming in with too narrow a question. When I found out the true issue, there
is usually much more freedom in suggesting a solution.

Why do they want the oxides? Most EDS systems will have standards built-in for the elements.
They are often quite good. I wouldn't think that they need the oxides of the metals.

What form is their sample in, bulk, powder, film? If it is not flat, polished, thick material,
then accurate quant will be out of the question.

Hopefully the answer is not simply "Because". I find it much easier to help someone who is
forthcoming with information rather than one who is dead-set on a single course of action.

Regards,
Warren Straszheim

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Subject: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide sample for EDS




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Organization: Ravi Thakkar

Title-Subject: [Filtered] Source of Standard zinc oxide and iron oxide sample for EDS reference.

Message: Hi All,
One of the user for our EM facility is looking for Zinc Oxide and Iron Oxide as standard
reference for EDS analysis. If any one can help. Thanks in advance.
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25, 57 -- From: "Straszheim, Warren E [BIOTC]" {wesaia-at-iastate.edu {mailto:wesaia-at-iastate.edu} }
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25, 57 -- Subject: RE: [Microscopy] viaWWW:Source of Standard zinc oxide and iron oxide
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From: microscopy.listserver-at-gmail.com
Date: Tue, 18 Apr 2017 19:46:09 -0500
Subject: [Microscopy] viaWWW:need 700-800 nm excitation for Zeiss AxioObserver

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Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer

Organization: NYULMC

Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver

Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered
to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are
looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber
that we could swap for the Zeiss HPX-120 on the days we need to image infra-red.

Are there any simple light sources that fit this description that cost less than $3k? If not, how
about ones that don’t cost much more than this?

Thank you!


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From: nmz787-at-gmail.com
Date: Tue, 18 Apr 2017 20:51:45 -0500
Subject: [Microscopy] Re: viaWWW:need 700-800 nm excitation for Zeiss AxioObserver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I'd say it depends on how stable you want the line (frequency) to be.

CD-ROM lasers are 780nm, and super cheap thanks to economies of scale.
Getting one coupled to the fiber of your choice would then be your
challenge.

On Tue, Apr 18, 2017 at 6:08 PM, {microscopy.listserver-at-gmail.com} wrote:
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} Email: michael.cammer-at-med.nyu.edu Name: Michael Cammer
}
} Organization: NYULMC
}
} Title-Subject: [Filtered] need 700-800 nm excitation for Zeiss AxioObserver
}
} Message: We need to excite in the 700 to 800 nm range and the Zeiss HPX-120 lamp we have is filtered
} to block wavelengths above 660 nm. Rather than customize the filter inside the lamp house, we are
} looking for an alternative light source for the 700-800 nm range with a liquid light guide or fiber
} that we could swap for the Zeiss HPX-120 on the days we need to image infra-red.
}
} Are there any simple light sources that fit this description that cost less than $3k? If not, how
} about ones that don’t cost much more than this?
}
} Thank you!


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From: benada-at-biomed.cas.cz
Date: Wed, 19 Apr 2017 09:52:13 -0500
Subject: [Microscopy] Re: FW: Ask a Microscopist- converting EDS files in

Contents Retrieved from Microscopy Listserver Archives
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***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
**any reply should go directly to the poster**
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-----Original Message-----
X-from: "AssociationManagement-at-microscopy.org" {associationmanagement-at-microscopy.org}

Hello Anuja,
Please look at the WEB page of NIST DTSA II software.

{http://www.cstl.nist.gov/div837/837.02/epq/dtsa2/}

It should be possible to import .spx file into this software and then export the data in cvs format.
Unfortunately I do not have any spx file at hand, but I have tested it on msa and spc files and it works well.

Best regards from Prague

Oldrich

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Wed, 19 Apr 2017 06:59:11 -0500, oshel1pe-at-cmich.edu wrote :
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} -----Original Message-----
} X-from: "AssociationManagement-at-microscopy.org"
} {associationmanagement-at-microscopy.org} Date: Wednesday, 19April,
} 2017 at 01:08 To: "AssociationManagement-at-microscopy.org"
} {associationmanagement-at-microscopy.org} , Philip Oshel
} {oshel1pe-at-cmich.edu} Subject: Ask a Microscopist
}
} Name:Anuja Bhalkikar
} School:University of Nebraska Lincoln
} Grade/Education Level:Graduate
} Location:Lincoln, NE
} Email:anuja.bhalkikar-at-huskers.unl.edu
} Subject:Convert .spx data to .txt or .csvYour
} Question:Hello, I recently used an FEI Tecnai Osiris S/TEM to obtain
} EDS of my sample. I believe we have a Super-X EDX detection system in
} conjunction with Bruker Esprit software. The data was unfortunately
} saved as .spx. Is there any way I could convert it into .txt or .csv
} format? Thanks, Anuja
}
}
}
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From: jkrupp-at-deltacollege.edu
Date: Wed, 19 Apr 2017 15:29:05 -0500
Subject: [Microscopy] Update on Spurr's Plastic Please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings

I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.

I have used it in the past, it was OK, and never worried about it.

Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.

I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: peter.eschbach-at-comcast.net
Date: Thu, 20 Apr 2017 00:02:50 -0500
Subject: [Microscopy] Re: HRTEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Kleo

Check out the Oxford text: high Resolution Electron Microscopy by Spence.

A close second and less Graduate level math is found in "Transmission Electron Microscopy and Diffractory Materials" Springer by Fultz and Howe.

You are looking for a discussion of "Pendellosung". Page 631 Fultz and Howe. To explain it to Students at U of O that I taught, I built a coupled pendulum, where the one pendulum represents the non diffracted beam and the other s Bragg reflected beam. The coupling represents the lattice. The pendulum will stop and start and that time can represent thickness of da sample. And so white spots in HRTEM , nodes, could be channels between atoms or atoms depending on how thick the sample is ( how much time pendulum swings )


Pete Eschbach
Oregon State University

Sent from my iPhone

} On Apr 18, 2017, at 4:47 PM, kleopullin-at-email.arizona.edu wrote:
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} I'm struggling with Williams and Carter (2nd edition, I think)
} understanding HRTEM. I generally find their text approachable and easy
} to read, but not the HRTEM material, other than the math.
}
} Is their a text or article that is more detailed? Outside of the
} Supposedly HRTEM sources that I have found have lengthy introductions
} to the basic, non-HR microscope, then brief descriptions of the math
} of HRTEM.
}
} What's a good Read?
}
} I'm a microscopist, with a B.S., so technical is okay, but I want a
} deep focus on HRTEM, theory and instrumentation.
}
} Thanks!
}
} Kleo (Kathleen) Pullin
} Moraga, CA
} 209-610-0555
} kleopullin-at-email.arizona.edu
} https://www.linkedin.com/in/kleopullin
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From: Joseph.Mowery-at-ARS.USDA.GOV
Date: Thu, 20 Apr 2017 09:41:54 -0500
Subject: [Microscopy] Update on Spurr's Plastic Please

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning,

After trying Epon, I’ve never felt the need to use Spurr's again. For some reason the Spurr’s recipe proportions vary between some vendors. I’ve been really happy with the LX-112 (Epon) from LaddResearch, their mixing instruction are right on point, and I can store the 2 components in syringes in the fridge for about 6 months and just mix them with the accelerator prior to use. Epon seem to section better than Spurr's, and is more stable under the beam. I've also heard good things about the Epon-Araldite mixture.

-Joe

Joe Mowery | Biologist
Electron and Confocal Microscopy Unit
USDA, Agricultural Research Service
Beltsville, MD



-----Original Message-----
X-from: jkrupp-at-deltacollege.edu [mailto:jkrupp-at-deltacollege.edu]
Sent: Wednesday, April 19, 2017 4:49 PM
To: Mowery, Joseph

Greetings

I’m trying to round out my understanding of different embedding media and need the latest opinions on Spurr’s LVEM.

I have used it in the past, it was OK, and never worried about it.

Now I see that some workers avoid it due to more toxic ingredients, more difficulty staining, and problems with brittle blocks. Also, like Epon 812 before, I see that some of the original components have been replaced with newer substitutes.

I teach students how to embed tissue and want to be able to tell them the latest opinions from other experts in case I am missing something.

Thanks

Jon

Jonathan Krupp
ASB&T
San Joaquin Delta College
5151Pacific Ave.
Stockton, CA 95207
(209) 954-5284
jkrupp-at-deltacollege.edu



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From: bozhilov-at-ucr.edu
Date: Tue, 25 Apr 2017 13:03:18 -0500
Subject: [Microscopy] XL30-FEG SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
We are interested in anyone having an old Gatan GIF 200, or a more recent EEL spectrometer for a Tecnai 30, who needs to have it taken off their hands.
Thanks,

Ken

Johns Hopkins University

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From watkinsmelanie58-at-gmail.com Mon Apr 24 19:52:57 2017
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Here at UC Riverside we have a Philips XL30-FEG SEM that we are planning to remove from service.
If anyone is interested in acquiring this system please contact us.

The SEM is in perfect fully operational condition.
It was installed in 1996 and it has been under continuous parts and labor maintenance contract with the FEI Co. since.
Here i