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From: microscopy.listserver-at-gmail.com
Date: Mon, 1 Jan 2018 08:55:50 -0600
Subject: [Microscopy] Administrivia: Happy New Year 2018

Contents Retrieved from Microscopy Listserver Archives
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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2017, the ListServer delivered your messages to more than 4350 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated ~ 250+ Gbits of Email traffic and ~ 2.55 Million
Email messages were sent out this year by my tired and old little server. Up abit
from last year, but still a steady flow.


As usual you don't want to know how much Junk Mail and spam has been filtered out
far more than you might expect. Apologies to those that have problems with
my filters.


The complete Microscopy ListServer Archives for 2017-1994 (~ are on-line at

http://www.microscopy.com.

A couple of IMPORTANT reminders:

If you leave on vacation/holiday use the on-line form to UNSUBSCRIBE
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Do not reply to message with the return address of:

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If you see a message that has this "No-Reply" return address please post your
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As always if you have questions about suitability of postings or
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Cheers,

Nestor
Your Friendly Neighborhood SysOp


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From: microscopy.listserver-at-gmail.com
Date: Sat, 6 Jan 2018 06:12:28 -0600
Subject: [Microscopy] viaWWW:Applications Scientist In Situ TEM - immedate opening at

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jpshield-at-uga.edu

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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop

Message: This intensive, three-day workshop will provide a practical and basic theoretical
introduction to the Transmission Electron Microscope and biological sample preparation techniques.
Each day will consist of lecture, discussion and hands-on training led by GEM staff.
Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday through Wednesday, March 14-16, 2018, 8am-5pm each day (lunch is provided)

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login

Deadline: March 5, 2018
*****************
Past participants include Merck & Co., Connecticut State, Breathitt Vet Center, Univ. of Chicago,
Stowers Inst. for Medical Research, West Virginia State


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From wandjoan59-at-gmail.com Sat Jan 6 01:56:15 2018
Return-Path: {wandjoan59-at-gmail.com}
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Email: ben-at-protochips.com Name: Benjamin Jacobs

Organization: Protochips, Inc

Title-Subject: [Filtered] Applications Scientist In Situ TEM - immedate opening at Protochips

Message: Protochips has an immediate opening for a marketing applications scientist. Candidates must
have a strong background in TEM, and experience with in situ TEM such as heating, gas, liquid or
mechanical, is highly preferred.
Travel up to 40%, mostly domestic, but some international to Europe and East Asia.
Candidates must also be a US citizen or already be authorized to work in the US.
Position is located in the Raleigh/Durham area in North Carolina, and candidate must be willing to
relocate.

Please click the following link for more information:

http://www.protochips.com/news/marketing-applications-scientist/

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Jan 2018 17:54:21 -0600
Subject: [Microscopy] viaWWW:Lehigh University Microscopy School 2018

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Email: walbrid-at-cshl.edu Name: Samantha Walbridge

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Hiring - Research Associate - Electron Microscopy Technologist Position

Message:
Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
state-of-the-art Microscopy Shared Resource.

The individual must have extensive practical expertise in biological sample preparation for
transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on
knowledge of confocal and widefield fluorescence microscopy would also be a plus.

The candidate will help users design innovative experiments and they will carry out sample
preparation and imaging as well as assist in data interpretation.

Excellent verbal and written communication skills, ability to work with multiple users in a
supporting role, and ability to work independently and proactively with limited supervision are
essential. A Bachelor’s degree in biology or related discipline is required. One to three years of
experience working in a Microscopy Shared Resource is preferred.

How to Apply

Interested individuals should apply for this position via the CSHL careers website at
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Position Number 01779-R

Applicants should include a resume along with a description of their practical expertise and the
names as well as email addresses of 3 references.

Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized
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Email: SLC6-at-Lehigh.edu Name: Sharon Coe

Organization: Lehigh University

Title-Subject: [Filtered] Lehigh University Microscopy School 2018

Message: Now accepting registrations for the 48th Lehigh Microscopy School which will be held on the
campus of Lehigh University, Bethlehem, PA, June 3-8, 2018. All courses, lecturers, and
instrumentation will be together for what promises to be a phenomenal week! Course offerings
include: SEM and X-ray Microanalysis • Introduction to SEM and EDS for the New Operator • Focused
Ion Beam Instrumentation and Applications • Problem Solving: Interpretation and Analysis of
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Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register
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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 09:54:08 -0600
Subject: [Microscopy] viaWWW:Need Fiji script to subtract background noise

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Guobin Hu {gb_hu-at-yahoo.com}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}



Hi microscopy subscribers,

We recently purchased an EMS T150 ES for carbon coating. Originally we had a standard chamber. The
coating was very inconsistent. We contact EMS and EMS sent us an extended height chamber and advised
us to control the coating by monitoring the FTM (Film Thickness Monitor) reading instead of by
editable profiles. It does make the coating more controllable. However, the results are still not
consistent.  For example, I coated carbon of 9 nm by FTM and also carbon of 12.5 nm by FTM. The 9 nm
thick carbon actually looked thicker than 12.5 nm. I would appreciate it if users here can share
your experience with me how to coat carbon with consistent and accurate thickness. On the other
hand, I would also appreciate it if you can make recommendations on carbon coaters.

Thanks,

Guobin


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Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll

Organization: Winthrop University

Title-Subject: [Filtered] Need Fiji script to subtract background noise

Message: Due to the large number of images and insufficient amount of ram, I am unable to load both
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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:33:14 -0600
Subject: [Microscopy] Fwd: Water chiller for Hitachi SEM.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Valery Ray {vray-at-partbeamsystech.com}

Hi Ravi,

Check cooling water requirements of the SEM (flow, dissipated power) and Google for compatible
chiller - there are literally hundreds if not thousands outlets selling them online, starting with
amazon.com and zoro.com and ending with ebay.com and aliexpress.com; chiller for running SEM could
be found with price tags below US$1,000.
Valery

Valery Ray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com


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*To:* vray-at-partbeamsystech.com
*Sent:* Thursday, January 11, 2018 9:49 AM
*Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.




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-------- Forwarded Message --------

X-from: McClintock, Joel {jmcclin-at-uky.edu}

Ravi,


This only needs a flow of 1-1.5 liters/minute. Not really very much. Short term, tap water supply
works fine. Risk of condensation and corrosion over time. Constantly running water, which no one
likes as well.


What does flow sensor in back of misroscope indicate is the flow? YOu can change the sensor point on
that flow gauge.


Pretty sure all you are cooling is the Diffusion pump. Think 700 Watt heater. I know the Neslab
CFT33 is sufficient. Specs for it are "950 Watts at 20C 3240 BTU/hr at 20C 817 Kcal/hr at 20C".
The CFT25 model may be not sufficient. The oldHaskris model RO33 will also work. You can find it's
specs. Think mostany chiller with similar specs will work.


Joel

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*Sent:* Thursday, January 11, 2018 10:56:19 AM
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*Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.



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-------- Forwarded Message --------

X-from: Crane, Dan - OSHA {Crane.Dan-at-dol.gov}


I have a Hitachi S3500-N and have used a Haskris R-033 water cooled chiller for it. It was the
recommended chiller by Hitachi. While possibly not the least expensive alternative, it has worked
well for us. You can always call Hitachi High Technologies America, Inc. service department and see
what they recommend.

We have used a variety of different chillers for our other microscopes and instruments, TEM, ICP,
and ICP-MS. Each has to be matched to the power load of the instrument so that they are adequate and
are not overworked so that they fail too soon.

The trick to know with SEM or TEM especially is that the chiller has to provide a very constant
temperature with no pulsation. Temperature is important for magnification stability and absence of
pulsation is essential for imaging and resolution. Not every chiller can provide either or both.
You may not have seen any problems with city water for pulsation or for rapid temperature variance
because of the nature of the source. However, when you go to a closed loop chiller, such problems
can surface and be problematic, really degrading images.

You will need to look at the installation section of the S3500-N instrument and it will give the
temperature and flow requirements for the tool. You can then go to Haskris, or any of the other
suppliers and see what they can do.

Be very careful of used chillers. Pumps and motor-pump interfaces have a lot of wear, might start to
pulse, and might simply give out with no warning. (Been there, done that.) Also, I just had a
compressor develop what can only be called a fistula between the cooling water and the Freon lines.
(We have relatively corrosive tap water.) This was an age-related failure.

I hope that this helps.
Dan Crane

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday,
January 11, 2018 9:13 AM
To: Crane, Dan - OSHA




-------- Forwarded Message --------

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Ravi,

A Haskris chiller will work well - you can get these in air-cooled versions.
As for used … I don’t know. But it’s worth checking your university’s HVAC people. If they don’t
have one hanging around, there might be one available surplus.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab






Email: ravi.thakkar369-at-gmail.com Name: Ravi

Organization: Kansas State University

Title-Subject: [Filtered] Water chiller for Hitachi SEM.

Message: We have Hitachi S3500-N SEM. A chilled water from building supply was used to run the
machine, but now pressure got dropped and we are not getting sufficient flow rate to keep machine
running.I don't see chances of water pressure get increased.
1. Could you guys please suggest what could be the probable suggestion ?
2. Any suggestion, which recirculating chiller will work for Hitachi S3500-N SEM? 3. If you guys can
suggest any source from where we can get used chiller as our budget is limited.
Note : We do not have service contract.
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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:34:10 -0600
Subject: [Microscopy] viaWWW:Balzers BAF 301 freeze fracture consumables source

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X-from: randy-nessler-at-uiowa.edu

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Email: randy-nessler-at-uiowa.edu Name: Randy Nessler

Organization: University of Iowa

Title-Subject: [Filtered] Balzers BAF 301 freeze fracture consumables source

Message: I'm wondering if there is a source for the following items used in our Balzers BAF 301
freeze fracture apparatus:
Bal-Tec BU 020 023-T tungsten filaments
Platinum pellets BD481505 (Balzers part number)
Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number)
Balzers Union BD 484 058 carbon rods

Those part numbers are off some I have on hand, but I hope to add to my inventory.
Searching the internet, I saw the platinum inserts listed as BU 006 103-T, carbon rod for PT/C
evaporation as BU 006 101-T and the carbon rods as BU 006 115. Another listing was "carbon set" BU
020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in a publication rather
than vendor inventory.
Thanks for any help in advance,
Randy


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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:35:19 -0600
Subject: [Microscopy] viaWWW:Seeking Applications Scientists for In Situ TEM in China

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From: wij.muss-at-aon.at
Date: Sat, 13 Jan 2018 15:53:07 -0600
Subject: [Microscopy] Re: Balzers BAF 301 freeze fracture consumables source

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Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] Postdoctoral Position at ORNL on advanced and in situ STEM

Message: There is a postdoctoral position open at ORNL in the Materials Science and Technology
Division on Advanced and in situ STEM (nanomechanics and catalysts)

Qualified candidates can apply through the following link:
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then search for the "Postdoctoral Research Associate in Advanced STEM Characterization / NB50650181"
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Purpose
We are seeking a Postdoctoral Research Associate to focus on the mechanical behavior of materials at
the nanoscale level,
investigate the morphological changes of catalysts during their “life-cycle,” and work with a team
to develop advanced
electron microscopy analysis methods. While this position is based in our Electron Microscopy (EM)
group, you'll collaborate
with a diverse group of experimentalists, theorists, and data scientists across different
organizations within Oak Ridge
National Laboratory (ORNL). This position resides within the Materials Science and Technology
Division (MSTD), Physical
Sciences Directorate at ORNL.

OVERVIEW: As a postdoc, you will support the two projects indicated above while advancing data
acquisition and data
analysis methods for electron microscopy. The mechanical research will be directed toward the
correlation of composition
and crystallographic orientation with mechanical response at the nanoscale level using in situ
nano-compression and
scanning transmission electron microscopy (STEM). The catalytic research will focus on analyzing
structural changes from
the atomic to mesoscale within catalysts and correlating structure, porosity, and compositional
changes with applied
synthesis and other applied treatments/reactions. To do this, you’ll use aberration-corrected
scanning transmission electron
microscopy (STEM) and imaging and analytical microscopy techniques, such as electron energy loss
spectroscopy (EELS)
and energy X-ray dispersive spectroscopy (EDS). You'll also work with a multidisciplinary research
team to develop electron
microscopy characterization techniques suitable for the catalyst and quantitative data analytic
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From isendana0-at-gmail.com Sat Jan 13 00:41:26 2018
Return-Path: {isendana0-at-gmail.com}
Received: from gmail.com (hn.kd.ny.adsl [115.59.74.248] (may be forged))
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id w0D6fPWk002629
for {microscopylistserverarchive6-at-microscopy.com} ; Sat, 13 Jan 2018 00:41:25 -0600
Message-ID: {A896EEE0.5D0F9CC5-at-gmail.com}

Greetings!
Dear Randy,

this is what I found:
ok: page document from 2009.... not knowing whether Stacey Kirsch has
ready or in stock your requested consumables
https://www.emsdiasum.com/microscopy/products/brochures/2009/ems2_09.pdf
p.8.:
Our facility is equipped to handle the following Manufacturers:
} } Balzers/Baltech
email: sgkcck-at-aol.com or stacie-at-ems-secure.com

or:
http://www.highland-scientific.com/balzersbn.html
[NB: ok, This page last updated at 11.13 on Thursday, 14 November 2013]
Contact details:
Highland Scientific
Unit 20, Bedford Business Centre
Mile Road
Bedford
MK42 9TW
England
Tel. + 44 (0)1 234 216 636
Fax. + 44 (0)1 234 271 991
Sales-at-Highland-Scientific.com


As of 25.02.2008: LEICA Microsystems buys / bought BAL-TEC in
Liechtenstein (sorry only in German)
http://www.chemie.de/news/78525/erneuter-firmenerwerb-durch-leica-microsyste
ms-bal-tec-in-liechtenstein.html
so it might be at least possible to find the rep-site of LEICA Microsystems
IOWA or USA and ask them about...


Last but not least (I was able some years ago also to help another
US-colleague to order parts and consumables for former BALZERS - then
BAL-TEC products now BALTIC PRAEparation.e.K.:
Cf: http://www.baltic-praeparation.de/ (unfortunately I found only a
German webpage...
Claudia Kster
Baltic Prparation e.K.
Koppelheck 34b
D-24395 Niesgrau
Telefon: 0 46 43 / 18 65 43
Telefax: 0 46 43 / 18 65 54
Mobil: 0172 / 626 44 55
Email: baltic.praeparation-at-t-online.de

I've written to them using their the php form and asked a preliminary
question for you...and I am sure they will answer your request written in
English...

Best wishes to you and yours,
Wolfgang

=======================================================
MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA

Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

Former Secretary and (until June2017) Board Member of the

SCUR
{The Society for Cutaneous Ultrastructure Research}
The Skin Imaging Society { www.scur.org }
====================================================================


Von: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Gesendet: Freitag, 12. Januar 2018 02:03
An: wij.muss-at-aon.at
Betreff: [Microscopy] Balzers BAF 301 freeze fracture consumables source
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Email: randy-nessler-at-uiowa.edu Name: Randy Nessler
Organization: University of Iowa
Title-Subject: Balzers BAF 301 freeze fracture
consumables source
Message:

I'm wondering if there is a source for the following items used in our
Balzers BAF 301 freeze fracture apparatus:
Bal-Tec BU 020 023-T tungsten filaments
Platinum pellets BD481505 (Balzers part number)
Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number)
Balzers Union BD 484 058 carbon rods

Those part numbers are off some I have on hand, but I hope to add to my
inventory.
Searching the internet, I saw the platinum inserts listed as BU 006 103-T,
carbon rod for PT/C evaporation as BU 006 101-T and the carbon rods as BU
006 115. Another listing was "carbon set" BU
020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in
a publication rather than vendor inventory.
Thanks for any help in advance,
Randy

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22, 21 -- From wij.muss-at-aon.at Sat Jan 13 15:53:07 2018
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From: CGorman-at-hookecollege.com
Date: Mon, 15 Jan 2018 11:53:08 -0600
Subject: [Microscopy] Scanning Electron Microscopy Training

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Hooke College of Applied Sciences, located in Westmont, IL, is offering a Scanning Electron Microscopy short course March 5-9, 2018.

In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.

For further SEM training details and registration information, please follow the link below:

https://www.mccrone.com/scanning-electron-microscopy-course

Best regards-
__________________________________________________
Chris



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From: microscopy.listserver-at-gmail.com
Date: Tue, 16 Jan 2018 08:54:07 -0600
Subject: [Microscopy] viaWWW:Help with ASPEX 3025 SEM

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Email: jdykes-at-wvc.edu Name: Jeff Dykes

Organization: Wenatchee Valley College

Title-Subject: [Filtered] Help with ASPEX 3025 SEM

Message: Hello All,

My department just received a Scanning Electron Microscope and we are looking for some assistance.
We are in need of:

-An operators manual that could be copied and sent to us. It is for a ASPEX 3025 SEM.
-Guidance or protocols to prepare (fix) samples such as plants, bacteria, insects.
-Any other documents, tips, etc.

Thank you,

Jeff Dykes
Science Faculty
Wenatchee Valley College
116 West Apple
Omak, WA 98841 (USA)

jdykes-at-wvc.edu
509 422-7876

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jan 2018 07:22:28 -0600
Subject: [Microscopy] viaWWW:Postdoc - Advanced TEM of Steel/Thin Films

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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoc - Advanced TEM of Steel/Thin Films

Message: Postdoc position in Advanced Microscopy of Steel Microstructure and Thin Films - Position
Available Now.

A postdoctoral position is available in the performance of two distinct projects. The researcher
will use the state-of-the-art aberration-corrected TEM's at the Canadian Centre for Electron
Microscopy (ccem.mcmaster.ca) located at McMaster University, Hamilton Canada. The CCEM is a
world-class imaging facility and is equipped with 4 TEM's, including two aberration-corrected
microscopes (FEI Titans).
The first project is to study precipitation mechanisms of Nb-rich steels after hot rolling. This
work requires knowledge of chemical imaging, diffraction methods, and an understanding of strain
measurement. The second project is the study of the thin film growth process, from 2-D materials up
to ALD-grown films. This requires high-resolution imaging (TEM and STEM), EELS, and EDS.

The postdoc will be part of the Bassim research group, with a strong collaboration with Prof.
Zurob's research group.

Applicants should have a strong background in electron microscopy, preferably with
aberration-corrected (S)TEM experience. Additional background in metallurgy or thin films is a
bonus. Ability to work in a team, mentor graduate students, as well as excellent verbal and written
communication skills are expected.

McMaster University is located in Hamilton, Ontario, within a 45 minute drive from downtown Toronto,
which a world class city and 45 minute drive to Niagara Falls.

To apply, please send a cover letter, curriculum vitae including references, and 1-2 relevant
published papers to the e-mail below.

Nabil Bassim
Associate Professor
Department of Materials Science and Engineering, JHE 258
McMaster University
1280 Main Street West
Hamilton, ON L8S 4L8
CANADA
nbassim.mcmaster.ca
bassimn-at-mcmaster.ca

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jan 2018 07:23:09 -0600
Subject: [Microscopy] viaWWW:Solid State X-ray Spectrometry at 50 Years: Session A11

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Email: paul.carpenter.epma-at-gmail.com Name: Paul Carpenter

Organization: Washington University

Title-Subject: [Filtered] Solid State X-ray Spectrometry at 50 Years: Session A11 Microscopy &
Microanalysis 2018

Message: Dear Microscopists and Microanalysts,
It is our pleasure to inform you of: Solid State X-ray Spectrometry at 50 Years, session A11 at the
2018 Microscopy & Microanalysis Meeting, which will be held Aug. 5-9, 2018 in Baltimore, MD.

This session will concentrate on the diverse advances in hardware, software, and applications of
energy-dispersive spectrometry in the last 50 years.

We are particularly interested in applications from the biological and materials communities,
especially from students and young scientists.

The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018:
https://www.microscopy.org/MandM/2018/

Solid State X-ray Spectrometry at 50 Years

Session chairs: Paul Carpenter, Edward Vicenzi, Kate Burgess, Nicholas Ritchie

50 years ago, Fitzgerald, Keil, and Heinrich published the first results obtained from an
energy-dispersive x-ray spectrometer (EDS) in Science. From identification of unknown materials, to
compositional mapping and quantitative microanalysis, EDS has advanced our understanding of an
enormous range of materials and is used worldwide in microanalysis and microscopy laboratories. The
symposium will link historical and technical developments of solid state x-ray instrumentation, data
processing, applications, and emerging detection systems. Perspectives on the developments in EDS
from a technological and educational perspective will be featured, including invited and contributed
presentations from the inventor, vendor, and scientific communities.

o State-of-the-art solid state x-ray detector instrumentation
o Soft x-ray and light element analysis in the field emission SEM
o Nano to atomic resolution microanalysis by STEM
o Quantitative analysis methods - limits and limitations
o Spectrum imaging and data processing
o Complementary methodologies including micro-XRF, combined WDS-EDS, XRD, and EBSD

Paul Carpenter, Washington University

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jan 2018 07:23:57 -0600
Subject: [Microscopy] viaWWW:Lorentz microscopy - position

Contents Retrieved from Microscopy Listserver Archives
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Email: klemari-at-fzu.cz Name: Mariana Klementova

Organization: Institute of Physics of the Czech Academy of Sciences

Title-Subject: [Filtered] Lorentz microscopy - position

Message: Dear colleagues,

we are currently looking for a microscopist who would be able to introduce Lorentz microscopy to our
group at the Institute of Physics. We have a Tecnai F20 X-twin with a Lorentz lens. We have got
funding for the position for 6 months. The position will be available from June 1, 2018.

If you are interested please contact me by January 31, 2018.

With best regards,

Mariana Klementova

************************************************
Mariana Klementova
************************************************
Institute of Physics of the CAS, v.v.i.
Na Slovance 1999/2
CZ-182 21 Praha 8
Czech Republic
************************************************
tel. : +420 - 266 05 2612
email: klemari-at-fzu.cz
************************************************

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From: Thomas.Kremer-at-sgs.com
Date: Fri, 19 Jan 2018 14:13:48 -0600
Subject: [Microscopy] SEM - Using a Wig-L-Bug grinder

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Email: kandi-at-isearchbio.com Name: Kandi Williams

Organization: K.L. Williams & Associates

Title-Subject: [Filtered] Entry level microscope sales - San Francisco and Seattle

Message: We are currently working with a major manufacturer of laboratory microscopes and related
optical products to assist them in filling a San Francisco and a Seattle based entry level
microscope sales positions. We are looking for a young, energetic scientist with a year or two of
post doc experience, or several years post graduate lab management experience, with heavy confocal
experience – using, teaching, troubleshooting. This would be an excellent first step off the bench
into industry.
The company will provide excellent training and mentorship and a solid career path. First year
salary package is anticipated to be around $100k plus the company provides a monthly car allowance,
covers all business and travel expenses and has an excellent benefits package. Overnight business
travel for both territories is low, maybe 2 to 3 nights/month, primarily for meetings and training.
Please note that the company is unable to provide any sort of visa sponsorship at this time. Take care,
Kandi Williams

K.L. Williams & Associates
P.O. Box 5421
Auburn, CA 95604-5421
(530) 885-3693
kandi at isearchbio.com


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From koepketemi17-at-gmail.com Thu Jan 18 09:23:47 2018
Return-Path: {koepketemi17-at-gmail.com}
Received: from gmail.com (hn.kd.ny.adsl [115.59.74.248] (may be forged))
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Message-ID: {3BE428CD.679FDF8D-at-gmail.com}

Hello,
I recently purchased a Wig-L-Bug grinder/mixer, an agate vial and an adapter to hold the vial. I am at a loss as to how the vial is held by the adapter and the adapter certainly does not fit on the arms of the grinder/mixer. I made three attempts at contacting the manufacturer's technical support but to no avail. Hence this plea for help. If any of you have this setup, I appreciate your input. I can share photos of the parts if that would help.

BTW, the purpose for the agate vial is for grinding ashed materials that often contain silicates.

Tom Kremer
SGS-IPS Testing
Appleton, WI
Information in this email and any attachments is confidential and intended solely for the use of the individual(s) to whom it is addressed or otherwise directed. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of the Company. Finally, the recipient should check this email and any attachments for the presence of viruses. The Company accepts no liability for any damage caused by any virus transmitted by this email. All SGS services are rendered in accordance with the applicable SGS conditions of service available on request and accessible at http://www.sgs.com/en/Terms-and-Conditions.aspx


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From: microscopy.listserver-at-gmail.com
Date: Sun, 21 Jan 2018 22:31:09 -0600
Subject: [Microscopy] viaWWW:Vac check connection failure, Zeiss Leo SEM

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Email: mplattsc-at-gmail.com Name: Michael Platt

Organization: University of South Carolina

Title-Subject: [Filtered] Vac check connection failure, Zeiss Leo SEM

Message: I have been working on this issue, along with an L-REM failure off and on for a while and
have gotten nowhere. I have been concentrating on the L-REM failure but now I want to look harder
into trying to solve the Vac Check Connection fail. Those failures show up during the power up
initialization. First, what does that really mean? Not that it has anything to do with the failure
but the backing pump and turbo pump both come on and I believe the turbo comes up to speed, solid
green light on the controller. The vacuum gauge's green lamp is also on.
Regarding previous suggestions I have check every fuse I could find, all good. All fiber optic
cables appear to be OK on the L-REM and associated units. Tests indicate the power supply failures
on the EO and PU boards but where exactly are they as I could find only numeric id's for all the PC
boards.

Suggestions?

Michael Platt


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13, 53 -- Subject: viaWWW:Vac check connection failure, Zeiss Leo SEM
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From: ajhall-at-prairienanotech.com
Date: Mon, 29 Jan 2018 12:36:10 -0600
Subject: [Microscopy] Re: Ask a Microscopist - embedding pigments & powders for microtomy

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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University

Title-Subject: [Filtered] The Rockefeller University seeks Electron Microscopy Specialist

Message: Message: Electron Microscopist, The Rockefeller University, New York, NY

The Rockefeller University, a premier biomedical research institution, seeks a Research
Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).

The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of
biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells
and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is
equipped with three transmission electron microscopes, a conventional and a serial block-face
imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit.
(http://www.rockefeller.edu/emrc/)

The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations,
including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering
supplies, interacting with vendors, and administrative support for office duties, including center
billing. The position also entails specimen preparation, including negative staining, ultrathin
sectioning, and immunolabeling, operation of the microscopes and associated equipment, training
users, as well as consulting scientists on the design of experiments, data processing/analysis,
interpretation of results, and informing users on the latest methodology through familiarity with
relevant literature.

The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology,
bioengineering or a related field and must have a minimum of 5 years of hands-on experience in
electron microscopy. A strong background in computation would be a plus. Must have strong
communication skills, and the ability to work collaboratively in a team as well as independently on
a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.

We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to
this job, click the following URL, click on 'staff opportunitiesf and enter keywordeIRC20449f or
eElectron Microscopistf: http://www.rockefeller.edu/hr/career.php

The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.

Regards,
Hiro
------
Kunihiro Uryu, Ph.D., Research Assistant Professor
Director of Electron Microscopy Resource Center (EMRC)
RRB Rm120
The Rockefeller University
1230 York Ave., Box 230
New York, NY 10065


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Email: lamt-at-si.edu Name: Thomas Lam

Organization: Smithsonian Museum Conservation Institute

Title-Subject: [Filtered] P08 Spectroscopic and Imaging Studies in Heritage Science

Message: It is our pleasure to inform you there will be a Spectroscopic and Imaging Studies in
Heritage Science symposium, session P08 at the 2018 Microscopy & Microanalysis Meeting, which will
be held Aug. 5-9, 2018 in Baltimore, MD.

The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018:
https://www.microscopy.org/MandM/2018/

P08 Spectroscopic and Imaging Studies in Heritage Science

The application of microscale and nanoscale characterization techniques to the examination of
cultural heritage materials has greatly enhanced our understanding of the processes that formed, and
subsequently transformed those materials to their present state. Understanding the chemistry and
morphology of heritage materials from the macro/mesoscopic scale to the microscale is of critical
importance for our increasingly deeper levels of knowledge of the interaction between objects and
their environment. This symposium will include invited and contributed presentations from students,
conservators, conservation scientists, researchers, and those from other disciplines who have an
interest in the preservation of cultural heritage.

o Emerging methods for microscale and nanoscale examination in culture heritage
o Case studies on historic and prehistoric materials
o Non-invasive and minimally-invasive imaging and analysis methods
o Kinetics of light fastness using microfadeometry
o Synchrotron- and neutron-based studies
o New approaches to protective coatings for heritage objects
________________________________________

Students, postdocs, and technical staff
All full-time students enrolled at accredited academic institutions and all full-time postdoctoral
fellows are eligible for meeting award support. High school, undergraduate, and graduate students
are encouraged to apply. Applicants are not required to be members of the sponsoring societies.
Additionally, full-time technologists are eligible for support as well if the applicant is a member
of the sponsoring society, current in his or her dues for the year of the meeting.

We look forward to seeing you at the Baltimore inner harbor next year!
On behalf of the symposium organizers,

Edward P. Vicenzi (Smithsonian Institution)
John Mansfield (University of Michigan)
Thomas Lam (Smithsonian Institution)


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Organization: Northern California Society for Microscopy

Title-Subject: [Filtered] NCSM Spring Meeting in Pleasanton, CA

Message: Northern California Society for Microscopy announces its spring meeting on March 27 at the
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Name: Janine Hernandez
School: San Joaquin Delta College
Grade/Education Level: Undergraduate
Location: Stockton, CA
Email: janine.hernandez182-at-gmail.com

(Listserve - I apologize - I think I did the reply incorrectly at first)

Hi Janine,

Powders and pigments likely do not require encapsulation. It's usually
much easier to place them on a supporting grid directly for imaging.
This of course assumes that the size of the powder or pigment is small
enough for electron transmission. Often it's worth it just to check
incase it is possible; which makes sample prep fairly easy.

There are a few methods that could be used to prepare these- one that
I've had some success with fine powders in the past is as follows: place
an amount of the powder into isopropanol, ultrasonicate to break up
agglomerations, dip a holy-carbon grid into the liquid, pull it out, and
allowing the grid to air dry. If there was a small enough amount, but
enough to be finely dispersed in the alcohol, you will pick up some on
the grid. This grid, when dried, can then be imaged with the powders
suspended on the holy-carbon. It may take a few trials to get enough
from the alcohol suspension. Another method that has worked for me in
the past (assuming the powder is well separated) is a very light dusting
of the powder mid-air above the grid (allowing some of the powder to
fall down onto the grid on the table). This one is less likely to obtain
separated powders that are small enough to image, but I have seen some
success with it as well in the past. (Use of a clean fine artist paint
brush to flick the powder into the air above the grid may work here.)
Please be careful not to allow the alcohol with the powder or the powder
in air to get on your skin or to be breathed in. Some solvents (acetone
notably) allows chemicals to enter through the skin, and we are learning
that nanopowders are not healthy for you to breathe in as well.

I hope this is helpful. You'll likely get a number of great answers
from the list-serve and I'm looking forward to learning from their
answers also!

Wishing you a grand time exploring small worlds!
-Allen J. Hall
Prairie Nanotechnology
www.prairienanotech.com

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From: korinek-at-mcmaster.ca
Date: Mon, 29 Jan 2018 15:19:14 -0600
Subject: [Microscopy] Job Opening: Senior Postdoctoral Fellow in the Canadian Centre for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Senior Postdoctoral Fellow in the Canadian Centre for Electron
Microscopy

The Canadian Centre for Electron Microscopy (CCEM) is a national
facility located at McMaster University, in Hamilton, ON, Canada.
The CCEM operates four TEMs, including two FEI Titan TEMs, equipped
with image
and probe correctors and monochromators, as well as a Quantum GIF
equipped with
K2 direct electron detector for spectroscopy.

We are seeking a skilled and motivated researcher to perform a variety
of
imaging and analysis experiments supporting the needs of academic and
industrial
users, including consulting, planning of experiments, data
processing and interpretation. An important role of this position is
the training of users on the
instruments.

Candidates should have a PhD in Physics, Material Science/Engineering,
Chemistry or
a related field with demonstrated hands-on experience
using transmission electron
microscopes and EDS/EELS, preferrably Titan TEMs. Strong communication
skills and the ability to develop successful data acquisition and
processing strategies will be essential in this role. Experience in
data processing and scripting in Python or Matlab, as well as a
demonstrated
track record of research and interactions with users would be an asset.

To apply, please send your cover letter and resume to korinek-at-mcmaster.
ca


--
Dr. Andreas Korinek

Manager

Canadian Centre for Electron Microscopy
Brockhouse Institute for Materials Research
McMaster University
1280 Main Street West, Hamilton ON Canada, L8S 4M1
phone: +1 905-525-9140 ext 20400


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From: microscopy.listserver-at-gmail.com
Date: Tue, 30 Jan 2018 08:05:06 -0600
Subject: [Microscopy] viaWWW: Zeiss 10C available

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Email: psicurello-at-ucsd.edu Name: Paula Sicurello

Organization: UCSD

Title-Subject: [Filtered] Zeiss 10C available

Message: Please post for me this Zeiss 10C that we are giving away.

Hello Everybody,

Free to a good home.

Is anyone interested in a vintage Zeiss 10C? This scope is in working condition and has been
lovingly maintained. It comes with a side mounted Gatan ES1000W camera. The purchaser will be
responsible for the costs associated with the disassembly and removal.

Please contact me if you are interested.

Sincerely,

Paula Sicurello, HTL (ASCP)

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

psicurello-at-ucsd.edu
P: 619-583-2872

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health
200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872


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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Jan 2018 09:10:45 -0600
Subject: [Microscopy] viaWWW:Fluorescent Microspheres

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Email: holtzcll-at-mail.nih.gov Name: Lynne Holtzclaw

Organization: NIH

Title-Subject: [Filtered] Fluorescent Microspheres

Message: Does anyone have a proven protocol for coating #1.5 coverglass with a layer of fluorescent
microspheres? (Tetraspeck, 0.1)

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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Jan 2018 09:11:26 -0600
Subject: [Microscopy] viaWWW: ETEC Autoscan SEM circuit diagrams needed

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Email: cjbray-at-es.utoronto.ca Name: Colin Bray

Organization: Earth Science, Univ. of Toronto

Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed

Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the
vacuum system driver board. he has circuit diagrams for most of the other units but naturally, not
the one he needs. Can anyone help?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Feb 2018 15:01:25 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: ETEC Autoscan SEM circuit diagrams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues - to commemorate the decade of PFM conferences, I have
made the integrated set of slides (~350) on the principles and
applications of piezoresponse force microscopy and spectroscopy based on
tutorials on ~20 PFM workshops and conferences over last decade.

There are 7 parts, including:
1. Introduction to PFM and Nanoscale electromechanical phenomena
2. Contact mechanics and image formation in PFM
3. Cantilever dynamics in PFM
4. PFM of ferroelectric materials and local polarization switching
5. Switching spectroscopy piezoresponse force microscopy
6. Advanced time- and voltage spectroscopies in PFM
7. PFM in polar and nonpolar liquids

If interested, please contact me directly at sergei2-at-ornl.gov
{mailto:sergei2-at-ornl.gov} . Also, please forward this information to your
colleagues interested in PFM.

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From westelle638-at-gmail.com Thu Feb 1 01:16:29 2018
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Ken Converse used to support ETECs, the bits of contact info I have are:

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME  04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz {mailto:kenconverse-at-qualityimages.biz}
qualityimages.biz

Quality Images, /Ken Converse/, Maine, from NC to ME to OH, SEM, Amray//Etec//Any, (717) 456-5491

Good luck!
Valery
Valery Ray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com


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*To:* vray-at-partbeamsystech.com
*Sent:* Wednesday, January 31, 2018 9:08 AM
*Subject:* [Microscopy] viaWWW: ETEC Autoscan SEM circuit diagrams needed




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Email: cjbray-at-es.utoronto.ca {mailto:cjbray-at-es.utoronto.ca} Name: Colin Bray

Organization: Earth Science, Univ. of Toronto

Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed

Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the
vacuum system driver board.  he has circuit diagrams for most of the other units but naturally, not
the one he needs.  Can anyone help?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Feb 2018 15:02:44 -0600
Subject: [Microscopy] viaWWW:Beads size and intensity

Contents Retrieved from Microscopy Listserver Archives
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Email: tstern-at-princeton.edu Name: Tomer Stern

Organization: Princeton University

Title-Subject: [Filtered] Beads size and intensity

Message: Hi all,

I'm using a MuVi-SPIM, similar to the Luxendo model.

To register the different views and also to infer the PSF prior to deconvolution I'm using beads,
which are scattered around the sample - inside the agarose - and are being scanned at the same time
as the sample.

I have two questions:

1. Is the intensity of the beads relative to the intensity of the marker inside the tissue important
for the inference of the PSF? Meaning, is it better to use beads with intensities that are similar
to the sample, or should their intensity be higher / lower / it doesn't really matter?

2. As far as I know the preferred diameter of the bead is one that is a bit smaller than the size of
the voxel in the XY plane, right? if so, then if I know that I'm going to use a 2x2 binning simply
by averaging every 2x2 block in each slide, should I still use the same size of beads, or should it
match the new size of the 'binned' - enlarged - pixels?


Thanks in advance!
Tomer


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From: microscopy.listserver-at-gmail.com
Date: Tue, 6 Feb 2018 07:26:12 -0600
Subject: [Microscopy] viaWWW:Gatan dimple grinder

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Berkeley Lab’s Molecular Foundry Division has an opening for a
Scientific Engineering Associate or Sr. Scientific Engineering
Associate. The Molecular Foundry is a Department of Energy-funded
nanoscience research facility that provides users from around the
world with access to cutting-edge expertise and instrumentation in a
collaborative, multidisciplinary environment. As one of the seven
facilities in the Molecular Foundry, The National Center for Electron
Microscopy (NCEM) serves a broad user community in materials science
by supplying unique instrumentation, expert advice, assistance, and
training in advanced electron microscopy techniques.

Diversity, equity, and inclusion are core values at the Molecular
Foundry. Therefore, we seek candidates whose research, past
employment, or service has prepared them to contribute to our
commitment to diversity and inclusion. Our excellence can only be
fully realized by staff who share our commitment to these values.
Successful candidates for our staff position will demonstrate either
potential to contribute or evidence of a commitment to equity and
inclusion.

This position provides highly specialized expertise, consultation, and
contributions to project process and knowledge in support of in situ
transmission electron microscopy (TEM) experimentation and TEM sample
preparation. This includes the development and optimization of
techniques for preparing high resolution TEM samples from a wide
variety of materials and for in situ TEM investigations, user
assistance and training, technical proposal evaluation, and
collaborations on scientific projects. The position is responsible for
scheduling, maintenance, operation and technical development of the
sample preparation laboratories and in situ TEM experimental
equipment. Will provide resolution to a diverse range of moderately
complex technical problems and participate in, and in some cases lead,
experimental methodology development and project execution.
Classification will depend upon the applicant's level of skills,
knowledge, and abilities.

For further details and to apply please go to the following link:

http://m.rfer.us/LBLYNuJJ


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From anittiff04obdiq-at-gmail.com Thu Feb 1 22:38:55 2018
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Email: jflaci-at-ms.sapientia.ro Name: Lszl Jakab-Farkas

Organization: Sapientia EMTE

Title-Subject: [Filtered] Gatan dimple grinder

Message: Hello,
I'm from a small university in Transsylvania,
we are running a microscopy lab with a vintage Jeol 100U and an JSM-5200.

We would need in our laboratory for sample preparation a Gatan Dimple
Grinder.

Can You point me to a reliable source for
cheaper second hand equipment?

Cheers

JFL



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From: microscopy.listserver-at-gmail.com
Date: Wed, 7 Feb 2018 07:32:30 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW:Postdoctoral_Fellow_=c2=96_In_situ_cryo-EM?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

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Email: carsten.sachse-at-embl.de Name: Carsten Sachse

Organization: EMBL Heidelberg

Title-Subject: [Filtered] Postdoctoral Fellow – In situ cryo-EM

Message: Dear list members,

In my lab, a position of in situ cryo-EM in the area of structural cell
biology is available. I am still looking for a candidate with experience
in cryo-ET/EM and/or FIB-SEM for the following project:
http://s.embl.org/HD01243

In case you have further questions, please do not hesitate to contact
me. Closing date for applications is Feb 16.

Best wishes,


Carsten
____________________________________________________________________________________
Dr. Carsten Sachse Group Leader
European Molecular Biology Laboratory Meyerhofstr. 1
69117 Heidelberg
Germany
email: carsten.sachse-at-embl.de
http://www.embl.de/research/units/scb/sachse/
http://www.sachse.embl.de

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From: frank_karl-at-ardl.com
Date: Wed, 7 Feb 2018 13:51:31 -0600
Subject: [Microscopy] Beam always on

Contents Retrieved from Microscopy Listserver Archives
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Hello Everyone,
I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.

Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!


Thanks!!!

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: Duane.Harland-at-agresearch.co.nz
Date: Wed, 7 Feb 2018 14:45:25 -0600
Subject: [Microscopy] Beam always on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank,

I've had a similar issue with our FEI Morgagni 267D (for most purposes similar to the CM12). I'd bet the wehnalt assembly is almost identical.
For us it was almost certainly a filament break in such a way that it was shorting across the wehnalt. I say "almost certainly" because in our case this happened at almost the same time that a bunch of electronics issues occurred and so we had mixed symptoms. But replacing the filament (which we had only just replaced as part of our diagnosis of elimination), did the trick for us.

At least it is a simple check.

Good luck,
Duane

Duane Harland
Senior Scientist
Food & Bio-based Products
T +64 3 321 8710
Based at Lincoln Research Centre Campus agresearch.co.nz
New Zealand

-----Original Message-----
X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com]
Sent: Thursday, 8 February 2018 9:10 AM
To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}

Hello Everyone,
I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.

Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!


Thanks!!!

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: jacques.faerber-at-ipcms.unistra.fr
Date: Thu, 8 Feb 2018 10:20:26 -0600
Subject: [Microscopy] OM focus knob motorisation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Frank,
This is called Dark Current. It comes from the evaporation of filament material onto the insulator base creating a pathway for electrons to leak to ground, and therefore, creating a current in the filament that causes it to glow with only the kV on. It’s not a problem, but indicates that your filament is old.
Ken

Kenneth JT Livi, PhD
Director, Materials Characterization and Processing Center
Materials Science and Engineering
3400 N Charles Street
Johns Hopkins University
Baltimore, Maryland 21218 USA

} On Feb 7, 2018, at 2:56 PM, frank_karl-at-ardl.com wrote:
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} Hello Everyone,
} I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.
}
} Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!
}
}
} Thanks!!!
}
} Stay safe...........
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}


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From donnedua0-at-gmail.com Wed Feb 7 18:52:23 2018
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Hi all

A collegue in the lab has strong health difficulties with her shoulders
and wrists. She works on a Zeiss Axio Imager D2m with a special stage
and external accessories which leaves only a close access to the
focusing knobs.
So we are looking for a companies which could fit a motorization on the
focus knobs, to have a remote control of them.
Does someone know a (preferably french or europeen) company which could
do this job ? We'll ask Zeiss too, but we have some fear about the price...

Thanks in advance
Jacques

--

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Feb 2018 16:12:55 -0600
Subject: [Microscopy] viaWWW:JEOL 840A SEM video signal

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Email: gary-at-cermetmaterials.com Name: Gary Castelow

Organization: CERMET MATERIALS INC.

Title-Subject: [Filtered] JEOL 840A SEM

Message: Hello all! We randomly lost video signal with our SEM the
other day. Acted as if the filament had blown, but the check gauge is
still reading current when the AC is turned on. Im am by no means an
"electrician" but all connections still look ok going to the viewing
monitor. Is there something fairly easy that goes bad I could check??
Or any other help is greatly appreciated!

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From: microscopy.listserver-at-gmail.com
Date: Fri, 9 Feb 2018 03:08:08 -0600
Subject: [Microscopy] viaWWW:Tungsten filaments

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Email: wschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison
Title-Subject: [Filtered] Tungsten filaments

Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does
anyone have any suggestions for extending filament life? We try and
operate slightly under saturated except for EBSD or EDS mapping. I’ve
heard of “seasoning” the filament but have never seen an actual protocol
or talked with anybody who’s actually done it. Any suggestions would be
appreciated!

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From: diller-at-stefan-diller.com
Date: Fri, 9 Feb 2018 04:19:17 -0600
Subject: [Microscopy] Re: viaWWW:JEOL 840A SEM video signal

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Hello Gary,

did you check your complete video-processing path, like:

- setting the gain of the SE-detector so high to finally see the signal noise (this would state at least a working photomultiplier
/ detector system)

- did you check for the cage voltage switch to be on "positive" ?

- check on HV voltage and cathode heating. Do you see any change by going up in heating?

- centering and e-beam shift potis aligned?

- cathode OK? Current might be a dark currant or mis-functioning high voltage unit

- take out the final aperture and try to find beam...

These are only a few possibilities...


Best wishes,

Stefan



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From: wij.muss-at-aon.at
Date: Fri, 9 Feb 2018 07:10:02 -0600
Subject: [Microscopy] RE: Tungsten filaments [Hitachi S3400N VPSEM, how extending filament life?]

Contents Retrieved from Microscopy Listserver Archives
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Apologize for lengthiness

Bill,
with " } seasoning { a tungsten filament" you perhaps (might) think of slow
and careful -controlled 'heating' of a new filament
(as I have done that with every new filament after mounting in a TEM, a
ZEISS109 with differential pumping system=rotary pump -for RV as well as an
Ion Getter Pump for HV).

IMHO 'slow heating' of the cathode filament before subjecting it to the
"hard" working conditions might be (at least in my experience really 'is' )
of benefit regarding beam stability as well as maintaining your filament's
lifetime.

"Slow heating" means [after change of filament] at least running the new
filament 1-2 hrs 'under-under'-saturated after achievement of an evacuation
optimum of the chamber (not knowing whether you could see anyhow the
filament's image in such a condition like in a TEM on the screen).
You easily would / could see (if you e.g. have permanent vacuum-measurement
monitoring ready) that on starting to "heat"(increase) the filament
(current), the vacuum decreases significantly (at least slightly) because of
"evaporation" of metal & impurities / contaminants.

Then increase filament current a bit more.... and let "equilibrate" vacuum
conditions. You might even find such decreasing vacuum after a third
increasing of the filament heating near the 'saturation' point.

Another helpful requisite also would be to use - without using or even
heating the filament first an ACD (anti-contamination device - if
available) with at least one if not two cycles of cooling first and pumping
the chamber afterwards to get rid of most organic molecules you brought into
the filament chamber by your cleaning and mounting procedures - let warm up
the ACD and pump (RV and HV if possible) again.
According to the specifications of the Hitachi S3400N VPSEM (I found on:
https://www.ntnu.edu/documents/140082/1269041159/S-3400NSpecifications.pdf/6
b94c26f-a9d4-4f36-82b3-7e9eb3603922 ) the possibility of doing so might be
impossible due to the automatic settings of diverse functions but you should
know about those possibilities from practical experience.

Unfortunately a modern VPSEM is not comparable to such an old piece of
equipment like the TEM ZEISS 109 (vintage 1976) I worked with where
maintaining and handling vacuum matters relatively easy...
And, BTW, life time of a tungsten filament always depends also on the
"technical quality" and the handling - when mounting into the chamber -
too....

Best wishes and good luck,
Wolfgang



MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]
A-5020 SALZBURG
sterreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA
Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

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Gesendet: Freitag, 9. Februar 2018 10:29
An: wij.muss-at-aon.at
Betreff: [Microscopy] Tungsten filaments [Hitachi S3400N VPSEM, how
extending filament life?]
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Email: wschneider-at-wisc.edu Name: Bil Schneider
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Title-Subject: Tungsten filaments
Message:

We use tungsten filaments in our Hitachi S3400N VPSEM.
Does anyone have any suggestions for extending filament life? We try and
operate slightly under saturated except for EBSD or EDS mapping.
Ive heard of seasoning the filament but have never seen an actual
protocol or talked with anybody whos actually done it.
Any suggestions would be appreciated!

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From: wesaia-at-iastate.edu
Date: Fri, 9 Feb 2018 12:00:41 -0600
Subject: [Microscopy] viaWWW:Tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
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First, what kind of filament life are you currently getting? If you are close to 100 hours, I would be satisfied. If you are only getting 30, then I would look for a problem.

We got better life when we ran at a consistent voltage and didn't have users doing the saturating. We went from average lifetimes of 25 hours to 80 hours. We also operated just under saturation conditions. We simply turned off the high voltage at the end of the session.

We needed to check saturation and reduce current over time. As the filament thinned, it needed less current to reach the same temperature.

Finally, we ran the filament a little further back from the front of the wehnelt. That lessened brightness but increased life.

Warren
________________________________________
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Email: wschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison
Title-Subject: [Filtered] Tungsten filaments

Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does
anyone have any suggestions for extending filament life? We try and
operate slightly under saturated except for EBSD or EDS mapping. I've
heard of "seasoning" the filament but have never seen an actual protocol
or talked with anybody who's actually done it. Any suggestions would be
appreciated!

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From: oshel1pe-at-cmich.edu
Date: Fri, 9 Feb 2018 16:58:07 -0600
Subject: [Microscopy] FW: Ask a Microscopist - Imaging board needed for a Leo 1525

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Name:karl Hagglund
School:Northwestern University
Grade/Education Level:Graduate
Location:Evanston, IL
US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject}
Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board
Your Question:
Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts.
Thank you,
Karl Hagglund





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From: nmz787-at-gmail.com
Date: Fri, 9 Feb 2018 17:55:35 -0600
Subject: [Microscopy] Re: FW: Ask a Microscopist - Imaging board needed for a

Contents Retrieved from Microscopy Listserver Archives
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Hi Karl,
If you're in a rush, I would start by contacting these after-market providers:
http://www.jcnabity.com/links.htm#Digital Imaging

If you aren't in a rush, I would find an Electrical Engineering
student who wants a long-term project (lots of EE programs have this
as a graduation requirement) and get them to build one for you (and
ideally require they publish their methods openly). They can start
with this for inspiration and basics:

Electron microscope image capture with an oscilloscope
https://www.youtube.com/watch?v=SWVu-qPR-Ws

Electron microscope image capture via microcontroller (with drill bit animation)
https://www.youtube.com/watch?v=ruuxn2u3yao
http://benkrasnow.blogspot.com/2015/08/drill-bit-in-electron-microscope.html

DIY Scanning Electron Microscope - Sources, Costs and References
http://benkrasnow.blogspot.com/2011/03/diy-scanning-electron-microscope_26.html

SEM data files and image generation script
http://benkrasnow.blogspot.com/2014/11/sem-data-files-and-image-generation.html

DIY Scanning Electron Microscope - Image Quality Improvements
http://benkrasnow.blogspot.com/2011/04/diy-scanning-electron-microscope-image.html
http://benkrasnow.blogspot.com/2011/05/diy-scanning-electron-microscope-image.html
http://benkrasnow.blogspot.com/2011/05/diy-scanning-electron-microscope-image_07.html

Best,
-Nathan

On Fri, Feb 9, 2018 at 3:18 PM, {oshel1pe-at-cmich.edu} wrote:
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} Name:karl Hagglund
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} Location:Evanston, IL
} US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject}
} Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board
} Your Question:
} Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts.
} Thank you,
} Karl Hagglund
}
}
}
}
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--
-Nathan


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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 00:14:39 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:Tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: James M. Ehrman {jehrman-at-mta.ca}

Bill,

Do you have a high vacuum gauge on the scope? With both our new Hitachi
(SU3500) and our old JEOL (5600), the "ready" state comes on when the
vacuum is in the high 10^-4 torr range (or even higher if there was a
vacuum leak, outgassing sample, or other problem). Running a filament at
such a poor vacuum will drastically reduce filament life and generally
contaminate the column faster. I insist on having a gauge on any scope I
run, and don't turn the beam on until the vacuum is at least mid 10^-5.
With vacuum gauges under $1000, they'll quickly pay for themselves in
terms of filaments and time lost changing them. I've ranted to both JEOL
and Hitachi that they should make them standard on all scopes. By their
own admission, half of their service calls are vacuum related, and with
a gauge the end user can often find a leak and save a service visit. In
our case with the closest service in Ontario, it's a $2000 flight to get
here. Should be a simple budgetary calculation, but Hitachi says I'm the
only customer in Canada who ever requested a vacuum gauge.

I also let the filament cool 5 to 10 minutes whenever possible before
venting. I don't have any hard evidence, but it just seems logical to me
that exposing a hot tungsten filament to atmosphere is going to induce
more oxidation or whatever compared to a cool one.

In my experience, these precautions contribute more to filament life
than slight undersaturation. Of course, you want to make sure you're not
oversaturated, but I get 3-400 hours of the Hitachi filaments running in
the "medium" gun setting.

Hope this helps.

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Simple, clear purpose and principles give
rise to complex and intelligent behavior.
Complex rules and regulations give rise
to simple and stupid behavior.
— Dee Hock


On 2/9/2018 5:09 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: wschneider-at-wisc.edu Name: Bil Schneider
} Organization: UW Madison
} Title-Subject: [Filtered] Tungsten filaments
}
} Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does
} anyone have any suggestions for extending filament life? We try and
} operate slightly under saturated except for EBSD or EDS mapping. IÂ’ve
} heard of “seasoning” the filament but have never seen an actual protocol
} or talked with anybody whoÂ’s actually done it. Any suggestions would be
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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 00:31:19 -0600
Subject: [Microscopy] viaWWW:Symposium P06, M&M2018

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Email: okneill-at-umich.edu Name: Owen Neill

Organization: University of Michigan

Title-Subject: [Filtered] Symposium P06, M&M2018

Message: Dear colleagues,

Apologies for the cross-postings. The deadline for abstract submission
for the Microscopy & Microanalysis 2018 Annual Meeting (Baltimore, MD,
5-9 August, 2018) is February 15th, just a week away. We hope you will
consider submitting an abstract to:

Symposium P06, Applications of Integrated Electron Probe Microscopy and
Microanalysis in Characterizing Natural and Synthetic Materials.

A full description of this session is included below – we are looking
for submissions dealing with the synthesis of different microbeam
techniques for evaluating composition, bonding, valence, and
density-of-state in natural and synthetic materials. Papers may be
submitted via the M&M2018 website, which is linked here:
https://www.microscopy.org/MandM/2018/index.cfm

We are also pleased to announce the invited speakers for this symposium:
Emma Bullock, Carnegie Institution
Paul Edwards, Strathclyde University
Raynald Gauvin, McGill University
Peter Statham, Oxford Instruments
Ed Vicenzi, Museum Conservation Institute

We are very excited to hear from these experts in microanalysis, and we
look forward to hearing about your work as well. See you in Baltimore!

The organizers of Symposium P06:
Kat Crispin, Pennsylvania State University
Colin MacRae, CSIRO Mineral Resources
Owen Neill, University of Michigan

Symposium Description:
Electron beam instruments have been merging with other techniques to
extract information such as bonding, valence, and density-of-state,
which, combined with elemental analysis, offers new insights into the
structure and chemistry of materials. Researchers now have access to an
analytical toolbox enabling characterization in a range of controlled
environments (liquid and gas) and conditions (elevated temperatures to
liquid helium). This symposium showcases synergies of new and emerging
techniques, while highlighting recent advances in areas such as optical
analysis using RAMAN, cathodoluminescence, soft X-ray spectrometry, and
others. Papers covering some or all of these new developments and
re-emerging technologies are welcome.

Topics of interest include:
• Multi-technique, electron-microbeam-based characterizations
of natural and synthetic materials.
• Integration of new and existing technologies to measure
material chemistry, structure, boding, valence, etc.
• Applications of such technologies to natural and synthetic
materials.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 00:31:56 -0600
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
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X-from: mike.marko.em-at-gmail.com

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Email: mike.marko.em-at-gmail.com Name: MIke Marko

Organization: NY

Title-Subject: [Filtered] Opportunity to learn Cryo-EM and get paid for it!

Message: Opportunity to learn Cryo-EM and get paid for it!

Dr. Rajendra Agrawal’s group at Wadsworth Center in Albany, NY is
looking for an electron microscopy technologist for cryo-EM data collection.

This is an opportunity for someone who knows the basics of TEM operation
to learn the “hottest technique in biology”. You will be using high-end
state-of-the-art equipment and software and will be part of an
internationally competitive group that has years of experience and
consistent grant funding. Our group evolved from the pioneering work --
at Wadsworth -- of Joachim Frank, who shared the 2017 Nobel prize.
Other PIs at our 3DEM facility are at the forefront of cryo-EM
development, so Albany can be an exciting place to be. Living expenses
in Albany are moderate, and our area is an attractive place to live.

If you are interested in this opportunity, please contact Dr. Agrawal at
rajendra.agrawal-at-health.ny.gov



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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 18:53:26 -0600
Subject: [Microscopy] viaWWW: nfo on Tungsten Filament optimization ,Message: Thanks to all

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Email: wfschneider-at-wisc.edu Name: Bil Schneider

Organization: UW Madison Geosciences

Title-Subject: [Filtered] Info on Tungsten Filament optimization

Message: Thanks to all who responded to my S3400N VPSEM tungsten
filament questions.

We do try to keep users from venting for 5 minutes after turning off the
HV. We probably average 50 hours per filament lately, which is what
aggravated me a bit because the last box was more like 80 hours. One
challenge is that some users change samples multiple times in a session.
Then the next user is in variable pressure changing mag and kV, vacuum,
etc..... then someone will run an EBSD map overnight at 30 kV, 100 probe
current. We ask a lot from the SEM, in general it’s a workhorse. I did
have several suggestions that seem promising:
The Hitachi guy called and suggested new filaments shouldn’t be turned
on till Vacuum has worked for a couple of hours. He said they try and
wait more like 15 minutes between HV off and venting.
Several responders further emphasized “gently” saturating the filament
over a few hours, keeping it slightly under saturated for general use,
and using some gun bias to concentrate the emission area. One respondent
made a great point about using a vacuum gauge on the gun and waiting
till Vacuum is in the 10 to the minus 5 range. I honestly don’t know
what the vacuum is when the Hitachi lets you turn on the HV.
Adding more spacers to push the grid cap further away from the filament
tip was another suggestion. I was under the impression that how ever
many spacers that each individual filament came with was the correct
distance as measured at the factory to keep emission current appropriate?
Otherwise I handle everything with gloves and care when changing
filaments and occasionally use metal polish on the grid cap and threaded
holder as well as the anode. More often I’ll sonicate the grid cap and
holder in methanol when the gun is open to change a filament. Thanks
again for all the suggestions, I’ll repost this in hopes this collection
of information might be useful to others with tungsten filament questions.
Cheers,
Bil Schneider UW Geosciences

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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 18:54:22 -0600
Subject: [Microscopy] viaWWW:Cross section on MgO substrates

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X-from: corinne.ulhaq-at-ipcms.unistra.fr

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Email: corinne.ulhaq-at-ipcms.unistra.fr Name: Crinne Bouillet

Organization: CNRS

Title-Subject: [Filtered] Cross section on MgO substrates

Message: Hello
I need to study the quality of interface of few nanometer thin layer on
MgO substrate. I 've prepared cross sections by mechanical polishing
followed by ion milling usig the PIPS. I am not satisfied by the quality
of HAADF STEM images, especially when I compared those images to HAADF
STEM images from cross sections on SrTiO3 prepared by the wedge
polishing with the multiprep from ALLIED. Did someone already succeed in
performing cross section on MgO using the wedge polishing? I 've tried
several time without succes. What is the angle used, the strength, the
trick?
Thank you for your help

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From: steven.spurgeon-at-pnnl.gov
Date: Mon, 12 Feb 2018 18:08:23 -0600
Subject: [Microscopy] Suggestions for vacuum desiccator sample containers?

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X-from: Tom Hrn {tomas.hrncir-at-tescan.com}
\
Hello All,
in my experience there are 3 very simple rules to extend the tungsten
filament lifetime:

1. Vent the microscope by nitrogen and always exchange the sample and
pump the chamber quickly. This will minimize the contamination of the
vacuum chamber by water and speed up the pumping.

2. After getting HV ready signal (when the gun/chamber is pumped), wait
several tens of seconds or longer before heating the filament - the
filament lifetime depends on the pressure exponentially. Reaching lower
10-3 Pa in the gun is perfect to extend the filament lifetime.

3. Turn off filament heating manually and wait several minutes before
venting the chamber. Like that, the filament will cool down properly.
Venting the filament when it is hot decreases its lifetime significantly.

By utilizing long waiting time, the filament lifetime around 1000 hours
can be obtained easily, if there is no vacuum leak in the gun. So
sometimes it is a question whether to wait longer and reach very long
lifetime, or wait shorter and exchange filaments more frequently
(changing tungsten filament is cheap and easy). For someone and on some
machines, exchanging the filament might take several minutes only so it
does not make sense to wait several minutes during each sample exchange
procedure. For other users/machines, exchanging the filament might be a
nightmare so it would be better to wait longer and extend the filament
lifetime :-).
Have a nice day,
Tomas

--
Tomas Hrncir, Ph.D.
Senior R&D Physicist

p: +420 530353468
a: TESCAN Brno, s.r.o. | Libuina tda 1, 623 00 Brno, Czech Republic
w: www.tescan.com | e: tomas.hrncir-at-tescan.com


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From rodehora0-at-gmail.com Mon Feb 12 17:17:56 2018
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Message-ID: {69997897.792C5529-at-gmail.com}

Hello everyone,

We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes.

Does anyone have recommendations for other containers we can consider? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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7, 38 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
7, 38 -- Subject: Suggestions for vacuum desiccator sample containers?
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Feb 2018 00:51:59 -0600
Subject: [Microscopy] viaWWW:Electron Microscopist position in Halle, Germany

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X-from: panagiotis.kastritis-at-embl.de

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Email: panagiotis.kastritis-at-embl.de Name: Dr Panagiotis Kastritis

Organization: European Molecular Biology Laboratory, Heidelberg
Title-Subject: [Filtered] Electron Microscopist position in Halle, Germany

Message: Dear EM community,

I would like to draw your attention to an open position of EM facility
manager at the HALOmem institute, located at the Martin Luther
University of Halle-Wittenberg (MLU) in Halle, Germany.
Our cryoEM facility includes the 300 kV JEOL JEM-3200FSC (equipped with
field emission gun, cryo-stage, in-column energy filter and GATAN K2
direct electron detector). Funds are available to acquire another
high-end cryo-electron microscope. We are searching for a staff
scientist/facility manager who will oversee the operation of the cryoEM
facility: assist users with training on the microscopes, perform data
collection and ensure timely maintenance of the microscopes. The
position is offered until August 2022 and the call closes on the 5th of
March. The candidate should have an extensive experience in electron
microscope operation, preferably under cryogenic conditions. The HALOmem
institute (http://www.halomem.de/) is interested in membrane protein
research and houses research groups with strong expertise in protein
biochemistry, X-ray crystallography, NMR and proteomics.
Interested candidates should apply via e-mail or request further
information from Dr. Panagiotis Kastritis (pk-at-halomem.de)
--
Official call (in german):
http://www.verwaltung.uni-halle.de/dezern3/Ausschr/18_138.pdf
English translation:
http://www.halomem.de/images/uploads/EM_manager_ad-eng_inofficial.pdf
Job details (in english):
http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf
(http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf)
---

Thank you very much, Kindest Regards,

Panos
--
Dr. Panagiotis Kastritis
Structural and Computational Biology Unit
European Molecular Biology Laboratory
Meyerhofstra?e 1, 69117 Heidelberg, Germany

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From: lists-at-nexperion.net
Date: Tue, 13 Feb 2018 02:41:03 -0600
Subject: [Microscopy] Re: Suggestions for vacuum desiccator sample containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steven,

} We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes.
} Does anyone have recommendations for other containers we can consider? Thanks!

I have been using this model for storage and transport of SEM samples but also whole TEM grid boxes:

http://scienceservices.de/en/sample-stub-vacuum-desiccator.html

They work really well, up to the point where they start questioning you at the airport security check where you'd be going with that "crystal ashtray" ;)

Guenter

--
Dr. Guenter Resch
Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net
Ziegelhofstrasse 136/1, 1220 Vienna, Austria - Phone +43 664 94 17 210
Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234


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From: diller-at-stefan-diller.com
Date: Tue, 13 Feb 2018 04:18:24 -0600
Subject: [Microscopy] Re: FW: Ask a Microscopist - Imaging board needed for a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Karl,

if your SEM computer has still control of vacuum / high tension and scan amp, the easiest solution would be to attach an external
scan system like the DISS5 from www.pointelectronic.de I am using.

Next possibility would be to exchange the complete electronics to a new one. Pointelectronic does this for some of the Zeiss / FEI
/ Jeol scopes. There should be an US-based agent also for their hardware.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 10.02.18 um 00:21 schrieb oshel1pe-at-cmich.edu:
} ----------------------------------------------------------------------------
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}
} Name:karl Hagglund
} School:Northwestern University
} Grade/Education Level:Graduate
} Location:Evanston, IL
} US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject}
} Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board
} Your Question:
} Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts.
} Thank you,
} Karl Hagglund
}
}
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Feb 2018 14:07:15 -0600
Subject: [Microscopy] Fwd: Tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
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X-from: Steve Chapman {protrain-at-emcourses.com}

Hi

I have just picked up this offering and with 50 years EM experience I
fully agree with Tomas' posting. This procedure should be in your
s.o.p. for running a scanning electron microscope, any other way is just
not good enough!

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com



-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com] Sent: 12 February 2018 21:15
To: protrain-at-emcourses.com

X-from: Tom Hrn {tomas.hrncir-at-tescan.com} \ Hello All, in my experience
there are 3 very simple rules to extend the tungsten filament lifetime:

1. Vent the microscope by nitrogen and always exchange the sample and pump
the chamber quickly. This will minimize the contamination of the vacuum
chamber by water and speed up the pumping.

2. After getting HV ready signal (when the gun/chamber is pumped), wait
several tens of seconds or longer before heating the filament - the filament
lifetime depends on the pressure exponentially. Reaching lower
10-3 Pa in the gun is perfect to extend the filament lifetime.

3. Turn off filament heating manually and wait several minutes before
venting the chamber. Like that, the filament will cool down properly.
Venting the filament when it is hot decreases its lifetime significantly.

By utilizing long waiting time, the filament lifetime around 1000 hours can
be obtained easily, if there is no vacuum leak in the gun. So sometimes it
is a question whether to wait longer and reach very long lifetime, or wait
shorter and exchange filaments more frequently (changing tungsten filament
is cheap and easy). For someone and on some machines, exchanging the
filament might take several minutes only so it does not make sense to wait
several minutes during each sample exchange procedure. For other
users/machines, exchanging the filament might be a nightmare so it would be
better to wait longer and extend the filament lifetime :-).
Have a nice day,
Tomas

--
Tomas Hrncir, Ph.D.
Senior R&D Physicist

p: +420 530353468
a: TESCAN Brno, s.r.o. | Libuina tda 1, 623 00 Brno, Czech Republic
w: www.tescan.com | e: tomas.hrncir-at-tescan.com

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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Feb 2018 15:49:37 -0600
Subject: [Microscopy] viaWWW:Career Opportunity - Electron Microscopy Technologist

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Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Career Opportunity - Electron Microscopy
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Message: Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated
individual to work in a state-of-the-art Microscopy Shared Resource.
The individual must have extensive practical expertise in biological
sample preparation for transmission and scanning electron microscopy of
animal tissues and mammalian cell lines. Hands-on knowledge of confocal
and widefield fluorescence microscopy would also be a plus.

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How to Apply

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Position Number 01779-R

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Cold Spring Harbor Laboratory is a world-renowned research and
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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Feb 2018 03:05:23 -0600
Subject: [Microscopy] viaWWW:GWNIC to host afternoon of high pressure freezing lectures

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] GWNIC to host afternoon of high pressure
freezing lectures

Message: Hi all,

The George Washington University Nanofabrication and Imaging Center
would like to invite you to an afternoon of high pressure freezing
lectures on Monday March 5, 2018. So if you are in Washington DC, just
drop around and spend an inspiring afternoon with some local experts in
the field of high pressure freezing.

https://nic.gwu.edu/high-pressure-freezing

Chris Brantner

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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Feb 2018 03:06:25 -0600
Subject: [Microscopy] viaWWW:old style FEI camera film cassette holder

Contents Retrieved from Microscopy Listserver Archives
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X-from: tina.williams-at-ars.usda.gov

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Email: tina.williams-at-ars.usda.gov
Name: Tina Williams

Organization: USDA

Title-Subject: [Filtered] old style FEI camera

Message: Hello,

We have an old style FEI camera film cassette holder that fits an FEI
Technai 12. If anyone is interested please contact me offline.

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From: frank_karl-at-ardl.com
Date: Wed, 14 Feb 2018 09:22:45 -0600
Subject: [Microscopy] TEM question answered

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I want to thank everyone who responded to my TEM question and I regret things are so crazy I can't respond to each person. The information provided was very useful and I plan to remove and clean the Wehneit cup and reinstall. That should, I hope resolve my problems.





Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Feb 2018 14:48:17 -0600
Subject: [Microscopy] viaWWW:Job Opening - NASA Johnson Space Center - SEM/EBSD

Contents Retrieved from Microscopy Listserver Archives
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X-from: Lindsay.P.Keller-at-nasa.gov

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Email: Lindsay.P.Keller-at-nasa.gov Name: Lindsay Keller

Organization: NASA

Title-Subject: [Filtered] Job Opening - NASA Johnson Space Center - SEM/EBSD

Message: Job opening at NASA JSC with Jacobs for a Planetary Sample
Scientist specializing in electron beam analyses of materials primarily
using SEM/EBSD techniques. position description and qualifications at:
https://jacobs.taleo.net/careersection/ex/jobdetail.ftl?job=AS0002SE&tz=GMT-06%3A00

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From: zaluzec-at-microscopy.com
Date: Wed, 14 Feb 2018 14:50:54 -0600
Subject: [Microscopy] viaWWW:JEM-2100 Plus Parts Need - Virtually Urgent

Contents Retrieved from Microscopy Listserver Archives
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X-from: rvancamp-at-kettering.edu

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Email: rvancamp-at-kettering.edu Name: Rick

Organization: Kettering University

Title-Subject: [Filtered] JEM-2100 Plus Parts Need - Virtually Urgent

Message: Hello Everyone,

I am the microscopist at Kettering University. We own a JEM-2100 Plus
TEM. I recently observed that the Wehnhelt and the cathode mounting
base the Wehnhelt screws onto have experienced damage to their mating
threads. This precludes these parts from being used. I am posting this
message to the community in an attempt to identify parts we can obtain
from those that have these two parts in addition to the ability to loan
them to us on a temporary basis or sell them to us.

The JEM-2100 Plus features a Wehnelt and cathode mount that are common
to many Jeol instruments yet, I do not have a complete list. We must
consider consulting Jeol to ensure compatibility before any transaction
occurs. Note this is the Jeol K-type cathode mount. For example, our
TEM uses the Jeol MA113008 (03) tungsten filament, the Denka M3 LKSH
60-degree sharp tip LaB6 cathode, and the APTech mini-Vogel mount 15927
LaB6 cathode. These cathodes are provided as a reference only. I do
not know the complete list of LaB6 cathodes that are compatible with our
JEM-2100 Plus.

Please contact me if you have additional questions or think you are in a
position to help us. Thank you for your consideration.

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From: dev.c0debabe-at-gmail.com
Date: Thu, 15 Feb 2018 09:40:41 -0600
Subject: [Microscopy] Norad Quest EDX - system voltage measurements

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,
I am teaching a TEM class and I am having trouble finding reliable, readable mappings of the Kikuchi lines for diamond Si. There seems to be a couple of hand-drawn ones floating around, but they are very low-res. I was wondering if anyone had one they could share, or direct me to a place where they can be simulated?

Thank you!

==============================Original Headers==============================
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From solizbula031yzos-at-gmail.com Thu Feb 15 01:29:34 2018
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Message-ID: {3B082089.20CA5429-at-gmail.com}

Hi,

I recently got a second hand Noran Quest EDX system which I currently
can't power on since the detector temperature is too high.
I let it cool down for 24 hours with a fully filled up dewar.
I believe that the measurement of the "detector temp" is wrong since
there is no voltage (0.00 Volts) measured at all. Also, there is no
voltage difference between a warm detector and a detector that has
cooled down for 24 hours. I am thus looking for technical information
including schematics. I suspect that there could be a contact issue or a
broken cable.

I started the Noran Quest "sys_status" tool. It gives me the following
output:

--------------------------------------------------------------
Detector Temp: 0.00 Volts LN Sensor: 4.28 Volts
Detector Bias: -5.66 Volts Reference: 1.60 Volts
Aux 1: 0.01 Volts Chassis Temp: 6.48 Volts
Aux 2: 0.02 Volts +5V: 4.91 Volts
+24V: 24.06 Volts -24V: -24.05 Volts
+15V: 14.57 Volts -15V: -14.84 Volts
+5V Analog: 4.85 Volts -5V Analog: -5.07 Volts
--------------------------------------------------------------

As you can see, the "detector temp" is 0.00 Volts.


- What are normal voltage levels for the temperature measurements ?
(If the LN sensor voltage is 4.28 Volts, how does that translate
to °C ?
Is there a formula to translate the voltage to temperature?
Is 4.28 Volts OK for a filled up dewar ?)

- What voltage should I get for the "detector temp" ?
(What voltage should be measured if the detector is warm ?
What voltage should be there if the detector is cooled down ?)

- Where is the temperature sensor physically located within the detector ?

- What kind of temperature sensor is it (resistor with X Ohms) ?

- At the detector there is a d-sub connector. What is the pinout of this
connector and where is the "detector temp" output ?


Any technical information to get my detector working would be really
appreciated.

Thank you,
Stefan




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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 03:41:38 -0600
Subject: [Microscopy] viaWWW: ancient grain imaging help needed

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Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan

Organization: The University of Adelaide, Adelaide Microscopy

Title-Subject: [Filtered] ancient grain

Message: Hi all,
I have been tasked with imaging an ancient grain. It is 1000 year old
millet and I have one only! I have done SEM/TEM of grain before but not
one so old. I am thinking SEM may be the way to go. The investigators
would like to see the internal structure of the grain (if any) and I
have no idea whether it will be 'normal', caramelised or powder inside!
It must be fixed in order to be released from quarantine so my first
question is should I use an aqueous fixative or alcohol?
Any other advice would be gratefully accepted!

Cheers
Lisa

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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 03:42:46 -0600
Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?

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Email: btracy-at-eag.com Name: Bryan Tracy

Organization: EAG Laboratories

Title-Subject: [Filtered] cutting the slab?

Message: Hello Everyone,

I know this is a subject on which reasonable people can disagree, but I
wanted to ask what has been your experience with cutting the slab to
reduce low frequency vibrations?

The floor appears to be moving in the horizontal plane at 1.6Hz (4.4
reduction factor needed) and in the vertical direction at 5HZ. (3.1
reduction factor needed)

much appreciated

bryan

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From: John.Mardinly-at-asu.edu
Date: Fri, 16 Feb 2018 11:24:20 -0600
Subject: [Microscopy] Re: viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Bryan;
3-4X attenuation of ambient vibrations is very difficult. Finding another location would be the preferred solution.

A. John Mardinly, Ph.D., P.E.
The Sleep of Reason Produces Monsters
Francisco Goya



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} Email: btracy-at-eag.com Name: Bryan Tracy
}
} Organization: EAG Laboratories
}
} Title-Subject: [Filtered] cutting the slab?
}
} Message: Hello Everyone,
}
} I know this is a subject on which reasonable people can disagree, but I
} wanted to ask what has been your experience with cutting the slab to
} reduce low frequency vibrations?
}
} The floor appears to be moving in the horizontal plane at 1.6Hz (4.4
} reduction factor needed) and in the vertical direction at 5HZ. (3.1
} reduction factor needed)
}
} much appreciated
}
} bryan
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 14:19:29 -0600
Subject: [Microscopy] viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Lisa,

It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
much anyway. If alcohol is good enough to release from quarantine, use
70 or 80% ethanol, then go through to 100% EtOH.
Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
at 20 deg C.

After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
get a brittle fracture that will expose the starch grains.
This part will be particularly fun (or “fun”) if your grain is tiny ...

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab





Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan

Organization: The University of Adelaide, Adelaide Microscopy

Title-Subject: [Filtered] ancient grain

Message: Hi all,
I have been tasked with imaging an ancient grain. It is 1000 year old
millet and I have one only! I have done SEM/TEM of grain before but not
one so old. I am thinking SEM may be the way to go. The investigators
would like to see the internal structure of the grain (if any) and I
have no idea whether it will be 'normal', caramelised or powder inside!
It must be fixed in order to be released from quarantine so my first
question is should I use an aqueous fixative or alcohol?
Any other advice would be gratefully accepted!

Cheers
Lisa



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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 14:20:40 -0600
Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Mike Toalson {miketoalson-at-gmail.com}


Hi Bryan,

Do you know if the vibration is resulting form sources inside the
building or the from the general area outside?  EAG is in between a
number of freeways there in Sunnyvale and the 101 isn't far away so if
it is the underlying ground below slab, you might make it worse cutting
the slab.  I'm sure some of the experts on here have experience doing
this and will advise.

You might also check with Vibration Engineering Consultants -
www.vibeng.com {http://www.vibeng.com} - in Pacifica, CA. This is their
specialty.

I would also be remiss if I didn't suggest that you consider a Vibration
Isolation Base Platform.  The active type we offer suppresses vibration
starting at 0.7 Hz and has 90% suppression by 2Hz. I would avoid a
passive type isolator as most of them have a resonant frequency right
around 2 to 4 Hz which would make things even worse for the vibrations
you have.  Likewise if you decide to cut the slab, careful if you use an
elastomeric sealant and check it's resonant frequency.

Have a look here at our line of Vibration Isolation Platforms for
SEM/TEM/FIB:
http://www.elementpi.com/active-vibration-isolation-platform-products/

Good Luck,

Mike Toalson
element Pi, LLC
833-314-1593


On Fri, Feb 16, 2018 at 2:15 AM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:





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Email: btracy-at-eag.com {mailto:btracy-at-eag.com} Name: Bryan Tracy

Organization: EAG Laboratories

Title-Subject: [Filtered] cutting the slab?

Message: Hello Everyone,

I know this is a subject on which reasonable people can disagree, but I
wanted to ask what has been your experience with cutting the slab to
reduce low frequency vibrations?

The floor appears to be moving in the horizontal plane at 1.6Hz  (4.4
reduction factor needed) and in the vertical direction at 5HZ.  (3.1
reduction factor needed)

much appreciated

bryan

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From: Rosemary.White-at-csiro.au
Date: Fri, 16 Feb 2018 19:05:22 -0600
Subject: [Microscopy] Re: viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

All,

See below for a request from a paleobiologist here at the National Museum of Natural History. If you have a modern image of collagen fibrils you are willing to share in some manner he would be interested in hearing from you directly. His contact is GreenwaltD-at-si.edu.

Thanks,

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891  mailto:whittaks-at-si.edu
 
SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
https://www.facebook.com/nmnh.fanpage/  |  https://twitter.com/NMNH  |  https://www.instagram.com/smithsoniannmnh/

X-from: "Greenwalt, Dale" {GreenwaltD-at-si.edu}

Hi Lisa,

Fixation in 95-100% methanol or ethanol may be better for your grain - there will be less tissue swelling. The dry grain will contain only about 8-10% water, so going into 100% solvent will be fine. A little water (i.e. 95% solvent) may help penetration. Methanol will penetrate a little better, but it will take some time for any solvent to get deep into a dry grain.

Another option after drying would be high-resolution (+/- phase contrast) x-ray CT - it would quickly show you if the grain had any contents, and give you an idea of what they are without breaking the seed. There are at least a couple of labs I know of in Australia that do this, one of them is here in Canberra at the ANU - https://ctlab.anu.edu.au/, and since your seed will be dead after going through solvent, you don't have to worry about the high kV x-rays killing the tissue. Millet seed is pretty small, so you'd get good resolution of the innards.

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
Canberra, ACT 2601
Australia
Adjunct Prof, EH Graham Centre, CSU
& Research School of Biology, ANU

T 61 2 6246 5475
E rosemary.white-at-csiro.au


On 17/2/18, 7:29 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:




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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Lisa,

It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
much anyway. If alcohol is good enough to release from quarantine, use
70 or 80% ethanol, then go through to 100% EtOH.
Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
at 20 deg C.

After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
get a brittle fracture that will expose the starch grains.
This part will be particularly fun (or “fun”) if your grain is tiny ...

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab





Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan

Organization: The University of Adelaide, Adelaide Microscopy

Title-Subject: [Filtered] ancient grain

Message: Hi all,
I have been tasked with imaging an ancient grain. It is 1000 year old
millet and I have one only! I have done SEM/TEM of grain before but not
one so old. I am thinking SEM may be the way to go. The investigators
would like to see the internal structure of the grain (if any) and I
have no idea whether it will be 'normal', caramelised or powder inside!
It must be fixed in order to be released from quarantine so my first
question is should I use an aqueous fixative or alcohol?
Any other advice would be gratefully accepted!

Cheers
Lisa



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From: microscopy.listserver-at-gmail.com
Date: Sun, 18 Feb 2018 10:52:46 -0600
Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Henk Colijn {colijn.1-at-osu.edu}
Reply-To: Henk Colijn {colijn.1-at-osu.edu}
To: microscopy.listserver-at-gmail.com

Hi Bryan,

Those are very low frequencies. They should be in a range that an active compensation system should
be able to handle it.

Another thing to consider is the resonant frequency of the slab (even though it is damped by the
ground). The more mass in the slab, the lower its resonant frequency. The install guides for our
microscopes show that the microscope is much more sensitive at very low frequencies. The allowable
vibration at 10Hz is approx 10x lower than at 20Hz. Since too much mass can push the slab resonance
down into the range where the microscope is more sensitive you may want to estimate the resonant
frequency of your current slab and then consider whether to slice it or not. It would also be
useful to measure the ground vibration away from the instrument to get an idea of the driving
frequencies.

Good luck!
Henk



--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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To: colijn.1-at-osu.edu
Sent: 2/16/2018 4:45:19 AM


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From: microscopy.listserver-at-gmail.com
Date: Sun, 18 Feb 2018 10:53:17 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
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X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}



Thanks Phil.
I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and
then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking
this grain might have a more detrimental effect.
Thanks for your advice

Cheers
Lisa

Dr Lisa O'Donovan

Microscopist

Adelaide Microscopy

Division of Research and Innovation

The University of Adelaide,

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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:

}
}
}
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} X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
}
} Lisa,
}
} It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
} much anyway. If alcohol is good enough to release from quarantine, use
} 70 or 80% ethanol, then go through to 100% EtOH.
} Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
} at 20 deg C.
}
} After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
} nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
} get a brittle fracture that will expose the starch grains.
} This part will be particularly fun (or “fun”) if your grain is tiny ...
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
} 989 774-7567 lab
}
}
}
}
}
} Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan
}
} Organization: The University of Adelaide, Adelaide Microscopy
}
} Title-Subject: [Filtered] ancient grain
}
} Message: Hi all,
} I have been tasked with imaging an ancient grain.  It is 1000 year old
} millet and I have one only! I have done SEM/TEM of grain before but not
} one so old.  I am thinking SEM may be the way to go.  The investigators
} would like to see the internal structure of the grain (if any) and I
} have no idea whether it will be 'normal', caramelised or powder inside!
} It must be fixed in order to be released from quarantine so my first
} question is should I use an aqueous fixative or alcohol?
} Any other advice would be gratefully accepted!
}
} Cheers
} Lisa
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 08:28:01 -0600
Subject: [Microscopy] Fwd: Re: Fwd: Critical point dryer suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Factor, Jan {Jan.Factor-at-purchase.edu}



Dear List:

I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
faculty and undergraduate student users for research projects, and trains undergraduates via
courses. After training, users carry out their own specimen preps.

Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.

I would appreciate any comments from folks who have experience with either of these, as well as
suggestions about other models I may have overlooked but should consider.

Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
{mailto:jan.factor-at-purchase.edu} .

--Many thanks, *Jan *

Jan Robert Factor, Ph.D.

Professor of Biology

Purchase College, State University of New York

Purchase, NY 10577

Office: 2016 Natural Science Bldg., 914-251-6659

Office Hours (Spring 2018):

Tues., 11:00-12:00, and Thurs., 1:30-2:30

jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}

/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
{http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
*Please consider the environment before printing this e-mail*


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From donnedua0-at-gmail.com Mon Feb 19 03:22:36 2018
Return-Path: {donnedua0-at-gmail.com}
Received: from gmail.com (hn.kd.ny.adsl [222.139.205.246] (may be forged))
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To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

We have the Tousimis 815B & 915B. We process a lot of samples of varying sizes (some quite large) &
they do an excellent job & are easy/straightforward to use. We have an advantage since we're pretty
much next door to the company, but they are very helpful. I haven't used the EMS.

Deborah

Sent from my iPhone

} On Feb 18, 2018, at 11:16 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
}
}
}
}
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} X-from: Factor, Jan {Jan.Factor-at-purchase.edu}
}
}
}
} Dear List:
}
} I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
} faculty and undergraduate student users for research projects, and trains undergraduates via
} courses. After training, users carry out their own specimen preps.
}
} Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
}
} I would appreciate any comments from folks who have experience with either of these, as well as
} suggestions about other models I may have overlooked but should consider.
}
} Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
} {mailto:jan.factor-at-purchase.edu} .
}
} --Many thanks, *Jan *
}
} Jan Robert Factor, Ph.D.
}
} Professor of Biology
}
} Purchase College, State University of New York
}
} Purchase, NY 10577
}
} Office: 2016 Natural Science Bldg., 914-251-6659
}
} Office Hours (Spring 2018):
}
} Tues., 11:00-12:00, and Thurs., 1:30-2:30
}
} jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}
}
} /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
} {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
} *Please consider the environment before printing this e-mail*
}
}
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From: microscopy.listserver-at-gmail.com
Date: Sunday, 18February, 2018 at 12:29 To: Philip Oshel
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Lisa,

Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve
used it for corn and barley, but if you do have millet … oof.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








-----Original Message-----
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Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}

X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}



Thanks Phil.
I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and
then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking
this grain might have a more detrimental effect.
Thanks for your advice

Cheers
Lisa

Dr Lisa O'Donovan

Microscopist

Adelaide Microscopy

Division of Research and Innovation

The University of Adelaide,

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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:

}
}
}
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} X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
}
} Lisa,
}
} It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
} much anyway. If alcohol is good enough to release from quarantine, use
} 70 or 80% ethanol, then go through to 100% EtOH.
} Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
} at 20 deg C.
}
} After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
} nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
} get a brittle fracture that will expose the starch grains.
} This part will be particularly fun (or “fun”) if your grain is tiny ...
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
} 989 774-7567 lab
}
}
}
}
}
} Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan
}
} Organization: The University of Adelaide, Adelaide Microscopy
}
} Title-Subject: [Filtered] ancient grain
}
} Message: Hi all,
} I have been tasked with imaging an ancient grain. It is 1000 year old
} millet and I have one only! I have done SEM/TEM of grain before but not
} one so old. I am thinking SEM may be the way to go. The investigators
} would like to see the internal structure of the grain (if any) and I
} have no idea whether it will be 'normal', caramelised or powder inside!
} It must be fixed in order to be released from quarantine so my first
} question is should I use an aqueous fixative or alcohol?
} Any other advice would be gratefully accepted!
}
} Cheers
} Lisa
}
}
}
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From: gilpin-at-purdue.edu
Date: Mon, 19 Feb 2018 16:15:22 -0600
Subject: [Microscopy] evaporating Tin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Jan,

Tousimis is good, I’ve used those a lot. Not the Autosamdri, but the previous model. Have you
considered the Polaron “bomb”? The larger sample chamber (in the regular size) is very handy. SPI
sells this as a rebranded product. I use it in a multi-user facility that teaches undergrads and
grad students.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








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Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}

X-from: Factor, Jan {Jan.Factor-at-purchase.edu}



Dear List:

I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
faculty and undergraduate student users for research projects, and trains undergraduates via
courses. After training, users carry out their own specimen preps.

Two models I’ve come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.

I would appreciate any comments from folks who have experience with either of these, as well as
suggestions about other models I may have overlooked but should consider.

Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
{mailto:jan.factor-at-purchase.edu} .

--Many thanks, *Jan *

Jan Robert Factor, Ph.D.

Professor of Biology

Purchase College, State University of New York

Purchase, NY 10577

Office: 2016 Natural Science Bldg., 914-251-6659

Office Hours (Spring 2018):

Tues., 11:00-12:00, and Thurs., 1:30-2:30

jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}

/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
{http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
*Please consider the environment before printing this e-mail*

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From trojlita384otoj-at-gmail.com Mon Feb 19 10:46:40 2018
Return-Path: {trojlita384otoj-at-gmail.com}
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Hi Everyone,
I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.

We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Campus-wide Coordinator for Electron Microscopy
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx





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From: colijn.1-at-osu.edu
Date: Mon, 19 Feb 2018 17:10:42 -0600
Subject: [Microscopy] evaporating Tin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chris,

As the evaporated films get thicker, internal stress becomes
significant. I'm not sure how bad tin films are but I've had issues
with the films peeling when they got much over 0.1um thickness.

Good luck,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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To: colijn.1-at-osu.edu
Sent: 2/19/2018 5:17:35 PM



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From: wesaia-at-iastate.edu
Date: Mon, 19 Feb 2018 20:25:11 -0600
Subject: [Microscopy] evaporating Tin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Considering that is more than 100 times the thickness you normally put down, that is an issue.

I tried using evaporation to put down a thick layer of antimony on glass before. First, when I calculated the mass of Sb to put down a micron at a certain distance, I found I had to fill my tungsten basket to the full and maybe even run a couple of times. Then I think I found the thermal stresses Henk described. The layer curled up off the glass.

Why do they want such a thickness?

Warren

-----Original Message-----
X-from: gilpin-at-purdue.edu [mailto:gilpin-at-purdue.edu]
Sent: Monday, February 19, 2018 4:17 PM
To: Straszheim, Warren E [BIOTC]

Hi Everyone,
I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.

We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx





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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:54:05 -0600
Subject: [Microscopy] viaWWW:GWNIC EM position posted

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X-from: chrisbrantner-at-gwu.edu


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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: The George Washington University

Title-Subject: [Filtered] GWNIC EM position posted

Message: Greetings fellow microscopists,

I want to draw your attention to a position that I have open in my lab. If you are interested,
please follow the links.

https://www.gwu.jobs/postings/49625

https://www.gwu.jobs/postings/49512

Chris Brantner
Senior Research Scientist, Electron Microscopy
George Washington University Nanofabrication and Imaging Center



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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:55:27 -0600
Subject: [Microscopy] Fwd: Re: Critical point dryer suggestions

Contents Retrieved from Microscopy Listserver Archives
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X-from: Matt Russell {Matt.Russell-at-crick.ac.uk}



Hi Jan,

We have a CPD300 here that users have access to. One of our users is only trained on our bench top
SEM and she uses it regularly, independently.

Top tips for things that might catch out students;

1. As per the manual, the fillers should be on top of the holders. If you have them the other way
round the chamber doesn’t fill up correctly
2. Don’t overfill the chamber. Should be OK as long as the level is below the outlet hole at the
top, otherwise you might have some liquid left at the end.

It’s easy to use with a simple user interface; it’s been easy to train people on it. We use it quite
a lot and it gives nice results.

Cheers,

Matt

Matt Russell, PhD
Senior Laboratory Research Scientist
Electron Microscopy STP
The Francis Crick Institute
1 Midland Road
London
NW1 1AT

*T:* +442037962006
*E:* matt.russell-at-crick.ac.uk {mailto:matt.russell-at-crick.ac.uk}
*W:* www.crick.ac.uk {http://www.crick.ac.uk}

} On 18 Feb 2018, at 17:36, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
} wrote:
}
}
}
}
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} X-from: Factor, Jan {Jan.Factor-at-purchase.edu {mailto:Jan.Factor-at-purchase.edu} }
}
}
}
} Dear List:
}
} I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
} faculty and undergraduate student users for research projects, and trains undergraduates via
} courses. After training, users carry out their own specimen preps.
}
} Two models I抳e come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
}
} I would appreciate any comments from folks who have experience with either of these, as well as
} suggestions about other models I may have overlooked but should consider.
}
} Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
} {mailto:jan.factor-at-purchase.edu}
} {mailto:jan.factor-at-purchase.edu} .
}
} --Many thanks, *Jan *
}
} Jan Robert Factor, Ph.D.
}
} Professor of Biology
}
} Purchase College, State University of New York
}
} Purchase, NY 10577
}
} Office: 2016 Natural Science Bldg., 914-251-6659
}
} Office Hours (Spring 2018):
}
}  牋牋燭ues., 11:00-12:00, and Thurs., 1:30-2:30
}
} jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} {mailto:jan.factor-at-purchase.edu}
}
} /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
} {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
} *Please consider the environment before printing this e-mail*
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:56:47 -0600
Subject: [Microscopy] Fwd: Re: Fwd: viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Torraca, Gianni {gtorraca-at-amgen.com}

Cutting the slab is dangerous can may compromise the building. It is better to install active
vibration isolation. I have a number of SEMs in Thousand Oaks, CA. One LaB6 system is on the 2nd
floor. We use Herzan to reach vendor spec resolution with gold on carbon.
Regards
Gianni
________________________________________________________________________________________________________________________________
Gianpiero Torraca | Sr. Scientist | ATO Forensics | One Amgen Center Drive | Thousand Oaks, CA 91320
| Mailstop B30E-2B | 805.447.7445
“Mistakes are a fact of life. It is the response to the error that counts.” -Nikki Giovanni




-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday,
February 16, 2018 12:46 PM
To: Torraca, Gianni {gtorraca-at-amgen.com}

X-from: Darrell Miles {milesd-at-us.ibm.com}



One thing I don't think has been mentioned, is that the soil under many slab floors has settled, and
the slab is "floating" above without being supported. I have heard of holes being drilled and a
foam material being injected under the slab to support and dampen vibrations. This is done
sometimes, in addition to other vibration isolation measures.

Regards,
Darrell



microscopy.listserver-at-gmail.com wrote on 02/18/2018 11:56:14 AM:

} From: microscopy.listserver-at-gmail.com
} To: milesd-at-us.ibm.com
} Date: 02/18/2018 10:52 AM
} Subject: [Microscopy] Fwd: viaWWW: Vibrations: cutting the slab?
}
}
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} X-from: Henk Colijn {colijn.1-at-osu.edu}
} Reply-To: Henk Colijn {colijn.1-at-osu.edu}
} To: microscopy.listserver-at-gmail.com
}
} Hi Bryan,
}
} Those are very low frequencies. They should be in a range that an
} active compensation system should
} be able to handle it.
}
} Another thing to consider is the resonant frequency of the slab
} (even though it is damped by the
} ground). The more mass in the slab, the lower its resonant
} frequency. The install guides for our
} microscopes show that the microscope is much more sensitive at very
} low frequencies. The allowable
} vibration at 10Hz is approx 10x lower than at 20Hz. Since too much
} mass can push the slab resonance
} down into the range where the microscope is more sensitive you may
} want to estimate the resonant
} frequency of your current slab and then consider whether to slice it
} or not. It would also be
} useful to measure the ground vibration away from the instrument to
} get an idea of the driving
} frequencies.
}
} Good luck!
} Henk
}
}
}
} --------------------
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
}
} colijn.1-at-osu.edu 614/643-3458
} cemas.osu.edu
}
} "Time is that quality of nature which keeps things from happening
} all at once." (Ray Cummings - 1922)
} Lately it doesn't seem to be working.
}
} ------ Original Message ------
} X-from: microscopy.listserver-at-gmail.com
} To: colijn.1-at-osu.edu
} Sent: 2/16/2018 4:45:19 AM
} Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?
}
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} } Email: btracy-at-eag.com Name: Bryan Tracy
} }
} } Organization: EAG Laboratories
} }
} } Title-Subject: [Filtered] cutting the slab?
} }
} } Message: Hello Everyone,
} }
} } I know this is a subject on which reasonable people can disagree, but I
} } wanted to ask what has been your experience with cutting the slab to
} } reduce low frequency vibrations?
} }
} } The floor appears to be moving in the horizontal plane at 1.6Hz (4.4
} } reduction factor needed) and in the vertical direction at 5HZ. (3.1
} } reduction factor needed)
} }
} } much appreciated
} }
} } bryan
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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Feb 2018 08:52:54 -0600
Subject: [Microscopy] viaWWW:BIO TEM workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


X-from: LNMA CMN Siglo XXI {lnma.cmn.sigloxxi-at-gmail.com}



Hi, Lisa.
I am not an expert, but I would choose an analysis method knowing whether I will need to keep the
seed, or it can be used entirely.
Maybe I would try splitting it in parts (yep, it's millet, but still..) and then try different
several fixation methods.
I would also probably opt for hydration of the seed before fixation, but on an EtOH — to prevent
germination[?] or fungal growth vapor bath.
Interesting task!

Cheers,
Vad

On Mon, Feb 19, 2018 at 9:03 AM, {microscopy.listserver-at-gmail.com
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
To: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} }

Lisa,

Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve
used it for corn and barley, but if you do have millet … oof.

Phil
------------- Philip Oshel     Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








-----Original Message-----
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Date: Sunday,  18February, 2018 at 12:29 To: Philip Oshel {oshel1pe-at-cmich.edu
{mailto:oshel1pe-at-cmich.edu} }
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed




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X-from:         Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au
{mailto:lisa.odonovan-at-adelaide.edu.au} }



Thanks Phil.
I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and
then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking
this grain might have a more detrimental effect.
Thanks for your advice

Cheers
Lisa

Dr Lisa O'Donovan

Microscopist

Adelaide Microscopy

Division of Research and Innovation

The University of Adelaide,

ADELAIDE SA 5005

T: +61 8 8313 4074|

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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com}
{mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } "
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
{mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } wrote:

}
}
}
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} X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu}
{mailto:oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } }
}
} Lisa,
}
} It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
} much anyway. If alcohol is good enough to release from quarantine, use
} 70 or 80% ethanol, then go through to 100% EtOH.
} Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
} at 20 deg C.
}
} After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
} nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
} get a brittle fracture that will expose the starch grains.
} This part will be particularly fun (or “fun”) if your grain is tiny ...
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
} 989 774-7567 lab
}
}
}
}
}
} Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au}
{mailto:lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} } Name: Lisa O'Donovan
}
} Organization: The University of Adelaide, Adelaide Microscopy
}
} Title-Subject: [Filtered] ancient grain
}
} Message: Hi all,
} I have been tasked with imaging an ancient grain.  It is 1000 year old
} millet and I have one only! I have done SEM/TEM of grain before but not
} one so old.  I am thinking SEM may be the way to go.  The investigators
} would like to see the internal structure of the grain (if any) and I
} have no idea whether it will be 'normal', caramelised or powder inside!
} It must be fixed in order to be released from quarantine so my first
} question is should I use an aqueous fixative or alcohol?
} Any other advice would be gratefully accepted!
}
} Cheers
} Lisa
}
}
}
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--

Laboratorio Nacional de Microscopía Avanzada
Centro Médico Nacional Siglo XXI
Instituto Mexicano del Seguro Social
Av. Cuauhtémoc, No. 330, Col. Doctores
Mexico DF, México, 06720
Ubicación: https://goo.gl/3dsP0b
Tel: +52 (55) 5627 6900 * 20926, 20925
facebook.com/LMNA.CMN.SigloXXI {http://facebook.com/LMNA.CMN.SigloXXI}

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From michhyma42-at-gmail.com Wed Feb 21 05:24:39 2018
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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] Two seats left! - BIO TEM workshop

Message: Biological TEM Workshop

This intensive, three-day workshop provides a practical and basic theoretical introduction to the
Transmission Electron Microscope and biological sample preparation techniques. Each day consists of
lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday through Wednesday, March 14-16, 2018, 8am-5pm each day (lunch is provided)

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
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From: nathaniel.rieders-at-montana.edu
Date: Fri, 23 Feb 2018 13:37:09 -0600
Subject: [Microscopy] SEM Resolution Standard using FRC Method

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Hello, I am wondering if anybody can reccomend a standard/sample for determining microscope resolution in a SEM using the Fourier Ring Correlation (Autocorrelation) method. It is my understanding that suitable samples for TEM resolution estimates using FRC will not work in an SEM due to the differences in contrast mechanisms. Ideally, these would be samples which exhibit spatial frequencies from DC up to and beyond the resolution limit of the microscope.

Thank You,
Nathaniel Rieders

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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Feb 2018 18:48:16 -0600
Subject: [Microscopy] viaWWW:Zeiss Libra

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Email: rabara-at-tescan-usa.com Name: Michal Rabara

Organization: TESCAN USA, Inc.

Title-Subject: [Filtered] Microscopy Position Posted

Message: Organization: Tescan USA, Inc.

Email response: rabara-at-tescan-usa.com
Michal Rabara, CEO & President Tescan USA, Inc.

I would like to draw your attention to the Applications Manager position that we have open in North
America for Tescan USA, Inc. If you are interested, please follow the link below:

https://www.linkedin.com/jobs/view/602245101/


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From donnedua0-at-gmail.com Mon Feb 26 10:34:30 2018
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Title-Subject: [Filtered] Zeiss Libra

Message: Does anyone know of a qualified ISO that can support this TEM - Zeiss Libra 120 Plus TEM?

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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Feb 2018 18:49:22 -0600
Subject: [Microscopy] viaWWW:Applications Scientist TEM Semiconductors at Thermo Fisher

Contents Retrieved from Microscopy Listserver Archives
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Email: sorin.lazar-at-fei.com Name: Sorin Lazar

Organization: Thermo Fisher Scientific

Title-Subject: [Filtered] Applications Scientist TEM Semiconductors at Thermo Fisher Scientific,
Eindhoven

Message: Dear TEM community,

I would like to draw your attention to an Applications Scientist TEM Semiconductors open position at
Thermo Fisher Scientific in the Eindhoven Nanoport.

For those interested please follow the link below:

http://jobs.thermofisher.com/ShowJob/Id/156548/Applications-Scientist-TEM-Semiconductors/


Thanks and regards,

Sorin Lazar



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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Feb 2018 07:56:07 -0600
Subject: [Microscopy] viaWWW:Job Opening Applications Scientist TEM Materials Science at

Contents Retrieved from Microscopy Listserver Archives
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X-from: leonarddn-at-ornl.gov

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Email: leonarddn-at-ornl.gov Name: Donovan Leonard

Organization: ORNL

Title-Subject: [Filtered] Xe Plasma FIB consumables?

Message: Dear Xe Plasma FIB/SEM owners,

I am interested in learning what the potential cost of ownership of a Xe PFIB can be. In addition
to the service contract, what costs associated with apertures and sources are there? I am assuming
apertures have to be changed more frequently since beam currents can be on the order of microamps
(depending on hours in use). What about the Xe gas? I think this is just a small lecture bottle of
gas, but how often do you have to replenish that source?

Please respond off list to leonarddn-at-ornl.gov and thank you in advance. If you want to include any
anecdotes about Xe PFIB ownership I am happy to learn about that too (e.g. nightmares or winning
scenarios).

Donovan

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From fernayes9wybar-at-gmail.com Wed Feb 28 00:44:09 2018
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Email: sorin.lazar-at-fei.com Name: Sorin Lazar

Organization: Thermo Fisher Scientific

Title-Subject: [Filtered] job opening

Message:

"Dear TEM community,

I would like to draw your attention to an Applications Scientist TEM Materials Science open position
at Thermo Fisher Scientific in the Eindhoven Nanoport.

For those interested please follow the link below:
http://jobs.thermofisher.com/ShowJob/Id/156547/Applications-Scientist-TEM-Materials-Science/

Thanks and regards,
Sorin Lazar
"

With many thanks in advance,
Sorin





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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Feb 2018 13:34:42 -0600
Subject: [Microscopy] Fwd: vacuum system on a FEI Morgagni TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Pedro Leão {pedroleao-at-micro.ufrj.br}



Hi group.

I just change the filament on a FEI Morgagni TEM.
As I put the filament on the microscope and close the collum the PVP
does not start.

I reset the system, open the column again, I have no idea of what to do
next. Have anyone already had this problem? Any tips?

Thank you for your attention.

Best,

Pedro Leão

--
---

Instituto de Microbiologia - UFRJ
Laboratório de Biologia Celular e Magnetotaxia
CCS - Centro de Ciências da Saúde - Bloco I
Avenida Carlos Chagas Filho, 373
Cidade Universitária
CEP. 21941-902
Rio de Janeiro - RJ - Brasil
CV Lattes: http://lattes.cnpq.br/9781167318809464
LinkedIn:https://br.linkedin.com/in/pedro-leão-55992853
{https://br.linkedin.com/in/pedro-le%C3%A3o-55992853}
Tel. +55 21 3938 6738
Cel. +55 21 98131 3360




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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Feb 2018 19:44:15 -0600
Subject: [Microscopy] viaWWW:ORNL Seeks Postdoc, Helium Ion Microscopy

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X-from: millerme-at-ornl.gov

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Postdoc, Helium Ion Microscopy

Message: Postdoctoral Research Associate in Helium Ion Microscopy

Oak Ridge National Laboratory (ORNL) is looking for a Postdoctoral Research Associate to conduct
Helium Ion Microscopy (HIM) research and Secondary Ion Mass Spectrometry (SIMS) of a broad range of
inorganic and soft materials. Specifically, your research in this role will focus on imaging of soft
materials. For this role, experience in mass spectrometry (MS) and helium ion microscopy (HIM)
techniques, and/or other closely related areas is ideal. You will work with scientists and users at
the CNMS in developing new HIM capabilities in chemical imaging using SIMS, multimodal data
analysis, and integration with high performance computing environments. You will benefit from
experience in the analysis and characterization of soft materials and inorganic materials.

For more details, including the qualifications for this position, visit: http://bit.ly/PostdocHIM

ORNL is an equal opportunity employer. All qualified applicants, including individuals with
disabilities and protected veterans, are encouraged to apply.

Login Host: 128.219.49.14
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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Mar 2018 08:47:54 -0600
Subject: [Microscopy] Fwd: Save the date! North Atlantic Microscopy Society (NAMS) Nov. 1,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Gary Laevsky {glaevsky.lists-at-gmail.com}


Hi All!

We are very proud and excited to announce the formation of a new society, The North Atlantic
Microscopy Society (NAMS). Geographically centered at Princeton, NJ, we envision our coverage to
span southern New York, New Jersey and into Pennsylvania.


Edwin Hubble famously said, “Equipped with his five senses, man explores the universe around him and
calls the adventure Science.” We believe that some of this exploratory instinct has been muted
lately by our disciplinary silos. Individually, we have become exceptional in our specialties and do
not take moments to appreciate the many discoveries happening across the entire spectrum of science.

Our mission is to bridge these silos through the lens of microscopy. We seek to achieve this mission
by promoting microscopy education, stimulating networking among microscopists, and
disseminating microscopy knowledge and skills to the public in the region.


Our first Symposium will be held at Princeton University on November 1, 2018.

Our first speakers will be;

Hari Shroff (Light) - Chief of NIBIB's Section on High Resolution Optical Imaging laboratory.
Nieng Yan (Cryo-EM) - Shilrley M. Tilghman Professor of Molecular Biology at Princeton University.


This is exciting!!!

Gary Laevsky (Light Imaging)
Paul Shao (EM Imaging)
Tharan Srikumar (Mass Spec Imaging)


--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

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From: ALawrence-at-i2at.msstate.edu
Date: Fri, 2 Mar 2018 07:52:41 -0600
Subject: [Microscopy] Student help at the M&M 2018 meetings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Greetings
The deadline for paper submission has passed and meeting registration is now open. As you are making plans to attend the Baltimore, MD meetings (Aug. 5-9) please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.

The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events (paid by check at the end of the meetings). The jobs involve such things as providing support in the different symposia, staffing the volunteer office, newsletter distribution, and helping with vendor tutorial sign-up or in the outreach booth.

Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities they would like to help with. There is an additional bonus of $10 cash for each morning and/or afternoon session worked to assist with meals, along with a meeting t-shirt.

If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" on the registration form. Applicants for the bursaries must be a member of MSA or MAS and enrolled as a student at a recognized educational institution. Participants are responsible for their own registration fee and travel expenses.

For those of you who may not be students, but would still like to assist with the meetings, volunteers are also needed to fill the above-mentioned jobs. Although not paid the $10/hour as the students, volunteers do get a meeting t-shirt and the $10 cash for each morning/afternoon worked to help with meals.

If anyone has any questions about the bursary/volunteer program, or would like to participate, contact Amanda Lawrence: mailto:aml1819454-at-gmail.com (please do not reply to the email associated with this post). Bursary space is limited, so sign-up early.

See everyone in Baltimore,

Amanda Lawrence
Student Bursary/Volunteer Coordinator
Microscopy Society of America
mailto:aml181954-at-gmail.com



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From: vray-at-partbeamsystech.com
Date: Fri, 2 Mar 2018 15:30:00 -0600
Subject: [Microscopy] PSF extraction and convolution/deconvolution software or plug-in

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Dear Listers,

I am hoping someone could recommend somewhat-reliable software or (open source plug-in preferred) for extracting (approximated) 2D PSF from a pair of images of the same object, taken with closely matched contrast and same digital pixel count, but different beam current/diameter/profile. One of the images would be significantly sharper and serve as a reference, while the other "blurry" one is assumed to be a convolution of the reference image and PSF of the beam/instrument. The interest would be in extracting and examining PSF from the pair of images, convoluting extracted PSF with sharp image to estimate blurred one, and de-convoluting PSF from the blurred image to reconstruct sharp version. I am finding multiple plugins, but don't seem to come across something that would be convenient for all three operations.

Thank you very much beforehand,

Valery Ray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

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From: steven.spurgeon-at-pnnl.gov
Date: Fri, 2 Mar 2018 17:48:49 -0600
Subject: [Microscopy] Opening for SEM technologist and technician at PNNL

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Hello everyone,

I wanted to let you know that we’ve got two openings here at PNNL for an SEM technician and technologist to support imaging and sample preparation of nuclear materials. Candidates with a background in materials science and electron microscopy would be ideal.

Please feel free to forward the following links to anyone you think might be interested and don’t hesitate to contact me with any questions. Thank you.

https://pnnl.jobs/richland-wa/senior-technologist-nuclear-chemistry-engineering/2574E37174FD4D788A76BB296C193A15/job/

https://pnnl.jobs/richland-wa/senior-technician-nuclear-chemistry-engineering/1E805E40F8F245CDAD57E49E1B4D0DD7/job/

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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From: microscopy.listserver-at-gmail.com
Date: Mon, 5 Mar 2018 08:55:38 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW:PhD_scholarship_in_In-situ_TEM_and_Applications_of?=

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Technical Associate Staff Member - Scanning Probe Microscopy

Message: Technical Associate Staff Member - Scanning Probe Microscopy

Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff Member to support scanning
probe microscopy efforts at the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National
Laboratory (ORNL). In this role, you will be part of the Scanning Probe Microscopy Group, which is a
leader in the development and application of scanning probe microscopies and spectroscopies to image
and manipulate materials functionality in complex materials, ionic and electronic conductors,
molecular assemblies, and nanoscale structures. A suite of 18 commercial and internally developed
atomic force microscopes in ambient and controlled environments and scanning tunneling microscopes
in ultrahigh vacuum systems are used for internal and user science, with emerging opportunities in
enhanced data analytics and multimodal/chemical imaging.

For more details, including the qualifications for this position, visit: http://bit.ly/TP-SPM
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave

Organization: DTU Nanotech

Title-Subject: [Filtered] PhD scholarship in In-situ TEM and Applications of Nanowire Vapor Phase
Epitaxy Microsystems (VPE)

Message: We have a new PhD position open and I hope you will announce it on your website to help
recruiting!

PhD scholarship in In situ TEM and Applications of Nanowire Vapor Phase Epitaxy Microsystems (VPE)

please apply online via
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In this PhD project you will develop, fabricate and use microfabricated heater systems to study the
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imaging the process live!

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From: microscopy.listserver-at-gmail.com
Date: Mon, 5 Mar 2018 19:49:12 -0600
Subject: [Microscopy] viaWWW:thermal treatment of carbon-formvar film

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Email: dlowry-at-asu.edu Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] thermal treatment of carbon-formvar film

Message: a researcher here wants to do some thermal treatments (up to 150C) of samples after they
have been adhered to carbon-formvar coated silicon wafers (approx 13mm x 36mm) containing small
holes, and inquired with me about the max temp these films can endure without failing. She has
tested a blank film at her max temp and the film appeared to remain intact during heating, but fell
apart when removed from oven and during cool down. We have searched for published info on thermal
resistance of carbon-formvar but could not locate anything specific, so we are turning to the list
for advice. The films are home-made and approx 25-30 nm thick for the formvar and approx 10nm
carbon. thank you-
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From: microscopy.listserver-at-gmail.com
Date: Mon, 5 Mar 2018 19:55:35 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW:CSHL_Career_Opportunity_Research_Associate_=c2=96_?=

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Email: walbrid-at-cshl.edu Name: Samantha Walbridge

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] CSHL Career Opportunity

Message:
Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
state-of-the-art Microscopy Shared Resource.

The individual should have extensive practical expertise in biological sample preparation for
transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on
knowledge of confocal and widefield fluorescence microscopy would also be a plus.

The candidate will help users design innovative experiments and they will carry out sample
preparation and imaging as well as assist in data interpretation.

Excellent verbal and written communication skills, ability to work with multiple users in a
supporting role, and ability to work independently and proactively with limited supervision are
essential. A Bachelor’s degree in biology or related discipline is required. One to three years of
experience working in a Microscopy Shared Resource is preferred.

How to Apply

Interested individuals should apply for this position via the CSHL careers website at
http://cshl.peopleadmin.com/postings/11688

Position Number 01779-R

Applicants should include a resume along with a description of their practical expertise and the
names as well as email addresses of 3 references.

Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized
internationally for its excellence in ground-breaking research programs in cancer, neuroscience,
plant biology, genomics, and bioinformatics and broad educational mission.

For more information about CSHL, please visit us at www.cshl.edu

CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and
will not be discriminated against on the basis of race, color, religion, sex, sexual orientation,
gender identity, national origin, age, disability or protected veteran status.

VEVRAA Federal Contractor

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From: microscopy.listserver-at-gmail.com
Date: Wed, 7 Mar 2018 22:48:42 -0600
Subject: [Microscopy] viaWWW: Osmium and UA question

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute

Title-Subject: [Filtered] Osmium and UA question

Message: Dear listers.
My first question is- Is osmium better purchased made up in vials or made from crystals in the lab.
I myself prefer the vials, I feel they are safer and the solution is protected from degradation.

Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased
from Polysciences.

Thank you in advance.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 7 Mar 2018 22:49:50 -0600
Subject: [Microscopy] viaWWW: Princeton-Nature Conference: Frontiers in Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Email: nyao-at-princeton.edu Name: Nan Yao

Organization: Princeton University
Title-Subject: [Filtered] Princeton-Nature Conference: Frontiers in Electron Microscopy for the
Physical and Life Sciences

Message: I would like to ask your help for including the news of a new Nature Conference in electron
microscopy, in your future news update. Website link:
http://www.nature.com/natureconferences/fempl2018/index.html

This new conference is entitled “Princeton – Nature Conference: Frontiers in Electron Microscopy for
the Physical and Life Sciences”, to be held at Princeton, New Jersey, July 11-13, 2018. Many
internationally renowned scientists including 2017 Nobel Prize winner Dr. Joachim Frank will speak
in this conference.

Thank you for your attention. Your help in posting this in the MSA community is greatly appreciated.

Best regards,

Nan Yao


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From: peter.heimann-at-uni-bielefeld.de
Date: Thu, 8 Mar 2018 08:16:15 -0600
Subject: [Microscopy] Re: Osmium and UA question ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
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=} Osmium: I have the impression that 4% OsO4 (stored in sealed glass
ampoules under nitrogen at 4 Celsius) staines less strong after storage
for over 20 years (!); the crystalline form workswell after storage for
more than 20 years (small tip for dissolving: I snap off the tip of the
glass vial, add the water, seal tip with a little amount of
parafilm/nescofilm and sonicate in in ultrasonic waterbath for ca 5
minutes(in fumehood))


=} Uranylacetate: I use UA bought in the early nineties, works perfectly...

greetings,
Peter Heimann
( Cell Biology; Bielefeld University; Germany

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} Title-Subject: [Filtered] Osmium and UA question
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} My first question is- Is osmium better purchased made up in vials or made from crystals in the lab.
} I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Mar 2018 08:46:05 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: Osmium and UA question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Juan Luis Ribas {jlribas-at-us.es}

Dear Buchsmith,

In our hands, we always work with 1 g Osmium crystal (sealed ampoules). As we have a strong sample
preparation load in the lab,  the final solution (1-2%) is consumed in the next month aproximately,
but I suppose that it is very stable.

In Uranile case, we have been using the powder from a bottle with more than 20 years without notice
any degradation related to a new one.

In my opinion, the two substances are very stable over time.

Best regards

Juan Luis


El 08/03/2018 a las 6:08, microscopy.listserver-at-gmail.com escribió:
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}
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} I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Mar 2018 21:25:08 -0600
Subject: [Microscopy] viaWWW: Osmium and UA question

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X-from: GANG NING {gxn7-at-psu.edu}

Hi - want to share my experience with UA. About 10 yrs ago, I inherited a bottle of UA dated 1960s.
I used it to do girds staining and negative staining. It did stain without any noticeable problems
but then I got a new bottle UA of EMS from another PI, with which my staining looked fresher and
less background. The bottom line is fresh US does give better staining. Your two-year-old UA should
just be fine. Greg
----------------------------
Gang (Greg) Ning, Ph.D.
Director, Microscopy Facility
The Huck Institutes of the Life Sciences
The Pennsylvania State University N-048 MSC
University Park, PA 16802 USA
Phone: 814-863-0994
Fax: 914-867-2587
Email: gxn7-at-psu.edu
http://www.huck.psu.edu/facilities/microscopy-cytometry-up

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Sent: Thursday, March 8, 2018 12:12:12 AM

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute

Title-Subject: [Filtered] Osmium and UA question

Message: Dear listers.
My first question is- Is osmium better purchased made up in vials or made from crystals in the lab.
I myself prefer the vials, I feel they are safer and the solution is protected from degradation.

Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased
from Polysciences.

Thank you in advance.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 9 Mar 2018 11:02:10 -0600
Subject: [Microscopy] viaWWW: ORNL Seeks Technical Associate Staff Member - Scanning Probe

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Technical Associate Staff Member -
Scanning Probe Microscopy

Message: Technical Associate Staff Member - Scanning Probe Microscopy

Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff
Member to support scanning probe microscopy efforts at the Center for
Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory
(ORNL). In this role, you will be part of the Scanning Probe Microscopy
Group, which is a leader in the development and application of scanning
probe microscopies and spectroscopies to image and manipulate materials
functionality in complex materials, ionic and electronic conductors,
molecular assemblies, and nanoscale structures. A suite of 18 commercial
and internally developed atomic force microscopes in ambient and
controlled environments and scanning tunneling microscopes in ultrahigh
vacuum systems are used for internal and user science, with emerging
opportunities in enhanced data analytics and multimodal/chemical imaging.

For more details, including the qualifications for this position, visit:
http://bit.ly/TP-SPM
ORNL is an equal opportunity employer. All qualified applicants,
including individuals with disabilities and protected veterans, are
encouraged to apply.

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From: WHITTAKS-at-si.edu
Date: Fri, 9 Mar 2018 15:07:15 -0600
Subject: [Microscopy] Job Posting- micro-CT Smithsonian Institution

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Email: rvancamp-at-kettering.edu Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S

Message: We need to hasten our use of our ultramictrotome. I have
identified a few of these still in use but, need more input to find
facilities using this instrument on a daily basis that are also willing
to train me in its operation. I have made progress on my own yet, have
only been able to find one facility willing to provide training. I have
also used the manual and made progress yet, I cannot state the manual
for this instrument is well written. Finally, it is best for me to
locate facilities within the Great Lakes region. I have searched for
online training for this and have been unsuccessful.

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From purchase-at-upscmarine.com.sg Fri Mar 9 13:14:02 2018
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Once again I find myself turning to this most amazing of resources. I am casting a wide net for a micro CT operator and need your collective assistance. Many of you are closely associated with your local CT facilities or at least know the individuals providing the services. I am asking if you would be willing to pass this announcement along as I have not yet found a resource like this one in the scientific CT community.


The Smithsonian Institution National Museum of Natural History (NMNH) in Washington DC has an exciting opportunity to add a cone beam X-ray micro-computed tomography (mCT) operator in support of our new micro-CT Lab. The position is currently a 6 month contract with 2 options to extend providing for the scanning and modelling of a wide variety of natural science and museum specimens for the research staff of the NMNH.

The request for quote (RFQ) and the statement of work (SOW) are provided at the following link. Interested parties need to submit not later than COB April 13th, 2018 to be considered.

https://naturalhistory.si.edu/rc/temp/SImCToperatorRFQ-07032018.pdf

Thank you,

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
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9, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
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9, 26 -- Subject: Job Posting- micro-CT Smithsonian Institution
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From: Ying Zhang Wei :      yz32767-at-gmail.com
Date: 11 Mar 2018 10:39:16 +0000
Subject: =?UTF-8?B?5p2l6Ieq77ya5q635bCP5aeQ77yI6K+35Zue5aSN77yJ?=

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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt

Organization: Texas A&M University

Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy

Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences
at Texas A&M University seeks a highly motivated dedicated individual to work in a state-of-the-art
microscopy shared resource.

The individual must have extensive practical expertise in biological sample preparation for
transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of
widefield fluorescence microscopy and confocal and would also be a plus.
Excellent verbal and written communication skills and the ability to work with multiple users are
essential. A doctoral degree in biology or related discipline is required.
A minimum of three years of relevant experience in electron microscopy is required.

Interested individuals should apply for this position via the Texas A&M University website
https://tamus.wd1.myworkdayjobs.com/TAMU_External

Position Number: R-003044

Applicants should include a resume along with a description of their practical expertise and contact
information for 3 references.
Texas A&M University is an Equal Opportunity / Affirmative Action / Veterans / Disability Employer.



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From solizbula031-at-gmail.com Sun Mar 11 05:41:24 2018
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你好,

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英张伟。

电话:+12053514583
____________________________________________________________________
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如果您不再感兴趣,可以立即取消订阅:http://surebtcmining.com





From: microscopy.listserver-at-gmail.com
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Subject: [Microscopy] viaWWW: Associate Research Scientist - Electron Microscopy

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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt

Organization: Texas A&M University

Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy

Message: The Image Analysis Laboratory of the College of Veterinary
Medicine & Biomedical Sciences at Texas A&M University seeks a highly
motivated dedicated individual to work in a state-of-the-art microscopy
shared resource.

The individual must have extensive practical expertise in biological
sample preparation for transmission electron microscopy of animal
tissues and mammalian cell lines. Hands-on knowledge of widefield
fluorescence microscopy and confocal and would also be a plus.
Excellent verbal and written communication skills and the ability to
work with multiple users are essential. A doctoral degree in biology or
related discipline is required.
A minimum of three years of relevant experience in electron microscopy
is required.

Interested individuals should apply for this position via the Texas A&M
University website https://tamus.wd1.myworkdayjobs.com/TAMU_External

Position Number: R-003044

Applicants should include a resume along with a description of their
practical expertise and contact information for 3 references.
Texas A&M University is an Equal Opportunity / Affirmative Action /
Veterans / Disability Employer.



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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Mar 2018 14:31:56 -0500
Subject: [Microscopy] viaWWW: time on a TEM in the DFW area

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Email: snkeller-at-techie.com Name: Sandra Keller

Organization: TA-Sicco

Title-Subject: [Filtered] TEM time?

Message: Hi All:
I am looking to rent time on a TEM in good condition within a few hours
of the DFW metroplex as soon as possible. The TEM that we normally use
is down at the moment and I am looking for an instrument to use as a
backup. I have many years of TEM experience and would require minimal
training.
Thanks in advance
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Mar 2018 07:33:27 -0500
Subject: [Microscopy] viaWWW:Job at Stanford University - Transmission Electron Microscopy

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] Job Opening at George Washington University

Message: The George Washington University Nanofabrication and Imaging
Center (GWNIC) is seeking a Laboratory Associate or Technician dependent
on experience and qualifications. Duties will include preparation and
imaging of biological and materials samples for microscopy from our
research labs. Additional duties will include general laboratory
management. This is a support position in the GWNIC, for all of the
departments of the University and outside users, where we provide high
quality microscopy services. The minimum qualifications are as follows:
Laboratory Associate: A BA/BS in a related discipline plus 2 years of
relevant professional experience or, a Master’s degree or higher in a
relevant area of study. Degree must be conferred by the start date of
the position. If you are interested in this position, apply at:
http://www.gwu.jobs/postings/49493.
Laboratory Technician: A high school diploma/GED plus 1.5 years of
relevant professional experience, or, a Bachelor’s degree or higher in a
relevant area of study. Degree must be conferred by the start date of
the position. If you are interested, apply at:
http://www.gwu.jobs/postings/49611.

The George Washington University is an Equal Employment
Opportunity/Affirmative Action employer that does not unlawfully
discriminate in any of its programs or activities on the basis of race,
color, religion, sex, national origin, age, disability, veteran status,
sexual orientation, gender identity or expression, or on any other basis
prohibited by applicable law.


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From davishal182newi-at-gmail.com Tue Mar 13 06:56:38 2018
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Email: tobi-at-stanford.edu Name: Tobias Beetz

Organization: Stanford University

Title-Subject: [Filtered] Job at Stanford University - Transmission Electron Microscopy Scientist

Message: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities
The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM)
scientist to lead the operations of the facilities’ FEI Titan Environmental TEM. The TEM scientist
will provide training and support to researchers, maintain and optimize the microscope operation,
work closely with equipment vendors, maintain and develop training procedures, develop and implement
advanced techniques, assist and advice users on specimen preparation and data analysis, provide
proof-of-concept service, and engage in research activities. The SNSF Titan ETEM is equipped with a
spherical aberration corrector, EELS and EDS, a monochromator and high brightness gun, Lorentz,
holography, and tomography capabilities, as well as a suite of in situ holders. S(he) will work to
utilize this range of advanced techniques to the highest extent, making them known to the user
community, matching appropriate techniques to the individual research projects. S(he) will interact
with the broader research community and equipment vendors to be aware of advances in the field, and
make recommendations and prepare proposals for future equipment purchases. S(he) will promote and
collaborate in the publication and presentation of results and participate in conferences.
Stanford’s shared nanofacilities offer a comprehensive array of advanced nanofabrication and
nanocharacterization tools. Over 1,000 researchers make use of the shared facilities each year in
order to further their research programs. The goal of the shared facilities at Stanford University
is to provide open, cost-effective access to state-of-the-art nanofabrication and
nanocharacterization facilities for scientists and engineers from academia, small and large
companies, and government laboratories. The FEI Titan TEM is organized under SNSF in the Electron &
Ion Microscopy Suite which currently features a FEI Tecnai TEM as well as two FIB/SEMs and two SEMs.
S(he) may supervise student trainers. The TEM scientist will report to the Faculty Director of SNSF.

For more information about SNSF, visit http://snsf.stanford.edu.

Application Deadline: Applications must be submitted by April 30, 2018.

https://stanford.taleo.net/careersection/2/jobdetail.ftl?job=76088

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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Mar 2018 08:03:15 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Training Inquiry Re: Leica Reichert

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X-from: Taylor, Jordan {J.W.Taylor-at-massey.ac.nz}

Hi


I would think that you could get a lesson on any microtome and be able to use this one - especially
if it was one in the Leica family. I learnt on the Ultracut R and didn't need any training to step
up to a EM UC 7. I wouldn't limit my learning oppurtunity to just this model of microtome if I were
you - they are all essentially the same. Success will come with time and experience.


Maybe if you let the listserver know what problems you were having someone may be able to suggest a
work through for you.


Best of luck!


Jordan Taylor
Microscopy Technician
Manawatu Microscopy and Imaging Centre
Massey University
Private Bag 11-222
Palmerston North, 4442
Ph +64 6 356 9099 extn 84719


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*Sent:* Saturday, 10 March 2018 6:27:08 a.m.
*To:* Taylor, Jordan
*Subject:* [Microscopy] viaWWW:Training Inquiry Re: Leica Reichert Ultracut S



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Email: rvancamp-at-kettering.edu Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S

Message: We need to hasten our use of our ultramictrotome. I have
identified a few of these still in use but, need more input to find
facilities using this instrument on a daily basis that are also willing
to train me in its operation. I have made progress on my own yet, have
only been able to find one facility willing to provide training. I have
also used the manual and made progress yet, I cannot state the manual
for this instrument is well written. Finally, it is best for me to
locate facilities within the Great Lakes region. I have searched for
online training for this and have been unsuccessful.

Login Host: 192.138.137.220
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From: vau-at-ufl.edu
Date: Tue, 13 Mar 2018 10:20:22 -0500
Subject: [Microscopy] epoxy resin TTE and MBMCA

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Hello All,

I am planning to prepare lung tissue for TEM and Scanning Transmission X-ray Microscopy. An article by Jian Li (2009 Macromolecules 42) A new Approach to Studying Microcapsule Wall Growth Mechanisms stated they used an epoxy resin trimethylolpropane triglycidyl ether (TTE) and 4,4′-methylenebis(2-methylcyclohexylamine) (MBMCA) in a 1:1 weight ratio.

Is anyone familiar with this product? Is there a kit or would one need to purchase the reagents individually from Sigma etc?

Thank you for your suggestions
Karen Kelley, UFL



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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Mar 2018 07:37:20 -0500
Subject: [Microscopy] viaWWW:ORNL Seeks Postdoc, Scanning Probe Microscopy of Ferroic

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X-from: millerme-at-ornl.gov

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Postdoc, Scanning Probe Microscopy of Ferroic Materials in
Ultra-High Vacuum

Message: Postdoctoral Research Associate, Scanning Probe Microscopy of Ferroic Materials in
Ultra-High Vacuum

Oak Ridge National Lab’s Center for Nanophase Materials Science (CNMS) is looking for a Postdoctoral
Research Associate to support research in thin film oxide and layered materials within the Scanning
Probe Microscopy group. Our goal is to synthesize films, then measure and tune their structural,
electronic, and chemical properties with scanning probe and electron spectroscopy methods. As a
postdoc, you will contribute to research in these areas using STM imaging and spectroscopy in UHV
along with PLD growth. Research will focus on compositional and surface chemical influences on
structure and piezoelectric response in ferroic materials, particularly oxides.
For more details, including the qualifications and application for this position, visit:
http://bit.ly/ScanProbe

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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Mar 2018 07:38:00 -0500
Subject: [Microscopy] =?UTF-8?Q?viaWWW:SCSMM_symposium_=c2=96April_13=2c_2018_=c2=96Call_?=

Contents Retrieved from Microscopy Listserver Archives
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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope

Title-Subject: [Filtered] SCSMM symposium –April 13, 2018 –Call for Abstracts and Image contest

Message: Dear Fellow Microscopists:
This is to announce that Southern California Society for Microscopy and Microanalysis (SCSMM) 2018
spring symposium is to be held on Friday April 13, 2018 at the MCB/Michelson Center for Convergent
Bioscience in the main campus of University of Southern California. Speakers include Mary Scott (UC
Berkeley), Clodagh O’Shea (Salk Institute), Rebecca Voorhees (Caltech), Chongming Wang (Pacific
Northwest National Laboratory), and Cristina Zavaleta (USC). In order to register, please sign up
on-line at http://www.imri.uci.edu/content/2018-spring-meeting-registration no later than 5 p.m.
Friday, April 6.
Annual regular membership $25 (student $10). On-line registration is required.
At the symposium we will have both student platform talk and poster presentations. Please send
abstracts to Sergey Prikhodko (prikhodko.sergey-at-gmail.com) by March 18, 2018.
This year we continue to hold the SCSMM Image Contest. Please send your images to Poorna
Subramanian (poorna.physics-at-gmail.com) by April 6, 2018.
We look forward to seeing you all at the symposium.
Sincerely,
SCSMM board
www.scsmm.org
https://www.facebook.com/microscopymicroanalysis


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From: frank_karl-at-ardl.com
Date: Wed, 14 Mar 2018 10:16:25 -0500
Subject: [Microscopy] SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.

We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.

I'm open to thoughts.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: frank_karl-at-ardl.com
Date: Wed, 14 Mar 2018 11:08:10 -0500
Subject: [Microscopy] filament and bad typing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Oppsss.. Ohh damn!
I meant to say our Amray is not set up for field emission.

Sorry about the confusion........
Frank



We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is ( correction add NOT) set up for field emission.

We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.

I'm open to thoughts.




Stay safe.....

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: diller-at-stefan-diller.com
Date: Wed, 14 Mar 2018 13:34:22 -0500
Subject: [Microscopy] Re: SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

http://www.bbc.com/news/science-environment-43398187

A. John Mardinly, Ph.D., P.E.

The Sleep of Reason Produces Monsters
Francisco Goya





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From carlemau296jfsfo-at-gmail.com Wed Mar 14 11:33:57 2018
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Hi Frank,

a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called
"Spitzenkathode".

Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more
coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through
ion bombardment or burn the filament very easily.

I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a
LaB6 cathode to bring all the advantages...

Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...


Best wishes,

Stefan


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
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++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com:
} ----------------------------------------------------------------------------
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} We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
}
} We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
}
} I'm open to thoughts.
}
}
}
}
} Stay safe...........
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
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15, 31 -- From diller-at-stefan-diller.com Wed Mar 14 13:34:21 2018
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From: colijn.1-at-osu.edu
Date: Wed, 14 Mar 2018 13:36:52 -0500
Subject: [Microscopy] filament and bad typing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank et al,

It sounds like those are pointed tungsten filaments. In the days of
prehistory (aka pre-FEG) in order to get a high brightness/coherence
source people used pointed W cathodes. In order to get the tip of the
needle hot enough to have reasonable emission, the hairpin ran very hot
with the corresponding reduction in lifetime. If I remember, cathode
lifetimes of ~10 hours were common. They would have been used for
high-resolution SEM or high-coherence TEM imaging.

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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From: mike.marko.em-at-gmail.com
Date: Wed, 14 Mar 2018 14:45:09 -0500
Subject: [Microscopy] SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

I restored an Elmiskop Ia and I have a few boxes of original
Spitzenkathode. They give much more coherence -- really impressive!
But of course they don't last very long.

Does anyone know of another Elmiskop Ia still in operation?

-- Mike


On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote:
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} Hi Frank,
}
} a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called
} "Spitzenkathode".
}
} Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more
} coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through
} ion bombardment or burn the filament very easily.
}
} I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a
} LaB6 cathode to bring all the advantages...
}
} Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...
}
}
} Best wishes,
}
} Stefan
}
}
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} Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com:
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} } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
} }
} } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
} }
} } I'm open to thoughts.
} }
} }
} }
} }
} } Stay safe...........
} }
} } Frank Karl
} } Microscopist
} } Akron Rubber Development Laboratory
} } 2887 Gilchrist Road
} } Akron, Ohio 44305
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From: John.Mardinly-at-asu.edu
Date: Wed, 14 Mar 2018 16:26:30 -0500
Subject: [Microscopy] Fwd: filament and bad typing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

There is extensive discussion of pointed filaments in John Spence’s classic textbook: “Experimental High-resolution Electron Microscopy”, Oxford University Press, 1981 pp. 258-261.
}
} A. John Mardinly, Ph.D., P.E.
} The Sleep of Reason Produces Monsters
} Francisco Goya
}
}
}
} } On Mar 14, 2018, at 11:53 AM, colijn.1-at-osu.edu wrote:
} }
} }
} }
} }
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} }
} } Hi Frank et al,
} }
} } It sounds like those are pointed tungsten filaments. In the days of
} } prehistory (aka pre-FEG) in order to get a high brightness/coherence
} } source people used pointed W cathodes. In order to get the tip of the
} } needle hot enough to have reasonable emission, the hairpin ran very hot
} } with the corresponding reduction in lifetime. If I remember, cathode
} } lifetimes of ~10 hours were common. They would have been used for
} } high-resolution SEM or high-coherence TEM imaging.
} }
} } Cheers,
} } Henk
} }
} }
} } --------------------
} }
} } Hendrik O. Colijn
} } Center for Electron Microscopy and AnalysiS
} } The Ohio State University
} } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
} }
} } colijn.1-at-osu.edu 614/643-3458
} } cemas.osu.edu
} }
} } "Time is that quality of nature which keeps things from happening all at
} } once." (Ray Cummings - 1922)
} } Lately it doesn't seem to be working.
} }
} } ------ Original Message ------
} } X-from: frank_karl-at-ardl.com
} } To: colijn.1-at-osu.edu
} } Sent: 3/14/2018 12:10:25 PM
} } Subject: [Microscopy] filament and bad typing
} }
} } }
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} } }
} } } Oppsss.. Ohh damn!
} } } I meant to say our Amray is not set up for field emission.
} } }
} } } Sorry about the confusion........
} } } Frank
} } }
} } }
} } }
} } } We were recently examining a box of Amray SEM W filaments and found
} } } what we think is an odd feature on some of them. The filament is your
} } } basic loop or hairpin filament, but some have a small needle extending
} } } from one side of the wire at the apex of the point. The needed would
} } } point down the column and has the look of purposeful manufacturing.
} } } It's been suggested these are field emission filaments. Our Amray is (
} } } correction add NOT) set up for field emission.
} } }
} } } We are having trouble with filament drift in use and short lifetimes
} } } but we are reluctant to use these.
} } }
} } } I'm open to thoughts.
} } }
} } }
} } }
} } }
} } } Stay safe.....
} } }
} } } Frank Karl
} } } Microscopist
} } } Akron Rubber Development Laboratory
} } } 2887 Gilchrist Road
} } } Akron, Ohio 44305
} } }
} } }
} } }
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From: kb2612-at-columbia.edu
Date: Wed, 14 Mar 2018 17:39:43 -0500
Subject: [Microscopy] Post Doctoral Position at Columbia University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Applications are invited for Postdoctoral Research Scientist position in
the area of thin film deposition and characterization by x-ray
diffraction and electron microscopy at Columbia University. The films
will be deposited by sputtering in an ultrahigh vacuum deposition
chamber. The deposited films will be characterized by X-ray
diffraction, scanning electron microscopy conventional and
high-resolution transmission and scanning transmission electron
microscopy as well as tomographic imaging, analytical electron
microscopy and crystal orientation mapping. This is a full-time
position and the work will be carried out in the thin film deposition
lab, the shared materials characterization and electron microscopy
facilities at Columbia University and at the ASRC of the City University
of New York. The use of facilities at Brookhaven National Laboratories
is also anticipated.

For more details please see http://apam.columbia.edu/katayun-barmak

--
Katayun Barmak
Philips Electronics Professor
Director, Materials Science and Engineering Program
Department of Applied Physics and Applied Mathematics
Seeley W. Mudd Building
Columbia University
500 West 120th Street, Suite 200, MC 4701
New York, NY 10027-6623
Tel: (212) 854-8267
Fax: (212) 854-8257
Email: katayun.barmak-at-columbia.edu
URL: http://apam.columbia.edu/katayun-barmak


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From: diller-at-stefan-diller.com
Date: Thu, 15 Mar 2018 02:35:09 -0500
Subject: [Microscopy] Re: SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mike,

I did own an Elmiskop II and an Elmiskop 101 in the 80ies and 90ies.

Still I have a lot of special gaskets and o-rings, aperture holders and documents on these TEMs. Also tools etc...

If you or anybody else out there reviving an old SIEMENS machine needs parts, contact me ;-)

And: I still have some of the "Spitzenkathodes" and a lot of the normal ones.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 14.03.18 um 21:10 schrieb mike.marko.em-at-gmail.com:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Listers,
}
} I restored an Elmiskop Ia and I have a few boxes of original
} Spitzenkathode. They give much more coherence -- really impressive!
} But of course they don't last very long.
}
} Does anyone know of another Elmiskop Ia still in operation?
}
} -- Mike
}
}
} On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi Frank,
} }
} } a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called
} } "Spitzenkathode".
} }
} } Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more
} } coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through
} } ion bombardment or burn the filament very easily.
} }
} } I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a
} } LaB6 cathode to bring all the advantages...
} }
} } Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...
} }
} }
} } Best wishes,
} }
} } Stefan
} }
} }
} } -----------------------------------------------------
} } Stefan Diller - Scientific Photography
} } Arndtstrasse 22
} } D - 97072 Wuerzburg Germany
} } ++49-931-7848700 Phone
} } ++49-931-7848701 Fax
} } ++49-175-7177051 Mobile
} }
} } Websites:
} } www.nanoflight.info
} } www.stefan-diller.com
} } www.electronmicroscopy.info
} } www.elektronenmikroskopie.info
} } www.zwillingsprojekt.de
} } www.assisi.de
} } Anfahrt: http://Mail.map24.com/Stefan.Diller
} } -----------------------------------------------------
} }
} } Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com:
} } } ----------------------------------------------------------------------------
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} } } ----------------------------------------------------------------------------
} } }
} } } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
} } }
} } } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
} } }
} } } I'm open to thoughts.
} } }
} } }
} } }
} } }
} } } Stay safe...........
} } }
} } } Frank Karl
} } } Microscopist
} } } Akron Rubber Development Laboratory
} } } 2887 Gilchrist Road
} } } Akron, Ohio 44305
} } }
} } }
} } }
} } } ==============================Original Headers==============================
} } } 10, 59 -- From frank_karl-at-ardl.com Wed Mar 14 10:16:24 2018
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