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Happy New Year Colleagues;
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both jpshield-at-uga.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop
Message: This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Monday through Wednesday, March 14-16, 2018, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: March 5, 2018 ***************** Past participants include Merck & Co., Connecticut State, Breathitt Vet Center, Univ. of Chicago, Stowers Inst. for Medical Research, West Virginia State
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ben-at-protochips.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ben-at-protochips.com Name: Benjamin Jacobs
Organization: Protochips, Inc
Title-Subject: [Filtered] Applications Scientist In Situ TEM - immedate opening at Protochips
Message: Protochips has an immediate opening for a marketing applications scientist. Candidates must have a strong background in TEM, and experience with in situ TEM such as heating, gas, liquid or mechanical, is highly preferred. Travel up to 40%, mostly domestic, but some international to Europe and East Asia. Candidates must also be a US citizen or already be authorized to work in the US. Position is located in the Raleigh/Durham area in North Carolina, and candidate must be willing to relocate.
Please click the following link for more information:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both walbrid-at-cshl.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Hiring - Research Associate - Electron Microscopy Technologist Position
Message: Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual must have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
Interested individuals should apply for this position via the CSHL careers website at http://cshl.peopleadmin.com/postings/11688
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both SLC6-at-Lehigh.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: SLC6-at-Lehigh.edu Name: Sharon Coe
Organization: Lehigh University
Title-Subject: [Filtered] Lehigh University Microscopy School 2018
Message: Now accepting registrations for the 48th Lehigh Microscopy School which will be held on the campus of Lehigh University, Bethlehem, PA, June 3-8, 2018. All courses, lecturers, and instrumentation will be together for what promises to be a phenomenal week! Course offerings include: SEM and X-ray Microanalysis Introduction to SEM and EDS for the New Operator Focused Ion Beam Instrumentation and Applications Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD Data Quantitative X-ray Micoanalysis Problem Solving Using EDS and WDS Techniques Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register and pay in full by April 13 for an early bird discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu or 610-758-5133). See www.Lehigh.edu/microscopy for registration form, prices, and details about courses, lecturers, and instrument suppliers. Scholarships available for full-time graduate students.
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X-from: Guobin Hu {gb_hu-at-yahoo.com} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Hi microscopy subscribers,
We recently purchased an EMS T150 ES for carbon coating. Originally we had a standard chamber. The coating was very inconsistent. We contact EMS and EMS sent us an extended height chamber and advised us to control the coating by monitoring the FTM (Film Thickness Monitor) reading instead of by editable profiles. It does make the coating more controllable. However, the results are still not consistent. For example, I coated carbon of 9 nm by FTM and also carbon of 12.5 nm by FTM. The 9 nm thick carbon actually looked thicker than 12.5 nm. I would appreciate it if users here can share your experience with me how to coat carbon with consistent and accurate thickness. On the other hand, I would also appreciate it if you can make recommendations on carbon coaters.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both driscollg2-at-winthrop.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Title-Subject: [Filtered] Need Fiji script to subtract background noise
Message: Due to the large number of images and insufficient amount of ram, I am unable to load both image sequences (~1900 per) on Fiji in order to use the standard "image calculator" to subtract my images from the RFP (A) channel from GFP (B) channel to reduce background noise. I am looking for a script that would allow me to subtract Image 1A - Image 1B = Image 1C, Image 2A - Image 2B = 2C... etc. Ultimately, I am looking to create a Z-stack from my subtracted images to reduce background noise to further analyze data. Login Host: 206.74.212.240 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Check cooling water requirements of the SEM (flow, dissipated power) and Google for compatible chiller - there are literally hundreds if not thousands outlets selling them online, starting with amazon.com and zoro.com and ending with ebay.com and aliexpress.com; chiller for running SEM could be found with price tags below US$1,000. Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
---------------------------------------------------------------------------------------------------- *From:* "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} *To:* vray-at-partbeamsystech.com *Sent:* Thursday, January 11, 2018 9:49 AM *Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.
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-------- Forwarded Message --------
X-from: McClintock, Joel {jmcclin-at-uky.edu}
Ravi,
This only needs a flow of 1-1.5 liters/minute. Not really very much. Short term, tap water supply works fine. Risk of condensation and corrosion over time. Constantly running water, which no one likes as well.
What does flow sensor in back of misroscope indicate is the flow? YOu can change the sensor point on that flow gauge.
Pretty sure all you are cooling is the Diffusion pump. Think 700 Watt heater. I know the Neslab CFT33 is sufficient. Specs for it are "950 Watts at 20C 3240 BTU/hr at 20C 817 Kcal/hr at 20C". The CFT25 model may be not sufficient. The oldHaskris model RO33 will also work. You can find it's specs. Think mostany chiller with similar specs will work.
Joel
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Thursday, January 11, 2018 10:56:19 AM *To:* McClintock, Joel *Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.
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-------- Forwarded Message --------
X-from: Crane, Dan - OSHA {Crane.Dan-at-dol.gov}
I have a Hitachi S3500-N and have used a Haskris R-033 water cooled chiller for it. It was the recommended chiller by Hitachi. While possibly not the least expensive alternative, it has worked well for us. You can always call Hitachi High Technologies America, Inc. service department and see what they recommend.
We have used a variety of different chillers for our other microscopes and instruments, TEM, ICP, and ICP-MS. Each has to be matched to the power load of the instrument so that they are adequate and are not overworked so that they fail too soon.
The trick to know with SEM or TEM especially is that the chiller has to provide a very constant temperature with no pulsation. Temperature is important for magnification stability and absence of pulsation is essential for imaging and resolution. Not every chiller can provide either or both. You may not have seen any problems with city water for pulsation or for rapid temperature variance because of the nature of the source. However, when you go to a closed loop chiller, such problems can surface and be problematic, really degrading images.
You will need to look at the installation section of the S3500-N instrument and it will give the temperature and flow requirements for the tool. You can then go to Haskris, or any of the other suppliers and see what they can do.
Be very careful of used chillers. Pumps and motor-pump interfaces have a lot of wear, might start to pulse, and might simply give out with no warning. (Been there, done that.) Also, I just had a compressor develop what can only be called a fistula between the cooling water and the Freon lines. (We have relatively corrosive tap water.) This was an age-related failure.
I hope that this helps. Dan Crane
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, January 11, 2018 9:13 AM To: Crane, Dan - OSHA
-------- Forwarded Message --------
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Ravi,
A Haskris chiller will work well - you can get these in air-cooled versions. As for used … I don’t know. But it’s worth checking your university’s HVAC people. If they don’t have one hanging around, there might be one available surplus.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Kansas State University
Title-Subject: [Filtered] Water chiller for Hitachi SEM.
Message: We have Hitachi S3500-N SEM. A chilled water from building supply was used to run the machine, but now pressure got dropped and we are not getting sufficient flow rate to keep machine running.I don't see chances of water pressure get increased. 1. Could you guys please suggest what could be the probable suggestion ? 2. Any suggestion, which recirculating chiller will work for Hitachi S3500-N SEM? 3. If you guys can suggest any source from where we can get used chiller as our budget is limited. Note : We do not have service contract. Login Host: 129.130.145.90
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both randy-nessler-at-uiowa.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: randy-nessler-at-uiowa.edu Name: Randy Nessler
Message: I'm wondering if there is a source for the following items used in our Balzers BAF 301 freeze fracture apparatus: Bal-Tec BU 020 023-T tungsten filaments Platinum pellets BD481505 (Balzers part number) Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number) Balzers Union BD 484 058 carbon rods
Those part numbers are off some I have on hand, but I hope to add to my inventory. Searching the internet, I saw the platinum inserts listed as BU 006 103-T, carbon rod for PT/C evaporation as BU 006 101-T and the carbon rods as BU 006 115. Another listing was "carbon set" BU 020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in a publication rather than vendor inventory. Thanks for any help in advance, Randy
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both ben-at-protochips.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: ben-at-protochips.com Name: Benjamin Jacobs
Organization: Protochips, Inc
Title-Subject: [Filtered] Seeking Applications Scientists for In Situ TEM in China
Message: Protochips has immediate openings for a applications scientists located in China. Candidates must have a strong background in TEM, and experience with in situ TEM such as heating, gas, liquid or mechanical, is highly preferred. Travel up to 50%, mostly within China, but some international to Europe and the United States for training and conferences.
Please click the following link for more information:
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both unocicka-at-ornl.gov as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: unocicka-at-ornl.gov Name: Kinga Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Postdoctoral Position at ORNL on advanced and in situ STEM
Message: There is a postdoctoral position open at ORNL in the Materials Science and Technology Division on Advanced and in situ STEM (nanomechanics and catalysts)
Qualified candidates can apply through the following link: https://recruiting.ornl.gov/sap/bc/webdynpro/sap/zornl_hrrcf_c_post_doc?sap-language=EN&sap-client=010#
then search for the "Postdoctoral Research Associate in Advanced STEM Characterization / NB50650181" Posting
Purpose We are seeking a Postdoctoral Research Associate to focus on the mechanical behavior of materials at the nanoscale level, investigate the morphological changes of catalysts during their life-cycle, and work with a team to develop advanced electron microscopy analysis methods. While this position is based in our Electron Microscopy (EM) group, you'll collaborate with a diverse group of experimentalists, theorists, and data scientists across different organizations within Oak Ridge National Laboratory (ORNL). This position resides within the Materials Science and Technology Division (MSTD), Physical Sciences Directorate at ORNL.
OVERVIEW: As a postdoc, you will support the two projects indicated above while advancing data acquisition and data analysis methods for electron microscopy. The mechanical research will be directed toward the correlation of composition and crystallographic orientation with mechanical response at the nanoscale level using in situ nano-compression and scanning transmission electron microscopy (STEM). The catalytic research will focus on analyzing structural changes from the atomic to mesoscale within catalysts and correlating structure, porosity, and compositional changes with applied synthesis and other applied treatments/reactions. To do this, youll use aberration-corrected scanning transmission electron microscopy (STEM) and imaging and analytical microscopy techniques, such as electron energy loss spectroscopy (EELS) and energy X-ray dispersive spectroscopy (EDS). You'll also work with a multidisciplinary research team to develop electron microscopy characterization techniques suitable for the catalyst and quantitative data analytic methods to determine the factors that govern catalytic processes at the atomic level.
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this is what I found: ok: page document from 2009.... not knowing whether Stacey Kirsch has ready or in stock your requested consumables https://www.emsdiasum.com/microscopy/products/brochures/2009/ems2_09.pdf p.8.: Our facility is equipped to handle the following Manufacturers: } } Balzers/Baltech email: sgkcck-at-aol.com or stacie-at-ems-secure.com
or: http://www.highland-scientific.com/balzersbn.html [NB: ok, This page last updated at 11.13 on Thursday, 14 November 2013] Contact details: Highland Scientific Unit 20, Bedford Business Centre Mile Road Bedford MK42 9TW England Tel. + 44 (0)1 234 216 636 Fax. + 44 (0)1 234 271 991 Sales-at-Highland-Scientific.com
As of 25.02.2008: LEICA Microsystems buys / bought BAL-TEC in Liechtenstein (sorry only in German) http://www.chemie.de/news/78525/erneuter-firmenerwerb-durch-leica-microsyste ms-bal-tec-in-liechtenstein.html so it might be at least possible to find the rep-site of LEICA Microsystems IOWA or USA and ask them about...
Last but not least (I was able some years ago also to help another US-colleague to order parts and consumables for former BALZERS - then BAL-TEC products now BALTIC PRAEparation.e.K.: Cf: http://www.baltic-praeparation.de/ (unfortunately I found only a German webpage... Claudia Kster Baltic Prparation e.K. Koppelheck 34b D-24395 Niesgrau Telefon: 0 46 43 / 18 65 43 Telefax: 0 46 43 / 18 65 54 Mobil: 0172 / 626 44 55 Email: baltic.praeparation-at-t-online.de
I've written to them using their the php form and asked a preliminary question for you...and I am sure they will answer your request written in English...
Best wishes to you and yours, Wolfgang
======================================================= MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG sterreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA
Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Former Secretary and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} The Skin Imaging Society { www.scur.org } ====================================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 12. Januar 2018 02:03 An: wij.muss-at-aon.at Betreff: [Microscopy] Balzers BAF 301 freeze fracture consumables source --------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
please copy both randy-nessler-at-uiowa.edu as well as the Microscopy Listserver --------------------------------------------------------------------------- Email: randy-nessler-at-uiowa.edu Name: Randy Nessler Organization: University of Iowa Title-Subject: Balzers BAF 301 freeze fracture consumables source Message:
I'm wondering if there is a source for the following items used in our Balzers BAF 301 freeze fracture apparatus: Bal-Tec BU 020 023-T tungsten filaments Platinum pellets BD481505 (Balzers part number) Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number) Balzers Union BD 484 058 carbon rods
Those part numbers are off some I have on hand, but I hope to add to my inventory. Searching the internet, I saw the platinum inserts listed as BU 006 103-T, carbon rod for PT/C evaporation as BU 006 101-T and the carbon rods as BU 006 115. Another listing was "carbon set" BU 020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in a publication rather than vendor inventory. Thanks for any help in advance, Randy
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Email: jdykes-at-wvc.edu Name: Jeff Dykes
Organization: Wenatchee Valley College
Title-Subject: [Filtered] Help with ASPEX 3025 SEM
Message: Hello All,
My department just received a Scanning Electron Microscope and we are looking for some assistance. We are in need of:
-An operators manual that could be copied and sent to us. It is for a ASPEX 3025 SEM. -Guidance or protocols to prepare (fix) samples such as plants, bacteria, insects. -Any other documents, tips, etc.
Thank you,
Jeff Dykes Science Faculty Wenatchee Valley College 116 West Apple Omak, WA 98841 (USA)
jdykes-at-wvc.edu 509 422-7876
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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoc - Advanced TEM of Steel/Thin Films
Message: Postdoc position in Advanced Microscopy of Steel Microstructure and Thin Films - Position Available Now.
A postdoctoral position is available in the performance of two distinct projects. The researcher will use the state-of-the-art aberration-corrected TEM's at the Canadian Centre for Electron Microscopy (ccem.mcmaster.ca) located at McMaster University, Hamilton Canada. The CCEM is a world-class imaging facility and is equipped with 4 TEM's, including two aberration-corrected microscopes (FEI Titans). The first project is to study precipitation mechanisms of Nb-rich steels after hot rolling. This work requires knowledge of chemical imaging, diffraction methods, and an understanding of strain measurement. The second project is the study of the thin film growth process, from 2-D materials up to ALD-grown films. This requires high-resolution imaging (TEM and STEM), EELS, and EDS.
The postdoc will be part of the Bassim research group, with a strong collaboration with Prof. Zurob's research group.
Applicants should have a strong background in electron microscopy, preferably with aberration-corrected (S)TEM experience. Additional background in metallurgy or thin films is a bonus. Ability to work in a team, mentor graduate students, as well as excellent verbal and written communication skills are expected.
McMaster University is located in Hamilton, Ontario, within a 45 minute drive from downtown Toronto, which a world class city and 45 minute drive to Niagara Falls.
To apply, please send a cover letter, curriculum vitae including references, and 1-2 relevant published papers to the e-mail below.
Nabil Bassim Associate Professor Department of Materials Science and Engineering, JHE 258 McMaster University 1280 Main Street West Hamilton, ON L8S 4L8 CANADA nbassim.mcmaster.ca bassimn-at-mcmaster.ca
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Email: paul.carpenter.epma-at-gmail.com Name: Paul Carpenter
Organization: Washington University
Title-Subject: [Filtered] Solid State X-ray Spectrometry at 50 Years: Session A11 Microscopy & Microanalysis 2018
Message: Dear Microscopists and Microanalysts, It is our pleasure to inform you of: Solid State X-ray Spectrometry at 50 Years, session A11 at the 2018 Microscopy & Microanalysis Meeting, which will be held Aug. 5-9, 2018 in Baltimore, MD.
This session will concentrate on the diverse advances in hardware, software, and applications of energy-dispersive spectrometry in the last 50 years.
We are particularly interested in applications from the biological and materials communities, especially from students and young scientists.
The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018: https://www.microscopy.org/MandM/2018/
Solid State X-ray Spectrometry at 50 Years
Session chairs: Paul Carpenter, Edward Vicenzi, Kate Burgess, Nicholas Ritchie
50 years ago, Fitzgerald, Keil, and Heinrich published the first results obtained from an energy-dispersive x-ray spectrometer (EDS) in Science. From identification of unknown materials, to compositional mapping and quantitative microanalysis, EDS has advanced our understanding of an enormous range of materials and is used worldwide in microanalysis and microscopy laboratories. The symposium will link historical and technical developments of solid state x-ray instrumentation, data processing, applications, and emerging detection systems. Perspectives on the developments in EDS from a technological and educational perspective will be featured, including invited and contributed presentations from the inventor, vendor, and scientific communities.
o State-of-the-art solid state x-ray detector instrumentation o Soft x-ray and light element analysis in the field emission SEM o Nano to atomic resolution microanalysis by STEM o Quantitative analysis methods - limits and limitations o Spectrum imaging and data processing o Complementary methodologies including micro-XRF, combined WDS-EDS, XRD, and EBSD
Paul Carpenter, Washington University
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Email: klemari-at-fzu.cz Name: Mariana Klementova
Organization: Institute of Physics of the Czech Academy of Sciences
Title-Subject: [Filtered] Lorentz microscopy - position
Message: Dear colleagues,
we are currently looking for a microscopist who would be able to introduce Lorentz microscopy to our group at the Institute of Physics. We have a Tecnai F20 X-twin with a Lorentz lens. We have got funding for the position for 6 months. The position will be available from June 1, 2018.
If you are interested please contact me by January 31, 2018.
With best regards,
Mariana Klementova
************************************************ Mariana Klementova ************************************************ Institute of Physics of the CAS, v.v.i. Na Slovance 1999/2 CZ-182 21 Praha 8 Czech Republic ************************************************ tel. : +420 - 266 05 2612 email: klemari-at-fzu.cz ************************************************
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Email: kandi-at-isearchbio.com Name: Kandi Williams
Organization: K.L. Williams & Associates
Title-Subject: [Filtered] Entry level microscope sales - San Francisco and Seattle
Message: We are currently working with a major manufacturer of laboratory microscopes and related optical products to assist them in filling a San Francisco and a Seattle based entry level microscope sales positions. We are looking for a young, energetic scientist with a year or two of post doc experience, or several years post graduate lab management experience, with heavy confocal experience using, teaching, troubleshooting. This would be an excellent first step off the bench into industry. The company will provide excellent training and mentorship and a solid career path. First year salary package is anticipated to be around $100k plus the company provides a monthly car allowance, covers all business and travel expenses and has an excellent benefits package. Overnight business travel for both territories is low, maybe 2 to 3 nights/month, primarily for meetings and training. Please note that the company is unable to provide any sort of visa sponsorship at this time. Take care, Kandi Williams
K.L. Williams & Associates P.O. Box 5421 Auburn, CA 95604-5421 (530) 885-3693 kandi at isearchbio.com
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Hello, I recently purchased a Wig-L-Bug grinder/mixer, an agate vial and an adapter to hold the vial. I am at a loss as to how the vial is held by the adapter and the adapter certainly does not fit on the arms of the grinder/mixer. I made three attempts at contacting the manufacturer's technical support but to no avail. Hence this plea for help. If any of you have this setup, I appreciate your input. I can share photos of the parts if that would help.
BTW, the purpose for the agate vial is for grinding ashed materials that often contain silicates.
Tom Kremer SGS-IPS Testing Appleton, WI Information in this email and any attachments is confidential and intended solely for the use of the individual(s) to whom it is addressed or otherwise directed. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of the Company. Finally, the recipient should check this email and any attachments for the presence of viruses. The Company accepts no liability for any damage caused by any virus transmitted by this email. All SGS services are rendered in accordance with the applicable SGS conditions of service available on request and accessible at http://www.sgs.com/en/Terms-and-Conditions.aspx
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Email: mplattsc-at-gmail.com Name: Michael Platt
Organization: University of South Carolina
Title-Subject: [Filtered] Vac check connection failure, Zeiss Leo SEM
Message: I have been working on this issue, along with an L-REM failure off and on for a while and have gotten nowhere. I have been concentrating on the L-REM failure but now I want to look harder into trying to solve the Vac Check Connection fail. Those failures show up during the power up initialization. First, what does that really mean? Not that it has anything to do with the failure but the backing pump and turbo pump both come on and I believe the turbo comes up to speed, solid green light on the controller. The vacuum gauge's green lamp is also on. Regarding previous suggestions I have check every fuse I could find, all good. All fiber optic cables appear to be OK on the L-REM and associated units. Tests indicate the power supply failures on the EO and PU boards but where exactly are they as I could find only numeric id's for all the PC boards.
Suggestions?
Michael Platt
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Title-Subject: [Filtered] The Rockefeller University seeks Electron Microscopy Specialist
Message: Message: Electron Microscopist, The Rockefeller University, New York, NY
The Rockefeller University, a premier biomedical research institution, seeks a Research Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).
The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is equipped with three transmission electron microscopes, a conventional and a serial block-face imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit. (http://www.rockefeller.edu/emrc/)
The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations, including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering supplies, interacting with vendors, and administrative support for office duties, including center billing. The position also entails specimen preparation, including negative staining, ultrathin sectioning, and immunolabeling, operation of the microscopes and associated equipment, training users, as well as consulting scientists on the design of experiments, data processing/analysis, interpretation of results, and informing users on the latest methodology through familiarity with relevant literature.
The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology, bioengineering or a related field and must have a minimum of 5 years of hands-on experience in electron microscopy. A strong background in computation would be a plus. Must have strong communication skills, and the ability to work collaboratively in a team as well as independently on a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.
We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to this job, click the following URL, click on 'staff opportunitiesf and enter keywordeIRC20449f or eElectron Microscopistf: http://www.rockefeller.edu/hr/career.php
The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.
Regards, Hiro ------ Kunihiro Uryu, Ph.D., Research Assistant Professor Director of Electron Microscopy Resource Center (EMRC) RRB Rm120 The Rockefeller University 1230 York Ave., Box 230 New York, NY 10065
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Email: lamt-at-si.edu Name: Thomas Lam
Organization: Smithsonian Museum Conservation Institute
Title-Subject: [Filtered] P08 Spectroscopic and Imaging Studies in Heritage Science
Message: It is our pleasure to inform you there will be a Spectroscopic and Imaging Studies in Heritage Science symposium, session P08 at the 2018 Microscopy & Microanalysis Meeting, which will be held Aug. 5-9, 2018 in Baltimore, MD.
The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018: https://www.microscopy.org/MandM/2018/
P08 Spectroscopic and Imaging Studies in Heritage Science
The application of microscale and nanoscale characterization techniques to the examination of cultural heritage materials has greatly enhanced our understanding of the processes that formed, and subsequently transformed those materials to their present state. Understanding the chemistry and morphology of heritage materials from the macro/mesoscopic scale to the microscale is of critical importance for our increasingly deeper levels of knowledge of the interaction between objects and their environment. This symposium will include invited and contributed presentations from students, conservators, conservation scientists, researchers, and those from other disciplines who have an interest in the preservation of cultural heritage.
o Emerging methods for microscale and nanoscale examination in culture heritage o Case studies on historic and prehistoric materials o Non-invasive and minimally-invasive imaging and analysis methods o Kinetics of light fastness using microfadeometry o Synchrotron- and neutron-based studies o New approaches to protective coatings for heritage objects ________________________________________
Students, postdocs, and technical staff All full-time students enrolled at accredited academic institutions and all full-time postdoctoral fellows are eligible for meeting award support. High school, undergraduate, and graduate students are encouraged to apply. Applicants are not required to be members of the sponsoring societies. Additionally, full-time technologists are eligible for support as well if the applicant is a member of the sponsoring society, current in his or her dues for the year of the meeting.
We look forward to seeing you at the Baltimore inner harbor next year! On behalf of the symposium organizers,
Edward P. Vicenzi (Smithsonian Institution) John Mansfield (University of Michigan) Thomas Lam (Smithsonian Institution)
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Organization: Northern California Society for Microscopy
Title-Subject: [Filtered] NCSM Spring Meeting in Pleasanton, CA
Message: Northern California Society for Microscopy announces its spring meeting on March 27 at the Zeiss Customer Center in Pleasanton, CA. MSA guest tour speaker is: Sara Miller, PhD. of Duke Medical Center. Presenting: Emerging Diseases and Microscopy
More info at: ncsmicroscopy.org
RSVP: info-at-ncsmicroscopy.org
We hope you will attend.
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Name: Janine Hernandez School: San Joaquin Delta College Grade/Education Level: Undergraduate Location: Stockton, CA Email: janine.hernandez182-at-gmail.com
(Listserve - I apologize - I think I did the reply incorrectly at first)
Hi Janine,
Powders and pigments likely do not require encapsulation. It's usually much easier to place them on a supporting grid directly for imaging. This of course assumes that the size of the powder or pigment is small enough for electron transmission. Often it's worth it just to check incase it is possible; which makes sample prep fairly easy.
There are a few methods that could be used to prepare these- one that I've had some success with fine powders in the past is as follows: place an amount of the powder into isopropanol, ultrasonicate to break up agglomerations, dip a holy-carbon grid into the liquid, pull it out, and allowing the grid to air dry. If there was a small enough amount, but enough to be finely dispersed in the alcohol, you will pick up some on the grid. This grid, when dried, can then be imaged with the powders suspended on the holy-carbon. It may take a few trials to get enough from the alcohol suspension. Another method that has worked for me in the past (assuming the powder is well separated) is a very light dusting of the powder mid-air above the grid (allowing some of the powder to fall down onto the grid on the table). This one is less likely to obtain separated powders that are small enough to image, but I have seen some success with it as well in the past. (Use of a clean fine artist paint brush to flick the powder into the air above the grid may work here.) Please be careful not to allow the alcohol with the powder or the powder in air to get on your skin or to be breathed in. Some solvents (acetone notably) allows chemicals to enter through the skin, and we are learning that nanopowders are not healthy for you to breathe in as well.
I hope this is helpful. You'll likely get a number of great answers from the list-serve and I'm looking forward to learning from their answers also!
Wishing you a grand time exploring small worlds! -Allen J. Hall Prairie Nanotechnology www.prairienanotech.com
Senior Postdoctoral Fellow in the Canadian Centre for Electron Microscopy
The Canadian Centre for Electron Microscopy (CCEM) is a national facility located at McMaster University, in Hamilton, ON, Canada. The CCEM operates four TEMs, including two FEI Titan TEMs, equipped with image and probe correctors and monochromators, as well as a Quantum GIF equipped with K2 direct electron detector for spectroscopy.
We are seeking a skilled and motivated researcher to perform a variety of imaging and analysis experiments supporting the needs of academic and industrial users, including consulting, planning of experiments, data processing and interpretation. An important role of this position is the training of users on the instruments.
Candidates should have a PhD in Physics, Material Science/Engineering, Chemistry or a related field with demonstrated hands-on experience using transmission electron microscopes and EDS/EELS, preferrably Titan TEMs. Strong communication skills and the ability to develop successful data acquisition and processing strategies will be essential in this role. Experience in data processing and scripting in Python or Matlab, as well as a demonstrated track record of research and interactions with users would be an asset.
To apply, please send your cover letter and resume to korinek-at-mcmaster. ca
-- Dr. Andreas Korinek
Manager
Canadian Centre for Electron Microscopy Brockhouse Institute for Materials Research McMaster University 1280 Main Street West, Hamilton ON Canada, L8S 4M1 phone: +1 905-525-9140 ext 20400
==============================Original Headers============================== 10, 38 -- From korinek-at-mcmaster.ca Mon Jan 29 15:19:13 2018 10, 38 -- Received: from FHSHC4H16-2.csu.mcmaster.ca (fhshc4h16-2.CSU.McMaster.CA [130.113.22.4]) 10, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w0TLJD6e030414 10, 38 -- for {microscopy-at-microscopy.com} ; Mon, 29 Jan 2018 15:19:13 -0600 10, 38 -- Received: from FHSDB2D11-2.csu.mcmaster.ca ([fe80::2924:2ca:3cbc:4521]) by 10, 38 -- FHSHC4H16-2.csu.mcmaster.ca ([2002:8271:1604::8271:1604]) with mapi id 10, 38 -- 14.03.0319.002; Mon, 29 Jan 2018 15:14:11 -0500 10, 38 -- From: "Korinek, Andreas" {korinek-at-mcmaster.ca} 10, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 38 -- Subject: Job Opening: Senior Postdoctoral Fellow in the Canadian Centre for 10, 38 -- Electron Microscopy 10, 38 -- Thread-Topic: Job Opening: Senior Postdoctoral Fellow in the Canadian Centre 10, 38 -- for Electron Microscopy 10, 38 -- Thread-Index: AQHTmT25Tf/tzryPHUm6S/U4i2qljQ== 10, 38 -- Date: Mon, 29 Jan 2018 20:14:10 +0000 10, 38 -- Message-ID: {1517256849.24146.8.camel-at-mcmaster.ca} 10, 38 -- Accept-Language: en-CA, en-US 10, 38 -- Content-Language: en-US 10, 38 -- X-MS-Has-Attach: 10, 38 -- X-MS-TNEF-Correlator: 10, 38 -- x-originating-ip: [130.113.22.232] 10, 38 -- x-tm-as-product-ver: SMEX-12.5.0.1300-8.2.1013-23628.002 10, 38 -- x-tm-as-result: No-5.608800-5.000000-10 10, 38 -- x-tmase-matchedrid: SbnkZF0UrmA/mJEvNFL+dY+emiGnyeDR4Pjj54kIW/GVvB3MWg1bhKjP 10, 38 -- dtRFsI/Ttpq8RF54CmTUqlNduJHbX6ZJF6TEqRucQjDDVgDayL60xIzVr7Ulb0eD57cU53Gilpf 10, 38 -- gNtKoBw6qYaQyxONnx5Zd8LXqzTgbPHPcxToV+PdCZSbGz8XXg1z85UQdvE4EFNu748/lj/LCBr 10, 38 -- VgCBNlqSVyXfqCUtLRSy+kn1dQUY2yl+gKyy/g7xyFKiDPvSEg/kqO2xYqX5Y7g4/7QP0AlqPFj 10, 38 -- JEFr+olA6QGdvwfwZbkwjHXXC/4I8prJP8FBOIavByPQY4m0tDBOwgZ87U4NKl3cNQsLUXRoR/L 10, 38 -- CWpMc6W2H0DwvnL5XsC+ksT6a9fy 10, 38 -- x-tm-as-user-approved-sender: No 10, 38 -- x-tm-as-user-blocked-sender: No 10, 38 -- x-tmase-result: 10--5.608800-5.000000 10, 38 -- x-tmase-version: SMEX-12.5.0.1300-8.2.1013-23628.002 10, 38 -- Content-Type: text/plain; charset="utf-8" 10, 38 -- Content-ID: {8FEBFC9E8893F64886477620D22C54C5-at-fhsadmin.csu.mcmaster.ca} 10, 38 -- MIME-Version: 1.0 10, 38 -- Content-Transfer-Encoding: 8bit 10, 38 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w0TLJD6e030414 ==============================End of - Headers==============================
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Email: psicurello-at-ucsd.edu Name: Paula Sicurello
Organization: UCSD
Title-Subject: [Filtered] Zeiss 10C available
Message: Please post for me this Zeiss 10C that we are giving away.
Hello Everybody,
Free to a good home.
Is anyone interested in a vintage Zeiss 10C? This scope is in working condition and has been lovingly maintained. It comes with a side mounted Gatan ES1000W camera. The purchaser will be responsible for the costs associated with the disassembly and removal.
Please contact me if you are interested.
Sincerely,
Paula Sicurello, HTL (ASCP)
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
psicurello-at-ucsd.edu P: 619-583-2872
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health 200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
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Email: cjbray-at-es.utoronto.ca Name: Colin Bray
Organization: Earth Science, Univ. of Toronto
Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed
Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the vacuum system driver board. he has circuit diagrams for most of the other units but naturally, not the one he needs. Can anyone help?
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Dear colleagues - to commemorate the decade of PFM conferences, I have made the integrated set of slides (~350) on the principles and applications of piezoresponse force microscopy and spectroscopy based on tutorials on ~20 PFM workshops and conferences over last decade.
There are 7 parts, including: 1. Introduction to PFM and Nanoscale electromechanical phenomena 2. Contact mechanics and image formation in PFM 3. Cantilever dynamics in PFM 4. PFM of ferroelectric materials and local polarization switching 5. Switching spectroscopy piezoresponse force microscopy 6. Advanced time- and voltage spectroscopies in PFM 7. PFM in polar and nonpolar liquids
If interested, please contact me directly at sergei2-at-ornl.gov {mailto:sergei2-at-ornl.gov} . Also, please forward this information to your colleagues interested in PFM.
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 9, 37 -- From sergei2-at-ornl.gov Wed Jan 31 10:54:42 2018 9, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 9, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w0VGsfQH003732 9, 37 -- for {microscopy-at-microscopy.com} ; Wed, 31 Jan 2018 10:54:42 -0600 9, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 9, 37 -- t=1517413785; x=1548949785; 9, 37 -- h=to:from:subject:message-id:date:mime-version: 9, 37 -- content-transfer-encoding; 9, 37 -- bh=WK+xz8dtT86FfuyGPtcrR3ftshOsu+zFz8iEyFxfATc=; 9, 37 -- b=OCYQOz85CMSU7QUTt9NfeT+/Nv/sqdS4jpaL/U0hrOxdMoCzX42sMvtA 9, 37 -- vvZEYX1p+KdQfY/tgJMqN71r5xI1FDs0UVsy7XwmTFmIBx6ekNEHOBcrZ 9, 37 -- p0GfJ4axuwlnglWq1ZLTJasA/Ac47IL4RYoUa6c3FkMteCM5GVUejmWmz 9, 37 -- +DvMlf9gZIWqCphesnE3Y7M8rn0tzhW/jHKP658Eopz3mtttuLcW6lS4B 9, 37 -- W8uX1WVK6kluGpbwJ3rVVAimy3Kumio0dJZ02XF2oUdH3Y39pa6o9Wfai 9, 37 -- quwezOH12djQDjmyVc334jGkZCYMHQXb6TqQ8CG6qOuHDwQmZQdBIBuP2 9, 37 -- w==; 9, 37 -- X-SG: RELAYLIST 9, 37 -- X-IronPort-AV: E=Sophos;i="5.46,440,1511845200"; 9, 37 -- d="scan'208";a="31016179" 9, 37 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 9, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 31 Jan 2018 10:49:45 -0500 9, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 9, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 9, 37 -- (No client certificate requested) 9, 37 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 3zWnkK28c7z2TF1s 9, 37 -- for {microscopy-at-microscopy.com} ; Wed, 31 Jan 2018 10:49:45 -0500 (EST) 9, 37 -- To: microscopy-at-microscopy.com 9, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 9, 37 -- Subject: Piezoresponse Force Microscopy tutorial - integrated slides 9, 37 -- Message-ID: {5A71E598.8000501-at-ornl.gov} 9, 37 -- Date: Wed, 31 Jan 2018 10:49:44 -0500 9, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 9, 37 -- Thunderbird/38.0.1 9, 37 -- MIME-Version: 1.0 9, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Ken Converse used to support ETECs, the bits of contact info I have are:
QUALITY IMAGES Servicing Scanning Electron Microscopes Since 1981 474 So. Bridgton Rd. Bridgton, ME 04009 207-647-4348 Fax 207-647-2688 kenconverse-at-qualityimages.biz {mailto:kenconverse-at-qualityimages.biz} qualityimages.biz
Quality Images, /Ken Converse/, Maine, from NC to ME to OH, SEM, Amray//Etec//Any, (717) 456-5491
Good luck! Valery Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
---------------------------------------------------------------------------------------------------- *From:* "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} *To:* vray-at-partbeamsystech.com *Sent:* Wednesday, January 31, 2018 9:08 AM *Subject:* [Microscopy] viaWWW: ETEC Autoscan SEM circuit diagrams needed
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Email: cjbray-at-es.utoronto.ca {mailto:cjbray-at-es.utoronto.ca} Name: Colin Bray
Organization: Earth Science, Univ. of Toronto
Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed
Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the vacuum system driver board. he has circuit diagrams for most of the other units but naturally, not the one he needs. Can anyone help?
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Email: tstern-at-princeton.edu Name: Tomer Stern
Organization: Princeton University
Title-Subject: [Filtered] Beads size and intensity
Message: Hi all,
I'm using a MuVi-SPIM, similar to the Luxendo model.
To register the different views and also to infer the PSF prior to deconvolution I'm using beads, which are scattered around the sample - inside the agarose - and are being scanned at the same time as the sample.
I have two questions:
1. Is the intensity of the beads relative to the intensity of the marker inside the tissue important for the inference of the PSF? Meaning, is it better to use beads with intensities that are similar to the sample, or should their intensity be higher / lower / it doesn't really matter?
2. As far as I know the preferred diameter of the bead is one that is a bit smaller than the size of the voxel in the XY plane, right? if so, then if I know that I'm going to use a 2x2 binning simply by averaging every 2x2 block in each slide, should I still use the same size of beads, or should it match the new size of the 'binned' - enlarged - pixels?
Thanks in advance! Tomer
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Berkeley Lab’s Molecular Foundry Division has an opening for a Scientific Engineering Associate or Sr. Scientific Engineering Associate. The Molecular Foundry is a Department of Energy-funded nanoscience research facility that provides users from around the world with access to cutting-edge expertise and instrumentation in a collaborative, multidisciplinary environment. As one of the seven facilities in the Molecular Foundry, The National Center for Electron Microscopy (NCEM) serves a broad user community in materials science by supplying unique instrumentation, expert advice, assistance, and training in advanced electron microscopy techniques.
Diversity, equity, and inclusion are core values at the Molecular Foundry. Therefore, we seek candidates whose research, past employment, or service has prepared them to contribute to our commitment to diversity and inclusion. Our excellence can only be fully realized by staff who share our commitment to these values. Successful candidates for our staff position will demonstrate either potential to contribute or evidence of a commitment to equity and inclusion.
This position provides highly specialized expertise, consultation, and contributions to project process and knowledge in support of in situ transmission electron microscopy (TEM) experimentation and TEM sample preparation. This includes the development and optimization of techniques for preparing high resolution TEM samples from a wide variety of materials and for in situ TEM investigations, user assistance and training, technical proposal evaluation, and collaborations on scientific projects. The position is responsible for scheduling, maintenance, operation and technical development of the sample preparation laboratories and in situ TEM experimental equipment. Will provide resolution to a diverse range of moderately complex technical problems and participate in, and in some cases lead, experimental methodology development and project execution. Classification will depend upon the applicant's level of skills, knowledge, and abilities.
For further details and to apply please go to the following link:
http://m.rfer.us/LBLYNuJJ
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Title-Subject: [Filtered] Postdoctoral Fellow In situ cryo-EM
Message: Dear list members,
In my lab, a position of in situ cryo-EM in the area of structural cell biology is available. I am still looking for a candidate with experience in cryo-ET/EM and/or FIB-SEM for the following project: http://s.embl.org/HD01243
In case you have further questions, please do not hesitate to contact me. Closing date for applications is Feb 16.
Best wishes,
Carsten ____________________________________________________________________________________ Dr. Carsten Sachse Group Leader European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany email: carsten.sachse-at-embl.de http://www.embl.de/research/units/scb/sachse/ http://www.sachse.embl.de
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Hello Everyone, I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.
Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!
Thanks!!!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
I've had a similar issue with our FEI Morgagni 267D (for most purposes similar to the CM12). I'd bet the wehnalt assembly is almost identical. For us it was almost certainly a filament break in such a way that it was shorting across the wehnalt. I say "almost certainly" because in our case this happened at almost the same time that a bunch of electronics issues occurred and so we had mixed symptoms. But replacing the filament (which we had only just replaced as part of our diagnosis of elimination), did the trick for us.
At least it is a simple check.
Good luck, Duane
Duane Harland Senior Scientist Food & Bio-based Products T +64 3 321 8710 Based at Lincoln Research Centre Campus agresearch.co.nz New Zealand
-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: Thursday, 8 February 2018 9:10 AM To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}
Hello Everyone, I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.
Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!
Thanks!!!
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Frank, This is called Dark Current. It comes from the evaporation of filament material onto the insulator base creating a pathway for electrons to leak to ground, and therefore, creating a current in the filament that causes it to glow with only the kV on. It’s not a problem, but indicates that your filament is old. Ken
Kenneth JT Livi, PhD Director, Materials Characterization and Processing Center Materials Science and Engineering 3400 N Charles Street Johns Hopkins University Baltimore, Maryland 21218 USA
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A collegue in the lab has strong health difficulties with her shoulders and wrists. She works on a Zeiss Axio Imager D2m with a special stage and external accessories which leaves only a close access to the focusing knobs. So we are looking for a companies which could fit a motorization on the focus knobs, to have a remote control of them. Does someone know a (preferably french or europeen) company which could do this job ? We'll ask Zeiss too, but we have some fear about the price...
Thanks in advance Jacques
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
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Email: gary-at-cermetmaterials.com Name: Gary Castelow
Organization: CERMET MATERIALS INC.
Title-Subject: [Filtered] JEOL 840A SEM
Message: Hello all! We randomly lost video signal with our SEM the other day. Acted as if the filament had blown, but the check gauge is still reading current when the AC is turned on. Im am by no means an "electrician" but all connections still look ok going to the viewing monitor. Is there something fairly easy that goes bad I could check?? Or any other help is greatly appreciated!
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Email: wschneider-at-wisc.edu Name: Bil Schneider Organization: UW Madison Title-Subject: [Filtered] Tungsten filaments
Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does anyone have any suggestions for extending filament life? We try and operate slightly under saturated except for EBSD or EDS mapping. Ive heard of seasoning the filament but have never seen an actual protocol or talked with anybody whos actually done it. Any suggestions would be appreciated!
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did you check your complete video-processing path, like:
- setting the gain of the SE-detector so high to finally see the signal noise (this would state at least a working photomultiplier / detector system)
- did you check for the cage voltage switch to be on "positive" ?
- check on HV voltage and cathode heating. Do you see any change by going up in heating?
- centering and e-beam shift potis aligned?
- cathode OK? Current might be a dark currant or mis-functioning high voltage unit
- take out the final aperture and try to find beam...
These are only a few possibilities...
Best wishes,
Stefan
-
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Bill, with " } seasoning { a tungsten filament" you perhaps (might) think of slow and careful -controlled 'heating' of a new filament (as I have done that with every new filament after mounting in a TEM, a ZEISS109 with differential pumping system=rotary pump -for RV as well as an Ion Getter Pump for HV).
IMHO 'slow heating' of the cathode filament before subjecting it to the "hard" working conditions might be (at least in my experience really 'is' ) of benefit regarding beam stability as well as maintaining your filament's lifetime.
"Slow heating" means [after change of filament] at least running the new filament 1-2 hrs 'under-under'-saturated after achievement of an evacuation optimum of the chamber (not knowing whether you could see anyhow the filament's image in such a condition like in a TEM on the screen). You easily would / could see (if you e.g. have permanent vacuum-measurement monitoring ready) that on starting to "heat"(increase) the filament (current), the vacuum decreases significantly (at least slightly) because of "evaporation" of metal & impurities / contaminants.
Then increase filament current a bit more.... and let "equilibrate" vacuum conditions. You might even find such decreasing vacuum after a third increasing of the filament heating near the 'saturation' point.
Another helpful requisite also would be to use - without using or even heating the filament first an ACD (anti-contamination device - if available) with at least one if not two cycles of cooling first and pumping the chamber afterwards to get rid of most organic molecules you brought into the filament chamber by your cleaning and mounting procedures - let warm up the ACD and pump (RV and HV if possible) again. According to the specifications of the Hitachi S3400N VPSEM (I found on: https://www.ntnu.edu/documents/140082/1269041159/S-3400NSpecifications.pdf/6 b94c26f-a9d4-4f36-82b3-7e9eb3603922 ) the possibility of doing so might be impossible due to the automatic settings of diverse functions but you should know about those possibilities from practical experience.
Unfortunately a modern VPSEM is not comparable to such an old piece of equipment like the TEM ZEISS 109 (vintage 1976) I worked with where maintaining and handling vacuum matters relatively easy... And, BTW, life time of a tungsten filament always depends also on the "technical quality" and the handling - when mounting into the chamber - too....
Best wishes and good luck, Wolfgang
MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired] A-5020 SALZBURG sterreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
============================================================================ ================================= Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 9. Februar 2018 10:29 An: wij.muss-at-aon.at Betreff: [Microscopy] Tungsten filaments [Hitachi S3400N VPSEM, how extending filament life?] ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
please copy both wschneider-at-wisc.edu as well as the Microscopy Listserver --------------------------------------------------------------------------- Email: wschneider-at-wisc.edu Name: Bil Schneider Organization: UW Madison Title-Subject: Tungsten filaments Message:
We use tungsten filaments in our Hitachi S3400N VPSEM. Does anyone have any suggestions for extending filament life? We try and operate slightly under saturated except for EBSD or EDS mapping. Ive heard of seasoning the filament but have never seen an actual protocol or talked with anybody whos actually done it. Any suggestions would be appreciated!
First, what kind of filament life are you currently getting? If you are close to 100 hours, I would be satisfied. If you are only getting 30, then I would look for a problem.
We got better life when we ran at a consistent voltage and didn't have users doing the saturating. We went from average lifetimes of 25 hours to 80 hours. We also operated just under saturation conditions. We simply turned off the high voltage at the end of the session.
We needed to check saturation and reduce current over time. As the filament thinned, it needed less current to reach the same temperature.
Finally, we ran the filament a little further back from the front of the wehnelt. That lessened brightness but increased life.
Warren ________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Friday, February 9, 2018 3:09:18 AM To: Straszheim, Warren E [BIOTC]
X-from: wschneider-at-wisc.edu
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Email: wschneider-at-wisc.edu Name: Bil Schneider Organization: UW Madison Title-Subject: [Filtered] Tungsten filaments
Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does anyone have any suggestions for extending filament life? We try and operate slightly under saturated except for EBSD or EDS mapping. I've heard of "seasoning" the filament but have never seen an actual protocol or talked with anybody who's actually done it. Any suggestions would be appreciated!
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Name:karl Hagglund School:Northwestern University Grade/Education Level:Graduate Location:Evanston, IL US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject} Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board Your Question: Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts. Thank you, Karl Hagglund
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Hi Karl, If you're in a rush, I would start by contacting these after-market providers: http://www.jcnabity.com/links.htm#Digital Imaging
If you aren't in a rush, I would find an Electrical Engineering student who wants a long-term project (lots of EE programs have this as a graduation requirement) and get them to build one for you (and ideally require they publish their methods openly). They can start with this for inspiration and basics:
Electron microscope image capture with an oscilloscope https://www.youtube.com/watch?v=SWVu-qPR-Ws
Electron microscope image capture via microcontroller (with drill bit animation) https://www.youtube.com/watch?v=ruuxn2u3yao http://benkrasnow.blogspot.com/2015/08/drill-bit-in-electron-microscope.html
DIY Scanning Electron Microscope - Sources, Costs and References http://benkrasnow.blogspot.com/2011/03/diy-scanning-electron-microscope_26.html
SEM data files and image generation script http://benkrasnow.blogspot.com/2014/11/sem-data-files-and-image-generation.html
On Fri, Feb 9, 2018 at 3:18 PM, {oshel1pe-at-cmich.edu} wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } } } } } } Name:karl Hagglund } School:Northwestern University } Grade/Education Level:Graduate } Location:Evanston, IL } US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject} } Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board } Your Question: } Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts. } Thank you, } Karl Hagglund } } } } } } ==============================Original Headers============================== } 10, 59 -- From oshel1pe-at-cmich.edu Fri Feb 9 16:58:07 2018 } 10, 59 -- Received: from NAM01-BN3-obe.outbound.protection.outlook.com (mail-bn3nam01on0113.outbound.protection.outlook.com [104.47.33.113]) } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19Mw62x009150 } 10, 59 -- for {microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 16:58:07 -0600 } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 59 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 10, 59 -- bh=UKlhs70T8N2RchOoD5XEkqH/C+5CkuB8zI2H8YME/7A=; } 10, 59 -- b=OgcR5ZQlZB2cg+Lc6nMDHbX6DkhW7M4aONwtOxdVLewFCgsiaHth+fj0YCWJArD0hez8aScoYPyEYAu/KQoV5ghtez33mhoajIdyA2HS8ebvmJ8L6qqXhifJsk3JV6qes9Q6xcUuKxbS84wscL0olahXsoPYxBx/pYrk9x57rW0= } 10, 59 -- Received: from CY1PR0501MB1676.namprd05.prod.outlook.com (10.163.140.15) by } 10, 59 -- CY1PR0501MB1386.namprd05.prod.outlook.com (10.160.148.140) with Microsoft } 10, 59 -- SMTP Server (version=TLS1_2, } 10, 59 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.20.485.3; 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-- -Nathan
==============================Original Headers============================== 13, 47 -- From nmz787-at-gmail.com Fri Feb 9 17:55:34 2018 13, 47 -- Received: from mail-pg0-f65.google.com (mail-pg0-f65.google.com [74.125.83.65]) 13, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19NtYjt004471 13, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 17:55:34 -0600 13, 47 -- Received: by mail-pg0-f65.google.com with SMTP id t4so3515637pgp.8 13, 47 -- for {Microscopy-at-microscopy.com} ; Fri, 09 Feb 2018 14:51:08 -0800 (PST) 13, 47 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 47 -- d=gmail.com; s=20161025; 13, 47 -- h=mime-version:in-reply-to:references:from:date:message-id:subject:to 13, 47 -- :content-transfer-encoding; 13, 47 -- bh=1guh5Wa2GBJEuLGDKB/Nj/MQijUEXpp4rJ0ZVHmFo8g=; 13, 47 -- b=P/ZQlkbBEju7uM6Vu4HyR5fV51Iy/GTNIB4VyhFGIfdYBkZ9qy38oQcfrfM4BO4g5F 13, 47 -- kCpq+uFTZnuB0okV/iDVfu4sTkibLlmF7JpwZPZmkPsr6lIr++ntPXLlDG5N0WIJotwT 13, 47 -- FX0vLmalzvSiBHe7ClA2CAKugShaKbvqdfTHv9U5SonUjsP6O462s5WOpEfcsUAFasPy 13, 47 -- 4JODbg9B71WW3OzNsZKFJEE54uSnuD9h5Uxc5ZtmGn2mFMbqodIQch3NLtANuapLZHBV 13, 47 -- M0HYxpEw3N5esbul4SMFECURKWaCXF2esKwaZhKKKApRq/pyZQwGy8TlOJuGXhdz2IQA 13, 47 -- yqyg== 13, 47 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 47 -- d=1e100.net; s=20161025; 13, 47 -- h=x-gm-message-state:mime-version:in-reply-to:references:from:date 13, 47 -- :message-id:subject:to:content-transfer-encoding; 13, 47 -- bh=1guh5Wa2GBJEuLGDKB/Nj/MQijUEXpp4rJ0ZVHmFo8g=; 13, 47 -- b=csT3EmQjiwQ5bTmSbeNrhF1QqOKMSJAFSFM7oYtJMUUZZI1UQ5rHRTv4ns7w4oH12Z 13, 47 -- pNck+znOHmTMzAZyP4WVclA+sNUbZkUCwlqqWAH2sFzLSOHnIXo+B/4D+EDvhk3daRg1 13, 47 -- gzibioGUc9t3TlCjjI+d/Z2LvsXYscddkd5HT1uVk0YNLGskaRPAf4lES/uECpZnk7co 13, 47 -- mWu5Zl14fQqYu7fB7peEPVzraKvy1obomk9sO7Chcy1EVXm6VKhwFON4Oie/N3TSanhq 13, 47 -- GQhRwkJ1n9U5kY+lNTbKOzTmrh6W/2cv1hj+JEJ/n7l8LoB98b+oUhggNrhlvIAK7EOR 13, 47 -- zVcQ== 13, 47 -- X-Gm-Message-State: APf1xPDrrr6FTGilHDGWSGKhEmk5MU/rr+KTL3I1/HEnDxwnLcW20Aw8 13, 47 -- tUPBMo9YUBOWFhW5p6I/EnN6WX4PIu5THqaKXNE= 13, 47 -- X-Google-Smtp-Source: AH8x224ktEmBVqTIuSa7Q3XAUCK82HzaJjrwbHpA4668kY/I8jT+6++GKQPIiDdjaEmuXvUXdJVFMOWXm3eq9JuIL0k= 13, 47 -- X-Received: by 10.101.67.71 with SMTP id k7mr3512435pgq.136.1518216667533; 13, 47 -- Fri, 09 Feb 2018 14:51:07 -0800 (PST) 13, 47 -- MIME-Version: 1.0 13, 47 -- Received: by 10.100.138.10 with HTTP; Fri, 9 Feb 2018 14:50:46 -0800 (PST) 13, 47 -- In-Reply-To: {201802092318.w19NIgtM028746-at-microscopy.com} 13, 47 -- References: {201802092318.w19NIgtM028746-at-microscopy.com} 13, 47 -- From: Nathan McCorkle {nmz787-at-gmail.com} 13, 47 -- Date: Fri, 9 Feb 2018 14:50:46 -0800 13, 47 -- Message-ID: {CA+82U9Lsemg7dKd6dtPOykrpSNUutDqtkpc7yRF1CDSd8Nwv2g-at-mail.gmail.com} 13, 47 -- Subject: Re: [Microscopy] FW: Ask a Microscopist - Imaging board needed for a 13, 47 -- Leo 1525 13, 47 -- To: karl.hagglund-at-northwestern.edu, oshel1pe-at-cmich.edu, 13, 47 -- Microscopy-at-microscopy.com 13, 47 -- Content-Type: text/plain; charset="UTF-8" 13, 47 -- Content-Transfer-Encoding: 8bit 13, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w19NtYjt004471 ==============================End of - Headers==============================
Do you have a high vacuum gauge on the scope? With both our new Hitachi (SU3500) and our old JEOL (5600), the "ready" state comes on when the vacuum is in the high 10^-4 torr range (or even higher if there was a vacuum leak, outgassing sample, or other problem). Running a filament at such a poor vacuum will drastically reduce filament life and generally contaminate the column faster. I insist on having a gauge on any scope I run, and don't turn the beam on until the vacuum is at least mid 10^-5. With vacuum gauges under $1000, they'll quickly pay for themselves in terms of filaments and time lost changing them. I've ranted to both JEOL and Hitachi that they should make them standard on all scopes. By their own admission, half of their service calls are vacuum related, and with a gauge the end user can often find a leak and save a service visit. In our case with the closest service in Ontario, it's a $2000 flight to get here. Should be a simple budgetary calculation, but Hitachi says I'm the only customer in Canada who ever requested a vacuum gauge.
I also let the filament cool 5 to 10 minutes whenever possible before venting. I don't have any hard evidence, but it just seems logical to me that exposing a hot tungsten filament to atmosphere is going to induce more oxidation or whatever compared to a cool one.
In my experience, these precautions contribute more to filament life than slight undersaturation. Of course, you want to make sure you're not oversaturated, but I get 3-400 hours of the Hitachi filaments running in the "medium" gun setting.
Hope this helps.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Simple, clear purpose and principles give rise to complex and intelligent behavior. Complex rules and regulations give rise to simple and stupid behavior. — Dee Hock
On 2/9/2018 5:09 AM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: wschneider-at-wisc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both wschneider-at-wisc.edu as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: wschneider-at-wisc.edu Name: Bil Schneider } Organization: UW Madison } Title-Subject: [Filtered] Tungsten filaments } } Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does } anyone have any suggestions for extending filament life? We try and } operate slightly under saturated except for EBSD or EDS mapping. I’ve } heard of “seasoning” the filament but have never seen an actual protocol } or talked with anybody who’s actually done it. Any suggestions would be } appreciated! } } Login Host: 24.240.36.43 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 7, 53 -- From microscopy.listserver-at-gmail.com Fri Feb 9 03:08:08 2018 } 7, 53 -- Received: from mail-oi0-f51.google.com (mail-oi0-f51.google.com [209.85.218.51]) } 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19988gd004954 } 7, 53 -- for {microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 03:08:08 -0600 } 7, 53 -- Received: by mail-oi0-f51.google.com with SMTP id c189so5483521oib.12 } 7, 53 -- for {microscopy-at-microscopy.com} ; Fri, 09 Feb 2018 00:03:40 -0800 (PST) } 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=gmail.com; s=20161025; } 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 7, 53 -- bh=IqkG8nut+Jdti8HQSfY1x1ROkr5cfjQ7/oJEo5aM9XU=; } 7, 53 -- b=EVlvyZdIAsR8eih/9KObYEtcdFgzguDCb55CCzXShQz90Xf1Cldpy6tfwF9m/EkMsC } 7, 53 -- jGjIBNUJfDCfm9KyUQQls/kFzkF0MgUVrmB5wA3QRHQz1dYWNF2EACRS/AWO7H6MWYPt } 7, 53 -- LS9zMx+91CrrwffiZByTchBHBrRD4RUT5efDKZnLMJrClgS6ViC4XB/tZd4nb4/s7LYr } 7, 53 -- U+i+npdk2TjdeHQhxlHrlOFHdJx4I4zU60nSVs6JVMj1KeMLmNtwZA5oM2FIjMk4jDwo } 7, 53 -- lad0JucueoehwzJZCXAJWm6SN+wlKuHXWw8G4BXFjlFIdsuDBXCqWI/IAv2CDGaoqMPz } 7, 53 -- 1tgw== } 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=1e100.net; s=20161025; } 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 7, 53 -- :user-agent:mime-version:in-reply-to:content-language } 7, 53 -- :content-transfer-encoding; } 7, 53 -- bh=IqkG8nut+Jdti8HQSfY1x1ROkr5cfjQ7/oJEo5aM9XU=; } 7, 53 -- b=fD+cL9JoveCo1gY25/ItNcAhDjdwMxWjC/nN2BCljC4Yxo9qe7djtBIneYz+lcJXwY } 7, 53 -- yoIYyB7+yPrnzqjB+nMOdMpMOS861ExV4iL7giqTyacbPbhtrU5afW+kzRFM+P3QeTZM } 7, 53 -- YCD+u4yABUKD/q8bvjBvuPPoU2CCrPInYbF6m/e3zOp82riqFZOobhIUKQdYa0JHSFuC } 7, 53 -- kllnIeI9ozHP42ETXgWK1wdfxh1wt7GfnM2S4JXLspGNvqx9nNnW/xf+AMihV2dDz3VK } 7, 53 -- sGJVqxhUoiSezS6on2ZWju8Vd3QUZ3hjh6j/BMwYV6rBzng6VcajvMmy5F8A/Otibz9f } 7, 53 -- m6bg== } 7, 53 -- X-Gm-Message-State: APf1xPC5yfDA+vC7r9TgCoRlqVjSLX5y1lF9t1v0/w+RHEovBFYCfUnO } 7, 53 -- fpZxUJ3RcGAfC48i/swFjYdIjA== } 7, 53 -- X-Google-Smtp-Source: AH8x227er67ZL/nArsLNlKydzXDv1ijuT+HuppzwHg+W1JhNLm6181KarZjPxiiFdJveAgRWiYK4MQ== } 7, 53 -- X-Received: by 10.202.229.206 with SMTP id c197mr1135491oih.214.1518163419414; } 7, 53 -- Fri, 09 Feb 2018 00:03:39 -0800 (PST) } 7, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([175.103.25.178]) } 7, 53 -- by smtp.googlemail.com with ESMTPSA id q57sm918364otq.25.2018.02.09.00.03.37 } 7, 53 -- for {microscopy-at-microscopy.com} } 7, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 7, 53 -- Fri, 09 Feb 2018 00:03:38 -0800 (PST) } 7, 53 -- Subject: viaWWW:Tungsten filaments } 7, 53 -- References: {201802090157.w191vncu007124-at-microscopy.com} } 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 7, 53 -- X-Forwarded-Message-Id: {201802090157.w191vncu007124-at-microscopy.com} } 7, 53 -- Message-ID: {92f37fee-f8ae-caf6-f04a-6a84f683918c-at-gmail.com} } 7, 53 -- Date: Fri, 9 Feb 2018 18:33:38 +1030 } 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 7, 53 -- Gecko/20100101 Thunderbird/52.6.0 } 7, 53 -- MIME-Version: 1.0 } 7, 53 -- In-Reply-To: {201802090157.w191vncu007124-at-microscopy.com} } 7, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed } 7, 53 -- Content-Language: en-US } 7, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
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Email: okneill-at-umich.edu Name: Owen Neill
Organization: University of Michigan
Title-Subject: [Filtered] Symposium P06, M&M2018
Message: Dear colleagues,
Apologies for the cross-postings. The deadline for abstract submission for the Microscopy & Microanalysis 2018 Annual Meeting (Baltimore, MD, 5-9 August, 2018) is February 15th, just a week away. We hope you will consider submitting an abstract to:
Symposium P06, Applications of Integrated Electron Probe Microscopy and Microanalysis in Characterizing Natural and Synthetic Materials.
A full description of this session is included below we are looking for submissions dealing with the synthesis of different microbeam techniques for evaluating composition, bonding, valence, and density-of-state in natural and synthetic materials. Papers may be submitted via the M&M2018 website, which is linked here: https://www.microscopy.org/MandM/2018/index.cfm
We are also pleased to announce the invited speakers for this symposium: Emma Bullock, Carnegie Institution Paul Edwards, Strathclyde University Raynald Gauvin, McGill University Peter Statham, Oxford Instruments Ed Vicenzi, Museum Conservation Institute
We are very excited to hear from these experts in microanalysis, and we look forward to hearing about your work as well. See you in Baltimore!
The organizers of Symposium P06: Kat Crispin, Pennsylvania State University Colin MacRae, CSIRO Mineral Resources Owen Neill, University of Michigan
Symposium Description: Electron beam instruments have been merging with other techniques to extract information such as bonding, valence, and density-of-state, which, combined with elemental analysis, offers new insights into the structure and chemistry of materials. Researchers now have access to an analytical toolbox enabling characterization in a range of controlled environments (liquid and gas) and conditions (elevated temperatures to liquid helium). This symposium showcases synergies of new and emerging techniques, while highlighting recent advances in areas such as optical analysis using RAMAN, cathodoluminescence, soft X-ray spectrometry, and others. Papers covering some or all of these new developments and re-emerging technologies are welcome.
Topics of interest include: Multi-technique, electron-microbeam-based characterizations of natural and synthetic materials. Integration of new and existing technologies to measure material chemistry, structure, boding, valence, etc. Applications of such technologies to natural and synthetic materials.
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Email: mike.marko.em-at-gmail.com Name: MIke Marko
Organization: NY
Title-Subject: [Filtered] Opportunity to learn Cryo-EM and get paid for it!
Message: Opportunity to learn Cryo-EM and get paid for it!
Dr. Rajendra Agrawals group at Wadsworth Center in Albany, NY is looking for an electron microscopy technologist for cryo-EM data collection.
This is an opportunity for someone who knows the basics of TEM operation to learn the hottest technique in biology. You will be using high-end state-of-the-art equipment and software and will be part of an internationally competitive group that has years of experience and consistent grant funding. Our group evolved from the pioneering work -- at Wadsworth -- of Joachim Frank, who shared the 2017 Nobel prize. Other PIs at our 3DEM facility are at the forefront of cryo-EM development, so Albany can be an exciting place to be. Living expenses in Albany are moderate, and our area is an attractive place to live.
If you are interested in this opportunity, please contact Dr. Agrawal at rajendra.agrawal-at-health.ny.gov
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Email: wfschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison Geosciences
Title-Subject: [Filtered] Info on Tungsten Filament optimization
Message: Thanks to all who responded to my S3400N VPSEM tungsten filament questions.
We do try to keep users from venting for 5 minutes after turning off the HV. We probably average 50 hours per filament lately, which is what aggravated me a bit because the last box was more like 80 hours. One challenge is that some users change samples multiple times in a session. Then the next user is in variable pressure changing mag and kV, vacuum, etc..... then someone will run an EBSD map overnight at 30 kV, 100 probe current. We ask a lot from the SEM, in general its a workhorse. I did have several suggestions that seem promising: The Hitachi guy called and suggested new filaments shouldnt be turned on till Vacuum has worked for a couple of hours. He said they try and wait more like 15 minutes between HV off and venting. Several responders further emphasized gently saturating the filament over a few hours, keeping it slightly under saturated for general use, and using some gun bias to concentrate the emission area. One respondent made a great point about using a vacuum gauge on the gun and waiting till Vacuum is in the 10 to the minus 5 range. I honestly dont know what the vacuum is when the Hitachi lets you turn on the HV. Adding more spacers to push the grid cap further away from the filament tip was another suggestion. I was under the impression that how ever many spacers that each individual filament came with was the correct distance as measured at the factory to keep emission current appropriate? Otherwise I handle everything with gloves and care when changing filaments and occasionally use metal polish on the grid cap and threaded holder as well as the anode. More often Ill sonicate the grid cap and holder in methanol when the gun is open to change a filament. Thanks again for all the suggestions, Ill repost this in hopes this collection of information might be useful to others with tungsten filament questions. Cheers, Bil Schneider UW Geosciences
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Title-Subject: [Filtered] Cross section on MgO substrates
Message: Hello I need to study the quality of interface of few nanometer thin layer on MgO substrate. I 've prepared cross sections by mechanical polishing followed by ion milling usig the PIPS. I am not satisfied by the quality of HAADF STEM images, especially when I compared those images to HAADF STEM images from cross sections on SrTiO3 prepared by the wedge polishing with the multiprep from ALLIED. Did someone already succeed in performing cross section on MgO using the wedge polishing? I 've tried several time without succes. What is the angle used, the strength, the trick? Thank you for your help
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X-from: Tom Hrn {tomas.hrncir-at-tescan.com} \ Hello All, in my experience there are 3 very simple rules to extend the tungsten filament lifetime:
1. Vent the microscope by nitrogen and always exchange the sample and pump the chamber quickly. This will minimize the contamination of the vacuum chamber by water and speed up the pumping.
2. After getting HV ready signal (when the gun/chamber is pumped), wait several tens of seconds or longer before heating the filament - the filament lifetime depends on the pressure exponentially. Reaching lower 10-3 Pa in the gun is perfect to extend the filament lifetime.
3. Turn off filament heating manually and wait several minutes before venting the chamber. Like that, the filament will cool down properly. Venting the filament when it is hot decreases its lifetime significantly.
By utilizing long waiting time, the filament lifetime around 1000 hours can be obtained easily, if there is no vacuum leak in the gun. So sometimes it is a question whether to wait longer and reach very long lifetime, or wait shorter and exchange filaments more frequently (changing tungsten filament is cheap and easy). For someone and on some machines, exchanging the filament might take several minutes only so it does not make sense to wait several minutes during each sample exchange procedure. For other users/machines, exchanging the filament might be a nightmare so it would be better to wait longer and extend the filament lifetime :-). Have a nice day, Tomas
We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes.
Does anyone have recommendations for other containers we can consider? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
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Email: panagiotis.kastritis-at-embl.de Name: Dr Panagiotis Kastritis
Organization: European Molecular Biology Laboratory, Heidelberg Title-Subject: [Filtered] Electron Microscopist position in Halle, Germany
Message: Dear EM community,
I would like to draw your attention to an open position of EM facility manager at the HALOmem institute, located at the Martin Luther University of Halle-Wittenberg (MLU) in Halle, Germany. Our cryoEM facility includes the 300 kV JEOL JEM-3200FSC (equipped with field emission gun, cryo-stage, in-column energy filter and GATAN K2 direct electron detector). Funds are available to acquire another high-end cryo-electron microscope. We are searching for a staff scientist/facility manager who will oversee the operation of the cryoEM facility: assist users with training on the microscopes, perform data collection and ensure timely maintenance of the microscopes. The position is offered until August 2022 and the call closes on the 5th of March. The candidate should have an extensive experience in electron microscope operation, preferably under cryogenic conditions. The HALOmem institute (http://www.halomem.de/) is interested in membrane protein research and houses research groups with strong expertise in protein biochemistry, X-ray crystallography, NMR and proteomics. Interested candidates should apply via e-mail or request further information from Dr. Panagiotis Kastritis (pk-at-halomem.de) -- Official call (in german): http://www.verwaltung.uni-halle.de/dezern3/Ausschr/18_138.pdf English translation: http://www.halomem.de/images/uploads/EM_manager_ad-eng_inofficial.pdf Job details (in english): http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf (http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf) ---
Thank you very much, Kindest Regards,
Panos -- Dr. Panagiotis Kastritis Structural and Computational Biology Unit European Molecular Biology Laboratory Meyerhofstra?e 1, 69117 Heidelberg, Germany
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} We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes. } Does anyone have recommendations for other containers we can consider? Thanks!
I have been using this model for storage and transport of SEM samples but also whole TEM grid boxes:
They work really well, up to the point where they start questioning you at the airport security check where you'd be going with that "crystal ashtray" ;)
Guenter
-- Dr. Guenter Resch Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net Ziegelhofstrasse 136/1, 1220 Vienna, Austria - Phone +43 664 94 17 210 Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234
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if your SEM computer has still control of vacuum / high tension and scan amp, the easiest solution would be to attach an external scan system like the DISS5 from www.pointelectronic.de I am using.
Next possibility would be to exchange the complete electronics to a new one. Pointelectronic does this for some of the Zeiss / FEI / Jeol scopes. There should be an US-based agent also for their hardware.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 10.02.18 um 00:21 schrieb oshel1pe-at-cmich.edu: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } } } } } } Name:karl Hagglund } School:Northwestern University } Grade/Education Level:Graduate } Location:Evanston, IL } US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject} } Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board } Your Question: } Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts. } Thank you, } Karl Hagglund } } } } } } ==============================Original Headers============================== } 10, 59 -- From oshel1pe-at-cmich.edu Fri Feb 9 16:58:07 2018 } 10, 59 -- Received: from NAM01-BN3-obe.outbound.protection.outlook.com (mail-bn3nam01on0113.outbound.protection.outlook.com [104.47.33.113]) } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w19Mw62x009150 } 10, 59 -- for {microscopy-at-microscopy.com} ; Fri, 9 Feb 2018 16:58:07 -0600 } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 59 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 10, 59 -- bh=UKlhs70T8N2RchOoD5XEkqH/C+5CkuB8zI2H8YME/7A=; } 10, 59 -- b=OgcR5ZQlZB2cg+Lc6nMDHbX6DkhW7M4aONwtOxdVLewFCgsiaHth+fj0YCWJArD0hez8aScoYPyEYAu/KQoV5ghtez33mhoajIdyA2HS8ebvmJ8L6qqXhifJsk3JV6qes9Q6xcUuKxbS84wscL0olahXsoPYxBx/pYrk9x57rW0= } 10, 59 -- Received: from CY1PR0501MB1676.namprd05.prod.outlook.com (10.163.140.15) by } 10, 59 -- CY1PR0501MB1386.namprd05.prod.outlook.com (10.160.148.140) with Microsoft } 10, 59 -- SMTP Server (version=TLS1_2, } 10, 59 -- cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 15.20.485.3; 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==============================Original Headers============================== 10, 30 -- From diller-at-stefan-diller.com Tue Feb 13 04:18:23 2018 10, 30 -- Received: from mailout020.rox.net (mailout020.rox.net [212.63.85.220]) 10, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1DAINW7013657 10, 30 -- for {microscopy-at-microscopy.com} ; Tue, 13 Feb 2018 04:18:23 -0600 10, 30 -- Received: from localhost ([127.0.0.1]) 10, 30 -- by mailout02.rox.net with esmtp (Exim 4.80) 10, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 10, 30 -- id 1elWf9-000Dpw-4T; Tue, 13 Feb 2018 10:14:07 +0100 10, 30 -- Received: from pd9f9d614.dip0.t-ipconnect.de ([217.249.214.20] helo=mac-pro.local) 10, 30 -- by mailout02.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 10, 30 -- (Exim 4.80) 10, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 10, 30 -- id 1elWf8-000Dpg-W3; Tue, 13 Feb 2018 10:14:07 +0100 10, 30 -- Subject: Re: [Microscopy] FW: Ask a Microscopist - Imaging board needed for a 10, 30 -- Leo 1525 10, 30 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 10, 30 -- karl.hagglund-at-Northwestern.edu 10, 30 -- References: {201802092321.w19NLhWW032075-at-microscopy.com} 10, 30 -- From: stefan diller {diller-at-stefan-diller.com} 10, 30 -- Message-ID: {d476038a-215e-5276-facb-707aed647904-at-stefan-diller.com} 10, 30 -- Date: Tue, 13 Feb 2018 10:14:05 +0100 10, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:52.0) 10, 30 -- Gecko/20100101 Thunderbird/52.6.0 10, 30 -- MIME-Version: 1.0 10, 30 -- In-Reply-To: {201802092321.w19NLhWW032075-at-microscopy.com} 10, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 30 -- Content-Transfer-Encoding: 7bit 10, 30 -- Content-Language: en-GB 10, 30 -- X-Envelope-From: {diller-at-stefan-diller.com} 10, 30 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
I have just picked up this offering and with 50 years EM experience I fully agree with Tomas' posting. This procedure should be in your s.o.p. for running a scanning electron microscope, any other way is just not good enough!
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 12 February 2018 21:15 To: protrain-at-emcourses.com
X-from: Tom Hrn {tomas.hrncir-at-tescan.com} \ Hello All, in my experience there are 3 very simple rules to extend the tungsten filament lifetime:
1. Vent the microscope by nitrogen and always exchange the sample and pump the chamber quickly. This will minimize the contamination of the vacuum chamber by water and speed up the pumping.
2. After getting HV ready signal (when the gun/chamber is pumped), wait several tens of seconds or longer before heating the filament - the filament lifetime depends on the pressure exponentially. Reaching lower 10-3 Pa in the gun is perfect to extend the filament lifetime.
3. Turn off filament heating manually and wait several minutes before venting the chamber. Like that, the filament will cool down properly. Venting the filament when it is hot decreases its lifetime significantly.
By utilizing long waiting time, the filament lifetime around 1000 hours can be obtained easily, if there is no vacuum leak in the gun. So sometimes it is a question whether to wait longer and reach very long lifetime, or wait shorter and exchange filaments more frequently (changing tungsten filament is cheap and easy). For someone and on some machines, exchanging the filament might take several minutes only so it does not make sense to wait several minutes during each sample exchange procedure. For other users/machines, exchanging the filament might be a nightmare so it would be better to wait longer and extend the filament lifetime :-). Have a nice day, Tomas
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Title-Subject: [Filtered] Career Opportunity - Electron Microscopy Technologist
Message: Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource. The individual must have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
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Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] GWNIC to host afternoon of high pressure freezing lectures
Message: Hi all,
The George Washington University Nanofabrication and Imaging Center would like to invite you to an afternoon of high pressure freezing lectures on Monday March 5, 2018. So if you are in Washington DC, just drop around and spend an inspiring afternoon with some local experts in the field of high pressure freezing.
https://nic.gwu.edu/high-pressure-freezing
Chris Brantner
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Email: tina.williams-at-ars.usda.gov Name: Tina Williams
Organization: USDA
Title-Subject: [Filtered] old style FEI camera
Message: Hello,
We have an old style FEI camera film cassette holder that fits an FEI Technai 12. If anyone is interested please contact me offline.
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I want to thank everyone who responded to my TEM question and I regret things are so crazy I can't respond to each person. The information provided was very useful and I plan to remove and clean the Wehneit cup and reinstall. That should, I hope resolve my problems.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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Title-Subject: [Filtered] Job Opening - NASA Johnson Space Center - SEM/EBSD
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Email: rvancamp-at-kettering.edu Name: Rick
Organization: Kettering University
Title-Subject: [Filtered] JEM-2100 Plus Parts Need - Virtually Urgent
Message: Hello Everyone,
I am the microscopist at Kettering University. We own a JEM-2100 Plus TEM. I recently observed that the Wehnhelt and the cathode mounting base the Wehnhelt screws onto have experienced damage to their mating threads. This precludes these parts from being used. I am posting this message to the community in an attempt to identify parts we can obtain from those that have these two parts in addition to the ability to loan them to us on a temporary basis or sell them to us.
The JEM-2100 Plus features a Wehnelt and cathode mount that are common to many Jeol instruments yet, I do not have a complete list. We must consider consulting Jeol to ensure compatibility before any transaction occurs. Note this is the Jeol K-type cathode mount. For example, our TEM uses the Jeol MA113008 (03) tungsten filament, the Denka M3 LKSH 60-degree sharp tip LaB6 cathode, and the APTech mini-Vogel mount 15927 LaB6 cathode. These cathodes are provided as a reference only. I do not know the complete list of LaB6 cathodes that are compatible with our JEM-2100 Plus.
Please contact me if you have additional questions or think you are in a position to help us. Thank you for your consideration.
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Hello, I am teaching a TEM class and I am having trouble finding reliable, readable mappings of the Kikuchi lines for diamond Si. There seems to be a couple of hand-drawn ones floating around, but they are very low-res. I was wondering if anyone had one they could share, or direct me to a place where they can be simulated?
Thank you!
==============================Original Headers============================== 2, 49 -- From klappar.panova-at-gmail.com Wed Feb 14 15:25:19 2018 2, 49 -- Received: from mail-pl0-f48.google.com (mail-pl0-f48.google.com [209.85.160.48]) 2, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1ELPJOf019904 2, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Feb 2018 15:25:19 -0600 2, 49 -- Received: by mail-pl0-f48.google.com with SMTP id bd10so4321596plb.1 2, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Feb 2018 12:21:09 -0800 (PST) 2, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 49 -- d=gmail.com; s=20161025; 2, 49 -- h=from:content-transfer-encoding:mime-version:subject:message-id:date 2, 49 -- :to; 2, 49 -- bh=89huRmq9vMtZbuC0+cev7qPL+JW8QObtv5fcb/bbnMs=; 2, 49 -- b=YevWMpCF7AEZ+RJbHC8mByfMH4isHHyrRPfivPHQPB/8aLQuQaYw29WZAjh3X3Xl6d 2, 49 -- Vg1uVy58lZjBruLbUIk/WNsm6/FwWIANnyDGRgTIvn3OM3dQlQ2JsYqvgZGhhkK0+FLU 2, 49 -- vpghCzx193e9+kPEpX3dhLhFH3/imyH7Z1bRYBQ2aFDawLV/EMp+074Ro8IY9NbyXEHl 2, 49 -- QHWJczOJFaTuDfeJzs5m49yOCMTbjFJPY61B7uOKapbm2GVp27wMoWsxEdZX4CErUcGA 2, 49 -- T6bSjCpKvWRjgP9bJrrglVhLX5UtqmZxjWuIrMy5gVeOHNO2UHoYOL6gs3yGqZCBB8XU 2, 49 -- FcRQ== 2, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 49 -- d=1e100.net; s=20161025; 2, 49 -- h=x-gm-message-state:from:content-transfer-encoding:mime-version 2, 49 -- :subject:message-id:date:to; 2, 49 -- bh=89huRmq9vMtZbuC0+cev7qPL+JW8QObtv5fcb/bbnMs=; 2, 49 -- b=gitLKQVLNqw8T3XVPs++nmo1DxueqnPSLtSdSN6sWX0g8cTtCZF6jETmHRlVX/QteM 2, 49 -- DhNEH8bKiaci7bpCQoxOqYrk8rJTHRZbD4GAprUxo0eXGnMOjB1t3U82lAdLQA++qOEo 2, 49 -- bG0EZSE91sGltC9nBJ+2sig//5ORmVTxAnOIfICxkESIFKBYYv072+JJrTV+wnqKHoZx 2, 49 -- KxHJy3+AVkEGHCKmHNSZhe2kD/jmg94KcaJxhLPctvDBVCdDjonjpMvwQ8cxUGUyK4aw 2, 49 -- 0yx7s3QDddbuYVDWgRKnb8t4xPuH1m4HdBSPWXnQC+Cp0FiKXolpDV+Fm0i5vllbjhbc 2, 49 -- Deew== 2, 49 -- X-Gm-Message-State: APf1xPDG7Q4bc2BvX/OlTUJXLIuEit4a8XUkzruN6pP80vytwXugE3Kj 2, 49 -- 0+ZCkpBrt5PLT1v29yfKg5xt//2P 2, 49 -- X-Google-Smtp-Source: AH8x224M6n7KSOENkgneFAj6PkmeKw1KEkSqAKGCWNFKvnPvBqE4oRzs2WdVunt8/qEByrkB6j7JJQ== 2, 49 -- X-Received: by 2002:a17:902:8a89:: with SMTP id p9-v6mr189796plo.397.1518639668368; 2, 49 -- Wed, 14 Feb 2018 12:21:08 -0800 (PST) 2, 49 -- Received: from [192.168.11.11] (c-73-70-192-182.hsd1.ca.comcast.net. [73.70.192.182]) 2, 49 -- by smtp.gmail.com with ESMTPSA id c185sm40883137pfb.146.2018.02.14.12.21.06 2, 49 -- for {Microscopy-at-microscopy.com} 2, 49 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 2, 49 -- Wed, 14 Feb 2018 12:21:07 -0800 (PST) 2, 49 -- From: Ouliana Panova {klappar.panova-at-gmail.com} 2, 49 -- Content-Type: text/plain; 2, 49 -- charset=us-ascii 2, 49 -- Mime-Version: 1.0 (Mac OS X Mail 11.2 \(3445.5.20\)) 2, 49 -- Subject: Kikuchi maps 2, 49 -- Message-Id: {D16A7B71-3F9D-4613-9B2A-F41FF8886EDD-at-gmail.com} 2, 49 -- Date: Wed, 14 Feb 2018 12:21:06 -0800 2, 49 -- To: Microscopy-at-microscopy.com 2, 49 -- X-Mailer: Apple Mail (2.3445.5.20) 2, 49 -- Content-Transfer-Encoding: 8bit 2, 49 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w1ELPJOf019904 ==============================End of - Headers==============================
I recently got a second hand Noran Quest EDX system which I currently can't power on since the detector temperature is too high. I let it cool down for 24 hours with a fully filled up dewar. I believe that the measurement of the "detector temp" is wrong since there is no voltage (0.00 Volts) measured at all. Also, there is no voltage difference between a warm detector and a detector that has cooled down for 24 hours. I am thus looking for technical information including schematics. I suspect that there could be a contact issue or a broken cable.
I started the Noran Quest "sys_status" tool. It gives me the following output:
As you can see, the "detector temp" is 0.00 Volts.
- What are normal voltage levels for the temperature measurements ? (If the LN sensor voltage is 4.28 Volts, how does that translate to °C ? Is there a formula to translate the voltage to temperature? Is 4.28 Volts OK for a filled up dewar ?)
- What voltage should I get for the "detector temp" ? (What voltage should be measured if the detector is warm ? What voltage should be there if the detector is cooled down ?)
- Where is the temperature sensor physically located within the detector ?
- What kind of temperature sensor is it (resistor with X Ohms) ?
- At the detector there is a d-sub connector. What is the pinout of this connector and where is the "detector temp" output ?
Any technical information to get my detector working would be really appreciated.
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Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan
Organization: The University of Adelaide, Adelaide Microscopy
Title-Subject: [Filtered] ancient grain
Message: Hi all, I have been tasked with imaging an ancient grain. It is 1000 year old millet and I have one only! I have done SEM/TEM of grain before but not one so old. I am thinking SEM may be the way to go. The investigators would like to see the internal structure of the grain (if any) and I have no idea whether it will be 'normal', caramelised or powder inside! It must be fixed in order to be released from quarantine so my first question is should I use an aqueous fixative or alcohol? Any other advice would be gratefully accepted!
Cheers Lisa
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Email: btracy-at-eag.com Name: Bryan Tracy
Organization: EAG Laboratories
Title-Subject: [Filtered] cutting the slab?
Message: Hello Everyone,
I know this is a subject on which reasonable people can disagree, but I wanted to ask what has been your experience with cutting the slab to reduce low frequency vibrations?
The floor appears to be moving in the horizontal plane at 1.6Hz (4.4 reduction factor needed) and in the vertical direction at 5HZ. (3.1 reduction factor needed)
much appreciated
bryan
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Lisa,
It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix much anyway. If alcohol is good enough to release from quarantine, use 70 or 80% ethanol, then go through to 100% EtOH. Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate at 20 deg C.
After the 2nd or 3rd 100% EtOH, you could put the grain in liquid nitrogen and hit it with a razor blade, maybe gently crush it. You’ll get a brittle fracture that will expose the starch grains. This part will be particularly fun (or “fun”) if your grain is tiny ...
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan
Organization: The University of Adelaide, Adelaide Microscopy
Title-Subject: [Filtered] ancient grain
Message: Hi all, I have been tasked with imaging an ancient grain. It is 1000 year old millet and I have one only! I have done SEM/TEM of grain before but not one so old. I am thinking SEM may be the way to go. The investigators would like to see the internal structure of the grain (if any) and I have no idea whether it will be 'normal', caramelised or powder inside! It must be fixed in order to be released from quarantine so my first question is should I use an aqueous fixative or alcohol? Any other advice would be gratefully accepted!
Do you know if the vibration is resulting form sources inside the building or the from the general area outside? EAG is in between a number of freeways there in Sunnyvale and the 101 isn't far away so if it is the underlying ground below slab, you might make it worse cutting the slab. I'm sure some of the experts on here have experience doing this and will advise.
You might also check with Vibration Engineering Consultants - www.vibeng.com {http://www.vibeng.com} - in Pacifica, CA. This is their specialty.
I would also be remiss if I didn't suggest that you consider a Vibration Isolation Base Platform. The active type we offer suppresses vibration starting at 0.7 Hz and has 90% suppression by 2Hz. I would avoid a passive type isolator as most of them have a resonant frequency right around 2 to 4 Hz which would make things even worse for the vibrations you have. Likewise if you decide to cut the slab, careful if you use an elastomeric sealant and check it's resonant frequency.
Have a look here at our line of Vibration Isolation Platforms for SEM/TEM/FIB: http://www.elementpi.com/active-vibration-isolation-platform-products/
Good Luck,
Mike Toalson element Pi, LLC 833-314-1593
On Fri, Feb 16, 2018 at 2:15 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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I know this is a subject on which reasonable people can disagree, but I wanted to ask what has been your experience with cutting the slab to reduce low frequency vibrations?
The floor appears to be moving in the horizontal plane at 1.6Hz (4.4 reduction factor needed) and in the vertical direction at 5HZ. (3.1 reduction factor needed)
much appreciated
bryan
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See below for a request from a paleobiologist here at the National Museum of Natural History. If you have a modern image of collagen fibrils you are willing to share in some manner he would be interested in hearing from you directly. His contact is GreenwaltD-at-si.edu.
Thanks,
Scott Whittaker Lab Manager Imaging P.O. Box 37012 MRC-104 Room W150 Washington, DC 20013-7012 w 202.633.0891 mailto:whittaks-at-si.edu
SMITHSONIAN INSTITUTION NATIONAL MUSEUM OF NATURAL HISTORY https://www.facebook.com/nmnh.fanpage/ | https://twitter.com/NMNH | https://www.instagram.com/smithsoniannmnh/
X-from: "Greenwalt, Dale" {GreenwaltD-at-si.edu}
Hi Lisa,
Fixation in 95-100% methanol or ethanol may be better for your grain - there will be less tissue swelling. The dry grain will contain only about 8-10% water, so going into 100% solvent will be fine. A little water (i.e. 95% solvent) may help penetration. Methanol will penetrate a little better, but it will take some time for any solvent to get deep into a dry grain.
Another option after drying would be high-resolution (+/- phase contrast) x-ray CT - it would quickly show you if the grain had any contents, and give you an idea of what they are without breaking the seed. There are at least a couple of labs I know of in Australia that do this, one of them is here in Canberra at the ANU - https://ctlab.anu.edu.au/, and since your seed will be dead after going through solvent, you don't have to worry about the high kV x-rays killing the tissue. Millet seed is pretty small, so you'd get good resolution of the innards.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia Adjunct Prof, EH Graham Centre, CSU & Research School of Biology, ANU
T 61 2 6246 5475 E rosemary.white-at-csiro.au
On 17/2/18, 7:29 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Lisa,
It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix much anyway. If alcohol is good enough to release from quarantine, use 70 or 80% ethanol, then go through to 100% EtOH. Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate at 20 deg C.
After the 2nd or 3rd 100% EtOH, you could put the grain in liquid nitrogen and hit it with a razor blade, maybe gently crush it. You’ll get a brittle fracture that will expose the starch grains. This part will be particularly fun (or “fun”) if your grain is tiny ...
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan
Organization: The University of Adelaide, Adelaide Microscopy
Title-Subject: [Filtered] ancient grain
Message: Hi all, I have been tasked with imaging an ancient grain. It is 1000 year old millet and I have one only! I have done SEM/TEM of grain before but not one so old. I am thinking SEM may be the way to go. The investigators would like to see the internal structure of the grain (if any) and I have no idea whether it will be 'normal', caramelised or powder inside! It must be fixed in order to be released from quarantine so my first question is should I use an aqueous fixative or alcohol? Any other advice would be gratefully accepted!
X-from: Henk Colijn {colijn.1-at-osu.edu} Reply-To: Henk Colijn {colijn.1-at-osu.edu} To: microscopy.listserver-at-gmail.com
Hi Bryan,
Those are very low frequencies. They should be in a range that an active compensation system should be able to handle it.
Another thing to consider is the resonant frequency of the slab (even though it is damped by the ground). The more mass in the slab, the lower its resonant frequency. The install guides for our microscopes show that the microscope is much more sensitive at very low frequencies. The allowable vibration at 10Hz is approx 10x lower than at 20Hz. Since too much mass can push the slab resonance down into the range where the microscope is more sensitive you may want to estimate the resonant frequency of your current slab and then consider whether to slice it or not. It would also be useful to measure the ground vibration away from the instrument to get an idea of the driving frequencies.
Good luck! Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 2/16/2018 4:45:19 AM
X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}
Thanks Phil. I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking this grain might have a more detrimental effect. Thanks for your advice
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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } } } Lisa, } } It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix } much anyway. If alcohol is good enough to release from quarantine, use } 70 or 80% ethanol, then go through to 100% EtOH. } Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate } at 20 deg C. } } After the 2nd or 3rd 100% EtOH, you could put the grain in liquid } nitrogen and hit it with a razor blade, maybe gently crush it. You’ll } get a brittle fracture that will expose the starch grains. } This part will be particularly fun (or “fun”) if your grain is tiny ... } } Phil } ------------- } Philip Oshel } Imaging Facility Director } Biology Department } 1304 Biosciences } 1455 Calumet Ct. } Central Michigan University } Mt. Pleasant, MI 48859 } 989 774-3576 office } 989 774-7567 lab } } } } } } Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan } } Organization: The University of Adelaide, Adelaide Microscopy } } Title-Subject: [Filtered] ancient grain } } Message: Hi all, } I have been tasked with imaging an ancient grain. It is 1000 year old } millet and I have one only! I have done SEM/TEM of grain before but not } one so old. I am thinking SEM may be the way to go. The investigators } would like to see the internal structure of the grain (if any) and I } have no idea whether it will be 'normal', caramelised or powder inside! } It must be fixed in order to be released from quarantine so my first } question is should I use an aqueous fixative or alcohol? } Any other advice would be gratefully accepted! } } Cheers } Lisa } } } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Fri Feb 16 } 14:19:28 2018 } 16, 53 -- Received: from mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} } (mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} [209.85.160.46]) } 16, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } w1GKJS9B005923 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb 2018 } 14:19:28 -0600 } 16, 53 -- Received: by mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} with SMTP id } p5so2166846plo.12 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb } 2018 11:15:24 -0800 (PST) } 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=gmail.com {http://gmail.com} ; s=20161025; } 16, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 16, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=GE5+xXLFrelnACEZ+qb9LrabEgUCAn6Lq2squbWTpQikdFp75iK6bIxh2zFnC3KhiR } 16, 53 -- +zjgqcMwRwH+GTq2JSAgvTFfMB55JJgUzYYk7dd8i1L4pBeh38LWDDvF/4zFBt95T1UT } 16, 53 -- 8WAPsoPlzS3aoVcpxiCJ+JClDJCZXyiE0alPAWG4ZnWHyttpCgqegVibj4GjOsnbtDSq } 16, 53 -- yw7JMRvrh79fb+vXqwS0HhRoH/kzVFPiW6ar9kkagZ26AZ7QLDQBrmLm7AAsUh8HfcF6 } 16, 53 -- s+T91z7j8+6LXqNUegeYgPD8N30jfYPaBj36TCyY7mLD+uwNqARcwvcDpO5toDesl/6i } 16, 53 -- /NmA== } 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=1e100.net {http://1e100.net} ; s=20161025; } 16, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 16, 53 -- :user-agent:mime-version:in-reply-to:content-language } 16, 53 -- :content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=QdtuGp/nzDKOePj1apmNn88W2CmkUDhmqMhywXO7YH/DuCNMx3db1YHX2Urhqjylb/ } 16, 53 -- LPJYbLPHmaHc4+6erBsfifIVqKUMHEjzbU1e9dJ0ilSliShV6btDfGvqvSvl/ToZTfFn } 16, 53 -- DIJQC3eId0NYkyMR97fUoPoLdRvGmARecoAZaYmBsXwGDRLe0Vf3fqCRfQxYlsMAoWCH } 16, 53 -- rj3sULHYi2psR79LQsIPExm2Ej8vSkxI7WXRkGis5cG/GfWxJSIc8bYAOg2VC1MB+DNN } 16, 53 -- gb9HwnikuHtmO7xae337nSnI9bzeA/cn3uuSznrPP3TMu2fQ4loQYuntT98yCZnREGHG } 16, 53 -- Gu4A== } 16, 53 -- X-Gm-Message-State: APf1xPBfFpAudrNxqfQxQRI9reKSggIkGQjJWygh9oB9SNA/mNBcECwm } 16, 53 -- /VgMY+RDCuEltUcH5bksSPdvRQ== } 16, 53 -- X-Google-Smtp-Source: } AH8x227HuGE720DzgnhClPqS9EKAAiKPspMA1UrZJhOFXu32me+/xf87MQiz5g5QIyjCNv44s/yjbw== } 16, 53 -- X-Received: by 2002:a17:902:34e:: with SMTP id 72-v6mr6775268pld.277.1518808523341; } 16, 53 -- Fri, 16 Feb 2018 11:15:23 -0800 (PST) } 16, 53 -- Received: from NestorsnOSX1163.lan (220-244-30-118.static.tpgi.com.au } {http://220-244-30-118.static.tpgi.com.au} . 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I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves faculty and undergraduate student users for research projects, and trains undergraduates via courses. After training, users carry out their own specimen preps.
Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
I would appreciate any comments from folks who have experience with either of these, as well as suggestions about other models I may have overlooked but should consider.
Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} .
/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ *Please consider the environment before printing this e-mail*
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
We have the Tousimis 815B & 915B. We process a lot of samples of varying sizes (some quite large) & they do an excellent job & are easy/straightforward to use. We have an advantage since we're pretty much next door to the company, but they are very helpful. I haven't used the EMS.
Deborah
Sent from my iPhone
} On Feb 18, 2018, at 11:16 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Factor, Jan {Jan.Factor-at-purchase.edu} } } } } Dear List: } } I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves } faculty and undergraduate student users for research projects, and trains undergraduates via } courses. After training, users carry out their own specimen preps. } } Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931. } } I would appreciate any comments from folks who have experience with either of these, as well as } suggestions about other models I may have overlooked but should consider. } } Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu } {mailto:jan.factor-at-purchase.edu} . } } --Many thanks, *Jan * } } Jan Robert Factor, Ph.D. } } Professor of Biology } } Purchase College, State University of New York } } Purchase, NY 10577 } } Office: 2016 Natural Science Bldg., 914-251-6659 } } Office Hours (Spring 2018): } } Tues., 11:00-12:00, and Thurs., 1:30-2:30 } } jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} } } /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* } {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ } *Please consider the environment before printing this e-mail* } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com Sun Feb 18 10:53:43 2018 } 19, 53 -- Received: from mail-it0-f43.google.com (mail-it0-f43.google.com [209.85.214.43]) } 19, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1IGrhv0021068 } 19, 53 -- for {microscopy-at-microscopy.com} ; Sun, 18 Feb 2018 10:53:43 -0600 } 19, 53 -- Received: by mail-it0-f43.google.com with SMTP id o13so6661083ito.2 } 19, 53 -- for {microscopy-at-microscopy.com} ; Sun, 18 Feb 2018 07:49:45 -0800 (PST) } 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=gmail.com; s=20161025; } 19, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 19, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 19, 53 -- bh=se/2HzDj1HUWS5xYRiWI93RZvYwNKqpezKNC3pJ1kYY=; } 19, 53 -- b=LxEpN93LCGD0YU3GetB+jq48DFBz+9v+Z9kV3gp6GnrxsVuJJVHkSQsMAP6i7shQOc } 19, 53 -- YzBkw24axX4c9TI/3Koq0EyrAtnXAoczlONbWMUyDDLes95j0MdbKzFtReRtpKZv7iG1 } 19, 53 -- G5kIR0BcScrU0MvxbTnMKIuglZVpu4Bv6uM2mONKYQWVXOZyF2BmUGywic9kwjFnl/Wv } 19, 53 -- CAdcFraHh+1KaYvY+cDw11WzRP+cDkAbredNwx9Hz3vLw+FUl6H1PdYZmYl9TIpw/q7t } 19, 53 -- MyrScHJD/4ulFNg937MKA5eQ70ghzVmCCVB1PnpReBQ9yjBC0yCCaCmMF5T15A15OzZx } 19, 53 -- DpSg== } 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=1e100.net; s=20161025; } 19, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 19, 53 -- :user-agent:mime-version:in-reply-to:content-language } 19, 53 -- :content-transfer-encoding; } 19, 53 -- bh=se/2HzDj1HUWS5xYRiWI93RZvYwNKqpezKNC3pJ1kYY=; } 19, 53 -- b=KnRgyqB37C0aSVFASW+g4cybGgLsXbmheoNnDRXmgMsbue+g3zmcN9JzmqgpJEevus } 19, 53 -- hSB4nG/mfQJXOol3WO8ddF7X0QB7ZGuiVsstomuQLlKL7PO4oHY2zX4hFcL53KL7Lzyt } 19, 53 -- ERCPnJka5wbekD8IO5fHnzeo08ZO+c7hMrNIJ9dDcfFX+mGqEfRyWVyy4FWOGgRf374L } 19, 53 -- jWeq4GhJbDDdlba4Hfslw4hofe240DuDLrwKqXN0RiECclVkfBar0OBcV4JERep5edd2 } 19, 53 -- izMWLhSr9SgxWKnbAuuv3WqnXt2pcqb4mIb8NAKC1a2f8J8dnsl7W3/5jVF4oBS0FWs4 } 19, 53 -- ++2Q== } 19, 53 -- X-Gm-Message-State: APf1xPC45Onm+IuNvfy15EGfclpWyyhmAsGDeCbBSiYolidDCXUL7KOz } 19, 53 -- EU/Tki43CNruzcogrf+W/WUz3iwp } 19, 53 -- X-Google-Smtp-Source: AH8x226QMHZW/VYa84ITElkJvhANGmaV8diteKaAVurUg7n/r2kSuDOjf5H/g3i+09GX3OwcFQqMiQ== } 19, 53 -- X-Received: by 10.36.188.196 with SMTP id n187mr16678372ite.27.1518968984734; } 19, 53 -- Sun, 18 Feb 2018 07:49:44 -0800 (PST) } 19, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:9915:6b6a:bf8d:aa6e]) } 19, 53 -- by smtp.googlemail.com with ESMTPSA id s24sm6136616ioa.34.2018.02.18.07.49.44 } 19, 53 -- for {microscopy-at-microscopy.com} } 19, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 19, 53 -- Sun, 18 Feb 2018 07:49:44 -0800 (PST) } 19, 53 -- Subject: Fwd: Critical point dryer suggestions } 19, 53 -- References: {cf1e8ba6aa794cca959debc5b98c33ef-at-VSMAILMB02.purchase.edu} } 19, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 19, 53 -- X-Forwarded-Message-Id: {cf1e8ba6aa794cca959debc5b98c33ef-at-VSMAILMB02.purchase.edu} } 19, 53 -- Message-ID: {ffb0d64a-5249-79ae-6249-132258e20caa-at-gmail.com} } 19, 53 -- Date: Sun, 18 Feb 2018 09:49:43 -0600 } 19, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 19, 53 -- Gecko/20100101 Thunderbird/52.5.2 } 19, 53 -- MIME-Version: 1.0 } 19, 53 -- In-Reply-To: {cf1e8ba6aa794cca959debc5b98c33ef-at-VSMAILMB02.purchase.edu} } 19, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 19, 53 -- Content-Language: en-US } 19, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Lisa,
Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve used it for corn and barley, but if you do have millet … oof.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}
Thanks Phil. I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking this grain might have a more detrimental effect. Thanks for your advice
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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
} } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } } } Lisa, } } It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix } much anyway. If alcohol is good enough to release from quarantine, use } 70 or 80% ethanol, then go through to 100% EtOH. } Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate } at 20 deg C. } } After the 2nd or 3rd 100% EtOH, you could put the grain in liquid } nitrogen and hit it with a razor blade, maybe gently crush it. You’ll } get a brittle fracture that will expose the starch grains. } This part will be particularly fun (or “fun”) if your grain is tiny ... } } Phil } ------------- } Philip Oshel } Imaging Facility Director } Biology Department } 1304 Biosciences } 1455 Calumet Ct. } Central Michigan University } Mt. Pleasant, MI 48859 } 989 774-3576 office } 989 774-7567 lab } } } } } } Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan } } Organization: The University of Adelaide, Adelaide Microscopy } } Title-Subject: [Filtered] ancient grain } } Message: Hi all, } I have been tasked with imaging an ancient grain. It is 1000 year old } millet and I have one only! I have done SEM/TEM of grain before but not } one so old. I am thinking SEM may be the way to go. The investigators } would like to see the internal structure of the grain (if any) and I } have no idea whether it will be 'normal', caramelised or powder inside! } It must be fixed in order to be released from quarantine so my first } question is should I use an aqueous fixative or alcohol? } Any other advice would be gratefully accepted! } } Cheers } Lisa } } } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Fri Feb 16 } 14:19:28 2018 } 16, 53 -- Received: from mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} } (mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} [209.85.160.46]) } 16, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } w1GKJS9B005923 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb 2018 } 14:19:28 -0600 } 16, 53 -- Received: by mail-pl0-f46.google.com {http://mail-pl0-f46.google.com} with SMTP id } p5so2166846plo.12 } 16, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Fri, 16 Feb } 2018 11:15:24 -0800 (PST) } 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=gmail.com {http://gmail.com} ; s=20161025; } 16, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 16, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=GE5+xXLFrelnACEZ+qb9LrabEgUCAn6Lq2squbWTpQikdFp75iK6bIxh2zFnC3KhiR } 16, 53 -- +zjgqcMwRwH+GTq2JSAgvTFfMB55JJgUzYYk7dd8i1L4pBeh38LWDDvF/4zFBt95T1UT } 16, 53 -- 8WAPsoPlzS3aoVcpxiCJ+JClDJCZXyiE0alPAWG4ZnWHyttpCgqegVibj4GjOsnbtDSq } 16, 53 -- yw7JMRvrh79fb+vXqwS0HhRoH/kzVFPiW6ar9kkagZ26AZ7QLDQBrmLm7AAsUh8HfcF6 } 16, 53 -- s+T91z7j8+6LXqNUegeYgPD8N30jfYPaBj36TCyY7mLD+uwNqARcwvcDpO5toDesl/6i } 16, 53 -- /NmA== } 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 16, 53 -- d=1e100.net {http://1e100.net} ; s=20161025; } 16, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 16, 53 -- :user-agent:mime-version:in-reply-to:content-language } 16, 53 -- :content-transfer-encoding; } 16, 53 -- bh=NBGWJ+Ii7A39wsCa0/ly9zp+iQw8nF2l9NC7qHai2To=; } 16, 53 -- b=QdtuGp/nzDKOePj1apmNn88W2CmkUDhmqMhywXO7YH/DuCNMx3db1YHX2Urhqjylb/ } 16, 53 -- LPJYbLPHmaHc4+6erBsfifIVqKUMHEjzbU1e9dJ0ilSliShV6btDfGvqvSvl/ToZTfFn } 16, 53 -- DIJQC3eId0NYkyMR97fUoPoLdRvGmARecoAZaYmBsXwGDRLe0Vf3fqCRfQxYlsMAoWCH } 16, 53 -- rj3sULHYi2psR79LQsIPExm2Ej8vSkxI7WXRkGis5cG/GfWxJSIc8bYAOg2VC1MB+DNN } 16, 53 -- gb9HwnikuHtmO7xae337nSnI9bzeA/cn3uuSznrPP3TMu2fQ4loQYuntT98yCZnREGHG } 16, 53 -- Gu4A== } 16, 53 -- X-Gm-Message-State: APf1xPBfFpAudrNxqfQxQRI9reKSggIkGQjJWygh9oB9SNA/mNBcECwm } 16, 53 -- /VgMY+RDCuEltUcH5bksSPdvRQ== } 16, 53 -- X-Google-Smtp-Source: } AH8x227HuGE720DzgnhClPqS9EKAAiKPspMA1UrZJhOFXu32me+/xf87MQiz5g5QIyjCNv44s/yjbw== } 16, 53 -- X-Received: by 2002:a17:902:34e:: with SMTP id 72-v6mr6775268pld.277.1518808523341; } 16, 53 -- Fri, 16 Feb 2018 11:15:23 -0800 (PST) } 16, 53 -- Received: from NestorsnOSX1163.lan (220-244-30-118.static.tpgi.com.au } {http://220-244-30-118.static.tpgi.com.au} . 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22, 53 -- Sun, 18 Feb 2018 07:49:12 -0800 (PST) 22, 53 -- Subject: Fwd: Re: [Microscopy] viaWWW: ancient grain imaging help needed 22, 53 -- References: {103037D3-11ED-4A61-9E2A-62575DC02E90-at-adelaide.edu.au} 22, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 22, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 22, 53 -- X-Forwarded-Message-Id: {103037D3-11ED-4A61-9E2A-62575DC02E90-at-adelaide.edu.au} 22, 53 -- Message-ID: {aec0a42b-72ad-090e-68aa-cc90c22a2cce-at-gmail.com} 22, 53 -- Date: Sun, 18 Feb 2018 09:49:12 -0600 22, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 22, 53 -- Gecko/20100101 Thunderbird/52.5.2 22, 53 -- MIME-Version: 1.0 22, 53 -- In-Reply-To: {103037D3-11ED-4A61-9E2A-62575DC02E90-at-adelaide.edu.au} 22, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed 22, 53 -- Content-Language: en-US 22, 53 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Jan,
Tousimis is good, I’ve used those a lot. Not the Autosamdri, but the previous model. Have you considered the Polaron “bomb”? The larger sample chamber (in the regular size) is very handy. SPI sells this as a rebranded product. I use it in a multi-user facility that teaches undergrads and grad students.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves faculty and undergraduate student users for research projects, and trains undergraduates via courses. After training, users carry out their own specimen preps.
Two models I’ve come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
I would appreciate any comments from folks who have experience with either of these, as well as suggestions about other models I may have overlooked but should consider.
Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} .
/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ *Please consider the environment before printing this e-mail*
From trojlita384otoj-at-gmail.com Mon Feb 19 10:46:40 2018 Return-Path: {trojlita384otoj-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [222.139.205.243] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id w1JGkcuX017428 for {microscopylistserverarchive6-at-microscopy.com} ; Mon, 19 Feb 2018 10:46:39 -0600 Received: from mail.webhostings4u.com [201.236.207.69] by mts.locks.grgtween.net with NNFMP; Mon, 19 Feb 2018 12:26:00 -0300 Received: from group21.345mail.com ([Mon, 19 Feb 2018 12:09:47 -0300]) by mmx09.tilkbans.com with QMQP; Mon, 19 Feb 2018 12:09:47 -0300 Received: from asx121.turbo-inline.com ([Mon, 19 Feb 2018 11:56:49 -0300]) by relay.2yahoo.com with ESMTP; Mon, 19 Feb 2018 11:56:49 -0300 Message-ID: {FB09E1E6.D2B09369-at-gmail.com}
Hi Everyone, I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.
We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 8, 40 -- From gilpin-at-purdue.edu Mon Feb 19 16:15:22 2018 8, 40 -- Received: from xppmailspam05.itap.purdue.edu (xppmailspam05.itap.purdue.edu [128.210.5.16]) 8, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1JMFMn2013820 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 19 Feb 2018 16:15:22 -0600 8, 40 -- X-Cloudmark-SP-Filtered: true 8, 40 -- X-Cloudmark-SP-Result: =?us-ascii?q?v=3D2=2E1_cv=3DU7Iydbfu_c=3D1_sm=3D2_tr?= 8, 40 -- =?us-ascii?q?=3D0_a=3DdSX5codZc0cA=3A10_a=3DIkcTkHD0fZMA=3A10?= 8, 40 -- =?us-ascii?q?_a=3Dx7bEGLp0ZPQA=3A10_a=3DxqWC=5FBr6kY4A=3A10_a=3DOp4juWPpsa0?= 8, 40 -- =?us-ascii?q?A=3A10_a=3D35Thz0BbAAAA=3A8?= 8, 40 -- =?us-ascii?q?_a=3D99ovruB2okNgMSt9=5FwEA=3A9_a=3DQEXdDO2ut3YA=3A10_a=3DOJSv?= 8, 40 -- =?us-ascii?q?EavBdRUA=3A10?= 8, 40 -- =?us-ascii?q?_a=3D72aGdUroxwQA=3A10?= 8, 40 -- X-IronPort-Anti-Spam-Filtered: true 8, 40 -- X-IronPort-AV: E=Sophos;i="5.46,536,1511845200"; 8, 40 -- d="scan'208";a="91973747" 8, 40 -- Received: from exchange.purdue.edu ([128.210.1.29]) 8, 40 -- by xppmailspam05.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 19 Feb 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl 8, 40 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 19 Feb 8, 40 -- 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 8, 40 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 8, 40 -- 15.00.1178.000; Mon, 19 Feb 2018 16:11:27 -0500 8, 40 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 8, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 40 -- Subject: evaporating Tin 8, 40 -- Thread-Topic: evaporating Tin 8, 40 -- Thread-Index: AdOpxe+Q9uh2u7unQXK2MI4XTzAWFA== 8, 40 -- Date: Mon, 19 Feb 2018 21:11:26 +0000 8, 40 -- Message-ID: {3a34e7f3e5834c789a3300e8572f0391-at-wppexc03.purdue.lcl} 8, 40 -- Accept-Language: en-US 8, 40 -- Content-Language: en-US 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 40 -- x-originating-ip: [10.163.23.200] 8, 40 -- Content-Type: text/plain; charset="utf-8" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w1JMFMn2013820 ==============================End of - Headers==============================
As the evaporated films get thicker, internal stress becomes significant. I'm not sure how bad tin films are but I've had issues with the films peeling when they got much over 0.1um thickness.
Good luck, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: gilpin-at-purdue.edu To: colijn.1-at-osu.edu Sent: 2/19/2018 5:17:35 PM
Considering that is more than 100 times the thickness you normally put down, that is an issue.
I tried using evaporation to put down a thick layer of antimony on glass before. First, when I calculated the mass of Sb to put down a micron at a certain distance, I found I had to fill my tungsten basket to the full and maybe even run a couple of times. Then I think I found the thermal stresses Henk described. The layer curled up off the glass.
Why do they want such a thickness?
Warren
-----Original Message----- X-from: gilpin-at-purdue.edu [mailto:gilpin-at-purdue.edu] Sent: Monday, February 19, 2018 4:17 PM To: Straszheim, Warren E [BIOTC]
Hi Everyone, I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.
We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!
Chris
Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
==============================Original Headers============================== 8, 40 -- From gilpin-at-purdue.edu Mon Feb 19 16:15:22 2018 8, 40 -- Received: from xppmailspam05.itap.purdue.edu (xppmailspam05.itap.purdue.edu [128.210.5.16]) 8, 40 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w1JMFMn2013820 8, 40 -- for {microscopy-at-microscopy.com} ; Mon, 19 Feb 2018 16:15:22 -0600 8, 40 -- X-Cloudmark-SP-Filtered: true 8, 40 -- X-Cloudmark-SP-Result: =?us-ascii?q?v=3D2=2E1_cv=3DU7Iydbfu_c=3D1_sm=3D2_tr?= 8, 40 -- =?us-ascii?q?=3D0_a=3DdSX5codZc0cA=3A10_a=3DIkcTkHD0fZMA=3A10?= 8, 40 -- =?us-ascii?q?_a=3Dx7bEGLp0ZPQA=3A10_a=3DxqWC=5FBr6kY4A=3A10_a=3DOp4juWPpsa0?= 8, 40 -- =?us-ascii?q?A=3A10_a=3D35Thz0BbAAAA=3A8?= 8, 40 -- =?us-ascii?q?_a=3D99ovruB2okNgMSt9=5FwEA=3A9_a=3DQEXdDO2ut3YA=3A10_a=3DOJSv?= 8, 40 -- =?us-ascii?q?EavBdRUA=3A10?= 8, 40 -- =?us-ascii?q?_a=3D72aGdUroxwQA=3A10?= 8, 40 -- X-IronPort-Anti-Spam-Filtered: true 8, 40 -- X-IronPort-AV: E=Sophos;i="5.46,536,1511845200"; 8, 40 -- d="scan'208";a="91973747" 8, 40 -- Received: from exchange.purdue.edu ([128.210.1.29]) 8, 40 -- by xppmailspam05.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 19 Feb 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc03.purdue.lcl 8, 40 -- (172.30.136.176) with Microsoft SMTP Server (TLS) id 15.0.1178.4; Mon, 19 Feb 8, 40 -- 2018 16:11:27 -0500 8, 40 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 8, 40 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 8, 40 -- 15.00.1178.000; Mon, 19 Feb 2018 16:11:27 -0500 8, 40 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 8, 40 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 8, 40 -- Subject: evaporating Tin 8, 40 -- Thread-Topic: evaporating Tin 8, 40 -- Thread-Index: AdOpxe+Q9uh2u7unQXK2MI4XTzAWFA== 8, 40 -- Date: Mon, 19 Feb 2018 21:11:26 +0000 8, 40 -- Message-ID: {3a34e7f3e5834c789a3300e8572f0391-at-wppexc03.purdue.lcl} 8, 40 -- Accept-Language: en-US 8, 40 -- Content-Language: en-US 8, 40 -- X-MS-Has-Attach: 8, 40 -- X-MS-TNEF-Correlator: 8, 40 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 40 -- x-originating-ip: [10.163.23.200] 8, 40 -- Content-Type: text/plain; charset="utf-8" 8, 40 -- MIME-Version: 1.0 8, 40 -- Content-Transfer-Encoding: 8bit 8, 40 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w1JMFMn2013820 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both chrisbrantner-at-gwu.edu as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: The George Washington University
Title-Subject: [Filtered] GWNIC EM position posted
Message: Greetings fellow microscopists,
I want to draw your attention to a position that I have open in my lab. If you are interested, please follow the links.
https://www.gwu.jobs/postings/49625
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X-from: Matt Russell {Matt.Russell-at-crick.ac.uk}
Hi Jan,
We have a CPD300 here that users have access to. One of our users is only trained on our bench top SEM and she uses it regularly, independently.
Top tips for things that might catch out students;
1. As per the manual, the fillers should be on top of the holders. If you have them the other way round the chamber doesn’t fill up correctly 2. Don’t overfill the chamber. Should be OK as long as the level is below the outlet hole at the top, otherwise you might have some liquid left at the end.
It’s easy to use with a simple user interface; it’s been easy to train people on it. We use it quite a lot and it gives nice results.
Cheers,
Matt
Matt Russell, PhD Senior Laboratory Research Scientist Electron Microscopy STP The Francis Crick Institute 1 Midland Road London NW1 1AT
} On 18 Feb 2018, at 17:36, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Factor, Jan {Jan.Factor-at-purchase.edu {mailto:Jan.Factor-at-purchase.edu} } } } } } Dear List: } } I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves } faculty and undergraduate student users for research projects, and trains undergraduates via } courses. After training, users carry out their own specimen preps. } } Two models I抳e come across are the Leica EMCPD300 and the Tousimis Autosamdri-931. } } I would appreciate any comments from folks who have experience with either of these, as well as } suggestions about other models I may have overlooked but should consider. } } Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu } {mailto:jan.factor-at-purchase.edu} } {mailto:jan.factor-at-purchase.edu} . } } --Many thanks, *Jan * } } Jan Robert Factor, Ph.D. } } Professor of Biology } } Purchase College, State University of New York } } Purchase, NY 10577 } } Office: 2016 Natural Science Bldg., 914-251-6659 } } Office Hours (Spring 2018): } } 牋牋燭ues., 11:00-12:00, and Thurs., 1:30-2:30 } } jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} {mailto:jan.factor-at-purchase.edu} } } /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally* } {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/ } *Please consider the environment before printing this e-mail* } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Sun Feb 18 } 10:53:43 2018 } 19, 53 -- Received: from mail-it0-f43.google.com {http://mail-it0-f43.google.com} } (mail-it0-f43.google.com {http://mail-it0-f43.google.com} [209.85.214.43]) } 19, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } w1IGrhv0021068 } 19, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; 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Cutting the slab is dangerous can may compromise the building. It is better to install active vibration isolation. I have a number of SEMs in Thousand Oaks, CA. One LaB6 system is on the 2nd floor. We use Herzan to reach vendor spec resolution with gold on carbon. Regards Gianni ________________________________________________________________________________________________________________________________ Gianpiero Torraca | Sr. Scientist | ATO Forensics | One Amgen Center Drive | Thousand Oaks, CA 91320 | Mailstop B30E-2B | 805.447.7445 “Mistakes are a fact of life. It is the response to the error that counts.” -Nikki Giovanni
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, February 16, 2018 12:46 PM To: Torraca, Gianni {gtorraca-at-amgen.com}
X-from: Darrell Miles {milesd-at-us.ibm.com}
One thing I don't think has been mentioned, is that the soil under many slab floors has settled, and the slab is "floating" above without being supported. I have heard of holes being drilled and a foam material being injected under the slab to support and dampen vibrations. This is done sometimes, in addition to other vibration isolation measures.
Regards, Darrell
microscopy.listserver-at-gmail.com wrote on 02/18/2018 11:56:14 AM:
} From: microscopy.listserver-at-gmail.com } To: milesd-at-us.ibm.com } Date: 02/18/2018 10:52 AM } Subject: [Microscopy] Fwd: viaWWW: Vibrations: cutting the slab? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/ } url? } u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=ugoYKYaSGvJRp_JnumnnfXAPUEYtdF3kIkvZE95DNGc&e= } On-Line Help https://urldefense.proofpoint.com/v2/url? } u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=FuLt6nOPYiSRyf8ejzJ0bIoZcEAz8a- } G0wqvuK3-bd0&e= } ---------------------------------------------------------------------------- } } X-from: Henk Colijn {colijn.1-at-osu.edu} } Reply-To: Henk Colijn {colijn.1-at-osu.edu} } To: microscopy.listserver-at-gmail.com } } Hi Bryan, } } Those are very low frequencies. They should be in a range that an } active compensation system should } be able to handle it. } } Another thing to consider is the resonant frequency of the slab } (even though it is damped by the } ground). The more mass in the slab, the lower its resonant } frequency. The install guides for our } microscopes show that the microscope is much more sensitive at very } low frequencies. The allowable } vibration at 10Hz is approx 10x lower than at 20Hz. Since too much } mass can push the slab resonance } down into the range where the microscope is more sensitive you may } want to estimate the resonant } frequency of your current slab and then consider whether to slice it } or not. It would also be } useful to measure the ground vibration away from the instrument to } get an idea of the driving } frequencies. } } Good luck! } Henk } } } } -------------------- } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212 } } colijn.1-at-osu.edu 614/643-3458 } cemas.osu.edu } } "Time is that quality of nature which keeps things from happening } all at once." (Ray Cummings - 1922) } Lately it doesn't seem to be working. } } ------ Original Message ------ } X-from: microscopy.listserver-at-gmail.com } To: colijn.1-at-osu.edu } Sent: 2/16/2018 4:45:19 AM } Subject: [Microscopy] viaWWW: Vibrations: cutting the slab? } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } } America } } To Subscribe/Unsubscribe -- } } https://urldefense.proofpoint.com/v2/url? } u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=ugoYKYaSGvJRp_JnumnnfXAPUEYtdF3kIkvZE95DNGc&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url? } u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=FuLt6nOPYiSRyf8ejzJ0bIoZcEAz8a- } G0wqvuK3-bd0&e= } } ---------------------------------------------------------------------------- } } } } X-from: btracy-at-eag.com } } } } This Question/Comment was submitted to the Microscopy Listserver } } using the WWW based Form at https://urldefense.proofpoint.com/v2/ } url? } u=http-3A__www.microscopy.com_MLFormMail.html&d=DwIBAg&c=jf_iaSHvJObTbx- } siA1ZOg&r=4k8urptLaVicBf1XZlZhMTu6vEfbbdyViST2saXOc5I&m=g9zPYPHRNYKozDFlGz7fVlDdc68JHnB1iQn_xboDrY0&s=0KEhg0YZd6xdGCuQ7_HIO_5vtgDgqDECxmHRxO0a1sM&e= } } --------------------------------------------------------------------------- } } Remember this posting is most likely not from a Subscriber, so when } } replying please copy both btracy-at-eag.com as well as the Microscopy } } Listserver } } --------------------------------------------------------------------------- } } } } Email: btracy-at-eag.com Name: Bryan Tracy } } } } Organization: EAG Laboratories } } } } Title-Subject: [Filtered] cutting the slab? } } } } Message: Hello Everyone, } } } } I know this is a subject on which reasonable people can disagree, but I } } wanted to ask what has been your experience with cutting the slab to } } reduce low frequency vibrations? } } } } The floor appears to be moving in the horizontal plane at 1.6Hz (4.4 } } reduction factor needed) and in the vertical direction at 5HZ. 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X-from: LNMA CMN Siglo XXI {lnma.cmn.sigloxxi-at-gmail.com}
Hi, Lisa. I am not an expert, but I would choose an analysis method knowing whether I will need to keep the seed, or it can be used entirely. Maybe I would try splitting it in parts (yep, it's millet, but still..) and then try different several fixation methods. I would also probably opt for hydration of the seed before fixation, but on an EtOH — to prevent germination[?] or fungal growth vapor bath. Interesting task!
Cheers, Vad
On Mon, Feb 19, 2018 at 9:03 AM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } To: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} }
Lisa,
Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve used it for corn and barley, but if you do have millet … oof.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
-----Original Message----- X-from: "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Reply-To: "microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Date: Sunday, 18February, 2018 at 12:29 To: Philip Oshel {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed
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X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} }
Thanks Phil. I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking this grain might have a more detrimental effect. Thanks for your advice
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Title-Subject: [Filtered] Two seats left! - BIO TEM workshop
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Hello, I am wondering if anybody can reccomend a standard/sample for determining microscope resolution in a SEM using the Fourier Ring Correlation (Autocorrelation) method. It is my understanding that suitable samples for TEM resolution estimates using FRC will not work in an SEM due to the differences in contrast mechanisms. Ideally, these would be samples which exhibit spatial frequencies from DC up to and beyond the resolution limit of the microscope.
Thank You, Nathaniel Rieders
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both rabara-at-tescan-usa.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: rabara-at-tescan-usa.com Name: Michal Rabara
Organization: TESCAN USA, Inc.
Title-Subject: [Filtered] Microscopy Position Posted
Message: Organization: Tescan USA, Inc.
Email response: rabara-at-tescan-usa.com Michal Rabara, CEO & President Tescan USA, Inc.
I would like to draw your attention to the Applications Manager position that we have open in North America for Tescan USA, Inc. If you are interested, please follow the link below:
https://www.linkedin.com/jobs/view/602245101/
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Email: jcaughey-at-su-group.com Name: Jim Caughey
Title-Subject: [Filtered] Zeiss Libra
Message: Does anyone know of a qualified ISO that can support this TEM - Zeiss Libra 120 Plus TEM?
Thanks for any help!
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Email: sorin.lazar-at-fei.com Name: Sorin Lazar
Organization: Thermo Fisher Scientific
Title-Subject: [Filtered] Applications Scientist TEM Semiconductors at Thermo Fisher Scientific, Eindhoven
Message: Dear TEM community,
I would like to draw your attention to an Applications Scientist TEM Semiconductors open position at Thermo Fisher Scientific in the Eindhoven Nanoport.
For those interested please follow the link below:
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Email: leonarddn-at-ornl.gov Name: Donovan Leonard
I am interested in learning what the potential cost of ownership of a Xe PFIB can be. In addition to the service contract, what costs associated with apertures and sources are there? I am assuming apertures have to be changed more frequently since beam currents can be on the order of microamps (depending on hours in use). What about the Xe gas? I think this is just a small lecture bottle of gas, but how often do you have to replenish that source?
Please respond off list to leonarddn-at-ornl.gov and thank you in advance. If you want to include any anecdotes about Xe PFIB ownership I am happy to learn about that too (e.g. nightmares or winning scenarios).
Donovan
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Email: sorin.lazar-at-fei.com Name: Sorin Lazar
Organization: Thermo Fisher Scientific
Title-Subject: [Filtered] job opening
Message:
"Dear TEM community,
I would like to draw your attention to an Applications Scientist TEM Materials Science open position at Thermo Fisher Scientific in the Eindhoven Nanoport.
For those interested please follow the link below: http://jobs.thermofisher.com/ShowJob/Id/156547/Applications-Scientist-TEM-Materials-Science/
Thanks and regards, Sorin Lazar "
With many thanks in advance, Sorin
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I just change the filament on a FEI Morgagni TEM. As I put the filament on the microscope and close the collum the PVP does not start.
I reset the system, open the column again, I have no idea of what to do next. Have anyone already had this problem? Any tips?
Thank you for your attention.
Best,
Pedro Leão
-- ---
Instituto de Microbiologia - UFRJ Laboratório de Biologia Celular e Magnetotaxia CCS - Centro de Ciências da Saúde - Bloco I Avenida Carlos Chagas Filho, 373 Cidade Universitária CEP. 21941-902 Rio de Janeiro - RJ - Brasil CV Lattes: http://lattes.cnpq.br/9781167318809464 LinkedIn:https://br.linkedin.com/in/pedro-leão-55992853 {https://br.linkedin.com/in/pedro-le%C3%A3o-55992853} Tel. +55 21 3938 6738 Cel. +55 21 98131 3360
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Email: millerme-at-ornl.gov Name: Madalynn Miller
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] ORNL Seeks Postdoc, Helium Ion Microscopy
Message: Postdoctoral Research Associate in Helium Ion Microscopy
Oak Ridge National Laboratory (ORNL) is looking for a Postdoctoral Research Associate to conduct Helium Ion Microscopy (HIM) research and Secondary Ion Mass Spectrometry (SIMS) of a broad range of inorganic and soft materials. Specifically, your research in this role will focus on imaging of soft materials. For this role, experience in mass spectrometry (MS) and helium ion microscopy (HIM) techniques, and/or other closely related areas is ideal. You will work with scientists and users at the CNMS in developing new HIM capabilities in chemical imaging using SIMS, multimodal data analysis, and integration with high performance computing environments. You will benefit from experience in the analysis and characterization of soft materials and inorganic materials.
For more details, including the qualifications for this position, visit: http://bit.ly/PostdocHIM
ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply.
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X-from: Gary Laevsky {glaevsky.lists-at-gmail.com}
Hi All!
We are very proud and excited to announce the formation of a new society, The North Atlantic Microscopy Society (NAMS). Geographically centered at Princeton, NJ, we envision our coverage to span southern New York, New Jersey and into Pennsylvania.
Edwin Hubble famously said, “Equipped with his five senses, man explores the universe around him and calls the adventure Science.” We believe that some of this exploratory instinct has been muted lately by our disciplinary silos. Individually, we have become exceptional in our specialties and do not take moments to appreciate the many discoveries happening across the entire spectrum of science.
Our mission is to bridge these silos through the lens of microscopy. We seek to achieve this mission by promoting microscopy education, stimulating networking among microscopists, and disseminating microscopy knowledge and skills to the public in the region.
Our first Symposium will be held at Princeton University on November 1, 2018.
Our first speakers will be;
Hari Shroff (Light) - Chief of NIBIB's Section on High Resolution Optical Imaging laboratory. Nieng Yan (Cryo-EM) - Shilrley M. Tilghman Professor of Molecular Biology at Princeton University.
This is exciting!!!
Gary Laevsky (Light Imaging) Paul Shao (EM Imaging) Tharan Srikumar (Mass Spec Imaging)
-- Best,
Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310
Greetings The deadline for paper submission has passed and meeting registration is now open. As you are making plans to attend the Baltimore, MD meetings (Aug. 5-9) please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.
The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events (paid by check at the end of the meetings). The jobs involve such things as providing support in the different symposia, staffing the volunteer office, newsletter distribution, and helping with vendor tutorial sign-up or in the outreach booth.
Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities they would like to help with. There is an additional bonus of $10 cash for each morning and/or afternoon session worked to assist with meals, along with a meeting t-shirt.
If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" on the registration form. Applicants for the bursaries must be a member of MSA or MAS and enrolled as a student at a recognized educational institution. Participants are responsible for their own registration fee and travel expenses.
For those of you who may not be students, but would still like to assist with the meetings, volunteers are also needed to fill the above-mentioned jobs. Although not paid the $10/hour as the students, volunteers do get a meeting t-shirt and the $10 cash for each morning/afternoon worked to help with meals.
If anyone has any questions about the bursary/volunteer program, or would like to participate, contact Amanda Lawrence: mailto:aml1819454-at-gmail.com (please do not reply to the email associated with this post). Bursary space is limited, so sign-up early.
See everyone in Baltimore,
Amanda Lawrence Student Bursary/Volunteer Coordinator Microscopy Society of America mailto:aml181954-at-gmail.com
==============================Original Headers============================== 10, 31 -- From ALawrence-at-i2at.msstate.edu Fri Mar 2 07:52:41 2018 10, 31 -- Received: from catalpa.its.msstate.edu (catalpa.its.msstate.edu [130.18.2.119]) 10, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w22DqfcQ003854 10, 31 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2018 07:52:41 -0600 10, 31 -- Received: from mail03.ad.msstate.edu (mail03.ad.msstate.edu [130.18.230.62]) 10, 31 -- by catalpa.its.msstate.edu (8.14.7/8.14.7) with ESMTP id w22CnLY9055835 10, 31 -- (version=TLSv1/SSLv3 cipher=ECDHE-RSA-AES256-SHA384 bits=256 verify=FAIL) 10, 31 -- for {microscopy-at-microscopy.com} ; Fri, 2 Mar 2018 06:49:21 -0600 10, 31 -- Received: from MAIL02.ad.msstate.edu (2620:0:1a30:1230::61) by 10, 31 -- mail03.ad.msstate.edu (2620:0:1a30:1230::62) with Microsoft SMTP Server (TLS) 10, 31 -- id 15.0.1347.2; Fri, 2 Mar 2018 06:49:21 -0600 10, 31 -- Received: from MAIL02.ad.msstate.edu ([fe80::7846:3039:9492:24b0]) by 10, 31 -- mail02.ad.msstate.edu ([fe80::7846:3039:9492:24b0%13]) with mapi id 10, 31 -- 15.00.1347.000; Fri, 2 Mar 2018 06:49:21 -0600 10, 31 -- From: "Lawrence, Amanda" {ALawrence-at-i2at.msstate.edu} 10, 31 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 31 -- Subject: Student help at the M&M 2018 meetings 10, 31 -- Thread-Topic: Student help at the M&M 2018 meetings 10, 31 -- Thread-Index: AdOyJN8aMcsLcBwdRPuv+ecrYrWhfA== 10, 31 -- Date: Fri, 2 Mar 2018 12:49:20 +0000 10, 31 -- Message-ID: {9bc64f025adc4dc2b9afd7f6f4ac6db9-at-mail02.ad.msstate.edu} 10, 31 -- Accept-Language: en-US 10, 31 -- Content-Language: en-US 10, 31 -- X-MS-Has-Attach: 10, 31 -- X-MS-TNEF-Correlator: 10, 31 -- x-ms-exchange-transport-fromentityheader: Hosted 10, 31 -- x-originating-ip: [130.18.230.93] 10, 31 -- Content-Type: text/plain; charset="us-ascii" 10, 31 -- MIME-Version: 1.0 10, 31 -- Content-Transfer-Encoding: 8bit 10, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w22DqfcQ003854 ==============================End of - Headers==============================
I am hoping someone could recommend somewhat-reliable software or (open source plug-in preferred) for extracting (approximated) 2D PSF from a pair of images of the same object, taken with closely matched contrast and same digital pixel count, but different beam current/diameter/profile. One of the images would be significantly sharper and serve as a reference, while the other "blurry" one is assumed to be a convolution of the reference image and PSF of the beam/instrument. The interest would be in extracting and examining PSF from the pair of images, convoluting extracted PSF with sharp image to estimate blurred one, and de-convoluting PSF from the blurred image to reconstruct sharp version. I am finding multiple plugins, but don't seem to come across something that would be convenient for all three operations.
Thank you very much beforehand,
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
I wanted to let you know that we’ve got two openings here at PNNL for an SEM technician and technologist to support imaging and sample preparation of nuclear materials. Candidates with a background in materials science and electron microscopy would be ideal.
Please feel free to forward the following links to anyone you think might be interested and don’t hesitate to contact me with any questions. Thank you.
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Message: Technical Associate Staff Member - Scanning Probe Microscopy
Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff Member to support scanning probe microscopy efforts at the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory (ORNL). In this role, you will be part of the Scanning Probe Microscopy Group, which is a leader in the development and application of scanning probe microscopies and spectroscopies to image and manipulate materials functionality in complex materials, ionic and electronic conductors, molecular assemblies, and nanoscale structures. A suite of 18 commercial and internally developed atomic force microscopes in ambient and controlled environments and scanning tunneling microscopes in ultrahigh vacuum systems are used for internal and user science, with emerging opportunities in enhanced data analytics and multimodal/chemical imaging.
For more details, including the qualifications for this position, visit: http://bit.ly/TP-SPM ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply.
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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave
Organization: DTU Nanotech
Title-Subject: [Filtered] PhD scholarship in In-situ TEM and Applications of Nanowire Vapor Phase Epitaxy Microsystems (VPE)
Message: We have a new PhD position open and I hope you will announce it on your website to help recruiting!
PhD scholarship in In situ TEM and Applications of Nanowire Vapor Phase Epitaxy Microsystems (VPE)
please apply online via http://www.nanotech.dtu.dk/about-ntch/ledige-stillinger/job?id=fd61bf71-1ffa-4e21-9a8c-d01c4c5f19a7
In this PhD project you will develop, fabricate and use microfabricated heater systems to study the processes of III-V nanowire growth with MOVPE in-situ TEM, thereby creating nanowire devices while imaging the process live!
Contact information: - Kristian Mlhave, DTU Nanotech, e-mail: kristian.molhave-at-nanotech.dtu.dk - Kimberly Dick Thelander, Solid State Physics, Lund University, e-mail: kimberly.dick_thelander-at-ftf.lth.se - Nika Akopian, DTU Photonics, e-mail: nikaak-at-fotonik.dtu.dk
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] thermal treatment of carbon-formvar film
Message: a researcher here wants to do some thermal treatments (up to 150C) of samples after they have been adhered to carbon-formvar coated silicon wafers (approx 13mm x 36mm) containing small holes, and inquired with me about the max temp these films can endure without failing. She has tested a blank film at her max temp and the film appeared to remain intact during heating, but fell apart when removed from oven and during cool down. We have searched for published info on thermal resistance of carbon-formvar but could not locate anything specific, so we are turning to the list for advice. The films are home-made and approx 25-30 nm thick for the formvar and approx 10nm carbon. thank you- Login Host: 149.169.81.41 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Message: Research Associate Electron Microscopy Technologist
Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a state-of-the-art Microscopy Shared Resource.
The individual should have extensive practical expertise in biological sample preparation for transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of confocal and widefield fluorescence microscopy would also be a plus.
The candidate will help users design innovative experiments and they will carry out sample preparation and imaging as well as assist in data interpretation.
Excellent verbal and written communication skills, ability to work with multiple users in a supporting role, and ability to work independently and proactively with limited supervision are essential. A Bachelors degree in biology or related discipline is required. One to three years of experience working in a Microscopy Shared Resource is preferred.
How to Apply
Interested individuals should apply for this position via the CSHL careers website at http://cshl.peopleadmin.com/postings/11688
Position Number 01779-R
Applicants should include a resume along with a description of their practical expertise and the names as well as email addresses of 3 references.
Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized internationally for its excellence in ground-breaking research programs in cancer, neuroscience, plant biology, genomics, and bioinformatics and broad educational mission.
For more information about CSHL, please visit us at www.cshl.edu
CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability or protected veteran status.
VEVRAA Federal Contractor
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Message: Dear listers. My first question is- Is osmium better purchased made up in vials or made from crystals in the lab. I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased from Polysciences.
Thank you in advance.
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Email: nyao-at-princeton.edu Name: Nan Yao
Organization: Princeton University Title-Subject: [Filtered] Princeton-Nature Conference: Frontiers in Electron Microscopy for the Physical and Life Sciences
Message: I would like to ask your help for including the news of a new Nature Conference in electron microscopy, in your future news update. Website link: http://www.nature.com/natureconferences/fempl2018/index.html
This new conference is entitled Princeton Nature Conference: Frontiers in Electron Microscopy for the Physical and Life Sciences, to be held at Princeton, New Jersey, July 11-13, 2018. Many internationally renowned scientists including 2017 Nobel Prize winner Dr. Joachim Frank will speak in this conference.
Thank you for your attention. Your help in posting this in the MSA community is greatly appreciated.
Best regards,
Nan Yao
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=} Osmium: I have the impression that 4% OsO4 (stored in sealed glass ampoules under nitrogen at 4 Celsius) staines less strong after storage for over 20 years (!); the crystalline form workswell after storage for more than 20 years (small tip for dissolving: I snap off the tip of the glass vial, add the water, seal tip with a little amount of parafilm/nescofilm and sonicate in in ultrasonic waterbath for ca 5 minutes(in fumehood))
=} Uranylacetate: I use UA bought in the early nineties, works perfectly...
greetings, Peter Heimann ( Cell Biology; Bielefeld University; Germany
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==============================Original Headers============================== 7, 26 -- From peter.heimann-at-uni-bielefeld.de Thu Mar 8 08:16:15 2018 7, 26 -- Received: from unibi-smtp-a.hrz.uni-bielefeld.de (unibi-smtp-a.hrz.uni-bielefeld.de [129.70.208.12]) 7, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w28EGEYj014190 7, 26 -- for {Microscopy-at-microscopy.com} ; Thu, 8 Mar 2018 08:16:14 -0600 7, 26 -- MIME-version: 1.0 7, 26 -- Content-transfer-encoding: 8BIT 7, 26 -- Content-type: text/plain; charset=iso-8859-15; format=flowed 7, 26 -- Received: from [192.168.178.21] ([217.247.95.107]) 7, 26 -- by unibi-smtp-a.hrz.uni-bielefeld.de 7, 26 -- (Oracle Communications Messaging Server 7.0.5.37.0 64bit (built Jan 25 2016)) 7, 26 -- with ESMTPPA id {0P590051KWPWB160-at-unibi-smtp-a.hrz.uni-bielefeld.de} for 7, 26 -- Microscopy-at-microscopy.com; Thu, 08 Mar 2018 14:13:14 +0100 (CET) 7, 26 -- X-Connecting-IP: [217.247.95.107] 7, 26 -- X-PMX-Version: 6.3.3.2656215, Antispam-Engine: 2.7.2.2107409, 7, 26 -- Antispam-Data: 2018.3.8.130615, AntiVirus-Engine: 5.46.1.0, 7, 26 -- AntiVirus-Data: 2018.3.8.5461002, pmx12 7, 26 -- X-EnvFrom: peter.heimann-at-uni-bielefeld.de 7, 26 -- Subject: Re: Osmium and UA question ( Peter Heimann ) 7, 26 -- To: Microscopy-at-microscopy.com 7, 26 -- References: {201803080449.w284nJC9011969-at-microscopy.com} 7, 26 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de} 7, 26 -- Message-id: {330a3059-2895-00c0-00bf-57d52b75f13f-at-uni-bielefeld.de} 7, 26 -- Date: Thu, 08 Mar 2018 14:13:01 +0100 7, 26 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:52.0) Gecko/20100101 7, 26 -- Thunderbird/52.6.0 7, 26 -- In-reply-to: {201803080449.w284nJC9011969-at-microscopy.com} ==============================End of - Headers==============================
In our hands, we always work with 1 g Osmium crystal (sealed ampoules). As we have a strong sample preparation load in the lab, the final solution (1-2%) is consumed in the next month aproximately, but I suppose that it is very stable.
In Uranile case, we have been using the powder from a bottle with more than 20 years without notice any degradation related to a new one.
In my opinion, the two substances are very stable over time.
Best regards
Juan Luis
El 08/03/2018 a las 6:08, microscopy.listserver-at-gmail.com escribió: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:Buchsmith-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } Buchsmith-at-gmail.com as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email:Buchsmith-at-gmail.com Name: JoAnn Buchanan } } Organization: Allen Institute } } Title-Subject: [Filtered] Osmium and UA question } } Message: Dear listers. } My first question is- Is osmium better purchased made up in vials or made from crystals in the lab. } I myself prefer the vials, I feel they are safer and the solution is protected from degradation. } } Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased } from Polysciences. } } Thank you in advance. } } Login Host: 63.237.233.8 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 11, 53 -- Frommicroscopy.listserver-at-gmail.com Wed Mar 7 22:48:42 2018 } 11, 53 -- Received: from mail-io0-f175.google.com (mail-io0-f175.google.com [209.85.223.175]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w284mfBi011663 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 7 Mar 2018 22:48:42 -0600 } 11, 53 -- Received: by mail-io0-f175.google.com with SMTP id 30so5513988iog.2 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 07 Mar 2018 19:45:41 -0800 (PST) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=f/Yu+VKdj9y3E27tzkveluSivokBGnkSx8d2uxvn0RQ=; } 11, 53 -- b=iX2qadp8mIy41M/gqeZJ2271E5oUA5yYpPWUoSaWMl1sQcPpMPCTRSXWqdvc0QupDP } 11, 53 -- n/bIyCMIk3tBZJQGcNJTk8u3jd1Zde5bd97JihT3F+5YHqJ/ScegQSgBO5uB4cmrvFH9 } 11, 53 -- K/cE/M9izr7JkgOuLcjbZhy/JAYnMRXZ7UI8x0C0vLWp+JpPJEXiPve5lzD8CJKggjz0 } 11, 53 -- tjU0TQHfZIiFoGd4+eu7lfFUvcdsDw85XyfXkNAlXgDfBB0nAARpLQ4g0gMLY9X651vu } 11, 53 -- LOM2GffkQW/rtU7ZUGSo9V0Y6MkrFQ0p9YpzQMKX71Afgm3RPSQeJVAHCg39vWRaLW+L } 11, 53 -- dJiw== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=f/Yu+VKdj9y3E27tzkveluSivokBGnkSx8d2uxvn0RQ=; } 11, 53 -- b=EAgVy0BoLXJ+ACYWgFOarLTOzRL7xtXPoPo/I1TSae5ZR4ka6PLF/YUzD6Y50BxRNJ } 11, 53 -- g01jSJo46q27ZpbNE/oY4u6Jdn+564gtUf3VvMPW1NN6W9XHIWFtpcrDe60WK4vxn8S9 } 11, 53 -- KH+2+aswmLZBJPfJl+lVh6nwv2OMVPXf+tqa2I7bPdXjR1w7LeWydqQbV/F/66zS2s+d } 11, 53 -- hJffVVoxpEp9IDvsXLXcBOm4W2QROQpJXsktTvQRZIr05ouXXtE+4HSXnn6je6XpfZDP } 11, 53 -- wniIJEy8fRGnB/LOBXHfkFlMETy6yoNqIDi9iP5OlhKj3WKUA7xVJfU4y6lBC3eKansk } 11, 53 -- YXDQ== } 11, 53 -- X-Gm-Message-State: APf1xPBwRWzuybdDmi49WClSLUFKMxR2S/k8wqXpD32ggf1AB+3h2lS4 } 11, 53 -- ebNfiLU5eUqbS9kvhNBfqRO6wo4h } 11, 53 -- X-Google-Smtp-Source: AG47ELvgfOZaRlUqNP9EllVdzSQbhZmL9D443zO9ij3nLRuiON0IOAY0OnD+6bCLSbcANCC4efArGQ== } 11, 53 -- X-Received: by 10.107.38.80 with SMTP id m77mr29464806iom.69.1520480740535; } 11, 53 -- Wed, 07 Mar 2018 19:45:40 -0800 (PST) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:14f1:a005:3aea:e3a8]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id 38sm13350272iom.48.2018.03.07.19.45.39 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 11, 53 -- Wed, 07 Mar 2018 19:45:39 -0800 (PST) } 11, 53 -- Subject: viaWWW: Osmium and UA question } 11, 53 -- References: {201803080201.w2821DPe001173-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {201803080201.w2821DPe001173-at-microscopy.com} } 11, 53 -- Message-ID: {dba7c4ff-11f6-8316-ce10-3e46ddb4b0d4-at-gmail.com} } 11, 53 -- Date: Wed, 7 Mar 2018 21:45:38 -0600 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 11, 53 -- Gecko/20100101 Thunderbird/52.6.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {201803080201.w2821DPe001173-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
-- /Juan Luis Ribas/ Servicio de Microscopía Centro de Investigación, Tecnología e Innovación Universidad de Sevilla Av. Reina Mercedes 4b 41012 Sevilla
Hi - want to share my experience with UA. About 10 yrs ago, I inherited a bottle of UA dated 1960s. I used it to do girds staining and negative staining. It did stain without any noticeable problems but then I got a new bottle UA of EMS from another PI, with which my staining looked fresher and less background. The bottom line is fresh US does give better staining. Your two-year-old UA should just be fine. Greg ---------------------------- Gang (Greg) Ning, Ph.D. Director, Microscopy Facility The Huck Institutes of the Life Sciences The Pennsylvania State University N-048 MSC University Park, PA 16802 USA Phone: 814-863-0994 Fax: 914-867-2587 Email: gxn7-at-psu.edu http://www.huck.psu.edu/facilities/microscopy-cytometry-up
----- Original Message ----- X-from: "microscopy listserver" {microscopy.listserver-at-gmail.com} To: "Gang Ning" {gxn7-at-psu.edu} Sent: Thursday, March 8, 2018 12:12:12 AM
X-from: Buchsmith-at-gmail.com
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Message: Dear listers. My first question is- Is osmium better purchased made up in vials or made from crystals in the lab. I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased from Polysciences.
Thank you in advance.
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Message: Technical Associate Staff Member - Scanning Probe Microscopy
Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff Member to support scanning probe microscopy efforts at the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory (ORNL). In this role, you will be part of the Scanning Probe Microscopy Group, which is a leader in the development and application of scanning probe microscopies and spectroscopies to image and manipulate materials functionality in complex materials, ionic and electronic conductors, molecular assemblies, and nanoscale structures. A suite of 18 commercial and internally developed atomic force microscopes in ambient and controlled environments and scanning tunneling microscopes in ultrahigh vacuum systems are used for internal and user science, with emerging opportunities in enhanced data analytics and multimodal/chemical imaging.
For more details, including the qualifications for this position, visit: http://bit.ly/TP-SPM ORNL is an equal opportunity employer. All qualified applicants, including individuals with disabilities and protected veterans, are encouraged to apply.
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Email: rvancamp-at-kettering.edu Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S
Message: We need to hasten our use of our ultramictrotome. I have identified a few of these still in use but, need more input to find facilities using this instrument on a daily basis that are also willing to train me in its operation. I have made progress on my own yet, have only been able to find one facility willing to provide training. I have also used the manual and made progress yet, I cannot state the manual for this instrument is well written. Finally, it is best for me to locate facilities within the Great Lakes region. I have searched for online training for this and have been unsuccessful.
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From purchase-at-upscmarine.com.sg Fri Mar 9 13:14:02 2018 Return-Path: {purchase-at-upscmarine.com.sg} Received: from 5t3.com (host42-30-237-212.serverdedicati.aruba.it [212.237.30.42] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w29JE1HJ030478; Fri, 9 Mar 2018 13:14:01 -0600 Message-Id: {201803091914.w29JE1HJ030478-at-microscopy.com} Received: from [103.207.38.155] (unknown [103.207.38.155]) by 5t3.com (Postfix) with ESMTPA id 7AC1E4DB3D; Thu, 8 Mar 2018 14:26:28 +0100 (CET) Content-Type: multipart/mixed; boundary="===============0965157591==" MIME-Version: 1.0
Once again I find myself turning to this most amazing of resources. I am casting a wide net for a micro CT operator and need your collective assistance. Many of you are closely associated with your local CT facilities or at least know the individuals providing the services. I am asking if you would be willing to pass this announcement along as I have not yet found a resource like this one in the scientific CT community.
The Smithsonian Institution National Museum of Natural History (NMNH) in Washington DC has an exciting opportunity to add a cone beam X-ray micro-computed tomography (mCT) operator in support of our new micro-CT Lab. The position is currently a 6 month contract with 2 options to extend providing for the scanning and modelling of a wide variety of natural science and museum specimens for the research staff of the NMNH.
The request for quote (RFQ) and the statement of work (SOW) are provided at the following link. Interested parties need to submit not later than COB April 13th, 2018 to be considered.
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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt
Organization: Texas A&M University
Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy
Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences at Texas A&M University seeks a highly motivated dedicated individual to work in a state-of-the-art microscopy shared resource.
The individual must have extensive practical expertise in biological sample preparation for transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of widefield fluorescence microscopy and confocal and would also be a plus. Excellent verbal and written communication skills and the ability to work with multiple users are essential. A doctoral degree in biology or related discipline is required. A minimum of three years of relevant experience in electron microscopy is required.
Interested individuals should apply for this position via the Texas A&M University website https://tamus.wd1.myworkdayjobs.com/TAMU_External
Position Number: R-003044
Applicants should include a resume along with a description of their practical expertise and contact information for 3 references. Texas A&M University is an Equal Opportunity / Affirmative Action / Veterans / Disability Employer.
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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt
Organization: Texas A&M University
Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy
Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences at Texas A&M University seeks a highly motivated dedicated individual to work in a state-of-the-art microscopy shared resource.
The individual must have extensive practical expertise in biological sample preparation for transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of widefield fluorescence microscopy and confocal and would also be a plus. Excellent verbal and written communication skills and the ability to work with multiple users are essential. A doctoral degree in biology or related discipline is required. A minimum of three years of relevant experience in electron microscopy is required.
Interested individuals should apply for this position via the Texas A&M University website https://tamus.wd1.myworkdayjobs.com/TAMU_External
Position Number: R-003044
Applicants should include a resume along with a description of their practical expertise and contact information for 3 references. Texas A&M University is an Equal Opportunity / Affirmative Action / Veterans / Disability Employer.
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X-from: snkeller-at-techie.com This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy both snkeller-at-techie.com as well as the Microscopy Listserver ---------------------------------------------------------------------------
Email: snkeller-at-techie.com Name: Sandra Keller
Organization: TA-Sicco
Title-Subject: [Filtered] TEM time?
Message: Hi All: I am looking to rent time on a TEM in good condition within a few hours of the DFW metroplex as soon as possible. The TEM that we normally use is down at the moment and I am looking for an instrument to use as a backup. I have many years of TEM experience and would require minimal training. Thanks in advance Login Host: 71.8.114.145 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 7, 53 -- From microscopy.listserver-at-gmail.com Mon Mar 12 14:31:56 2018 7, 53 -- Received: from mail-it0-f42.google.com (mail-it0-f42.google.com [209.85.214.42]) 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2CJVuxt001913 7, 53 -- for {microscopy-at-microscopy.com} ; Mon, 12 Mar 2018 14:31:56 -0500 7, 53 -- Received: by mail-it0-f42.google.com with SMTP id k79-v6so13046861ita.2 7, 53 -- for {microscopy-at-microscopy.com} ; Mon, 12 Mar 2018 12:29:43 -0700 (PDT) 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=gmail.com; s=20161025; 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; 7, 53 -- bh=AVLnXCzyU4/K9b/TKblCyGpdIJ8vCtlXVH0dTWWUF4U=; 7, 53 -- b=bBujQVanSt4cAwqTL5/aU283DhVzk50gRD/d4R0r/Riur5r74bfoVc4t5l+i/UizLf 7, 53 -- PbYWS3yKYX0HTc7/zzt0X6PyvIiXgqfxNXzHOTKwDZHAUW05oaw3EGfz11djz6WJnU2W 7, 53 -- BcGnyL5IxfrClisUzj7DvEYV5aJKC62Pu8n0Ih7Z5FqTfbElLtdbEKWcum9gQ28pq8YN 7, 53 -- +9Kf5Vysj0401IOQPwKlECweFg3fb04nV9Afj4UvD/cHPvncJtqn6LF1ABtEEJCJt4Vp 7, 53 -- 4Wv/IGcWc+KDoyp3iVWTUDniBIVNUBx7ZRfLUe/5Mfx5I7Hatr/PWIJ6C3iXnG23B23K 7, 53 -- X5ZA== 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=1e100.net; s=20161025; 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 7, 53 -- :user-agent:mime-version:in-reply-to:content-language 7, 53 -- :content-transfer-encoding; 7, 53 -- bh=AVLnXCzyU4/K9b/TKblCyGpdIJ8vCtlXVH0dTWWUF4U=; 7, 53 -- b=lim+o+3qJNj/1sCUF1YAu96fGl5yoBwuybT5r0wdB+twmV7iCDTLwe3Aa59xXWkm4F 7, 53 -- wnOYW0U/K+qokIw3GQlKz6IQQrg13JIyE2wtBPOAsgVPy02K63rUH8USiJv7KCBg5MC2 7, 53 -- HfVP/Oar7qCSZarFm6y61KlBCk68bfeAaA8bOMA/mOdUKsBD/QjWiw431gnaFOykv67e 7, 53 -- TK0/InQgkDa/EcKCMrDAxmvnFu9WXhcPVAbS+0uLzdlmIf05oqmlPNbOhjJ9ERywyNeV 7, 53 -- Zsq9dXU2pUNRRYPA5GRsIAj++vYkV/BVYbn0VAhVtIyjU8MB6UeukBsdiJoBBUWjSBtR 7, 53 -- 4FMg== 7, 53 -- X-Gm-Message-State: AElRT7FB95v8TCfGzVK2qdwYqs2EvKZxuSPO9Cb6q20DqamqcI4CeR6l 7, 53 -- lglWte15R147zxTRsiUM3RP2/3IM 7, 53 -- X-Google-Smtp-Source: AG47ELuhJvUihxdtQT+d6dRuvN7qROc95rFpWevEo9+FPqXqKMxLJ+fwgGva169phSsp0H1XOft1hg== 7, 53 -- X-Received: by 10.36.33.205 with SMTP id e196mr9711588ita.49.1520882982808; 7, 53 -- Mon, 12 Mar 2018 12:29:42 -0700 (PDT) 7, 53 -- Received: from eduroam062-140.wl.anl-external.org (eduroam062-140.wl.anl-external.org. [130.202.62.140]) 7, 53 -- by smtp.googlemail.com with ESMTPSA id y126sm3976258itc.29.2018.03.12.12.29.42 7, 53 -- for {microscopy-at-microscopy.com} 7, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 7, 53 -- Mon, 12 Mar 2018 12:29:42 -0700 (PDT) 7, 53 -- Subject: viaWWW: time on a TEM in the DFW area 7, 53 -- References: {201803121613.w2CGDBR9021533-at-microscopy.com} 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} 7, 53 -- X-Forwarded-Message-Id: {201803121613.w2CGDBR9021533-at-microscopy.com} 7, 53 -- Message-ID: {9d74fa38-21fb-825d-bf42-37565b6f45b8-at-gmail.com} 7, 53 -- Date: Mon, 12 Mar 2018 14:29:42 -0500 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 7, 53 -- Gecko/20100101 Thunderbird/52.6.0 7, 53 -- MIME-Version: 1.0 7, 53 -- In-Reply-To: {201803121613.w2CGDBR9021533-at-microscopy.com} 7, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 53 -- Content-Language: en-US 7, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] Job Opening at George Washington University
Message: The George Washington University Nanofabrication and Imaging Center (GWNIC) is seeking a Laboratory Associate or Technician dependent on experience and qualifications. Duties will include preparation and imaging of biological and materials samples for microscopy from our research labs. Additional duties will include general laboratory management. This is a support position in the GWNIC, for all of the departments of the University and outside users, where we provide high quality microscopy services. The minimum qualifications are as follows: Laboratory Associate: A BA/BS in a related discipline plus 2 years of relevant professional experience or, a Masters degree or higher in a relevant area of study. Degree must be conferred by the start date of the position. If you are interested in this position, apply at: http://www.gwu.jobs/postings/49493. Laboratory Technician: A high school diploma/GED plus 1.5 years of relevant professional experience, or, a Bachelors degree or higher in a relevant area of study. Degree must be conferred by the start date of the position. If you are interested, apply at: http://www.gwu.jobs/postings/49611.
The George Washington University is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.
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From davishal182newi-at-gmail.com Tue Mar 13 06:56:38 2018 Return-Path: {davishal182newi-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [182.126.148.246] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id w2DBubco009348 for {microscopylistserverarchive6-at-microscopy.com} ; Tue, 13 Mar 2018 06:56:38 -0500 Received: from unknown (HELO smtp.endend.nl) (Tue, 13 Mar 2018 03:46:00 -0800) by external.newsubdomain.com with SMTP; Tue, 13 Mar 2018 03:46:00 -0800 Received: from mxs.perenter.com [20.85.186.242] by m1.gns.snv.thisdomainl.com with NNFMP; Tue, 13 Mar 2018 03:37:30 -0800 Message-ID: {D029DE67.CD9B27A1-at-gmail.com}
X-from: tobi-at-stanford.edu
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Email: tobi-at-stanford.edu Name: Tobias Beetz
Organization: Stanford University
Title-Subject: [Filtered] Job at Stanford University - Transmission Electron Microscopy Scientist
Message: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM) scientist to lead the operations of the facilities FEI Titan Environmental TEM. The TEM scientist will provide training and support to researchers, maintain and optimize the microscope operation, work closely with equipment vendors, maintain and develop training procedures, develop and implement advanced techniques, assist and advice users on specimen preparation and data analysis, provide proof-of-concept service, and engage in research activities. The SNSF Titan ETEM is equipped with a spherical aberration corrector, EELS and EDS, a monochromator and high brightness gun, Lorentz, holography, and tomography capabilities, as well as a suite of in situ holders. S(he) will work to utilize this range of advanced techniques to the highest extent, making them known to the user community, matching appropriate techniques to the individual research projects. S(he) will interact with the broader research community and equipment vendors to be aware of advances in the field, and make recommendations and prepare proposals for future equipment purchases. S(he) will promote and collaborate in the publication and presentation of results and participate in conferences. Stanfords shared nanofacilities offer a comprehensive array of advanced nanofabrication and nanocharacterization tools. Over 1,000 researchers make use of the shared facilities each year in order to further their research programs. The goal of the shared facilities at Stanford University is to provide open, cost-effective access to state-of-the-art nanofabrication and nanocharacterization facilities for scientists and engineers from academia, small and large companies, and government laboratories. The FEI Titan TEM is organized under SNSF in the Electron & Ion Microscopy Suite which currently features a FEI Tecnai TEM as well as two FIB/SEMs and two SEMs. S(he) may supervise student trainers. The TEM scientist will report to the Faculty Director of SNSF.
For more information about SNSF, visit http://snsf.stanford.edu.
Application Deadline: Applications must be submitted by April 30, 2018.
X-from: Taylor, Jordan {J.W.Taylor-at-massey.ac.nz}
Hi
I would think that you could get a lesson on any microtome and be able to use this one - especially if it was one in the Leica family. I learnt on the Ultracut R and didn't need any training to step up to a EM UC 7. I wouldn't limit my learning oppurtunity to just this model of microtome if I were you - they are all essentially the same. Success will come with time and experience.
Maybe if you let the listserver know what problems you were having someone may be able to suggest a work through for you.
Best of luck!
Jordan Taylor Microscopy Technician Manawatu Microscopy and Imaging Centre Massey University Private Bag 11-222 Palmerston North, 4442 Ph +64 6 356 9099 extn 84719
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Saturday, 10 March 2018 6:27:08 a.m. *To:* Taylor, Jordan *Subject:* [Microscopy] viaWWW:Training Inquiry Re: Leica Reichert Ultracut S
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X-from: rvancamp-at-kettering.edu
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Email: rvancamp-at-kettering.edu Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S
Message: We need to hasten our use of our ultramictrotome. I have identified a few of these still in use but, need more input to find facilities using this instrument on a daily basis that are also willing to train me in its operation. I have made progress on my own yet, have only been able to find one facility willing to provide training. I have also used the manual and made progress yet, I cannot state the manual for this instrument is well written. Finally, it is best for me to locate facilities within the Great Lakes region. I have searched for online training for this and have been unsuccessful.
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I am planning to prepare lung tissue for TEM and Scanning Transmission X-ray Microscopy. An article by Jian Li (2009 Macromolecules 42) A new Approach to Studying Microcapsule Wall Growth Mechanisms stated they used an epoxy resin trimethylolpropane triglycidyl ether (TTE) and 4,4′-methylenebis(2-methylcyclohexylamine) (MBMCA) in a 1:1 weight ratio.
Is anyone familiar with this product? Is there a kit or would one need to purchase the reagents individually from Sigma etc?
Thank you for your suggestions Karen Kelley, UFL
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We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
I'm open to thoughts.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Oppsss.. Ohh damn! I meant to say our Amray is not set up for field emission.
Sorry about the confusion........ Frank
We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is ( correction add NOT) set up for field emission.
We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
I'm open to thoughts.
Stay safe.....
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called "Spitzenkathode".
Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through ion bombardment or burn the filament very easily.
I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a LaB6 cathode to bring all the advantages...
Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
It sounds like those are pointed tungsten filaments. In the days of prehistory (aka pre-FEG) in order to get a high brightness/coherence source people used pointed W cathodes. In order to get the tip of the needle hot enough to have reasonable emission, the hairpin ran very hot with the corresponding reduction in lifetime. If I remember, cathode lifetimes of ~10 hours were common. They would have been used for high-resolution SEM or high-coherence TEM imaging.
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: frank_karl-at-ardl.com To: colijn.1-at-osu.edu Sent: 3/14/2018 12:10:25 PM
I restored an Elmiskop Ia and I have a few boxes of original Spitzenkathode. They give much more coherence -- really impressive! But of course they don't last very long.
Does anyone know of another Elmiskop Ia still in operation?
-- Mike
On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Frank, } } a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called } "Spitzenkathode". } } Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more } coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through } ion bombardment or burn the filament very easily. } } I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a } LaB6 cathode to bring all the advantages... } } Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so... } } } Best wishes, } } Stefan } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.nanoflight.info } www.stefan-diller.com } www.electronmicroscopy.info } www.elektronenmikroskopie.info } www.zwillingsprojekt.de } www.assisi.de } Anfahrt: http://Mail.map24.com/Stefan.Diller } ----------------------------------------------------- } } Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission. } } } } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these. } } } } I'm open to thoughts. } } } } } } } } } } Stay safe........... } } } } Frank Karl } } Microscopist } } Akron Rubber Development Laboratory } } 2887 Gilchrist Road } } Akron, Ohio 44305 } } } } } } } } ==============================Original Headers============================== } } 10, 59 -- From frank_karl-at-ardl.com Wed Mar 14 10:16:24 2018 } } 10, 59 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0057.outbound.protection.outlook.com [104.47.40.57]) } } 10, 59 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2EFGOA7029396 } } 10, 59 -- for {microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 10:16:24 -0500 } } 10, 59 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 10, 59 -- d=ardlcorp.onmicrosoft.com; s=selector1-ardl-com; } } 10, 59 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; 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Applications are invited for Postdoctoral Research Scientist position in the area of thin film deposition and characterization by x-ray diffraction and electron microscopy at Columbia University. The films will be deposited by sputtering in an ultrahigh vacuum deposition chamber. The deposited films will be characterized by X-ray diffraction, scanning electron microscopy conventional and high-resolution transmission and scanning transmission electron microscopy as well as tomographic imaging, analytical electron microscopy and crystal orientation mapping. This is a full-time position and the work will be carried out in the thin film deposition lab, the shared materials characterization and electron microscopy facilities at Columbia University and at the ASRC of the City University of New York. The use of facilities at Brookhaven National Laboratories is also anticipated.
For more details please see http://apam.columbia.edu/katayun-barmak
-- Katayun Barmak Philips Electronics Professor Director, Materials Science and Engineering Program Department of Applied Physics and Applied Mathematics Seeley W. Mudd Building Columbia University 500 West 120th Street, Suite 200, MC 4701 New York, NY 10027-6623 Tel: (212) 854-8267 Fax: (212) 854-8257 Email: katayun.barmak-at-columbia.edu URL: http://apam.columbia.edu/katayun-barmak
==============================Original Headers============================== 4, 56 -- From kb2612-at-columbia.edu Wed Mar 14 17:39:43 2018 4, 56 -- Received: from outprodmail02.cc.columbia.edu (outprodmail02.cc.columbia.edu [128.59.72.51]) 4, 56 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2EMdhWh027488 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 17:39:43 -0500 4, 56 -- Received: from hazelnut (hazelnut.cc.columbia.edu [128.59.213.250]) 4, 56 -- by outprodmail02.cc.columbia.edu (8.14.4/8.14.4) with ESMTP id w2EMXX7f023684 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:36 -0400 4, 56 -- Received: from hazelnut (localhost.localdomain [127.0.0.1]) 4, 56 -- by hazelnut (Postfix) with ESMTP id 3C4A26D 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:38 -0400 (EDT) 4, 56 -- Received: from sendprodmail02.cc.columbia.edu (sendprodmail02.cc.columbia.edu [128.59.72.14]) 4, 56 -- by hazelnut (Postfix) with ESMTP id 1D8656D 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:38 -0400 (EDT) 4, 56 -- Received: from mail-qk0-f198.google.com (mail-qk0-f198.google.com [209.85.220.198]) 4, 56 -- by sendprodmail02.cc.columbia.edu (8.14.4/8.14.4) with ESMTP id w2EMbamD021330 4, 56 -- (version=TLSv1/SSLv3 cipher=AES128-GCM-SHA256 bits=128 verify=NOT) 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 18:37:36 -0400 4, 56 -- Received: by mail-qk0-f198.google.com with SMTP id t27so3154530qki.11 4, 56 -- for {Microscopy-at-microscopy.com} ; Wed, 14 Mar 2018 15:37:36 -0700 (PDT) 4, 56 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 4, 56 -- d=1e100.net; s=20161025; 4, 56 -- h=x-gm-message-state:to:from:subject:message-id:date:user-agent 4, 56 -- :mime-version:content-language:content-transfer-encoding; 4, 56 -- bh=1r6S670xqO2Q6F2DYh+zMe7DHwxYfWTKIfPeCSgWkSo=; 4, 56 -- b=BieFnMiD2tHCp3fk38Kx8su7AqBOiIiihovfJAv4lvi6jXhLTo5Ln3IzJWXgnekidI 4, 56 -- bdyVS5Tbh32xuteQYRfpnV9rvFrAQRyHixCxs/ffJrUHUpSp1AfC4x/LmOOQ30E6CC0n 4, 56 -- nEas56oHKstmGzhDI/agCWD5brToPo6PvQTh41l7RPGpxA+gd9nvs5qivXbQCicR4mK7 4, 56 -- 2pTc4wv9clBc+gO4naOMHhQjFhPmoCZPOJn6uV1hrt6J3TiotUD+cc5fizXGtMLts/Oq 4, 56 -- Agy4lsCbXjzaJ9VX+rUZQV8td9IhBPFIIdueTPoLyV2P5Bp/FTsUOSIwYWQHFMjlQtDl 4, 56 -- yfJA== 4, 56 -- X-Gm-Message-State: AElRT7EFLToFiQbxyq3t0z0xL9EsDv7hNQ4+UnW7VOr6dtqfScVotbZU 4, 56 -- V/0RbruIeseII/NnuvcyNM+UEPJscn/D2h6LArcUveZJysGU2lf0/pzfku6IFIfMur92joK6zqZ 4, 56 -- wer3yeLFSqwmeUJKQHZWO4Z5kkDOl 4, 56 -- X-Received: by 10.55.194.77 with SMTP id j13mr9459962qkm.213.1521067056002; 4, 56 -- Wed, 14 Mar 2018 15:37:36 -0700 (PDT) 4, 56 -- X-Google-Smtp-Source: AG47ELthv8+ZkqWZr2k20BHrsT1qQKpPcjw4aC1GgcFWF3MyivrdNc5OgHPLzmJJpMes7LXUqYbaGA== 4, 56 -- X-Received: by 10.55.194.77 with SMTP id j13mr9459944qkm.213.1521067055801; 4, 56 -- Wed, 14 Mar 2018 15:37:35 -0700 (PDT) 4, 56 -- Received: from [192.168.1.4] (ool-457c292f.dyn.optonline.net. [69.124.41.47]) 4, 56 -- by smtp.gmail.com with ESMTPSA id d198sm2345796qkg.91.2018.03.14.15.37.34 4, 56 -- for {Microscopy-at-microscopy.com} 4, 56 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 4, 56 -- Wed, 14 Mar 2018 15:37:35 -0700 (PDT) 4, 56 -- To: Microscopy-at-microscopy.com 4, 56 -- From: "Barmak K." {kb2612-at-columbia.edu} 4, 56 -- Subject: Post Doctoral Position at Columbia University 4, 56 -- Message-ID: {47b12b52-350c-955f-43d7-4b8faac0f25d-at-columbia.edu} 4, 56 -- Date: Wed, 14 Mar 2018 18:37:33 -0400 4, 56 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:52.0) Gecko/20100101 4, 56 -- Thunderbird/52.6.0 4, 56 -- MIME-Version: 1.0 4, 56 -- Content-Type: text/plain; charset=utf-8; format=flowed 4, 56 -- Content-Language: en-US 4, 56 -- Content-Transfer-Encoding: 7bit 4, 56 -- X-No-Spam-Score: Local 4, 56 -- X-Scanned-By: MIMEDefang 2.78 on 128.59.72.14 ==============================End of - Headers==============================
I did own an Elmiskop II and an Elmiskop 101 in the 80ies and 90ies.
Still I have a lot of special gaskets and o-rings, aperture holders and documents on these TEMs. Also tools etc...
If you or anybody else out there reviving an old SIEMENS machine needs parts, contact me ;-)
And: I still have some of the "Spitzenkathodes" and a lot of the normal ones.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Post-Doctoral Position in Thin Film Deposition and Structural Characterization by X-ray Diffraction and Electron Microscopy
The Department of Applied Physics and Applied Mathematics at The Fu Foundation School of Engineering and Applied Science of Columbia University in the City of New York invites applications for a Postdoctoral Research Scientist position in the area of thin film deposition and characterization by x-ray diffraction and electron microscopy.The films will be deposited by sputtering in an ultrahigh vacuum deposition chamber.The deposited films will be characterized by X-ray diffraction, scanning electron microscopy conventional and high-resolution transmission and scanning transmission electron microscopy as well as tomographic imaging, analytical electron microscopy and crystal orientation mapping.This is a full-time position and the work will be carried out in the thin film deposition lab, the shared materials characterization and electron microscopy facilities at Columbia University and at the ASRC of the City University of New York.The use of facilities at Brookhaven National Laboratories is also anticipated.//Candidates for postdoctoral research positions come to the University to continue their training, generally within three years of completion of Ph.D., for independent careers as scientists and scholars and must display strong research potential in their field of study. All candidates are expected to be able to work well in a team and to communicate effectively the results of their research or research activities both orally and in writing.
Minimum Qualifications:A PhD (or equivalent professional degree) in Materials Science and Engineering or a related field is required.
The candidate should have actual experience in operation and maintenance of sputtering systems, in x-ray diffraction studies of polycrystalline and epitaxial films, in scanning electron microscopy, and in conventional and high-resolution transmission, scanning transmission and analytical electron microscopy and crystal orientation mapping studies for physical sciences.Experience with tomographic imaging and image reconstruction is also highly desirable. The microscopy studies will be conducted on metallic films and lines.The candidate is required to prepare the necessary electron transparent samples for study using a variety of techniques such as conventional grinding, polishing and ion milling, chemical back etching, grid transfer techniques for 2D materials, as well as focused ion beam preparation.The candidate must be able to run experiments and analyze data independently.
Please send your CV, a cover letter and a list of three references with their contact information to Prof. Barmak at kb2612-at-columbia.edu {mailto:kb2612-at-columbia.edu} .The position is open immediately and will remain open until filled.
Columbia University is an Equal Opportunity/Affirmative Action Employer.
-- Katayun Barmak Philips Electronics Professor Director, Materials Science and Engineering Program Department of Applied Physics and Applied Mathematics Seeley W. Mudd Building Columbia University 500 West 120th Street, Suite 200, MC 4701 New York, NY 10027-6623 Tel: (212) 854-8267 Fax: (212) 854-8257 Email:katayun.barmak-at-columbia.edu URL:http://apam.columbia.edu/katayun-barmak
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Title-Subject: [Filtered] APRIL WORKSHOPS - SIGN UP NOW!
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We recently installed a Quorum cryo-SEM prep system to our Hitachi SU5000 SEM for imaging hydrogels. So far, the research groups I've been working with are not pleased with the results. I'm sure it's not the system but more so the lack of hydrogel structural knowledge seen by Cryo-SEM. Is there anyone in this group that can provide guidance? I can send images with our methodology.
Recently, I have been running a lot of negative staining of nanoparticles for the users of my core facility. A lot of the time, the particles are polymer spheres around 50nm in diameter. The issue is, if I spot just the negative stain (we use NanoVan) onto the grid with nothing else, I see a background of spherical, electron translucent "particles" with a dark border that are consistently between 20 and 40nm. The background is everywhere and at a fairly high density. I have images that I can share via personal communication with anyone who may be interested. With the background being so similar to many of the samples I work with, it is an unacceptable problem.
I believe I have narrowed down the source of said background. It is consistently there if I use brand new forceps, different and/or no pipettor, different stain altogether (2% uranyl acetate), or a new package of grids. However, if I glow discharge the grid immediately before use, the background disappears and I see a clean, smooth, stain as I should. This leads me to believe that there is either a particle left on the grid surface after manufacturing, or there are "patches" of non-uniform charge that are affecting the stain. The grids we are using are Formvar/ Silicon Monoxide coated 200 mesh copper grids from Ted Pella.
Has anyone else seen this type of background before, or does anyone have any ideas on what it might be? I plan to contact Ted Pella to see if they have any ideas as well.
Thank you, have a great weekend!
-Nick
Nicholas Conoan Electron Microscopy Specialist Wittson Hall 2014 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7292
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I am wondering if there is an open access database containing raw data of the secondary emission of electrons from various pure metal surfaces. The raw data would be something like the number of electrons N(E) versus the energy in the range from say 0-100eV.
I'm using Image J for some stereology work. It seems that I can't create a grid smaller than 1 square micrometer. Can anyone out there help?
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347 tbargar-at-unmc.edu
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Email: itbae-at-binghamton.edu Name: In-Tae Bae
Organization: State University of New York at Binghamton
Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools
Message: Dear Fellow microscopists, I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I contacted each manufacture for a new computer, they usually recommend a newer versions of software with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based computer anymore.) I have more that 10 tools whose computers are almost 10 years old and am looking for a more affordable way to replace these computers.
I wonder if anyone who deals with the same issue could enlighten me.
Thank you.
In-Tae Login Host: 128.226.88.12 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
==============================Original Headers============================== 12, 54 -- From microscopy.listserver-at-gmail.com Sun Mar 25 20:20:27 2018 12, 54 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) 12, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2Q1KRLR004343 12, 54 -- for {microscopy-at-microscopy.com} ; Sun, 25 Mar 2018 20:20:27 -0500 12, 54 -- Received: by mail-it0-f46.google.com with SMTP id z143-v6so10769221itc.0 12, 54 -- for {microscopy-at-microscopy.com} ; Sun, 25 Mar 2018 18:18:57 -0700 (PDT) 12, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 54 -- d=gmail.com; s=20161025; 12, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 12, 54 -- :in-reply-to:content-language:content-transfer-encoding; 12, 54 -- bh=sB5CnFoJ4x7DeQ90jGIt4vuPs8z2wFutMkqmsU1ISbk=; 12, 54 -- b=SRiTvkJEEP2ABG4HqRcqfs010iDEqRVB6ORu+9WlYc/ozc9BQRc0ayL1TYrqZZOq8O 12, 54 -- 6zlL++48am/BXV2BDIhHrpORt6KpYDk460oS06q/rZdO56pl9yhu/1qu1PppancbnIB/ 12, 54 -- WmYae5I7zV1DmCAGKOkXJP4IeT8Y8qi8Md87tgVIhiTAatJiSVXItM0Wqjt/eMjv8DPJ 12, 54 -- jsKgYO2k3z4hmQEQ9H3qdWPP4rcapIKXisBF2hLTDgv5JwjunePmGddrTJgjawfmauQ7 12, 54 -- FRkD8BWBT/oXPHqOFVdV0juXlcTCtCYsT4S9HeD+bMR36Y/nrGSgPMUJNDVFuelkOXeW 12, 54 -- wIvQ== 12, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 54 -- d=1e100.net; s=20161025; 12, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 12, 54 -- :user-agent:mime-version:in-reply-to:content-language 12, 54 -- :content-transfer-encoding; 12, 54 -- bh=sB5CnFoJ4x7DeQ90jGIt4vuPs8z2wFutMkqmsU1ISbk=; 12, 54 -- b=ceMJG26g3YO0s1EThVjNJYtEBrLfGf+7m38b60i5prIKU+wYTfUslgsapoyvrbyXWc 12, 54 -- aNPamaR2KFJQ9CgE5gsx1tPnnptyTkNa7F2Ut3ROew0/9sc/xXiYWqdpDzZWq/4dFjzf 12, 54 -- 0htyJOiLPxXidkXwR8dafIuhNtl01kRuULBJmjt1PQ/x+8JO4SHb45SLXHStqQYOkazh 12, 54 -- yHkuDcKIi4C6KTnTpnAJaszvye2DhIDxrCLo3QSdE30KcL0TsrU6FJxqrR+4Uwv6Pqgm 12, 54 -- PNaAw7+5EfXR9KLvJwMYJrzj2gPjEw3lFfHvo8MTNdykNOJ9T8Y61o27mf9wrZNtmy8v 12, 54 -- yGFg== 12, 54 -- X-Gm-Message-State: AElRT7Hln2fBYHPFqk3p5Dz041LfGM9my70+6f7hbgDCcoRmz0CwTMSp 12, 54 -- rRD8+M3nG9yPNPySrRAI7czVWW5g 12, 54 -- X-Google-Smtp-Source: AIpwx49eOatLwb0alxHRieUdd6T+ShjWs2KQT7uSTYBYupFqPHClO4kjpasxPplxZZxwqqjEedb0SA== 12, 54 -- X-Received: by 2002:a24:1c0e:: with SMTP id c14-v6mr5256580itc.106.1522027136811; 12, 54 -- Sun, 25 Mar 2018 18:18:56 -0700 (PDT) 12, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:44ec:f340:552e:fd6b]) 12, 54 -- by smtp.googlemail.com with ESMTPSA id e142-v6sm10151009ite.3.2018.03.25.18.18.55 12, 54 -- for {microscopy-at-microscopy.com} 12, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 12, 54 -- Sun, 25 Mar 2018 18:18:56 -0700 (PDT) 12, 54 -- Subject: viaWWW: How to deal with outdated computer for your TEM, SEM and 12, 54 -- other tools 12, 54 -- References: {201803212117.w2LLHnrR030469-at-microscopy.com} 12, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 12, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 12, 54 -- X-Forwarded-Message-Id: {201803212117.w2LLHnrR030469-at-microscopy.com} 12, 54 -- Message-ID: {c062cc4e-c96f-d0b1-2236-8dea5d27b709-at-gmail.com} 12, 54 -- Date: Sun, 25 Mar 2018 20:18:54 -0500 12, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 12, 54 -- Gecko/20100101 Thunderbird/52.6.0 12, 54 -- MIME-Version: 1.0 12, 54 -- In-Reply-To: {201803212117.w2LLHnrR030469-at-microscopy.com} 12, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 54 -- Content-Language: en-US 12, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Have you considered using an X-ray microscope? You can run scans at room temperature in air.
Aya
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, March 23, 2018 9:56 PM To: Aya Takase {Aya.Takase-at-rigaku.com}
X-from: Nessler, Randy A {randy-nessler-at-uiowa.edu}
We attempted this in the low vacuum mode of our Hitachi S-3400N, without luck. We resorted to using the cryostage. Best, Randy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, March 23, 2018 1:22 PM To: Nessler, Randy A
Dear In-Tae Bae,
There is a good reason why OEMs don't want to deal with Xp computers - replacing PCs is simpler and easier then maintaining older models, and being money-making organizations OEMs must recoup costs of developing and manufacturing upgrades with associated overhead; mentioned by you cost for computer/SW upgrade between US$10K to US$20K is actually quite reasonable, considering amount of design effort put into it.
That being said - you can reduce cost of dealing with forced obsolescence and refusal of service by trading money for maintenance effort.
First and foremost, make backup copies of hard-drives on your PCs, since software is the most "non-replaceable" part of the instrument and the HD failure is not "if," but "when" event. Then get online, scour websites of second-tier sellers, get necessary computer hardware (motherboards, processors, memory, peripheral cards, etc...), and build backup PCs for each and every instrument you have. If you have the expertise and time to do the work yourself, then virtually any PC can be rebuilt for much less then $1K in cost of parts. If you use one of the decent third-party support organizations, then cost of labor/travel would increase overall price, but is likely to still be lower then the price of new replacement PC from the OEM.
Alleged problem with Xp network security due to dropped support is easy to bypass by placing a cheap Win10 PC, or a Linux box, as a firewall between your Xp PC controlling the instrument and institutional network; images are passed through from instrument to network, but any other access to/from Xp PC is blocked.
Truly proprietary cards in PCs do pose some problem, although not all instruments have them. If there is such a card found, then depending on the level of technical expertise available you can either (a) try buying a card from OEM or third-party support organization, if available, or (b) monitor second-hand equipment market and snatch a PC from similar instrument when it comes up somewhere, or (c) rely on component-level repair to restore original card when and if it fails, (d) clone the proprietary card, many of them are quite simple, or (e) bite a bullet and upgrade PC by paying the OEM - which most of academic and institutional users do anyway.
Best Wishes, Valery
Valery Ray ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 - leave a message Mobie: +1-978-305-0479 - leave a message E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
To: vray-at-partbeamsystech.com Sent: Sunday, March 25, 2018 9:20 PM Subject: [Microscopy] viaWWW: How to deal with outdated computer for your TEM, SEM and
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Email: itbae-at-binghamton.edu Name: In-Tae Bae
Organization: State University of New York at Binghamton
Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools
Message: Dear Fellow microscopists, I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I contacted each manufacture for a new computer, they usually recommend a newer versions of software with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based computer anymore.) I have more that 10 tools whose computers are almost 10 years old and am looking for a more affordable way to replace these computers.
I wonder if anyone who deals with the same issue could enlighten me.
Thank you.
In-Tae Login Host: 128.226.88.12
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-------- Forwarded Message -------- Mon, 26 Mar 2018 12:22:46 +0000 X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
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Email: itbae-at-binghamton.edu Name: In-Tae Bae
Organization: State University of New York at Binghamton
Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools
Message: Dear Fellow microscopists, I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I contacted each manufacture for a new computer, they usually recommend a newer versions of software with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based computer anymore.) I have more that 10 tools whose computers are almost 10 years old and am looking for a more affordable way to replace these computers.
I wonder if anyone who deals with the same issue could enlighten me.
Thank you.
In-Tae Login Host: 128.226.88.12
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-------- Forwarded Message -------- Mon, 26 Mar 2018 12:22:28 +0000 X-from: Carol Heckman {heckman-at-bgsu.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
X-from: Henk Colijn {colijn.1-at-osu.edu}
Many times the issue with the custom PC cards is that they plug into an outdated bus. There are companies that still make motherboards and chassis components for the older interfaces. If the computer is failing but the cards are still OK, you can get a new PC system and "just" plugin the cards. It may require some fiddling to get things to work but at least you can get a few more years from your system.
I just did a web search and found "Nixsys.com" that sells ISA bus PCs. "Cyberresearch.com" also has passive backplanes with ISA slots. There is no motherboard per se; you add a processor card to the system. (I have no interest in either company)
Cheers, Henk
--------------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922) Lately it doesn't seem to be working.
------ Original Message ------ X-from: microscopy.listserver-at-gmail.com To: colijn.1-at-osu.edu Sent: 3/26/2018 9:27:46 AM
-------- Forwarded Message -------- X-from: James M. Ehrman {jehrman-at-mta.ca}
On a related note, our new (2016) Hitachi SEM and Oxford EDS are banned from accepting Windows 7 updates (by Hitachi) - updates beyond the Windows installation they provided will apparently break the software. I have them connected together via a router, with both being denied Internet access by giving them a bogus Default Gateway in the Local Area Connection setup. Another computer (with Internet access granted) is also connected to the router, and we get data from the two instruments out to the real world through drive shares. Not elegant, but it works. And I have to say that having two computers that never nag about updates or suddenly become broken when M$ screws up some driver file that the instrument depends on is quite refreshing!
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
} } } 26.03.2018 at 15:26: } We just linked the old XP machines to another computer running Windows 7 } which is secure. I don't } know exactly how to do this ‑ check with your IT department. They can } probably fix you up.
the tricky thing is that even Windows 7 will not be supported (by MS) any more in 1 or 2 years time ... we will not have fun with "old" computer systems. regards - Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 Next microscopy conferences: - 19th International Microscopy Congress, Sydney; 9-14 Sept 2018 http://imc19.com/ - Microscopy Conference MC2019: 1.-5. Sept 2019 in Berlin - next Microbiol. conferences: VAAM - Annual Conf.: 15-18 April 2018 Wolfsburg
Hello Everyone, The story goes, there once was a fella who ran EDS on a rubber sample and found less 1% (semi quant mode). But another lab under the corporate umbrella used XRF as well as ICP and found 5%.
I got my theories and hand waving, but I can't really help my co-worker out. I'm open to theories. What does the collective wisdom of the microscopy community say?
Thanks........
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
You didn't say, but I am guessing you meant they were analyzing for sulfur. I will presume so for this discussion.
If you were measuring S in rubber, that would mean a lot of C in the matrix. I would REALLY doubt the C number from semi-quant mode. C may have been dreadfully inflated and thus would have diluted the S content. Some systems may be better than others but I am always surprised when the C comes out even halfway close to right in semi-quant mode.
I would rather try to measure C(+H) by difference. That would mean going somewhat beyond semi-quant mode. You would have to turn off normalization and calibrate your beam intensity to that used to collect the standards. Specify C as the element to be determined by difference. I think the resulting S number would be much more reasonable. (At least it was when I did my MS work nearly 40 years ago. They accepted it for my thesis work.) I know how to do that on Oxford equipment today, and I had done that on Noran equipment for my masters. I don't know how the others would set that up.
Warren Straszheim
I only got 22 out-of-office messages to my last post. What will I find this time?
-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: Tuesday, March 27, 2018 12:27 PM To: Straszheim, Warren E [BIOTC]
Hello Everyone, The story goes, there once was a fella who ran EDS on a rubber sample and found less 1% (semi quant mode). But another lab under the corporate umbrella used XRF as well as ICP and found 5%.
I got my theories and hand waving, but I can't really help my co-worker out. I'm open to theories. What does the collective wisdom of the microscopy community say?
Thanks........
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
My advice to you is to trust the XRF and ICP results over the EDS.
It is always a big problem to perform quantification on a non-conductive material such as rubber in SEM. Under the typical SEM conditions, the electron beam will induce localized charging on the scanned region of interest. Charging is a big problem and causes unpredictable data in the EDS spectrum. Also to get an accurate quantification in EDS, you have to make sure you acquire a statistically significant amount of X-ray counts as recommended by the EDS manufacturer. Finally, EDS is also a surface sensitive technique and so you have to make sure that the region of interest is not coated with some additional layer foreign material (such as powder or grease)
Most layman users also stick to the easy-way-out Normalized method of quantification, which is another problem if you have source of uncertainty in other elements. As pointed out by Warren, one element that tends to build up over time is Carbon. Hence, assuming Normalized quantification was used, the data will appear to have higher C%, and resulting in lower S% for example.
In conclusion, if the EDS data was not acquired by a well-trained SEM-EDS user, the result should not be trusted with high degree of confidence. You should advise your co-worker to contact the local support for the EDS provider to confirm whether data acquisition methodology was applied correctly. I am also open to discussion on the parameters you used for acquiring the EDS data on the rubber samples.
Eugene eugene.choo-at-oxinst.com
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Wed, 28 Mar, 2018 7:23 AM To: CHOO Eugene
You didn't say, but I am guessing you meant they were analyzing for sulfur. I will presume so for this discussion.
If you were measuring S in rubber, that would mean a lot of C in the matrix. I would REALLY doubt the C number from semi-quant mode. C may have been dreadfully inflated and thus would have diluted the S content. Some systems may be better than others but I am always surprised when the C comes out even halfway close to right in semi-quant mode.
I would rather try to measure C(+H) by difference. That would mean going somewhat beyond semi-quant mode. You would have to turn off normalization and calibrate your beam intensity to that used to collect the standards. Specify C as the element to be determined by difference. I think the resulting S number would be much more reasonable. (At least it was when I did my MS work nearly 40 years ago. They accepted it for my thesis work.) I know how to do that on Oxford equipment today, and I had done that on Noran equipment for my masters. I don't know how the others would set that up.
Warren Straszheim
I only got 22 out-of-office messages to my last post. What will I find this time?
-----Original Message----- X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com] Sent: Tuesday, March 27, 2018 12:27 PM To: Straszheim, Warren E [BIOTC]
Hello Everyone,
The story goes, there once was a fella who ran EDS on a rubber sample and found less 1% (semi quant mode). But another lab under the corporate umbrella used XRF as well as ICP and found 5%.
I got my theories and hand waving, but I can't really help my co-worker out. I'm open to theories. What does the collective wisdom of the microscopy community say?
Thanks........
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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Email: kathleen.g.roberts-at-rutgers.edu Name: KATHLEEN ROBERTS
Organization: RUTGERS UNIVERSITY
Title-Subject: [Filtered] Repairing a Zeiss EM 109
Message: To all,
We have a Zeiss EM 109 that is acting up. Is there a company (or someone) that is reasonably local to Rutgers (Piscataway) who can come look at it? Our previous repair person retired.
Thank you so much for all your help, Kathleen
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Email: dartjulia-at-bfusa.com Name: Julia
Organization: Bridgestone
Title-Subject: [Filtered] AFM Scientist Job Opening Message: Dear all,
Bridgestone Americas Center for Research and Technology is currently seeking an experienced Research Scientist with expertise in Microscopy to join an interdisciplinary team of scientists and engineers working to solve problems at the leading edge of tire technology.
Ph.D. in Material Science, Chemistry, Physics, or a related technical discipline in chemistry or other science related disciplines. Ability to independently lead research combining advanced analytical method development with material science, polymer science, and tire technology needs. Extensive hands on experience in AFM, SEM, TEM, quantitative image analysis, and statistical analysis is desired. Location: Akron, OH
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Email: m.aindow-at-uconn.edu Name: Mark Aindow
Organization: University of Connecticut
Title-Subject: [Filtered] Research Scientist /Senior Research Scientist - Electron Microscopy
Message: Tech Park, University of Connecticut
Position ID: UConn-TechPark-2018299 [#10893, 2018299] Position Title: Research Scientist /Senior Research Scientist - Electron Microscopy Position Type: Non tenure-track faculty Position Location: Storrs, Connecticut 06269, United States [map] Subject Area: Innovation Management Appl Deadline: none (posted 2018/02/19) Position Description: https://academicjobsonline.org/ajo/jobs/10893/apply Reporting directly to the Executive Director of the Innovation Partnership Building (IPB) at the UConn Tech Park, the Research Scientist will be engaged in research, securing extramural funding, planning experiments, evaluating, interpreting and publishing results. This position would have a May 2018 start date, and will be renewed annually contingent upon research productivity and successful generation of extramural sponsored projects, including, industry agreements and grant funding.
The successful applicant will be expected to maintain an independent and/or collaborative program of research supported by extramural funding, including writing proposals, running research projects and publishing high quality reports and other publications
The incumbent will also: operate and maintain equipment related to Electron Microscopy including SEM, STEM, TEM, and high resolution TEM ; develop new procedures and modify or custom-design complex lab equipment and resolve design and malfunction problems of equipment; perform highly specializes tasks requiring precision and accuracy involving repeatable results; resolve difficult problems with sophisticated and sensitive lab instruments; Instructs others in the proper and safe use of a variety of laboratory equipment, material and chemical handling processes, bottled gases and associated distribution systems, laboratory materials and chemicals, ventilation systems, high temperature furnaces, presses etc.; and may train and or oversee the work of other staff including technical support for material characterization instruments at the Innovation Partnership Building
MINIMUM QUALIFICATIONS
PhD in Material Science, Chemistry, Physics or other relevant fields. Five plus years of postdoctoral or industry experience in a relevant field. Experience with aberration corrected Electron Microscopy. Professional interpersonal skills and the demonstrated ability to work effectively as a team player with a wide variety of individuals. Strong written and communications skills. Demonstrated ability to handle multiple tasks and activities while simultaneously ensuring problems are resolved efficiently and effectively. PREFERRED QUALIFICATIONS
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Two years ago, Tina Carvalho put on the list server “How do I make my smart phone into a microscope?” She got lots of replies. I have been collecting quite a lot of them. My favorites will be at the Outreach Booth at M&M in Baltimore in August. Some work better than others and a lot of the results depends on how good your smart phone camera is. I still use the Echo Wooden Microscope with my smart phone when I take a plankton tow.
I wondered how difficult it would be to make a Lego version. I borrowed some Lego from my son and made one. However, the permutations of what is possible with Lego seemed extensive, so I am putting forward “The Great Lego Microscope Competition”.
Place: M&M Meeting, Baltimore 2018, Outreach Booth Lenses: the designer has to take care of. Simple lenses work fine for this project. Technical Lego can be used. Prizes: yes
I will have the version I made, and I hope I have given you enough time to put something together to bring to the meeting.
I will bring some Lego to one of the stations in “Microscopic Explorations” (used to be Family Affair), on the Wednesday afternoon, to make a Lego Microscope. While I am on the subject of Microscopic Explorations, which is for delegates, their families and friends, this year we should have “how to put together a Foldscope, and activities to use with the Foldscope including a couple of solve the mysteries.” All attendees should take away their own put-together Foldscope and be comfortable using it.
Best wishes Elaine
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada
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Email: duraine-at-bcm.edu Name: Lita Duraine
Organization: Howard Hughes Medical Institute
Title-Subject: [Filtered] RE: [Microscopy] The Great Lego Microscope Competition
Message: A LEGO microscope would be cool.
In 2017 there was a push for the LEGO factory to make a LEGO Transmission Electron and Scanning Electron Microscopes (not working of course). People could vote for it. Here is the web page: https://ideas.lego.com/projects/102b2832-4574-4870-95a0-8698211d3fdf
Not sure what happened to the idea, haven't seen any in the stores. But it would be neat to have in the lab. Lita
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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Organization: Ravi Thakkar
Title-Subject: [Filtered] Liposome with An Nanopaticle attached.
Message: Hi all,
I have user having liposome sample attached with Au nanoparticle by additional functional group and its control sample (liposome and Nanoparticle) without functional group attached. Both samples look great under TEM.
But how to identify the Au Nanoparticles are attached to Liposomes by functional group or randomly seated on liposome. If anyone here can give some idea.
Thanks.
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The problem of out of date EM computers has been going on for many years and when training service technicians I encouraged then to educate the customer on this. At the time of an EM installation the laboratory will usually be flooded with PC, so we suggest that when replacing that equipment the laboratory keeps a couple of the redundant units complete with keyboard and mouse as back up spares. How often when training operators did we have a failure, fan, transformers, keyboard etc, when with redundant PC available we were up and running again in no time. Being prepared is a good idea. Regards
Steve
Steve Chapman FRMS Protrain for Consultancy and Training in Electron Microscopy +44 (0)7711 606967 web www.emcourses.com
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: 26 March 2018 02:22 To: protrain-at-emcourses.com
X-from: itbae-at-binghamton.edu
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Email: itbae-at-binghamton.edu Name: In-Tae Bae
Organization: State University of New York at Binghamton
Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools
Message: Dear Fellow microscopists, I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I contacted each manufacture for a new computer, they usually recommend a newer versions of software with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based computer anymore.) I have more that 10 tools whose computers are almost 10 years old and am looking for a more affordable way to replace these computers.
I wonder if anyone who deals with the same issue could enlighten me.
Thank you.
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Steve Chapman's recent note matched our lab practice of keeping a supply of suitable replacement PCs.
I did want to add a couple of suggestions:
1. Use a suitable hard disk backup program (we used an old copy of Norton Ghost) to make a backup hard drive for your instrument when it is fully configured. This saved our bacon many times. You will be glad you did because you can spend days rebuilding an instrument PC from scratch. Unless you are a real packrat, you likey won't have all the service pack installers to get a vanilla install to where you need to be.
2. Secure network file shares that are properly backed up and archived are worth the effort. Create one. Store everything you need for the instrument in a folder tree for that instrument. I created a "site book" document for the instrument in the root folder. Add all your notes for maintenance and sys admin to that document. A wise statistician I know quipped, "Your closest collaborator is you six months from now. And you don't respond to email." Let me add a couple of examples: 1) what is the admin password to the UPS unit so I can reset the battery lifetime after I replaced the batteries to turn of the alarm? 2) What is the part number for the correct filter for the new chiller? 3) What was that command sequence the service engineer showed me 9 months ago that let me get to the vacuum diagostic panel to see why my microscope won't pump down properly? You get the idea...
While you are at it save PDF copies of all the manuals and installers for any software you need on that network share in the appropriate folder. Save all the electronic copies of the service reports there. This folder will be your one-stop for everything you need for the instrument. No more questions of "Where did I file that?" You can set different access levels for different people who might need to see the info. Trust me, future you will be very happy that present you did this.
Best regards, John Minter Retired microscopist from Kodak Analytical Sciences
John; Good advice, but even better than Norton Ghost is cloning. I have had service engineers ‘ghost’ hard drives many times, and EVERY time the ghost was needed to restore a system, it failed. For less than the cost of Ghost, you can buy a new hard drive, clone it, and keep the clone around for when something happens to your system disk. I have never seen a clone fail, plus you can test it right away to see if it is good. One note: Acronis recommends never leaving the cloned disk and the original disk connected to the bus; attempting to boot the computer with both drives connected can corrupt the boot sector of the new clone.
A. John Mardinly, Ph.D., P.E. Also Retired
} } } } -------- Forwarded Message -------- } } X-from: John Minter {jrminter-at-gmail.com} } } } } Steve Chapman's recent note matched our lab practice of keeping a supply of suitable replacement PCs. } } I did want to add a couple of suggestions: } } 1. Use a suitable hard disk backup program (we used an old copy of Norton Ghost) to make a backup } hard drive for your instrument when it is fully configured. This saved our bacon many times. You } will be glad you did because you can spend days rebuilding an instrument PC from scratch. Unless you } are a real packrat, you likey won't have all the service pack installers to get a vanilla install to } where you need to be. } } 2. Secure network file shares that are properly backed up and archived are worth the effort. Create } one. Store everything you need for the instrument in a folder tree for that instrument. I created a } "site book" document for the instrument in the root folder. Add all your notes for maintenance and } sys admin to that document. A wise statistician I know quipped, "Your closest collaborator is you } six months from now. And you don't respond to email." Let me add a couple of examples: 1) what is } the admin password to the UPS unit so I can reset the battery lifetime after I replaced the } batteries to turn of the alarm? 2) What is the part number for the correct filter for the new } chiller? 3) What was that command sequence the service engineer showed me 9 months ago that let me } get to the vacuum diagostic panel to see why my microscope won't pump down properly? You get the idea... } } While you are at it save PDF copies of all the manuals and installers for any software you need on } that network share in the appropriate folder. Save all the electronic copies of the service reports } there. This folder will be your one-stop for everything you need for the instrument. No more } questions of "Where did I file that?" You can set different access levels for different people who } might need to see the info. Trust me, future you will be very happy that present you did this. } } Best regards, } John Minter } Retired microscopist from Kodak Analytical Sciences } } ==============================Original Headers============================== } 13, 53 -- From microscopy.listserver-at-gmail.com Sat Mar 31 13:49:15 2018 } 13, 53 -- Received: from mail-it0-f43.google.com (mail-it0-f43.google.com [209.85.214.43]) } 13, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w2VInFMT031369 } 13, 53 -- for {microscopy-at-microscopy.com} ; Sat, 31 Mar 2018 13:49:15 -0500 } 13, 53 -- Received: by mail-it0-f43.google.com with SMTP id r19-v6so14930880itc.0 } 13, 53 -- for {microscopy-at-microscopy.com} ; Sat, 31 Mar 2018 11:48:04 -0700 (PDT) } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=gmail.com; s=20161025; } 13, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 13, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 13, 53 -- bh=cJ3p92SfEh4wY55Mt+dMCwWZxFGJGNd410Gm+tKJENI=; } 13, 53 -- b=PwGK5WSVBqCTlLl1nKQCkfOSSwUoK7Meya8NwGqpR3DsOTTh2LKYrydANLN3mxlqIR } 13, 53 -- BP/HE4b/bP5IyMziz60JRDVsMdBv/wNhm2JYk65J57yOkjI2V8FD405sTtOGSM84U5Uf } 13, 53 -- gkCoJNVD5kt9fxUv2194Bl5yhIuMugGZkHNjVEVLyFkQqEPxtyC51aIqVCQ5sHic72F2 } 13, 53 -- h9EJuLOCKpz4Fy7chhSlSDos8SvNasmjH86ZLzyQmJ3bKXmyTjYpfte0xh6KncXnnySY } 13, 53 -- FKQNMskoioOdX1aUqvzBtiwE7Bc6t6kMbAjGLLYgp7LRfLNyHsnSZ+jGliocJIAfl/cb } 13, 53 -- jUAQ== } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=1e100.net; s=20161025; } 13, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 13, 53 -- :user-agent:mime-version:in-reply-to:content-language } 13, 53 -- :content-transfer-encoding; } 13, 53 -- bh=cJ3p92SfEh4wY55Mt+dMCwWZxFGJGNd410Gm+tKJENI=; } 13, 53 -- b=AdjMTPMex1rcm3uXdnsQvzfn4/+5CYgg1L37SKe8L1jKpNDjskkmcg0/uUfLUZnOrm } 13, 53 -- m5Vef5LprX0K1xPo1jdDYzBr2belsEKTWtR4KMuspslVwROwyOWBs+EvovbQMfTgi3Ca } 13, 53 -- d+Mj6x55HJZiG+mPC8aTAPT9bI0J5E9JSi6f9/rxw+wRPsTbjc431zRQYaiheHhvD88A } 13, 53 -- NQbQFjzfqjSrLCp3kmu7fS51urZcdL2KWbqGNv4xpVSH6+WnZncIsr9OQjs7btm8QFrz } 13, 53 -- R44rJyarGD5e7nioFXNRRLrB2AQXSLfF1EAkTaRgd6KQY+grfxrKL9N82yu2ZHMxPKqX } 13, 53 -- WJnA== } 13, 53 -- X-Gm-Message-State: ALQs6tAbOH12ceuKFNOpDwpk9Byus0Ka8blhekprLsT2jcc4ypxEEWlx } 13, 53 -- CHY8Ga8SKDO1lxZP+bLLpkrmepCO } 13, 53 -- X-Google-Smtp-Source: AIpwx4/IGnZemLTajs+rjPI0v8nB4ChWMY/VKmExG6MQPGGYsKXbDvKGawc0RCvtSS359Xt01FuhgA== } 13, 53 -- X-Received: by 2002:a24:1889:: with SMTP id 131-v6mr5203888itr.104.1522522083667; } 13, 53 -- Sat, 31 Mar 2018 11:48:03 -0700 (PDT) } 13, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:f953:7ca7:fc18:b52d]) } 13, 53 -- by smtp.googlemail.com with ESMTPSA id w133-v6sm526196itc.1.2018.03.31.11.48.02 } 13, 53 -- for {microscopy-at-microscopy.com} } 13, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 13, 53 -- Sat, 31 Mar 2018 11:48:03 -0700 (PDT) } 13, 53 -- Subject: Fwd: Dealing with old computers on microscopes } 13, 53 -- References: {CABq4i1Mrxt6jk8LuP0aFdJ4mo9QEK0dcw-bPYE1Fz49A0njdWg-at-mail.gmail.com} } 13, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 13, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 13, 53 -- X-Forwarded-Message-Id: {CABq4i1Mrxt6jk8LuP0aFdJ4mo9QEK0dcw-bPYE1Fz49A0njdWg-at-mail.gmail.com} } 13, 53 -- Message-ID: {ff74b986-ece8-2453-206d-43fdc3056019-at-gmail.com} } 13, 53 -- Date: Sat, 31 Mar 2018 13:48:02 -0500 } 13, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 13, 53 -- Gecko/20100101 Thunderbird/52.6.0 } 13, 53 -- MIME-Version: 1.0 } 13, 53 -- In-Reply-To: {CABq4i1Mrxt6jk8LuP0aFdJ4mo9QEK0dcw-bPYE1Fz49A0njdWg-at-mail.gmail.com} } 13, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 13, 53 -- Content-Language: en-US } 13, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers==============================
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Title-Subject: [Filtered] Issues with Aquila Topcon Hybrid SEM
Message: Hello. My name is Nareh and I am a PhD student at University of Southern California. We are currently having sample transfer issues with our Topcon SEM (Aquila Topcon Hybrid SEM) in the lab. Looks like there could be issues with the mechanical parts of the instrument that hold and transfer the sample holder to the stage. These are issues with loading and unloading the samples. We know this because most of the time the sample doesn't get loaded onto the stage and we are not able to see it in the OM map. Or if we get lucky and it gets loaded, we encounter issues with unloading the sample. The option to click on unload and vent the sample on the software is sometimes available and sometimes not. ( and when it is, the system just vents and comes back to atmospheric pressure without unloading the sample)
We tried doing some maintenance on the stage parts but haven't seen any improvements and the company we bought this SEM from hasn't been responding ( maybe out of business...)
We would really appreciate if you have any tips regarding these issues or if you could refer a company that may be able to fix this
Thanks
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We have a 1980's vintage Balzers freeze-fracture instrument (model 300/301?) and we are in search of someone who can service it. If you know of someone, or a company, I would appreciate getting some contact information.
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Email: johnshields59-at-gmail.com Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Bio TEM Workshop at GEM
Message: Biological TEM Workshop
This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and *hands-on* training led by GEM staff. What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Wednesday through Friday, July 25-27, 2018, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login Deadline: July 20, 2018
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Email: drademacher-at-luc.edu Name: David Rademacher
Organization: Loyola University Chicago
Title-Subject: [Filtered] ODP Circuit
Message: I came in this morning and observed the following error message on our Philips CM 120: ODP Circuit. Essentially what is happening is that, upon start-up, the system gets hung up at the very first step of the vacuum sequence. I am polling the expert audience to determine if anyone has encountered this issue and to learn what was done about it. Please don't hesitate to contact me drademacher-at-luc.edu with any comments/suggestions.
Best Regards,
Dave Rademacher Loyola University Chicago
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I challenged my son to make some Lego scopes about 20 years ago. Here are the results:
http://www.mta.ca/dmf/lego.html
Except for more appropriate stickers on the parts, I think they came out pretty well. Sadly, they often get more attention from visitors in the lab than the actual equipment does...
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
Hello Dave, In the Manual, there is written "ODP Circuit - contact service department".
We had this problem long time ago and we have solved it by buying a new ODP heater. We bought a new one from a company in Germany. Unfortunately, I do not have the details at hand now. The heater can be replaced by user. It is not a difficult task.
My best regards
Oldrich
P. S. Just now I have found the old broken heater ring from our Philips CM100. Its specification is "CHROMALOX CAT NO KB15 VOLTS 210 JB WATTS 450". The heater can be found on the EDWARDS Webshop:
Please, look at your diffusion pump type if this specification is also valid form your pump.
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
On Wed, 4 Apr 2018 18:42:40 -0500, microscopy.listserver-at-gmail.com wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } X-from: drademacher-at-luc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy both drademacher-at-luc.edu as well as the } Microscopy Listserver } --------------------------------------------------------------------------- } } Email: drademacher-at-luc.edu Name: David Rademacher } } Organization: Loyola University Chicago } } Title-Subject: [Filtered] ODP Circuit } } Message: I came in this morning and observed the following error } message on our Philips CM 120: ODP Circuit. Essentially what is } happening is that, upon start-up, the system gets hung up at the very } first step of the vacuum sequence. I am polling the expert audience } to determine if anyone has encountered this issue and to learn what } was done about it. Please don't hesitate to contact me } drademacher-at-luc.edu with any comments/suggestions. } } Best Regards, } } Dave Rademacher } Loyola University Chicago } } Login Host: 147.126.51.38 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original } Headers============================== 15, 53 -- From } microscopy.listserver-at-gmail.com Wed Apr 4 18:38:22 2018 15, 53 -- } Received: from mail-io0-f178.google.com (mail-io0-f178.google.com } [209.85.223.178]) 15, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id w34NcMKc015940 15, 53 -- } for {microscopy-at-microscopy.com} ; Wed, 4 Apr 2018 18:38:22 } -0500 15, 53 -- Received: by mail-io0-f178.google.com with SMTP id } o4so28389660iod.3 15, 53 -- for {microscopy-at-microscopy.com} ; } Wed, 04 Apr 2018 16:37:25 -0700 (PDT) 15, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 15, 53 -- d=gmail.com; } s=20161025; 15, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 15, 53 -- bh=o9KonzvKjas5xFx6necqI93eY5fDWDn3U96mV1V0w0U=; } 15, 53 -- } b=Tv3fFOfuRSILcKcHbmsqP0KJISsdif5DqwYn0R7UOVuYnjgNmaeyQ1aQLNlAGoK/rh } 15, 53 -- } id+L+U73moNG5REOODz/hDribnE7OnAwOVqo8o6ugEmbu7ov69n6SJh37IIv2Bj9aB4F } 15, 53 -- } YFjrIEy4Tfr250OjsTcqVuI3BRN0mk4nGsDQGxSgUvG6Ahsza+pYPhov3w7EA/WvUg6E } 15, 53 -- } aKph72NPGJbbl2pWn4PU3AupyPwSrEFwAB+jX7wo4cXVlsSQE57A4wNdkF8yM5nxZ5a2 } 15, 53 } -- /wa8ulE+hWWCt/HK5ex1pXakqGlZI6gjYKpukPvX1zzKrdYOizf67bbkuyDFVgqozxNf } 15, 53 -- D2rg== 15, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 15, 53 -- d=1e100.net; } s=20161025; 15, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 15, } 53 -- :user-agent:mime-version:in-reply-to:content-language } 15, 53 -- :content-transfer-encoding; 15, 53 -- } bh=o9KonzvKjas5xFx6necqI93eY5fDWDn3U96mV1V0w0U=; 15, 53 -- } b=e5zrVw5XPXA0TLst016JyctgnC8Mxt1Kgu9McrytVLyhSpOoIDZkJ+OK9Pv09XP0Gs } 15, 53 -- } 0DC8V9c6yIpoVPBcNKkpN7bTZLwsqtPPOCJXnke2FHBtigkI77wZgHqr4fcOP4K4haJ2 } 15, 53 -- } uMYEYh+7FsGPfk949+P4WKDtWbb8ulf+dBxkHRRS3u9vag37cfahHAYjG95UhZIIVvLw } 15, 53 -- } Bn8SfsVGFAq34oeOAQdQ5ivB8+Wtxf+fPgS7V3gkMlEZF1Q5FL2psQGlCxdBcPwOR/gx } 15, 53 -- } Hvao9IswohFg+onY34iTdW8R8qiaiWXSeVDbTEZJrFA1JiE3YlcRP2lF7PtzRSpYXwmx } 15, 53 -- ZtQw== 15, 53 -- X-Gm-Message-State: } ALQs6tARdxo2gbb3kHNDeFcXS+3PjlwisLppylc+pUccHmly1EfJKN2p 15, 53 -- } 78v/dWX/cAviiBmXQecmwWiigTeF 15, 53 -- X-Google-Smtp-Source: } AIpwx4+1rYJxuxt54yFGepovj6hiSLMqFtUnU10ra5duQXLJOHqit2bUTDUN3uxyfi0XAZsooVI5XA== } 15, 53 -- X-Received: by 10.107.168.78 with SMTP id } r75mr18348422ioe.143.1522885044555; 15, 53 -- Wed, 04 Apr } 2018 16:37:24 -0700 (PDT) 15, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:920:1d17:d99a:6454]) 15, 53 -- by } smtp.googlemail.com with ESMTPSA id } t10sm3952235ioa.29.2018.04.04.16.37.23 15, 53 -- for } {microscopy-at-microscopy.com} 15, 53 -- (version=TLS1_2 } cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 15, 53 -- } Wed, 04 Apr 2018 16:37:23 -0700 (PDT) 15, 53 -- Subject: viaWWW: ODP } Circuit Philips CM120 15, 53 -- References: } {201804041756.w34Hu0UC030639-at-microscopy.com} 15, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 15, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 15, 53 } -- X-Forwarded-Message-Id: } {201804041756.w34Hu0UC030639-at-microscopy.com} 15, 53 -- Message-ID: } {2492edf2-4b3e-e6bf-21a6-c712c1bd34b7-at-gmail.com} 15, 53 -- Date: Wed, } 4 Apr 2018 18:37:22 -0500 15, 53 -- User-Agent: Mozilla/5.0 } (Macintosh; Intel Mac OS X 10.11; rv:52.0) 15, 53 -- Gecko/20100101 } Thunderbird/52.7.0 15, 53 -- MIME-Version: 1.0 15, 53 -- In-Reply-To: } {201804041756.w34Hu0UC030639-at-microscopy.com} 15, 53 -- Content-Type: } text/plain; charset=windows-1252; format=flowed 15, 53 -- } Content-Language: en-US 15, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
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==============================Original Headers============================== 14, 44 -- From benada-at-biomed.cas.cz Thu Apr 5 07:33:34 2018 14, 44 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 14, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w35CXWUK011514 14, 44 -- for {microscopy-at-microscopy.com} ; Thu, 5 Apr 2018 07:33:33 -0500 14, 44 -- X-ASG-Debug-ID: 1522931553-05011e10f23e6a80001-4CH8be 14, 44 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id kZHY29IU6GxINSf0; Thu, 05 Apr 2018 14:32:33 +0200 (CEST) 14, 44 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 14, 44 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 14, 44 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 14, 44 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 14, 44 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 14, 44 -- (No client certificate requested) 14, 44 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id E5CAED01023; 14, 44 -- Thu, 5 Apr 2018 14:32:32 +0200 (CEST) 14, 44 -- Date: Thu, 5 Apr 2018 14:32:32 +0200 14, 44 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 14, 44 -- To: microscopy-at-microscopy.com, drademacher-at-luc.edu 14, 44 -- Subject: [Microscopy] viaWWW: ODP Circuit Philips CM120 14, 44 -- Message-ID: {20180405143232.4a524b34-at-u117ob02} 14, 44 -- X-ASG-Orig-Subj: [Microscopy] viaWWW: ODP Circuit Philips CM120 14, 44 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 14, 44 -- =?UTF-8?B?xIxS?= 14, 44 -- X-Mailer: Claws Mail 3.14.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 14, 44 -- MIME-Version: 1.0 14, 44 -- Content-Type: text/plain; charset=UTF-8 14, 44 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 14, 44 -- X-IoP-CAS-MailScanner-ID: E5CAED01023.AC103 14, 44 -- X-IoP-CAS-MailScanner: Processed 14, 44 -- X-Spam-Status: No 14, 44 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 14, 44 -- X-Barracuda-Start-Time: 1522931553 14, 44 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 14, 44 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 14, 44 -- X-Barracuda-Scan-Msg-Size: 7799 14, 44 -- X-Barracuda-BRTS-Status: 1 14, 44 -- X-Barracuda-Spam-Score: 0.50 14, 44 -- X-Barracuda-Spam-Status: No, SCORE=0.50 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=7.0 KILL_LEVEL=1000.0 tests=BSF_RULE7568M 14, 44 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.49608 14, 44 -- Rule breakdown below 14, 44 -- pts rule name description 14, 44 -- ---- ---------------------- -------------------------------------------------- 14, 44 -- 0.50 BSF_RULE7568M Custom Rule 7568M 14, 44 -- Content-Transfer-Encoding: 8bit 14, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w35CXWUK011514 ==============================End of - Headers==============================
Hi Dave, It sounds like a Water Chiller problem. Your chiller is probably making hot water, maybe good for tea but not for your microscope. Greg
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, April 4, 2018 7:52 PM To: Hendricks, Gregory {Gregory.Hendricks-at-umassmed.edu}
-------- Forwarded Message --------
X-from: Andy Stewart {andy.stewart1975-at-gmail.com}
Dear List-serv,
Here are the instructions to build a LEGO highbase Titan Themis we made for the launch of our microscope. https://ulsites.ul.ie/mssi/sites/default/files/Titan%20Themis%20Instructions.pdf {https://ulsites.ul.ie/mssi/sites/default/files/Titan Themis Instructions.pdf}
For those that are interested, here are the two microscopes being built. https://youtu.be/x6ArO0-CNu4
The launch video of the microscope https://youtu.be/PADirETav5k
To find those more difficult to source lego pieces I found bricklink.com {http://bricklink.com} to be very useful.
Happy Building Andy
———————————————————————————
Dr Andy Stewart Department of Physics & Energy Bernal Institute University of Limerick Ireland +353 (0) 61-23-7733 temul.ie {http://temul.ie}
Hi James and Lita I do need to point out that this competition is about making your smart phone into a microscope and needs to be a working model.
That being said: What a fun way to look at the instruments in your lab. Thank you for sharing James and thank you Lita.
Roseann Csencsits sent me this link https://ideas.lego.com/projects/fcce15cd-27e0-405b-990b-681b7787fbc6
I hope to see lots of wonderful models in Baltimore in August. Elaine
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada Lab: 250-853-3968 cell: 250-886-2068 website: http://www.stehm.uvic.ca
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-------- Forwarded Message -------- X-from: James M. Ehrman {jehrman-at-mta.ca} I challenged my son to make some Lego scopes about 20 years ago. Here are the results: http://www.mta.ca/dmf/lego.html Except for more appropriate stickers on the parts, I think they came out pretty well. Sadly, they often get more attention from visitors in the lab than the actual equipment does... Jim -- James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA phone: 506-364-2519 fax: 506-364-2505 email: jehrman-at-mta.ca www: http://www.mta.ca/dmf On 3/30/2018 7:32 PM, microscopy.listserver-at-gmail.com wrote: } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } -------- Forwarded Message -------- } } X-from: duraine-at-bcm.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } duraine-at-bcm.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: duraine-at-bcm.edu Name: Lita Duraine } } Organization: Howard Hughes Medical Institute } } Title-Subject: [Filtered] RE: [Microscopy] The Great Lego Microscope Competition } } Message: A LEGO microscope would be cool. } } In 2017 there was a push for the LEGO factory to make a LEGO Transmission Electron and Scanning } Electron Microscopes (not working of course). People could vote for it. Here is the web page: } https://ideas.lego.com/projects/102b2832-4574-4870-95a0-8698211d3fdf } } Not sure what happened to the idea, haven't seen any in the stores. But it would be neat to have in } the lab. } Lita } } Login Host: 128.249.1.198 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
X-from: John J. Perrino {jperrino-at-stanford.edu}
Hello all,
Our Leica UCT is having an issue, error code 102 with a U_15 in the FEED boxes, which will not allow me to turn the top lights on initially and now it also takes out the motor drive. I have localized the issue in the control box having replaced it with a twin Ultracut's control box and had no issues. The cable is also fine. Any suggestions would be greatly appreciated.
John
John Perrino Cell Sciences Imaging Facility 279 Campus Drive West B001 Beckman Stanford, CA 94305 650-723-3462 Fax 650-725-4951
X-from: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}
I used to look after two CMs until very recently. I think 'ODP circuit' means the heater's electric circuit is open (i.e. likely the heater element has failed, as someone else mentioned); 'ODP Water' is when the water temperature just after the ODP reaches 60 degrees C, i.e. water flow or chiller issue; 'ODP Oil' is triggered when the heater has been running for a while, but the oil hasn't reached target temperature- I've had this with a working heater but with a colder thank usual water supply at higher than normal flow rates; but it could also be an issue with the heater.
Ben
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Email: drademacher-at-luc.edu Name: David Rademacher
Organization: Loyola University Chicago
Title-Subject: [Filtered] ODP Circuit
Message: I came in this morning and observed the following error message on our Philips CM 120: ODP Circuit. Essentially what is happening is that, upon start-up, the system gets hung up at the very first step of the vacuum sequence. I am polling the expert audience to determine if anyone has encountered this issue and to learn what was done about it. Please don't hesitate to contact me drademacher-at-luc.edu with any comments/suggestions.
2) poor connection at ODP heater terminal (ceramic block located near bottom of ODP)
3) sticky ODP relay in MS unit (power cabinet, right side, one of large relays). Watch vacuum screen on data monitor during vacuum system startup. You must hear relay clicking simultaneously with ODP symbol highlighting shortly after vac. system start, as soon as P2 reading drops below 38. (assuming room is quiet)
4) defective water safety thermostat switch on water line coil (middle of ODP) or poor connection of it. Look at connecting wires, they can be burnt if touching bottom of ODP.
Other causes possible but unlikely
Vitaly Feingold SIA 2773 Heath Lane Duluth GA 30096 Ph. 770-232-7785 Fax 770-232-1791 www.sia-cam.com vitaly-at-sia-cam.com
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Essentially what is happening is that, upon start-up, the system gets hung up at the very } first step of the vacuum sequence. I am polling the expert audience to determine if anyone has } encountered this issue and to learn what was done about it. Please don't hesitate to contact me } drademacher-at-luc.edu with any comments/suggestions. } } Best Regards, } } Dave Rademacher } Loyola University Chicago } } Login Host: 147.126.51.38 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 15, 53 -- From microscopy.listserver-at-gmail.com Wed Apr 4 18:38:22 2018 } 15, 53 -- Received: from mail-io0-f178.google.com (mail-io0-f178.google.com [209.85.223.178]) } 15, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w34NcMKc015940 } 15, 53 -- for {microscopy-at-microscopy.com} ; Wed, 4 Apr 2018 18:38:22 -0500 } 15, 53 -- Received: by mail-io0-f178.google.com with SMTP id o4so28389660iod.3 } 15, 53 -- for {microscopy-at-microscopy.com} ; Wed, 04 Apr 2018 16:37:25 -0700 (PDT) } 15, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 53 -- d=gmail.com; s=20161025; } 15, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 15, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 15, 53 -- bh=o9KonzvKjas5xFx6necqI93eY5fDWDn3U96mV1V0w0U=; } 15, 53 -- b=Tv3fFOfuRSILcKcHbmsqP0KJISsdif5DqwYn0R7UOVuYnjgNmaeyQ1aQLNlAGoK/rh } 15, 53 -- id+L+U73moNG5REOODz/hDribnE7OnAwOVqo8o6ugEmbu7ov69n6SJh37IIv2Bj9aB4F } 15, 53 -- YFjrIEy4Tfr250OjsTcqVuI3BRN0mk4nGsDQGxSgUvG6Ahsza+pYPhov3w7EA/WvUg6E } 15, 53 -- aKph72NPGJbbl2pWn4PU3AupyPwSrEFwAB+jX7wo4cXVlsSQE57A4wNdkF8yM5nxZ5a2 } 15, 53 -- /wa8ulE+hWWCt/HK5ex1pXakqGlZI6gjYKpukPvX1zzKrdYOizf67bbkuyDFVgqozxNf } 15, 53 -- D2rg== } 15, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 15, 53 -- d=1e100.net; s=20161025; } 15, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 15, 53 -- :user-agent:mime-version:in-reply-to:content-language } 15, 53 -- :content-transfer-encoding; } 15, 53 -- bh=o9KonzvKjas5xFx6necqI93eY5fDWDn3U96mV1V0w0U=; } 15, 53 -- b=e5zrVw5XPXA0TLst016JyctgnC8Mxt1Kgu9McrytVLyhSpOoIDZkJ+OK9Pv09XP0Gs } 15, 53 -- 0DC8V9c6yIpoVPBcNKkpN7bTZLwsqtPPOCJXnke2FHBtigkI77wZgHqr4fcOP4K4haJ2 } 15, 53 -- uMYEYh+7FsGPfk949+P4WKDtWbb8ulf+dBxkHRRS3u9vag37cfahHAYjG95UhZIIVvLw } 15, 53 -- Bn8SfsVGFAq34oeOAQdQ5ivB8+Wtxf+fPgS7V3gkMlEZF1Q5FL2psQGlCxdBcPwOR/gx } 15, 53 -- Hvao9IswohFg+onY34iTdW8R8qiaiWXSeVDbTEZJrFA1JiE3YlcRP2lF7PtzRSpYXwmx } 15, 53 -- ZtQw== } 15, 53 -- X-Gm-Message-State: ALQs6tARdxo2gbb3kHNDeFcXS+3PjlwisLppylc+pUccHmly1EfJKN2p } 15, 53 -- 78v/dWX/cAviiBmXQecmwWiigTeF } 15, 53 -- X-Google-Smtp-Source: AIpwx4+1rYJxuxt54yFGepovj6hiSLMqFtUnU10ra5duQXLJOHqit2bUTDUN3uxyfi0XAZsooVI5XA== } 15, 53 -- X-Received: by 10.107.168.78 with SMTP id r75mr18348422ioe.143.1522885044555; } 15, 53 -- Wed, 04 Apr 2018 16:37:24 -0700 (PDT) } 15, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:920:1d17:d99a:6454]) } 15, 53 -- by smtp.googlemail.com with ESMTPSA id t10sm3952235ioa.29.2018.04.04.16.37.23 } 15, 53 -- for {microscopy-at-microscopy.com} } 15, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 15, 53 -- Wed, 04 Apr 2018 16:37:23 -0700 (PDT) } 15, 53 -- Subject: viaWWW: ODP Circuit Philips CM120 } 15, 53 -- References: {201804041756.w34Hu0UC030639-at-microscopy.com} } 15, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 15, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 15, 53 -- X-Forwarded-Message-Id: {201804041756.w34Hu0UC030639-at-microscopy.com} } 15, 53 -- Message-ID: {2492edf2-4b3e-e6bf-21a6-c712c1bd34b7-at-gmail.com} } 15, 53 -- Date: Wed, 4 Apr 2018 18:37:22 -0500 } 15, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 15, 53 -- Gecko/20100101 Thunderbird/52.7.0 } 15, 53 -- MIME-Version: 1.0 } 15, 53 -- In-Reply-To: {201804041756.w34Hu0UC030639-at-microscopy.com} } 15, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 15, 53 -- Content-Language: en-US } 15, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
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Email: holpc-at-firstenergycorp.com Name: Chris Holp
Organization: FirstEnergy - BETA Labs
Title-Subject: [Filtered] Vector graphics for images
Message: Hi everyone, We are interested in updating our photo editing software, with an eye primarily on good vector graphics for annotation purposes. I would appreciate receiving thoughts and suggestions; what do you use, what do you like about it, as well as what may be a little tricky/unsatisfactory (if anything) about your program.
Private replies are no problem, and thanks in advance! Chris Holp Staff Nuclear Specialist FirstEnergy BETA Lab 6670 Beta Dr. Mayfield Village, OH 44143 holpc-at-firstenergycorp.com
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Gatan, Inc. is the world's leading manufacturer of instrumentation and software used to enhance and extend the operation and performance of electron microscopes. The Gatan name is recognized and respected throughout the worldwide scientific community and has been synonymous with high quality products and the industry's leading technology.
We are currently seeking an experienced Technical Trainer/Instructor who will be responsible for organizing and delivering training for our global Service team. This individual will set standards for Service training, develop technical training curriculum based on company products and customer needs, and manage documentation and records. Some domestic and international travel will be involved with this role. This position will be based out of our Pleasanton, CA office.
The incumbent will be responsible for:
Devise technical training programs and certification tracking per organizational requirements. Produce training schedules and classroom agenda. Determine course content per objectives. Develop new training materials, which may include written documents, video tutorials, and on-line web based training. Arrange for and conduct on-site training when needed. Keep and report data on completed courses, issues etc. Observe and evaluate results of training programs. Determine overall effectiveness of programs and make improvements. Train support personnel such as tech support and field service engineers. Develop and document technical service procedures. Act as a two-way conduit for information transfer between the engineers who are responsible for designing our products and field service teams who are responsible for product maintenance, installation and support. Gain a systems-engineering level knowledge of highly complex, leading edge systems by working with the product engineering and service teams. The systems include optics, electronics, mechanical components, vacuum systems, custom software and algorithms.
The successful incumbent will have:
Experience in course development, training and documentation. Work on complex problems where analysis of situations or data requires an in-depth evaluation of various factors. Results-oriented and driven to excel. Enjoys teaching, troubleshooting, working with groups of people, and explaining complex things as simply as possible. Quick grasp of challenging, complex technical information.
Fundamental Requirements:
BS in Physics or Engineering (or equivalent) 5+ years related experience; Graduate degree a plus. Ability to successfully develop and facilitate technical training and documentation. Strong analytic and data analysis skills. Excellent oral and written communication skills. Ability to work effectively in multifunction, multicultural teams and to take a leadership role when needed. Strong communications and project management skills desired. Must have electronic, electromechanical, and vacuum skills in troubleshooting complex systems issues. Proven ability to service heavy equipment within acceptable ergonomic standards desired. Microscopy imaging/analytical equipment support experience preferred.
To Apply, log onto: https://recruiting.ultipro.com/ROP1001ROPER/JobBoard/d622ca39-91ce-4093-94dc-0dfda5adec2d/OpportunityDetail?opportunityId=b268136a-c81a-4e50-af94-aaa370b5a5fa
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractors legal duty to furnish information.
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Email: rburghardt-at-cvm.tamu.edu Name: Robert C Burghardt
Organization: Texas A&M University
Title-Subject: [Filtered] Research Associate Position - Transmission Electron Microscopy
Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences at Texas A&M University seeks a highly motivated individual to work in a state-of-the-art microscopy shared resource.
The individual must have extensive practical experience in biological sample preparation for transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of widefield fluorescence and confocal microscopy would also be a plus.
Excellent verbal and written communication skills and the ability to work with multiple users are essential. A doctoral degree in biology or related discipline is required.
Interested individuals should apply for this position via the Texas A&M University website https://tamus.wd1.myworkdayjobs.com/TAMU_External
Position number, R-003044
Applicants should include a resume along with a description of their practical expertise and contact information for 3 references.
Texas A&M University is and Equal Opportunity / Affirmative Action / Veterans / Disability Employer
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I find Adobe Illustrator to be my favorite choice. Although it costs money, everything is well-automated and there are tons of help websites, tutorials, and videos online, so that I never need more than a minute or two to figure out a new command. The ability to do my annotations FAST more than makes up for the yearly cost of the Adobe CC subscription, given my hourly chargeout rate. If you want free, Inkscape seems good, but I've not bothered to learn it in detail.
I do all of my analysis in MATLAB and use commands like } save( gcf,'filename.pdf','pdf' ); to save my MATLAB figures as PDF, which Illustrator will read natively as vector graphics. That's the fastest, most efficient way to turn data into figures I've found. I imagine Python, etc., have similar tricks using either PDF or SVG.
(No financial interest -- just a satisfied customer.)
Chad
--------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Team Nuclear Materials Science and Technology Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, April 06, 2018 8:32 AM To: Parish, Chad M.
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Email: holpc-at-firstenergycorp.com Name: Chris Holp
Organization: FirstEnergy - BETA Labs
Title-Subject: [Filtered] Vector graphics for images
Message: Hi everyone, We are interested in updating our photo editing software, with an eye primarily on good vector graphics for annotation purposes. I would appreciate receiving thoughts and suggestions; what do you use, what do you like about it, as well as what may be a little tricky/unsatisfactory (if anything) about your program.
Private replies are no problem, and thanks in advance! Chris Holp Staff Nuclear Specialist FirstEnergy - BETA Lab 6670 Beta Dr. Mayfield Village, OH 44143 holpc-at-firstenergycorp.com
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I am looking for a complete Gatan PIPS 691, functional or non-functional, or parts for non-commercial use. I would be happy to pay a small amount of money and of course for packing and sending.
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: vera.desmarais-at-einstein.yu.edu Name: Vera DesMarais
Organization: Analytical Imaging facility Einstein College of Medicine Title-Subject: [Filtered] Job Opportunity
Message: The Analytical Imaging Facility (Microscopy Core facility) at Albert Einstein College of Medicine in New York is currently looking for an entry level light microscopy technician. Please follow the link below for a more detailed job description and to apply for the job.
I wanted to know if it is possible to get difference in electronic properties, such as work function (potential needed to extract an electron), between two materials of similar dimensions, from exit wave reconstruction.
i.e. suppose I have 2 nano particles, of radius r, both are identical as such but one have slightly higher wrok function. Can i visualize that work function difference using exit wave? I am not able to find exact literature. How ever several tantalizingly close results exist. such as this- pubs.acs.org/doi/abs/10.1021/acs.nanolett.6b04957
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Email: patrick.smith-at-beg.utexas.edu Name: patrick smith
Organization: BEG
Title-Subject: [Filtered] riggers to move SEM
Message: We're getting ready to move to a new facility. I like to know of any companies that specialize moving SEM's. The instrument will be in moving condition.
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Title-Subject: [Filtered] Summer Courses at EMS Microscopy Academy
Message: Microscopy: The Complete Image (3 weeks) Add to your skills in Scanning, Transmission, Optical Light, or General Microscopy! Walk away in three short weeks with the ability to run and be proficient in all aspects of specimen preparation and biological materials. Whether you are beginning in microscopy or advanced, our classes are customized to cater to your individual skill set. Come join us! Be a glowing asset in any lab you walk into!
We are offering three options this summer:
June Session- June 11 - 29, 2018 8:30 a.m. - 5:00 p.m. Hatfield, Pennsylvania, USA
July Session- July 9 - 27, 2018 8:30 a.m. - 5:00 p.m. Hatfield, Pennsylvania, USA
August Session- August 6 - 24, 2018 8:30 a.m. - 5:00 p.m. Hatfield, Pennsylvania, USA
Copy and paste this link into your browser to sign up today: https://www.emsdiasum.com/microscopy/academy/courses/summer.aspx
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Email: jettsd-at-nih.gov Name: Stephen Jett
Organization: National Cancer Institute
Title-Subject: [Filtered] NCI Request for Information on development of an Imaging Data Commons
Message: Hello everyone,
As part of its efforts to increase access to cancer data and data analysis tools, the National Cancer Institute is establishing a Cancer Research Data Commons (CRDC). Imaging is a crucial area for analysis and discovery in cancer, so part of this effort is the development of an Imaging Data Commons (IDC). Given the breadth of the data and tools in cancer imaging, we are seeking input from the research community to ask their thoughts on the data, tools and features they feel are important to include in such a data commons. To engage potential contributors, a Request for Information (RFI) was released to solicit this input. Ive included below the text of a flyer were distributing to announce the RFI, including the URL to the RFI and the email for questions/responses. You can also contact me directly with any questions. If you are aware of similar existing efforts, please feel free to reference those in a response. If you have any questions regarding the RFI, you may send them to either the NCIIDCRFI-at-mail.nih.gov address or jettsd-at-nih.gov (theyre both me). Thank you in advance for any input. Best regards, Steve Jett The NCI is inviting comments and suggestions on the development of the NCI Imaging Data Commons (IDC), a node of the Cancer Research Data Commons. The IDC will provide: · access to image repositories · analysis tools · scalable computing resource · a cloud-based, collaborative environment. To best serve the needs of the cancer imaging community, we are seeking input from potential users of the IDC to determine the best features to include in an IDC prototype. All stakeholders involved in cancer imaging are invited to respond to this Request. More details about the RFI and how to respond can be found at https://grants.nih.gov/grants/guide/notice-files/NOT-CA-18-060.html The deadline for submission is May 4, 2018.
For any questions about this request, please contact
NCIIDCRFI-at-mail.nih.gov ___________________ Stephen D. Jett, Ph.D. AAAS Science & Technology Policy Fellow Center for Biomedical Informatics and Information Technology (CBIIT) National Cancer Institute, National Institutes of Health 9609 Medical Center Drive Rockville, MD 20850 Tel.: 240-276-5537 stephen.jett-at-nih.gov
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I am sorry to report that my friend and mentor, Caroline Schooley, passed away early Sunday morning with family members at her side.
When I was a new grad student in 1976, working in a lab looking at neurosecretion, mostly with electrophysiology, it was suggested to my advisor that someone learn electron microscopy for the project. So in 1976 I got 'volunteered' and was sent to Berkeley for a summer course led by Caroline Schooley. At first I was overwhelmed and terrified, but within a couple of weeks Caroline, with her unique blend of generosity and gruffness, had gotten me into a better housing situation, introduced me to people who would become lifelong friends, and fueled my fascination with microscopy. It didn't take long for me to decide I wanted to make EM a career, and also that it might be both fun and challenging to run a multi-user facility like hers where people from all walks of academia, from science to art, could meet and exchange ideas. She insisted I join MSA early on, pointing out that the Society was unusual in being gender blind and also degree blind, welcoming to everyone. Over the years I also spent time with her family in Berkeley and Mendocino as well as in Kona, surrounded by literally stacks and stacks of books, enjoying food and wine, tidepooling, and photography. I used to be shy and reserved, and uncertain of my place because I was isolated (in those days) on a small island in the middle of the Pacific. But Caroline encouraged me to become involved with and serve the Society. This drew me out and gave me a better sense of self, and no one who knows me can say I'm shy anymore. Each of us has special people in our lives who have helped to guide us to where we are. For me, this person was Caroline Schooley.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 4, 20 -- From tina-at-pbrc.hawaii.edu Sun Apr 15 18:39:07 2018 4, 20 -- Received: from b1000.pbrc.hawaii.edu (b1000.pbrc.hawaii.edu [128.171.22.30]) 4, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w3FNd4DD014591 4, 20 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Apr 2018 18:39:05 -0500 4, 20 -- Received: from b1000.pbrc.hawaii.edu (localhost [127.0.0.1]) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5) with ESMTP id w3FNcg2e029028 4, 20 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-SHA bits=256 verify=NO) 4, 20 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Apr 2018 13:38:42 -1000 (HST) 4, 20 -- Received: from localhost (tina-at-localhost) 4, 20 -- by b1000.pbrc.hawaii.edu (8.13.5/8.13.5/Submit) with ESMTP id w3FNcfkr029024 4, 20 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Apr 2018 13:38:42 -1000 (HST) 4, 20 -- X-Authentication-Warning: b1000.pbrc.hawaii.edu: tina owned process doing -bs 4, 20 -- Date: Sun, 15 Apr 2018 13:38:41 -1000 (HST) 4, 20 -- From: Tina Carvalho {tina-at-pbrc.hawaii.edu} 4, 20 -- X-X-Sender: tina-at-b1000 4, 20 -- To: Microscopy Listserver {Microscopy-at-microscopy.com} 4, 20 -- Subject: About Caroline Schooley 4, 20 -- Message-ID: {Pine.GSO.4.64.1804151159170.27924-at-b1000} 4, 20 -- MIME-Version: 1.0 4, 20 -- Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed ==============================End of - Headers==============================
When I read about how Caroline mentored Tina, it struck me that was what she did for me too. I met Caroline in the early 2000's at M&M as I was interested in doing more Outreach at home. She had retired from running a busy lab at Berkeley and put a lot of energy into getting ProjectMICRO started. She supported the MSA collaboration with the Lawrence Hall of Science to publish the GEMS book "Microscopic Explorations." When she found it difficult to come to the meetings I agreed to co-chair ProjectMICRO and look after the meeting side. At that time, I knew I did not know enough to do the job properly and it was good that she "had my back." She made sure reports went in on time, boxes of materials for ProjectMICRO arrived on time, I was on time. She knew how to get things done and mentored me along the way. At first, she could seem gruff and maybe intimidating if someone was not listening to her, but her heart was in the right place and it was golden and she was always generous. She knew how things should be done. I will miss her. Elaine
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada
I am sorry to report that my friend and mentor, Caroline Schooley, passed away early Sunday morning with family members at her side.
When I was a new grad student in 1976, working in a lab looking at neurosecretion, mostly with electrophysiology, it was suggested to my advisor that someone learn electron microscopy for the project. So in 1976 I got 'volunteered' and was sent to Berkeley for a summer course led by Caroline Schooley. At first I was overwhelmed and terrified, but within a couple of weeks Caroline, with her unique blend of generosity and gruffness, had gotten me into a better housing situation, introduced me to people who would become lifelong friends, and fueled my fascination with microscopy. It didn't take long for me to decide I wanted to make EM a career, and also that it might be both fun and challenging to run a multi-user facility like hers where people from all walks of academia, from science to art, could meet and exchange ideas. She insisted I join MSA early on, pointing out that the Society was unusual in being gender blind and also degree blind, welcoming to everyone. Over the years I also spent time with her family in Berkeley and Mendocino as well as in Kona, surrounded by literally stacks and stacks of books, enjoying food and wine, tidepooling, and photography. I used to be shy and reserved, and uncertain of my place because I was isolated (in those days) on a small island in the middle of the Pacific. But Caroline encouraged me to become involved with and serve the Society. This drew me out and gave me a better sense of self, and no one who knows me can say I'm shy anymore. Each of us has special people in our lives who have helped to guide us to where we are. For me, this person was Caroline Schooley.
Aloha, Tina
**************************************************************************** * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu * * Biological Electron Microscope Facility * (808) 956-6251 * * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf* ****************************************************************************
==============================Original Headers============================== 8, 51 -- From ech-at-uvic.ca Sun Apr 15 19:25:40 2018 8, 51 -- Received: from dugong.comp.uvic.ca (dugong.comp.uvic.ca [142.104.42.98]) 8, 51 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w3G0PcXA015339 8, 51 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Apr 2018 19:25:39 -0500 8, 51 -- Received: from madtom.uvic.ca (madtom.uvic.ca [142.104.224.129]) 8, 51 -- by dugong.comp.uvic.ca (8.14.4/8.14.4) with ESMTP id w3G0PGaO012347 8, 51 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES256-GCM-SHA384 bits=256 verify=FAIL) 8, 51 -- for {Microscopy-at-microscopy.com} ; Sun, 15 Apr 2018 17:25:16 -0700 8, 51 -- Received: from lyretail.uvic.ca (142.104.225.129) by madtom.uvic.ca 8, 51 -- (142.104.224.129) with Microsoft SMTP Server (TLS) id 15.0.1365.1; Sun, 15 8, 51 -- Apr 2018 17:25:15 -0700 8, 51 -- Received: from lyretail.uvic.ca ([fe80::a175:af10:b91b:4534]) by 8, 51 -- lyretail.uvic.ca ([fe80::a175:af10:b91b:4534%13]) with mapi id 8, 51 -- 15.00.1365.000; Sun, 15 Apr 2018 17:25:15 -0700 8, 51 -- From: Elaine Humphrey {ech-at-uvic.ca} 8, 51 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 51 -- Subject: Re: [Microscopy] About Caroline Schooley 8, 51 -- Thread-Topic: [Microscopy] About Caroline Schooley 8, 51 -- Thread-Index: AQHT1RV8zrgctqGLykCmfSlINmwzlqQCiJyA 8, 51 -- Date: Mon, 16 Apr 2018 00:25:15 +0000 8, 51 -- Message-ID: {7085D3AA-CA62-4CDB-A21C-AD53647D4856-at-uvic.ca} 8, 51 -- References: {201804152357.w3FNvbKr031024-at-microscopy.com} 8, 51 -- In-Reply-To: {201804152357.w3FNvbKr031024-at-microscopy.com} 8, 51 -- Accept-Language: en-US 8, 51 -- Content-Language: en-US 8, 51 -- X-MS-Has-Attach: 8, 51 -- X-MS-TNEF-Correlator: 8, 51 -- user-agent: Microsoft-MacOutlook/10.b.0.180311 8, 51 -- x-ms-exchange-messagesentrepresentingtype: 1 8, 51 -- x-ms-exchange-transport-fromentityheader: Hosted 8, 51 -- x-originating-ip: [142.104.193.193] 8, 51 -- x-tm-as-product-ver: SMEX-12.0.0.1220-8.200.1013-23786.003 8, 51 -- x-tm-as-result: No--18.688000-8.000000-31 8, 51 -- x-tm-as-matchedid: 150567-701625-704425-700685-703788-704083-701450-710096-1 8, 51 -- 21158-708136-704990-702213-706023-707565-701674-702190-701128-701921-705450 8, 51 -- -704561-708212-702444-708863-700963-187125-700492-187014-139704-708196-7000 8, 51 -- 75-187067-700949-700345-702187-390312-704341-701346-705584-704102-702990-70 8, 51 -- 1298-711956-706249-700756-840032-703212-121116-701310-701529-706527-105400- 8, 51 -- 702613-702920-700838-701907-706891-700617-700752-706119-703041-705492-18819 8, 51 -- 8-148004-148133-20020-42000-42003 8, 51 -- x-tm-as-user-approved-sender: No 8, 51 -- x-tm-as-user-blocked-sender: No 8, 51 -- Content-Type: text/plain; charset="utf-8" 8, 51 -- Content-ID: {1BDC3ADB94707342AB89178CF88CE454-at-local.uvic.ca} 8, 51 -- MIME-Version: 1.0 8, 51 -- X-UVic-Virus-Scanned: OK - Passed virus scan by ClamAV (clamd) on dugong 8, 51 -- X-UVic-Virus-Scanned: OK - Passed virus scan by Sophos (sophie) on dugong 8, 51 -- X-UVic-Scan: dugong.comp.uvic.ca filter_version 3.7.9 8, 51 -- X-Scanned-By: MIMEDefang 2.78 8, 51 -- Content-Transfer-Encoding: 8bit 8, 51 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w3G0PcXA015339 ==============================End of - Headers==============================
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: George Washington University
Title-Subject: [Filtered] Chesapeake Society Spring Meeting
Message: CMMS meeting: May 8th, 5 - 8pm, George Washington University Dear CMMS members, Please join us for a dinner meeting on May 8th at the George Washington University. Our speaker that evening will be Dr. Shigeki Watanabe who's talk is entitled "Ultrafast endocytosis of synaptic vesicles". Dr. Watanabe's talk will be preceded by dinner and a short business meeting. A printable flyer for the meeting is available here.
The cost for the meeting will be $35 dollars per person. Registration is available here. The registration deadline is May 4th; we regret that we cannot accept registrations after that date or at the door. The business meeting will be particularly important as there are several issues that must be decided: The Society needs someone to assume the duties (regulatory and tax filings) of the resident agent as I will be leaving the DC/MD/NoVA area this summer. This must be a Maryland resident as the Society is currently incorporated in Maryland. The Society needs at least two people who are interested in helping to organize meetings and events for the Society to step up and volunteer their time. Without both of these matters being decided, the future of the Society will be in question so I urge you to attend this meeting and become involved in the future of the Society.
If you have any questions, please contact me at ChesapeakeMicroscopy-at-gmail.com
I look forward to seeing many of you on the 8th.
Sincerely, Cam Robinson President, CMMS
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Dear Colleagues, please distribute among your students, postdocs and associates. Thank you Andrei
++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ NIST (PNL/CNST) is expanding its research program toward the development of in situ electron microscopy (SEM, PEEM etc) and spectroscopy (XPS, AES, XAS etc) of objects/processes (incl. bio/medical) and devices in liquids and dense gaseous environments using environmental cells equipped with electron transparent windows (e.g. graphene). Currently, a postdoctoral position for highly motivated and experienced experimentalist is available. The preferable set of skills includes, but not limited to SEM, XPS, AES, PEEM, AFM, u-Raman, CL, UHV surface science techniques, synchrotron radiation and clean room experience, graphene transfer/ functionalization protocols, electrochemistry, excellent writing and teamwork skills and etc. The application letter, CV with publication record and contact names of 3-4 references should be sent to Dr. Andrei Kolmakov, andrei.kolmakov-at-nist.gov In case further information is needed, do not hesitate to contact Dr. Andrei Kolmakov at:
NIST 100 Bureau Drive Bldg. 216/Rm.B117 Gaithersburg, MD 20899-6204 Phone: (301) 975-4724 Fax: (301) 975-2303 +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ ___________________________________________________ Dr. Andrei Kolmakov Project Leader, Nanoscale Imaging and Spectroscopy Group Center for Nanoscale Science & Technology National Institute of Standards & Technology 100 Bureau Dr. Mail Stop 6203 Gaithersburg, MD 20899 Phone: (301) 975-4724 Fax: (301) 975-2303 Email: andrei.kolmakov-at-nist.gov URL https://www.nist.gov/people/andrei-kolmakov
Richard Francis Earl Crang (81) passed away on April 13, 2018 at his home in La Grange Highlands, IL. He is survived by his wife Mary; two sons, Steven (Diane) and Douglas (Kate); four grandsons, Alexander, Eliot, Evan and Nathaniel; and three adult step-sons. He was a graduate of Eastern Illinois University, and received his masters degree at the University of South Dakota and his Ph.D. in Botany at the University of Iowa. He later carried out a year of post-doctoral work in Botany and Physiology at Clare Hall of the University of Cambridge, England. He held academic positions at Wittenberg University (Springfield, OH), Bowling Green State University (OH), the University of Illinois at Urbana-Champaign (UIUC), and The City University of New York. At UIUC, he served for 12 years as the Director of the Center for Electron Microscopy; two years as Associate Head of the Department of Plant Biology; and over two years as a Faculty Fellow in the Office of the Vice President for Academic Affairs. Dr. Crang received an Alfred P. Sloan Foundation Fellowship to support his pioneering work in the development of an online course and electronic textbook in Plant Anatomy. He later guided development of the first online academic courses in Nursing and Pharmacy at the University of Illinois. He served for several years as a member and Chair of the National Research Council graduate fellowship committee for the National Science Foundation. He was sponsored by the Federal EPA and the US Department of State to carry out research work at the Komarov Botanical Institute in St. Petersburg (formerly Leningrad) Russia during the 1980s. He also held residential research appointments in microscopy at the Max Planck Institute for Molecular Physiology in Dortmund, Germany and at the Swiss Federal Institute of Technology in Zurich, Switzerland. Dr. Crang is the only U.S. Plant Biologist to be awarded Fellowship status by the International Society of Environmental Botanists. While at Bowling Green State University, he was the first recipient of the Universitys Outstanding Research Award. Later, Dr. Crang was named the second recipient of the Outstanding Service Award of the Electron Microscopy Society of America. He has been the author/co-author of six textbooks, over 70 academic papers, and over one hundred abstracts, short papers and submitted contributions in the fields of microscopy and plant biology. His most recent work has been as co-author for a just-completed comprehensive textbook on Plant Anatomy. As Professor Emeritus, he carried out humanitarian missionary work in Colombia and was on the instructional faculty at the Pyongyang University of Science and Technology in North Korea. Relentlessly curious, he traveled to 41 countries where he eagerly engaged with local people and enthusiastically explored local cultures. Visitation with the family will be Tuesday, April 17, 2018 from 1:00 P.M. to time of service 2 P.M. at Hitzeman Funeral Home & Cremation Services, 9445 W. 31st St., Brookfield, Illinois 60513. In lieu of flowers, the family asks that donations be made to the Plant Biology Annual Fund in the Dept. of Plant Biology at UIUC in Dr. Crangs name at sib.illinois.edu/alumni/donations or Department of Plant Biology, 265 Morrill Hall, MC-116, 505 South Goodwin Avenue, Urbana, IL 61801. Information 708-485-2000 or www.HitzemanFuneral.com
{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } } Lou Ann Miller Biological Electron Microscopy Frederick Seitz Materials Research Laboratory Lab Room 125 Office 374 MRL 104 South Goodwin Ave Urbana, IL 61801 Campus mail code: MC-230 Work Hours: 7am - 3:30 pm
Can I please get your advice on a problem with the HT on a Philips CM100?
When I press the HT On/Off button to turn on the high tension, I got no response, i.e., I cannot turn on the high tension. The vacuum are good, both HiV and UHV lights are on. I pulled the power supply (A7) and found that the fuses are intact. The voltage going into A7 is 227V. I also measured the resistance across the HT On/Off push button. It is 12 ohm when pressed and 0 ohm released.
Is there anything obvious that I have missed? Thanks!
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (http://depts.washington.edu/if/)
Hello Pang, There is a security switch "S34" on CM100. You can find it on the left side of the column on the lifting assembly. Check it if it is closed. If it is open, the HT cannot be switched on.
Regards
Oldrich
-- Oldřich Benada Institute of Microbiology CAS, v.v.i. Laboratory of Molecular Structure Characterization Vídeňská 1083 142 20 Prague 4 Czech Republic
On Thu, 19 Apr 2018 23:58:44 -0500, wpchan-at-uw.edu wrote : } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi } } Can I please get your advice on a problem with the HT on a Philips } CM100? } } When I press the HT On/Off button to turn on the high tension, I got } no response, i.e., I cannot turn on the high tension. The vacuum are } good, both HiV and UHV lights are on. I pulled the power supply (A7) } and found that the fuses are intact. The voltage going into A7 is } 227V. I also measured the resistance across the HT On/Off push } button. It is 12 ohm when pressed and 0 ohm released. } } Is there anything obvious that I have missed? Thanks! } } } -- } Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) } The Biology Imaging Facility (http://depts.washington.edu/if/) } } ==============================Original } Headers============================== 6, 41 -- From wpchan-at-uw.edu Thu } Apr 19 23:52:10 2018 6, 41 -- Received: from mxout25.s.uw.edu } (mxout25.s.uw.edu [140.142.234.175]) 6, 41 -- by } microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w3K4qAwV020136 } 6, 41 -- for {microscopy-at-microscopy.com} ; Thu, 19 Apr 2018 } 23:52:10 -0500 6, 41 -- Received: from mail-oi0-f70.google.com } (mail-oi0-f70.google.com [209.85.218.70]) 6, 41 -- by } mxout25.s.uw.edu (8.14.4+UW14.03/8.14.4+UW16.03) with ESMTP id } w3K4mtqv020767 6, 41 -- (version=TLSv1/SSLv3 } cipher=AES128-GCM-SHA256 bits=128 verify=OK) 6, 41 -- for } {microscopy-at-microscopy.com} ; Thu, 19 Apr 2018 21:48:56 -0700 6, 41 -- } Received: by mail-oi0-f70.google.com with SMTP id } q83-v6so3964937oif.2 6, 41 -- for } {microscopy-at-microscopy.com} ; Thu, 19 Apr 2018 21:48:56 -0700 (PDT) 6, } 41 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 41 -- d=1e100.net; s=20161025; 6, 41 -- } h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, } 41 -- bh=jZvAPENgPspI9RxmybefDJV3ofd+hf0nPK3uzFn5D4k=; 6, 41 } -- } b=Def/qLf7AiJkYi3nbYLfWSabUdbpGtVAA5w9B3qQcLKZtmg4Xy1aWF4qi2ov2AS9RJ } 6, 41 -- } 1P7LTbCbwxhsSkOjOdzqTOV3n+lQludDHmeIDiWTmBlzL00UVzWveLa+uni+VmC2hsx7 } 6, 41 -- } R9gddqQP6xzp5IJP/gZR9qTIfMuAkQigul/i/7LcD7wv/l20S8WHaoKkQ+SiK3M2+XbP } 6, 41 -- } zu3imE2lkXwI6VkKPp+w8mtxxjmuw9NqF0Ck5Rw95PPJFsvyhPHkj3JHDqBUN5X3i6NB } 6, 41 -- } VXqHR2aZr3e+sjlZIzFWdtQNteU2X8upQLAt+zS5LkSvuYofOxSEQ2adPrfQSnqKdEKg } 6, 41 -- +oew== 6, 41 -- X-Gm-Message-State: } ALQs6tA69Rk0ZSK7UtPvACx6zQkVDRxc+iu+V3rQF6Mgm2DaTGA66AKp 6, 41 -- } bQnhlWrYlDNx4vzB+P10aRAke6bhsZx94zD4u+RpRhhGMa+xlikEkcPS8NWknx0n99mWYt4m+j+ } 6, 41 -- } 76JwN1lgOS3b+Yy3/eTK4uSgLaATIuOraIM1SSeUTC6NuQT6PFJ5quoLthR1iAg== 6, } 41 -- X-Received: by 2002:a9d:72d0:: with SMTP id } d16-v6mr5337782otk.83.1524199735032; 6, 41 -- Thu, 19 Apr } 2018 21:48:55 -0700 (PDT) 6, 41 -- X-Google-Smtp-Source: } AIpwx49gVqEemSMBL76LeKHwVcMcx8XNYtWAM25vY+uyJz8AcF5jsrQpKPYPvKIifP0VZMX3TrBdYhm0bJ8PN90Csuc= } 6, 41 -- X-Received: by 2002:a9d:72d0:: with SMTP id } d16-v6mr5337775otk.83.1524199734679; 6, 41 -- Thu, 19 Apr 2018 } 21:48:54 -0700 (PDT) 6, 41 -- MIME-Version: 1.0 6, 41 -- Received: by } 2002:a9d:4005:0:0:0:0:0 with HTTP; Thu, 19 Apr 2018 21:48:54 6, 41 } -- -0700 (PDT) 6, 41 -- From: Wai Pang Chan {wpchan-at-uw.edu} 6, 41 -- } Date: Thu, 19 Apr 2018 21:48:54 -0700 6, 41 -- Message-ID: } {CALbJ0i7Z-PD_A_346cn0WDF7oeXVw5QV+h=q44AnhFk3Qiorpg-at-mail.gmail.com} } 6, 41 -- Subject: HT on a Philips CM100 6, 41 -- To: } microscopy-at-microscopy.com 6, 41 -- Content-Type: text/plain; } charset="UTF-8" 6, 41 -- X-PMX-Version: 6.3.3.2656215, } Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2018.4.20.43916, } AntiVirus-Engine: 5.49.1, AntiVirus-Data: 2018.4.19.5491002 6, 41 -- } X-PMX-Server: mxout25.s.uw.edu 6, 41 -- X-Uwash-Spam: Gauge=IIIIIIII, } Probability=8%, Report= 6, 41 -- HTML_00_01 0.05, HTML_00_10 0.05, } BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1000_LESS 0, } BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_600_699 0, } BODY_SIZE_7000_LESS 0, CT_TEXT_PLAIN_UTF8_CAPS 0, DATE_TZ_NA 0, } FROM_EDU_TLD 0, FROM_NAME_PHRASE 0, NO_URI_HTTPS 0, } SINGLE_URI_IN_BODY 0, SPF_NEUTRAL 0, URI_WITH_PATH_ONLY 0, } WEBMAIL_SOURCE 0, __ANY_URI 0, __C230066_P5 0, __CP_URI_IN_BODY 0, } __CT 0, __CT_TEXT_PLAIN 0, __DQ_NEG_HEUR 0, __DQ_NEG_IP 0, __HAS_FROM } 0, __HAS_MSGID 0, __HELO_GMAIL 0, __MIME_TEXT_ONLY 0, __MIME_TEXT_P } 0, __MIME_TEXT_P1 0, __MIME_VERSION 0, __NO_HTML_TAG_RAW 0, } __PHISH_SPEAR_HTTP_RECEIVED 0, __PHISH_SPEAR_STRUCTURE_1 0, } __PHISH_SPEAR_STRUCTURE_2 0, __RDNS_GMAIL 0, __SANE_MSGID 0, } __SINGLE_URI_TEXT 0, __SUBJ_ALPHA_END 0, __TO_MALFORMED_2 0, } __TO_NO_NAME 0, __URI_IN_BODY 0, __URI_NOT_IMG 0, __URI_NO_WWW 0, } __URI_NS , __URI_WITH_PATH 0, __YOUTUBE_RCVD 0 } ==============================End of - } Headers==============================
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==============================Original Headers============================== 10, 47 -- From benada-at-biomed.cas.cz Fri Apr 20 01:15:40 2018 10, 47 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 10, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w3K6FdMK026169 10, 47 -- for {microscopy-at-microscopy.com} ; Fri, 20 Apr 2018 01:15:40 -0500 10, 47 -- X-ASG-Debug-ID: 1524204929-05011e10ef5d9630001-4CH8be 10, 47 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id TMhvA8OtfsVRhrBD; Fri, 20 Apr 2018 08:15:29 +0200 (CEST) 10, 47 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 10, 47 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 10, 47 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 10, 47 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 47 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 10, 47 -- (No client certificate requested) 10, 47 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id AB3F6D004AF; 10, 47 -- Fri, 20 Apr 2018 08:15:29 +0200 (CEST) 10, 47 -- Date: Fri, 20 Apr 2018 08:15:27 +0200 10, 47 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 47 -- To: wpchan-at-uw.edu, {microscopy-at-microscopy.com} 10, 47 -- Subject: Re: [Microscopy] HT on a Philips CM100 10, 47 -- Message-ID: {20180420081527.5aa95ea0-at-u117ob02} 10, 47 -- X-ASG-Orig-Subj: Re: [Microscopy] HT on a Philips CM100 10, 47 -- In-Reply-To: {201804200458.w3K4wiYt023372-at-microscopy.com} 10, 47 -- References: {201804200458.w3K4wiYt023372-at-microscopy.com} 10, 47 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 47 -- =?UTF-8?B?xIxS?= 10, 47 -- X-Mailer: Claws Mail 3.14.1 (GTK+ 2.24.25; i586-pc-linux-gnu) 10, 47 -- MIME-Version: 1.0 10, 47 -- Content-Type: text/plain; charset=UTF-8 10, 47 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 47 -- X-IoP-CAS-MailScanner-ID: AB3F6D004AF.ACE4B 10, 47 -- X-IoP-CAS-MailScanner: Processed 10, 47 -- X-Spam-Status: No 10, 47 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 10, 47 -- X-Barracuda-Start-Time: 1524204929 10, 47 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 10, 47 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 10, 47 -- X-Barracuda-Scan-Msg-Size: 6642 10, 47 -- X-Barracuda-BRTS-Status: 1 10, 47 -- X-Barracuda-Spam-Score: 1.00 10, 47 -- X-Barracuda-Spam-Status: No, SCORE=1.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=7.0 KILL_LEVEL=1000.0 tests=BSF_RULE7568M, BSF_RULE_7582B 10, 47 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.50087 10, 47 -- Rule breakdown below 10, 47 -- pts rule name description 10, 47 -- ---- ---------------------- -------------------------------------------------- 10, 47 -- 0.50 BSF_RULE_7582B Custom Rule 7582B 10, 47 -- 0.50 BSF_RULE7568M Custom Rule 7568M 10, 47 -- Content-Transfer-Encoding: 8bit 10, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w3K6FdMK026169 ==============================End of - Headers==============================
Thank you everyone for all the helpful tips. I'll try them out and report back. Have a great weekend.
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (http://depts.washington.edu/if/)
} On Thu, 19 Apr 2018 23:58:44 -0500, wpchan-at-uw.edu wrote :
} } Hi } } } } Can I please get your advice on a problem with the HT on a Philips } } CM100? } } } } When I press the HT On/Off button to turn on the high tension, I got } } no response, i.e., I cannot turn on the high tension. The vacuum are } } good, both HiV and UHV lights are on. I pulled the power supply (A7) } } and found that the fuses are intact. The voltage going into A7 is } } 227V. I also measured the resistance across the HT On/Off push } } button. It is 12 ohm when pressed and 0 ohm released. } } } } Is there anything obvious that I have missed? Thanks!
From alex-at-hyipme.com Sat Apr 21 09:23:28 2018 Return-Path: {alex-at-hyipme.com} Received: from hyipme.com (vmi97812.contabo.host [80.241.218.115]) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w3LENSbB017077; Sat, 21 Apr 2018 09:23:28 -0500 DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=hyipme.com; s=mail; h=Message-Id:Content-Transfer-Encoding:Content-Type:MIME-Version:Date:Subject:From:Reply-To; bh=BSEOTWycQoIcv0Qn6DOK1Ltq2NthYcWRKqeCFNKaE48=; b=OR251h4xcJswo/K+dls+NDKldbzI+wxvEuTNzTCyZrguLijB5WWNJDD5zeSCIRvrR0ogz/UMd4f4x0e9EZgC2lJ6tKfZopZRdGrNEbdFpsGPy2UD6iX0TVHKdzi5hsSmbq6IYEd2aeDUvpX/D1WrQssakClRYxo2qoHaXN1Ge0Q=; Received: from ip97.ip-149-56-254.net ([149.56.254.97] helo=User) by hyipme.com with esmtpa (Exim 4.82) (envelope-from {alex-at-hyipme.com} ) id 1f9tPv-0002vL-Ix; Sat, 21 Apr 2018 16:23:07 +0200 Reply-To: {solution-finance-at-hotmail.com}
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Email: lopezcl-at-ohsu.edu Name: Claudia Lopez
Organization: OREGON HEALTH & SCIENCE UNIVERSITY
Title-Subject: [Filtered] Job opportunity at OHSU
Message: Dear microscopy community, The Multiscale Microscopy Core (MMC), located on Oregon Health and Science Universitys South Waterfront Campus is currently looking for an electron microscopy technician at the Research Assistant 2 level. The MMC (http://www.ohsu.edu/xd/research/research-cores/multi-scale-microscopy-core/index.cfm) is a state of the art electron microscopy university core that provides imaging, training and technical support to researchers in the Pacific Northwest. The Research Assistant 2 will provide support to the MMCs activities by preparing and processing samples and assisting the team in meeting its goals. For more information please visit OHSUs job website using the IRC code IRC68462. Kind regards,
Claudia
Claudia S. López, PhD Research Assistant Professor Department of Biomedical Engineering Director Multiscale Microscopy Core Phone: 503-418-0186
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The Advanced Imaging Center (AIC) at the Howard Hughes Medical Institute Janelia Research Campus and Janelia Group Leader Dr. Harald Hess are working together to introduce focused ion beam scanning electron microscopy (FIB-SEM) to the imaging center. We are actively looking for a research specialist/application scientist. The FIB-SEM we are building is custom-designed to be able to operate seamlessly for very long-term milling/imaging experiment (several months to a year is routine). The AIC FIB-SEM unit will be the 9th system of a large fleet of FIB-SEMs at Janelia.
More details on the FIB-SEM position can be found here: https://tinyurl.com/y998bx5p
The AIC is rapidly expanding its scope and its portfolio, we are looking for more highly motivated talents to join our exciting team! This position may eventually also be involved in several advanced correlative imaging techniques currently being developed at Janelia.
Regards, Leong
--- Teng-Leong Chew Director, Advanced Imaging Center HHMI Janelia Research Campus 19700 Helix Drive Ashburn, VA 20147
I am pleased to announce the 2018 annual current EM Techniques workshop - Bio Imaging analysis will be held on May 24 and 25, 2018, at University of Maryland Baltimore. The program includes presentations on 2-D and 3-D image analysis using both open source and commercial software.
Applications that will be discussed/demonstrated include photoshop, ImageJ, Slicer, IMOD, MIPAR, Amira-Avizo, Imaris, Arivis, Dragonfly/ORS, and ImagePro. We are very excited that we will have onsite many image analysis experts and software developers to demonstrate common workflows for image analysis of biological specimens.
Demo software is available for download. Please visit the web site below for more information.
--- Sincerely, Ru-ching Hsia rhsia-at-umaryland.edu Associate Professor and Director Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
The University of Southern California, in conjunction with Rigaku, would like to extend an invitation for you to join us at a seminar and workshop on X-ray microscopy. This USC-Rigaku event will take place on Thursday, May 24, 2018 in Harkness Auditorium on USC's beautiful campus.
In this seminar, you will learn the latest advances in X-ray microscopy from the top level innovators in the field. You can also participate in the workshop to learn the basics of X-ray microscopy and have hands-on lab experience.
Thank you all for the helpful tips. Here is the follow up.
1. All the power supplies in the electrical closet are on except A7 (24V h.t.) and A8 (24V fil.). When I pushed the HT on/off button, A8 came on and the red LED of A9-X2 was on. Seems like it indicates a problem with the HT oscillator. After about 1 minute, both went off and HT cannot be activated.
2. S34 is closed during operation. However, it does not disengage when I lifted the gun. This could be a problem but I don't think it is related to not being able to turn on the HT.
3. S33 is also closed during operation and it is open when the gun is lifted.
4. The 4 fuses behind the Right Hand Panel are intact. They are labelled Z201-4.
5. Upon restart, the filament type defaults to LaB6. This should not prevent activating the HT or the filament, even if a tungsten filament is installed.
6. Wehnelt protection circuit is not triggered. There are no red LED lighted up for any parts of A9, except for X2 mentioned above.
7. Restarting the TEM does not help in turning on the HT.
8. On our CM100, HT defaults to 40kV upon startup. I tried various values of kV but still couldn't turn on the HT.
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (http://depts.washington.edu/if/)
On Thu, Apr 19, 2018 at 10:21 PM, {wpchan-at-uw.edu} wrote: } } Can I please get your advice on a problem with the HT on a Philips CM100? } } When I press the HT On/Off button to turn on the high tension, I got no } response, i.e., I cannot turn on the high tension. The vacuum are good, } both HiV and UHV lights are on. I pulled the power supply (A7) and found } that the fuses are intact. The voltage going into A7 is 227V. I also } measured the resistance across the HT On/Off push button. It is 12 ohm when } pressed and 0 ohm released. } } Is there anything obvious that I have missed? Thanks!
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Email: holpc-at-firstenergycorp.com Name: Chris Holp
Organization: FirstEnergy BETA Labs
Title-Subject: [Filtered] Photo editing and annotation software results
Message: A few weeks ago I posted a question to everyone, asking about photo editing software with an emphasis on vector graphics for annotations. Because there was interest expressed in the responses, at this time I want to summarize the replies that I received:
Adobe Illustrator was recommended by one member, and Inkscape was recommended by one member.
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I have three questions about some features that I found in different biologic samples. All samples were prepared in similar way with karnovsky fixation, reduced osmium tetroxide, uranyl acetate and standard epon embedding, no post-staining was performed. All images are located on Google Drive (I am providing direct links).
1) Virus particles? I have found some round membrane bound structure of 70nm diameter in two independent samples (from different geographical places 1000 km apart): mouse hybridoma cell line (in endoplasmic reticulum) and mouse brain (around capillaries). These vesicles looks like some virus and this virus seems to have the same structure in both samples. Is a viral infection so common in mice and mouse cell lines? And what kind of virus could it be?
2) Mycoplasma or cell debris? In fibroblast cell culture there are some membrane bound structure of 0.5-1 um size that are located outside cells. Is it mycoplasma infection or some cell debris?
3) Precipitate or not? On several occasions I have seen very fine (5-6nm) and dense precipitate in different samples. No colloidal gold was used. This precipitate tends to concentrate in endosome-like structures and in cytoplasm. However, nucleus, mitochondria, ER lumen ususally do not contain this kind of precipitate. Is it just precipitate or these densely stained minuscule granules correlate with some biological structures? I have two unrelated samples that demonstrate similar phenomena: endothelial cells in mouse brain and macrophages in mouse lung. Not all cells contain this kind of staining and this as well make me think that it may stain some real biological structure.
We’re looking to purchase a new stereomicroscope for loading of TEM sample holders and I wanted to learn more about what models others are using. We thought it might be helpful to have a digital viewing screen, though we’re not opposed to using traditional binoculars.
Any suggestions? Thanks!
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
Please see below for details about an exciting upcoming meeting we are planning for October 8-10, 2018 at PNNL. More details about the agenda and abstract submission will be sent in a couple weeks. We hope that you will be able to attend!
----------------
Next-Generation Transmission Electron Microscopy (NexTEM) Workshop Beyond Current Limits of Resolution, Environments, and Data Analysis
Overview
Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.
Topics
High-Resolution Imaging and Spectroscopy – New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems. – Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision. – Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces. – Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples. – Novel detectors for improved high-speed imaging and spectroscopy.
In Situ / Operando Microscopy and Extreme Environments – Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids. – Investigation of materials under stimulus across a range of sample environments and temperatures.
Computational Methods for Data Analysis – Software to improve data collection quality, accuracy, and acquisition rates. – Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis. – Machine learning approaches for high-throughput data processing and feature detection. – Image simulation tools to aid the interpretation of experimental images and spectroscopy.
Confirmed Invited Speakers – Ondrej Krivanek, Nion Co. – Amanda Petford-Long, Argonne National Laboratory – Robert Klie, University of Illinois–Chicago – Khalid Hattar, Sandia National Laboratories – Renu Sharma, National Institute of Standards and Technology – Marc DeGraef, Carnegie Mellon University – James LeBeau, North Carolina State University – Haimei Zheng, Lawrence-Berkeley National Laboratory – Colin Ophus, Molecular Foundry – Juan Carlos Idrobo, Oak Ridge National Laboratory – R. Lee Penn, University of Minnesota – Paolo Longo, Gatan – Lewys Jones, Trinity College–Dublin – Rama Vasudevan, Oak Ridge National Laboratory
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
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Title-Subject: [Filtered] TEM: cooling water in Jeol JEM-3010
Message: Dear Listers, I'm using the on-line form for some problems with automatic attachment to my organization e-mails.
I'm looking for some advice about the cooling water flows of the JEOL JEM-3010 in our lab.
The cooling water flow of the the GATAN Model 794 CCD camera has fallen to 8 l/h, below the minimun of 9 l/h required in the manual. This water line is in parallel with the one cooling the diffusion pump of the microscope, and I can recover the right value for the CCD water flow by readjusting pressure (from 250 to 260 kPa) and flowrate (from 160 to 170 l/h) of the DP line . Now, the doubt is that the DP water flow is currently set at a value higher than the maximun one required, which I believe is about 120 l/h.
Otherwise, I should try to replace the cooling water line of the CCD camera - maybe it is just dirty - but I don't feel very comfortable in taking away the pipes directly from the body of the CCD camera: I'm afraid that its alignment could be lost.
Any advice will be greatly appreciated. Thanks in advance.
X-from: Alberto Diaspro {sekkio-at-mac.com} —— Dear friends,
Diaspro Lab - https://www.iit.it/index.php/people/alberto-diaspro - offers a great Nanoscale School at the Venetian Institute of of Sciences (IVSLA), Letters and Arts. All info at https://mix.iit.it/events/ivsla-2018#link_tabfor program, registration and updates and a limited number of seats.
IVSLA International School on Nanoscale Optical Microscopy Directors: Alberto Diaspro (IIT, UNIGE), Paolo Bianchini (IIT), Giorgio Giacometti (IVSLA).
12 - 15 June 2018, Venice, Italy
The objective of this School is to advance the field of nanoscale optical microscopy operating at the scale of nanometres to tens of nanometres, through the exchange of information, ideas, and innovative techniques. The understanding of methods and techniques has the great potential of allowing, in the near future, for the design and performance of new exciting experiments in Biophysics, Optics and Photonics. Shots of current technologies by companies leaders in the field.
An amazing blend of top level lecturers ready for lessons, seminars and student interactions in the amazing scenario offered by Venice in June.
Confirmed Faculty: • Francesca Romana Bertani • Paolo Bianchini • Gertrude Bunt • Loredana Casalis • Giberto Chirico • Nader Cristelle • Alessandro Esposito • Pekka Hänninen • Mike Heilemann • Luca Lanzanò • Aymeric Le Gratiet • Davide Mazza • Ammasi Periasamy • Alexander Rorbach • Colin Sheppard • Peter So • George Stanciu • Peter Saggau • Peter Török • Alessandro Tredicucci • Fred Wouters • Alberto Diaspro.
Lessons will be held in the magnificent Palazzo Loredan, Venice - https://youtu.be/fmuk-wKMuEI.
Due to high number of requests we added more seats. No deadline: first in first out acceptance criteria. Special fees for IIT/UNIGE, SIOF and SIBPA members.
See you in a fabulous Venice in June. All the best Alby
David-Couple of tricks here: first, check the filter in your chiller. If it is torn, you can get some crud passed to the microscope which then gets lodged in the camera before it causes a problem with the microscope. The first step to dislodge crud from the cooling lines is disconnect the upstream quick-disconnect connector, and reconnect it multiple times. The idea is to get air in the line so that bubbles form. Hopefully you have transparent lines and can watch the bubbles pass through. If that does not work, you will need to shut off the camera power, shut off the water supply, remove the quick-disconnect connectors and blow out the lines. After re-assembly, check the camera temperature using the software.
A. John Mardinly, Ph.D., P.E.
} On Apr 27, 2018, at 4:25 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=R8sHoV3q1b4fDgy9PDmFcAXp5axfLW8xEVYKz5O5g3A&s=Pgv7XdffZyT_T3-waAPovopV4J5ZW1xxDLB55OAXMyM&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=R8sHoV3q1b4fDgy9PDmFcAXp5axfLW8xEVYKz5O5g3A&s=ztlpVj5GZxYV5wk_TvCaJ_fY2ZbVula4XPwkE-3KSb0&e= } ---------------------------------------------------------------------------- } } } -------- Forwarded Message -------- } } } X-from: dcristofori-at-unive.it } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MLFormMail.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=R8sHoV3q1b4fDgy9PDmFcAXp5axfLW8xEVYKz5O5g3A&s=DNEsR7o3vs5gSBx6wdZjdaaM4u2dSXyBjuzcg9MJRYg&e= } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } dcristofori-at-unive.it as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: dcristofori-at-unive.it Name: Davide Cristofori } } Organization: Universita' Ca' Foscari Venezia } } Title-Subject: [Filtered] TEM: cooling water in Jeol JEM-3010 } } Message: Dear Listers, } I'm using the on-line form for some problems with automatic attachment to my organization e-mails. } } I'm looking for some advice about the cooling water flows of the JEOL JEM-3010 in our lab. } } The cooling water flow of the the GATAN Model 794 CCD camera has fallen to 8 l/h, below the minimun } of 9 l/h required in the manual. This water line is in parallel with the one cooling the diffusion } pump of the microscope, and I can recover the right value for the CCD water flow by readjusting } pressure (from 250 to 260 kPa) and flowrate (from 160 to 170 l/h) of the DP line . Now, the doubt } is that the DP water flow is currently set at a value higher than the maximun one required, which I } believe is about 120 l/h. } } Otherwise, I should try to replace the cooling water line of the CCD camera - maybe it is just dirty } - but I don't feel very comfortable in taking away the pipes directly from the body of the CCD } camera: I'm afraid that its alignment could be lost. } } Any advice will be greatly appreciated. } Thanks in advance. } } Davide } } } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } Davide Cristofori } } direct phone = 041.234.67.26 } e-mail = dcristofori[at]unive[dot]it } } Centre for Electron Microscopy "Giovanni Stevanato" and } Department of Molecular Sciences and Nanosystems } Ca' Foscari UNiversity of Venice } } Campus Scientifico, Edificio ETA } Via Torino 155 I-30172 Mestre (VE) Italy } ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~ } } } Login Host: 157.138.23.17 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 23, 53 -- From microscopy.listserver-at-gmail.com Fri Apr 27 18:06:59 2018 } 23, 53 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) } 23, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w3RN6xBt029008 } 23, 53 -- for {microscopy-at-microscopy.com} ; Fri, 27 Apr 2018 18:06:59 -0500 } 23, 53 -- Received: by mail-it0-f46.google.com with SMTP id 71-v6so3691702ith.2 } 23, 53 -- for {microscopy-at-microscopy.com} ; Fri, 27 Apr 2018 16:07:17 -0700 (PDT) } 23, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 23, 53 -- d=gmail.com; s=20161025; } 23, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 23, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 23, 53 -- bh=wXfYghoDqJWghZKY52RoWB3xW1jMl6WP2aiuSKVFLVM=; } 23, 53 -- b=VRYppfj0jKQwo0a14OVmj2OVe1LG7gk7AjIzR6SWO8ExMMCqmz/yuiTnQss9g6Ynxh } 23, 53 -- usJIUmm4UZt0MURQbHxwM0KuetW6aF94SMPLnGpnpDfJ06qQDZhzTGrdm0IEDX7lDr4r } 23, 53 -- Od9ZRPag/Z6IMjXNM+g+vap2LdwVi8toJWanGPZ32CNpz2I8MkkiUWC+kzREcaKIHXFG } 23, 53 -- 2Ba9+AnqSk5utFLvHN5q2GQMa8EFlA3Ok1vfoE1O7CPQbOzEavRGFzrfKJy0NPEw5Opy } 23, 53 -- DmCeYuqezecT5J2kz7m7uSujePNGpxlapa2xUUGFneUzihG9utmxlbbewCci9Ngm4QEV } 23, 53 -- TpIA== } 23, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 23, 53 -- d=1e100.net; s=20161025; } 23, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 23, 53 -- :user-agent:mime-version:in-reply-to:content-language } 23, 53 -- :content-transfer-encoding; } 23, 53 -- bh=wXfYghoDqJWghZKY52RoWB3xW1jMl6WP2aiuSKVFLVM=; } 23, 53 -- b=bD9q0cuyAGjSshanZ9JY3GVAF4iBG5xrINu0N6uXKoBZDP4nilq73HUbnslk/M41v9 } 23, 53 -- Iv4BTLkfG1p41d/fUu2B4S3O1spwfH5VG5+tjrdz4xFnJ2vcwFbqa3mtOxhxHHKdz8ar } 23, 53 -- sFkzCZtLyUBCOv9IzCtRHg9VLNZfD8vd2XOFekDkT1N0kDnZa9Dg5kqqjMiu45ph9UF7 } 23, 53 -- TUlsD8GWFg4oZU9ywkA1F0g9Unci41EOoNY3jEua4w3fr+c4qJQ4aS5QgNx2j3puWLTa } 23, 53 -- xk0odq78rrQw6lWsv70/CSi1H5YaGuQ09JkGKYyr+JktwhkQOeZDu3tygM/IfzAxo1C7 } 23, 53 -- t8pg== } 23, 53 -- X-Gm-Message-State: ALQs6tBr0DFox1qYAfsqVrAAdIIB4hz6Elt5jXLJBrOKUDWscv3mnzkg } 23, 53 -- F0xiEDJgcaa90t5TuaAtKo4/Kpcc } 23, 53 -- X-Google-Smtp-Source: AB8JxZrqI+y7dyOo1WA/7At6KdrQ577AagOGhsiDu1Ucc/ClzHTYHJ4nZBQxLZ4IgkgJVC1pyUexlA== } 23, 53 -- X-Received: by 2002:a24:285:: with SMTP id 127-v6mr3907813itu.43.1524870436674; } 23, 53 -- Fri, 27 Apr 2018 16:07:16 -0700 (PDT) } 23, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:e1b6:e97a:1c06:6c8c]) } 23, 53 -- by smtp.googlemail.com with ESMTPSA id r1-v6sm1074362ioc.57.2018.04.27.16.07.15 } 23, 53 -- for {microscopy-at-microscopy.com} } 23, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 23, 53 -- Fri, 27 Apr 2018 16:07:15 -0700 (PDT) } 23, 53 -- Subject: viaWWW:3010 } 23, 53 -- References: {201804271621.w3RGLE9O003694-at-microscopy.com} } 23, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 23, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 23, 53 -- X-Forwarded-Message-Id: {201804271621.w3RGLE9O003694-at-microscopy.com} } 23, 53 -- Message-ID: {799bf11d-76ac-3a45-c56d-3e115de7c989-at-gmail.com} } 23, 53 -- Date: Fri, 27 Apr 2018 18:07:14 -0500 } 23, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 23, 53 -- Gecko/20100101 Thunderbird/52.7.0 } 23, 53 -- MIME-Version: 1.0 } 23, 53 -- In-Reply-To: {201804271621.w3RGLE9O003694-at-microscopy.com} } 23, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 23, 53 -- Content-Language: en-US } 23, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From koepketemi17ce-at-gmail.com Sun Apr 29 09:41:33 2018 Return-Path: {koepketemi17ce-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.160.248] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id w3TEfW1q008630 for {microscopylistserverarchive6-at-microscopy.com} ; Sun, 29 Apr 2018 09:41:33 -0500 Received: from unknown (86.73.60.168) by m1.gns.snv.thisdomainl.com with SMTP; Sun, 29 Apr 2018 06:37:55 -0800 Received: from webmail.halftomorrow.com ([80.52.77.139]) by relay.2yahoo.com with QMQP; Sun, 29 Apr 2018 06:33:29 -0800 Received: from [170.92.74.83] by rly04.hottestmile.com with QMQP; Sun, 29 Apr 2018 06:19:49 -0800 Received: from mtu67.syds.piswix.net ([30.5.37.211]) by nntp.pinxodet.net with SMTP; Sun, 29 Apr 2018 06:00:16 -0800 Received: from smtp-server1.cfdenselr.com [43.168.35.173] by nntp.pinxodet.net with NNFMP; Sun, 29 Apr 2018 05:57:44 -0800 Message-ID: {08DB39C4.7F370432-at-gmail.com}
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Message: EMS Microscopy Academy announces our Cryosectioning/Immunogold Workshop. Five days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in cryosectioning and immunogold labeling.
Led by experts in their fields, Helmut Gnaegi (DiATOME) and Peter van de Plas (Aurion), this class promises to teach you the most up-to-date techniques available.
June 4 - 8, 2018 Monday - Friday 9:00am - 4:30pm Hatfield, Pennsylvania, USA
Do not miss out! Visit the link below for more information: http://ow.ly/KhBB30i73CS
Also available: Three-week summer courses, where you can increase your knowledge and skills in the areas you prefer! www.emsdiasum.com/academy
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Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: University of Washington
Title-Subject: [Filtered] Razor blades for block trimming
Message: It seems as though the Solingen carbon steel razor blades are not available anywhere in the USA anymore. Does anyone have a secret source? I found them on a site in Germany but the cost is twice as much (and that's not including shipping!) that I've paid in the past. I've tried half a dozen and none come even close to being as good and/or long lasting for trimming blocks for microtoming.
Thoughts, experiences with other blades that are just as good? Cheers, Ellen Lavoie UW Molecular Analysis Facility
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Title-Subject: [Filtered] Philadelphia Society for Microscopy Spring Symposium
Message: Philadelphia Society for Microscopy SPRING SYMPOSIUM MAY 16 8:00 AM - 4:00 PM at the SINGH CENTER, UNIVERSITY OF PENNSYLVANIA
RESEARCH AT THE LEADING EDGE OF ELECTRON MICROSCOPY
SPEAKERS:
David C. Martin, University of Delaware In-Situ Imaging of Polymer and Organic Molecular Materials by Transmission Electron Microscopy
Bojeong Kim, Temple University Application of Analytical Transmission Electron Microscopy Techniques for the Discovery and Characterization of Nano-sized Environmental Samples
Masashi Watanabe, Lehigh University: MAS Tour Speaker, Keynote: Atomic-level Imaging and Analysis of Materials by Aberration-Corrected Scanning Transmission Electron Microscopy
Vera Moiseenkova-Bell, University of Pennsylvania Cryo Electron Microscopy at the Forefront of Molecular Medicine and Drug Discovery
Eric A. Stach, University of Pennsylvania Advanced Electron Microscopy of Materials at the Singh Nanotechnology Center
VENDOR EXHIBITS INCLUDING: Electron Microscopy Sciences, ThermoFisher Scientific (FEI), Structure Probe Inc., Gatan, I.Miller, IXRF and ThermoNoran AND -- A Tour of the Singh Nanotechnology Center
Registration, Schedule and Details: http://phillyscope.com/
Online Registration: $25-Regular Member / $10-Student FREE for Students who bring a poster to present. LUNCH INCLUDED with online Registration!
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What are the ingredients of Silly Putty FAQ | crayola.com {http://www.crayola.com/faq/another-topic/what-are-the-ingredients-of-silly-putty/} www.crayola.com Silly Putty is made primarily from silicone and color pigments. Silly Putty was discovered in 1943 by James Wright and introduced to the public in 1950 by Peter Hodgson. Crayola acquired the exclusive manufacturing rights to Silly Putty in 1977. The formulas are considered proprietary ...
or from WalMart
Duck Mounting Putty, Removable, 56G, 12PK/CT, BE - DUCPTY2CT Mounting Putty is ideal for temporary mounting of paper items such as posters, charts and decorations to nearly any surface without damage to paper item or surface you are mounting it on. It provides a safe alternative to tape, nails, glues, tacks and staples. Mounting putty is nontoxic and easily removable and reusable. Conforms to ASTM-4236.
best
cheers
ed
Edward J Basgall, PhD
Microscopy Technician
Physical address: Materials Characterization Core
106 Bossone Research Enterprise Center
3120 Market St., Philadelphia PA
Mailing address: Drexel University
3141 Chestnut St
MC 27-344
Philadelphia, PA 19104
Office: (215) 895-2379 FAX (215) 895- 6760
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Friday, April 27, 2018 7:30:57 PM *To:* Basgall,Edward *Subject:* [Microscopy] Fwd: Non Crystalline Clay or Putty
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X-from: rfklie-at-uic.edu
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Email: rfklie-at-uic.edu Name: Robert Klie
Organization: University of Illinois
Title-Subject: [Filtered] Post-doc Position in In-Situ TEM/STEM available at the University of Illinois - Chicago
Message: The University of Illinois at Chicago (UIC) invites applications to fill a Postdoctoral Research Associate position in the Department of Physics under the supervision of Professor Robert F. Klie. This position will be part of the Joint Center for Energy Storage Research (JCESR) at Argonne National Laboratory and focus on in-situ characterization of rechargeable battery cathode materials using high-resolution scanning transmission electron microscopy and spectroscopy. The goal of this research program is to correlate electro-chemical measurements with structural changes of the cathodes at the nano-scale. Close collaboration with JCESR scientists and access to the ANL facilities will be possible as part of this project.
Please use the following link to apply for this position: https://jobs.uic.edu/job-board/job-details?jobID=95602
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==============================Original Headers============================== 16, 54 -- From microscopy.listserver-at-gmail.com Thu May 3 19:34:40 2018 16, 54 -- Received: from mail-it0-f47.google.com (mail-it0-f47.google.com [209.85.214.47]) 16, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w440Yesi025303 16, 54 -- for {microscopy-at-microscopy.com} ; Thu, 3 May 2018 19:34:40 -0500 16, 54 -- Received: by mail-it0-f47.google.com with SMTP id n202-v6so1464042ita.1 16, 54 -- for {microscopy-at-microscopy.com} ; Thu, 03 May 2018 17:35:18 -0700 (PDT) 16, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=gmail.com; s=20161025; 16, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 16, 54 -- :in-reply-to:content-language:content-transfer-encoding; 16, 54 -- bh=vJnJcAqL0+C14iZ2Q8xq0LpYu47wFc9MEV8j8lrlRm0=; 16, 54 -- b=tyP25cPivxoWEZdFdWFWPQwPQdaA0E9WkjWFXQdmIwAQKIbPS06qA7p6u/8J/kNfWI 16, 54 -- 7RAdImVBW+Y3JtGtygK5L4DYDBYj6y1AAKtg94iE2ui2sWjEdZHKiZ+OBjO2txqBP7bQ 16, 54 -- CJXqsD/1/45i1NfFHSY0OLm42L5hVBqCBQgw5dslyfv2D7ewbADSwEuEcKczsDRvN7e7 16, 54 -- NiId5EUdq0nhlK1MLBvRzEi6+ZF5YB9hfrKyNqBBWvDU/7w/+b1O4mg1f1fJlWtagtsf 16, 54 -- dKSZky5RCDWjUD/T9xyduc9SV5G8dEeqJAYdYH/E785ZliObYGf/aKeoqKMssxqGHKci 16, 54 -- GsNQ== 16, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=1e100.net; s=20161025; 16, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 16, 54 -- :user-agent:mime-version:in-reply-to:content-language 16, 54 -- :content-transfer-encoding; 16, 54 -- bh=vJnJcAqL0+C14iZ2Q8xq0LpYu47wFc9MEV8j8lrlRm0=; 16, 54 -- b=FZZdglkeR2SPpBePpU9XSi4XZ3dUmU7R702WQPDjkQYBnCKvbDiLwheCqGhuy7l172 16, 54 -- irE3mRv+Df3+d6K80R5ce+oLy2D75ViswEChOT/XH+WEgzGM5HH6/ygkXhuaidJiliBi 16, 54 -- 6tW2GR7TvrWtDVRHyYA4kJOCJgpRtr0lUXO74HqZA8FARNOARAE+GfYj7lz9XquOzDOT 16, 54 -- FuXYa1fSJk0MTlEXaoWTl0RDcKOWMxm0CEbXvREv+FjDQxo/wBrDZQ/imXK42QryXzwd 16, 54 -- 4QlvABliHuqc/8rkVObOqHYWo+opv8xSem9NaXlRgNVbvi0YOn5eVAVfcg4UteKlzueP 16, 54 -- bzhA== 16, 54 -- X-Gm-Message-State: ALQs6tDlSVys+hiB/K0Yb1m7teEO5ghwibI93FuiN9UX9dsE1MVEQsKy 16, 54 -- NgumvwdfSr6Vr3qGo9hoIDhQrQzP 16, 54 -- X-Google-Smtp-Source: AB8JxZrjg4nIrcZ4WW7h5/SDy2aFfUA2z04htDoToQxGUmEKTr06KHX2QMvbjv9ZxKph81yBPNMSjw== 16, 54 -- X-Received: by 2002:a24:7f0d:: with SMTP id r13-v6mr27405832itc.39.1525394117530; 16, 54 -- Thu, 03 May 2018 17:35:17 -0700 (PDT) 16, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:6078:64a8:810f:2200]) 16, 54 -- by smtp.googlemail.com with ESMTPSA id c194-v6sm411305itb.25.2018.05.03.17.35.16 16, 54 -- for {microscopy-at-microscopy.com} 16, 54 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 54 -- Thu, 03 May 2018 17:35:17 -0700 (PDT) 16, 54 -- Subject: viaWWW:Post-doc Position in In-Situ TEM/STEM available at the 16, 54 -- University of Illinois - Chicago 16, 54 -- References: {201805021643.w42GhNMr013094-at-microscopy.com} 16, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 16, 54 -- X-Forwarded-Message-Id: {201805021643.w42GhNMr013094-at-microscopy.com} 16, 54 -- Message-ID: {0772b4cc-5acf-2918-5e0e-f854b96912c9-at-gmail.com} 16, 54 -- Date: Thu, 3 May 2018 19:35:16 -0500 16, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 16, 54 -- Gecko/20100101 Thunderbird/52.7.0 16, 54 -- MIME-Version: 1.0 16, 54 -- In-Reply-To: {201805021643.w42GhNMr013094-at-microscopy.com} 16, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 16, 54 -- Content-Language: en-US 16, 54 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] Engineer XRD - SEM Position at the Research Service Centers
Message: Classification Title: Engineer II Job Description: -Operation of the instrumentation in the facility to include: 1) X-ray diffraction instrumentation 2) Scanning Electron Microscopy 3) Energy Dispersive Spectroscopy 4) Raman Spectroscopy 5) Fourier Transformed Infrared analysis as indicated below: -Operate the equipment for service to faculty, students and external users -Train faculty and students on the use of the equipment and instruments software for data reduction and analysis -Assist with the laboratory practices for formal and short courses on X-ray diffraction -Develop operating procedures and use protocols for the facilities under his/her supervision. -Maintain the equipment, install new instrumentation and software, monitor computer records of equipment use, and supervise scheduling of the equipment use. -Other job duties as assigned to support the RSC. Advertised Salary: $50,000 - $55,000 commensurate with qualifications and experience Minimum Requirements: Bachelor's degree in an appropriate area and two years of relevant experience; or a high school diploma or equivalent and six years of relevant experience. Appropriate college coursework or vocational/technical training may substitute at an equivalent rate for the required experience. Preferred Qualifications: Masters degree in appropriate area of specialization Prefer advanced degree in physics, chemistry, materials science, electrical engineering or chemical engineering. Knowledge of materials characterization and analysis techniques Experience with vacuum technology and gas handling systems Experience with software for data reduction and analysis Experience with X-ray diffraction instrumentation Experience with Scanning Electron Microscopy Experience with Energy Dispersive Spectroscopy Experience with Raman Spectroscopy Experience with Fourier Transformed Infrared analysis Ability to work independently Knowledge of laboratory safety practices Ability to establish and maintain effective working relationships with others Ability to communicate clearly both verbally and in writing Ability to maintain records and create reports Ability to interact cordially with coworkers to accomplish common tasks.
Special Instructions to Applicants: Please apply at: http://explore.jobs.ufl.edu/cw/en-us/job/506687/engineer-ii
In order to be considered, applicants must upload a cover letter and resume. Application must be submitted by 11:55 p.m. (ET) of the posting end date. This requisition has been reposted. Previous applicants do not need to reapply.
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==============================Original Headers============================== 16, 53 -- From microscopy.listserver-at-gmail.com Thu May 3 19:35:30 2018 16, 53 -- Received: from mail-it0-f46.google.com (mail-it0-f46.google.com [209.85.214.46]) 16, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w440ZU62025977 16, 53 -- for {microscopy-at-microscopy.com} ; Thu, 3 May 2018 19:35:30 -0500 16, 53 -- Received: by mail-it0-f46.google.com with SMTP id e20-v6so1520382itc.1 16, 53 -- for {microscopy-at-microscopy.com} ; Thu, 03 May 2018 17:36:07 -0700 (PDT) 16, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 53 -- d=gmail.com; s=20161025; 16, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 16, 53 -- :in-reply-to:content-language:content-transfer-encoding; 16, 53 -- bh=35UneLTrz/iMiL0nD/E9MvJV6GZ1e4Le6V8imifso7o=; 16, 53 -- b=eGqb0LcH3VqOK5nFqtWA7CIIRycOyBYNTMaGQn3m0PB0r0xttxLWfHMLddaFCxon7w 16, 53 -- ft556ku6UEtMYPxihBEcLJVcqMkR1tbhH+PV/xU+wz9XvLfgx4MGNZhiBLQ4fphRNIU0 16, 53 -- OL93/22MsOo6+tcISR86jMUIPbBbaVdSHvLjfNGsBo2rIpFVIMvkVKHtagB6xmCBk3lL 16, 53 -- F50b/K4bOU6nzQJ/nYioSG/INUZgdVi/IXJHy5a+nBU3Td6yziTzWfs/cfQGqC+/usYu 16, 53 -- hOHIQNub9L3KR5QNNlrkFQoxeoMkbNX3eoQ2jZThxLL1YbTMX8oWs9M7J9MGohqbXnm5 16, 53 -- yhrg== 16, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 53 -- d=1e100.net; s=20161025; 16, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 16, 53 -- :user-agent:mime-version:in-reply-to:content-language 16, 53 -- :content-transfer-encoding; 16, 53 -- bh=35UneLTrz/iMiL0nD/E9MvJV6GZ1e4Le6V8imifso7o=; 16, 53 -- b=iLT+H+Z+WABeH1vnRktbrhg/fgayGBYu4t57BzuyHbwdX7e4qNzajPZxc8+wOxgp9I 16, 53 -- 2Cp65gBSqbjXe2qA9OHWnkXCiUayxs7T09RKjgXBp+KvTWbfQkprQU2bYrPC20c20OhD 16, 53 -- uqPgMhbOW/zlhjvuTIQQ3StvmiCoxUgHE8J9QgGit08zBj7kX9b5zVj2brhEcjhh1cE3 16, 53 -- s/eYOgwdCPcX2YKeFSBnb0XKy6h00oMB+j9HR4/xzRsctmXtRYyeajOcxpUg8bwA4YMj 16, 53 -- oiW/kc/VOkPlIzDjSwuv3uZJ3cyNlScHm5VnuWVcnO2lghb9OvZNp0fOiuVNMUVzXlyb 16, 53 -- x8Hg== 16, 53 -- X-Gm-Message-State: ALQs6tC+1o2PUW8KWQpEyMYCdqLdHULexTVa4L2p5jEfEs+ZkYGqkpSL 16, 53 -- qF73J3xDAR8MR7Fs9hjZTHIu5dRo 16, 53 -- X-Google-Smtp-Source: AB8JxZpcqlHMzPaCw2eSeaAi0SEZzJCF/VFJnXjiZndDmG44NNa/nVj0P6HQJEaA4Kwp+437o3zueQ== 16, 53 -- X-Received: by 2002:a24:da02:: with SMTP id z2-v6mr8274375itg.145.1525394167039; 16, 53 -- Thu, 03 May 2018 17:36:07 -0700 (PDT) 16, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:6d5d:8b93:80f0:a91b]) 16, 53 -- by smtp.googlemail.com with ESMTPSA id u2-v6sm3563594ioc.8.2018.05.03.17.36.06 16, 53 -- for {microscopy-at-microscopy.com} 16, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); 16, 53 -- Thu, 03 May 2018 17:36:06 -0700 (PDT) 16, 53 -- Subject: viaWWW: Engineer XRD - SEM Position at the Research Service Centers 16, 53 -- References: {201805021948.w42Jmqup028755-at-microscopy.com} 16, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 16, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 16, 53 -- X-Forwarded-Message-Id: {201805021948.w42Jmqup028755-at-microscopy.com} 16, 53 -- Message-ID: {d3e7e52c-bb09-572d-1101-74f57cb3e985-at-gmail.com} 16, 53 -- Date: Thu, 3 May 2018 19:36:05 -0500 16, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 16, 53 -- Gecko/20100101 Thunderbird/52.7.0 16, 53 -- MIME-Version: 1.0 16, 53 -- In-Reply-To: {201805021948.w42Jmqup028755-at-microscopy.com} 16, 53 -- Content-Type: text/plain; charset=UTF-8; format=flowed 16, 53 -- Content-Language: en-US 16, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Dear All, I would like to ask for your advice, if it is 'good to have' or a 'must' to equip a light microscope with the differential interference (DIC) contrast kit to view the thin sections from ultramicrotomy? What are the benefits/information can we gain when we use the DIC to look at the thin sections before we use the TEM to view them?
Lastly, do we need both reflectance and transmittance DIC to view the thin sections or either one of them? I ask this because we are running a tight budget to get a new light microscope.
IST Austria is a constantly growing international institute for conducting frontier research in the life, physical, and formal sciences, located in Klosterneuburg on the outskirts of Vienna in Austria.
Electron Microscopy facility at IST Austria is expanding in the cryo-EM field. The complete cryo-EM infrastructure, which covers both SPA and cryo-ET workflow on the top level, is going to be established at IST Austria within this year. Following setups are going to be installed: 300kV TEM Titan Krios (Phase-plate, 2x DDD, BioQuantum); 200kV TEM Glacios (Phase-plate, DDD), Cryo-FIB/SEM Aquilos. To strengthen our team, we are looking for a person fulfilling our requirements who will be highly motivated to take over cryo-EM related responsibility in a challenging international environment.
*Responsibilities*
* Operating and maintenance of cryo-EM equipment. * Monitoring of systems, regular system check including systems alignment. * Training, assistance, on-site support and supervision of users. * Monitoring of compliance with rules. * Active contribution to the development of the EM facility.
*Requirements*
* An advanced degree (M.S./Ph.D.) in Biological Sciences, Biochemistry, Structural Biology or Physics. * Practical experience with cryo-EM application areas (sample preparation, single particle and/or tomography data collection, data processing), which includes all or most of the following SW: EPU, FEI Tomography suite, Serial EM, Imod, Relion. * Experience with cryo-CLEM and FIB milling is an advantage. * Min. 2 years of high-end cryo-EM hands-on experience. * Excellent team player that can also work independently. * High degree of reliability, organizational and interpersonal skills. * Service oriented approach towards researches. * Excellent communication, presentation and writing skills in English.
*To apply for this position send your application in one combined pdf (including CV, certificates and references) by e-mail to:* recruiting-at-ist.ac.at {mailto:recruiting-at-ist.ac.at}
X-from: Stuart Kearns {Stuart.Kearns-at-bristol.ac.uk}
Hi All,
EMAS (European Microbeam Analysis Society) and the Mineralogical Society of GB and Ireland are running a workshop aimed at post-graduate students and research workers on Microbeam techniques in the Earth Sciences.
The training workshop will run from 4th – 7th September at the University of Bristol, UK
Topics covered include SEM, EPMA, CL, EBSD, TEM, LA-ICPMS, SIMS, XANES, XRF, FIB, Raman, FTIR, MicroCT and APT, with presentations consisting of tutorial on the technique by analytical experts followed by application talks from renowned Earth Scientists. Also poster sessions and contributed talks.
Student early registration is £350 including accommodation (and most meals) and there are bursaries available to help with these costs.
Deadline for abstracts for bursary applications is 15th May. https://www.microbeamanalysis.eu/events/event/51-emas-2018-microbeam-analysis-in-the-earth-sciences
If anybody would like a poster for the event, please e-mail me offline and I will forward a pdf.
Best wishes, Stuart ------------------------------------------------- Stuart Kearns School of Earth Sciences University of Bristol, UK Tel. +44 (0)117 331 5004/5012 Stuart.Kearns-at-bristol.ac.uk -------------------------------------------------
In TV-Scan I get these black and white bars, in Slowscan1 I get gibberish which might come from the alphanumeric characters showing up at the top of the TV-scan frame.
I do so for a short time the "normal" scan image with WD and HV values displayed at the bottom of the frame when I press the CPU reset switch at the console.
Since the some parts of the SEM vanished during a two year storage (like keyboard and service tools) it also could be the case that the SEM needs the attached keyboard to properly display the scans.
Vacuum and HV is working but I don`t see any change when heating up the cathode and checking for a video signal.
I took out all the PCBs and cleaned the connectors. No change...
Does anybody have an idea what might be the fault? Could an empty backup battery at the FIS board be one part of the problem?
I am also loooking for a spare keyboard. Can I use one of the JSM 840 keyboards also?
And I am looking for a set of service tools...
Thanks for helping,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Title-Subject: [Filtered] PhD and Postdoctoral job openings at University of Antwerp - Belgium (EMAT)
Message: Dear all,
The electron microscopy group at the University of Antwerp (EMAT) is currently advertising a series of PhD and postdoctoral positions on state-of-the-art developments in transmission electron microscopy. Information about these positions can be found here:
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Email: david_rollings-at-tedpella.com Name: David Rollings
Organization: Ted Pella Inc
Title-Subject: [Filtered] Materials Science Product Specialist position open
Message: We are seeking a Materials Science Product Specialist to join our dynamic team in support of our sample preparation product lines. This is a great opportunity involving product development and growth. Candidates must have a BS in Materials Science or related field, with a minimum of 5 years hands-on SEM and 2 years hands TEM with hands-on specimen prep for both, beyond an academic environment. EDX and WDS experience desired, AFM experience a plus. Experience in the semiconductor industry is preferred, experience in the forensic science field is a plus. Contact human_resources-at-tedpella.com for complete job posting or to submit a resume. More information at www.tedpella.com Login Host: 12.7.209.242 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: mholman-at-mvainc.com Name: Melissa Holman
Organization: MVA Scientific Consultants
Title-Subject: [Filtered] Deben BSD on JEOL SEM
Message: Does anyone have experience with installing a Deben BSD on a JEOL SEM (any JEOL SEM)? Looking for some feedback as we consider making a purchase.
Thanks! Melissa Holman MVA Scientific Consultants
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Hi all, We have a JEOL 2100F with a high-tilt pole piece installed (and no aberration corrector), and we've noticed that the aberrations picked up in the objective lens strongly depend on beam shift. Even with properly aligned shift and tilt compensation, and with mini-condenser and objective mini-lens turned off, a beam shift of ~20 um in either direction turns an initially round, focused, well stigmated beam to acquire a massive coma. This seems to happen in the objective lens. Equivalently, expanding the beam past a certain point with the condenser system produces huge caustics at the edges. We've noticed that this doesn't happen when we defocus the objective lens by at least 100 um in either direction from the 'standard focus' that we set according to the service manual, but it's then a little harder to put the specimen in an image plane.
Has anybody else experienced this with this pole piece or a different one on the 2100F or a similar instrument? If so, how did you fix it? Did you just set a new 'standard focus' and align the projection/imaging system to compensate for the defocus? Tyler
-- Dr. Tyler Harvey Georg-August-Universität Göttingen IV. Physical Institute Friedrich-Hund-Platz 1 37077 Göttingen Germany
==============================Original Headers============================== 4, 33 -- From trh-at-uoregon.edu Wed May 9 04:03:40 2018 4, 33 -- Received: from smtp.uoregon.edu (cc-smtp4.uoregon.edu [184.171.108.232]) 4, 33 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w4993enX016596 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 9 May 2018 04:03:40 -0500 4, 33 -- Received: from mail-lf0-f48.google.com (mail-lf0-f48.google.com [209.85.215.48]) 4, 33 -- (authenticated bits=0) 4, 33 -- by smtp.uoregon.edu (8.14.4/8.14.4) with ESMTP id w4994WTk004322 4, 33 -- (version=TLSv1/SSLv3 cipher=AES128-GCM-SHA256 bits=128 verify=NOT) 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 9 May 2018 02:04:34 -0700 4, 33 -- Received: by mail-lf0-f48.google.com with SMTP id g12-v6so49864925lfb.10 4, 33 -- for {microscopy-at-microscopy.com} ; Wed, 09 May 2018 02:04:33 -0700 (PDT) 4, 33 -- X-Gm-Message-State: ALQs6tApirSMt3WUl1IG9ouY0IJkyucQ2sLQpeNQ1XlxHBeqD6iRoQEF 4, 33 -- XHNpQj9y/vUPBjuZsjkkTqkNmc2dmI3OD9E6DU7pOA== 4, 33 -- X-Google-Smtp-Source: AB8JxZqtQcigcefgZNsd1jbq3X60uH/HxZ60T8wAfnr+dsFEAe9MvkjdwQD30R7MSNnUnmBKD3NMC2b12LKpUguLp90= 4, 33 -- X-Received: by 2002:a19:d763:: with SMTP id o96-v6mr27245809lfg.89.1525856672287; 4, 33 -- Wed, 09 May 2018 02:04:32 -0700 (PDT) 4, 33 -- MIME-Version: 1.0 4, 33 -- Received: by 10.46.113.25 with HTTP; Wed, 9 May 2018 02:04:11 -0700 (PDT) 4, 33 -- From: Tyler Harvey {trh-at-uoregon.edu} 4, 33 -- Date: Wed, 9 May 2018 11:04:11 +0200 4, 33 -- X-Gmail-Original-Message-ID: {CAPgw9wohrhPBs5EYy6_9BNA059zS2ALt4M4aaCbNyFrAVCa+YA-at-mail.gmail.com} 4, 33 -- Message-ID: {CAPgw9wohrhPBs5EYy6_9BNA059zS2ALt4M4aaCbNyFrAVCa+YA-at-mail.gmail.com} 4, 33 -- Subject: aberrations with JEOL 2100F high-tilt pole piece 4, 33 -- To: microscopy-at-microscopy.com 4, 33 -- Content-Type: text/plain; charset="UTF-8" 4, 33 -- X-Proofpoint-Virus-Version: vendor=fsecure engine=2.50.10434:,, definitions=2018-05-09_04:,, 4, 33 -- signatures=0 4, 33 -- X-Proofpoint-Spam-Details: rule=notspam policy=default score=0 suspectscore=3 malwarescore=0 4, 33 -- phishscore=0 bulkscore=0 spamscore=0 mlxscore=0 mlxlogscore=643 4, 33 -- adultscore=0 classifier=spam adjust=-40 reason=mlx scancount=1 4, 33 -- engine=8.0.1-1711220000 definitions=main-1805090088 4, 33 -- Content-Transfer-Encoding: 8bit 4, 33 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w4993enX016596 ==============================End of - Headers==============================
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Title-Subject: [Filtered] SEM rotary pump's gas ballast?
Message: Hello.
I was replacing mist filters for our JEOL SEM's rotary pumps recently, when it occurred to me that I never ballasted the pumps. In my previous job, I used a lot of mass spectrometers, and we ballasted the rotary pumps once a week. Mass spectrometers handle a lot of solvents, while SEMs don't. So I'm guessing that we almost never have to ballast rotary pumps for SEM. Am I correct? Our SEM has Variable Pressure capability, but we don't analyze wet or moist samples frequently. If anybody has any thought on this subject, I would appreciate it.
Thank you.
---------------------------------------------------------- Tsutomu "Shimo" Shimotori Research Instrumentation Laboratory manager University of Minnesota - Duluth
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Mist filters? Are you dumping pump exhaust into interior air that people actually breath? Is this legal anywhere anymore? It’s certainly not a ‘best practice’.
A. John Mardinly, Ph.D., P.E.
} On May 9, 2018, at 5:33 AM, microscopy.listserver-at-gmail.com wrote: } } } } } } X-from: shim0102-at-umn.edu } } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy both } shim0102-at-umn.edu as well as the Microscopy Listserver } --------------------------------------------------------------------------- } } Email: shim0102-at-umn.edu Name: Tsutomu "Shimo" Shimotori } } Organization: University of Minnesota Duluth } } Title-Subject: [Filtered] SEM rotary pump's gas ballast? } } Message: Hello. } } I was replacing mist filters for our JEOL SEM's rotary pumps recently, when } it occurred to me that I never ballasted the pumps. } In my previous job, I used a lot of mass spectrometers, and we ballasted } the rotary pumps once a week. } Mass spectrometers handle a lot of solvents, while SEMs don't. } So I'm guessing that we almost never have to ballast rotary pumps for SEM. } Am I correct? } Our SEM has Variable Pressure capability, but we don't analyze wet or moist } samples frequently. } If anybody has any thought on this subject, I would appreciate it. } } Thank you. } } ---------------------------------------------------------- } Tsutomu "Shimo" Shimotori } Research Instrumentation Laboratory manager } University of Minnesota - Duluth } } Login Host: 131.212.168.48 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
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Wed, 9 May 2018 6, 96 -- 20:43:39 +0000 6, 96 -- From: John Mardinly {John.Mardinly-at-asu.edu} 6, 96 -- To: MSA {Microscopy-at-Microscopy.com} , "shim0102-at-umn.edu" {shim0102-at-umn.edu} 6, 96 -- Subject: Re: [Microscopy] viaWWW: SEM rotary pump's gas ballast? 6, 96 -- Thread-Topic: [Microscopy] viaWWW: SEM rotary pump's gas ballast? 6, 96 -- X-ASG-Orig-Subj: Re: [Microscopy] viaWWW: SEM rotary pump's gas ballast? 6, 96 -- Thread-Index: AQHT55IdtxHqaa9MkkaBsozpZDpUDqQn3akA 6, 96 -- Date: Wed, 9 May 2018 20:43:39 +0000 6, 96 -- Message-ID: {3B422D8B-7DA0-4FB9-9546-D4766CF4BCC2-at-asu.edu} 6, 96 -- References: {201805091233.w49CXgQL029383-at-microscopy.com} 6, 96 -- In-Reply-To: {201805091233.w49CXgQL029383-at-microscopy.com} 6, 96 -- Accept-Language: en-US 6, 96 -- Content-Language: en-US 6, 96 -- X-MS-Has-Attach: 6, 96 -- X-MS-TNEF-Correlator: 6, 96 -- x-originating-ip: [2600:8800:1e86:a7fb:3c5a:ca26:3f3d:98b8] 6, 96 -- x-ms-publictraffictype: Email 6, 96 -- x-microsoft-exchange-diagnostics: 1;BLUPR0601MB1491;7:couRZOnsrJREyZqHYcPVTnIIJ1Axh9MsSkQ9KYxM6eIrfn2sMhpNa9XCoValmqkdFVm6D4BGBeOvlmzIPdn72qwBma1fjOVZS1Cw3XSFr8FWN1Y1QD7uwFvHriP33RYq/mHhK+NDYXx+F+QZZ4O0z9EyEa9J3toyUVZECBpgJbWXJLLiYAKO72MnP6QUZ/YOAT+2RrNMS7JnsggthOrxKNv9x0YwtMAa8Um9JkB6kQ869eFatbSZLBxEGunhVDfx 6, 96 -- x-ms-exchange-antispam-srfa-diagnostics: SOS; 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We always ballasted TEM rotary pumps once a week, back in the day when they used film. Wouldn't seem to be necessary with an SEM, unless you use LV mode a lot with dirty specimens. I'm not sure that it would hurt, but in changing RP oil in our SEM once a year, I don't see any problems with the used oil coming out of the pump. We use LV mode occasionally as well, but common sense in terms of sample size and amount of outgassing materials shouldn't really make this an issue.
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
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Name: Beth Dixon Grade/Education Level: High School Location:Rocklin, CA US Email: bdixon-at-rafos.org
Hi Beth This sounds like fun. You can cut hand sections with a razor blade and look at them in brightfield (or fluoresence if you have that ability). Many plant tissues are auto-fluorescent. If you have access to a drawer with stains, you can try staining. Lots of these dyes stain plant tissues differentially and this can simply provide more contrast for you (in either brightfield or fluoresence). It doesn't matter really what exactly they stain (and in many cases that won't be well known).
I am a little curious about the control your student used? Plants do have steriod-type hormones but they are not exactly like those of animals. And certainly some steriod hormones don't do anything to plants. I wonder if there are other materials in the inhaler 'juice' ? I would be happy to correspond with you off-line about that if you like.
Good luck! Tobias
On 5/10/18 8:09 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely } not a member the listserver, and } **any reply should go directly to the poster** } as well as to the list. } Using the "reply" function in your email does *not* send your answer } to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } } } Name: Beth Dixon } Grade/Education Level: High School } Location:Rocklin, CA US } Email: bdixon-at-rafos.org } } Subject: Plant Tissue and Steroid Light Microscopy Ideas } Your Question: } Hello Microscopists! I am a high school biology teacher with a student question. My student has asthma, and her mother, a nurse, had a drawer full of expired inhalers that they wanted to put to use for her 9th grade science project. Using a syringe, they injected plant seedlings with the steroid and monitored their growth. The growth rate of the treated plants was obviously much higher than the non-treated plants, but the student wants to extend her project to see if there are any microscopic differences that she could visualize at the tissue level of the plants using a light microscope. Aside from building stomatal peels, I don't have much experience with plant microscopy, especially not when it comes to hormone detection. Does anyone know of } } } } something she could look for? } Thanks! Beth } } } } } } } ==============================Original Headers============================== } 11, 60 -- From oshel1pe-at-cmich.edu Thu May 10 07:08:05 2018 } 11, 60 -- Received: from NAM03-CO1-obe.outbound.protection.outlook.com (mail-co1nam03on0118.outbound.protection.outlook.com [104.47.40.118]) } 11, 60 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w4AC856G007356 } 11, 60 -- for {microscopy-at-microscopy.com} ; Thu, 10 May 2018 07:08:05 -0500 } 11, 60 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 60 -- d=CentralMichigan.onmicrosoft.com; s=selector1-cmich-edu; } 11, 60 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version; } 11, 60 -- bh=gyiSIErwIZA/F3FJFVPEvmwO5OiszeOEpviCzyV7uIc=; } 11, 60 -- b=Bca3FXtdPTKa8tAI42BktOcctn4KIA9csgtz4ZdPe/XR8epbiL1JrbqpGG3p/PSEyOSafl0cgpAHhUTecQbSz3xYsPLPX0War1rmuZKzrq3W6tsWqvJ465fo9eoR8wZoX4gk3PI9uQ3pkYwR+vA18PUjTwDhp7cKnR9KVihrmiY= } 11, 60 -- Received: from CY1PR05MB2524.namprd05.prod.outlook.com (10.167.10.139) by } 11, 60 -- CY1PR05MB2300.namprd05.prod.outlook.com (10.166.192.146) with Microsoft SMTP } 11, 60 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384) id } 11, 60 -- 15.20.755.10; 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-- __ ___ ^ ___ ___ Tobias I. Baskin / \ / / \ / \ Professor / / / / \ \ \ Biology Department / __/ /__ /___ \ \ \__ University of Mass. / / / \ \ \ 611 N. Pleasant St. / / / \ \ \ Amherst, Massachusetts / /___ / \ \___/ \_____ USA 01003 413-545-1533 bio.umass.edu/biology/baskin BLOG: blogs.umass.edu/baskin/
==============================Original Headers============================== 6, 26 -- From baskin-at-bio.umass.edu Thu May 10 08:46:18 2018 6, 26 -- Received: from marlin.bio.umass.edu (bio.umass.edu [128.119.55.19]) 6, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w4ADkIsr003183 6, 26 -- for {microscopy-at-microscopy.com} ; Thu, 10 May 2018 08:46:18 -0500 6, 26 -- Received: from gouzibyte.bio.mor.nsm (beutopia.bio.umass.edu [128.119.55.10]) 6, 26 -- (authenticated bits=0) 6, 26 -- by marlin.bio.umass.edu (8.14.4/8.14.4/Debian-4.1ubuntu1) with ESMTP id w4ADl8gQ023748 6, 26 -- (version=TLSv1/SSLv3 cipher=DHE-RSA-AES128-SHA bits=128 verify=NO); 6, 26 -- Thu, 10 May 2018 09:47:08 -0400 6, 26 -- Subject: Re: [Microscopy] Re: Ask a Microscopist - microscopic effects on 6, 26 -- plant tissue 6, 26 -- To: oshel1pe-at-cmich.edu, bdixon-at-rafos.org, 6, 26 -- microscnet {microscopy-at-microscopy.com} 6, 26 -- References: {201805101209.w4AC9Ox0007952-at-microscopy.com} 6, 26 -- From: Tobias Baskin {baskin-at-bio.umass.edu} 6, 26 -- Message-ID: {bfe46352-7652-681a-4495-3822b659a420-at-bio.umass.edu} 6, 26 -- Date: Thu, 10 May 2018 09:47:08 -0400 6, 26 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) 6, 26 -- Gecko/20100101 Thunderbird/52.7.0 6, 26 -- MIME-Version: 1.0 6, 26 -- In-Reply-To: {201805101209.w4AC9Ox0007952-at-microscopy.com} 6, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed 6, 26 -- Content-Transfer-Encoding: 8bit 6, 26 -- Content-Language: en-US 6, 26 -- X-Greylist: Sender succeeded SMTP AUTH, not delayed by milter-greylist-4.3.9 (marlin.bio.umass.edu [128.119.55.19]); Thu, 10 May 2018 09:47:09 -0400 (EDT) 6, 26 -- X-Scanned-By: MIMEDefang 2.73 ==============================End of - Headers==============================
Dear colleagues - I am looking for advice and historical references (say prior to 2000) on direct electron beam matter patterning, including beam-induced atom and particle motion, chemical changes induced by electron beam and detected locally via direct atomic imaging or diffraction, beam induced chemical reactions, sculpting, etc. Our group now actively works on harnessing these phenomena for nano- and atomic scale fabrication, and we are extremely interested in past work in these areas. My experience was that many such observations were reported in a very broad spectrum of journals ranging from EM to surface science and applied physics, and very often key words do not allow for effective ISI searches - so I would greatly appreciate pointers to groups and specific papers along those lines.
Thank you in advance!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, Foresight Institute, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 8, 38 -- From sergei2-at-ornl.gov Fri May 11 14:57:54 2018 8, 38 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.137]) 8, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w4BJvrtE012054 8, 38 -- for {microscopy-at-microscopy.com} ; Fri, 11 May 2018 14:57:54 -0500 8, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 38 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 8, 38 -- t=1526068737; x=1557604737; 8, 38 -- h=to:from:subject:message-id:date:mime-version: 8, 38 -- content-transfer-encoding; 8, 38 -- bh=3cVE82F8cGVyccuI0ERUV2ODS5dITktxObnsGIw3lNQ=; 8, 38 -- b=TBrYbhSoJqqnhFQ45xSGfiRTIrwK4Bo/JCNN84BZIyk+0t4KGSdJijo4 8, 38 -- BMfJoy1PMkqKvQOkeyGricHKJOw+wm6W4szXcWYD6c6ZYbFlFlgRbR7V2 8, 38 -- RdTuRW53QcTwomsSqitTqNchFq+Gjb4AYPlYYlyPJyunV4ujai30wyqOe 8, 38 -- 2d0vysrL2FwVdPbml4B9rtr+ixoYNf4C66U+kVnnmO5O1lcYsLexmiQN+ 8, 38 -- cL/KD8asDSuq+7znGm567pEKSIANFonjV+vx/h7eTRu8DM3m85m+VCbya 8, 38 -- sZ2Jp87Hw196GvzMmzFYGO5yt1wL5QmjXRECGj9jmulAGncBWukRITGiP 8, 38 -- g==; 8, 38 -- X-SG: RELAYLIST 8, 38 -- X-IronPort-AV: E=Sophos;i="5.49,390,1520913600"; 8, 38 -- d="scan'208";a="38791901" 8, 38 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 8, 38 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 11 May 2018 15:58:56 -0400 8, 38 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 8, 38 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 38 -- (No client certificate requested) 8, 38 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 40jLWh44Nhz2T4kS 8, 38 -- for {microscopy-at-microscopy.com} ; Fri, 11 May 2018 15:58:56 -0400 (EDT) 8, 38 -- To: microscopy-at-microscopy.com 8, 38 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 8, 38 -- Subject: Historical references - direct electron beam effects on structure of 8, 38 -- matter 8, 38 -- Message-ID: {5AF5F600.5020802-at-ornl.gov} 8, 38 -- Date: Fri, 11 May 2018 15:58:56 -0400 8, 38 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 8, 38 -- Thunderbird/38.0.1 8, 38 -- MIME-Version: 1.0 8, 38 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 38 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Title-Subject: [Filtered] What LIMS are you using for microscopy ?
Message: Hi all,
I'm looking for a laboratory information management system (LIMS) for microscopy. I don't need all the online analysis provided by OMERO, and I'm looking for something simpler. Many of our students just save their data on our server with stupid names like test123. I'm looking for a way to stick a protocol next to every image taken in a clean searchable way. For example, if the image is immunofluo of a bovine embryo, I would want the user to select from a list of protocols, then enter specie, cell type, antibody, wavelength, etc. The actual microscope settings are not so important because they are already stored in the metadata. The best solution would be something open source that we could run locally on our servers.
Many thanks!
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Email: azdiar-at-uow.edu.au Name: Azdiar Gazder
Organization: University of Wollongong
Title-Subject: [Filtered] Available: PhD scholarship at UOW, Australia
Message: A PhD scholarship on the deformation behaviour of a metastable titanium alloy is available at the University of Wollongong, Australia.
For further information, please click on the following weblink: https://eis.uow.edu.au/content/groups/public/-at-web/-at-eis/documents/doc/uow247105.pdf
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Organization: Low Temperature Lab, Centro Atmico Bariloche, Argentina
Title-Subject: [Filtered] Search for MCCB electronic board for FEI XL30 SEM microscope
Message: We are looking for a working MCCB/DBTR electronic board of a FEI XL30\TMP scanning electron microscope (SEM) with motorized stage for our SEM microscope. The non-working board that we have has the labels V5.61 and V2.10 in the board integrated circuits. We kindly appreciate to have news from old microscopes that might have this spare part. This will allow our microscope at Bariloche,Patagonia, Argentina, to be functional again.
Many thanks in advance,
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Email: marilee-at-usc.edu Name: Marilee Reynolds
Organization: University of Southern California Title-Subject: [Filtered] Job Opening at USC Viterbi School of Engineering and Dornsife College of Letters, Arts and Sciences:Senior Research Associate/Bio-EM Senior Scientist
Message: Bio-EM Senior Scientist; Core Center of Excellence in Nano Imaging (CNI)
The USC Viterbi School of Engineering and the USC Dornsife College of Letters, Arts and Sciences is seeking an experienced Bio-Electron Microscopy (EM) Senior Scientist to perform collaborative research and training in a shared instrumentation facility (CNI). This core facility, comprised of electron microscopes and associated sample preparation equipment, serves users from physical and life sciences and engineering (www.usc.edu/dept/CEMMA). The position reports to the Directors of the facility.
The Bio-EM Senior Scientist will collaborate with USC user groups to apply advanced EM methods to address research problems on supported research projects. Process biological specimens for TEM and SEM. Develops protocols for performing advanced EM techniques, including training procedures and licensed user tests. Qualifications: PhD in science or engineering field with at least three years of experience beyond the PhD, demonstrable experience in TEM and SEM techniques, strong EM experience spanning sample preparation to advanced techniques, including FIB, image filtering, EELS, spectroscopic imaging, tomographic imaging, and STEM microanalysis.
Follow this link to the full on-line job announcement. https://usccareers.usc.edu/job/los-angeles/research-associate-senior/1209/7928019
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We are planning to replace the Orius camera with OneView camera on FEI Osiris. The Osiris PC is Window XP. Operation of Orius camera is integrated in the Osiris PC. The STEM imaging /EELS mapping is carried out using TIA (TEM imaging and Analysis) now. Since OneView camera cannot be controlled/operated on the Osiris PC (Windows XP), a separate PC with Window 7 or up will be used for the OneView camera. In this case, Is there a way to use TIA with the OneView camera ? Is it possible to install the TIA software on the PC for OneView ?
STEM imaging / EELS mapping should be OK on the FEI Tecnai Osiris with the OneView camera of.
Please send your experiences and suggestions to xzli-at-unl.edu.
This is to remind you that registration for the BioImage Analysis Workshop hosted by the University of Maryland Baltimore (UMB) Electron Microscopy Core Imaging Facility (EMCIF) on May 24th and 25th will close this Friday, May 18th. Fifteen image analysis experts and software developers will be onsite to demonstrate common workflows for image analysis of biological specimens. The applications that will be discussed during the workshop include Photoshop, ImageJ, Slicer, IMOD, MIPAR, Amira-Avizo, Aivia, Imaris, Arivis, Dragonfly/ORS, and ImagePro and Zen. In most cases, demo software will be available for download. Furthermore, there will be two instrument demonstrations featuring the newly developed 20 Megapixel CMOS TEM camera NanoSprint by AMT, and the robotic automated specimen processor ASP1000 by Microscopy Innovations. Thanks to generous donations by our sponsors, we are able to offer 50 free registrations to graduate student and postdocs. There are still a few spaces left. Please visit the web site below for more information. Website: http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/current-em-techniques-workshop-image-analysis/ Registration: https://umbbioimageanalysis.eventbrite.com/ Inquiries: coreimaging-at-umaryland.edu I look forward to seeing you at the workshop. Sincerely, Ru-ching Hsia rhsia-at-umaryland.edu Associate Professor and Director Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
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Email: azdiar-at-uow.edu.au Name: Azdiar Gazder
Organization: University of Wollongong
Title-Subject: [Filtered] Available: PhD scholarship at UOW, Australia
Message: A PhD scholarship on the deformation behaviour of a metastable titanium alloy is available at the University of Wollongong, Australia.
For further information, please click on the following weblink: https://eis.uow.edu.au/content/groups/public/-at-web/-at-eis/documents/doc/uow247105.pdf
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn
Organization: Stony Brook University
Title-Subject: [Filtered] Artifact
Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the artifact is also present everywhere on the tissue - not on the resin area of the section-only the tissue - mitochondria, nuclei, etc......please comment of what is causing this??? Thank you
Sue
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this reminds me of "salt and pepper"-preciptates due to your fixation / generally processing of the tissue. NB: PB (PO4-buffer, molarity of working solution ??) and too rapid or less careful dehydration (i.e., for example: dehydration out of buffer washes post PFA or PFA/GA or GA with 70% EtOH (for sure 76% EtOH) may cause (sometimes huge) PO4-precipitation within tissue.
Not knowing about your using OsO4 [if yes: in PB too?) as secondary fixative and afterwards....(would be interesting to see your standard processing SOP just to follow all the steps done until examination of the grids)..
Such also would happen also after processing as before + UO2ac. en bloc tertiary fixation (= a pre-staining option) without applying rigorous washing tissue specimens before with the maleate buffer method/sequence to get rid of the whole phosphates.......
Worst case would be (but for sure you are able to dicriminate between microorganisms like bacilli or bacteria from long storage PO4 or other 'ion' precipitation in specimen in primary fix.-solutions) if there happened some detrimental alteration of your kidney specs during storage.
Naturally it would be of benefit to see a typical micrograph/dig. image of the precipitates (i) after conventional double staining (UO2Ac-Lead citrate) as compared to ii) unstained ultrathin sections from same specimen.
Looking forward to further posts (would it be possible to get sent those posts you received as only "personal replies" ?? Thank you in advance!)
To all: Happy Holidays and beautiful, 'inflaming / sparking' Pentecost / Whitsunday,
Our /my thoughts and prayers are with the victims of the most recent rampage -at- Santa Fe High School in Texas and their parents and relatives. May the Lord may have mercy on them!
Respectfully, and With my personal warm regards
Wolfgang
======================================================================== MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired] Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG sterreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 FN-Tel. m. E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 14+ million researchers, including 63 Nobel Laureates)
Former Secretary Long standing Member (until March 2018) and (until June2017) Board Member of the
SCUR {The Society for Cutaneous Ultrastructure Research} The Skin Imaging Society { www.scur.org }
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Samstag, 19. Mai 2018 13:56 An: wij.muss-at-aon.at Betreff: [Microscopy] Artifact in embedded specimen
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Sue
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-------- Forwarded Message -------- Delivered-To: microscopy.listserver-at-gmail.com X-from: Valery Ray {webmaster-at-partbeamsystech.com}
Hi Yanina,
The MCCB/DBTR board is fairly straight-forward to repair, but if you don't have access to decent component lever repair service then there are coupe of MCCB boards available on E*Bay, Item number 173140010597.
Best Wishes and Good luck! Valery
Valery Ray www.linkedin.com/in/valeryray/ Also with REFINE Center, UCONN ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 (leave a message) E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
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The non-working board that we have has } the labels V5.61 and V2.10 in the board integrated circuits. } We kindly appreciate to have news from old microscopes that might have this spare part. This will } allow our microscope at Bariloche,Patagonia, Argentina, to be functional again. } } Many thanks in advance, } } Login Host: 168.96.255.109 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 14, 53 -- From microscopy.listserver-at-gmail.com Sat May 12 06:58:33 2018 } 14, 53 -- Received: from mail-io0-f195.google.com (mail-io0-f195.google.com [209.85.223.195]) } 14, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w4CBwXrC010169 } 14, 53 -- for {microscopy-at-microscopy.com} ; Sat, 12 May 2018 06:58:33 -0500 } 14, 53 -- Received: by mail-io0-f195.google.com with SMTP id f21-v6so9981978iob.13 } 14, 53 -- for {microscopy-at-microscopy.com} ; Sat, 12 May 2018 04:59:38 -0700 (PDT) } 14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=gmail.com; s=20161025; } 14, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 14, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 14, 53 -- bh=Yj1h8T6xtEGn+s5P+FdcXA2ogw+B57QzTogOa2G+d2g=; } 14, 53 -- b=EShMa5KgD+tVzB9EJ1yyb7oPrCNhjtE+6zpzipuBRTqrX1eYvRsbsxO32j+67r0TrB } 14, 53 -- Tk/Nwe7xe+Ca1Oh2dMaFn8t6d0V/gWS1nDe0wHJHUs7N6tQw3gKmfptvmJcV/v5f4eyd } 14, 53 -- qgq4BxW0F+L0yyifE0hicCACKuJKndF6RNkOkWiUsvlVdxLOyEDXnZrYXQ3XEXjc4YRZ } 14, 53 -- pp9kIiAnCT3ZOojUUKfYyp3UU/mEK1zHRDgdrd71+LGyyxMs912BgR1NuwzS6QQ3DDFW } 14, 53 -- 1mrEw973ZpWTzjuws1+kxwFgy3k2pN12PX8RpzH99J5kgEGKA6hFxQnLfE4pcokirhj+ } 14, 53 -- yCZw== } 14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=1e100.net; s=20161025; } 14, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 14, 53 -- :user-agent:mime-version:in-reply-to:content-language } 14, 53 -- :content-transfer-encoding; } 14, 53 -- bh=Yj1h8T6xtEGn+s5P+FdcXA2ogw+B57QzTogOa2G+d2g=; } 14, 53 -- b=iu5NsOKyXTEpyutan5PJ4sB39qxyZR7Znr0bnTVY/D3FEgWP5V43K2MDzlcyM6Qswy } 14, 53 -- iRNrA2tXmF43iNgX3bdBJFaI/zMluZ7Xqo+WbdZLvt6+f9XN4srxJHXkSoTfbAhBQq/C } 14, 53 -- VVjqRtNLocs6WYwUelbZfOaXNbbSq1MHLpULj2HtjVvcHULNIXsFgOWW3EhiFY8rJEY7 } 14, 53 -- DuUVGg9dToFed4QMYwKiTNXBfTPDfTqXD9/p6K7HmOOzjmEW+JWM/XMopnbEXKdpCdN4 } 14, 53 -- PEhWXgBuIYb3BsSkZORQTU9dJKpNFSjg8tp1zmH83IWuUKrCw1iZ16NjcNsyvqZfkoZZ } 14, 53 -- Zrqw== } 14, 53 -- X-Gm-Message-State: ALKqPwf+ydsq1ehacpAXiHtPCyhGxqXdlZCzhF37iOmkVrZcwVJCc3tv } 14, 53 -- jrmlKfW1DMyuIaxfbacSXTKpLpO0 } 14, 53 -- X-Google-Smtp-Source: AB8JxZqr2J+A4PXJRP7IfqxfnnkaDhraj6D0AJZ0FwIvkapmcht8a3z4QaEUGj8qVzjFPiXz+4AqMg== } 14, 53 -- X-Received: by 2002:a6b:10ea:: with SMTP id 103-v6mr2604502ioq.260.1526126377985; } 14, 53 -- Sat, 12 May 2018 04:59:37 -0700 (PDT) } 14, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:a8aa:9564:a409:192]) } 14, 53 -- by smtp.googlemail.com with ESMTPSA id y125-v6sm2505772iod.2.2018.05.12.04.59.37 } 14, 53 -- for {microscopy-at-microscopy.com} } 14, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 14, 53 -- Sat, 12 May 2018 04:59:37 -0700 (PDT) } 14, 53 -- Subject: viaWWW:Search for MCCB electronic board for FEI XL30 SEM microscope } 14, 53 -- References: {201805101724.w4AHOCOu001846-at-microscopy.com} } 14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 14, 53 -- X-Forwarded-Message-Id: {201805101724.w4AHOCOu001846-at-microscopy.com} } 14, 53 -- Message-ID: {f323f7b5-f769-5f64-8d6d-29f21686c1c6-at-gmail.com} } 14, 53 -- Date: Sat, 12 May 2018 06:59:37 -0500 } 14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:52.0) } 14, 53 -- Gecko/20100101 Thunderbird/52.7.0 } 14, 53 -- MIME-Version: 1.0 } 14, 53 -- In-Reply-To: {201805101724.w4AHOCOu001846-at-microscopy.com} } 14, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 14, 53 -- Content-Language: en-US } 14, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - Headers============================== }
You should ask directly FEI for an additional licence (they may decline or charge you for it), but why do you want TIA on the OneView PC? You can acquire images with GMS 3 (Digital Micrograph) and, as you wrote, EELS and STEM will be done with TIA on the old PC.
Cheers, Stefano
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: May 12, 2018 7:33 AM To: Stefano Rubino {stefano-at-soquelec.com}
-------- Forwarded Message --------
X-from: Xingzhong Li {xzli-at-unl.edu}
Dear all,
We are planning to replace the Orius camera with OneView camera on FEI Osiris. The Osiris PC is Window XP. Operation of Orius camera is integrated in the Osiris PC. The STEM imaging /EELS mapping is carried out using TIA (TEM imaging and Analysis) now. Since OneView camera cannot be controlled/operated on the Osiris PC (Windows XP), a separate PC with Window 7 or up will be used for the OneView camera. In this case, Is there a way to use TIA with the OneView camera ? Is it possible to install the TIA software on the PC for OneView ?
STEM imaging / EELS mapping should be OK on the FEI Tecnai Osiris with the OneView camera of.
Please send your experiences and suggestions to xzli-at-unl.edu.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: May 12, 2018 5:33 AM To: Stefano Rubino {stefano-at-soquelec.com}
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X-from: alexandre.bastien-at-fsaa.ulaval.ca
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Title-Subject: [Filtered] What LIMS are you using for microscopy ?
Message: Hi all,
I'm looking for a laboratory information management system (LIMS) for microscopy. I don't need all the online analysis provided by OMERO, and I'm looking for something simpler. Many of our students just save their data on our server with stupid names like test123. I'm looking for a way to stick a protocol next to every image taken in a clean searchable way. For example, if the image is immunofluo of a bovine embryo, I would want the user to select from a list of protocols, then enter specie, cell type, antibody, wavelength, etc. The actual microscope settings are not so important because they are already stored in the metadata. The best solution would be something open source that we could run locally on our servers.
Many thanks!
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-------- Forwarded Message -------- X-from: Nessler, Randy A {randy-nessler-at-uiowa.edu}
Hi Naomi, I have had Bill service our HPF before. His contact info is:
Bill Graham BIBST LABS 37 Averill Road BROOKLINE, NH 03033
603-345-3887 BILL-at-BIBST.COM
Regards, Randy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, May 17, 2018 2:03 PM To: Nessler, Randy A
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X-from: Sara E Miller, Ph.D. {mille012-at-duke.edu}
We sometimes get tissue that has been stored in formalin (not just formaldehyde) after a long period, and while it's not as sharp as with proper fixation, it's fine, certainly diagnostic and without precipitates. Thus, that's not your problem. It sounds like a precipitate of one solution with another. Also, the fact that your spots are on the tissue and not in the resin point to a pre-embedment step. Without knowing your procedure or what the dense spots look like, it would be hard to know where in the procedure this is happening. My guess is that some unbound substance isn't getting washed out properly. Phosphate buffers can make small precipitates in the tissues (not on the resin), and uranyl acetate, if you're using en bloc staining, precipitates with many buffers. Also, if at some point, the surface of the tissue was allowed to dry, that may prevent further washes and fixatives from penetrating thoroughly.
Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Shared Resource P. O. Box 3712 Duke Medical Center Durham, NC 27710
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn
Organization: Stony Brook University
Title-Subject: [Filtered] Artifact
Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the artifact is also present everywhere on the tissue - not on the resin area of the section-only the tissue - mitochondria, nuclei, etc......please comment of what is causing this??? Thank you
Sue
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I ran into this problem several years back. Changed almost every variable that I could - water source, filtration of buffers and fixes, new vendors for chemicals, etc. After an exhaustive search for the culprit I found (through EDS on a new Hitachi TEM) that OSMIUM was the major offender!!! Do you use a post-fix in OsO4? If so, you will find that adding 0.8-1% potassium ferricyanide as a chelating agent may solve your problem. The solution will be bright yellow, like uranyl acetate; it will still act as an oxidizer (tissue will be black at the end of an hour); you may process as usual. It took me forever to figure it out (it seemed like forever anyway.....) and I'm hoping to save you time and aggravation.
Cheers, Debra M. Townley Integrated Microscopy Core Baylor College of Medicine
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, May 19, 2018 6:57 AM To: Townley, Debra
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn
Organization: Stony Brook University
Title-Subject: [Filtered] Artifact
Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the artifact is also present everywhere on the tissue - not on the resin area of the section-only the tissue - mitochondria, nuclei, etc......please comment of what is causing this??? Thank you
Sue
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The UC7 has no place to rest your hands. Nor does it have a calibrated coarse advance wheel. There is also a tendency for the knife holder to collect water, so if you go with the UC7, be sure to keep that area dry overnight or else it will form rust around and over the mirror. When going from an RMC to a Leica, it takes a little getting used to.
On the up side, the Leica has great optics, a very heavy and sturdy base and sturdy attachments. Much less flimsy than the RMC aluminum parts.
The Boss and I argued about Leica vs RMC. I had to give up my precious RMC 6000-XL, which was my favorite microtome ever. I was all for buying a new RMC PC, but that didn't happen. At the time the RMC was about $20,000 cheaper than the Leica, but I see that Leica has come down on their price these days. I really can't compare the 2 since I have not used the RMC, but I sure do like the RMC product.
Good luck with your new tool! Debra
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, May 22, 2018 4:08 PM To: Townley, Debra
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X-from: joseph.mowery-at-ars.usda.gov
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Email: joseph.mowery-at-ars.usda.gov Name: Joseph Mowery
Organization: USDA ARS ECMU
Title-Subject: [Filtered] TEM: Leica UC7 vs RMC Powertome-PC
Message: Hello,
Is there anyone who has used both the Leica UC7 and the RMC Powertome-PC, that could provide some feedback on which is the superior ultramicrotome. The specifications seem very comparable, yet the UC7 seems to be more popular and aesthetically much more attractive. Is the UC7 compatible with an ATUMtome?
The Powertome has flip-up hand rests to stabilize your hands while manipulating sections, does the UC7 have any place to rest your hands?
Any feedback would be greatly appreciated.
Best Regards -Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service Lab 301-504-9027 | Mobile 817-821-8566 joseph.mowery-at-ars.usda.gov
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Title-Subject: [Filtered] Hitachi H-8000 TEM DISSASSEMBLY, assistance needed
Message: I am seeking someone to hire in the vicinity of Columbia, SC that has relevant experience in working with a Hitachi H-8000 transmission electron microscope. I need assistance in disassembling the system to prepare for its removal. Please contact me if you are experienced or available to assist.
Email provenca-at-sccp.sc.edu
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I had a similar problem a few years ago. I contacted the listserve for suggestions and got the following from Ann Ellis. She was a wonderful source of help and information. It worked for me so give it a try.
======================================================= The pepper sounds like the same old thing we have had from time to time over the years with glut and osmium fixation. Traditional buffer washes will not solve the problem. I published a paper way back in Stain Technology (1979) 54:282-285. We ran out of reprints twice since every pathologist and his brother wanted one. I don't have any more or I would send you one. The salvage method is simple. Cut new sections and pick up on nickel grids. Oxidize the sections with 1-2% (wt/vol) freshly prepared periodic acid for 5-10 minutes. Wash the grids several times with deionized water. [with nickel grids you can make the grids wash themselves by setting them on a magnetic stirrer at low speed] Post stain as usual with uranium and lead More importantly, I do have some ideas about how to prevent the problem from happening again. In my many years of doing cytochemical localization, I washed the tissue in buffer wash which contained 0.5-1.0% (vol/vol) DMSO. Thisa removed the aldehyde and protected the enzymatic activity. In the last several buffer washes before localization procedures I added 0.1 M glycine to the buffer wash. This has been recommended for years for removing unbound aldehydes to improve immunolabel. I have never seen the osmium pepper in any of those preps.
A while back I was going back through the list server archives and Randy Tindall had a post about putting a small amount of beta -mercaptoethanol in the buffer washes in an effort to prevent this problem. It probably works similar to the DMSO and glycine.. Ann On 5/25/18, 9:16 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ---------------------------------------------------------------------------- -------- Forwarded Message -------- X-from: Sara E Miller, Ph.D. {mille012-at-duke.edu} We sometimes get tissue that has been stored in formalin (not just formaldehyde) after a long period, and while it's not as sharp as with proper fixation, it's fine, certainly diagnostic and without precipitates. Thus, that's not your problem. It sounds like a precipitate of one solution with another. Also, the fact that your spots are on the tissue and not in the resin point to a pre-embedment step. Without knowing your procedure or what the dense spots look like, it would be hard to know where in the procedure this is happening. My guess is that some unbound substance isn't getting washed out properly. Phosphate buffers can make small precipitates in the tissues (not on the resin), and uranyl acetate, if you're using en bloc staining, precipitates with many buffers. Also, if at some point, the surface of the tissue was allowed to dry, that may prevent further washes and fixatives from penetrating thoroughly. Sara E. Miller, Ph. D. Professor, Department of Pathology Director, Electron Microscopy Shared Resource P. O. Box 3712 Duke Medical Center Durham, NC 27710 Phone: 919 684-9141 Pager: 919 970-8604 Cell: 919 402-3140 Fax: 919 684-3265 CONFIDENTIALITY NOTICE: The information contained in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Medicine policies on confidentiality and/or with the approval of the sender.
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Dear All, may I ask if anyone has actually dismantled and service the Leica Ultramicrotome UCT Ultracut themselves? Recently I start to hear some squeaking sound from the arm when I am trimming at high speed e.g 50mm/s. I guess that the gears are running out of lubricant. However, Leica no longer supports this model and we can't get the service engineer to do the servicing. May I ask anyone has done such servicing themselves and have some tips or guidelines to share?
.. Andrew Anthony Tony Havics, CIH, PE Environmental, Health & Safety, Microscopy, Materials Science & Forensic Engineering pH2, LLC 5250 E US Highway 36, Suite 830 Avon, IN 46123 (317) 718-7020 Office (317) 718-7038 Fax (317) 409-3238 Cell aahavics-at-pH2LLC.com www.ph2LLC.com
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Title-Subject: [Filtered] Cryo-EM facility manager at the Ernst-Ruska-Centre (Forschungszentrum Juelich)
Message: Dear all,
Please find below the announcement for a cryo-EM facility manager position at the Ernst-Ruska-Centre in Juelich (Germany).
The Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C) at the Forschungszentrum Juelich houses some of the world's most advanced electron microscopes and tools for nanocharacterisation. The scientific research covers current issues in condensed matter physics and from now on cryo-EM of biomacromolecules. The ER-C has a total of 13 electron microscopes including the PICO FEI Titan with a point resolution up to 0.5 . The facility will be extended with state-of-the-art cryo-microscopes FEI Talos Arctica and FEI Talos 120.
We are seeking to recruit a facility manager: http://www.fz-juelich.de/SharedDocs/Stellenangebote/_common/dna/2018-153-EN-ER-C%203.html?nn=363560
Please do not hesitate to contact me in case you have any question on the position.
Best wishes,
Carsten ____________________________________________________________________________________ Dr. Carsten Sachse Acting Head ER-C3 Forschungszentrum Jlich GmbH Ernst-Ruska-Centrum, Strukturbiologie 52425 Jlich Germany http://www.fz-juelich.de/er-c/er-c-3/EN/Home/home_node.html
Group Leader European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany phone: + 49 6221 387 8407 email: carsten.sachse-at-embl.de http://www.embl.de/research/units/scb/sachse/ http://www.sachse.embl.deErase this text and type your question here
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Email: renaudgeological-at-execulink.com Name: Jim Renaud
Organization: Renaud Geological Consulting Ltd
Title-Subject: [Filtered] Looking for a JEOL 8900
Message: We are looking for a complete operating JEOL 8900 microprobe. Please let me know if anyone is planning on getting rid of or replacing their probe.
Thanks.
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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD
Organization: Kimberly-Clark
Title-Subject: [Filtered] Inverted Microscope Stand for Donation
Message: Olympus IX70 stand with headpiece, no condenser, no stage Available for donation Excellent condition Image upon request Located in Wisconsin Pickup or paid shipping only Great base for building your own system!
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Hi all, We have a Zeiss 1450EP SEM that is having an issue with the vacuum. It gives a vacuum error message - basically it doesn't want to recognize the vacuum hardware. Has anyone else experienced this problem with their scope? Any advice would be greatly appreciated. If we should just order a black wreath pleaselet us know that, too:)
We took the panels off the scopeand noticed a filter on the side of the scope. It contains anamber-colored substance that we'reassumingis similar todrierite. Does anyone know if we can recharge this filter by drying it in an oven? Can these still be ordered?
thanks for any advice, Beth
==============================Original Headers============================== 4, 54 -- From bethrichardson-at-uga.edu Fri Jun 8 09:41:16 2018 4, 54 -- Received: from NAM04-SN1-obe.outbound.protection.outlook.com (mail-eopbgr700118.outbound.protection.outlook.com [40.107.70.118]) 4, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w58EfGMC000776 4, 54 -- for {microscopy-at-microscopy.com} ; Fri, 8 Jun 2018 09:41:16 -0500 4, 54 -- Received: from DM5PR02MB3259.namprd02.prod.outlook.com (10.164.148.25) by 4, 54 -- DM5PR02MB3718.namprd02.prod.outlook.com (52.132.138.159) with Microsoft SMTP 4, 54 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384) id 4, 54 -- 15.20.841.13; Fri, 8 Jun 2018 14:43:49 +0000 4, 54 -- Received: from DM5PR02MB3259.namprd02.prod.outlook.com 4, 54 -- ([fe80::313e:8490:66da:e61c]) by DM5PR02MB3259.namprd02.prod.outlook.com 4, 54 -- ([fe80::313e:8490:66da:e61c%5]) with mapi id 15.20.0841.015; Fri, 8 Jun 2018 4, 54 -- 14:43:49 +0000 4, 54 -- From: Beth Richardson {bethrichardson-at-uga.edu} 4, 54 -- To: microscopy microscopy {microscopy-at-microscopy.com} 4, 54 -- Subject: Zeiss 1450EP SEM - two questions 4, 54 -- Thread-Topic: Zeiss 1450EP SEM - two questions 4, 54 -- Thread-Index: AQHT/zb/mIfq+LI0BkC2L/UsTibjLw== 4, 54 -- Date: Fri, 8 Jun 2018 14:43:49 +0000 4, 54 -- Message-ID: {DM5PR02MB3259CF1D5632A99EF389EA52DD7B0-at-DM5PR02MB3259.namprd02.prod.outlook.com} 4, 54 -- Accept-Language: en-US 4, 54 -- Content-Language: en-US 4, 54 -- X-MS-Has-Attach: 4, 54 -- X-MS-TNEF-Correlator: 4, 54 -- authentication-results: spf=none (sender IP is ) 4, 54 -- smtp.mailfrom=bethrichardson-at-uga.edu; 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After a fresh reboot of the computer, start your LEO application. Screen print "Shift Prnt Scrn" the LEO-SRV. Post that image to a public web-page. Then repost your query with a link to the screen-dump.
I am looking to see if you get the "L-REM is not responding" or "Vac firmware" message. However, anyone helping you (including Zeiss service) would want to see all the messages.
As for the desiccant on the air line, just ask Zeiss service for the part number. Mine is blue, but we use xdry-N2 for the pneumatics.
regards,
- Jim
PS: OoO count is starting.............
--| Hi all, --| We have a Zeiss 1450EP SEM that is having an issue with the vacuum. It --| gives a vacuum error message - basically it doesn't want to recognize the --| vacuum hardware. Has anyone else experienced this problem with their scope? --| Any advice would be greatly appreciated. If we should just order a black --| wreath please let us know that, too:) --| We took the panels off the scope and noticed a filter on the side of the --| scope. It contains an amber-colored substance that we're assuming is --| similar to drierite. Does anyone know if we can recharge this filter by --| drying it in an oven? Can these still be ordered? --| thanks for any advice, --| Beth
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Email: johnshields59-at-gmail.com Name: John P Shields
Organization: University of Georgia
Title-Subject: [Filtered] Bio TEM workshop
Message: Biological TEM Workshop-- This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and *hands-on* training led by GEM staff. Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Wednesday through Friday, July 25-27, 2018, 8am-5pm each day (lunch is provided) Where: GEM labs, 154 Barrow Hall, University of Georgia, Athens, GA 30602 Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login Deadline: July 20, 2018
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Email: cloehn-at-lsu.edu Name: Clayton Loehn
Organization: LSU
Title-Subject: [Filtered] EM UC7 Ultramicrotome 0.2mm arm retraction
Message: We are experiencing an issue with our Leica EM UC7 Ultramicrotome during the return stroke. The instrument does not seem to be performing the 0.2mm retraction during the return stroke, causing the previous cut sample to be picked back up by the knife edge. Does anyone know what adjustment needs to be made and where (inside the instrument presumably). The instrument seemed to be operating fine after the last PM by Leica, but now is doing the same thing as before the PM. If anyone has drawings, a procedure, or a video that shows how to make the adjustment (or tighten a component), it would be very appreciated Login Host: 167.96.151.109 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: jharris-at-labcor.net Name: John Harris
Organization: LabCor
Title-Subject: [Filtered] Searching for a carbon vacuum evaporator
Message: I'm looking for a another carbon vacuum evaporator in good condition that would be available for sale. Please let me know if you have one in good operating condition that is not in use. Login Host: 96.89.134.209 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We are pleased to inform you that registration for the NexTEM Microscopy Workshop is now open!
The inaugural Next-Generation Transmission Electron Microscopy (NexTEM) Workshop will be held October 8-10 in Discovery Hall at Pacific Northwest National Laboratory in Richland, WA. This event will include three full days of invited and contributed sessions on topics including: · High-resolution imaging and spectroscopy · In situ/operando microscopy in extreme environments · Computational methods for data analysis.
NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions. The workshop will consist primarily of invited talks encompassing state-of-the-art developments and techniques, with a limited number of contributed talks and poster slots also available. These talks will take place during the first half of each day and will be open to all attendees.
An integral part of the workshop will be afternoon breakout and writing sessions, whose attendance will be limited to microscopy domain experts to facilitate active discussion. These sessions will cover the current state-of-the-art emerging advances, and the future of each microscopy sub-field. Importantly, we will encourage the exchange of ideas between breakout groups to identify potential areas of synergy and overlap. Breakouts will be led by two scientists with expertise in each domain who will be tasked with guiding the discussion, as well as outlining and writing. Once we have identified all the participants, we will contact potential breakout leads directly with more information prior to the workshop. We will prepare a perspective review article summarizing major conclusions from the NexTEM Workshop for the Journal of Materials Research in early 2019. All breakout participants will be listed as co-authors on this article.
During registration we are instructing all contributors to submit a two-page M&M style abstract for their talks/posters using the provided template (https://custom.cvent.com/5B9EB96FC2FC4AC69710004DEF407285/files/faf61a705bf64aea8af02becfad67c51.doc). If you have any questions, please don’t hesitate to contact us. We believe this will be an exciting event that will help define the future of electron microscopy and we greatly appreciate your participation.
Visit the NexTEM website (https://pnnl.cvent.com/nextem) for more information, to submit an abstract, and to register!
Workshop Organizers:
Steven Spurgeon Staff Scientist Pacific Northwest National Laboratory
Mitra Taheri Hoeganaes Associate Professor Drexel University
Workshop Coordinator:
Alyssa Cummings Meeting and Event Consultant Pacific Northwest National Laboratory 509-372-4883 nextem-at-pnnl.gov
==============================Original Headers============================== 11, 38 -- From prvs=69460a9a0=steven.spurgeon-at-pnnl.gov Tue Jun 12 11:01:48 2018 11, 38 -- Received: from emailgw02.pnnl.gov (emailgw02.pnnl.gov [192.101.109.63]) 11, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w5CG1lXQ004629 11, 38 -- for {Microscopy-at-microscopy.com} ; Tue, 12 Jun 2018 11:01:48 -0500 11, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 11, 38 -- d=pnnl.gov; s=s20171204; 11, 38 -- h=from:to:subject:date:message-id:content-id: 11, 38 -- content-transfer-encoding:mime-version; 11, 38 -- bh=vQOYV1UlJ2pA4AllF7xaBv6L7Nwevijhe+14LzT3kWg=; 11, 38 -- b=MG3vbVIe+CIFoZBt6nLOZushJCLAHCj8KZ6RWjdN0p/MEny759zv+Oho 11, 38 -- FPQx9B+R5OemsDu3xE9wlSzj+nlfQnPPcMpJPl+OBLc0ZsSUsNCBqEx8W 11, 38 -- t6mX1HbQNaIUJ9Q724dqCOIvJDriFCQX9fxq3AVoDBv61Y4GsKLUPGVSv 11, 38 -- n19wTUSZZgOg5Rl7YEyfJ7vaLKMmw9R3rbljeBYjTEbQnd0jpXV0+wG6z 11, 38 -- /b03ooXrUg7gPy7JK8UhX/g4vZ1Cd/Tm8g6F9G2vLMg+MoaUBTrma3rpq 11, 38 -- MMUH0V/FC0hiFIxXbTaim+PMOA8+5JBfaiiQSXfOJ0OPYG6ZFr/MgveGM 11, 38 -- A==; 11, 38 -- Received: from ex10cashub04.pnnl.gov ([130.20.128.94]) 11, 38 -- by emailgw02.pnnl.gov with ESMTP/TLS/DHE-RSA-AES256-SHA; 12 Jun 2018 09:00:38 -0700 11, 38 -- Received: from EX10MBOX03.pnnl.gov ([169.254.3.2]) by EX10CASHUB04.pnnl.gov 11, 38 -- ([130.20.128.94]) with mapi id 14.03.0399.000; Tue, 12 Jun 2018 09:00:37 11, 38 -- -0700 11, 38 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov} 11, 38 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 11, 38 -- Subject: Registration Now Open for NexTEM Workshop 2018! 11, 38 -- Thread-Topic: Registration Now Open for NexTEM Workshop 2018! 11, 38 -- Thread-Index: AQHUAmaAon6lPMrWH0eWNWHaW3m6Tg== 11, 38 -- Date: Tue, 12 Jun 2018 16:00:36 +0000 11, 38 -- Message-ID: {CB29270E-3D0A-4B4D-99A8-E4F7AB7D62B5-at-pnnl.gov} 11, 38 -- Accept-Language: en-US 11, 38 -- Content-Language: en-US 11, 38 -- X-MS-Has-Attach: 11, 38 -- X-MS-TNEF-Correlator: 11, 38 -- x-originating-ip: [130.20.128.10] 11, 38 -- Content-Type: text/plain; charset="utf-8" 11, 38 -- Content-ID: {72BC4471FBBCE1409A82603255CADADF-at-pnnl.gov} 11, 38 -- MIME-Version: 1.0 11, 38 -- Content-Transfer-Encoding: 8bit 11, 38 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id w5CG1lXQ004629 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both desertrat99-at-verizon.net, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: desertrat99-at-verizon.net Name: Eric Rosen
Organization: UCLA
Title-Subject: [Filtered] Clinical EM Techs. Message: Hiya all, just had a few question for the EM Techs who perform clinical work in hospitals. I have asked this before many years ago and curious to know if things have changed. How many specimens do you process and scope in your lab yearly? How many techs are in the lab? how many scopes do you have in the lab? On a daily basis how many cases do your scope? I will get things started. Over here here we:
Process 1800 cases
2 Techs
Had 2 scopes but one is probably not repairable now
I scope myself about 7-10 a day
lately I scope all the cases in the lab and have done up to 10-15 a day. Login Host: 149.142.103.136 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: aml181954-at-gmail.com Name: Amanda Lawrence
The deadline for early registration is almost here and as you are making plans to attend the Baltimore, MD meetings (Aug. 5-9) please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs and while giving them an opportunity to meet and interact with the established microscopy community. The student bursaries will be paid $10 an hour to work for ~15-20 hours during the meeting and/or pre-meeting events (paid by check at the end of the meetings). The jobs involve such things as providing support in the different symposia, staffing the volunteer office, newsletter distribution, and helping with vendor tutorial sign-up or in the outreach booth. Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities they would like to help with. There is an additional bonus of $10 cash for each morning and/or afternoon session worked to assist with meals and with a meeting t-shirt to wear while serving as a bursary. If anyone would like to participate in the bursary program, please contact the student council (studentcouncil-at-microscopy.org) or Amanda Lawrence (aml181954-at-gmail.com) . Remaining bursary space is limited, so sign-up soon. Participants are responsible for their own registration fee and travel expenses. Don't forget about the Pre-Meeting Congress for students on Sat. Aug. 4. Check with the student council (studentcouncil-at-microscopy.org ) or the MSA website for details.
See everyone in Baltimore,
Amanda Lawrence Student Bursary/Volunteer Coordinator Microscopy Society of America aml181954-at-gmail.com
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X-from: Townley, Debra {debrat-at-bcm.edu} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Hi Clayton,
This won't be of any help to you, just a commiseration. My Leica UC7 is stuck. It won't reset, arm won't retract and knife stage won't retract by using the coarse advance knob. I have 2 red lamps that are winking at me, that's about it. The problem is intermittent and so far, unfixable.
:( Debra Townley Baylor College of Medicine
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, June 11, 2018 5:21 PM To: Townley, Debra
-------- Forwarded Message --------
X-from: cloehn-at-lsu.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MLFormMail.html&d=DwIBAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=U9YNsO3V08vtJQJS0T5thw&m=3slYnxrmpdFe07uffd6nI_10YsKInxI_TUqK5uXQSsc&s=ih1_uJlkjd3eUUyccHIPhDkgAkFqL5o_J3y-tbIZ-10&e= --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both cloehn-at-lsu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: cloehn-at-lsu.edu Name: Clayton Loehn
Organization: LSU
Title-Subject: [Filtered] EM UC7 Ultramicrotome 0.2mm arm retraction
Message: We are experiencing an issue with our Leica EM UC7 Ultramicrotome during the return stroke. The instrument does not seem to be performing the 0.2mm retraction during the return stroke, causing the previous cut sample to be picked back up by the knife edge. Does anyone know what adjustment needs to be made and where (inside the instrument presumably). The instrument seemed to be operating fine after the last PM by Leica, but now is doing the same thing as before the PM. If anyone has drawings, a procedure, or a video that shows how to make the adjustment (or tighten a component), it would be very appreciated Login Host: 167.96.151.109 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both desertrat99-at-verizon.net, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: desertrat99-at-verizon.net Name: Eric Rosen Organization: UCLA Title-Subject: [Filtered] Searching for a Philips (FEI) EM208S Parts
Message: Hi, I am searching for some parts for a Philips (FEI)EM208S. Specifically, looking for a computer for the microscope. It appears ours wants to freeze up after loading the microscope interface.
I am not able to bypass this with a 3.5 inch boot disc to get into a DOS prompt to run some diagnostics.
Anyone out there who has some advice or parts would be greatly appreciated.
Eric A. Rosen Electron Microscopist Dept. of Pathology and Laboratory Medicine UCLA Medical Center 650 Charles E. Young Dr. South Los Angeles, CA 90095
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Email: holpc-at-firstenergycorp.com Name: Chris Holp
Organization: FirstEnergy - BETA Lab
Title-Subject: [Filtered] Camscan SEM vacuum problems
Message: I am looking of assistance for a problem with our Camscan MV2300 SEM. About 17 years ago, the electronics were replaced with Tescan Vega 1 controls. However, the column, chamber, and diffusion pump vacuum system are original.
It has been displaying idiosyncrasies for some time now. Sometimes not venting completely, sometimes pumping down very slowly. I recently replaced both upper and lower solenoid valves with OEM replacements and refilled the diffusion pump with Santovac 5 oil. These steps did help quite a bit but now there is something else.
My current problem is this: after venting, when I push the PUMP button to initiate roughing the chamber out, the solenoid valves sequence, the vacuum draws on the door, but then the butterfly valve opens right away also. I can't help but cringe when I hear the gulp of air surging down the throat into the DP. Left alone, the system does reach good ultimate vacuum, but clearly this situation is very bad and unacceptable. I'm not sure where to look or what to do to get the valve sequencing correct.
Does anyone have familiarity with this scope who can provide some thoughts or advice? Any questions will be readily answered!
Thank you.
Chris Holp FirstEnergy - BETA Labs
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Organization: Centers for Disease Control and Prevention
Title-Subject: [Filtered] Printer
Message: I am in need of a new printer, for B&W prints (biological TEM) and color prints (scanned H&E slides). Your recommendations would be appreciated.
Cynthia Goldsmith
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Dear colleagues, We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the University of Maryland Baltimore will be offering a Biological EM Sample Processing mini-course on July 12th and 13th, 2018 http://www.dental.umaryland.edu/UMBEMProcessingCourse/ . The course is designed to teach any individual who wishes to learn biological sample processing for electron microscopy. No experience is required. The course will be limited to six participants.
--Course format: lectures, demonstrations and hands on practice. --Topics: Fixation, resin embedding, conventional and modern rapid processing methods, microwave assisted processing, automated sample processing, SEM biological sample processing and Negative staining of particulate specimen.
--Other related courses: EMCIF also offers room temperature ultramicrotomy and immunogold labeling minicoures in September and October this year. The minicourses follow a similar format covering principles, practices, and troubleshooting with an emphasis on hands-on practice and instrument demonstration. --More information: Email coreimaging-at-umaryland.edu or visit our website http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/ Thanks.
Sincerely,
Ru-ching Hsia, Ph.D. Associate Professor and Director Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201 Ru-ching Hsia
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The Section of Biology in the Faculty of Sciences at the University of Geneva has a long history in electron microscopy. Indeed, the first Swiss-made electron microscope was built and installed here in the 1940's. Presently, the Section is renewing its Electron Microscopy infrastructure to better support the work of current and future groups. The main function of the facility will be to help users in all aspects of the (Cryo-) EM workflow. To support this facility, we are looking for a
ELECTRON MICROSCOPY PLATFORM SPECIALIST
The specialist will participate in all technical aspects of the facility, including use, maintenance and upkeep of the electron microscopes and associated equipment, support for image processing and some managerial tasks. He/She will be expected to collaborate with users in specimen preparation (cryo-EM or negative stain), operation of the microscopes and associated equipment, instrument training, experimental design, data processing/analysis/storage, interpretation of results, and updating users on the latest methodologies through familiarity with relevant literature. REQUIREMENTS: The successful candidate will have a Ph.D. degree in biology, biochemistry or a related field and must have a minimum of 5 years of hands-on experience in cryo-electron microscopy. He/She must have strong communication skills, be detail-oriented, focused, highly motivated and have the ability to work collaboratively in a team as well as independently on a wide variety of research projects. A strong background in computation would be a plus. English is the working language. ASSIGNMENT: Full-time appointment STARTING DATE: December 1st, 2018, or as agreed Applications should be submitted on-line through our website: https://jobs.unige.ch Complementary information may be by e-mail address (see below)
BIOIMAGING CENTER University of Geneva - Science II Room 245 30, Quai Ernest Ansermet CH - 1211 Genve 4
Dr. Christoph Bauer Managing Director Tel.: + 41 22 3796632 Fax: + 41 22 3796868 email: Christoph.Bauer-at-unige.ch website: http://bioimaging.unige.ch/
==============================Original Headers============================== 8, 44 -- From Christoph.Bauer-at-unige.ch Fri Jun 22 08:24:40 2018 8, 44 -- Received: from mc11.unige.ch (mc11.unige.ch [129.194.9.222]) 8, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w5MDOeo4000940 8, 44 -- for {Microscopy-at-microscopy.com} ; Fri, 22 Jun 2018 08:24:40 -0500 8, 44 -- DKIM-Signature: v=1; a=rsa-sha256; q=dns/txt; c=relaxed/relaxed; d=unige.ch; 8, 44 -- s=20140516; h=MIME-Version:Content-Transfer-Encoding:Content-Type:Message-ID 8, 44 -- :Date:Subject:To:From:Sender:Reply-To:Cc:Content-ID:Content-Description: 8, 44 -- Resent-Date:Resent-From:Resent-Sender:Resent-To:Resent-Cc:Resent-Message-ID: 8, 44 -- In-Reply-To:References:List-Id:List-Help:List-Unsubscribe:List-Subscribe: 8, 44 -- List-Post:List-Owner:List-Archive; 8, 44 -- bh=HpKD1xHMf8mr/Gip6e5RyLLXCVxpPooJksXcirrdZWU=; b=lSy4ZiooiYJW6Bh+vFbD5OYLdX 8, 44 -- 6YCbmqr9FXa3CU4CMZlzGyotpqMe7qMZVto3i2lVtv3N98u5Yrap/voZHI3J32yGerv2eNma+plGs 8, 44 -- n/uS/gdN/tc0rIe9D2myvZhliNu5M0onVhFWtLMYmMNXyZV/jQN91svVf+LauV6e4KdU=; 8, 44 -- Received: from wuniex16-6.unige.ch ([10.13.16.17] helo=wuniex16-6.isis.unige.ch) 8, 44 -- by mc11.unige.ch stage1 with esmtps 8, 44 -- (Exim MailCleaner) 8, 44 -- id 1fWM6Z-000W4X-OJ 8, 44 -- for {Microscopy-at-microscopy.com} 8, 44 -- from {Christoph.Bauer-at-unige.ch} ; Fri, 22 Jun 2018 15:27:59 +0200 8, 44 -- Received: from wuniex16-7.isis.unige.ch (10.13.16.18) by 8, 44 -- wuniex16-6.isis.unige.ch (10.13.16.17) with Microsoft SMTP Server 8, 44 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384) id 8, 44 -- 15.1.1466.3; Fri, 22 Jun 2018 15:27:59 +0200 8, 44 -- Received: from wuniex16-7.isis.unige.ch ([10.13.16.18]) by 8, 44 -- wuniex16-7.isis.unige.ch ([10.13.16.18]) with mapi id 15.01.1466.003; Fri, 22 8, 44 -- Jun 2018 15:27:59 +0200 8, 44 -- From: Christoph Ruediger Bauer {Christoph.Bauer-at-unige.ch} 8, 44 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 8, 44 -- Subject: TEM: Job opening for a cry-TEM specialist at the University of Geneva 8, 44 -- Thread-Topic: TEM: Job opening for a cry-TEM specialist at the University of 8, 44 -- Geneva 8, 44 -- Thread-Index: AdQKK8WUuNzqjJEvRNyGvt0BOqGqJw== 8, 44 -- Date: Fri, 22 Jun 2018 13:27:59 +0000 8, 44 -- Message-ID: {7cd9a1d197d549d199d1c2bdaf44473b-at-unige.ch} 8, 44 -- Accept-Language: en-US, fr-CH 8, 44 -- Content-Language: en-US 8, 44 -- X-MS-Has-Attach: 8, 44 -- X-MS-TNEF-Correlator: 8, 44 -- x-originating-ip: [129.194.52.59] 8, 44 -- Content-Type: text/plain; charset="iso-8859-1" 8, 44 -- MIME-Version: 1.0 8, 44 -- Authentication-Results: localhost; dmarc=none header.from=unige.ch 8, 44 -- Content-Transfer-Encoding: 8bit 8, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w5MDOeo4000940 ==============================End of - Headers==============================
Dear Colleagues - we are hosting two workshops that will run concurrently with the CNMS User Meeting, August 13-15, at ORNL. Registration is free, but advanced registration is required for attendance.
On August 13, we will go through imaging and spectral data analysis through our open-source pyCroscopy platform. This will include tutorials on storing data through hdf5 containers, and performing multidimensional analysis and visualization.
On August 15, we will introduce machine learning methods and show use cases for commonly acquired materials science data. This workshop will also include a short introduction to deep learning.
Both workshops will be hands-on and emphasize use, rather than lectures. We are exploring options for streaming, for those who cannot attend on site. Space is limited! See here for details
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Message: Hello, Does anybody know what are the spherical (Cs) and chromatic (Cc) aberration coefficients of objective lens for an FEI Tecnai G2 20 Twin, operating at maximum 200kV and LaB6 filament? Thank you,
Katayoun
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Title-Subject: [Filtered] Life Sciences Product Specialist
Message: Ted Pella, Inc. is seeking candidates for Life Sciences Product Specialist to develop and grow TEM life science-related products, Histology products, Light Microsopy products and supplies, gold and Nanoparticle lines, and Neuroscientific product lines. For more information please email bonnie_salyer-at-tedpella.com
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The Institute of Materials Science (IMS) at the University of Connecticut (UConn) is an interdisciplinary institute with a threefold mission fostering education, research, and outreach in all areas of the materials sciences. IMS is seeking qualified applicants to fill the position of Microscopy Specialist (Academic Assistant 3) in the new UConn-Thermo Fisher Scientific Center for Advanced Microscopy and Materials Analysis (CAMMA). The Center was formed as a partnership between UConn and Thermo Fisher Scientific to serve the analytical needs of industrial and academic researchers. The Center has acquired seven new electron beam instruments including state-of-the-art TEM, SEM and FIB systems. These are housed in the UConn Technology Park as part of the purpose-built Advanced Characterization Laboratory, which opened earlier this year.
DUTIES AND RESPONSIBILITES Working under the direction of the Center Director, the Microscopy Specialist will have responsibility for the CAMMA activities in the areas of aberration-corrected scanning transmission electron microscopy (STEM), with particular emphasis on in situ experiments and electron tomography. Duties will include: supervising the staff and students who work in the Center; overseeing the operation, maintenance and user training on the instruments; managing systems for user booking and logging of instrument usage.
MINIMUM QUALIFICATIONS An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A minimum of five years’ experience involving the use of aberration-corrected scanning transmission electron microscopes. Good written and verbal communication skills. Strong interpersonal skills including the ability to interact effectively with faculty, staff, students and customers from industry.
PREFERRED QUALIFICATIONS Experience with FEI instruments, including: aberration-corrected TEMs, small dual-beam FIB systems and SEMs. Experience with in situ stages for STEM (heating, gas-cell, liquid cell and biasing). Experience with electron tomography. Strong track record of working with industry.
APPOINTMENT TERMS This is a full time, 11-month position. Salary is commensurate with background and experience and includes a full benefits package.
TO APPLY Please send a letter of application, resume, and the names of three professional references, including contact information via UConn Jobs, http://www.jobs.uconn.edu, Staff Positions. Please reference Search # 2018605 when applying. Screening will begin immediately and this search will remain open until a suitable candidate is found. Employment of the successful candidate is contingent upon the successful completion of a pre-employment background check. (Search # 2018605). This job posting is scheduled to be removed at 11:59 p.m. Eastern time on July 21, 2018. All employees are subject to adherence to the State Code of Ethics which may be found at http://www.ct.gov/ethics/site/default.asp.
The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty and staff. The diversity of students, faculty and staff continues to increase, as does the number of honor students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and instates serve the University’s teaching, research, diversity, and outreach missions, leading to UConn’s ranking as one of the nation’s top research universities. UConn’s faculty and staff are the critical link to fostering and expanding our vibrant, multicultural and diverse University community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities and members of traditionally underrepresented populations.
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Dear Microscopy.com, Please can the following two job adverts be added to the list server. Thanks, Dr. Philip Fletcher, Microscopy and Analysis Suite, University of Bath.
JOB 1: SUBJECT: Instrument Specialist position in Bio Electron Microscopy at the University of Bath Faculty of Science Salary: Starting from 32,548, rising to 38,833 Placed On: Friday 22 June 2018 Closing Date: Tuesday 24 July 2018 Interview Date: To be confirmed Reference: CA5943 Applications are invited for the post of Instrument Specialist in the Material and Chemical Characterisation Facility (MC2). MC2 provides comprehensive microscopy and analytical facilities to researchers across the University. Applicants should have an in-depth understanding and extensive practical experience of electron microscopy (SEM, Cryo-FESEM, TEM), x-ray analysis (EDX) and biological sample preparation methods. The ability to work as part of a team is essential. Duties will involve providing a service to researchers in all aspects of electron microscopy including maintaining and monitoring equipment, and interpreting instrument output (data/images). A state-of-the-art Cryo-FESEM with EDX and STEM has recently been purchased and the instrument specialist will be expected to learn all aspects of the operation of this new instrument. This post is an open-ended contract. Full details of the role itself and the person specifications can be accessed through the link below. http://www.bath.ac.uk/jobs/Vacancy.aspx?ref=CA5943
JOB 2: SUBJECT: Instrument Specialist - BioImaging at the University of Bath Faculty of Science Salary: Starting from 32,548, rising to 38,833 Placed On: Friday 22 June 2018 Closing Date: Monday 23 July 2018 Interview Date: To be confirmed Reference: CA5945 Applications are invited for the post of Instrument Specialist in the Material and Chemical Characterisation Facility (MC2). MC2 provides comprehensive bio-imaging facilities to researchers across the University. Applicants should have an in-depth understanding and extensive practical experience of confocal laser scanning microscopy (CLSM) and fluorescence activated cell sorting (FACS). Experience of working with a CLSM plus a multiphoton laser and/or a high content microscope would be an advantage. The ability to work as part of a team is essential. Duties will involve providing a service to researchers in CLSM, FACS, and use of hypoxic facilities including maintaining and monitoring equipment, and interpreting instrument output (data/images). A state-of-the-art CLSM with multiphoton laser has recently been purchased and the instrument specialist will be expected to learn all aspects of the operation of this new instrument. This post is an open-ended contract. Full details of the role itself and the person specifications can be accessed through the link below. http://www.bath.ac.uk/jobs/Vacancy.aspx?ref=CA5945
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Title-Subject: [Filtered] Job Posting: Cellular Biologist / Cell Structure Message: Dear colleagues, We are currently seeking a Cellular Biologist / Cell Structure Expert for a position within the Laboratory of Cell Structure and Dynamics at the NIDCD, NIH.
Research area: Structure and regulation of stereocilia and other model actin-based cellular protrusions
For details about the position and to apply visit: https://kelly.secure.force.com/CandidateExperience/CandExpJobDetails?id=a7V80000000UM3VEAW&searchFlag=true&tid
For details about the Laboratory and research program please visit: http://www.nidcd.nih.gov/research/scientists/pages/kacharb.aspx
For further inquiries please contact me directly. Best regards, Bechara ---------------------------------------------------- Bechara Kachar, M.D. Chief, Laboratory of Cell Structure and Dynamics NIDCD, National Institutes of Health Porter Neuroscience Research Center 35A Convent Drive, Room 3D-824 Bethesda, MD 20892 Tel.: 301-402-1600 Email: kacharb-at-nidcd.nih.gov
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Email: steven.cogswell-at-gmail.com Name: Steven Cogswell
Organization: UNB
Title-Subject: [Filtered] Looking for Mag Poweramp for JSM-6400
Message: Hi folks;
My venerable jeol jsm-6400 (tungsten) sem has developed some nasty image distortions (crt and recorded with digiscan), and we think it's probably our good friend the mag pwramp box. I'm trying to get a replacement for it, and jeol does not have one. This is an open-frame assembly board but it's in the gold-box-waterblock in the bottom of the instrument. My schematic book lists it as 806512822. It's probably in a lot of different jsm machines. I can provide more details if you want it. Do any of you nice folks have one you're willing to part with?
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after setting up a Jeol 820 SEM at a German highschool I do have the following problem:
- no higher acceleration voltage possible than 2.9 kV
Switching to 3kV on the digi switches causes the emission to become unstable and fluctuating in a very large range.
Below 3kV all is fine.
HV tank itself seems to be OK. I had it open and nothing appeared to be out of position.
HV cable is also OK. The problem is nearly the same without cable (fluctuations are existing, but since no emission, only ca. 10% of former value...).
I suspect that either the board logic becomes flawed after using the 3kV setting. It seems like the HV changing values very fast but there is no direct readout to check for. Or the bias. I am not sure.
Maybe somebody in the group has a HV tank available or the HT generator board, Jeol number AP00188(01) ?
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: unocicrr-at-ornl.gov Name: Raymond Unocic
Organization: Oak Ridge National Laboratory
Title-Subject: [Filtered] Microscopy and Microanalysis EMLG FIG
Message: Microscopy and Microanalysis Electron Microscopy in Liquids and Gases Focused Interest Group
Dear M&M Attendees:
On behalf of the EMLG FIG we would like to bring to your attention to two events for researchers that are interested in the field of in situ/operando microscopy in liquids and gases:
1) Pre-meeting Congress X62 meeting (Practical Challenges and Opportunities for in situ/operando microscopy in liquids and gases) that will be held on Sunday August 5, 2018 at the Annual M&M2018 meeting in Baltimore Maryland. Please come hear about the latest advances in this field from an exciting lineup of invited speakers including:
Bryan Reed (IDES, inc.), See Wee Chee (NUS), Katherine Jungjohann (Sandia National Laboratory), Alex Belianinov (Oak Ridge National Laboratory), Eric Stach (University of Pennsylvannia), Lena Kourkoutis (Corness), Alex Liddle (NIST), Xiaoqing Pan (UC Irvine), Renu Sharma (NIST), Stephen Mick (Gatan) Registration cost: $159 (Members), $209 (Non Members), $75 (Students)
2) Our EMLG FIG will also hold our business meeting on Tuesday August 7, 2018 at 12:15 p.m. (Room TBD) Our leader elect (Dr. Houlin Xin) will be assuming leadership responsibilities for 2018-2020 and will be holding an election for our leader elect position (who will take over the FIG in 2020) and sec/treasurer. In order to attend the meeting and be eligible to vote, it is important to have your $10 membership dues paid in full. The membership fees can be paid through your MSA account or when you register for the conference. See you in Baltimore :) Ray Unocic
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Email: tzega-at-lpl.arizona.edu Name: Tom Zega
Organization: University of Arizona
Title-Subject: [Filtered] Associate Staff Scientist to manage FIB laboratory at U Arizona
Message: Colleagues,
I'd like to draw your attention to an open position for Associate Staff Scientist at the University of Arizona to manage a FIB and SEM laboratory.
} From the job description:
The Kuiper Materials Imaging and Characterization Facility (KMICF) at the University of Arizona invites applications for the position of Associate Staff Scientist. KMICF is part of a network of core analytical facilities and is dedicated to imaging, spectroscopy, and analysis of heterogeneous materials. Laboratories include electron microprobe, scanning electron microscopy, transmission electron microscopy, and focused ion beam electron microscopy. The staff scientist will work with the instrument scientist and will be responsible for managing daily operations of the focused-ion-beam and scanning electron microscope laboratories.
Please visit https://uacareers.com/postings/30631 for more details and to apply.
Thank you.
Tom Zega
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X-from: Bill & Sue Tivol {wtivol-at-sbcglobal.net}
} On Jun 15, 2018, at 4:23 PM, microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} wrote: } } Message: I am in need of a new printer, for B&W prints (biological TEM) and color prints (scanned } H&E slides). Your recommendations would be appreciated. } Dear Cynthia, Some time ago a contributor to the list recommended the Epson Stylus C88+, which I subsequently bought and am very happy with. It may no longer be available, but perhaps its successor is equally good. Yours, Bill
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Email: pierceem-at-ornl.gov Name: Eric M. Pierce
Organization: Environmental Sciences Division/Oak Ridge National Laboratory
Title-Subject: [Filtered] Job Posting for Postdoctoral Research Associate, Environmental Biogeochemistry / NB50639999
Message: Company: The Environmental Sciences Division, http://www.esd.ornl.gov, at Oak Ridge National Laboratory (ORNL), http://www.ornl.gov, is seeking to fill a postdoctoral position in the area of environmental biogeochemistry. The project is aimed at understanding the interfacial processes between mineral surfaces, trace metals, natural organics, and microorganisms. You will be involved in a multidisciplinary research team investigating coupled geochemical, biological, and environmental processes that influence the chemical speciation, biological uptake and transformation of contaminants (such as mercury) in water and sediments. Particular interest is the application of high-resolution imaging tools to improve our understanding of processes that influence trace metal speciation at the interfaces between minerals-microorganisms and minerals-organic matter. You should have experience necessary to initiate laboratory studies, which include, but are not limited to, developing or refining research ideas, setting up laboratory experiments, collecting experimental data, data analysis, writing journal publications, and presenting at group and scientific meetings. Experience with the use of high-resolution microscopy tools in the context of biogeochemistry or material science is a plus but not required. The selected candidate will work closely with Earth Sciences Group leader, other Earth Sciences group members, and also collaborate closely with other scientist at ORNL and collaborating institutions.
Major Duties/Responsibilities: You will have the responsibility to design and conduct laboratory experiments, maintain detailed scientific records, analyze experimental data, present results within the research team and at national and international conferences, and prepare manuscripts for publication in peer-reviewed journals. The nature of our scientific work is highly multidisciplinary, and projects will be carried out in close collaboration with scientists from a variety of scientific backgrounds, including geochemistry, microbiology, biochemistry, physical chemistry, or a related field to investigate the biochemistry of contaminant transformations.
Qualifications Required: PhD in environmental chemistry, geochemistry, geomicrobiology, physical chemistry, material science, or related discipline. Experience with advanced analytical microscopy techniques, such as electron microscopy and electron energy loss spectroscopy, is highly desired You cannot have received the most recent degree more than five years prior to the date of application and must complete all degree requirements before starting your appointment. You should demonstrate the ability to work independently and collaboratively as part of a large, multidisciplinary team and should be able to design and execute experiments, perform data analyses and interpretation of experimental results. Strong record of productive and creative research demonstrated by publication in peer-reviewed journals and presentations at scientific conferences, and experience with environmental biogeochemistry or material science are expected. Excellent interpersonal, oral, and written communication skills are required.
Appointments will initially be for 24 months with a possibility of an extension of up to 12 months. Initial appointments and extensions are subject to performance and availability of funding.
Relevant Websites: ORNL Earth Sciences Group: https://www.ornl.gov/division/esd/earth-sci ORNL Environmental Sciences Division website: https://www.ornl.gov/division/esd Primary Research Program website: https://www.esd.ornl.gov/programs/rsfa/
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With a heavy heart, I write this announcement that on Friday, June 29th, at 9:01 PM, the co-founder and namesake of Ted Pella, Inc., Ted Pella, died and went to glory. He died on his 91st birthday from complications due to Alzheimer's Disease, at Oakmont of Redding Retirement & Assisted Living Facility. He died with his wife Christel at his side.
He was preceded in death by his daughter Stephanie Pella Mills and his grandchild Christen Joy, and is survived by his wife Christel; his sister Alice Pella Prong; his son Thomas and daughter-in-law Diane; and his three grandchildren, Claire, Cornelia and Cameron.
Ted was born on June 29th, 1927 to the late Rudolf and Mary Pella in East Rochester, NY. Ted's character and spirit were molded by the kindness of his mother, a devout Catholic. He grew up in the Great Depression and while his family was poor, he never knew that, enjoying the simplicity of being around family and friends. While growing up he worked in the family grocery store. It was there that Ted's passion for serving his customers was first planted and became a guiding principle wherever he worked.
Ted was a starter on the High School basketball team, was voted most likely to succeed, and graduated High School as the valedictorian of his class. But he skipped the ceremony and instead signed up for the Navy early in the summer of 1945. The war was ending, however, and the closest he got to the front lines was working in a discharge center in Memphis, TN.
His brief time in the service entitled him to attend college, and he returned home to attend the University of Rochester, getting a Bachelor's degree in Physics in 1950. Upon graduation he moved to New York City and found temporary quarters at the YMCA. Shortly afterwards he saw a job posting there on an index card for a Sales Engineer for the Ivan Sorvall Company which was designing and selling laboratory instrumentation. This was a perfect fit for Ted, who was looking for something that combined business and science.
He went to work in field sales and service for Sorvall. Ted delivered the first commercial ultramicrotome ever sold, under his arm to the Rockefeller Institute in New York, taking a train to get there. He greatly enjoyed visiting with customers, helping repair instruments in their labs and finding ways to help. Not long after joining Sorvall, Mr. Sorvall passed and the company was bought by LKB, another scientific instrument company, based out of Sweden. Ted continued his sales work for LKB and began to rise in the ranks until he oversaw sales for the USA.
It was during this period in 1956 that LKB sent him to Sweden for further instrument training. The person conducting the training was a TEM Technician at Karolinska Institute named Christel, and thus began a relationship which led to Christel emigrating to the USA, and their getting married in 1957. They were married for 60 years, celebrating that anniversary last year.
Ted was eventually promoted to VP of Sales at LKB, and the family moved to Sweden in 1966. But Ted was not a good fit for the Swedish culture and the home office. During a vacation Ted bounced the idea off Chris: Why don't we start our own business back in the states? Chris had a spirit of adventure and they agreed to start it together. The family moved back to the states in late 1967, this time to Southern California where on Jan. 1, 1968 Ted Pella, Inc. was started.
The business started in their garage and den. They soon hired their first employee at home to help type; and a few years later moved into their first office space. The company slowly grew and added employees, and they worked many hours to make ends meet and meet the needs of their customers.
The time finally came when they wanted to own their own building; but this was the time of the great real estate boom of the 1980's and no land was easily available nearby in Orange County for building. The City of Redding, California eventually "found" them at a trade show in 1986 and introduced them to what Northern California had to offer. Ted & Chris moved the company to Redding in the Spring of 1987. At that time the number of employees had grown to 19.
Ted & Chris continued to own and manage the company for 44 years, until 2010 when the number of employees was over 60. During this time the company grew to one of the premier suppliers of microscopy equipment and supplies worldwide. But Ted and Chris always kept their focus on serving the needs of scientists. In 2010 they passed ownership and management to their son Tom, but continued to be involved into their mid 80's until they finally retired in 2014, having served the microscopy community for over 60 years. Even at the 2017 Neurosciences show a number of customers asked how Ted was doing, having enjoyed meeting him at the booth in a trade show.
The company will observe a special half-day holiday in Ted's honor and close tomorrow, Wednesday, July 11th, in the afternoon to hold a Memorial Service and reception at the Dignity Memorial McDonald's Chapel in Redding for employees and friends. If anyone wishes to express their condolences to Christel and post a note about Ted for that event, please send them to tom_pella-at-tedpella.com and it will be included in an album being presented later to Christel.
X-from: Phillips, Thomas E. {PhillipsT-at-missouri.edu} CC: alexandre.bastien-at-fsaa.ulaval.ca {alexandre.bastien-at-fsaa.ulaval.ca}
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X-from: alexandre.bastien-at-fsaa.ulaval.ca
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Title-Subject: [Filtered] What LIMS are you using for microscopy ?
Message: Hi all,
I'm looking for a laboratory information management system (LIMS) for microscopy. I don't need all the online analysis provided by OMERO, and I'm looking for something simpler. Many of our students just save their data on our server with stupid names like test123. I'm looking for a way to stick a protocol next to every image taken in a clean searchable way. For example, if the image is immunofluo of a bovine embryo, I would want the user to select from a list of protocols, then enter specie, cell type, antibody, wavelength, etc. The actual microscope settings are not so important because they are already stored in the metadata. The best solution would be something open source that we could run locally on our servers.
Many thanks!
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We are seeking a Postdoctoral Research Associate to support our efforts in Scanning Transmission Electron Microscopy and Scanning Probe Microscopy Groups on the mathematical aspects of physics extraction from spatially resolved imaging of dynamic systems. Our team aims to establish a comprehensive means of feature extraction, conversion of dynamic imaging data to materials specific descriptors, and recovery of underpinning physical behaviors. As a postdoc, you will help us transition from the atomically-resolved and mesoscopic scanning probe and electron microscopy data to underlying physics laws, via either mesoscopic dynamic models or atomistic dynamics. You will be collaborating with an interdisciplinary research team to develop machine learning methods for rapid, on-the-fly analytics of imaging and spectral imaging data, and then to implement these methods and algorithms for physical analysis.
This position resides in the Center for Nanophase Materials Sciences Division (CNMS), Physical Sciences Directorate (PSD) at Oak Ridge National Laboratory (ORNL).
In this position you will: • Develop and adapt algorithms and software for materials specific feature extraction from atomic and mesoscopic imaging • Develop approaches for physics extraction from imaging data using inference, information compression, manifold learning, representation learning, and similar methods • Collaborate with team members within and outside of electron, scanning probe, and other imaging groups at ORNL • Present and report research results and publish scientific results in peer-reviewed journals
Basic Qualifications/ To be considered you must have: • A PhD in Computer Science and Engineering, Applied Mathematics, Computational Physics, or closely related field completed within the last five years • A track record of excellent analytical and problem solving skills • Experience with Python and the associated scientific software library stack • A strong record of productive and creative research demonstrated by publications in peer-reviewed journals and/or presentations at scientific conferences
Preferred Qualifications/ This experience is to your advantage: • Background in data analysis and simulations using Python or Matlab • Excellent written and oral communication skills and the ability to communicate in English to an international scientific audience • Motivated self-starter with the ability to work independently and to participate creatively in collaborative and frequently interacting teams of researchers • Ability to function well in a dynamic research environment, set priorities to accomplish multiple tasks within deadlines, and adapt to ever-changing needs
Contact Sergei Kalinin (sv9-at-ornl.gov) for additional information.
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, FPresight Institute, IEEE, APS, IoP, AVS
I am seeking advice and suggestions on how to keep nital chemical solution (33%nitric acid in methanol) in the lab. We use this solution quite often to make metal TEM samples. However, the university safety officer does not allow us to keep it in the lab for future use. They requested us to dispose it after one time use. We never had issues with the old solutions. We are trying to persuade the safety office to let us keep this solution in the lab for at least several months. I would really appreciate your input and suggestions and the protocol on how your lab handle and store this solution.
I agree with your safety officer. 33% Nitric in Methanol is an unstable explosive mixture. Nitric acid can react with methanol to produce methyl nitrate which is a colorless volatile liquid that is explosive.
Typically any solution of Nital (nitric acid/ alcohol) over 5% nitric should not be stored.
I saw the results of this many years ago while a graduate student. Even stored in a fridge a bottle of 30% nitric in methanol exploded overnight.
Regards
Alan
Alan Nicholls, PhD Associate Director, RRC Director, Electron Microscopy Service Research Resources Center The University of Illinois at Chicago 845 West Taylor Street 110, SES, MC337 Chicago, IL 60607 (312) 996-1227 nicholls-at-uic.edu
-----Original Message----- X-from: xin-at-magnet.fsu.edu [mailto:xin-at-magnet.fsu.edu] Sent: Thursday, July 12, 2018 2:31 PM To: Nicholls, Alan W {nicholls-at-uic.edu}
Dear Colleagues,
I am seeking advice and suggestions on how to keep nital chemical solution (33%nitric acid in methanol) in the lab. We use this solution quite often to make metal TEM samples. However, the university safety officer does not allow us to keep it in the lab for future use. They requested us to dispose it after one time use. We never had issues with the old solutions. We are trying to persuade the safety office to let us keep this solution in the lab for at least several months. I would really appreciate your input and suggestions and the protocol on how your lab handle and store this solution.
Colleagues, Does anyone have a user's manual for the RMC MF-7200 Cryo Propane Jet Ultrarapid Freezer? Or spare parts for that instrument they would be willing to loan, give, or sell, including but not limited to, the control cable, a sample holder, a sample port plug, perhaps hoses/connectors for N2 gas and liquid nitrogen? I am posting this on behalf of my colleague Larry Winship, who is not a member of the list. Please reply to him ljwNS at hampshire.edu.
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Title-Subject: [Filtered] job offer: sales representative in Ontario
Message: Start date: as soon as possible
Hourly/yearly wage: competitive, to be discussed
Job type: Permanent full-time
Relocation/travel requirements: position based in Toronto/Ottawa with up to 80% travelling
Soquelec Ltd., founded in 1974, is the Canadian distributor of consumables and equipment for high-end imaging, analysis, and sample preparation research laboratories across Canada.
Job Summary
The ideal candidate would be responsible for the sales and, possibly, service of scientific equipment from a variety of suppliers (TESCAN, Gatan, Quorum, Bruker, etc.), as well as establish and maintain strong business relationships with our current and potential customers in Ontario and the Maritime provinces. You will be working with the rest of the sales team and the administrative staff closely to achieve your sales goals and contribute to company objectives.
Key Responsibilities
Initiate contacts with potential customers and qualify sales opportunities; Follow up and close sales opportunities in a timely manner; Update internal CRM regularly of any relevant task, activity or note; Train end users on applicable instruments; Build and facilitate positive and productive customer relationships; Participate in application trade shows and conferences; Participate in applicable training opportunities.
Requirements
Bachelors Degree in Science or Engineering; Excellent communication skills in English; Prior experience in electron microscopy and/or x-ray imaging; Excellent sales ability and customer focus; Strong self-management skills and initiatives; Drivers license; Willingness and readiness to travel 80% of the time.
Assets
Prior sales/customer service experience; Working knowledge of French.
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Title-Subject: [Filtered] Problems with cryo-sectioning
Message: Dear All
lately I'm having problems with cryo-sectioning, more precise picking up the sections. The sectioning is fine and I'm getting nice sections (samples embedded in 10% gelatin). However, when I'm picking them up (with 1:1 methylcellulose/ 2M sucrose) with the loop, the sections don't seem to attach to the grids (formvar coated ones). I have made new grids, gelatin, sucrose and methylcellulose; but so far I'm always ending up with 1-2 sections in one single grid (out of 10 collected grids). Any ideas or thoughts would be greatly appreciated.
All the best from (currently not so cold) Scotland
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Title-Subject: [Filtered] EMS Microscopy Academy October Workshops
Message: Don't miss out! Sign up for one these October workshops today!
Cryosectioning/Immunogold Workshop Led by Peter van de Plas, Helmut Gnaegi and Michael Kostrna
Monday - Friday October 8 - 12, 2018 Hatfield, Pennsylvania, USA
Five days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in cryosectioning and immunogold labeling Will provide researchers with the opportunity to learn the theory and practice of the use of cryosectioning with diamond knives, and immunogold labeling.
Cryo SEM Workshop Led by Al Coritz and Michael Kostrna
Tuesday - Thursday October 23 - 25, 2018 Hatfield, Pennsylvania, USA
This course will cover the process of rapid freezing, fracturing, coating and imaging of a variety of samples. For individuals who are new to the field of cryo SEM or desire a technical refresh to maintain current skills or just those that want to see and learn all of the possibilities of the technology.
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Title-Subject: [Filtered] Zeiss 10CA TEM spare parts
Message: All: I have a decommissioned Zeiss 10CA TEM that will be removed from my lab. Does anyone need spare parts? Login Host: 69.140.136.120 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: scottpj-at-vt.edu Name: Philip Scott
Organization: Virginia Tech
Title-Subject: [Filtered] Opinions and Experience with K-kit for Liquid TEM?
Message: Hello!
I am interested in observing some polymer particles (150 nm) in aqueous media without drying for conventional TEM. I found the Ted Pella K-kit for Liquid TEM which looks perfect for my needs as my university does not have a liquid cell holder for our TEM's. Before purchasing this product, I would like some insight into the details and problems (if any) with using it. For those of you with experience with this product, would you recommend it?
Also what is the resistance of the sealant/glue to organic solvents?
Thank you! Phil Scott scottpj-at-vt.edu
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Please consider submitting an abstract to the following session at the 2018 AGU Annual Meeting in Washington, D.C., on December 10-14, 2018: P038: Revealing the origins of our Solar System and its organic compounds through analysis of meteorites and related planetary materials
Session Description: Laboratory studies of cosmic dust, meteorites, asteroids, and comets expand our understanding of the chemical and geophysical origins of our Solar System. Such studies provide essential constraints for geophysical models of planetary nebulae, accretion and differentiation of planetesimals, and the origin and diversity of organic compounds. Furthermore, comprehending the organic complement of extraterrestrial objects can help distinguish false positives when searching for indigenous biosignatures. This session invites papers related to the analysis of meteorites and other similar planetary materials that advance our understanding of the origin and evolution of our Solar System and extraterrestrial organic compounds. Topics include radiative and geochemical processes active in the early solar nebula; the timing, location, and genetics of planetary accretion and differentiation; connecting astronomical observations with laboratory data of meteorites, returned samples, and analog materials; and the origin and evolution of organic molecules found on asteroids, comets, and their fragments.
Invited Speakers: Benjamin Weiss (Massachusetts Institute of Technology) Hiroshi Naraoka (Kyushu University)
Conveners: Eric Parker (NASA Goddard Space Flight Center) Bradley De Gregorio (U.S. Naval Research Laboratory) Emma Bullock (Carnegie Institution of Washington) Heather Graham (NASA Goddard Space Flight Center)
More information about this session can be found at https://agu.confex.com/agu/fm18/prelim.cgi/Session/54516 We are also working with American Mineralogist to create a Special Section for the journal, focusing on research topics presented in our session! Please consider how your abstract could be expanded into a full length research paper. Dont wait too long to submit your abstract. The abstract submission deadline is August 1st, 23:59 EDT. We look forward to your contribution!
About AGU: For the first time, the AGU Fall Meeting will be held in Washington, D.C., where we will mark the launch AGUs Centennial. A wide variety of events are being planned that will take advantage of this special location that will showcase our science to the U.S. and international policy community, students, and public); leverage the local scientific community, including events with the Smithsonian, National Academies, and others; and, offer field trips to view the local geology and research institutes. The Fall Meeting will also offer more workshops as well as new Tutorial sessions to help students and researchers learn about new approaches and techniques and introduce exciting science in other disciplines. Additional information, including everything you need to know about abstract submission is here: https://fallmeeting.agu.org/2018/. Please consider submitting an abstract and/or attending to help show What Science Stands For and to join in an exciting and informative start to AGUs Centennial. Submissions received by the early deadline of 25 July have a chance to win a $100 US gift card.
Bradley T. De Gregorio Geologist, Code 6366 Materials Science & Technology Division T (202) 767-6294 F (202) 767-1697
www.nrl.navy.mil
==============================Original Headers============================== 11, 23 -- From bradley.degregorio-at-nrl.navy.mil Tue Jul 24 10:22:47 2018 11, 23 -- Received: from ccs.nrl.navy.mil (mx0.ccs.nrl.navy.mil [132.250.118.211]) 11, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w6OFMlZF028807 11, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jul 2018 10:22:47 -0500 11, 23 -- Received: from bassimnrl (bassimnrl.nrl.navy.mil [132.250.126.175]) 11, 23 -- by ccs.nrl.navy.mil (8.14.4/8.14.4) with ESMTP id w6OFRq6w019708 11, 23 -- for {microscopy-at-microscopy.com} ; Tue, 24 Jul 2018 11:27:52 -0400 11, 23 -- From: "Bradley De Gregorio \(Code 6366\)" {bradley.degregorio-at-nrl.navy.mil} 11, 23 -- To: {microscopy-at-microscopy.com} 11, 23 -- Subject: =?iso-8859-1?Q?Call_for_abstracts_-_Planetary_Science_Session_P038_at_AGU?= 11, 23 -- =?iso-8859-1?Q?_2018?= 11, 23 -- Date: Tue, 24 Jul 2018 11:27:51 -0400 11, 23 -- Message-ID: {005501d42362$e24c37c0$a6e4a740$-at-nrl.navy.mil} 11, 23 -- MIME-Version: 1.0 11, 23 -- Content-Type: text/plain; 11, 23 -- charset="iso-8859-1" 11, 23 -- X-Mailer: Microsoft Outlook 15.0 11, 23 -- Content-Language: en-us 11, 23 -- Thread-Index: AdQjYqATqJSQ8MZ/SHGxoNZdQgtctw== 11, 23 -- X-CCS-MailScanner: No viruses found. 11, 23 -- X-CCS-MailScanner-Info: See: http://www.nrl.navy.mil/ccs/support/email 11, 23 -- Content-Transfer-Encoding: 8bit 11, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w6OFMlZF028807 ==============================End of - Headers==============================
After many years we are finally releasing our 1979 vintage Jeol 733 Microprobe.
JEOL JXA-733 Superprobe equipped with four wavelength-dispersive X-ray spectrometers, an Link/Oxford energy-dispersive system consisting of a detector and analysis software, and Advanced MicroBeam automation.
If you have an interest is this system please contact me offline at Email below.
Roy Beavers Southern Methodist University Department of Earth Sciences 3225 Daniel Ave Dallas TX 75205 Voice: 214-768-2756 Email: rbeavers-at-smu.edu
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Email: ugez323-at-yahoo.co.uk Name: Jonathan
Organization: UCL
Title-Subject: [Filtered] Biorad Lasersharp 2000 and Win 7.
Message: Hi
We are trying to keep our Bird confocal running and wondered if the software could run on Windows 7? Has anyone managed to do this?
It seems it might be possible. We gave it a go and on starting the software the confocal head clicks so it seems to be talking to it, but the software then comes up with an error (.ocx file not registered). Tried to register it manually but it won't do it.
Any pointers appreciated!
Thanks
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I would like to inform you that Electron Microscopy Core Imaging Facility (EMCIF) of University of Maryland Baltimore (UMB) will host a pre-M&M open house and instrument demo on August 4th and 5th (Sat & Sun) from 10 AM to 4 PM each day. All our sample preparation equipment, scopes, and some vendor supplied instrument will be available for try out. The purpose of this open house is to provide an opportunity for the general public to tour a multifunctional EM Facility and for EM professionals to test small equipment designed to enhance workflow and efficiency in modern EM Facilities. In particular, these include the PELCO BioWave Pro+ from Ted Pella; GloQubeTM, Lynx II tissue processor from EMS; ASP1000 automated EM sample processor from Microscopy Innovations; and EM GP from Leica Microsystems, etc.
UMB is located in downtown Baltimore, within walking distance from the Baltimore Convention Center, Camden yard, Edgar Allen Poes Cemetery. There will be light refreshment served all day, so please drop in while you are touring Inner Harbor and Baltimore City. Walk-ins are welcome. Kids and Family members are also welcome. You have the option to signup ahead to reserve your space for pre-scheduled instrument demonstration.
For more information and links to signup specific instrument demo, please visit: http://www.dental.umaryland.edu/core-imaging/open-house--instrument-demo/
We look forward to seeing you in Baltimore.
Sincerely, Ru-ching Hsia rhsia-at-umaryland.edu Director, Electron Microscopy Core Imaging Facility University of Maryland, Baltimore Tel: 410-706 7992 http://www.dental.umaryland.edu/Core-imaging Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
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Make your smart phone into a microscope using LEGO. M&M 2018 Baltimore
Bring your designs to the Outreach Booth at the Megabooth in the Exhibitors’ Hall asap. Elaine Ps if anyone has made instruments of SEM, TEM etc out of LEGO we would love to see them too.
Dr. Elaine C. Humphrey Advanced Microscopy Facility Bob Wright Science Centre A015 University of Victoria, Canada
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Email: mbrazil-at-vergason.com Name: Michael Brazil
Organization: Vergason Technology, Inc.
Title-Subject: [Filtered] Hitachi S-570 SEM small tools needed
Message: I have recently taken responsibility for a 34-year-old Hitachi S-570 SEM, tungsten filament. It did not come with filament alignment jig, height setting scale, or tools for changing apertures. If anyone has these rattling around, I'd be happy to purchase. If you need to keep them, I'd be very grateful for close-up pictures to allow me to replicate them. Hitachi may eventually offer to make new ones, but I suspect the cost will be prohibitive. Login Host: 162.216.232.2 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD
Organization: Kimberly-Clark
Title-Subject: [Filtered] Macroviewer/Light Box for Donation
Message: Macroviewer/Lightbox for donation Includes overhead lamps and movable stage Available for pickup in Wisconsin or shipping at donee's expense. Pictures available upon request
Login Host: 161.69.123.14 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD
Organization: Kimberly-Clark
Title-Subject: [Filtered] Inverted Microscope Stand for Donation
Message: Olympus IX70 inverted microscope stand for donation Great for customization Available for pickup in Wisconsin or can be shipped at donee's expense.
Pictures available upon request!
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I have recently taken over a Hitachi S-570 SEM, built in 1984. It lacks some of the small tools. I would like to purchase them. Hitachi does not stock. If you have some left lying around that you no longer need, great. If you have some that you need, would you be willing to send me photos with a scale in the image? It would help me recreate them. The particular items of interest are: Hitachi Part Number Description 531-1470 Filament Exchange Jig 538-0636 Scale (used for setting height) 538-1437 Tool for objective aperture set 538-1438 Tool for objective aperture exchange
Thank you, Michael Brazil Senior Scientist Vergason Technologies, Inc. 166 Route 224 Van Etten, NY 14889 (607) 589-3914 (office) mbrazil-at-vergason.com www.vergason.com www.linkedin.com/in/mbrazil/en
I would appreciate feedback from the group regarding your practices and policies of the calibration of your EDS systems.
A few topics of particular interest are:
How often do you perform the calibration routine? Are you satisfied that the automatic calibration is sufficient? Can you (and do you) collect "As found" as well as "As left" FWHM energy levels for any elements?
Thanks in advance for any and all thoughts!
Chris Holp FirstEnergy BETA Labs 6670 Beta Drive Mayfield Village OH 44143
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The early registration deadline for the NexTEM workshop at PNNL is today! Please read below for more details and visit https://pnnl.cvent.com/nextem to register. We have a stellar lineup of invited speakers, sessions, and social events and we hope you are able to attend.
----------------
Next-Generation Transmission Electron Microscopy (NexTEM) Workshop Beyond Current Limits of Resolution, Environments, and Data Analysis
Overview
Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.
Topics
High-Resolution Imaging and Spectroscopy – New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems. – Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision. – Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces. – Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples. – Novel detectors for improved high-speed imaging and spectroscopy.
In Situ / Operando Microscopy and Extreme Environments – Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids. – Investigation of materials under stimulus across a range of sample environments and temperatures.
Computational Methods for Data Analysis – Software to improve data collection quality, accuracy, and acquisition rates. – Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis. – Machine learning approaches for high-throughput data processing and feature detection. – Image simulation tools to aid the interpretation of experimental images and spectroscopy.
Confirmed Invited Speakers – Ondrej Krivanek, Nion Co. – Amanda Petford-Long, Argonne National Laboratory – Sergei Kalinin, Oak Ridge National Laboratory – Renu Sharma, National Institute of Standards and Technology – Rafal Dunin-Borkowski, Forschungszentrum Jülich – Robert Klie, University of Illinois–Chicago – Khalid Hattar, Sandia National Laboratories – Marc DeGraef, Carnegie Mellon University – James LeBeau, North Carolina State University – Haimei Zheng, Lawrence-Berkeley National Laboratory – Colin Ophus, Molecular Foundry – Juan Carlos Idrobo, Oak Ridge National Laboratory – Rama Vasudevan, Oak Ridge National Laboratory – R. Lee Penn, University of Minnesota – Paolo Longo, Gatan – Lewys Jones, Trinity College–Dublin
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
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Dear Listers, I'll be attending the M&M meeting in Baltimore. I would like to meet with anyone who is doing quantitative TEM using stereology.
Tom Bargar UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 402-559-7347
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Dear colleagues We are now planning the workshop “Atom by atom fabrication via electron beams and scanning probes”, to be held at Oak Ridge National Laboratory on November 1-2 this coming fall. This workshop will bring together experts in the emerging field of atomically resolved electron beam matter manipulation and scanning probe atomic fabrication, to highlight the recent advances and opportunities and serve as a much-needed seed to establish its rapid growth. It will provide a forum to present recent achievements in scanning probe and electron beam manipulation, novel opportunities for instrumental development enabled by the availability of high speed data analytic tools and machine learning, integration of atomic-scale device engineering into semiconductor workflows, and opportunities for quantum information systems and quantum computing. The confirmed plenary speakers at the workshop are Toma Susi (U Vienna) and J. Randall (Zyvex), with a number of invited talks by the leading experts in the field. Additional invited and contributed talks will be selected from submitted abstracts. If interested - mark the time in your calendars! Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, Foresight Institute, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
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We are delighted to provide information on two seminars run from Oak Ridge National Laboratory as part of the Center for Nanophase Materials Sciences User Meeting 2018 Workshops. Both workshops will focus on using python and available scientific packages to analyze a range of imaging and spectral datasets. The workshop details and timings are below:
1. Monday, August 13th 2018: Imaging and Spectral Data Analysis in Python
Full details are available on our github page,https://github.com/pycroscopy/pyUSID_Tutorial
Topics covered on Monday: Basic python, Jupyter notebooks, the pyUSID data model, basic spectral and image processing, rounding out with talks.
It will be streamed* on Bluejeans via the following link:https://bluejeans.com/782808739
1. Wednesday, August 13th 2018: Machine Learning for Materials Science with Python
Topics covered include Linear and Nonlinear Unmixing methods for spectral data, Basic classification (supervised and unsupervised), and an introduction to deep learning for microscopy datasets.
It will be streamed* via the following link: bluejeans.com/678595624 {http://bluejeans.com/678595624}
*Note that due to streaming bandwidth, there are a limited number of viewer slots. We apologize in advance if the link is non-functioning due to demand. But, both workshops will be recorded and posted at a later date.
Kind Regards, Rama Vasudevan on behalf of the organizing team Center for Nanophase Materials Sciences, Oak Ridge National Laboratory
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
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Title-Subject: [Filtered] EMS Microscopy Academy Workshops. Fall classes starting soon.
Message: Don't miss these exciting workshops coming this fall. Feel free to reach out to me with any questions. - Stacie Kirsch
Cryosectioning/Immunogold Workshop Led by Peter van de Plas, Helmut Gnaegi and Michael Kostrna Monday - Friday October 8 - 12, 2018 Hatfield, Pennsylvania, USA Five days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in cryosectioning and immunogold labeling Will provide researchers with the opportunity to learn the theory and practice of the use of cryosectioning with diamond knives, and immunogold labeling. Sign up at https://www.emsmicroscopyacademy.com/product-page/cryoimmuno
Cryo SEM Workshop Led by Al Coritz and Michael Kostrna Tuesday - Thursday October 23 - 25, 2018 Hatfield, Pennsylvania, USA This course will cover the process of rapid freezing, fracturing, coating and imaging of a variety of samples. For individuals who are new to the field of cryo SEM or desire a technical refresh to maintain current skills or just those that want to see and learn all of the possibilities of the technology. Sign up at https://www.emsmicroscopyacademy.com/product-page/cryosem
Biological TEM Workshop Led by Al Coritz and Michael Kostrna Tuesday - Thursday November 6 - 8, 2018 Hatfield, Pennsylvania, USA Theory and practical preparation of buffers, fixatives, and other solutions required for chemical processing of biological samples for TEM. For individuals who are, or will be, responsible for chemical processing and sectioning of biological samples for TEM examination. Sign up at https://www.emsmicroscopyacademy.com/product-page/biotem
Biological SEM Workshop Led by Al Coritz and Michael Kostrna Tuesday - Thursday November 13 - 15, 2018 Hatfield, Pennsylvania, USA This course will introduce participants to methods of sample preparation and SEM parameters and operation needed for accurate analysis. For individuals who currently perform or will be responsible for the preparation of samples and/or operation of the SEM in a research, academic, or industrial setting. Sign up at https://www.emsmicroscopyacademy.com/product-page/biosem
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop again!
Message: October 2018 This intensive, three-day workshop provides a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and hands-on training led by GEM staff. What: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Wednesday through Friday, October 24-26, 2018, 8am-5pm each day (lunch is provided) Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login Deadline: Oct 17, 2018
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Thank you for interest in the Machine learning for materials science with python workshop, part of the CNMS User Meeting 2018. The bluejeans link is https://ornl.bluejeans.com/1766282540
You may find the agenda and course materials available on github here:
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
The George Washington University Nanofabrication and Imaging Center would like to announce it's fall workshop.
Please join us Sept 24-28, 2018 in Washington DC for a week of intensive CLEM presentations, demonstrations and discussions. Please follow the link below for more information.
https://nic.gwu.edu/clem-workshop-2018
Chris Brantner GWNIC staff gwnic-at-gwu.edu
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Has anyone ever tried (or seen literature on) EBSD or transmission Kikuchi diffraction of organic crystals? Ever has any success? I would be interested in any experiences you could share.
Vendors, please contact me off list about your highest-sensitivity EBSD and especially tKD systems.
Thanks Chad
-------------------------------- Chad M. Parish, Ph.D. Research and Development Staff Member Radiation Effects and Microstructural Analysis Team Nuclear Materials Science and Technology Group Materials Science and Technology Division Oak Ridge National Laboratory Phone: 1 865 574 0092 Email: parishcm-at-ornl.gov
==============================Original Headers============================== 6, 50 -- From parishcm-at-ornl.gov Thu Aug 16 11:57:29 2018 6, 50 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.137]) 6, 50 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w7GGvTKi009991 6, 50 -- for {Microscopy-at-microscopy.com} ; Thu, 16 Aug 2018 11:57:29 -0500 6, 50 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 50 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 6, 50 -- t=1534438552; x=1565974552; 6, 50 -- h=from:to:subject:date:message-id: 6, 50 -- content-transfer-encoding:mime-version; 6, 50 -- bh=5wzqyMj6d6lZbrvCw+Wd2GjTtH4okus7EMAyvxeNBfQ=; 6, 50 -- b=wl7Ewj0+VzeIdBaa6KtvQvKdJIo7KmCga094YeT9XmNodQKEoUgCvpco 6, 50 -- skHVpbyNfYHXNJZqw71yfSApuH2vuMBHNyNi2c6lOLo3ApR82Pm3r/TD5 6, 50 -- CMhSK4u2ekoOqrQs6xUHqZGK646lRgaHBtKpGiu6JmwYWreXhpH0KOhAj 6, 50 -- L4AWyhMSu9rSpEhXXCPmLKlNeuWwEir6EfrhzzfVx1zxpBvMY1JNfumsr 6, 50 -- Mr+fQPsMnn61K3kb2sClG7iLSpdmKaifHTrQkrMzWS5ZhIMWmIjmv1u4P 6, 50 -- 9Gk3MESUFJ04vTi9mianlMdyvJunBGUAQXL3tEOgUXPsaDNGayqHDrkO7 6, 50 -- g==; 6, 50 -- X-SG: RELAYLIST 6, 50 -- X-IronPort-AV: E=Sophos;i="5.53,247,1531800000"; 6, 50 -- d="scan'208";a="45718423" 6, 50 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 6, 50 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 16 Aug 2018 12:55:52 -0400 6, 50 -- Received: from EXCHOS32.ornl.gov (exchos32.ornl.gov [128.219.12.152]) 6, 50 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 6, 50 -- (No client certificate requested) 6, 50 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 41rssh31y9z2SswR 6, 50 -- for {Microscopy-at-Microscopy.Com} ; Thu, 16 Aug 2018 12:55:52 -0400 (EDT) 6, 50 -- Received: from EXCHOS32.ornl.gov (128.219.12.152) by EXCHOS32.ornl.gov 6, 50 -- (128.219.12.152) with Microsoft SMTP Server (TLS) id 15.0.1367.3; Thu, 16 Aug 6, 50 -- 2018 12:55:52 -0400 6, 50 -- Received: from EXCHOS32.ornl.gov ([fe80::4d64:9baf:7ffb:31ca]) by 6, 50 -- EXCHOS32.ornl.gov ([fe80::4d64:9baf:7ffb:31ca%18]) with mapi id 6, 50 -- 15.00.1367.000; Thu, 16 Aug 2018 12:55:52 -0400 6, 50 -- From: "Parish, Chad M." {parishcm-at-ornl.gov} 6, 50 -- To: "'Microscopy-at-Microscopy.Com'" {Microscopy-at-microscopy.com} 6, 50 -- Subject: EBSD of organics 6, 50 -- Thread-Topic: EBSD of organics 6, 50 -- Thread-Index: AdQ1gfzhWA5BPvyvT9qr5mkTu02KQg== 6, 50 -- Date: Thu, 16 Aug 2018 16:55:52 +0000 6, 50 -- Message-ID: {e75b4ef29f7140af9bab6e485adbe4e5-at-EXCHOS32.ornl.gov} 6, 50 -- Accept-Language: en-US 6, 50 -- Content-Language: en-US 6, 50 -- X-MS-Has-Attach: 6, 50 -- X-MS-TNEF-Correlator: 6, 50 -- x-ms-exchange-transport-fromentityheader: Hosted 6, 50 -- x-originating-ip: [128.219.12.135] 6, 50 -- Content-Type: text/plain; charset="us-ascii" 6, 50 -- MIME-Version: 1.0 6, 50 -- Content-Transfer-Encoding: 8bit 6, 50 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w7GGvTKi009991 ==============================End of - Headers==============================
Long shot... we have an abrasion saw - a Struers Secotom 50 - and somebody has lost the little plastic lid that goes on the hole you use for emptying out the cooling water tank, so we can't fill the tank up and run the saw. It is a little round plastic lid, 20mm in diameter, like a bottle top.
Does anybody out there maybe have a dead Struers Secotom 50 that has that lid still? Or a Struers anything with a lid like it, in case they used the same one?
We have looked *everywhere*. We have asked if they will sell us a lid and the answer was "it's complicated". I am measuring every bottle lid I see just in case it fits...
Sorry this is not entirely a microscopy question, but it is delaying a lot of microscopy....
It’s not complicated: Struers customer service is…missing in action. Are they going out of business?
} On Aug 20, 2018, at 9:25 AM, j.sharp-at-sheffield.ac.uk wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=t3ts5kvraXgyYnidcv_L0MFCz1QOhT_c4f3-030WL7s&s=KqFizYgNjQx5eK5wEWz4oZRvLT2Lk3f7WCW5N6zr--s&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=t3ts5kvraXgyYnidcv_L0MFCz1QOhT_c4f3-030WL7s&s=CkfM9GkIOKhfL8hf3qjAFLY3cU4GVI3GkgMhv_u2rvo&e= } ---------------------------------------------------------------------------- } } Hi everybody, } } Long shot... we have an abrasion saw - a Struers Secotom 50 - and } somebody has lost the little plastic lid that goes on the hole you use } for emptying out the cooling water tank, so we can't fill the tank up } and run the saw. It is a little round plastic lid, 20mm in diameter, } like a bottle top. } } Does anybody out there maybe have a dead Struers Secotom 50 that has } that lid still? Or a Struers anything with a lid like it, in case they } used the same one? } } We have looked *everywhere*. We have asked if they will sell us a lid } and the answer was "it's complicated". I am measuring every bottle lid } I see just in case it fits... } } Sorry this is not entirely a microscopy question, but it is delaying a } lot of microscopy.... } } Yours hopefully } } Jo Sharp, postdoc, Sheffield } } ==============================Original Headers============================== } 7, 42 -- From j.sharp-at-sheffield.ac.uk Mon Aug 20 11:19:46 2018 } 7, 42 -- Received: from mail-oi0-f43.google.com (mail-oi0-f43.google.com [209.85.218.43]) } 7, 42 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w7KGJiGt028696 } 7, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2018 11:19:45 -0500 } 7, 42 -- Received: by mail-oi0-f43.google.com with SMTP id w126-v6so26793307oie.7 } 7, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2018 09:18:21 -0700 (PDT) } 7, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 42 -- d=sheffield.ac.uk; s=170424.google; } 7, 42 -- h=mime-version:from:date:message-id:subject:to; } 7, 42 -- bh=Xzv5A1bTSDZLZgu5nemcad46EmcnsVbh789HV0aYGao=; } 7, 42 -- b=Zn46K1Ff4GCfp9ay9aqELtqdUQH38Ly4Cu5MGuxvrhAzVXa2kOmDhKGnKPVo7k+aEQ } 7, 42 -- cQU8ypvPWTqUbPoS2CIehsSu6I4zcdRNcbIaEJKx6W1v2IkZnGbRstJAj8g0RBIcBGlv } 7, 42 -- RvNBBAns4d6ouK10r3wFJIdjM1J/7ddUieTCsWPZzasglfCSXhewiYURPe311y823k7H } 7, 42 -- LXVzhcDzVWLlS0A97s8yOpsVtfXNlZabkiBl7yVW5cwSZe0ZfsqXwDZ2sB9zMf1sBtJ7 } 7, 42 -- VW4qM6ejGh1l9vsIhxlr/BHYpQn8ea0xaGKTa3WLYjvgKwdCFZC4QuLNT59LnA4xqR4r } 7, 42 -- jPYQ== } 7, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 42 -- d=1e100.net; s=20161025; } 7, 42 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 7, 42 -- bh=Xzv5A1bTSDZLZgu5nemcad46EmcnsVbh789HV0aYGao=; } 7, 42 -- b=avjMYrzKhjASV0xoFfT0VZC0IPx4PSrxXKjD+2/P4CLPmuJet/VoXGk8mwADxgHjMo } 7, 42 -- U8orB1x52TOqYarXeGkJSEU/Hw0tZ+JdQPn+/u+F8O6TtIvLfZrwjGJkiTvDqCPZDzPn } 7, 42 -- k2xK49VxHJmmCss3fV8Sovw8ngIPSEpo0yClhgeVp3KumS0uyiiFjsNxWzblcwfdx9uX } 7, 42 -- 23sR831lCwR3BNLvdNhbEK+l/Wdux1w2VzvVp99Z76JhM9xQW0FZ0NJ4+LEBN2h0WGTN } 7, 42 -- 0gFSasz3WGUaExpYGcJC3Dn7wEinaHVcMTluXNdNEIT4EVHbzmKNif7tfWpiWENYjl3L } 7, 42 -- q1aw== } 7, 42 -- X-Gm-Message-State: AOUpUlEESSylI2PcvIQ2m7Z0zQiGrPr2Nhxe0Ge1NtYA4WJnoIPCNz/8 } 7, 42 -- Q8mknac/HQ0GsZpNI9NFU5z0I5ZV2phIaOhNILD9kHm92Z+BfklQPQ6eWWqyHHi8D6Ryr6RIBfL } 7, 42 -- F8/g9OCYfISJlEoR4NP9bWS870biQkfZDqXGA8zJhGCV9q7sZSXS4vE1plOvHW5LMVsbbUZJsQc } 7, 42 -- huWruOi+yO3ASnKNQ7auArbuN1OrTBqPehyEl6 } 7, 42 -- X-Google-Smtp-Source: AA+uWPy81GpEzc6KC0ffMwZig/4O/05pNczxOYUn9tbcxT5FReJfAoVMI589cdKqABXmytHDGpUW8zFO/zm2nfkV27c= } 7, 42 -- X-Received: by 2002:aca:5003:: with SMTP id e3-v6mr15012864oib.89.1534774723806; } 7, 42 -- Mon, 20 Aug 2018 07:18:43 -0700 (PDT) } 7, 42 -- MIME-Version: 1.0 } 7, 42 -- Received: by 2002:a4a:7242:0:0:0:0:0 with HTTP; Mon, 20 Aug 2018 07:18:03 } 7, 42 -- -0700 (PDT) } 7, 42 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk} } 7, 42 -- Date: Mon, 20 Aug 2018 15:18:03 +0100 } 7, 42 -- Message-ID: {CAG8d=kVVJ3XMFd_zJJDeRZ9s-LBtfg5e5uYdKqQGzTrJbCnUgQ-at-mail.gmail.com} } 7, 42 -- Subject: Struers secotom? } 7, 42 -- To: Microscopy-at-microscopy.com } 7, 42 -- Content-Type: text/plain; charset="UTF-8" } ==============================End of - Headers==============================
Well they came to fix our Tenupol a few weeks ago, so they're not completely missing, they just can't instantly send us this one thing.
Jo
On 20 August 2018 at 18:30, John Mardinly {John.Mardinly-at-asu.edu} wrote: } It’s not complicated: Struers customer service is…missing in action. Are they going out of business? } } } On Aug 20, 2018, at 9:25 AM, j.sharp-at-sheffield.ac.uk wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=t3ts5kvraXgyYnidcv_L0MFCz1QOhT_c4f3-030WL7s&s=KqFizYgNjQx5eK5wEWz4oZRvLT2Lk3f7WCW5N6zr--s&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=t3ts5kvraXgyYnidcv_L0MFCz1QOhT_c4f3-030WL7s&s=CkfM9GkIOKhfL8hf3qjAFLY3cU4GVI3GkgMhv_u2rvo&e= } } ---------------------------------------------------------------------------- } } } } Hi everybody, } } } } Long shot... we have an abrasion saw - a Struers Secotom 50 - and } } somebody has lost the little plastic lid that goes on the hole you use } } for emptying out the cooling water tank, so we can't fill the tank up } } and run the saw. It is a little round plastic lid, 20mm in diameter, } } like a bottle top. } } } } Does anybody out there maybe have a dead Struers Secotom 50 that has } } that lid still? Or a Struers anything with a lid like it, in case they } } used the same one? } } } } We have looked *everywhere*. We have asked if they will sell us a lid } } and the answer was "it's complicated". I am measuring every bottle lid } } I see just in case it fits... } } } } Sorry this is not entirely a microscopy question, but it is delaying a } } lot of microscopy.... } } } } Yours hopefully } } } } Jo Sharp, postdoc, Sheffield } } } } ==============================Original Headers============================== } } 7, 42 -- From j.sharp-at-sheffield.ac.uk Mon Aug 20 11:19:46 2018 } } 7, 42 -- Received: from mail-oi0-f43.google.com (mail-oi0-f43.google.com [209.85.218.43]) } } 7, 42 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w7KGJiGt028696 } } 7, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2018 11:19:45 -0500 } } 7, 42 -- Received: by mail-oi0-f43.google.com with SMTP id w126-v6so26793307oie.7 } } 7, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2018 09:18:21 -0700 (PDT) } } 7, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 7, 42 -- d=sheffield.ac.uk; s=170424.google; } } 7, 42 -- h=mime-version:from:date:message-id:subject:to; } } 7, 42 -- bh=Xzv5A1bTSDZLZgu5nemcad46EmcnsVbh789HV0aYGao=; } } 7, 42 -- b=Zn46K1Ff4GCfp9ay9aqELtqdUQH38Ly4Cu5MGuxvrhAzVXa2kOmDhKGnKPVo7k+aEQ } } 7, 42 -- cQU8ypvPWTqUbPoS2CIehsSu6I4zcdRNcbIaEJKx6W1v2IkZnGbRstJAj8g0RBIcBGlv } } 7, 42 -- RvNBBAns4d6ouK10r3wFJIdjM1J/7ddUieTCsWPZzasglfCSXhewiYURPe311y823k7H } } 7, 42 -- LXVzhcDzVWLlS0A97s8yOpsVtfXNlZabkiBl7yVW5cwSZe0ZfsqXwDZ2sB9zMf1sBtJ7 } } 7, 42 -- VW4qM6ejGh1l9vsIhxlr/BHYpQn8ea0xaGKTa3WLYjvgKwdCFZC4QuLNT59LnA4xqR4r } } 7, 42 -- jPYQ== } } 7, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 7, 42 -- d=1e100.net; s=20161025; } } 7, 42 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } } 7, 42 -- bh=Xzv5A1bTSDZLZgu5nemcad46EmcnsVbh789HV0aYGao=; } } 7, 42 -- b=avjMYrzKhjASV0xoFfT0VZC0IPx4PSrxXKjD+2/P4CLPmuJet/VoXGk8mwADxgHjMo } } 7, 42 -- U8orB1x52TOqYarXeGkJSEU/Hw0tZ+JdQPn+/u+F8O6TtIvLfZrwjGJkiTvDqCPZDzPn } } 7, 42 -- k2xK49VxHJmmCss3fV8Sovw8ngIPSEpo0yClhgeVp3KumS0uyiiFjsNxWzblcwfdx9uX } } 7, 42 -- 23sR831lCwR3BNLvdNhbEK+l/Wdux1w2VzvVp99Z76JhM9xQW0FZ0NJ4+LEBN2h0WGTN } } 7, 42 -- 0gFSasz3WGUaExpYGcJC3Dn7wEinaHVcMTluXNdNEIT4EVHbzmKNif7tfWpiWENYjl3L } } 7, 42 -- q1aw== } } 7, 42 -- X-Gm-Message-State: AOUpUlEESSylI2PcvIQ2m7Z0zQiGrPr2Nhxe0Ge1NtYA4WJnoIPCNz/8 } } 7, 42 -- Q8mknac/HQ0GsZpNI9NFU5z0I5ZV2phIaOhNILD9kHm92Z+BfklQPQ6eWWqyHHi8D6Ryr6RIBfL } } 7, 42 -- F8/g9OCYfISJlEoR4NP9bWS870biQkfZDqXGA8zJhGCV9q7sZSXS4vE1plOvHW5LMVsbbUZJsQc } } 7, 42 -- huWruOi+yO3ASnKNQ7auArbuN1OrTBqPehyEl6 } } 7, 42 -- X-Google-Smtp-Source: AA+uWPy81GpEzc6KC0ffMwZig/4O/05pNczxOYUn9tbcxT5FReJfAoVMI589cdKqABXmytHDGpUW8zFO/zm2nfkV27c= } } 7, 42 -- X-Received: by 2002:aca:5003:: with SMTP id e3-v6mr15012864oib.89.1534774723806; } } 7, 42 -- Mon, 20 Aug 2018 07:18:43 -0700 (PDT) } } 7, 42 -- MIME-Version: 1.0 } } 7, 42 -- Received: by 2002:a4a:7242:0:0:0:0:0 with HTTP; Mon, 20 Aug 2018 07:18:03 } } 7, 42 -- -0700 (PDT) } } 7, 42 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk} } } 7, 42 -- Date: Mon, 20 Aug 2018 15:18:03 +0100 } } 7, 42 -- Message-ID: {CAG8d=kVVJ3XMFd_zJJDeRZ9s-LBtfg5e5uYdKqQGzTrJbCnUgQ-at-mail.gmail.com} } } 7, 42 -- Subject: Struers secotom? } } 7, 42 -- To: Microscopy-at-microscopy.com } } 7, 42 -- Content-Type: text/plain; charset="UTF-8" } } ==============================End of - Headers============================== }
Jo, maybe you could replace it (at least temporarily, until they will manage to send you the part) by something hand-made from soft rubber or cork? 20 mm in diameter, it looks like wine or champagne cork :-). Good luck, Tomas ________________________________________ X-from: j.sharp-at-sheffield.ac.uk {j.sharp-at-sheffield.ac.uk} Sent: 21 August 2018 12:08 To: Tom Hrn
Well they came to fix our Tenupol a few weeks ago, so they're not completely missing, they just can't instantly send us this one thing.
Jo
On 20 August 2018 at 18:30, John Mardinly {John.Mardinly-at-asu.edu} wrote: } Its not complicated: Struers customer service ismissing in action. Are they going out of business? } } } On Aug 20, 2018, at 9:25 AM, j.sharp-at-sheffield.ac.uk wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=t3ts5kvraXgyYnidcv_L0MFCz1QOhT_c4f3-030WL7s&s=KqFizYgNjQx5eK5wEWz4oZRvLT2Lk3f7WCW5N6zr--s&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=t3ts5kvraXgyYnidcv_L0MFCz1QOhT_c4f3-030WL7s&s=CkfM9GkIOKhfL8hf3qjAFLY3cU4GVI3GkgMhv_u2rvo&e= } } ---------------------------------------------------------------------------- } } } } Hi everybody, } } } } Long shot... we have an abrasion saw - a Struers Secotom 50 - and } } somebody has lost the little plastic lid that goes on the hole you use } } for emptying out the cooling water tank, so we can't fill the tank up } } and run the saw. It is a little round plastic lid, 20mm in diameter, } } like a bottle top. } } } } Does anybody out there maybe have a dead Struers Secotom 50 that has } } that lid still? Or a Struers anything with a lid like it, in case they } } used the same one? } } } } We have looked *everywhere*. We have asked if they will sell us a lid } } and the answer was "it's complicated". I am measuring every bottle lid } } I see just in case it fits... } } } } Sorry this is not entirely a microscopy question, but it is delaying a } } lot of microscopy.... } } } } Yours hopefully } } } } Jo Sharp, postdoc, Sheffield } } } } ==============================Original Headers============================== } } 7, 42 -- From j.sharp-at-sheffield.ac.uk Mon Aug 20 11:19:46 2018 } } 7, 42 -- Received: from mail-oi0-f43.google.com (mail-oi0-f43.google.com [209.85.218.43]) } } 7, 42 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w7KGJiGt028696 } } 7, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2018 11:19:45 -0500 } } 7, 42 -- Received: by mail-oi0-f43.google.com with SMTP id w126-v6so26793307oie.7 } } 7, 42 -- for {Microscopy-at-microscopy.com} ; Mon, 20 Aug 2018 09:18:21 -0700 (PDT) } } 7, 42 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 7, 42 -- d=sheffield.ac.uk; s=170424.google; } } 7, 42 -- h=mime-version:from:date:message-id:subject:to; } } 7, 42 -- bh=Xzv5A1bTSDZLZgu5nemcad46EmcnsVbh789HV0aYGao=; } } 7, 42 -- b=Zn46K1Ff4GCfp9ay9aqELtqdUQH38Ly4Cu5MGuxvrhAzVXa2kOmDhKGnKPVo7k+aEQ } } 7, 42 -- cQU8ypvPWTqUbPoS2CIehsSu6I4zcdRNcbIaEJKx6W1v2IkZnGbRstJAj8g0RBIcBGlv } } 7, 42 -- RvNBBAns4d6ouK10r3wFJIdjM1J/7ddUieTCsWPZzasglfCSXhewiYURPe311y823k7H } } 7, 42 -- LXVzhcDzVWLlS0A97s8yOpsVtfXNlZabkiBl7yVW5cwSZe0ZfsqXwDZ2sB9zMf1sBtJ7 } } 7, 42 -- VW4qM6ejGh1l9vsIhxlr/BHYpQn8ea0xaGKTa3WLYjvgKwdCFZC4QuLNT59LnA4xqR4r } } 7, 42 -- jPYQ== } } 7, 42 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 7, 42 -- d=1e100.net; s=20161025; } } 7, 42 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } } 7, 42 -- bh=Xzv5A1bTSDZLZgu5nemcad46EmcnsVbh789HV0aYGao=; } } 7, 42 -- b=avjMYrzKhjASV0xoFfT0VZC0IPx4PSrxXKjD+2/P4CLPmuJet/VoXGk8mwADxgHjMo } } 7, 42 -- U8orB1x52TOqYarXeGkJSEU/Hw0tZ+JdQPn+/u+F8O6TtIvLfZrwjGJkiTvDqCPZDzPn } } 7, 42 -- k2xK49VxHJmmCss3fV8Sovw8ngIPSEpo0yClhgeVp3KumS0uyiiFjsNxWzblcwfdx9uX } } 7, 42 -- 23sR831lCwR3BNLvdNhbEK+l/Wdux1w2VzvVp99Z76JhM9xQW0FZ0NJ4+LEBN2h0WGTN } } 7, 42 -- 0gFSasz3WGUaExpYGcJC3Dn7wEinaHVcMTluXNdNEIT4EVHbzmKNif7tfWpiWENYjl3L } } 7, 42 -- q1aw== } } 7, 42 -- X-Gm-Message-State: AOUpUlEESSylI2PcvIQ2m7Z0zQiGrPr2Nhxe0Ge1NtYA4WJnoIPCNz/8 } } 7, 42 -- Q8mknac/HQ0GsZpNI9NFU5z0I5ZV2phIaOhNILD9kHm92Z+BfklQPQ6eWWqyHHi8D6Ryr6RIBfL } } 7, 42 -- F8/g9OCYfISJlEoR4NP9bWS870biQkfZDqXGA8zJhGCV9q7sZSXS4vE1plOvHW5LMVsbbUZJsQc } } 7, 42 -- huWruOi+yO3ASnKNQ7auArbuN1OrTBqPehyEl6 } } 7, 42 -- X-Google-Smtp-Source: AA+uWPy81GpEzc6KC0ffMwZig/4O/05pNczxOYUn9tbcxT5FReJfAoVMI589cdKqABXmytHDGpUW8zFO/zm2nfkV27c= } } 7, 42 -- X-Received: by 2002:aca:5003:: with SMTP id e3-v6mr15012864oib.89.1534774723806; } } 7, 42 -- Mon, 20 Aug 2018 07:18:43 -0700 (PDT) } } 7, 42 -- MIME-Version: 1.0 } } 7, 42 -- Received: by 2002:a4a:7242:0:0:0:0:0 with HTTP; Mon, 20 Aug 2018 07:18:03 } } 7, 42 -- -0700 (PDT) } } 7, 42 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk} } } 7, 42 -- Date: Mon, 20 Aug 2018 15:18:03 +0100 } } 7, 42 -- Message-ID: {CAG8d=kVVJ3XMFd_zJJDeRZ9s-LBtfg5e5uYdKqQGzTrJbCnUgQ-at-mail.gmail.com} } } 7, 42 -- Subject: Struers secotom? } } 7, 42 -- To: Microscopy-at-microscopy.com } } 7, 42 -- Content-Type: text/plain; charset="UTF-8" } } ==============================End of - Headers============================== }
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Organization: Centers for Disease Control and Prevention (CDC)
Title-Subject: [Filtered] Seeking a life sciences electron microscopist
Message: The Infectious Diseases Pathology Branch, National Center for Emerging and Zoonotic Diseases, Centers for Disease Control and Prevention (CDC) is seeking a person with transmission electron microscopy (TEM) experience. Our laboratory uses thin section and negative stain techniques to diagnose and characterize pathogens of public health importance, including viral, bacterial, and parasitic diseases. The EM lab has been instrumental during outbreaks of SARS coronavirus, poxviruses, Zika virus, Nipah virus, transplant-associated viral transmissions, and the hemorrhagic fever viruses. Projects have included the EM description of emerging viruses, characterization of humanized mice infected with BSL-4 viruses, quality control of virus-like particles to be used in immunoassays, and examination by TEM of formalin-fixed paraffin-embedded specimens that have been submitted to the branch. CDC serves as the federal government agency for public health concerns, and is a reference center for US state health departments and for the World Health Organization. The Infectious Diseases Pathology Branch consists of pathologists, histotechnologists, molecular biologists, a microbiologist, and an electron microscopist who work together to obtain a diagnosis by using tissues and tissue culture isolates. To further the characterization of infectious diseases, the EM lab also uses immunogold labeling and in situ hybridization techniques. Equipment available includes two FEI/Thermo Fisher 120 kV transmission electron microscopes each with an AMT BioSprint 16 camera, a Pelco BioWave Pro+ microwave for tissue processing, and two Leica ultramicrotomes (UC7 and UC UCT).
The ideal candidate would have a minimum of a Bachelor's degree in biology or related discipline and some experience with biological TEM techniques, including thin section and negative stain methods. Capability with TEM instrument usage plus knowledge of microbiology and/or molecular biology would be advantageous. The applicant should be a US citizen or a permanent resident. Compensation will be commensurate with education and experience. CDC is an equal opportunity employer, and is a smoke-free environment.
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Email: yuziliu-at-anl.gov Name: Yuzi Liu
Organization: Argonne National Laboratory
Title-Subject: [Filtered] Postdoc position available
Message: We are seeking a self-motivated and creative postdoctoral fellow to conduct experimental research in the areas of cathode materials for sodium-ion batteries. In this role, you will use your experience and education in the fields related to solid state synthesis of electrode materials, electrochemistry, and advanced characterization and data analysis (e.g.,analytical TEM, XRD, Rieltveld refinement, synchrotron spectroscopy) to support ongoing efforts and to develop and lead new efforts. This project is a close collaboration between the Electrochemical Energy Materials Lab at Boise State University (clairexiong-at-boisestate.edu) and Argonne National Lab. Travels to Argonne annually are expected for this position. Hands on experience and data analysis on (S)TEM are highly desired. Thank you!
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This is a final reminder that registration for the NexTEM workshop at PNNL closes this Friday, August 31st. We have over 40 registrants already, including distinguished speakers from around the world, and we hope you'll be able to join us. Please read below for more details and visit https://pnnl.cvent.com/nextem to sign up today!
----------------
Next-Generation Transmission Electron Microscopy (NexTEM) Workshop Beyond Current Limits of Resolution, Environments, and Data Analysis
Overview
Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.
Topics
High-Resolution Imaging and Spectroscopy – New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems. – Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision. – Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces. – Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples. – Novel detectors for improved high-speed imaging and spectroscopy.
In Situ / Operando Microscopy and Extreme Environments – Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids. – Investigation of materials under stimulus across a range of sample environments and temperatures.
Computational Methods for Data Analysis – Software to improve data collection quality, accuracy, and acquisition rates. – Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis. – Machine learning approaches for high-throughput data processing and feature detection. – Image simulation tools to aid the interpretation of experimental images and spectroscopy.
Confirmed Invited Speakers – Ondrej Krivanek, Nion Co. – Amanda Petford-Long, Argonne National Laboratory – Sergei Kalinin, Oak Ridge National Laboratory – Renu Sharma, National Institute of Standards and Technology – Rafal Dunin-Borkowski, Forschungszentrum Jülich – Robert Klie, University of Illinois–Chicago – Khalid Hattar, Sandia National Laboratories – Marc DeGraef, Carnegie Mellon University – James LeBeau, North Carolina State University – Haimei Zheng, Lawrence-Berkeley National Laboratory – Colin Ophus, Molecular Foundry – Juan Carlos Idrobo, Oak Ridge National Laboratory – Rama Vasudevan, Oak Ridge National Laboratory – R. Lee Penn, University of Minnesota – Paolo Longo, Gatan – Lewys Jones, Trinity College–Dublin
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
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Dear Listers,
I would like to get in contact with any experienced users of the Leica High Pressure Freezer and Freeze Substitution instruments. Please respond privately with your contact information as I would like to call and explain what I am experiencing. Easier to explain directly than to try and write it out.
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Dear colleagues If you are interested in applying STEM not only to visualize matter, but also to control it, move atom, and build devices by electron beam - the registration is now open for the inaugural workshop “Atom by atom fabrication via electron beams and scanning probes”, to be held at Oak Ridge National Laboratory on November 1,2. This workshop will bring together experts in scanning probe atomic fabrication and the rapidly emerging field of atomically resolved electron beam matter manipulation, to highlight the recent advances and opportunities and serve as a much-needed seed to establish its rapid growth. It will provide a forum to present recent achievements in electron beam manipulation, novel opportunities for instrumental development enabled by the availability of high speed data analytic tools and machine learning, integration of atomic-scale device engineering into semiconductor workflows, an opportunities for quantum information systems. The confirmed plenary speakers include Toma Susi (U. Vienna) and John Randall (Zyvex), and invited speakers (partial and tentative list) include Joe Lyding (Illinois), David Forrest (DOE), Dirk Englund (MIT), Prineha Narang (Harvard), Quentin Ramasse (SuperSTEM), Marija Drndic (U Penn), A. Lupini (ORNL).
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both lefty-at-gene.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: lefty-at-gene.com Name: Dave Lawrence
Organization: Genentech
Title-Subject: [Filtered] Reichert CS auto Freeze unit
Message: Hello, does anyone have any info on a Type 702101 sn 400660 Reichert auto Freeze unit? It's kind of very old and we have a lab that wants to use this for some experiments.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both jhyun-at-gatan.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School October 2018
Message: EELS & EFTEM Analysis Training School October 2018
Location: Gatan R&D Headquarters, Pleasanton, CA
Dear Colleagues,
I am looking for a postdoc in analytical STEM and APT of quantum materials systems. If you or anyone you know is interested, please see the details below and apply at: https://pnnl.jibeapply.com/jobs/308260?lang=en-us
--------------------------------------------------------------------------------- Post Doc Research Associate - Quantum Materials Microscopy and Tomography Locations: RICHLAND, WA Job ID: 308260 Full/Part Time: Full-Time Regular/Temporary: Temporary
Job Description The successful candidate will utilize aberration-corrected scanning transmission electron microscopy (STEM) and atom probe tomography (APT) to examine III/V semiconductors for quantum materials systems. Approximately 50% of the candidate’s time will be spent preparing TEM samples, conducting atomic-resolution imaging and chemical analysis using STEM, energy-dispersive X-ray spectroscopy (EDS), and electron energy loss spectroscopy (EELS). The other 50% of the candidate’s time will be spent preparing APT specimens, APT experimental data collection, reconstruction, and detailed analysis, with the goal of generating complementary datasets. The candidate will be encouraged to propose, plan, and conduct their own related research. The candidate is also expected to have expert level proficiency in focused ion beam (FIB)-based site specific lift-out preparation of TEM and APT samples.
Equal Employment Opportunity Battelle Memorial Institute (BMI) at Pacific Northwest National Laboratory (PNNL) is an Affirmative Action/Equal Opportunity Employer and supports diversity in the workplace. All employment decisions are made without regard to race, color, religion, sex, national origin, age, disability, veteran status, marital or family status, sexual orientation, gender identity, or genetic information. All BMI staff must be able to demonstrate the legal right to work in the United States. BMI is an E-Verify employer. Learn more at jobs.pnnl.gov.
Minimum Qualifications Candidates must have received a PhD within the past five years (60 months) or within the next 8 months from an accredited college or university.
Preferred Qualifications • At least 3 years of experience in Scanning / Transmission Electron Microscopy. • Strong oral and written communication skills. • Ph.D. in Materials Science and/or Engineering or Physics. • Candidates must have a strong background in their major field of study, the ability to work independently with little supervision, and an interest in working with others as part of an interdisciplinary team of researchers addressing challenges with national and international impact. • Experience in TEM and/or APT sample preparation using FIB and/or mechanical polishing techniques. • Experience in conducting atomic-resolution STEM imaging, EDS, and EELS spectroscopy of complex oxides or semiconductors. • Experience in laser assisted and voltage APT experimental procedures, reconstruction of data using IVAS software and detailed APT data analysis. • Experience with direct correlation of APT and STEM. • Specific expertise on APT analysis of semiconductor devices and understanding of strategies to correct reconstruction artifacts and increase analysis yield. • Familiarity with image simulation (multislice / Bloch wave) techniques for the interpretation of STEM images and spectroscopic data. • Familiarity with image analysis tools (ImageJ, Matlab) and Python programming skills.
______________________________________ Steven R. Spurgeon, Ph.D. Staff Scientist Energy and Environment Directorate
Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999 MSIN:P7-25 Richland, WA 99352
Currently, Andy Lupini and I have several postdoctoral positions open in the area of high resolution STEM-EELS of quantum materials. The perspective candidate will have access to the full spectrum of STEM tools at ORNL (including Nion MAC-STEM, U200, etc), and will have an opportunity to collaborate with broad range of theorists and materials synthesis groups at ORNL and worldwide, and also will have an opportunity to work with big data/machine learning groups at ORNL. Please contact me and Andy directly (sergei2-at-ornl.gov and arl1000-at-ornl.gov).
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
The George Washington University Nanofabrication and Imaging Center would like to announce it's fall workshop.
Please join us Sept 24-28, 2018 in Washington DC for a week of intensive CLEM presentations, demonstrations and discussions. Please follow the link below for more information.
https://nic.gwu.edu/clem-workshop-2018
Chris Brantner GWNIC staff gwnic-at-gwu.edu
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I am quite sure that I read a discussion about this before but I cannot find it anymore so I'm asking again, sorry for that. We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. The magnification given by the microscope corresponds to the image on the phosphor screen. But since the camera is not in the same plane and the beam is not parallel, the magnification on the camera is different.
Of course I calibrate the camera so my measurements on pictures taken by the camera are (more or less) right, but how can I know exactly at which magnification the picture has been taken? And, even more importantly, does it matter? Since the digital pictures can be printed at (almost) any size, this would mean that I end up with different magnifications, am I right?
If the microscope tells me that I used a 40 000x magnification, what should I write in my digital picture if I want to write a magnification additionally to the scale bar? Or does the question make any sense at all?
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Email: plappleton-at-dundee.ac.uk Name: Paul Appleton
Message: A great opportunity now available in the Dundee Imaging Facility (Scotland, UK) - permanent position! Please get in touch with me to find out more.
Light Microscopy Specialist at the University of Dundee
Reference Number: SLSC0470
Job Title: Light Microscopy Specialist
School: Life Sciences
Unit: DIF
Grade: Grade 7 (£31,604 - £38,833)
Closing Date: 07 October 2018
The Dundee Imaging Facility provides a world-leading core microscopy resource to Researchers, Staff and Students in the life, biomedical, and physical sciences, and across the University. . The Facility houses } 20 advanced light microscopy systems, including LSCM, WF-decon, SDCM, TIRF, MPLSM, FLIM and 3DSIM technologies. New acquisitions include a diSPIM and AiryScan systems. The Facility also houses electron microscopy and atomic probe microscopy, which are used for research applications in the life and physical sciences.
We are recruiting for an exceptional individual to join us as a Light Microscopy Specialist within the facility to provide high level technical/specialist expertise and to translate knowledge of advances in the field of advanced imaging/light microscopy into research success.
The role holder will provide user training and support for advanced microscopy systems housed in the facility including FLIM, TIRF, MPLSM, 3DSIM and SR LSCM. They will also assist users to troubleshoot imaging experiments and proactively offer advice and support to solve problems quickly. The role holder will work across both the School of Life Sciences (City Campus) and the School of Medicine (Ninewells Campus).
This is an excellent opportunity to join the Dundee Imaging Facility in a key role supporting the research and training activities of the University. The Facility is a critical resource for staff working in a wide range of disciplines in the Schools of Life Sciences, Medicine and Science & Engineering.
Who were looking for:
Advanced knowledge of all aspects of Light Microscopy Significant experience in advanced imaging Problem solving ability and able to work autonomously and clearly communicate complex, specialist information Independent thinking skills, utilising information obtained from published literature, collaborative discussions, and other sources Strong interpersonal, communication and presentation skills Appreciation and understanding of data protection, confidentiality, equality and diversity legislation Knowledge of all aspects of Health & Safety, Good Laboratory Practice and Research Integrity
We are one of the UKs leading universities internationally recognised for our expertise across a range of disciplines and research breakthroughs in multiple areas, including science, medicine and engineering, amongst many others. Conveniently located on the banks of River Tay, our main city-centre campus is at the heart of Dundee - an up-and-coming, friendly, compact and affordable city with a rich heritage in design and technology. Just a short walk from the V&A Museum of Design Dundee, were close to both the train and bus stations. We also have campuses at Ninewells Hospital and in Kirkcaldy which are easily accessible via local transport links.
For further information about this position please contact Paul Appleton at p.l.appleton-at-dundee.ac.uk. To find out more about the Dundee Imaging Facility please visit www.lifesci.dundee.ac.uk/technologies/dundee-imaging-facility.
We anticipate that interviews for this appointment will take place in late October.
The diversity of our staff and students helps to make the University of Dundee one of the top universities in the UK. Family friendly policies, staff support networks for BME and LGBT staff, membership of Athena SWAN and Stonewall, as well a full range of disability services, create an enjoyable and inclusive place to work.
Take a lesson from NION-The NION microscopes do not have ANY magnification readout-just a scale bar and pixel size embedded in the digital image.
John Mardinly
} On Sep 7, 2018, at 2:45 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=na5y9zfMI5eKLfe8aWTkpgelxX-ulW6qAndG4HuX6Bc&s=U6hideNyDaM_KcdJKh4-LXEJrvm_s6z9syXlwBoN_Rk&e= } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=na5y9zfMI5eKLfe8aWTkpgelxX-ulW6qAndG4HuX6Bc&s=S4vn40yvRVZmzMe8qFzXbS3IQfCvRuCDlA6Yofjlqk4&e= } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } X-from: Stephane Nizet {nizets2-at-yahoo.com} } Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } } } Dear colleagues, } } I am quite sure that I read a discussion about this before but I cannot } find it anymore so I'm asking again, sorry for that. } We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. } The magnification given by the microscope corresponds to the image on } the phosphor screen. } But since the camera is not in the same plane and the beam is not } parallel, the magnification on the camera is different. } } Of course I calibrate the camera so my measurements on pictures taken by } the camera are (more or less) right, but how can I know exactly at which } magnification the picture has been taken? } And, even more importantly, does it matter? } Since the digital pictures can be printed at (almost) any size, this } would mean that I end up with different magnifications, am I right? } } If the microscope tells me that I used a 40 000x magnification, what } should I write in my digital picture if I want to write a magnification } additionally to the scale bar? Or does the question make any sense at all? } } } Stephane } } } } ==============================Original Headers============================== } 14, 53 -- From microscopy.listserver-at-gmail.com Fri Sep 7 16:45:19 2018 } 14, 53 -- Received: from mail-pf1-f178.google.com (mail-pf1-f178.google.com [209.85.210.178]) } 14, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w87LjIPN007303 } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 7 Sep 2018 16:45:19 -0500 } 14, 53 -- Received: by mail-pf1-f178.google.com with SMTP id j8-v6so7604860pff.6 } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 07 Sep 2018 14:44:55 -0700 (PDT) } 14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=gmail.com; s=20161025; } 14, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 14, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 14, 53 -- bh=xGjzHAhwALgAJ3XUpMCCFonBgMliCK41auGM4mTx+5k=; } 14, 53 -- b=DphOLep62bknJg2lWMTvcEVbUvYmKFBcppzs78dz29WW+cI9i6MuuVFiB062nlAbPe } 14, 53 -- SP1l9abN4X6w76cRCbuhBtlyUj3pphd2fo0Y0MRHFaEgfd97QSnp1o59XsQKdQc4oq7k } 14, 53 -- 1+G1gWZz/RCN4CP0/7VFFIYsSvs36x9skRZ90g+hZqo7AJRkHKgv2sKMQyX//FYpeZAH } 14, 53 -- NKiQVVkLbKogxmn4mHBJLBmuBjHBIPCgzXK0p4zm+9Cg/YQCJl1Zdvc+k2+15gxuzW/A } 14, 53 -- T3BQPtqt7iRLqlO5xTfTZwOvheWdDfmNO8P//zY5D7/w0zzQU1LZHg9GUB5M/kBTkpa3 } 14, 53 -- uFNQ== } 14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=1e100.net; s=20161025; } 14, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 14, 53 -- :user-agent:mime-version:in-reply-to:content-language } 14, 53 -- :content-transfer-encoding; } 14, 53 -- bh=xGjzHAhwALgAJ3XUpMCCFonBgMliCK41auGM4mTx+5k=; } 14, 53 -- b=Rg4CVA6vhYXMdic/5/uGKIv3VSwSemGnjiWuA+xaTDTk1b8+cSn6SIiMqoK+/ryCU/ } 14, 53 -- ZQ9XNmJtpWocQQqBTrgn0UN+CJWn7l4bb7L/fnW1zA0NAei8H4QM3KIa0cvdmwPCqUJL } 14, 53 -- hsZHX3L4FM7GgnsZoPa/dG7Y5deHs1vSFrzgBuI8t/H8IbTf4+//WWJSIw0Vv0zqNLqA } 14, 53 -- 0WB7sKJPAtInkLy7Sj3BDl/ps9fIeHoUFmDPFck4Khks4Wrn1v3TVBCAusTZ1xISz6Yc } 14, 53 -- XXW7DIGZBv/uKVd4l4SH26LxfIYMdylu6R0N6vksb6xBr8RCCaX/TzYCnjjoc9aiuG9M } 14, 53 -- xdSA== } 14, 53 -- X-Gm-Message-State: APzg51C3ReVboY6BGnLJUR2kuDV85ClljUSRScpaKearG1bSLxUwmkEg } 14, 53 -- yNNre5xq68A8uYyqLudNEXe2pTZI } 14, 53 -- X-Google-Smtp-Source: ANB0VdbpJ5uFi+3WCT2YmqkVUVckMUIBQd9GdKq1XC+XBH06WEC59TblV4GTnzXtYUDRt2jAUkjyKw== } 14, 53 -- X-Received: by 2002:a63:f44d:: with SMTP id p13-v6mr10634195pgk.257.1536356695157; } 14, 53 -- Fri, 07 Sep 2018 14:44:55 -0700 (PDT) } 14, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([119.17.50.218]) } 14, 53 -- by smtp.googlemail.com with ESMTPSA id d66-v6sm13427339pfd.121.2018.09.07.14.44.53 } 14, 53 -- for {microscopy-at-microscopy.com} } 14, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 14, 53 -- Fri, 07 Sep 2018 14:44:54 -0700 (PDT) } 14, 53 -- Subject: Fwd: magnification on a side-mounted camera } 14, 53 -- References: {790790832.295767.1536213203616-at-mail.yahoo.com} } 14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 14, 53 -- X-Forwarded-Message-Id: {790790832.295767.1536213203616-at-mail.yahoo.com} } 14, 53 -- Message-ID: {6bd084dd-81a0-a388-fd7f-d831656f9e09-at-gmail.com} } 14, 53 -- Date: Sat, 8 Sep 2018 07:44:50 +1000 } 14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:52.0) } 14, 53 -- Gecko/20100101 Thunderbird/52.9.1 } 14, 53 -- MIME-Version: 1.0 } 14, 53 -- In-Reply-To: {790790832.295767.1536213203616-at-mail.yahoo.com} } 14, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 14, 53 -- Content-Language: en-US } 14, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}
The magnification dpends on where you want to know You can easily calculate from pixel size on the ccd sensor Another possibily is where you read the image. Off course it depends of your monitor size. Usually it is better to tell the final magnification and the magnification on the sensor Anyway, the microbar Will be the best for mag calibration
Best regards Michel
Envoy de mon Galaxy A5 2017 Orange
-------- Message d'origine -------- De : microscopy.listserver-at-gmail.com Date : 07/09/2018 23:45 (GMT+01:00) : Ribardire Michel {m.ribardiere-at-jeol.fr} Objet : ***SPAM-PROBABLE*** [Microscopy] Fwd: magnification on a side-mounted camera
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I am quite sure that I read a discussion about this before but I cannot find it anymore so I'm asking again, sorry for that. We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. The magnification given by the microscope corresponds to the image on the phosphor screen. But since the camera is not in the same plane and the beam is not parallel, the magnification on the camera is different.
Of course I calibrate the camera so my measurements on pictures taken by the camera are (more or less) right, but how can I know exactly at which magnification the picture has been taken? And, even more importantly, does it matter? Since the digital pictures can be printed at (almost) any size, this would mean that I end up with different magnifications, am I right?
If the microscope tells me that I used a 40 000x magnification, what should I write in my digital picture if I want to write a magnification additionally to the scale bar? Or does the question make any sense at all?
I would print the picture, measure the scale bar with a ruler, convert to micrometers and divide by the number of micrometers indicated by the scale.
Best,
AMALIA
On Fri, Sep 7, 2018 at 5:50 PM, {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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I am quite sure that I read a discussion about this before but I cannot find it anymore so I'm asking again, sorry for that. We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. The magnification given by the microscope corresponds to the image on the phosphor screen. But since the camera is not in the same plane and the beam is not parallel, the magnification on the camera is different.
Of course I calibrate the camera so my measurements on pictures taken by the camera are (more or less) right, but how can I know exactly at which magnification the picture has been taken? And, even more importantly, does it matter? Since the digital pictures can be printed at (almost) any size, this would mean that I end up with different magnifications, am I right?
If the microscope tells me that I used a 40 000x magnification, what should I write in my digital picture if I want to write a magnification additionally to the scale bar? Or does the question make any sense at all?
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
I am quite sure that I read a discussion about this before but I cannot find it anymore so I'm asking again, sorry for that. We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. The magnification given by the microscope corresponds to the image on the phosphor screen. But since the camera is not in the same plane and the beam is not parallel, the magnification on the camera is different.
Of course I calibrate the camera so my measurements on pictures taken by the camera are (more or less) right, but how can I know exactly at which magnification the picture has been taken? And, even more importantly, does it matter? Since the digital pictures can be printed at (almost) any size, this would mean that I end up with different magnifications, am I right?
If the microscope tells me that I used a 40 000x magnification, what should I write in my digital picture if I want to write a magnification additionally to the scale bar? Or does the question make any sense at all?
X-from: Christopher Winkler {microwink-at-gmail.com}
Hello Stephane,
In Digital Micrograph, you can look at the image tags and it will display the actual magnification of an image captured using a camera located above or below the film chamber. I'm sure your camera uses different software, but the magnification may be embedded in the image tags as well. You can check with the vendor. You can also calculate the difference in magnification by using the markings on the green screen with a calibration sample or diffraction pattern. There are other ways to compute magnification, and I'm sure you will receive replies that are more helpful in that regard.
Ultimately, magnification is somewhat meaningless. The total field of view (FOV) in physical units, the scale bar, and/or the pixel size in physical units are much more important. As you point out, magnification is relative. The physical size of the objects in your images should not be. Generally a scale bar combined with the FOV is enough for most, but it depends on your customer's needs.
I hope this was somewhat helpful. Cheers, Chris
On Fri, Sep 7, 2018 at 5:48 PM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } X-from: Stephane Nizet {nizets2-at-yahoo.com} } Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } } } Dear colleagues, } } I am quite sure that I read a discussion about this before but I cannot } find it anymore so I'm asking again, sorry for that. } We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. } The magnification given by the microscope corresponds to the image on } the phosphor screen. } But since the camera is not in the same plane and the beam is not } parallel, the magnification on the camera is different. } } Of course I calibrate the camera so my measurements on pictures taken by } the camera are (more or less) right, but how can I know exactly at which } magnification the picture has been taken? } And, even more importantly, does it matter? } Since the digital pictures can be printed at (almost) any size, this } would mean that I end up with different magnifications, am I right? } } If the microscope tells me that I used a 40 000x magnification, what } should I write in my digital picture if I want to write a magnification } additionally to the scale bar? Or does the question make any sense at all? } } } Stephane } } } } ==============================Original Headers============================== } 14, 53 -- From microscopy.listserver-at-gmail.com Fri Sep 7 16:45:19 2018 } 14, 53 -- Received: from mail-pf1-f178.google.com (mail-pf1-f178.google.com [209.85.210.178]) } 14, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w87LjIPN007303 } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 7 Sep 2018 16:45:19 -0500 } 14, 53 -- Received: by mail-pf1-f178.google.com with SMTP id j8-v6so7604860pff.6 } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 07 Sep 2018 14:44:55 -0700 (PDT) } 14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=gmail.com; s=20161025; } 14, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 14, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 14, 53 -- bh=xGjzHAhwALgAJ3XUpMCCFonBgMliCK41auGM4mTx+5k=; } 14, 53 -- b=DphOLep62bknJg2lWMTvcEVbUvYmKFBcppzs78dz29WW+cI9i6MuuVFiB062nlAbPe } 14, 53 -- SP1l9abN4X6w76cRCbuhBtlyUj3pphd2fo0Y0MRHFaEgfd97QSnp1o59XsQKdQc4oq7k } 14, 53 -- 1+G1gWZz/RCN4CP0/7VFFIYsSvs36x9skRZ90g+hZqo7AJRkHKgv2sKMQyX//FYpeZAH } 14, 53 -- NKiQVVkLbKogxmn4mHBJLBmuBjHBIPCgzXK0p4zm+9Cg/YQCJl1Zdvc+k2+15gxuzW/A } 14, 53 -- T3BQPtqt7iRLqlO5xTfTZwOvheWdDfmNO8P//zY5D7/w0zzQU1LZHg9GUB5M/kBTkpa3 } 14, 53 -- uFNQ== } 14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=1e100.net; s=20161025; } 14, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 14, 53 -- :user-agent:mime-version:in-reply-to:content-language } 14, 53 -- :content-transfer-encoding; } 14, 53 -- bh=xGjzHAhwALgAJ3XUpMCCFonBgMliCK41auGM4mTx+5k=; } 14, 53 -- b=Rg4CVA6vhYXMdic/5/uGKIv3VSwSemGnjiWuA+xaTDTk1b8+cSn6SIiMqoK+/ryCU/ } 14, 53 -- ZQ9XNmJtpWocQQqBTrgn0UN+CJWn7l4bb7L/fnW1zA0NAei8H4QM3KIa0cvdmwPCqUJL } 14, 53 -- hsZHX3L4FM7GgnsZoPa/dG7Y5deHs1vSFrzgBuI8t/H8IbTf4+//WWJSIw0Vv0zqNLqA } 14, 53 -- 0WB7sKJPAtInkLy7Sj3BDl/ps9fIeHoUFmDPFck4Khks4Wrn1v3TVBCAusTZ1xISz6Yc } 14, 53 -- XXW7DIGZBv/uKVd4l4SH26LxfIYMdylu6R0N6vksb6xBr8RCCaX/TzYCnjjoc9aiuG9M } 14, 53 -- xdSA== } 14, 53 -- X-Gm-Message-State: APzg51C3ReVboY6BGnLJUR2kuDV85ClljUSRScpaKearG1bSLxUwmkEg } 14, 53 -- yNNre5xq68A8uYyqLudNEXe2pTZI } 14, 53 -- X-Google-Smtp-Source: ANB0VdbpJ5uFi+3WCT2YmqkVUVckMUIBQd9GdKq1XC+XBH06WEC59TblV4GTnzXtYUDRt2jAUkjyKw== } 14, 53 -- X-Received: by 2002:a63:f44d:: with SMTP id p13-v6mr10634195pgk.257.1536356695157; } 14, 53 -- Fri, 07 Sep 2018 14:44:55 -0700 (PDT) } 14, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([119.17.50.218]) } 14, 53 -- by smtp.googlemail.com with ESMTPSA id d66-v6sm13427339pfd.121.2018.09.07.14.44.53 } 14, 53 -- for {microscopy-at-microscopy.com} } 14, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 14, 53 -- Fri, 07 Sep 2018 14:44:54 -0700 (PDT) } 14, 53 -- Subject: Fwd: magnification on a side-mounted camera } 14, 53 -- References: {790790832.295767.1536213203616-at-mail.yahoo.com} } 14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 14, 53 -- X-Forwarded-Message-Id: {790790832.295767.1536213203616-at-mail.yahoo.com} } 14, 53 -- Message-ID: {6bd084dd-81a0-a388-fd7f-d831656f9e09-at-gmail.com} } 14, 53 -- Date: Sat, 8 Sep 2018 07:44:50 +1000 } 14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:52.0) } 14, 53 -- Gecko/20100101 Thunderbird/52.9.1 } 14, 53 -- MIME-Version: 1.0 } 14, 53 -- In-Reply-To: {790790832.295767.1536213203616-at-mail.yahoo.com} } 14, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 14, 53 -- Content-Language: en-US } 14, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- Senior Research Associate Nanoscale Characterization and Fabrication Laboratory Institute for Critical Technology and Applied Science Virginia Tech (267) 496-0587
I agree with John Mardinly that a scale bar (preferably as an overlay in the image) and pixel size embedded (I'm assuming in the metadata) in the digital image are a minimum requirement for image recording.
I do like the idea of including a "nominal magnification" in the metadata as well. That is really only the instrument setting. I always found it useful to be able to record additional images under the same conditions. I always liked the way Gatan recorded all of the settings the microscope could measure (and a few input by the user at acquisition time) in the metadata in images from DigitalMicrographs. As many in the #rstats community are fond of noting, "your closest collaborator is you six months from now and you don't respond to email." Metadata saved my bacon many times...
Best regards, John Minter
On Sat, Sep 8, 2018 at 12:26 PM {John.Mardinly-at-asu.edu} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Take a lesson from NION-The NION microscopes do not have ANY magnification readout-just a scale bar and pixel size embedded in the digital image. } } John Mardinly } } } On Sep 7, 2018, at 2:45 PM, microscopy.listserver-at-gmail.com wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=na5y9zfMI5eKLfe8aWTkpgelxX-ulW6qAndG4HuX6Bc&s=U6hideNyDaM_KcdJKh4-LXEJrvm_s6z9syXlwBoN_Rk&e= } } On-Line Help https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.com_MicroscopyListserver_FAQ.html&d=DwIBAg&c=l45AxH-kUV29SRQusp9vYR0n1GycN4_2jInuKy6zbqQ&r=VLPJ8OE-c_C6joGeE1ftlvxMmQPq9N6mpKZONBRt90E&m=na5y9zfMI5eKLfe8aWTkpgelxX-ulW6qAndG4HuX6Bc&s=S4vn40yvRVZmzMe8qFzXbS3IQfCvRuCDlA6Yofjlqk4&e= } } ---------------------------------------------------------------------------- } } } } } } } } } } -------- Forwarded Message -------- } } } } X-from: Stephane Nizet {nizets2-at-yahoo.com} } } Reply-To: Stephane Nizet {nizets2-at-yahoo.com} } } } } } } Dear colleagues, } } } } I am quite sure that I read a discussion about this before but I cannot } } find it anymore so I'm asking again, sorry for that. } } We have a side-mounted camera (Megaview III) on a tecnai G20 microscope. } } The magnification given by the microscope corresponds to the image on } } the phosphor screen. } } But since the camera is not in the same plane and the beam is not } } parallel, the magnification on the camera is different. } } } } Of course I calibrate the camera so my measurements on pictures taken by } } the camera are (more or less) right, but how can I know exactly at which } } magnification the picture has been taken? } } And, even more importantly, does it matter? } } Since the digital pictures can be printed at (almost) any size, this } } would mean that I end up with different magnifications, am I right? } } } } If the microscope tells me that I used a 40 000x magnification, what } } should I write in my digital picture if I want to write a magnification } } additionally to the scale bar? Or does the question make any sense at all? } } } } } } Stephane } } } } } } } } ==============================Original Headers============================== } } 14, 53 -- From microscopy.listserver-at-gmail.com Fri Sep 7 16:45:19 2018 } } 14, 53 -- Received: from mail-pf1-f178.google.com (mail-pf1-f178.google.com [209.85.210.178]) } } 14, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w87LjIPN007303 } } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 7 Sep 2018 16:45:19 -0500 } } 14, 53 -- Received: by mail-pf1-f178.google.com with SMTP id j8-v6so7604860pff.6 } } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 07 Sep 2018 14:44:55 -0700 (PDT) } } 14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } } 14, 53 -- d=gmail.com; s=20161025; } } 14, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } } 14, 53 -- :in-reply-to:content-language:content-transfer-encoding; } } 14, 53 -- bh=xGjzHAhwALgAJ3XUpMCCFonBgMliCK41auGM4mTx+5k=; 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2. The invited speakers include: (plenary) John Randall, Zyvex (plenary) Toma Susi, U. Vienna David Forrest, DOE EERE Khershed Cooper, /NSF / Ondrej Krivanek, Nion Rick Silver, NIST Stephen Jesse, ORNL Adam Schwartzberg, Molecular Foundry Dirk Englund, MIT Marija Drndic, UPenn Ezra Bussman, Sandia Prineha Narang, Harvard Joe Lyding, U. Illinois Q. Ramasse, SuperSTEM Travis Humble, ORNL Aaron Stein, CFN BNL R. Wolkow, U. Alberta
3. There are slots available for several invited, contributed, and poster presentations!
Looking forward to hearing from you!
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 12, 37 -- From sergei2-at-ornl.gov Wed Sep 12 09:53:33 2018 12, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 12, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w8CErWme002760 12, 37 -- for {microscopy-at-microscopy.com} ; Wed, 12 Sep 2018 09:53:32 -0500 12, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 12, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 12, 37 -- t=1536764005; x=1568300005; 12, 37 -- h=to:from:subject:message-id:date:mime-version: 12, 37 -- content-transfer-encoding; 12, 37 -- bh=AWoCGpVuOXAfV/YseZHBgkkRf37m8PElVVcCghOT5O0=; 12, 37 -- b=KDIy3cY6b/lvEHvW3qLqMlUeWlYnlWemom3d+kpRQIFT6UU3KlV1RH9k 12, 37 -- LCrB0fGuqKEUe7vTvK7/ObJPY41nwwztFPJNSRZRvCmxR+UT2ck+J/z7C 12, 37 -- X7RAYF7HwocEcYWvvG5XQVdwct9wnZVj78H96YJQOTXmUMq6pjTof50MO 12, 37 -- 4/wMCdz/EteBYnV5Gv2QVxqqEZN118KOGcJ/beFGNhN5MX+OjGLAcJFP3 12, 37 -- iQv6TXVHQHbLQSi2mG7/RV60LTet2Ukyy+ZI6YYng2oRB83JZVzLL/1DT 12, 37 -- 90a7v3HxrKRzZxzd5JU5Oy6tkDI5HtHyKMxNnREFHoDUKDlxEUaBL/vKZ 12, 37 -- w==; 12, 37 -- X-SG: RELAYLIST 12, 37 -- X-IronPort-AV: E=Sophos;i="5.53,365,1531800000"; 12, 37 -- d="scan'208";a="46621491" 12, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 12, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 12 Sep 2018 10:53:24 -0400 12, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 12, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 12, 37 -- (No client certificate requested) 12, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 429Psw5HZNz2Sr9G 12, 37 -- for {microscopy-at-microscopy.com} ; Wed, 12 Sep 2018 10:53:24 -0400 (EDT) 12, 37 -- To: microscopy-at-microscopy.com 12, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 12, 37 -- Subject: November 1,2: Atom by atom fabrication via STEM electron beams 12, 37 -- Message-ID: {5B992864.3040406-at-ornl.gov} 12, 37 -- Date: Wed, 12 Sep 2018 10:53:24 -0400 12, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 12, 37 -- Thunderbird/38.0.1 12, 37 -- MIME-Version: 1.0 12, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 12, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Email: glaevsky.lists-at-gmail.com Name: Gary Laevsky
Organization: North Atlantic Microscopy Society (NAMS)
Title-Subject: [Filtered] North Atlantic Microscopy Society (NAMS); Inaugural Symposium November 1, 2018 at Princeton University
Message: Hello!
For registration, https://namsmicroscopy.com/meeting-registration
We are very proud and excited to announce the formation of a new society, The North Atlantic Microscopy Society (NAMS). Geographically centered at Princeton, NJ, we envision our coverage to span southern New York, New Jersey and into Pennsylvania. NAMS was born not simply because we noticed a distinct gap in regional cohesion. But because we are passionate about microscopy in all its forms and believe that we are not alone. Edwin Hubble famously said, Equipped with his five senses, man explores the universe around him and calls the adventure Science. We believe that some of this exploratory instinct has been muted lately by our disciplinary silos. Individually, we have become exceptional in our specialties and do not take moments to appreciate the many discoveries happening across the entire spectrum of science. Our mission is to bridge these silos through the lens of microscopy. We seek to achieve this mission by promoting microscopy education, stimulating networking among microscopists, and disseminating microscopy knowledge and skills to the public in the region. Our first Symposium will be held at Princeton University on November 1, 2018. We are planning bi-annual symposia, with the fall event always held at Princeton, and the spring event held at rotating locations throughout the region (April '19 tentatively being held at Rockefeller University). We are also planning smaller satellite meetings throughout the academic year. Registration payment covers both symposium and satellite meetings.
Please see below for schedule. We have a full day planned!
And, if you present a poster or a 5 minute lightning talk, the event is FREE!!!
Abstracts accepted on registration page of website namsmicroscopy.com
8:15a Registration/Breakfast 9a-9:30a Opening Remarks 9:30a-10:30a EM talk Esther Bullitt Boston University 10:30a-10:45a Break 10:45a-12p Break out into two rooms 6-5 min Light talks 6-5 min EM talks 12p-2:30p Lunch/vendor session/posters/PRISM tour 2:30p-3:30p Keynote/Light talk Hari Shroff - NIH 3:30p-3:45p Break 3:45p-5p Panel discussions Cryo-EM sample prep Mass Spec imaging Lightsheet imaging Big Data 5p- Organizational/Administrative (pizza/beverages) (WE need help!!!) Treasurer Web Design Secretary
NAMS Co-Founders Gary Laevsky Paul Shao Tharan Srikumar
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop again!
Message: Our Biological TEM workshop is filling up but we have a few seats left! This hands-on, three day workshop is W-F, Oct 24-26, 2018 covers conventional TEM chemical prep, briefly discusses older Cryo techniques, negative staining, sectioning and other topics suggested by you to help you get your work done, and/or learn a new skill.
We have a ton of fun while working hard for three days. For more information, contact John at jphield-at-uga.edu or go to our website for more information and registration (https://gem.uga.edu/biological-tem-workshop/)
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Email: whiteto-at-missouri.edu Name: Tommi A. White
Organization: University of Missouri Electron Microscopy Core
Title-Subject: [Filtered] Workshop Announcement: Machine Learning approaches in High Resolution Microscopy Imaging at BIBM 2018
Message:
We will be hosting a day-long workshop on Machine Learning approaches in High Resolution Microscopy Imaging (MLHRM 2018 - http://www.mlhrm.net/) at the 2018 IEEE International Conference on Bioinformatics and Biomedicine December 3-6, 2018 in Madrid, Spain (http://orienta.ugr.es/bibm2018/).
This workshop aims to bring the researchers from computational and imaging fields together to focus on the computational approaches for construction and analysis in high resolution imaging modalities. Special emphasis will be on the applications of deep learning and other machine learning methods in imaging modalities such as cryo-electron microscopy, FIB-SEM tomography, and fluorescence super-resolution microscopy.
Topics include: * Noise reduction in electron microscopy (EM) & super-resolution microscopy (SRM) modalities * Signal detection and image reconstruction in SRM * Image segmentation in EM modalities * Image classification in EM modalities and SRM * 3D reconstruction methods and pipelines in cryo-EM * Characterization of single molecule structure using machine learning approaches * Applications of high resolution microscopy in precision medicine * Applications in single molecule tracking, multiphoton imaging
Original contributions in applications of deep learning and other machine learning methods in high resolution microscopy including but not limited to noise reduction, detection, segmentation, classification, and reconstruction of 2D and 3D models, as well as new approaches in 3D reconstruction of single molecules are welcome. Contributions regarding other related modalities and automated analysis methods such as single molecule tracking, multiphoton imaging, and combining electron microscopy (EM) with super-resolution microscopy (STORM/PALM) will also be considered for inclusion in the workshop program. Please submit a full-length original and unpublished research contribution (up to 6 pages in IEEE 2-column format) through the online submission system. Formatting instructions for paper submission are available here: http://www.ieee.org/conferences_events/conferences/publishing/templates.html Paper submission deadline has been extended to October 5th, 2018 and can be made here: https://wi-lab.com/cyberchair/2018/bibm18/index.php
We look forward to seeing you in Madrid! Please contact us with any questions (whiteto-at-missouri.edu).
Dr. Tommi White, Electron Microscopy Core & Department of Biochemistry, University of Missouri, USA. Dr. Filiz Bunyak, Department of Electrical Engineering and Computer Science, University of Missouri, USA. Dr. Ilker Ersoy, Informatics Institute, Department of Pathology and Anatomical Sciences, University of Missouri, USA.
Tommi A. White, Ph.D. Assistant Professor of Biochemistry Director, Electron Microscopy Core Facility University of Missouri W117 Veterinary Medicine Building 1600 East Rollins Street Columbia, MO 65211 573-882-8304 WhiteTo-at-missouri.edu http://emc.missouri.edu on here
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Organization: Max Planck Florida Institute for Neuroscience
Title-Subject: [Filtered] CLEM Seminar at Max Planck Florida Institute
Message: The Electron Microscopy Facility of the Max Planck Florida Institute for Neuroscience (Jupiter, FL) will host a new labs-at-location partnership launch event on October 11th, 2018. The event includes a seminar focusing on the latest topics of Correlative Light-Electron Microscopy (CLEM), a demo for the new ZEISS Focal Charge Compensation module on the 3View-Gemini SEM300 system in the EM facility, and a partnership signing ceremony followed by a reception.
The event is open to all levels of researchers who are interested in utilizing the latest advances in electron microscopy. For more details and registration, please check our website; http://mpfi.org/mpfi-zeiss-partnership
Registration is open until October 4th.
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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD
Organization: Kimberly-Clark
Title-Subject: [Filtered] For Donation: TMC Vibration Cancellation Table
Message: We have for donation a Vibration Cancellation Table manufactured by TMC with a 2" steel laminate tabletop. Dimensions: 60" W x 36" D x 30.5" H
Located in Wisconsin Taker must present tax exemption number for their organization and arrange pickup or shipping
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Dear All, I am looking for an automated solution to scan large image arrays with a Quanta 200 FEG-SEM. Any ideas? It should be not very costly since it is for student work...
Thanks, Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
If your Quanta already has SW option for being controlled remotely, then then MicroManager plugin for ImageJ could be something to look at:
https://micro-manager.org/
Another option would be trying to use either AutoIt, or WinAutomation, or similar windows-scripting tool to simulate operator clicking on buttons of Xp GUI for doing repetitive move-image-save operations.
All other solutions I can think of wouldn't fall into "not very costly" category, as they would require either purchasing software from the OEM (if available) or installing third-party scanning stage, etc...
Happy Scanning! Valery
Valery Ray www.linkedin.com/in/valeryray/ Also with REFINE Center, UCONN ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 (leave a message) E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 9/21/2018 8:55 AM, stefan.diller-at-t-online.de wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } I am looking for an automated solution to scan large image arrays with a Quanta 200 FEG-SEM. } Any ideas? } It should be not very costly since it is for student work... } } Thanks, } Stefan } } } } ----------------------------------------------------- } Stefan Diller - Scientific Photography } Arndtstrasse 22 } D - 97072 Wuerzburg Germany } ++49-931-7848700 Phone } ++49-931-7848701 Fax } ++49-175-7177051 Mobile } } Websites: } www.nanoflight.info. NEW! } www.stefan-diller.com } www.electronmicroscopy.info } www.elektronenmikroskopie.info } www.assisi.de } www.zwillingsprojekt.de } ----------------------------------------------------- } } } ==============================Original Headers============================== } 7, 23 -- From stefan.diller-at-t-online.de Fri Sep 21 07:55:41 2018 } 7, 23 -- Received: from mailout04.t-online.de (mailout04.t-online.de [194.25.134.18]) } 7, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w8LCte9a031825 } 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Sep 2018 07:55:41 -0500 } 7, 23 -- Received: from fwd00.aul.t-online.de (fwd00.aul.t-online.de [172.20.26.147]) } 7, 23 -- by mailout04.t-online.de (Postfix) with SMTP id 54D1C419645E } 7, 23 -- for {microscopy-at-microscopy.com} ; Fri, 21 Sep 2018 14:56:02 +0200 (CEST) } 7, 23 -- Received: from [10.35.96.10] (GiOOj+ZpQhZx2bstl93Ha41M2WJUJnmm5BtAs6JyYsvYIfevHyB6S2IaaVHccsUgeb-at-[82.113.106.10]) by fwd00.t-online.de } 7, 23 -- with (TLSv1:ECDHE-RSA-AES256-SHA encrypted) } 7, 23 -- esmtp id 1g3KyW-4Az2qe0; Fri, 21 Sep 2018 14:56:00 +0200 } 7, 23 -- Subject: Automated scanning and stitching with Quanta 200 FEG-SEM } 7, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de} } 7, 23 -- Content-Type: text/plain; } 7, 23 -- charset=us-ascii } 7, 23 -- X-Mailer: iPad Mail (13G36) } 7, 23 -- Message-Id: {CE6E5447-4C6E-4119-9D4B-BFA5E7CAED74-at-t-online.de} } 7, 23 -- Date: Fri, 21 Sep 2018 14:55:55 +0200 } 7, 23 -- To: microscopy-at-microscopy.com } 7, 23 -- Mime-Version: 1.0 (1.0) } 7, 23 -- X-ID: GiOOj+ZpQhZx2bstl93Ha41M2WJUJnmm5BtAs6JyYsvYIfevHyB6S2IaaVHccsUgeb } 7, 23 -- X-TOI-MSGID: 752662fd-6331-4629-930e-3835406d3032 } 7, 23 -- Content-Transfer-Encoding: 8bit } 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w8LCte9a031825 } ==============================End of - Headers============================== }
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Message: The Mountain States Society of Electron Microscopists is hosting a dinner meeting Oct 3rd from 5p-9p in Westminster Colorado.
PLEASE REGISTER BY SEPTEMBER 25! https://www.eventbrite.com/e/mssemcomas-fall-2018-dinner-meeting-tickets-49235800605
Join us for dinner and stimulating conversation with presentations by distinguished scientists!
5:00 - 6:00 Check in and mingle
6:00 - 6:45 Dinner with short announcements from vendors
6:45 - 7:30 Dr. Dale Newbury (NIST), Electron-excited Energy Dispersive X-ray Spectrometry: 50 years of Progress in 30 minutes! (Heather Lowers presenting)
7:30 - 7:45 Local Society updates, break and dessert served
7:45 - 8:30 Dr. Masashi Watanabe (Lehigh University) Challenges of Atomic Resolution X-ray Analysis in (Scanning) Transmission Electron Microscopes
8:30 - 9:00 Wrap up and adjourn
Cash bar will be available.
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Email: daniel.vanhart-at-uic.com Name: Daniel VanHart
Message: We are having difficulty reaching the "vac OK" state on the SEM. Typically, we would reach it in a few minutes but something changed dramatically and now it takes more than 15 minutes and sometimes it never gets there. Can anyone suggest what to check first?
Any suggestions for troubleshooting would be appreciated.
Thank you,
Daniel VanHart
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My lab (I'm now retired) had an FEI Sirion built on a similar column. I saw this many times. The usual culprit was degradation of the vacuum hoses around the connectors. They would develop cracks and need to be replaced. I would check there first. You don't say whether your pumps are mechanical or (dry) scroll pumps. Each of these need periodic maintenance. Follow the manufacturer's instructions.
Hope this helps.
Best regards, John Minter
} Email: daniel.vanhart-at-uic.com Name: Daniel VanHart } Title-Subject: [Filtered] FEI XL-30 ESEM Poor vacuum } } Message: We are having difficulty reaching the "vac OK" state on the SEM. Typically, we would reach } it in a few minutes but something changed dramatically and now it takes more than 15 minutes and } sometimes it never gets there. } Can anyone suggest what to check first? } } Any suggestions for troubleshooting would be appreciated. } } Thank you, } } Daniel VanHart
Forwarding for a colleague. Do NOT reply to me! Phil
Vinayak P. Dravid Abraham Harris Chaired Professor Department of Materials Science & Engineering Founding Director, NUANCE Center Founding Director; SHyNE Resource (NNCI) Co-Director, Global McCormick Fellow; APS, AAAS, ACerS, MSA & MRS Email:v-dravid-at-northwestern.edu Website: www.northwestern.edu/vpdgroup Phone 847-491-3537 -at-profdravid www.facebook.com/NUANCE.Center/ IMMEDIATE OPEN POSITION - Postdoctoral Research Associate Cryo-Electron Microscopy of Biomolecular Structures and Dynamics NORTHWESTERN UNIVERSITY A postdoctoral research associate position is immediately available at Northwestern University in the area of “Cryo Electron Microscopy” of proteins, related biomolecular structures, complexes and their conformational dynamics under external stimuli. The candidate will work in the group of Professor Vinayak Dravid in the department of materials science & engineering, towards the 3D and 4D characterization of structures and dynamics of fusion proteins, megamolecules and related complexes, primarily by cryo and conventional scanning transmission and transmission electron microscopy (S/TEM). Select experiments will be performed with fluidic-cell scanned probe microscopy (FC-SPM) to probe dynamic conformation changes and local nanomechanical perturbations. The candidate will work closely with the NUANCE Center (NSF Center for Facility Excellence in the greater Midwest), which houses a comprehensive sample preparation and atomic-nanoscale characterization capabilities for soft, hard and hybrid structures. Dravid’s research is part of an extensive cross-disciplinary and collaborative project across Chemistry, Biomedical Engineering, and Cell & Molecular Biology experts at Northwestern and University of Chicago. As a result, the candidate will have ample opportunity to learn diverse aspects of synthetic biology, simulation and imaging of unconventional molecules. The preferred candidate will have a doctoral degree in science or engineering fields. The preferred candidate will also have a proven track-record and established hands-on experience in the areas of advanced electron microscopy of biomolecules and soft matter. Experience should include extensive expertise and hands-on experience in cryo-EM of biomolecules, in image processing, and in programming/software for the reconstruction of protein based structures. The position is available immediately for two years with the possibility for extension. Compensation will be commensurate with experience and expertise, with opportunities for research faculty appointment towards training and preparation for tenure-track faculty position. Complete applications will include: Introduction letter, Curriculum Vitae, a brief Research Statement, Contact information for 3 references (name, postal & email address, phone number). Materials should be submitted as a single PDF to amy.morgan-at-northwestern.edu and vdravid-at-northwestern.edu
------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office 989 774-7567 lab
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Email: sorin.lazar-at-gmail.com Name: Sorin Lazar
Organization: Thermo Fisher Scientific
Title-Subject: [Filtered] Applications Scientist on TEM Materials Science
Message: Dear Microscopists,
I would like to draw your attention to an opening in the Eindhoven Nanoport at Thermo Fisher Scientific. If you know someone interested it will be very much appreciated if you could please forward the link below.
X-from: Ravi Thakkar {ravi.thakkar369-at-gmail.com}
I had a same problem in my Hitachi SEM, the airline connected to one of the pressure valve was leaking. Just check the all tubing connected to vacuum pump and pressure gauge. There must be some leak somewhere.
With Thanks and Regards. ------------------------------------------------------------------------- Ravindra Thakkar Associate Scientist, Kansas State University, Manhattan, Kansas (USA) -------------------------------------------------------------------------
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Email: daniel.vanhart-at-uic.com {mailto:daniel.vanhart-at-uic.com} Name: Daniel VanHart
Message: We are having difficulty reaching the "vac OK" state on the SEM. Typically, we would reach it in a few minutes but something changed dramatically and now it takes more than 15 minutes and sometimes it never gets there. Can anyone suggest what to check first?
Any suggestions for troubleshooting would be appreciated.
Thank you,
Daniel VanHart
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My colleagues and I would like to bring to your attention the following development aimed at faster adoption of machine learning methods across electron microscopy community, and enable ML/AI application in atomically resolved imaging. Modern machine learning is impossible without large volumes of labeled data. To enable faster adoption of machine learning methods in STEM, ORNL is working with Citrine Informatics to share an open library of images for the specific case of Si - vacancy complexes in graphene monolayers with plans to increase the amount of data in the library over time. The initial library is available at (https://doi.org/10.25920/0xv3-8459)
A paper that discusses the collection, analysis, and dissemination of this data is available at https://arxiv.org/abs/1809.04256 The notebooks for the analysis workflow will be available at PyCroscopy (on GitHub) shortly, and can be requested directly from Maxim Ziatdinov (ziatdinovma-at-ornl.gov {mailto:ziatdinovmax-at-gmail.com} )
We hope that this initiative becomes adopted by the community. Please contact Malcolm Davidson (mdavidson-at-citrine.io {mailto:mdavidson-at-citrine.io} ), the leader of Citrine's Open Data Initiative, for any questions about Citrine's open data repository or ML platform. We're putting the finishing touches on some tooling and will share a step-by-step guide to our workflow in the coming months if you're interested in sharing your STEM data openly on Citrine's platform.
Looking forward to the new opportunities and collaborations
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, Foresight Institute, APS, IoP, AVS
The Hydro-Quebec Center of Excellence, a leader in battery material research, is looking for a microscopist SEM and/or TEM at the technician expert level to work in the characterization group which includes 5 state-of-the-art SEM and TEM and many more instruments. The job is well paid with very good social advantage and work conditions. See the job posting at :
The Hydro-Quebec work environment is mostly in French, but the Center has a lot of international employees and the work environment is in French and English.
Hi, I heard that there has been some effort to archive AMRAY documentation and schematics. I'm curious how that's going as I've got an AMRAY 1845 FE that's new to me and any information I can find would be helpful for understanding their way of doing things.
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Email: mike.toalson-at-elementpi.com Name: Mike Toalson
Organization: Element Pi, LLC
Title-Subject: [Filtered] Looking for EM Service help
Message: Due to our fast growth this year, we are in need of a SEM service engineer in the Northeast US or Great Lakes area.
Can anyone recommend a Third Party EM Service company in these areas?
We would also entertain being contacted by anyone looking for work as a service engineer, installer and trainer for both floor model and tabletop SEM.
Please contact Mike Toalson Element Pi, LLC 833-314-1593 (833-Pi)
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Email: mike.toalson-at-elementpi.com Name: Mike Toalson
Organization: Element Pi, LLC
Title-Subject: [Filtered] SEM Independent Sale Agent opportunity
Message: We are searching for an Independent Sales Agent interested in working with us for the 8 state region surrounding the Great Lakes area ideally based in the Chicago area but other locations within the region are possible. We have both entry level floor model and compact SEM systems as well as sample preparation tools. You will start with an active pipeline and established customers already using our systems in the region.
Background in EM preferred but Optical Microscopy, AFM, SEM accessories and such is perfectly adequate.
Please contact: Mike Toalson Element Pi, LLC 833-314-1593 (833-Pi)
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Email: glaevsky-at-princeton.edu Name: Gary Laevsky
Organization: Princeton University
Title-Subject: [Filtered] Deadline 10/20/2018: North Atlantic Microscopy Society Inaugural Symposium at Princeton University, November 1, 2018
Message: Hi All,
For registration, namsmicroscopy.com/meeting-registration
We are very proud and excited to announce the formation of a new society, The North Atlantic Microscopy Society (NAMS). Geographically centered at Princeton, NJ, we envision our coverage to span southern New York, New Jersey and into Pennsylvania. NAMS was born not simply because we noticed a distinct gap in regional cohesion. But because we are passionate about microscopy in all its forms and believe that we are not alone.
Edwin Hubble famously said, Equipped with his five senses, man explores the universe around him and calls the adventure Science. We believe that some of this exploratory instinct has been muted lately by our disciplinary silos. Individually, we have become exceptional in our specialties and do not take moments to appreciate the many discoveries happening across the entire spectrum of science.
Our mission is to bridge these silos through the lens of microscopy. We seek to achieve this mission by promoting microscopy education, stimulating networking among microscopists, and disseminating microscopy knowledge and skills to the public in the region.
Our first Symposium will be held at Princeton University on November 1, 2018. We are planning bi-annual symposia, with the fall event always held at Princeton, and the spring event held at rotating locations throughout the region (April '19 tentatively being held at Rockefeller University). We are also planning smaller satellite meetings throughout the academic year. Registration payment covers both symposium and satellite meetings.
Please see below for schedule. We have a full day planned!
And, if you present a poster or a 5 minute lightning talk, the event is FREE!!!
Abstracts accepted on registration page of website namsmicroscopy.com
8:15a Registration/Breakfast
9a-9:30a Opening Remarks
9:30a-10:30a EM talk
Esther Bullitt =E2=80=93= Boston University
10:30a-10:45a Break
10:45a-12p Break out into two rooms
6-5 min Light talks
6-5 min EM talk= s
12p-2:30p Lunch/vendor session/posters/PRISM tour
2:30p-3:30p Keynote/Light talk
Hari Shroff - NIH
3:30p-3:45p Break
3:45p-5p Panel discussions
Cryo-EM sample prep
Mass Spec imaging
Lightsheet imaging
Big Data
5p- Organizational/Administrative (pizza/beverages) (WE need help!!!)
Treasurer
Web Design
Secretary
NAMS souvenir tote bags to the first 50 registered attendees!
Speakers (lightning talks) and poster presenters attend free! Looks great on the CV! Sign up now and share your Science!
Spring symposium confirmed at Rockefeller April 5, 2019! Fall 2019 at Princeton. Spring 2010 tentatively held at UPENN!
Almost 20 vendors! 10 vendor talks!
https://namsmicroscopy.com/meeting-registration
NAMS Co-Founders
Gary Laevsky
Paul Shao
Tharan Srikumar
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Email: mdelann1-at-jhmi.edu Name: michael delannoy
Organization: Hopkins SOM Microscope Facility
Title-Subject: [Filtered] Cryo vs std TEM use
Message: Hello, I would like to know the feasibility of having a TEM operate on a weekly basis both as a standard electron microscope and a cryo-electron microscope. Is this practical?
thanks, M Delannoy
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In general it is very possible to use a TEM in cryo or std mode intermixed, if the "cryo" use involves a "cryo holder" and not a fully chilled cryo-stage. A JEOL 3200 cryo, would be a full cryo stage and not switch easily between projects. Using a cryo-holder such as the one Gatan sells, with a LN2 reservoir and cryo shields so that the sample is protected during transfer to the column, it is quite fine. UC Berkeley has been doing cryo-TEM and non-cryo in the same CM series TEM for decades.
Most materials people do not fully understand polymer of biological "cryo" work, so there will be some learning involved. I've been lucky to have participated on the both sides of the TEM world.
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Email: mdelann1-at-jhmi.edu Name: michael delannoy
Organization: Hopkins SOM Microscope Facility
Title-Subject: [Filtered] Cryo vs std TEM use
Message: Hello, I would like to know the feasibility of having a TEM operate on a weekly basis both as a standard electron microscope and a cryo-electron microscope. Is this practical?
thanks, M Delannoy
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} } } 03.10.2018 at 10:58: } I would like to know the feasibility of having a TEM operate on a weekly } basis both as a standard electron microscope and a cryo-electron } microscope. Is this practical?
Dear Michael, There is no general answer, honestly. It depends on the facility, it very much depends on the training of the various users you have / you have to get trained and to supervise, on the equipment, and it depends on the TEM that you have. Yes, we do it, not on weekly basis, but with daily varying schedules, and this works. Fine. Little problems, only, which are due to the limited training of the users, rather than the TEM. If you know exactly what your TEM is able to do and to tolerate, and what your users know, then YES, it is possible. Then, I do not see any reason why a weekly schedule would not work. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 Elected member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz
==============================Original Headers============================== 9, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Wed Oct 3 11:33:46 2018 9, 23 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 9, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w93GXkrb014540 9, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2018 11:33:46 -0500 9, 23 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 9, 23 -- by localhost (Postfix) with SMTP id 3DBB567263 9, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2018 18:34:48 +0200 (CEST) 9, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 9, 23 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id 282B7671DE 9, 23 -- for {Microscopy-at-microscopy.com} ; Wed, 3 Oct 2018 18:34:48 +0200 (CEST) 9, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 9, 23 -- with Novell_GroupWise; Wed, 03 Oct 2018 18:34:48 +0200 9, 23 -- Message-Id: {5BB4EFA6020000540007A45B-at-gwsmtp1.uni-regensburg.de} 9, 23 -- X-Mailer: Novell GroupWise Internet Agent 18.0.2 9, 23 -- Date: Wed, 03 Oct 2018 18:34:46 +0200 9, 23 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 9, 23 -- To: {mdelann1-at-jhmi.edu} , "microscopy server" {Microscopy-at-microscopy.com} 9, 23 -- Subject: Cryo vs std TEM use 9, 23 -- Mime-Version: 1.0 9, 23 -- Content-Type: text/plain; charset=US-ASCII 9, 23 -- Content-Disposition: inline 9, 23 -- Content-Transfer-Encoding: 8bit 9, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w93GXkrb014540 ==============================End of - Headers==============================
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Email: nicholas.ritchie-at-nist.gov Name: Nicholas Ritchie
Organization: NIST
Title-Subject: [Filtered] New video tutorials for DTSA-II
Message: Over the years, many people have asked about video tutorials for NIST DTSA-II. NIST DTSA-II provides software for quantification and simulation of electron-generated energy dispersive x-ray spectra. It is used all over the world in both the laboratory and class-room environments.
Recently, in response to the requests, we've created half-a-dozen videos and published them on YouTube. The YouTube channel is here: https://www.youtube.com/channel/UCt4nKyhfFQ8xecHyuTnCvIA
(Or search for DTSA-II on YouTube)
Currently, the videos cover an introduction to DTSA-II, quantification, simulation and a couple of other topics. Subscribe to the channel and you will be notified when new videos are published.
Hopefully, you find the videos helpful.
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Email: mike.toalson-at-elementpi.com Name: Mike Toalson
Organization: element Pi, LLC
Title-Subject: [Filtered] Sample Prep Advice for Thin Film
Message: Hoping to get some advice on sample prep for using SEM to measure CVD film thickness via cross section.
Film is sputtered Aluminum with nominal 1 micron thickness on a fluoropolymer substrate using approx 20-40kX magnification.
The aluminum film is too brittle for a razor cut and flakes off or doesn't present a clean edge for measurement. We have tried with some success encapsulating with epoxy support layer before cutting. Also using LN2 for freeze fracture.
Any suggestions how to prepare and mount sample would be much appreciated!!
Mike Toalson element Pi
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Typically for SEM, it is not the section we go for, but the block itself. The ultramicrotome would be used on the embedded block to “polish the surface”. This means taking as small as 60nm slices, gently on a small surface, say 1/2 mm, using a diamond knife and sectioning out onto a water boat on the knife
Possible if the aluminum is very very thin. If it will separate from the block at all with a razor, then using an old used diamond is worth a try first. Usually the trim by hand step with the feel of it under my fingers is how I can tell. The flaking may be thickness dependent, does it “eat” the razor blade? If it eats the blade, it will harm the diamond.
If that does not work, someone more experienced with grinding techniques may have some advice.
This can be put into a holder that grabs the block, or some may epoxy to a stub. If any gluing is done, it needs to pump down in vacuum for 2-3 days before putting it to any SEM
Lou Ann Miller MRL University of Illinois
Sent from my iPad
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We have tried with some } success encapsulating with epoxy support layer before cutting. Also } using LN2 for freeze fracture. } } Any suggestions how to prepare and mount sample would be much appreciated!! } } Mike Toalson } element Pi } } Login Host: 47.208.173.229 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com Fri Oct 5 01:02:40 2018 } 19, 53 -- Received: from mail-qt1-f171.google.com (mail-qt1-f171.google.com [209.85.160.171]) } 19, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w9562ehu031465 } 19, 53 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2018 01:02:40 -0500 } 19, 53 -- Received: by mail-qt1-f171.google.com with SMTP id e22-v6so5589903qto.6 } 19, 53 -- for {microscopy-at-microscopy.com} ; Thu, 04 Oct 2018 23:03:47 -0700 (PDT) } 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=gmail.com; s=20161025; } 19, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 19, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 19, 53 -- bh=26NIynY9J70gXH7X4Jl5+YP8ZO8E8tUC/7PILUkIwRk=; } 19, 53 -- b=ti52fzsAna62wbpTSQNz776i9oAk2GBwluwt4+pGwVchrnCqwepipSiP/q/bmkv2RH } 19, 53 -- HmP56lf0+eq1JUarq2TuZsvSQHK6UFAz8L3fjXGEpS7bGADgqMpQVQ4ki3IGsAY1xq0f } 19, 53 -- qFc7mw/9dhz7oFaglK1Czm4ZNQNIX/ojEEifugJJCRFM/ZfOnWF3JL9SS35pCjeTKmwY } 19, 53 -- HcDcdWn1llpwLHCOzXCeLDDw8c0zhHhKqxNsn/s/Z5RI3p4mq8t3M4zhEHWNP2FhcDFz } 19, 53 -- DvPoBewksadAA5ORrd3d+HXuLOP3oa2Sw8V4ikniQp4/DGBK7QWdqQ/XiEretvBDgEgO } 19, 53 -- Yh7Q== } 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=1e100.net; s=20161025; } 19, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 19, 53 -- :user-agent:mime-version:in-reply-to:content-language } 19, 53 -- :content-transfer-encoding; } 19, 53 -- bh=26NIynY9J70gXH7X4Jl5+YP8ZO8E8tUC/7PILUkIwRk=; } 19, 53 -- b=NRTStRS1K7+JQeo43RKNbSXfW04FF4cw2iIBOKZ0fqgVrrsregGCxQqwrc7l5hd1UU } 19, 53 -- e9pjJPfSjN7yl8qXfirfvGoBdLxkqcM2wiO2L7kXseka+A+53SMMG+LiLuw2Q0M4r+5L } 19, 53 -- PwsVTR5qv6eZb01poQIixhrHLDalk+aXCPwWWWz3bco12qwMqUZ4o+o4G+QtiloS26SZ } 19, 53 -- 7q/uVFCtVdORdGBqNQbxcHQFnatNVdDDvnNP2g7azosV30twYwnCwlNVdFkdLa8vT4p6 } 19, 53 -- x7Ii89AtDRHOgZBPcByJ/hvbJsLjq8HOa16lxnE/aeofSV8HRZKGM+UtMDLU6oXHP9iR } 19, 53 -- AfAQ== } 19, 53 -- X-Gm-Message-State: ABuFfojIDAK59yBWCSxQg2jJhFHyyxWjS2Vh57evjroytZz7Q+d/rlAe } 19, 53 -- kIuQLOHWWRdDmHP0YmO68z8zytp3 } 19, 53 -- X-Google-Smtp-Source: ACcGV63efrh6NgJebku08xIiHPxdd3eALeu+PLgDeZ/1Zvh450Gu5/78RsU9u+xU0/PNUwwvbI3SBA== } 19, 53 -- X-Received: by 2002:ac8:3059:: with SMTP id g25-v6mr8280888qte.136.1538719427025; } 19, 53 -- Thu, 04 Oct 2018 23:03:47 -0700 (PDT) } 19, 53 -- Received: from NestorsnOSX1163.lan (adsl-196.37.6.249.tellas.gr. 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Email: robert.morris-at-unnpp.gov Name: Robert Morris
Organization: Naval Nuclear Laboratory (NNL)
Title-Subject: [Filtered] Career Opportunity: Transmission Electron Microscopy (TEM) Expert
Message: Naval Nuclear Laboratory(NNL) - New York, currently has an opening for a highly skilled Transmission Electron Microscope (TEM) Scientist. At the NNL, we develop advanced naval nuclear propulsion technology, ensuring the safety and reliability of our Navy's submarine and aircraft carrier fleets. This individual will work in an advanced materials characterization laboratory and be responsible for operating and maintaining a FEI Tecnai TEM (new TEM to be procured in 2020). Analysis work: failure analysis investigations, characterization of manufacturing induced features, analyses of material microstructures and chemistries to understand the fundamentals of corrosion and cracking mechanisms.
Microscopy work: high resolution imaging, energy dispersive spectroscopy, electron energy loss spectroscopy, and crystallographic analyses.
Collaboration work: team with other colleagues to conducting sample preparation using Focused Ion Beam (FIB), electropolishing, laser ablation, and ion milling techniques. For more information, please find the posting at: https://navalnuclearlab.energy.gov/jobsearch/job-details/transmission-electron-microscopy-tem-expert/21840/1/
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X-from: Christopher Winkler {microwink-at-gmail.com}
Hello Mike,
Two different ion beam polishing techniques should work for a sample like this, assuming you have access to such equipment. The first and easiest thing to do would be to cut a cross section view using a dual beam (FIB). We've done samples like this before and they turn out well, especially if you're only interested in the Al layer thickness. Another way to prepare a decent cross section is to use a broad beam ion polisher, though you may need to use a cryostage to keep the fluoropolymer substrate from charring and causing the Al layer to delaminate. Both the FIB and broad beam polisher would avoid any possibility of smearing and delamination caused by microtomy and conventional polishing. If you don't have access to such equipment then I'm sure you can locate a lab nearby that does.
Good luck, Chris
On Fri, Oct 5, 2018 at 2:07 AM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } } X-from: mike.toalson-at-elementpi.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both mike.toalson-at-elementpi.com, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: mike.toalson-at-elementpi.com Name: Mike Toalson } } Organization: element Pi, LLC } } Title-Subject: [Filtered] Sample Prep Advice for Thin Film } } Message: Hoping to get some advice on sample prep for using SEM to } measure CVD film thickness via cross section. } } Film is sputtered Aluminum with nominal 1 micron thickness on a } fluoropolymer substrate using approx 20-40kX magnification. } } The aluminum film is too brittle for a razor cut and flakes off or } doesn't present a clean edge for measurement. We have tried with some } success encapsulating with epoxy support layer before cutting. Also } using LN2 for freeze fracture. } } Any suggestions how to prepare and mount sample would be much appreciated!! } } Mike Toalson } element Pi } } Login Host: 47.208.173.229 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 19, 53 -- From microscopy.listserver-at-gmail.com Fri Oct 5 01:02:40 2018 } 19, 53 -- Received: from mail-qt1-f171.google.com (mail-qt1-f171.google.com [209.85.160.171]) } 19, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w9562ehu031465 } 19, 53 -- for {microscopy-at-microscopy.com} ; Fri, 5 Oct 2018 01:02:40 -0500 } 19, 53 -- Received: by mail-qt1-f171.google.com with SMTP id e22-v6so5589903qto.6 } 19, 53 -- for {microscopy-at-microscopy.com} ; Thu, 04 Oct 2018 23:03:47 -0700 (PDT) } 19, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=gmail.com; s=20161025; } 19, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 19, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 19, 53 -- bh=26NIynY9J70gXH7X4Jl5+YP8ZO8E8tUC/7PILUkIwRk=; } 19, 53 -- b=ti52fzsAna62wbpTSQNz776i9oAk2GBwluwt4+pGwVchrnCqwepipSiP/q/bmkv2RH } 19, 53 -- HmP56lf0+eq1JUarq2TuZsvSQHK6UFAz8L3fjXGEpS7bGADgqMpQVQ4ki3IGsAY1xq0f } 19, 53 -- qFc7mw/9dhz7oFaglK1Czm4ZNQNIX/ojEEifugJJCRFM/ZfOnWF3JL9SS35pCjeTKmwY } 19, 53 -- HcDcdWn1llpwLHCOzXCeLDDw8c0zhHhKqxNsn/s/Z5RI3p4mq8t3M4zhEHWNP2FhcDFz } 19, 53 -- DvPoBewksadAA5ORrd3d+HXuLOP3oa2Sw8V4ikniQp4/DGBK7QWdqQ/XiEretvBDgEgO } 19, 53 -- Yh7Q== } 19, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 19, 53 -- d=1e100.net; s=20161025; } 19, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 19, 53 -- :user-agent:mime-version:in-reply-to:content-language } 19, 53 -- :content-transfer-encoding; } 19, 53 -- bh=26NIynY9J70gXH7X4Jl5+YP8ZO8E8tUC/7PILUkIwRk=; } 19, 53 -- b=NRTStRS1K7+JQeo43RKNbSXfW04FF4cw2iIBOKZ0fqgVrrsregGCxQqwrc7l5hd1UU } 19, 53 -- e9pjJPfSjN7yl8qXfirfvGoBdLxkqcM2wiO2L7kXseka+A+53SMMG+LiLuw2Q0M4r+5L } 19, 53 -- PwsVTR5qv6eZb01poQIixhrHLDalk+aXCPwWWWz3bco12qwMqUZ4o+o4G+QtiloS26SZ } 19, 53 -- 7q/uVFCtVdORdGBqNQbxcHQFnatNVdDDvnNP2g7azosV30twYwnCwlNVdFkdLa8vT4p6 } 19, 53 -- x7Ii89AtDRHOgZBPcByJ/hvbJsLjq8HOa16lxnE/aeofSV8HRZKGM+UtMDLU6oXHP9iR } 19, 53 -- AfAQ== } 19, 53 -- X-Gm-Message-State: ABuFfojIDAK59yBWCSxQg2jJhFHyyxWjS2Vh57evjroytZz7Q+d/rlAe } 19, 53 -- kIuQLOHWWRdDmHP0YmO68z8zytp3 } 19, 53 -- X-Google-Smtp-Source: ACcGV63efrh6NgJebku08xIiHPxdd3eALeu+PLgDeZ/1Zvh450Gu5/78RsU9u+xU0/PNUwwvbI3SBA== } 19, 53 -- X-Received: by 2002:ac8:3059:: with SMTP id g25-v6mr8280888qte.136.1538719427025; } 19, 53 -- Thu, 04 Oct 2018 23:03:47 -0700 (PDT) } 19, 53 -- Received: from NestorsnOSX1163.lan (adsl-196.37.6.249.tellas.gr. [37.6.249.196]) } 19, 53 -- by smtp.googlemail.com with ESMTPSA id r79-v6sm3542444qke.31.2018.10.04.23.03.45 } 19, 53 -- for {microscopy-at-microscopy.com} } 19, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 19, 53 -- Thu, 04 Oct 2018 23:03:46 -0700 (PDT) } 19, 53 -- Subject: viaWWW:Sample Prep Advice for Thin Film } 19, 53 -- References: {201810041832.w94IWuBJ007164-at-microscopy.com} } 19, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 19, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 19, 53 -- X-Forwarded-Message-Id: {201810041832.w94IWuBJ007164-at-microscopy.com} } 19, 53 -- Message-ID: {07e3d20f-884c-3713-35bd-aefaa55fb93d-at-gmail.com} } 19, 53 -- Date: Fri, 5 Oct 2018 09:03:42 +0300 } 19, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:52.0) } 19, 53 -- Gecko/20100101 Thunderbird/52.9.1 } 19, 53 -- MIME-Version: 1.0 } 19, 53 -- In-Reply-To: {201810041832.w94IWuBJ007164-at-microscopy.com} } 19, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 19, 53 -- Content-Language: en-US } 19, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
The department of Materials Engineering at UBC's Point Grey campus in Vancouver, BC has an opening for someone to run their electron microscopy lab. The posting can be found here: https://www.hr.ubc.ca/careers-postings/staff.php as job posting 31497.
The short summary is as follows:
Engineering Technician IV This position supports research and teaching activities in Materials Engineering. The position is primarily responsible for the operation and maintenance of the Department's Microscopy Laboratory including the polishing lab and one SEM in the AMPEL facility. The Microscopy Laboratory equipment includes optical and scanning electron microscopes, polishing equipment and X-Ray diffraction equipment. Technician is responsible for instructing and training users (both academic and commercial clients) in safe operation of the equipment. This position supports research and teaching labs when needed. Works with students and research staff to assist in developing experimental and testing procedures. Conducts minor maintenance and repairs. Member of the department Safety committee.
--
Jacob Kabel Director, Electron-Microbeam/X-Ray Diffraction Facility Department of Earth, Ocean & Atmospheric Sciences 6339 Stores Road The University of British Columbia Vancouver, BC, Canada V6T 1Z4
==============================Original Headers============================== 7, 34 -- From jacob.kabel-at-ubc.ca Tue Oct 9 11:30:29 2018 7, 34 -- Received: from vmaprod2.mail-relay.ubc.ca (vmaprod2.mail-relay.ubc.ca [142.103.117.133]) 7, 34 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w99GUTRP009638 7, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 11:30:29 -0500 7, 34 -- Received: from vmaprod2.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 7, 34 -- by localhost (Email Security Appliance) with SMTP id E470962D037_BBCD7F6B 7, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 16:31:50 +0000 (GMT) 7, 34 -- Received: from mx3.mail-relay.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 7, 34 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 7, 34 -- (Client CN "mx3.mail-relay.ubc.ca", Issuer "mx3.mail-relay.ubc.ca" (not verified)) 7, 34 -- by vmaprod2.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id 9A65462A622_BBCD7F6F 7, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 16:31:50 +0000 (GMT) 7, 34 -- Received: from smtp.mail.ubc.ca (s-itsv-hub04p.ead.ubc.ca [137.82.151.86]) 7, 34 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 7, 34 -- (Client CN "rpc.mail.ubc.ca", Issuer "Entrust Certification Authority - L1K" (not verified)) 7, 34 -- by mx3.mail-relay.ubc.ca (Postfix) with ESMTPS id 8AEF216015D 7, 34 -- for {Microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 09:31:50 -0700 (PDT) 7, 34 -- Received: from [137.82.22.226] (10.19.170.34) by smtp.mail.ubc.ca 7, 34 -- (137.82.151.86) with Microsoft SMTP Server id 14.3.399.0; Tue, 9 Oct 2018 7, 34 -- 09:31:49 -0700 7, 34 -- To: {Microscopy-at-microscopy.com} 7, 34 -- From: Jacob Kabel {jacob.kabel-at-ubc.ca} 7, 34 -- Subject: Job Posting - UBC Vancouver 7, 34 -- Message-ID: {ca24500c-d9f8-cbf5-5bf7-0f73a1cb88fd-at-ubc.ca} 7, 34 -- Date: Tue, 9 Oct 2018 09:31:45 -0700 7, 34 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:60.0) Gecko/20100101 7, 34 -- Thunderbird/60.2.1 7, 34 -- MIME-Version: 1.0 7, 34 -- Content-Type: text/plain; charset="utf-8"; format=flowed 7, 34 -- Content-Transfer-Encoding: 8bit 7, 34 -- Content-Language: en-US 7, 34 -- X-Originating-IP: [10.19.170.34] 7, 34 -- X-PMWin-Version: 3.1.3.0, Antivirus-Engine: 3.73.0, Antivirus-Data: 5.55 7, 34 -- X-SASI-RCODE: 200 ==============================End of - Headers==============================
Just throwing up this job opening left by my colleague who moved to a different lab/department. I am not associated with or responsible for this listing, so please do not contact me to inquire about it.
This position supports research and teaching activities in Materials Engineering. The position is primarily responsible for the operation and maintenance of the Department's Microscopy Laboratory including the polishing lab and one SEM in the AMPEL facility. The Microscopy Laboratory equipment includes optical and scanning electron microscopes, polishing equipment and X-Ray diffraction equipment. Technician is responsible for instructing and training users (both academic and commercial clients) in safe operation of the equipment.
This position supports research and teaching labs when needed. Works with students and research staff to assist in developing experimental and testing procedures. Conducts minor maintenance and repairs. Member of the department Safety committee.
Cheers, Bradford Ross
Electron Microscopy Technician BioImaging Facility University of British Columbia Cunningham Building Rm. 64 2146 East Mall Vancouver, B.C. V6T 1Z4
phone 604-822-6996
==============================Original Headers============================== 17, 38 -- From bradford.ross-at-botany.ubc.ca Tue Oct 9 15:56:29 2018 17, 38 -- Received: from vmaprod6.mail-relay.ubc.ca (vmaprod6.mail-relay.ubc.ca [142.103.117.142]) 17, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w99KuTqc022602 17, 38 -- for {microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 15:56:29 -0500 17, 38 -- Received: from vmaprod6.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 17, 38 -- by localhost (Email Security Appliance) with SMTP id 264E62A6DA8_BBD164FB 17, 38 -- for {microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 20:57:51 +0000 (GMT) 17, 38 -- Received: from mx1.mail-relay.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 17, 38 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 17, 38 -- (Client CN "mx1.mail-relay.ubc.ca", Issuer "mx1.mail-relay.ubc.ca" (not verified)) 17, 38 -- by vmaprod6.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id CDB8B2A4114_BBD164EF 17, 38 -- for {microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 20:57:50 +0000 (GMT) 17, 38 -- Received: from smtp.mail.ubc.ca (s-itsv-hub03p.ead.ubc.ca [137.82.151.73]) 17, 38 -- (using TLSv1 with cipher DHE-RSA-AES256-SHA (256/256 bits)) 17, 38 -- (Client CN "rpc.mail.ubc.ca", Issuer "Entrust Certification Authority - L1K" (not verified)) 17, 38 -- by mx1.mail-relay.ubc.ca (Postfix) with ESMTPS id C0E6915FAE9 17, 38 -- for {microscopy-at-microscopy.com} ; Tue, 9 Oct 2018 13:57:50 -0700 (PDT) 17, 38 -- Received: from S-ITSV-MBX05P.ead.ubc.ca ([169.254.12.143]) by 17, 38 -- s-itsv-hub03p.ead.ubc.ca ([137.82.151.73]) with mapi id 14.03.0399.000; Tue, 17, 38 -- 9 Oct 2018 13:57:50 -0700 17, 38 -- From: "Ross, Bradford" {bradford.ross-at-botany.ubc.ca} 17, 38 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 17, 38 -- Subject: Materials EM Tech Position at UBC 17, 38 -- Thread-Topic: Materials EM Tech Position at UBC 17, 38 -- Thread-Index: AdRgEr1Gvq6romoKTyeZjJIPQuKwzg== 17, 38 -- Date: Tue, 9 Oct 2018 20:57:50 +0000 17, 38 -- Message-ID: {B48A2601A4664A4AAF5A3D2F0E482801025E60878B-at-s-itsv-mbx05p.ead.ubc.ca} 17, 38 -- Accept-Language: en-US, en-CA 17, 38 -- Content-Language: en-US 17, 38 -- X-MS-Has-Attach: 17, 38 -- X-MS-TNEF-Correlator: 17, 38 -- x-originating-ip: [10.19.216.46] 17, 38 -- x-pmwin-version: 3.1.3.0, Antivirus-Engine: 3.73.0, Antivirus-Data: 5.55 17, 38 -- Content-Type: text/plain; charset="us-ascii" 17, 38 -- MIME-Version: 1.0 17, 38 -- X-SASI-RCODE: 200 17, 38 -- Content-Transfer-Encoding: 8bit 17, 38 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w99KuTqc022602 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both eric.leroy-at-icmpe.cnrs.fr, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy
Organization: CNRS
Title-Subject: [Filtered] Tensile sample holder for tecnai
Message: Hi, I am looking for a tensile sample holder for a Tecnai F20 equipped with a S-Twin pole piece. So far I only found Gatan as manufacturer. Do you know other manufacturers? Thank you for your help Best regards,
Eric LEROY Directeur de la plateforme microscopie lectronique ICMPE - UMR 7182 2/8, rue Henri Dunant 94320 THIAIS T : 01.49.78.12.09 F : 01.49.78.12.03
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-------- Forwarded Message -------- X-from: Chris Jones {C.Jones-at-nhm.ac.uk}
Have you tried Deben in the UK? They manufacture many stages, attachments, etc for electron beam instruments.
Good luck!
Chris
------------------------------------------------------------------------ *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* 12 October 2018 16:48 *To:* Chris Jones *Subject:* [Microscopy] viaWWW: Tensile sample holder for tecnai
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X-from: eric.leroy-at-icmpe.cnrs.fr
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both eric.leroy-at-icmpe.cnrs.fr, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy
Organization: CNRS
Title-Subject: [Filtered] Tensile sample holder for tecnai
Message: Hi, I am looking for a tensile sample holder for a Tecnai F20 equipped with a S-Twin pole piece. So far I only found Gatan as manufacturer. Do you know other manufacturers? Thank you for your help Best regards,
Eric LEROY Directeur de la plateforme microscopie lectronique ICMPE - UMR 7182 2/8, rue Henri Dunant 94320 THIAIS T : 01.49.78.12.09 F : 01.49.78.12.03
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both weutro-at-yahoo.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: weutro-at-yahoo.com Name: GORDON
Title-Subject: [Filtered] Charging in FIB
Message: I need some advice on sample prep for FIB. I have packaged parts, with gold bond wires, that goes into a FIB to do circuit edits. Due to the samples being in a package, the samples would charge. If I coat the sample, it would help the charging issue but then I need to remove the coat after I'm done with the FIB edits. Does anyone have any suggestions on what to coat it with and how to remove the coat afterwards? Thanks Gordon
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both rcsencsits-at-belcan.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: rcsencsits-at-belcan.com
Name: Roseann Csencsits
Organization: Belcan Vallecitos Laboratories
Title-Subject: [Filtered] Northern California Society for Microscopy Fall Meeting Nov 8th in Pleasanton, CA
Message: Join us for lunch, lectures and socializing, Thursday November 8.
RSVP via email to info-at-ncsmicroscopy.org by October 15.
11:30 - 11:45 am Check-in; demo sign up 11:45 - 12:00 pm Lunch, seating for lectures 12:00 - 2:00 pm Lectures De Wood, USDA, Agricultural Applications of Microscopy
Justin Ondry, UC Berkeley,"Understanding the Removal Pathways of Dislocations in Imperfectly Attached Nanocrystals using in-situ HRTEM Matt Hauwiller, UC Berkeley, Utilizing Graphene Liquid Cell TEM to Elucidate the Mechanisms ofNon-Equilibrium Etching of Metallic Nanocrystals
Ray Twesten, Gatan, Advanced EELS acquisition and analysis
2:00-2:10 pm NCSM Bylaws Discussion
2:10-3:30 pm Coffee, snacks, conversation, tour/demos of Gatans EM labs
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Email: lavoie-at-uw.edu Name: Ellen Lavoie
Organization: University of Washington
Title-Subject: [Filtered] OCT embedded sample prep
Message: Hello Everyone,
I have an interesting one here...I'd like to take a previously unfixed (mouse) tissue sample that has been frozen in OCT and get it to the point of being embedded in epoxy for TEM. Yes, I know it can be sectioned with cryomicrotome then imaged in cryo but that's not an option right now. My thought involves HPF but not sure how to transition it without dealing with a big mess. Thoughts? If so please message me at lavoie-at-uw.edu
Cheers, Ellen Lavoie UW MAF Seattle, WA
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Email: zhuoli-at-coh.org Name: Zhuo Li
Organization: City of Hope
Title-Subject: [Filtered] SCSMM 2018 fall meeting Nov 14, 2018
Message: You are welcome to come to the Southern California Society for Microscopy and Microanalysis (SCSMM) 2018 fall meeting on Wednesday, November 14th (one week before Thanksgiving) at City of Hope Beckman Center Argyros Auditorium. The invited speakers are Dr. Elena Mirada from Cal State Northridge, and Dr. Jacob Berlin from City of Hope Beckman Research Institute. In order to register, please sign up on-line using the link (https://imri.uci.edu/content/2018-fall-meeting-registration#overlay-context=content/2018-fall-meeting-registration) no later than 5 p.m. Friday, November 9th. On-line registration is required.
CN Tech based in Cambridgeshire UK, manufactures and distributes Swift Tensile stages in Europe. We also have representatives around the world, which include Electron Microscopy Sciences in the USA.
We specialise in customisable tensile stages to fit any microscope with or without heating and chilling.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 13 October 2018 13:05 To: Jon Nottingham {jon-at-cntech.co.uk}
-------- Forwarded Message -------- X-from: Chris Jones {C.Jones-at-nhm.ac.uk}
Have you tried Deben in the UK? They manufacture many stages, attachments, etc for electron beam instruments.
Good luck!
Chris
------------------------------------------------------------------------ *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* 12 October 2018 16:48 *To:* Chris Jones *Subject:* [Microscopy] viaWWW: Tensile sample holder for tecnai
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X-from: eric.leroy-at-icmpe.cnrs.fr
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Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy
Organization: CNRS
Title-Subject: [Filtered] Tensile sample holder for tecnai
Message: Hi, I am looking for a tensile sample holder for a Tecnai F20 equipped with a S-Twin pole piece. So far I only found Gatan as manufacturer. Do you know other manufacturers? Thank you for your help Best regards,
Eric LEROY Directeur de la plateforme microscopie lectronique ICMPE - UMR 7182 2/8, rue Henri Dunant 94320 THIAIS T : 01.49.78.12.09 F : 01.49.78.12.03
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There are two days (October 17 deadline) remaining for registration for the workshop “Atom by atom fabrication via electron beams and scanning probes”, to be held at CNMS, Oak Ridge National Laboratory on November 1,2. This workshop will bring together experts in scanning probe atomic fabrication and emerging field of atomically resolved electron beam matter manipulation, to highlight the recent advances and opportunities and serve as a much-needed seed to establish its rapid growth. It will provide a forum to present recent achievements in electron beam manipulation, novel opportunities for instrumental development enabled by the availability of high speed data analytic tools and machine learning, integration of atomic-scale device engineering into semiconductor workflows, and opportunities for quantum information systems.
The confirmed plenary and invited speakers include Toma Susi (U. Vienna) and John Randall (Zyvex), as well as David Forrest (DOE), and Khershed Cooper (NSF). A number of participants from semiconductor and quantum computing industry and DARPA are also participating, so the workshop can offer great opportunities for networking. The partial program (allowing for additional invited and regular contributions based on submissions) is provided below.
*9:15 – 10:00 *T. Susi (U. Vienna), /Electron-beam manipulation of graphene impurities: competing processes and modeling challenges/
*10.00 – 10.30*David Forrest (DOE EERE), /Atomically Precise Manufacturing for Energy Applications/
*10:30 - 11:00*/Break/
*11:00 - 11:30*A.L. Bleloch (Nion), /Precision control of the electron beam and the sample environment in the STEM/
*11:30 – 12:00*Rick Silver (NIST), /Robust Fabrication and Measurement of Atomically Precise Devices/
*12:00 – 12:15*K. Cooper (NSF), /NSF Nano and Advanced Manufacturing Research at NSF/
*12:15 – 12:30*(slot)
*12:30 - 13:30*/Lunch/
*13:30 - 14:00*Stephen Jesse, /Scanning Transmission Electron Microscopy for Atomic Manipulation: strategies for in-line image and spectral analysis for feedback and controls/
X-from: Valery Ray {webmaster-at-partbeamsystech.com}
Hi Gordon,
If you set proper GAE processes and use primary ion beam currents appropriate for edits, then there wouldn't be any problems with surface charging or ESD damage.
If there is no time/bandwidth/money/expertise to accomplish the above, then there are two surface coating options suitable for brute-force elimination of surface charging during FIB circuit edit: (a) carbon, either evaporated or PIPS, or conductive polymers, either spin-coated or ultrasonic-nozzle dispensed. Carbon deposited with thickness of about 20nm provides sufficient charge dissipation for CE-appropriate beam currents while having little to no influence on regular operation of most ICs, so it can be left on in most cases. If removal is required, then O2 plasma cleaner ( {20W), or ozone asher, or UV cleaner would work (from fastest to slowest) depending on sensitivity of the device. Conductive polymers simply washed away after the edit site has been capped an sealed.
Google for "Free of charge FIB circuit edit of ICs" and "New FIB tricks with old conductive polymers"
Happy editing! Valery
P.S. I am assuming that all pins of device under edit already have solid connection to the stage of your CE FIB.
Valery Ray Also with REFINE Center, UCONN ============================== PBS&T, MEO Engineering Company 290 Broadway, Suite 298 Methuen, MA 01844, USA Phone: +1-978-305-0479 (leave a message) E-mail: vray-at-partbeamsystech.com Web: www.partbeamsystech.com Web: www.freudlabs.com
On 10/14/2018 10:38 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } } -------- Forwarded Message -------- } } X-from: weutro-at-yahoo.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both weutro-at-yahoo.com, Microscopy-at-Microscopy.com } so that all Microscopy Listserver Subscribers can benefit from our } collective wisdom } --------------------------------------------------------------------------- } } Email: weutro-at-yahoo.com Name: GORDON } } Title-Subject: [Filtered] Charging in FIB } } Message: I need some advice on sample prep for FIB. } I have packaged parts, with gold bond wires, that goes into a FIB to do } circuit edits. Due to the samples being in a package, the samples would } charge. If I coat the sample, it would help the charging issue but then } I need to remove the coat after I'm done with the FIB edits. Does anyone } have any suggestions on what to coat it with and how to remove the coat } afterwards? } Thanks } Gordon } } Login Host: 198.175.253.81 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 12, 53 -- From microscopy.listserver-at-gmail.com Sun Oct 14 21:37:37 2018 } 12, 53 -- Received: from mail-wr1-f41.google.com (mail-wr1-f41.google.com [209.85.221.41]) } 12, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w9F2bbqw003399 } 12, 53 -- for {microscopy-at-microscopy.com} ; Sun, 14 Oct 2018 21:37:37 -0500 } 12, 53 -- Received: by mail-wr1-f41.google.com with SMTP id d2-v6so19421626wro.7 } 12, 53 -- for {microscopy-at-microscopy.com} ; Sun, 14 Oct 2018 19:39:16 -0700 (PDT) } 12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=gmail.com; s=20161025; } 12, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 53 -- bh=0zd/yfj9Z5mTiZB7di56KJ8P9uhrRf3BzgmjHo/sK30=; } 12, 53 -- b=tgLwwbUXnqiqCcqgSLeIfPrBOK2zJ3ta3lBVZA0oXpedpXlQ7tnl1KIt36ygIPO5+N } 12, 53 -- xnG50G6YPdKBAkb1YwvV+9aw3RIvWFEiuthqxAFqLdD4JsVYloXSPupXTV0nDhjMBF8L } 12, 53 -- kbkzpX3oat4s+x0+b2g9qC758JmVJ0h/VhrQF6i6cYP9UBiJ5z1rxuO2IpvJvldAqc63 } 12, 53 -- q9Av5edhDiE4JITGuNqihRCw9rA53+oEPJn+aMt5ijJxa20Wip4uy7+cAum+tHMF2MpD } 12, 53 -- h09R87+kM0A2wTWe4lK4CMMrZiFX49iQ1NWp7JETXvwwi6Ja+k5FXKuV4x00U/fcSBgO } 12, 53 -- cO2Q== } 12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=1e100.net; s=20161025; } 12, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 53 -- :user-agent:mime-version:in-reply-to:content-language } 12, 53 -- :content-transfer-encoding; } 12, 53 -- bh=0zd/yfj9Z5mTiZB7di56KJ8P9uhrRf3BzgmjHo/sK30=; } 12, 53 -- b=MUiy6U2duZT35Agqxm1jtKv4y0Z/zjG6Qyr14ApQzflVCxQcTgqKU8DsVJ4fu86Lo2 } 12, 53 -- lOi61hWKwFTI9rn0bmhWUMvEd3sXdsGRDSwufBt1Hv9L6EhO8HJTKkBQcgmH+1qn4jYj } 12, 53 -- Gw/Sr9Ih6EZNXSw08Ee3U+jzoj/VWKeudhxiudaj22JXPIeZlTNuN4rxeRSd7II6GGxI } 12, 53 -- GU4acvqlVwJKeZAyWLyV1UVmQO8u4v9fac8Y8YHm29S1jdpJpfqTQ1Dn19OxRG7VcD9G } 12, 53 -- TVPaKIpWj+uNC3WFLKCGklpnVZwBOvN7ApKSusTJNUvootPOwgZsGucdrs0VNICDVoEM } 12, 53 -- sAyw== } 12, 53 -- X-Gm-Message-State: ABuFfoiipWYw5H9kf7+TU6CSLN5vmq7rP6GtCujtOOORpYwdDQVe4Tdv } 12, 53 -- OIQVMLZ1KiLW+bTeQxgPaWxF+QcH } 12, 53 -- X-Google-Smtp-Source: ACcGV62JyRZNsbjWq2Ci0eE58aGss3zzpZtQ+Yj2czNsUReEyB5VFnAKdXQ8LfR8c/irwPKLq7oDHg== } 12, 53 -- X-Received: by 2002:adf:cd0c:: with SMTP id w12-v6mr13268592wrm.67.1539571155840; } 12, 53 -- Sun, 14 Oct 2018 19:39:15 -0700 (PDT) } 12, 53 -- Received: from Nestors-MacBookAir-Pro-2014-ElCapitan-OSX-1163.local ([62.214.191.20]) } 12, 53 -- by smtp.googlemail.com with ESMTPSA id t3-v6sm7476905wru.47.2018.10.14.19.39.14 } 12, 53 -- for {microscopy-at-microscopy.com} } 12, 53 -- (version=TLS1_2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128/128); } 12, 53 -- Sun, 14 Oct 2018 19:39:15 -0700 (PDT) } 12, 53 -- Subject: viaWWW: Charging in FIB } 12, 53 -- References: {201810121611.w9CGBQuf011828-at-microscopy.com} } 12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 53 -- From: Microscopy Listserver {microscopy.listserver-at-gmail.com} } 12, 53 -- X-Forwarded-Message-Id: {201810121611.w9CGBQuf011828-at-microscopy.com} } 12, 53 -- Message-ID: {3984c861-df9a-5ad9-60c4-b9fc32e4c0f8-at-gmail.com} } 12, 53 -- Date: Mon, 15 Oct 2018 04:39:14 +0200 } 12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:52.0) } 12, 53 -- Gecko/20100101 Thunderbird/52.9.1 } 12, 53 -- MIME-Version: 1.0 } 12, 53 -- In-Reply-To: {201810121611.w9CGBQuf011828-at-microscopy.com} } 12, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 12, 53 -- Content-Language: en-US } 12, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
X-from: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}
Hi Gordon,
Sorry for the shameless self-promotion, but we actually published a paper a little while back about using conductive polymers (used for e-beam lithography) in the FIB. We were using it on flat wafer samples, but you might be able to adapt the technique we described for your needs. Most of the polymers are water soluble and can be removed with a rinse afterwards. Please let me know if you need a PDF copy of the article.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Sunday, October 14, 2018 22:40 To: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}
Dear colleagues
The STEM group at ORNL has open two postdoctoral research positions:
1. Postdoctoral Research Associate – Electron Microscopy of Quantum Materials / NB50693574 2. Postdoctoral Research Associate - STEM of Heterostructured Quantum Materials / NB50694303
The descriptions are as following:
Electron Microscopy of Quantum Materials: We are seeking a Postdoctoral Research Associate to conduct research on the atomistic origins of quantum phenomena in novel material systems using high-resolution electron microscopy and spectroscopy combined with machine learning and artificial intelligence tools. Examples of relevant physical systems include spin-liquid layered halides, charge-density wave dichalcogenides, and other 2D materials. In this role, you will work closely with the scanning transmission electron microscopy (STEM) team at ORNL to conduct research using high-spatial and energy resolution tools, and you will also collaborate with machine learning experts at ORNL that will include deep learning and simulation efforts on the Summit supercomputer. This position resides in the Electron & Atom Probe Microscopy Group in the Center for Nanophase Materials Sciences (CNMS) Division, Physical Sciences Directorate (PSD) at Oak Ridge National Laboratory (ORNL).
STEM of Heterostructured Quantum Materials: As a Postdoctoral Research Associate, you will conduct research on epitaxial complex-oxide thin films and heterostructures as well as chalcogenide-based quantum materials using scanning transmission electron microscopy/electron energy-loss spectroscopy (STEM/EELS). This position is to lead our ongoing efforts in understanding interfacial phenomena in functional perovskite oxide materials for emerging information and energy technologies as well as the area of quantum materials for quantum information science. The work involves close collaboration across groups to utilize advanced imaging and high-resolution STEM/EELS to study thin film structures prepared by both pulsed laser deposition (PLD) and molecular beam epitaxy (MBE). This position will involve close collaboration with both microscopists and film growers and will be using advanced STEM facilities at ORNL.
Please apply directly via ORNL recruiting website.
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 13, 37 -- From sergei2-at-ornl.gov Tue Oct 16 13:10:02 2018 13, 37 -- Received: from mta01.ornl.gov (mta01.ornl.gov [128.219.177.137]) 13, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w9GIA2Lh023542 13, 37 -- for {microscopy-at-microscopy.com} ; Tue, 16 Oct 2018 13:10:02 -0500 13, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 13, 37 -- t=1539713507; x=1571249507; 13, 37 -- h=to:from:subject:message-id:date:mime-version: 13, 37 -- content-transfer-encoding; 13, 37 -- bh=z90lmJGkgCoQ4/4r3ahky6r8ZTLaEeI5RsIQ+S3UoYA=; 13, 37 -- b=xAqPN16xGKJ1nmY+zRajDffmi4qhVXwaFWXReMUv8V/ZotV78Tnesg0H 13, 37 -- VG6mVFLwQKFcZ3C4gfTXo3rGKBlffp8V221OwukKvwdL/4YVVQEkSNs4+ 13, 37 -- bVUBhXRCZ74+W7iWdRSlSEg9i2Lf8TN8tUwB526rAnNyRRIOa/xSX9q0k 13, 37 -- xx2WSk8y1x4kM83NscM4qQp1vJl9xhN5pPHsAXe0+D7Mprd75B8oyWJCL 13, 37 -- ybMLlmEwPTfvvRbEAv9SWvjuYDYcMLcIMY63OlmwYZIC/LDd/kMOW2dCr 13, 37 -- SiuIJQpl19uKzbQ/SOG+4ZE7SkCMgkGW1PW9e4nXbfHZcsGV8cD5Sq1a/ 13, 37 -- A==; 13, 37 -- X-SG: RELAYLIST 13, 37 -- X-IronPort-AV: E=Sophos;i="5.54,389,1534824000"; 13, 37 -- d="scan'208";a="50051970" 13, 37 -- Received: from emgwy1.ornl.gov ([160.91.254.9]) 13, 37 -- by iron1.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 16 Oct 2018 14:11:47 -0400 13, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 13, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 13, 37 -- (No client certificate requested) 13, 37 -- by emgwy1.ornl.gov (Postfix) with ESMTPS id 42ZNg70swKz2SrWx 13, 37 -- for {microscopy-at-microscopy.com} ; Tue, 16 Oct 2018 14:11:47 -0400 (EDT) 13, 37 -- To: microscopy-at-microscopy.com 13, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 13, 37 -- Subject: 2 Postdoctoral positions in STEM - ORNL 13, 37 -- Message-ID: {5BC629E2.40804-at-ornl.gov} 13, 37 -- Date: Tue, 16 Oct 2018 14:11:46 -0400 13, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 13, 37 -- Thunderbird/38.0.1 13, 37 -- MIME-Version: 1.0 13, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 13, 37 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: samuel.livingston-at-botany.ubc.ca Name: Sam Livingston
Organization: University of British Columbia
Title-Subject: [Filtered] Difficulty obtaining Tokuyasu ultrathin sections of plant material
Message: Dear Microscopy listserv,
I am having difficulty obtaining ultrathin sections of leaves and flowers with the purpose of performing immunohistochemistry. My samples were fixed in 4% PFA in TBS-T, infiltrated in an ascending grade of sucrose up to 2.3 M, then embedded on a sectioning pin surrounded by 2.3 M sucrose by dipping into liquid nitrogen. I am sectioning on an ultramicrotome equipped with a cryobox.
I'm finding that sectioning at 1 um thickness at -65 C has minimized the amount of sucrose flaking, and is providing me with the best sections for all conditions I have tried (from -60 to -90 at 5 C increments; 1.5 um to 0.3 um with .15 um increments). However, I am still experiencing several issues:
The issues I'm having at 1 um sections; -65 C: - sections are wrinkling and curling off the knife edge - tissue sections do not adhere to slides after transferring out of cryobox - tissue is largely fragmented upon imaging with TolBlue staining and transmitted light, where epidermis, mesophyll and epidermal outgrowths are floating all over the slide
My major concerns relate to the sections not adhering to my slides after transfer, which will certainly wash off my slides during the immunolabeling work up, and the fragmentation of my tissue, which removes the spatial context needed for immunolabeling.
I have tried several different types of slides including uncoated, pre-cleaned slides from Fisher, uncoated and non-cleaned slides from VWR, as well as PTFE coated slides from EMS, with no apparent difference in section adherence. If you have any tips, tricks or recommendations please let me know, as I would be very grateful for any help on this technically challenging and patience testing method.
Sam Livingston
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You say you've tried several adhesives but have you tried chrom-alum, which works well in many cases. It's relatively easy to make - there are many different recipes, e.g http://stainsfile.info/StainsFile/prepare/adhesives/chromegelatin.htm. We resorted to this after poly-lysine-, agar- and silane-coated slides - either purchased or prepared ourselves, proved unreliable with a particularly difficult tissue.
I'm guessing the fragmentation of the tissue is because the cell walls are not sufficiently well fixed/penetrated by the various solutions. Does happen with frozen plant tissues. For example, rapid-frozen/freeze-substituted Arabidopsis roots have fantastically-preserved cell contents but the cells tend to separate as you section the resin blocks. Tobias Baskin comments on this in one of his earlier papers, I think.
good luck, cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601
T 61 2 6246 5475 M 61 468 966 713 ________________________________________ X-from: microscopy.listserver-at-gmail.com [microscopy.listserver-at-gmail.com] Sent: Thursday, 18 October 2018 6:21 a.m. To: White, Rosemary (A&F, Black Mountain)
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Email: samuel.livingston-at-botany.ubc.ca Name: Sam Livingston
Organization: University of British Columbia
Title-Subject: [Filtered] Difficulty obtaining Tokuyasu ultrathin sections of plant material
Message: Dear Microscopy listserv,
I am having difficulty obtaining ultrathin sections of leaves and flowers with the purpose of performing immunohistochemistry. My samples were fixed in 4% PFA in TBS-T, infiltrated in an ascending grade of sucrose up to 2.3 M, then embedded on a sectioning pin surrounded by 2.3 M sucrose by dipping into liquid nitrogen. I am sectioning on an ultramicrotome equipped with a cryobox.
I'm finding that sectioning at 1 um thickness at -65 C has minimized the amount of sucrose flaking, and is providing me with the best sections for all conditions I have tried (from -60 to -90 at 5 C increments; 1.5 um to 0.3 um with .15 um increments). However, I am still experiencing several issues:
The issues I'm having at 1 um sections; -65 C: - sections are wrinkling and curling off the knife edge - tissue sections do not adhere to slides after transferring out of cryobox - tissue is largely fragmented upon imaging with TolBlue staining and transmitted light, where epidermis, mesophyll and epidermal outgrowths are floating all over the slide
My major concerns relate to the sections not adhering to my slides after transfer, which will certainly wash off my slides during the immunolabeling work up, and the fragmentation of my tissue, which removes the spatial context needed for immunolabeling.
I have tried several different types of slides including uncoated, pre-cleaned slides from Fisher, uncoated and non-cleaned slides from VWR, as well as PTFE coated slides from EMS, with no apparent difference in section adherence. If you have any tips, tricks or recommendations please let me know, as I would be very grateful for any help on this technically challenging and patience testing method.
Sam Livingston
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Email: zluo-at-uncfsu.edu Name: Zhiping Luo
Organization: Fayetteville State University
Title-Subject: [Filtered] Research Associate position in EPMA/SEM available at Fayetteville State University
Message: A Research Associate regular staff position for EPMA/SEM is immediately available at FSU:
https://jobs.uncfsu.edu/postings/17614.
FSU houses an advanced JEOL JXA-8530F EPMA with a newly installed xCLent cathodoluminescence system. More information can be found at https://www.uncfsu.edu/research/sencr-mic.
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Message: Hello. I am struggling to install new memory onto our Biowave. It won't read any of the thumb drives we have tried. W tried reformatting, but nothing works. Has anyone else had this problem? I am trying to restore the original settling that I erased by mistake. Thanks JoAnn
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Title-Subject: [Filtered] Imaging of pig pericardium
Message: We are looking for a suitable technique to establish the 3D microstructure of a decellularized sheet of swine pericardium (~15cmx8cmx0.5cm), with high enough resolution to visualize the orientation and 3-D arrangement of collagen bundles and detect anomalies or inflection points. The large size of the specimen makes it impractical for uCT. We were thinking of confocal stereomicroscopy using collagen autofluorescence. We welcome practical suggestions. Thank you!
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Email: dlowry-at-asu.edu Name: David Lowry
Organization: Arizona State University
Title-Subject: [Filtered] used/salvage parts for JEOL SEM
Message: I am looking for a source of used parts for a JEOL JSM-6300 or possibly 6400 series SEM, specifically the video boards that control the CRTs. If anyone has a salvage unit or parts, or if anyone knows of a potential parts source, please contact me off-line. thanks- Dave
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Email: thoward-at-unm.edu Name: Tamara Howard
Organization: University of New Mexico
Title-Subject: [Filtered] FE-SEM gun lifetime
Message: I've sort of inherited oversight of a shared EM facility that includes a 2-year old FE-SEM. Unfortunately, the system does not get much use, so I'd like to be able to shut down the gun when there are no projects anticipated - it just costs too much for us to be able to replace the tip every 12-16 months. The original one was swapped out at just under 24 months of age with less than 200 hours of actual use. It was still working & seemed to be stable, but was well over the suggested run time.
I've been told by various people that there are facilities that routinely turn their FE-gun off when not in use, but they did not know if anyone had standardized a re-start procedure. So, my question is: would anyone who does this mind sharing how they manage this? I will see replies to the ListServer; I'm having some issues with Outlook and could not post directly.
Thanks for your help!
Tamara
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Hi Tamara, inheriting other microscopes can be a gift or a burden - who knows. but in this case, a 2-yr old FE-SEM is - usually a wonderful gift. The original gun was swapped out at just under 24 months of age ??? strange. if it is an FE-gun, it can live 'for ever', if not heavily used. - In our FE-SEM (from 1999), we had one for far more than 5 years -- with little / occasional use (before I got it). What a shame for such an instrument. The gun was fine. But, after 200 hrs, an FE-gun is not "well over the suggested run time" -- NO, NEVER. A tungsten filament may be ... but even then: if it still gives nice images, it was treated well, and it can stay there. You write: " it was still working & seemed stable" - then, why changing? (Although, exchanging a W filament is not too tricky, and from the budget, not too heavy). Basically, for all types of filaments, it is the usage AND the vacuum , which limits the lifetime. The level of vacuum which is required, is very different, for the different guns (W, LaB6, FE). IF you really have an FE-SEM, then you may ask the manufacturer for an appropriate treatment during extended periods of "no use". And they also should have a procedure for a proper re-start . They should ... Our FE-SEM and also our FE-TEM, both have one (and yes, for extended periods, I use the shut-down / re-start of the manufacturer). Works fine. - In our W-SEM and W-TEM, filaments are used until burnt. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 Elected member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz
==============================Original Headers============================== 7, 23 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Sun Oct 28 05:49:32 2018 7, 23 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 7, 23 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id w9SAnV64027964 7, 23 -- for {Microscopy-at-microscopy.com} ; Sun, 28 Oct 2018 05:49:32 -0500 7, 23 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 7, 23 -- by localhost (Postfix) with SMTP id 1A6FC5EC08 7, 23 -- for {Microscopy-at-microscopy.com} ; Sun, 28 Oct 2018 11:51:54 +0100 (CET) 7, 23 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 23 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id F01A75EAEC 7, 23 -- for {Microscopy-at-microscopy.com} ; Sun, 28 Oct 2018 11:51:53 +0100 (CET) 7, 23 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 23 -- with Novell_GroupWise; Sun, 28 Oct 2018 11:51:53 +0100 7, 23 -- Message-Id: {5BD594C8020000540007B211-at-gwsmtp1.uni-regensburg.de} 7, 23 -- X-Mailer: Novell GroupWise Internet Agent 18.0.2 7, 23 -- Date: Sun, 28 Oct 2018 11:51:52 +0100 7, 23 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 7, 23 -- To: "microscopy server" {Microscopy-at-microscopy.com} 7, 23 -- Subject: gun lifetime 7, 23 -- Mime-Version: 1.0 7, 23 -- Content-Type: text/plain; charset=US-ASCII 7, 23 -- Content-Disposition: inline 7, 23 -- Content-Transfer-Encoding: 8bit 7, 23 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id w9SAnV64027964 ==============================End of - Headers==============================
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Email: robless-at-usc.edu Name: Susan Robles
Organization: University of Southern California/Center of Excellence in Nano Imaging (CNI)
Title-Subject: [Filtered] Bio-EM Senior Scientist: Electron Microscopist - TEM
Message: University of Southern California, is seeking an outstanding candidate for the above position.
For further information: https://usccareers.usc.edu/job/los-angeles/bio-em-senior-scientist/1209/9747114
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Email: ecdickey-at-ncsu.edu Name: Elizabeth Dickey
Organization: North Carolina State University
Title-Subject: [Filtered] Biological Electron Microscopy Staff Scientist Position
Message: The Analytical Instrumentation Facility (AIF) at NC State University is seeking an expert staff scientist to lead its efforts in biological electron microscopy and associated sample preparation techniques. The AIF is a leading microscopy center in the Southeastern U.S. and is expanding its biological and soft-matter characterization capabilities. To this end, the AIF has recently installed a Talos G2 S/TEM with low-temperature imaging capabilities to complement its current cryo-SEM and other analytical tools. Concurrently, the AIF is working to add other key sample preparation equipment, including freeze fracture and high pressure freezing. The staff scientist will work closely with multiple NC State Departments, including but not limited to Plant and Microbial Biology, Biomedical Engineering, Crop and Soil Sciences, Veterinary Medicine, Mechanical Engineering, and Materials Science and Engineering. The staff scientist will develop workshops and train users on topics of biological sample preparation and imaging through the Research Triangle Nanotechnology Network (RTNN), a site in the National Nanotechnology Coordinated Infrastructure (NNCI). The staff scientist will also spend dedicated time performing collaborative research within the multi-disciplinary Center for Lignocellulose Structure and Formation (www.lignocellulose.org/) under the direction of Dr. Candace Haigler (https://cals.ncsu.edu/plant-and-microbial-biology/people/chhaigle/). Further information and instructions on how to apply can be found at:
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X-from: Pappas, Richard Steve (CDC/DDNID/NCEH) {rlp6-at-cdc.gov} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Hi Tamara, inheriting other microscopes can be a gift or a burden - who knows. but in this case, a 2-yr old FE-SEM is - usually a wonderful gift. The original gun was swapped out at just under 24 months of age ??? strange. if it is an FE-gun, it can live 'for ever', if not heavily used. - In our FE-SEM (from 1999), we had one for far more than 5 years -- with little / occasional use (before I got it). What a shame for such an instrument. The gun was fine. But, after 200 hrs, an FE-gun is not "well over the suggested run time" -- NO, NEVER. A tungsten filament may be ... but even then: if it still gives nice images, it was treated well, and it can stay there. You write: " it was still working & seemed stable" - then, why changing? (Although, exchanging a W filament is not too tricky, and from the budget, not too heavy). Basically, for all types of filaments, it is the usage AND the vacuum , which limits the lifetime. The level of vacuum which is required, is very different, for the different guns (W, LaB6, FE). IF you really have an FE-SEM, then you may ask the manufacturer for an appropriate treatment during extended periods of "no use". And they also should have a procedure for a proper re-start . They should ... Our FE-SEM and also our FE-TEM, both have one (and yes, for extended periods, I use the shut-down / re-start of the manufacturer). Works fine. - In our W-SEM and W-TEM, filaments are used until burnt. kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 Elected member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz
I agree with Reinhard. The specifics depend upon the type of FEG that you have. In general FEG microscopes require a high vacuum. The SEM and FIB in my lab had Schottky Field Emission guns. Like all FEGs, they required very high vacuums to work. The tip was always on. If your microscope is like this, you may use the microscope for only a few hours per month, but the tip is running all the time...
A complete shutdown of our FEG SEM and FIB for more than a day or so required a bake-out procedure to bring it back up. This could take a day or two to get the microscope back to working order. Like Reinhard's microscope, our microscopes had a "standby" mode where the gun was maintained under vacuum with the ion getters on and could be restarted easily and the tip turned back on in a few hours. I never tried using this mode for longer than a week or two. We typically needed to replace the tip every couple of years, but our microscopes were used daily. I recommend you contact the manufacturer and ask for their best practices for your microscope and use case.
Best regards, John Minter Retired from Kodak Analytical Sciences
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Email: jvazquez-at-fredhutch.org Name: Julio Vazquez
Organization: Fred Hutch Cancer Center
Title-Subject: [Filtered] job opportunity - Director Cellular Imaging at Fred Hutch
Message: Dear Microscopists,
Fred Hutch Cancer Center in Seattle has an opening for the position of Director of Cellular Imaging. The Cellular Imaging Core provides an extensive array of light and electron microscopy instrumentation and services to support research by Fred Hutch, University of Washington, and Seattle Children's Cancer Consortium researchers, as well as other local institutions and biotechs. The Core is currently expanding its capabilities in super-resolution and planning to bring in new capabilities including light sheet microscopy and Cryo-EM. Fed Hutch is located in the Seattle South Lake Union neighborhood, in the heart of a vibrant research and biotech community.
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X-from: Nessler, Randy A {randy-nessler-at-uiowa.edu}
Hi Naomi, I have had Bill service our HPF before. His contact info is:
Bill Graham BIBST LABS 37 Averill Road BROOKLINE, NH 03033
603-345-3887 BILL-at-BIBST.COM
Regards, Randy
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday, May 17, 2018 2:03 PM To: Nessler, Randy A
-------- Forwarded Message --------
X-from: naomi.kamasawa-at-mpfi.org
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Job Posting Title: Postdoctoral Research Associate Position on In-situ (Scanning) Transmission Electron Microscopy
The University of Connecticut (UConn), one of the top 20 public universities in the nation, invites applications for a Postdoctoral Research Associate position in the Department of Materials Science and Engineering (MSE) in the School of Engineering and the Institute of Materials Science (IMS). Applicants with a strong background in aberration-corrected (scanning) transmission electron microscopy ((S)TEM) and associated analytical techniques are encouraged to apply. In this position, you will have the opportunity to engage creative research on investigating structure-property correlations in advanced functional and structural materials including but not limited to heterogeneous catalysts. You will have the opportunity to work in a state-of-the-art TEM center hosting a probe-corrected Titan Themis STEM, and perform microscopy in the area of in-situ (gaseous) environmental (S)TEM, including new capability development. UConn is entering a transformational period of growth supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/) and the $1B Bioscience Connecticut (http://biosciencect.uchc.edu/) investments and a bold new Academic Plan: Path to Excellence (http://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1). As part of these initiatives, the new UConn-Thermo Fisher Scientific Center for Advanced Microscopy and Materials Analysis (CAMMA) has acquired seven new electron beam instruments including state-of-the-art TEM, SEM and FIB systems. These are housed in the UConn Technology Park as part of the purpose-built Advanced Characterization Laboratory, which opened earlier this year.
DUTIES AND RESPONSIBILITIES The successful candidate will share a deep commitment to transmission electron microscopy. Working under the supervision of Dr. Yuanyuan Zhu, the candidate will be expected to lead microscopy researches to further the understanding of materials dynamics. The candidate will also contribute to TEM sample preparation, mentor graduate and undergraduate students; write research proposals and progress reports; interact with research collaborators; prepare and maintain lab equipment and supplies, submit and publish peer reviewed journal papers.
MINIMUM QUALIFICATIONS An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A strong background and extensive research experience in TEM sample preparation (e.g. FIB liftout) and (scanning) transmission electron microscopy characterization. Good written and verbal communication skills. Good research capabilities as evidenced by a record of publication of results in peer-reviewed journals and external presentations at scientific conferences.
PREFERRED QUALIFICATIONS Additionally, the candidate is expected to be proficient at three or more of the following techniques including but not limited to: TEM Tilt-Series, SAED, Nanobeam Electron Diffraction, image-corrected HRTEM, probe-corrected STEM, EDS mapping, core- (and low-) loss EELS, in-situ heating TEM. Skills and experience in in-situ gas-cell microscopy is highly desired. Strong interpersonal skills including the ability to interact effectively with staffs, students and collaborators.
APPOINTMENT TERMS The selected candidate is expected to start in January 2019, or earlier if reach a mutual agreement. This is a full-time (12-month appointment) position, and is renewable every year. The successful candidates primary academic appointment will be at the UConn main campus in Storrs, CT. Salary will commensurate with qualifications and experience.
TO APPLY Please submit the following: acover letter;curriculum vitae (with a full list of publication), copies of two representative publicationstomailto:yuanyuan.2.zhu-at-uconn.edu , with a subject title In-situTEMPostdoc_yourname. Evaluation of applicants will begin immediately and continue until the position is filled. Employment of the successful candidate will be contingent upon the successful completion of a pre-employment criminal background check. All employees are subject to adherence to the State Code of Ethics, which may be found athttp://www.ct.gov/ethics/site/default.asp. ____________________________________________________________________ The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty, and staff. The diversity of students, faculty, and staff continues to increase, as does the number of honors students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and institutes serve the Universitys teaching, research, diversity, and outreach missions, leading to UConns ranking as one of the nations top research universities. UConns faculty and staff are the critical link to fostering and expanding our vibrant, multicultural, and diverse community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities, and members of traditionally underrepresented populations.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both chrisbrantner-at-gwu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
Organization: The George Washington University
Title-Subject: [Filtered] tannic acid-looking for a reference
Message: Good morning,
I am looking for a reference about using tannic acid as a tracer for damaged plasma membranes. Can anyone help me with this reference from an old journal? Or a different, more easily obtainable reference for the procedure?
Stain Technol. 1980 Nov;55(6):361-5. Tannic acid as an electron microscope tracer for permeable cell membranes.
Nnez-Durn H.
Thank you Chris Brantner George Washington University Nanofabrication and Imaging Center
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X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
It looks like a very basic instrument - but maybe that is what you are looking for. The price is less than what other vendors offer. The company also looks to be fairly new. That could mean that they are more aggressive in their pricing. I hope their engineering is good and that they will stand behind their work. They list options for multiple metals. That is good, because gold is not good for high magnification work (e.g., 50kx); you will see the structure of the deposited gold film. (We purchased an iridium coater when we got our FE-SEM.) I don't know what voltages is needed for the different metals. It seems they are limited to no more than 1600V. I can't tell if it is adjustable. Warren Straszheim, manager Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, October 31, 2018 7:30 AM To: Straszheim, Warren E [BIOTC]
-------- Forwarded Message --------
X-from: pscallio-at-dal.ca
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both khahn-at-med.unc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: khahn-at-med.unc.edu Name: Klaus Hahn
Organization: UNC - Chapel Hill
Title-Subject: [Filtered] Position for microscopist / optical engineer
Message: Staff scientist at UNC-Chapel Hill: Microscopist / Optics engineer
We are looking for a person with a strong background in microscopy and/or optical engineering to develop new microscopes for live cell imaging. The Hahn lab designs proteins and small molecules to visualize and control signaling (see hahnlab.com) and is now working closely with engineering and computational biology groups to engineer new microscopes and harness capabilities of novel proteins and dyes (eg Legant, Superfine, Elston groups at UNC). You will assemble and focus on microscopes with diverse capabilities, including extensions of single particle tracking, PALM/STORM, SIM-TIRF, lattice light sheet and spin TIRF. In addition to optics, experience in tissue culture, biosensors/optogenetics, or cell biology would be a plus. The candidate should be familiar with at least one of the following programming languages: MATLAB, LabVIEW, Python, Java, R, etc. Experience with Metamorph and Micromanager/ImageJ. A PhD is preferred and a minimum of a bachelors degree with relevant experience is required. Please send your CV and cover letter s to Klaus Hahn, khahn-at-med.unc.edu.
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my colleague Tyler emailed the list about a year ago asking for peoples' experience with the JEOL 2100F high-tilt (HT) pole piece. We got one really helpful response from someone with a similar instrument. We now have a more basic question: do you have a JEOL 2100F with a high-tilt pole piece and do you mind if we bother you with a few quick questions about the performance of your microscope? (If you have a 2010F with a high-tilt pole piece, we'd also be interested to talk to you.) It seems that this is a relatively rare pole piece.
Thanks, Thomas
-- Thomas Danz IV. Physikalisches Institut Georg-August-Universität Göttingen Friedrich-Hund-Platz 1 D-37077 Göttingen
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Email: nikd-at-vsl.cua.edu Name: Nik Deems
Organization: Vitreous State Laboratory
Title-Subject: [Filtered] Features function AZtec 3.3 and up
Message: Hi all,
My colleagues and I just upgraded our EDS and our software to AZtec 3.3. We are trying to make a go at the Features function and are having some difficulty doing what we need to do (basically find the percentage of the whole of the image of a set of features based on contrast threshold).
If anyone if familiar with this function, could you please e-mail me?
Cheers, Nik Deems
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Title-Subject: [Filtered] automatic contrasting system for ultrathin sections
Message: Hello all,
our 25 years old automatic contrasting system is not working any more. Do you have a recommendation / good experience with a new instrument? I am thinking about the Leica AC20. But I was wondering if there are other companies than Leica which sell an instrument for contrasting and provide service in Germany? Thank you very much for your help! Best wishes, Agnes Matysiak Login Host: 88.152.59.92 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
We are Core Facility doing mostly biology TEM. Our 2 Tecnai 12s are getting old and at some point I amafraid we will not be able to find computer boards for failed obsolete old ones. So, we are thinking to replace at least one of them with some modern alternative. As we are mostly doing bright field standard TEM with plasticembedded samples or negatively stained biology materials then the choice goes to 3 options: FEI Talos 120, Jeol 1400Flash or Hitachi 7800 series. We rarely do STEM but it could be of benefit to have this option. I would like to have some feedback on these instrumentsregarding value for money, how robust they are,how good and fast service is in your local area, how good user interface is, how steep is a learning curvefor an inexperienced user and will it be worthwhile to learn new interface if we will move away from FEI.
We need just workhorse instrument that will not break often and will be simple to use. Also I am interested in your opinion of how useful some particular features for specific instruments:
1) Hitachi HT7800 series:
- How good Dual-Mode objective lens is? Is it worthwhile?
2) Jeol 1400Flash:
- How good is OM image linkage function? What it is doing exactly?
3) I have a feeling that FEI Talos is a bit overpriced in comparison to its rivals. Is it correct?
I would really appreciate any insights about real life issues and experiences with these instruments.
Please, reply to my UK e-mail address to avoid any collisions on the list.
Sincerely,
Alex
-- Dr. Aleksandr Mironov MD, PhD Senior Experimental Officer D.1527, M.Smith Building EM Core Facility School of Biological Sciences, Faculty of Biology Medicine and Health University of Manchester Oxford Road Manchester M13 9PT UK
Research Scientist Materials Research Laboratory University of Illinois at Urbana-Champaign
The Materials Research Laboratory (MRL) at the University of Illinois at Urbana-Champaign is seeking a highly motivated Research Scientist to work in its Electron Microscopy instrumentation core facility. The Research Scientist will serve as a member of the MRL Central Research Facilities staff with a focus on enabling soft materials and biological imaging research by training and advising users in electron microscopy and related methods (including specimen preparation), and by engaging in collaboration with various local and regional users on active research projects. The individual's job responsibilities include:
* Supporting research on soft materials and biological imaging in MRL by serving as the technical point of contact to the research community. Activities include managing user interactions, developing research proposals, and assisting with data interpretation. * Developing expertise in the instruments and techniques by remaining abreast of advances in the field to advance the local research community's proficiency in the techniques. Activities in this area include ongoing review of relevant scientific literature, participation in professional societies and conference/workshop/training opportunities, and advising facilities leadership on emerging instrumentation needs. * Training and advising users in electron microscopy and related methods, including preparation of soft materials and biological samples for electron imaging. Activities beyond hands-on training include developing and improving training materials as needed (based on developments in the field, maintenance or service alerts, and user feedback), and engaging in collaborative research projects with various local and regional users by providing direct support in electron imaging and assistance in data interpretation. * Coordinating instrument updates and/or major repairs with manufacturers/vendors, other support personnel and facilities leadership as appropriate. Specific activities include: assuring that instrumentation is kept in optimum working condition by actively monitoring its usage and performance against established metrics; developing standard schedules for routine maintenance based upon manufacturer documentation, use patterns and facilities best practices; performing routine maintenance in conjunction with other supporting staff; and troubleshooting the root cause of suboptimal performance and making minor repairs as needed. * Assisting with the MRL's outreach activities, including participating in the development of new research initiatives and providing improvements to existing outreach programs. * Performing additional research scientist duties to promote the unit's mission as needed.
The successful candidate should demonstrate excellent verbal and written communication skills and must be able to work independently and proactively with multiple users. The candidate must have a Bachelor's degree or higher in physical or life sciences or engineering. The candidate must have one to three years of demonstrated, hands-on experience with electron microscopy. Preference will be given to candidates with a Master's degree, Ph. D, or a Bachelor's degree and three or more years of demonstrated, hands-on experience with biological or soft materials electron microscopy, including handling of biohazardous materials. Experience with cryo-electron microscopy instrumentation and techniques, including cryo-ultramicrotomy and cryo-plunge, and image analysis is preferred.
This is a full-time, benefits-eligible academic professional position appointed on a 12-month service basis. The expected start date is as soon as possible. Salary is commensurate with experience and qualifications. To apply, please complete a candidate profile at http://jobs.illinois.edu {http://jobs.illinois.edu/} and upload the following as a single file: a cover letter, resume, and the names and contact information for three professional references. To ensure full consideration, all requested information must be submitted by December 15, 2018. Applicants may be interviewed before the closing date; however, no hiring decision will be made until after that date.
The successful candidate will become a member of the dedicated staff of about 20 scientists and engineers, who maintain major research instruments in the MRL's core facilities for soft materials characterization, scanning probe microscopy, laser and optical spectroscopy, electron microscopy, surface analysis, x-ray diffraction, and nanofabrication facilities including two cleanrooms. Over 1,000 researchers from across our campus as well as other academic institutions, industry, and national laboratories use the facilities, logging more than 100,000 user hours annually. The lab is recognized as one of the premier mid-sized user facilities in the nation. For details, please visit us online at https://mrl.illinois.edu/facilities/.
The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer.
The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women, veterans and individuals with disabilities are encouraged to apply. For more information, visit http://go.illinois.edu/EEO. To learn more about the University's commitment to diversity, please visit http://www.inclusiveillinois.illinois.edu
Thanks,
Mauro ___________________________________ Mauro Sardela, Ph.D. Director, Central Research Facilities Materials Research Laboratory University of Illinois at Urbana-Champaign 104 S. Goodwin Avenue - Urbana IL 61801 (217) 244 0547 | office # SC2014 www.mrl.illinois.edu {http://www.mrl.illinois.edu/
_______________________________________________
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Hi Everyone, I have a user cutting thin sections of a polymer that is soluble in water, methanol, ethanol and other organic solvents.
Any thoughts about alternatives liquids?
Chris
Christopher J. Gilpin Ph.D. Campus-wide Coordinator for Electron Microscopy Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx skype cjgilpin
==============================Original Headers============================== 7, 32 -- From gilpin-at-purdue.edu Wed Nov 28 12:14:34 2018 7, 32 -- Received: from xppmailspam03.itap.purdue.edu (xppmailspam03.itap.purdue.edu [128.210.5.14]) 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wASIEXSB027707 7, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2018 12:14:34 -0600 7, 32 -- X-IronPort-Anti-Spam-Filtered: true 7, 32 -- X-IronPort-AV: E=Sophos;i="5.56,291,1539662400"; 7, 32 -- d="scan'208";a="187968638" 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) 7, 32 -- by xppmailspam03.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 28 Nov 2018 13:18:39 -0500 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc06.purdue.lcl 7, 32 -- (172.30.136.179) with Microsoft SMTP Server (TLS) id 15.0.1365.1; Wed, 28 Nov 7, 32 -- 2018 13:18:39 -0500 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 7, 32 -- 15.00.1365.000; Wed, 28 Nov 2018 13:18:39 -0500 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 32 -- Subject: picking up sections from something other than water etc 7, 32 -- Thread-Topic: picking up sections from something other than water etc 7, 32 -- Thread-Index: AdSHRpr7NYB65GQiSL+lCHZqyib3Zg== 7, 32 -- Date: Wed, 28 Nov 2018 18:18:39 +0000 7, 32 -- Message-ID: {3ffc8c253b6f496bb8e9650dc645b941-at-wppexc03.purdue.lcl} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 32 -- x-originating-ip: [10.163.23.200] 7, 32 -- Content-Type: text/plain; charset="utf-8" 7, 32 -- MIME-Version: 1.0 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id wASIEXSB027707 ==============================End of - Headers==============================
How about fully fluorinated liquids, such as Galden by Inland Vacuum? Another one of chemically inert variants, such as Perfluorodecalin maybe?
Valery Ray (also with REFINE/UCONN) =================================== E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 MEO Engineering / PBS&T 290 Broadway, Suite 298 Methuen, MA 01844, USA www.partbeamsystech.com www.freudlabs.com
On 11/28/2018 1:14 PM, gilpin-at-purdue.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Everyone, } I have a user cutting thin sections of a polymer that is soluble in water, methanol, ethanol and other organic solvents. } } Any thoughts about alternatives liquids? } } ​​​​​ } Chris } } Christopher J. Gilpin Ph.D. } Campus-wide Coordinator for Electron Microscopy } Director, Life Science Microscopy Facility } Purdue University } Whistler Hall of Agriculture Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 } gilpin-at-purdue.edu } lsmf-at-purdue.edu reaches everyone in the facility. } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } skype cjgilpin } } } } } ==============================Original Headers============================== } 7, 32 -- From gilpin-at-purdue.edu Wed Nov 28 12:14:34 2018 } 7, 32 -- Received: from xppmailspam03.itap.purdue.edu (xppmailspam03.itap.purdue.edu [128.210.5.14]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wASIEXSB027707 } 7, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2018 12:14:34 -0600 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true } 7, 32 -- X-IronPort-AV: E=Sophos;i="5.56,291,1539662400"; } 7, 32 -- d="scan'208";a="187968638" } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } 7, 32 -- by xppmailspam03.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 28 Nov 2018 13:18:39 -0500 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by wppexc06.purdue.lcl } 7, 32 -- (172.30.136.179) with Microsoft SMTP Server (TLS) id 15.0.1365.1; Wed, 28 Nov } 7, 32 -- 2018 13:18:39 -0500 } 7, 32 -- Received: from wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174]) by } 7, 32 -- wppexc03.purdue.lcl ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id } 7, 32 -- 15.00.1365.000; Wed, 28 Nov 2018 13:18:39 -0500 } 7, 32 -- From: "Gilpin, Christopher J" {gilpin-at-purdue.edu} } 7, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 7, 32 -- Subject: picking up sections from something other than water etc } 7, 32 -- Thread-Topic: picking up sections from something other than water etc } 7, 32 -- Thread-Index: AdSHRpr7NYB65GQiSL+lCHZqyib3Zg== } 7, 32 -- Date: Wed, 28 Nov 2018 18:18:39 +0000 } 7, 32 -- Message-ID: {3ffc8c253b6f496bb8e9650dc645b941-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted } 7, 32 -- x-originating-ip: [10.163.23.200] } 7, 32 -- Content-Type: text/plain; charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id wASIEXSB027707 } ==============================End of - Headers============================== }
Not so sure these would be compatible with Diamond knives and their mounting cements.
Best to ask Helmut Gnaegi of Diatome for ideas how to do this Chris helmut.gnaegi-at-diatome.ch
Al Coritz Electron Microscopy Sciences & Diatome US
-----Original Message----- X-from: vray-at-partbeamsystech.com {vray-at-partbeamsystech.com} Sent: Wednesday, November 28, 2018 1:59 PM To: Al Coritz {acoritz-at-emsdiasum.com}
How about fully fluorinated liquids, such as Galden by Inland Vacuum? Another one of chemically inert variants, such as Perfluorodecalin maybe?
Valery Ray (also with REFINE/UCONN) =================================== E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 MEO Engineering / PBS&T 290 Broadway, Suite 298 Methuen, MA 01844, USA www.partbeamsystech.com www.freudlabs.com
On 11/28/2018 1:14 PM, gilpin-at-purdue.edu wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } Hi Everyone, } I have a user cutting thin sections of a polymer that is soluble in water, methanol, ethanol and other organic solvents. } } Any thoughts about alternatives liquids? } } ​​​​​ } Chris } } Christopher J. Gilpin Ph.D. } Campus-wide Coordinator for Electron Microscopy Director, Life Science } Microscopy Facility Purdue University Whistler Hall of Agriculture } Research, Room S052 } 170 S. University St } West Lafayette, IN 47907 } 765-494-7750 } gilpin-at-purdue.edu } lsmf-at-purdue.edu reaches everyone in the facility. } http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx } skype cjgilpin } } } } } ==============================Original } Headers============================== } 7, 32 -- From gilpin-at-purdue.edu Wed Nov 28 12:14:34 2018 7, 32 -- } Received: from xppmailspam03.itap.purdue.edu (xppmailspam03.itap.purdue.edu [128.210.5.14]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wASIEXSB027707 } 7, 32 -- for {microscopy-at-microscopy.com} ; Wed, 28 Nov 2018 12:14:34 -0600 } 7, 32 -- X-IronPort-Anti-Spam-Filtered: true 7, 32 -- X-IronPort-AV: } E=Sophos;i="5.56,291,1539662400"; } 7, 32 -- d="scan'208";a="187968638" } 7, 32 -- Received: from exchange.purdue.edu ([128.210.1.29]) } 7, 32 -- by xppmailspam03.itap.purdue.edu with ESMTP/TLS/AES256-SHA; 28 Nov 2018 13:18:39 -0500 } 7, 32 -- Received: from wppexc03.purdue.lcl (172.30.136.176) by } wppexc06.purdue.lcl 7, 32 -- (172.30.136.179) with Microsoft SMTP } Server (TLS) id 15.0.1365.1; Wed, 28 Nov 7, 32 -- 2018 13:18:39 -0500 } 7, 32 -- Received: from wppexc03.purdue.lcl } ([fe80::3c86:dcb8:fd99:f174]) by 7, 32 -- wppexc03.purdue.lcl } ([fe80::3c86:dcb8:fd99:f174%15]) with mapi id 7, 32 -- } 15.00.1365.000; Wed, 28 Nov 2018 13:18:39 -0500 7, 32 -- From: } "Gilpin, Christopher J" {gilpin-at-purdue.edu} 7, 32 -- To: } "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 32 -- } Subject: picking up sections from something other than water etc 7, 32 } -- Thread-Topic: picking up sections from something other than water } etc 7, 32 -- Thread-Index: AdSHRpr7NYB65GQiSL+lCHZqyib3Zg== 7, 32 -- } Date: Wed, 28 Nov 2018 18:18:39 +0000 7, 32 -- Message-ID: } {3ffc8c253b6f496bb8e9650dc645b941-at-wppexc03.purdue.lcl} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 32 -- } x-originating-ip: [10.163.23.200] 7, 32 -- Content-Type: text/plain; } charset="utf-8" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- } X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id } wASIEXSB027707 ==============================End of - } Headers============================== }
The following position is open until Tuesday, 18 December.
Experimental Scientist - Black Mountain MicroImaging Centre (BMIC)
If interested, please find further information and apply ONLINE: https://jobs.csiro.au/job/Canberra%2C-ACT-Experimental-Scientist-Black-Mountain-MicroImaging-Centre-%28BMIC%29/520162300/?locale=en_GB Please do not reply to this email.
The Black Mountain MicroImaging Centre (BMIC) is a state-of-the-art Centre which builds and fosters human and scientific capabilities, to produce innovative cross-discipline research and deliver impact in crop science. BMIC is equipped with general PC2 lab facilities, confocal, fluorescent, scanning electron, dissecting and brightfield microscopes, embedding and sectioning equipment and Fourier Transform Infra-Red spectroscope/microscope.
Your duties will include Under the general direction of the BMIC manager, participate in planning of the microscopy component of a wide range of projects focussed on crops and other plants, with responsibility for the scheduling and completion of project work, including allocating and directing tasks where appropriate and documentation of methods and procedures. Provide timely, accurate and professional advice to staff about structural and developmental aspects of their research. Adapt and/or develop original experimental methods, concepts and ideas for the imaging component of existing and further research, promptly addressing where methods may not be defined. Initiative is required in seeking new approaches to meet experimental objectives. Develop high-level expertise in various types of microscopy, and/or digital image analysis and image management. Make significant contributions to the interpretation and communication, both internal and external, of research results and collaboration on drafting and delivering presentations to, and/or detailed written reports for, clients and the scientific and technology community. A significant component of this position is to provide on-the-job training and instruction in microscopy, imaging, and other relevant instruments and techniques to colleagues, including visitors, students and postdocs, and managing resources to meet objectives, as required. Ensure the smooth operation of BMIC by carrying out the role of lab custodian. This includes maintaining adequate levels of consumables, keeping the laboratories tidy, assisting in upkeep of specialised equipment and software, conducting regular PC2 audits and promoting best HSE practices in BMIC.
Salary: $AUD 82,450 to $SAUD 93,280 plus up to 15.4% Superannuation Tenure: Indefinite
Essential Criteria: Demonstrated knowledge of, and experience in analysis of plant structure and functional plant anatomy. Demonstrated experience in specimen preparation for light and electron microscopy and in use of light, fluorescence, confocal and electron microscopes. Experience in digital imaging and image analysis. Proven ability to work with minimum supervision, both independently and as part of a team, on a variety of research topics. Demonstrated ability to use initiative in solving technical and research problems. Demonstrated ability to organise data and resources, and to work to defined timelines and milestones. Excellent written, oral and interpersonal communication skills, including the ability to liaise with staff at all levels with professionalism. This includes the ability to train staff and students on the use of specialized equipment as well as provide basic guidance in the design of experiments using these instruments.
Desirable Criteria: Experience in presentation of results at conferences and in publication of scientific papers. Experience in cross-program and cross-institution research collaborations. Demonstrated knowledge and experience in the specialist areas of image analysis, including deconvolution and 3D reconstruction will be considered highly desirable. Experience in resin-embedding, sectioning and immunostaining of plant tissues for light and electron microscopy. Experience in lab management.
cheers, Rosemary
Dr Rosemary White CSIRO Black Mountain GPO Box 1700 Canberra, ACT 2601 Australia
T 61 2 6246 5475 M 61 468 966 713
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sorry for replying late. I would simply do histology with serial sectionning. staining collagen in histology is standard, most histology labs should be able to do it. You probably don't need the whole piece of tissue to determine the orientation of the fibers.
Best regards, Stephane On Sunday, October 28, 2018, 1:26:36 AM GMT+2, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
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Title-Subject: [Filtered] Imaging of pig pericardium
Message: We are looking for a suitable technique to establish the 3D microstructure of a decellularized sheet of swine pericardium (~15cmx8cmx0.5cm), with high enough resolution to visualize the orientation and 3-D arrangement of collagen bundles and detect anomalies or inflection points. The large size of the specimen makes it impractical for uCT. We were thinking of confocal stereomicroscopy using collagen autofluorescence. We welcome practical suggestions. Thank you!
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My Philips CM12 TEM has a tendency to shut off if I lift the focus screen too fast (too hard?) to expose the bottom mount camera. This is relatively new to me, as I was using a side mount camera and never had to lift the plate.
Is this normal?
If it's not, other than being slow and delicate, what was the cure?
Oh, yes, if you don't celebrate Christmas, that fine. I wish you the very best at this time of year.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
} } } 05.12.2018 at 17:06: } My Philips CM12 TEM has a tendency to shut off if I lift the focus screen too } fast (too hard?) to expose the bottom mount camera. This is relatively new } to me, as I was using a side mount camera and never had to lift the plate.
Dear Frank, have a CM12 since 1990 ( oh yes, I remember it VERY WELL - the boxes were delivered on Dec 4th, 1990, two days before St. Nicklas day ...) and it is still in use. I had quite a few pecularities, but never heard of this one. No, this is NOT normal ... for sure. We have a bottom-mount camera in operation (TVIPS) since 1998, daily, heavy used - no problems with any of the phosphor screens ... and I keep my fingers crossed that we will never have a problem like this. I only hope that any of the many users out there can help ... kind regards, have a nice December, merry Xmas and happy new year! Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 Elected member of the IFSM board
Next microscopy conferences: - Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin - EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference) - MC2021 in Vienna (D-A-CH conference) - next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz
==============================Original Headers============================== 7, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Wed Dec 5 10:51:28 2018 7, 25 -- Received: from rrzmta1.uni-regensburg.de (rrzmta1.uni-regensburg.de [194.94.155.51]) 7, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wB5GpRbU019885 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Dec 2018 10:51:28 -0600 7, 25 -- Received: from rrzmta1.uni-regensburg.de (localhost [127.0.0.1]) 7, 25 -- by localhost (Postfix) with SMTP id E3CA611323 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Dec 2018 17:55:55 +0100 (CET) 7, 25 -- Received: from gwsmtp1.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 7, 25 -- by rrzmta1.uni-regensburg.de (Postfix) with ESMTP id C68D85E557 7, 25 -- for {Microscopy-at-microscopy.com} ; Wed, 5 Dec 2018 17:55:55 +0100 (CET) 7, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp1.uni-regensburg.de 7, 25 -- with Novell_GroupWise; Wed, 05 Dec 2018 17:55:55 +0100 7, 25 -- Message-Id: {5C08031A020000540007C7EF-at-gwsmtp1.uni-regensburg.de} 7, 25 -- X-Mailer: Novell GroupWise Internet Agent 18.1.0 7, 25 -- Date: Wed, 05 Dec 2018 17:55:54 +0100 7, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 7, 25 -- To: {frank_karl-at-ardl.com} , "microscopy server" {Microscopy-at-microscopy.com} 7, 25 -- Subject: Screen lifter blues 7, 25 -- References: {201812051606.wB5G614m010169-at-microscopy.com} 7, 25 -- In-Reply-To: {201812051606.wB5G614m010169-at-microscopy.com} 7, 25 -- Mime-Version: 1.0 7, 25 -- Content-Type: text/plain; charset=US-ASCII 7, 25 -- Content-Disposition: inline 7, 25 -- Content-Transfer-Encoding: 8bit 7, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id wB5GpRbU019885 ==============================End of - Headers==============================
Hello Frank, It is really strange as Reinhard wrote. We have been running CM12 since 1985 up to 2014 when HT tank has gone. We had some problems with leaks in viewing chamber (o-ring of diffraction beam stopper assembly and sealing of 35 mm camera) during those years. But those leaks never led to the microscope complete switch-off. Usually HT was switched off and IGP pump too. However, we observed that after strong discharge the CM12 was sometimes switched off and this also restarted the EDAX system connected to the CM12.
What tells you the plate exposure meter when you change the intensity on the main screen? Is the exposure time changing?
I hope that you solve the problem soon.
Best regards and Merry Christmas
Oldrich
-- Oldrich Benada Institute of Microbiology, Czech Acad. Sci. Videnska 1083 142 20 Prague 4 Czech Republic
On Wed, 5 Dec 2018 10:05:28 -0600 frank_karl-at-ardl.com wrote:
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==============================Original Headers============================== 13, 45 -- From benada-at-biomed.cas.cz Wed Dec 5 14:14:05 2018 13, 45 -- Received: from barracuda.biomed.cas.cz (barracuda.biomed.cas.cz [147.231.40.11]) 13, 45 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wB5KE4eE002534 13, 45 -- for {microscopy-at-microscopy.com} ; Wed, 5 Dec 2018 14:14:05 -0600 13, 45 -- X-ASG-Debug-ID: 1544041111-05011e63e66154b0001-4CH8be 13, 45 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) by barracuda.biomed.cas.cz with ESMTP id AcwI0FqFUbhl45GK (version=TLSv1.2 cipher=ECDHE-RSA-AES256-GCM-SHA384 bits=256 verify=NO); Wed, 05 Dec 2018 21:18:31 +0100 (CET) 13, 45 -- X-Barracuda-Envelope-From: benada-at-biomed.cas.cz 13, 45 -- X-Barracuda-Effective-Source-IP: mail2.biomed.cas.cz[147.231.40.32] 13, 45 -- X-Barracuda-Apparent-Source-IP: 147.231.40.32 13, 45 -- Received: from localhost (unknown [185.186.248.27]) 13, 45 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 13, 45 -- (No client certificate requested) 13, 45 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id A4AA2D0187A; 13, 45 -- Wed, 5 Dec 2018 21:18:29 +0100 (CET) 13, 45 -- Date: Wed, 5 Dec 2018 21:18:32 +0100 13, 45 -- From: =?UTF-8?Q?Old=C5=99ich?= Benada {benada-at-biomed.cas.cz} 13, 45 -- To: frank_karl-at-ardl.com, {microscopy-at-microscopy.com} 13, 45 -- Subject: Re: [SPAM] [Microscopy] Screen lifter blues 13, 45 -- Message-ID: {20181205211832.00002ac3-at-biomed.cas.cz} 13, 45 -- X-ASG-Orig-Subj: Re: [SPAM] [Microscopy] Screen lifter blues 13, 45 -- In-Reply-To: {201812051605.wB5G5SBe009652-at-microscopy.com} 13, 45 -- References: {201812051605.wB5G5SBe009652-at-microscopy.com} 13, 45 -- X-Mailer: Claws Mail 3.17.1 (GTK+ 2.24.32; x86_64-w64-mingw32) 13, 45 -- MIME-Version: 1.0 13, 45 -- Content-Type: text/plain; charset=US-ASCII 13, 45 -- Content-Transfer-Encoding: 7bit 13, 45 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 13, 45 -- X-IoP-CAS-MailScanner-ID: A4AA2D0187A.A895E 13, 45 -- X-IoP-CAS-MailScanner: Processed 13, 45 -- X-Spam-Status: No 13, 45 -- X-Barracuda-Connect: mail2.biomed.cas.cz[147.231.40.32] 13, 45 -- X-Barracuda-Start-Time: 1544041111 13, 45 -- X-Barracuda-Encrypted: ECDHE-RSA-AES256-GCM-SHA384 13, 45 -- X-Barracuda-URL: https://barracuda.biomed.cas.cz:443/cgi-mod/mark.cgi 13, 45 -- X-Virus-Scanned: by bsmtpd at biomed.cas.cz 13, 45 -- X-Barracuda-Scan-Msg-Size: 9516 13, 45 -- X-Barracuda-BRTS-Status: 1 13, 45 -- X-Barracuda-Spam-Score: 1.00 13, 45 -- X-Barracuda-Spam-Status: No, SCORE=1.00 using per-user scores of TAG_LEVEL=1000.0 QUARANTINE_LEVEL=7.0 KILL_LEVEL=1000.0 tests=BSF_RULE7568M, WEIRD_PORT 13, 45 -- X-Barracuda-Spam-Report: Code version 3.2, rules version 3.2.3.63130 13, 45 -- Rule breakdown below 13, 45 -- pts rule name description 13, 45 -- ---- ---------------------- -------------------------------------------------- 13, 45 -- 0.50 WEIRD_PORT URI: Uses non-standard port number for HTTP 13, 45 -- 0.50 BSF_RULE7568M Custom Rule 7568M ==============================End of - Headers==============================
It is a little unclear what you mean by the Microscope "shuts off". Is it just the HT, the vacuum system, or all the electronics?
Assuming that it isn't all the electronics, it sounds like there is a vacuum leak on the screen lift lever. If you are operating, the loss of vacuum will shut off the emitter and the HT and (quite likely) the IGPs as well. The vacuum page will then show the IGP shut off and V6 open so that the Diff pump is pumping on the column and gun.
The fix would be to disassemble the screen lift feedthrough and replace the O-rings.
Cheers, Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
------ Original Message ------ X-from: "frank_karl-at-ardl.com" {frank_karl-at-ardl.com} To: "Colijn, Hendrik" {colijn.1-at-osu.edu} Sent: 12/5/2018 11:09:55 AM
Hello Everyone, Merry Christmas.
My Philips CM12 TEM has a tendency to shut off if I lift the focus screen too fast (too hard?) to expose the bottom mount camera. This is relatively new to me, as I was using a side mount camera and never had to lift the plate.
Is this normal?
If it's not, other than being slow and delicate, what was the cure?
Oh, yes, if you don't celebrate Christmas, that fine. I wish you the very best at this time of year.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
The Scanning Probe Microscopy group at ORNL is looking for technical professional in the field of ultra high vacuum SPM. The description of the position is available at: https://jobs.ornl.gov/job/Oak-Ridge-Technical-Associate-Staff-Member-UHV-Scanning-Probe-Microscopy-TN-37830/521071900/
Sergei
-- Sergei V. Kalinin
Director, Institute for Functional Imaging of Materials
Fellow MRS, IEEE, APS, IoP, AVS
Oak Ridge National Laboratory
Phone: (865) 241-0236
==============================Original Headers============================== 8, 37 -- From sergei2-at-ornl.gov Thu Dec 6 12:02:10 2018 8, 37 -- Received: from mta02.ornl.gov (mta02.ornl.gov [128.219.177.136]) 8, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wB6I2An4024070 8, 37 -- for {microscopy-at-microscopy.com} ; Thu, 6 Dec 2018 12:02:10 -0600 8, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 8, 37 -- d=ornl.gov; i=-at-ornl.gov; q=dns/txt; s=p20151116; 8, 37 -- t=1544119603; x=1575655603; 8, 37 -- h=to:from:subject:message-id:date:mime-version: 8, 37 -- content-transfer-encoding; 8, 37 -- bh=K5kpZeVbGCPXn1DNj7f3uhvCogOj+HLkrGQRm8xHSPM=; 8, 37 -- b=gfis5ISivSiU8Ne49/iEdlY5E2U+Vp11PhQuWPPwmtufuk5nApGHqGTc 8, 37 -- sw65P3u+nFsnVDE0VJKRwn0dFoKzWr9JQknfsCDqTslkI65UZdyAzTRxb 8, 37 -- welrvTk2e4ACooEh9kNzubYai2qJvXR6KCYOq5f0X2rAeKT/1A11rqhPq 8, 37 -- rz+Xdie95ohPoFe5PxTDhYkYM4GwaziQSIZUXZmJilqWpSi3WvuhSFBeL 8, 37 -- 7dceR6VX9RcnqZQziY8zCYmsruTj7bwzoRJOlbqEd2wIiwLI3sTKXWn1g 8, 37 -- i1RT0dh2rIpycvIX8Fa6Fj/I3/0Mmd94x8nnx5R/iIXfSavm8nsDFj3bd 8, 37 -- w==; 8, 37 -- X-SG: RELAYLIST 8, 37 -- X-IronPort-AV: E=Sophos;i="5.56,323,1539662400"; 8, 37 -- d="scan'208";a="52831713" 8, 37 -- Received: from emgwy2.ornl.gov ([160.91.254.10]) 8, 37 -- by iron2.ornl.gov with ESMTP/TLS/DHE-RSA-AES256-GCM-SHA384; 06 Dec 2018 13:06:39 -0500 8, 37 -- Received: from [128.219.192.116] (pc95228.ornl.gov [128.219.192.116]) 8, 37 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 37 -- (No client certificate requested) 8, 37 -- by emgwy2.ornl.gov (Postfix) with ESMTPS id 439k7g3RnPz2Ss8B 8, 37 -- for {microscopy-at-microscopy.com} ; Thu, 6 Dec 2018 13:06:39 -0500 (EST) 8, 37 -- To: microscopy-at-microscopy.com 8, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov} 8, 37 -- Subject: Technical professional position open - SPM 8, 37 -- Message-ID: {5C09652F.6010601-at-ornl.gov} 8, 37 -- Date: Thu, 6 Dec 2018 13:06:39 -0500 8, 37 -- User-Agent: Mozilla/5.0 (Windows NT 6.1; WOW64; rv:38.0) Gecko/20100101 8, 37 -- Thunderbird/38.0.1 8, 37 -- MIME-Version: 1.0 8, 37 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 37 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
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Message: The Microanalysis Society is pleased to announce registration for QMA 2019, Quantitative Microanalysis 2019, is now open. QMA 2019 will be held at the University of Minnesota, Minneapolis, June 24-27, 2019. The conference will include user meetings, tutorials, technical platform and poster presentations, vendor demonstrations, group discussions, and a conference banquet. Topics covered include, but are not limited to, quantitative analysis by SEM/EDS and EPMA, compositional mapping, sample preparation, microanalysis education, and reference materials.
Financial support for early career scholars, including undergraduate, graduate, post-doctoral, and early career professional scientists who are within two years of their last degree is available through reimbursement. A letter describing the details of this award are available on the website. Visit the QMA 2019 website for updated information regarding registration, the technical program, ECS financial support, travel to the venue, and accommodations. https://the-mas.org/events/topical-conferences/qma-2019/
Please join us! The QMA 2019 Organizing Committee
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Message: The Department of Materials Science and Engineering at Carnegie Mellon University in Pittsburgh, PA, is looking for a Materials Characterization Facilities Specialist to work in the Materials Characterization Facility. Interested parties should visit the following web site for more details:
Prof Hellmuth Sitte passed away on Thursday 6th December in Innsbruck, aged 90.
Prof Sitte combined his professor and rectorship at the university of the Saarland in Homburg (Bio Medicine) with a deep interest and dedication for the development of instrumentation for electron microscopy. A pioneer in ultramicrotome design he had over 200 patents in the field of ultramicrotomy and cryo instrumentation to his name.
Many of you will have fond memories of him through the workshops that were conducted in Seefeld in Tirol where he introduced participants and guest staff not only to his passion for science and instrumentation, but also for his beloved mountains of the Northern Alps and the cultural wealth of the Innsbruck valley and South Tirol.
He and his family were amazing and welcoming hosts for many of us and the social evenings in his home were second to none.
These combined aspects have left a deep and unique impression.
The Porto AFM Workshop 2019 has been announced. This is the sixth edition of the course.
The course will run from the 15th to 18th April. This is a training workshop aimed at any researcher or scientist who wants to learn about Atomic Force Microscopy, or increase their knowledge of the technique. Following the successful courses that have run since 2011, the course will include several hours hands-on training in acquiring images with the AFM as well as AFM data processing. Lectures cover instruments, sample preparation, AFM operation, data processing and applications.
For more information visit http://afmhelp.com/course, or email me at afmhelp-at-gmail.com
Dr. Peter Eaton
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Hello Everyone, Merry Christmas and Happy New Year.
I've always said I'm not really a TEM guy, but a PLM generalist with a side dish of SEM/EDS. ARDL is looking for a local TEM consultant to show us how to maximize alignment including filament changing.
While we do this when we have to, we use to depend heavy on our FEI service guy. Unfortunately, FEI no longer provides service for our CM12 and while our current contractor, TSS, does a wonderful job, they can't stop by and help tweek the scope when I fumble finger it. Just because I'm semi-retired doesn't mean I don't want to learn more..................
If you're a local to the Akron area, we would be interested in a little onsite training and assistance. Please contact me at: Frank_Karl-at-ARDL.Com.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
==============================Original Headers============================== 13, 67 -- From frank_karl-at-ardl.com Wed Dec 12 09:45:36 2018 13, 67 -- Received: from NAM02-CY1-obe.outbound.protection.outlook.com (mail-eopbgr760081.outbound.protection.outlook.com [40.107.76.81]) 13, 67 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id wBCFjaHJ017070 13, 67 -- for {microscopy-at-microscopy.com} ; Wed, 12 Dec 2018 09:45:36 -0600 13, 67 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 13, 67 -- d=ardlcorp.onmicrosoft.com; s=selector1-ardl-com; 13, 67 -- h=From:Date:Subject:Message-ID:Content-Type:MIME-Version:X-MS-Exchange-SenderADCheck; 13, 67 -- bh=ipso5AoldL2pYdyrQr30O6KccWOTtBmjn5GxIt2mKGE=; 13, 67 -- b=ZxyOVyH5godXcx5QOlkRKKEiHwXBU+wM49L7GAtkSJVxVjQ1sQnTOqOafo3Z3lOOoY2n9VguOMa+u4hjgLthZrETLu98PpdQ9+I0lpRIBRJWCXf7ywpznxBDnMug+OemB3uYW4q3/BzQIxvHWUAA2EvWy6qpmhaKGl0Z9p3YrdM= 13, 67 -- Received: from MWHPR15MB1198.namprd15.prod.outlook.com (10.175.2.140) by 13, 67 -- MWHPR15MB1776.namprd15.prod.outlook.com (10.174.255.17) with Microsoft SMTP 13, 67 -- Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384) id 13, 67 -- 15.20.1404.20; 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Recent graduates and about-to-graduate PhD students are encouraged to apply.
-- Valery Ray (also with REFINE/UCONN) =================================== E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 MEO Engineering / PBS&T 290 Broadway, Suite 298 Methuen, MA 01844, USA www.partbeamsystech.com www.freudlabs.com
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Organization: Max Planck Florida Institute for Neuroscience
Title-Subject: [Filtered] CLEM Seminar at Max Planck Florida Institute -rescheduled
Message: This is an announcement for our re-scheduled event.
The Electron Microscopy Facility of the Max Planck Florida Institute for Neuroscience (Jupiter, FL) will host a MPFI-ZEISS labs-at-location partnership launch event on January 17th, 2019. The event includes a seminar focusing on the latest topics of Correlative Light-Electron Microscopy (CLEM), a demo for the new ZEISS Focal Charge Compensation module on the 3View-Gemini SEM300 system in the EM facility, and a partnership signing ceremony followed by a reception.
The event is open to all levels of researchers who are interested in utilizing the latest advances in electron microscopy. For more details and registration, please check our website; https://www.maxplanckflorida.org/events/other/mpfi-zeiss-labsatlocation-partnership-launch/
Registration is open until January 10th, 2019.
Please contact Naomi Kamasawa for more questions. naomi.kamasawa-at-mpfi.org
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Email: r.webster-at-unsw.edu.au Name: Richard Webster
Organization: UNSW, Sydney
Title-Subject: [Filtered] Position Available: Senior Research Fellow (TEM) at UNSW, Sydney
Message: We have a job opening for a Senior Research Fellow take a leading role in developing transmission electron microscopy, especially aberration-corrected transmission electron microscopy in the Electron Microscope Unit at UNSW, Sydney. This position is a 5 year appointment with options for renewal. All enquiries should be made to Prof Richard Tilley: r.tilley-at-unsw.edu.au. More details at: http://external-careers.jobs.unsw.edu.au/cw/en/job/495660/senior-research-fellow-transmission-electron-microscopy
Could you please forward this on to anyone you know that might be interested in the position?
Dr Richard F Webster
Research Associate, Electron Microscope Unit, Mark Wainwright Analytical Centre Rm B65, Chemical Sciences Building
UNSW Sydney NSW 2052 AUSTRALIA T: +61 (0)2 9385 6709 M: +61 (0)431 534 332 E: r.webster-at-unsw.edu.au W: unsw.edu.au
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My lab switched to ThermoFisher Pathfinder software from an older NSS. Still on the "uphill" part of the learning curve. My question concerns accessing the EDS map images in Spectral Imaging mode. How can I access the individual map images in a format that can be read into ImageJ? Can I get a TIFF image for example? Perhaps a silly question but right now I'm unable to solve this nut!
Thanks in advance.
Tom
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I manage a lab and have students inquire about that from our Oxford Aztec system now and then. I tried to persuade them that the vendors' software can do a lot. They may not want to reinvent the wheel.
If they insist, it is a fairly easy manner to export the Oxford maps into TIF format, although I think it a poor choice. (I don't know what PathFinder offers.) It does not retain the scaling of the data but contains 8-bit image values from 0-255 where the data has been stretched to fit. Many of my good maps do not have 100 counts per pixel. Most have far less. That pixel data was integrated over many channels, so you are giving up a lot of potential information going to one, rescaled number.
If you are still interested in processing the data on your own, I suggest you look into what others have already done, e.g., Lispix. It was developed at NIST for working with data cubes of spectral data. https://www.nist.gov/services-resources/software/lispix
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, December 17, 2018 7:07 AM To: Straszheim, Warren E [BIOTC]
-------- Forwarded Message --------
X-from: tomw-at-uidaho.edu
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Email: tomw-at-uidaho.edu Name: Tom Williams Organization: Univ of Idaho Title-Subject: [Filtered] Spectral Imaging Question Message: Greetings,
My lab switched to ThermoFisher Pathfinder software from an older NSS. Still on the "uphill" part of the learning curve. My question concerns accessing the EDS map images in Spectral Imaging mode. How can I access the individual map images in a format that can be read into ImageJ? Can I get a TIFF image for example? Perhaps a silly question but right now I'm unable to solve this nut!
i am doing a difficult search and googling leads me to directions I don't want. I hope you can help me with this. I want to use fluorescent microparticles as positive controls for an injection in skin samples. The skin samples are injected and then they are fixed, dehydrated and embedded in paraffin (using histoclear instead of xylol) using classical histological methodology. I want to be able to detect the fluorescence in paraffin sections without any further treatment. I don't want to detect the beads by immune detection or any labeling method on the paraffin sections. Optimally, the beads are 3-5µm in size and have a fluorescence spectrum similar to TRITC. Alternatively, I am looking for metallic beads (like gold or silver) with a diameter of several micrometers (unusual), which I can probably detect in bright field. Vendors are welcome to answer too.
Many thanks in advance!
Stephane
PS: a colored solution is no alternative, I want to detect particles
HI Stephane- What are your microparticles composed of? Make sure that they are not soluble in alcohols or histoclear before your process your tissues. If you have any doubts, I would suggest freezing the samples and cutting cryosections. Tissues are fixed, cryo-protected with sucrose and then frozen, so there are no solvents involved. I can send you and excellent protocol that gives very good histology (off list, since Nestor's filters will block attachments). Please let me know if you would like to try that.
Joyeux Noel et bonne annee.
Lee Cohen-Gould Microscopy Core Facility (212)746-6146
Weill Cornell Medicine Weill Cornell Medical College https://weillcornellmicroscopy.org/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__weillcornellmicroscopy.org_&d=DwMF-g&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=WGy9rNsJ0aftr-RZGhYifG7rQyiuTbXtsTp_tkrR4sU&s=L5S_-lX8w7d6DZZKPNbh9XZShx1oj5iwQGpenS97LzE&e=}
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Dear colleagues,
i am doing a difficult search and googling leads me to directions I don't want. I hope you can help me with this. I want to use fluorescent microparticles as positive controls for an injection in skin samples. The skin samples are injected and then they are fixed, dehydrated and embedded in paraffin (using histoclear instead of xylol) using classical histological methodology. I want to be able to detect the fluorescence in paraffin sections without any further treatment. I don't want to detect the beads by immune detection or any labeling method on the paraffin sections. Optimally, the beads are 3-5µm in size and have a fluorescence spectrum similar to TRITC. Alternatively, I am looking for metallic beads (like gold or silver) with a diameter of several micrometers (unusual), which I can probably detect in bright field. Vendors are welcome to answer too.
Many thanks in advance!
Stephane
PS: a colored solution is no alternative, I want to detect particles
You could use gold nanoparticles, for instance a BSA stabilized gold tracer, of any size and use silver enhancement on the section to enlarge their size for bright field observation. Very easy. Feel free to contact me off list, I will be happy to help.
Jan Leunissen Aurion - ImmunoGold Reagents
On 18/12/2018, at 23:47, nizets2-at-yahoo.com wrote:
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Dear colleagues,
i am doing a difficult search and googling leads me to directions I don't want. I hope you can help me with this. I want to use fluorescent microparticles as positive controls for an injection in skin samples. The skin samples are injected and then they are fixed, dehydrated and embedded in paraffin (using histoclear instead of xylol) using classical histological methodology. I want to be able to detect the fluorescence in paraffin sections without any further treatment. I don't want to detect the beads by immune detection or any labeling method on the paraffin sections. Optimally, the beads are 3-5µm in size and have a fluorescence spectrum similar to TRITC. Alternatively, I am looking for metallic beads (like gold or silver) with a diameter of several micrometers (unusual), which I can probably detect in bright field. Vendors are welcome to answer too.
Many thanks in advance!
Stephane
PS: a colored solution is no alternative, I want to detect particles
have you considered using ceramic microbeads instead of the metallic ones? I've never used them but they should come in the size range you desire.
Best,
Christian
On 18.12.18 11:49, nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } } i am doing a difficult search and googling leads me to directions I } don't want. } I hope you can help me with this. } I want to use fluorescent microparticles as positive controls for an } injection in skin samples. } The skin samples are injected and then they are fixed, dehydrated and } embedded in paraffin (using histoclear instead of xylol) using } classical histological methodology. } I want to be able to detect the fluorescence in paraffin sections } without any further treatment. } I don't want to detect the beads by immune detection or any labeling } method on the paraffin sections. } Optimally, the beads are 3-5µm in size and have a fluorescence } spectrum similar to TRITC. } Alternatively, I am looking for metallic beads (like gold or silver) } with a diameter of several micrometers (unusual), which I can probably } detect in bright field. } Vendors are welcome to answer too. } } Many thanks in advance! } } Stephane } } PS: a colored solution is no alternative, I want to detect particles } } ==============================Original } Headers============================== } 6, 32 -- From nizets2-at-yahoo.com Tue Dec 18 04:47:08 2018 } 6, 32 -- Received: from sonic303-3.consmr.mail.bf2.yahoo.com } (sonic303-3.consmr.mail.bf2.yahoo.com [74.6.131.42]) } 6, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } wBIAl8KD006468 } 6, 32 -- for {microscopy-at-microscopy.com} ; Tue, 18 Dec 2018 04:47:08 -0600 } 6, 32 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } d=yahoo.com; s=s2048; t=1545130339; } bh=zTNmmp9pg6tPeOLgqiBhW4xAu8QecNvKe3RkqKahsks=; } h=Date:From:To:Subject:References:From:Subject; } b=OzgT729VUOTXw39qmXp+w8WPvyfqDh//wbHJLR3eHnYdqk3rnY+/8ZTsyDadp7b9o/QaAVr5okvdo1SvLlxWGTLFqLT502evIktPOFXXyF8yU5tXJRXsD7bZDdH/F9qhZ2Mb45tR9BcyJ+/q+gnNlBgkCLbnwXa/5PFqu9AiHsqbF/pqxPio+cc+nvnW+Sdr31/HFVOLWSG7CVYah4tBE9cIENiUe1H/jvikooRwzWwzBzYnlsTtRa674pyfxiryLX6BbnF0+Dcrig+baO84GeZvpkNZc7gj3FqToYO9yQzIPL2XFC+f/vCrgj2/lu/qi7YIISszh49fXe/5/i6Z+g== } 6, 32 -- X-YMail-OSG: } VV0DnFEVM1l02kjVE2zL6FoaaXThJTzs88gT24NjCjidyA0BeoeBd.T1209EVez } 6, 32 -- } 4QusYu3cYRssn74OcqgNP0kuy2Ueq3lJfwnGxWQhFIE_wICRSj.PmBvqRTHDw_oBEg4PFE0dvnvA } 6, 32 -- } Xq4deRdAzg.tgww65Lvogs6Ezt1VqBmwcuw89dy0NWp4uB.PMUOw4b_xWcgkQmYjtUuBF5jAPPBI } 6, 32 -- } gUeBp1BofhY.1ck_cQO44IHhaqFg9dvZk2JM7lwE5XVwCdfhrv9EDdOtV4DfdyrgewaNh4koTkP4 } 6, 32 -- } aO82NxqBzHP4ymrfapeGmf0Y4zE0zofynYKOuvcaCnsIKBHH2UWk7WsnkSknd7S7OE7AzKBhtM95 } 6, 32 -- } xadVyqGr.XGov0.Vnn2YjeTnvdI1MV.5S_jOatK6taQ8l3KD3Merk3LPAt5tLU5fjsjpj9VcjJ6L } 6, 32 -- } XPExXpRoTqozYmqCnr01UALZpPPTTD.T7Trx1bg8T1QRJit0HMSloinF.U.ruBhKHJGvD70isMnk } 6, 32 -- } _OqDk9n85UBUSda.iehXTDyTUuiqFHRs5ucbPef4emW0yrnxcXSS7CiiXei0Z2W1w3kU00WfH09N } 6, 32 -- } 5SrL3F3CBFMpBQooqEWs2LR9XpbLLWi.j12JvRzTdQAutL2LDNoQ.tBJEk_X8C_8gHcvlZuC0nU4 } 6, 32 -- } t0PewiWQFK945meR4xoIkP2f0q_rPh8pcz9zdoG3_1drj_K2a4M1SPlr4l9bfbdJrhSobUDpELPB } 6, 32 -- } UJGiYmaDVm.jukhhHY38BsdfdDnNzM4a2Jx3Djiqf8Kd_vWR12ZiYnazZedwhsCMgJw7nrY_63M3 } 6, 32 -- } 3H2VV8_m1FE63SvWU4RpgOu8h1QZEyZtJM5GapsfqKO20snA8NIvy3h2VjcULkr6ZqkvQ.smfXWb } 6, 32 -- } rHFYkPyZBQtjzlaT8DhaJiTij6zO8AbOwrwGZdorLS9d.eiC.rX3NHS2pM5yzeREHQ.elnxFQviW } 6, 32 -- } liy02qCBBmvU.uV6nQ2KMjkhWjJiSTXi7BmoQVt5k26Gz8fXV5Nei.0HxxJMEbZx0i9VOmQB3asX } 6, 32 -- hdGOxsA090HZ2gcNr3cxa7ZfQisDvS7g5VpuNZnwOyQsSKmC_mMzV1XzBkg2m.A-- } 6, 32 -- Received: from sonic.gate.mail.ne1.yahoo.com by } sonic303.consmr.mail.bf2.yahoo.com with HTTP; Tue, 18 Dec 2018 } 10:52:19 +0000 } 6, 32 -- Date: Tue, 18 Dec 2018 10:52:17 +0000 (UTC) } 6, 32 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 6, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 6, 32 -- Message-ID: {1051087293.6020267.1545130337926-at-mail.yahoo.com} } 6, 32 -- Subject: Fluorescent beads surviving histological treatment } 6, 32 -- MIME-Version: 1.0 } 6, 32 -- Content-Type: text/plain; charset=UTF-8 } 6, 32 -- References: {1051087293.6020267.1545130337926.ref-at-mail.yahoo.com} } 6, 32 -- X-Mailer: WebService/1.1.12857 YMailNorrin Mozilla/5.0 } (Windows NT 10.0; Win64; x64) AppleWebKit/537.36 (KHTML, like Gecko) } Chrome/71.0.3578.98 Safari/537.36 } 6, 32 -- Content-Transfer-Encoding: 8bit } 6, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by } microscopy.com id wBIAl8KD006468 } ==============================End of - } Headers==============================
-- Christian Feldhaus, PhD Light Microscopy Facility MPI for Developmental Biology
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We own the W-filament version of this ESEM and I am attempting to identify owners that are experienced in servicing this because, service contracts are not supported here. I am trying to establish a network for collaboration in keeping this instrument operational.
Please contact me if you can offer assistance.
Thank you,
Rick
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