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From: microscopy.listserver-at-gmail.com
Date: Mon, 1 Jan 2018 08:55:50 -0600
Subject: [Microscopy] Administrivia: Happy New Year 2018

Contents Retrieved from Microscopy Listserver Archives
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The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2017, the ListServer delivered your messages to more than 4350 subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year you generated ~ 250+ Gbits of Email traffic and ~ 2.55 Million
Email messages were sent out this year by my tired and old little server. Up abit
from last year, but still a steady flow.


As usual you don't want to know how much Junk Mail and spam has been filtered out
far more than you might expect. Apologies to those that have problems with
my filters.


The complete Microscopy ListServer Archives for 2017-1994 (~ are on-line at

http://www.microscopy.com.

A couple of IMPORTANT reminders:

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Nestor
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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Jan 2018 21:57:05 -0600
Subject: [Microscopy] viaWWW: Biological TEM Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: jpshield-at-uga.edu

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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop

Message: This intensive, three-day workshop will provide a practical and basic theoretical
introduction to the Transmission Electron Microscope and biological sample preparation techniques.
Each day will consist of lecture, discussion and hands-on training led by GEM staff.
Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday through Wednesday, March 14-16, 2018, 8am-5pm each day (lunch is provided)

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login

Deadline: March 5, 2018
*****************
Past participants include Merck & Co., Connecticut State, Breathitt Vet Center, Univ. of Chicago,
Stowers Inst. for Medical Research, West Virginia State


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From: microscopy.listserver-at-gmail.com
Date: Sat, 6 Jan 2018 06:12:28 -0600
Subject: [Microscopy] viaWWW:Applications Scientist In Situ TEM - immedate opening at

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: ben-at-protochips.com


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Email: ben-at-protochips.com Name: Benjamin Jacobs

Organization: Protochips, Inc

Title-Subject: [Filtered] Applications Scientist In Situ TEM - immedate opening at Protochips

Message: Protochips has an immediate opening for a marketing applications scientist. Candidates must
have a strong background in TEM, and experience with in situ TEM such as heating, gas, liquid or
mechanical, is highly preferred.
Travel up to 40%, mostly domestic, but some international to Europe and East Asia.
Candidates must also be a US citizen or already be authorized to work in the US.
Position is located in the Raleigh/Durham area in North Carolina, and candidate must be willing to
relocate.

Please click the following link for more information:

http://www.protochips.com/news/marketing-applications-scientist/

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From: zaluzec-at-microscopy.com
Date: Sat, 6 Jan 2018 06:13:30 -0600
Subject: [Microscopy] viaWWW:Hiring - Research Associate - Electron Microscopy Technologist

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Email: walbrid-at-cshl.edu Name: Samantha Walbridge

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] Hiring - Research Associate - Electron Microscopy Technologist Position

Message:
Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
state-of-the-art Microscopy Shared Resource.

The individual must have extensive practical expertise in biological sample preparation for
transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on
knowledge of confocal and widefield fluorescence microscopy would also be a plus.

The candidate will help users design innovative experiments and they will carry out sample
preparation and imaging as well as assist in data interpretation.

Excellent verbal and written communication skills, ability to work with multiple users in a
supporting role, and ability to work independently and proactively with limited supervision are
essential. A Bachelor’s degree in biology or related discipline is required. One to three years of
experience working in a Microscopy Shared Resource is preferred.

How to Apply

Interested individuals should apply for this position via the CSHL careers website at
http://cshl.peopleadmin.com/postings/11688

Position Number 01779-R

Applicants should include a resume along with a description of their practical expertise and the
names as well as email addresses of 3 references.

Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized
internationally for its excellence in ground-breaking research programs in cancer, neuroscience,
plant biology, genomics, and bioinformatics and broad educational mission.

For more information about CSHL, please visit us at www.cshl.edu

CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and
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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Jan 2018 17:54:21 -0600
Subject: [Microscopy] viaWWW:Lehigh University Microscopy School 2018

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Email: SLC6-at-Lehigh.edu Name: Sharon Coe

Organization: Lehigh University

Title-Subject: [Filtered] Lehigh University Microscopy School 2018

Message: Now accepting registrations for the 48th Lehigh Microscopy School which will be held on the
campus of Lehigh University, Bethlehem, PA, June 3-8, 2018. All courses, lecturers, and
instrumentation will be together for what promises to be a phenomenal week! Course offerings
include: SEM and X-ray Microanalysis • Introduction to SEM and EDS for the New Operator • Focused
Ion Beam Instrumentation and Applications • Problem Solving: Interpretation and Analysis of
SEM/EDS/EBSD Data • Quantitative X-ray Micoanalysis • Problem Solving Using EDS and WDS Techniques •
Scanning Transmission Electron Microscopy: From Fundamentals to Advanced Applications. Register
and pay in full by April 13 for an early bird discount! Contact: Sharon Coe (sharon.coe-at-Lehigh.edu
or 610-758-5133). See www.Lehigh.edu/microscopy for registration form, prices, and details about
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From: microscopy.listserver-at-gmail.com
Date: Tue, 9 Jan 2018 07:09:14 -0600
Subject: [Microscopy] Fwd: Carbon coater and EMS 150T ES

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X-from: Guobin Hu {gb_hu-at-yahoo.com}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}



Hi microscopy subscribers,

We recently purchased an EMS T150 ES for carbon coating. Originally we had a standard chamber. The
coating was very inconsistent. We contact EMS and EMS sent us an extended height chamber and advised
us to control the coating by monitoring the FTM (Film Thickness Monitor) reading instead of by
editable profiles. It does make the coating more controllable. However, the results are still not
consistent.  For example, I coated carbon of 9 nm by FTM and also carbon of 12.5 nm by FTM. The 9 nm
thick carbon actually looked thicker than 12.5 nm. I would appreciate it if users here can share
your experience with me how to coat carbon with consistent and accurate thickness. On the other
hand, I would also appreciate it if you can make recommendations on carbon coaters.

Thanks,

Guobin


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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 09:54:08 -0600
Subject: [Microscopy] viaWWW:Need Fiji script to subtract background noise

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Email: driscollg2-at-winthrop.edu Name: Garrett Driscoll

Organization: Winthrop University

Title-Subject: [Filtered] Need Fiji script to subtract background noise

Message: Due to the large number of images and insufficient amount of ram, I am unable to load both
image sequences (~1900 per) on Fiji in order to use the standard "image calculator" to subtract my
images from the RFP (A) channel from GFP (B) channel to reduce background noise. I am looking for a
script that would allow me to subtract Image 1A - Image 1B = Image 1C, Image 2A - Image 2B = 2C...
etc. Ultimately, I am looking to create a Z-stack from my subtracted images to reduce background
noise to further analyze data.
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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:33:14 -0600
Subject: [Microscopy] Fwd: Water chiller for Hitachi SEM.

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X-from: Valery Ray {vray-at-partbeamsystech.com}

Hi Ravi,

Check cooling water requirements of the SEM (flow, dissipated power) and Google for compatible
chiller - there are literally hundreds if not thousands outlets selling them online, starting with
amazon.com and zoro.com and ending with ebay.com and aliexpress.com; chiller for running SEM could
be found with price tags below US$1,000.
Valery

Valery Ray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com


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*To:* vray-at-partbeamsystech.com
*Sent:* Thursday, January 11, 2018 9:49 AM
*Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.




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-------- Forwarded Message --------

X-from: McClintock, Joel {jmcclin-at-uky.edu}

Ravi,


This only needs a flow of 1-1.5 liters/minute. Not really very much. Short term, tap water supply
works fine. Risk of condensation and corrosion over time. Constantly running water, which no one
likes as well.


What does flow sensor in back of misroscope indicate is the flow? YOu can change the sensor point on
that flow gauge.


Pretty sure all you are cooling is the Diffusion pump. Think 700 Watt heater. I know the Neslab
CFT33 is sufficient. Specs for it are "950 Watts at 20C 3240 BTU/hr at 20C 817 Kcal/hr at 20C".
The CFT25 model may be not sufficient. The oldHaskris model RO33 will also work. You can find it's
specs. Think mostany chiller with similar specs will work.


Joel

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*Sent:* Thursday, January 11, 2018 10:56:19 AM
*To:* McClintock, Joel
*Subject:* [Microscopy] viaWWW: Water chiller for Hitachi SEM.



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-------- Forwarded Message --------

X-from: Crane, Dan - OSHA {Crane.Dan-at-dol.gov}


I have a Hitachi S3500-N and have used a Haskris R-033 water cooled chiller for it. It was the
recommended chiller by Hitachi. While possibly not the least expensive alternative, it has worked
well for us. You can always call Hitachi High Technologies America, Inc. service department and see
what they recommend.

We have used a variety of different chillers for our other microscopes and instruments, TEM, ICP,
and ICP-MS. Each has to be matched to the power load of the instrument so that they are adequate and
are not overworked so that they fail too soon.

The trick to know with SEM or TEM especially is that the chiller has to provide a very constant
temperature with no pulsation. Temperature is important for magnification stability and absence of
pulsation is essential for imaging and resolution. Not every chiller can provide either or both.
You may not have seen any problems with city water for pulsation or for rapid temperature variance
because of the nature of the source. However, when you go to a closed loop chiller, such problems
can surface and be problematic, really degrading images.

You will need to look at the installation section of the S3500-N instrument and it will give the
temperature and flow requirements for the tool. You can then go to Haskris, or any of the other
suppliers and see what they can do.

Be very careful of used chillers. Pumps and motor-pump interfaces have a lot of wear, might start to
pulse, and might simply give out with no warning. (Been there, done that.) Also, I just had a
compressor develop what can only be called a fistula between the cooling water and the Freon lines.
(We have relatively corrosive tap water.) This was an age-related failure.

I hope that this helps.
Dan Crane

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Thursday,
January 11, 2018 9:13 AM
To: Crane, Dan - OSHA




-------- Forwarded Message --------

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Ravi,

A Haskris chiller will work well - you can get these in air-cooled versions.
As for used … I don’t know. But it’s worth checking your university’s HVAC people. If they don’t
have one hanging around, there might be one available surplus.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab






Email: ravi.thakkar369-at-gmail.com Name: Ravi

Organization: Kansas State University

Title-Subject: [Filtered] Water chiller for Hitachi SEM.

Message: We have Hitachi S3500-N SEM. A chilled water from building supply was used to run the
machine, but now pressure got dropped and we are not getting sufficient flow rate to keep machine
running.I don't see chances of water pressure get increased.
1. Could you guys please suggest what could be the probable suggestion ?
2. Any suggestion, which recirculating chiller will work for Hitachi S3500-N SEM? 3. If you guys can
suggest any source from where we can get used chiller as our budget is limited.
Note : We do not have service contract.
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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:34:10 -0600
Subject: [Microscopy] viaWWW:Balzers BAF 301 freeze fracture consumables source

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X-from: randy-nessler-at-uiowa.edu

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Email: randy-nessler-at-uiowa.edu Name: Randy Nessler

Organization: University of Iowa

Title-Subject: [Filtered] Balzers BAF 301 freeze fracture consumables source

Message: I'm wondering if there is a source for the following items used in our Balzers BAF 301
freeze fracture apparatus:
Bal-Tec BU 020 023-T tungsten filaments
Platinum pellets BD481505 (Balzers part number)
Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number)
Balzers Union BD 484 058 carbon rods

Those part numbers are off some I have on hand, but I hope to add to my inventory.
Searching the internet, I saw the platinum inserts listed as BU 006 103-T, carbon rod for PT/C
evaporation as BU 006 101-T and the carbon rods as BU 006 115. Another listing was "carbon set" BU
020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in a publication rather
than vendor inventory.
Thanks for any help in advance,
Randy


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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:35:19 -0600
Subject: [Microscopy] viaWWW:Seeking Applications Scientists for In Situ TEM in China

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Email: ben-at-protochips.com Name: Benjamin Jacobs

Organization: Protochips, Inc

Title-Subject: [Filtered] Seeking Applications Scientists for In Situ TEM in China

Message: Protochips has immediate openings for a applications scientists located in China.
Candidates must have a strong background in TEM, and experience with in situ TEM such as heating,
gas, liquid or mechanical, is highly preferred.
Travel up to 50%, mostly within China, but some international to Europe and the United States for
training and conferences.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jan 2018 18:41:37 -0600
Subject: [Microscopy] viaWWW:Postdoctoral Position at ORNL on advanced and in situ STEM

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Email: unocicka-at-ornl.gov Name: Kinga Unocic

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] Postdoctoral Position at ORNL on advanced and in situ STEM

Message: There is a postdoctoral position open at ORNL in the Materials Science and Technology
Division on Advanced and in situ STEM (nanomechanics and catalysts)

Qualified candidates can apply through the following link:
https://recruiting.ornl.gov/sap/bc/webdynpro/sap/zornl_hrrcf_c_post_doc?sap-language=EN&sap-client=010#

then search for the "Postdoctoral Research Associate in Advanced STEM Characterization / NB50650181"
Posting


Purpose
We are seeking a Postdoctoral Research Associate to focus on the mechanical behavior of materials at
the nanoscale level,
investigate the morphological changes of catalysts during their “life-cycle,” and work with a team
to develop advanced
electron microscopy analysis methods. While this position is based in our Electron Microscopy (EM)
group, you'll collaborate
with a diverse group of experimentalists, theorists, and data scientists across different
organizations within Oak Ridge
National Laboratory (ORNL). This position resides within the Materials Science and Technology
Division (MSTD), Physical
Sciences Directorate at ORNL.

OVERVIEW: As a postdoc, you will support the two projects indicated above while advancing data
acquisition and data
analysis methods for electron microscopy. The mechanical research will be directed toward the
correlation of composition
and crystallographic orientation with mechanical response at the nanoscale level using in situ
nano-compression and
scanning transmission electron microscopy (STEM). The catalytic research will focus on analyzing
structural changes from
the atomic to mesoscale within catalysts and correlating structure, porosity, and compositional
changes with applied
synthesis and other applied treatments/reactions. To do this, you’ll use aberration-corrected
scanning transmission electron
microscopy (STEM) and imaging and analytical microscopy techniques, such as electron energy loss
spectroscopy (EELS)
and energy X-ray dispersive spectroscopy (EDS). You'll also work with a multidisciplinary research
team to develop electron
microscopy characterization techniques suitable for the catalyst and quantitative data analytic
methods to determine the
factors that govern catalytic processes at the atomic level.

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From: wij.muss-at-aon.at
Date: Sat, 13 Jan 2018 15:53:07 -0600
Subject: [Microscopy] Re: Balzers BAF 301 freeze fracture consumables source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings!
Dear Randy,

this is what I found:
ok: page document from 2009.... not knowing whether Stacey Kirsch has
ready or in stock your requested consumables
https://www.emsdiasum.com/microscopy/products/brochures/2009/ems2_09.pdf
p.8.:
Our facility is equipped to handle the following Manufacturers:
} } Balzers/Baltech
email: sgkcck-at-aol.com or stacie-at-ems-secure.com

or:
http://www.highland-scientific.com/balzersbn.html
[NB: ok, This page last updated at 11.13 on Thursday, 14 November 2013]
Contact details:
Highland Scientific
Unit 20, Bedford Business Centre
Mile Road
Bedford
MK42 9TW
England
Tel. + 44 (0)1 234 216 636
Fax. + 44 (0)1 234 271 991
Sales-at-Highland-Scientific.com


As of 25.02.2008: LEICA Microsystems buys / bought BAL-TEC in
Liechtenstein (sorry only in German)
http://www.chemie.de/news/78525/erneuter-firmenerwerb-durch-leica-microsyste
ms-bal-tec-in-liechtenstein.html
so it might be at least possible to find the rep-site of LEICA Microsystems
IOWA or USA and ask them about...


Last but not least (I was able some years ago also to help another
US-colleague to order parts and consumables for former BALZERS - then
BAL-TEC products now BALTIC PRAEparation.e.K.:
Cf: http://www.baltic-praeparation.de/ (unfortunately I found only a
German webpage...
Claudia Kster
Baltic Prparation e.K.
Koppelheck 34b
D-24395 Niesgrau
Telefon: 0 46 43 / 18 65 43
Telefax: 0 46 43 / 18 65 54
Mobil: 0172 / 626 44 55
Email: baltic.praeparation-at-t-online.de

I've written to them using their the php form and asked a preliminary
question for you...and I am sure they will answer your request written in
English...

Best wishes to you and yours,
Wolfgang

=======================================================
MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA

Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

Former Secretary and (until June2017) Board Member of the

SCUR
{The Society for Cutaneous Ultrastructure Research}
The Skin Imaging Society { www.scur.org }
====================================================================


Von: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com]
Gesendet: Freitag, 12. Januar 2018 02:03
An: wij.muss-at-aon.at
Betreff: [Microscopy] Balzers BAF 301 freeze fracture consumables source
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Email: randy-nessler-at-uiowa.edu Name: Randy Nessler
Organization: University of Iowa
Title-Subject: Balzers BAF 301 freeze fracture
consumables source
Message:

I'm wondering if there is a source for the following items used in our
Balzers BAF 301 freeze fracture apparatus:
Bal-Tec BU 020 023-T tungsten filaments
Platinum pellets BD481505 (Balzers part number)
Hollow end carbon rods to hold Pt pellets BD484055 (Balzers part number)
Balzers Union BD 484 058 carbon rods

Those part numbers are off some I have on hand, but I hope to add to my
inventory.
Searching the internet, I saw the platinum inserts listed as BU 006 103-T,
carbon rod for PT/C evaporation as BU 006 101-T and the carbon rods as BU
006 115. Another listing was "carbon set" BU
020 077-T, and Pt-C set BU 020 075-T. Unfortunately, these were citations in
a publication rather than vendor inventory.
Thanks for any help in advance,
Randy

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From: CGorman-at-hookecollege.com
Date: Mon, 15 Jan 2018 11:53:08 -0600
Subject: [Microscopy] Scanning Electron Microscopy Training

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Greetings,

Hooke College of Applied Sciences, located in Westmont, IL, is offering a Scanning Electron Microscopy short course March 5-9, 2018.

In addition to lectures, this course emphasizes hands-on training using state-of-the-art equipment.

For further SEM training details and registration information, please follow the link below:

https://www.mccrone.com/scanning-electron-microscopy-course

Best regards-
__________________________________________________
Chris



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From: microscopy.listserver-at-gmail.com
Date: Tue, 16 Jan 2018 08:54:07 -0600
Subject: [Microscopy] viaWWW:Help with ASPEX 3025 SEM

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Email: jdykes-at-wvc.edu Name: Jeff Dykes

Organization: Wenatchee Valley College

Title-Subject: [Filtered] Help with ASPEX 3025 SEM

Message: Hello All,

My department just received a Scanning Electron Microscope and we are looking for some assistance.
We are in need of:

-An operators manual that could be copied and sent to us. It is for a ASPEX 3025 SEM.
-Guidance or protocols to prepare (fix) samples such as plants, bacteria, insects.
-Any other documents, tips, etc.

Thank you,

Jeff Dykes
Science Faculty
Wenatchee Valley College
116 West Apple
Omak, WA 98841 (USA)

jdykes-at-wvc.edu
509 422-7876

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jan 2018 07:22:28 -0600
Subject: [Microscopy] viaWWW:Postdoc - Advanced TEM of Steel/Thin Films

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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoc - Advanced TEM of Steel/Thin Films

Message: Postdoc position in Advanced Microscopy of Steel Microstructure and Thin Films - Position
Available Now.

A postdoctoral position is available in the performance of two distinct projects. The researcher
will use the state-of-the-art aberration-corrected TEM's at the Canadian Centre for Electron
Microscopy (ccem.mcmaster.ca) located at McMaster University, Hamilton Canada. The CCEM is a
world-class imaging facility and is equipped with 4 TEM's, including two aberration-corrected
microscopes (FEI Titans).
The first project is to study precipitation mechanisms of Nb-rich steels after hot rolling. This
work requires knowledge of chemical imaging, diffraction methods, and an understanding of strain
measurement. The second project is the study of the thin film growth process, from 2-D materials up
to ALD-grown films. This requires high-resolution imaging (TEM and STEM), EELS, and EDS.

The postdoc will be part of the Bassim research group, with a strong collaboration with Prof.
Zurob's research group.

Applicants should have a strong background in electron microscopy, preferably with
aberration-corrected (S)TEM experience. Additional background in metallurgy or thin films is a
bonus. Ability to work in a team, mentor graduate students, as well as excellent verbal and written
communication skills are expected.

McMaster University is located in Hamilton, Ontario, within a 45 minute drive from downtown Toronto,
which a world class city and 45 minute drive to Niagara Falls.

To apply, please send a cover letter, curriculum vitae including references, and 1-2 relevant
published papers to the e-mail below.

Nabil Bassim
Associate Professor
Department of Materials Science and Engineering, JHE 258
McMaster University
1280 Main Street West
Hamilton, ON L8S 4L8
CANADA
nbassim.mcmaster.ca
bassimn-at-mcmaster.ca

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jan 2018 07:23:09 -0600
Subject: [Microscopy] viaWWW:Solid State X-ray Spectrometry at 50 Years: Session A11

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Email: paul.carpenter.epma-at-gmail.com Name: Paul Carpenter

Organization: Washington University

Title-Subject: [Filtered] Solid State X-ray Spectrometry at 50 Years: Session A11 Microscopy &
Microanalysis 2018

Message: Dear Microscopists and Microanalysts,
It is our pleasure to inform you of: Solid State X-ray Spectrometry at 50 Years, session A11 at the
2018 Microscopy & Microanalysis Meeting, which will be held Aug. 5-9, 2018 in Baltimore, MD.

This session will concentrate on the diverse advances in hardware, software, and applications of
energy-dispersive spectrometry in the last 50 years.

We are particularly interested in applications from the biological and materials communities,
especially from students and young scientists.

The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018:
https://www.microscopy.org/MandM/2018/

Solid State X-ray Spectrometry at 50 Years

Session chairs: Paul Carpenter, Edward Vicenzi, Kate Burgess, Nicholas Ritchie

50 years ago, Fitzgerald, Keil, and Heinrich published the first results obtained from an
energy-dispersive x-ray spectrometer (EDS) in Science. From identification of unknown materials, to
compositional mapping and quantitative microanalysis, EDS has advanced our understanding of an
enormous range of materials and is used worldwide in microanalysis and microscopy laboratories. The
symposium will link historical and technical developments of solid state x-ray instrumentation, data
processing, applications, and emerging detection systems. Perspectives on the developments in EDS
from a technological and educational perspective will be featured, including invited and contributed
presentations from the inventor, vendor, and scientific communities.

o State-of-the-art solid state x-ray detector instrumentation
o Soft x-ray and light element analysis in the field emission SEM
o Nano to atomic resolution microanalysis by STEM
o Quantitative analysis methods - limits and limitations
o Spectrum imaging and data processing
o Complementary methodologies including micro-XRF, combined WDS-EDS, XRD, and EBSD

Paul Carpenter, Washington University

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jan 2018 07:23:57 -0600
Subject: [Microscopy] viaWWW:Lorentz microscopy - position

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Email: klemari-at-fzu.cz Name: Mariana Klementova

Organization: Institute of Physics of the Czech Academy of Sciences

Title-Subject: [Filtered] Lorentz microscopy - position

Message: Dear colleagues,

we are currently looking for a microscopist who would be able to introduce Lorentz microscopy to our
group at the Institute of Physics. We have a Tecnai F20 X-twin with a Lorentz lens. We have got
funding for the position for 6 months. The position will be available from June 1, 2018.

If you are interested please contact me by January 31, 2018.

With best regards,

Mariana Klementova

************************************************
Mariana Klementova
************************************************
Institute of Physics of the CAS, v.v.i.
Na Slovance 1999/2
CZ-182 21 Praha 8
Czech Republic
************************************************
tel. : +420 - 266 05 2612
email: klemari-at-fzu.cz
************************************************

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From: microscopy.listserver-at-gmail.com
Date: Thu, 18 Jan 2018 07:55:36 -0600
Subject: [Microscopy] viaWWW:Entry level microscope sales - San Francisco and Seattle

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Email: kandi-at-isearchbio.com Name: Kandi Williams

Organization: K.L. Williams & Associates

Title-Subject: [Filtered] Entry level microscope sales - San Francisco and Seattle

Message: We are currently working with a major manufacturer of laboratory microscopes and related
optical products to assist them in filling a San Francisco and a Seattle based entry level
microscope sales positions. We are looking for a young, energetic scientist with a year or two of
post doc experience, or several years post graduate lab management experience, with heavy confocal
experience – using, teaching, troubleshooting. This would be an excellent first step off the bench
into industry.
The company will provide excellent training and mentorship and a solid career path. First year
salary package is anticipated to be around $100k plus the company provides a monthly car allowance,
covers all business and travel expenses and has an excellent benefits package. Overnight business
travel for both territories is low, maybe 2 to 3 nights/month, primarily for meetings and training.
Please note that the company is unable to provide any sort of visa sponsorship at this time. Take care,
Kandi Williams

K.L. Williams & Associates
P.O. Box 5421
Auburn, CA 95604-5421
(530) 885-3693
kandi at isearchbio.com


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From: Thomas.Kremer-at-sgs.com
Date: Fri, 19 Jan 2018 14:13:48 -0600
Subject: [Microscopy] SEM - Using a Wig-L-Bug grinder

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Hello,
I recently purchased a Wig-L-Bug grinder/mixer, an agate vial and an adapter to hold the vial. I am at a loss as to how the vial is held by the adapter and the adapter certainly does not fit on the arms of the grinder/mixer. I made three attempts at contacting the manufacturer's technical support but to no avail. Hence this plea for help. If any of you have this setup, I appreciate your input. I can share photos of the parts if that would help.

BTW, the purpose for the agate vial is for grinding ashed materials that often contain silicates.

Tom Kremer
SGS-IPS Testing
Appleton, WI
Information in this email and any attachments is confidential and intended solely for the use of the individual(s) to whom it is addressed or otherwise directed. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of the Company. Finally, the recipient should check this email and any attachments for the presence of viruses. The Company accepts no liability for any damage caused by any virus transmitted by this email. All SGS services are rendered in accordance with the applicable SGS conditions of service available on request and accessible at http://www.sgs.com/en/Terms-and-Conditions.aspx


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From: microscopy.listserver-at-gmail.com
Date: Sun, 21 Jan 2018 22:31:09 -0600
Subject: [Microscopy] viaWWW:Vac check connection failure, Zeiss Leo SEM

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Email: mplattsc-at-gmail.com Name: Michael Platt

Organization: University of South Carolina

Title-Subject: [Filtered] Vac check connection failure, Zeiss Leo SEM

Message: I have been working on this issue, along with an L-REM failure off and on for a while and
have gotten nowhere. I have been concentrating on the L-REM failure but now I want to look harder
into trying to solve the Vac Check Connection fail. Those failures show up during the power up
initialization. First, what does that really mean? Not that it has anything to do with the failure
but the backing pump and turbo pump both come on and I believe the turbo comes up to speed, solid
green light on the controller. The vacuum gauge's green lamp is also on.
Regarding previous suggestions I have check every fuse I could find, all good. All fiber optic
cables appear to be OK on the L-REM and associated units. Tests indicate the power supply failures
on the EO and PU boards but where exactly are they as I could find only numeric id's for all the PC
boards.

Suggestions?

Michael Platt


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From: microscopy.listserver-at-gmail.com
Date: Mon, 22 Jan 2018 09:32:26 -0600
Subject: [Microscopy] viaWWW:The Rockefeller University seeks Electron Microscopy

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Email: kuryu-at-rockefeller.edu Name: Kunihiro Uryu

Organization: The Rockefeller University

Title-Subject: [Filtered] The Rockefeller University seeks Electron Microscopy Specialist

Message: Message: Electron Microscopist, The Rockefeller University, New York, NY

The Rockefeller University, a premier biomedical research institution, seeks a Research
Support-at-Associate or Specialist to join our Electron Microscopy Resource Center (EMRC).

The EMRC provides state-of-the-art electron microscopy support for analysis of a wide variety of
biological samples, including viruses, bacteria, insects, animal tissue as well as cultured cells
and isolated cellular components for structural analyses or immuno-electron microscopy. The EMRC is
equipped with three transmission electron microscopes, a conventional and a serial block-face
imaging scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit.
(http://www.rockefeller.edu/emrc/)

The Research Support Associate/Specialist will participate in all of the EMRCfs daily operations,
including maintenance, upkeep and use of the electron microscopes and associated equipment, ordering
supplies, interacting with vendors, and administrative support for office duties, including center
billing. The position also entails specimen preparation, including negative staining, ultrathin
sectioning, and immunolabeling, operation of the microscopes and associated equipment, training
users, as well as consulting scientists on the design of experiments, data processing/analysis,
interpretation of results, and informing users on the latest methodology through familiarity with
relevant literature.

The successful candidate will have an M.S./Ph.D. degree or equivalent background in biology,
bioengineering or a related field and must have a minimum of 5 years of hands-on experience in
electron microscopy. A strong background in computation would be a plus. Must have strong
communication skills, and the ability to work collaboratively in a team as well as independently on
a wide variety of research projects. Must be detail-oriented, focused, and highly motivated.

We offer a competitive salary, comprehensive benefits, and a collegial work environment. To apply to
this job, click the following URL, click on 'staff opportunitiesf and enter keywordeIRC20449f or
eElectron Microscopistf: http://www.rockefeller.edu/hr/career.php

The Rockefeller University is an Affirmative Action/Equal Employment Opportunity/VEVRAA employer.

Regards,
Hiro
------
Kunihiro Uryu, Ph.D., Research Assistant Professor
Director of Electron Microscopy Resource Center (EMRC)
RRB Rm120
The Rockefeller University
1230 York Ave., Box 230
New York, NY 10065


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From: microscopy.listserver-at-gmail.com
Date: Tue, 23 Jan 2018 07:46:28 -0600
Subject: [Microscopy] viaWWW:Spectroscopic and Imaging Studies in Heritage Science

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Email: lamt-at-si.edu Name: Thomas Lam

Organization: Smithsonian Museum Conservation Institute

Title-Subject: [Filtered] P08 Spectroscopic and Imaging Studies in Heritage Science

Message: It is our pleasure to inform you there will be a Spectroscopic and Imaging Studies in
Heritage Science symposium, session P08 at the 2018 Microscopy & Microanalysis Meeting, which will
be held Aug. 5-9, 2018 in Baltimore, MD.

The MSA website link to submit a paper is now active. The deadline for submission is Feb. 15, 2018:
https://www.microscopy.org/MandM/2018/

P08 Spectroscopic and Imaging Studies in Heritage Science

The application of microscale and nanoscale characterization techniques to the examination of
cultural heritage materials has greatly enhanced our understanding of the processes that formed, and
subsequently transformed those materials to their present state. Understanding the chemistry and
morphology of heritage materials from the macro/mesoscopic scale to the microscale is of critical
importance for our increasingly deeper levels of knowledge of the interaction between objects and
their environment. This symposium will include invited and contributed presentations from students,
conservators, conservation scientists, researchers, and those from other disciplines who have an
interest in the preservation of cultural heritage.

o Emerging methods for microscale and nanoscale examination in culture heritage
o Case studies on historic and prehistoric materials
o Non-invasive and minimally-invasive imaging and analysis methods
o Kinetics of light fastness using microfadeometry
o Synchrotron- and neutron-based studies
o New approaches to protective coatings for heritage objects
________________________________________

Students, postdocs, and technical staff
All full-time students enrolled at accredited academic institutions and all full-time postdoctoral
fellows are eligible for meeting award support. High school, undergraduate, and graduate students
are encouraged to apply. Applicants are not required to be members of the sponsoring societies.
Additionally, full-time technologists are eligible for support as well if the applicant is a member
of the sponsoring society, current in his or her dues for the year of the meeting.

We look forward to seeing you at the Baltimore inner harbor next year!
On behalf of the symposium organizers,

Edward P. Vicenzi (Smithsonian Institution)
John Mansfield (University of Michigan)
Thomas Lam (Smithsonian Institution)


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From: microscopy.listserver-at-gmail.com
Date: Fri, 26 Jan 2018 08:51:29 -0600
Subject: [Microscopy] viaWWW:NCSM Spring Meeting in Pleasanton, CA

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Email: info-at-ncsmicroscopy.org Name: Roseann Csencsits

Organization: Northern California Society for Microscopy

Title-Subject: [Filtered] NCSM Spring Meeting in Pleasanton, CA

Message: Northern California Society for Microscopy announces its spring meeting on March 27 at the
Zeiss Customer Center in Pleasanton, CA. MSA guest tour speaker is: Sara Miller, PhD. of Duke
Medical Center.
Presenting: “Emerging Diseases and Microscopy”

More info at: ncsmicroscopy.org

RSVP: info-at-ncsmicroscopy.org

We hope you will attend.


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From: ajhall-at-prairienanotech.com
Date: Mon, 29 Jan 2018 12:36:10 -0600
Subject: [Microscopy] Re: Ask a Microscopist - embedding pigments & powders for microtomy

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***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
**any reply should go directly to the poster**
as well as to the list.
Using the "reply" function in your email does *not* send your answer
to the person asking the question.
Please copy their email address from their question.
***********************************************************************************





Name: Janine Hernandez
School: San Joaquin Delta College
Grade/Education Level: Undergraduate
Location: Stockton, CA
Email: janine.hernandez182-at-gmail.com

(Listserve - I apologize - I think I did the reply incorrectly at first)

Hi Janine,

Powders and pigments likely do not require encapsulation. It's usually
much easier to place them on a supporting grid directly for imaging.
This of course assumes that the size of the powder or pigment is small
enough for electron transmission. Often it's worth it just to check
incase it is possible; which makes sample prep fairly easy.

There are a few methods that could be used to prepare these- one that
I've had some success with fine powders in the past is as follows: place
an amount of the powder into isopropanol, ultrasonicate to break up
agglomerations, dip a holy-carbon grid into the liquid, pull it out, and
allowing the grid to air dry. If there was a small enough amount, but
enough to be finely dispersed in the alcohol, you will pick up some on
the grid. This grid, when dried, can then be imaged with the powders
suspended on the holy-carbon. It may take a few trials to get enough
from the alcohol suspension. Another method that has worked for me in
the past (assuming the powder is well separated) is a very light dusting
of the powder mid-air above the grid (allowing some of the powder to
fall down onto the grid on the table). This one is less likely to obtain
separated powders that are small enough to image, but I have seen some
success with it as well in the past. (Use of a clean fine artist paint
brush to flick the powder into the air above the grid may work here.)
Please be careful not to allow the alcohol with the powder or the powder
in air to get on your skin or to be breathed in. Some solvents (acetone
notably) allows chemicals to enter through the skin, and we are learning
that nanopowders are not healthy for you to breathe in as well.

I hope this is helpful. You'll likely get a number of great answers
from the list-serve and I'm looking forward to learning from their
answers also!

Wishing you a grand time exploring small worlds!
-Allen J. Hall
Prairie Nanotechnology
www.prairienanotech.com

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From: korinek-at-mcmaster.ca
Date: Mon, 29 Jan 2018 15:19:14 -0600
Subject: [Microscopy] Job Opening: Senior Postdoctoral Fellow in the Canadian Centre for

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Senior Postdoctoral Fellow in the Canadian Centre for Electron
Microscopy

The Canadian Centre for Electron Microscopy (CCEM) is a national
facility located at McMaster University, in Hamilton, ON, Canada.
The CCEM operates four TEMs, including two FEI Titan TEMs, equipped
with image
and probe correctors and monochromators, as well as a Quantum GIF
equipped with
K2 direct electron detector for spectroscopy.

We are seeking a skilled and motivated researcher to perform a variety
of
imaging and analysis experiments supporting the needs of academic and
industrial
users, including consulting, planning of experiments, data
processing and interpretation. An important role of this position is
the training of users on the
instruments.

Candidates should have a PhD in Physics, Material Science/Engineering,
Chemistry or
a related field with demonstrated hands-on experience
using transmission electron
microscopes and EDS/EELS, preferrably Titan TEMs. Strong communication
skills and the ability to develop successful data acquisition and
processing strategies will be essential in this role. Experience in
data processing and scripting in Python or Matlab, as well as a
demonstrated
track record of research and interactions with users would be an asset.

To apply, please send your cover letter and resume to korinek-at-mcmaster.
ca


--
Dr. Andreas Korinek

Manager

Canadian Centre for Electron Microscopy
Brockhouse Institute for Materials Research
McMaster University
1280 Main Street West, Hamilton ON Canada, L8S 4M1
phone: +1 905-525-9140 ext 20400


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From: microscopy.listserver-at-gmail.com
Date: Tue, 30 Jan 2018 08:05:06 -0600
Subject: [Microscopy] viaWWW: Zeiss 10C available

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Email: psicurello-at-ucsd.edu Name: Paula Sicurello

Organization: UCSD

Title-Subject: [Filtered] Zeiss 10C available

Message: Please post for me this Zeiss 10C that we are giving away.

Hello Everybody,

Free to a good home.

Is anyone interested in a vintage Zeiss 10C? This scope is in working condition and has been
lovingly maintained. It comes with a side mounted Gatan ES1000W camera. The purchaser will be
responsible for the costs associated with the disassembly and removal.

Please contact me if you are interested.

Sincerely,

Paula Sicurello, HTL (ASCP)

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

psicurello-at-ucsd.edu
P: 619-583-2872

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health
200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872


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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Jan 2018 09:10:45 -0600
Subject: [Microscopy] viaWWW:Fluorescent Microspheres

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Email: holtzcll-at-mail.nih.gov Name: Lynne Holtzclaw

Organization: NIH

Title-Subject: [Filtered] Fluorescent Microspheres

Message: Does anyone have a proven protocol for coating #1.5 coverglass with a layer of fluorescent
microspheres? (Tetraspeck, 0.1)

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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Jan 2018 09:11:26 -0600
Subject: [Microscopy] viaWWW: ETEC Autoscan SEM circuit diagrams needed

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Email: cjbray-at-es.utoronto.ca Name: Colin Bray

Organization: Earth Science, Univ. of Toronto

Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed

Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the
vacuum system driver board. he has circuit diagrams for most of the other units but naturally, not
the one he needs. Can anyone help?

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From: sergei2-at-ornl.gov
Date: Wed, 31 Jan 2018 10:54:42 -0600
Subject: [Microscopy] Piezoresponse Force Microscopy tutorial - integrated slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues - to commemorate the decade of PFM conferences, I have
made the integrated set of slides (~350) on the principles and
applications of piezoresponse force microscopy and spectroscopy based on
tutorials on ~20 PFM workshops and conferences over last decade.

There are 7 parts, including:
1. Introduction to PFM and Nanoscale electromechanical phenomena
2. Contact mechanics and image formation in PFM
3. Cantilever dynamics in PFM
4. PFM of ferroelectric materials and local polarization switching
5. Switching spectroscopy piezoresponse force microscopy
6. Advanced time- and voltage spectroscopies in PFM
7. PFM in polar and nonpolar liquids

If interested, please contact me directly at sergei2-at-ornl.gov
{mailto:sergei2-at-ornl.gov} . Also, please forward this information to your
colleagues interested in PFM.

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Feb 2018 15:01:25 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: ETEC Autoscan SEM circuit diagrams

Contents Retrieved from Microscopy Listserver Archives
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Ken Converse used to support ETECs, the bits of contact info I have are:

QUALITY IMAGES
Servicing Scanning Electron Microscopes
Since 1981
474 So. Bridgton Rd.
Bridgton, ME  04009
207-647-4348
Fax 207-647-2688
kenconverse-at-qualityimages.biz {mailto:kenconverse-at-qualityimages.biz}
qualityimages.biz

Quality Images, /Ken Converse/, Maine, from NC to ME to OH, SEM, Amray//Etec//Any, (717) 456-5491

Good luck!
Valery
Valery Ray
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*To:* vray-at-partbeamsystech.com
*Sent:* Wednesday, January 31, 2018 9:08 AM
*Subject:* [Microscopy] viaWWW: ETEC Autoscan SEM circuit diagrams needed




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Email: cjbray-at-es.utoronto.ca {mailto:cjbray-at-es.utoronto.ca} Name: Colin Bray

Organization: Earth Science, Univ. of Toronto

Title-Subject: [Filtered] ETEC Autoscan SEM circuit diagrams needed

Message: A colleague has an ETEC Autoscan SEM with an EDS and is need of a circuit diagram for the
vacuum system driver board.  he has circuit diagrams for most of the other units but naturally, not
the one he needs.  Can anyone help?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Feb 2018 15:02:44 -0600
Subject: [Microscopy] viaWWW:Beads size and intensity

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Email: tstern-at-princeton.edu Name: Tomer Stern

Organization: Princeton University

Title-Subject: [Filtered] Beads size and intensity

Message: Hi all,

I'm using a MuVi-SPIM, similar to the Luxendo model.

To register the different views and also to infer the PSF prior to deconvolution I'm using beads,
which are scattered around the sample - inside the agarose - and are being scanned at the same time
as the sample.

I have two questions:

1. Is the intensity of the beads relative to the intensity of the marker inside the tissue important
for the inference of the PSF? Meaning, is it better to use beads with intensities that are similar
to the sample, or should their intensity be higher / lower / it doesn't really matter?

2. As far as I know the preferred diameter of the bead is one that is a bit smaller than the size of
the voxel in the XY plane, right? if so, then if I know that I'm going to use a 2x2 binning simply
by averaging every 2x2 block in each slide, should I still use the same size of beads, or should it
match the new size of the 'binned' - enlarged - pixels?


Thanks in advance!
Tomer


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From: kbustillo-at-lbl.gov
Date: Thu, 1 Feb 2018 19:49:47 -0600
Subject: [Microscopy] Scientific Engineering Associate position at NCEM (Berkeley, CA)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Berkeley Lab’s Molecular Foundry Division has an opening for a
Scientific Engineering Associate or Sr. Scientific Engineering
Associate. The Molecular Foundry is a Department of Energy-funded
nanoscience research facility that provides users from around the
world with access to cutting-edge expertise and instrumentation in a
collaborative, multidisciplinary environment. As one of the seven
facilities in the Molecular Foundry, The National Center for Electron
Microscopy (NCEM) serves a broad user community in materials science
by supplying unique instrumentation, expert advice, assistance, and
training in advanced electron microscopy techniques.

Diversity, equity, and inclusion are core values at the Molecular
Foundry. Therefore, we seek candidates whose research, past
employment, or service has prepared them to contribute to our
commitment to diversity and inclusion. Our excellence can only be
fully realized by staff who share our commitment to these values.
Successful candidates for our staff position will demonstrate either
potential to contribute or evidence of a commitment to equity and
inclusion.

This position provides highly specialized expertise, consultation, and
contributions to project process and knowledge in support of in situ
transmission electron microscopy (TEM) experimentation and TEM sample
preparation. This includes the development and optimization of
techniques for preparing high resolution TEM samples from a wide
variety of materials and for in situ TEM investigations, user
assistance and training, technical proposal evaluation, and
collaborations on scientific projects. The position is responsible for
scheduling, maintenance, operation and technical development of the
sample preparation laboratories and in situ TEM experimental
equipment. Will provide resolution to a diverse range of moderately
complex technical problems and participate in, and in some cases lead,
experimental methodology development and project execution.
Classification will depend upon the applicant's level of skills,
knowledge, and abilities.

For further details and to apply please go to the following link:

http://m.rfer.us/LBLYNuJJ


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From: microscopy.listserver-at-gmail.com
Date: Tue, 6 Feb 2018 07:26:12 -0600
Subject: [Microscopy] viaWWW:Gatan dimple grinder

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Email: jflaci-at-ms.sapientia.ro Name: Lszl Jakab-Farkas

Organization: Sapientia EMTE

Title-Subject: [Filtered] Gatan dimple grinder

Message: Hello,
I'm from a small university in Transsylvania,
we are running a microscopy lab with a vintage Jeol 100U and an JSM-5200.

We would need in our laboratory for sample preparation a Gatan Dimple
Grinder.

Can You point me to a reliable source for
cheaper second hand equipment?

Cheers

JFL



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From: microscopy.listserver-at-gmail.com
Date: Wed, 7 Feb 2018 07:32:30 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW:Postdoctoral_Fellow_=c2=96_In_situ_cryo-EM?=

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Email: carsten.sachse-at-embl.de Name: Carsten Sachse

Organization: EMBL Heidelberg

Title-Subject: [Filtered] Postdoctoral Fellow – In situ cryo-EM

Message: Dear list members,

In my lab, a position of in situ cryo-EM in the area of structural cell
biology is available. I am still looking for a candidate with experience
in cryo-ET/EM and/or FIB-SEM for the following project:
http://s.embl.org/HD01243

In case you have further questions, please do not hesitate to contact
me. Closing date for applications is Feb 16.

Best wishes,


Carsten
____________________________________________________________________________________
Dr. Carsten Sachse Group Leader
European Molecular Biology Laboratory Meyerhofstr. 1
69117 Heidelberg
Germany
email: carsten.sachse-at-embl.de
http://www.embl.de/research/units/scb/sachse/
http://www.sachse.embl.de

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From: frank_karl-at-ardl.com
Date: Wed, 7 Feb 2018 13:51:31 -0600
Subject: [Microscopy] Beam always on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,
I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.

Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!


Thanks!!!

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: Duane.Harland-at-agresearch.co.nz
Date: Wed, 7 Feb 2018 14:45:25 -0600
Subject: [Microscopy] Beam always on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank,

I've had a similar issue with our FEI Morgagni 267D (for most purposes similar to the CM12). I'd bet the wehnalt assembly is almost identical.
For us it was almost certainly a filament break in such a way that it was shorting across the wehnalt. I say "almost certainly" because in our case this happened at almost the same time that a bunch of electronics issues occurred and so we had mixed symptoms. But replacing the filament (which we had only just replaced as part of our diagnosis of elimination), did the trick for us.

At least it is a simple check.

Good luck,
Duane

Duane Harland
Senior Scientist
Food & Bio-based Products
T +64 3 321 8710
Based at Lincoln Research Centre Campus agresearch.co.nz
New Zealand

-----Original Message-----
X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com]
Sent: Thursday, 8 February 2018 9:10 AM
To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}

Hello Everyone,
I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.

Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!


Thanks!!!

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: klivi-at-jhu.edu
Date: Wed, 7 Feb 2018 15:31:44 -0600
Subject: [Microscopy] Beam always on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Frank,
This is called Dark Current. It comes from the evaporation of filament material onto the insulator base creating a pathway for electrons to leak to ground, and therefore, creating a current in the filament that causes it to glow with only the kV on. It’s not a problem, but indicates that your filament is old.
Ken

Kenneth JT Livi, PhD
Director, Materials Characterization and Processing Center
Materials Science and Engineering
3400 N Charles Street
Johns Hopkins University
Baltimore, Maryland 21218 USA

} On Feb 7, 2018, at 2:56 PM, frank_karl-at-ardl.com wrote:
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} Hello Everyone,
} I'm using a Philips CM12 and I've been noticing recently I still have a beam and image with the filament supposedly desaturated. I have to turn the high voltage off to get the beam completely gone. I don't remember this scope doing this before. So, I suspect something isn't right but before I request a service call (we are a fugal company) I'd like to confirm that the scope is acting abnormally. I'm also fishing for ideas on what is wrong.
}
} Of course who can't resist wondering where all those people out of the office are and is it warmer than Akron Ohio in February!
}
}
} Thanks!!!
}
} Stay safe...........
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}


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From: jacques.faerber-at-ipcms.unistra.fr
Date: Thu, 8 Feb 2018 10:20:26 -0600
Subject: [Microscopy] OM focus knob motorisation

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Hi all

A collegue in the lab has strong health difficulties with her shoulders
and wrists. She works on a Zeiss Axio Imager D2m with a special stage
and external accessories which leaves only a close access to the
focusing knobs.
So we are looking for a companies which could fit a motorization on the
focus knobs, to have a remote control of them.
Does someone know a (preferably french or europeen) company which could
do this job ? We'll ask Zeiss too, but we have some fear about the price...

Thanks in advance
Jacques

--

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

Tel 00 33(0)3 88 10 71 01
Fax 00 33(0)3 88 10 72 48
E-mail : Jacques.Faerber-at-ipcms.unistra.fr


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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Feb 2018 16:12:55 -0600
Subject: [Microscopy] viaWWW:JEOL 840A SEM video signal

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Email: gary-at-cermetmaterials.com Name: Gary Castelow

Organization: CERMET MATERIALS INC.

Title-Subject: [Filtered] JEOL 840A SEM

Message: Hello all! We randomly lost video signal with our SEM the
other day. Acted as if the filament had blown, but the check gauge is
still reading current when the AC is turned on. Im am by no means an
"electrician" but all connections still look ok going to the viewing
monitor. Is there something fairly easy that goes bad I could check??
Or any other help is greatly appreciated!

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From: microscopy.listserver-at-gmail.com
Date: Fri, 9 Feb 2018 03:08:08 -0600
Subject: [Microscopy] viaWWW:Tungsten filaments

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Email: wschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison
Title-Subject: [Filtered] Tungsten filaments

Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does
anyone have any suggestions for extending filament life? We try and
operate slightly under saturated except for EBSD or EDS mapping. I’ve
heard of “seasoning” the filament but have never seen an actual protocol
or talked with anybody who’s actually done it. Any suggestions would be
appreciated!

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From: diller-at-stefan-diller.com
Date: Fri, 9 Feb 2018 04:19:17 -0600
Subject: [Microscopy] Re: viaWWW:JEOL 840A SEM video signal

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Hello Gary,

did you check your complete video-processing path, like:

- setting the gain of the SE-detector so high to finally see the signal noise (this would state at least a working photomultiplier
/ detector system)

- did you check for the cage voltage switch to be on "positive" ?

- check on HV voltage and cathode heating. Do you see any change by going up in heating?

- centering and e-beam shift potis aligned?

- cathode OK? Current might be a dark currant or mis-functioning high voltage unit

- take out the final aperture and try to find beam...

These are only a few possibilities...


Best wishes,

Stefan



-

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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From: wij.muss-at-aon.at
Date: Fri, 9 Feb 2018 07:10:02 -0600
Subject: [Microscopy] RE: Tungsten filaments [Hitachi S3400N VPSEM, how extending filament life?]

Contents Retrieved from Microscopy Listserver Archives
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Apologize for lengthiness

Bill,
with " } seasoning { a tungsten filament" you perhaps (might) think of slow
and careful -controlled 'heating' of a new filament
(as I have done that with every new filament after mounting in a TEM, a
ZEISS109 with differential pumping system=rotary pump -for RV as well as an
Ion Getter Pump for HV).

IMHO 'slow heating' of the cathode filament before subjecting it to the
"hard" working conditions might be (at least in my experience really 'is' )
of benefit regarding beam stability as well as maintaining your filament's
lifetime.

"Slow heating" means [after change of filament] at least running the new
filament 1-2 hrs 'under-under'-saturated after achievement of an evacuation
optimum of the chamber (not knowing whether you could see anyhow the
filament's image in such a condition like in a TEM on the screen).
You easily would / could see (if you e.g. have permanent vacuum-measurement
monitoring ready) that on starting to "heat"(increase) the filament
(current), the vacuum decreases significantly (at least slightly) because of
"evaporation" of metal & impurities / contaminants.

Then increase filament current a bit more.... and let "equilibrate" vacuum
conditions. You might even find such decreasing vacuum after a third
increasing of the filament heating near the 'saturation' point.

Another helpful requisite also would be to use - without using or even
heating the filament first an ACD (anti-contamination device - if
available) with at least one if not two cycles of cooling first and pumping
the chamber afterwards to get rid of most organic molecules you brought into
the filament chamber by your cleaning and mounting procedures - let warm up
the ACD and pump (RV and HV if possible) again.
According to the specifications of the Hitachi S3400N VPSEM (I found on:
https://www.ntnu.edu/documents/140082/1269041159/S-3400NSpecifications.pdf/6
b94c26f-a9d4-4f36-82b3-7e9eb3603922 ) the possibility of doing so might be
impossible due to the automatic settings of diverse functions but you should
know about those possibilities from practical experience.

Unfortunately a modern VPSEM is not comparable to such an old piece of
equipment like the TEM ZEISS 109 (vintage 1976) I worked with where
maintaining and handling vacuum matters relatively easy...
And, BTW, life time of a tungsten filament always depends also on the
"technical quality" and the handling - when mounting into the chamber -
too....

Best wishes and good luck,
Wolfgang



MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]
A-5020 SALZBURG
sterreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA
Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

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Gesendet: Freitag, 9. Februar 2018 10:29
An: wij.muss-at-aon.at
Betreff: [Microscopy] Tungsten filaments [Hitachi S3400N VPSEM, how
extending filament life?]
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Email: wschneider-at-wisc.edu Name: Bil Schneider
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Title-Subject: Tungsten filaments
Message:

We use tungsten filaments in our Hitachi S3400N VPSEM.
Does anyone have any suggestions for extending filament life? We try and
operate slightly under saturated except for EBSD or EDS mapping.
Ive heard of seasoning the filament but have never seen an actual
protocol or talked with anybody whos actually done it.
Any suggestions would be appreciated!

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From: wesaia-at-iastate.edu
Date: Fri, 9 Feb 2018 12:00:41 -0600
Subject: [Microscopy] viaWWW:Tungsten filaments

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First, what kind of filament life are you currently getting? If you are close to 100 hours, I would be satisfied. If you are only getting 30, then I would look for a problem.

We got better life when we ran at a consistent voltage and didn't have users doing the saturating. We went from average lifetimes of 25 hours to 80 hours. We also operated just under saturation conditions. We simply turned off the high voltage at the end of the session.

We needed to check saturation and reduce current over time. As the filament thinned, it needed less current to reach the same temperature.

Finally, we ran the filament a little further back from the front of the wehnelt. That lessened brightness but increased life.

Warren
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Email: wschneider-at-wisc.edu Name: Bil Schneider
Organization: UW Madison
Title-Subject: [Filtered] Tungsten filaments

Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does
anyone have any suggestions for extending filament life? We try and
operate slightly under saturated except for EBSD or EDS mapping. I've
heard of "seasoning" the filament but have never seen an actual protocol
or talked with anybody who's actually done it. Any suggestions would be
appreciated!

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From: oshel1pe-at-cmich.edu
Date: Fri, 9 Feb 2018 16:58:07 -0600
Subject: [Microscopy] FW: Ask a Microscopist - Imaging board needed for a Leo 1525

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
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Using the "reply" function in your email does *not* send your answer
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Please copy their email address from their question.
***********************************************************************************






Name:karl Hagglund
School:Northwestern University
Grade/Education Level:Graduate
Location:Evanston, IL
US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject}
Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board
Your Question:
Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts.
Thank you,
Karl Hagglund





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From: nmz787-at-gmail.com
Date: Fri, 9 Feb 2018 17:55:35 -0600
Subject: [Microscopy] Re: FW: Ask a Microscopist - Imaging board needed for a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Karl,
If you're in a rush, I would start by contacting these after-market providers:
http://www.jcnabity.com/links.htm#Digital Imaging

If you aren't in a rush, I would find an Electrical Engineering
student who wants a long-term project (lots of EE programs have this
as a graduation requirement) and get them to build one for you (and
ideally require they publish their methods openly). They can start
with this for inspiration and basics:

Electron microscope image capture with an oscilloscope
https://www.youtube.com/watch?v=SWVu-qPR-Ws

Electron microscope image capture via microcontroller (with drill bit animation)
https://www.youtube.com/watch?v=ruuxn2u3yao
http://benkrasnow.blogspot.com/2015/08/drill-bit-in-electron-microscope.html

DIY Scanning Electron Microscope - Sources, Costs and References
http://benkrasnow.blogspot.com/2011/03/diy-scanning-electron-microscope_26.html

SEM data files and image generation script
http://benkrasnow.blogspot.com/2014/11/sem-data-files-and-image-generation.html

DIY Scanning Electron Microscope - Image Quality Improvements
http://benkrasnow.blogspot.com/2011/04/diy-scanning-electron-microscope-image.html
http://benkrasnow.blogspot.com/2011/05/diy-scanning-electron-microscope-image.html
http://benkrasnow.blogspot.com/2011/05/diy-scanning-electron-microscope-image_07.html

Best,
-Nathan

On Fri, Feb 9, 2018 at 3:18 PM, {oshel1pe-at-cmich.edu} wrote:
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} Name:karl Hagglund
} School:Northwestern University
} Grade/Education Level:Graduate
} Location:Evanston, IL
} US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject}
} Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board
} Your Question:
} Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts.
} Thank you,
} Karl Hagglund
}
}
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 00:14:39 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:Tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: James M. Ehrman {jehrman-at-mta.ca}

Bill,

Do you have a high vacuum gauge on the scope? With both our new Hitachi
(SU3500) and our old JEOL (5600), the "ready" state comes on when the
vacuum is in the high 10^-4 torr range (or even higher if there was a
vacuum leak, outgassing sample, or other problem). Running a filament at
such a poor vacuum will drastically reduce filament life and generally
contaminate the column faster. I insist on having a gauge on any scope I
run, and don't turn the beam on until the vacuum is at least mid 10^-5.
With vacuum gauges under $1000, they'll quickly pay for themselves in
terms of filaments and time lost changing them. I've ranted to both JEOL
and Hitachi that they should make them standard on all scopes. By their
own admission, half of their service calls are vacuum related, and with
a gauge the end user can often find a leak and save a service visit. In
our case with the closest service in Ontario, it's a $2000 flight to get
here. Should be a simple budgetary calculation, but Hitachi says I'm the
only customer in Canada who ever requested a vacuum gauge.

I also let the filament cool 5 to 10 minutes whenever possible before
venting. I don't have any hard evidence, but it just seems logical to me
that exposing a hot tungsten filament to atmosphere is going to induce
more oxidation or whatever compared to a cool one.

In my experience, these precautions contribute more to filament life
than slight undersaturation. Of course, you want to make sure you're not
oversaturated, but I get 3-400 hours of the Hitachi filaments running in
the "medium" gun setting.

Hope this helps.

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

Simple, clear purpose and principles give
rise to complex and intelligent behavior.
Complex rules and regulations give rise
to simple and stupid behavior.
— Dee Hock


On 2/9/2018 5:09 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: wschneider-at-wisc.edu Name: Bil Schneider
} Organization: UW Madison
} Title-Subject: [Filtered] Tungsten filaments
}
} Message: We use tungsten filaments in our Hitachi S3400N VPSEM. Does
} anyone have any suggestions for extending filament life? We try and
} operate slightly under saturated except for EBSD or EDS mapping. IÂ’ve
} heard of “seasoning” the filament but have never seen an actual protocol
} or talked with anybody whoÂ’s actually done it. Any suggestions would be
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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 00:31:19 -0600
Subject: [Microscopy] viaWWW:Symposium P06, M&M2018

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Email: okneill-at-umich.edu Name: Owen Neill

Organization: University of Michigan

Title-Subject: [Filtered] Symposium P06, M&M2018

Message: Dear colleagues,

Apologies for the cross-postings. The deadline for abstract submission
for the Microscopy & Microanalysis 2018 Annual Meeting (Baltimore, MD,
5-9 August, 2018) is February 15th, just a week away. We hope you will
consider submitting an abstract to:

Symposium P06, Applications of Integrated Electron Probe Microscopy and
Microanalysis in Characterizing Natural and Synthetic Materials.

A full description of this session is included below – we are looking
for submissions dealing with the synthesis of different microbeam
techniques for evaluating composition, bonding, valence, and
density-of-state in natural and synthetic materials. Papers may be
submitted via the M&M2018 website, which is linked here:
https://www.microscopy.org/MandM/2018/index.cfm

We are also pleased to announce the invited speakers for this symposium:
Emma Bullock, Carnegie Institution
Paul Edwards, Strathclyde University
Raynald Gauvin, McGill University
Peter Statham, Oxford Instruments
Ed Vicenzi, Museum Conservation Institute

We are very excited to hear from these experts in microanalysis, and we
look forward to hearing about your work as well. See you in Baltimore!

The organizers of Symposium P06:
Kat Crispin, Pennsylvania State University
Colin MacRae, CSIRO Mineral Resources
Owen Neill, University of Michigan

Symposium Description:
Electron beam instruments have been merging with other techniques to
extract information such as bonding, valence, and density-of-state,
which, combined with elemental analysis, offers new insights into the
structure and chemistry of materials. Researchers now have access to an
analytical toolbox enabling characterization in a range of controlled
environments (liquid and gas) and conditions (elevated temperatures to
liquid helium). This symposium showcases synergies of new and emerging
techniques, while highlighting recent advances in areas such as optical
analysis using RAMAN, cathodoluminescence, soft X-ray spectrometry, and
others. Papers covering some or all of these new developments and
re-emerging technologies are welcome.

Topics of interest include:
• Multi-technique, electron-microbeam-based characterizations
of natural and synthetic materials.
• Integration of new and existing technologies to measure
material chemistry, structure, boding, valence, etc.
• Applications of such technologies to natural and synthetic
materials.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 00:31:56 -0600
Subject: [Microscopy] viaWWW:

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X-from: mike.marko.em-at-gmail.com

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Email: mike.marko.em-at-gmail.com Name: MIke Marko

Organization: NY

Title-Subject: [Filtered] Opportunity to learn Cryo-EM and get paid for it!

Message: Opportunity to learn Cryo-EM and get paid for it!

Dr. Rajendra Agrawal’s group at Wadsworth Center in Albany, NY is
looking for an electron microscopy technologist for cryo-EM data collection.

This is an opportunity for someone who knows the basics of TEM operation
to learn the “hottest technique in biology”. You will be using high-end
state-of-the-art equipment and software and will be part of an
internationally competitive group that has years of experience and
consistent grant funding. Our group evolved from the pioneering work --
at Wadsworth -- of Joachim Frank, who shared the 2017 Nobel prize.
Other PIs at our 3DEM facility are at the forefront of cryo-EM
development, so Albany can be an exciting place to be. Living expenses
in Albany are moderate, and our area is an attractive place to live.

If you are interested in this opportunity, please contact Dr. Agrawal at
rajendra.agrawal-at-health.ny.gov



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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 18:53:26 -0600
Subject: [Microscopy] viaWWW: nfo on Tungsten Filament optimization ,Message: Thanks to all

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Email: wfschneider-at-wisc.edu Name: Bil Schneider

Organization: UW Madison Geosciences

Title-Subject: [Filtered] Info on Tungsten Filament optimization

Message: Thanks to all who responded to my S3400N VPSEM tungsten
filament questions.

We do try to keep users from venting for 5 minutes after turning off the
HV. We probably average 50 hours per filament lately, which is what
aggravated me a bit because the last box was more like 80 hours. One
challenge is that some users change samples multiple times in a session.
Then the next user is in variable pressure changing mag and kV, vacuum,
etc..... then someone will run an EBSD map overnight at 30 kV, 100 probe
current. We ask a lot from the SEM, in general it’s a workhorse. I did
have several suggestions that seem promising:
The Hitachi guy called and suggested new filaments shouldn’t be turned
on till Vacuum has worked for a couple of hours. He said they try and
wait more like 15 minutes between HV off and venting.
Several responders further emphasized “gently” saturating the filament
over a few hours, keeping it slightly under saturated for general use,
and using some gun bias to concentrate the emission area. One respondent
made a great point about using a vacuum gauge on the gun and waiting
till Vacuum is in the 10 to the minus 5 range. I honestly don’t know
what the vacuum is when the Hitachi lets you turn on the HV.
Adding more spacers to push the grid cap further away from the filament
tip was another suggestion. I was under the impression that how ever
many spacers that each individual filament came with was the correct
distance as measured at the factory to keep emission current appropriate?
Otherwise I handle everything with gloves and care when changing
filaments and occasionally use metal polish on the grid cap and threaded
holder as well as the anode. More often I’ll sonicate the grid cap and
holder in methanol when the gun is open to change a filament. Thanks
again for all the suggestions, I’ll repost this in hopes this collection
of information might be useful to others with tungsten filament questions.
Cheers,
Bil Schneider UW Geosciences

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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Feb 2018 18:54:22 -0600
Subject: [Microscopy] viaWWW:Cross section on MgO substrates

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X-from: corinne.ulhaq-at-ipcms.unistra.fr

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Email: corinne.ulhaq-at-ipcms.unistra.fr Name: Crinne Bouillet

Organization: CNRS

Title-Subject: [Filtered] Cross section on MgO substrates

Message: Hello
I need to study the quality of interface of few nanometer thin layer on
MgO substrate. I 've prepared cross sections by mechanical polishing
followed by ion milling usig the PIPS. I am not satisfied by the quality
of HAADF STEM images, especially when I compared those images to HAADF
STEM images from cross sections on SrTiO3 prepared by the wedge
polishing with the multiprep from ALLIED. Did someone already succeed in
performing cross section on MgO using the wedge polishing? I 've tried
several time without succes. What is the angle used, the strength, the
trick?
Thank you for your help

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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Feb 2018 15:13:49 -0600
Subject: [Microscopy] Fwd: Tungsten filaments

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X-from: Tom Hrn {tomas.hrncir-at-tescan.com}
\
Hello All,
in my experience there are 3 very simple rules to extend the tungsten
filament lifetime:

1. Vent the microscope by nitrogen and always exchange the sample and
pump the chamber quickly. This will minimize the contamination of the
vacuum chamber by water and speed up the pumping.

2. After getting HV ready signal (when the gun/chamber is pumped), wait
several tens of seconds or longer before heating the filament - the
filament lifetime depends on the pressure exponentially. Reaching lower
10-3 Pa in the gun is perfect to extend the filament lifetime.

3. Turn off filament heating manually and wait several minutes before
venting the chamber. Like that, the filament will cool down properly.
Venting the filament when it is hot decreases its lifetime significantly.

By utilizing long waiting time, the filament lifetime around 1000 hours
can be obtained easily, if there is no vacuum leak in the gun. So
sometimes it is a question whether to wait longer and reach very long
lifetime, or wait shorter and exchange filaments more frequently
(changing tungsten filament is cheap and easy). For someone and on some
machines, exchanging the filament might take several minutes only so it
does not make sense to wait several minutes during each sample exchange
procedure. For other users/machines, exchanging the filament might be a
nightmare so it would be better to wait longer and extend the filament
lifetime :-).
Have a nice day,
Tomas

--
Tomas Hrncir, Ph.D.
Senior R&D Physicist

p: +420 530353468
a: TESCAN Brno, s.r.o. | Libuina tda 1, 623 00 Brno, Czech Republic
w: www.tescan.com | e: tomas.hrncir-at-tescan.com


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From: steven.spurgeon-at-pnnl.gov
Date: Mon, 12 Feb 2018 18:08:23 -0600
Subject: [Microscopy] Suggestions for vacuum desiccator sample containers?

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Hello everyone,

We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes.

Does anyone have recommendations for other containers we can consider? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Feb 2018 00:51:59 -0600
Subject: [Microscopy] viaWWW:Electron Microscopist position in Halle, Germany

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X-from: panagiotis.kastritis-at-embl.de

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Email: panagiotis.kastritis-at-embl.de Name: Dr Panagiotis Kastritis

Organization: European Molecular Biology Laboratory, Heidelberg
Title-Subject: [Filtered] Electron Microscopist position in Halle, Germany

Message: Dear EM community,

I would like to draw your attention to an open position of EM facility
manager at the HALOmem institute, located at the Martin Luther
University of Halle-Wittenberg (MLU) in Halle, Germany.
Our cryoEM facility includes the 300 kV JEOL JEM-3200FSC (equipped with
field emission gun, cryo-stage, in-column energy filter and GATAN K2
direct electron detector). Funds are available to acquire another
high-end cryo-electron microscope. We are searching for a staff
scientist/facility manager who will oversee the operation of the cryoEM
facility: assist users with training on the microscopes, perform data
collection and ensure timely maintenance of the microscopes. The
position is offered until August 2022 and the call closes on the 5th of
March. The candidate should have an extensive experience in electron
microscope operation, preferably under cryogenic conditions. The HALOmem
institute (http://www.halomem.de/) is interested in membrane protein
research and houses research groups with strong expertise in protein
biochemistry, X-ray crystallography, NMR and proteomics.
Interested candidates should apply via e-mail or request further
information from Dr. Panagiotis Kastritis (pk-at-halomem.de)
--
Official call (in german):
http://www.verwaltung.uni-halle.de/dezern3/Ausschr/18_138.pdf
English translation:
http://www.halomem.de/images/uploads/EM_manager_ad-eng_inofficial.pdf
Job details (in english):
http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf
(http://www.halomem.de/images/uploads/EM_manager_ad-eng_details.pdf)
---

Thank you very much, Kindest Regards,

Panos
--
Dr. Panagiotis Kastritis
Structural and Computational Biology Unit
European Molecular Biology Laboratory
Meyerhofstra?e 1, 69117 Heidelberg, Germany

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From: lists-at-nexperion.net
Date: Tue, 13 Feb 2018 02:41:03 -0600
Subject: [Microscopy] Re: Suggestions for vacuum desiccator sample containers?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Steven,

} We’re looking for a compact storage solution to keep membrane boxes under vacuum and/or inert atmosphere. I am considering the containers offered by SPI ( https://www.2spi.com/item/01602-ab/spidry-sample-preservers/) and I’m also aware of Ted Pella’s bell jars, which are a bit too large for our purposes.
} Does anyone have recommendations for other containers we can consider? Thanks!

I have been using this model for storage and transport of SEM samples but also whole TEM grid boxes:

http://scienceservices.de/en/sample-stub-vacuum-desiccator.html

They work really well, up to the point where they start questioning you at the airport security check where you'd be going with that "crystal ashtray" ;)

Guenter

--
Dr. Guenter Resch
Nexperion e.U. - Solutions for Electron Microscopy - www.nexperion.net
Ziegelhofstrasse 136/1, 1220 Vienna, Austria - Phone +43 664 94 17 210
Registered at Commercial Court Vienna, FN 397677w - VAT Reg. ATU67962234


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8, 44 -- Subject: Re: [Microscopy] Suggestions for vacuum desiccator sample containers?
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From: diller-at-stefan-diller.com
Date: Tue, 13 Feb 2018 04:18:24 -0600
Subject: [Microscopy] Re: FW: Ask a Microscopist - Imaging board needed for a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Karl,

if your SEM computer has still control of vacuum / high tension and scan amp, the easiest solution would be to attach an external
scan system like the DISS5 from www.pointelectronic.de I am using.

Next possibility would be to exchange the complete electronics to a new one. Pointelectronic does this for some of the Zeiss / FEI
/ Jeol scopes. There should be an US-based agent also for their hardware.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 10.02.18 um 00:21 schrieb oshel1pe-at-cmich.edu:
} ----------------------------------------------------------------------------
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}
} Name:karl Hagglund
} School:Northwestern University
} Grade/Education Level:Graduate
} Location:Evanston, IL
} US Email:karl.hagglund-at-Northwestern.edu {mailto:karl.hagglund-at-Northwestern.eduSubject}
} Subject {mailto:karl.hagglund-at-Northwestern.eduSubject} :Parts Needed Leo/Zeiss 1525 imaging board
} Your Question:
} Our Leo 1525 has suffered a failure on the imaging board resident on its vintage Windows 98 PC. This is a key system on the microscope, and we have learned that Zeiss no longer has replacements available, and there is not a current computer upgrade path. If there a user out there who might have an old PC is the cupboard from their Vintage Zeiss/Leo SEM, I would be very interested in hearing about it. We are looking for replacement boards for our 20 year old LEO PC. These systems have frame grabbing and scan control boards resident on the system computers, and we are in need of replacement parts.
} Thank you,
} Karl Hagglund
}
}
}
}
}
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==============================Original Headers==============================
10, 30 -- From diller-at-stefan-diller.com Tue Feb 13 04:18:23 2018
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Feb 2018 14:07:15 -0600
Subject: [Microscopy] Fwd: Tungsten filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Steve Chapman {protrain-at-emcourses.com}

Hi

I have just picked up this offering and with 50 years EM experience I
fully agree with Tomas' posting. This procedure should be in your
s.o.p. for running a scanning electron microscope, any other way is just
not good enough!

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com



-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
[mailto:microscopy.listserver-at-gmail.com] Sent: 12 February 2018 21:15
To: protrain-at-emcourses.com

X-from: Tom Hrn {tomas.hrncir-at-tescan.com} \ Hello All, in my experience
there are 3 very simple rules to extend the tungsten filament lifetime:

1. Vent the microscope by nitrogen and always exchange the sample and pump
the chamber quickly. This will minimize the contamination of the vacuum
chamber by water and speed up the pumping.

2. After getting HV ready signal (when the gun/chamber is pumped), wait
several tens of seconds or longer before heating the filament - the filament
lifetime depends on the pressure exponentially. Reaching lower
10-3 Pa in the gun is perfect to extend the filament lifetime.

3. Turn off filament heating manually and wait several minutes before
venting the chamber. Like that, the filament will cool down properly.
Venting the filament when it is hot decreases its lifetime significantly.

By utilizing long waiting time, the filament lifetime around 1000 hours can
be obtained easily, if there is no vacuum leak in the gun. So sometimes it
is a question whether to wait longer and reach very long lifetime, or wait
shorter and exchange filaments more frequently (changing tungsten filament
is cheap and easy). For someone and on some machines, exchanging the
filament might take several minutes only so it does not make sense to wait
several minutes during each sample exchange procedure. For other
users/machines, exchanging the filament might be a nightmare so it would be
better to wait longer and extend the filament lifetime :-).
Have a nice day,
Tomas

--
Tomas Hrncir, Ph.D.
Senior R&D Physicist

p: +420 530353468
a: TESCAN Brno, s.r.o. | Libuina tda 1, 623 00 Brno, Czech Republic
w: www.tescan.com | e: tomas.hrncir-at-tescan.com

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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Feb 2018 15:49:37 -0600
Subject: [Microscopy] viaWWW:Career Opportunity - Electron Microscopy Technologist

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Message: Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated
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The individual must have extensive practical expertise in biological
sample preparation for transmission and scanning electron microscopy of
animal tissues and mammalian cell lines. Hands-on knowledge of confocal
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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Feb 2018 03:05:23 -0600
Subject: [Microscopy] viaWWW:GWNIC to host afternoon of high pressure freezing lectures

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] GWNIC to host afternoon of high pressure
freezing lectures

Message: Hi all,

The George Washington University Nanofabrication and Imaging Center
would like to invite you to an afternoon of high pressure freezing
lectures on Monday March 5, 2018. So if you are in Washington DC, just
drop around and spend an inspiring afternoon with some local experts in
the field of high pressure freezing.

https://nic.gwu.edu/high-pressure-freezing

Chris Brantner

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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Feb 2018 03:06:25 -0600
Subject: [Microscopy] viaWWW:old style FEI camera film cassette holder

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Email: tina.williams-at-ars.usda.gov
Name: Tina Williams

Organization: USDA

Title-Subject: [Filtered] old style FEI camera

Message: Hello,

We have an old style FEI camera film cassette holder that fits an FEI
Technai 12. If anyone is interested please contact me offline.

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From: frank_karl-at-ardl.com
Date: Wed, 14 Feb 2018 09:22:45 -0600
Subject: [Microscopy] TEM question answered

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I want to thank everyone who responded to my TEM question and I regret things are so crazy I can't respond to each person. The information provided was very useful and I plan to remove and clean the Wehneit cup and reinstall. That should, I hope resolve my problems.





Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Feb 2018 14:48:17 -0600
Subject: [Microscopy] viaWWW:Job Opening - NASA Johnson Space Center - SEM/EBSD

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Email: Lindsay.P.Keller-at-nasa.gov Name: Lindsay Keller

Organization: NASA

Title-Subject: [Filtered] Job Opening - NASA Johnson Space Center - SEM/EBSD

Message: Job opening at NASA JSC with Jacobs for a Planetary Sample
Scientist specializing in electron beam analyses of materials primarily
using SEM/EBSD techniques. position description and qualifications at:
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From: zaluzec-at-microscopy.com
Date: Wed, 14 Feb 2018 14:50:54 -0600
Subject: [Microscopy] viaWWW:JEM-2100 Plus Parts Need - Virtually Urgent

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X-from: rvancamp-at-kettering.edu

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Email: rvancamp-at-kettering.edu Name: Rick

Organization: Kettering University

Title-Subject: [Filtered] JEM-2100 Plus Parts Need - Virtually Urgent

Message: Hello Everyone,

I am the microscopist at Kettering University. We own a JEM-2100 Plus
TEM. I recently observed that the Wehnhelt and the cathode mounting
base the Wehnhelt screws onto have experienced damage to their mating
threads. This precludes these parts from being used. I am posting this
message to the community in an attempt to identify parts we can obtain
from those that have these two parts in addition to the ability to loan
them to us on a temporary basis or sell them to us.

The JEM-2100 Plus features a Wehnelt and cathode mount that are common
to many Jeol instruments yet, I do not have a complete list. We must
consider consulting Jeol to ensure compatibility before any transaction
occurs. Note this is the Jeol K-type cathode mount. For example, our
TEM uses the Jeol MA113008 (03) tungsten filament, the Denka M3 LKSH
60-degree sharp tip LaB6 cathode, and the APTech mini-Vogel mount 15927
LaB6 cathode. These cathodes are provided as a reference only. I do
not know the complete list of LaB6 cathodes that are compatible with our
JEM-2100 Plus.

Please contact me if you have additional questions or think you are in a
position to help us. Thank you for your consideration.

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From: klappar.panova-at-gmail.com
Date: Wed, 14 Feb 2018 15:25:19 -0600
Subject: [Microscopy] Kikuchi maps

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Hello,
I am teaching a TEM class and I am having trouble finding reliable, readable mappings of the Kikuchi lines for diamond Si. There seems to be a couple of hand-drawn ones floating around, but they are very low-res. I was wondering if anyone had one they could share, or direct me to a place where they can be simulated?

Thank you!

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From: dev.c0debabe-at-gmail.com
Date: Thu, 15 Feb 2018 09:40:41 -0600
Subject: [Microscopy] Norad Quest EDX - system voltage measurements

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Hi,

I recently got a second hand Noran Quest EDX system which I currently
can't power on since the detector temperature is too high.
I let it cool down for 24 hours with a fully filled up dewar.
I believe that the measurement of the "detector temp" is wrong since
there is no voltage (0.00 Volts) measured at all. Also, there is no
voltage difference between a warm detector and a detector that has
cooled down for 24 hours. I am thus looking for technical information
including schematics. I suspect that there could be a contact issue or a
broken cable.

I started the Noran Quest "sys_status" tool. It gives me the following
output:

--------------------------------------------------------------
Detector Temp: 0.00 Volts LN Sensor: 4.28 Volts
Detector Bias: -5.66 Volts Reference: 1.60 Volts
Aux 1: 0.01 Volts Chassis Temp: 6.48 Volts
Aux 2: 0.02 Volts +5V: 4.91 Volts
+24V: 24.06 Volts -24V: -24.05 Volts
+15V: 14.57 Volts -15V: -14.84 Volts
+5V Analog: 4.85 Volts -5V Analog: -5.07 Volts
--------------------------------------------------------------

As you can see, the "detector temp" is 0.00 Volts.


- What are normal voltage levels for the temperature measurements ?
(If the LN sensor voltage is 4.28 Volts, how does that translate
to °C ?
Is there a formula to translate the voltage to temperature?
Is 4.28 Volts OK for a filled up dewar ?)

- What voltage should I get for the "detector temp" ?
(What voltage should be measured if the detector is warm ?
What voltage should be there if the detector is cooled down ?)

- Where is the temperature sensor physically located within the detector ?

- What kind of temperature sensor is it (resistor with X Ohms) ?

- At the detector there is a d-sub connector. What is the pinout of this
connector and where is the "detector temp" output ?


Any technical information to get my detector working would be really
appreciated.

Thank you,
Stefan




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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 03:41:38 -0600
Subject: [Microscopy] viaWWW: ancient grain imaging help needed

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X-from: lisa.odonovan-at-adelaide.edu.au

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Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan

Organization: The University of Adelaide, Adelaide Microscopy

Title-Subject: [Filtered] ancient grain

Message: Hi all,
I have been tasked with imaging an ancient grain. It is 1000 year old
millet and I have one only! I have done SEM/TEM of grain before but not
one so old. I am thinking SEM may be the way to go. The investigators
would like to see the internal structure of the grain (if any) and I
have no idea whether it will be 'normal', caramelised or powder inside!
It must be fixed in order to be released from quarantine so my first
question is should I use an aqueous fixative or alcohol?
Any other advice would be gratefully accepted!

Cheers
Lisa

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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 03:42:46 -0600
Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
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Email: btracy-at-eag.com Name: Bryan Tracy

Organization: EAG Laboratories

Title-Subject: [Filtered] cutting the slab?

Message: Hello Everyone,

I know this is a subject on which reasonable people can disagree, but I
wanted to ask what has been your experience with cutting the slab to
reduce low frequency vibrations?

The floor appears to be moving in the horizontal plane at 1.6Hz (4.4
reduction factor needed) and in the vertical direction at 5HZ. (3.1
reduction factor needed)

much appreciated

bryan

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From: John.Mardinly-at-asu.edu
Date: Fri, 16 Feb 2018 11:24:20 -0600
Subject: [Microscopy] Re: viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Bryan;
3-4X attenuation of ambient vibrations is very difficult. Finding another location would be the preferred solution.

A. John Mardinly, Ph.D., P.E.
The Sleep of Reason Produces Monsters
Francisco Goya



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} Email: btracy-at-eag.com Name: Bryan Tracy
}
} Organization: EAG Laboratories
}
} Title-Subject: [Filtered] cutting the slab?
}
} Message: Hello Everyone,
}
} I know this is a subject on which reasonable people can disagree, but I
} wanted to ask what has been your experience with cutting the slab to
} reduce low frequency vibrations?
}
} The floor appears to be moving in the horizontal plane at 1.6Hz (4.4
} reduction factor needed) and in the vertical direction at 5HZ. (3.1
} reduction factor needed)
}
} much appreciated
}
} bryan
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 14:19:29 -0600
Subject: [Microscopy] viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Lisa,

It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
much anyway. If alcohol is good enough to release from quarantine, use
70 or 80% ethanol, then go through to 100% EtOH.
Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
at 20 deg C.

After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
get a brittle fracture that will expose the starch grains.
This part will be particularly fun (or “fun”) if your grain is tiny ...

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab





Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan

Organization: The University of Adelaide, Adelaide Microscopy

Title-Subject: [Filtered] ancient grain

Message: Hi all,
I have been tasked with imaging an ancient grain. It is 1000 year old
millet and I have one only! I have done SEM/TEM of grain before but not
one so old. I am thinking SEM may be the way to go. The investigators
would like to see the internal structure of the grain (if any) and I
have no idea whether it will be 'normal', caramelised or powder inside!
It must be fixed in order to be released from quarantine so my first
question is should I use an aqueous fixative or alcohol?
Any other advice would be gratefully accepted!

Cheers
Lisa



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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Feb 2018 14:20:40 -0600
Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
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X-from: Mike Toalson {miketoalson-at-gmail.com}


Hi Bryan,

Do you know if the vibration is resulting form sources inside the
building or the from the general area outside?  EAG is in between a
number of freeways there in Sunnyvale and the 101 isn't far away so if
it is the underlying ground below slab, you might make it worse cutting
the slab.  I'm sure some of the experts on here have experience doing
this and will advise.

You might also check with Vibration Engineering Consultants -
www.vibeng.com {http://www.vibeng.com} - in Pacifica, CA. This is their
specialty.

I would also be remiss if I didn't suggest that you consider a Vibration
Isolation Base Platform.  The active type we offer suppresses vibration
starting at 0.7 Hz and has 90% suppression by 2Hz. I would avoid a
passive type isolator as most of them have a resonant frequency right
around 2 to 4 Hz which would make things even worse for the vibrations
you have.  Likewise if you decide to cut the slab, careful if you use an
elastomeric sealant and check it's resonant frequency.

Have a look here at our line of Vibration Isolation Platforms for
SEM/TEM/FIB:
http://www.elementpi.com/active-vibration-isolation-platform-products/

Good Luck,

Mike Toalson
element Pi, LLC
833-314-1593


On Fri, Feb 16, 2018 at 2:15 AM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:





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Email: btracy-at-eag.com {mailto:btracy-at-eag.com} Name: Bryan Tracy

Organization: EAG Laboratories

Title-Subject: [Filtered] cutting the slab?

Message: Hello Everyone,

I know this is a subject on which reasonable people can disagree, but I
wanted to ask what has been your experience with cutting the slab to
reduce low frequency vibrations?

The floor appears to be moving in the horizontal plane at 1.6Hz  (4.4
reduction factor needed) and in the vertical direction at 5HZ.  (3.1
reduction factor needed)

much appreciated

bryan

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From: Rosemary.White-at-csiro.au
Date: Fri, 16 Feb 2018 19:05:22 -0600
Subject: [Microscopy] Re: viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

All,

See below for a request from a paleobiologist here at the National Museum of Natural History. If you have a modern image of collagen fibrils you are willing to share in some manner he would be interested in hearing from you directly. His contact is GreenwaltD-at-si.edu.

Thanks,

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891  mailto:whittaks-at-si.edu
 
SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
https://www.facebook.com/nmnh.fanpage/  |  https://twitter.com/NMNH  |  https://www.instagram.com/smithsoniannmnh/

X-from: "Greenwalt, Dale" {GreenwaltD-at-si.edu}

Hi Lisa,

Fixation in 95-100% methanol or ethanol may be better for your grain - there will be less tissue swelling. The dry grain will contain only about 8-10% water, so going into 100% solvent will be fine. A little water (i.e. 95% solvent) may help penetration. Methanol will penetrate a little better, but it will take some time for any solvent to get deep into a dry grain.

Another option after drying would be high-resolution (+/- phase contrast) x-ray CT - it would quickly show you if the grain had any contents, and give you an idea of what they are without breaking the seed. There are at least a couple of labs I know of in Australia that do this, one of them is here in Canberra at the ANU - https://ctlab.anu.edu.au/, and since your seed will be dead after going through solvent, you don't have to worry about the high kV x-rays killing the tissue. Millet seed is pretty small, so you'd get good resolution of the innards.

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
Canberra, ACT 2601
Australia
Adjunct Prof, EH Graham Centre, CSU
& Research School of Biology, ANU

T 61 2 6246 5475
E rosemary.white-at-csiro.au


On 17/2/18, 7:29 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:




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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Lisa,

It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
much anyway. If alcohol is good enough to release from quarantine, use
70 or 80% ethanol, then go through to 100% EtOH.
Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
at 20 deg C.

After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
get a brittle fracture that will expose the starch grains.
This part will be particularly fun (or “fun”) if your grain is tiny ...

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab





Email: lisa.odonovan-at-adelaide.edu.au Name: Lisa O'Donovan

Organization: The University of Adelaide, Adelaide Microscopy

Title-Subject: [Filtered] ancient grain

Message: Hi all,
I have been tasked with imaging an ancient grain. It is 1000 year old
millet and I have one only! I have done SEM/TEM of grain before but not
one so old. I am thinking SEM may be the way to go. The investigators
would like to see the internal structure of the grain (if any) and I
have no idea whether it will be 'normal', caramelised or powder inside!
It must be fixed in order to be released from quarantine so my first
question is should I use an aqueous fixative or alcohol?
Any other advice would be gratefully accepted!

Cheers
Lisa



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From: microscopy.listserver-at-gmail.com
Date: Sun, 18 Feb 2018 10:52:46 -0600
Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
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X-from: Henk Colijn {colijn.1-at-osu.edu}
Reply-To: Henk Colijn {colijn.1-at-osu.edu}
To: microscopy.listserver-at-gmail.com

Hi Bryan,

Those are very low frequencies. They should be in a range that an active compensation system should
be able to handle it.

Another thing to consider is the resonant frequency of the slab (even though it is damped by the
ground). The more mass in the slab, the lower its resonant frequency. The install guides for our
microscopes show that the microscope is much more sensitive at very low frequencies. The allowable
vibration at 10Hz is approx 10x lower than at 20Hz. Since too much mass can push the slab resonance
down into the range where the microscope is more sensitive you may want to estimate the resonant
frequency of your current slab and then consider whether to slice it or not. It would also be
useful to measure the ground vibration away from the instrument to get an idea of the driving
frequencies.

Good luck!
Henk



--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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To: colijn.1-at-osu.edu
Sent: 2/16/2018 4:45:19 AM


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From: microscopy.listserver-at-gmail.com
Date: Sun, 18 Feb 2018 10:53:17 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}



Thanks Phil.
I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and
then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking
this grain might have a more detrimental effect.
Thanks for your advice

Cheers
Lisa

Dr Lisa O'Donovan

Microscopist

Adelaide Microscopy

Division of Research and Innovation

The University of Adelaide,

ADELAIDE SA 5005

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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:

}
}
}
} ----------------------------------------------------------------------------
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} X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
}
} Lisa,
}
} It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
} much anyway. If alcohol is good enough to release from quarantine, use
} 70 or 80% ethanol, then go through to 100% EtOH.
} Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
} at 20 deg C.
}
} After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
} nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
} get a brittle fracture that will expose the starch grains.
} This part will be particularly fun (or “fun”) if your grain is tiny ...
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
} 989 774-7567 lab
}
}
}
}
}
} Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan
}
} Organization: The University of Adelaide, Adelaide Microscopy
}
} Title-Subject: [Filtered] ancient grain
}
} Message: Hi all,
} I have been tasked with imaging an ancient grain.  It is 1000 year old
} millet and I have one only! I have done SEM/TEM of grain before but not
} one so old.  I am thinking SEM may be the way to go.  The investigators
} would like to see the internal structure of the grain (if any) and I
} have no idea whether it will be 'normal', caramelised or powder inside!
} It must be fixed in order to be released from quarantine so my first
} question is should I use an aqueous fixative or alcohol?
} Any other advice would be gratefully accepted!
}
} Cheers
} Lisa
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Sun, 18 Feb 2018 10:53:45 -0600
Subject: [Microscopy] Fwd: Critical point dryer suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Factor, Jan {Jan.Factor-at-purchase.edu}



Dear List:

I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
faculty and undergraduate student users for research projects, and trains undergraduates via
courses. After training, users carry out their own specimen preps.

Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.

I would appreciate any comments from folks who have experience with either of these, as well as
suggestions about other models I may have overlooked but should consider.

Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
{mailto:jan.factor-at-purchase.edu} .

--Many thanks, *Jan *

Jan Robert Factor, Ph.D.

Professor of Biology

Purchase College, State University of New York

Purchase, NY 10577

Office: 2016 Natural Science Bldg., 914-251-6659

Office Hours (Spring 2018):

Tues., 11:00-12:00, and Thurs., 1:30-2:30

jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}

/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
{http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 08:28:01 -0600
Subject: [Microscopy] Fwd: Re: Fwd: Critical point dryer suggestions

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

We have the Tousimis 815B & 915B. We process a lot of samples of varying sizes (some quite large) &
they do an excellent job & are easy/straightforward to use. We have an advantage since we're pretty
much next door to the company, but they are very helpful. I haven't used the EMS.

Deborah

Sent from my iPhone

} On Feb 18, 2018, at 11:16 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
}
}
}
}
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} X-from: Factor, Jan {Jan.Factor-at-purchase.edu}
}
}
}
} Dear List:
}
} I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
} faculty and undergraduate student users for research projects, and trains undergraduates via
} courses. After training, users carry out their own specimen preps.
}
} Two models Ive come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
}
} I would appreciate any comments from folks who have experience with either of these, as well as
} suggestions about other models I may have overlooked but should consider.
}
} Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
} {mailto:jan.factor-at-purchase.edu} .
}
} --Many thanks, *Jan *
}
} Jan Robert Factor, Ph.D.
}
} Professor of Biology
}
} Purchase College, State University of New York
}
} Purchase, NY 10577
}
} Office: 2016 Natural Science Bldg., 914-251-6659
}
} Office Hours (Spring 2018):
}
} Tues., 11:00-12:00, and Thurs., 1:30-2:30
}
} jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}
}
} /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
} {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
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From: microscopy.listserver-at-gmail.com
Date: Sunday, 18February, 2018 at 12:29 To: Philip Oshel
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Lisa,

Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve
used it for corn and barley, but if you do have millet … oof.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








-----Original Message-----
X-from: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}
Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}

X-from: Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au}



Thanks Phil.
I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and
then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking
this grain might have a more detrimental effect.
Thanks for your advice

Cheers
Lisa

Dr Lisa O'Donovan

Microscopist

Adelaide Microscopy

Division of Research and Innovation

The University of Adelaide,

ADELAIDE SA 5005

T: +61 8 8313 4074|

E: Lisa.ODonovan-at-adelaide.edu.au {mailto:Lisa.ODonovan-at-adelaide.edu.au}

Visit our website athttp://www.adelaide.edu.au/microscopy/

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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} " {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:

}
}
}
} ----------------------------------------------------------------------------
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} X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
}
} Lisa,
}
} It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
} much anyway. If alcohol is good enough to release from quarantine, use
} 70 or 80% ethanol, then go through to 100% EtOH.
} Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
} at 20 deg C.
}
} After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
} nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
} get a brittle fracture that will expose the starch grains.
} This part will be particularly fun (or “fun”) if your grain is tiny ...
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
} 989 774-7567 lab
}
}
}
}
}
} Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} Name: Lisa O'Donovan
}
} Organization: The University of Adelaide, Adelaide Microscopy
}
} Title-Subject: [Filtered] ancient grain
}
} Message: Hi all,
} I have been tasked with imaging an ancient grain. It is 1000 year old
} millet and I have one only! I have done SEM/TEM of grain before but not
} one so old. I am thinking SEM may be the way to go. The investigators
} would like to see the internal structure of the grain (if any) and I
} have no idea whether it will be 'normal', caramelised or powder inside!
} It must be fixed in order to be released from quarantine so my first
} question is should I use an aqueous fixative or alcohol?
} Any other advice would be gratefully accepted!
}
} Cheers
} Lisa
}
}
}
} ==============================Original Headers==============================
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From: gilpin-at-purdue.edu
Date: Mon, 19 Feb 2018 16:15:22 -0600
Subject: [Microscopy] evaporating Tin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Jan,

Tousimis is good, I’ve used those a lot. Not the Autosamdri, but the previous model. Have you
considered the Polaron “bomb”? The larger sample chamber (in the regular size) is very handy. SPI
sells this as a rebranded product. I use it in a multi-user facility that teaches undergrads and
grad students.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








-----Original Message-----
X-from: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}
Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}

X-from: Factor, Jan {Jan.Factor-at-purchase.edu}



Dear List:

I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
faculty and undergraduate student users for research projects, and trains undergraduates via
courses. After training, users carry out their own specimen preps.

Two models I’ve come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.

I would appreciate any comments from folks who have experience with either of these, as well as
suggestions about other models I may have overlooked but should consider.

Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
{mailto:jan.factor-at-purchase.edu} .

--Many thanks, *Jan *

Jan Robert Factor, Ph.D.

Professor of Biology

Purchase College, State University of New York

Purchase, NY 10577

Office: 2016 Natural Science Bldg., 914-251-6659

Office Hours (Spring 2018):

Tues., 11:00-12:00, and Thurs., 1:30-2:30

jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}

/_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
{http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
*Please consider the environment before printing this e-mail*

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From trojlita384otoj-at-gmail.com Mon Feb 19 10:46:40 2018
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Hi Everyone,
I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.

We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Campus-wide Coordinator for Electron Microscopy
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx





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From: colijn.1-at-osu.edu
Date: Mon, 19 Feb 2018 17:10:42 -0600
Subject: [Microscopy] evaporating Tin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chris,

As the evaporated films get thicker, internal stress becomes
significant. I'm not sure how bad tin films are but I've had issues
with the films peeling when they got much over 0.1um thickness.

Good luck,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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From: wesaia-at-iastate.edu
Date: Mon, 19 Feb 2018 20:25:11 -0600
Subject: [Microscopy] evaporating Tin

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Considering that is more than 100 times the thickness you normally put down, that is an issue.

I tried using evaporation to put down a thick layer of antimony on glass before. First, when I calculated the mass of Sb to put down a micron at a certain distance, I found I had to fill my tungsten basket to the full and maybe even run a couple of times. Then I think I found the thermal stresses Henk described. The layer curled up off the glass.

Why do they want such a thickness?

Warren

-----Original Message-----
X-from: gilpin-at-purdue.edu [mailto:gilpin-at-purdue.edu]
Sent: Monday, February 19, 2018 4:17 PM
To: Straszheim, Warren E [BIOTC]

Hi Everyone,
I have been asked if our vacuum evaporator will put down tin to 1 micron thickness.

We have a Cressington unit so its not the principle it’s the practice. Does anyone have experience with tin or a thickness of 1 micron or both!

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility Campus-wide Coordinator for Electron Microscopy Purdue University Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx





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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:54:05 -0600
Subject: [Microscopy] viaWWW:GWNIC EM position posted

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X-from: chrisbrantner-at-gwu.edu


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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: The George Washington University

Title-Subject: [Filtered] GWNIC EM position posted

Message: Greetings fellow microscopists,

I want to draw your attention to a position that I have open in my lab. If you are interested,
please follow the links.

https://www.gwu.jobs/postings/49625

https://www.gwu.jobs/postings/49512

Chris Brantner
Senior Research Scientist, Electron Microscopy
George Washington University Nanofabrication and Imaging Center



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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:55:27 -0600
Subject: [Microscopy] Fwd: Re: Critical point dryer suggestions

Contents Retrieved from Microscopy Listserver Archives
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X-from: Matt Russell {Matt.Russell-at-crick.ac.uk}



Hi Jan,

We have a CPD300 here that users have access to. One of our users is only trained on our bench top
SEM and she uses it regularly, independently.

Top tips for things that might catch out students;

1. As per the manual, the fillers should be on top of the holders. If you have them the other way
round the chamber doesn’t fill up correctly
2. Don’t overfill the chamber. Should be OK as long as the level is below the outlet hole at the
top, otherwise you might have some liquid left at the end.

It’s easy to use with a simple user interface; it’s been easy to train people on it. We use it quite
a lot and it gives nice results.

Cheers,

Matt

Matt Russell, PhD
Senior Laboratory Research Scientist
Electron Microscopy STP
The Francis Crick Institute
1 Midland Road
London
NW1 1AT

*T:* +442037962006
*E:* matt.russell-at-crick.ac.uk {mailto:matt.russell-at-crick.ac.uk}
*W:* www.crick.ac.uk {http://www.crick.ac.uk}

} On 18 Feb 2018, at 17:36, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
} wrote:
}
}
}
}
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} X-from: Factor, Jan {Jan.Factor-at-purchase.edu {mailto:Jan.Factor-at-purchase.edu} }
}
}
}
} Dear List:
}
} I am considering replacing an older critical point dryer for a multi-user microscopy lab that serves
} faculty and undergraduate student users for research projects, and trains undergraduates via
} courses. After training, users carry out their own specimen preps.
}
} Two models I抳e come across are the Leica EMCPD300 and the Tousimis Autosamdri-931.
}
} I would appreciate any comments from folks who have experience with either of these, as well as
} suggestions about other models I may have overlooked but should consider.
}
} Feel free to respond directly to me if you would like at jan.factor-at-purchase.edu
} {mailto:jan.factor-at-purchase.edu}
} {mailto:jan.factor-at-purchase.edu} .
}
} --Many thanks, *Jan *
}
} Jan Robert Factor, Ph.D.
}
} Professor of Biology
}
} Purchase College, State University of New York
}
} Purchase, NY 10577
}
} Office: 2016 Natural Science Bldg., 914-251-6659
}
} Office Hours (Spring 2018):
}
}  牋牋燭ues., 11:00-12:00, and Thurs., 1:30-2:30
}
} jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu} {mailto:jan.factor-at-purchase.edu}
}
} /_*Purchase College ranked in the Top 10 public liberal arts colleges nationally*
} {http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public} _/
} *Please consider the environment before printing this e-mail*
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:56:47 -0600
Subject: [Microscopy] Fwd: Re: Fwd: viaWWW: Vibrations: cutting the slab?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Torraca, Gianni {gtorraca-at-amgen.com}

Cutting the slab is dangerous can may compromise the building. It is better to install active
vibration isolation. I have a number of SEMs in Thousand Oaks, CA. One LaB6 system is on the 2nd
floor. We use Herzan to reach vendor spec resolution with gold on carbon.
Regards
Gianni
________________________________________________________________________________________________________________________________
Gianpiero Torraca | Sr. Scientist | ATO Forensics | One Amgen Center Drive | Thousand Oaks, CA 91320
| Mailstop B30E-2B | 805.447.7445
“Mistakes are a fact of life. It is the response to the error that counts.” -Nikki Giovanni




-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday,
February 16, 2018 12:46 PM
To: Torraca, Gianni {gtorraca-at-amgen.com}

X-from: Darrell Miles {milesd-at-us.ibm.com}



One thing I don't think has been mentioned, is that the soil under many slab floors has settled, and
the slab is "floating" above without being supported. I have heard of holes being drilled and a
foam material being injected under the slab to support and dampen vibrations. This is done
sometimes, in addition to other vibration isolation measures.

Regards,
Darrell



microscopy.listserver-at-gmail.com wrote on 02/18/2018 11:56:14 AM:

} From: microscopy.listserver-at-gmail.com
} To: milesd-at-us.ibm.com
} Date: 02/18/2018 10:52 AM
} Subject: [Microscopy] Fwd: viaWWW: Vibrations: cutting the slab?
}
}
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} X-from: Henk Colijn {colijn.1-at-osu.edu}
} Reply-To: Henk Colijn {colijn.1-at-osu.edu}
} To: microscopy.listserver-at-gmail.com
}
} Hi Bryan,
}
} Those are very low frequencies. They should be in a range that an
} active compensation system should
} be able to handle it.
}
} Another thing to consider is the resonant frequency of the slab
} (even though it is damped by the
} ground). The more mass in the slab, the lower its resonant
} frequency. The install guides for our
} microscopes show that the microscope is much more sensitive at very
} low frequencies. The allowable
} vibration at 10Hz is approx 10x lower than at 20Hz. Since too much
} mass can push the slab resonance
} down into the range where the microscope is more sensitive you may
} want to estimate the resonant
} frequency of your current slab and then consider whether to slice it
} or not. It would also be
} useful to measure the ground vibration away from the instrument to
} get an idea of the driving
} frequencies.
}
} Good luck!
} Henk
}
}
}
} --------------------
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
}
} colijn.1-at-osu.edu 614/643-3458
} cemas.osu.edu
}
} "Time is that quality of nature which keeps things from happening
} all at once." (Ray Cummings - 1922)
} Lately it doesn't seem to be working.
}
} ------ Original Message ------
} X-from: microscopy.listserver-at-gmail.com
} To: colijn.1-at-osu.edu
} Sent: 2/16/2018 4:45:19 AM
} Subject: [Microscopy] viaWWW: Vibrations: cutting the slab?
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} } Email: btracy-at-eag.com Name: Bryan Tracy
} }
} } Organization: EAG Laboratories
} }
} } Title-Subject: [Filtered] cutting the slab?
} }
} } Message: Hello Everyone,
} }
} } I know this is a subject on which reasonable people can disagree, but I
} } wanted to ask what has been your experience with cutting the slab to
} } reduce low frequency vibrations?
} }
} } The floor appears to be moving in the horizontal plane at 1.6Hz (4.4
} } reduction factor needed) and in the vertical direction at 5HZ. (3.1
} } reduction factor needed)
} }
} } much appreciated
} }
} } bryan
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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Feb 2018 20:57:28 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help

Contents Retrieved from Microscopy Listserver Archives
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X-from: LNMA CMN Siglo XXI {lnma.cmn.sigloxxi-at-gmail.com}



Hi, Lisa.
I am not an expert, but I would choose an analysis method knowing whether I will need to keep the
seed, or it can be used entirely.
Maybe I would try splitting it in parts (yep, it's millet, but still..) and then try different
several fixation methods.
I would also probably opt for hydration of the seed before fixation, but on an EtOH — to prevent
germination[?] or fungal growth vapor bath.
Interesting task!

Cheers,
Vad

On Mon, Feb 19, 2018 at 9:03 AM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
To: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} }

Lisa,

Good luck. I like Rosemary’s suggestion — mine about liquid nitrogen needs decent sized seeds. I’ve
used it for corn and barley, but if you do have millet … oof.

Phil
------------- Philip Oshel     Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab








-----Original Message-----
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Date: Sunday,  18February, 2018 at 12:29 To: Philip Oshel {oshel1pe-at-cmich.edu
{mailto:oshel1pe-at-cmich.edu} }
Subject: [Microscopy] Fwd: Re: viaWWW: ancient grain imaging help needed




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X-from:         Lisa Anne O'Donovan {lisa.odonovan-at-adelaide.edu.au
{mailto:lisa.odonovan-at-adelaide.edu.au} }



Thanks Phil.
I’ve imaged grain before and I’ve found the best result after soaking it for 48 hrs in water and
then cutting it into pieces before fixing and dehydration. Unfortunately I have no idea if soaking
this grain might have a more detrimental effect.
Thanks for your advice

Cheers
Lisa

Dr Lisa O'Donovan

Microscopist

Adelaide Microscopy

Division of Research and Innovation

The University of Adelaide,

ADELAIDE SA 5005

T: +61 8 8313 4074|

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On 17 Feb 2018, at 6:14 am, "microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com}
{mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } "
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
{mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } wrote:

}
}
}
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} X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu}
{mailto:oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } }
}
} Lisa,
}
} It’s a grain, so largely starch, meaning formaldehyde and glut won’t fix
} much anyway. If alcohol is good enough to release from quarantine, use
} 70 or 80% ethanol, then go through to 100% EtOH.
} Either dry from ethanol or go to tert-butyl alcohol and vacuum sublimate
} at 20 deg C.
}
} After the 2nd or 3rd 100% EtOH, you could put the grain in liquid
} nitrogen and hit it with a razor blade, maybe gently crush it. You’ll
} get a brittle fracture that will expose the starch grains.
} This part will be particularly fun (or “fun”) if your grain is tiny ...
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
} 989 774-7567 lab
}
}
}
}
}
} Email: lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au}
{mailto:lisa.odonovan-at-adelaide.edu.au {mailto:lisa.odonovan-at-adelaide.edu.au} } Name: Lisa O'Donovan
}
} Organization: The University of Adelaide, Adelaide Microscopy
}
} Title-Subject: [Filtered] ancient grain
}
} Message: Hi all,
} I have been tasked with imaging an ancient grain.  It is 1000 year old
} millet and I have one only! I have done SEM/TEM of grain before but not
} one so old.  I am thinking SEM may be the way to go.  The investigators
} would like to see the internal structure of the grain (if any) and I
} have no idea whether it will be 'normal', caramelised or powder inside!
} It must be fixed in order to be released from quarantine so my first
} question is should I use an aqueous fixative or alcohol?
} Any other advice would be gratefully accepted!
}
} Cheers
} Lisa
}
}
}
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--

Laboratorio Nacional de Microscopía Avanzada
Centro Médico Nacional Siglo XXI
Instituto Mexicano del Seguro Social
Av. Cuauhtémoc, No. 330, Col. Doctores
Mexico DF, México, 06720
Ubicación: https://goo.gl/3dsP0b
Tel: +52 (55) 5627 6900 * 20926, 20925
facebook.com/LMNA.CMN.SigloXXI {http://facebook.com/LMNA.CMN.SigloXXI}

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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Feb 2018 08:52:54 -0600
Subject: [Microscopy] viaWWW:BIO TEM workshop

Contents Retrieved from Microscopy Listserver Archives
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Email: jpshield-at-uga.edu Name: John P Shields

Organization: University of Georgia

Title-Subject: [Filtered] Two seats left! - BIO TEM workshop

Message: Biological TEM Workshop

This intensive, three-day workshop provides a practical and basic theoretical introduction to the
Transmission Electron Microscope and biological sample preparation techniques. Each day consists of
lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Monday through Wednesday, March 14-16, 2018, 8am-5pm each day (lunch is provided)

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: March 5, 2018


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From: nathaniel.rieders-at-montana.edu
Date: Fri, 23 Feb 2018 13:37:09 -0600
Subject: [Microscopy] SEM Resolution Standard using FRC Method

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Hello, I am wondering if anybody can reccomend a standard/sample for determining microscope resolution in a SEM using the Fourier Ring Correlation (Autocorrelation) method. It is my understanding that suitable samples for TEM resolution estimates using FRC will not work in an SEM due to the differences in contrast mechanisms. Ideally, these would be samples which exhibit spatial frequencies from DC up to and beyond the resolution limit of the microscope.

Thank You,
Nathaniel Rieders

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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Feb 2018 18:59:27 -0600
Subject: [Microscopy] viaWWW: Microscopy Position Tescan USA

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Email: rabara-at-tescan-usa.com Name: Michal Rabara

Organization: TESCAN USA, Inc.

Title-Subject: [Filtered] Microscopy Position Posted

Message: Organization: Tescan USA, Inc.

Email response: rabara-at-tescan-usa.com
Michal Rabara, CEO & President Tescan USA, Inc.

I would like to draw your attention to the Applications Manager position that we have open in North
America for Tescan USA, Inc. If you are interested, please follow the link below:

https://www.linkedin.com/jobs/view/602245101/


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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Feb 2018 18:48:16 -0600
Subject: [Microscopy] viaWWW:Zeiss Libra

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Email: jcaughey-at-su-group.com Name: Jim Caughey

Title-Subject: [Filtered] Zeiss Libra

Message: Does anyone know of a qualified ISO that can support this TEM - Zeiss Libra 120 Plus TEM?

Thanks for any help!

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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Feb 2018 18:49:22 -0600
Subject: [Microscopy] viaWWW:Applications Scientist TEM Semiconductors at Thermo Fisher

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Email: sorin.lazar-at-fei.com Name: Sorin Lazar

Organization: Thermo Fisher Scientific

Title-Subject: [Filtered] Applications Scientist TEM Semiconductors at Thermo Fisher Scientific,
Eindhoven

Message: Dear TEM community,

I would like to draw your attention to an Applications Scientist TEM Semiconductors open position at
Thermo Fisher Scientific in the Eindhoven Nanoport.

For those interested please follow the link below:

http://jobs.thermofisher.com/ShowJob/Id/156548/Applications-Scientist-TEM-Semiconductors/


Thanks and regards,

Sorin Lazar



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From: microscopy.listserver-at-gmail.com
Date: Tue, 27 Feb 2018 08:53:04 -0600
Subject: [Microscopy] viaWWW:Xe Plasma FIB consumables?

Contents Retrieved from Microscopy Listserver Archives
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Email: leonarddn-at-ornl.gov Name: Donovan Leonard

Organization: ORNL

Title-Subject: [Filtered] Xe Plasma FIB consumables?

Message: Dear Xe Plasma FIB/SEM owners,

I am interested in learning what the potential cost of ownership of a Xe PFIB can be. In addition
to the service contract, what costs associated with apertures and sources are there? I am assuming
apertures have to be changed more frequently since beam currents can be on the order of microamps
(depending on hours in use). What about the Xe gas? I think this is just a small lecture bottle of
gas, but how often do you have to replenish that source?

Please respond off list to leonarddn-at-ornl.gov and thank you in advance. If you want to include any
anecdotes about Xe PFIB ownership I am happy to learn about that too (e.g. nightmares or winning
scenarios).

Donovan

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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Feb 2018 07:56:07 -0600
Subject: [Microscopy] viaWWW:Job Opening Applications Scientist TEM Materials Science at

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: sorin.lazar-at-fei.com

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Email: sorin.lazar-at-fei.com Name: Sorin Lazar

Organization: Thermo Fisher Scientific

Title-Subject: [Filtered] job opening

Message:

"Dear TEM community,

I would like to draw your attention to an Applications Scientist TEM Materials Science open position
at Thermo Fisher Scientific in the Eindhoven Nanoport.

For those interested please follow the link below:
http://jobs.thermofisher.com/ShowJob/Id/156547/Applications-Scientist-TEM-Materials-Science/

Thanks and regards,
Sorin Lazar
"

With many thanks in advance,
Sorin





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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Feb 2018 13:34:42 -0600
Subject: [Microscopy] Fwd: vacuum system on a FEI Morgagni TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Pedro Leão {pedroleao-at-micro.ufrj.br}



Hi group.

I just change the filament on a FEI Morgagni TEM.
As I put the filament on the microscope and close the collum the PVP
does not start.

I reset the system, open the column again, I have no idea of what to do
next. Have anyone already had this problem? Any tips?

Thank you for your attention.

Best,

Pedro Leão

--
---

Instituto de Microbiologia - UFRJ
Laboratório de Biologia Celular e Magnetotaxia
CCS - Centro de Ciências da Saúde - Bloco I
Avenida Carlos Chagas Filho, 373
Cidade Universitária
CEP. 21941-902
Rio de Janeiro - RJ - Brasil
CV Lattes: http://lattes.cnpq.br/9781167318809464
LinkedIn:https://br.linkedin.com/in/pedro-leão-55992853
{https://br.linkedin.com/in/pedro-le%C3%A3o-55992853}
Tel. +55 21 3938 6738
Cel. +55 21 98131 3360




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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Feb 2018 19:44:15 -0600
Subject: [Microscopy] viaWWW:ORNL Seeks Postdoc, Helium Ion Microscopy

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X-from: millerme-at-ornl.gov

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Postdoc, Helium Ion Microscopy

Message: Postdoctoral Research Associate in Helium Ion Microscopy

Oak Ridge National Laboratory (ORNL) is looking for a Postdoctoral Research Associate to conduct
Helium Ion Microscopy (HIM) research and Secondary Ion Mass Spectrometry (SIMS) of a broad range of
inorganic and soft materials. Specifically, your research in this role will focus on imaging of soft
materials. For this role, experience in mass spectrometry (MS) and helium ion microscopy (HIM)
techniques, and/or other closely related areas is ideal. You will work with scientists and users at
the CNMS in developing new HIM capabilities in chemical imaging using SIMS, multimodal data
analysis, and integration with high performance computing environments. You will benefit from
experience in the analysis and characterization of soft materials and inorganic materials.

For more details, including the qualifications for this position, visit: http://bit.ly/PostdocHIM

ORNL is an equal opportunity employer. All qualified applicants, including individuals with
disabilities and protected veterans, are encouraged to apply.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Mar 2018 08:47:54 -0600
Subject: [Microscopy] Fwd: Save the date! North Atlantic Microscopy Society (NAMS) Nov. 1,

Contents Retrieved from Microscopy Listserver Archives
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X-from: Gary Laevsky {glaevsky.lists-at-gmail.com}


Hi All!

We are very proud and excited to announce the formation of a new society, The North Atlantic
Microscopy Society (NAMS). Geographically centered at Princeton, NJ, we envision our coverage to
span southern New York, New Jersey and into Pennsylvania.


Edwin Hubble famously said, “Equipped with his five senses, man explores the universe around him and
calls the adventure Science.” We believe that some of this exploratory instinct has been muted
lately by our disciplinary silos. Individually, we have become exceptional in our specialties and do
not take moments to appreciate the many discoveries happening across the entire spectrum of science.

Our mission is to bridge these silos through the lens of microscopy. We seek to achieve this mission
by promoting microscopy education, stimulating networking among microscopists, and
disseminating microscopy knowledge and skills to the public in the region.


Our first Symposium will be held at Princeton University on November 1, 2018.

Our first speakers will be;

Hari Shroff (Light) - Chief of NIBIB's Section on High Resolution Optical Imaging laboratory.
Nieng Yan (Cryo-EM) - Shilrley M. Tilghman Professor of Molecular Biology at Princeton University.


This is exciting!!!

Gary Laevsky (Light Imaging)
Paul Shao (EM Imaging)
Tharan Srikumar (Mass Spec Imaging)


--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

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From: ALawrence-at-i2at.msstate.edu
Date: Fri, 2 Mar 2018 07:52:41 -0600
Subject: [Microscopy] Student help at the M&M 2018 meetings

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Greetings
The deadline for paper submission has passed and meeting registration is now open. As you are making plans to attend the Baltimore, MD meetings (Aug. 5-9) please don't forget about MSA's student bursary program. Its purpose is to encourage students to attend the meetings by helping to defray some of the costs while giving them an opportunity to meet and interact with the established microscopy community.

The student bursaries will be paid $10 an hour to work for ~20 hours during the meeting or pre-meeting events (paid by check at the end of the meetings). The jobs involve such things as providing support in the different symposia, staffing the volunteer office, newsletter distribution, and helping with vendor tutorial sign-up or in the outreach booth.

Once the program has been finalized, each registered bursary will be contacted and given the chance to choose the times and activities they would like to help with. There is an additional bonus of $10 cash for each morning and/or afternoon session worked to assist with meals, along with a meeting t-shirt.

If anyone would like to participate in the bursary program, please check the box "I wish to apply for a student bursary" on the registration form. Applicants for the bursaries must be a member of MSA or MAS and enrolled as a student at a recognized educational institution. Participants are responsible for their own registration fee and travel expenses.

For those of you who may not be students, but would still like to assist with the meetings, volunteers are also needed to fill the above-mentioned jobs. Although not paid the $10/hour as the students, volunteers do get a meeting t-shirt and the $10 cash for each morning/afternoon worked to help with meals.

If anyone has any questions about the bursary/volunteer program, or would like to participate, contact Amanda Lawrence: mailto:aml1819454-at-gmail.com (please do not reply to the email associated with this post). Bursary space is limited, so sign-up early.

See everyone in Baltimore,

Amanda Lawrence
Student Bursary/Volunteer Coordinator
Microscopy Society of America
mailto:aml181954-at-gmail.com



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From: vray-at-partbeamsystech.com
Date: Fri, 2 Mar 2018 15:30:00 -0600
Subject: [Microscopy] PSF extraction and convolution/deconvolution software or plug-in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I am hoping someone could recommend somewhat-reliable software or (open source plug-in preferred) for extracting (approximated) 2D PSF from a pair of images of the same object, taken with closely matched contrast and same digital pixel count, but different beam current/diameter/profile. One of the images would be significantly sharper and serve as a reference, while the other "blurry" one is assumed to be a convolution of the reference image and PSF of the beam/instrument. The interest would be in extracting and examining PSF from the pair of images, convoluting extracted PSF with sharp image to estimate blurred one, and de-convoluting PSF from the blurred image to reconstruct sharp version. I am finding multiple plugins, but don't seem to come across something that would be convenient for all three operations.

Thank you very much beforehand,

Valery Ray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

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From: steven.spurgeon-at-pnnl.gov
Date: Fri, 2 Mar 2018 17:48:49 -0600
Subject: [Microscopy] Opening for SEM technologist and technician at PNNL

Contents Retrieved from Microscopy Listserver Archives
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Hello everyone,

I wanted to let you know that we’ve got two openings here at PNNL for an SEM technician and technologist to support imaging and sample preparation of nuclear materials. Candidates with a background in materials science and electron microscopy would be ideal.

Please feel free to forward the following links to anyone you think might be interested and don’t hesitate to contact me with any questions. Thank you.

https://pnnl.jobs/richland-wa/senior-technologist-nuclear-chemistry-engineering/2574E37174FD4D788A76BB296C193A15/job/

https://pnnl.jobs/richland-wa/senior-technician-nuclear-chemistry-engineering/1E805E40F8F245CDAD57E49E1B4D0DD7/job/

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com {http://www.stevenspurgeon.com/}
www.pnnl.gov {http://www.pnnl.gov/}



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9, 37 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov}
9, 37 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
9, 37 -- Subject: Opening for SEM technologist and technician at PNNL
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From: microscopy.listserver-at-gmail.com
Date: Sat, 3 Mar 2018 09:54:33 -0600
Subject: [Microscopy] viaWWW: ORNL Seeks Technical Associate Staff Member - Scanning Probe

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Technical Associate Staff Member - Scanning Probe Microscopy

Message: Technical Associate Staff Member - Scanning Probe Microscopy

Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff Member to support scanning
probe microscopy efforts at the Center for Nanophase Materials Sciences (CNMS) at Oak Ridge National
Laboratory (ORNL). In this role, you will be part of the Scanning Probe Microscopy Group, which is a
leader in the development and application of scanning probe microscopies and spectroscopies to image
and manipulate materials functionality in complex materials, ionic and electronic conductors,
molecular assemblies, and nanoscale structures. A suite of 18 commercial and internally developed
atomic force microscopes in ambient and controlled environments and scanning tunneling microscopes
in ultrahigh vacuum systems are used for internal and user science, with emerging opportunities in
enhanced data analytics and multimodal/chemical imaging.

For more details, including the qualifications for this position, visit: http://bit.ly/TP-SPM
ORNL is an equal opportunity employer. All qualified applicants, including individuals with
disabilities and protected veterans, are encouraged to apply.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 5 Mar 2018 08:55:38 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW:PhD_scholarship_in_In-situ_TEM_and_Applications_of?=

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Email: kristian.molhave-at-nanotech.dtu.dk Name: Kristian Molhave

Organization: DTU Nanotech

Title-Subject: [Filtered] PhD scholarship in In-situ TEM and Applications of Nanowire Vapor Phase
Epitaxy Microsystems (VPE)

Message: We have a new PhD position open and I hope you will announce it on your website to help
recruiting!

PhD scholarship in In situ TEM and Applications of Nanowire Vapor Phase Epitaxy Microsystems (VPE)

please apply online via
http://www.nanotech.dtu.dk/about-ntch/ledige-stillinger/job?id=fd61bf71-1ffa-4e21-9a8c-d01c4c5f19a7

In this PhD project you will develop, fabricate and use microfabricated heater systems to study the
processes of III-V nanowire growth with MOVPE in-situ TEM, thereby creating nanowire devices while
imaging the process live!

Contact information:
- Kristian Mlhave, DTU Nanotech, e-mail: kristian.molhave-at-nanotech.dtu.dk
- Kimberly Dick Thelander, Solid State Physics, Lund University, e-mail:
kimberly.dick_thelander-at-ftf.lth.se
- Nika Akopian, DTU Photonics, e-mail: nikaak-at-fotonik.dtu.dk

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From: microscopy.listserver-at-gmail.com
Date: Mon, 5 Mar 2018 19:49:12 -0600
Subject: [Microscopy] viaWWW:thermal treatment of carbon-formvar film

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Email: dlowry-at-asu.edu Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] thermal treatment of carbon-formvar film

Message: a researcher here wants to do some thermal treatments (up to 150C) of samples after they
have been adhered to carbon-formvar coated silicon wafers (approx 13mm x 36mm) containing small
holes, and inquired with me about the max temp these films can endure without failing. She has
tested a blank film at her max temp and the film appeared to remain intact during heating, but fell
apart when removed from oven and during cool down. We have searched for published info on thermal
resistance of carbon-formvar but could not locate anything specific, so we are turning to the list
for advice. The films are home-made and approx 25-30 nm thick for the formvar and approx 10nm
carbon. thank you-
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From: microscopy.listserver-at-gmail.com
Date: Mon, 5 Mar 2018 19:55:35 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW:CSHL_Career_Opportunity_Research_Associate_=c2=96_?=

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Email: walbrid-at-cshl.edu Name: Samantha Walbridge

Organization: Cold Spring Harbor Laboratory

Title-Subject: [Filtered] CSHL Career Opportunity

Message:
Research Associate – Electron Microscopy Technologist

Cold Spring Harbor Laboratory seeks a highly motivated dedicated individual to work in a
state-of-the-art Microscopy Shared Resource.

The individual should have extensive practical expertise in biological sample preparation for
transmission and scanning electron microscopy of animal tissues and mammalian cell lines. Hands-on
knowledge of confocal and widefield fluorescence microscopy would also be a plus.

The candidate will help users design innovative experiments and they will carry out sample
preparation and imaging as well as assist in data interpretation.

Excellent verbal and written communication skills, ability to work with multiple users in a
supporting role, and ability to work independently and proactively with limited supervision are
essential. A Bachelor’s degree in biology or related discipline is required. One to three years of
experience working in a Microscopy Shared Resource is preferred.

How to Apply

Interested individuals should apply for this position via the CSHL careers website at
http://cshl.peopleadmin.com/postings/11688

Position Number 01779-R

Applicants should include a resume along with a description of their practical expertise and the
names as well as email addresses of 3 references.

Cold Spring Harbor Laboratory is a world-renowned research and educational institution recognized
internationally for its excellence in ground-breaking research programs in cancer, neuroscience,
plant biology, genomics, and bioinformatics and broad educational mission.

For more information about CSHL, please visit us at www.cshl.edu

CSHL is an EO/AA Employer. All qualified applicants will receive consideration for employment and
will not be discriminated against on the basis of race, color, religion, sex, sexual orientation,
gender identity, national origin, age, disability or protected veteran status.

VEVRAA Federal Contractor

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From: microscopy.listserver-at-gmail.com
Date: Wed, 7 Mar 2018 22:48:42 -0600
Subject: [Microscopy] viaWWW: Osmium and UA question

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute

Title-Subject: [Filtered] Osmium and UA question

Message: Dear listers.
My first question is- Is osmium better purchased made up in vials or made from crystals in the lab.
I myself prefer the vials, I feel they are safer and the solution is protected from degradation.

Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased
from Polysciences.

Thank you in advance.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 7 Mar 2018 22:49:50 -0600
Subject: [Microscopy] viaWWW: Princeton-Nature Conference: Frontiers in Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Email: nyao-at-princeton.edu Name: Nan Yao

Organization: Princeton University
Title-Subject: [Filtered] Princeton-Nature Conference: Frontiers in Electron Microscopy for the
Physical and Life Sciences

Message: I would like to ask your help for including the news of a new Nature Conference in electron
microscopy, in your future news update. Website link:
http://www.nature.com/natureconferences/fempl2018/index.html

This new conference is entitled “Princeton – Nature Conference: Frontiers in Electron Microscopy for
the Physical and Life Sciences”, to be held at Princeton, New Jersey, July 11-13, 2018. Many
internationally renowned scientists including 2017 Nobel Prize winner Dr. Joachim Frank will speak
in this conference.

Thank you for your attention. Your help in posting this in the MSA community is greatly appreciated.

Best regards,

Nan Yao


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From: peter.heimann-at-uni-bielefeld.de
Date: Thu, 8 Mar 2018 08:16:15 -0600
Subject: [Microscopy] Re: Osmium and UA question ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
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=} Osmium: I have the impression that 4% OsO4 (stored in sealed glass
ampoules under nitrogen at 4 Celsius) staines less strong after storage
for over 20 years (!); the crystalline form workswell after storage for
more than 20 years (small tip for dissolving: I snap off the tip of the
glass vial, add the water, seal tip with a little amount of
parafilm/nescofilm and sonicate in in ultrasonic waterbath for ca 5
minutes(in fumehood))


=} Uranylacetate: I use UA bought in the early nineties, works perfectly...

greetings,
Peter Heimann
( Cell Biology; Bielefeld University; Germany

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} My first question is- Is osmium better purchased made up in vials or made from crystals in the lab.
} I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Mar 2018 08:46:05 -0600
Subject: [Microscopy] Fwd: Re: viaWWW: Osmium and UA question

Contents Retrieved from Microscopy Listserver Archives
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X-from: Juan Luis Ribas {jlribas-at-us.es}

Dear Buchsmith,

In our hands, we always work with 1 g Osmium crystal (sealed ampoules). As we have a strong sample
preparation load in the lab,  the final solution (1-2%) is consumed in the next month aproximately,
but I suppose that it is very stable.

In Uranile case, we have been using the powder from a bottle with more than 20 years without notice
any degradation related to a new one.

In my opinion, the two substances are very stable over time.

Best regards

Juan Luis


El 08/03/2018 a las 6:08, microscopy.listserver-at-gmail.com escribió:
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} I myself prefer the vials, I feel they are safer and the solution is protected from degradation.
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From: microscopy.listserver-at-gmail.com
Date: Thu, 8 Mar 2018 21:25:08 -0600
Subject: [Microscopy] viaWWW: Osmium and UA question

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X-from: GANG NING {gxn7-at-psu.edu}

Hi - want to share my experience with UA. About 10 yrs ago, I inherited a bottle of UA dated 1960s.
I used it to do girds staining and negative staining. It did stain without any noticeable problems
but then I got a new bottle UA of EMS from another PI, with which my staining looked fresher and
less background. The bottom line is fresh US does give better staining. Your two-year-old UA should
just be fine. Greg
----------------------------
Gang (Greg) Ning, Ph.D.
Director, Microscopy Facility
The Huck Institutes of the Life Sciences
The Pennsylvania State University N-048 MSC
University Park, PA 16802 USA
Phone: 814-863-0994
Fax: 914-867-2587
Email: gxn7-at-psu.edu
http://www.huck.psu.edu/facilities/microscopy-cytometry-up

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute

Title-Subject: [Filtered] Osmium and UA question

Message: Dear listers.
My first question is- Is osmium better purchased made up in vials or made from crystals in the lab.
I myself prefer the vials, I feel they are safer and the solution is protected from degradation.

Second question. Does UA power degrade over time? We have a bottle that is 2+ years old, purchased
from Polysciences.

Thank you in advance.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 9 Mar 2018 11:02:10 -0600
Subject: [Microscopy] viaWWW: ORNL Seeks Technical Associate Staff Member - Scanning Probe

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Technical Associate Staff Member -
Scanning Probe Microscopy

Message: Technical Associate Staff Member - Scanning Probe Microscopy

Oak Ridge National Laboratory (ORNL) seeking a Technical Associate Staff
Member to support scanning probe microscopy efforts at the Center for
Nanophase Materials Sciences (CNMS) at Oak Ridge National Laboratory
(ORNL). In this role, you will be part of the Scanning Probe Microscopy
Group, which is a leader in the development and application of scanning
probe microscopies and spectroscopies to image and manipulate materials
functionality in complex materials, ionic and electronic conductors,
molecular assemblies, and nanoscale structures. A suite of 18 commercial
and internally developed atomic force microscopes in ambient and
controlled environments and scanning tunneling microscopes in ultrahigh
vacuum systems are used for internal and user science, with emerging
opportunities in enhanced data analytics and multimodal/chemical imaging.

For more details, including the qualifications for this position, visit:
http://bit.ly/TP-SPM
ORNL is an equal opportunity employer. All qualified applicants,
including individuals with disabilities and protected veterans, are
encouraged to apply.

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From: WHITTAKS-at-si.edu
Date: Fri, 9 Mar 2018 15:07:15 -0600
Subject: [Microscopy] Job Posting- micro-CT Smithsonian Institution

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Email: rvancamp-at-kettering.edu Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S

Message: We need to hasten our use of our ultramictrotome. I have
identified a few of these still in use but, need more input to find
facilities using this instrument on a daily basis that are also willing
to train me in its operation. I have made progress on my own yet, have
only been able to find one facility willing to provide training. I have
also used the manual and made progress yet, I cannot state the manual
for this instrument is well written. Finally, it is best for me to
locate facilities within the Great Lakes region. I have searched for
online training for this and have been unsuccessful.

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Once again I find myself turning to this most amazing of resources. I am casting a wide net for a micro CT operator and need your collective assistance. Many of you are closely associated with your local CT facilities or at least know the individuals providing the services. I am asking if you would be willing to pass this announcement along as I have not yet found a resource like this one in the scientific CT community.


The Smithsonian Institution National Museum of Natural History (NMNH) in Washington DC has an exciting opportunity to add a cone beam X-ray micro-computed tomography (mCT) operator in support of our new micro-CT Lab. The position is currently a 6 month contract with 2 options to extend providing for the scanning and modelling of a wide variety of natural science and museum specimens for the research staff of the NMNH.

The request for quote (RFQ) and the statement of work (SOW) are provided at the following link. Interested parties need to submit not later than COB April 13th, 2018 to be considered.

https://naturalhistory.si.edu/rc/temp/SImCToperatorRFQ-07032018.pdf

Thank you,

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
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9, 26 -- From WHITTAKS-at-si.edu Fri Mar 9 15:07:14 2018
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9, 26 -- From: "Whittaker, Scott" {WHITTAKS-at-si.edu}
9, 26 -- To: "Whittaker, Scott" {WHITTAKS-at-si.edu}
9, 26 -- Subject: Job Posting- micro-CT Smithsonian Institution
9, 26 -- Thread-Topic: Job Posting- micro-CT Smithsonian Institution
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From: microscopy.listserver-at-gmail.com
Date: Sat, 10 Mar 2018 08:38:31 -0600
Subject: [Microscopy] viaWWW: Position Open Associate Research Scientist - Electron

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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt

Organization: Texas A&M University

Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy

Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences
at Texas A&M University seeks a highly motivated dedicated individual to work in a state-of-the-art
microscopy shared resource.

The individual must have extensive practical expertise in biological sample preparation for
transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of
widefield fluorescence microscopy and confocal and would also be a plus.
Excellent verbal and written communication skills and the ability to work with multiple users are
essential. A doctoral degree in biology or related discipline is required.
A minimum of three years of relevant experience in electron microscopy is required.

Interested individuals should apply for this position via the Texas A&M University website
https://tamus.wd1.myworkdayjobs.com/TAMU_External

Position Number: R-003044

Applicants should include a resume along with a description of their practical expertise and contact
information for 3 references.
Texas A&M University is an Equal Opportunity / Affirmative Action / Veterans / Disability Employer.



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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Mar 2018 14:30:42 -0500
Subject: [Microscopy] viaWWW: Associate Research Scientist - Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: rcburghardt-at-gmail.com

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Email: rcburghardt-at-gmail.com Name: Robert C. Burghardt

Organization: Texas A&M University

Title-Subject: [Filtered] Associate Research Scientist - Electron Microscopy

Message: The Image Analysis Laboratory of the College of Veterinary
Medicine & Biomedical Sciences at Texas A&M University seeks a highly
motivated dedicated individual to work in a state-of-the-art microscopy
shared resource.

The individual must have extensive practical expertise in biological
sample preparation for transmission electron microscopy of animal
tissues and mammalian cell lines. Hands-on knowledge of widefield
fluorescence microscopy and confocal and would also be a plus.
Excellent verbal and written communication skills and the ability to
work with multiple users are essential. A doctoral degree in biology or
related discipline is required.
A minimum of three years of relevant experience in electron microscopy
is required.

Interested individuals should apply for this position via the Texas A&M
University website https://tamus.wd1.myworkdayjobs.com/TAMU_External

Position Number: R-003044

Applicants should include a resume along with a description of their
practical expertise and contact information for 3 references.
Texas A&M University is an Equal Opportunity / Affirmative Action /
Veterans / Disability Employer.



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From: microscopy.listserver-at-gmail.com
Date: Mon, 12 Mar 2018 14:31:56 -0500
Subject: [Microscopy] viaWWW: time on a TEM in the DFW area

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Email: snkeller-at-techie.com Name: Sandra Keller

Organization: TA-Sicco

Title-Subject: [Filtered] TEM time?

Message: Hi All:
I am looking to rent time on a TEM in good condition within a few hours
of the DFW metroplex as soon as possible. The TEM that we normally use
is down at the moment and I am looking for an instrument to use as a
backup. I have many years of TEM experience and would require minimal
training.
Thanks in advance
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Mar 2018 07:33:27 -0500
Subject: [Microscopy] viaWWW:Job at Stanford University - Transmission Electron Microscopy

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] Job Opening at George Washington University

Message: The George Washington University Nanofabrication and Imaging
Center (GWNIC) is seeking a Laboratory Associate or Technician dependent
on experience and qualifications. Duties will include preparation and
imaging of biological and materials samples for microscopy from our
research labs. Additional duties will include general laboratory
management. This is a support position in the GWNIC, for all of the
departments of the University and outside users, where we provide high
quality microscopy services. The minimum qualifications are as follows:
Laboratory Associate: A BA/BS in a related discipline plus 2 years of
relevant professional experience or, a Master’s degree or higher in a
relevant area of study. Degree must be conferred by the start date of
the position. If you are interested in this position, apply at:
http://www.gwu.jobs/postings/49493.
Laboratory Technician: A high school diploma/GED plus 1.5 years of
relevant professional experience, or, a Bachelor’s degree or higher in a
relevant area of study. Degree must be conferred by the start date of
the position. If you are interested, apply at:
http://www.gwu.jobs/postings/49611.

The George Washington University is an Equal Employment
Opportunity/Affirmative Action employer that does not unlawfully
discriminate in any of its programs or activities on the basis of race,
color, religion, sex, national origin, age, disability, veteran status,
sexual orientation, gender identity or expression, or on any other basis
prohibited by applicable law.


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From davishal182newi-at-gmail.com Tue Mar 13 06:56:38 2018
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Email: tobi-at-stanford.edu Name: Tobias Beetz

Organization: Stanford University

Title-Subject: [Filtered] Job at Stanford University - Transmission Electron Microscopy Scientist

Message: Transmission Electron Microscopy Scientist, Stanford Nano Shared Facilities
The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM)
scientist to lead the operations of the facilities’ FEI Titan Environmental TEM. The TEM scientist
will provide training and support to researchers, maintain and optimize the microscope operation,
work closely with equipment vendors, maintain and develop training procedures, develop and implement
advanced techniques, assist and advice users on specimen preparation and data analysis, provide
proof-of-concept service, and engage in research activities. The SNSF Titan ETEM is equipped with a
spherical aberration corrector, EELS and EDS, a monochromator and high brightness gun, Lorentz,
holography, and tomography capabilities, as well as a suite of in situ holders. S(he) will work to
utilize this range of advanced techniques to the highest extent, making them known to the user
community, matching appropriate techniques to the individual research projects. S(he) will interact
with the broader research community and equipment vendors to be aware of advances in the field, and
make recommendations and prepare proposals for future equipment purchases. S(he) will promote and
collaborate in the publication and presentation of results and participate in conferences.
Stanford’s shared nanofacilities offer a comprehensive array of advanced nanofabrication and
nanocharacterization tools. Over 1,000 researchers make use of the shared facilities each year in
order to further their research programs. The goal of the shared facilities at Stanford University
is to provide open, cost-effective access to state-of-the-art nanofabrication and
nanocharacterization facilities for scientists and engineers from academia, small and large
companies, and government laboratories. The FEI Titan TEM is organized under SNSF in the Electron &
Ion Microscopy Suite which currently features a FEI Tecnai TEM as well as two FIB/SEMs and two SEMs.
S(he) may supervise student trainers. The TEM scientist will report to the Faculty Director of SNSF.

For more information about SNSF, visit http://snsf.stanford.edu.

Application Deadline: Applications must be submitted by April 30, 2018.

https://stanford.taleo.net/careersection/2/jobdetail.ftl?job=76088

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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Mar 2018 08:03:15 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Training Inquiry Re: Leica Reichert

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Taylor, Jordan {J.W.Taylor-at-massey.ac.nz}

Hi


I would think that you could get a lesson on any microtome and be able to use this one - especially
if it was one in the Leica family. I learnt on the Ultracut R and didn't need any training to step
up to a EM UC 7. I wouldn't limit my learning oppurtunity to just this model of microtome if I were
you - they are all essentially the same. Success will come with time and experience.


Maybe if you let the listserver know what problems you were having someone may be able to suggest a
work through for you.


Best of luck!


Jordan Taylor
Microscopy Technician
Manawatu Microscopy and Imaging Centre
Massey University
Private Bag 11-222
Palmerston North, 4442
Ph +64 6 356 9099 extn 84719


----------------------------------------------------------------------------------------------------
*From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
*Sent:* Saturday, 10 March 2018 6:27:08 a.m.
*To:* Taylor, Jordan
*Subject:* [Microscopy] viaWWW:Training Inquiry Re: Leica Reichert Ultracut S



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Email: rvancamp-at-kettering.edu Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Training Inquiry Re: Leica Reichert Ultracut S

Message: We need to hasten our use of our ultramictrotome. I have
identified a few of these still in use but, need more input to find
facilities using this instrument on a daily basis that are also willing
to train me in its operation. I have made progress on my own yet, have
only been able to find one facility willing to provide training. I have
also used the manual and made progress yet, I cannot state the manual
for this instrument is well written. Finally, it is best for me to
locate facilities within the Great Lakes region. I have searched for
online training for this and have been unsuccessful.

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25, 54 -- Ultracut S
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From: vau-at-ufl.edu
Date: Tue, 13 Mar 2018 10:20:22 -0500
Subject: [Microscopy] epoxy resin TTE and MBMCA

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Hello All,

I am planning to prepare lung tissue for TEM and Scanning Transmission X-ray Microscopy. An article by Jian Li (2009 Macromolecules 42) A new Approach to Studying Microcapsule Wall Growth Mechanisms stated they used an epoxy resin trimethylolpropane triglycidyl ether (TTE) and 4,4′-methylenebis(2-methylcyclohexylamine) (MBMCA) in a 1:1 weight ratio.

Is anyone familiar with this product? Is there a kit or would one need to purchase the reagents individually from Sigma etc?

Thank you for your suggestions
Karen Kelley, UFL



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3, 43 -- Subject: epoxy resin TTE and MBMCA
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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Mar 2018 07:37:20 -0500
Subject: [Microscopy] viaWWW:ORNL Seeks Postdoc, Scanning Probe Microscopy of Ferroic

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Email: millerme-at-ornl.gov Name: Madalynn Miller

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] ORNL Seeks Postdoc, Scanning Probe Microscopy of Ferroic Materials in
Ultra-High Vacuum

Message: Postdoctoral Research Associate, Scanning Probe Microscopy of Ferroic Materials in
Ultra-High Vacuum

Oak Ridge National Lab’s Center for Nanophase Materials Science (CNMS) is looking for a Postdoctoral
Research Associate to support research in thin film oxide and layered materials within the Scanning
Probe Microscopy group. Our goal is to synthesize films, then measure and tune their structural,
electronic, and chemical properties with scanning probe and electron spectroscopy methods. As a
postdoc, you will contribute to research in these areas using STM imaging and spectroscopy in UHV
along with PLD growth. Research will focus on compositional and surface chemical influences on
structure and piezoelectric response in ferroic materials, particularly oxides.
For more details, including the qualifications and application for this position, visit:
http://bit.ly/ScanProbe

ORNL is an equal opportunity employer. All qualified applicants, including individuals with
disabilities and protected veterans, are encouraged to apply.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 14 Mar 2018 07:38:00 -0500
Subject: [Microscopy] =?UTF-8?Q?viaWWW:SCSMM_symposium_=c2=96April_13=2c_2018_=c2=96Call_?=

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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope

Title-Subject: [Filtered] SCSMM symposium –April 13, 2018 –Call for Abstracts and Image contest

Message: Dear Fellow Microscopists:
This is to announce that Southern California Society for Microscopy and Microanalysis (SCSMM) 2018
spring symposium is to be held on Friday April 13, 2018 at the MCB/Michelson Center for Convergent
Bioscience in the main campus of University of Southern California. Speakers include Mary Scott (UC
Berkeley), Clodagh O’Shea (Salk Institute), Rebecca Voorhees (Caltech), Chongming Wang (Pacific
Northwest National Laboratory), and Cristina Zavaleta (USC). In order to register, please sign up
on-line at http://www.imri.uci.edu/content/2018-spring-meeting-registration no later than 5 p.m.
Friday, April 6.
Annual regular membership $25 (student $10). On-line registration is required.
At the symposium we will have both student platform talk and poster presentations. Please send
abstracts to Sergey Prikhodko (prikhodko.sergey-at-gmail.com) by March 18, 2018.
This year we continue to hold the SCSMM Image Contest. Please send your images to Poorna
Subramanian (poorna.physics-at-gmail.com) by April 6, 2018.
We look forward to seeing you all at the symposium.
Sincerely,
SCSMM board
www.scsmm.org
https://www.facebook.com/microscopymicroanalysis


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From: frank_karl-at-ardl.com
Date: Wed, 14 Mar 2018 10:16:25 -0500
Subject: [Microscopy] SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.

We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.

I'm open to thoughts.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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10, 59 -- Subject: SEM filament
10, 59 -- Thread-Topic: SEM filament
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From: frank_karl-at-ardl.com
Date: Wed, 14 Mar 2018 11:08:10 -0500
Subject: [Microscopy] filament and bad typing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Oppsss.. Ohh damn!
I meant to say our Amray is not set up for field emission.

Sorry about the confusion........
Frank



We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is ( correction add NOT) set up for field emission.

We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.

I'm open to thoughts.




Stay safe.....

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



==============================Original Headers==============================
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From: John.Mardinly-at-asu.edu
Date: Wed, 14 Mar 2018 11:32:23 -0500
Subject: [Microscopy] Stephen Hawking Dies at 76!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://www.bbc.com/news/science-environment-43398187

A. John Mardinly, Ph.D., P.E.

The Sleep of Reason Produces Monsters
Francisco Goya





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From: diller-at-stefan-diller.com
Date: Wed, 14 Mar 2018 13:34:22 -0500
Subject: [Microscopy] Re: SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank,

a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called
"Spitzenkathode".

Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more
coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through
ion bombardment or burn the filament very easily.

I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a
LaB6 cathode to bring all the advantages...

Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...


Best wishes,

Stefan


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
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++49-175-7177051 Mobile

Websites:
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www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
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www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com:
} ----------------------------------------------------------------------------
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}
} We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
}
} We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
}
} I'm open to thoughts.
}
}
}
}
} Stay safe...........
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}
}
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From: colijn.1-at-osu.edu
Date: Wed, 14 Mar 2018 13:36:52 -0500
Subject: [Microscopy] filament and bad typing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank et al,

It sounds like those are pointed tungsten filaments. In the days of
prehistory (aka pre-FEG) in order to get a high brightness/coherence
source people used pointed W cathodes. In order to get the tip of the
needle hot enough to have reasonable emission, the hairpin ran very hot
with the corresponding reduction in lifetime. If I remember, cathode
lifetimes of ~10 hours were common. They would have been used for
high-resolution SEM or high-coherence TEM imaging.

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
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From: mike.marko.em-at-gmail.com
Date: Wed, 14 Mar 2018 14:45:09 -0500
Subject: [Microscopy] SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

I restored an Elmiskop Ia and I have a few boxes of original
Spitzenkathode. They give much more coherence -- really impressive!
But of course they don't last very long.

Does anyone know of another Elmiskop Ia still in operation?

-- Mike


On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote:
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} Hi Frank,
}
} a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called
} "Spitzenkathode".
}
} Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more
} coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through
} ion bombardment or burn the filament very easily.
}
} I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a
} LaB6 cathode to bring all the advantages...
}
} Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...
}
}
} Best wishes,
}
} Stefan
}
}
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} Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com:
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} } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
} }
} } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
} }
} } I'm open to thoughts.
} }
} }
} }
} }
} } Stay safe...........
} }
} } Frank Karl
} } Microscopist
} } Akron Rubber Development Laboratory
} } 2887 Gilchrist Road
} } Akron, Ohio 44305
} }
} }
} }
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From: John.Mardinly-at-asu.edu
Date: Wed, 14 Mar 2018 16:26:30 -0500
Subject: [Microscopy] Fwd: filament and bad typing

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There is extensive discussion of pointed filaments in John Spence’s classic textbook: “Experimental High-resolution Electron Microscopy”, Oxford University Press, 1981 pp. 258-261.
}
} A. John Mardinly, Ph.D., P.E.
} The Sleep of Reason Produces Monsters
} Francisco Goya
}
}
}
} } On Mar 14, 2018, at 11:53 AM, colijn.1-at-osu.edu wrote:
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} }
} } Hi Frank et al,
} }
} } It sounds like those are pointed tungsten filaments. In the days of
} } prehistory (aka pre-FEG) in order to get a high brightness/coherence
} } source people used pointed W cathodes. In order to get the tip of the
} } needle hot enough to have reasonable emission, the hairpin ran very hot
} } with the corresponding reduction in lifetime. If I remember, cathode
} } lifetimes of ~10 hours were common. They would have been used for
} } high-resolution SEM or high-coherence TEM imaging.
} }
} } Cheers,
} } Henk
} }
} }
} } --------------------
} }
} } Hendrik O. Colijn
} } Center for Electron Microscopy and AnalysiS
} } The Ohio State University
} } 1305 Kinnear Rd, Suite 100, Columbus, OH 43212
} }
} } colijn.1-at-osu.edu 614/643-3458
} } cemas.osu.edu
} }
} } "Time is that quality of nature which keeps things from happening all at
} } once." (Ray Cummings - 1922)
} } Lately it doesn't seem to be working.
} }
} } ------ Original Message ------
} } X-from: frank_karl-at-ardl.com
} } To: colijn.1-at-osu.edu
} } Sent: 3/14/2018 12:10:25 PM
} } Subject: [Microscopy] filament and bad typing
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} } }
} } } Oppsss.. Ohh damn!
} } } I meant to say our Amray is not set up for field emission.
} } }
} } } Sorry about the confusion........
} } } Frank
} } }
} } }
} } }
} } } We were recently examining a box of Amray SEM W filaments and found
} } } what we think is an odd feature on some of them. The filament is your
} } } basic loop or hairpin filament, but some have a small needle extending
} } } from one side of the wire at the apex of the point. The needed would
} } } point down the column and has the look of purposeful manufacturing.
} } } It's been suggested these are field emission filaments. Our Amray is (
} } } correction add NOT) set up for field emission.
} } }
} } } We are having trouble with filament drift in use and short lifetimes
} } } but we are reluctant to use these.
} } }
} } } I'm open to thoughts.
} } }
} } }
} } }
} } }
} } } Stay safe.....
} } }
} } } Frank Karl
} } } Microscopist
} } } Akron Rubber Development Laboratory
} } } 2887 Gilchrist Road
} } } Akron, Ohio 44305
} } }
} } }
} } }
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} } 12, 126 -- Subject: Re: [Microscopy] filament and bad typing
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==============================Original Headers==============================
3, 97 -- From John.Mardinly-at-asu.edu Wed Mar 14 16:26:30 2018
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3, 97 -- From: John Mardinly {John.Mardinly-at-asu.edu}
3, 97 -- To: MSA {Microscopy-at-Microscopy.com}
3, 97 -- Subject: Fwd: [Microscopy] filament and bad typing
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From: kb2612-at-columbia.edu
Date: Wed, 14 Mar 2018 17:39:43 -0500
Subject: [Microscopy] Post Doctoral Position at Columbia University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Applications are invited for Postdoctoral Research Scientist position in
the area of thin film deposition and characterization by x-ray
diffraction and electron microscopy at Columbia University. The films
will be deposited by sputtering in an ultrahigh vacuum deposition
chamber. The deposited films will be characterized by X-ray
diffraction, scanning electron microscopy conventional and
high-resolution transmission and scanning transmission electron
microscopy as well as tomographic imaging, analytical electron
microscopy and crystal orientation mapping. This is a full-time
position and the work will be carried out in the thin film deposition
lab, the shared materials characterization and electron microscopy
facilities at Columbia University and at the ASRC of the City University
of New York. The use of facilities at Brookhaven National Laboratories
is also anticipated.

For more details please see http://apam.columbia.edu/katayun-barmak

--
Katayun Barmak
Philips Electronics Professor
Director, Materials Science and Engineering Program
Department of Applied Physics and Applied Mathematics
Seeley W. Mudd Building
Columbia University
500 West 120th Street, Suite 200, MC 4701
New York, NY 10027-6623
Tel: (212) 854-8267
Fax: (212) 854-8257
Email: katayun.barmak-at-columbia.edu
URL: http://apam.columbia.edu/katayun-barmak


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From: diller-at-stefan-diller.com
Date: Thu, 15 Mar 2018 02:35:09 -0500
Subject: [Microscopy] Re: SEM filament

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Mike,

I did own an Elmiskop II and an Elmiskop 101 in the 80ies and 90ies.

Still I have a lot of special gaskets and o-rings, aperture holders and documents on these TEMs. Also tools etc...

If you or anybody else out there reviving an old SIEMENS machine needs parts, contact me ;-)

And: I still have some of the "Spitzenkathodes" and a lot of the normal ones.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 14.03.18 um 21:10 schrieb mike.marko.em-at-gmail.com:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hi Listers,
}
} I restored an Elmiskop Ia and I have a few boxes of original
} Spitzenkathode. They give much more coherence -- really impressive!
} But of course they don't last very long.
}
} Does anyone know of another Elmiskop Ia still in operation?
}
} -- Mike
}
}
} On 3/14/2018 2:56 PM, diller-at-stefan-diller.com wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi Frank,
} }
} } a lot of years ago Siemens manufactured for the ELMISKOP TEMs cathodes with this kind of "tip". In German it`s called
} } "Spitzenkathode".
} }
} } Theoretically it should have some extra luminosity and may deliver - since the beam comes from a very small origin - more
} } coherent electrons. It is known that these tips need a very good vacuum at the cathode, otherwise you will round the tip through
} } ion bombardment or burn the filament very easily.
} }
} } I suppose it is kind of unstable during heating up and will shift a lot. In my opinion it should be centred and mounted like a
} } LaB6 cathode to bring all the advantages...
} }
} } Lifetime had been only part of what is known from a "normal" W filament, maybe 10 hours or so...
} }
} }
} } Best wishes,
} }
} } Stefan
} }
} }
} } -----------------------------------------------------
} } Stefan Diller - Scientific Photography
} } Arndtstrasse 22
} } D - 97072 Wuerzburg Germany
} } ++49-931-7848700 Phone
} } ++49-931-7848701 Fax
} } ++49-175-7177051 Mobile
} }
} } Websites:
} } www.nanoflight.info
} } www.stefan-diller.com
} } www.electronmicroscopy.info
} } www.elektronenmikroskopie.info
} } www.zwillingsprojekt.de
} } www.assisi.de
} } Anfahrt: http://Mail.map24.com/Stefan.Diller
} } -----------------------------------------------------
} }
} } Am 14.03.18 um 16:41 schrieb frank_karl-at-ardl.com:
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } } ----------------------------------------------------------------------------
} } }
} } } We were recently examining a box of Amray SEM W filaments and found what we think is an odd feature on some of them. The filament is your basic loop or hairpin filament, but some have a small needle extending from one side of the wire at the apex of the point. The needed would point down the column and has the look of purposeful manufacturing. It's been suggested these are field emission filaments. Our Amray is set up for field emission.
} } }
} } } We are having trouble with filament drift in use and short lifetimes but we are reluctant to use these.
} } }
} } } I'm open to thoughts.
} } }
} } }
} } }
} } }
} } } Stay safe...........
} } }
} } } Frank Karl
} } } Microscopist
} } } Akron Rubber Development Laboratory
} } } 2887 Gilchrist Road
} } } Akron, Ohio 44305
} } }
} } }
} } }
} } } ==============================Original Headers==============================
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12, 29 -- From diller-at-stefan-diller.com Thu Mar 15 02:35:08 2018
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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Mar 2018 07:51:29 -0500
Subject: [Microscopy] Post-Doctoral Position in Thin Film Deposition and Structural

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Barmak K. {kb2612-at-columbia.edu}



Post-Doctoral Position in Thin Film Deposition and Structural Characterization by X-ray Diffraction
and Electron Microscopy

The Department of Applied Physics and Applied Mathematics at The Fu Foundation School of Engineering
and Applied Science of Columbia University in the City of New York invites applications for a
Postdoctoral Research Scientist position in the area of thin film deposition and characterization by
x-ray diffraction and electron microscopy.The films will be deposited by sputtering in an ultrahigh
vacuum deposition chamber.The deposited films will be characterized by X-ray diffraction, scanning
electron microscopy conventional and high-resolution transmission and scanning transmission electron
microscopy as well as tomographic imaging, analytical electron microscopy and crystal orientation
mapping.This is a full-time position and the work will be carried out in the thin film deposition
lab, the shared materials characterization and electron microscopy facilities at Columbia University
and at the ASRC of the City University of New York.The use of facilities at Brookhaven National
Laboratories is also anticipated.//Candidates for postdoctoral research positions come to the
University to continue their training, generally within three years of completion of Ph.D., for
independent careers as scientists and scholars and must display strong research potential in their
field of study. All candidates are expected to be able to work well in a team and to communicate
effectively the results of their research or research activities both orally and in writing.

Minimum Qualifications:A PhD (or equivalent professional degree) in Materials Science and
Engineering or a related field is required.

The candidate should have actual experience in operation and maintenance of sputtering systems, in
x-ray diffraction studies of polycrystalline and epitaxial films, in scanning electron microscopy,
and in conventional and high-resolution transmission, scanning transmission and analytical electron
microscopy and crystal orientation mapping studies for physical sciences.Experience with tomographic
imaging and image reconstruction is also highly desirable. The microscopy studies will be conducted
on metallic films and lines.The candidate is required to prepare the necessary electron transparent
samples for study using a variety of techniques such as conventional grinding, polishing and ion
milling, chemical back etching, grid transfer techniques for 2D materials, as well as focused ion
beam preparation.The candidate must be able to run experiments and analyze data independently.

Please send your CV, a cover letter and a list of three references with their contact information to
Prof. Barmak at kb2612-at-columbia.edu {mailto:kb2612-at-columbia.edu} .The position is open immediately
and will remain open until filled.

Columbia University is an Equal Opportunity/Affirmative Action Employer.

--
Katayun Barmak
Philips Electronics Professor
Director, Materials Science and Engineering Program
Department of Applied Physics and Applied Mathematics
Seeley W. Mudd Building
Columbia University
500 West 120th Street, Suite 200, MC 4701
New York, NY 10027-6623
Tel: (212) 854-8267
Fax: (212) 854-8257
Email:katayun.barmak-at-columbia.edu
URL:http://apam.columbia.edu/katayun-barmak


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12, 54 -- Subject: Post-Doctoral Position in Thin Film Deposition and Structural
12, 54 -- Characterization by X-ray Diffraction and Electron Microscopy
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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Mar 2018 17:58:45 -0500
Subject: [Microscopy] viaWWW: Electron Microscopy Sciences Microscopy Academy April

Contents Retrieved from Microscopy Listserver Archives
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X-from: stacie-at-ems-secure.com

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Name: Stacie Kirsch

Organization: Electron Microscopy Sciences Microscopy Academy

Title-Subject: [Filtered] APRIL WORKSHOPS - SIGN UP NOW!

Message: APRIL WORKSHOPS - SIGN UP NOW!

MATERIALS ULTRAMICROTOMY Workshop - led by Helmut Gnaegi
Three days of hands-on training for technicians, researchers, and students who want to apply a
faster and cleaner preparation method that provides samples with uniform thickness, no embedded
contamination, and is cheaper than a FIB.

CRYO SEM Workshop - led by Michael Kostrna and Al Coritz
Learn the process of rapid freezing, fracturing, coating and imaging of a variety of samples.
For individuals who are new to the field of cryo SEM, or desire a technical refresher to maintain
current skills, or just those that want to see and learn all of the possibilities of the technology.

VISIT www.emsdiasum.com/academy

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From: vau-at-ufl.edu
Date: Fri, 16 Mar 2018 11:51:46 -0500
Subject: [Microscopy] need help understanding hydrogel morphology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All,

We recently installed a Quorum cryo-SEM prep system to our Hitachi SU5000 SEM for imaging hydrogels. So far, the research groups I've been working with are not pleased with the results. I'm sure it's not the system but more so the lack of hydrogel structural knowledge seen by Cryo-SEM. Is there anyone in this group that can provide guidance? I can send images with our methodology.

hydrogels examined: HA-based hydrogels (GM, MA, 3CG, 3CM, GMHA) and Magnetically templated hydrogels

Thank you
Karen Kelley, UFL




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From: nicholas.conoan-at-unmc.edu
Date: Fri, 16 Mar 2018 15:30:21 -0500
Subject: [Microscopy] Consistent background issues in negative staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello All:

Recently, I have been running a lot of negative staining of nanoparticles for the users of my core facility. A lot of the time, the particles are polymer spheres around 50nm in diameter. The issue is, if I spot just the negative stain (we use NanoVan) onto the grid with nothing else, I see a background of spherical, electron translucent "particles" with a dark border that are consistently between 20 and 40nm. The background is everywhere and at a fairly high density. I have images that I can share via personal communication with anyone who may be interested. With the background being so similar to many of the samples I work with, it is an unacceptable problem.

I believe I have narrowed down the source of said background. It is consistently there if I use brand new forceps, different and/or no pipettor, different stain altogether (2% uranyl acetate), or a new package of grids. However, if I glow discharge the grid immediately before use, the background disappears and I see a clean, smooth, stain as I should.
This leads me to believe that there is either a particle left on the grid surface after manufacturing, or there are "patches" of non-uniform charge that are affecting the stain. The grids we are using are Formvar/ Silicon Monoxide coated 200 mesh copper grids from Ted Pella.

Has anyone else seen this type of background before, or does anyone have any ideas on what it might be? I plan to contact Ted Pella to see if they have any ideas as well.

Thank you, have a great weekend!

-Nick

Nicholas Conoan
Electron Microscopy Specialist
Wittson Hall 2014
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7292


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: nathaniel.rieders-at-montana.edu
Date: Mon, 19 Mar 2018 11:50:19 -0500
Subject: [Microscopy] Secondary Electron Energy Database

Contents Retrieved from Microscopy Listserver Archives
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Greetings,

I am wondering if there is an open access database containing raw data of the secondary emission of electrons from various pure metal surfaces. The raw data would be something like the number of electrons N(E) versus the energy in the range from say 0-100eV.

Thank You,
Nathaniel Rieders

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From: tbargar-at-unmc.edu
Date: Wed, 21 Mar 2018 16:53:29 -0500
Subject: [Microscopy] creating a grid point in Image J

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Hi Listers,

I'm using Image J for some stereology work. It seems that I can't create a grid smaller than 1 square micrometer. Can anyone out there help?

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: microscopy.listserver-at-gmail.com
Date: Sun, 25 Mar 2018 20:20:27 -0500
Subject: [Microscopy] viaWWW: How to deal with outdated computer for your TEM, SEM and

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X-from: itbae-at-binghamton.edu


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Email: itbae-at-binghamton.edu Name: In-Tae Bae

Organization: State University of New York at Binghamton

Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools

Message: Dear Fellow microscopists,
I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM
and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I
contacted each manufacture for a new computer, they usually recommend a newer versions of software
with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based
computer anymore.)
I have more that 10 tools whose computers are almost 10 years old and am looking for a more
affordable way to replace these computers.

I wonder if anyone who deals with the same issue could enlighten me.

Thank you.

In-Tae
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From: vray-at-partbeamsystech.com
Date: Mon, 26 Mar 2018 07:26:01 -0500
Subject: [Microscopy] Re: viaWWW: How to deal with outdated computer for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Aya Takase {Aya.Takase-at-rigaku.com}


Have you considered using an X-ray microscope? You can run scans at room temperature in air.

Aya


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, March
23, 2018 9:56 PM
To: Aya Takase {Aya.Takase-at-rigaku.com}

X-from: Nessler, Randy A {randy-nessler-at-uiowa.edu}

We attempted this in the low vacuum mode of our Hitachi S-3400N, without luck. We resorted to using
the cryostage.
Best,
Randy

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Sent: Friday, March 23, 2018 1:22 PM
To: Nessler, Randy A

Dear  In-Tae Bae,

There is a good reason why OEMs don't want to deal with Xp computers - replacing PCs is simpler and easier then maintaining older
models, and being money-making organizations OEMs must recoup costs of developing and manufacturing upgrades with
associated overhead; mentioned by you cost for computer/SW upgrade between US$10K to US$20K is actually quite reasonable,
considering amount of design effort put into it.

That being said - you can reduce cost of dealing with forced obsolescence and refusal of service by trading money for maintenance effort.

First and foremost, make backup copies of hard-drives on your PCs, since software is the most "non-replaceable" part of the
instrument and the HD failure is not "if," but "when" event.  Then get online, scour websites of second-tier sellers,
get necessary computer hardware (motherboards, processors, memory, peripheral cards, etc...), and build backup PCs
for each and every instrument you have. If you have the expertise and time to do the work yourself, then virtually any
PC can be rebuilt for much less then $1K in cost of parts. If you use one of the decent third-party support organizations,
then cost of labor/travel would increase overall price, but is likely to still be lower then the price of new replacement PC from
the OEM.

Alleged problem with Xp network security due to dropped support is easy to bypass by placing a cheap Win10 PC, or a Linux box,
as a firewall between your Xp PC controlling the instrument and institutional network; images are passed through from
instrument to network, but any other access to/from Xp PC is blocked.

Truly proprietary cards in PCs do pose some problem, although not all instruments have them. If there is such a card found,
then depending on the level of technical expertise available you can either (a) try buying a card from OEM or third-party
support organization, if available, or (b) monitor second-hand equipment market and snatch a PC from similar instrument
when it comes up somewhere, or (c) rely on component-level repair to restore original card when and if it fails, (d) clone
the proprietary card, many of them are quite simple, or (e) bite a bullet and upgrade PC by paying the OEM - which most of
academic and institutional users do anyway.

Best Wishes,
Valery

Valery Ray
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 - leave a message
Mobie: +1-978-305-0479 - leave a message
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com


From:
"microscopy.listserver-at-gmail.com"
{microscopy.listserver-at-gmail.com}

To:
vray-at-partbeamsystech.com
Sent: Sunday, March 25,
2018 9:20 PM
Subject: [Microscopy]
viaWWW: How to deal with outdated computer for your TEM, SEM
and




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Email:
itbae-at-binghamton.edu
Name: In-Tae Bae

Organization:
State University of New York at Binghamton

Title-Subject:
[Filtered] How to deal with outdated computer for your TEM,
SEM and other tools

Message:
Dear Fellow microscopists,
I
have been dealing with an issue that quite a number of
computers (that are hooked up to TEM, SEM
and
other tools) get outdated (and obsolete) while tools are
more or less in good shape. When I
contacted each manufacture
for a new computer, they usually recommend a newer versions
of software
with a
newer computer that easily cost over $10K. (They simply
don't deal with Window XP based
computer anymore.)
I have more that 10 tools
whose computers are almost 10 years old and am looking for a
more
affordable way to
replace these computers.

I wonder if
anyone who deals with the same issue could enlighten me.

Thank you.

In-Tae
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From: microscopy.listserver-at-gmail.com
Date: Sunday, 25March, 2018 at 21:42 To: Philip Oshel
Subject: [Microscopy] viaWWW: How to deal with outdated computer for your TEM, SEM and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------
Mon, 26 Mar 2018 12:22:46 +0000
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

X-from: itbae-at-binghamton.edu


This Question/Comment was submitted to the Microscopy Listserver
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Email: itbae-at-binghamton.edu Name: In-Tae Bae

Organization: State University of New York at Binghamton

Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools

Message: Dear Fellow microscopists,
I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM
and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I
contacted each manufacture for a new computer, they usually recommend a newer versions of software
with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based
computer anymore.)
I have more that 10 tools whose computers are almost 10 years old and am looking for a more
affordable way to replace these computers.

I wonder if anyone who deals with the same issue could enlighten me.

Thank you.

In-Tae
Login Host: 128.226.88.12


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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Mar 2018 08:58:33 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: How to deal with outdated computer for

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------
Mon, 26 Mar 2018 12:22:28 +0000
X-from: Carol Heckman {heckman-at-bgsu.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}


X-from: Henk Colijn {colijn.1-at-osu.edu}

Many times the issue with the custom PC cards is that they plug into an outdated bus. There are
companies that still make motherboards and chassis components for the older interfaces. If the
computer is failing but the cards are still OK, you can get a new PC system and "just" plugin the
cards. It may require some fiddling to get things to work but at least you can get a few more years
from your system.

I just did a web search and found "Nixsys.com" that sells ISA bus PCs. "Cyberresearch.com" also has
passive backplanes with ISA slots. There is no motherboard per se; you add a processor card to the
system. (I have no interest in either company)

Cheers,
Henk

--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.

------ Original Message ------
X-from: microscopy.listserver-at-gmail.com
To: colijn.1-at-osu.edu
Sent: 3/26/2018 9:27:46 AM


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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Mar 2018 22:55:41 -0500
Subject: [Microscopy] Re: viaWWW: How to deal with outdated computer for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------
X-from: James M. Ehrman {jehrman-at-mta.ca}

On a related note, our new (2016) Hitachi SEM and Oxford EDS are banned from accepting Windows 7
updates (by Hitachi) - updates beyond the Windows installation they provided will apparently break
the software. I have them connected together via a router, with both being denied Internet access by
giving them a bogus Default Gateway in the Local Area Connection setup. Another computer (with
Internet access granted) is also connected to the router, and we get data from the two instruments
out to the real world through drive shares. Not elegant, but it works. And I have to say that having
two computers that never nag about updates or suddenly become broken when M$ screws up some driver
file that the instrument depends on is quite refreshing!

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

"It's 5:50 a.m., Do you know where your stack pointer is?"


On 3/26/2018 10:26 AM, microscopy.listserver-at-gmail.com wrote:
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} -------- Forwarded Message --------
} Mon, 26 Mar 2018 12:22:28 +0000
} X-from: Carol Heckman {heckman-at-bgsu.edu}
} To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
} Subject: Re: [Microscopy] viaWWW: How to deal with outdated computer for your TEM, SEM and
}
}
}
} We just linked the old XP machines to another computer running Windows 7 which is secure.  I don't
} know exactly how to do this - check with your IT department.  They can probably fix you up.
}
} Carol Heckman
}
} Bowling Green State University
}
}
}
} ----------------------------------------------------------------------------------------------------
} *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
} *Sent:* Sunday, March 25, 2018 9:47 PM
} *To:* Carol Heckman
} *Subject:* [Microscopy] viaWWW: How to deal with outdated computer for your TEM, SEM and
}
}
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} Email: itbae-at-binghamton.edu Name: In-Tae Bae
}
} Organization: State University of New York at Binghamton
}
} Title-Subject: [Filtered] How to deal with outdated computer for your TEM, SEM and other tools
}
} Message: Dear Fellow microscopists,
} I have been dealing with an issue that quite a number of computers (that are hooked up to TEM, SEM
} and other tools) get outdated (and obsolete) while tools are more or less in good shape. When I
} contacted each manufacture for a new computer, they usually recommend a newer versions of software
} with a newer computer that easily cost over $10K. (They simply don't deal with Window XP based
} computer anymore.)
} I have more that 10 tools whose computers are almost 10 years old and am looking for a more
} affordable way to replace these computers.
}
} I wonder if anyone who deals with the same issue could enlighten me.
}
} Thank you.
}
} In-Tae
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From: microscopy.listserver-at-gmail.com
Date: Mon, 26 Mar 2018 22:56:26 -0500
Subject: [Microscopy] Fwd: How to deal with outdated computer for

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Reinhard Rachel {Reinhard.Rachel-at-biologie.uni-regensburg.de}

} } } 26.03.2018 at 15:26:
} We just linked the old XP machines to another computer running Windows 7
} which is secure. I don't
} know exactly how to do this ‑ check with your IT department. They can
} probably fix you up.

the tricky thing is that even Windows 7 will not be supported (by MS) any more
in 1 or 2 years time ... we will not have fun with "old" computer systems. regards - Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29 Next microscopy conferences:
- 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
http://imc19.com/
- Microscopy Conference MC2019: 1.-5. Sept 2019 in Berlin - next Microbiol. conferences: VAAM -
Annual Conf.: 15-18 April 2018 Wolfsburg


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From: frank_karl-at-ardl.com
Date: Tue, 27 Mar 2018 12:25:47 -0500
Subject: [Microscopy] method induced differences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,
The story goes, there once was a fella who ran EDS on a rubber sample and found less 1% (semi quant mode). But another lab under the corporate umbrella used XRF as well as ICP and found 5%.

I got my theories and hand waving, but I can't really help my co-worker out. I'm open to theories. What does the collective wisdom of the microscopy community say?

Thanks........

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: wesaia-at-iastate.edu
Date: Tue, 27 Mar 2018 17:57:32 -0500
Subject: [Microscopy] method induced differences

Contents Retrieved from Microscopy Listserver Archives
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You didn't say, but I am guessing you meant they were analyzing for sulfur. I will presume so for this discussion.

If you were measuring S in rubber, that would mean a lot of C in the matrix. I would REALLY doubt the C number from semi-quant mode. C may have been dreadfully inflated and thus would have diluted the S content. Some systems may be better than others but I am always surprised when the C comes out even halfway close to right in semi-quant mode.

I would rather try to measure C(+H) by difference. That would mean going somewhat beyond semi-quant mode. You would have to turn off normalization and calibrate your beam intensity to that used to collect the standards. Specify C as the element to be determined by difference. I think the resulting S number would be much more reasonable. (At least it was when I did my MS work nearly 40 years ago. They accepted it for my thesis work.) I know how to do that on Oxford equipment today, and I had done that on Noran equipment for my masters. I don't know how the others would set that up.

Warren Straszheim

I only got 22 out-of-office messages to my last post. What will I find this time?

-----Original Message-----
X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com]
Sent: Tuesday, March 27, 2018 12:27 PM
To: Straszheim, Warren E [BIOTC]

Hello Everyone,
The story goes, there once was a fella who ran EDS on a rubber sample and found less 1% (semi quant mode). But another lab under the corporate umbrella used XRF as well as ICP and found 5%.

I got my theories and hand waving, but I can't really help my co-worker out. I'm open to theories. What does the collective wisdom of the microscopy community say?

Thanks........

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: Eugene.CHOO-at-oxinst.com
Date: Tue, 27 Mar 2018 21:10:50 -0500
Subject: [Microscopy] method induced differences

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

My advice to you is to trust the XRF and ICP results over the EDS.

It is always a big problem to perform quantification on a non-conductive material such as rubber in SEM.
Under the typical SEM conditions, the electron beam will induce localized charging on the scanned region of interest.
Charging is a big problem and causes unpredictable data in the EDS spectrum.
Also to get an accurate quantification in EDS, you have to make sure you acquire a statistically significant amount of X-ray counts as recommended by the EDS manufacturer.
Finally, EDS is also a surface sensitive technique and so you have to make sure that the region of interest is not coated with some additional layer foreign material (such as powder or grease)

Most layman users also stick to the easy-way-out Normalized method of quantification, which is another problem if you have source of uncertainty in other elements.
As pointed out by Warren, one element that tends to build up over time is Carbon.
Hence, assuming Normalized quantification was used, the data will appear to have higher C%, and resulting in lower S% for example.

In conclusion, if the EDS data was not acquired by a well-trained SEM-EDS user, the result should not be trusted with high degree of confidence.
You should advise your co-worker to contact the local support for the EDS provider to confirm whether data acquisition methodology was applied correctly.
I am also open to discussion on the parameters you used for acquiring the EDS data on the rubber samples.


Eugene
eugene.choo-at-oxinst.com


-----Original Message-----
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu]
Sent: Wed, 28 Mar, 2018 7:23 AM
To: CHOO Eugene

You didn't say, but I am guessing you meant they were analyzing for sulfur. I will presume so for this discussion.

If you were measuring S in rubber, that would mean a lot of C in the matrix. I would REALLY doubt the C number from semi-quant mode. C may have been dreadfully inflated and thus would have diluted the S content. Some systems may be better than others but I am always surprised when the C comes out even halfway close to right in semi-quant mode.

I would rather try to measure C(+H) by difference. That would mean going somewhat beyond semi-quant mode. You would have to turn off normalization and calibrate your beam intensity to that used to collect the standards. Specify C as the element to be determined by difference. I think the resulting S number would be much more reasonable. (At least it was when I did my MS work nearly 40 years ago. They accepted it for my thesis work.) I know how to do that on Oxford equipment today, and I had done that on Noran equipment for my masters. I don't know how the others would set that up.

Warren Straszheim

I only got 22 out-of-office messages to my last post. What will I find this time?

-----Original Message-----
X-from: frank_karl-at-ardl.com [mailto:frank_karl-at-ardl.com]
Sent: Tuesday, March 27, 2018 12:27 PM
To: Straszheim, Warren E [BIOTC]

Hello Everyone,

The story goes, there once was a fella who ran EDS on a rubber sample and found less 1% (semi quant mode). But another lab under the corporate umbrella used XRF as well as ICP and found 5%.

I got my theories and hand waving, but I can't really help my co-worker out. I'm open to theories. What does the collective wisdom of the microscopy community say?

Thanks........

Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Mar 2018 08:56:32 -0500
Subject: [Microscopy] viaWWW: Repairing a Zeiss EM 109

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Email: kathleen.g.roberts-at-rutgers.edu Name: KATHLEEN ROBERTS

Organization: RUTGERS UNIVERSITY

Title-Subject: [Filtered] Repairing a Zeiss EM 109

Message: To all,

We have a Zeiss EM 109 that is acting up. Is there a company (or someone) that is reasonably local
to Rutgers (Piscataway) who can come look at it? Our previous repair person retired.

Thank you so much for all your help,
Kathleen

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14, 53 -- Subject: viaWWW: Repairing a Zeiss EM 109
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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Mar 2018 08:57:32 -0500
Subject: [Microscopy] viaWWW: AFM Scientist Job Opening

Contents Retrieved from Microscopy Listserver Archives
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Email: dartjulia-at-bfusa.com Name: Julia

Organization: Bridgestone

Title-Subject: [Filtered] AFM Scientist Job Opening
Message: Dear all,

Bridgestone Americas Center for Research and Technology is currently seeking an experienced Research
Scientist with expertise in Microscopy to join an interdisciplinary team of scientists and engineers
working to solve problems at the leading edge of tire technology.

Ph.D. in Material Science, Chemistry, Physics, or a related technical discipline in chemistry or
other science related disciplines. Ability to independently lead research combining advanced
analytical method development with material science, polymer science, and tire technology needs.
Extensive hands on experience in AFM, SEM, TEM, quantitative image analysis, and statistical
analysis is desired. Location: Akron, OH

More information and to apply:

https://bridgestoneamericas.jobs/akron-oh/scientist-iii-microscopy/0834E464=
3319449C84EC180868B8B7D4/job/


Regards,
Julia


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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Mar 2018 08:58:31 -0500
Subject: [Microscopy] rviaWWW: Research Scientist /Senior Research Scientist - Electron

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Email: m.aindow-at-uconn.edu Name: Mark Aindow

Organization: University of Connecticut

Title-Subject: [Filtered] Research Scientist /Senior Research Scientist - Electron Microscopy

Message: Tech Park, University of Connecticut

Position ID: UConn-TechPark-2018299 [#10893, 2018299]
Position Title: Research Scientist /Senior Research Scientist - Electron Microscopy
Position Type: Non tenure-track faculty
Position Location: Storrs, Connecticut 06269, United States [map]
Subject Area: Innovation Management
Appl Deadline: none (posted 2018/02/19)
Position Description: https://academicjobsonline.org/ajo/jobs/10893/apply Reporting directly to
the Executive Director of the Innovation Partnership Building (IPB) at the UConn Tech Park, the
Research Scientist will be engaged in research, securing extramural funding, planning experiments,
evaluating, interpreting and publishing results. This position would have a May 2018 start date, and
will be renewed annually contingent upon research productivity and successful generation of
extramural sponsored projects, including, industry agreements and grant funding.

The successful applicant will be expected to maintain an independent and/or collaborative program of
research supported by extramural funding, including writing proposals, running research projects and
publishing high quality reports and other publications

The incumbent will also: operate and maintain equipment related to Electron Microscopy including
SEM, STEM, TEM, and high resolution TEM ; develop new procedures and modify or custom-design complex
lab equipment and resolve design and malfunction problems of equipment; perform highly specializes
tasks requiring precision and accuracy involving repeatable results; resolve difficult problems with
sophisticated and sensitive lab instruments; Instructs others in the proper and safe use of a
variety of laboratory equipment, material and chemical handling processes, bottled gases and
associated distribution systems, laboratory materials and chemicals, ventilation systems, high
temperature furnaces, presses etc.; and may train and or oversee the work of other staff including
technical support for material characterization instruments at the Innovation Partnership Building

MINIMUM QUALIFICATIONS

PhD in Material Science, Chemistry, Physics or other relevant fields.
Five plus years of postdoctoral or industry experience in a relevant field.
Experience with aberration corrected Electron Microscopy.
Professional interpersonal skills and the demonstrated ability to work effectively as a team player
with a wide variety of individuals.
Strong written and communications skills.
Demonstrated ability to handle multiple tasks and activities while simultaneously ensuring problems
are resolved efficiently and effectively.
PREFERRED QUALIFICATIONS

Significant hands-on experience with aberration corrected Electron Microscopy.
Familiarity with safe handling of materials and chemicals.
Proven troubleshooting and problem-solving capabilities.
Familiarity with developing standard operating procedures.
Familiarity with designing and implementing research protocols.
APPOINTMENT TERMS

This is a full-time, 11-month, annually renewable, non-tenure track position. This position has the
possibility of annual reappointments, contingent upon performance and funding. Employment is
conditional upon the timely completion of an approved I-9 (Employment Eligibility Verification
Form). Rank and salary will be commensurate with qualifications and experience.

TO APPLY

Select “Apply” to be directed to Academic Jobs Online to complete your application. Please submit
the following: a cover letter indicating experience with proposal development; curriculum vitae; and
the names and contact information of three reference writers. Screening of applicants will begin
immediately. Employment of the successful candidates will be contingent upon the successful
completion of a pre-employment criminal background check. (Search # 2018295)

All employees are subject to adherence to the State Code of Ethics which may be found at
http://www.ct.gov/ethics/site/default.asp.

The University of Connecticut is committed to building and supporting a multicultural and diverse
community of students, faculty and staff. The diversity of students, faculty and staff continues to
increase, as does the number of honors students, valedictorians and salutatorians who consistently
make UConn their top choice. More than 100 research centers and institutes serve the University’s
teaching, research, diversity, and outreach missions, leading to UConn’s ranking as one of the
nation’s top research universities. UConn’s faculty and staff are the critical link to fostering and
expanding our vibrant, multicultural and diverse University community. As an Affirmative
Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans,
people with disabilities and members of traditionally underrepresented populations.





Application Materials Required:
Submit the following items online at this website to complete your application:
Cover Letter
Curriculum Vitae
Three References (no actual letters, just names and email addresses help popup)
And anything else requested in the position description.

Further Info:
https://techpark.uconn.edu/
University of Connecticut
Tech Park
159 Discovery Drive
Storrs, CT 06268

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From: ech-at-uvic.ca
Date: Thu, 29 Mar 2018 14:18:32 -0500
Subject: [Microscopy] The Great Lego Microscope Competition

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The Great Lego Microscope Competition

Two years ago, Tina Carvalho put on the list server “How do I make my smart phone into a microscope?” She got lots of replies. I have been collecting quite a lot of them. My favorites will be at the Outreach Booth at M&M in Baltimore in August. Some work better than others and a lot of the results depends on how good your smart phone camera is. I still use the Echo Wooden Microscope with my smart phone when I take a plankton tow.

I wondered how difficult it would be to make a Lego version. I borrowed some Lego from my son and made one. However, the permutations of what is possible with Lego seemed extensive, so I am putting forward “The Great Lego Microscope Competition”.

Place: M&M Meeting, Baltimore 2018, Outreach Booth
Lenses: the designer has to take care of.  Simple lenses work fine for this project.
Technical Lego can be used.
Prizes: yes

I will have the version I made, and I hope I have given you enough time to put something together to bring to the meeting.

I will bring some Lego to one of the stations in “Microscopic Explorations” (used to be Family Affair), on the Wednesday afternoon, to make a Lego Microscope. While I am on the subject of Microscopic Explorations, which is for delegates, their families and friends, this year we should have “how to put together a Foldscope, and activities to use with the Foldscope including a couple of solve the mysteries.” All attendees should take away their own put-together Foldscope and be comfortable using it.

Best wishes
Elaine


Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada

Lab: 250-853-3968
cell: 250-886-2068
website: http://www.stehm.uvic.ca




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From: microscopy.listserver-at-gmail.com
Date: Fri, 30 Mar 2018 17:31:41 -0500
Subject: [Microscopy] rviaWWW: The Great Lego Microscope Competition

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Email: duraine-at-bcm.edu Name: Lita Duraine

Organization: Howard Hughes Medical Institute

Title-Subject: [Filtered] RE: [Microscopy] The Great Lego Microscope Competition

Message: A LEGO microscope would be cool.

In 2017 there was a push for the LEGO factory to make a LEGO Transmission Electron and Scanning
Electron Microscopes (not working of course). People could vote for it. Here is the web page:
https://ideas.lego.com/projects/102b2832-4574-4870-95a0-8698211d3fdf

Not sure what happened to the idea, haven't seen any in the stores. But it would be neat to have in
the lab.
Lita

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From: microscopy.listserver-at-gmail.com
Date: Fri, 30 Mar 2018 17:32:43 -0500
Subject: [Microscopy] viaWWW: Liposome with An Nanopaticle attached

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Email: ravi.thakkar369-at-gmail.com Name: Ravi

Organization: Ravi Thakkar

Title-Subject: [Filtered] Liposome with An Nanopaticle attached.

Message: Hi all,

I have user having liposome sample attached with Au nanoparticle by additional functional group and
its control sample (liposome and Nanoparticle) without functional group attached. Both samples look
great under TEM.

But how to identify the Au Nanoparticles are attached to Liposomes by functional group or randomly
seated on liposome. If anyone here can give some idea.

Thanks.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 31 Mar 2018 08:24:24 -0500
Subject: [Microscopy] viaWWW: How to deal with outdated computer for your

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Steve Chapman {protrain-at-emcourses.com}

Hi

On a lightly different matter.

The problem of out of date EM computers has been going on for many years and
when training service technicians I encouraged then to educate the customer
on this. At the time of an EM installation the laboratory will usually be
flooded with PC, so we suggest that when replacing that equipment the
laboratory keeps a couple of the redundant units complete with keyboard and
mouse as back up spares.
How often when training operators did we have a failure, fan, transformers,
keyboard etc, when with redundant PC available we were up and running again
in no time. Being prepared is a good idea.
Regards

Steve

Steve Chapman FRMS
Protrain for Consultancy and Training in Electron Microscopy
+44 (0)7711 606967
web www.emcourses.com


-----Original Message-----
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[mailto:microscopy.listserver-at-gmail.com] Sent: 26 March 2018 02:22
To: protrain-at-emcourses.com

X-from: itbae-at-binghamton.edu


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Email: itbae-at-binghamton.edu Name: In-Tae Bae

Organization: State University of New York at Binghamton

Title-Subject: [Filtered] How to deal with outdated computer for your TEM,
SEM and other tools

Message: Dear Fellow microscopists,
I have been dealing with an issue that quite a number of computers (that are
hooked up to TEM, SEM and other tools) get outdated (and obsolete) while
tools are more or less in good shape. When I contacted each manufacture for
a new computer, they usually recommend a newer versions of software with a
newer computer that easily cost over $10K. (They simply don't deal with
Window XP based computer anymore.) I have more that 10 tools whose computers
are almost 10 years old and am looking for a more affordable way to replace
these computers.

I wonder if anyone who deals with the same issue could enlighten me.

Thank you.

In-Tae
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From: microscopy.listserver-at-gmail.com
Date: Sat, 31 Mar 2018 13:49:15 -0500
Subject: [Microscopy] Fwd: Dealing with old computers on microscopes

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: John Minter {jrminter-at-gmail.com}



​Steve Chapman's recent note matched our lab practice of keeping a supply of suitable replacement PCs.

I did want to add a couple of suggestions:

1. Use a suitable hard disk backup program (we used an old copy of Norton Ghost) to make a backup
hard drive for your instrument when it is fully configured. This saved our bacon many times. You
will be glad you did because you can spend days rebuilding an instrument PC from scratch. Unless you
are a real packrat, you likey won't have all the service pack installers to get a vanilla install to
where you need to be.

2. Secure network file shares ​that are properly backed up and archived are worth the effort. Create
one. Store everything you need for the instrument in a folder tree for that instrument. I created a
"site book" document for the instrument in the root folder. Add all your notes for maintenance and
sys admin to that document. A wise statistician I know quipped, "Your closest collaborator is you
six months from now. And you don't respond to email." Let me add a couple of examples: 1) what is
the admin password to the UPS unit so I can reset the battery lifetime after I replaced the
batteries to turn of the alarm? 2) What is the part number for the correct filter for the new
chiller? 3) What was that command sequence the service engineer showed me 9 months ago that let me
get to the vacuum diagostic panel to see why my microscope won't pump down properly? You get the idea...

While you are at it save PDF copies of all the manuals and installers for any software you need on
that network share in the appropriate folder.  Save all the electronic copies of the service reports
there. This folder will be your one-stop for everything you need for the instrument. No more
questions of "Where did I file that?" You can set different access levels for different people who
might need to see the info.  Trust me, future you will be very happy that present you did this.

Best regards,
John Minter
Retired microscopist from Kodak Analytical Sciences

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From: John.Mardinly-at-asu.edu
Date: Sun, 1 Apr 2018 13:49:53 -0500
Subject: [Microscopy] Re: Dealing with old computers on microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

John;
Good advice, but even better than Norton Ghost is cloning. I have had service engineers ‘ghost’ hard drives many times, and EVERY time the ghost was needed to restore a system, it failed. For less than the cost of Ghost, you can buy a new hard drive, clone it, and keep the clone around for when something happens to your system disk. I have never seen a clone fail, plus you can test it right away to see if it is good. One note: Acronis recommends never leaving the cloned disk and the original disk connected to the bus; attempting to boot the computer with both drives connected can corrupt the boot sector of the new clone.

A. John Mardinly, Ph.D., P.E.
Also Retired

}
}
}
} -------- Forwarded Message --------
}
} X-from: John Minter {jrminter-at-gmail.com}
}
}
}
} ​Steve Chapman's recent note matched our lab practice of keeping a supply of suitable replacement PCs.
}
} I did want to add a couple of suggestions:
}
} 1. Use a suitable hard disk backup program (we used an old copy of Norton Ghost) to make a backup
} hard drive for your instrument when it is fully configured. This saved our bacon many times. You
} will be glad you did because you can spend days rebuilding an instrument PC from scratch. Unless you
} are a real packrat, you likey won't have all the service pack installers to get a vanilla install to
} where you need to be.
}
} 2. Secure network file shares ​that are properly backed up and archived are worth the effort. Create
} one. Store everything you need for the instrument in a folder tree for that instrument. I created a
} "site book" document for the instrument in the root folder. Add all your notes for maintenance and
} sys admin to that document. A wise statistician I know quipped, "Your closest collaborator is you
} six months from now. And you don't respond to email." Let me add a couple of examples: 1) what is
} the admin password to the UPS unit so I can reset the battery lifetime after I replaced the
} batteries to turn of the alarm? 2) What is the part number for the correct filter for the new
} chiller? 3) What was that command sequence the service engineer showed me 9 months ago that let me
} get to the vacuum diagostic panel to see why my microscope won't pump down properly? You get the idea...
}
} While you are at it save PDF copies of all the manuals and installers for any software you need on
} that network share in the appropriate folder. Save all the electronic copies of the service reports
} there. This folder will be your one-stop for everything you need for the instrument. No more
} questions of "Where did I file that?" You can set different access levels for different people who
} might need to see the info. Trust me, future you will be very happy that present you did this.
}
} Best regards,
} John Minter
} Retired microscopist from Kodak Analytical Sciences
}
} ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Mon, 2 Apr 2018 06:59:57 -0500
Subject: [Microscopy] viaWWW: Issues with Aquila Topcon Hybrid SEM

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Email: Nareh.movsesian-at-usc.edu Name: Nareh Movsesian

Organization: Univeristy of Southern California

Title-Subject: [Filtered] Issues with Aquila Topcon Hybrid SEM

Message: Hello. My name is Nareh and I am a PhD student at University of Southern California. We are
currently having sample transfer issues with our Topcon SEM (Aquila Topcon Hybrid SEM) in the lab.
Looks like there could be issues with the mechanical parts of the instrument that hold and transfer
the sample holder to the stage. These are issues with loading and unloading the samples. We know
this because most of the time the sample doesn't get loaded onto the stage and we are not able to
see it in the OM map. Or if we get lucky and it gets loaded, we encounter issues with unloading the
sample. The option to click on unload and vent the sample on the software is sometimes available and
sometimes not. ( and when it is, the system just vents and comes back to atmospheric pressure
without unloading the sample)

We tried doing some maintenance on the stage parts but haven't seen any improvements and the company
we bought this SEM from hasn't been responding ( maybe out of business...)

We would really appreciate if you have any tips regarding these issues or if you could refer a
company that may be able to fix this

Thanks

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From: lkerr-at-mbl.edu
Date: Mon, 2 Apr 2018 08:17:26 -0500
Subject: [Microscopy] Balzers Freeze-Fracture Service

Contents Retrieved from Microscopy Listserver Archives
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Hi,

We have a 1980's vintage Balzers freeze-fracture instrument (model 300/301?) and we are in search of someone who can service it. If you know of someone, or a company, I would appreciate getting some contact information.

Thanks,
Louie

Louie Kerr | Director, Imaging Services; Staff Scientist
Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543
508-289-7273 | lkerr-at-mbl.edu | www.mbl.edu




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7, 57 -- From: Louis Kerr {lkerr-at-mbl.edu}
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7, 57 -- Subject: Balzers Freeze-Fracture Service
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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Apr 2018 14:28:21 -0500
Subject: [Microscopy] viaWWW:Biological TEM Workshop @ University of Georgia

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Email: johnshields59-at-gmail.com Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Bio TEM Workshop at GEM

Message: Biological TEM Workshop

This intensive, three-day workshop will provide a practical and basic
theoretical introduction to the Transmission Electron Microscope and
biological sample preparation techniques. Each day will consist of
lecture, discussion and *hands-on* training led by GEM staff.
What: Anyone requiring training on TEM and biological sample
preparation. The workshop will be limited to 6 participants based on the
availability of equipment.
When: Wednesday through Friday, July 25-27, 2018, 8am-5pm each day
(lunch is provided)

Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more
information and to sign up. Registration requires iLab account through
the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: July 20, 2018


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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Apr 2018 18:38:23 -0500
Subject: [Microscopy] viaWWW: ODP Circuit Philips CM120

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Email: drademacher-at-luc.edu Name: David Rademacher

Organization: Loyola University Chicago

Title-Subject: [Filtered] ODP Circuit

Message: I came in this morning and observed the following error message on our Philips CM 120: ODP
Circuit. Essentially what is happening is that, upon start-up, the system gets hung up at the very
first step of the vacuum sequence. I am polling the expert audience to determine if anyone has
encountered this issue and to learn what was done about it. Please don't hesitate to contact me
drademacher-at-luc.edu with any comments/suggestions.

Best Regards,

Dave Rademacher
Loyola University Chicago

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From: microscopy.listserver-at-gmail.com
Date: Wed, 4 Apr 2018 21:54:34 -0500
Subject: [Microscopy] viaWWW: The Great Lego Microscope Competition

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: James M. Ehrman {jehrman-at-mta.ca}

I challenged my son to make some Lego scopes about 20 years ago. Here are the results:

http://www.mta.ca/dmf/lego.html

Except for more appropriate stickers on the parts, I think they came out pretty well. Sadly, they
often get more attention from visitors in the lab than the actual equipment does...

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf


On 3/30/2018 7:32 PM, microscopy.listserver-at-gmail.com wrote:
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} Organization: Howard Hughes Medical Institute
}
} Title-Subject: [Filtered] RE: [Microscopy] The Great Lego Microscope Competition
}
} Message: A LEGO microscope would be cool.
}
} In 2017 there was a push for the LEGO factory to make a LEGO Transmission Electron and Scanning
} Electron Microscopes (not working of course). People could vote for it. Here is the web page:
} https://ideas.lego.com/projects/102b2832-4574-4870-95a0-8698211d3fdf
}
} Not sure what happened to the idea, haven't seen any in the stores. But it would be neat to have in
} the lab.
} Lita
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From: benada-at-biomed.cas.cz
Date: Thu, 5 Apr 2018 07:33:35 -0500
Subject: [Microscopy] viaWWW: ODP Circuit Philips CM120

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Dave,
In the Manual, there is written "ODP Circuit - contact service department".

We had this problem long time ago and we have solved it by buying a new ODP heater. We bought a new one from a company in Germany. Unfortunately, I do not have the details at hand now. The heater can be replaced by user. It is not a difficult task.

My best regards

Oldrich

P. S.
Just now I have found the old broken heater ring from our Philips CM100. Its specification is "CHROMALOX CAT NO KB15 VOLTS 210 JB WATTS 450". The heater can be found on the EDWARDS Webshop:

{https://shop.edwardsvacuum.com/products/H01700186/view.aspx}

Please, look at your diffusion pump type if this specification is also valid form your pump.

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Wed, 4 Apr 2018 18:42:40 -0500, microscopy.listserver-at-gmail.com wrote :
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} Email: drademacher-at-luc.edu Name: David Rademacher
}
} Organization: Loyola University Chicago
}
} Title-Subject: [Filtered] ODP Circuit
}
} Message: I came in this morning and observed the following error
} message on our Philips CM 120: ODP Circuit. Essentially what is
} happening is that, upon start-up, the system gets hung up at the very
} first step of the vacuum sequence. I am polling the expert audience
} to determine if anyone has encountered this issue and to learn what
} was done about it. Please don't hesitate to contact me
} drademacher-at-luc.edu with any comments/suggestions.
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} Best Regards,
}
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} Loyola University Chicago
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From: microscopy.listserver-at-gmail.com
Date: Thu, 5 Apr 2018 08:02:54 -0500
Subject: [Microscopy] Fwd: Re: The Great Lego Microscope Competition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Hendricks, Gregory {Gregory.Hendricks-at-umassmed.edu}


Hi Dave,
It sounds like a Water Chiller problem. Your chiller is probably making hot water, maybe good
for tea but not for your microscope.
Greg

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday,
April 4, 2018 7:52 PM
To: Hendricks, Gregory {Gregory.Hendricks-at-umassmed.edu}




-------- Forwarded Message --------

X-from: Andy Stewart {andy.stewart1975-at-gmail.com}


Dear List-serv,

Here are the instructions to build a LEGO highbase Titan Themis we made for the launch of our
microscope.
https://ulsites.ul.ie/mssi/sites/default/files/Titan%20Themis%20Instructions.pdf
{https://ulsites.ul.ie/mssi/sites/default/files/Titan Themis Instructions.pdf}

For those that are interested, here are the two microscopes being built.
https://youtu.be/x6ArO0-CNu4

The launch video of the microscope
https://youtu.be/PADirETav5k

To find those more difficult to source lego pieces I found bricklink.com {http://bricklink.com}  to
be very useful.

Happy Building
Andy

———————————————————————————

Dr Andy Stewart
Department of Physics & Energy
Bernal Institute
University of Limerick
Ireland
+353 (0) 61-23-7733
temul.ie {http://temul.ie}


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From: microscopy.listserver-at-gmail.com
Date: Thu, 5 Apr 2018 08:03:40 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: The Great Lego Microscope Competition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Elaine Humphrey {ech-at-uvic.ca}

Hi James and Lita
I do need to point out that this competition is about making your smart phone into a microscope and
needs to be a working model.

That being said: What a fun way to look at the instruments in your lab. Thank you for sharing James
and thank you Lita.

Roseann Csencsits sent me this link
https://ideas.lego.com/projects/fcce15cd-27e0-405b-990b-681b7787fbc6

I hope to see lots of wonderful models in Baltimore in August.
Elaine


Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada
Lab: 250-853-3968
cell: 250-886-2068
website: http://www.stehm.uvic.ca



On 04/04/2018, 20:09, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:

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-------- Forwarded Message --------
X-from: James M. Ehrman {jehrman-at-mta.ca}
I challenged my son to make some Lego scopes about 20 years ago. Here are the results:
http://www.mta.ca/dmf/lego.html
Except for more appropriate stickers on the parts, I think they came out pretty well.
Sadly, they often get more attention from visitors in the lab than the actual equipment does...
Jim
-- James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA
phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf
On 3/30/2018 7:32 PM, microscopy.listserver-at-gmail.com wrote:
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} X-from: duraine-at-bcm.edu
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} Email: duraine-at-bcm.edu Name: Lita Duraine
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} Organization: Howard Hughes Medical Institute
}
} Title-Subject: [Filtered] RE: [Microscopy] The Great Lego Microscope Competition
}
} Message: A LEGO microscope would be cool.
}
} In 2017 there was a push for the LEGO factory to make a LEGO Transmission Electron and Scanning
} Electron Microscopes (not working of course). People could vote for it. Here is the web page:
} https://ideas.lego.com/projects/102b2832-4574-4870-95a0-8698211d3fdf
}
} Not sure what happened to the idea, haven't seen any in the stores. But it would be neat to
have in
} the lab.
} Lita
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From: microscopy.listserver-at-gmail.com
Date: Thu, 5 Apr 2018 20:33:30 -0500
Subject: [Microscopy] Fwd: Leica Ultracut error

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: John J. Perrino {jperrino-at-stanford.edu}



Hello all,

Our Leica UCT is having an issue, error code 102 with a U_15 in the FEED boxes, which will not allow
me to turn the top lights on initially and now it also takes out the motor drive. I have localized
the issue in the control box having replaced it with a twin Ultracut's control box and had no
issues. The cable is also fine. Any suggestions would be greatly appreciated.

John


John Perrino
Cell Sciences Imaging Facility
279 Campus Drive West
B001 Beckman
Stanford, CA 94305
650-723-3462
Fax 650-725-4951

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From: microscopy.listserver-at-gmail.com
Date: Thu, 5 Apr 2018 20:34:12 -0500
Subject: [Microscopy] Fwd: RE: viaWWW: ODP Circuit Philips CM120

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Ben Micklem {ben.micklem-at-pharm.ox.ac.uk}

I used to look after two CMs until very recently. I think 'ODP circuit' means the heater's electric
circuit is open (i.e. likely the heater element has failed, as someone else mentioned); 'ODP Water'
is when the water temperature just after the ODP reaches 60 degrees C, i.e. water flow or chiller
issue; 'ODP Oil' is triggered when the heater has been running for a while, but the oil hasn't
reached target temperature- I've had this with a working heater but with a colder thank usual water
supply at higher than normal flow rates; but it could also be an issue with the heater.

Ben



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Email: drademacher-at-luc.edu Name: David Rademacher

Organization: Loyola University Chicago

Title-Subject: [Filtered] ODP Circuit

Message: I came in this morning and observed the following error message on our Philips CM 120: ODP
Circuit. Essentially what is happening is that, upon start-up, the system gets hung up at the very
first step of the vacuum sequence. I am polling the expert audience to determine if anyone has
encountered this issue and to learn what was done about it. Please don't hesitate to contact me
drademacher-at-luc.edu with any comments/suggestions.

Best Regards,

Dave Rademacher
Loyola University Chicago

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From: vitalylazar-at-att.net
Date: Fri, 6 Apr 2018 00:32:16 -0500
Subject: [Microscopy] Re: viaWWW: ODP Circuit Philips CM120

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Common problems in order of probability:

1) open ODP heater

2) poor connection at ODP heater terminal (ceramic block located near
bottom of ODP)

3) sticky ODP relay in MS unit (power cabinet, right side, one of large
relays). Watch vacuum screen on data monitor during vacuum system
startup. You must hear relay clicking simultaneously with ODP symbol
highlighting shortly after vac. system start, as soon as P2 reading
drops below 38. (assuming room is quiet)

4) defective water safety thermostat switch on water line coil (middle
of ODP) or poor connection of it. Look at connecting wires, they can be
burnt if touching bottom of ODP.

Other causes possible but unlikely

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 4/4/2018 7:40 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] ODP Circuit
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} Message: I came in this morning and observed the following error message on our Philips CM 120: ODP
} Circuit. Essentially what is happening is that, upon start-up, the system gets hung up at the very
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9, 31 -- To: microscopy.listserver-at-gmail.com,
9, 31 -- Microscopy Listserver {Microscopy-at-microscopy.com} , drademacher-at-luc.edu
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From: microscopy.listserver-at-gmail.com
Date: Fri, 6 Apr 2018 07:16:48 -0500
Subject: [Microscopy] viaWWW: Vector graphics for images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

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Email: holpc-at-firstenergycorp.com Name: Chris Holp

Organization: FirstEnergy - BETA Labs

Title-Subject: [Filtered] Vector graphics for images

Message: Hi everyone,
We are interested in updating our photo editing software, with an eye primarily on good vector
graphics for annotation purposes. I would appreciate receiving thoughts and suggestions; what do you
use, what do you like about it, as well as what may be a little tricky/unsatisfactory (if anything)
about your program.

Private replies are no problem, and thanks in advance!
Chris Holp
Staff Nuclear Specialist
FirstEnergy – BETA Lab
6670 Beta Dr.
Mayfield Village, OH 44143
holpc-at-firstenergycorp.com



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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Apr 2018 08:05:25 -0500
Subject: [Microscopy] viaWWW: Technical Trainer/Instructor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] Technical Trainer/Instructor

Message: Description

Gatan, Inc. is the world's leading manufacturer of instrumentation and software used to enhance and
extend the operation and performance of electron microscopes. The Gatan name is recognized and
respected throughout the worldwide scientific community and has been synonymous with high quality
products and the industry's leading technology.

We are currently seeking an experienced Technical Trainer/Instructor who will be responsible for
organizing and delivering training for our global Service team. This individual will set standards
for Service training, develop technical training curriculum based on company products and customer
needs, and manage documentation and records. Some domestic and international travel will be
involved with this role. This position will be based out of our Pleasanton, CA office.

The incumbent will be responsible for:

• Devise technical training programs and certification tracking per organizational requirements.
• Produce training schedules and classroom agenda.
• Determine course content per objectives.
• Develop new training materials, which may include written documents, video tutorials, and on-line
web based training.
• Arrange for and conduct on-site training when needed.
• Keep and report data on completed courses, issues etc.
• Observe and evaluate results of training programs.
• Determine overall effectiveness of programs and make improvements.
• Train support personnel such as tech support and field service engineers.
• Develop and document technical service procedures.
• Act as a two-way conduit for information transfer between the engineers who are responsible for
designing our products and field service teams who are responsible for product maintenance,
installation and support.
• Gain a systems-engineering level knowledge of highly complex, leading edge systems by working with
the product engineering and service teams.
• The systems include optics, electronics, mechanical components, vacuum systems, custom software
and algorithms.

The successful incumbent will have:

• Experience in course development, training and documentation.
• Work on complex problems where analysis of situations or data requires an in-depth evaluation of
various factors.
• Results-oriented and driven to excel.
• Enjoys teaching, troubleshooting, working with groups of people, and explaining complex things as
simply as possible.
• Quick grasp of challenging, complex technical information.

Fundamental Requirements:

• BS in Physics or Engineering (or equivalent)
• 5+ years related experience; Graduate degree a plus.
• Ability to successfully develop and facilitate technical training and documentation.
• Strong analytic and data analysis skills.
• Excellent oral and written communication skills.
• Ability to work effectively in multifunction, multicultural teams and to take a leadership role
when needed.
• Strong communications and project management skills desired.
• Must have electronic, electromechanical, and vacuum skills in troubleshooting complex systems issues.
• Proven ability to service heavy equipment within acceptable ergonomic standards desired.
• Microscopy imaging/analytical equipment support experience preferred.

To Apply, log onto:
https://recruiting.ultipro.com/ROP1001ROPER/JobBoard/d622ca39-91ce-4093-94dc-0dfda5adec2d/OpportunityDetail?opportunityId=b268136a-c81a-4e50-af94-aaa370b5a5fa

Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities

The contractor will not discharge or in any other manner discriminate against employees or
applicants because they have inquired about, discussed, or disclosed their own pay or the pay of
another employee or applicant. However, employees who have access to the compensation information of
other employees or applicants as a part of their essential job functions cannot disclose the pay of
other employees or applicants to individuals who do not otherwise have access to compensation
information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in
furtherance of an investigation, proceeding, hearing, or action, including an investigation
conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information.


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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Apr 2018 08:06:04 -0500
Subject: [Microscopy] Fwd: [Filtered] MicroscopyListserverviaWWW: Research Associate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: rburghardt-at-cvm.tamu.edu

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Email: rburghardt-at-cvm.tamu.edu Name: Robert C Burghardt

Organization: Texas A&M University

Title-Subject: [Filtered] Research Associate Position - Transmission Electron Microscopy

Message: The Image Analysis Laboratory of the College of Veterinary Medicine & Biomedical Sciences
at Texas A&M University seeks a highly motivated individual to work in a state-of-the-art microscopy
shared resource.

The individual must have extensive practical experience in biological sample preparation for
transmission electron microscopy of animal tissues and mammalian cell lines. Hands-on knowledge of
widefield fluorescence and confocal microscopy would also be a plus.

Excellent verbal and written communication skills and the ability to work with multiple users are
essential. A doctoral degree in biology or related discipline is required.

Interested individuals should apply for this position via the Texas A&M University website
https://tamus.wd1.myworkdayjobs.com/TAMU_External

Position number, R-003044

Applicants should include a resume along with a description of their practical expertise and contact
information for 3 references.

Texas A&M University is and Equal Opportunity / Affirmative Action / Veterans / Disability Employer


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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Apr 2018 08:22:17 -0500
Subject: [Microscopy] viaWWW: Vector graphics for images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Parish, Chad M. {parishcm-at-ornl.gov}

I find Adobe Illustrator to be my favorite choice. Although it costs money, everything is
well-automated and there are tons of help websites, tutorials, and videos online, so that I never
need more than a minute or two to figure out a new command. The ability to do my annotations FAST
more than makes up for the yearly cost of the Adobe CC subscription, given my hourly chargeout rate.
If you want free, Inkscape seems good, but I've not bothered to learn it in detail.

I do all of my analysis in MATLAB and use commands like } save( gcf,'filename.pdf','pdf' ); to save
my MATLAB figures as PDF, which Illustrator will read natively as vector graphics. That's the
fastest, most efficient way to turn data into figures I've found. I imagine Python, etc., have
similar tricks using either PDF or SVG.

(No financial interest -- just a satisfied customer.)

Chad

---------------------
Chad M. Parish, Ph.D.
Research and Development Staff Member
Radiation Effects and Microstructural Analysis Team
Nuclear Materials Science and Technology Group
Materials Science and Technology Division
Oak Ridge National Laboratory
Phone: 1 865 574 0092
Email: parishcm-at-ornl.gov



-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, April
06, 2018 8:32 AM
To: Parish, Chad M.




-------- Forwarded Message --------

X-from: holpc-at-firstenergycorp.com

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using the WWW based Form at http://www.microscopy.com/MicroscopyListserver/MLFormMail.html
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Email: holpc-at-firstenergycorp.com Name: Chris Holp

Organization: FirstEnergy - BETA Labs

Title-Subject: [Filtered] Vector graphics for images

Message: Hi everyone,
We are interested in updating our photo editing software, with an eye primarily on good vector
graphics for annotation purposes. I would appreciate receiving thoughts and suggestions; what do you
use, what do you like about it, as well as what may be a little tricky/unsatisfactory (if anything)
about your program.

Private replies are no problem, and thanks in advance!
Chris Holp
Staff Nuclear Specialist
FirstEnergy - BETA Lab
6670 Beta Dr.
Mayfield Village, OH 44143
holpc-at-firstenergycorp.com



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From: stefan.diller-at-t-online.de
Date: Mon, 9 Apr 2018 08:31:24 -0500
Subject: [Microscopy] Looking for Gatan PIPS 691 or parts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I am looking for a complete Gatan PIPS 691, functional or non-functional, or parts for non-commercial use. I would be happy to pay
a small amount of money and of course for packing and sending.


Thanks,

Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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10, 23 -- Subject: Looking for Gatan PIPS 691 or parts
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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Apr 2018 22:05:02 -0500
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: vera.desmarais-at-einstein.yu.edu

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Email: vera.desmarais-at-einstein.yu.edu Name: Vera DesMarais

Organization: Analytical Imaging facility Einstein College of Medicine
Title-Subject: [Filtered] Job Opportunity

Message: The Analytical Imaging Facility (Microscopy Core facility) at Albert Einstein College of
Medicine in New York is currently looking for an entry level light microscopy technician. Please
follow the link below for a more detailed job description and to apply for the job.

https://careers-einstein.icims.com/jobs/10344/research-technician-b/job?mobile=false&width=950&height=500&bga=true&needsRedirect=false&jan1offset=-300&jun1offset=-240



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From: amit.welcomes.u-at-gmail.com
Date: Thu, 12 Apr 2018 05:38:14 -0500
Subject: [Microscopy] Phase reconstruction of electronic work function

Contents Retrieved from Microscopy Listserver Archives
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I wanted to know if it is possible to get difference in electronic
properties, such as work function (potential needed to extract an
electron), between two materials of similar dimensions, from exit wave
reconstruction.

i.e. suppose I have 2 nano particles, of radius r, both are identical
as such but one have slightly higher wrok function. Can i visualize
that work function difference using exit wave? I am not able to find
exact literature. How ever several tantalizingly close results exist.
such as this- pubs.acs.org/doi/abs/10.1021/acs.nanolett.6b04957

Any nudge is appreciated!

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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 Apr 2018 16:11:34 -0500
Subject: [Microscopy] viaWWW:riggers to move SEM

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Title-Subject: [Filtered] riggers to move SEM

Message: We're getting ready to move to a new facility. I like to know of any companies that
specialize moving SEM's. The instrument will be in moving condition.


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From: zaluzec-at-microscopy.com
Date: Thu, 12 Apr 2018 16:12:22 -0500
Subject: [Microscopy] viaWWW:Summer Courses at EMS Microscopy Academy

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Title-Subject: [Filtered] Summer Courses at EMS Microscopy Academy

Message: Microscopy: The Complete Image (3 weeks)
Add to your skills in Scanning, Transmission, Optical Light, or General Microscopy!
Walk away in three short weeks with the ability to run and be proficient in all aspects of specimen
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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 Apr 2018 16:13:04 -0500
Subject: [Microscopy] viaWWW:ZEISS Regel-transformator

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Email: omelon-at-ku.edu Name: Christopher Omelon

Organization: KU

Title-Subject: [Filtered] ZEISS Regel-transformator

Message: Hi All,

I am looking for a regel-transformator transformer 50-60Hz 30VA Type 39 25 24 for a Zeiss Standard
18 polarizing microscope. Any help is appreciated.

Thanks,

Chris

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From: microscopy.listserver-at-gmail.com
Date: Sat, 14 Apr 2018 06:45:08 -0500
Subject: [Microscopy] viaWWW:NCI Request for Information on development of an Imaging Data

Contents Retrieved from Microscopy Listserver Archives
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Email: jettsd-at-nih.gov Name: Stephen Jett

Organization: National Cancer Institute

Title-Subject: [Filtered] NCI Request for Information on development of an Imaging Data Commons

Message: Hello everyone,

As part of its efforts to increase access to cancer data and data analysis tools, the National
Cancer Institute is establishing a Cancer Research Data Commons (CRDC). Imaging is a crucial area
for analysis and discovery in cancer, so part of this effort is the development of an Imaging Data
Commons (IDC). Given the breadth of the data and tools in cancer imaging, we are seeking input from
the research community to ask their thoughts on the data, tools and features they feel are important
to include in such a data commons. To engage potential contributors, a Request for Information
(RFI) was released to solicit this input. I’ve included below the text of a flyer we’re
distributing to announce the RFI, including the URL to the RFI and the email for
questions/responses. You can also contact me directly with any questions. If you are aware of
similar existing efforts, please feel free to reference those in a response. If you have any
questions regarding the RFI, you may send them to either the NCIIDCRFI-at-mail.nih.gov address or
jettsd-at-nih.gov (they’re both me).
Thank you in advance for any input.
Best regards,
Steve Jett
The NCI is inviting comments and suggestions on the development of the NCI Imaging Data Commons
(IDC), a node of the Cancer Research Data Commons. The IDC will provide:
· access to image repositories
· analysis tools
· scalable computing resource
· a cloud-based, collaborative environment. To best serve the needs of the cancer imaging
community, we are seeking input from potential users of the IDC to determine the best features to
include in an IDC prototype. All stakeholders involved in cancer imaging are invited to respond to
this Request. More details about the RFI and how to respond can be found at
https://grants.nih.gov/grants/guide/notice-files/NOT-CA-18-060.html
The deadline for submission is May 4, 2018.

For any questions about this request, please contact

NCIIDCRFI-at-mail.nih.gov
___________________
Stephen D. Jett, Ph.D.
AAAS Science & Technology Policy Fellow
Center for Biomedical Informatics and Information Technology (CBIIT)
National Cancer Institute, National Institutes of Health
9609 Medical Center Drive
Rockville, MD 20850
Tel.: 240-276-5537
stephen.jett-at-nih.gov

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From: tina-at-pbrc.hawaii.edu
Date: Sun, 15 Apr 2018 18:39:07 -0500
Subject: [Microscopy] About Caroline Schooley

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am sorry to report that my friend and mentor, Caroline Schooley, passed
away early Sunday morning with family members at her side.

When I was a new grad student in 1976, working in a lab looking at
neurosecretion, mostly with electrophysiology, it was suggested to my
advisor that someone learn electron microscopy for the project. So in 1976
I got 'volunteered' and was sent to Berkeley for a summer course led by
Caroline Schooley. At first I was overwhelmed and terrified, but within a
couple of weeks Caroline, with her unique blend of generosity and
gruffness, had gotten me into a better housing situation, introduced me to
people who would become lifelong friends, and fueled my fascination with
microscopy. It didn't take long for me to decide I wanted to make EM a
career, and also that it might be both fun and challenging to run a
multi-user facility like hers where people from all walks of academia,
from science to art, could meet and exchange ideas. She insisted I join
MSA early on, pointing out that the Society was unusual in being gender
blind and also degree blind, welcoming to everyone. Over the years I also
spent time with her family in Berkeley and Mendocino as well as in Kona,
surrounded by literally stacks and stacks of books, enjoying food and
wine, tidepooling, and photography. I used to be shy and reserved, and
uncertain of my place because I was isolated (in those days) on a small
island in the middle of the Pacific. But Caroline encouraged me to become
involved with and serve the Society. This drew me out and gave me a
better sense of self, and no one who knows me can say I'm shy anymore.
Each of us has special people in our lives who have helped to guide us to
where we are. For me, this person was Caroline Schooley.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************

==============================Original Headers==============================
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From: ech-at-uvic.ca
Date: Sun, 15 Apr 2018 19:25:42 -0500
Subject: [Microscopy] Re: About Caroline Schooley

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

When I read about how Caroline mentored Tina, it struck me that was what she did for me too. I met Caroline in the early 2000's at M&M as I was interested in doing more Outreach at home. She had retired from running a busy lab at Berkeley and put a lot of energy into getting ProjectMICRO started. She supported the MSA collaboration with the Lawrence Hall of Science to publish the GEMS book "Microscopic Explorations." When she found it difficult to come to the meetings I agreed to co-chair ProjectMICRO and look after the meeting side. At that time, I knew I did not know enough to do the job properly and it was good that she "had my back." She made sure reports went in on time, boxes of materials for ProjectMICRO arrived on time, I was on time. She knew how to get things done and mentored me along the way. At first, she could seem gruff and maybe intimidating if someone was not listening to her, but her heart was in the right place and it was golden and she was always generous. She knew how things should be done.
I will miss her.
Elaine


Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada

Lab: 250-853-3968
cell: 250-886-2068
website: http://www.stehm.uvic.ca



On 15/04/2018, 16:57, "tina-at-pbrc.hawaii.edu" {tina-at-pbrc.hawaii.edu} wrote:

I am sorry to report that my friend and mentor, Caroline Schooley, passed
away early Sunday morning with family members at her side.

When I was a new grad student in 1976, working in a lab looking at
neurosecretion, mostly with electrophysiology, it was suggested to my
advisor that someone learn electron microscopy for the project. So in 1976
I got 'volunteered' and was sent to Berkeley for a summer course led by
Caroline Schooley. At first I was overwhelmed and terrified, but within a
couple of weeks Caroline, with her unique blend of generosity and
gruffness, had gotten me into a better housing situation, introduced me to
people who would become lifelong friends, and fueled my fascination with
microscopy. It didn't take long for me to decide I wanted to make EM a
career, and also that it might be both fun and challenging to run a
multi-user facility like hers where people from all walks of academia,
from science to art, could meet and exchange ideas. She insisted I join
MSA early on, pointing out that the Society was unusual in being gender
blind and also degree blind, welcoming to everyone. Over the years I also
spent time with her family in Berkeley and Mendocino as well as in Kona,
surrounded by literally stacks and stacks of books, enjoying food and
wine, tidepooling, and photography. I used to be shy and reserved, and
uncertain of my place because I was isolated (in those days) on a small
island in the middle of the Pacific. But Caroline encouraged me to become
involved with and serve the Society. This drew me out and gave me a
better sense of self, and no one who knows me can say I'm shy anymore.
Each of us has special people in our lives who have helped to guide us to
where we are. For me, this person was Caroline Schooley.

Aloha,
Tina

****************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu/bemf*
****************************************************************************




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From: vau-at-ufl.edu
Date: Mon, 16 Apr 2018 14:40:45 -0500
Subject: [Microscopy] immunogold particle count

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Can anyone provide a reference and the acceptable reporting methods for immunogold particle counting?

Thank you
Karen



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From: microscopy.listserver-at-gmail.com
Date: Tue, 17 Apr 2018 08:11:10 -0500
Subject: [Microscopy] viaWWW: CMMS meeting: May 8th, 5 - 8pm, George Washington University

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-------- Forwarded Message --------

X-from: chrisbrantner-at-gwu.edu

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] Chesapeake Society Spring Meeting

Message: CMMS meeting: May 8th, 5 - 8pm, George Washington University
Dear CMMS members,
Please join us for a dinner meeting on May 8th at the George Washington University. Our speaker
that evening will be Dr. Shigeki Watanabe who's talk is entitled "Ultrafast endocytosis of synaptic
vesicles". Dr. Watanabe's talk will be preceded by dinner and a short business meeting. A
printable flyer for the meeting is available here.

The cost for the meeting will be $35 dollars per person. Registration is available here. The
registration deadline is May 4th; we regret that we cannot accept registrations after that date or
at the door.
The business meeting will be particularly important as there are several issues that must be decided:
The Society needs someone to assume the duties (regulatory and tax filings) of the resident agent as
I will be leaving the DC/MD/NoVA area this summer. This must be a Maryland resident as the Society
is currently incorporated in Maryland.
The Society needs at least two people who are interested in helping to organize meetings and events
for the Society to step up and volunteer their time.
Without both of these matters being decided, the future of the Society will be in question so I urge
you to attend this meeting and become involved in the future of the Society.

If you have any questions, please contact me at ChesapeakeMicroscopy-at-gmail.com

I look forward to seeing many of you on the 8th.

Sincerely,
Cam Robinson
President, CMMS

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From: andrei.kolmakov-at-nist.gov
Date: Tue, 17 Apr 2018 14:24:14 -0500
Subject: [Microscopy] postdoc: in situ electron microscopy and spectroscopy in liquids and

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues, please distribute among your students, postdocs and associates.
Thank you
Andrei

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
NIST (PNL/CNST) is expanding its research program toward the development of in situ electron microscopy (SEM, PEEM etc) and spectroscopy (XPS, AES, XAS etc) of objects/processes (incl. bio/medical) and devices in liquids and dense gaseous environments using environmental cells equipped with electron transparent windows (e.g. graphene). Currently, a postdoctoral position for highly motivated and experienced experimentalist is available. The preferable set of skills includes, but not limited to SEM, XPS, AES, PEEM, AFM, u-Raman, CL, UHV surface science techniques, synchrotron radiation and clean room experience, graphene transfer/ functionalization protocols, electrochemistry, excellent writing and teamwork skills and etc.
The application letter, CV with publication record and contact names of 3-4 references should be sent to Dr. Andrei Kolmakov, andrei.kolmakov-at-nist.gov
In case further information is needed, do not hesitate to contact Dr. Andrei Kolmakov at:

NIST 100 Bureau Drive
Bldg. 216/Rm.B117
Gaithersburg, MD 20899-6204
Phone: (301) 975-4724
Fax: (301) 975-2303
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
___________________________________________________
Dr. Andrei Kolmakov
Project Leader, Nanoscale Imaging and Spectroscopy Group
Center for Nanoscale Science & Technology
National Institute of Standards & Technology
100 Bureau Dr. Mail Stop 6203
Gaithersburg, MD 20899
Phone: (301) 975-4724
Fax: (301) 975-2303
Email: andrei.kolmakov-at-nist.gov
URL https://www.nist.gov/people/andrei-kolmakov




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From: microscopy.listserver-at-gmail.com
Date: Tue, 17 Apr 2018 19:44:52 -0500
Subject: [Microscopy] In Memoriam: Dr Richard Crang

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------
X-from: Miller, Lou A {lamiller-at-illinois.edu}

It is with great sadness that that we have heard of of Dr Richard Crang's passing this last Friday.

I am helping John Bozzola post this, he has gathered the information for the following remembrance
of Richard Crang.


Richard Crang will be missed as the mentor, co-worker and friend he was to so many of us here in the
midwest and across the world.

------------------------------------------------------------------------------------------------------------------

Richard Francis Earl Crang

Richard Francis Earl Crang (81) passed away on April 13, 2018 at his home in La Grange Highlands,
IL. He is survived by his wife Mary; two sons, Steven (Diane) and Douglas (Kate); four grandsons,
Alexander, Eliot, Evan and Nathaniel; and three adult step-sons. He was a graduate of Eastern
Illinois University, and received his masters degree at the University of South Dakota and his
Ph.D. in Botany at the University of Iowa. He later carried out a year of post-doctoral work in
Botany and Physiology at Clare Hall of the University of Cambridge, England.
He held academic positions at Wittenberg University (Springfield, OH), Bowling Green State
University (OH), the University of Illinois at Urbana-Champaign (UIUC), and The City University of
New York. At UIUC, he served for 12 years as the Director of the Center for Electron Microscopy;
two years as Associate Head of the Department of Plant Biology; and over two years as a Faculty
Fellow in the Office of the Vice President for Academic Affairs.
Dr. Crang received an Alfred P. Sloan Foundation Fellowship to support his pioneering work in the
development of an online course and electronic textbook in Plant Anatomy. He later guided
development of the first online academic courses in Nursing and Pharmacy at the University of Illinois.
He served for several years as a member and Chair of the National Research Council graduate
fellowship committee for the National Science Foundation. He was sponsored by the Federal EPA and
the US Department of State to carry out research work at the Komarov Botanical Institute in St.
Petersburg (formerly Leningrad) Russia during the 1980s. He also held residential research
appointments in microscopy at the Max Planck Institute for Molecular Physiology in Dortmund, Germany
and at the Swiss Federal Institute of Technology in Zurich, Switzerland.
Dr. Crang is the only U.S. Plant Biologist to be awarded Fellowship status by the International
Society of Environmental Botanists. While at Bowling Green State University, he was the first
recipient of the Universitys Outstanding Research Award. Later, Dr. Crang was named the second
recipient of the Outstanding Service Award of the Electron Microscopy Society of America.
He has been the author/co-author of six textbooks, over 70 academic papers, and over one hundred
abstracts, short papers and submitted contributions in the fields of microscopy and plant biology.
His most recent work has been as co-author for a just-completed comprehensive textbook on Plant Anatomy.
As Professor Emeritus, he carried out humanitarian missionary work in Colombia and was on the
instructional faculty at the Pyongyang University of Science and Technology in North Korea.
Relentlessly curious, he traveled to 41 countries where he eagerly engaged with local people and
enthusiastically explored local cultures.
Visitation with the family will be Tuesday, April 17, 2018 from 1:00 P.M. to time of service 2
P.M. at Hitzeman Funeral Home & Cremation Services, 9445 W. 31st St., Brookfield, Illinois 60513.
In lieu of flowers, the family asks that donations be made to the Plant Biology Annual Fund in the
Dept. of Plant Biology at UIUC in Dr. Crangs name at sib.illinois.edu/alumni/donations or
Department of Plant Biology, 265 Morrill Hall, MC-116, 505 South Goodwin Avenue, Urbana, IL 61801.
Information 708-485-2000 or www.HitzemanFuneral.com



{ { { { { { { { { { { { { { { { {} } } } } } } } } } } } } } } }
Lou Ann Miller
Biological Electron Microscopy
Frederick Seitz Materials Research Laboratory
Lab Room 125 Office 374 MRL
104 South Goodwin Ave
Urbana, IL 61801
Campus mail code: MC-230
Work Hours: 7am - 3:30 pm

217-244-1567
lamiller-at-illinois.edu
http://mrl.illinois.edu


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From: wpchan-at-uw.edu
Date: Thu, 19 Apr 2018 23:52:10 -0500
Subject: [Microscopy] HT on a Philips CM100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi

Can I please get your advice on a problem with the HT on a Philips CM100?

When I press the HT On/Off button to turn on the high tension, I got no
response, i.e., I cannot turn on the high tension. The vacuum are good,
both HiV and UHV lights are on. I pulled the power supply (A7) and found
that the fuses are intact. The voltage going into A7 is 227V. I also
measured the resistance across the HT On/Off push button. It is 12 ohm when
pressed and 0 ohm released.

Is there anything obvious that I have missed? Thanks!


--
Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519)
The Biology Imaging Facility (http://depts.washington.edu/if/)

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From: benada-at-biomed.cas.cz
Date: Fri, 20 Apr 2018 01:15:41 -0500
Subject: [Microscopy] Re: HT on a Philips CM100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Pang,
There is a security switch "S34" on CM100. You can find it on the left side of the column on the lifting assembly. Check it if it is closed.
If it is open, the HT cannot be switched on.

Regards

Oldrich

--
Oldřich Benada
Institute of Microbiology CAS, v.v.i.
Laboratory of Molecular Structure Characterization
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Thu, 19 Apr 2018 23:58:44 -0500, wpchan-at-uw.edu wrote :
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} Hi
}
} Can I please get your advice on a problem with the HT on a Philips
} CM100?
}
} When I press the HT On/Off button to turn on the high tension, I got
} no response, i.e., I cannot turn on the high tension. The vacuum are
} good, both HiV and UHV lights are on. I pulled the power supply (A7)
} and found that the fuses are intact. The voltage going into A7 is
} 227V. I also measured the resistance across the HT On/Off push
} button. It is 12 ohm when pressed and 0 ohm released.
}
} Is there anything obvious that I have missed? Thanks!
}
}
} --
} Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519)
} The Biology Imaging Facility (http://depts.washington.edu/if/)
}
} ==============================Original
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From: microscopy.listserver-at-gmail.com
Date: Sun, 22 Apr 2018 20:06:16 -0500
Subject: [Microscopy] viaWWW:Job opportunity at OHSU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you everyone for all the helpful tips. I'll try them out and
report back. Have a great weekend.

--
Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519)
The Biology Imaging Facility (http://depts.washington.edu/if/)


} On Thu, 19 Apr 2018 23:58:44 -0500, wpchan-at-uw.edu wrote :

} } Hi
} }
} } Can I please get your advice on a problem with the HT on a Philips
} } CM100?
} }
} } When I press the HT On/Off button to turn on the high tension, I got
} } no response, i.e., I cannot turn on the high tension. The vacuum are
} } good, both HiV and UHV lights are on. I pulled the power supply (A7)
} } and found that the fuses are intact. The voltage going into A7 is
} } 227V. I also measured the resistance across the HT On/Off push
} } button. It is 12 ohm when pressed and 0 ohm released.
} }
} } Is there anything obvious that I have missed? Thanks!

==============================Original Headers==============================
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-------- Forwarded Message --------

X-from: lopezcl-at-ohsu.edu

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Email: lopezcl-at-ohsu.edu Name: Claudia Lopez

Organization: OREGON HEALTH & SCIENCE UNIVERSITY

Title-Subject: [Filtered] Job opportunity at OHSU

Message: Dear microscopy community,
The Multiscale Microscopy Core (MMC), located on Oregon Health and Science University’s South
Waterfront Campus is currently looking for an electron microscopy technician at the Research
Assistant 2 level. The MMC
(http://www.ohsu.edu/xd/research/research-cores/multi-scale-microscopy-core/index.cfm) is a state of
the art electron microscopy university core that provides imaging, training and technical support to
researchers in the Pacific Northwest. The Research Assistant 2 will provide support to the MMC’s
activities by preparing and processing samples and assisting the team in meeting its goals. For more
information please visit OHSU’s job website using the IRC code “IRC68462”.
Kind regards,

Claudia

Claudia S. López, PhD
Research Assistant Professor
Department of Biomedical Engineering
Director Multiscale Microscopy Core
Phone: 503-418-0186




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From: microscopy.listserver-at-gmail.com
Date: Mon, 23 Apr 2018 07:24:46 -0500
Subject: [Microscopy] Fwd: FIB-SEM job opening at the Advanced Imaging Center, HHMI Janelia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Chew, Teng-Leong {chewt-at-janelia.hhmi.org}

Hi all,

The Advanced Imaging Center (AIC) at the Howard Hughes Medical Institute Janelia Research Campus and
Janelia Group Leader Dr. Harald Hess are working together to introduce focused ion beam scanning
electron microscopy (FIB-SEM) to the imaging center. We are actively looking for a research
specialist/application scientist. The FIB-SEM we are building is custom-designed to be able to
operate seamlessly for very long-term milling/imaging experiment (several months to a year is
routine). The AIC FIB-SEM unit will be the 9th system of a large fleet of FIB-SEMs at Janelia.

More details on the FIB-SEM position can be found here: https://tinyurl.com/y998bx5p

The AIC is rapidly expanding its scope and its portfolio, we are looking for more highly motivated
talents to join our exciting team! This position may eventually also be involved in several advanced
correlative imaging techniques currently being developed at Janelia.


Regards,
Leong

---
Teng-Leong Chew
Director, Advanced Imaging Center
HHMI Janelia Research Campus
19700 Helix Drive
Ashburn, VA 20147



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From: hsia627-at-hotmail.com
Date: Tue, 24 Apr 2018 12:39:35 -0500
Subject: [Microscopy] Image analysis workshop at U Maryland Baltimore on May 24 and 25

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I am pleased to announce the 2018 annual current EM Techniques workshop - Bio Imaging analysis will be held on May 24 and 25, 2018, at University of Maryland Baltimore. The program includes presentations on 2-D and 3-D image analysis using both open source and commercial software.

Applications that will be discussed/demonstrated include photoshop, ImageJ, Slicer, IMOD, MIPAR, Amira-Avizo, Imaris, Arivis, Dragonfly/ORS, and ImagePro. We are very excited that we will have onsite many image analysis experts and software developers to demonstrate common workflows for image analysis of biological specimens.

Demo software is available for download. Please visit the web site below for more information.

Website: http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/current-em-techniques-workshop-image-analysis/

Registration: https://umbbioimageanalysis.eventbrite.com/

Inquiry: coreimaging-at-umaryland.edu

Hope to see you in May.

---
Sincerely,
Ru-ching Hsia
rhsia-at-umaryland.edu
Associate Professor and Director
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201




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12, 61 -- CC: Ru-ching Hsia {rhsia-at-umaryland.edu}
12, 61 -- Subject: Image analysis workshop at U Maryland Baltimore on May 24 and 25
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From: Aya.Takase-at-rigaku.com
Date: Tue, 24 Apr 2018 17:06:03 -0500
Subject: [Microscopy] USC-Rigaku X-ray Microscopy Seminar

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

The University of Southern California, in conjunction with Rigaku, would like to extend an invitation for you to join us at a seminar and workshop on X-ray microscopy. This USC-Rigaku event will take place on Thursday, May 24, 2018 in Harkness Auditorium on USC's beautiful campus.

In this seminar, you will learn the latest advances in X-ray microscopy from the top level innovators in the field. You can also participate in the workshop to learn the basics of X-ray microscopy and have hands-on lab experience.

Registration: https://www.rigaku.com/mailers/2018/usc/

Contact: Michelle Goodwin michelle.goodwin-at-rigaku.com

We hope to see you there.

Sincerely,
Aya Takase


Aya Takase | Senior Scientist | Rigaku Americas Corporation
9009 New Trails Dr, The Woodlands, TX 77381 | P: 281-362-2300
www.rigaku.com





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From: wpchan-at-uw.edu
Date: Tue, 24 Apr 2018 22:06:37 -0500
Subject: [Microscopy] Re: HT on a Philips CM100

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Thank you all for the helpful tips. Here is the follow up.

1. All the power supplies in the electrical closet are on except A7
(24V h.t.) and A8 (24V fil.). When I pushed the HT on/off button, A8
came on and the red LED of A9-X2 was on. Seems like it indicates a
problem with the HT oscillator. After about 1 minute, both went off
and HT cannot be activated.

2. S34 is closed during operation. However, it does not disengage when
I lifted the gun. This could be a problem but I don't think it is
related to not being able to turn on the HT.

3. S33 is also closed during operation and it is open when the gun is lifted.

4. The 4 fuses behind the Right Hand Panel are intact. They are labelled Z201-4.

5. Upon restart, the filament type defaults to LaB6. This should not
prevent activating the HT or the filament, even if a tungsten filament
is installed.

6. Wehnelt protection circuit is not triggered. There are no red LED
lighted up for any parts of A9, except for X2 mentioned above.

7. Restarting the TEM does not help in turning on the HT.

8. On our CM100, HT defaults to 40kV upon startup. I tried various
values of kV but still couldn't turn on the HT.


--
Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519)
The Biology Imaging Facility (http://depts.washington.edu/if/)

On Thu, Apr 19, 2018 at 10:21 PM, {wpchan-at-uw.edu} wrote:
}
} Can I please get your advice on a problem with the HT on a Philips CM100?
}
} When I press the HT On/Off button to turn on the high tension, I got no
} response, i.e., I cannot turn on the high tension. The vacuum are good,
} both HiV and UHV lights are on. I pulled the power supply (A7) and found
} that the fuses are intact. The voltage going into A7 is 227V. I also
} measured the resistance across the HT On/Off push button. It is 12 ohm when
} pressed and 0 ohm released.
}
} Is there anything obvious that I have missed? Thanks!

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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Apr 2018 07:19:15 -0500
Subject: [Microscopy] viaWWW:Photo editing and annotation software results

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-------- Forwarded Message --------

X-from: holpc-at-firstenergycorp.com

This Question/Comment was submitted to the Microscopy Listserver
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Email: holpc-at-firstenergycorp.com Name: Chris Holp

Organization: FirstEnergy BETA Labs

Title-Subject: [Filtered] Photo editing and annotation software results

Message: A few weeks ago I posted a question to everyone, asking about photo editing software with
an emphasis on vector graphics for annotations.
Because there was interest expressed in the responses, at this time I want to summarize the replies
that I received:

Adobe Illustrator was recommended by one member, and Inkscape was recommended by one member.



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From: Aleksandr.Mironov-at-manchester.ac.uk
Date: Wed, 25 Apr 2018 08:45:57 -0500
Subject: [Microscopy] TEM help in recognizing biological features

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I have three questions about some features that I found in different
biologic samples. All samples were prepared in similar way with
karnovsky fixation, reduced osmium tetroxide, uranyl acetate and
standard epon embedding, no post-staining was performed. All images are
located on Google Drive (I am providing direct links).

1) Virus particles?
I have found some round membrane bound structure of 70nm diameter in two
independent samples (from different geographical places 1000 km apart):
mouse hybridoma cell line (in endoplasmic reticulum) and mouse brain
(around capillaries). These vesicles looks like some virus and this
virus seems to have the same structure in both samples. Is a viral
infection so common in mice and mouse cell lines? And what kind of virus
could it be?

Mouse hybridoma cells:

https://drive.google.com/open?id=1odDy_JBhGEL8YSP-GI_F1wfkdVd103Vl
https://drive.google.com/open?id=1AM8VIRAIpMc0Jt9mpYthJXAeZkYlqJy0

Mouse brain:

https://drive.google.com/open?id=11mf4aVppLWZe7CZjX9R22fT47mfP9dxh
https://drive.google.com/open?id=1VeT8vo4M78InzC7-0TGqI1LM5KoeKzEG

2) Mycoplasma or cell debris?
In fibroblast cell culture there are some membrane bound structure of
0.5-1 um size that are located outside cells. Is it mycoplasma infection
or some cell debris?

Links:
https://drive.google.com/open?id=1CfL-mNNFuTxwM8BoeHrc8cvblQJX7Eh8
https://drive.google.com/open?id=15uGHYJ1DgRXaR16yaszHUdpiO2QdwFCx
https://drive.google.com/open?id=14Nwo8fGz_httH_wzY4O13lt50XEIy9cA


3) Precipitate or not?
On several occasions I have seen very fine (5-6nm) and dense precipitate
in different samples. No colloidal gold was used. This precipitate tends
to concentrate in endosome-like structures and in cytoplasm. However,
nucleus, mitochondria, ER lumen ususally do not contain this kind of
precipitate. Is it just precipitate or these densely stained minuscule
granules correlate with some biological structures? I have two unrelated
samples that demonstrate similar phenomena:  endothelial cells in mouse
brain and macrophages in mouse lung. Not all cells contain this kind of
staining and this as well make me think that it may stain some real
biological structure.

Brain endothelial cells:
https://drive.google.com/open?id=15IhTI9tr1Eo0z0YLaphIX1e_dEKb5wpG
https://drive.google.com/open?id=14Z20bZPTIigFGofhyFiPsV1--hqHHa9C

Lung macrophages:
https://drive.google.com/open?id=1p9V1Y55kjdEbyVQQCVKkjf-IWo5YV13L
https://drive.google.com/open?id=1_6XpdTojVcTIGPN0anYpd3hUG4a4DWFR
https://drive.google.com/open?id=1BnW-5Medwk0AG_8-sfzAG49vlhrErR-S
https://drive.google.com/open?id=1eUlnYjOxS5auaFHcuCHnKauRM6hX64tZ

I would really appreciate any thought and ideas about these cases. Thank
you in advance!

Sincerely,
Dr. Aleksandr Mironov


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From: steven.spurgeon-at-pnnl.gov
Date: Thu, 26 Apr 2018 11:04:24 -0500
Subject: [Microscopy] Stereomicroscope recommendations for TEM holder loading

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

We’re looking to purchase a new stereomicroscope for loading of TEM sample holders and I wanted to learn more about what models others are using. We thought it might be helpful to have a digital viewing screen, though we’re not opposed to using traditional binoculars.

Any suggestions? Thanks!

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com
www.pnnl.gov



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From: steven.spurgeon-at-pnnl.gov
Date: October 8-10, 2018
Subject: [Microscopy] Save-the-date for NexTEM Workshop at PNNL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

Please see below for details about an exciting upcoming meeting we are planning for October 8-10, 2018 at PNNL. More details about the agenda and abstract submission will be sent in a couple weeks. We hope that you will be able to attend!

----------------

Next-Generation Transmission Electron Microscopy (NexTEM) Workshop
Beyond Current Limits of Resolution, Environments, and Data Analysis


Overview

Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.

Topics

High-Resolution Imaging and Spectroscopy
– New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems.
– Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision.
– Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces.
– Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples.
– Novel detectors for improved high-speed imaging and spectroscopy.

In Situ / Operando Microscopy and Extreme Environments
– Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids.
– Investigation of materials under stimulus across a range of sample environments and temperatures.

Computational Methods for Data Analysis
– Software to improve data collection quality, accuracy, and acquisition rates.
– Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis.
– Machine learning approaches for high-throughput data processing and feature detection.
– Image simulation tools to aid the interpretation of experimental images and spectroscopy.

Confirmed Invited Speakers
– Ondrej Krivanek, Nion Co.
– Amanda Petford-Long, Argonne National Laboratory
– Robert Klie, University of Illinois–Chicago
– Khalid Hattar, Sandia National Laboratories
– Renu Sharma, National Institute of Standards and Technology
– Marc DeGraef, Carnegie Mellon University
– James LeBeau, North Carolina State University
– Haimei Zheng, Lawrence-Berkeley National Laboratory
– Colin Ophus, Molecular Foundry
– Juan Carlos Idrobo, Oak Ridge National Laboratory
– R. Lee Penn, University of Minnesota
– Paolo Longo, Gatan
– Lewys Jones, Trinity College–Dublin
– Rama Vasudevan, Oak Ridge National Laboratory

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com
www.pnnl.gov



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From: microscopy.listserver-at-gmail.com
Date: Fri, 27 Apr 2018 18:07:00 -0500
Subject: [Microscopy] viaWWW:3010

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Email: dcristofori-at-unive.it Name: Davide Cristofori

Organization: Universita' Ca' Foscari Venezia

Title-Subject: [Filtered] TEM: cooling water in Jeol JEM-3010

Message: Dear Listers,
I'm using the on-line form for some problems with automatic attachment to my organization e-mails.

I'm looking for some advice about the cooling water flows of the JEOL JEM-3010 in our lab.

The cooling water flow of the the GATAN Model 794 CCD camera has fallen to 8 l/h, below the minimun
of 9 l/h required in the manual. This water line is in parallel with the one cooling the diffusion
pump of the microscope, and I can recover the right value for the CCD water flow by readjusting
pressure (from 250 to 260 kPa) and flowrate (from 160 to 170 l/h) of the DP line . Now, the doubt
is that the DP water flow is currently set at a value higher than the maximun one required, which I
believe is about 120 l/h.

Otherwise, I should try to replace the cooling water line of the CCD camera - maybe it is just dirty
- but I don't feel very comfortable in taking away the pipes directly from the body of the CCD
camera: I'm afraid that its alignment could be lost.

Any advice will be greatly appreciated.
Thanks in advance.

Davide


​~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
Davide Cristofori

direct phone = 041.234.67.26
e-mail = dcristofori[at]unive[dot]it

​Centre for Electron Microscopy "Giovanni Stevanato" ​and
​Department of Molecular Sciences and Nanosystems
Ca' Foscari ​UNiversity of Venice

Campus Scientifico, Edificio ETA
Via Torino 155 I-30172 Mestre (VE) Italy
~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~


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From: microscopy.listserver-at-gmail.com
Date: Sat, 28 Apr 2018 16:27:33 -0500
Subject: [Microscopy] =?UTF-8?Q?Fwd:_=e2=80=9cDiaspro_Lab_-_Nanoscale_Microscopy_School_i?=

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Beavers, Roy {rbeavers-at-mail.smu.edu}





-------- Forwarded Message --------


X-from: Alberto Diaspro {sekkio-at-mac.com}
——
Dear friends,

Diaspro Lab - https://www.iit.it/index.php/people/alberto-diaspro - offers a great Nanoscale School
at the Venetian Institute of of Sciences (IVSLA), Letters and Arts. All info at
https://mix.iit.it/events/ivsla-2018#link_tabfor program, registration and updates and a limited
number of seats.

IVSLA International School on Nanoscale Optical Microscopy
Directors: Alberto Diaspro (IIT, UNIGE), Paolo Bianchini (IIT), Giorgio Giacometti (IVSLA).

12 - 15 June 2018, Venice, Italy

The objective of this School is to advance the field of nanoscale optical microscopy operating at
the scale of nanometres to tens of nanometres, through the exchange of information, ideas, and
innovative techniques. The understanding of methods and techniques has the great potential of
allowing, in the near future, for the design and performance of new exciting experiments in
Biophysics, Optics and Photonics. Shots of current technologies by companies leaders in the field.

An amazing blend of top level lecturers ready for lessons, seminars and student interactions in the
amazing scenario offered by Venice in June.

Confirmed Faculty: • Francesca Romana Bertani • Paolo Bianchini • Gertrude Bunt • Loredana Casalis •
Giberto Chirico • Nader Cristelle • Alessandro Esposito • Pekka Hänninen • Mike Heilemann • Luca
Lanzanò • Aymeric Le Gratiet • Davide Mazza • Ammasi Periasamy • Alexander Rorbach • Colin Sheppard
• Peter So • George Stanciu • Peter Saggau • Peter Török • Alessandro Tredicucci • Fred Wouters •
Alberto Diaspro.

Lessons will be held in the magnificent Palazzo Loredan, Venice - https://youtu.be/fmuk-wKMuEI.

Due to high number of requests we added more seats. No deadline: first in first out acceptance
criteria. Special fees for IIT/UNIGE, SIOF and SIBPA members.

See you in a fabulous Venice in June.
All the best
Alby

(logistics: Mrs.Manuela Salvatori, subject: IVSLA2018 logistics, manuela.salvatori-at-iit.it

-----

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From: microscopy.listserver-at-gmail.com
Date: Mon, 30 Apr 2018 17:50:23 -0500
Subject: [Microscopy] viaWWW:Cryosectioning/Immunogold Workshop

Contents Retrieved from Microscopy Listserver Archives
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David-Couple of tricks here: first, check the filter in your chiller. If it is torn, you can get some crud passed to the microscope which then gets lodged in the camera before it causes a problem with the microscope. The first step to dislodge crud from the cooling lines is disconnect the upstream quick-disconnect connector, and reconnect it multiple times. The idea is to get air in the line so that bubbles form. Hopefully you have transparent lines and can watch the bubbles pass through. If that does not work, you will need to shut off the camera power, shut off the water supply, remove the quick-disconnect connectors and blow out the lines. After re-assembly, check the camera temperature using the software.

A. John Mardinly, Ph.D., P.E.


} On Apr 27, 2018, at 4:25 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: dcristofori-at-unive.it Name: Davide Cristofori
}
} Organization: Universita' Ca' Foscari Venezia
}
} Title-Subject: [Filtered] TEM: cooling water in Jeol JEM-3010
}
} Message: Dear Listers,
} I'm using the on-line form for some problems with automatic attachment to my organization e-mails.
}
} I'm looking for some advice about the cooling water flows of the JEOL JEM-3010 in our lab.
}
} The cooling water flow of the the GATAN Model 794 CCD camera has fallen to 8 l/h, below the minimun
} of 9 l/h required in the manual. This water line is in parallel with the one cooling the diffusion
} pump of the microscope, and I can recover the right value for the CCD water flow by readjusting
} pressure (from 250 to 260 kPa) and flowrate (from 160 to 170 l/h) of the DP line . Now, the doubt
} is that the DP water flow is currently set at a value higher than the maximun one required, which I
} believe is about 120 l/h.
}
} Otherwise, I should try to replace the cooling water line of the CCD camera - maybe it is just dirty
} - but I don't feel very comfortable in taking away the pipes directly from the body of the CCD
} camera: I'm afraid that its alignment could be lost.
}
} Any advice will be greatly appreciated.
} Thanks in advance.
}
} Davide
}
}
} ​~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~~~~~~~
} Davide Cristofori
}
} direct phone = 041.234.67.26
} e-mail = dcristofori[at]unive[dot]it
}
} ​Centre for Electron Microscopy "Giovanni Stevanato" ​and
} ​Department of Molecular Sciences and Nanosystems
} Ca' Foscari ​UNiversity of Venice
}
} Campus Scientifico, Edificio ETA
} Via Torino 155 I-30172 Mestre (VE) Italy
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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences Microscopy Academy

Title-Subject: [Filtered] Cryosectioning/Immunogold Workshop

Message: EMS Microscopy Academy announces our Cryosectioning/Immunogold Workshop. Five days of
hands-on training for students, researchers, and microscopists who want to learn the most up to date
theory and practice in cryosectioning and immunogold labeling.

Led by experts in their fields, Helmut Gnaegi (DiATOME) and Peter van de Plas (Aurion), this class
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June 4 - 8, 2018
Monday - Friday
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From: microscopy.listserver-at-gmail.com
Date: Mon, 30 Apr 2018 17:51:28 -0500
Subject: [Microscopy] viaWWW: Razor blades for block trimming

Contents Retrieved from Microscopy Listserver Archives
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Email: lavoie-at-uw.edu Name: Ellen Lavoie

Organization: University of Washington

Title-Subject: [Filtered] Razor blades for block trimming

Message: It seems as though the Solingen carbon steel razor blades are not available anywhere in the
USA anymore. Does anyone have a secret source? I found them on a site in Germany but the cost is
twice as much (and that's not including shipping!) that I've paid in the past. I've tried half a
dozen and none come even close to being as good and/or long lasting for trimming blocks for microtoming.

Thoughts, experiences with other blades that are just as good?
Cheers,
Ellen Lavoie UW Molecular Analysis Facility

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16, 53 -- Subject: viaWWW: Razor blades for block trimming
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From: microscopy.listserver-at-gmail.com
Date: Tue, 1 May 2018 19:35:44 -0500
Subject: [Microscopy] viaWWW:Philadelphia Society for Microscopy,SPRING SYMPOSIUM MAY 16

Contents Retrieved from Microscopy Listserver Archives
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Email: beverly.e.maleeff-at-gsk.com Name: Bev Maleeff

Organization: Philadelphia Society for Microscopy

Title-Subject: [Filtered] Philadelphia Society for Microscopy Spring Symposium

Message: Philadelphia Society for Microscopy
SPRING SYMPOSIUM MAY 16 8:00 AM - 4:00 PM
at the
SINGH CENTER, UNIVERSITY OF PENNSYLVANIA

RESEARCH AT THE LEADING EDGE OF ELECTRON MICROSCOPY

SPEAKERS:

David C. Martin, University of Delaware
In-Situ Imaging of Polymer and Organic Molecular Materials by Transmission Electron Microscopy

Bojeong Kim, Temple University
Application of Analytical Transmission Electron Microscopy Techniques for the Discovery and
Characterization of Nano-sized Environmental Samples

Masashi Watanabe, Lehigh University: MAS Tour Speaker, Keynote:
Atomic-level Imaging and Analysis of Materials by Aberration-Corrected Scanning Transmission
Electron Microscopy

Vera Moiseenkova-Bell, University of Pennsylvania
Cryo Electron Microscopy at the Forefront of Molecular Medicine and Drug Discovery

Eric A. Stach, University of Pennsylvania
Advanced Electron Microscopy of Materials at the Singh Nanotechnology Center

VENDOR EXHIBITS INCLUDING: Electron Microscopy Sciences, ThermoFisher Scientific (FEI), Structure
Probe Inc., Gatan, I.Miller, IXRF and ThermoNoran
AND -- A Tour of the Singh Nanotechnology Center

Registration, Schedule and Details:
http://phillyscope.com/

Online Registration:
$25-Regular Member / $10-Student
FREE for Students who bring a poster to present.
LUNCH INCLUDED with online Registration!


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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 May 2018 19:34:41 -0500
Subject: [Microscopy] viaWWW:Post-doc Position in In-Situ TEM/STEM available at the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Basgall,Edward {ejb63-at-drexel.edu}
try silly putty or mounting


http://www.crayola.com/faq/another-topic/what-are-the-ingredients-of-silly-putty/

{http://www.crayola.com/faq/another-topic/what-are-the-ingredients-of-silly-putty/}

What are the ingredients of Silly Putty FAQ | crayola.com
{http://www.crayola.com/faq/another-topic/what-are-the-ingredients-of-silly-putty/}
www.crayola.com
Silly Putty is made primarily from silicone and color pigments. Silly Putty was discovered in 1943
by James Wright and introduced to the public in 1950 by Peter Hodgson. Crayola acquired the
exclusive manufacturing rights to Silly Putty in 1977. The formulas are considered proprietary ...

or from WalMart

Duck Mounting Putty, Removable, 56G, 12PK/CT, BE - DUCPTY2CT Mounting Putty is ideal for temporary
mounting of paper items such as posters, charts and decorations to nearly any surface without damage
to paper item or surface you are mounting it on. It provides a safe alternative to tape, nails,
glues, tacks and staples. Mounting putty is nontoxic and easily removable and reusable. Conforms to
ASTM-4236.


best


cheers

ed


Edward J Basgall, PhD

Microscopy Technician

Physical address: Materials Characterization Core

106 Bossone Research Enterprise Center

3120 Market St., Philadelphia PA


Mailing address: Drexel University

3141 Chestnut St

MC 27-344

Philadelphia, PA 19104


Office: (215) 895-2379 FAX (215) 895- 6760

----------------------------------------------------------------------------------------------------
*From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
*Sent:* Friday, April 27, 2018 7:30:57 PM
*To:* Basgall,Edward
*Subject:* [Microscopy] Fwd: Non Crystalline Clay or Putty



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X-from: Beavers, Roy {rbeavers-at-mail.smu.edu}





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Email: rfklie-at-uic.edu Name: Robert Klie

Organization: University of Illinois

Title-Subject: [Filtered] Post-doc Position in In-Situ TEM/STEM available at the University of
Illinois - Chicago

Message: The University of Illinois at Chicago (UIC) invites applications to fill a Postdoctoral
Research Associate position in the Department of Physics under the supervision of Professor Robert
F. Klie. This position will be part of the Joint Center for Energy Storage Research (JCESR) at
Argonne National Laboratory and focus on in-situ characterization of rechargeable battery cathode
materials using high-resolution scanning transmission electron microscopy and spectroscopy. The goal
of this research program is to correlate electro-chemical measurements with structural changes of
the cathodes at the nano-scale. Close collaboration with JCESR scientists and access to the ANL
facilities will be possible as part of this project.

Please use the following link to apply for this position:
https://jobs.uic.edu/job-board/job-details?jobID=95602


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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 May 2018 19:35:30 -0500
Subject: [Microscopy] viaWWW: Engineer XRD - SEM Position at the Research Service Centers

Contents Retrieved from Microscopy Listserver Archives
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Email: ademp-at-eng.ufl.edu Name: Luisa Amelia Dempere

Organization: University of Florida

Title-Subject: [Filtered] Engineer XRD - SEM Position at the Research Service Centers

Message: Classification Title: Engineer II
Job Description:
-Operation of the instrumentation in the facility to include: 1) X-ray diffraction instrumentation
2) Scanning Electron Microscopy 3) Energy Dispersive Spectroscopy 4) Raman Spectroscopy 5) Fourier
Transformed Infrared analysis as indicated below:
-Operate the equipment for service to faculty, students and external users
-Train faculty and students on the use of the equipment and instrument’s software for data reduction
and analysis
-Assist with the laboratory practices for formal and short courses on X-ray diffraction
-Develop operating procedures and use protocols for the facilities under his/her supervision.
-Maintain the equipment, install new instrumentation and software, monitor computer records of
equipment use, and supervise scheduling of the equipment use.
-Other job duties as assigned to support the RSC.
Advertised Salary:
$50,000 - $55,000 commensurate with qualifications and experience
Minimum Requirements:
Bachelor's degree in an appropriate area and two years of relevant experience; or a high school
diploma or equivalent and six years of relevant experience. Appropriate college coursework or
vocational/technical training may substitute at an equivalent rate for the required experience.
Preferred Qualifications:
Master’s degree in appropriate area of specialization
Prefer advanced degree in physics, chemistry, materials science, electrical engineering or chemical
engineering.
Knowledge of materials characterization and analysis techniques
Experience with vacuum technology and gas handling systems
Experience with software for data reduction and analysis
Experience with X-ray diffraction instrumentation
Experience with Scanning Electron Microscopy Experience with Energy Dispersive Spectroscopy
Experience with Raman Spectroscopy
Experience with Fourier Transformed Infrared analysis Ability to work independently
Knowledge of laboratory safety practices
Ability to establish and maintain effective working relationships with others
Ability to communicate clearly both verbally and in writing
Ability to maintain records and create reports
Ability to interact cordially with coworkers to accomplish common tasks.

Special Instructions to Applicants: Please apply at:
http://explore.jobs.ufl.edu/cw/en-us/job/506687/engineer-ii

In order to be considered, applicants must upload a cover letter and resume.
Application must be submitted by 11:55 p.m. (ET) of the posting end date.
This requisition has been reposted. Previous applicants do not need to reapply.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 May 2018 06:57:32 -0500
Subject: [Microscopy] Fwd: Differential interference contrast microscope to image thin

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-------- Forwarded Message --------

X-from: YY YY {rongchigram79-at-yahoo.com.sg}



Dear All, I would like to ask for your advice, if it is 'good to have' or a 'must' to equip a light
microscope with the differential interference (DIC) contrast kit to view the thin sections from
ultramicrotomy? What are the benefits/information can we gain when we use the DIC to look at the
thin sections before we use the TEM to view them?

Lastly, do we need both reflectance and transmittance DIC to view the thin sections or either one of
them? I ask this because we are running a tight budget to get a new light microscope.

Cheers,
Yee Yan

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 May 2018 06:58:21 -0500
Subject: [Microscopy] Fwd: Open position at IST Austria: Cryo-EM Specialist (f/m)

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Ludek LOVICAR {ludek.lovicar-at-ist.ac.at}



Cryo-EM Specialist (f/m)

FULL TIME (40h)

IST Austria is a constantly growing international institute for conducting frontier research in the
life, physical, and formal sciences, located in Klosterneuburg on the outskirts of Vienna in Austria.

Electron Microscopy facility at IST Austria is expanding in the cryo-EM field. The complete cryo-EM
infrastructure, which covers both SPA and cryo-ET workflow on the top level, is going to be
established at IST Austria within this year. Following setups are going to be installed: 300kV TEM
Titan Krios (Phase-plate, 2x DDD, BioQuantum); 200kV TEM Glacios (Phase-plate, DDD), Cryo-FIB/SEM
Aquilos. To strengthen our team, we are looking for a person fulfilling our requirements who will be
highly motivated to take over cryo-EM related responsibility in a challenging international environment.

*Responsibilities*

* Operating and maintenance of cryo-EM equipment.
* Monitoring of systems, regular system check including systems alignment.
* Training, assistance, on-site support and supervision of users.
* Monitoring of compliance with rules.
* Active contribution to the development of the EM facility.

*Requirements*

* An advanced degree (M.S./Ph.D.) in Biological Sciences, Biochemistry, Structural Biology or
Physics.
* Practical experience with cryo-EM application areas (sample preparation, single particle and/or
tomography data collection, data processing), which includes all or most of the following SW:
EPU, FEI Tomography suite, Serial EM, Imod, Relion.
* Experience with cryo-CLEM and FIB milling is an advantage.
* Min. 2 years of high-end cryo-EM hands-on experience.
* Excellent team player that can also work independently.
* High degree of reliability, organizational and interpersonal skills.
* Service oriented approach towards researches.
* Excellent communication, presentation and writing skills in English.

*To apply for this position send your application in one combined pdf (including CV, certificates
and references) by e-mail to:* recruiting-at-ist.ac.at {mailto:recruiting-at-ist.ac.at}

*Contact*

Ludek Lovicar

Manager of Electron Microscopy Facility

Phone: +43-(0)2243 9000 1066

Email: ludek.lovicar-at-ist.ac.at {mailto:ludek.lovicar-at-ist.ac.at}

Website: www.ist.ac.at {http://www.ist.ac.at}


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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 May 2018 06:59:39 -0500
Subject: [Microscopy] Fwd: Microbeam Analysis in the Earth Sciences - Bristol, UK Sept 2018

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Stuart Kearns {Stuart.Kearns-at-bristol.ac.uk}

Hi All,

EMAS (European Microbeam Analysis Society) and the Mineralogical Society of GB and Ireland are
running a workshop aimed at post-graduate students and research workers on Microbeam techniques in
the Earth Sciences.

The training workshop will run from 4th – 7th September at the University of Bristol, UK

Topics covered include SEM, EPMA, CL, EBSD, TEM, LA-ICPMS, SIMS, XANES, XRF, FIB, Raman, FTIR,
MicroCT and APT, with presentations consisting of tutorial on the technique by analytical experts
followed by application talks from renowned Earth Scientists. Also poster sessions and contributed
talks.

Student early registration is £350 including accommodation (and most meals) and there are
bursaries available to help with these costs.

Deadline for abstracts for bursary applications is 15th May.
https://www.microbeamanalysis.eu/events/event/51-emas-2018-microbeam-analysis-in-the-earth-sciences

If anybody would like a poster for the event, please e-mail me offline and I will forward a pdf.

Best wishes,
Stuart
-------------------------------------------------
Stuart Kearns
School of Earth Sciences
University of Bristol, UK
Tel. +44 (0)117 331 5004/5012
Stuart.Kearns-at-bristol.ac.uk
-------------------------------------------------



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From: stefan.diller-at-t-online.de
Date: Sun, 6 May 2018 04:14:34 -0500
Subject: [Microscopy] JEOL JSM 820 scan problems - keyboard and tools needed

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Dear All,

maybe someone can help me with scan problems on a Jeol JSM 820 SEM which got donated to a German highschool...

The scans are looking like this:

https://drive.google.com/open?id=1NNsow4B0hueBnxLHN8MhE63532qa14tM

In TV-Scan I get these black and white bars, in Slowscan1 I get gibberish which might come from the alphanumeric characters
showing up at the top of the TV-scan frame.

I do so for a short time the "normal" scan image with WD and HV values displayed at the bottom of the frame when I press the CPU
reset switch at the console.

Since the some parts of the SEM vanished during a two year storage (like keyboard and service tools) it also could be the case
that the SEM needs the attached keyboard to properly display the scans.

Vacuum and HV is working but I don`t see any change when heating up the cathode and checking for a video signal.

I took out all the PCBs and cleaned the connectors. No change...


Does anybody have an idea what might be the fault? Could an empty backup battery at the FIS board be one part of the problem?

I am also loooking for a spare keyboard. Can I use one of the JSM 840 keyboards also?

And I am looking for a set of service tools...



Thanks for helping,

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
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Websites:
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www.stefan-diller.com
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From: microscopy.listserver-at-gmail.com
Date: Mon, 7 May 2018 17:28:29 -0500
Subject: [Microscopy] viaWWW:PhD and Postdoctoral job openings at University of Antwerp -

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Date: Mon, 7 May 2018 17:29:15 -0500
Subject: [Microscopy] viaWWW: Materials Science Product Specialist position open

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From: microscopy.listserver-at-gmail.com
Date: Mon, 7 May 2018 17:29:53 -0500
Subject: [Microscopy] viaWWW:Deben BSD on JEOL SEM

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Organization: MVA Scientific Consultants

Title-Subject: [Filtered] Deben BSD on JEOL SEM

Message: Does anyone have experience with installing a Deben BSD on a JEOL SEM (any JEOL SEM)?
Looking for some feedback as we consider making a purchase.

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From: trh-at-uoregon.edu
Date: Wed, 9 May 2018 04:03:40 -0500
Subject: [Microscopy] aberrations with JEOL 2100F high-tilt pole piece

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Hi all,
We have a JEOL 2100F with a high-tilt pole piece installed (and no
aberration corrector), and we've noticed that the aberrations picked up in
the objective lens strongly depend on beam shift. Even with properly
aligned shift and tilt compensation, and with mini-condenser and objective
mini-lens turned off, a beam shift of ~20 um in either direction turns an
initially round, focused, well stigmated beam to acquire a massive coma.
This seems to happen in the objective lens. Equivalently, expanding the
beam past a certain point with the condenser system produces huge caustics
at the edges. We've noticed that this doesn't happen when we defocus the
objective lens by at least 100 um in either direction from the 'standard
focus' that we set according to the service manual, but it's then a little
harder to put the specimen in an image plane.

Has anybody else experienced this with this pole piece or a different one
on the 2100F or a similar instrument? If so, how did you fix it? Did you
just set a new 'standard focus' and align the projection/imaging system to
compensate for the defocus?
Tyler

--
Dr. Tyler Harvey
Georg-August-Universität Göttingen
IV. Physical Institute
Friedrich-Hund-Platz 1
37077 Göttingen
Germany


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From: microscopy.listserver-at-gmail.com
Date: Wed, 9 May 2018 07:17:55 -0500
Subject: [Microscopy] viaWWW: SEM rotary pump's gas ballast?

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Email: shim0102-at-umn.edu Name: Tsutomu "Shimo" Shimotori

Organization: University of Minnesota Duluth

Title-Subject: [Filtered] SEM rotary pump's gas ballast?

Message: Hello.

I was replacing mist filters for our JEOL SEM's rotary pumps recently, when
it occurred to me that I never ballasted the pumps.
In my previous job, I used a lot of mass spectrometers, and we ballasted
the rotary pumps once a week.
Mass spectrometers handle a lot of solvents, while SEMs don't.
So I'm guessing that we almost never have to ballast rotary pumps for SEM.
Am I correct?
Our SEM has Variable Pressure capability, but we don't analyze wet or moist
samples frequently.
If anybody has any thought on this subject, I would appreciate it.

Thank you.

----------------------------------------------------------
Tsutomu "Shimo" Shimotori
Research Instrumentation Laboratory manager
University of Minnesota - Duluth

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From: John.Mardinly-at-asu.edu
Date: Wed, 9 May 2018 15:42:47 -0500
Subject: [Microscopy] Re: viaWWW: SEM rotary pump's gas ballast?

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Mist filters? Are you dumping pump exhaust into interior air that people actually breath? Is this legal anywhere anymore? It’s certainly not a ‘best practice’.


A. John Mardinly, Ph.D., P.E.

} On May 9, 2018, at 5:33 AM, microscopy.listserver-at-gmail.com wrote:
}
}
}
}
}
} X-from: shim0102-at-umn.edu
}
} ---------------------------------------------------------------------------
} Remember this posting is most likely not from a Subscriber, so when replying please copy both
} shim0102-at-umn.edu as well as the Microscopy Listserver
} ---------------------------------------------------------------------------
}
} Email: shim0102-at-umn.edu Name: Tsutomu "Shimo" Shimotori
}
} Organization: University of Minnesota Duluth
}
} Title-Subject: [Filtered] SEM rotary pump's gas ballast?
}
} Message: Hello.
}
} I was replacing mist filters for our JEOL SEM's rotary pumps recently, when
} it occurred to me that I never ballasted the pumps.
} In my previous job, I used a lot of mass spectrometers, and we ballasted
} the rotary pumps once a week.
} Mass spectrometers handle a lot of solvents, while SEMs don't.
} So I'm guessing that we almost never have to ballast rotary pumps for SEM.
} Am I correct?
} Our SEM has Variable Pressure capability, but we don't analyze wet or moist
} samples frequently.
} If anybody has any thought on this subject, I would appreciate it.
}
} Thank you.
}
} ----------------------------------------------------------
} Tsutomu "Shimo" Shimotori
} Research Instrumentation Laboratory manager
} University of Minnesota - Duluth
}
} Login Host: 131.212.168.48
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}



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From: microscopy.listserver-at-gmail.com
Date: Wed, 9 May 2018 20:39:39 -0500
Subject: [Microscopy] viaWWW: SEM rotary pump's gas ballast?

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-------- Forwarded Message --------

X-from: James M. Ehrman {jehrman-at-mta.ca}


Hello,

We always ballasted TEM rotary pumps once a week, back in the day when
they used film. Wouldn't seem to be necessary with an SEM, unless you
use LV mode a lot with dirty specimens. I'm not sure that it would hurt,
but in changing RP oil in our SEM once a year, I don't see any problems
with the used oil coming out of the pump. We use LV mode occasionally as
well, but common sense in terms of sample size and amount of outgassing
materials shouldn't really make this an issue.

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: http://www.mta.ca/dmf

The 6 Stages of a Project:

1 Wild Enthusiasm
2 Total Disillusionment
3 Utter Panic
4 Search for the guilty
5 Punishment of the innocent
6 Promotion of the non-participants


On 5/9/2018 9:18 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: shim0102-at-umn.edu Name: Tsutomu "Shimo" Shimotori
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} Organization: University of Minnesota Duluth
}
} Title-Subject: [Filtered] SEM rotary pump's gas ballast?
}
} Message: Hello.
}
} I was replacing mist filters for our JEOL SEM's rotary pumps recently, when
} it occurred to me that I never ballasted the pumps.
} In my previous job, I used a lot of mass spectrometers, and we ballasted
} the rotary pumps once a week.
} Mass spectrometers handle a lot of solvents, while SEMs don't.
} So I'm guessing that we almost never have to ballast rotary pumps for SEM.
} Am I correct?
} Our SEM has Variable Pressure capability, but we don't analyze wet or moist
} samples frequently.
} If anybody has any thought on this subject, I would appreciate it.
}
} Thank you.
}
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From: baskin-at-bio.umass.edu
Date: Thu, 10 May 2018 08:46:19 -0500
Subject: [Microscopy] Ask a Microscopist - microscopic effects on

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***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely
not a member the listserver, and
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Please copy their email address from their question.
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Name: Beth Dixon
Grade/Education Level: High School
Location:Rocklin, CA US
Email: bdixon-at-rafos.org


Hi Beth
            This sounds like fun. You can cut hand sections with a
razor blade and look at them in brightfield (or fluoresence if you have
that ability). Many plant tissues are auto-fluorescent. If you have
access to a drawer with stains, you can try staining. Lots of these dyes
stain plant tissues differentially and this can simply provide more
contrast for you (in either brightfield or fluoresence). It doesn't
matter really what exactly they stain (and in many cases that won't be
well known).

    I am a little curious about the control your student used? Plants
do have steriod-type hormones but they are not exactly like those of
animals. And certainly some steriod hormones don't do anything to
plants. I wonder if there are other materials in the inhaler 'juice' ? I
would be happy to correspond with you off-line about that if you like.

    Good luck!
                    Tobias

On 5/10/18 8:09 AM, oshel1pe-at-cmich.edu wrote:
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}
} Name: Beth Dixon
} Grade/Education Level: High School
} Location:Rocklin, CA US
} Email: bdixon-at-rafos.org
}
} Subject: Plant Tissue and Steroid Light Microscopy Ideas
} Your Question:
} Hello Microscopists! I am a high school biology teacher with a student question. My student has asthma, and her mother, a nurse, had a drawer full of expired inhalers that they wanted to put to use for her 9th grade science project. Using a syringe, they injected plant seedlings with the steroid and monitored their growth. The growth rate of the treated plants was obviously much higher than the non-treated plants, but the student wants to extend her project to see if there are any microscopic differences that she could visualize at the tissue level of the plants using a light microscope. Aside from building stomatal peels, I don't have much experience with plant microscopy, especially not when it comes to hormone detection. Does anyone know of
}
}
}
} something she could look for?
} Thanks! Beth
}
}
}
}
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--
__ ___ ^ ___ ___ Tobias I. Baskin
/ \ / / \ / \ Professor
/ / / / \ \ \ Biology Department
/ __/ /__ /___ \ \ \__ University of Mass.
/ / / \ \ \ 611 N. Pleasant St.
/ / / \ \ \ Amherst, Massachusetts
/ /___ / \ \___/ \_____ USA 01003 413-545-1533
bio.umass.edu/biology/baskin BLOG: blogs.umass.edu/baskin/


==============================Original Headers==============================
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From: sergei2-at-ornl.gov
Date: Fri, 11 May 2018 14:57:54 -0500
Subject: [Microscopy] Historical references - direct electron beam effects on structure of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues - I am looking for advice and historical references (say
prior to 2000) on direct electron beam matter patterning, including
beam-induced atom and particle motion, chemical changes induced by
electron beam and detected locally via direct atomic imaging or
diffraction, beam induced chemical reactions, sculpting, etc. Our group
now actively works on harnessing these phenomena for nano- and atomic
scale fabrication, and we are extremely interested in past work in these
areas. My experience was that many such observations were reported in a
very broad spectrum of journals ranging from EM to surface science and
applied physics, and very often key words do not allow for effective ISI
searches - so I would greatly appreciate pointers to groups and specific
papers along those lines.

Thank you in advance!

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, Foresight Institute, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Sat, 12 May 2018 06:56:57 -0500
Subject: [Microscopy] viaWWW:What LIMS are you using for microscopy ?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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X-from: alexandre.bastien-at-fsaa.ulaval.ca

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Email: alexandre.bastien-at-fsaa.ulaval.ca Name: Alexandre Bastien

Organization: Universit Laval

Title-Subject: [Filtered] What LIMS are you using for microscopy ?

Message: Hi all,

I'm looking for a laboratory information management system (LIMS) for microscopy. I don't need all
the online analysis provided by OMERO, and I'm looking for something simpler. Many of our students
just save their data on our server with stupid names like test123. I'm looking for a way to stick a
protocol next to every image taken in a clean searchable way. For example, if the image is
immunofluo of a bovine embryo, I would want the user to select from a list of protocols, then enter
specie, cell type, antibody, wavelength, etc. The actual microscope settings are not so important
because they are already stored in the metadata. The best solution would be something open source
that we could run locally on our servers.

Many thanks!

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From: microscopy.listserver-at-gmail.com
Date: Sat, 12 May 2018 06:57:59 -0500
Subject: [Microscopy] viaWWW:Available: PhD scholarship at UOW, Australia

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

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Email: azdiar-at-uow.edu.au Name: Azdiar Gazder

Organization: University of Wollongong

Title-Subject: [Filtered] Available: PhD scholarship at UOW, Australia

Message: A PhD scholarship on the deformation behaviour of a metastable titanium alloy is available
at the University of Wollongong, Australia.

For further information, please click on the following weblink:
https://eis.uow.edu.au/content/groups/public/-at-web/-at-eis/documents/doc/uow247105.pdf


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From: microscopy.listserver-at-gmail.com
Date: Sat, 12 May 2018 06:58:34 -0500
Subject: [Microscopy] viaWWW:Search for MCCB electronic board for FEI XL30 SEM microscope

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Email: yanina.fasano-at-gmail.com Name: Yanina Fasano

Organization: Low Temperature Lab, Centro Atmico Bariloche, Argentina

Title-Subject: [Filtered] Search for MCCB electronic board for FEI XL30 SEM microscope

Message: We are looking for a working MCCB/DBTR electronic board of a FEI XL30\TMP scanning electron
microscope (SEM) with motorized stage for our SEM microscope. The non-working board that we have has
the labels V5.61 and V2.10 in the board integrated circuits.
We kindly appreciate to have news from old microscopes that might have this spare part. This will
allow our microscope at Bariloche,Patagonia, Argentina, to be functional again.

Many thanks in advance,

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From: microscopy.listserver-at-gmail.com
Date: Sat, 12 May 2018 06:59:49 -0500
Subject: [Microscopy] viaWWW: Job Opening : Senior Research Associate/Bio-EM Senior

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

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Email: marilee-at-usc.edu Name: Marilee Reynolds

Organization: University of Southern California
Title-Subject: [Filtered] Job Opening at USC Viterbi School of Engineering and Dornsife College of
Letters, Arts and Sciences:Senior Research Associate/Bio-EM Senior Scientist

Message: Bio-EM Senior Scientist; Core Center of Excellence in Nano Imaging (CNI)

The USC Viterbi School of Engineering and the USC Dornsife College of Letters, Arts and Sciences is
seeking an experienced Bio-Electron Microscopy (EM) Senior Scientist to perform collaborative
research and training in a shared instrumentation facility (CNI). This core facility, comprised of
electron microscopes and associated sample preparation equipment, serves users from physical and
life sciences and engineering (www.usc.edu/dept/CEMMA). The position reports to the Directors of
the facility.

The Bio-EM Senior Scientist will collaborate with USC user groups to apply advanced EM methods to
address research problems on supported research projects. Process biological specimens for TEM and
SEM. Develops protocols for performing advanced EM techniques, including training procedures and
licensed user tests.
Qualifications: PhD in science or engineering field with at least three years of experience beyond
the PhD, demonstrable experience in TEM and SEM techniques, strong EM experience spanning sample
preparation to advanced techniques, including FIB, image filtering, EELS, spectroscopic imaging,
tomographic imaging, and STEM microanalysis.

Follow this link to the full on-line job announcement.
https://usccareers.usc.edu/job/los-angeles/research-associate-senior/1209/7928019

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From: microscopy.listserver-at-gmail.com
Date: Sat, 12 May 2018 09:09:44 -0500
Subject: [Microscopy] Fwd: Gatan OneView camera on FEI Tecnai Osiris

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-------- Forwarded Message --------

X-from: Xingzhong Li {xzli-at-unl.edu}


Dear all,

We are planning to replace the Orius camera with OneView camera on FEI Osiris.
The Osiris PC is Window XP. Operation of Orius camera is integrated in the Osiris PC. The STEM
imaging /EELS mapping is carried out using TIA (TEM imaging and Analysis) now.
Since OneView camera cannot be controlled/operated on the Osiris PC (Windows XP), a separate PC
with Window 7 or up will be used for the OneView camera. In this case, Is there a way to use TIA
with the OneView camera ? Is it possible to install the TIA software on the PC for OneView ?

STEM imaging / EELS mapping should be OK on the FEI Tecnai Osiris with the OneView camera of.

Please send your experiences and suggestions to xzli-at-unl.edu.

Thanks !
X.Z. "Jim" Li


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From: hsia627-at-hotmail.com
Date: Tue, 15 May 2018 00:53:10 -0500
Subject: [Microscopy] UMB Bio Image Analysis Workshop

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Dear Colleagues,

This is to remind you that registration for the BioImage Analysis Workshop hosted by the University of Maryland Baltimore (UMB) Electron Microscopy Core Imaging Facility (EMCIF) on May 24th and 25th will close this Friday, May 18th. Fifteen image analysis experts and software developers will be onsite to demonstrate common workflows for image analysis of biological specimens. The applications that will be discussed during the workshop include Photoshop, ImageJ, Slicer, IMOD, MIPAR, Amira-Avizo, Aivia, Imaris, Arivis, Dragonfly/ORS, and ImagePro and Zen. In most cases, demo software will be available for download.
Furthermore, there will be two instrument demonstrations featuring the newly developed 20 Megapixel CMOS TEM camera NanoSprint by AMT, and the robotic automated specimen processor ASP1000 by Microscopy Innovations.
Thanks to generous donations by our sponsors, we are able to offer 50 free registrations to graduate student and postdocs. There are still a few spaces left.
Please visit the web site below for more information.
Website: http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/current-em-techniques-workshop-image-analysis/
Registration: https://umbbioimageanalysis.eventbrite.com/
Inquiries: coreimaging-at-umaryland.edu
I look forward to seeing you at the workshop.
Sincerely,
Ru-ching Hsia
rhsia-at-umaryland.edu
Associate Professor and Director
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201



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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 May 2018 13:38:32 -0500
Subject: [Microscopy] viaWWW:HPM100 services

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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] HPM100 services

Message: Hello,

I wonder if there are any 3rd party companies/persons who are providing
services on the high-pressure freezer, HPM100.

Any information would be appreciated.

Thank you,

Naomi Kamasawa
Head, Electron Microscopy Facility
Max Planck Florida Institute for Neuroscience
One Max Planck Way
Jupiter, FL 33458 USA


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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 May 2018 16:57:23 -0500
Subject: [Microscopy] viaWWW:Available: PhD scholarship at UOW, Australia

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Email: azdiar-at-uow.edu.au Name: Azdiar Gazder

Organization: University of Wollongong

Title-Subject: [Filtered] Available: PhD scholarship at UOW, Australia

Message: A PhD scholarship on the deformation behaviour of a metastable
titanium alloy is available at the University of Wollongong, Australia.

For further information, please click on the following weblink:
https://eis.uow.edu.au/content/groups/public/-at-web/-at-eis/documents/doc/uow247105.pdf


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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 May 2018 06:31:48 -0500
Subject: [Microscopy] viaWWW: Artifact in embedded specimen

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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn

Organization: Stony Brook University

Title-Subject: [Filtered] Artifact

Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to
weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense
spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the
artifact is also present everywhere on the tissue - not on the resin area of the section-only the
tissue - mitochondria, nuclei, etc......please comment of what is causing this???
Thank you

Sue

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From: wij.muss-at-aon.at
Date: Sat, 19 May 2018 08:24:51 -0500
Subject: [Microscopy] Re: Artifact in embedded specimen

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Dear Susan,

this reminds me of "salt and pepper"-preciptates due to your fixation /
generally processing of the tissue.
NB: PB (PO4-buffer, molarity of working solution ??) and too rapid or less
careful dehydration (i.e., for example: dehydration out of buffer washes
post PFA or PFA/GA or GA with 70% EtOH (for sure 76% EtOH) may cause
(sometimes huge) PO4-precipitation within tissue.

Not knowing about your using OsO4 [if yes: in PB too?) as secondary fixative
and afterwards....(would be interesting to see your standard processing SOP
just to follow all the steps done until examination of the grids)..

Such also would happen also after processing as before + UO2ac. en bloc
tertiary fixation (= a pre-staining option) without applying rigorous
washing tissue specimens before with the maleate buffer method/sequence to
get rid of the whole phosphates.......

Worst case would be (but for sure you are able to dicriminate between
microorganisms like bacilli or bacteria from long storage PO4 or other 'ion'
precipitation in specimen in primary fix.-solutions) if there happened some
detrimental alteration of your kidney specs during storage.

Naturally it would be of benefit to see a typical micrograph/dig. image of
the precipitates (i) after conventional double staining (UO2Ac-Lead citrate)
as compared to ii) unstained ultrathin sections from same specimen.

Looking forward to further posts (would it be possible to get sent those
posts you received as only "personal replies" ?? Thank you in advance!)


To all: Happy Holidays and beautiful, 'inflaming / sparking' Pentecost /
Whitsunday,

Our /my thoughts and prayers are with the victims of the most recent rampage
-at- Santa Fe High School in Texas and their parents and relatives.
May the Lord may have mercy on them!

Respectfully, and
With my personal warm regards

Wolfgang


========================================================================
MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
FN-Tel. m.
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
inviting you to join RG
(Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html,
and join 14+ million researchers, including 63 Nobel Laureates)

Former Secretary Long standing Member (until March 2018) and
(until June2017) Board Member of the

SCUR
{The Society for Cutaneous Ultrastructure Research}
The Skin Imaging Society { www.scur.org }






Von: microscopy.listserver-at-gmail.com
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Gesendet: Samstag, 19. Mai 2018 13:56
An: wij.muss-at-aon.at
Betreff: [Microscopy] Artifact in embedded specimen

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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 May 2018 20:38:46 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Search for MCCB electronic board for FEI

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-------- Forwarded Message --------
Delivered-To: microscopy.listserver-at-gmail.com
X-from: Valery Ray {webmaster-at-partbeamsystech.com}


Hi Yanina,

The MCCB/DBTR board is fairly straight-forward to repair, but if you don't have access to decent
component lever repair service then there are coupe of MCCB boards available on E*Bay, Item number
173140010597.

Best Wishes and
Good luck!
Valery

Valery Ray
www.linkedin.com/in/valeryray/
Also with REFINE Center, UCONN
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 (leave a message)
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 5/12/2018 8:00 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: yanina.fasano-at-gmail.com Name: Yanina Fasano
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} Organization: Low Temperature Lab, Centro Atómico Bariloche, Argentina
}
} Title-Subject: [Filtered] Search for MCCB electronic board for FEI XL30 SEM microscope
}
} Message: We are looking for a working MCCB/DBTR electronic board of a FEI XL30\TMP scanning electron
} microscope (SEM) with motorized stage for our SEM microscope. The non-working board that we have has
} the labels V5.61 and V2.10 in the board integrated circuits.
} We kindly appreciate to have news from old microscopes that might have this spare part. This will
} allow our microscope at Bariloche,Patagonia, Argentina, to be functional again.
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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 May 2018 20:39:45 -0500
Subject: [Microscopy] Fwd: Gatan OneView camera on FEI Tecnai Osiris

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Stefano Rubino {stefano-at-soquelec.com}

Hi Jim,

You should ask directly FEI for an additional licence (they may decline or charge you for it), but
why do you want TIA on the OneView PC? You can acquire images with GMS 3 (Digital Micrograph) and,
as you wrote, EELS and STEM will be done with TIA on the old PC.

Cheers,
Stefano


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: May 12, 2018
7:33 AM
To: Stefano Rubino {stefano-at-soquelec.com}




-------- Forwarded Message --------

X-from: Xingzhong Li {xzli-at-unl.edu}


Dear all,

We are planning to replace the Orius camera with OneView camera on FEI Osiris.
The Osiris PC is Window XP. Operation of Orius camera is integrated in the Osiris PC. The STEM
imaging /EELS mapping is carried out using TIA (TEM imaging and Analysis) now.
Since OneView camera cannot be controlled/operated on the Osiris PC (Windows XP), a separate PC
with Window 7 or up will be used for the OneView camera. In this case, Is there a way to use TIA
with the OneView camera ? Is it possible to install the TIA software on the PC for OneView ?

STEM imaging / EELS mapping should be OK on the FEI Tecnai Osiris with the OneView camera of.

Please send your experiences and suggestions to xzli-at-unl.edu.

Thanks !
X.Z. "Jim" Li

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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 May 2018 20:40:31 -0500
Subject: [Microscopy] viaWWW:What LIMS are you using for microscopy ?

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Stefano Rubino {stefano-at-soquelec.com}


Hi,
At University of Victoria we used this one:

http://www.fomnetworks.com/about_us.html

You can also get a free trial.

Regards,
Stefano

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: May 12, 2018
5:33 AM
To: Stefano Rubino {stefano-at-soquelec.com}


------- Forwarded Message --------


X-from: alexandre.bastien-at-fsaa.ulaval.ca

This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at
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Email: alexandre.bastien-at-fsaa.ulaval.ca Name: Alexandre Bastien

Organization: Universit Laval

Title-Subject: [Filtered] What LIMS are you using for microscopy ?

Message: Hi all,

I'm looking for a laboratory information management system (LIMS) for microscopy. I don't need all
the online analysis provided by OMERO, and I'm looking for something simpler. Many of our students
just save their data on our server with stupid names like test123. I'm looking for a way to stick a
protocol next to every image taken in a clean searchable way. For example, if the image is
immunofluo of a bovine embryo, I would want the user to select from a list of protocols, then enter
specie, cell type, antibody, wavelength, etc. The actual microscope settings are not so important
because they are already stored in the metadata. The best solution would be something open source
that we could run locally on our servers.

Many thanks!

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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 May 2018 20:41:21 -0500
Subject: [External] [Microscopy] viaWWW:HPM100 services

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------
X-from: Nessler, Randy A {randy-nessler-at-uiowa.edu}


Hi Naomi,
I have had Bill service our HPF before. His contact info is:

Bill Graham
BIBST LABS
37 Averill Road
BROOKLINE, NH 03033

603-345-3887
BILL-at-BIBST.COM

Regards,
Randy

-----Original Message-----
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To: Nessler, Randy A




-------- Forwarded Message --------

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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] HPM100 services

Message: Hello,

I wonder if there are any 3rd party companies/persons who are providing
services on the high-pressure freezer, HPM100.

Any information would be appreciated.

Thank you,

Naomi Kamasawa
Head, Electron Microscopy Facility
Max Planck Florida Institute for Neuroscience
One Max Planck Way
Jupiter, FL 33458 USA


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From: microscopy.listserver-at-gmail.com
Date: Sun, 20 May 2018 12:30:39 -0500
Subject: [Microscopy] Administrivia: Important Message - This may be your Last Microscopy

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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 May 2018 08:12:04 -0500
Subject: [Microscopy] viaWWW: Artifact in embedded specimen

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-------- Forwarded Message --------

X-from: Sara E Miller, Ph.D. {mille012-at-duke.edu}



We sometimes get tissue that has been stored in formalin (not just formaldehyde) after a long
period, and while it's not as sharp as with proper fixation, it's fine, certainly diagnostic and
without precipitates. Thus, that's not your problem. It sounds like a precipitate of one solution
with another. Also, the fact that your spots are on the tissue and not in the resin point to a
pre-embedment step. Without knowing your procedure or what the dense spots look like, it would be
hard to know where in the procedure this is happening. My guess is that some unbound substance
isn't getting washed out properly. Phosphate buffers can make small precipitates in the tissues
(not on the resin), and uranyl acetate, if you're using en bloc staining, precipitates with many
buffers. Also, if at some point, the surface of the tissue was allowed to dry, that may prevent
further washes and fixatives from penetrating thoroughly.


Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Shared Resource
P. O. Box 3712
Duke Medical Center
Durham, NC 27710

Phone: 919 684-9141
Pager: 919 970-8604
Cell: 919 402-3140
Fax: 919 684-3265

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*From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
*Sent:* Saturday, May 19, 2018 7:58 AM
*To:* saram-at-duke.edu
*Subject:* [Microscopy] viaWWW: Artifact in embedded specimen



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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn

Organization: Stony Brook University

Title-Subject: [Filtered] Artifact

Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to
weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense
spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the
artifact is also present everywhere on the tissue - not on the resin area of the section-only the
tissue - mitochondria, nuclei, etc......please comment of what is causing this???
Thank you

Sue

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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 May 2018 08:12:54 -0500
Subject: [Microscopy] viaWWW: Artifact in embedded specimen

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-------- Forwarded Message --------

X-from: Townley, Debra {debrat-at-bcm.edu}

Hi Sue,

I ran into this problem several years back. Changed almost every variable that I could - water
source, filtration of buffers and fixes, new vendors for chemicals, etc. After an exhaustive search
for the culprit I found (through EDS on a new Hitachi TEM) that OSMIUM was the major offender!!! Do
you use a post-fix in OsO4? If so, you will find that adding 0.8-1% potassium ferricyanide as a
chelating agent may solve your problem. The solution will be bright yellow, like uranyl acetate; it
will still act as an oxidizer (tissue will be black at the end of an hour); you may process as
usual. It took me forever to figure it out (it seemed like forever anyway.....) and I'm hoping to
save you time and aggravation.

Cheers,
Debra M. Townley
Integrated Microscopy Core
Baylor College of Medicine

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Saturday, May
19, 2018 6:57 AM
To: Townley, Debra




-------- Forwarded Message --------

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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn

Organization: Stony Brook University

Title-Subject: [Filtered] Artifact

Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to
weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense
spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the
artifact is also present everywhere on the tissue - not on the resin area of the section-only the
tissue - mitochondria, nuclei, etc......please comment of what is causing this???
Thank you

Sue

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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 May 2018 08:14:22 -0500
Subject: [Microscopy] viaWWW: TEM: Leica UC7 vs RMC Powertome-PC

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Townley, Debra {debrat-at-bcm.edu}


Hello Joseph,

The UC7 has no place to rest your hands. Nor does it have a calibrated coarse advance wheel. There
is also a tendency for the knife holder to collect water, so if you go with the UC7, be sure to keep
that area dry overnight or else it will form rust around and over the mirror. When going from an RMC
to a Leica, it takes a little getting used to.

On the up side, the Leica has great optics, a very heavy and sturdy base and sturdy attachments.
Much less flimsy than the RMC aluminum parts.

The Boss and I argued about Leica vs RMC. I had to give up my precious RMC 6000-XL, which was my
favorite microtome ever. I was all for buying a new RMC PC, but that didn't happen. At the time the
RMC was about $20,000 cheaper than the Leica, but I see that Leica has come down on their price
these days. I really can't compare the 2 since I have not used the RMC, but I sure do like the RMC
product.

Good luck with your new tool!
Debra

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Tuesday, May
22, 2018 4:08 PM
To: Townley, Debra




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Email: joseph.mowery-at-ars.usda.gov Name: Joseph Mowery

Organization: USDA ARS ECMU

Title-Subject: [Filtered] TEM: Leica UC7 vs RMC Powertome-PC

Message: Hello,

Is there anyone who has used both the Leica UC7 and the RMC Powertome-PC, that could provide some
feedback on which is the superior ultramicrotome. The specifications seem very comparable, yet the
UC7 seems to be more popular and aesthetically much more attractive.
Is the UC7 compatible with an ATUMtome?

The Powertome has flip-up hand rests to stabilize your hands while manipulating sections, does the
UC7 have any place to rest your hands?

Any feedback would be greatly appreciated.

Best Regards
-Joe

Joe Mowery | Biologist Electron and Confocal Microscopy Unit
USDA Agricultural Research Service
Lab 301-504-9027 | Mobile 817-821-8566
joseph.mowery-at-ars.usda.gov


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From: zaluzec-at-microscopy.com
Date: Fri, 25 May 2018 18:44:13 -0500
Subject: [Microscopy] viaWWW:Hitachi H-8000 TEM DISSASSEMBLY, assistance needed

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Email: provenca-at-sccp.sc.edu Name: Aaron Provence

Organization: Private dealer/researcher

Title-Subject: [Filtered] Hitachi H-8000 TEM DISSASSEMBLY, assistance needed

Message: I am seeking someone to hire in the vicinity of Columbia, SC that has relevant experience
in working with a Hitachi H-8000 transmission electron microscope. I need assistance in
disassembling the system to prepare for its removal. Please contact me if you are experienced or
available to assist.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 May 2018 18:47:57 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Artifact in embedded specimen

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X-from: Debra Sherman {dsherman-at-purdue.edu}


I had a similar problem a few years ago. I contacted the listserve for suggestions and got the
following from Ann Ellis. She was a wonderful source of help and information. It worked for me so
give it a try.

Debby

Debra Sherman, E-mail: dsherman-at-purdue.edu www.dsimagingllc.com

=======================================================
The pepper sounds like the same old thing we have had from time to time over the years with glut and
osmium fixation. Traditional buffer washes will not solve the problem. I published a paper way
back in Stain Technology (1979) 54:282-285. We ran out of reprints twice since every pathologist and
his brother wanted one. I don't have any more or I would send you one. The salvage method is simple.
Cut new sections and pick up on nickel grids.
Oxidize the sections with 1-2% (wt/vol) freshly prepared periodic acid for 5-10 minutes. Wash the
grids several times with deionized water. [with nickel grids you can make the grids wash themselves
by setting them on a magnetic stirrer at low speed]
Post stain as usual with uranium and lead
More importantly, I do have some ideas about how to prevent the problem from happening again. In my
many years of doing cytochemical localization, I washed the tissue in buffer wash which contained
0.5-1.0% (vol/vol) DMSO. Thisa removed the aldehyde and protected the enzymatic activity. In the
last several buffer washes before localization procedures I added 0.1 M glycine to the buffer wash.
This has been recommended for years for removing unbound aldehydes to improve immunolabel. I have
never seen the osmium pepper in any of those preps.

A while back I was going back through the list server archives and Randy Tindall had a post about
putting a small amount of beta -mercaptoethanol in the buffer washes in an effort to prevent this
problem. It probably works similar to the DMSO and glycine..
Ann
On 5/25/18, 9:16 AM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:

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X-from: Sara E Miller, Ph.D. {mille012-at-duke.edu}
We sometimes get tissue that has been stored in formalin (not just formaldehyde)
after a long period, and while it's not as sharp as with proper fixation, it's fine, certainly
diagnostic and without precipitates. Thus, that's not your problem. It sounds like a
precipitate of one solution with another. Also, the fact that your spots are on the tissue and
not in the resin point to a pre-embedment step. Without knowing your procedure or what the
dense spots look like, it would be hard to know where in the procedure this is happening. My
guess is that some unbound substance isn't getting washed out properly. Phosphate buffers can
make small precipitates in the tissues (not on the resin), and uranyl acetate, if you're using
en bloc staining, precipitates with many buffers. Also, if at some point, the surface of the
tissue was allowed to dry, that may prevent further washes and fixatives from penetrating
thoroughly.
Sara E. Miller, Ph. D.
Professor, Department of Pathology
Director, Electron Microscopy Shared Resource
P. O. Box 3712
Duke Medical Center
Durham, NC 27710
Phone: 919 684-9141
Pager: 919 970-8604
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*Sent:* Saturday, May 19, 2018 7:58 AM
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*Subject:* [Microscopy] viaWWW: Artifact in embedded specimen
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Email: susan.vanhorn-at-stonybrook.edu Name: Susan Van Horn
Organization: Stony Brook University
Title-Subject: [Filtered] Artifact
Message: We are embedding kidney samples that have been stored in para/glut/PB fix for a day to
weeks--immersion fixation.......i am seeing what looks like classic lead precipitate - round dense
spheres over the tissue after staining with UA/Pb......but when we look at unstained sections the
artifact is also present everywhere on the tissue - not on the resin area of the section-only the
tissue - mitochondria, nuclei, etc......please comment of what is causing this???
Thank you
Sue
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From: rongchigram79-at-yahoo.com.sg
Date: Mon, 28 May 2018 10:19:56 -0500
Subject: [Microscopy] Servicing the Leica Ultramicrotome Ultracut UCT

Contents Retrieved from Microscopy Listserver Archives
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Dear All, may I ask if anyone has actually dismantled and service the Leica Ultramicrotome UCT Ultracut themselves? Recently I start to hear some squeaking sound from the arm when I am trimming at high speed e.g 50mm/s. I guess that the gears are running out of lubricant. However, Leica no longer supports this model and we can't get the service engineer to do the servicing. May I ask anyone has done such servicing themselves and have some tips or guidelines to share?

Best Regards,
Yee Yan, Tay
FACTS
NTU, Singapore

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From: ph2-at-sprynet.com
Date: Tue, 29 May 2018 21:35:33 -0500
Subject: [Microscopy] Inter-Micro 2018

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

FYI

Inter/Micro 2018
70th Annual International Microscopy Conference
June 11-15, 2018 McCrone Research Institute, Chicago

Info:
https://www.mccroneinstitute.org/v/101/InterMicro


Presentation List at:

https://www.mccroneinstitute.org/v/1308/2018-Speaker-Schedule




Tony

..
Andrew Anthony Tony Havics, CIH, PE
Environmental, Health & Safety, Microscopy, Materials Science & Forensic
Engineering
pH2, LLC
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From: microscopy.listserver-at-gmail.com
Date: Wed, 30 May 2018 07:25:01 -0500
Subject: [Microscopy] viaWWW:Cryo-EM facility manager at the Ernst-Ruska-Centre

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

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Email: c.sachse-at-fz-juelich.de Name: Carsten Sachse

Organization: Forschungszentrum Juelich

Title-Subject: [Filtered] Cryo-EM facility manager at the Ernst-Ruska-Centre (Forschungszentrum Juelich)

Message: Dear all,

Please find below the announcement for a cryo-EM facility manager position at the Ernst-Ruska-Centre
in Juelich (Germany).

The Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C) at the
Forschungszentrum Juelich houses some of the world's most advanced electron microscopes and tools
for nanocharacterisation. The scientific research covers current issues in condensed matter physics
and from now on cryo-EM of biomacromolecules. The ER-C has a total of 13 electron microscopes
including the PICO FEI Titan with a point resolution up to 0.5 . The facility will be extended with
state-of-the-art cryo-microscopes FEI Talos Arctica and FEI Talos 120.

We are seeking to recruit a facility manager:
http://www.fz-juelich.de/SharedDocs/Stellenangebote/_common/dna/2018-153-EN-ER-C%203.html?nn=363560

Please do not hesitate to contact me in case you have any question on the position.

Best wishes,


Carsten
____________________________________________________________________________________
Dr. Carsten Sachse
Acting Head ER-C3 Forschungszentrum Jlich GmbH
Ernst-Ruska-Centrum, Strukturbiologie
52425 Jlich
Germany
http://www.fz-juelich.de/er-c/er-c-3/EN/Home/home_node.html

Group Leader
European Molecular Biology Laboratory Meyerhofstr. 1
69117 Heidelberg
Germany
phone: + 49 6221 387 8407
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From: microscopy.listserver-at-gmail.com
Date: Fri, 8 Jun 2018 07:07:48 -0500
Subject: [Microscopy] viaWWW:Looking for a JEOL 8900

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Email: renaudgeological-at-execulink.com Name: Jim Renaud

Organization: Renaud Geological Consulting Ltd

Title-Subject: [Filtered] Looking for a JEOL 8900

Message: We are looking for a complete operating JEOL 8900 microprobe. Please let me know if anyone
is planning on getting rid of or replacing their probe.

Thanks.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 8 Jun 2018 07:49:01 -0500
Subject: [Microscopy] viaWWW: Inverted Microscope Stand for Donation

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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD

Organization: Kimberly-Clark

Title-Subject: [Filtered] Inverted Microscope Stand for Donation

Message: Olympus IX70 stand with headpiece, no condenser, no stage
Available for donation
Excellent condition
Image upon request
Located in Wisconsin
Pickup or paid shipping only
Great base for building your own system!


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From: bethrichardson-at-uga.edu
Date: Fri, 8 Jun 2018 09:41:16 -0500
Subject: [Microscopy] Zeiss 1450EP SEM - two questions

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Hi all,
We have a Zeiss 1450EP SEM that is having an issue with the vacuum. It gives a vacuum error message - basically it doesn't want to recognize the vacuum hardware. Has anyone else experienced this problem with their scope? Any advice would be greatly appreciated. If we should just order a black wreath pleaselet us know that, too:)

We took the panels off the scopeand noticed a filter on the side of the scope. It contains anamber-colored substance that we'reassumingis similar todrierite. Does anyone know if we can recharge this filter by drying it in an oven? Can these still be ordered?

thanks for any advice,
Beth


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From: jquinn11733-at-gmail.com
Date: Fri, 8 Jun 2018 11:23:27 -0500
Subject: [Microscopy] re: Zeiss 1450EP SEM

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Beth and company -

After a fresh reboot of the computer, start your LEO application.
Screen print "Shift Prnt Scrn" the LEO-SRV.
Post that image to a public web-page. Then repost your query
with a link to the screen-dump.

I am looking to see if you get the "L-REM is not responding" or
"Vac firmware" message. However, anyone helping you (including
Zeiss service) would want to see all the messages.

As for the desiccant on the air line, just ask Zeiss service
for the part number. Mine is blue, but we use xdry-N2 for
the pneumatics.

regards,

- Jim

PS: OoO count is starting.............


--| Hi all,
--| We have a Zeiss 1450EP SEM that is having an issue with the vacuum. It
--| gives a vacuum error message - basically it doesn't want to recognize the
--| vacuum hardware. Has anyone else experienced this problem with their scope?
--| Any advice would be greatly appreciated. If we should just order a black
--| wreath please let us know that, too:)
--| We took the panels off the scope and noticed a filter on the side of the
--| scope. It contains an amber-colored substance that we're assuming is
--| similar to drierite. Does anyone know if we can recharge this filter by
--| drying it in an oven? Can these still be ordered?
--| thanks for any advice,
--| Beth

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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Jun 2018 17:18:24 -0500
Subject: [Microscopy] viaWWW:Bio TEM workshop July 25-27, 2018

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Title-Subject: [Filtered] Bio TEM workshop

Message: Biological TEM Workshop--
This intensive, three-day workshop will provide a practical and basic theoretical introduction to
the Transmission Electron Microscope and biological sample preparation techniques. Each day will
consist of lecture, discussion and *hands-on* training led by GEM staff. Who: Anyone requiring
training on TEM and biological sample preparation. The workshop will be limited to 6 participants
based on the availability of equipment. When: Wednesday through Friday, July 25-27, 2018, 8am-5pm
each day (lunch is provided)
Where: GEM labs, 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: July 20, 2018


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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Jun 2018 17:19:09 -0500
Subject: [Microscopy] viaWWW: EM UC7 Ultramicrotome 0.2mm arm retraction

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Title-Subject: [Filtered] EM UC7 Ultramicrotome 0.2mm arm retraction

Message: We are experiencing an issue with our Leica EM UC7 Ultramicrotome during the return stroke.
The instrument does not seem to be performing the 0.2mm retraction during the return stroke, causing
the previous cut sample to be picked back up by the knife edge. Does anyone know what adjustment
needs to be made and where (inside the instrument presumably). The instrument seemed to be operating
fine after the last PM by Leica, but now is doing the same thing as before the PM. If anyone has
drawings, a procedure, or a video that shows how to make the adjustment (or tighten a component), it
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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Jun 2018 17:19:48 -0500
Subject: [Microscopy] viaWWW: Searching for a carbon vacuum evaporator

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Title-Subject: [Filtered] Searching for a carbon vacuum evaporator

Message: I'm looking for a another carbon vacuum evaporator in good condition that would be
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From: steven.spurgeon-at-pnnl.gov
Date: Tue, 12 Jun 2018 11:01:48 -0500
Subject: [Microscopy] Registration Now Open for NexTEM Workshop 2018!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

We are pleased to inform you that registration for the NexTEM Microscopy Workshop is now open!

The inaugural Next-Generation Transmission Electron Microscopy (NexTEM) Workshop will be held October 8-10 in Discovery Hall at Pacific Northwest National Laboratory in Richland, WA. This event will include three full days of invited and contributed sessions on topics including:
· High-resolution imaging and spectroscopy
· In situ/operando microscopy in extreme environments
· Computational methods for data analysis.


NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions. The workshop will consist primarily of invited talks encompassing state-of-the-art developments and techniques, with a limited number of contributed talks and poster slots also available. These talks will take place during the first half of each day and will be open to all attendees.

An integral part of the workshop will be afternoon breakout and writing sessions, whose attendance will be limited to microscopy domain experts to facilitate active discussion. These sessions will cover the current state-of-the-art emerging advances, and the future of each microscopy sub-field. Importantly, we will encourage the exchange of ideas between breakout groups to identify potential areas of synergy and overlap. Breakouts will be led by two scientists with expertise in each domain who will be tasked with guiding the discussion, as well as outlining and writing. Once we have identified all the participants, we will contact potential breakout leads directly with more information prior to the workshop. We will prepare a perspective review article summarizing major conclusions from the NexTEM Workshop for the Journal of Materials Research in early 2019. All breakout participants will be listed as co-authors on this article.

During registration we are instructing all contributors to submit a two-page M&M style abstract for their talks/posters using the provided template (https://custom.cvent.com/5B9EB96FC2FC4AC69710004DEF407285/files/faf61a705bf64aea8af02becfad67c51.doc). If you have any questions, please don’t hesitate to contact us. We believe this will be an exciting event that will help define the future of electron microscopy and we greatly appreciate your participation.

Visit the NexTEM website (https://pnnl.cvent.com/nextem) for more information, to submit an abstract, and to register!

Workshop Organizers:

Steven Spurgeon
Staff Scientist
Pacific Northwest National Laboratory

Mitra Taheri
Hoeganaes Associate Professor
Drexel University

Workshop Coordinator:

Alyssa Cummings
Meeting and Event Consultant
Pacific Northwest National Laboratory
509-372-4883
nextem-at-pnnl.gov



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11, 38 -- Subject: Registration Now Open for NexTEM Workshop 2018!
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From: microscopy.listserver-at-gmail.com
Date: Tue, 12 Jun 2018 19:02:21 -0500
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
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Email: desertrat99-at-verizon.net
Name: Eric Rosen

Organization: UCLA

Title-Subject: [Filtered] Clinical EM Techs.
Message: Hiya all, just had a few question for the EM Techs who perform clinical work in hospitals.
I have asked this before many years ago and curious to know if things have changed.
How many specimens do you process and scope in your lab yearly?
How many techs are in the lab?
how many scopes do you have in the lab?
On a daily basis how many cases do your scope?
I will get things started.
Over here here we:

Process 1800 cases

2 Techs

Had 2 scopes but one is probably not repairable now

I scope myself about 7-10 a day

lately I scope all the cases in the lab and have done up to 10-15 a day.
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From: microscopy.listserver-at-gmail.com
Date: Wed, 13 Jun 2018 06:17:35 -0500
Subject: [Microscopy] viaWWW: M&M 2018 student bursaries

Contents Retrieved from Microscopy Listserver Archives
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Email: aml181954-at-gmail.com Name: Amanda Lawrence

Organization: -

Title-Subject: [Filtered] M&M 2018 student bursaries

Message: Reminder student opportunity at M&M 2018

The deadline for early registration is almost here and as you are making plans to attend the
Baltimore, MD meetings (Aug. 5-9) please don't forget about MSA's student bursary program. Its
purpose is to encourage students to attend the meetings by helping to defray some of the costs and
while giving them an opportunity to meet and interact with the established microscopy community.
The student bursaries will be paid $10 an hour to work for ~15-20 hours during the meeting and/or
pre-meeting events (paid by check at the end of the meetings). The jobs involve such things as
providing support in the different symposia, staffing the volunteer office, newsletter distribution,
and helping with vendor tutorial sign-up or in the outreach booth.
Once the program has been finalized, each registered bursary will be contacted and given the
chance to choose the times and activities they would like to help with. There is an additional
bonus of $10 cash for each morning and/or afternoon session worked to assist with meals and with a
meeting t-shirt to wear while serving as a bursary.
If anyone would like to participate in the bursary program, please contact the student council
(studentcouncil-at-microscopy.org) or Amanda Lawrence (aml181954-at-gmail.com) . Remaining bursary space
is limited, so sign-up soon. Participants are responsible for their own registration fee and travel
expenses.
Don't forget about the Pre-Meeting Congress for students on Sat. Aug. 4. Check with the student
council (studentcouncil-at-microscopy.org ) or the MSA website for details.

See everyone in Baltimore,


Amanda Lawrence
Student Bursary/Volunteer Coordinator
Microscopy Society of America
aml181954-at-gmail.com

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From: microscopy.listserver-at-gmail.com
Date: Wed, 13 Jun 2018 06:41:42 -0500
Subject: [Microscopy] viaWWW: EM UC7 Ultramicrotome 0.2mm arm retraction

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-------- Forwarded Message --------

X-from: Townley, Debra {debrat-at-bcm.edu}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Hi Clayton,

This won't be of any help to you, just a commiseration. My Leica UC7 is stuck. It won't reset, arm
won't retract and knife stage won't retract by using the coarse advance knob. I have 2 red lamps
that are winking at me, that's about it. The problem is intermittent and so far, unfixable.

:(
Debra Townley
Baylor College of Medicine

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X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, June
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To: Townley, Debra




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Email: cloehn-at-lsu.edu Name: Clayton Loehn

Organization: LSU

Title-Subject: [Filtered] EM UC7 Ultramicrotome 0.2mm arm retraction

Message: We are experiencing an issue with our Leica EM UC7 Ultramicrotome during the return stroke.
The instrument does not seem to be performing the 0.2mm retraction during the return stroke, causing
the previous cut sample to be picked back up by the knife edge. Does anyone know what adjustment
needs to be made and where (inside the instrument presumably). The instrument seemed to be operating
fine after the last PM by Leica, but now is doing the same thing as before the PM. If anyone has
drawings, a procedure, or a video that shows how to make the adjustment (or tighten a component), it
would be very appreciated
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From: microscopy.listserver-at-gmail.com
Date: Wed, 13 Jun 2018 18:10:36 -0500
Subject: [Microscopy] viaWWW:Searching for a Philips (FEI) EM208S Parts

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Email: desertrat99-at-verizon.net Name: Eric Rosen
Organization: UCLA
Title-Subject: [Filtered] Searching for a Philips (FEI) EM208S Parts

Message: Hi,
I am searching for some parts for a Philips (FEI)EM208S.
Specifically, looking for a computer for the microscope. It appears ours wants to freeze up after
loading the microscope interface.

I am not able to bypass this with a 3.5 inch boot disc to get into a DOS prompt to run some
diagnostics.

Anyone out there who has some advice or parts would be greatly appreciated.

Eric A. Rosen Electron Microscopist Dept. of Pathology and Laboratory Medicine
UCLA Medical Center
650 Charles E. Young Dr. South
Los Angeles, CA 90095


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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Jun 2018 18:20:11 -0500
Subject: [Microscopy] viaWWW:Camscan SEM vacuum problems

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Email: holpc-at-firstenergycorp.com Name: Chris Holp

Organization: FirstEnergy - BETA Lab

Title-Subject: [Filtered] Camscan SEM vacuum problems

Message: I am looking of assistance for a problem with our Camscan MV2300 SEM. About 17 years ago,
the electronics were replaced with Tescan Vega 1 controls. However, the column, chamber, and
diffusion pump vacuum system are original.

It has been displaying idiosyncrasies for some time now. Sometimes not venting completely, sometimes
pumping down very slowly. I recently replaced both upper and lower solenoid valves with OEM
replacements and refilled the diffusion pump with Santovac 5 oil. These steps did help quite a bit
but now there is something else.

My current problem is this: after venting, when I push the PUMP button to initiate roughing the
chamber out, the solenoid valves sequence, the vacuum draws on the door, but then the butterfly
valve opens right away also. I can't help but cringe when I hear the gulp of air surging down the
throat into the DP. Left alone, the system does reach good ultimate vacuum, but clearly this
situation is very bad and unacceptable. I'm not sure where to look or what to do to get the valve
sequencing correct.

Does anyone have familiarity with this scope who can provide some thoughts or advice? Any questions
will be readily answered!

Thank you.

Chris Holp
FirstEnergy - BETA Labs


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From: microscopy.listserver-at-gmail.com
Date: Fri, 15 Jun 2018 18:21:03 -0500
Subject: [Microscopy] viaWWW:need of a new printer

Contents Retrieved from Microscopy Listserver Archives
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From: hsia627-at-hotmail.com
Date: Fri, 15 Jun 2018 18:21:03 -0500
Subject: [Microscopy] Bio EM Sample Processing MiniCourse at University of Maryland

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,
We are pleased to announce that the Electron Microscopy Core Imaging Facility (EMCIF) at the
University of Maryland Baltimore will be offering a Biological EM Sample Processing mini-course on
July 12th and 13th, 2018 http://www.dental.umaryland.edu/UMBEMProcessingCourse/ .
The course is designed to teach any individual who wishes to learn biological sample processing for
electron microscopy. No experience is required. The course will be limited to six participants.

--Course format: lectures, demonstrations and hands on practice.
--Topics: Fixation, resin embedding, conventional and modern rapid processing methods, microwave
assisted processing, automated sample processing, SEM biological sample processing and Negative
staining of particulate specimen.

--Instrument Demonstration: critical point dryer, ASP1000 (Microscopy Innovations), Biowave
(TedPella), vibrotome

--Other related courses: EMCIF also offers room temperature ultramicrotomy and immunogold labeling
minicoures in September and October this year. The minicourses follow a similar format covering
principles, practices, and troubleshooting with an emphasis on hands-on practice and instrument
demonstration.
--More information: Email coreimaging-at-umaryland.edu or visit our website
http://www.dental.umaryland.edu/core-imaging/workshops-and-courses/ Thanks.

Sincerely,

Ru-ching Hsia, Ph.D.
Associate Professor and Director
Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201
Ru-ching Hsia

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From: Christoph.Bauer-at-unige.ch
Date: Fri, 22 Jun 2018 08:24:41 -0500
Subject: [Microscopy] TEM: Job opening for a cry-TEM specialist at the University of Geneva

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Section of Biology in the Faculty of Sciences at the University of Geneva has a long history in electron microscopy. Indeed, the first Swiss-made electron microscope was built and installed here in the 1940's. Presently, the Section is renewing its Electron Microscopy infrastructure to better support the work of current and future groups. The main function of the facility will be to help users in all aspects of the (Cryo-) EM workflow.
To support this facility, we are looking for a

ELECTRON MICROSCOPY PLATFORM SPECIALIST

The specialist will participate in all technical aspects of the facility, including use, maintenance and upkeep of the electron microscopes and associated equipment, support for image processing and some managerial tasks.
He/She will be expected to collaborate with users in specimen preparation (cryo-EM or negative stain), operation of the microscopes and associated equipment, instrument training, experimental design, data processing/analysis/storage, interpretation of results, and updating users on the latest methodologies through familiarity with relevant literature.
REQUIREMENTS:
The successful candidate will have a Ph.D. degree in biology, biochemistry or a related field and must have a minimum of 5 years of hands-on experience in cryo-electron microscopy.
He/She must have strong communication skills, be detail-oriented, focused, highly motivated and have the ability to work collaboratively in a team as well as independently on a wide variety of research projects. A strong background in computation would be a plus.
English is the working language.
ASSIGNMENT: Full-time appointment
STARTING DATE: December 1st, 2018, or as agreed
Applications should be submitted on-line through our website: https://jobs.unige.ch
Complementary information may be by e-mail address (see below)


BIOIMAGING CENTER
University of Geneva - Science II
Room 245
30, Quai Ernest Ansermet
CH - 1211 Genve 4

Dr. Christoph Bauer
Managing Director
Tel.: + 41 22 3796632
Fax: + 41 22 3796868
email: Christoph.Bauer-at-unige.ch
website: http://bioimaging.unige.ch/



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From: sergei2-at-ornl.gov
Date: Fri, 22 Jun 2018 10:55:21 -0500
Subject: [Microscopy] PyCroscopy: CNMS User Meeting Workshop

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

CNMS User Meeting Workshop, August 13-15

Dear Colleagues - we are hosting two workshops that will run
concurrently with the CNMS User Meeting, August 13-15, at ORNL.
Registration is free, but advanced registration is required for attendance.

On August 13, we will go through imaging and spectral data analysis
through our open-source pyCroscopy platform. This will include tutorials
on storing data through hdf5 containers, and performing multidimensional
analysis and visualization.

On August 15, we will introduce machine learning methods and show use
cases for commonly acquired materials science data. This workshop will
also include a short introduction to deep learning.

Both workshops will be hands-on and emphasize use, rather than lectures.
We are exploring options for streaming, for those who cannot attend on
site. Space is limited! See here for details

https://cnmsusermeeting.ornl.gov/announcement/

and register here:

https://www.ornl.gov/facility/cnms/output/register-now-august-13-15-2018-cnms-user-meeting

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Thu, 28 Jun 2018 18:13:06 -0500
Subject: [Microscopy] viaWWW:Tecnai G2 20 Twin Coefficients

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Email: katayoun.mansouri-at-duke.edu Name: Katayoun Mansouri

Organization: Duke University

Title-Subject: [Filtered] Tecnai G2 20 Twin Coefficients

Message: Hello,
Does anybody know what are the spherical (Cs) and chromatic (Cc) aberration
coefficients of objective lens for an FEI Tecnai G2 20 Twin, operating at maximum 200kV and LaB6
filament?
Thank you,

Katayoun


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From: microscopy.listserver-at-gmail.com
Date: Thu, 28 Jun 2018 18:13:57 -0500
Subject: [Microscopy] viaWWW: Ted Pella - Life Sciences Product Specialist Opening

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Email: bonnie_salyer-at-tedpella.com Name: Bonnie Salyer

Organization: Ted Pella, Inc.

Title-Subject: [Filtered] Life Sciences Product Specialist

Message: Ted Pella, Inc. is seeking candidates for Life Sciences Product Specialist to develop and
grow TEM life science-related products, Histology products, Light Microsopy products and supplies,
gold and Nanoparticle lines, and Neuroscientific product lines.
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From: m.aindow-at-uconn.edu
Date: Fri, 29 Jun 2018 03:26:52 -0500
Subject: [Microscopy] Staff Position in Electron Microscopy at UConn

Contents Retrieved from Microscopy Listserver Archives
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Posting Title:  Microscopy Specialist (Academic Assistant 3)

The Institute of Materials Science (IMS) at the University of Connecticut (UConn) is an interdisciplinary institute with a threefold mission fostering education, research, and outreach in all areas of the materials sciences.
IMS is seeking qualified applicants to fill the position of Microscopy Specialist (Academic Assistant 3) in the new UConn-Thermo Fisher Scientific Center for Advanced Microscopy and Materials Analysis (CAMMA). The Center was formed as a partnership between UConn and Thermo Fisher Scientific to serve the analytical needs of industrial and academic researchers. The Center has acquired seven new electron beam instruments including state-of-the-art TEM, SEM and FIB systems. These are housed in the UConn Technology Park as part of the purpose-built Advanced Characterization Laboratory, which opened earlier this year.

DUTIES AND RESPONSIBILITES
Working under the direction of the Center Director, the Microscopy Specialist will have responsibility for the CAMMA activities in the areas of aberration-corrected scanning transmission electron microscopy (STEM), with particular emphasis on in situ experiments and electron tomography. Duties will include: supervising the staff and students who work in the Center; overseeing the operation, maintenance and user training on the instruments; managing systems for user booking and logging of instrument usage.

MINIMUM QUALIFICATIONS
An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A minimum of five years’ experience involving the use of aberration-corrected scanning transmission electron microscopes. Good written and verbal communication skills.  Strong interpersonal skills including the ability to interact effectively with faculty, staff, students and customers from industry.

PREFERRED QUALIFICATIONS
Experience with FEI instruments, including: aberration-corrected TEMs, small dual-beam FIB systems and SEMs.  Experience with in situ stages for STEM (heating, gas-cell, liquid cell and biasing). Experience with electron tomography. Strong track record of working with industry.

APPOINTMENT TERMS
This is a full time, 11-month position. Salary is commensurate with background and experience and includes a full benefits package.

TO APPLY
Please send a letter of application, resume, and the names of three professional references, including contact information via UConn Jobs, http://www.jobs.uconn.edu, Staff Positions. Please reference Search # 2018605 when applying.  Screening will begin immediately and this search will remain open until a suitable candidate is found.  Employment of the successful candidate is contingent upon the successful completion of a pre-employment background check. (Search # 2018605).
This job posting is scheduled to be removed at 11:59 p.m. Eastern time on July 21, 2018.
All employees are subject to adherence to the State Code of Ethics which may be found at http://www.ct.gov/ethics/site/default.asp.


The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty and staff. The diversity of students, faculty and staff continues to increase, as does the number of honor students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and instates serve the University’s teaching, research, diversity, and outreach missions, leading to UConn’s ranking as one of the nation’s top research universities. UConn’s faculty and staff are the critical link to fostering and expanding our vibrant, multicultural and diverse University community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities and members of traditionally underrepresented populations.




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==============================End of - Headers==============================




From: P.J.Fletcher-at-bath.ac.uk
Date: Fri, 29 Jun 2018 06:22:49 -0500
Subject: [Microscopy] Job adverts for the microscopy list server

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Microscopy.com,
Please can the following two job adverts be added to the list server.
Thanks,
Dr. Philip Fletcher,
Microscopy and Analysis Suite, University of Bath.


JOB 1:
SUBJECT: Instrument Specialist position in Bio Electron Microscopy at the University of Bath
Faculty of Science
Salary: Starting from 32,548, rising to 38,833
Placed On: Friday 22 June 2018
Closing Date: Tuesday 24 July 2018
Interview Date: To be confirmed
Reference: CA5943
Applications are invited for the post of Instrument Specialist in the Material and Chemical Characterisation Facility (MC2). MC2 provides comprehensive microscopy and analytical facilities to researchers across the University. Applicants should have an in-depth understanding and extensive practical experience of electron microscopy (SEM, Cryo-FESEM, TEM), x-ray analysis (EDX) and biological sample preparation methods. The ability to work as part of a team is essential. Duties will involve providing a service to researchers in all aspects of electron microscopy including maintaining and monitoring equipment, and interpreting instrument output (data/images). A state-of-the-art Cryo-FESEM with EDX and STEM has recently been purchased and the instrument specialist will be expected to learn all aspects of the operation of this new instrument. This post is an open-ended contract. Full details of the role itself and the person specifications can be accessed through the link below.
http://www.bath.ac.uk/jobs/Vacancy.aspx?ref=CA5943

JOB 2:
SUBJECT: Instrument Specialist - BioImaging at the University of Bath
Faculty of Science
Salary: Starting from 32,548, rising to 38,833
Placed On: Friday 22 June 2018
Closing Date: Monday 23 July 2018
Interview Date: To be confirmed
Reference: CA5945
Applications are invited for the post of Instrument Specialist in the Material and Chemical Characterisation Facility (MC2). MC2 provides comprehensive bio-imaging facilities to researchers across the University.
Applicants should have an in-depth understanding and extensive practical experience of confocal laser scanning microscopy (CLSM) and fluorescence activated cell sorting (FACS). Experience of working with a CLSM plus a multiphoton laser and/or a high content microscope would be an advantage. The ability to work as part of a team is essential.
Duties will involve providing a service to researchers in CLSM, FACS, and use of hypoxic facilities including maintaining and monitoring equipment, and interpreting instrument output (data/images). A state-of-the-art CLSM with multiphoton laser has recently been purchased and the instrument specialist will be expected to learn all aspects of the operation of this new instrument.
This post is an open-ended contract. Full details of the role itself and the person specifications can be accessed through the link below.
http://www.bath.ac.uk/jobs/Vacancy.aspx?ref=CA5945



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From: microscopy.listserver-at-gmail.com
Date: Tue, 3 Jul 2018 06:43:17 -0500
Subject: [Microscopy] viaWWW: Job Posting: Cellular Biologist / Cell Structure

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Email: kacharb-at-nidcd.nih.gov Name: Bechara Kachar

Organization: NIDCD, NIH

Title-Subject: [Filtered] Job Posting: Cellular Biologist / Cell Structure
Message: Dear colleagues,
We are currently seeking a Cellular Biologist / Cell Structure Expert for a position within the
Laboratory of Cell Structure and Dynamics at the NIDCD, NIH.

Research area: Structure and regulation of stereocilia and other model actin-based cellular
protrusions

Requirements: Experience in cellular biology, cutting edge fluorescence microscopy, and/or
cryo-electron tomography.

For details about the position and to apply visit:
https://kelly.secure.force.com/CandidateExperience/CandExpJobDetails?id=a7V80000000UM3VEAW&searchFlag=true&tid

For details about the Laboratory and research program please visit:
http://www.nidcd.nih.gov/research/scientists/pages/kacharb.aspx

For further inquiries please contact me directly.
Best regards,
Bechara
----------------------------------------------------
Bechara Kachar, M.D.
Chief, Laboratory of Cell Structure and Dynamics
NIDCD, National Institutes of Health
Porter Neuroscience Research Center
35A Convent Drive, Room 3D-824
Bethesda, MD 20892
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From: microscopy.listserver-at-gmail.com
Date: Tue, 3 Jul 2018 06:44:09 -0500
Subject: [Microscopy] viaWWW:Looking for Mag Poweramp for JSM-6400

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Email: steven.cogswell-at-gmail.com Name: Steven Cogswell

Organization: UNB

Title-Subject: [Filtered] Looking for Mag Poweramp for JSM-6400

Message: Hi folks;

My venerable jeol jsm-6400 (tungsten) sem has developed some nasty image distortions (crt and
recorded with digiscan), and we think it's probably our good friend the mag pwramp box. I'm trying
to get a replacement for it, and jeol does not have one.
This is an open-frame assembly board but it's in the gold-box-waterblock in the bottom of the
instrument. My schematic book lists it as 806512822. It's probably in a lot of different jsm
machines. I can provide more details if you want it.
Do any of you nice folks have one you're willing to part with?

Best regards,
Steven Cogswell
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From: stefan.diller-at-t-online.de
Date: Wed, 4 Jul 2018 13:55:26 -0500
Subject: [Microscopy] Jeol 820 HT Generator Board needed

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

after setting up a Jeol 820 SEM at a German highschool I do have the following problem:

- no higher acceleration voltage possible than 2.9 kV

Switching to 3kV on the digi switches causes the emission to become unstable and fluctuating in a very large range.

Below 3kV all is fine.

HV tank itself seems to be OK. I had it open and nothing appeared to be out of position.

HV cable is also OK. The problem is nearly the same without cable (fluctuations are existing, but since no emission, only ca. 10%
of former value...).

I suspect that either the board logic becomes flawed after using the 3kV setting. It seems like the HV changing values very fast
but there is no direct readout to check for. Or the bias. I am not sure.

Maybe somebody in the group has a HV tank available or the HT generator board, Jeol number AP00188(01) ?

Here are some images of the board needed:

https://drive.google.com/drive/folders/10bna0tljJedsIu90ORQHCEkyDG63MiKd?usp=sharing


Best wishes,

Stefan





--


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Websites:
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www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
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From: microscopy.listserver-at-gmail.com
Date: Sun, 8 Jul 2018 19:44:16 -0500
Subject: [Microscopy] viaWWW: Microscopy and Microanalysis EMLG FIG

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Email: unocicrr-at-ornl.gov Name: Raymond Unocic

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] Microscopy and Microanalysis EMLG FIG

Message: Microscopy and Microanalysis Electron Microscopy in Liquids and Gases Focused Interest Group

Dear M&M Attendees:

On behalf of the EMLG FIG we would like to bring to your attention to two events for researchers
that are interested in the field of in situ/operando microscopy in liquids and gases:

1) Pre-meeting Congress X62 meeting (Practical Challenges and Opportunities for in situ/operando
microscopy in liquids and gases) that will be held on Sunday August 5, 2018 at the Annual M&M2018
meeting in Baltimore Maryland. Please come hear about the latest advances in this field from an
exciting lineup of invited speakers including:

Bryan Reed (IDES, inc.), See Wee Chee (NUS), Katherine Jungjohann (Sandia National Laboratory), Alex
Belianinov (Oak Ridge National Laboratory), Eric Stach (University of Pennsylvannia), Lena
Kourkoutis (Corness), Alex Liddle (NIST), Xiaoqing Pan (UC Irvine), Renu Sharma (NIST), Stephen Mick
(Gatan)
Registration cost: $159 (Members), $209 (Non Members), $75 (Students)

2) Our EMLG FIG will also hold our business meeting on Tuesday August 7, 2018 at 12:15 p.m. (Room
TBD) Our leader elect (Dr. Houlin Xin) will be assuming leadership responsibilities for 2018-2020
and will be holding an election for our leader elect position (who will take over the FIG in 2020)
and sec/treasurer. In order to attend the meeting and be eligible to vote, it is important to have
your $10 membership dues paid in full. The membership fees can be paid through your MSA account or
when you register for the conference.
See you in Baltimore :)
Ray Unocic

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From: microscopy.listserver-at-gmail.com
Date: Sun, 8 Jul 2018 19:45:18 -0500
Subject: [Microscopy] viaWWW:Associate Staff Scientist to manage FIB laboratory at U

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Email: tzega-at-lpl.arizona.edu Name: Tom Zega

Organization: University of Arizona

Title-Subject: [Filtered] Associate Staff Scientist to manage FIB laboratory at U Arizona

Message: Colleagues,

I'd like to draw your attention to an open position for Associate Staff Scientist at the University
of Arizona to manage a FIB and SEM laboratory.

} From the job description:

The Kuiper Materials Imaging and Characterization Facility (KMICF) at the University of Arizona
invites applications for the position of Associate Staff Scientist. KMICF is part of a network of
core analytical facilities and is dedicated to imaging, spectroscopy, and analysis of heterogeneous
materials. Laboratories include electron microprobe, scanning electron microscopy, transmission
electron microscopy, and focused ion beam electron microscopy. The staff scientist will work with
the instrument scientist and will be responsible for managing daily operations of the
focused-ion-beam and scanning electron microscope laboratories.

Please visit https://uacareers.com/postings/30631 for more details and to apply.

Thank you.

Tom Zega

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From: microscopy.listserver-at-gmail.com
Date: Sun, 8 Jul 2018 21:40:41 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:need of a new printer

Contents Retrieved from Microscopy Listserver Archives
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X-from: Bill & Sue Tivol {wtivol-at-sbcglobal.net}




} On Jun 15, 2018, at 4:23 PM, microscopy.listserver-at-gmail.com
} {mailto:microscopy.listserver-at-gmail.com} wrote:
}
} Message: I am in need of a new printer, for B&W prints (biological TEM) and color prints (scanned
} H&E slides). Your recommendations would be appreciated.
}
Dear Cynthia,
Some time ago a contributor to the list recommended the Epson Stylus C88+, which I subsequently
bought and am very happy with. It may no longer be available, but perhaps its successor is equally
good.
Yours,
Bill




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From: microscopy.listserver-at-gmail.com
Date: Tue, 10 Jul 2018 06:43:24 -0500
Subject: [Microscopy] viaWWW: Postdoctoral Research Associate, Environmental

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Email: pierceem-at-ornl.gov Name: Eric M. Pierce

Organization: Environmental Sciences Division/Oak Ridge National Laboratory

Title-Subject: [Filtered] Job Posting for Postdoctoral Research Associate, Environmental
Biogeochemistry / NB50639999

Message: Company: The Environmental Sciences Division, http://www.esd.ornl.gov, at Oak Ridge
National Laboratory (ORNL), http://www.ornl.gov, is seeking to fill a postdoctoral position in the
area of environmental biogeochemistry. The project is aimed at understanding the interfacial
processes between mineral surfaces, trace metals, natural organics, and microorganisms. You will be
involved in a multidisciplinary research team investigating coupled geochemical, biological, and
environmental processes that influence the chemical speciation, biological uptake and transformation
of contaminants (such as mercury) in water and sediments. Particular interest is the application of
high-resolution imaging tools to improve our understanding of processes that influence trace metal
speciation at the interfaces between minerals-microorganisms and minerals-organic matter. You should
have experience necessary to initiate laboratory studies, which include, but are not limited to,
developing or refining research ideas, setting up laboratory experiments, collecting experimental
data, data analysis, writing journal publications, and presenting at group and scientific meetings.
Experience with the use of high-resolution microscopy tools in the context of biogeochemistry or
material science is a plus but not required. The selected candidate will work closely with Earth
Sciences Group leader, other Earth Sciences group members, and also collaborate closely with other
scientist at ORNL and collaborating institutions.

Major Duties/Responsibilities:
You will have the responsibility to design and conduct laboratory experiments, maintain detailed
scientific records, analyze experimental data, present results within the research team and at
national and international conferences, and prepare manuscripts for publication in peer-reviewed
journals. The nature of our scientific work is highly multidisciplinary, and projects will be
carried out in close collaboration with scientists from a variety of scientific backgrounds,
including geochemistry, microbiology, biochemistry, physical chemistry, or a related field to
investigate the biochemistry of contaminant transformations.

Qualifications Required:
• PhD in environmental chemistry, geochemistry, geomicrobiology, physical chemistry, material
science, or related discipline. • Experience with advanced analytical microscopy techniques, such as
electron microscopy and electron energy loss spectroscopy, is highly desired • You cannot have
received the most recent degree more than five years prior to the date of application and must
complete all degree requirements before starting your appointment. • You should demonstrate the
ability to work independently and collaboratively as part of a large, multidisciplinary team and
should be able to design and execute experiments, perform data analyses and interpretation of
experimental results. • Strong record of productive and creative research demonstrated by
publication in peer-reviewed journals and presentations at scientific conferences, and experience
with environmental biogeochemistry or material science are expected. • Excellent interpersonal,
oral, and written communication skills are required.

Appointments will initially be for 24 months with a possibility of an extension of up to 12 months.
Initial appointments and extensions are subject to performance and availability of funding.

Relevant Websites:
ORNL Earth Sciences Group: https://www.ornl.gov/division/esd/earth-sci ORNL Environmental Sciences
Division website: https://www.ornl.gov/division/esd Primary Research Program website:
https://www.esd.ornl.gov/programs/rsfa/

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From: zaluzec-at-microscopy.com
Date: Wed, 11 Jul 2018 08:57:08 -0500
Subject: [Microscopy] In Memoriam: Theodore Pella

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-------- Forwarded Message --------

X-from: Tom Pella {tom_pella-at-tedpella.com}


With a heavy heart, I write this announcement that on Friday, June 29th, at
9:01 PM, the co-founder and namesake of Ted Pella, Inc., Ted Pella, died and
went to glory. He died on his 91st birthday from complications due to
Alzheimer's Disease, at Oakmont of Redding Retirement & Assisted Living
Facility. He died with his wife Christel at his side.


He was preceded in death by his daughter Stephanie Pella Mills and his
grandchild Christen Joy, and is survived by his wife Christel; his sister
Alice Pella Prong; his son Thomas and daughter-in-law Diane; and his three
grandchildren, Claire, Cornelia and Cameron.


Ted was born on June 29th, 1927 to the late Rudolf and Mary Pella in East
Rochester, NY. Ted's character and spirit were molded by the kindness of his
mother, a devout Catholic. He grew up in the Great Depression and while his
family was poor, he never knew that, enjoying the simplicity of being around
family and friends. While growing up he worked in the family grocery store.
It was there that Ted's passion for serving his customers was first planted
and became a guiding principle wherever he worked.


Ted was a starter on the High School basketball team, was voted most likely
to succeed, and graduated High School as the valedictorian of his class. But
he skipped the ceremony and instead signed up for the Navy early in the
summer of 1945. The war was ending, however, and the closest he got to the
front lines was working in a discharge center in Memphis, TN.

His brief time in the service entitled him to attend college, and he
returned home to attend the University of Rochester, getting a Bachelor's
degree in Physics in 1950. Upon graduation he moved to New York City and
found temporary quarters at the YMCA. Shortly afterwards he saw a job
posting there on an index card for a Sales Engineer for the Ivan Sorvall
Company which was designing and selling laboratory instrumentation. This was
a perfect fit for Ted, who was looking for something that combined business
and science.

He went to work in field sales and service for Sorvall. Ted delivered the
first commercial ultramicrotome ever sold, under his arm to the Rockefeller
Institute in New York, taking a train to get there. He greatly enjoyed
visiting with customers, helping repair instruments in their labs and
finding ways to help. Not long after joining Sorvall, Mr. Sorvall passed and
the company was bought by LKB, another scientific instrument company, based
out of Sweden. Ted continued his sales work for LKB and began to rise in the
ranks until he oversaw sales for the USA.

It was during this period in 1956 that LKB sent him to Sweden for further
instrument training. The person conducting the training was a TEM Technician
at Karolinska Institute named Christel, and thus began a relationship which
led to Christel emigrating to the USA, and their getting married in 1957.
They were married for 60 years, celebrating that anniversary last year.


Ted was eventually promoted to VP of Sales at LKB, and the family moved to
Sweden in 1966. But Ted was not a good fit for the Swedish culture and the
home office. During a vacation Ted bounced the idea off Chris: Why don't we
start our own business back in the states? Chris had a spirit of adventure
and they agreed to start it together. The family moved back to the states in
late 1967, this time to Southern California where on Jan. 1, 1968 Ted Pella,
Inc. was started.


The business started in their garage and den. They soon hired their first
employee at home to help type; and a few years later moved into their first
office space. The company slowly grew and added employees, and they worked
many hours to make ends meet and meet the needs of their customers.


The time finally came when they wanted to own their own building; but this
was the time of the great real estate boom of the 1980's and no land was
easily available nearby in Orange County for building. The City of Redding,
California eventually "found" them at a trade show in 1986 and introduced
them to what Northern California had to offer. Ted & Chris moved the company
to Redding in the Spring of 1987. At that time the number of employees had
grown to 19.


Ted & Chris continued to own and manage the company for 44 years, until 2010
when the number of employees was over 60. During this time the company grew
to one of the premier suppliers of microscopy equipment and supplies
worldwide. But Ted and Chris always kept their focus on serving the needs of
scientists. In 2010 they passed ownership and management to their son Tom,
but continued to be involved into their mid 80's until they finally retired
in 2014, having served the microscopy community for over 60 years. Even at
the 2017 Neurosciences show a number of customers asked how Ted was doing,
having enjoyed meeting him at the booth in a trade show.


The company will observe a special half-day holiday in Ted's honor and close
tomorrow, Wednesday, July 11th, in the afternoon to hold a Memorial Service
and reception at the Dignity Memorial McDonald's Chapel in Redding for
employees and friends. If anyone wishes to express their condolences to
Christel and post a note about Ted for that event, please send them to
tom_pella-at-tedpella.com and it will be included in an album being presented
later to Christel.


Sincerely,


Tom Pella

President

Ted Pella, Inc.

Redding, CA 96003

P: 530-243-2200

F: 530-243-3761

|--http://www.tedpella.com--| www.tedpella.com





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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 Jul 2018 07:14:21 -0500
Subject: [Microscopy] viaWWW:What LIMS are you using for microscopy ?

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Phillips, Thomas E. {PhillipsT-at-missouri.edu}
CC: alexandre.bastien-at-fsaa.ulaval.ca {alexandre.bastien-at-fsaa.ulaval.ca}


------- Forwarded Message --------


X-from: alexandre.bastien-at-fsaa.ulaval.ca

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Email: alexandre.bastien-at-fsaa.ulaval.ca Name: Alexandre Bastien

Organization: Universit Laval

Title-Subject: [Filtered] What LIMS are you using for microscopy ?

Message: Hi all,

I'm looking for a laboratory information management system (LIMS) for microscopy. I don't need all
the online analysis provided by OMERO, and I'm looking for something simpler. Many of our students
just save their data on our server with stupid names like test123. I'm looking for a way to stick a
protocol next to every image taken in a clean searchable way. For example, if the image is
immunofluo of a bovine embryo, I would want the user to select from a list of protocols, then enter
specie, cell type, antibody, wavelength, etc. The actual microscope settings are not so important
because they are already stored in the metadata. The best solution would be something open source
that we could run locally on our servers.

Many thanks!

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From: colijn.1-at-osu.edu
Date: Thu, 12 Jul 2018 09:48:03 -0500
Subject: [Microscopy] CryoTEM positions available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The CEMAS facility at Ohio State University has 2 positions available in CryoTEM. See the links below for details.

Cryo-EM Senior Research Associate
https://www.jobsatosu.com/postings/87229

Cryo-EM Research Associate
https://www.jobsatosu.com/postings/87225


Cheers,
Henk


Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu


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From: sergei2-at-ornl.gov
Date: Thu, 12 Jul 2018 13:43:49 -0500
Subject: [Microscopy] =?UTF-8?Q?Postdoctoral_Research_Associate_=e2=80=93_Physics_Analyti?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

We are seeking a Postdoctoral Research Associate to support our efforts
in Scanning Transmission Electron Microscopy and Scanning Probe
Microscopy Groups on the mathematical aspects of physics extraction from
spatially resolved imaging of dynamic systems. Our team aims to
establish a comprehensive means of feature extraction, conversion of
dynamic imaging data to materials specific descriptors, and recovery of
underpinning physical behaviors. As a postdoc, you will help us
transition from the atomically-resolved and mesoscopic scanning probe
and electron microscopy data to underlying physics laws, via either
mesoscopic dynamic models or atomistic dynamics. You will be
collaborating with an interdisciplinary research team to develop machine
learning methods for rapid, on-the-fly analytics of imaging and spectral
imaging data, and then to implement these methods and algorithms for
physical analysis.

This position resides in the Center for Nanophase Materials Sciences
Division (CNMS), Physical Sciences Directorate (PSD) at Oak Ridge
National Laboratory (ORNL).

In this position you will:
• Develop and adapt algorithms and software for materials specific
feature extraction from atomic and mesoscopic imaging
• Develop approaches for physics extraction from imaging data using
inference, information compression, manifold learning, representation
learning, and similar methods
• Collaborate with team members within and outside of electron, scanning
probe, and other imaging groups at ORNL
• Present and report research results and publish scientific results in
peer-reviewed journals

Basic Qualifications/ To be considered you must have:
• A PhD in Computer Science and Engineering, Applied Mathematics,
Computational Physics, or closely related field completed within the
last five years
• A track record of excellent analytical and problem solving skills
• Experience with Python and the associated scientific software library
stack
• A strong record of productive and creative research demonstrated by
publications in peer-reviewed journals and/or presentations at
scientific conferences

Preferred Qualifications/ This experience is to your advantage:
• Background in data analysis and simulations using Python or Matlab
• Excellent written and oral communication skills and the ability to
communicate in English to an international scientific audience
• Motivated self-starter with the ability to work independently and to
participate creatively in collaborative and frequently interacting teams
of researchers
• Ability to function well in a dynamic research environment, set
priorities to accomplish multiple tasks within deadlines, and adapt to
ever-changing needs

Contact Sergei Kalinin (sv9-at-ornl.gov) for additional information.

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, FPresight Institute, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


==============================Original Headers==============================
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From: xin-at-magnet.fsu.edu
Date: Thu, 12 Jul 2018 14:28:51 -0500
Subject: [Microscopy] nital solution storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I am seeking advice and suggestions on how to keep nital chemical solution
(33%nitric acid in methanol) in the lab. We use this solution quite often
to make metal TEM samples. However, the university safety officer does not
allow us to keep it in the lab for future use. They requested us to
dispose it after one time use. We never had issues with the old solutions.
We are trying to persuade the safety office to let us keep this solution
in the lab for at least several months.
I would really appreciate your input and suggestions and the protocol on
how your lab handle and store this solution.

Thanks
Yan Xin
NHMFL/FSU

==============================Original Headers==============================
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3, 121 -- From: Yan Xin {xin-at-magnet.fsu.edu}
3, 121 -- To: {microscopy-at-microscopy.com}
3, 121 -- Subject: nital solution storage
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From: nicholls-at-uic.edu
Date: Thu, 12 Jul 2018 16:17:52 -0500
Subject: [Microscopy] nital solution storage

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Yan Xin

I agree with your safety officer. 33% Nitric in Methanol is an unstable explosive mixture. Nitric acid can react with methanol to produce methyl nitrate which is a colorless volatile liquid that is explosive.

Typically any solution of Nital (nitric acid/ alcohol) over 5% nitric should not be stored.

I saw the results of this many years ago while a graduate student. Even stored in a fridge a bottle of 30% nitric in methanol exploded overnight.

Regards

Alan

Alan Nicholls, PhD
Associate Director, RRC
Director, Electron Microscopy Service
Research Resources Center
The University of Illinois at Chicago
845 West Taylor Street
110, SES, MC337
Chicago, IL 60607
(312) 996-1227
nicholls-at-uic.edu



-----Original Message-----
X-from: xin-at-magnet.fsu.edu [mailto:xin-at-magnet.fsu.edu]
Sent: Thursday, July 12, 2018 2:31 PM
To: Nicholls, Alan W {nicholls-at-uic.edu}

Dear Colleagues,

I am seeking advice and suggestions on how to keep nital chemical solution (33%nitric acid in methanol) in the lab. We use this solution quite often to make metal TEM samples. However, the university safety officer does not allow us to keep it in the lab for future use. They requested us to dispose it after one time use. We never had issues with the old solutions.
We are trying to persuade the safety office to let us keep this solution in the lab for at least several months.
I would really appreciate your input and suggestions and the protocol on how your lab handle and store this solution.

Thanks
Yan Xin
NHMFL/FSU

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3, 121 -- From: Yan Xin {xin-at-magnet.fsu.edu} 3, 121 -- To: {microscopy-at-microscopy.com} 3, 121 -- Subject: nital solution storage 3, 121 -- Date: Thu, 12 Jul 2018 15:30:35 -0400 3, 121 -- Message-ID: {cf6bc745.00001664.0000008d-at-xin-980.ad.magnet.fsu.edu}
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From: baskin-at-bio.umass.edu
Date: Thu, 12 Jul 2018 17:59:38 -0500
Subject: [Microscopy] RMC propane jet freezer

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues,
Does anyone have a user's manual for the RMC MF-7200
Cryo Propane Jet Ultrarapid Freezer? Or spare parts for that instrument
they would be willing to loan, give, or sell, including but not limited
to, the control cable, a sample holder, a sample port plug, perhaps
hoses/connectors for N2 gas and liquid nitrogen? I am posting this on
behalf of my colleague Larry Winship, who is not a member of the list.
Please reply to him ljwNS at hampshire.edu.

Thanks,
Tobias

==============================Original Headers==============================
2, 23 -- From baskin-at-bio.umass.edu Thu Jul 12 17:59:38 2018
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2, 23 -- Larry Winship {ljwNS-at-hampshire.edu}
2, 23 -- From: Tobias Baskin {baskin-at-bio.umass.edu}
2, 23 -- Subject: RMC propane jet freezer
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==============================End of - Headers==============================




From: colijn.1-at-osu.edu
Date: Sun, 15 Jul 2018 20:16:07 -0500
Subject: [Microscopy] Job opening at Ohio State University - Center for Electron Microscopy and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

We have an opening for an SEM instrument manager at the CEMAS facility.
The details are available in the link below.

https://www.jobsatosu.com/postings/88084

Cheers,
Henk


--------------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100, Columbus, OH 43212

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

"Time is that quality of nature which keeps things from happening all at
once." (Ray Cummings - 1922)
Lately it doesn't seem to be working.



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11, 123 -- Subject: Job opening at Ohio State University - Center for Electron Microscopy and
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From: microscopy.listserver-at-gmail.com
Date: Tue, 17 Jul 2018 20:17:48 -0500
Subject: [Microscopy] viaWWW: Job Opening : sales representative in Ontario

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: stefano-at-soquelec.com


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Email: stefano-at-soquelec.com Name: Stefano Rubino

Organization: Soquelec Limited

Title-Subject: [Filtered] job offer: sales representative in Ontario

Message: Start date: as soon as possible

Hourly/yearly wage: competitive, to be discussed

Job type: Permanent full-time

Relocation/travel requirements: position based in Toronto/Ottawa with up
to 80% travelling

Soquelec Ltd., founded in 1974, is the Canadian distributor of
consumables and equipment for high-end imaging, analysis, and sample
preparation research laboratories across Canada.

Job Summary

The ideal candidate would be responsible for the sales and, possibly,
service of scientific equipment from a variety of suppliers (TESCAN,
Gatan, Quorum, Bruker, etc.), as well as establish and maintain strong
business relationships with our current and potential customers in
Ontario and the Maritime provinces. You will be working with the rest of
the sales team and the administrative staff closely to achieve your
sales goals and contribute to company objectives.

Key Responsibilities

Initiate contacts with potential customers and qualify sales
opportunities;
Follow up and close sales opportunities in a timely manner;
Update internal CRM regularly of any relevant task, activity or note;
Train end users on applicable instruments;
Build and facilitate positive and productive customer relationships;
Participate in application trade shows and conferences;
Participate in applicable training opportunities.

Requirements

Bachelor’s Degree in Science or Engineering;
Excellent communication skills in English;
Prior experience in electron microscopy and/or x-ray imaging;
Excellent sales ability and customer focus;
Strong self-management skills and initiatives;
Driver’s license;
Willingness and readiness to travel 80% of the time.

Assets

Prior sales/customer service experience;
Working knowledge of French.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 17 Jul 2018 20:18:39 -0500
Subject: [Microscopy] viaWWW:Problems with cryo-sectioning

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Email: leandro.lemgrubersoares-at-glasgow.ac.uk Name: Leandro Lemgruber

Organization: University of Glasgow

Title-Subject: [Filtered] Problems with cryo-sectioning

Message: Dear All

lately I'm having problems with cryo-sectioning, more precise picking up
the sections. The sectioning is fine and I'm getting nice sections
(samples embedded in 10% gelatin). However, when I'm picking them up
(with 1:1 methylcellulose/ 2M sucrose) with the loop, the sections don't
seem to attach to the grids (formvar coated ones). I have made new
grids, gelatin, sucrose and methylcellulose; but so far I'm always
ending up with 1-2 sections in one single grid (out of 10 collected grids).
Any ideas or thoughts would be greatly appreciated.

All the best from (currently not so cold) Scotland

Leandro
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From: microscopy.listserver-at-gmail.com
Date: Thu, 19 Jul 2018 20:07:46 -0500
Subject: [Microscopy] viaWWW:EMS Microscopy Academy October Workshops

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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] EMS Microscopy Academy October Workshops

Message: Don't miss out! Sign up for one these October workshops today!


Cryosectioning/Immunogold Workshop
Led by Peter van de Plas, Helmut Gnaegi and Michael Kostrna

Monday - Friday
October 8 - 12, 2018
Hatfield, Pennsylvania, USA

Five days of hands-on training for students, researchers, and
microscopists who want to learn the most up to date theory and practice
in cryosectioning and immunogold labeling Will provide researchers with
the opportunity to learn the theory and practice of the use of
cryosectioning with diamond knives, and immunogold labeling.

https://www.emsmicroscopyacademy.com/product-page/cryoimmuno


Cryo SEM Workshop
Led by Al Coritz and Michael Kostrna

Tuesday - Thursday
October 23 - 25, 2018
Hatfield, Pennsylvania, USA

This course will cover the process of rapid freezing, fracturing,
coating and imaging of a variety of samples. For individuals who are new
to the field of cryo SEM or desire a technical refresh to maintain
current skills or just those that want to see and learn all of the
possibilities of the technology.

https://www.emsmicroscopyacademy.com/product-page/cryosem

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From: microscopy.listserver-at-gmail.com
Date: Fri, 20 Jul 2018 00:56:32 -0500
Subject: [Microscopy] viaWWW: Available Zeiss 10CA TEM spare parts

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Title-Subject: [Filtered] Zeiss 10CA TEM spare parts

Message: All: I have a decommissioned Zeiss 10CA TEM that will be
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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Jul 2018 09:47:34 -0500
Subject: [Microscopy] viaWWW:Opinions and Experience with K-kit for Liquid TEM?

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Email: scottpj-at-vt.edu Name: Philip Scott

Organization: Virginia Tech

Title-Subject: [Filtered] Opinions and Experience with K-kit for Liquid TEM?

Message: Hello!

I am interested in observing some polymer particles (150 nm) in aqueous media without drying for
conventional TEM. I found the Ted Pella K-kit for Liquid TEM which looks perfect for my needs as my
university does not have a liquid cell holder for our TEM's.
Before purchasing this product, I would like some insight into the details and problems (if any)
with using it. For those of you with experience with this product, would you recommend it?

Also what is the resistance of the sealant/glue to organic solvents?

Thank you!
Phil Scott
scottpj-at-vt.edu

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From: bradley.degregorio-at-nrl.navy.mil
Date: Tue, 24 Jul 2018 10:22:47 -0500
Subject: [Microscopy] =?iso-8859-1?Q?Call_for_abstracts_-_Planetary_Science_Session_P038_at_AGU?=

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

Please consider submitting an abstract to the following session at the 2018
AGU Annual Meeting in Washington, D.C., on December 10-14, 2018:
P038: Revealing the origins of our Solar System and its organic compounds
through analysis of meteorites and related planetary materials

Session Description:
Laboratory studies of cosmic dust, meteorites, asteroids, and comets expand
our understanding of the chemical and geophysical origins of our Solar
System. Such studies provide essential constraints for geophysical models of
planetary nebulae, accretion and differentiation of planetesimals, and the
origin and diversity of organic compounds. Furthermore, comprehending the
organic complement of extraterrestrial objects can help distinguish false
positives when searching for indigenous biosignatures. This session invites
papers related to the analysis of meteorites and other similar planetary
materials that advance our understanding of the origin and evolution of our
Solar System and extraterrestrial organic compounds. Topics include
radiative and geochemical processes active in the early solar nebula; the
timing, location, and genetics of planetary accretion and differentiation;
connecting astronomical observations with laboratory data of meteorites,
returned samples, and analog materials; and the origin and evolution of
organic molecules found on asteroids, comets, and their fragments.

Invited Speakers:
Benjamin Weiss (Massachusetts Institute of Technology)
Hiroshi Naraoka (Kyushu University)

Conveners:
Eric Parker (NASA Goddard Space Flight Center)
Bradley De Gregorio (U.S. Naval Research Laboratory)
Emma Bullock (Carnegie Institution of Washington)
Heather Graham (NASA Goddard Space Flight Center)

More information about this session can be found at
https://agu.confex.com/agu/fm18/prelim.cgi/Session/54516
We are also working with American Mineralogist to create a Special Section
for the journal, focusing on research topics presented in our session!
Please consider how your abstract could be expanded into a full length
research paper.
Dont wait too long to submit your abstract. The abstract submission
deadline is August 1st, 23:59 EDT. We look forward to your contribution!

About AGU:
For the first time, the AGU Fall Meeting will be held in Washington, D.C.,
where we will mark the launch AGUs Centennial. A wide variety of events
are being planned that will take advantage of this special location that
will showcase our science to the U.S. and international policy community,
students, and public); leverage the local scientific community, including
events with the Smithsonian, National Academies, and others; and, offer
field trips to view the local geology and research institutes. The Fall
Meeting will also offer more workshops as well as new Tutorial sessions to
help students and researchers learn about new approaches and techniques and
introduce exciting science in other disciplines.
Additional information, including everything you need to know about abstract
submission is here: https://fallmeeting.agu.org/2018/. Please consider
submitting an abstract and/or attending to help show What Science Stands
For and to join in an exciting and informative start to AGUs Centennial.
Submissions received by the early deadline of 25 July have a chance to win a
$100 US gift card.

Bradley T. De Gregorio
Geologist, Code 6366
Materials Science & Technology Division
T (202) 767-6294 F (202) 767-1697

www.nrl.navy.mil



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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Jul 2018 06:39:22 -0500
Subject: [Microscopy] Fwd: Available Jeol 733 Microprobe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Beavers, Roy {rbeavers-at-mail.smu.edu}

After many years we are finally releasing our 1979 vintage Jeol 733 Microprobe.

JEOL JXA-733 Superprobe equipped with four wavelength-dispersive X-ray spectrometers, an Link/Oxford
energy-dispersive system consisting of a detector and analysis software, and Advanced MicroBeam
automation.

If you have an interest is this system please contact me offline at Email below.


Roy Beavers
Southern Methodist University
Department of Earth Sciences
3225 Daniel Ave
Dallas TX 75205
Voice: 214-768-2756
Email: rbeavers-at-smu.edu


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Jul 2018 06:41:09 -0500
Subject: [Microscopy] viaWWW:Biorad Lasersharp 2000 and Win 7.

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

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Email: ugez323-at-yahoo.co.uk Name: Jonathan

Organization: UCL

Title-Subject: [Filtered] Biorad Lasersharp 2000 and Win 7.

Message: Hi

We are trying to keep our Bird confocal running and wondered if the software could run on Windows 7?
Has anyone managed to do this?

It seems it might be possible. We gave it a go and on starting the software the confocal head clicks
so it seems to be talking to it, but the software then comes up with an error (.ocx file not
registered). Tried to register it manually but it won't do it.

Any pointers appreciated!

Thanks

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From: hsia627-at-hotmail.com
Date: Wed, 25 Jul 2018 09:13:22 -0500
Subject: [Microscopy] Pre-M&M Open House at U Maryland Baltimore EM Facility

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

I would like to inform you that Electron Microscopy Core Imaging Facility (EMCIF) of University of Maryland Baltimore (UMB) will host a pre-M&M open house and instrument demo on August 4th and 5th (Sat & Sun) from 10 AM to 4 PM each day. All our sample preparation equipment, scopes, and some vendor supplied instrument will be available for try out. The purpose of this open house is to provide an opportunity for the general public to tour a multifunctional EM Facility and for EM professionals to test small equipment designed to enhance workflow and efficiency in modern EM Facilities. In particular, these include the PELCO BioWave Pro+ from Ted Pella; GloQubeTM, Lynx II tissue processor from EMS; ASP1000 automated EM sample processor from Microscopy Innovations; and EM GP from Leica Microsystems, etc.

UMB is located in downtown Baltimore, within walking distance from the Baltimore Convention Center, Camden yard, Edgar Allen Poes Cemetery. There will be light refreshment served all day, so please drop in while you are touring Inner Harbor and Baltimore City. Walk-ins are welcome. Kids and Family members are also welcome. You have the option to signup ahead to reserve your space for pre-scheduled instrument demonstration.

For more information and links to signup specific instrument demo, please visit:
http://www.dental.umaryland.edu/core-imaging/open-house--instrument-demo/

We look forward to seeing you in Baltimore.

Sincerely,
Ru-ching Hsia
rhsia-at-umaryland.edu
Director, Electron Microscopy Core Imaging Facility
University of Maryland, Baltimore
Tel: 410-706 7992
http://www.dental.umaryland.edu/Core-imaging
Rm 696B, Howard Hall, 660 W. Redwood St. Baltimore, MD 21201



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8, 63 -- Subject: Pre-M&M Open House at U Maryland Baltimore EM Facility
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From: ech-at-uvic.ca
Date: Wed, 25 Jul 2018 14:38:44 -0500
Subject: [Microscopy] Reminder: Fun in Baltimore: The Great LEGO Microscope Competition

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Make your smart phone into a microscope using LEGO. M&M 2018 Baltimore

Bring your designs to the Outreach Booth at the Megabooth in the Exhibitors’ Hall asap.
Elaine
Ps if anyone has made instruments of SEM, TEM etc out of LEGO we would love to see them too.

Dr. Elaine C. Humphrey
Advanced Microscopy Facility
Bob Wright Science Centre A015
University of Victoria, Canada

Lab: 250-853-3968
cell: 250-886-2068
website: http://www.stehm.uvic.ca




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From: zaluzec-at-microscopy.com
Date: Sun, 29 Jul 2018 10:06:07 -0500
Subject: [Microscopy] viaWWW:Hitachi S-570 SEM small tools needed

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Email: mbrazil-at-vergason.com Name: Michael Brazil

Organization: Vergason Technology, Inc.

Title-Subject: [Filtered] Hitachi S-570 SEM small tools needed

Message: I have recently taken responsibility for a 34-year-old Hitachi S-570 SEM, tungsten
filament. It did not come with filament alignment jig, height setting scale, or tools for changing
apertures. If anyone has these rattling around, I'd be happy to purchase. If you need to keep them,
I'd be very grateful for close-up pictures to allow me to replicate them. Hitachi may eventually
offer to make new ones, but I suspect the cost will be prohibitive.
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From: microscopy.listserver-at-gmail.com
Date: Mon, 30 Jul 2018 19:37:06 -0500
Subject: [Microscopy] viaWWW:Macroviewer/Light Box for Donation

Contents Retrieved from Microscopy Listserver Archives
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Title-Subject: [Filtered] Macroviewer/Light Box for Donation

Message: Macroviewer/Lightbox for donation
Includes overhead lamps and movable stage
Available for pickup in Wisconsin or shipping at donee's expense. Pictures available upon request



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From: microscopy.listserver-at-gmail.com
Date: Mon, 30 Jul 2018 19:37:50 -0500
Subject: [Microscopy] viaWWW:Inverted Microscope Stand for Donation

Contents Retrieved from Microscopy Listserver Archives
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Email: Diane.M.Curley-at-KCC.com Name: Diane M, Curley, PhD

Organization: Kimberly-Clark

Title-Subject: [Filtered] Inverted Microscope Stand for Donation

Message: Olympus IX70 inverted microscope stand for donation
Great for customization
Available for pickup in Wisconsin or can be shipped at donee's expense.

Pictures available upon request!


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From: mbrazil-at-vergason.com
Date: Tue, 31 Jul 2018 08:22:25 -0500
Subject: [Microscopy] Looking for small tools to support Hitachi S-570 SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have recently taken over a Hitachi S-570 SEM, built in 1984. It lacks some of the small tools. I would like to purchase them. Hitachi does not stock.
If you have some left lying around that you no longer need, great.
If you have some that you need, would you be willing to send me photos with a scale in the image? It would help me recreate them.
The particular items of interest are:
Hitachi Part Number Description
531-1470 Filament Exchange Jig
538-0636 Scale (used for setting height)
538-1437 Tool for objective aperture set
538-1438 Tool for objective aperture exchange

Thank you,
Michael Brazil
Senior Scientist
Vergason Technologies, Inc.
166 Route 224
Van Etten, NY 14889
(607) 589-3914 (office)
mbrazil-at-vergason.com
www.vergason.com
www.linkedin.com/in/mbrazil/en



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4, 66 -- From: Michael Brazil {mbrazil-at-vergason.com}
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4, 66 -- Subject: Looking for small tools to support Hitachi S-570 SEM
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From: holpc-at-firstenergycorp.com
Date: Tue, 31 Jul 2018 10:03:58 -0500
Subject: [Microscopy] EDS calibration/verification

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I would appreciate feedback from the group regarding your practices and policies of the calibration of your EDS systems.

A few topics of particular interest are:

How often do you perform the calibration routine?
Are you satisfied that the automatic calibration is sufficient?
Can you (and do you) collect "As found" as well as "As left" FWHM energy levels for any elements?

Thanks in advance for any and all thoughts!

Chris Holp
FirstEnergy
BETA Labs
6670 Beta Drive
Mayfield Village OH 44143

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From: steven.spurgeon-at-pnnl.gov
Date: October 8-10, 2018
Subject: [Microscopy] Early Registration Deadline Today for NexTEM Workshop at PNNL

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

The early registration deadline for the NexTEM workshop at PNNL is today! Please read below for more details and visit https://pnnl.cvent.com/nextem to register. We have a stellar lineup of invited speakers, sessions, and social events and we hope you are able to attend.

----------------

Next-Generation Transmission Electron Microscopy (NexTEM) Workshop
Beyond Current Limits of Resolution, Environments, and Data Analysis


Overview

Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.

Topics

High-Resolution Imaging and Spectroscopy
– New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems.
– Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision.
– Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces.
– Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples.
– Novel detectors for improved high-speed imaging and spectroscopy.

In Situ / Operando Microscopy and Extreme Environments
– Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids.
– Investigation of materials under stimulus across a range of sample environments and temperatures.

Computational Methods for Data Analysis
– Software to improve data collection quality, accuracy, and acquisition rates.
– Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis.
– Machine learning approaches for high-throughput data processing and feature detection.
– Image simulation tools to aid the interpretation of experimental images and spectroscopy.

Confirmed Invited Speakers
– Ondrej Krivanek, Nion Co.
– Amanda Petford-Long, Argonne National Laboratory
– Sergei Kalinin, Oak Ridge National Laboratory
– Renu Sharma, National Institute of Standards and Technology
– Rafal Dunin-Borkowski, Forschungszentrum Jülich
– Robert Klie, University of Illinois–Chicago
– Khalid Hattar, Sandia National Laboratories
– Marc DeGraef, Carnegie Mellon University
– James LeBeau, North Carolina State University
– Haimei Zheng, Lawrence-Berkeley National Laboratory
– Colin Ophus, Molecular Foundry
– Juan Carlos Idrobo, Oak Ridge National Laboratory
– Rama Vasudevan, Oak Ridge National Laboratory
– R. Lee Penn, University of Minnesota
– Paolo Longo, Gatan
– Lewys Jones, Trinity College–Dublin

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com
www.pnnl.gov



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From: tbargar-at-unmc.edu
Date: Wed, 1 Aug 2018 13:09:06 -0500
Subject: [Microscopy] anybody doing quantitative TEM using Stereology

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,
I'll be attending the M&M meeting in Baltimore. I would like to meet with anyone who is doing quantitative TEM using stereology.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347



The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: sergei2-at-ornl.gov
Date: Thu, 2 Aug 2018 08:24:14 -0500
Subject: [Microscopy] November 1-2: Atom-by-atom fabrication by electron beams and scanning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues
We are now planning the workshop “Atom by atom fabrication
via electron beams and scanning probes”, to be held at Oak Ridge
National Laboratory on November 1-2 this coming fall. This workshop will
bring together
experts in the emerging field of atomically resolved electron beam
matter manipulation
and scanning probe atomic fabrication, to highlight the
recent advances and opportunities and serve as a much-needed seed to
establish its rapid growth. It will provide a forum to present recent
achievements in scanning probe and electron beam manipulation, novel
opportunities for instrumental development enabled by the availability
of high speed data analytic tools and machine learning, integration of
atomic-scale device engineering into semiconductor workflows, and
opportunities for quantum information systems and quantum computing.
The confirmed plenary speakers at the workshop are Toma Susi (U
Vienna) and
J. Randall (Zyvex), with a number of invited talks by the leading
experts in the field.
Additional invited and contributed talks will be selected from submitted
abstracts.
If interested - mark the time in your calendars!
Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, Foresight Institute, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Aug 2018 07:00:36 -0500
Subject: [Microscopy] viaWWW: 2 Postdoc positions available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




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Title-Subject: [Filtered] Postdoc positions available

Message: Two postdoc positions are available at Monash University in Melbourne Australia.

1) Research Fellow – Electron Microscopy of Nanoparticle Systems

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From: sergei2-at-ornl.gov
Date: Mon, 13 Aug 2018 09:21:12 -0500
Subject: [Microscopy] PyCroscopy workshop - streamed and recorded

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

We are delighted to provide information on two seminars run from Oak Ridge National Laboratory as part of the Center for Nanophase Materials Sciences User Meeting 2018 Workshops. Both workshops will focus on using python and available scientific packages to analyze a range of imaging and spectral datasets. The workshop details and timings are below:


1. Monday, August 13th 2018: Imaging and Spectral Data Analysis in Python

Full details are available on our github page,https://github.com/pycroscopy/pyUSID_Tutorial

Topics covered on Monday: Basic python, Jupyter notebooks, the pyUSID data model, basic spectral and image processing, rounding out with talks.

It will be streamed* on Bluejeans via the following link:https://bluejeans.com/782808739


1. Wednesday, August 13th 2018: Machine Learning for Materials Science with Python

Topics covered include Linear and Nonlinear Unmixing methods for spectral data, Basic classification (supervised and unsupervised), and an introduction to deep learning for microscopy datasets.

It will be streamed* via the following link: bluejeans.com/678595624 {http://bluejeans.com/678595624}

*Note that due to streaming bandwidth, there are a limited number of viewer slots. We apologize in advance if the link is non-functioning due to demand. But, both workshops will be recorded and posted at a later date.

Kind Regards,
Rama Vasudevan on behalf of the organizing team
Center for Nanophase Materials Sciences,
Oak Ridge National Laboratory


--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Mon, 13 Aug 2018 10:13:28 -0500
Subject: [Microscopy] viaWWW:EMS Microscopy Academy Workshops. Fall classes

Contents Retrieved from Microscopy Listserver Archives
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Email: stacie-at-ems-secure.com Name: Stacie Kirsch

Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] EMS Microscopy Academy Workshops. Fall classes starting soon.

Message: Don't miss these exciting workshops coming this fall. Feel free to reach out to me with any
questions.
- Stacie Kirsch

Cryosectioning/Immunogold Workshop
Led by Peter van de Plas, Helmut Gnaegi and Michael Kostrna Monday - Friday
October 8 - 12, 2018
Hatfield, Pennsylvania, USA
Five days of hands-on training for students, researchers, and microscopists who want to learn the
most up to date theory and practice in cryosectioning and immunogold labeling Will provide
researchers with the opportunity to learn the theory and practice of the use of cryosectioning with
diamond knives, and immunogold labeling.
Sign up at https://www.emsmicroscopyacademy.com/product-page/cryoimmuno

Cryo SEM Workshop
Led by Al Coritz and Michael Kostrna Tuesday - Thursday
October 23 - 25, 2018
Hatfield, Pennsylvania, USA
This course will cover the process of rapid freezing, fracturing, coating and imaging of a variety
of samples. For individuals who are new to the field of cryo SEM or desire a technical refresh to
maintain current skills or just those that want to see and learn all of the possibilities of the
technology.
Sign up at https://www.emsmicroscopyacademy.com/product-page/cryosem

Biological TEM Workshop
Led by Al Coritz and Michael Kostrna Tuesday - Thursday
November 6 - 8, 2018
Hatfield, Pennsylvania, USA
Theory and practical preparation of buffers, fixatives, and other solutions required for chemical
processing of biological samples for TEM. For individuals who are, or will be, responsible for
chemical processing and sectioning of biological samples for TEM examination.
Sign up at https://www.emsmicroscopyacademy.com/product-page/biotem

Biological SEM Workshop
Led by Al Coritz and Michael Kostrna Tuesday - Thursday
November 13 - 15, 2018
Hatfield, Pennsylvania, USA
This course will introduce participants to methods of sample preparation and SEM parameters and
operation needed for accurate analysis. For individuals who currently perform or will be responsible
for the preparation of samples and/or operation of the SEM in a research, academic, or industrial
setting.
Sign up at https://www.emsmicroscopyacademy.com/product-page/biosem

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From: microscopy.listserver-at-gmail.com
Date: Tue, 14 Aug 2018 07:12:09 -0500
Subject: [Microscopy] viaWWW :Biological TEM Workshop at UGa!

Contents Retrieved from Microscopy Listserver Archives
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop again!

Message: October 2018
This intensive, three-day workshop provides a practical and basic theoretical introduction to the
Transmission Electron Microscope and biological sample preparation techniques. Each day will consist
of lecture, discussion and hands-on training led by GEM staff.
What: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment. When: Wednesday through Friday,
October 24-26, 2018, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: Oct 17, 2018


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From: sergei2-at-ornl.gov
Date: Wed, 15 Aug 2018 08:01:37 -0500
Subject: [Microscopy] Updated link - PyCroscopy workshop livestream

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

Thank you for interest in the Machine learning for materials science
with python workshop, part of the CNMS User Meeting 2018.
The bluejeans link is https://ornl.bluejeans.com/1766282540

You may find the agenda and course materials available on github here:

https://github.com/pycroscopy/CNMS_ML_in_MS_Workshop_2018

Kind Regards,

Rama

--

Dr. Rama K. Vasudevan

/R&D Associate/

Center for Nanophase Materials Sciences

Oak Ridge National Laboratory

Oak Ridge, TN 37831, USA

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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17, 37 -- Subject: Updated link - PyCroscopy workshop livestream
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From: microscopy.listserver-at-gmail.com
Date: Thu, 16 Aug 2018 08:31:09 -0500
Subject: [Microscopy] viaWWW:GWNIC CLEM workshop Sept 24-28 2018

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] GWNIC CLEM workshop Sept 24-28 2018

Message: Good morning,

The George Washington University Nanofabrication and Imaging Center would like to announce it's fall
workshop.

Please join us Sept 24-28, 2018 in Washington DC for a week of intensive CLEM presentations,
demonstrations and discussions. Please follow the link below for more information.


https://nic.gwu.edu/clem-workshop-2018

Chris Brantner
GWNIC staff
gwnic-at-gwu.edu

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From: parishcm-at-ornl.gov
Date: Thu, 16 Aug 2018 11:57:29 -0500
Subject: [Microscopy] EBSD of organics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Has anyone ever tried (or seen literature on) EBSD or transmission Kikuchi diffraction of organic crystals? Ever has any success? I would be interested in any experiences you could share.

Vendors, please contact me off list about your highest-sensitivity EBSD and especially tKD systems.

Thanks
Chad

--------------------------------
Chad M. Parish, Ph.D.
Research and Development Staff Member
Radiation Effects and Microstructural Analysis Team
Nuclear Materials Science and Technology Group
Materials Science and Technology Division
Oak Ridge National Laboratory
Phone: 1 865 574 0092
Email: parishcm-at-ornl.gov



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From: j.sharp-at-sheffield.ac.uk
Date: Mon, 20 Aug 2018 11:19:59 -0500
Subject: [Microscopy] Struers secotom?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everybody,

Long shot... we have an abrasion saw - a Struers Secotom 50 - and
somebody has lost the little plastic lid that goes on the hole you use
for emptying out the cooling water tank, so we can't fill the tank up
and run the saw. It is a little round plastic lid, 20mm in diameter,
like a bottle top.

Does anybody out there maybe have a dead Struers Secotom 50 that has
that lid still? Or a Struers anything with a lid like it, in case they
used the same one?

We have looked *everywhere*. We have asked if they will sell us a lid
and the answer was "it's complicated". I am measuring every bottle lid
I see just in case it fits...

Sorry this is not entirely a microscopy question, but it is delaying a
lot of microscopy....

Yours hopefully

Jo Sharp, postdoc, Sheffield

==============================Original Headers==============================
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From: John.Mardinly-at-asu.edu
Date: Mon, 20 Aug 2018 12:31:57 -0500
Subject: [Microscopy] Re: Struers secotom?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It’s not complicated: Struers customer service is…missing in action. Are they going out of business?

} On Aug 20, 2018, at 9:25 AM, j.sharp-at-sheffield.ac.uk wrote:
}
}
}
}
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}
} Hi everybody,
}
} Long shot... we have an abrasion saw - a Struers Secotom 50 - and
} somebody has lost the little plastic lid that goes on the hole you use
} for emptying out the cooling water tank, so we can't fill the tank up
} and run the saw. It is a little round plastic lid, 20mm in diameter,
} like a bottle top.
}
} Does anybody out there maybe have a dead Struers Secotom 50 that has
} that lid still? Or a Struers anything with a lid like it, in case they
} used the same one?
}
} We have looked *everywhere*. We have asked if they will sell us a lid
} and the answer was "it's complicated". I am measuring every bottle lid
} I see just in case it fits...
}
} Sorry this is not entirely a microscopy question, but it is delaying a
} lot of microscopy....
}
} Yours hopefully
}
} Jo Sharp, postdoc, Sheffield
}
} ==============================Original Headers==============================
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} 7, 42 -- Date: Mon, 20 Aug 2018 15:18:03 +0100
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4, 104 -- Subject: Re: [Microscopy] Struers secotom?
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From: j.sharp-at-sheffield.ac.uk
Date: Tue, 21 Aug 2018 05:07:01 -0500
Subject: [Microscopy] Re: Struers secotom?

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Well they came to fix our Tenupol a few weeks ago, so they're not
completely missing, they just can't instantly send us this one thing.

Jo

On 20 August 2018 at 18:30, John Mardinly {John.Mardinly-at-asu.edu} wrote:
} It’s not complicated: Struers customer service is…missing in action. Are they going out of business?
}
} } On Aug 20, 2018, at 9:25 AM, j.sharp-at-sheffield.ac.uk wrote:
} }
} }
} }
} }
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} }
} } Hi everybody,
} }
} } Long shot... we have an abrasion saw - a Struers Secotom 50 - and
} } somebody has lost the little plastic lid that goes on the hole you use
} } for emptying out the cooling water tank, so we can't fill the tank up
} } and run the saw. It is a little round plastic lid, 20mm in diameter,
} } like a bottle top.
} }
} } Does anybody out there maybe have a dead Struers Secotom 50 that has
} } that lid still? Or a Struers anything with a lid like it, in case they
} } used the same one?
} }
} } We have looked *everywhere*. We have asked if they will sell us a lid
} } and the answer was "it's complicated". I am measuring every bottle lid
} } I see just in case it fits...
} }
} } Sorry this is not entirely a microscopy question, but it is delaying a
} } lot of microscopy....
} }
} } Yours hopefully
} }
} } Jo Sharp, postdoc, Sheffield
} }
} } ==============================Original Headers==============================
} } 7, 42 -- From j.sharp-at-sheffield.ac.uk Mon Aug 20 11:19:46 2018
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} } 7, 42 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk}
} } 7, 42 -- Date: Mon, 20 Aug 2018 15:18:03 +0100
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} } 7, 42 -- Subject: Struers secotom?
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4, 49 -- From: Joanne Sharp {j.sharp-at-sheffield.ac.uk}
4, 49 -- Date: Tue, 21 Aug 2018 11:04:58 +0100
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4, 49 -- Subject: Re: [Microscopy] Struers secotom?
4, 49 -- To: John Mardinly {John.Mardinly-at-asu.edu}
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From: tomas.hrncir-at-tescan.com
Date: Tue, 21 Aug 2018 06:07:04 -0500
Subject: [Microscopy] Re: Struers secotom?

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Jo,
maybe you could replace it (at least temporarily, until they will manage to send you the part) by something hand-made from soft rubber or cork? 20 mm in diameter, it looks like wine or champagne cork :-).
Good luck,
Tomas
________________________________________
X-from: j.sharp-at-sheffield.ac.uk {j.sharp-at-sheffield.ac.uk}
Sent: 21 August 2018 12:08
To: Tom Hrn

Well they came to fix our Tenupol a few weeks ago, so they're not
completely missing, they just can't instantly send us this one thing.

Jo

On 20 August 2018 at 18:30, John Mardinly {John.Mardinly-at-asu.edu} wrote:
} Its not complicated: Struers customer service ismissing in action. Are they going out of business?
}
} } On Aug 20, 2018, at 9:25 AM, j.sharp-at-sheffield.ac.uk wrote:
} }
} }
} }
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} }
} } Hi everybody,
} }
} } Long shot... we have an abrasion saw - a Struers Secotom 50 - and
} } somebody has lost the little plastic lid that goes on the hole you use
} } for emptying out the cooling water tank, so we can't fill the tank up
} } and run the saw. It is a little round plastic lid, 20mm in diameter,
} } like a bottle top.
} }
} } Does anybody out there maybe have a dead Struers Secotom 50 that has
} } that lid still? Or a Struers anything with a lid like it, in case they
} } used the same one?
} }
} } We have looked *everywhere*. We have asked if they will sell us a lid
} } and the answer was "it's complicated". I am measuring every bottle lid
} } I see just in case it fits...
} }
} } Sorry this is not entirely a microscopy question, but it is delaying a
} } lot of microscopy....
} }
} } Yours hopefully
} }
} } Jo Sharp, postdoc, Sheffield
} }
} } ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Sat, 25 Aug 2018 06:24:12 -0500
Subject: [Microscopy] viaWWW: CDC - Seeking a life sciences electron microscopist

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Email: CGoldsmith-at-cdc.gov Name: Cynthia Goldsmith

Organization: Centers for Disease Control and Prevention (CDC)

Title-Subject: [Filtered] Seeking a life sciences electron microscopist

Message: The Infectious Diseases Pathology Branch, National Center for Emerging and Zoonotic
Diseases, Centers for Disease Control and Prevention (CDC) is seeking a person with transmission
electron microscopy (TEM) experience. Our laboratory uses thin section and negative stain techniques
to diagnose and characterize pathogens of public health importance, including viral, bacterial, and
parasitic diseases. The EM lab has been instrumental during outbreaks of SARS coronavirus,
poxviruses, Zika virus, Nipah virus, transplant-associated viral transmissions, and the hemorrhagic
fever viruses. Projects have included the EM description of emerging viruses, characterization of
humanized mice infected with BSL-4 viruses, quality control of virus-like particles to be used in
immunoassays, and examination by TEM of formalin-fixed paraffin-embedded specimens that have been
submitted to the branch.
CDC serves as the federal government agency for public health concerns, and is a reference center
for US state health departments and for the World Health Organization. The Infectious Diseases
Pathology Branch consists of pathologists, histotechnologists, molecular biologists, a
microbiologist, and an electron microscopist who work together to obtain a diagnosis by using
tissues and tissue culture isolates. To further the characterization of infectious diseases, the EM
lab also uses immunogold labeling and in situ hybridization techniques. Equipment available includes
two FEI/Thermo Fisher 120 kV transmission electron microscopes each with an AMT BioSprint 16 camera,
a Pelco BioWave Pro+ microwave for tissue processing, and two Leica ultramicrotomes (UC7 and UC UCT).

The ideal candidate would have a minimum of a Bachelor's degree in biology or related discipline and
some experience with biological TEM techniques, including thin section and negative stain methods.
Capability with TEM instrument usage plus knowledge of microbiology and/or molecular biology would
be advantageous. The applicant should be a US citizen or a permanent resident. Compensation will be
commensurate with education and experience. CDC is an equal opportunity employer, and is a
smoke-free environment.

If you have any questions, please contact Cynthia Goldsmith at 404-639-3306 (CGoldsmith-at-cdc.gov).

Please send a letter describing your experience and interests, along with your resume, to Sherif
Zaki, Chief, Infectious Diseases Pathology Branch (SZaki-at-cdc.gov), Roose Martines
(RBrasilMartines-at-cdc.gov) and Cynthia Goldsmith (CGoldsmith-at-cdc.gov).


Centers for Disease Control and Prevention (CDC)
1600 Clifton Road NE
Atlanta, GA 30329




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From: microscopy.listserver-at-gmail.com
Date: Sat, 25 Aug 2018 06:32:39 -0500
Subject: [Microscopy] viaWWW:Postdoc position available

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Email: yuziliu-at-anl.gov Name: Yuzi Liu

Organization: Argonne National Laboratory

Title-Subject: [Filtered] Postdoc position available

Message: We are seeking a self-motivated and creative postdoctoral fellow to conduct experimental
research in the areas of cathode materials for sodium-ion batteries. In this role, you will use your
experience and education in the fields related to solid state synthesis of electrode materials,
electrochemistry, and advanced characterization and data analysis (e.g.,analytical TEM, XRD,
Rieltveld refinement, synchrotron spectroscopy) to support ongoing efforts and to develop and lead
new efforts.
This project is a close collaboration between the Electrochemical Energy Materials Lab at Boise
State University (clairexiong-at-boisestate.edu) and Argonne National Lab. Travels to Argonne annually
are expected for this position. Hands on experience and data analysis on (S)TEM are highly desired.
Thank you!

Yuzi Liu

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From: steven.spurgeon-at-pnnl.gov
Date: October 8-10, 2018
Subject: [Microscopy] =?utf-8?B?RmluYWwgUmVtaW5kZXIgQWJvdXQgTmV4VEVNIFdvcmtzaG9wIGF0IFBOTkwg?=

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,

This is a final reminder that registration for the NexTEM workshop at PNNL closes this Friday, August 31st. We have over 40 registrants already, including distinguished speakers from around the world, and we hope you'll be able to join us. Please read below for more details and visit https://pnnl.cvent.com/nextem to sign up today!

----------------

Next-Generation Transmission Electron Microscopy (NexTEM) Workshop
Beyond Current Limits of Resolution, Environments, and Data Analysis


Overview

Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.

Topics

High-Resolution Imaging and Spectroscopy
– New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems.
– Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision.
– Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces.
– Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples.
– Novel detectors for improved high-speed imaging and spectroscopy.

In Situ / Operando Microscopy and Extreme Environments
– Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids.
– Investigation of materials under stimulus across a range of sample environments and temperatures.

Computational Methods for Data Analysis
– Software to improve data collection quality, accuracy, and acquisition rates.
– Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis.
– Machine learning approaches for high-throughput data processing and feature detection.
– Image simulation tools to aid the interpretation of experimental images and spectroscopy.

Confirmed Invited Speakers
– Ondrej Krivanek, Nion Co.
– Amanda Petford-Long, Argonne National Laboratory
– Sergei Kalinin, Oak Ridge National Laboratory
– Renu Sharma, National Institute of Standards and Technology
– Rafal Dunin-Borkowski, Forschungszentrum Jülich
– Robert Klie, University of Illinois–Chicago
– Khalid Hattar, Sandia National Laboratories
– Marc DeGraef, Carnegie Mellon University
– James LeBeau, North Carolina State University
– Haimei Zheng, Lawrence-Berkeley National Laboratory
– Colin Ophus, Molecular Foundry
– Juan Carlos Idrobo, Oak Ridge National Laboratory
– Rama Vasudevan, Oak Ridge National Laboratory
– R. Lee Penn, University of Minnesota
– Paolo Longo, Gatan
– Lewys Jones, Trinity College–Dublin

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com
www.pnnl.gov



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From: tbargar-at-unmc.edu
Date: Mon, 27 Aug 2018 16:37:38 -0500
Subject: [Microscopy] Would like to talk to experienced users of High Pressure Freezing and

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Mon, 27 Aug 2018 16:37:38 -0500

Dear Listers,

I would like to get in contact with any experienced users of the Leica High Pressure Freezer and Freeze Substitution instruments. Please respond privately with your contact information as I would like to call and explain what I am experiencing. Easier to explain directly than to try and write it out.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: sergei2-at-ornl.gov
Date: Thu, 30 Aug 2018 11:56:58 -0500
Subject: [Microscopy] Registration open - ORNL workshop on atom by atom fabrication via

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues If you are interested in applying STEM not only to
visualize matter, but also to control it, move atom, and build devices
by electron beam - the registration is now open for the inaugural
workshop “Atom by atom fabrication via electron beams and scanning
probes”, to be held at Oak Ridge National Laboratory on November 1,2.
This workshop will bring together experts in scanning probe atomic
fabrication and the rapidly emerging field of atomically resolved
electron beam matter manipulation, to highlight the recent advances and
opportunities and serve as a much-needed seed to establish its rapid
growth. It will provide a forum to present recent achievements in
electron beam manipulation, novel opportunities for instrumental
development enabled by the availability of high speed data analytic
tools and machine learning, integration of atomic-scale device
engineering into semiconductor workflows, an opportunities for quantum
information systems. The confirmed plenary speakers include Toma Susi
(U. Vienna) and John Randall (Zyvex), and invited speakers (partial and
tentative list) include Joe Lyding (Illinois), David Forrest (DOE), Dirk
Englund (MIT), Prineha Narang (Harvard), Quentin Ramasse (SuperSTEM),
Marija Drndic (U Penn), A. Lupini (ORNL).

So, please spread the word, and register!

https://utconferences.eventsair.com/workshop-on-atom-by-atom-fabrication-via-electron-beams-and-scanning-probes/register

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, Foresight Institute, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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7, 38 -- Subject: Registration open - ORNL workshop on atom by atom fabrication via
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From: microscopy.listserver-at-gmail.com
Date: Fri, 31 Aug 2018 07:44:36 -0500
Subject: [Microscopy] viaWWW:Reichert CS auto Freeze unit

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-------- Forwarded Message --------

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Email: lefty-at-gene.com Name: Dave Lawrence

Organization: Genentech

Title-Subject: [Filtered] Reichert CS auto Freeze unit

Message: Hello, does anyone have any info on a Type 702101 sn 400660 Reichert auto Freeze unit?
It's kind of very old and we have a lab that wants to use this for some experiments.

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From: nikd-at-vsl.cua.edu
Date: Fri, 31 Aug 2018 15:00:51 -0500
Subject: [Microscopy] Ladd evaporator

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Our SEM lab is considering a new carbon vacuum evaporator to replace our
Veeco.

We've looked at a number of makes and models, but the one that seems to
offer the best price for our purposes is the Ladd Classic Floor model.

Has anyone had any experience with this?

Cheers,
Nik Deems - SEM Lab Tech
Vitreous State Laboratory - Catholic University of America



==============================Original Headers==============================
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7, 28 -- Date: Fri, 31 Aug 2018 16:00:02 -0400
7, 28 -- Subject: Ladd evaporator
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From: steven.spurgeon-at-pnnl.gov
Date: Sat, 1 Sep 2018 21:56:10 -0500
Subject: [Microscopy] Postdoc Position in Analytical STEM and APT of Quantum Materials

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School October 2018

Message: EELS & EFTEM Analysis Training School October 2018

Location: Gatan R&D Headquarters, Pleasanton, CA

Dear Colleagues,

I am looking for a postdoc in analytical STEM and APT of quantum materials systems. If you or anyone you know is interested, please see the details below and apply at: https://pnnl.jibeapply.com/jobs/308260?lang=en-us

---------------------------------------------------------------------------------
Post Doc Research Associate - Quantum Materials Microscopy and Tomography
Locations: RICHLAND, WA
Job ID: 308260
Full/Part Time: Full-Time
Regular/Temporary: Temporary

Job Description
The successful candidate will utilize aberration-corrected scanning transmission electron microscopy (STEM) and atom probe tomography (APT) to examine III/V semiconductors for quantum materials systems. Approximately 50% of the candidate’s time will be spent preparing TEM samples, conducting atomic-resolution imaging and chemical analysis using STEM, energy-dispersive X-ray spectroscopy (EDS), and electron energy loss spectroscopy (EELS). The other 50% of the candidate’s time will be spent preparing APT specimens, APT experimental data collection, reconstruction, and detailed analysis, with the goal of generating complementary datasets. The candidate will be encouraged to propose, plan, and conduct their own related research. The candidate is also expected to have expert level proficiency in focused ion beam (FIB)-based site specific lift-out preparation of TEM and APT samples.

Equal Employment Opportunity
Battelle Memorial Institute (BMI) at Pacific Northwest National Laboratory (PNNL) is an Affirmative Action/Equal Opportunity Employer and supports diversity in the workplace. All employment decisions are made without regard to race, color, religion, sex, national origin, age, disability, veteran status, marital or family status, sexual orientation, gender identity, or genetic information. All BMI staff must be able to demonstrate the legal right to work in the United States. BMI is an E-Verify employer. Learn more at jobs.pnnl.gov.

Minimum Qualifications
Candidates must have received a PhD within the past five years (60 months) or within the next 8 months from an accredited college or university.

Preferred Qualifications
• At least 3 years of experience in Scanning / Transmission Electron Microscopy.
• Strong oral and written communication skills.
• Ph.D. in Materials Science and/or Engineering or Physics.
• Candidates must have a strong background in their major field of study, the ability to work independently with little supervision, and an interest in working with others as part of an interdisciplinary team of researchers addressing challenges with national and international impact.
• Experience in TEM and/or APT sample preparation using FIB and/or mechanical polishing techniques.
• Experience in conducting atomic-resolution STEM imaging, EDS, and EELS spectroscopy of complex oxides or semiconductors.
• Experience in laser assisted and voltage APT experimental procedures, reconstruction of data using IVAS software and detailed APT data analysis.
• Experience with direct correlation of APT and STEM.
• Specific expertise on APT analysis of semiconductor devices and understanding of strategies to correct reconstruction artifacts and increase analysis yield.
• Familiarity with image simulation (multislice / Bloch wave) techniques for the interpretation of STEM images and spectroscopic data.
• Familiarity with image analysis tools (ImageJ, Matlab) and Python programming skills.

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com
www.pnnl.gov



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11, 39 -- Subject: Postdoc Position in Analytical STEM and APT of Quantum Materials
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From: sergei2-at-ornl.gov
Date: Thu, 6 Sep 2018 12:26:43 -0500
Subject: [Microscopy] Postdoctoral opportunity - ORNL

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues

Currently, Andy Lupini and I have several postdoctoral positions open in
the area of high resolution STEM-EELS of quantum materials. The
perspective candidate will have access to the full spectrum of STEM
tools at ORNL (including Nion MAC-STEM, U200, etc), and will have an
opportunity to collaborate with broad range of theorists and materials
synthesis groups at ORNL and worldwide, and also will have an
opportunity to work with big data/machine learning groups at ORNL.
Please contact me and Andy directly (sergei2-at-ornl.gov and arl1000-at-ornl.gov).

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Fri, 7 Sep 2018 01:49:02 -0500
Subject: [Microscopy] viaWWW:GWNIC CLEM workshop Sept 24-28 2018

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: George Washington University

Title-Subject: [Filtered] GWNIC CLEM workshop Sept 24-28 2018

Message: Good afternoon,

The George Washington University Nanofabrication and Imaging Center
would like to announce it's fall workshop.

Please join us Sept 24-28, 2018 in Washington DC for a week of intensive
CLEM presentations, demonstrations and discussions. Please follow the
link below for more information.


https://nic.gwu.edu/clem-workshop-2018

Chris Brantner
GWNIC staff
gwnic-at-gwu.edu

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From: microscopy.listserver-at-gmail.com
Date: Fri, 7 Sep 2018 16:45:20 -0500
Subject: [Microscopy] Fwd: magnification on a side-mounted camera

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-------- Forwarded Message --------

X-from: Stephane Nizet {nizets2-at-yahoo.com}
Reply-To: Stephane Nizet {nizets2-at-yahoo.com}


Dear colleagues,

I am quite sure that I read a discussion about this before but I cannot
find it anymore so I'm asking again, sorry for that.
We have a side-mounted camera (Megaview III) on a tecnai G20 microscope.
The magnification given by the microscope corresponds to the image on
the phosphor screen.
But since the camera is not in the same plane and the beam is not
parallel, the magnification on the camera is different.

Of course I calibrate the camera so my measurements on pictures taken by
the camera are (more or less) right, but how can I know exactly at which
magnification the picture has been taken?
And, even more importantly, does it matter?
Since the digital pictures can be printed at (almost) any size, this
would mean that I end up with different magnifications, am I right?

If the microscope tells me that I used a 40 000x magnification, what
should I write in my digital picture if I want to write a magnification
additionally to the scale bar? Or does the question make any sense at all?


Stephane



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From: microscopy.listserver-at-gmail.com
Date: Fri, 7 Sep 2018 16:46:40 -0500
Subject: [Microscopy] viaWWW:Job posting - Light Microscopy Specialist

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Email: plappleton-at-dundee.ac.uk Name: Paul Appleton

Organization: University of Dundee

Title-Subject: [Filtered] Job posting - Light Microscopy Specialist

Message: A great opportunity now available in the Dundee Imaging
Facility (Scotland, UK) - permanent position! Please get in touch with
me to find out more.



Light Microscopy Specialist at the University of Dundee

Reference Number: SLSC0470

Job Title: Light Microscopy Specialist

School: Life Sciences

Unit: DIF

Grade: Grade 7 (£31,604 - £38,833)

Closing Date: 07 October 2018

The Dundee Imaging Facility provides a world-leading core microscopy
resource to Researchers, Staff and Students in the life, biomedical, and
physical sciences, and across the University. . The Facility houses } 20
advanced light microscopy systems, including LSCM, WF-decon, SDCM, TIRF,
MPLSM, FLIM and 3DSIM technologies. New acquisitions include a diSPIM
and AiryScan systems. The Facility also houses electron microscopy and
atomic probe microscopy, which are used for research applications in the
life and physical sciences.

We are recruiting for an exceptional individual to join us as a Light
Microscopy Specialist within the facility to provide high level
technical/specialist expertise and to translate knowledge of advances in
the field of advanced imaging/light microscopy into research success.

The role holder will provide user training and support for advanced
microscopy systems housed in the facility including FLIM, TIRF, MPLSM,
3DSIM and SR LSCM. They will also assist users to troubleshoot imaging
experiments and proactively offer advice and support to solve problems
quickly. The role holder will work across both the School of Life
Sciences (City Campus) and the School of Medicine (Ninewells Campus).

This is an excellent opportunity to join the Dundee Imaging Facility in
a key role supporting the research and training activities of the
University. The Facility is a critical resource for staff working in a
wide range of disciplines in the Schools of Life Sciences, Medicine and
Science & Engineering.

Who we’re looking for:

• Advanced knowledge of all aspects of Light Microscopy
• Significant experience in advanced imaging
• Problem solving ability and able to work autonomously and clearly
communicate complex, specialist information
• Independent thinking skills, utilising information obtained from
published literature, collaborative discussions, and other sources
• Strong interpersonal, communication and presentation skills
• Appreciation and understanding of data protection, confidentiality,
equality and diversity legislation
• Knowledge of all aspects of Health & Safety, Good Laboratory Practice
and Research Integrity

We are one of the UK’s leading universities – internationally recognised
for our expertise across a range of disciplines and research
breakthroughs in multiple areas, including science, medicine and
engineering, amongst many others. Conveniently located on the banks of
River Tay, our main city-centre campus is at the heart of Dundee - an
up-and-coming, friendly, compact and affordable city with a rich
heritage in design and technology. Just a short walk from the V&A Museum
of Design Dundee, we’re close to both the train and bus stations. We
also have campuses at Ninewells Hospital and in Kirkcaldy which are
easily accessible via local transport links.

For further information about this position please contact Paul Appleton
at p.l.appleton-at-dundee.ac.uk. To find out more about the Dundee Imaging
Facility please visit
www.lifesci.dundee.ac.uk/technologies/dundee-imaging-facility.

We anticipate that interviews for this appointment will take place in
late October.

The diversity of our staff and students helps to make the University of
Dundee one of the top universities in the UK. Family friendly policies,
staff support networks for BME and LGBT staff, membership of Athena SWAN
and Stonewall, as well a full range of disability services, create an
enjoyable and inclusive place to work.

Apply Here:

https://www.jobs.dundee.ac.uk/fe/tpl_uod01.asp?s=4A515F4E5A565B1A&jobid=102497,4912145689&key=137857282&c=35253486652358&pagestamp=sewgppersgmnjkftlw


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From: John.Mardinly-at-asu.edu
Date: Sat, 8 Sep 2018 11:26:10 -0500
Subject: [Microscopy] Re: magnification on a side-mounted camera

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Take a lesson from NION-The NION microscopes do not have ANY magnification readout-just a scale bar and pixel size embedded in the digital image.

John Mardinly

} On Sep 7, 2018, at 2:45 PM, microscopy.listserver-at-gmail.com wrote:
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}
} X-from: Stephane Nizet {nizets2-at-yahoo.com}
} Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
}
}
} Dear colleagues,
}
} I am quite sure that I read a discussion about this before but I cannot
} find it anymore so I'm asking again, sorry for that.
} We have a side-mounted camera (Megaview III) on a tecnai G20 microscope.
} The magnification given by the microscope corresponds to the image on
} the phosphor screen.
} But since the camera is not in the same plane and the beam is not
} parallel, the magnification on the camera is different.
}
} Of course I calibrate the camera so my measurements on pictures taken by
} the camera are (more or less) right, but how can I know exactly at which
} magnification the picture has been taken?
} And, even more importantly, does it matter?
} Since the digital pictures can be printed at (almost) any size, this
} would mean that I end up with different magnifications, am I right?
}
} If the microscope tells me that I used a 40 000x magnification, what
} should I write in my digital picture if I want to write a magnification
} additionally to the scale bar? Or does the question make any sense at all?
}
}
} Stephane
}
}
}
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} 14, 53 -- Subject: Fwd: magnification on a side-mounted camera
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5, 105 -- From: John Mardinly {John.Mardinly-at-asu.edu}
5, 105 -- To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} ,
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5, 105 -- Subject: Re: [Microscopy] magnification on a side-mounted camera
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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Sep 2018 13:43:02 -0500
Subject: [Microscopy] Fwd: magnification on a side-mounted camera

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-------- Forwarded Message --------

X-from: Ribardire Michel {m.ribardiere-at-jeol.fr}



The magnification dpends on where you want to know
You can easily calculate from pixel size on the ccd sensor
Another possibily is where you read the image. Off course it depends of
your monitor size.
Usually it is better to tell the final magnification and the
magnification on the sensor
Anyway, the microbar Will be the best for mag calibration

Best regards
Michel



Envoy de mon Galaxy A5 2017 Orange


-------- Message d'origine --------
De : microscopy.listserver-at-gmail.com
Date : 07/09/2018 23:45 (GMT+01:00)
: Ribardire Michel {m.ribardiere-at-jeol.fr}
Objet : ***SPAM-PROBABLE*** [Microscopy] Fwd: magnification on a
side-mounted camera




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-------- Forwarded Message --------

X-from: Stephane Nizet {nizets2-at-yahoo.com}
Reply-To: Stephane Nizet {nizets2-at-yahoo.com}


Dear colleagues,

I am quite sure that I read a discussion about this before but I cannot
find it anymore so I'm asking again, sorry for that.
We have a side-mounted camera (Megaview III) on a tecnai G20 microscope.
The magnification given by the microscope corresponds to the image on
the phosphor screen.
But since the camera is not in the same plane and the beam is not
parallel, the magnification on the camera is different.

Of course I calibrate the camera so my measurements on pictures taken by
the camera are (more or less) right, but how can I know exactly at which
magnification the picture has been taken?
And, even more importantly, does it matter?
Since the digital pictures can be printed at (almost) any size, this
would mean that I end up with different magnifications, am I right?

If the microscope tells me that I used a 40 000x magnification, what
should I write in my digital picture if I want to write a magnification
additionally to the scale bar? Or does the question make any sense at all?


Stephane



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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Sep 2018 13:44:18 -0500
Subject: [Microscopy] Fwd: magnification on a side-mounted camera

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-------- Forwarded Message --------

X-from: Amalia Pasolli {amaliapasolli-at-gmail.com}



I would print the picture, measure the scale bar with a ruler, convert
to micrometers and divide by the number of micrometers indicated by the
scale.

Best,

AMALIA

On Fri, Sep 7, 2018 at 5:50 PM, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:





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-------- Forwarded Message --------

X-from:         Stephane Nizet {nizets2-at-yahoo.com
{mailto:nizets2-at-yahoo.com} }
Reply-To:       Stephane Nizet {nizets2-at-yahoo.com
{mailto:nizets2-at-yahoo.com} }


Dear colleagues,

I am quite sure that I read a discussion about this before but I cannot
find it anymore so I'm asking again, sorry for that.
We have a side-mounted camera (Megaview III) on a tecnai G20
microscope.
The magnification given by the microscope corresponds to the image on
the phosphor screen.
But since the camera is not in the same plane and the beam is not
parallel, the magnification on the camera is different.

Of course I calibrate the camera so my measurements on pictures
taken by
the camera are (more or less) right, but how can I know exactly at
which
magnification the picture has been taken?
And, even more importantly, does it matter?
Since the digital pictures can be printed at (almost) any size, this
would mean that I end up with different magnifications, am I right?

If the microscope tells me that I used a 40 000x magnification, what
should I write in my digital picture if I want to write a magnification
additionally to the scale bar? Or does the question make any sense
at all?


Stephane



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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Sep 2018 13:45:15 -0500
Subject: [Microscopy] Fwd: magnification on a side-mounted camera

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------

X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Dear Stephane,


You shouldn't worry about that as long as you have the pixel size well
calibrated on your CCD.

To have the scale bar right is what matters, as the magnification will
depend on the size of the image printed on a paper or projected on a screen.

But if you are still concerned about that, I suggest you to have a look
at ISO 20301:2017


Regards,


On Fri, 7 Sep 2018, 18:52 , {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:





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-------- Forwarded Message --------

X-from:         Stephane Nizet {nizets2-at-yahoo.com
{mailto:nizets2-at-yahoo.com} }
Reply-To:       Stephane Nizet {nizets2-at-yahoo.com
{mailto:nizets2-at-yahoo.com} }


Dear colleagues,

I am quite sure that I read a discussion about this before but I cannot
find it anymore so I'm asking again, sorry for that.
We have a side-mounted camera (Megaview III) on a tecnai G20
microscope.
The magnification given by the microscope corresponds to the image on
the phosphor screen.
But since the camera is not in the same plane and the beam is not
parallel, the magnification on the camera is different.

Of course I calibrate the camera so my measurements on pictures
taken by
the camera are (more or less) right, but how can I know exactly at
which
magnification the picture has been taken?
And, even more importantly, does it matter?
Since the digital pictures can be printed at (almost) any size, this
would mean that I end up with different magnifications, am I right?

If the microscope tells me that I used a 40 000x magnification, what
should I write in my digital picture if I want to write a magnification
additionally to the scale bar? Or does the question make any sense
at all?


Stephane



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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Sep 2018 13:46:40 -0500
Subject: [Microscopy] Fwd: magnification on a side-mounted camera

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Christopher Winkler {microwink-at-gmail.com}


Hello Stephane,

In Digital Micrograph, you can look at the image tags and it will
display the actual magnification of an image captured using a camera
located above or below the film chamber. I'm sure your camera uses
different software, but the magnification may be embedded in the image
tags as well. You can check with the vendor. You can also calculate
the difference in magnification by using the markings on the green
screen with a calibration sample or diffraction pattern. There are
other ways to compute magnification, and I'm sure you will receive
replies that are more helpful in that regard.

Ultimately, magnification is somewhat meaningless. The total field of
view (FOV) in physical units, the scale bar, and/or the pixel size in
physical units are much more important. As you point out,
magnification is relative. The physical size of the objects in your
images should not be. Generally a scale bar combined with the FOV is
enough for most, but it depends on your customer's needs.

I hope this was somewhat helpful. Cheers,
Chris

On Fri, Sep 7, 2018 at 5:48 PM {microscopy.listserver-at-gmail.com} wrote:
}
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}
} X-from: Stephane Nizet {nizets2-at-yahoo.com}
} Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
}
}
} Dear colleagues,
}
} I am quite sure that I read a discussion about this before but I cannot
} find it anymore so I'm asking again, sorry for that.
} We have a side-mounted camera (Megaview III) on a tecnai G20 microscope.
} The magnification given by the microscope corresponds to the image on
} the phosphor screen.
} But since the camera is not in the same plane and the beam is not
} parallel, the magnification on the camera is different.
}
} Of course I calibrate the camera so my measurements on pictures taken by
} the camera are (more or less) right, but how can I know exactly at which
} magnification the picture has been taken?
} And, even more importantly, does it matter?
} Since the digital pictures can be printed at (almost) any size, this
} would mean that I end up with different magnifications, am I right?
}
} If the microscope tells me that I used a 40 000x magnification, what
} should I write in my digital picture if I want to write a magnification
} additionally to the scale bar? Or does the question make any sense at all?
}
}
} Stephane
}
}
}
} ==============================Original Headers==============================
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--
Senior Research Associate
Nanoscale Characterization and Fabrication Laboratory
Institute for Critical Technology and Applied Science
Virginia Tech
(267) 496-0587

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From: jrminter-at-gmail.com
Date: Sat, 8 Sep 2018 17:03:52 -0500
Subject: [Microscopy] magnification on a side-mounted camera

Contents Retrieved from Microscopy Listserver Archives
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I agree with John Mardinly that a scale bar (preferably as an overlay
in the image) and pixel size embedded (I'm assuming in the metadata)
in the digital image are a minimum requirement for image recording.

I do like the idea of including a "nominal magnification" in the
metadata as well. That is really only the instrument setting. I always
found it useful to be able to record additional images under the same
conditions. I always liked the way Gatan recorded all of the settings
the microscope could measure (and a few input by the user at
acquisition time) in the metadata in images from DigitalMicrographs.
As many in the #rstats community are fond of noting, "your closest
collaborator is you six months from now and you don't respond to
email." Metadata saved my bacon many times...

Best regards,
John Minter

On Sat, Sep 8, 2018 at 12:26 PM {John.Mardinly-at-asu.edu} wrote:
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} Take a lesson from NION-The NION microscopes do not have ANY magnification readout-just a scale bar and pixel size embedded in the digital image.
}
} John Mardinly
}
} } On Sep 7, 2018, at 2:45 PM, microscopy.listserver-at-gmail.com wrote:
} }
} }
} }
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} }
} } -------- Forwarded Message --------
} }
} } X-from: Stephane Nizet {nizets2-at-yahoo.com}
} } Reply-To: Stephane Nizet {nizets2-at-yahoo.com}
} }
} }
} } Dear colleagues,
} }
} } I am quite sure that I read a discussion about this before but I cannot
} } find it anymore so I'm asking again, sorry for that.
} } We have a side-mounted camera (Megaview III) on a tecnai G20 microscope.
} } The magnification given by the microscope corresponds to the image on
} } the phosphor screen.
} } But since the camera is not in the same plane and the beam is not
} } parallel, the magnification on the camera is different.
} }
} } Of course I calibrate the camera so my measurements on pictures taken by
} } the camera are (more or less) right, but how can I know exactly at which
} } magnification the picture has been taken?
} } And, even more importantly, does it matter?
} } Since the digital pictures can be printed at (almost) any size, this
} } would mean that I end up with different magnifications, am I right?
} }
} } If the microscope tells me that I used a 40 000x magnification, what
} } should I write in my digital picture if I want to write a magnification
} } additionally to the scale bar? Or does the question make any sense at all?
} }
} }
} } Stephane
} }
} }
} }
} } ==============================Original Headers==============================
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} 5, 105 -- From: John Mardinly {John.Mardinly-at-asu.edu}
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} 5, 105 -- {Microscopy-at-Microscopy.com}
} 5, 105 -- Subject: Re: [Microscopy] magnification on a side-mounted camera
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5, 44 -- From jrminter-at-gmail.com Sat Sep 8 17:03:52 2018
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5, 44 -- From: John Minter {jrminter-at-gmail.com}
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5, 44 -- Subject: Re: [Microscopy] Re: magnification on a side-mounted camera
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From: zaluzec-at-microscopy.com
Date: Tue, 11 Sep 2018 16:40:58 -0500
Subject: [Microscopy] viaWWW:Employment Opportunity

Contents Retrieved from Microscopy Listserver Archives
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Email: bonnie_salyer-at-tedpella.com Name: Bonnie Salyer

Organization: Ted Pella, Inc.

Title-Subject: [Filtered] Opportunity

Message: Ted Pella, Inc. is seeking a Materials Science Product
Specialist to join our dynamic team in support of our sample preparation
product lines. This is a great opportunity to help develop and grow
product lines.
Ideal candidate will have a BS in Materials Science or related field,
with a minimum of 3 years SEM and 2 years TEM with hands-on specimen
prep for both, beyond an academic environment. Must have specimen prep
experience in semi-conductor industry. Must have LM experience. EDS or
WDS experience desired, AFM experience a plus.
Contact human_resources-at-tedpella.com for complete job posting or to
submit a resume. More information at www.tedpella.com



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From: sergei2-at-ornl.gov
Date: Wed, 12 Sep 2018 09:53:33 -0500
Subject: [Microscopy] November 1,2: Atom by atom fabrication via STEM electron beams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

The registration website for the workshop on atom by atom fabrication by
electron beam is now available at:

https://utconferences.eventsair.com/workshop-on-atom-by-atom-fabrication-via-electron-beams-and-scanning-probes/register

2. The invited speakers include:
(plenary) John Randall, Zyvex
(plenary) Toma Susi, U. Vienna
David Forrest, DOE EERE
Khershed Cooper, /NSF /
Ondrej Krivanek, Nion
Rick Silver, NIST
Stephen Jesse, ORNL
Adam Schwartzberg, Molecular Foundry
Dirk Englund, MIT
Marija Drndic, UPenn
Ezra Bussman, Sandia
Prineha Narang, Harvard
Joe Lyding, U. Illinois
Q. Ramasse, SuperSTEM
Travis Humble, ORNL
Aaron Stein, CFN BNL
R. Wolkow, U. Alberta

3. There are slots available for several invited, contributed, and
poster presentations!

Looking forward to hearing from you!

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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12, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
12, 37 -- Subject: November 1,2: Atom by atom fabrication via STEM electron beams
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From: microscopy.listserver-at-gmail.com
Date: Thu, 13 Sep 2018 17:06:08 -0500
Subject: [Microscopy] viaWWW: North Atlantic Microscopy Society (NAMS); Inaugural Symposium

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Email: glaevsky.lists-at-gmail.com Name: Gary Laevsky

Organization: North Atlantic Microscopy Society (NAMS)

Title-Subject: [Filtered] North Atlantic Microscopy Society (NAMS);
Inaugural Symposium November 1, 2018 at Princeton University

Message: Hello!

For registration, https://namsmicroscopy.com/meeting-registration

We are very proud and excited to announce the formation of a new
society, The North Atlantic Microscopy Society (NAMS). Geographically
centered at Princeton, NJ, we envision our coverage to span southern New
York, New Jersey and into Pennsylvania. NAMS was born not simply because
we noticed a distinct gap in regional cohesion. But because we are
passionate about microscopy in all its forms and believe that we are not
alone.
Edwin Hubble famously said, “Equipped with his five senses, man
explores the universe around him and calls the adventure Science.” We
believe that some of this exploratory instinct has been muted lately by
our disciplinary silos. Individually, we have become exceptional in our
specialties and do not take moments to appreciate the many discoveries
happening across the entire spectrum of science.
Our mission is to bridge these silos through the lens of microscopy.
We seek to achieve this mission by promoting microscopy education,
stimulating networking among microscopists, and disseminating microscopy
knowledge and skills to the public in the region.
Our first Symposium will be held at Princeton University on November
1, 2018. We are planning bi-annual symposia, with the fall event always
held at Princeton, and the spring event held at rotating locations
throughout the region (April '19 tentatively being held at Rockefeller
University). We are also planning smaller satellite meetings throughout
the academic year. Registration payment covers both symposium and
satellite meetings.

Please see below for schedule. We have a full day planned!

And, if you present a poster or a 5 minute lightning talk, the event is
FREE!!!

Abstracts accepted on registration page of website namsmicroscopy.com


8:15a Registration/Breakfast
9a-9:30a Opening Remarks
9:30a-10:30a EM talk
Esther Bullitt –
Boston University
10:30a-10:45a Break
10:45a-12p Break out into two rooms
6-5 min
Light talks
6-5 min EM
talks
12p-2:30p Lunch/vendor session/posters/PRISM
tour
2:30p-3:30p Keynote/Light talk
Hari Shroff - NIH
3:30p-3:45p Break
3:45p-5p Panel discussions
Cryo-EM sample prep
Mass Spec imaging
Lightsheet imaging
Big Data
5p- Organizational/Administrative
(pizza/beverages) (WE need help!!!)
Treasurer
Web Design
Secretary

NAMS Co-Founders
Gary Laevsky
Paul Shao
Tharan Srikumar


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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Sep 2018 08:54:24 -0500
Subject: [Microscopy] viaWWW: UGa Biological TEM Workshop

Contents Retrieved from Microscopy Listserver Archives
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop again!

Message: Our Biological TEM workshop is filling up but we have a few
seats left! This hands-on, three day workshop is W-F, Oct 24-26, 2018
covers conventional TEM chemical prep, briefly discusses older Cryo
techniques, negative staining, sectioning and other topics suggested by
you to help you get your work done, and/or learn a new skill.

We have a ton of fun while working hard for three days. For more
information, contact John at jphield-at-uga.edu or go to our website for
more information and registration
(https://gem.uga.edu/biological-tem-workshop/)

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From: zaluzec-at-microscopy.com
Date: Sun, 16 Sep 2018 18:46:48 -0500
Subject: [Microscopy] viaWWW:Workshop Announcement: Machine Learning approaches in High

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Email: whiteto-at-missouri.edu Name: Tommi A. White

Organization: University of Missouri Electron Microscopy Core

Title-Subject: [Filtered] Workshop Announcement: Machine Learning approaches in High Resolution
Microscopy Imaging at BIBM 2018

Message:

We will be hosting a day-long workshop on “Machine Learning approaches in High Resolution Microscopy
Imaging” (MLHRM 2018 - http://www.mlhrm.net/) at the 2018 IEEE International Conference on
Bioinformatics and Biomedicine December 3-6, 2018 in Madrid, Spain (http://orienta.ugr.es/bibm2018/).

This workshop aims to bring the researchers from computational and imaging fields together to focus
on the computational approaches for construction and analysis in high resolution imaging modalities.
Special emphasis will be on the applications of deep learning and other machine learning methods in
imaging modalities such as cryo-electron microscopy, FIB-SEM tomography, and fluorescence
super-resolution microscopy.

Topics include:
* Noise reduction in electron microscopy (EM) & super-resolution microscopy (SRM) modalities
* Signal detection and image reconstruction in SRM
* Image segmentation in EM modalities
* Image classification in EM modalities and SRM
* 3D reconstruction methods and pipelines in cryo-EM
* Characterization of single molecule structure using machine learning approaches
* Applications of high resolution microscopy in precision medicine
* Applications in single molecule tracking, multiphoton imaging


Original contributions in applications of deep learning and other machine learning methods in high
resolution microscopy including but not limited to noise reduction, detection, segmentation,
classification, and reconstruction of 2D and 3D models, as well as new approaches in 3D
reconstruction of single molecules are welcome. Contributions regarding other related modalities and
automated analysis methods such as single molecule tracking, multiphoton imaging, and combining
electron microscopy (EM) with super-resolution microscopy (STORM/PALM) will also be considered for
inclusion in the workshop program. Please submit a full-length original and unpublished research
contribution (up to 6 pages in IEEE 2-column format) through the online submission system.
Formatting instructions for paper submission are available here:
http://www.ieee.org/conferences_events/conferences/publishing/templates.html Paper submission
deadline has been extended to October 5th, 2018 and can be made here:
https://wi-lab.com/cyberchair/2018/bibm18/index.php

We look forward to seeing you in Madrid! Please contact us with any questions (whiteto-at-missouri.edu).

Dr. Tommi White, Electron Microscopy Core & Department of Biochemistry, University of Missouri, USA.
Dr. Filiz Bunyak, Department of Electrical Engineering and Computer Science, University of Missouri,
USA. Dr. Ilker Ersoy, Informatics Institute, Department of Pathology and Anatomical Sciences,
University of Missouri, USA.

Tommi A. White, Ph.D.
Assistant Professor of Biochemistry
Director, Electron Microscopy Core Facility
University of Missouri
W117 Veterinary Medicine Building
1600 East Rollins Street
Columbia, MO 65211
573-882-8304
WhiteTo-at-missouri.edu
http://emc.missouri.edu
on here

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From: jkrupp-at-deltacollege.edu
Date: Tue, 18 Sep 2018 18:35:11 -0500
Subject: [Microscopy] Full time instructor job at SJ Delta College

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Posting for a friend :)

San Joaquin Delta College in Stockton, California has a full time instructor position in Electron Microscopy available.

Here’s the link:


https://www.governmentjobs.com/careers/deltacollege/jobs/2203598/associate-professor-professor-of-electron-microscopy?keywords=electron&pagetype=jobOpportunitiesJobs


Don’t delay, I think it closes in a week.

This is my old job. Glad to answer any questions, but follow the link for all official business.

Retirement is delightful.

Happy Trails

Jon

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From: zaluzec-at-microscopy.com
Date: Wed, 19 Sep 2018 08:16:16 -0500
Subject: [Microscopy] viaWWW: CLEM Seminar at Max Planck Florida Institute

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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] CLEM Seminar at Max Planck Florida Institute

Message: The Electron Microscopy Facility of the Max Planck Florida Institute for Neuroscience
(Jupiter, FL) will host a new “labs-at-location” partnership launch event on October 11th, 2018. The
event includes a seminar focusing on the latest topics of Correlative Light-Electron Microscopy
(CLEM), a demo for the new ZEISS Focal Charge Compensation module on the 3View-Gemini SEM300 system
in the EM facility, and a partnership signing ceremony followed by a reception.

The event is open to all levels of researchers who are interested in utilizing the latest advances
in electron microscopy.
For more details and registration, please check our website;
http://mpfi.org/mpfi-zeiss-partnership

Registration is open until October 4th.


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From: stefan.diller-at-t-online.de
Date: Fri, 21 Sep 2018 07:55:41 -0500
Subject: [Microscopy] Automated scanning and stitching with Quanta 200 FEG-SEM

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Dear All,
I am looking for an automated solution to scan large image arrays with a Quanta 200 FEG-SEM.
Any ideas?
It should be not very costly since it is for student work...

Thanks,
Stefan



-----------------------------------------------------
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Arndtstrasse 22
D - 97072 Wuerzburg Germany
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From: vray-at-partbeamsystech.com
Date: Fri, 21 Sep 2018 10:53:07 -0500
Subject: [Microscopy] Re: Automated scanning and stitching with Quanta 200

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephan,

If your Quanta already has SW option for being controlled remotely, then
then MicroManager plugin for ImageJ could be something to look at:

https://micro-manager.org/

Another option would be trying to use either AutoIt, or WinAutomation,
or similar windows-scripting tool to simulate operator clicking on
buttons of Xp GUI for doing repetitive move-image-save operations.

All other solutions I can think of wouldn't fall into "not very costly"
category, as they would require either purchasing software from the OEM
(if available) or installing third-party scanning stage, etc...

Happy Scanning!
Valery

Valery Ray
www.linkedin.com/in/valeryray/
Also with REFINE Center, UCONN
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 (leave a message)
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Web: www.freudlabs.com

On 9/21/2018 8:55 AM, stefan.diller-at-t-online.de wrote:
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} Dear All,
} I am looking for an automated solution to scan large image arrays with a Quanta 200 FEG-SEM.
} Any ideas?
} It should be not very costly since it is for student work...
}
} Thanks,
} Stefan
}
}
}
} -----------------------------------------------------
} Stefan Diller - Scientific Photography
} Arndtstrasse 22
} D - 97072 Wuerzburg Germany
} ++49-931-7848700 Phone
} ++49-931-7848701 Fax
} ++49-175-7177051 Mobile
}
} Websites:
} www.nanoflight.info. NEW!
} www.stefan-diller.com
} www.electronmicroscopy.info
} www.elektronenmikroskopie.info
} www.assisi.de
} www.zwillingsprojekt.de
} -----------------------------------------------------
}
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 21 Sep 2018 16:44:21 -0500
Subject: [Microscopy] viaWWW:Colorado Microscopy Meeting

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Email: hlowers-at-usgs.gov Name: Heather Lowers

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Title-Subject: [Filtered] Colorado Microscopy Meeting

Message: The Mountain States Society of Electron Microscopists is hosting a dinner meeting Oct 3rd
from 5p-9p in Westminster Colorado.

PLEASE REGISTER BY SEPTEMBER 25!
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From: microscopy.listserver-at-gmail.com
Date: Fri, 21 Sep 2018 16:45:17 -0500
Subject: [Microscopy] viaWWW:FEI XL-30 ESEM Poor vacuum

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Email: daniel.vanhart-at-uic.com Name: Daniel VanHart

Organization: Universal Instruments

Title-Subject: [Filtered] FEI XL-30 ESEM Poor vacuum

Message: We are having difficulty reaching the "vac OK" state on the SEM. Typically, we would reach
it in a few minutes but something changed dramatically and now it takes more than 15 minutes and
sometimes it never gets there.
Can anyone suggest what to check first?

Any suggestions for troubleshooting would be appreciated.

Thank you,

Daniel VanHart

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From: jrminter-at-gmail.com
Date: Sat, 22 Sep 2018 07:36:59 -0500
Subject: [Microscopy] Re: viaWWW:FEI XL-30 ESEM Poor vacuum

Contents Retrieved from Microscopy Listserver Archives
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Hi Daniel,

My lab (I'm now retired) had an FEI Sirion built on a similar column.
I saw this many times. The usual culprit was degradation of the vacuum
hoses around the connectors. They would develop cracks and need to be
replaced. I would check there first. You don't say whether your pumps
are mechanical or (dry) scroll pumps. Each of these need periodic
maintenance. Follow the manufacturer's instructions.

Hope this helps.

Best regards,
John Minter

} Email: daniel.vanhart-at-uic.com Name: Daniel VanHart
} Title-Subject: [Filtered] FEI XL-30 ESEM Poor vacuum
}
} Message: We are having difficulty reaching the "vac OK" state on the SEM. Typically, we would reach
} it in a few minutes but something changed dramatically and now it takes more than 15 minutes and
} sometimes it never gets there.
} Can anyone suggest what to check first?
}
} Any suggestions for troubleshooting would be appreciated.
}
} Thank you,
}
} Daniel VanHart

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5, 41 -- Subject: Re: [Microscopy] viaWWW:FEI XL-30 ESEM Poor vacuum
5, 41 -- To: Microscopy-at-microscopy.com, daniel.vanhart-at-uic.com
5, 41 -- Content-Type: text/plain; charset="UTF-8"
==============================End of - Headers==============================




From: oshel1pe-at-cmich.edu
Date: Tue, 25 Sep 2018 08:46:23 -0500
Subject: [Microscopy] FW: cryoEM Postdoc position at Northwestern University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Forwarding for a colleague. Do NOT reply to me!
Phil

Vinayak P. Dravid
Abraham Harris Chaired Professor
Department of Materials Science & Engineering
Founding Director, NUANCE Center
Founding Director; SHyNE Resource (NNCI)
Co-Director, Global McCormick
Fellow; APS, AAAS, ACerS, MSA & MRS
Email:v-dravid-at-northwestern.edu
Website: www.northwestern.edu/vpdgroup
Phone 847-491-3537
-at-profdravid
www.facebook.com/NUANCE.Center/
IMMEDIATE OPEN POSITION - Postdoctoral Research Associate
Cryo-Electron Microscopy of Biomolecular Structures and Dynamics
NORTHWESTERN UNIVERSITY
A postdoctoral research associate position is immediately available at Northwestern University in the area of “Cryo Electron Microscopy” of proteins, related biomolecular structures, complexes and their conformational dynamics under external stimuli.
The candidate will work in the group of Professor Vinayak Dravid in the department of materials science & engineering, towards the 3D and 4D characterization of structures and dynamics of fusion proteins, megamolecules and related complexes, primarily by cryo and conventional scanning transmission and transmission electron microscopy (S/TEM). Select experiments will be performed with fluidic-cell scanned probe microscopy (FC-SPM) to probe dynamic conformation changes and local nanomechanical perturbations. The candidate will work closely with the NUANCE Center (NSF Center for Facility Excellence in the greater Midwest), which houses a comprehensive sample preparation and atomic-nanoscale characterization capabilities for soft, hard and hybrid structures.
Dravid’s research is part of an extensive cross-disciplinary and collaborative project across Chemistry, Biomedical Engineering, and Cell & Molecular Biology experts at Northwestern and University of Chicago.
As a result, the candidate will have ample opportunity to learn diverse aspects of synthetic biology, simulation and imaging of unconventional molecules.
The preferred candidate will have a doctoral degree in science or engineering fields. The preferred candidate will also have a proven track-record and established hands-on experience in the areas of advanced electron microscopy of biomolecules and soft matter. Experience should include extensive expertise and hands-on experience in cryo-EM of biomolecules, in image processing, and in programming/software for the reconstruction of protein based structures.
The position is available immediately for two years with the possibility for extension. Compensation will be commensurate with experience and expertise, with opportunities for research faculty appointment towards training and preparation for tenure-track faculty position.
Complete applications will include: Introduction letter, Curriculum Vitae, a brief Research Statement, Contact information for 3 references (name, postal & email address, phone number).
Materials should be submitted as a single PDF to amy.morgan-at-northwestern.edu and vdravid-at-northwestern.edu

-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab



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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Sep 2018 08:49:43 -0500
Subject: [Microscopy] viaWWW:Applications Scientist on TEM Materials Science

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: sorin.lazar-at-gmail.com


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Email: sorin.lazar-at-gmail.com Name: Sorin Lazar

Organization: Thermo Fisher Scientific

Title-Subject: [Filtered] Applications Scientist on TEM Materials Science

Message: Dear Microscopists,

I would like to draw your attention to an opening in the Eindhoven Nanoport at Thermo Fisher Scientific.
If you know someone interested it will be very much appreciated if you could please forward the link
below.

http://jobs.thermofisher.com/ShowJob/Id/360853/Applications-Scientist-TEM-Materials-Science/

Thanks and regards,
Sorin


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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Sep 2018 09:05:57 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:FEI XL-30 ESEM Poor vacuum

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Ravi Thakkar {ravi.thakkar369-at-gmail.com}




I had a same problem in my Hitachi SEM, the airline connected to one of the pressure valve was
leaking. Just check the all tubing connected to vacuum pump and pressure gauge. There must be some
leak somewhere.

With Thanks and Regards.
-------------------------------------------------------------------------
Ravindra Thakkar
Associate Scientist,
Kansas State University,
Manhattan, Kansas (USA)
-------------------------------------------------------------------------


On Fri, Sep 21, 2018 at 3:50 PM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: daniel.vanhart-at-uic.com {mailto:daniel.vanhart-at-uic.com} Name: Daniel VanHart

Organization: Universal Instruments

Title-Subject: [Filtered] FEI XL-30 ESEM Poor vacuum

Message: We are having difficulty reaching the "vac OK" state on the SEM.  Typically, we would
reach
it in a few minutes but something changed dramatically and now it takes more than 15 minutes and
sometimes it never gets there.
Can anyone suggest what to check first?

Any suggestions for troubleshooting would be appreciated.

Thank you,

Daniel VanHart

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From: microscopy.listserver-at-gmail.com
Date: Wed, 26 Sep 2018 09:06:25 -0500
Subject: [Microscopy] Fwd: open SPIM question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Smith, III, Julian P.S {smithj-at-winthrop.edu}

Hi,
Newbie hoping to assemble an open SPIM

Initially for imaging clarity-cleared embryonic chick tectum and for teaching students in our
microscopy class light-sheet microscopy.

Do I need more than 20mw of laser power?  Wavelengths are 552 and 488.
Thanks in advance,
Julian

--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
349 Columbia Ave
Rock Hill, SCÊ 29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net


==============================Original Headers==============================
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From: sergei2-at-ornl.gov
Date: Wed, 26 Sep 2018 10:57:55 -0500
Subject: [Microscopy] STEM Image libraries -> machine learning

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

My colleagues and I would like to bring to your attention the following
development aimed at faster adoption of machine learning methods across
electron microscopy community, and enable ML/AI application in
atomically resolved imaging. Modern machine learning is impossible
without large volumes of labeled data. To enable faster adoption of
machine learning methods in STEM, ORNL is working with Citrine
Informatics to share an open library of images for the specific case of
Si - vacancy complexes in graphene monolayers with plans to increase the
amount of data in the library over time. The initial library is
available at (https://doi.org/10.25920/0xv3-8459)

A paper that discusses the collection, analysis, and dissemination of
this data is available at https://arxiv.org/abs/1809.04256 The notebooks
for the analysis workflow will be available at PyCroscopy (on GitHub)
shortly, and can be requested directly from Maxim Ziatdinov
(ziatdinovma-at-ornl.gov {mailto:ziatdinovmax-at-gmail.com} )

We hope that this initiative becomes adopted by the community.
Please contact Malcolm Davidson (mdavidson-at-citrine.io
{mailto:mdavidson-at-citrine.io} ), the leader of Citrine's Open Data
Initiative, for any questions about Citrine's open data repository or ML
platform. We're putting the finishing touches on some tooling and will
share a step-by-step guide to our workflow in the coming months if
you're interested in sharing your STEM data openly on Citrine's platform.

Looking forward to the new opportunities and collaborations

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, Foresight Institute, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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11, 37 -- From: "Sergei V. Kalinin" {sergei2-at-ornl.gov}
11, 37 -- Subject: STEM Image libraries -} machine learning
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From: drix00-at-gmail.com
Date: Wed, 26 Sep 2018 19:46:03 -0500
Subject: [Microscopy] Employment Opportunity: Microscopist for Hydro-Quebec in Canada

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Hydro-Quebec Center of Excellence, a leader in battery material
research, is looking for a microscopist SEM and/or TEM at the
technician expert level to work in the characterization group which
includes 5 state-of-the-art SEM and TEM and many more instruments. The
job is well paid with very good social advantage and work conditions.
See the job posting at :

https://emploi.hydroquebec.com/job/Varennes-Technicien%28ne%29-expert-M%C3%A9tallurgie-Recherche-et-d%C3%A9veloppement-QC/359453517/

The Hydro-Quebec work environment is mostly in French, but the Center
has a lot of international employees and the work environment is in
French and English.

More information about the Center of Excellence:

https://www.hydroquebec.com/ce-electrification-transports-stockage-energie/

Contact me if you have any question.

Regards,
Hendrix

==============================Original Headers==============================
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From: mjgardes-at-i3detroit.org
Date: Thu, 27 Sep 2018 19:08:11 -0500
Subject: [Microscopy] SEM - Looking for AMRAY documentation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,
I heard that there has been some effort to archive AMRAY documentation and
schematics. I'm curious how that's going as I've got an AMRAY 1845 FE
that's new to me and any information I can find would be helpful for
understanding their way of doing things.

Thanks,
Matt Gardeski

==============================Original Headers==============================
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2, 38 -- From: Matthew Gardeski {mjgardes-at-i3detroit.org}
2, 38 -- Date: Thu, 27 Sep 2018 20:08:43 -0400
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From: microscopy.listserver-at-gmail.com
Date: Fri, 28 Sep 2018 08:21:02 -0500
Subject: [Microscopy] viaWWW:Looking for EM Service help

Contents Retrieved from Microscopy Listserver Archives
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Email: mike.toalson-at-elementpi.com Name: Mike Toalson

Organization: Element Pi, LLC

Title-Subject: [Filtered] Looking for EM Service help

Message: Due to our fast growth this year, we are in need of a SEM service engineer in the Northeast
US or Great Lakes area.

Can anyone recommend a Third Party EM Service company in these areas?

We would also entertain being contacted by anyone looking for work as a service engineer, installer
and trainer for both floor model and tabletop SEM.

Please contact
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From: microscopy.listserver-at-gmail.com
Date: Fri, 28 Sep 2018 21:54:08 -0500
Subject: [Microscopy] viaWWW:SEM Independent Sale Agent opportunity

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Email: mike.toalson-at-elementpi.com Name: Mike Toalson

Organization: Element Pi, LLC

Title-Subject: [Filtered] SEM Independent Sale Agent opportunity

Message: We are searching for an Independent Sales Agent interested in working with us for the 8
state region surrounding the Great Lakes area ideally based in the Chicago area but other locations
within the region are possible. We have both entry level floor model and compact SEM systems as well
as sample preparation tools. You will start with an active pipeline and established customers
already using our systems in the region.

Background in EM preferred but Optical Microscopy, AFM, SEM accessories and such is perfectly adequate.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 1 Oct 2018 08:41:40 -0500
Subject: [Microscopy] viaWWW:North Atlantic Microscopy Society Inaugural Symposium at

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Email: glaevsky-at-princeton.edu Name: Gary Laevsky

Organization: Princeton University

Title-Subject: [Filtered] Deadline 10/20/2018: North Atlantic Microscopy Society Inaugural Symposium
at Princeton University, November 1, 2018

Message: Hi All,



For registration, namsmicroscopy.com/meeting-registration


We are very proud and excited to announce the formation of a new society,
The North Atlantic Microscopy Society (NAMS). Geographically centered at
Princeton, NJ, we envision our coverage to span southern New York, New
Jersey and into Pennsylvania. NAMS was born not simply because we noticed a
distinct gap in regional cohesion. But because we are passionate about
microscopy in all its forms and believe that we are not alone.



Edwin Hubble famously said, Equipped with his five senses, man explores
the universe around him and calls the adventure Science. We believe that
some of this exploratory instinct has been muted lately by our disciplinary
silos. Individually, we have become exceptional in our specialties and do
not take moments to appreciate the many discoveries happening across the
entire spectrum of science.



Our mission is to bridge these silos through the lens of microscopy. We
seek to achieve this mission by promoting microscopy education, stimulating
networking among microscopists, and disseminating microscopy knowledge and
skills to the public in the region.



Our first Symposium will be held at Princeton University on November 1,
2018. We are planning bi-annual symposia, with the fall event always held
at Princeton, and the spring event held at rotating locations throughout
the region (April '19 tentatively being held at Rockefeller University). We
are also planning smaller satellite meetings throughout the academic year.
Registration payment covers both symposium and satellite meetings.



Please see below for schedule. We have a full day planned!



And, if you present a poster or a 5 minute lightning talk, the event is
FREE!!!



Abstracts accepted on registration page of website namsmicroscopy.com





8:15a Registration/Breakfast

9a-9:30a Opening Remarks

9:30a-10:30a EM talk

Esther Bullitt =E2=80=93=
Boston
University

10:30a-10:45a Break

10:45a-12p Break out into two rooms

6-5 min Light
talks

6-5 min EM talk=
s

12p-2:30p Lunch/vendor session/posters/PRISM
tour

2:30p-3:30p Keynote/Light talk

Hari Shroff - NIH

3:30p-3:45p Break

3:45p-5p Panel discussions

Cryo-EM sample prep

Mass Spec imaging

Lightsheet imaging

Big Data

5p-
Organizational/Administrative (pizza/beverages)
(WE need help!!!)

Treasurer

Web Design

Secretary





NAMS souvenir tote bags to the first 50 registered attendees!



Speakers (lightning talks) and poster presenters attend free! Looks great
on the CV! Sign up now and share your Science!



Spring symposium confirmed at Rockefeller April 5, 2019! Fall 2019 at
Princeton. Spring 2010 tentatively held at UPENN!



Almost 20 vendors! 10 vendor talks!


https://namsmicroscopy.com/meeting-registration





NAMS Co-Founders

Gary Laevsky

Paul Shao

Tharan Srikumar

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From: microscopy.listserver-at-gmail.com
Date: Wed, 3 Oct 2018 03:57:51 -0500
Subject: [Microscopy] Fwd: [Filtered] MicroscopyListserverviaWWW: Cryo vs std TEM use

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Email: mdelann1-at-jhmi.edu Name: michael delannoy

Organization: Hopkins SOM Microscope Facility

Title-Subject: [Filtered] Cryo vs std TEM use

Message: Hello,
I would like to know the feasibility of having a TEM operate on a weekly
basis both as a standard electron microscope and a cryo-electron
microscope. Is this practical?

thanks,
M Delannoy

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From: rcsencsits-at-belcan.com
Date: Wed, 3 Oct 2018 09:19:27 -0500
Subject: [Microscopy] RE: Fwd: [Filtered] MicroscopyListserverviaWWW: Cryo

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Dear M Delannoy

In general it is very possible to use a TEM in cryo or std mode intermixed, if the "cryo" use involves a "cryo holder" and not a fully chilled cryo-stage.
A JEOL 3200 cryo, would be a full cryo stage and not switch easily between projects.
Using a cryo-holder such as the one Gatan sells, with a LN2 reservoir and cryo shields so that the sample is protected during transfer to the column, it is quite fine. UC Berkeley has been doing cryo-TEM and non-cryo in the same CM series TEM for decades.

Most materials people do not fully understand polymer of biological "cryo" work, so there will be some learning involved. I've been lucky to have participated on the both sides of the TEM world.

Regards,
Roseann

Roseann Csencsits, PhD.
Research Scientist
Belcan Vallecitos Laboratories
Desk: 925.862.4345
rcsencsits-at-belcan.com



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Email: mdelann1-at-jhmi.edu Name: michael delannoy

Organization: Hopkins SOM Microscope Facility

Title-Subject: [Filtered] Cryo vs std TEM use

Message: Hello,
I would like to know the feasibility of having a TEM operate on a weekly basis both as a standard electron microscope and a cryo-electron microscope. Is this practical?

thanks,
M Delannoy

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16, 115 -- Subject: RE: [Microscopy] Fwd: [Filtered] MicroscopyListserverviaWWW: Cryo
16, 115 -- vs std TEM use
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16, 115 -- vs std TEM use
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From: Reinhard.Rachel-at-biologie.uni-regensburg.de
Date: Wed, 3 Oct 2018 11:33:47 -0500
Subject: [Microscopy] Cryo vs std TEM use

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} } } 03.10.2018 at 10:58:
} I would like to know the feasibility of having a TEM operate on a weekly
} basis both as a standard electron microscope and a cryo-electron
} microscope. Is this practical?

Dear Michael,
There is no general answer, honestly.
It depends on the facility, it very much depends on the training of the various users you have / you have to get trained and to supervise, on the equipment, and it depends on the TEM that you have.
Yes, we do it, not on weekly basis, but with daily varying schedules, and this works. Fine.
Little problems, only, which are due to the limited training of the users, rather than the TEM.
If you know exactly what your TEM is able to do and to tolerate, and what your users know, then YES, it is possible.
Then, I do not see any reason why a weekly schedule would not work.
kind regards,
Reinhard


--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29
Elected member of the IFSM board

Next microscopy conferences:
- Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin
- EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference)
- MC2021 in Vienna (D-A-CH conference)
- next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz




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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Oct 2018 01:01:38 -0500
Subject: [Microscopy] viaWWW:New video tutorials for DTSA-II

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Email: nicholas.ritchie-at-nist.gov Name: Nicholas Ritchie

Organization: NIST

Title-Subject: [Filtered] New video tutorials for DTSA-II

Message: Over the years, many people have asked about video tutorials
for NIST DTSA-II. NIST DTSA-II provides software for quantification and
simulation of electron-generated energy dispersive x-ray spectra. It is
used all over the world in both the laboratory and class-room environments.

Recently, in response to the requests, we've created half-a-dozen videos
and published them on YouTube. The YouTube channel is here:
https://www.youtube.com/channel/UCt4nKyhfFQ8xecHyuTnCvIA

(Or search for DTSA-II on YouTube)

Currently, the videos cover an introduction to DTSA-II, quantification,
simulation and a couple of other topics. Subscribe to the channel and
you will be notified when new videos are published.

Hopefully, you find the videos helpful.

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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Oct 2018 01:02:40 -0500
Subject: [Microscopy] viaWWW:Sample Prep Advice for Thin Film

Contents Retrieved from Microscopy Listserver Archives
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Email: mike.toalson-at-elementpi.com Name: Mike Toalson

Organization: element Pi, LLC

Title-Subject: [Filtered] Sample Prep Advice for Thin Film

Message: Hoping to get some advice on sample prep for using SEM to
measure CVD film thickness via cross section.

Film is sputtered Aluminum with nominal 1 micron thickness on a
fluoropolymer substrate using approx 20-40kX magnification.

The aluminum film is too brittle for a razor cut and flakes off or
doesn't present a clean edge for measurement. We have tried with some
success encapsulating with epoxy support layer before cutting. Also
using LN2 for freeze fracture.

Any suggestions how to prepare and mount sample would be much appreciated!!

Mike Toalson
element Pi

Login Host: 47.208.173.229
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From: microscopy.listserver-at-gmail.com
Date: Fri, 5 Oct 2018 12:06:09 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Sample Prep Advice for Thin Film

Contents Retrieved from Microscopy Listserver Archives
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X-from: Lou Ann Miller {turtlelam-at-comcast.net}

Typically for SEM, it is not the section we go for, but the block
itself. The ultramicrotome would be used on the embedded block to
“polish the surface”. This means taking as small as 60nm slices, gently
on a small surface, say 1/2 mm, using a diamond knife and sectioning out
onto a water boat on the knife

Possible if the aluminum is very very thin. If it will separate from the
block at all with a razor, then using an old used diamond is worth a try
first. Usually the trim by hand step with the feel of it under my
fingers is how I can tell. The flaking may be thickness dependent, does
it “eat” the razor blade? If it eats the blade, it will harm the diamond.

If that does not work, someone more experienced with grinding techniques
may have some advice.

This can be put into a holder that grabs the block, or some may epoxy to
a stub. If any gluing is done, it needs to pump down in vacuum for 2-3
days before putting it to any SEM

Lou Ann Miller
MRL University of Illinois

Sent from my iPad

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} Organization: element Pi, LLC
}
} Title-Subject: [Filtered] Sample Prep Advice for Thin Film
}
} Message: Hoping to get some advice on sample prep for using SEM to
} measure CVD film thickness via cross section.
}
} Film is sputtered Aluminum with nominal 1 micron thickness on a
} fluoropolymer substrate using approx 20-40kX magnification.
}
} The aluminum film is too brittle for a razor cut and flakes off or
} doesn't present a clean edge for measurement. We have tried with some
} success encapsulating with epoxy support layer before cutting. Also
} using LN2 for freeze fracture.
}
} Any suggestions how to prepare and mount sample would be much appreciated!!
}
} Mike Toalson
} element Pi
}
} Login Host: 47.208.173.229
} Listserver Email Form V - 20120416
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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Oct 2018 09:21:11 -0500
Subject: [Microscopy] viaWWW:Career Opportunity: Transmission Electron Microscopy (TEM)

Contents Retrieved from Microscopy Listserver Archives
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X-from: Liang, Fengxia {Fengxia.Liang-at-nyulangone.org}



Hi Delannoy,

We don’t have any problem to switch between standard TEM and cryoEM with
our Talos120C TEM.

Best,
Alice
*----------------------------*
*Alice F. Liang, Ph.D*

Director of Microscopy Laboratory; Associate Professor of Cell Biology

NYU Langone Health, NYU School of Medicine

540 First Avenue, SK2 EM Suite, New York, NY  10016

T 212-263-7644;F 212-263-7643; Fengxia.liang-at-nyumc.org

https://med.nyu.edu/research/research-resources/scientific-cores-shared-resources/microscopy-laboratory


X-from: "microscopy.listserver-at-gmail.com
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{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} }
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X-from: robert.morris-at-unnpp.gov


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Email: robert.morris-at-unnpp.gov Name: Robert Morris

Organization: Naval Nuclear Laboratory (NNL)

Title-Subject: [Filtered] Career Opportunity: Transmission Electron
Microscopy (TEM) Expert

Message: Naval Nuclear Laboratory(NNL) - New York, currently has an
opening for a highly skilled Transmission Electron Microscope (TEM)
Scientist. At the NNL, we develop advanced naval nuclear propulsion
technology, ensuring the safety and reliability of our Navy's submarine
and aircraft carrier fleets. This individual will work in an advanced
materials characterization laboratory and be responsible for operating
and maintaining a FEI Tecnai TEM (new TEM to be procured in 2020).
Analysis work: failure analysis investigations, characterization of
manufacturing induced features, analyses of material microstructures and
chemistries to understand the fundamentals of corrosion and cracking
mechanisms.

Microscopy work: high resolution imaging, energy dispersive
spectroscopy, electron energy loss spectroscopy, and crystallographic
analyses.

Collaboration work: team with other colleagues to conducting sample
preparation using Focused Ion Beam (FIB), electropolishing, laser
ablation, and ion milling techniques.
For more information, please find the posting at:
https://navalnuclearlab.energy.gov/jobsearch/job-details/transmission-electron-microscopy-tem-expert/21840/1/

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From: microscopy.listserver-at-gmail.com
Date: Mon, 8 Oct 2018 09:23:58 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Sample Prep Advice for Thin Film

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Christopher Winkler {microwink-at-gmail.com}


Hello Mike,

Two different ion beam polishing techniques should work for a sample
like this, assuming you have access to such equipment. The first and
easiest thing to do would be to cut a cross section view using a dual
beam (FIB). We've done samples like this before and they turn out
well, especially if you're only interested in the Al layer thickness.
Another way to prepare a decent cross section is to use a broad beam
ion polisher, though you may need to use a cryostage to keep the
fluoropolymer substrate from charring and causing the Al layer to
delaminate. Both the FIB and broad beam polisher would avoid any
possibility of smearing and delamination caused by microtomy and
conventional polishing. If you don't have access to such equipment
then I'm sure you can locate a lab nearby that does.

Good luck,
Chris

On Fri, Oct 5, 2018 at 2:07 AM {microscopy.listserver-at-gmail.com} wrote:
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} Email: mike.toalson-at-elementpi.com Name: Mike Toalson
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} Organization: element Pi, LLC
}
} Title-Subject: [Filtered] Sample Prep Advice for Thin Film
}
} Message: Hoping to get some advice on sample prep for using SEM to
} measure CVD film thickness via cross section.
}
} Film is sputtered Aluminum with nominal 1 micron thickness on a
} fluoropolymer substrate using approx 20-40kX magnification.
}
} The aluminum film is too brittle for a razor cut and flakes off or
} doesn't present a clean edge for measurement. We have tried with some
} success encapsulating with epoxy support layer before cutting. Also
} using LN2 for freeze fracture.
}
} Any suggestions how to prepare and mount sample would be much appreciated!!
}
} Mike Toalson
} element Pi
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From: jacob.kabel-at-ubc.ca
Date: Tue, 9 Oct 2018 11:30:30 -0500
Subject: [Microscopy] Job Posting - UBC Vancouver

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,

The department of Materials Engineering at UBC's Point Grey campus in
Vancouver, BC has an opening for someone to run their electron
microscopy lab.  The posting can be found here:
https://www.hr.ubc.ca/careers-postings/staff.php as job posting 31497.

The short summary is as follows:

Engineering Technician IV
This position supports research and teaching activities in Materials
Engineering. The position is primarily responsible for the operation and
maintenance of the Department's Microscopy Laboratory including the
polishing lab and one SEM in the AMPEL facility. The Microscopy
Laboratory equipment includes optical and scanning electron microscopes,
polishing equipment and X-Ray diffraction equipment. Technician is
responsible for instructing and training users (both academic and
commercial clients) in safe operation of the equipment.
This position supports research and teaching labs when needed. Works
with students and research staff to assist in developing experimental
and testing procedures. Conducts minor maintenance and repairs. Member
of the department Safety committee.

--

Jacob Kabel
Director, Electron-Microbeam/X-Ray Diffraction Facility
Department of Earth, Ocean & Atmospheric Sciences
6339 Stores Road
The University of British Columbia
Vancouver, BC, Canada
V6T 1Z4


==============================Original Headers==============================
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7, 34 -- To: {Microscopy-at-microscopy.com}
7, 34 -- From: Jacob Kabel {jacob.kabel-at-ubc.ca}
7, 34 -- Subject: Job Posting - UBC Vancouver
7, 34 -- Message-ID: {ca24500c-d9f8-cbf5-5bf7-0f73a1cb88fd-at-ubc.ca}
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From: bradford.ross-at-botany.ubc.ca
Date: Tue, 9 Oct 2018 15:56:29 -0500
Subject: [Microscopy] Materials EM Tech Position at UBC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

Just throwing up this job opening left by my colleague who moved to a different lab/department. I am not associated with or responsible for this listing, so please do not contact me to inquire about it.

https://www.hr.ubc.ca/careers-postings/staff.php Job ID: 31497

Job Summary

This position supports research and teaching activities in Materials Engineering. The position is primarily responsible for the operation and maintenance of the Department's Microscopy Laboratory including the polishing lab and one SEM in the AMPEL facility. The Microscopy Laboratory equipment includes optical and scanning electron microscopes, polishing equipment and X-Ray diffraction equipment. Technician is responsible for instructing and training users (both academic and commercial clients) in safe operation of the equipment.

This position supports research and teaching labs when needed. Works with students and research staff to assist in developing experimental and testing procedures. Conducts minor maintenance and repairs. Member of the department Safety committee.

Cheers,
Bradford Ross

Electron Microscopy Technician
BioImaging Facility
University of British Columbia
Cunningham Building Rm. 64
2146 East Mall
Vancouver, B.C.
V6T 1Z4

phone 604-822-6996









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From: microscopy.listserver-at-gmail.com
Date: Fri, 12 Oct 2018 10:47:04 -0500
Subject: [Microscopy] viaWWW: Tensile sample holder for tecnai

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Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy

Organization: CNRS

Title-Subject: [Filtered] Tensile sample holder for tecnai

Message: Hi,
I am looking for a tensile sample holder for a Tecnai F20 equipped with
a S-Twin pole piece. So far I only found Gatan as manufacturer. Do you
know other manufacturers?
Thank you for your help
Best regards,

Eric LEROY Directeur de la plateforme microscopie lectronique ICMPE -
UMR 7182 2/8, rue Henri Dunant
94320 THIAIS T : 01.49.78.12.09
F : 01.49.78.12.03

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From: microscopy.listserver-at-gmail.com
Date: Sat, 13 Oct 2018 01:19:55 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Tensile sample holder for tecnai

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------
X-from: Chris Jones {C.Jones-at-nhm.ac.uk}

Have you tried Deben in the UK? They manufacture many stages,
attachments, etc for electron beam instruments.


Good luck!

Chris



------------------------------------------------------------------------
*From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
*Sent:* 12 October 2018 16:48
*To:* Chris Jones
*Subject:* [Microscopy] viaWWW: Tensile sample holder for tecnai



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---------------------------------------------------------------------------

Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy

Organization: CNRS

Title-Subject: [Filtered] Tensile sample holder for tecnai

Message: Hi,
I am looking for a tensile sample holder for a Tecnai F20 equipped with
a S-Twin pole piece. So far I only found Gatan as manufacturer. Do you
know other manufacturers?
Thank you for your help
Best regards,

Eric LEROY Directeur de la plateforme microscopie lectronique ICMPE -
UMR 7182 2/8, rue Henri Dunant
94320 THIAIS T : 01.49.78.12.09
F : 01.49.78.12.03

Login Host: 193.49.164.50
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



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From: microscopy.listserver-at-gmail.com
Date: Sun, 14 Oct 2018 21:37:38 -0500
Subject: [Microscopy] viaWWW: Charging in FIB

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Email: weutro-at-yahoo.com Name: GORDON

Title-Subject: [Filtered] Charging in FIB

Message: I need some advice on sample prep for FIB.
I have packaged parts, with gold bond wires, that goes into a FIB to do
circuit edits. Due to the samples being in a package, the samples would
charge. If I coat the sample, it would help the charging issue but then
I need to remove the coat after I'm done with the FIB edits. Does anyone
have any suggestions on what to coat it with and how to remove the coat
afterwards?
Thanks
Gordon

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From: microscopy.listserver-at-gmail.com
Date: Sun, 14 Oct 2018 21:38:35 -0500
Subject: [Microscopy] viaWWW: Northern California Society for Microscopy Fall Meeting Nov

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Email: rcsencsits-at-belcan.com

Name: Roseann Csencsits

Organization: Belcan Vallecitos Laboratories

Title-Subject: [Filtered] Northern California Society for Microscopy
Fall Meeting Nov 8th in Pleasanton, CA

Message: Join us for lunch, lectures and socializing, Thursday November 8.

RSVP via email to info-at-ncsmicroscopy.org by October 15.

11:30 - 11:45 am Check-in; demo sign up
11:45 - 12:00 pm Lunch, seating for lectures
12:00 - 2:00 pm Lectures
De Wood, USDA, “Agricultural Applications of Microscopy”

Justin Ondry, UC Berkeley,"Understanding the Removal Pathways of
Dislocations in Imperfectly Attached Nanocrystals using in-situ HRTEM”
Matt Hauwiller, UC Berkeley, “Utilizing Graphene Liquid Cell TEM to
Elucidate the Mechanisms ofNon-Equilibrium Etching of Metallic Nanocrystals”

Ray Twesten, Gatan, “Advanced EELS acquisition and analysis”


2:00-2:10 pm NCSM Bylaws Discussion

2:10-3:30 pm Coffee, snacks, conversation, tour/demos of Gatan’s EM labs



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From: microscopy.listserver-at-gmail.com
Date: Sun, 14 Oct 2018 21:39:20 -0500
Subject: [Microscopy] viaWWW:OCT embedded sample prep

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Email: lavoie-at-uw.edu Name: Ellen Lavoie

Organization: University of Washington

Title-Subject: [Filtered] OCT embedded sample prep

Message: Hello Everyone,

I have an interesting one here...I'd like to take a previously unfixed
(mouse) tissue sample that has been frozen in OCT and get it to the
point of being embedded in epoxy for TEM. Yes, I know it can be
sectioned with cryomicrotome then imaged in cryo but that's not an
option right now.
My thought involves HPF but not sure how to transition it without
dealing with a big mess.
Thoughts? If so please message me at lavoie-at-uw.edu

Cheers,
Ellen Lavoie UW MAF
Seattle, WA

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From: microscopy.listserver-at-gmail.com
Date: Sun, 14 Oct 2018 21:40:39 -0500
Subject: [Microscopy] =?UTF-8?Q?viaWWW:SCSMM_2018_fall_meeting_=c2=96_Nov_14=2c_2018?=

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Email: zhuoli-at-coh.org Name: Zhuo Li

Organization: City of Hope

Title-Subject: [Filtered] SCSMM 2018 fall meeting – Nov 14, 2018

Message: You are welcome to come to the Southern California Society for
Microscopy and Microanalysis (SCSMM) 2018 fall meeting on Wednesday,
November 14th (one week before Thanksgiving) at City of Hope Beckman
Center Argyros Auditorium. The invited speakers are Dr. Elena Mirada
from Cal State Northridge, and Dr. Jacob Berlin from City of Hope
Beckman Research Institute. In order to register, please sign up on-line
using the link
(https://imri.uci.edu/content/2018-fall-meeting-registration#overlay-context=content/2018-fall-meeting-registration)
no later than 5 p.m. Friday, November 9th. On-line registration is required.

Sincerely,
SCSMM board
www.scsmm.org
https://www.facebook.com/microscopymicroanalysis


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From: jon-at-cntech.co.uk
Date: Mon, 15 Oct 2018 03:32:38 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Tensile sample holder for tecnai

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

CN Tech based in Cambridgeshire UK, manufactures and distributes Swift Tensile stages in Europe. We also have representatives around the world, which include Electron Microscopy Sciences in the USA.

We specialise in customisable tensile stages to fit any microscope with or without heating and chilling.

http://www.swift-instruments.com
http://www.cntech.co.uk

I would be happy to discuss your requirements and the solutions we have for you.

Best Regards,

Jon Nottingham

CN Technical Services Limited
Office: +44 (0)1354 669899
Email: jon-at-cntech.co.uk
Web: www.cntech.co.uk


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Sent: 13 October 2018 13:05
To: Jon Nottingham {jon-at-cntech.co.uk}




-------- Forwarded Message --------
X-from: Chris Jones {C.Jones-at-nhm.ac.uk}

Have you tried Deben in the UK? They manufacture many stages, attachments, etc for electron beam instruments.


Good luck!

Chris



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*Sent:* 12 October 2018 16:48
*To:* Chris Jones
*Subject:* [Microscopy] viaWWW: Tensile sample holder for tecnai



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Email: eric.leroy-at-icmpe.cnrs.fr Name: Eric Leroy

Organization: CNRS

Title-Subject: [Filtered] Tensile sample holder for tecnai

Message: Hi,
I am looking for a tensile sample holder for a Tecnai F20 equipped with
a S-Twin pole piece. So far I only found Gatan as manufacturer. Do you
know other manufacturers?
Thank you for your help
Best regards,

Eric LEROY Directeur de la plateforme microscopie lectronique ICMPE -
UMR 7182 2/8, rue Henri Dunant
94320 THIAIS T : 01.49.78.12.09
F : 01.49.78.12.03

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From: sergei2-at-ornl.gov
Date: Mon, 15 Oct 2018 08:57:11 -0500
Subject: [Microscopy] 2 days remaining - Workshop on atomic fabrication by electron beams

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

There are two days (October 17 deadline) remaining for registration for
the workshop “Atom by atom fabrication via electron beams and scanning
probes”, to be held at CNMS, Oak Ridge National Laboratory on November
1,2. This workshop will bring together experts in scanning probe atomic
fabrication and emerging field of atomically resolved electron beam
matter manipulation, to highlight the recent advances and opportunities
and serve as a much-needed seed to establish its rapid growth. It will
provide a forum to present recent achievements in electron beam
manipulation, novel opportunities for instrumental development enabled
by the availability of high speed data analytic tools and machine
learning, integration of atomic-scale device engineering into
semiconductor workflows, and opportunities for quantum information systems.

{https://utconferences.eventsair.com/workshop-on-atom-by-atom-fabrication-via-electron-beams-and-scanning-probes/register} https://utconferences.eventsair.com/workshop-on-atom-by-atom-fabrication-via-electron-beams-and-scanning-probes/register

The confirmed plenary and invited speakers include Toma Susi (U. Vienna)
and John Randall (Zyvex), as well as David Forrest (DOE), and Khershed
Cooper (NSF). A number of participants from semiconductor and quantum
computing industry and DARPA are also participating, so the workshop can
offer great opportunities for networking. The partial program (allowing
for additional invited and regular contributions based on submissions)
is provided below.

Sergei

*Day 1:*

*8:30 – 9:15*J. Randall (Zyvex), { {V% - /Digital Atomic Scale
Fabrication: Moore's Law Inverted/

*9:15 – 10:00 *T. Susi (U. Vienna), /Electron-beam manipulation of
graphene impurities: competing processes and modeling challenges/

*10.00 – 10.30*David Forrest (DOE EERE), /Atomically Precise
Manufacturing for Energy Applications/

*10:30 - 11:00*/Break/

*11:00 - 11:30*A.L. Bleloch (Nion), /Precision control of the electron
beam and the sample environment in the STEM/

*11:30 – 12:00*Rick Silver (NIST), /Robust Fabrication and Measurement
of Atomically Precise Devices/

*12:00 – 12:15*K. Cooper (NSF), /NSF Nano and Advanced Manufacturing
Research at NSF/

*12:15 – 12:30*(slot)

*12:30 - 13:30*/Lunch/

*13:30 - 14:00*Stephen Jesse, /Scanning Transmission Electron Microscopy
for Atomic Manipulation: strategies for in-line image and spectral
analysis for feedback and controls/

*14:00 - 14:30*A. Schwartzberg (Molecular Foundry), TBD

*14:30 - 15:00*Dirk Englund (MIT), TBD

*15:00 - 15:30*/Break/

*15:30 - 16:00*M. Drndic (UPenn), TBD

*16:30 - 17:00*Ezra Bussmann (Sandia), TBD

*17:00 - 17:30*R. Moheimani (UT Dallas), /Non-raster Scan Methods for
High-speed Scanning Probe Microscopy/

Poster session

*Day 2:*

*8:30 – 9:00*P. Narang (Harvard), TBD

*9:00 – 9:30 *J. Lyding (UIUC) /Nanofabrication on Silicon/

*9:30 – 10:00 *D. Kepaptsoglou (SuperSTEM), /Single atom modification of
2D materials: fabrication and electronic structure/

*10:30 - 11:00*/Break/

*11:00 - 11:30*T. Humble (ORNL), /Atomistic Modeling and Simulation of
Quantum Devices/

*11:30 – 12:00*A. Stein (CFN BNL)

*12:00 – 12:30*R. Wolkov (U. Alberta)

*12:30 - 13:30*/Lunch/

*13:30 - 14:00*(slot)

*14:00 - 14:30*(slot)

*14:30 - 15:00*(slot)

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


==============================Original Headers==============================
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40, 37 -- Subject: 2 days remaining - Workshop on atomic fabrication by electron beams
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From: microscopy.listserver-at-gmail.com
Date: Mon, 15 Oct 2018 15:56:55 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Charging in FIB

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Valery Ray {webmaster-at-partbeamsystech.com}

Hi Gordon,

If you set proper GAE processes and use primary ion beam currents
appropriate for edits, then there wouldn't be any problems with surface
charging or ESD damage.

If there is no time/bandwidth/money/expertise to accomplish the above,
then there are two surface coating options suitable for brute-force
elimination of surface charging during FIB circuit edit: (a) carbon,
either evaporated or PIPS, or conductive polymers, either spin-coated or
ultrasonic-nozzle dispensed. Carbon deposited with thickness of about
20nm provides sufficient charge dissipation for CE-appropriate beam
currents while having little to no influence on regular operation of
most ICs, so it can be left on in most cases. If removal is required,
then O2 plasma cleaner ( {20W), or ozone asher, or UV cleaner would work
(from fastest to slowest) depending on sensitivity of the device.
Conductive polymers simply washed away after the edit site has been
capped an sealed.

Google for "Free of charge FIB circuit edit of ICs" and "New FIB tricks
with old conductive polymers"

Happy editing!
Valery

P.S. I am assuming that all pins of device under edit already have solid
connection to the stage of your CE FIB.

Valery Ray
Also with REFINE Center, UCONN
==============================
PBS&T, MEO Engineering Company
290 Broadway, Suite 298
Methuen, MA 01844, USA
Phone: +1-978-305-0479 (leave a message)
E-mail: vray-at-partbeamsystech.com
Web: www.partbeamsystech.com
Web: www.freudlabs.com

On 10/14/2018 10:38 PM, microscopy.listserver-at-gmail.com wrote:
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}
} X-from: weutro-at-yahoo.com
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form at
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} Email: weutro-at-yahoo.com Name: GORDON
}
} Title-Subject: [Filtered] Charging in FIB
}
} Message: I need some advice on sample prep for FIB.
} I have packaged parts, with gold bond wires, that goes into a FIB to do
} circuit edits. Due to the samples being in a package, the samples would
} charge. If I coat the sample, it would help the charging issue but then
} I need to remove the coat after I'm done with the FIB edits. Does anyone
} have any suggestions on what to coat it with and how to remove the coat
} afterwards?
} Thanks
} Gordon
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From: microscopy.listserver-at-gmail.com
Date: Mon, 15 Oct 2018 15:57:28 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Charging in FIB

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-------- Forwarded Message --------

X-from: Integrity Scientific Ltd {contact-at-integrityscientific.com}



Hi Gordon,
Use your micro manipulator to earth the local region. But be careful
not to blow any sensitive circuits.

Richard

} On 15 Oct 2018, at 03:38, microscopy.listserver-at-gmail.com wrote:
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} Email: weutro-at-yahoo.com Name: GORDON
}
} Title-Subject: [Filtered] Charging in FIB
}
} Message: I need some advice on sample prep for FIB.
} I have packaged parts, with gold bond wires, that goes into a FIB to do
} circuit edits. Due to the samples being in a package, the samples would
} charge. If I coat the sample, it would help the charging issue but then
} I need to remove the coat after I'm done with the FIB edits. Does anyone
} have any suggestions on what to coat it with and how to remove the coat
} afterwards?
} Thanks
} Gordon
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From: sergei2-at-ornl.gov
Date: Tue, 16 Oct 2018 13:10:02 -0500
Subject: [Microscopy] 2 Postdoctoral positions in STEM - ORNL

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}


Hi Gordon,

Sorry for the shameless self-promotion, but we actually published a
paper a little while back about using conductive polymers (used for
e-beam lithography) in the FIB. We were using it on flat wafer samples,
but you might be able to adapt the technique we described for your
needs. Most of the polymers are water soluble and can be removed with a
rinse afterwards. Please let me know if you need a PDF copy of the article.

https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/teaching-an-old-material-new-tricks-easy-and-inexpensive-focused-ion-beam-fib-sample-protection-using-conductive-polymers/7E9EAE6546D968E9537A56CFDB70D6AA

Good luck,
Josh

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Sent: Sunday, October 14, 2018 22:40
To: Taillon, Joshua A. (Fed) {joshua.taillon-at-nist.gov}

Dear colleagues

The STEM group at ORNL has open two postdoctoral research positions:

1. Postdoctoral Research Associate – Electron Microscopy of Quantum
Materials / NB50693574
2. Postdoctoral Research Associate - STEM of Heterostructured
Quantum Materials / NB50694303

The descriptions are as following:

Electron Microscopy of Quantum Materials:
We are seeking a Postdoctoral Research Associate to conduct research on
the atomistic origins of quantum phenomena in
novel material systems using high-resolution electron microscopy and
spectroscopy combined with machine learning and
artificial intelligence tools. Examples of relevant physical systems
include spin-liquid layered halides, charge-density wave
dichalcogenides, and other 2D materials. In this role, you will work
closely with the scanning transmission electron
microscopy (STEM) team at ORNL to conduct research using high-spatial
and energy resolution tools, and you will also
collaborate with machine learning experts at ORNL that will include deep
learning and simulation efforts on the Summit
supercomputer. This position resides in the Electron & Atom Probe
Microscopy Group in the Center for Nanophase Materials
Sciences (CNMS) Division, Physical Sciences Directorate (PSD) at Oak
Ridge National Laboratory (ORNL).

STEM of Heterostructured Quantum Materials: As a Postdoctoral Research
Associate, you will conduct research on epitaxial complex-oxide thin
films and heterostructures
as well as chalcogenide-based quantum materials using scanning
transmission electron microscopy/electron energy-loss
spectroscopy (STEM/EELS). This position is to lead our ongoing efforts
in understanding interfacial phenomena in functional
perovskite oxide materials for emerging information and energy
technologies as well as the area of quantum materials for
quantum information science. The work involves close collaboration
across groups to utilize advanced imaging and
high-resolution STEM/EELS to study thin film structures prepared by both
pulsed laser deposition (PLD) and molecular beam
epitaxy (MBE). This position will involve close collaboration with both
microscopists and film growers and will be using
advanced STEM facilities at ORNL.

Please apply directly via ORNL recruiting website.

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Oct 2018 14:21:20 -0500
Subject: [Microscopy] viaWWW:Difficulty obtaining Tokuyasu ultrathin sections of plant

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Email: samuel.livingston-at-botany.ubc.ca Name: Sam Livingston

Organization: University of British Columbia

Title-Subject: [Filtered] Difficulty obtaining Tokuyasu ultrathin
sections of plant material

Message: Dear Microscopy listserv,

I am having difficulty obtaining ultrathin sections of leaves and
flowers with the purpose of performing immunohistochemistry. My samples
were fixed in 4% PFA in TBS-T, infiltrated in an ascending grade of
sucrose up to 2.3 M, then embedded on a sectioning pin surrounded by 2.3
M sucrose by dipping into liquid nitrogen. I am sectioning on an
ultramicrotome equipped with a cryobox.

I'm finding that sectioning at 1 um thickness at -65 C has minimized the
amount of sucrose flaking, and is providing me with the best sections
for all conditions I have tried (from -60 to -90 at 5 C increments; 1.5
um to 0.3 um with .15 um increments). However, I am still experiencing
several issues:

The issues I'm having at 1 um sections; -65 C:
- sections are wrinkling and curling off the knife edge
- tissue sections do not adhere to slides after transferring out of cryobox
- tissue is largely fragmented upon imaging with TolBlue staining and
transmitted light, where epidermis, mesophyll and epidermal outgrowths
are floating all over the slide

My major concerns relate to the sections not adhering to my slides after
transfer, which will certainly wash off my slides during the
immunolabeling work up, and the fragmentation of my tissue, which
removes the spatial context needed for immunolabeling.

I have tried several different types of slides including uncoated,
pre-cleaned slides from Fisher, uncoated and non-cleaned slides from
VWR, as well as PTFE coated slides from EMS, with no apparent difference
in section adherence.
If you have any tips, tricks or recommendations please let me know, as I
would be very grateful for any help on this technically challenging and
patience testing method.

Sam Livingston

Login Host: 206.12.42.97
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



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21, 54 -- Subject: viaWWW:Difficulty obtaining Tokuyasu ultrathin sections of plant
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From: microscopy.listserver-at-gmail.com
Date: Sun, 21 Oct 2018 08:51:11 -0500
Subject: [Microscopy] viaWWW:Difficulty obtaining Tokuyasu ultrathin sections of plant

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-------- Forwarded Message --------


X-from: Rosemary.White-at-csiro.au



Hi Samuel,

You say you've tried several adhesives but have you tried chrom-alum, which works well in many
cases. It's relatively easy to make - there are many different recipes, e.g
http://stainsfile.info/StainsFile/prepare/adhesives/chromegelatin.htm. We resorted to this after
poly-lysine-, agar- and silane-coated slides - either purchased or prepared ourselves, proved
unreliable with a particularly difficult tissue.

I'm guessing the fragmentation of the tissue is because the cell walls are not sufficiently well
fixed/penetrated by the various solutions. Does happen with frozen plant tissues. For example,
rapid-frozen/freeze-substituted Arabidopsis roots have fantastically-preserved cell contents but the
cells tend to separate as you section the resin blocks. Tobias Baskin comments on this in one of his
earlier papers, I think.

good luck,
cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
Canberra, ACT 2601

T 61 2 6246 5475
M 61 468 966 713
________________________________________
X-from: microscopy.listserver-at-gmail.com [microscopy.listserver-at-gmail.com]
Sent: Thursday, 18 October 2018 6:21 a.m.
To: White, Rosemary (A&F, Black Mountain)




-------- Forwarded Message --------


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Email: samuel.livingston-at-botany.ubc.ca Name: Sam Livingston

Organization: University of British Columbia

Title-Subject: [Filtered] Difficulty obtaining Tokuyasu ultrathin
sections of plant material

Message: Dear Microscopy listserv,

I am having difficulty obtaining ultrathin sections of leaves and
flowers with the purpose of performing immunohistochemistry. My samples
were fixed in 4% PFA in TBS-T, infiltrated in an ascending grade of
sucrose up to 2.3 M, then embedded on a sectioning pin surrounded by 2.3
M sucrose by dipping into liquid nitrogen. I am sectioning on an
ultramicrotome equipped with a cryobox.

I'm finding that sectioning at 1 um thickness at -65 C has minimized the
amount of sucrose flaking, and is providing me with the best sections
for all conditions I have tried (from -60 to -90 at 5 C increments; 1.5
um to 0.3 um with .15 um increments). However, I am still experiencing
several issues:

The issues I'm having at 1 um sections; -65 C:
- sections are wrinkling and curling off the knife edge
- tissue sections do not adhere to slides after transferring out of cryobox
- tissue is largely fragmented upon imaging with TolBlue staining and
transmitted light, where epidermis, mesophyll and epidermal outgrowths
are floating all over the slide

My major concerns relate to the sections not adhering to my slides after
transfer, which will certainly wash off my slides during the
immunolabeling work up, and the fragmentation of my tissue, which
removes the spatial context needed for immunolabeling.

I have tried several different types of slides including uncoated,
pre-cleaned slides from Fisher, uncoated and non-cleaned slides from
VWR, as well as PTFE coated slides from EMS, with no apparent difference
in section adherence.
If you have any tips, tricks or recommendations please let me know, as I
would be very grateful for any help on this technically challenging and
patience testing method.

Sam Livingston

Login Host: 206.12.42.97
Listserver Email Form V - 20120416
---------------------------------------------------------------------------



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From: microscopy.listserver-at-gmail.com
Date: Mon, 22 Oct 2018 19:26:47 -0500
Subject: [Microscopy] viaWWW:Research Associate position in EPMA/SEM available at

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Email: zluo-at-uncfsu.edu Name: Zhiping Luo

Organization: Fayetteville State University

Title-Subject: [Filtered] Research Associate position in EPMA/SEM available at Fayetteville State
University

Message: A Research Associate regular staff position for EPMA/SEM is immediately available at FSU:

https://jobs.uncfsu.edu/postings/17614.

FSU houses an advanced JEOL JXA-8530F EPMA with a newly installed xCLent cathodoluminescence system.
More information can be found at https://www.uncfsu.edu/research/sencr-mic.


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From: microscopy.listserver-at-gmail.com
Date: Mon, 22 Oct 2018 19:27:42 -0500
Subject: [Microscopy] viaWWW: Help on Ted Pella Microwave oven

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] Ted Pella Microwave oven

Message: Hello. I am struggling to install new memory onto our Biowave. It won't read any of the
thumb drives we have tried. W tried reformatting, but nothing works. Has anyone else had this
problem? I am trying to restore the original settling that I erased by mistake.
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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 Oct 2018 18:16:52 -0500
Subject: [Microscopy] viaWWW:Imaging of pig pericardium

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Email: srousselle-at-alizeepathology.com Name: Serge Rousselle

Organization: Alizee Pathology, LLC

Title-Subject: [Filtered] Imaging of pig pericardium

Message: We are looking for a suitable technique to establish the 3D microstructure of a
decellularized sheet of swine pericardium (~15cmx8cmx0.5cm), with high enough resolution to
visualize the orientation and 3-D arrangement of collagen bundles and detect anomalies or inflection
points. The large size of the specimen makes it impractical for uCT. We were thinking of confocal
stereomicroscopy using collagen autofluorescence. We welcome practical suggestions. Thank you!

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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 Oct 2018 18:17:38 -0500
Subject: [Microscopy] viaWWW: used/salvage parts for JEOL SEM

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Email: dlowry-at-asu.edu Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] used/salvage parts for JEOL SEM

Message: I am looking for a source of used parts for a JEOL JSM-6300 or possibly 6400 series SEM,
specifically the video boards that control the CRTs. If anyone has a salvage unit or parts, or if
anyone knows of a potential parts source, please contact me off-line. thanks- Dave


David Lowry
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From: microscopy.listserver-at-gmail.com
Date: Sat, 27 Oct 2018 18:18:51 -0500
Subject: [Microscopy] viaWWW:FE-SEM gun lifetime

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Email: thoward-at-unm.edu Name: Tamara Howard

Organization: University of New Mexico

Title-Subject: [Filtered] FE-SEM gun lifetime

Message: I've sort of inherited oversight of a shared EM facility that includes a 2-year old FE-SEM.
Unfortunately, the system does not get much use, so I'd like to be able to shut down the gun when
there are no projects anticipated - it just costs too much for us to be able to replace the tip
every 12-16 months. The original one was swapped out at just under 24 months of age with less than
200 hours of actual use. It was still working & seemed to be stable, but was well over the suggested
run time.

I've been told by various people that there are facilities that routinely turn their FE-gun off when
not in use, but they did not know if anyone had standardized a re-start procedure. So, my question
is: would anyone who does this mind sharing how they manage this?
I will see replies to the ListServer; I'm having some issues with Outlook and could not post directly.

Thanks for your help!

Tamara




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From: Reinhard.Rachel-at-biologie.uni-regensburg.de
Date: Sun, 28 Oct 2018 05:49:32 -0500
Subject: [Microscopy] gun lifetime

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X-from: Guangming Cheng {gcheng-at-ncsu.edu}


Hi Tamara,
inheriting other microscopes can be a gift or a burden - who knows.
but in this case, a 2-yr old FE-SEM is - usually a wonderful gift.
The original gun was swapped out at just under 24 months of age ??? strange.
if it is an FE-gun, it can live 'for ever', if not heavily used. - In our FE-SEM (from 1999), we had one for far more than 5 years -- with little / occasional use (before I got it). What a shame for such an instrument. The gun was fine.
But, after 200 hrs, an FE-gun is not "well over the suggested run time" -- NO, NEVER.
A tungsten filament may be ... but even then: if it still gives nice images, it was treated well, and it can stay there. You write: " it was still working & seemed stable" - then, why changing? (Although, exchanging a W filament is not too tricky, and from the budget, not too heavy).
Basically, for all types of filaments, it is the usage AND the vacuum , which limits the lifetime. The level of vacuum which is required, is very different, for the different guns (W, LaB6, FE).
IF you really have an FE-SEM, then you may ask the manufacturer for an appropriate treatment during extended periods of "no use". And they also should have a procedure for a proper re-start . They should ...
Our FE-SEM and also our FE-TEM, both have one (and yes, for extended periods, I use the shut-down / re-start of the manufacturer). Works fine. - In our W-SEM and W-TEM, filaments are used until burnt.
kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29
Elected member of the IFSM board

Next microscopy conferences:
- Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin
- EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference)
- MC2021 in Vienna (D-A-CH conference)
- next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz





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From: microscopy.listserver-at-gmail.com
Date: Mon, 29 Oct 2018 07:27:49 -0500
Subject: [Microscopy] viaWWW:Bio-EM Senior Scientist: Electron Microscopist - TEM

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Title-Subject: [Filtered] Bio-EM Senior Scientist: Electron Microscopist - TEM

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From: microscopy.listserver-at-gmail.com
Date: Mon, 29 Oct 2018 07:28:36 -0500
Subject: [Microscopy] viaWWW:Biological Electron Microscopy Staff Scientist Position

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Message: The Analytical Instrumentation Facility (AIF) at NC State University is seeking an expert
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From: microscopy.listserver-at-gmail.com
Date: Mon, 29 Oct 2018 08:39:19 -0500
Subject: [Microscopy] gun lifetime

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X-from: Pappas, Richard Steve (CDC/DDNID/NCEH) {rlp6-at-cdc.gov}
To: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}

Hi Tamara,
inheriting other microscopes can be a gift or a burden - who knows. but in this case, a 2-yr old
FE-SEM is - usually a wonderful gift. The original gun was swapped out at just under 24 months of
age ??? strange. if it is an FE-gun, it can live 'for ever', if not heavily used. - In our FE-SEM
(from 1999), we had one for far more than 5 years -- with little / occasional use (before I got it).
What a shame for such an instrument. The gun was fine. But, after 200 hrs, an FE-gun is not "well
over the suggested run time" -- NO, NEVER. A tungsten filament may be ... but even then: if it
still gives nice images, it was treated well, and it can stay there. You write: " it was still
working & seemed stable" - then, why changing? (Although, exchanging a W filament is not too tricky,
and from the budget, not too heavy). Basically, for all types of filaments, it is the usage AND the
vacuum , which limits the lifetime. The level of vacuum which is required, is very different, for
the different guns (W, LaB6, FE). IF you really have an FE-SEM, then you may ask the manufacturer
for an appropriate treatment during extended periods of "no use". And they also should have a
procedure for a proper re-start . They should ...
Our FE-SEM and also our FE-TEM, both have one (and yes, for extended periods, I use the shut-down /
re-start of the manufacturer). Works fine. - In our W-SEM and W-TEM, filaments are used until burnt.
kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29
Elected member of the IFSM board

Next microscopy conferences:
- Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin
- EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference)
- MC2021 in Vienna (D-A-CH conference)
- next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz


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From: jrminter-at-gmail.com
Date: Mon, 29 Oct 2018 12:47:54 -0500
Subject: [Microscopy] Re: FEG tip lifetime

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Tamara,

I agree with Reinhard. The specifics depend upon the type of FEG that
you have. In general FEG microscopes require a high vacuum. The SEM
and FIB in my lab had Schottky Field Emission guns. Like all FEGs,
they required very high vacuums to work. The tip was always on. If
your microscope is like this, you may use the microscope for only a
few hours per month, but the tip is running all the time...

A complete shutdown of our FEG SEM and FIB for more than a day or so
required a bake-out procedure to bring it back up. This could take a
day or two to get the microscope back to working order. Like
Reinhard's microscope, our microscopes had a "standby" mode where the
gun was maintained under vacuum with the ion getters on and could be
restarted easily and the tip turned back on in a few hours. I never
tried using this mode for longer than a week or two. We typically
needed to replace the tip every couple of years, but our microscopes
were used daily. I recommend you contact the manufacturer and ask for
their best practices for your microscope and use case.

Best regards,
John Minter
Retired from Kodak Analytical Sciences

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From: microscopy.listserver-at-gmail.com
Date: Mon, 29 Oct 2018 19:45:51 -0500
Subject: [Microscopy] viaWWW:job opportunity - Director Cellular Imaging at Fred Hutch

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Email: jvazquez-at-fredhutch.org Name: Julio Vazquez

Organization: Fred Hutch Cancer Center

Title-Subject: [Filtered] job opportunity - Director Cellular Imaging at Fred Hutch

Message: Dear Microscopists,

Fred Hutch Cancer Center in Seattle has an opening for the position of Director of Cellular Imaging.
The Cellular Imaging Core provides an extensive array of light and electron microscopy
instrumentation and services to support research by Fred Hutch, University of Washington, and
Seattle Children's Cancer Consortium researchers, as well as other local institutions and biotechs.
The Core is currently expanding its capabilities in super-resolution and planning to bring in new
capabilities including light sheet microscopy and Cryo-EM. Fed Hutch is located in the Seattle South
Lake Union neighborhood, in the heart of a vibrant research and biotech community.

https://careers-fhcrc.icims.com/jobs/11530/morphometry-and-image-analyst/job?hub=7&mobile=false&width=960&height=500&bga=true&needsRedirect=false&jan1offset=-480&jun1offset=-420

More details about the Cellular imaging Core can be found here:

https://sharedresources.fredhutch.org/core-facilities/cellular-imaging

Please bring this to the attention of any colleagues who might be interested. Thank you!

Julio Vazquez
Director of Cellular Imaging
Fred Hutch Cancer Research Center
Seattle, WA 98119



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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Oct 2018 07:29:37 -0500
Subject: [Microscopy] viaWWW: MNT-JS11600 sputter coater

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Email: pscallio-at-dal.ca Name: Patricia Scallion

Organization: Dalhousie University

Title-Subject: [Filtered] MNT-JS11600 sputter coater

Message: Hello,

I was wondering if anyone here has experience or comments on the MNT-JS11600 plasma sputter coater
from MicroNano Tools?

Replies can be on the Listserver, or you can PM me.

Regards,
Pat Scallion

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From: microscopy.listserver-at-gmail.com
Date: Wed, 31 Oct 2018 07:42:44 -0500
Subject: [External] [Microscopy] viaWWW:HPM100 services

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Nessler, Randy A {randy-nessler-at-uiowa.edu}


Hi Naomi,
I have had Bill service our HPF before. His contact info is:

Bill Graham
BIBST LABS
37 Averill Road
BROOKLINE, NH 03033

603-345-3887
BILL-at-BIBST.COM

Regards,
Randy

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Sent: Thursday, May 17, 2018 2:03 PM
To: Nessler, Randy A




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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] HPM100 services

Message: Hello,

I wonder if there are any 3rd party companies/persons who are providing
services on the high-pressure freezer, HPM100.

Any information would be appreciated.

Thank you,

Naomi Kamasawa
Head, Electron Microscopy Facility
Max Planck Florida Institute for Neuroscience
One Max Planck Way
Jupiter, FL 33458 USA


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From: yuanyuanzhu2014-at-gmail.com
Date: Wed, 31 Oct 2018 15:29:28 -0500
Subject: [Microscopy] In-situ (S)TEM postdoc position at UConn

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Job Posting Title: Postdoctoral Research Associate Position on In-situ (Scanning) Transmission Electron Microscopy

The University of Connecticut (UConn), one of the top 20 public universities in the nation, invites applications for a Postdoctoral Research Associate position in the Department of Materials Science and Engineering (MSE) in the School of Engineering and the Institute of Materials Science (IMS). Applicants with a strong background in aberration-corrected (scanning) transmission electron microscopy ((S)TEM) and associated analytical techniques are encouraged to apply. In this position, you will have the opportunity to engage creative research on investigating structure-property correlations in advanced functional and structural materials including but not limited to heterogeneous catalysts. You will have the opportunity to work in a state-of-the-art TEM center hosting a probe-corrected Titan Themis STEM, and perform microscopy in the area of in-situ (gaseous) environmental (S)TEM, including new capability development.
UConn is entering a transformational period of growth supported by the $1.7B Next Generation Connecticut (http://nextgenct.uconn.edu/) and the $1B Bioscience Connecticut (http://biosciencect.uchc.edu/) investments and a bold new Academic Plan: Path to Excellence (http://issuu.com/uconnprovost/docs/academic-plan-single-hi-optimized_1). As part of these initiatives, the new UConn-Thermo Fisher Scientific Center for Advanced Microscopy and Materials Analysis (CAMMA) has acquired seven new electron beam instruments including state-of-the-art TEM, SEM and FIB systems. These are housed in the UConn Technology Park as part of the purpose-built Advanced Characterization Laboratory, which opened earlier this year.

DUTIES AND RESPONSIBILITIES
The successful candidate will share a deep commitment to transmission electron microscopy. Working under the supervision of Dr. Yuanyuan Zhu, the candidate will be expected to lead microscopy researches to further the understanding of materials dynamics. The candidate will also contribute to TEM sample preparation, mentor graduate and undergraduate students; write research proposals and progress reports; interact with research collaborators; prepare and maintain lab equipment and supplies, submit and publish peer reviewed journal papers.

MINIMUM QUALIFICATIONS
An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A strong background and extensive research experience in TEM sample preparation (e.g. FIB liftout) and (scanning) transmission electron microscopy characterization. Good written and verbal communication skills. Good research capabilities as evidenced by a record of publication of results in peer-reviewed journals and external presentations at scientific conferences.

PREFERRED QUALIFICATIONS
Additionally, the candidate is expected to be proficient at three or more of the following techniques including but not limited to: TEM Tilt-Series, SAED, Nanobeam Electron Diffraction, image-corrected HRTEM, probe-corrected STEM, EDS mapping, core- (and low-) loss EELS, in-situ heating TEM. Skills and experience in in-situ gas-cell microscopy is highly desired. Strong interpersonal skills including the ability to interact effectively with staffs, students and collaborators.

APPOINTMENT TERMS
The selected candidate is expected to start in January 2019, or earlier if reach a mutual agreement. This is a full-time (12-month appointment) position, and is renewable every year. The successful candidates primary academic appointment will be at the UConn main campus in Storrs, CT. Salary will commensurate with qualifications and experience.

TO APPLY
Please submit the following: acover letter;curriculum vitae (with a full list of publication), copies of two representative publicationstomailto:yuanyuan.2.zhu-at-uconn.edu , with a subject title In-situTEMPostdoc_yourname.
Evaluation of applicants will begin immediately and continue until the position is filled. Employment of the successful candidate will be contingent upon the successful completion of a pre-employment criminal background check.
All employees are subject to adherence to the State Code of Ethics, which may be found athttp://www.ct.gov/ethics/site/default.asp.
____________________________________________________________________
The University of Connecticut is committed to building and supporting a multicultural and diverse community of students, faculty, and staff. The diversity of students, faculty, and staff continues to increase, as does the number of honors students, valedictorians and salutatorians who consistently make UConn their top choice. More than 100 research centers and institutes serve the Universitys teaching, research, diversity, and outreach missions, leading to UConns ranking as one of the nations top research universities. UConns faculty and staff are the critical link to fostering and expanding our vibrant, multicultural, and diverse community. As an Affirmative Action/Equal Employment Opportunity employer, UConn encourages applications from women, veterans, people with disabilities, and members of traditionally underrepresented populations.



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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Nov 2018 13:45:55 -0500
Subject: [Microscopy] viaWWW: tannic acid - looking for a reference

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-------- Forwarded Message --------

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: The George Washington University

Title-Subject: [Filtered] tannic acid-looking for a reference

Message: Good morning,

I am looking for a reference about using tannic acid as a tracer for damaged plasma membranes. Can
anyone help me with this reference from an old journal? Or a different, more easily obtainable
reference for the procedure?

Stain Technol. 1980 Nov;55(6):361-5.
Tannic acid as an electron microscope tracer for permeable cell membranes.

Nnez-Durn H.


Thank you
Chris Brantner
George Washington University Nanofabrication and Imaging Center

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From: microscopy.listserver-at-gmail.com
Date: Thu, 1 Nov 2018 17:22:35 -0500
Subject: [Microscopy] viaWWW: MNT-JS11600 sputter coater

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-------- Forwarded Message --------

X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

It looks like a very basic instrument - but maybe that is what you are looking for. The price is
less than what other vendors offer.
The company also looks to be fairly new. That could mean that they are more aggressive in their
pricing. I hope their engineering is good and that they will stand behind their work.
They list options for multiple metals. That is good, because gold is not good for high magnification
work (e.g., 50kx); you will see the structure of the deposited gold film. (We purchased an iridium
coater when we got our FE-SEM.) I don't know what voltages is needed for the different metals. It
seems they are limited to no more than 1600V. I can't tell if it is adjustable.
Warren Straszheim, manager
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday,
October 31, 2018 7:30 AM
To: Straszheim, Warren E [BIOTC]




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Email: pscallio-at-dal.ca Name: Patricia Scallion

Organization: Dalhousie University

Title-Subject: [Filtered] MNT-JS11600 sputter coater

Message: Hello,

I was wondering if anyone here has experience or comments on the MNT-JS11600 plasma sputter coater
from MicroNano Tools?

Replies can be on the Listserver, or you can PM me.

Regards,
Pat Scallion

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From: microscopy.listserver-at-gmail.com
Date: Sun, 11 Nov 2018 10:51:53 -0600
Subject: [Microscopy] viaWWW:Position for microscopist / optical engineer

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Email: khahn-at-med.unc.edu Name: Klaus Hahn

Organization: UNC - Chapel Hill

Title-Subject: [Filtered] Position for microscopist / optical engineer

Message: Staff scientist at UNC-Chapel Hill: Microscopist / Optics engineer

We are looking for a person with a strong background in microscopy and/or optical engineering to
develop new microscopes for live cell imaging. The Hahn lab designs proteins and small molecules to
visualize and control signaling (see hahnlab.com) and is now working closely with engineering and
computational biology groups to engineer new microscopes and harness capabilities of novel proteins
and dyes (eg Legant, Superfine, Elston groups at UNC). You will assemble and focus on microscopes
with diverse capabilities, including extensions of single particle tracking, PALM/STORM, SIM-TIRF,
lattice light sheet and spin TIRF. In addition to optics, experience in tissue culture,
biosensors/optogenetics, or cell biology would be a plus. The candidate should be familiar with at
least one of the following programming languages: MATLAB, LabVIEW, Python, Java, R, etc. Experience
with Metamorph and Micromanager/ImageJ. A PhD is preferred and a minimum of a bachelors degree with
relevant experience is required. Please send your CV and cover letter s to Klaus Hahn,
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From: thomas.danz-at-uni-goettingen.de
Date: Tue, 13 Nov 2018 12:03:40 -0600
Subject: [Microscopy] Have a JEOL 2100F TEM with high-tilt pole piece?

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

my colleague Tyler emailed the list about a year ago asking for peoples'
experience with the JEOL 2100F high-tilt (HT) pole piece. We got one
really helpful response from someone with a similar instrument. We now
have a more basic question: do you have a JEOL 2100F with a high-tilt
pole piece and do you mind if we bother you with a few quick questions
about the performance of your microscope? (If you have a 2010F with a
high-tilt pole piece, we'd also be interested to talk to you.) It seems
that this is a relatively rare pole piece.

Thanks,
Thomas

--
Thomas Danz
IV. Physikalisches Institut
Georg-August-Universität Göttingen
Friedrich-Hund-Platz 1
D-37077 Göttingen

Email: thomas.danz-at-uni-goettingen.de
Tel: +49 551 39 4557


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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Nov 2018 06:55:09 -0600
Subject: [Microscopy] viaWWW:Denton DV-502A manual needed

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Email: dp.ssize-at-comcast.net Name: D Palacio

Title-Subject: [Filtered] Denton DV-502A manual needed

Message: Hi listers,

Does anyone have a manual for the Denton DV-502A vacuum evaporator or can direct me where I might
find one?

Thank you!
D. Palacio
dp.ssize-at-comcast.net

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From: microscopy.listserver-at-gmail.com
Date: Sat, 17 Nov 2018 09:51:33 -0600
Subject: [Microscopy] viaWWW:Features function AZtec 3.3 and up

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Email: nikd-at-vsl.cua.edu Name: Nik Deems

Organization: Vitreous State Laboratory

Title-Subject: [Filtered] Features function AZtec 3.3 and up

Message: Hi all,

My colleagues and I just upgraded our EDS and our software to AZtec 3.3. We are trying to make a go
at the Features function and are having some difficulty doing what we need to do (basically find the
percentage of the whole of the image of a set of features based on contrast threshold).

If anyone if familiar with this function, could you please e-mail me?

Cheers,
Nik Deems

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From: microscopy.listserver-at-gmail.com
Date: Mon, 19 Nov 2018 07:34:44 -0600
Subject: [Microscopy] viaWWW:automatic contrasting system for ultrathin sections

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Email: agnes.matysiak-at-pathologie-bochum.de Name: Agnes Matysiak

Title-Subject: [Filtered] automatic contrasting system for ultrathin sections

Message: Hello all,

our 25 years old automatic contrasting system is not working any more. Do you have a recommendation
/ good experience with a new instrument? I am thinking about the Leica AC20. But I was wondering if
there are other companies than Leica which sell an instrument for contrasting and provide service in
Germany? Thank you very much for your help!
Best wishes, Agnes Matysiak
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From: Aleksandr.Mironov-at-manchester.ac.uk
Date: Mon, 19 Nov 2018 09:46:37 -0600
Subject: [Microscopy] TEM: T12 replacement

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

We are Core Facility doing mostly biology TEM. Our 2 Tecnai 12s are
getting old and at some point I amafraid we will not be able to find
computer boards for failed obsolete old ones. So, we are thinking to
replace at least one of them with some modern alternative. As we are
mostly doing bright field standard TEM with plasticembedded samples or
negatively stained biology materials then the choice goes to 3 options:
FEI Talos 120, Jeol 1400Flash or Hitachi 7800 series. We rarely do STEM
but it could be of benefit to have this option. I would like to have
some feedback on these instrumentsregarding value for money, how robust
they are,how good and fast service is in your local area, how good user
interface is, how steep is a learning curvefor an inexperienced user and
will it be worthwhile to learn new interface if we will move away from FEI.

We need just workhorse instrument that will not break often and will be
simple to use. Also I am interested in your opinion of how useful some
particular features for specific instruments:

1) Hitachi HT7800 series:

        - How good Dual-Mode objective lens is? Is it worthwhile?

2) Jeol 1400Flash:

        - How good is OM image linkage function? What it is doing exactly?

3) I have a feeling that FEI Talos is a bit overpriced in comparison to
its rivals. Is it correct?


I would really appreciate any insights about real life issues and
experiences with these instruments.

Please, reply to my UK e-mail address to avoid any collisions on the list.

Sincerely,

Alex

--
Dr. Aleksandr Mironov MD, PhD
Senior Experimental Officer
D.1527, M.Smith Building
EM Core Facility
School of Biological Sciences,
Faculty of Biology Medicine and Health
University of Manchester
Oxford Road
Manchester
M13 9PT
UK


E-mail: Aleksandr.Mironov-at-manchester.ac.uk
https://www.bmh.manchester.ac.uk/research/facilities/electron-microscopy/


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From: John.Mardinly-at-asu.edu
Date: Sun, 25 Nov 2018 15:15:24 -0600
Subject: [Microscopy] Aaron Klug, Nobel-winning scientist who examined crystal structure,

Contents Retrieved from Microscopy Listserver Archives
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A. John Mardinly, Ph.D., P.E.


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4, 101 -- Subject: Aaron Klug, Nobel-winning scientist who examined crystal structure,
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From: mabon-at-illinois.edu
Date: Wed, 28 Nov 2018 09:22:00 -0600
Subject: [Microscopy] Position Available: Bio / Soft Materials EM Research Scientist -

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Research Scientist
Materials Research Laboratory
University of Illinois at Urbana-Champaign

The Materials Research Laboratory (MRL) at the University of Illinois at Urbana-Champaign is seeking a highly motivated Research Scientist to work in its Electron Microscopy instrumentation core facility. The Research Scientist will serve as a member of the MRL Central Research Facilities staff with a focus on enabling soft materials and biological imaging research by training and advising users in electron microscopy and related methods (including specimen preparation), and by engaging in collaboration with various local and regional users on active research projects.
The individual's job responsibilities include:

* Supporting research on soft materials and biological imaging in MRL by serving as the technical point of contact to the research community. Activities include managing user interactions, developing research proposals, and assisting with data interpretation.
* Developing expertise in the instruments and techniques by remaining abreast of advances in the field to advance the local research community's proficiency in the techniques. Activities in this area include ongoing review of relevant scientific literature, participation in professional societies and conference/workshop/training opportunities, and advising facilities leadership on emerging instrumentation needs.
* Training and advising users in electron microscopy and related methods, including preparation of soft materials and biological samples for electron imaging. Activities beyond hands-on training include developing and improving training materials as needed (based on developments in the field, maintenance or service alerts, and user feedback), and engaging in collaborative research projects with various local and regional users by providing direct support in electron imaging and assistance in data interpretation.
* Coordinating instrument updates and/or major repairs with manufacturers/vendors, other support personnel and facilities leadership as appropriate. Specific activities include: assuring that instrumentation is kept in optimum working condition by actively monitoring its usage and performance against established metrics; developing standard schedules for routine maintenance based upon manufacturer documentation, use patterns and facilities best practices; performing routine maintenance in conjunction with other supporting staff; and troubleshooting the root cause of suboptimal performance and making minor repairs as needed.
* Assisting with the MRL's outreach activities, including participating in the development of new research initiatives and providing improvements to existing outreach programs.
* Performing additional research scientist duties to promote the unit's mission as needed.

The successful candidate should demonstrate excellent verbal and written communication skills and must be able to work independently and proactively with multiple users. The candidate must have a Bachelor's degree or higher in physical or life sciences or engineering. The candidate must have one to three years of demonstrated, hands-on experience with electron microscopy. Preference will be given to candidates with a Master's degree, Ph. D, or a Bachelor's degree and three or more years of demonstrated, hands-on experience with biological or soft materials electron microscopy, including handling of biohazardous materials. Experience with cryo-electron microscopy instrumentation and techniques, including cryo-ultramicrotomy and cryo-plunge, and image analysis is preferred.

This is a full-time, benefits-eligible academic professional position appointed on a 12-month service basis. The expected start date is as soon as possible. Salary is commensurate with experience and qualifications. To apply, please complete a candidate profile at http://jobs.illinois.edu {http://jobs.illinois.edu/} and upload the following as a single file: a cover letter, resume, and the names and contact information for three professional references. To ensure full consideration, all requested information must be submitted by December 15, 2018. Applicants may be interviewed before the closing date; however, no hiring decision will be made until after that date.

The successful candidate will become a member of the dedicated staff of about 20 scientists and engineers, who maintain major research instruments in the MRL's core facilities for soft materials characterization, scanning probe microscopy, laser and optical spectroscopy, electron microscopy, surface analysis, x-ray diffraction, and nanofabrication facilities including two cleanrooms. Over 1,000 researchers from across our campus as well as other academic institutions, industry, and national laboratories use the facilities, logging more than 100,000 user hours annually. The lab is recognized as one of the premier mid-sized user facilities in the nation. For details, please visit us online at https://mrl.illinois.edu/facilities/.

The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer.

The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women, veterans and individuals with disabilities are encouraged to apply. For more information, visit http://go.illinois.edu/EEO. To learn more about the University's commitment to diversity, please visit http://www.inclusiveillinois.illinois.edu


Thanks,

Mauro
___________________________________
Mauro Sardela, Ph.D.
Director, Central Research Facilities
Materials Research Laboratory
University of Illinois at Urbana-Champaign
104 S. Goodwin Avenue - Urbana IL 61801
(217) 244 0547 | office # SC2014
www.mrl.illinois.edu {http://www.mrl.illinois.edu/




_______________________________________________


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16, 81 -- Subject: Position Available: Bio / Soft Materials EM Research Scientist -
16, 81 -- University of Illinois at Urbana - Champaign
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16, 81 -- - University of Illinois at Urbana - Champaign
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From: gilpin-at-purdue.edu
Date: Wed, 28 Nov 2018 12:14:34 -0600
Subject: [Microscopy] picking up sections from something other than water etc

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Everyone,
I have a user cutting thin sections of a polymer that is soluble in water, methanol, ethanol and other organic solvents.

Any thoughts about alternatives liquids?

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Campus-wide Coordinator for Electron Microscopy
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
skype cjgilpin




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7, 32 -- Subject: picking up sections from something other than water etc
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From: vray-at-partbeamsystech.com
Date: Wed, 28 Nov 2018 12:54:40 -0600
Subject: [Microscopy] Re: picking up sections from something other than water

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

How about fully fluorinated liquids, such as Galden by Inland Vacuum?
Another one of chemically inert variants, such as Perfluorodecalin maybe?

Valery Ray (also with REFINE/UCONN)
===================================
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
MEO Engineering / PBS&T
290 Broadway, Suite 298
Methuen, MA 01844, USA
www.partbeamsystech.com
www.freudlabs.com

On 11/28/2018 1:14 PM, gilpin-at-purdue.edu wrote:
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} Hi Everyone,
} I have a user cutting thin sections of a polymer that is soluble in water, methanol, ethanol and other organic solvents.
}
} Any thoughts about alternatives liquids?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
} Campus-wide Coordinator for Electron Microscopy
} Director, Life Science Microscopy Facility
} Purdue University
} Whistler Hall of Agriculture Research, Room S052
} 170 S. University St
} West Lafayette, IN 47907
} 765-494-7750
} gilpin-at-purdue.edu
} lsmf-at-purdue.edu reaches everyone in the facility.
} http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
} skype cjgilpin
}
}
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From: acoritz-at-emsdiasum.com
Date: Wed, 28 Nov 2018 15:30:31 -0600
Subject: [Microscopy] Re: picking up sections from something other than water

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Valery:

Not so sure these would be compatible with Diamond knives and their mounting cements.

Best to ask Helmut Gnaegi of Diatome for ideas how to do this Chris
helmut.gnaegi-at-diatome.ch

Al Coritz
Electron Microscopy Sciences & Diatome US

-----Original Message-----
X-from: vray-at-partbeamsystech.com {vray-at-partbeamsystech.com}
Sent: Wednesday, November 28, 2018 1:59 PM
To: Al Coritz {acoritz-at-emsdiasum.com}

How about fully fluorinated liquids, such as Galden by Inland Vacuum?
Another one of chemically inert variants, such as Perfluorodecalin maybe?

Valery Ray (also with REFINE/UCONN)
===================================
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
MEO Engineering / PBS&T
290 Broadway, Suite 298
Methuen, MA 01844, USA
www.partbeamsystech.com
www.freudlabs.com

On 11/28/2018 1:14 PM, gilpin-at-purdue.edu wrote:
} ----------------------------------------------------------------------
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}
} Hi Everyone,
} I have a user cutting thin sections of a polymer that is soluble in water, methanol, ethanol and other organic solvents.
}
} Any thoughts about alternatives liquids?
}
} ​​​​​
} Chris
}
} Christopher J. Gilpin Ph.D.
} Campus-wide Coordinator for Electron Microscopy Director, Life Science
} Microscopy Facility Purdue University Whistler Hall of Agriculture
} Research, Room S052
} 170 S. University St
} West Lafayette, IN 47907
} 765-494-7750
} gilpin-at-purdue.edu
} lsmf-at-purdue.edu reaches everyone in the facility.
} http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
} skype cjgilpin
}
}
}
}
} ==============================Original
} Headers==============================
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From: Rosemary.White-at-csiro.au
Date: Wed, 28 Nov 2018 16:22:46 -0600
Subject: [Microscopy] experimental scientist (microscopist) position in Canberra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

The following position is open until Tuesday, 18 December.

Experimental Scientist - Black Mountain MicroImaging Centre (BMIC)

If interested, please find further information and apply ONLINE:
https://jobs.csiro.au/job/Canberra%2C-ACT-Experimental-Scientist-Black-Mountain-MicroImaging-Centre-%28BMIC%29/520162300/?locale=en_GB
Please do not reply to this email.

The Black Mountain MicroImaging Centre (BMIC) is a state-of-the-art Centre which builds and fosters human and scientific capabilities, to produce innovative cross-discipline research and deliver impact in crop science. BMIC is equipped with general PC2 lab facilities, confocal, fluorescent, scanning electron, dissecting and brightfield microscopes, embedding and sectioning equipment and Fourier Transform Infra-Red spectroscope/microscope.

Your duties will include
Under the general direction of the BMIC manager, participate in planning of the microscopy component of a wide range of projects focussed on crops and other plants, with responsibility for the scheduling and completion of project work, including allocating and directing tasks where appropriate and documentation of methods and procedures.
Provide timely, accurate and professional advice to staff about structural and developmental aspects of their research.
Adapt and/or develop original experimental methods, concepts and ideas for the imaging component of existing and further research, promptly addressing where methods may not be defined. Initiative is required in seeking new approaches to meet experimental objectives.
Develop high-level expertise in various types of microscopy, and/or digital image analysis and image management.
Make significant contributions to the interpretation and communication, both internal and external, of research results and collaboration on drafting and delivering presentations to, and/or detailed written reports for, clients and the scientific and technology community.
A significant component of this position is to provide on-the-job training and instruction in microscopy, imaging, and other relevant instruments and techniques to colleagues, including visitors, students and postdocs, and managing resources to meet objectives, as required.
Ensure the smooth operation of BMIC by carrying out the role of lab custodian. This includes maintaining adequate levels of consumables, keeping the laboratories tidy, assisting in upkeep of specialised equipment and software, conducting regular PC2 audits and promoting best HSE practices in BMIC.

Salary: $AUD 82,450 to $SAUD 93,280 plus up to 15.4% Superannuation
Tenure: Indefinite

Essential Criteria:
Demonstrated knowledge of, and experience in analysis of plant structure and functional plant anatomy.
Demonstrated experience in specimen preparation for light and electron microscopy and in use of light, fluorescence, confocal and electron microscopes.
Experience in digital imaging and image analysis.
Proven ability to work with minimum supervision, both independently and as part of a team, on a variety of research topics.
Demonstrated ability to use initiative in solving technical and research problems.
Demonstrated ability to organise data and resources, and to work to defined timelines and milestones.
Excellent written, oral and interpersonal communication skills, including the ability to liaise with staff at all levels with professionalism. This includes the ability to train staff and students on the use of specialized equipment as well as provide basic guidance in the design of experiments using these instruments.

Desirable Criteria:
Experience in presentation of results at conferences and in publication of scientific papers.
Experience in cross-program and cross-institution research collaborations.
Demonstrated knowledge and experience in the specialist areas of image analysis, including deconvolution and 3D reconstruction will be considered highly desirable.
Experience in resin-embedding, sectioning and immunostaining of plant tissues for light and electron microscopy.
Experience in lab management.


cheers,
Rosemary


Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
Canberra, ACT 2601
Australia

T 61 2 6246 5475
M 61 468 966 713

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From: microscopy.listserver-at-gmail.com
Date: Wed, 28 Nov 2018 18:25:37 -0600
Subject: [Microscopy] Re: viaWWW:Imaging of pig pericardium

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-------- Forwarded Message --------


X-from: Stephane Nizet {nizets2-at-yahoo.com}


Dear Serge,

sorry for replying late.
I would simply do histology with serial sectionning. staining collagen in histology is standard,
most histology labs should be able to do it.
You probably don't need the whole piece of tissue to determine the orientation of the fibers.

Best regards,
Stephane
On Sunday, October 28, 2018, 1:26:36 AM GMT+2, microscopy.listserver-at-gmail.com
{microscopy.listserver-at-gmail.com} wrote:





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Email: srousselle-at-alizeepathology.com {mailto:srousselle-at-alizeepathology.com} Name: Serge Rousselle

Organization: Alizee Pathology, LLC

Title-Subject: [Filtered] Imaging of pig pericardium

Message: We are looking for a suitable technique to establish the 3D microstructure of a
decellularized sheet of swine pericardium (~15cmx8cmx0.5cm), with high enough resolution to
visualize the orientation and 3-D arrangement of collagen bundles and detect anomalies or inflection
points. The large size of the specimen makes it impractical for uCT. We were thinking of confocal
stereomicroscopy using collagen autofluorescence. We welcome practical suggestions. Thank you!

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From: microscopy.listserver-at-gmail.com
Date: Thu, 29 Nov 2018 17:23:16 -0600
Subject: [Microscopy] viaWWW:EMS Microscopy Academy Workshops

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Organization: Electron Microscopy Sciences

Title-Subject: [Filtered] EMS Microscopy Academy Workshops

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From: microscopy.listserver-at-gmail.com
Date: Fri, 30 Nov 2018 08:31:18 -0600
Subject: [Microscopy] viaWWW: Bio TEM Workshop

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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Bio TEM Workshop

Message: This intensive, three-day workshop provides a practical and basic theoretical introduction
to the Transmission Electron Microscope and biological sample preparation techniques. Each day will
consist of lecture, discussion and *hands-on* training led by GEM staff.
********
Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment. When: Wednesday through Friday,
March 13-15, 2019, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: March 4, 2019


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From: tbargar-at-unmc.edu
Date: Fri, 30 Nov 2018 10:16:41 -0600
Subject: [Microscopy] calibrating monitor and printer for image output

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

Is there a way to calibrate your computer monitor and printer? When I print images what I see on my monitor will come out of the printer slightly darker, colors will be off from what I have in the image as displayed on the monitor. As always all help is appreciated and Thanks.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Dec 2018 20:28:52 -0600
Subject: [Microscopy] viaWWW:European EELS & EFTEM School at Graz University

Contents Retrieved from Microscopy Listserver Archives
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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] European EELS & EFTEM School at Graz University

Message: European EELS & EFTEM School at Graz University

The joint FELMI/European EELS & EFTEM School is an intense four-day, hands-on laboratory workshop
that will take you step-by-step through the acquisition and analysis of EFTEM and STEM EELS data.
The workshop will utilize the state of the art facilities at FELMI-ZFE including a monochromated
probe-corrected Titan (S)TEM with a DualEELS GIF Quantum EELS system. The EELS spectrometer features
a unique direct-electron detection K2 camera for low-noise, dose efficient applications in imaging
and spectroscopy.

Our qualified staff will familiarize you with the latest EELS & EFTEM equipment and teach you
fundamental principles and methods that are crucial to take top quality EELS spectra, STEM EELS
spectrum images, energy-filtered images, or elemental maps. While not a focus of the workshop, the
program will include optimization of the source monochromator for high-resolution EELS and the Cs
probe corrector for STEM EELS.

For complete details and to register, please visit the Graz Life Long Learning Courses website at:
https://www.felmi-zfe.at/teaching/lll-courses/


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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Dec 2018 20:29:51 -0600
Subject: [Microscopy] viaWWW:EELS & EFTEM Analysis Training School 2019

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School 2019

Message: Electron energy loss spectroscopy (EELS) is a powerful technique that provides both
compositional and chemical information from sub-nanometer areas in the sample. As a course attendee,
you will learn best practices to set up and optimize your EELS hardware and experimental protocols
so you can capture and extract the maximum amount of compositional and chemical information from
your TEM samples. Topics include:

Fundamentals of EELS and energy-filtered imaging in TEM
Principles of operation of Gatan EFTEM and EELS systems
Optimization of EFTEM and EELS data acquisition
Quantification of elemental composition
Other information provided by EFTEM/EELS and how best to extract it
Use of EELS signals to form maps of elemental and chemical composition
EFTEM and STEM EELS spectrum imaging techniques
Identification of material phases via EELS fine structure mapping
Applications to biological and physical science specimens

For more details and online registration, please visit:
http://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2019



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From: frank_karl-at-ardl.com
Date: Wed, 5 Dec 2018 10:05:05 -0600
Subject: [Microscopy] Screen lifter blues

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Hello Everyone,
Merry Christmas.

My Philips CM12 TEM has a tendency to shut off if I lift the focus screen too fast (too hard?) to expose the bottom mount camera. This is relatively new to me, as I was using a side mount camera and never had to lift the plate.

Is this normal?

If it's not, other than being slow and delicate, what was the cure?


Oh, yes, if you don't celebrate Christmas, that fine. I wish you the very best at this time of year.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



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From: vitaly-at-sia-cam.com
Date: Wed, 5 Dec 2018 10:48:11 -0600
Subject: [Microscopy] Re: Screen lifter blues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Disassemble feed-through shaft assembly, clean it and replace O-rings.

Viewing windows O-rings may need replacement too unless  done already.
Ditto large screen assembly.

Most O-rings around projection chamber are Buna-N rubber. Install Viton
O-rings instead, these last forever more or less.


Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 12/5/2018 11:09 AM, frank_karl-at-ardl.com wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello Everyone,
} Merry Christmas.
}
} My Philips CM12 TEM has a tendency to shut off if I lift the focus screen too fast (too hard?) to expose the bottom mount camera. This is relatively new to me, as I was using a side mount camera and never had to lift the plate.
}
} Is this normal?
}
} If it's not, other than being slow and delicate, what was the cure?
}
}
} Oh, yes, if you don't celebrate Christmas, that fine. I wish you the very best at this time of year.
}
}
}
}
} Stay safe...........
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}
}
} ==============================Original Headers==============================
} 13, 66 -- From frank_karl-at-ardl.com Wed Dec 5 10:05:04 2018
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} 13, 66 -- Subject: Screen lifter blues
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==============================Original Headers==============================
7, 43 -- From vitaly-at-sia-cam.com Wed Dec 5 10:48:10 2018
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7, 43 -- id 1gUaPd-0005Hd-2J; Wed, 05 Dec 2018 11:52:37 -0500
7, 43 -- Subject: Re: [Microscopy] Screen lifter blues
7, 43 -- To: frank_karl-at-ardl.com, Microscopy Listserver {Microscopy-at-microscopy.com}
7, 43 -- References: {201812051609.wB5G9PGY013637-at-microscopy.com}
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From: Reinhard.Rachel-at-biologie.uni-regensburg.de
Date: Wed, 5 Dec 2018 10:51:29 -0600
Subject: [Microscopy] Screen lifter blues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

} } } 05.12.2018 at 17:06:
} My Philips CM12 TEM has a tendency to shut off if I lift the focus screen too
} fast (too hard?) to expose the bottom mount camera. This is relatively new
} to me, as I was using a side mount camera and never had to lift the plate.

Dear Frank,
have a CM12 since 1990 ( oh yes, I remember it VERY WELL - the boxes were delivered on Dec 4th, 1990, two days before St. Nicklas day ...) and it is still in use.
I had quite a few pecularities, but never heard of this one. No, this is NOT normal ... for sure.
We have a bottom-mount camera in operation (TVIPS) since 1998, daily, heavy used - no problems with any of the phosphor screens ... and I keep my fingers crossed that we will never have a problem like this.
I only hope that any of the many users out there can help ...
kind regards, have a nice December, merry Xmas and happy new year!
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29
Elected member of the IFSM board

Next microscopy conferences:
- Microscopy Conference MC2019, 1.-5. Sept 2019 in Berlin
- EMC2020 in Kopenhagen, 23.-28.8. 2020 (European conference)
- MC2021 in Vienna (D-A-CH conference)
- next Microbiol. conferences: VAAM 17.-20.03. 2019 Mainz




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7, 25 -- To: {frank_karl-at-ardl.com} , "microscopy server" {Microscopy-at-microscopy.com}
7, 25 -- Subject: Screen lifter blues
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From: benada-at-biomed.cas.cz
Date: Wed, 5 Dec 2018 14:14:07 -0600
Subject: [Microscopy] Re: [SPAM] Screen lifter blues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Frank,
It is really strange as Reinhard wrote. We have been running CM12 since
1985 up to 2014 when HT tank has gone. We had some problems with leaks
in viewing chamber (o-ring of diffraction beam stopper assembly and
sealing of 35 mm camera) during those years. But those leaks never led
to the microscope complete switch-off. Usually HT was switched off and
IGP pump too.
However, we observed that after strong discharge the CM12 was
sometimes switched off and this also restarted the EDAX system
connected to the CM12.

What tells you the plate exposure meter when you change the intensity
on the main screen? Is the exposure time changing?

I hope that you solve the problem soon.

Best regards and Merry Christmas

Oldrich

--
Oldrich Benada
Institute of Microbiology, Czech Acad. Sci.
Videnska 1083
142 20 Prague 4
Czech Republic

On Wed, 5 Dec 2018 10:05:28 -0600
frank_karl-at-ardl.com wrote:

} ----------------------------------------------------------------------------
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}
} Hello Everyone,
} Merry Christmas.
}
} My Philips CM12 TEM has a tendency to shut off if I lift the focus
} screen too fast (too hard?) to expose the bottom mount camera. This
} is relatively new to me, as I was using a side mount camera and never
} had to lift the plate.
}
} Is this normal?
}
} If it's not, other than being slow and delicate, what was the cure?
}
}
} Oh, yes, if you don't celebrate Christmas, that fine. I wish you the
} very best at this time of year.
}
}
}
}
} Stay safe...........
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}
}
} ==============================Original
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From: colijn.1-at-osu.edu
Date: Wed, 5 Dec 2018 19:42:21 -0600
Subject: [Microscopy] Screen lifter blues

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Frank,

It is a little unclear what you mean by the Microscope "shuts off". Is
it just the HT, the vacuum system, or all the electronics?

Assuming that it isn't all the electronics, it sounds like there is a
vacuum leak on the screen lift lever. If you are operating, the loss of
vacuum will shut off the emitter and the HT and (quite likely) the IGPs
as well. The vacuum page will then show the IGP shut off and V6 open so
that the Diff pump is pumping on the column and gun.

The fix would be to disassemble the screen lift feedthrough and replace
the O-rings.

Cheers,
Henk

----------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

------ Original Message ------
X-from: "frank_karl-at-ardl.com" {frank_karl-at-ardl.com}
To: "Colijn, Hendrik" {colijn.1-at-osu.edu}
Sent: 12/5/2018 11:09:55 AM

Hello Everyone,
Merry Christmas.

My Philips CM12 TEM has a tendency to shut off if I lift the focus
screen too fast (too hard?) to expose the bottom mount camera. This is
relatively new to me, as I was using a side mount camera and never had
to lift the plate.

Is this normal?

If it's not, other than being slow and delicate, what was the cure?


Oh, yes, if you don't celebrate Christmas, that fine. I wish you the
very best at this time of year.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305





----------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

----------------

Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu


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==============================End of - Headers==============================




From: sergei2-at-ornl.gov
Date: Thu, 6 Dec 2018 12:02:10 -0600
Subject: [Microscopy] Technical professional position open - SPM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

The Scanning Probe Microscopy group at ORNL is looking for technical
professional in the field of ultra high vacuum SPM. The description of
the position is available at:
https://jobs.ornl.gov/job/Oak-Ridge-Technical-Associate-Staff-Member-UHV-Scanning-Probe-Microscopy-TN-37830/521071900/

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Fri, 7 Dec 2018 06:45:33 -0600
Subject: [Microscopy] viaWWW:QMA 2019 Topical Conference

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Email: hlowers-at-usgs.gov Name: Heather Lowers

Organization: USGS

Title-Subject: [Filtered] QMA 2019 Topical Conference

Message: The Microanalysis Society is pleased to announce registration for QMA 2019, Quantitative
Microanalysis 2019, is now open. QMA 2019 will be held at the University of Minnesota, Minneapolis,
June 24-27, 2019. The conference will include user meetings, tutorials, technical platform and
poster presentations, vendor demonstrations, group discussions, and a conference banquet. Topics
covered include, but are not limited to, quantitative analysis by SEM/EDS and EPMA, compositional
mapping, sample preparation, microanalysis education, and reference materials.

Financial support for early career scholars, including undergraduate, graduate, post-doctoral, and
early career professional scientists who are within two years of their last degree is available
through reimbursement. A letter describing the details of this award are available on the website.
Visit the QMA 2019 website for updated information regarding registration, the technical program,
ECS financial support, travel to the venue, and accommodations.
https://the-mas.org/events/topical-conferences/qma-2019/

Please join us!
The QMA 2019 Organizing Committee


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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Dec 2018 14:21:47 -0600
Subject: [Microscopy] viaWWW:Materials Characterization Facility Job Opening

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


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Email: degraef-at-cmu.edu Name: Marc De Graef

Organization: Carnegie Mellon University

Title-Subject: [Filtered] Materials Characterization Facility Job Opening

Message: The Department of Materials Science and Engineering at Carnegie Mellon University in
Pittsburgh, PA, is looking for a Materials Characterization Facilities Specialist to work in the
Materials Characterization Facility. Interested parties should visit the following web site for
more details:

{https://cmu.taleo.net/careersection/2/jobdetail.ftl?job=2011151}

Regards,
Marc De Graef.


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From: leunissen-at-aurion.nl
Date: Wed, 12 Dec 2018 01:50:29 -0600
Subject: [Microscopy] In memoriam prof dr Hellmuth Sitte

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear members,

Prof Hellmuth Sitte passed away on Thursday 6th December in Innsbruck, aged 90.

Prof Sitte combined his professor and rectorship at the university of the Saarland in Homburg (Bio Medicine) with a deep interest and dedication for the development of instrumentation for electron microscopy. A pioneer in ultramicrotome design he had over 200 patents in the field of ultramicrotomy and cryo instrumentation to his name.

Many of you will have fond memories of him through the workshops that were conducted in Seefeld in Tirol where he introduced participants and guest staff not only to his passion for science and instrumentation, but also for his beloved mountains of the Northern Alps and the cultural wealth of the Innsbruck valley and South Tirol.

He and his family were amazing and welcoming hosts for many of us and the social evenings in his home were second to none.

These combined aspects have left a deep and unique impression.

Jan Leunissen
Dunedin, New Zealand

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7, 65 -- Subject: [Microscopy] In memoriam prof dr Hellmuth Sitte
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From: petereaton-at-hotmail.com
Date: Wed, 12 Dec 2018 06:07:45 -0600
Subject: [Microscopy] 2019 AFM Training Course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

The Porto AFM Workshop 2019 has been announced. This is the sixth
edition of the course.

The course will run from the 15th to 18th April. This is a training
workshop aimed at any researcher or scientist who wants to learn about
Atomic Force Microscopy, or increase their knowledge of the technique.
Following the successful courses that have run since 2011, the course
will include several hours hands-on training in acquiring images with
the AFM as well as AFM data processing. Lectures cover instruments,
sample preparation, AFM operation, data processing and applications.

For more information visit http://afmhelp.com/course, or email me at
afmhelp-at-gmail.com

Dr. Peter Eaton


==============================Original Headers==============================
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From: frank_karl-at-ardl.com
Date: Wed, 12 Dec 2018 09:45:37 -0600
Subject: [Microscopy] local TEM training

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Everyone,
Merry Christmas and Happy New Year.

I've always said I'm not really a TEM guy, but a PLM generalist with a side dish of SEM/EDS. ARDL is looking for a local TEM consultant to show us how to maximize alignment including filament changing.

While we do this when we have to, we use to depend heavy on our FEI service guy. Unfortunately, FEI no longer provides service for our CM12 and while our current contractor, TSS, does a wonderful job, they can't stop by and help tweek the scope when I fumble finger it. Just because I'm semi-retired doesn't mean I don't want to learn more..................

If you're a local to the Akron area, we would be interested in a little onsite training and assistance. Please contact me at:
Frank_Karl-at-ARDL.Com.






Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



==============================Original Headers==============================
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From: vray-at-partbeamsystech.com
Date: Wed, 12 Dec 2018 13:39:35 -0600
Subject: [Microscopy] Postdoc position, X-Ray/FIB correlative imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers:

I'd like to draw your attention to postdoc opening in the group I'm
affiliated with at UCONN:

http://academicjobsonline.org/ajo/UConn/TechPark/2019230

Recent graduates and about-to-graduate PhD students are encouraged to apply.

--
Valery Ray (also with REFINE/UCONN)
===================================
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
MEO Engineering / PBS&T
290 Broadway, Suite 298
Methuen, MA 01844, USA
www.partbeamsystech.com
www.freudlabs.com

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From: microscopy.listserver-at-gmail.com
Date: Thu, 13 Dec 2018 23:03:10 -0600
Subject: [Microscopy] viaWWW:CLEM Seminar at Max Planck Florida Institute -rescheduled

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Email: naomi.kamasawa-at-mpfi.org Name: Naomi Kamasawa

Organization: Max Planck Florida Institute for Neuroscience

Title-Subject: [Filtered] CLEM Seminar at Max Planck Florida Institute -rescheduled

Message: This is an announcement for our re-scheduled event.

The Electron Microscopy Facility of the Max Planck Florida Institute for Neuroscience (Jupiter, FL)
will host a MPFI-ZEISS labs-at-location partnership launch event on January 17th, 2019. The event
includes a seminar focusing on the latest topics of Correlative Light-Electron Microscopy (CLEM), a
demo for the new ZEISS Focal Charge Compensation module on the 3View-Gemini SEM300 system in the EM
facility, and a partnership signing ceremony followed by a reception.

The event is open to all levels of researchers who are interested in utilizing the latest advances
in electron microscopy.
For more details and registration, please check our website;
https://www.maxplanckflorida.org/events/other/mpfi-zeiss-labsatlocation-partnership-launch/

Registration is open until January 10th, 2019.

Please contact Naomi Kamasawa for more questions.
naomi.kamasawa-at-mpfi.org

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From: microscopy.listserver-at-gmail.com
Date: Thu, 13 Dec 2018 23:04:01 -0600
Subject: [Microscopy] viaWWW:Position Available: Senior Research Fellow (TEM) at UNSW,

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Email: r.webster-at-unsw.edu.au Name: Richard Webster

Organization: UNSW, Sydney

Title-Subject: [Filtered] Position Available: Senior Research Fellow (TEM) at UNSW, Sydney

Message: We have a job opening for a Senior Research Fellow take a leading role in developing
transmission electron microscopy, especially aberration-corrected transmission electron microscopy
in the Electron Microscope Unit at UNSW, Sydney. This position is a 5 year appointment with options
for renewal. All enquiries should be made to Prof Richard Tilley: r.tilley-at-unsw.edu.au.
More details at:
http://external-careers.jobs.unsw.edu.au/cw/en/job/495660/senior-research-fellow-transmission-electron-microscopy

Could you please forward this on to anyone you know that might be interested in the position?


Dr Richard F Webster

Research Associate, Electron Microscope Unit, Mark Wainwright Analytical Centre
Rm B65, Chemical Sciences Building

UNSW Sydney
NSW 2052 AUSTRALIA
T: +61 (0)2 9385 6709
M: +61 (0)431 534 332
E: r.webster-at-unsw.edu.au
W: unsw.edu.au

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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Dec 2018 07:06:37 -0600
Subject: [Microscopy] viaWWW:Pathfinder Spectral Imaging Question

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Email: tomw-at-uidaho.edu Name: Tom Williams

Organization: Univ of Idaho

Title-Subject: [Filtered] Spectral Imaging Question

Message: Greetings,

My lab switched to ThermoFisher Pathfinder software from an older NSS. Still on the "uphill" part
of the learning curve. My question concerns accessing the EDS map images in Spectral Imaging mode.
How can I access the individual map images in a format that can be read into ImageJ? Can I get a
TIFF image for example? Perhaps a silly question but right now I'm unable to solve this nut!

Thanks in advance.

Tom

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From: wesaia-at-iastate.edu
Date: Mon, 17 Dec 2018 10:12:18 -0600
Subject: [Microscopy] viaWWW:Pathfinder Spectral Imaging Question

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are you sure you want to? Why?

I manage a lab and have students inquire about that from our Oxford Aztec system now and then. I tried to persuade them that the vendors' software can do a lot. They may not want to reinvent the wheel.

If they insist, it is a fairly easy manner to export the Oxford maps into TIF format, although I think it a poor choice. (I don't know what PathFinder offers.) It does not retain the scaling of the data but contains 8-bit image values from 0-255 where the data has been stretched to fit. Many of my good maps do not have 100 counts per pixel. Most have far less. That pixel data was integrated over many channels, so you are giving up a lot of potential information going to one, rescaled number.

If you are still interested in processing the data on your own, I suggest you look into what others have already done, e.g., Lispix. It was developed at NIST for working with data cubes of spectral data.
https://www.nist.gov/services-resources/software/lispix

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

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Email: tomw-at-uidaho.edu Name: Tom Williams
Organization: Univ of Idaho
Title-Subject: [Filtered] Spectral Imaging Question
Message: Greetings,

My lab switched to ThermoFisher Pathfinder software from an older NSS. Still on the "uphill" part of the learning curve. My question concerns accessing the EDS map images in Spectral Imaging mode.
How can I access the individual map images in a format that can be read into ImageJ? Can I get a TIFF image for example? Perhaps a silly question but right now I'm unable to solve this nut!

Thanks in advance.

Tom


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From: nizets2-at-yahoo.com
Date: Tue, 18 Dec 2018 04:47:09 -0600
Subject: [Microscopy] Fluorescent beads surviving histological treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

i am doing a difficult search and googling leads me to directions I don't want.
I hope you can help me with this.
I want to use fluorescent microparticles as positive controls for an injection in skin samples.
The skin samples are injected and then they are fixed, dehydrated and embedded in paraffin (using histoclear instead of xylol) using classical histological methodology.
I want to be able to detect the fluorescence in paraffin sections without any further treatment.
I don't want to detect the beads by immune detection or any labeling method on the paraffin sections.
Optimally, the beads are 3-5µm in size and have a fluorescence spectrum similar to TRITC.
Alternatively, I am looking for metallic beads (like gold or silver) with a diameter of several micrometers (unusual), which I can probably detect in bright field.
Vendors are welcome to answer too.

Many thanks in advance!

Stephane

PS: a colored solution is no alternative, I want to detect particles


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From: lcgould-at-med.cornell.edu
Date: Tue, 18 Dec 2018 09:17:21 -0600
Subject: [Microscopy] Fluorescent beads surviving histological treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

HI Stephane-
What are your microparticles composed of? Make sure that they are not soluble in alcohols or histoclear before your process your tissues. If you have any doubts, I would suggest freezing the samples and cutting cryosections. Tissues are fixed, cryo-protected with sucrose and then frozen, so there are no solvents involved. I can send you and excellent protocol that gives very good histology (off list, since Nestor's filters will block attachments). Please let me know if you would like to try that.

Joyeux Noel et bonne annee.

Lee Cohen-Gould
Microscopy Core Facility
(212)746-6146

Weill Cornell Medicine
Weill Cornell Medical College
https://weillcornellmicroscopy.org/ {https://urldefense.proofpoint.com/v2/url?u=https-3A__weillcornellmicroscopy.org_&d=DwMF-g&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=WGy9rNsJ0aftr-RZGhYifG7rQyiuTbXtsTp_tkrR4sU&s=L5S_-lX8w7d6DZZKPNbh9XZShx1oj5iwQGpenS97LzE&e=}




On 12/18/18, 5:53 AM, "nizets2-at-yahoo.com" {nizets2-at-yahoo.com} wrote:




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Dear colleagues,

i am doing a difficult search and googling leads me to directions I don't want.
I hope you can help me with this.
I want to use fluorescent microparticles as positive controls for an injection in skin samples.
The skin samples are injected and then they are fixed, dehydrated and embedded in paraffin (using histoclear instead of xylol) using classical histological methodology.
I want to be able to detect the fluorescence in paraffin sections without any further treatment.
I don't want to detect the beads by immune detection or any labeling method on the paraffin sections.
Optimally, the beads are 3-5µm in size and have a fluorescence spectrum similar to TRITC.
Alternatively, I am looking for metallic beads (like gold or silver) with a diameter of several micrometers (unusual), which I can probably detect in bright field.
Vendors are welcome to answer too.

Many thanks in advance!

Stephane

PS: a colored solution is no alternative, I want to detect particles


==============================Original Headers==============================
6, 32 -- From nizets2-at-yahoo.com Tue Dec 18 04:47:08 2018
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From: leunissen-at-aurion.nl
Date: Tue, 18 Dec 2018 09:34:55 -0600
Subject: [Microscopy] Re: Fluorescent beads surviving histological treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Stephane,

You could use gold nanoparticles, for instance a BSA stabilized gold tracer, of any size and use silver enhancement on the section to enlarge their size for bright field observation. Very easy.
Feel free to contact me off list, I will be happy to help.

Jan Leunissen
Aurion - ImmunoGold Reagents



On 18/12/2018, at 23:47, nizets2-at-yahoo.com wrote:




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Dear colleagues,

i am doing a difficult search and googling leads me to directions I don't want.
I hope you can help me with this.
I want to use fluorescent microparticles as positive controls for an injection in skin samples.
The skin samples are injected and then they are fixed, dehydrated and embedded in paraffin (using histoclear instead of xylol) using classical histological methodology.
I want to be able to detect the fluorescence in paraffin sections without any further treatment.
I don't want to detect the beads by immune detection or any labeling method on the paraffin sections.
Optimally, the beads are 3-5µm in size and have a fluorescence spectrum similar to TRITC.
Alternatively, I am looking for metallic beads (like gold or silver) with a diameter of several micrometers (unusual), which I can probably detect in bright field.
Vendors are welcome to answer too.

Many thanks in advance!

Stephane

PS: a colored solution is no alternative, I want to detect particles


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19, 68 -- Subject: Re: [Microscopy] Fluorescent beads surviving histological treatment
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From: christian.feldhaus-at-tuebingen.mpg.de
Date: Thu, 20 Dec 2018 03:34:10 -0600
Subject: [Microscopy] Re: Fluorescent beads surviving histological treatment

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

have you considered using ceramic microbeads instead of the metallic
ones? I've never used them but they should come in the size range you
desire.

Best,

Christian


On 18.12.18 11:49, nizets2-at-yahoo.com wrote:
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}
} Dear colleagues,
}
} i am doing a difficult search and googling leads me to directions I
} don't want.
} I hope you can help me with this.
} I want to use fluorescent microparticles as positive controls for an
} injection in skin samples.
} The skin samples are injected and then they are fixed, dehydrated and
} embedded in paraffin (using histoclear instead of xylol) using
} classical histological methodology.
} I want to be able to detect the fluorescence in paraffin sections
} without any further treatment.
} I don't want to detect the beads by immune detection or any labeling
} method on the paraffin sections.
} Optimally, the beads are 3-5µm in size and have a fluorescence
} spectrum similar to TRITC.
} Alternatively, I am looking for metallic beads (like gold or silver)
} with a diameter of several micrometers (unusual), which I can probably
} detect in bright field.
} Vendors are welcome to answer too.
}
} Many thanks in advance!
}
} Stephane
}
} PS: a colored solution is no alternative, I want to detect particles
}
} ==============================Original
} Headers==============================
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} 10:52:19 +0000
} 6, 32 -- Date: Tue, 18 Dec 2018 10:52:17 +0000 (UTC)
} 6, 32 -- From: Stephane Nizet {nizets2-at-yahoo.com}
} 6, 32 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 6, 32 -- Message-ID: {1051087293.6020267.1545130337926-at-mail.yahoo.com}
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} Headers==============================

--
Christian Feldhaus, PhD
Light Microscopy Facility
MPI for Developmental Biology

Max-Planck-Ring 5 (Spemannstrasse 35)
72076 Tübingen
Germany

deliveries to:
Max-Planck-Ring 1
72076 Tübingen

Tel.: ++49 7071 601443


==============================Original Headers==============================
11, 26 -- From christian.feldhaus-at-tuebingen.mpg.de Thu Dec 20 03:34:10 2018
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11, 26 -- From: Christian Feldhaus {christian.feldhaus-at-tuebingen.mpg.de}
11, 26 -- Subject: Re: [Microscopy] Fluorescent beads surviving histological treatment
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From: microscopy.listserver-at-gmail.com
Date: Sat, 22 Dec 2018 20:07:47 -0600
Subject: [Microscopy] viaWWW: FEI Quanta 200 ESEM Owners/Users/Maintenance Specialists

Contents Retrieved from Microscopy Listserver Archives
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Email: rvancamp-at-kettering.edu Name: Rick

Organization: Kettering University

Title-Subject: [Filtered] FEI Quanta 200 ESEM Owners/Users/Maintenance Specialists

Message: Hello,

We own the W-filament version of this ESEM and I am attempting to identify owners that are
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establish a network for collaboration in keeping this instrument operational.

Please contact me if you can offer assistance.

Thank you,

Rick

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