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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Jan 2019 07:19:39 -0600
Subject: [Microscopy] Administrivia: Happy New Year-2019

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
a free service to the world wide microscopy community, sponsored
jointly by your Friendly Neighborhood SysOp and the Microscopy Society
of America.

During 2018, the ListServer delivered your messages to more than thousands of subscribers
around the world, with minimal hassels (that I know about). For those of you that are
statistics junkies this year we decreased in traffic abit. You generated } 35 Gbits of Email traffic
and } 1.1 Million Email messages were sent out this year by my tired and old little server. Down
abit from previous years, but still a steady flow.

As usual you don't want to know how much Junk Mail and spam has been filtered out
far more than you might expect. Apologies to those that have problems with
my filters.


The complete Microscopy ListServer Archives for 2018-1994 (~ are on-line at

http://www.microscopy.com.

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Nestor
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P.S. Sorry for sending this out a day late this year, I've been busy in the lab
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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Jan 2019 07:21:38 -0600
Subject: [Microscopy] viaWWW: SBF/SEM

Contents Retrieved from Microscopy Listserver Archives
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Email: jpare-at-emory.edu Name: Jeff Pare

Organization: Emory University\Yerkes National Primate Center

Title-Subject: [Filtered] SBF/SEM

Message: Good morning everybody. Happy New Year to all! I am a lab supervisor at Yerkes and we are
using a company to perform "serial face block scanning electron microscopy" of brain tissue. We are
interested in finding other companies who perform that type of work.
Do you know of groups or companies that do SBF/SEM? If so please contact me at jpare-at-emory.edu

Thanks

Jeff

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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Jan 2019 07:23:25 -0600
Subject: [Microscopy] viaWWW: I need a copy of a Proceedings article (1988)

Contents Retrieved from Microscopy Listserver Archives
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Email: radek.pelc-at-seh.oxon.org Name: Radek Pelc

Organization: Czech Academy of Sciences

Title-Subject: [Filtered]

I need a copy of a Proceedings article (1988)

Message: If you still have the 1988 Proceedings, may I ask you to do me a favour, please, and scan
the following abstract by Dr. Gordon W. Ellis who is no longer alive:

---------------------------------------------------------------

Ellis G.W. (1988) Scanned aperture light microscopy. Proceedings of 46th Annual Meeting of EMSA, pp.
48-49.

The EMSA proceedings are available via AbeBooks.com, but the year 1988 is missing.

Thank you very much and I look forward to hear from you.

Yours sincerely,

Radek Pelc

P.S. Happy New Year!

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From: Str8.Creative-at-gmail.com
Date: 7 Jan 2019 10:27:30 -0800
Subject: re: Google Anlaytics worldwide traffic

Contents Retrieved from Microscopy Listserver Archives
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Subscribe please


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From noreply-at-realsocialsignals.co Mon Jan 7 05:28:14 2019
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From Str8.Creative-at-gmail.com Mon Jan 7 12:21:15 2019
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From: NegativeSEOthatWorks-at-gmail.com
Date: 8 Jan 2019 13:41:49 -0800
Subject: re: How to remove a site from top 10 for important keywords

Contents Retrieved from Microscopy Listserver Archives
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Hi All,

We have multiple open positions in the NUANCE Center here at Northwestern University. Please see brief position descriptions and application info below. Feel free to reach out if you want more information and please forward to anyone who may be interested.

Regards,
Ben


1. SEM Facility Manager
The SEM Facility Manager is responsible for all aspects of the SEM/FIB Facility. This includes: equipment purchasing, upgrades and maintenance; supervision of Microscopy and Imaging Specialist; user training and technical support on all SEM instruments and related equipment; course development and teaching of laboratory components of SEM related courses; development of new analytical techniques and capabilities; providing analytical services for both internal NU and external industrial clients; conducting facility tours and demonstrations. In addition, this position has primary technical responsibility for the dual-beam Focused Ion Beam (FIB) instrument and assists in sample preparation for all electron microscopy, including Transmission Electron Microscopy (TEM).

MS/PhD, 4-5+ years hands-on characterization experience (post-degree) and 2+ years facility management/user training experience preferred.

Apply Here: https://careers.northwestern.edu/psp/hr92prod_er/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&SiteId=1&FOCUS=Employee&JobOpeningId=34899&PostingSeq=1

2. TEM Facility Manager
The TEM Facility Manager is responsible for training and assisting users on conventional and advanced Scanning-Transmission and Transmission Electron Microscopy (S/TEM), facilitating laboratory sessions for microscopy courses, and conducting analysis and characterization using advanced electron microscopes.

The successful candidate will develop an informed and well-trained user base in advanced microscopy and related sample preparation and characterization instruments. The incumbent will participate in user training, hands-on assistance, development of professional and short-courses, and overall facility development. Essential to the success of this position is enhancing both the visibility and advanced utilization of the S/TEM instruments to promote scholarly activities among the faculty, student and staff user base.

The selected candidate will regularly operate conventional and advanced S/TEM analysis, consult and collaborate with users on experiment design & research in electron microscopy, sample preparation and general lab equipment. There are ample opportunities for independent and collaborative research utilizing advanced S/TEM. With the recent installation of an advanced aberration-corrected S/TEM instrument, prior experience with AC-S/TEM is desirable, but not required.

Materials should be submitted as a single PDF to mailto:nuance-at-northwestern.edu. Complete applications will include: Introduction letter, Curriculum Vitae, Statement of plans for research (3 to 5 pages), Contact information for three references (names, postal & email addresses, phone number).

3. TEM Postdoctoral Research Associate
Under supervision of Dr. Xiaobing Hu, the selected candidate will develop advanced TEM applications and in-situ TEM methods on various materials system including engineering alloys, battery materials, thermoelectric materials and organic perovskite solar cells. The incumbent will oversee training of NUANCE users and collaborate with many excellent research groups in the department of Material Science and Engineering and Chemistry. As there will be ample opportunity to publish in top journals and assist in writing research and equipment proposals, the candidate must have excellent written and oral communication skills.

Research - Work closely with TEM manager to design and implement research methods in support of advanced materials research projects.
Training - Design and implement training curriculum for new, intermediate and advanced TEM users (students, postdocs, research staff).
Collaboration - Work with other faculty groups to design and implement TEM experiements specific to their research needs.

Applicants should send as a single PDF, 1. introduction letter (including research goals), 2. CV, 3. three references to Dr. Xiaobing Hu (mailto:xbhu-at-northwestern.edu).


-----------------------------------------------------------------------------------------------------------
Ben Myers, PhD

Director of Operations
SHyNE Resource, NUANCE Center

Northwestern University
2220 Campus Dr.
Evanston, IL 60208
(847) 467-1081
shyne.northwestern.edu





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From ronaldfaust205x-at-gmail.com Mon Jan 7 19:47:40 2019
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From NegativeSEOthatWorks-at-gmail.com Tue Jan 8 15:35:31 2019
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From: microscopy.listserver-at-gmail.com
Date: Tue, 8 Jan 2019 17:22:52 -0600
Subject: [Microscopy] viaWWW:Postdoctoral Position at ORNL on in situ microscopy/atomic

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-------- Forwarded Message --------


X-from: unocicrr-at-ornl.gov

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Email: unocicrr-at-ornl.gov Name: Ray Unocic

Organization: Oak Ridge National Laboratory

Title-Subject: [Filtered] Postdoctoral Position at ORNL on in situ microscopy/atomic manipulation

Message:
https://jobs.ornl.gov/job/Oak-Ridge-Postdoctoral-Research-Associate-In-situ-Microscopy-of-Nanoscale-Materials-TN-37830/528605400/

We are seeking a Postdoctoral Research Associate to support the development and application of
directed atomic manipulation of materials using aberration corrected scanning transmission electron
microscopy (STEM). The specific focus of your research will be to understand and control electron
beam interactions to understand factors that will guide the directed fabrication and atomic
manipulation of 1D, 2D, and 3D materials. You will contribute to the development of novel electron
beam scan control and feedback strategies, perform in situ microscopy experiments under controlled
environmental conditions and as a function of external stimuli, and you will work closely with Oak
Ridge National Laboratory (ORNL) scientists on materials characterization, synthesis, and theory to
experimentally validate predictive models. You will also collaborate with machine learning experts
at ORNL to provide experimental data input.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 8 Jan 2019 17:23:44 -0600
Subject: [Microscopy] viaWWW:SEM simulation programs

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Email: mcconkie.thomas-at-gmail.com Name: Thomas McConkie

Organization: The Design Knowledge Company

Title-Subject: [Filtered] SEM simulation programs.

Message: I am looking for either opensource or for purchase SEM simulation programs. I am not
looking for a virtual SEM for training but one that I can use to create theoretical images from
known substrate patterns of varying materials like integrated circuits. I am new to the simulation
SEM field and any information would be helpful.

Thank you.

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From: FBGroupsPosting-at-gmail.com
Date: 9 Jan 2019 04:35:40 -0800
Subject: re: Reach Millions of FB Groups Members within days

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Email: roger.ristau-at-uconn.edu Name: Roger Ristau

Organization: University of Connecticut

Title-Subject: [Filtered] TEM apertures

Message: While describing the operation of the TEM to students, I note that all the conventional
textbooks depict the objective aperture as below the objective lens, that is, they show the sequence
in ray diagrams as: specimen, lower objective lens, objective aperture coincident with the back
focal plane. The problem is that the objective aperture is phycically located above the lower
objective lens in our TEM: i.e. specimen, objective aperture, lower objective lens.
The thing I can't get my head around is how the back focal plane can be coincident with the
objective lens in the second case? Or is this a case of a virtual aperture?

Confused in Connecticut

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From: microscopy.listserver-at-gmail.com
Date: Wed, 9 Jan 2019 06:39:27 -0600
Subject: [Microscopy] viaWWW:Job Offer, University of Bayreuth, Bavarian Polymer Institute

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Email: info-at-bpi.de Name: Susanne Wolf

Organization: Bavarian Polymer Institute

Title-Subject: [Filtered] Job Offer, University of Bayreuth, Bavarian Polymer Institute

Message: Dear All,

In the KeyLab "Electron and Optical Microscopy" of the Bavarian Polymer Institute of the University
of Bayreuth, the following full-time position is to be filled as soon as possible, initially for a
limited period:

Research Associate
For the future Director of Electron Microscopy
pay grade: 13 TV-L (100%)

Application Deadline: 15. February 2019

If you are interested, you can find more information at:

https://www.uni-bayreuth.de/de/universitaet/arbeiten-an-der-universitaet/stellenangebote/wiss-personal/BCG-8/index.html

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From: Improve.DA.of.your.website-at-gmail.com
Date: 10 Jan 2019 03:01:09 -0800
Subject: Increase your website DA on next Moz update

Contents Retrieved from Microscopy Listserver Archives
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The OL is essentially an immersion lens; focusing occurs over an extended region between the top and bottom pole pieces that contains the specimen. The top pole piece contributes to forming parallel illumination on the specimen, and a crossover occurs above the lower pole piece, so an objective aperture can be situated below the specimen plane, but above the lower pole piece. A second crossover occurs below the OL, so our JEOL has apertures both within the lens and below the lens.

I wish the apertures were closer to the true back focal plane(s), though. They tend to limit the field of view at low magnifications.

-Phil
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Email: roger.ristau-at-uconn.edu Name: Roger Ristau

Organization: University of Connecticut

Title-Subject: [Filtered] TEM apertures

Message: While describing the operation of the TEM to students, I note that all the conventional textbooks depict the objective aperture as below the objective lens, that is, they show the sequence in ray diagrams as: specimen, lower objective lens, objective aperture coincident with the back focal plane. The problem is that the objective aperture is phycically located above the lower objective lens in our TEM: i.e. specimen, objective aperture, lower objective lens.
The thing I can't get my head around is how the back focal plane can be coincident with the objective lens in the second case? Or is this a case of a virtual aperture?

Confused in Connecticut

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From jeverett397fr-at-gmail.com Wed Jan 9 21:24:47 2019
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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Jan 2019 08:03:20 -0600
Subject: [Microscopy] viaWWW:Instrument Scientist (Electron Microscopy) position

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Email: Ursel.Bangert-at-ul.ie Name: Ursel Bangert

Organization: Bernal Institute, University of Limerick

Title-Subject: [Filtered] Instrument Scientist (Electron Microscopy) position

Message: We have a job vacancy for an Instrument Scientist in Electron Microscopy at UL (see below),
and we would appreciate very much if you could bring this to the attention of people (in your
institution or elsewhere) who might be interested, and generally spread the word.

Title: Instrument Scientist (Electron Microscopy)

Job Description: The Bernal Institute at UL requires an Instrument Scientist to support the research
activities of the Institute with focus on the area of transmission electron microscopy. The
Institute has a double aberration corrected, monochromated FEI Titan Cubed Themis TEM with
analytical facilities (SuperEDX and QuantumEELS) and sample holders for in-situ TEM measurements
under heating, biasing, in liquids and gases as well as under cryo-conditions. This Titan has
furthermore an ultra-fast and-sensitive (K2-IS) camera, for ultra-high resolution as well as in-situ
imaging and spectroscopic analysis of materials.
Requirements:
PhD in materials science/electron microscopy/chemistry/physics/ engineering or a related discipline
OR equivalent experience in a research environment (equivalent 4 years fulltime research after
primary degree). The full description and information for the position can be found at the
following link : http://www.ul.ie/hrvacancies/ . For additional information contact Prof Ursel
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From: John.Mardinly-at-asu.edu
Date: Thu, 10 Jan 2019 14:37:17 -0600
Subject: [Microscopy] Re: [EXT] viaWWW:TEM apertures

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

The second objective aperture on JEOL microscopes with an ultra high resolution pole-piece is actually not below the lens, but is introduced through a
hole drilled in the lower pole piece, substantially below the gap.

A. John Mardinly, Ph.D., P.E.


} On Jan 9, 2019, at 4:45 PM, Phil.Ahrenkiel-at-sdsmt.edu wrote:
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} The OL is essentially an immersion lens; focusing occurs over an extended region between the top and bottom pole pieces that contains the specimen. The top pole piece contributes to forming parallel illumination on the specimen, and a crossover occurs above the lower pole piece, so an objective aperture can be situated below the specimen plane, but above the lower pole piece. A second crossover occurs below the OL, so our JEOL has apertures both within the lens and below the lens.
}
} I wish the apertures were closer to the true back focal plane(s), though. They tend to limit the field of view at low magnifications.
}
} -Phil
} -----Original Message-----
} X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
} Sent: Tuesday, January 8, 2019 4:29 PM
} To: Ahrenkiel, Phil {Phil.Ahrenkiel-at-sdsmt.edu}
} Subject: [EXT] [Microscopy] viaWWW:TEM apertures
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} X-from: roger.ristau-at-uconn.edu
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} Email: roger.ristau-at-uconn.edu Name: Roger Ristau
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} Organization: University of Connecticut
}
} Title-Subject: [Filtered] TEM apertures
}
} Message: While describing the operation of the TEM to students, I note that all the conventional textbooks depict the objective aperture as below the objective lens, that is, they show the sequence in ray diagrams as: specimen, lower objective lens, objective aperture coincident with the back focal plane. The problem is that the objective aperture is phycically located above the lower objective lens in our TEM: i.e. specimen, objective aperture, lower objective lens.
} The thing I can't get my head around is how the back focal plane can be coincident with the objective lens in the second case? Or is this a case of a virtual aperture?
}
} Confused in Connecticut
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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Jan 2019 07:38:02 -0600
Subject: [Microscopy] viaWWW:TEM sample for Outreach

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


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Email: mike.toalson-at-elementpi.com Name: Mike Toalson

Organization: element Pi, LLC

Title-Subject: [Filtered] TEM sample for Outreach

Message: Would anyone be willing to donate a few prepared TEM samples like interesting tissue or
bacteria or other cellular specimens ready for analysis? These would be used for educational
outreach at the high school and undergrad level. They would need to work with STEM mode in a 30kV
max SEM so tissue sections would need to be fairly thin.

Or does anyone know where you can buy such sample standards? None of the EM consumable suppliers
seem to carry anything like this.

Any questions or to provide a few samples, please contact me.

Much Appreciated!!

Mike Toalson
element Pi, LLC
833-314-1593

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From: stefan.diller-at-t-online.de
Date: Sat, 12 Jan 2019 05:47:06 -0600
Subject: [Microscopy] ZEISS EM109 manuals needed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

does anyone have the manuals "A", "B" and "C" (user-manuals and alignment procedures) - preferably in German language or even
electronic schematics of the ZEISS EM 109 transmission electron microscope available in PDF or can copy the manuals ? I would
happily compensate for the costs...


Best wishes,

Stefan


--


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Arndtstrasse 22
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Websites:
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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11, 23 -- From: Stefan Diller {stefan.diller-at-t-online.de}
11, 23 -- Subject: ZEISS EM109 manuals needed
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From: microscopy.listserver-at-gmail.com
Date: Tue, 15 Jan 2019 20:06:40 -0600
Subject: [Microscopy] viaWWW: QMA 2019 abstract submission open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




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Email: mastopicalconference-at-gmail.com Name: Heather Lowers

Organization: MAS

Title-Subject: [Filtered] QMA 2019 abstract submission open

Message: Hello

Abstract submission for Quantitative Microanalysis 2019 is open! QMA 2019 is an MAS Topical
Conference focused on topics related to quantitative microanalysis by wavelength-dispersive (WDS)
and energy-dispersive (EDS) spectrometry.

The conference will be held June 24-27, 2019 at the University of Minnesota, Minneapolis.
For a list of programmatic topics, registration, abstract template, and submission please visit our
website:
https://the-mas.org/events/topical-conferences/qma-2019/

We look forward to your contribution,
QMA 2019 Organizing Committee

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From: microscopy.listserver-at-gmail.com
Date: Wed, 16 Jan 2019 07:32:26 -0600
Subject: [Microscopy] Fwd: needs a new Stereomicroscope.................

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Frank Karl {frank_karl-at-ardl.com}

We need a new stereomicroscope and I'm not current on what's available. Anyone with a recommendation?

I'd like:
Apochromatic lenses,
Trans illuminated base,
Trinocular head and camera, something with good color and resolution,
Computer software with easy file tracking, image processing, measurements and annotation,
Magnification range from about 1 to100-ish X (with high NA objectives, of course).

We'd like if possible,
fine focus as well as coarse,
flat field,
good mechanical stability.

I feel a little like that commercial where the neighbor ask his buddy to do all the leg work to hire
a contractor. Sorry.

I'm very much open to responses from dealers.

Thanks in advance!




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Jan 2019 07:25:04 -0600
Subject: [Microscopy] Fwd: Re: Fwd: needs a new

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Frank,

Check out Mager Scientific - Nikon http://www.magersci.com/nikonmicroscopes/
and Nusbaum - Leica https://nuhsbaum.com/nuhsbaum-products/

They're the dealers for Nikon & Leica your (our) area, and both have what you're looking for. There
are others, like Olympus https://www.olympus-lifescience.com/en/
and Zeiss, distributed in the Midwest by Lukas lukasmicroscope.com

Plus industrial inspection stereoscopes, but I don't have URLs handy for those.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office
989 774-7567 lab

-----Original Message-----
X-from: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}
Reply-To: "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com}



X-from: Basgall,Edward {ejb63-at-drexel.edu}


I always check to see what BidService has in pre-owned stereo scopes.


Get Outlook for iOS {https://aka.ms/o0ukef}
----------------------------------------------------------------------------------------------------
*From:* microscopy.listserver-at-gmail.com
*Sent:* Wednesday, January 16, 2019 08:41
*To:* Basgall,Edward
*Subject:* [Microscopy] Fwd: needs a new Stereomicroscope.................



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-------- Forwarded Message --------


X-from: Frank Karl {frank_karl-at-ardl.com}

We need a new stereomicroscope and I'm not current on what's available. Anyone with a recommendation?

I'd like:
Apochromatic lenses,
Trans illuminated base,
Trinocular head and camera, something with good color and resolution,
Computer software with easy file tracking, image processing, measurements and annotation,
Magnification range from about 1 to100-ish X (with high NA objectives, of course).

We'd like if possible,
fine focus as well as coarse,
flat field,
good mechanical stability.

I feel a little like that commercial where the neighbor ask his buddy to do all the leg work to hire
a contractor. Sorry.

I'm very much open to responses from dealers.

Thanks in advance!




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Jan 2019 07:25:46 -0600
Subject: [Microscopy] Fwd: Re: Fwd: needs a new

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




X-from: Sathya Srinivasan {scitecheditor-at-gmail.com}



Hi Frank,
If you want a branded one, get a quote from your local microscope sales rep (Leica, Olympus, Nikon
or Carl Zeiss or other good companies which I would have missed out). You can google the company
names and find the local reps contact in your area. There are other microscope dealers (like
http://precisionmicroscopesales.com/index.php?route=product/category&path=59_63).  With a few
modifications and attachments, you can convert your bright field stereo microscope into a
fluorescence microscope (eg. http://www.nightsea.com/. If you are not bothered too much on image
resolution). Depends on what you are looking for. There is also the Echo Revolve
(https://discover-echo.com/revolve) where you can use them as upright and inverted with bright field
and fluorescence capability. You can also check out the adapters for microscope where you can
convert a simple microscope into digital using your cell phone camera -
https://novagrade.com/shop/phone-adapter-microscope-edition/ (Resolution is the criteria again). The
range and price is so wide, it should be decided by what you want the microscope for. Ask the
vendors to bring in and show how it can be applied for your use (You will be surprised by some of
the features including the software - make sure if the images generated using the microscope
software can be opened and annotated using other software and the cost for the full version - if a
free version is available, support, etc.). No commercial interest and the order of the companies is
nothing to do with the quality or my preference - I love all the microscopes from any vendor.

Good luck

Sathya Srinivasan
Director
Imaging and Morphology Support Core
ONPRC
Beaverton, OR 97006

On Wed, Jan 16, 2019 at 5:42 AM {microscopy.listserver-at-gmail.com
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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }

We need a new stereomicroscope and I'm not current on what's available.  Anyone with a
recommendation?

I'd like:
Apochromatic lenses,
Trans illuminated base,
Trinocular head and camera, something with good color and resolution,
Computer software with easy file tracking, image processing, measurements and annotation,
Magnification range from about 1 to100-ish X (with high NA objectives, of course).

We'd like if possible,
fine focus as well as coarse,
flat field,
good mechanical stability.

I feel a little like that commercial where the neighbor ask his buddy to do all the leg work to
hire
a contractor.  Sorry.

I'm very much open to responses from dealers.

Thanks in advance!




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio  44305


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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Jan 2019 08:11:38 -0600
Subject: [Microscopy] viaWWW: Hitachi S-570 SEM: Fixed Objective Holder Dimensions

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Email: mbrazil-at-vergason.com Name: Michael Brazil

Organization: Vergason Technology, Inc.

Title-Subject: [Filtered] Hitachi S-570 SEM: Fixed Objective Holder Dimensions

Message: I have recently acquired a Hitachi S-570 SEM (1984 vintage). Scope works well, however it
arrived missing the holder for the fixed objective aperture. Hitachi service does not offer these
parts. If anyone is parting out a similar model, I'm a customer. If anyone has a such a scope, I
would be grateful for careful measurements that would allow a machine shop to duplicate the holder.

Thanks,

Michael Brazil
Senior Scientist
Vergason Technology
mbrazil-at-vergason.com

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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Jan 2019 08:12:37 -0600
Subject: [Microscopy] viaWWW: M&M 2019 Symposium P10: Microscopy and microanalysis

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Email: donggaozhao-at-gmail.com Name: Donggao Zhao

Organization: University of Missouri-Kansas City

Title-Subject: [Filtered] Paper submission for M&M 2019 Symposium P10: Microscopy and microanalysis
techniques in characterizing natural and synthetic materials

Message: Dear colleagues,
We would like to draw your attention to the following M&M 2019 session, August 4-8, 2019, Portland,
Oregon: The submission portal is open! Submit your 2-page paper as soon as possible at
https://www.microscopy.org/MandM/2019/. The deadline is Friday, February 15.
M&M 2019 Symposium P10: Applications of integrated electron probe microscopy and microanalysis
techniques in characterizing natural and synthetic materials

Description: Electron microbeam techniques, such as SEM/ESEM, EPMA and TEM/STEM, use a focused
electron probe or a small parallel electron beam to bombard a specimen and generate signals at a
scale from micrometer down to Angstrom level. These signals include secondary electron (SE),
backscattered electron (BSE), characteristic X-ray, cathodoluminescence (CL), transmitted electron,
diffracted or scattered electron, etc. Information acquired using these signals includes image,
chemistry and crystal structure of a specimen at micrometer, nanometer and sub-Angstrom levels.
We welcome contributions covering the entire range of electron probe microscopy and microanalysis of
natural and synthetic materials, such as: Imaging from SE, BSE, X-ray, CL, charge contrast,
transmitted electron, diffracted or scattered electron, etc. Qualitative and quantitative
determination of chemistry of natural and synthetic materials Repeatability, reproducibility and
compatibility of quantitative microanalysis standards Crystal structure determination using
electron diffraction or electron backscattered diffraction (EBSD)

Feel free to share this information to other potentially interested colleagues. We are very much
looking forward to seeing you in Portland, Oregon!
Best Regards,

Organizers:

Donggao Zhao Email: zhaodo-at-umkc.edu
Affiliation: University of Missouri-Kansas City
Phone 1: 816-235-2072 (W)
Phone 2: 803-732-6280 (M)

Minghua Ren
Email: minghua.ren-at-unlv.edu
Affiliation: University of Nevada, Las Vegas Phone 1: 702-774-1465 (W)
Phone 2: 915-217-4392 (M)

Owen Neill Email: okneill-at-umich.edu Affiliation: University of Michigan Phone 1: 734-615-6657 (W)
Phone 2: (207) 653-6331 (M)


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From: microscopy.listserver-at-gmail.com
Date: Fri, 18 Jan 2019 05:10:13 -0600
Subject: [Microscopy] viaWWW:EDS mapping on bacteria cell embedded in epoxy resin

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Email: fei.long-at-queensu.ca Name: Fei Long

Organization: Queen's University

Title-Subject: [Filtered] EDS mapping on bacteria cell embedded in epoxy resin

Message: I am trying to do EDS elemental analysis on microtome sesctioned bacteria cells embedded in
epoxy resion with TEM at 200kV in STEM mode. However, the electron beam burn the sample instantly.
Does anyone have good experience in doing chemical analysis on biology samples with TEM by EDX? Is
there any irradiation resistant epoxy that I can use for the bacteria embedding? Lastly, if I cool
the sample to low temperatures with liquid nitrogen by using a cooling holder in TEM, does it help
to reduce the beam burning?

Any suggestions will be much appreciated!

Best,

Fei

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From: microscopy.listserver-at-gmail.com
Date: Fri, 18 Jan 2019 05:57:31 -0600
Subject: [Microscopy] Call for proposals for HaraldRoseLecture 2019

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Stephen Perry {steve.sem-at-icloud.com}





-------- Forwarded Message --------


X-from: Gemming, Dr. Thomas {T.Gemming-at-ifw-dresden.de}

Dear Representatives of the (Electron) Microscopy Societies and otherwise involved microscopists,

please find attached the announcement of the Harald Rose Distinguished Lecture 2019 award of the
German Society for Electron Microscopy. Please distribute the announcement within your society and
interested colleagues. The application deadline is February 28th. The distinguished lecture will
take place at the microscopy conference MC2019 in Berlin in the first week of September 2019.

The announcement can also be found as pdf at:

http://www.dge-homepage.de/hrl2019_announcement.pdf


Best regards and thank you for your help in spreading this announcement,

Thomas Gemming
Executive secretary of the German Society for Electron Microscopy



Dr. Thomas Gemming
Act. Director of Institute for Complex Materials Leibniz-Institut fr Festkrper- und
Werkstoffforschung Dresden e.V.
Helmholtzstr. 20, 01069 Dresden

Tel.: +49 (351) 4659 - 298 , Fax: -9298
E-Mail: t.gemming-at-ifw-dresden.de

Executive Secretary of German Society for Electron Microscopy http://www.dge-homepage.de/



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From: microscopy.listserver-at-gmail.com
Date: Tue, 22 Jan 2019 19:05:50 -0600
Subject: [Microscopy] viaWWW:Installing GMS3 on Mac?

Contents Retrieved from Microscopy Listserver Archives
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Hello Fei,

In my experience, most of the epoxy resins designed for
(ultra)microtomy are reasonably durable under electron beams, even up
to 300kV. You didn't mention whether you are using a FEG or thermionic
microscope, so I'm not exactly sure what mechanism might be causing
the damage you are experiencing. With thermionic microscopes, you
probably don't have enough current density in STEM mode to literally
drill through your sample and most of the damage you are seeing is
likely from charging. Coating your sample with a few angstroms or a
nanometer of carbon can completely eliminate the charging and
stabilize the sample. Sometimes you need to coat both sides with a
particularly ornery sample.

For a FEG, you can easily have enough current density in STEM mode to
sputter through your sample. You'll need to adjust your condenser
illumination conditions and condenser aperture to reduce current
density. If you're using reasonable settings that have worked well
with previous similar samples, then the problem is probably charging.
A small coating of carbon, as mentioned above, can eliminate that
issue.

It's still possible that the epoxy used is not one recommended for
electron microscopy. I'll let the more experienced microtomy and
biological TEM people address those concerns.

Good luck,
Chris


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} Title-Subject: [Filtered] EDS mapping on bacteria cell embedded in epoxy resin
}
} Message: I am trying to do EDS elemental analysis on microtome sesctioned bacteria cells embedded in
} epoxy resion with TEM at 200kV in STEM mode. However, the electron beam burn the sample instantly.
} Does anyone have good experience in doing chemical analysis on biology samples with TEM by EDX? Is
} there any irradiation resistant epoxy that I can use for the bacteria embedding? Lastly, if I cool
} the sample to low temperatures with liquid nitrogen by using a cooling holder in TEM, does it help
} to reduce the beam burning?
}
} Any suggestions will be much appreciated!
}
} Best,
}
} Fei
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7, 42 -- Subject: Re: [Microscopy] viaWWW:EDS mapping on bacteria cell embedded in
7, 42 -- epoxy resin
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From ralpzand8wo-at-gmail.com Fri Jan 18 10:47:23 2019
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Fei;
There are several ways to reduce damage, but they come with reduced spatial resolution: 1) Reduce beam current (Spot size) and/or slightly defocus the beam. You will have less signal, requiring longer acquisition time and drift will be a factor. 2) Use a thicker section. Due to beam spreading, resolution may be reduced. 3) Cooling. Cold stages have inherently more drift. 4) Sputter a carbon or aluminum coating on the EXIT surface of the specimen. That will reduce charging and conduct heat away from the beam spot but will add some background to the spectra. 5) Some combination of the previous 4.

A. John Mardinly, Ph.D., P.E.


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} Title-Subject: [Filtered] EDS mapping on bacteria cell embedded in epoxy resin
}
} Message: I am trying to do EDS elemental analysis on microtome sesctioned bacteria cells embedded in
} epoxy resion with TEM at 200kV in STEM mode. However, the electron beam burn the sample instantly.
} Does anyone have good experience in doing chemical analysis on biology samples with TEM by EDX? Is
} there any irradiation resistant epoxy that I can use for the bacteria embedding? Lastly, if I cool
} the sample to low temperatures with liquid nitrogen by using a cooling holder in TEM, does it help
} to reduce the beam burning?
}
} Any suggestions will be much appreciated!
}
} Best,
}
} Fei
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Email: jennifer.cookman-at-ul.ie Name: Jennifer

Organization: University of Limerick

Title-Subject: [Filtered] Installing GMS3 on Mac?

Message: Dear All,

I want to ask the community if anyone has tried to installed GMS3 on a Mac?

I have tried to use wine and brew to install the right components but GMS3 seems to be incompatible
with Windows XP which is what wine is simulating.

Any help on this would be great! I do need the in situ portion of GMS3!

Best,

Jenn

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From: microscopy.listserver-at-gmail.com
Date: Tue, 22 Jan 2019 19:06:51 -0600
Subject: [Microscopy] viaWWW:FROM ATOMIC- TO MICRO-SCALE PROCESSES: call for abstracts

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




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Email: elena.belluso-at-unito.it Name: Elena Belluso

Organization: I-University of Torino -Dept of Earth Sciences and ​"G. Scansetti"
Interdepartmental Center for Studies on Asbestos and other Toxic Particulates,

Title-Subject: [Filtered] FROM ATOMIC- TO MICRO-SCALE PROCESSES: call for abstracts

Message: ​Dear Colleague,
We are pleased to invite you to submit an abstract for an oral presentation (or a poster) to the
session 4g MINERAL TRANSFORMATIONS IN MEDICAL AND ENVIRONMENTAL MINERALOGY: FROM ATOMIC- TO
MICRO-SCALE PROCESSES at Goldschmidt 2019, the international conference on geochemistry and related
subjects, scheduled from 18 to 23 August 2019 in Barcelona (E).
The key-note speaker of the session is Mihly Psfai (University of Pannonia).

The purpose of this session is to spread the more recent investigations dealing with the
relationship between minerals, environment, and human body.

This topic includes the study of naturally occurring minerals, their transformation in the
environment and within the human body, and minerals synthesized by living organism themselves.

Thus, the session covers a broad interdisciplinary field positioned between geology, mineralogy,
geochemistry, material science, and medicine.

Particular attention will be dedicated (but not limited) to contributions investigating the
synthesis, transformation and function of minerals in the environment, in living organisms, and
those minerals with an active role in human disease development.

This session welcomes contributions related to the study and the comprehension of the complex
relationships occurring between natural or anthropogenic environments and different minerals.

Among various targets, this session aims to present the state-of-the-art in this multifaceted and
complex topic and to develop interdisciplinary collaborations.

Note that the abstract deadline is March 29th (23:59 CET)
(https://goldschmidt.info/2019/abstracts/instructions#who)
We look forward to seeing you in Barcelona in August!
With ours best wishes for the New Year,
the convenors Elena Belluso (elena.belluso-at-unito.it) and Ruggero Vigliaturo (ruggero-at-sas.upenn.edu)
PS
The Goldschmidt Grant Program provides waived registration fees and travel support for early career
delegates residents of specific countries and for US students. See at
https://goldschmidt.info/2019/grants for details.






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From: microwink-at-gmail.com
Date: Wed, 23 Jan 2019 13:21:54 -0600
Subject: [Microscopy] TEM xsect sample preparation - repeated glue failure

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

I'm working with a student who has metal samples with a ~1um thick
layer of amorphous silicon oxynitride on the surface, and they want to
prepare cross section TEM samples. We have tried gluing the sample
sandwich together with Epo-Tek 353ND (also known as Gatan G1) and
MBond 610, and both stacks fell apart on cutting.

I assumed that the SiON layers were delaminating, but a visual
inspection using the optical microscope convinced me this was not
likely the underlying cause of the problem. Both epoxies used are not
expired and were cured according to the manufacturer's cure schedule.
I have had no issues with cross sections of other sample systems and
these exact same epoxies, so I wonder if we need to use a different
epoxy chemistry than these standards. Does anyone have experience with
the SiON sample system? Is there a better epoxy to use, like Araldite?
We would like to try to create a sample stack before giving up and
switching to attempting tripod/wedge polishing.

Thanks for any advice,
Chris

==============================Original Headers==============================
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From: duncan.alexander-at-epfl.ch
Date: Wed, 23 Jan 2019 13:54:39 -0600
Subject: [Microscopy] New online course on TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

We have launched a new online course on Transmission Electron Microscopy for Materials Science, by EPFL on the Coursera platform:

https://www.coursera.org/learn/microscopy

In this course, we provide a comprehensive introduction to TEM theory, including:
- the instrument and its optics
- the basics of electron diffraction and dynamical scattering
- the theory of phase contrast imaging.

Built from the ground up, the course is intended to be a starting point for understanding and working with TEM; to be used either as a stand alone resource, or in complement to books and in person teaching or coaching. We hope that it proves useful to our electron microscopy community.

Course registration is open to all and can be made at any time. For any questions about the course either PM me directly or use the course forums.

With regards
Duncan Alexander

EPFL-SB-IPHYS-LSME

==============================Original Headers==============================
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From: MuirD1-at-cardiff.ac.uk
Date: Fri, 25 Jan 2019 04:54:49 -0600
Subject: [Microscopy] MMC session on Quantitative Microscopy in Earth, Archaeological and

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Dear all,

Please consider submitting an abstract for our session on Quantitative Microscopy in Earth, Archaeological and Planetary Sciences at the Microscience Microscopy Congress (mmc2019) on July 4th 2019 in Manchester, UK. The deadline for submission is February 15th and we welcome contributions from as wide a field as possible in these disciplines.


Session description
*******************************************************************
Quantitative microscopic investigation is critical for advancing our understanding of scientific problems today. Researchers have access to a broad range of analytical equipment including, but not limited to, light, electron and gas ion microscopy, secondary ion mass spectrometry, 3-D correlative microscopy, X-ray tomography and associated spectroscopic techniques. We invite presentations on examples from Earth, Planetary and Archaeological Sciences where novel microscopy techniques have beenused to help construct quantitative datasets preferably with practical outcomes and applications which advance scientific and/or technical understanding.

https://www.mmc-series.org.uk/

Best wishes,

Duncan Muir
Cardiff University



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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 Jan 2019 06:53:42 -0600
Subject: [Microscopy] viaWWW: Aberrations: Electron Optics

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Email: gcanzalo-at-mtu.edu Name: Jerry Anzalone

Organization: Michigan Tech

Title-Subject: [Filtered] Aberrations: Electron Optics

Message: Good day, Microscopists:

I'm developing an online SEM demonstrator/simulator as an instructional aid and have come to realize
I know next to nothing about electron optics. Goldstein, et al., and others, make the categorical
claim that minimizing the working distance yields a probe least affected by lens aberrations, yet
the equation for the diameter of the spherical aberration disk of least confusion indicates the
diameter increases as the cube of convergence angle. What am I missing?

While I understand the interaction volume is order(s) of magnitude larger than the beam, largely
obviating the utility of calculating the effects of aberrations on probe diameter, I nonetheless
wish to produce an accurate simulation, at least within an order of magnitude. Any assistance,
including papers (that don't include triple integrals) is much appreciated. Ikea-like drawings might
be best.

Cheers and thanks,

Jerry

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From: a.bullen-at-ucl.ac.uk
Date: Fri, 25 Jan 2019 08:08:26 -0600
Subject: [Microscopy] 89th IUVSTA Workshop on Biological and Soft Matter Sample Preparation

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Dear all,

We are pleased to announce that registration is now open for the 89th IUVSTA workshop:

Biological and Soft Matter Sample Preparation for High Resolution Imaging by High Vacuum Techniques

19th-24th May 2019 in Zakopane, Poland.

Website:http://www.npl.co.uk/89th-iuvsta-workshop/

Topics Include:
Electron Microscopy, Cryo-SIMS and SIMS Imaging, NanoSIMS, Atom Probe Tomography and Cryo-Spectroscopies

Chairs: Dr Paula Rakowska (National Physical Laboratory, UK) and Dr Kirsty MacLellan-Gibson (National Institute for Biological Standards and Control, UK).


Best Wishes,

Dr Anwen Bullen (on behalf of Dr MacLellan-Gibson)


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From: microscopy.listserver-at-gmail.com
Date: Tue, 29 Jan 2019 06:39:57 -0600
Subject: [Microscopy] viaWWW: GWNIC CLEM Workshop June 10-14, 2019

Contents Retrieved from Microscopy Listserver Archives
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Hello all,

I have summarized the replies I received regarding this issue below:

1. "Since the SiON is ~1umthick, top monolayers probably do not
matter. I can suggest to try the following : 1. Plasma clean the
sample just before applying epoxy. We are using 4-5 min of pure Ar at
50W forward RF with range 5W in our GATAN Solarus plasma cleaner.
or 2. Sputter ~1-2 nm of Cr or Fe on the top surface before applying
epoxy. We use GATAN PECS to do that. Sputtering with any reactive
metal available in your lab, if Fe or Cr targets are not available,
should also work."

2. Many are concerned the film is delaminating from the metal
substrate. We should probably check this using the SEM, but we don't
see any evidence of delamination in the optical microscope (when
comparing failed glue specimen with pristine specimen).

3. Many suggested FIB. The student prefers a conventional xtem sample
preparation to generate a larger electron transparent region than the
FIB would provide, but if all else fails then we can fall back to the
FIB.

4. "A simple suggestion that, perhaps, might work (this is how we work):
Why don’t you inverse the order of operations? I mean: first, cut the
small pieces and then, glue them as a sandwich. Hopefully, the glued
pieces will hold together during the grinding steps. Good luck with the
ion milling, afterwards."

5. "I have used Devcon 5-minute epoxy for many years on all sorts of
samples with success. It is much more viscous than the other usual TEM
epoxies but, you know, the sample is ready for the next step in 5
minutes..."

Thanks for all of your help!

Cheers,
Chris

On Wed, Jan 23, 2019 at 2:30 PM {microwink-at-gmail.com} wrote:
}
}
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} Hello all,
}
} I'm working with a student who has metal samples with a ~1um thick
} layer of amorphous silicon oxynitride on the surface, and they want to
} prepare cross section TEM samples. We have tried gluing the sample
} sandwich together with Epo-Tek 353ND (also known as Gatan G1) and
} MBond 610, and both stacks fell apart on cutting.
}
} I assumed that the SiON layers were delaminating, but a visual
} inspection using the optical microscope convinced me this was not
} likely the underlying cause of the problem. Both epoxies used are not
} expired and were cured according to the manufacturer's cure schedule.
} I have had no issues with cross sections of other sample systems and
} these exact same epoxies, so I wonder if we need to use a different
} epoxy chemistry than these standards. Does anyone have experience with
} the SiON sample system? Is there a better epoxy to use, like Araldite?
} We would like to try to create a sample stack before giving up and
} switching to attempting tripod/wedge polishing.
}
} Thanks for any advice,
} Chris
}
} ==============================Original Headers==============================
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} 4, 39 -- From: Christopher Winkler {microwink-at-gmail.com}
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} 4, 39 -- Message-ID: {CAA8T2PPoF2psxockj2iiLsaYMJOQA6OjG9raJ3kdHDQFPeCSHA-at-mail.gmail.com}
} 4, 39 -- Subject: TEM xsect sample preparation - repeated glue failure
} 4, 39 -- To: Microscopy-at-microscopy.com
} 4, 39 -- Cc: Kaustubh Bawane {kauskb7-at-vt.edu}
} 4, 39 -- Content-Type: text/plain; charset="UTF-8"
} ==============================End of - Headers==============================


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From harremme142vue-at-gmail.com Fri Jan 25 18:00:28 2019
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Dear All, may I ask if anyone has any experience is using some 3rd Party makeshift controller to duplicate some functionality of the TEM control panel such as brightness and focus knob? And probably its for JEOL TEM with TEMCenter software.

I come across https://www.3dconnexion.eu/ for CAD software but wonder if one could tweak it for TEM control application.

Any pieces of advice are greatly appreciated!

Cheers,
Yee Yan, Tay
FACTS, NTU

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4, 32 -- TEM Control Panel?
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From: microscopy.listserver-at-gmail.com
Date: Wed, 30 Jan 2019 19:37:48 -0600
Subject: [Microscopy] Imaging Research Specialist Position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Louis Kerr {lkerr-at-mbl.edu}




I am pleased to announce a new staff position at the Marine Biological Laboratory.Those interested
in the position should submit an application, including a CV, a cover letter, and the names and
contact information for three references to mbl.edu/RESEA01039

We will begin reviewing applications on February 11, 2019.

**

*Imaging Research Specialist/Senior Research Specialist*

*MBL Microscopy and Imaging Facility*

The Marine Biological Laboratory (MBL) invites applications for a research specialist in the MBLs
Microscopy and Imaging Facility. Responsibilities include technical support and training for
specimen preparation, image acquisition, and computational processing and analysis using advanced
imaging techniques such as light sheet microscopy and scanning electron microscopy array tomography.
The successful candidate will receive training on these and other cutting-edge systems from their
respective instrument developers, commercial vendors, and resident staff.

This position is integral to MBLs strategic initiative to develop a Center for Imaging and Image
Analysis.

Key Areas of Responsibility:

* Support a broad variety of microscopy imaging projects and educational courses
* Help implement and refine novel imaging technologies
* Provide routine maintenance and troubleshooting on the imaging systems
* Provide training in specimen preparation and image processing and analysis
* Learn proper specimen preparation and image processing such as segmentation, particle tracking,
and shape analysis.
* Report to the Director Imaging Services within the Division of Research

Thank you,

Louie


Kerr|Director, Imaging Services; Staff Scientist

Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543
508-289-7273 |lkerr-at-mbl.edu {mailto:lkerr-at-mbl.edu} |www.mbl.edu {http://www.mbl.edu/}


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From: microscopy.listserver-at-gmail.com
Date: Fri, 1 Feb 2019 08:16:32 -0600
Subject: [Microscopy] viaWWW: Gatan EM Job Openings at SPIE Photonics West Job Fair 2019

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi.


Can anybody provide me with some upper limits for the charge density achievable in the focus of a focussed

electron (or ion) beam. The focal point doesn't have to be very small, 100 nm would be easily sufficient.

The speed of the electrons isn't important either.

Another interesting question is the largest possible convergence angle.

Regards.

Philip






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Title-Subject: [Filtered] EM Job Openings at SPIE Photonics West Job Fair 2019

Message: If you are attending SPIE Photonics West (Feb 5-6, 2019, Moscone Center, San Franisco, CA)
and looking for career opportunities in all EM fields, please stop by Table JF45 at the SPIE Job
Fair. Here are some of our current openings; also see www.gatan.com/careers:

California
Mechanical Design Engineer
Senior Systems Design Engineer
Technical Support Group Engineer Remote
Mechanical Design Engineer Drafter Temporary
Health Physicist
Sr. Applications Software Engineer
Technical Telemarketing Representative
Senior Reliability Engineer
Senior FPGA Design Engineer

Outside of California
Field Service Engineer Remote, Chicago
Field Services Engineer Remote, DC
Electronics Service Engineer Warrendale, PA
Sales Operations Administrator Shanghai, China
Sales Specialist UK
Field Services Engineer Korea
Manufacturing Product Engineer-Electronics Warrendale, PA

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From: microscopy.listserver-at-gmail.com
Date: Wed, 6 Feb 2019 13:25:00 -0600
Subject: [Microscopy] viaWWW: Oil diffusion pump oil

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Email: sina-at-uconn.edu Name: Sina Shahbbazmoahamdi

Organization: University of Connecticut/ REFINE Lab
Title-Subject: [Filtered] Postdoc position in Tomography and Machine learning

Message: Please contact me at sina-at-uconn.edu for postdoc and senior scientist positions in areas of
3D Xray microscopy, 3D FIB/SEM imaging and Machine learning.
Thanks
Sina
Sina Shahbazmohamadi, PhD
Assistant Professor
Director, REFINE lab website:refine.uconn.edu

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Email: pveril-at-uth.gr Name: Panagiotis Berillis

Organization: University of Thessaly

Title-Subject: [Filtered] Oil diffusion pump oil

Message: Dear all
In the lab we have a Philips CM10 electron microscope equipped with an EDWARDS oil diffusion pump.
We used to use Santovac 5 oil for this pump. Is there any other oil (Lion S e.g.) that we can use in
order to replace santovac 5?
Thank you Panagiotis Berillis

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From: microscopy.listserver-at-gmail.com
Date: Wed, 6 Feb 2019 13:25:38 -0600
Subject: [Microscopy] viaWWW: Employment Opportunity: FIB-SEM Scientist at the Naval

Contents Retrieved from Microscopy Listserver Archives
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Email: robert.morris-at-unnpp.gov Name: Robert Morris

Organization: Naval Nuclear Laboratory

Title-Subject: [Filtered] Employment Opportunity: FIB-SEM Scientist at the Naval Nuclear Laboratory
Message: NNL currently has an opening for a highly skilled Focused Ion Beam (FIB) microscopist. At
the Naval Nuclear Laboratory (NNL), we develop advanced naval nuclear propulsion technology,
ensuring the safety and reliability of our Navy's submarine and aircraft carrier fleets.

This individual will work in an advanced materials characterization laboratory and be responsible
for operating and maintaining 2 FIB/SEM systems (one FEI Quanta 3D FEG and a second system to be
procured and installed in 2020). Work will involve both radiological and non-radiological sample
analysis and will include micromechanical testing, electron backscatter diffraction (EBSD), energy
dispersive spectroscopy (EDS), site specific 3D feature analysis, and sample preparation for
transmission electron microscope (TEM) and atom probe tomography (APT) systems.
Candidate must meet US Citizenship requirements.

For more information, please visit the posting link:
https://navalnuclearlab.energy.gov/jobsearch/job-details/focused-ion-beam-fib-microscopist/22682/1/

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From: Philip.Koeck-at-ki.se
Date: Fri, 8 Feb 2019 00:55:14 -0600
Subject: [Microscopy] (electron microscopy) image of an electron beam

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Email: jpshield-at-uga.edu Name: John P Shields

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Title-Subject: [Filtered] UGA Bio TEM Workshop -

Message: There are still some spots available for our Biological TEM Workshop!

This intensive, three-day workshop provides a practical and basic theoretical introduction to the
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From johnsonloretta061zu-at-gmail.com Wed Feb 6 15:57:43 2019
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Has anybody ever recorded a transmission image of an electron beam using TEM, STEM or some holographic method?

All the best,

Philip


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From: Philip.Koeck-at-ki.se
Date: Fri, 8 Feb 2019 03:52:43 -0600
Subject: [Microscopy] (electron microscopy) clarification: image of an electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi again.


What I'm looking for is an actual image of an electron beam.
The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
Obviously this requires some sort of dual column setup.


All the best,


Philip


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From: cni-at-udel.edu
Date: Fri, 8 Feb 2019 08:48:12 -0600
Subject: [Microscopy] (electron microscopy) clarification: image of an electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

What's the point? Everhart did this in SEM about 50 years ago.
*******************************************************
Chaoying Ni, PhD
Director, W. M. Keck Center for Advanced Microscopy and Microanalysis
Professor, Materials Science and Engineering
http://www.camm.udel.edu; http://www.mseg.udel.edu
(302) 831-6359 (Phone); (302) 831-4545 (Fax)

Facility location: 154-174 Harker Laboratory
221 Academy Street, Newark, DE 19716

Mailing address:
University of Delaware
201 duPont Hall, Newark, DE 19716
*******************************************************

-----Original Message-----
X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se}
Sent: Friday, February 8, 2019 4:53 AM
To: Ni, Chaoying {cni-at-udel.edu}

Hi again.

What I'm looking for is an actual image of an electron beam.
The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
Obviously this requires some sort of dual column setup.

All the best,

Philip

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13, 66 -- Subject: RE: [Microscopy] (electron microscopy) clarification: image of an
13, 66 -- electron beam
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==============================End of - Headers==============================




From: John.Mardinly-at-asu.edu
Date: Fri, 8 Feb 2019 11:38:28 -0600
Subject: [Microscopy] Re: (electron microscopy) clarification: image of an

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

It’s called a Ronchigram.

A. John Mardinly, Ph.D., P.E.


} On Feb 8, 2019, at 2:53 AM, Philip.Koeck-at-ki.se wrote:
}
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} Hi again.
}
}
} What I'm looking for is an actual image of an electron beam.
} The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
} Obviously this requires some sort of dual column setup.
}
}
} All the best,
}
}
} Philip
}
}
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6, 136 -- From John.Mardinly-at-asu.edu Fri Feb 8 11:38:28 2019
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6, 136 -- From: John Mardinly {John.Mardinly-at-asu.edu}
6, 136 -- To: "Philip.Koeck-at-ki.se" {Philip.Koeck-at-ki.se} , MSA {Microscopy-at-Microscopy.com}
6, 136 -- Subject: Re: [Microscopy] (electron microscopy) clarification: image of an
6, 136 -- electron beam
6, 136 -- Thread-Topic: [Microscopy] (electron microscopy) clarification: image of an
6, 136 -- electron beam
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6, 136 -- electron beam
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6, 136 -- Date: Fri, 8 Feb 2019 17:46:25 +0000
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From: microscopy.listserver-at-gmail.com
Date: Sat, 9 Feb 2019 02:14:29 -0600
Subject: [Microscopy] viaWWW:JSM-5600LV Scan Rotation Issue

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Email: joshua.schwartz-at-niu.edu Name: Joshua Schwartz

Organization: Northern Illinois University

Title-Subject: [Filtered] JSM-5600LV Scan Rotation Issue

Message: Hello,

I believe I have an issue with scan rotation calibration. When the
image is in focus and the stage is moved, the image does not move in a
strait horizontal or vertical direction. The offset is about 3 degrees.
I have to under focus by about 10mm to have the image move strait. The
"Scan Rotation" button is off and I have never had to use it before.
Turing it on and adjusting it to 3 degrees adds some x motion if i am
moving y to correct the twist but this isn't precise enough to use stage
automation features.
Is there a way to adjust the scan rotation calibration on a 5600?
-Josh

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From: Philip.Koeck-at-ki.se
Date: Sat, 9 Feb 2019 02:41:43 -0600
Subject: [Microscopy] (electron microscopy) clarification: image of an electron beam

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I actually mean there should be two beams in the microscope, the electron beam you are imaging with and an additional electron or ion beam that you are taking an image of. Ideally the second beam would be orthogonal to the first. In a TEM this would mean sending in the second beam through the specimen port.

A ronchigram doesn't require two beams, does it?

All the best,

Philip
________________________________________
X-from: John Mardinly [John.Mardinly-at-asu.edu]
Sent: 08 February 2019 18:46
To: Philip Kck; MSA

Hi,

do you have a reference to this paper by any chance?

All the best,

Philip

________________________________________
X-from: Ni, Chaoying [cni-at-udel.edu]
Sent: 08 February 2019 15:56
To: Philip Köck; Microscopy-at-microscopy.com

Hi again.

What I'm looking for is an actual image of an electron beam.
The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
Obviously this requires some sort of dual column setup.

All the best,

Philip


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From: harvey.tyler.r-at-gmail.com
Date: Sun, 10 Feb 2019 10:03:12 -0600
Subject: [Microscopy] Re: (electron microscopy) clarification: image of an

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

“To see something” is fundamentally philosophical (remember the Schrodinger’s cat?)

When you use an orthogonal 2nd e-beam or any 2nd beam at any angle to shoot or watch the 1st beam, the resulting “1st“ beam will no longer be the 1st beam when it was not peeked, not to mention if such an experiment would even be possibly reliably realizable in the case of TEM.

In fact, Ronchigram is a good answer, and CBED can be another, I think. By choosing “different amorphous materials or crystals”, i.e. “2nd beams” or “instruments”, together with different focus and beam dose, the beam trajectory information can likely be extracted.

People also use Monte Carlo Methods

The reference is "T. Everhart, P. Hoff, J. Appl. Phys. 42, 5837 (1971)"

Curious at what you actually want to achieve. Can you share?

Good luck!
*******************************************************
Chaoying Ni, PhD
Director, W. M. Keck Center for Advanced Microscopy and Microanalysis
Professor, Department of Materials Science and Engineering
http://www.camm.udel.edu; http://www.mseg.udel.edu
(302) 831-6359 (Phone); (302) 831-4545 (Fax)

Facility location:
154-174 Harker Laboratory
221 Academy Street, Newark, DE 19716

Mailing address:
University of Delaware
201 duPont Hall, Newark, DE 19716
*******************************************************

-----Original Message-----
X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se}
Sent: Saturday, February 9, 2019 3:42 AM
To: Ni, Chaoying {cni-at-udel.edu}

Hi,

do you have a reference to this paper by any chance?

All the best,

Philip

________________________________________
X-from: Ni, Chaoying [cni-at-udel.edu]
Sent: 08 February 2019 15:56
To: Philip Köck; Microscopy-at-microscopy.com

Hi again.

What I'm looking for is an actual image of an electron beam.
The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
Obviously this requires some sort of dual column setup.

All the best,

Philip


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From irapier044ey-at-gmail.com Sat Feb 9 14:21:27 2019
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I think it's worth mentioning that this is basically what particle
colliders are, except the two beams aren't orthogonal, and people more
often use protons--so Phillip isn't crazy to ask. I couldn't quickly
find any paper where somebody reconstructed a beam profile based on
detected particles, but I'm sure someone has done that. The closest I
could quickly find was a paper where someone used braking radiation to
image a proton beam:
https://doi.org/10.1016/0029-554X(81)90734-5
Not quite what you're looking for, Phillip, but I'm sure it's there.
Seems that high-energy physicists are the right people to ask.
Tyler

On Sat, Feb 9, 2019 at 2:34 PM cni-at-udel.edu {cni-at-udel.edu} wrote:
}
}
}
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} “To see something” is fundamentally philosophical (remember the Schrodinger’s cat?)
}
} When you use an orthogonal 2nd e-beam or any 2nd beam at any angle to shoot or watch the 1st beam, the resulting “1st“ beam will no longer be the 1st beam when it was not peeked, not to mention if such an experiment would even be possibly reliably realizable in the case of TEM.
}
} In fact, Ronchigram is a good answer, and CBED can be another, I think. By choosing “different amorphous materials or crystals”, i.e. “2nd beams” or “instruments”, together with different focus and beam dose, the beam trajectory information can likely be extracted.
}
} People also use Monte Carlo Methods
}
} The reference is "T. Everhart, P. Hoff, J. Appl. Phys. 42, 5837 (1971)"
}
} Curious at what you actually want to achieve. Can you share?
}
} Good luck!
} *******************************************************
} Chaoying Ni, PhD
} Director, W. M. Keck Center for Advanced Microscopy and Microanalysis
} Professor, Department of Materials Science and Engineering
} http://www.camm.udel.edu; http://www.mseg.udel.edu
} (302) 831-6359 (Phone); (302) 831-4545 (Fax)
}
} Facility location:
} 154-174 Harker Laboratory
} 221 Academy Street, Newark, DE 19716
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} Mailing address:
} University of Delaware
} 201 duPont Hall, Newark, DE 19716
} *******************************************************
}
} -----Original Message-----
} X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se}
} Sent: Saturday, February 9, 2019 3:42 AM
} To: Ni, Chaoying {cni-at-udel.edu}
} Subject: [Microscopy] RE: (electron microscopy) clarification: image of an
}
}
}
}
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} Hi,
}
} do you have a reference to this paper by any chance?
}
} All the best,
}
} Philip
}
} ________________________________________
} X-from: Ni, Chaoying [cni-at-udel.edu]
} Sent: 08 February 2019 15:56
} To: Philip Köck; Microscopy-at-microscopy.com
} Subject: RE: [Microscopy] (electron microscopy) clarification: image of an electron beam
}
} What's the point? Everhart did this in SEM about 50 years ago.
} *******************************************************
} Chaoying Ni, PhD
} Director, W. M. Keck Center for Advanced Microscopy and Microanalysis Professor, Materials Science and Engineering http://www.camm.udel.edu; http://www.mseg.udel.edu
} (302) 831-6359 (Phone); (302) 831-4545 (Fax)
}
} Facility location: 154-174 Harker Laboratory
} 221 Academy Street, Newark, DE 19716
}
} Mailing address:
} University of Delaware
} 201 duPont Hall, Newark, DE 19716
} *******************************************************
}
} -----Original Message-----
} X-from: Philip.Koeck-at-ki.se {Philip.Koeck-at-ki.se}
} Sent: Friday, February 8, 2019 4:53 AM
} To: Ni, Chaoying {cni-at-udel.edu}
} Subject: [Microscopy] (electron microscopy) clarification: image of an electron beam
}
} ----------------------------------------------------------------------------
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} Hi again.
}
} What I'm looking for is an actual image of an electron beam.
} The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
} Obviously this requires some sort of dual column setup.
}
} All the best,
}
} Philip
}
}
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} 19, 112 -- Subject: RE: [Microscopy] (electron microscopy) clarification: image of an 19, 112 -- electron beam 19, 112 -- Thread-Topic: [Microscopy] (electron microscopy) clarification: image of an 19, 112 -- electron beam 19, 112 -- Thread-Index: AQHUv5U1R6eqGPvq9USSAnLoAmQvWqXV+rQwgAEuZ7c=
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} 34, 65 -- From cni-at-udel.edu Sat Feb 9 07:24:28 2019
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} 34, 65 -- 13:32:31 +0000
} 34, 65 -- From: "Ni, Chaoying" {cni-at-udel.edu}
} 34, 65 -- To: "'Philip.Koeck-at-ki.se'" {Philip.Koeck-at-ki.se} ,
} 34, 65 -- "'Microscopy-at-microscopy.com'"
} 34, 65 -- {Microscopy-at-microscopy.com}
} 34, 65 -- Subject: RE: [Microscopy] RE: (electron microscopy) clarification: image of an
} 34, 65 -- Thread-Topic: [Microscopy] RE: (electron microscopy) clarification: image of
} 34, 65 -- an
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} 34, 65 -- Date: Sat, 9 Feb 2019 13:32:31 +0000
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--
Dr. Tyler Harvey
Georg-August-Universität Göttingen
IV. Physical Institute
Friedrich-Hund-Platz 1
37077 Göttingen
Germany


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From: Philip.Koeck-at-ki.se
Date: Mon, 11 Feb 2019 02:23:47 -0600
Subject: [Microscopy] RE: (electron microscopy) clarification: image of an

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Dennis, Harvey, John, Alwyn, Ni, Reinhard and everybody following this.


There seems to be agreement that two beams will intersect each other at right angles seemingly unaffected since
the particle density in both is so small.

I'm thinking of the situation with a different model: I treat the "specimen beam" as a charge distribution along a line
(roughly speaking). As an example: For a beam of 1keV electrons with about 100 nA focused to about 1 micron
beam diameter I get a charge distribution corresponding to about 1 electron every 20 or so microns. This charge
distribution along a line produces an electrostatic potential which I approximate as smooth (on average).
The electron beam used for imaging this specimen, on the other hand, I describe as a monochromatic, plane wave.
This wave should be phase shifted as it travels through the potential. The phase shift is proportional to the projected
potential. The relative phase shift I get for 200 keV electrons is in the range of pi, but it's a very slowly varying spatial
distribution. To see this as an image would require a true phase contrast technique that has a good contrast transfer
at very low spatial frequencies. Maybe off-axis holography would work.
This fits very well with the observation that two beams visibly hardly influence each other. Most scattering is restricted to very small angles, since the phase shift varies very slowly. (I know I'm jumping around between the wave
and particle picture.)

Just to clarify: I'm not planning to do this experiment. I have something else in mind.

About the specimen beam current I mention (100 nA): It seems high, I guess, but I've been told this is easily achievable in e-beam lithography systems. I haven't found a paper confirming this, though.

Here's another interesting paper I got via a colleague on Research Gate:
https://arxiv.org/ftp/arxiv/papers/1610/1610.05602.pdf

All the best,

Philip


----------------------------------------------------------------------------

I think it's worth mentioning that this is basically what particle
colliders are, except the two beams aren't orthogonal, and people more
often use protons--so Phillip isn't crazy to ask. I couldn't quickly
find any paper where somebody reconstructed a beam profile based on
detected particles, but I'm sure someone has done that. The closest I
could quickly find was a paper where someone used braking radiation to
image a proton beam:
https://doi.org/10.1016/0029-554X(81)90734-5
Not quite what you're looking for, Phillip, but I'm sure it's there.
Seems that high-energy physicists are the right people to ask.
Tyler

On Sat, Feb 9, 2019 at 2:34 PM cni-at-udel.edu {cni-at-udel.edu} wrote:
}
} To see something is fundamentally philosophical (remember the Schrodingers cat?)
}
} When you use an orthogonal 2nd e-beam or any 2nd beam at any angle to shoot or watch the 1st beam, the resulting 1st beam will no longer be the 1st beam when it was not peeked, not to mention if such an experiment would even be possibly reliably realizable in the case of TEM.
}
} In fact, Ronchigram is a good answer, and CBED can be another, I think. By choosing different amorphous materials or crystals, i.e. 2nd beams or instruments, together with different focus and beam dose, the beam trajectory information can likely be extracted.
}
} People also use Monte Carlo Methods
}
} The reference is "T. Everhart, P. Hoff, J. Appl. Phys. 42, 5837 (1971)"
}
} Curious at what you actually want to achieve. Can you share?
}
} Good luck!
} *******************************************************
} Chaoying Ni, PhD
} *******************************************************
}
} -----Original Message-----
} ----------------------------------------------------------------------------
}
} Hi again.
}
} What I'm looking for is an actual image of an electron beam.
} The electron beam should be the specimen and probably orthogonal to the beam used for imaging it.
} Obviously this requires some sort of dual column setup.
}
} All the best,
}
} Philip


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From: gilpin-at-purdue.edu
Date: Wed, 13 Feb 2019 17:20:46 -0600
Subject: [Microscopy] help with stem FFT interpretation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

A lot of interest is beginning (here at UNMC) in the morphology of mitochondria under normal and experimental conditions. Now we all know that it is easy to get artifacts in mitochondria during fixation. Some of the questions from our various researchers involve looking for what I would consider slight or subtle changes. Things that any artifacts would complicate the interpretation of in the images.

So, I'm asking, does anyone out there have a fixative that will give preserved, artifact free mitochondria? If you have a favorite recipe, I would like you to share it with me. I should point out that the most likely type of fixation will be immersion fixation not perfusion.

Bonus question, can anyone explain what I would call the current fad, this interest in morphological changes in mitochondria? I'm sure we are not the only EM Lab getting these questions about mitochondria which seems to be an up and coming topic in medical research. All help is appreciated. Thank you.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu


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From ronaldfaust205xd-at-gmail.com Wed Feb 13 01:26:42 2019
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Dear Listers,

Could you, please, point me to the good and reliable source of secondary
antibodies conjugated to colloidal gold (5-10-15nm) in UK or Europe?

Previously, we used BBI a lot but it seems that now they have very
limited variety.

Thank you in advance!

Sincerely,

Alex

--
Dr. Aleksandr Mironov MD, PhD
Senior Experimental Officer
D.1527, M.Smith Building
EM Core Facility
School of Biological Sciences,
Faculty of Biology Medicine and Health
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

Tel. +44-(0)161-275-5645
E-mail: Aleksandr.Mironov-at-manchester.ac.uk
https://www.bmh.manchester.ac.uk/research/facilities/electron-microscopy/


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9, 31 -- From Aleksandr.Mironov-at-manchester.ac.uk Wed Feb 13 04:46:37 2019
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From ketcdutj170si-at-gmail.com Wed Feb 13 13:18:47 2019
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Hi everyone.

we have a strange result in an FFT of a hi res stem image.

We have a series of vertical lines that seem to be noise from somewhere. I can send the original image and/or the FFT if someone would be kind enough to offer to take a look!

Thanks!

​​​​​
Chris

Christopher J. Gilpin Ph.D.
Campus-wide Coordinator for Electron Microscopy
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
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gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
skype cjgilpin





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From: microscopy.listserver-at-gmail.com
Date: Thu, 14 Feb 2019 01:24:59 -0600
Subject: [Microscopy] viaWWW: negative staining and protein chemistry question

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-------- Forwarded Message --------


X-from: dlowry-at-asu.edu


This Question/Comment was submitted to the Microscopy Listserver
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Email: dlowry-at-asu.edu Name: David Lowry

Organization: Arizona State University

Title-Subject: [Filtered] negative staining and protein chemistry question

Message: A grad student working in our lab has encountered a problem
involving TEM negative staining of 2 different protein molecules. The
proteins are purified commercial products and are clearly distinct from
one another. In her experiments, the 2 proteins stain well (uranyl
acetate) and are easily resolved when they are applied individually to
glow-discharged grids.
However, in experiments when the 2 proteins are pre-mixed, one of them
acquires stain and is resolved normally, but the other one cannot be
resolved, it either does not stain correctly or does not bind to the
grid surface. The buffer used for all of her trials is the same (5 mM
HEPES, pH 7.2). We are looking for possible explanations for why this
may be occurring from list users who are knowledgeable of protein
chemistry
thank you-
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From: bethrichardson-at-uga.edu
Date: Thu, 14 Feb 2019 10:58:44 -0600
Subject: [Microscopy] vibratome schematics or help troubleshooting a problem

Contents Retrieved from Microscopy Listserver Archives
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Hi all,
Does anyone have the schematics for a Ted Pella vibratome 3000? If yes, can you send them to me?The vibratome is not advancing or retreatingnormally. It does advance and retreatbut super slowly and the normal noises it makes aren't happening. If you can troubleshoot this problem with me I would greatly appreciate it!
Ted Pella will not service it nor will Leica (who bought out Vibratome) and the quote from Southeast Pathology Services is more than we can afford at this time.
With your help I am ready to dissect this machine. SPS suggested that the barrel might be stuck. The foot pedal is not attached.
Have a Happy Valentine's Day,
Beth at Georgia Electron Microscopy, UGA, Athens, GA




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From: oshel1pe-at-cmich.edu
Date: Mon, 18 Feb 2019 10:31:32 -0600
Subject: [Microscopy] Any labs doing contract freeze-etch work?

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-------- Forwarded Message --------


X-from: Erico Freitas {ericotadeu-at-ufmg.br}


Dear all,


We've recently hosted a Plunge freezing Leica GP2, and I'm getting used
of it. We've got very high viscosity samples to do CryoTEM and I'm
wondering whether multiple blots would work better then single blot.

Has some if you have some idea about that?

Kind regards,
Erico Freitas {ericotadeu-at-ufmg.br}


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From gunnclar77uwnf-at-gmail.com Sun Feb 17 02:34:50 2019
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We have a project working on cyanobacteria and have discovered a need to do freeze-etch EM. We don't have the equipment to do this, so we are looking for a lab that can do freeze-etch on a contract basis.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
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989 774-7567 lab



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4, 89 -- Subject: Any labs doing contract freeze-etch work?
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From: microscopy.listserver-at-gmail.com
Date: Mon, 18 Feb 2019 19:08:41 -0600
Subject: [Microscopy] viaWWW:microwaving large samples in osmium

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Email: buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] microwaving large samples in osmium

Message: Hello. Does anyone have any experience with microwaving large
samples( 1.2 mm thick or larger) in osmium? I am wondering about time
and wattage to increase the penetration of osmium. So far I have only
tried 6 min at 400 w.
thanks in advance
JoAnn

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From: microscopy.listserver-at-gmail.com
Date: Mon, 18 Feb 2019 19:09:33 -0600
Subject: [Microscopy] viaWWW: Tips for TEM SAED JEOL 2100F

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Email: arunas.mesceriakovas-at-uef.fi Name: Arunas

Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F

Message: Hello,

I would like to ask some practical tips on performing SAED on a JEOL
2100F TEM. I am a complete newbie to TEM diffraction and the 2100 user
manual diffraction chapter is rather limited in describing influence of
various settings (or recommendation of it) on the outcome of the
diffraction patterns i.e. use of condenser lenses, alpha, spot values,
required/suggested alignment/correction procedures.
I usually (1)focus on the sample in question at about 100kx, (2) perform
voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then
switch to the SAED procedure in chapter 5.5.1 of the manual.

My samples are micron sized fly ash particles (silicates,
aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM
is operated at 200kV. So far one of the most notable problems I have is:
(1)After selecting/centering the area of interest on the fluorescent
screen (SA MAG mode, field limiting aperture inserted) I switch to SA
DIFF mode and the beam shifts position to the top left (midway radius)
on the fluorescent screen which makes navigation on the sample very
difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small
spot, the diffraction patterns do not appear completely spherical (mild
ellipse).


Any practical tips/steps on setting up the scope for the best SAED
experience would be greatly appreciated.

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From: microwink-at-gmail.com
Date: Mon, 18 Feb 2019 19:52:51 -0600
Subject: [Microscopy] Re: viaWWW: Tips for TEM SAED JEOL 2100F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

You are performing the basic procedures involved in selected area
diffraction just fine. Regarding the issues you are having, (1) I'm
not sure what you mean by the beam shifting when you switch to
diffraction mode. Is the area you are selecting in MAG mode physically
shifting outside the aperture (if so, you can adjust the image shifts
to correct this), or is the under/overfocused image simply shifting on
the green screen? To correct the later, turn on your projector shifts
(PLA on JEOL) and adjust the image back to the center of the green
screen.

(2)To correct astigmatism in the intermediate lenses, you'll need to
use your intermediate lens stigmator. On the JEOL, this is only
accessible through the alignment panel in the TEMCON software. You can
obtain the caustic spot in diffraction mode and correct using that, or
use an amorphous area of your sample.

Contact me directly if you need more detailed procedures.

Good luck,
Chris


On Mon, Feb 18, 2019 at 8:19 PM {microscopy.listserver-at-gmail.com} wrote:
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} Email: arunas.mesceriakovas-at-uef.fi Name: Arunas
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} Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F
}
} Message: Hello,
}
} I would like to ask some practical tips on performing SAED on a JEOL
} 2100F TEM. I am a complete newbie to TEM diffraction and the “2100 user
} manual” diffraction chapter is rather limited in describing influence of
} various settings (or recommendation of it) on the outcome of the
} diffraction patterns i.e. use of condenser lenses, alpha, spot values,
} required/suggested alignment/correction procedures.
} I usually (1)focus on the sample in question at about 100kx, (2) perform
} voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then
} switch to the SAED procedure in chapter 5.5.1 of the manual.
}
} My samples are micron sized fly ash particles (silicates,
} aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM
} is operated at 200kV. So far one of the most notable “problems” I have is:
} (1)After selecting/centering the area of interest on the fluorescent
} screen (SA MAG mode, field limiting aperture inserted) I switch to SA
} DIFF mode and the beam shifts position to the top left (midway radius)
} on the fluorescent screen which makes navigation on the sample very
} difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small
} spot, the diffraction patterns do not appear completely spherical (mild
} ellipse).
}
}
} Any practical tips/steps on setting up the scope for the best SAED
} experience would be greatly appreciated.
}
} Login Host: 193.167.228.180
} Listserver Email Form V - 20120416
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From: rcsencsits-at-belcan.com
Date: Tue, 19 Feb 2019 10:32:12 -0600
Subject: [Microscopy] RE: viaWWW: Tips for TEM SAED JEOL 2100F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear JoAnn,
it would be interesting to get info about the type of MW-apparatus (brand)
you are using....and how.....(water-load, intermittent on-off, etc.-, etc.).
Could you - please - tell "us" any experiences regarding ultrastructural
tissue preservation or obvious alterations due to your MW-procedere?

Unfortunately -in former times - I only sometimes used MW for (RAPID)
osmication of specimens (up to 4-6 mm) -
.... always with a waterload to prevent boiling of the OsO4-solution (I
promise: not only a really great mess if 'exploding' out from the vial but
also hazardous-especially to health...!) and just intermittently
irradiated for say 5-10x 1-2 seconds (rotary table/disk in action, full
power)

- when I tried to RAPIDLY process for diagnostic purposes...(but always
processed specimens also in parallel as usual by immersion into 1% buffered
OsO4-solution + permanent rotation of vials).

These times of practical experience or "doing osmication myself" were a long
time ago....

so:

(Full bibliographic info and abstracts in part added only for convenience of
reading....or choice...../.

you might read:
https://www.ncbi.nlm.nih.gov/pubmed/7730590
= https://journals.sagepub.com/doi/pdf/10.1177/43.5.7730590
J Histochem Cytochem. 1995 May;43(5):515-23.
Rapid primary microwave-aldehyde and microwave-osmium fixation: improved
detection of rat parotid acinar cell secretory granule alpha-amylase using a
post-embedding immunogold ultrastructural morphometric analysis.
Login GR1, Ku TC, Dvorak AM.
Author information
Abstract
Studies of methods for improved fixation are becoming increasingly important
in the field of quantitative immunocytochemistry. We used microwave
(MW)-assisted chemical fixation to show improved retention of salivary gland
acinar cell secretory granule alpha-amylase detected by a quantitative
immunogold method. Blocks (4-mm) of rat parotid gland were fixed by the
following methods: (a) MW irradiation in an aldehyde fixative (AF) for 6
sec; (b) immersion in AF for 1.5 hr; (c) MW irradiation in osmium tetroxide
(OT) for 9 sec; (d) immersion in OT for 1.5 hr; or (e) Sequential MW AF, 10
sec, MW OT rapid treatment (SMAORT), 10 sec. Specimens were processed
routinely for transmission electron microscopy. Thin sections of
Epon-embedded tissues were exposed first to rabbit IgG anti-human salivary
alpha-amylase and second to gold-conjugated goat anti-rabbit IgG. Granule
area was obtained by a point counting method. Labeling density was
calculated as the number of gold particles/micron 2 +/- SD. Specimens fixed
in seconds by MW-AF, MW-OT, or SMAORT showed ultrastructural preservation
similar to immersion fixation in AF or OT for 1.5 hr. Immunogold labeling
density of granule alpha-amylase was highest for SMAORT (874 microns 2)
compared to MW-AF (295 microns 2), MW-OT (248 microns 2), routine sequential
immersion in AF and OT (229 microns 2), or immersion in OT (no aldehyde)
(190 microns 2). This study establishes the improved retention of salivary
gland acinar cell secretory granule alpha-amylase and markedly enhanced
fixation speed for ultrastructural studies made possible by MW-chemical
fixation protocols that use aldehydes and osmium.
https://journals.sagepub.com/doi/pdf/10.1177/43.5.7730590


https://journals.sagepub.com/doi/pdf/10.1177/38.6.2335738
Rapid Primary Microwave-Osmium Fixation. I. Preservation of Structure for
Electron Microscopy in Seconds
GARY R.LOGIN,2 BARBARA K.DWYER, and ANN M.DVORAK
The Journal of Histochemistry and Cytochemistry Copyright1990 by The
Histochemical Society, Inc. Vol.38. No.6, pp.755-762, 1990
Microwave irradiation(MWIr) of tissues immersed in aldeydes has been used to
preserve fine structure in seconds. The purpose of this study was to extend
these findings to include rapid primary osmium fixation in a microwave (MW )
device with a high volume exhaust. Blocks of rat heart and liver were
trimmed to 4mm and exposed to 0.2M sym-collidine-buffered 2% osmium
tetroxide for a period of 6 -7 sec during MWIr (final solution temperature
~45 C).We also evaluated rapid fixation of tissues exposed to MWIr
simultaneously with immersion in dilute Karnovskys fixative (6-7 sec to ~
50C) followed by MWIr of spec imens immersed in osmium (7 sec to ~45 C]
.......
https://journals.sagepub.com/doi/pdf/10.1177/38.6.2335738



www.uic.umn.edu/sites/uic.umn.edu/files/mw_em_protocol_mas.pdf =
http://uic.umn.edu/sites/uic.umn.edu/files/mw_em_protocol_mas.pdf
Microwave Processing Protocol for Electron Microscopyusing the PELCO
BioWaveLaboratory Tissue Processing System
Getting Started
...
...
Osmium Tetroxide Fixation (see Fig. 1 for set-up)
Osmium Wattage Time
Vacuum
80W 2min. on - 2 min. off -
2min. on Yes
Cool to 20C and Repeat Time SequenceNotes:
1. Osmium is best used in concentrations of 1% or less. Aqueous works well
and is our choice.
2. Reduced osmium works well also (3% potassium ferricyanide mixed with an
equal volume of 2% osmium justbefore use).
3. Higher concentrations of osmium will retard penetration of fixative
into tissues in a microwave environment




https://pubs.acs.org/doi/abs/10.1021/om701099p?journalCode=orgnd7
Preparation of Ruthenium and Osmium Carbonyl Complexes Using Microwave
Heating: Demonstrating the Use of a Gas-Loading Accessory and Real-Time
Reaction Monitoring by Means of a Digital Camera
Nicholas E. Leadbeater* and Krista M. Shoemaker
Department of Chemistry, University of Connecticut, 55 North Eagleville
Road, Storrs, Connecticut 06269-3060
Organometallics, 2008, 27 (6), pp 12541258
DOI: 10.1021/om701099p
Publication Date (Web): February 22, 2008
Copyright 2008 American Chemical Society
* To whom correspondence should be addressed. E-mail:
nicholas.leadbeater-at-uconn.edu.


Berst wishes and regards,
and
Good luck !


Wolfgang


=============================================================
MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]

Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobil-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA
Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

Former Secretary and (until June2017) Board Member of the

SCUR
{The Society for Cutaneous Ultrastructure Research}
The Skin Imaging Society { www.scur.org }










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Email: buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] microwaving large samples in osmium

Message: Hello. Does anyone have any experience with microwaving large
samples( 1.2 mm thick or larger) in osmium? I am wondering about time and
wattage to increase the penetration of osmium. So far I have only tried 6
min at 400 w.
thanks in advance
JoAnn

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From janiceking866i-at-gmail.com Mon Feb 18 23:11:00 2019
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Arunas,
If your TEM is on service contract request a service visit and ask the engineer to demonstrate on a standard sample how they qualify that the TEM is functioning properly.

It is important that the beam and gun shifts and tilts are the same in imaging and diffraction modes (so the beam is where you think). This should be visible on a diagnostic page. On JEOL 2010 and older scopes it was pages 4 and 5 on the display. If alignments were done in "engineer mode" properly this should all be good. (I've been around 3 decades and have seen strange things; mistakes happen, people get distracted.)

In general I agree with the advice that Chris already provided.

Good luck,
Roseann

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Email: arunas.mesceriakovas-at-uef.fi Name: Arunas

Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F

Message: Hello,

I would like to ask some practical tips on performing SAED on a JEOL 2100F TEM. I am a complete newbie to TEM diffraction and the "2100 user manual" diffraction chapter is rather limited in describing influence of various settings (or recommendation of it) on the outcome of the diffraction patterns i.e. use of condenser lenses, alpha, spot values, required/suggested alignment/correction procedures.
I usually (1)focus on the sample in question at about 100kx, (2) perform voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then switch to the SAED procedure in chapter 5.5.1 of the manual.

My samples are micron sized fly ash particles (silicates, aluminosilicates). Fixed on holey carbon films on copper mesh. The TEM is operated at 200kV. So far one of the most notable "problems" I have is:
(1)After selecting/centering the area of interest on the fluorescent screen (SA MAG mode, field limiting aperture inserted) I switch to SA DIFF mode and the beam shifts position to the top left (midway radius) on the fluorescent screen which makes navigation on the sample very difficult. (2)Using the DIFF FOCUS knob to focus the beam into a small spot, the diffraction patterns do not appear completely spherical (mild ellipse).


Any practical tips/steps on setting up the scope for the best SAED experience would be greatly appreciated.




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From: microscopy.listserver-at-gmail.com
Date: Wed, 20 Feb 2019 17:14:52 -0600
Subject: [Microscopy] viaWWW:Resharpened knife quality

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Title-Subject: [Filtered] Resharpened knife quality

Message: Dear all,
We would like to know if anyone is experiencing any problems with
resharpened knives from Diatome. Thanks
Claudia


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From: debrat-at-bcm.edu
Date: Thu, 21 Feb 2019 10:09:29 -0600
Subject: [Microscopy] viaWWW:Resharpened knife quality

Contents Retrieved from Microscopy Listserver Archives
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Hello, Claudia,

YES, I have been having trouble with resharpened knives from Diatome!

I sent three knives to be resharpened in July of 2018 (resharpen 2, get one free). I received the first one back and a coworker began using it immediately. When I received the other two back, I found several knife marks on both knives and returned them both. I received a "new" knife in return, which I also found had knife marks. This was returned, and another one was sent. There were a few very faint marks on this one as well, but I was desperate and kept it. It seems to me that it is much more prone to damage from normal use than any knife I have had before, and I am very disappointed in it as well.

Thanks for the vent! I think I will try Microstar again.

Sharon



-----Original Message-----
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Sent: Wednesday, February 20, 2019 6:18 PM
To: Matthews,Sharon W {Sharon.Matthews-at-medicine.ufl.edu}

Oh My Dear Claudia,

Don't get me going on this subject. I am so disappointed in Diatome, a company I have been dealing with for 40+ years. The quality of their knives has declined over the years and I don't know why - maybe it's because they think they're the only game in town? I swapped an old knife and a considerable sum of money for a new one. There wasn't a single mm without scratches or knife marks on this "new" knife, and all sections showed chatter. I sent the knife back to them and, in their defense, they had another knife on my desk the following day. This knife seemed to be useful on the far left, but when I got to the middle of the blade, same stuff started happening - scratches and knife marks. I move to the far right and it was OK, but it looks like I'm stuck with a 3/4 of a knife. I don't have time to go 'round and 'round with Diatome.
Diatome used to be a great company, producing very high quality knives that were great across the entire edge. Not so much anymore. Tried Pella knives - they're even worse. Tried DDK - useless. Microstar is the only one left. I had a loaner knife when I first took this job and the only thing I didn't like about the Microstar knives was that the boat seemed really hydrophobic; other than that, the edge was darned good.
BTW, you should be hearing from my friend Lita, who works across the street at the Hughes Institute. She and I have been disappointed in Diatome for several years.

Best to you,
Debra Townley
Baylor College of Medicine

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Sent: Wednesday, February 20, 2019 5:17 PM
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Title-Subject: [Filtered] Resharpened knife quality

Message: Dear all,
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Claudia


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From: peter.heimann-at-uni-bielefeld.de
Date: Thu, 21 Feb 2019 13:29:27 -0600
Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
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Claudia
Same trouble ! (France)
We received a resharpened one" with many marks at the first use (on animal tissues embedded in Epoxy resin)
I sent them TEM images of the marks!
They told me that it was impossible that a such damaged diamant knife escaped the vigilance of the quality control of the Diatome factory!
We did not succeed to have a new one!
Best regards
Jeannine
________________________________________
De : debrat-at-bcm.edu {debrat-at-bcm.edu}
Envoy : jeudi 21 fvrier 2019 16:11
: Jeannine Lherminier
Objet : [Microscopy] RE: viaWWW:Resharpened knife quality

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Oh My Dear Claudia,

Don't get me going on this subject. I am so disappointed in Diatome, a company I have been dealing with for 40+ years. The quality of their knives has declined over the years and I don't know why - maybe it's because they think they're the only game in town? I swapped an old knife and a considerable sum of money for a new one. There wasn't a single mm without scratches or knife marks on this "new" knife, and all sections showed chatter. I sent the knife back to them and, in their defense, they had another knife on my desk the following day. This knife seemed to be useful on the far left, but when I got to the middle of the blade, same stuff started happening - scratches and knife marks. I move to the far right and it was OK, but it looks like I'm stuck with a 3/4 of a knife. I don't have time to go 'round and 'round with Diatome.
Diatome used to be a great company, producing very high quality knives that were great across the entire edge. Not so much anymore. Tried Pella knives - they're even worse. Tried DDK - useless. Microstar is the only one left. I had a loaner knife when I first took this job and the only thing I didn't like about the Microstar knives was that the boat seemed really hydrophobic; other than that, the edge was darned good.
BTW, you should be hearing from my friend Lita, who works across the street at the Hughes Institute. She and I have been disappointed in Diatome for several years.

Best to you,
Debra Townley
Baylor College of Medicine

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Email: lopezcl-at-ohsu.edu Name: Claudia Lopez

Organization: Oregon Health & Science University

Title-Subject: [Filtered] Resharpened knife quality

Message: Dear all,
We would like to know if anyone is experiencing any problems with
resharpened knives from Diatome. Thanks
Claudia


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From roddorva8uoea-at-gmail.com Thu Feb 21 12:07:03 2019
Return-Path: {roddorva8uoea-at-gmail.com}
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Dear All,

.... I am absolutely astonished by the (few) answers/comments from
"disappointed"  users of Diatome knifes.

I bought, inherited and used regularly and intensively new and
resharpened diamond knifes from Diatome ( TEM and semithin knifes) from
1975 to 2018 ( i.e. for over 40 years),

all in all I estimate that more than 20 knifes went through my hands and
to sum it up:  ALL were perfect without the smallest fault !

I can't understand the complaints when using a new knive in detecting
"bad" areas,  when I received a new or resharpened knive from Diatome I
immediately inspected the edge at the highest possible magnification at
my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s)
should have become visible in the "dark field" mode of its binocular,
and, as said, there were never any flaws in praxi ...

by the way: one time I privately bought at ebay-USA a "new and unused"
Diatome knive, when receiving it the seal of the storage box was broken
and the knife was dull and useless...

although by the way: all my (few) mail and phone contacts with Diatome
in Switzerland were positive and helpful.

greetings,

Peter Heimann

( Cell Biology, Univ. of Bielefeld, Germany;  retired in 2017 )



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From: bethrichardson-at-uga.edu
Date: Thu, 21 Feb 2019 14:49:38 -0600
Subject: [Microscopy] viaWWW:Resharpened knife quality

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Yes, I've also had lots of problems with Diatome diamond knives recently. We've had several resharpened and they produced knife marks and some areas on new knives are no better. I haven't complained to Diatome (but I should have done that). I, too, have been a loyal customer for 30+ years. It is sad to see the quality of their knives go to hell. They were always the best knives on the market. I now spend time apologizing to customers about the knife marks and re-section if necessary. I've even thought about making glass knives again (horrors!) but I'm too spoiled to go that route.

Beth Richardson,Georgia Electron Microscopy


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From: aheiss-at-amnh.org
Date: Thu, 21 Feb 2019 15:03:55 -0600
Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello all -- I don't have as much experience as many here (I've only been doing ultramicrotomy for 15 years or so) but my experience with Diatome knives has been the same as Peter Heimann's. I've used 3 Diatomes (all acquired new in different labs, the first probably in 2003 and the most recent in 2014), and they've all been flawless. The first of these was resharpened by them after years of use (in 2010, I think), and came back similarly flawless.

In a rare moment of more-than-adequate funding, I also acquired a MicroStar knife at the same time as the most recent Diatome. The quality of the MicroStar diamond edge has been just as good the Diatomes. However, the MicroStar's diamond was not set as precisely as any of the Diatomes. To be specific, the diamond is very slightly (a few micrometres) inset, such that in some cases the edges of the knife cannot be used without the specimen hitting the trough. (I apologise here: it's a bit hard to describe exactly how that works, but it is a problem.) The Diatomes I've used have never had this problem.

To be fair, I haven't contacted MicroStar about this issue; I don't know how common this problem is, or what their response would be. In my case, getting the problem fixed hasn't been necessary, since as long as I have two good diamond knives available, I want to use one for large survey sections (~1.2-1.5 mm wide, 70 nm thick) and one for much smaller and more delicate serial sections (~300 um wide, 50 nm thick). The MicroStar's fitting issues aren't a problem for survey-section work, while the Diatome's perfectly-set diamond is better for the serial work.

But most relevantly to this thread, and just to reiterate, I've had no reason to complain about Diatome knives, and have found them to be comparable in diamond and superior in setting to MicroStar's.


Aaron A. Heiss, Ph.D.
Research Scientist and Simons Foundation Fellow
Eunsoo Kim Laboratory
Department of Invertebrate Zoology
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024 USA

phone: 212-769-5838
fax: 212-769-5277
aheiss-at-amnh.org


________________________________________
X-from: peter.heimann-at-uni-bielefeld.de {peter.heimann-at-uni-bielefeld.de}
Sent: 21 February 2019 14:31
To: Aaron Heiss

Dear All,

.... I am absolutely astonished by the (few) answers/comments from
"disappointed" users of Diatome knifes.

I bought, inherited and used regularly and intensively new and
resharpened diamond knifes from Diatome ( TEM and semithin knifes) from
1975 to 2018 ( i.e. for over 40 years),

all in all I estimate that more than 20 knifes went through my hands and
to sum it up: ALL were perfect without the smallest fault !

I can't understand the complaints when using a new knive in detecting
"bad" areas, when I received a new or resharpened knive from Diatome I
immediately inspected the edge at the highest possible magnification at
my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s)
should have become visible in the "dark field" mode of its binocular,
and, as said, there were never any flaws in praxi ...

by the way: one time I privately bought at ebay-USA a "new and unused"
Diatome knive, when receiving it the seal of the storage box was broken
and the knife was dull and useless...

although by the way: all my (few) mail and phone contacts with Diatome
in Switzerland were positive and helpful.

greetings,

Peter Heimann

( Cell Biology, Univ. of Bielefeld, Germany; retired in 2017 )



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From: peter.heimann-at-uni-bielefeld.de
Date: Thu, 21 Feb 2019 15:24:52 -0600
Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

thanks Aaron for your comment!

indeed ( I did not comment on this) is the fine positioning of the
diamond within the aluminum trough and the resin "glue" perfect whereas
two other knifes from different maufacturers made problems in this
respect when trying to cut difficult positioned re-embedded specimen
blocks ( i.e. the block surface made contact with the resin cement
before contact with the diamond) ...

another (positive) characteristic point is that the knife edge of
Diatome knifes (at least in my hands) allowed for the water level to be
lowered extremely without "breaking away" or "ripping off" from the
knife edge and this pleasant feature was consistent over total life-time

best regards,

Peter ( Heimann )

#####################################

Am 21.02.2019 um 22:07 schrieb aheiss-at-amnh.org:
}
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} Hello all -- I don't have as much experience as many here (I've only been doing ultramicrotomy for 15 years or so) but my experience with Diatome knives has been the same as Peter Heimann's. I've used 3 Diatomes (all acquired new in different labs, the first probably in 2003 and the most recent in 2014), and they've all been flawless. The first of these was resharpened by them after years of use (in 2010, I think), and came back similarly flawless.
}
} In a rare moment of more-than-adequate funding, I also acquired a MicroStar knife at the same time as the most recent Diatome. The quality of the MicroStar diamond edge has been just as good the Diatomes. However, the MicroStar's diamond was not set as precisely as any of the Diatomes. To be specific, the diamond is very slightly (a few micrometres) inset, such that in some cases the edges of the knife cannot be used without the specimen hitting the trough. (I apologise here: it's a bit hard to describe exactly how that works, but it is a problem.) The Diatomes I've used have never had this problem.
}
} To be fair, I haven't contacted MicroStar about this issue; I don't know how common this problem is, or what their response would be. In my case, getting the problem fixed hasn't been necessary, since as long as I have two good diamond knives available, I want to use one for large survey sections (~1.2-1.5 mm wide, 70 nm thick) and one for much smaller and more delicate serial sections (~300 um wide, 50 nm thick). The MicroStar's fitting issues aren't a problem for survey-section work, while the Diatome's perfectly-set diamond is better for the serial work.
}
} But most relevantly to this thread, and just to reiterate, I've had no reason to complain about Diatome knives, and have found them to be comparable in diamond and superior in setting to MicroStar's.
}
}
} Aaron A. Heiss, Ph.D.
} Research Scientist and Simons Foundation Fellow
} Eunsoo Kim Laboratory
} Department of Invertebrate Zoology
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} phone: 212-769-5838
} fax: 212-769-5277
} aheiss-at-amnh.org
}
}
} ________________________________________
} X-from: peter.heimann-at-uni-bielefeld.de {peter.heimann-at-uni-bielefeld.de}
} Sent: 21 February 2019 14:31
} To: Aaron Heiss
} Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann )
}
}
}
}
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}
} Dear All,
}
} .... I am absolutely astonished by the (few) answers/comments from
} "disappointed"� users of Diatome knifes.
}
} I bought, inherited and used regularly and intensively new and
} resharpened diamond knifes from Diatome ( TEM and semithin knifes) from
} 1975 to 2018 ( i.e. for over 40 years),
}
} all in all I estimate that more than 20 knifes went through my hands and
} to sum it up: ALL were perfect without the smallest fault !
}
} I can't understand the complaints when using a new knive in detecting
} "bad" areas, when I received a new or resharpened knive from Diatome I
} immediately inspected the edge at the highest possible magnification at
} my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s)
} should have become visible in the "dark field" mode of its binocular,
} and, as said, there were never any flaws in praxi ...
}
} by the way: one time I privately bought at ebay-USA a "new and unused"
} Diatome knive, when receiving it the seal of the storage box was broken
} and the knife was dull and useless...
}
} although by the way: all my (few) mail and phone contacts with Diatome
} in Switzerland were positive and helpful.
}
} greetings,
}
} Peter Heimann
}
} ( Cell Biology, Univ. of Bielefeld, Germany; retired in 2017 )
}
}
}
} ==============================Original Headers==============================
} 12, 20 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 13:29:27 2019
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} 12, 20 -- Subject: Re: re-sharpened knife ( Peter Heimann )
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7, 22 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 15:24:51 2019
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7, 22 -- Subject: Re: [Microscopy] re-sharpened knife ( Peter Heimann )
7, 22 -- To: {aheiss-at-amnh.org} , {Microscopy-at-microscopy.com}
7, 22 -- References: {201902212107.x1LL7bQh030268-at-microscopy.com}
7, 22 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de}
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From: Rosemary.White-at-csiro.au
Date: Thu, 21 Feb 2019 15:35:19 -0600
Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann )

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi all,

Just to add - we have used new and resharpened Diatome knives for 30+ years with no complaint at all. We send our knives back to Diatome for resharpening through an excellent and reliable agent here in Australia.

I don't disbelieve the dissatisfied people, but I am surprised, this is quite different from our experience.

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
Canberra, ACT 2601
Australia

T 61 2 6246 5475
M 61 468 966 713
________________________________________
X-from: peter.heimann-at-uni-bielefeld.de [peter.heimann-at-uni-bielefeld.de]
Sent: Friday, 22 February 2019 8:25 a.m.
To: White, Rosemary (A&F, Black Mountain)

thanks Aaron for your comment!

indeed ( I did not comment on this) is the fine positioning of the
diamond within the aluminum trough and the resin "glue" perfect whereas
two other knifes from different maufacturers made problems in this
respect when trying to cut difficult positioned re-embedded specimen
blocks ( i.e. the block surface made contact with the resin cement
before contact with the diamond) ...

another (positive) characteristic point is that the knife edge of
Diatome knifes (at least in my hands) allowed for the water level to be
lowered extremely without "breaking away" or "ripping off" from the
knife edge and this pleasant feature was consistent over total life-time

best regards,

Peter ( Heimann )

#####################################

Am 21.02.2019 um 22:07 schrieb aheiss-at-amnh.org:
}
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} Hello all -- I don't have as much experience as many here (I've only been doing ultramicrotomy for 15 years or so) but my experience with Diatome knives has been the same as Peter Heimann's. I've used 3 Diatomes (all acquired new in different labs, the first probably in 2003 and the most recent in 2014), and they've all been flawless. The first of these was resharpened by them after years of use (in 2010, I think), and came back similarly flawless.
}
} In a rare moment of more-than-adequate funding, I also acquired a MicroStar knife at the same time as the most recent Diatome. The quality of the MicroStar diamond edge has been just as good the Diatomes. However, the MicroStar's diamond was not set as precisely as any of the Diatomes. To be specific, the diamond is very slightly (a few micrometres) inset, such that in some cases the edges of the knife cannot be used without the specimen hitting the trough. (I apologise here: it's a bit hard to describe exactly how that works, but it is a problem.) The Diatomes I've used have never had this problem.
}
} To be fair, I haven't contacted MicroStar about this issue; I don't know how common this problem is, or what their response would be. In my case, getting the problem fixed hasn't been necessary, since as long as I have two good diamond knives available, I want to use one for large survey sections (~1.2-1.5 mm wide, 70 nm thick) and one for much smaller and more delicate serial sections (~300 um wide, 50 nm thick). The MicroStar's fitting issues aren't a problem for survey-section work, while the Diatome's perfectly-set diamond is better for the serial work.
}
} But most relevantly to this thread, and just to reiterate, I've had no reason to complain about Diatome knives, and have found them to be comparable in diamond and superior in setting to MicroStar's.
}
}
} Aaron A. Heiss, Ph.D.
} Research Scientist and Simons Foundation Fellow
} Eunsoo Kim Laboratory
} Department of Invertebrate Zoology
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024 USA
}
} phone: 212-769-5838
} fax: 212-769-5277
} aheiss-at-amnh.org
}
}
} ________________________________________
} X-from: peter.heimann-at-uni-bielefeld.de {peter.heimann-at-uni-bielefeld.de}
} Sent: 21 February 2019 14:31
} To: Aaron Heiss
} Subject: [Microscopy] Re: re-sharpened knife ( Peter Heimann )
}
}
}
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}
} Dear All,
}
} .... I am absolutely astonished by the (few) answers/comments from
} "disappointed"� users of Diatome knifes.
}
} I bought, inherited and used regularly and intensively new and
} resharpened diamond knifes from Diatome ( TEM and semithin knifes) from
} 1975 to 2018 ( i.e. for over 40 years),
}
} all in all I estimate that more than 20 knifes went through my hands and
} to sum it up: ALL were perfect without the smallest fault !
}
} I can't understand the complaints when using a new knive in detecting
} "bad" areas, when I received a new or resharpened knive from Diatome I
} immediately inspected the edge at the highest possible magnification at
} my cutting machine ( Reichert Ultracut ), in my opinion any flaw(s)
} should have become visible in the "dark field" mode of its binocular,
} and, as said, there were never any flaws in praxi ...
}
} by the way: one time I privately bought at ebay-USA a "new and unused"
} Diatome knive, when receiving it the seal of the storage box was broken
} and the knife was dull and useless...
}
} although by the way: all my (few) mail and phone contacts with Diatome
} in Switzerland were positive and helpful.
}
} greetings,
}
} Peter Heimann
}
} ( Cell Biology, Univ. of Bielefeld, Germany; retired in 2017 )
}
}
}
} ==============================Original Headers==============================
} 12, 20 -- From peter.heimann-at-uni-bielefeld.de Thu Feb 21 13:29:27 2019
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} 12, 20 -- To: {Microscopy-at-microscopy.com}
} 12, 20 -- From: Peter Heimann {peter.heimann-at-uni-bielefeld.de}
} 12, 20 -- Subject: Re: re-sharpened knife ( Peter Heimann )
} 12, 20 -- Message-ID: {c807d7d6-6e27-1ade-ec6c-dfa0acc3148c-at-uni-bielefeld.de}
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From: duraine-at-bcm.edu
Date: Thu, 21 Feb 2019 16:03:42 -0600
Subject: [Microscopy] Re: Microscopy Resharpened Knife Quality

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


Hello all,

I would like to add my "official" comments on the knife matter. I am not upset with Diatome or EMS as they have always bent over backwards to make right any problem I've had, and most often to their vast expense. But back to the issue of diamond knives.

Yes I have had problems with Diatome knives from time to time, but with diligence Diatome has always re-sharpened or replaced my knives no problem. At one point a few months ago I still had very confusing problems with brand new knives being scored or damaged seemingly as opened or after using only one time. It looked like they were shipped to me that way. I even sent images to Diatome and EMS to show the scraping and marks I was getting. Helmut Gnaegi came all the way from Switzerland just to visit our lab and check the issues with the diamond knives we were experiencing.

It turned out after an hour or so of testing different samples and parameters, the clearance angle on my microtome was not accurate. The factory settings had been tweaked somehow. The clearance angle was no longer correct at the setting it showed. So instead of the knife clearance angle being 6 degrees, it was actually more like 3 degrees. That in itself would cause major scraping all along the knife and also damage the knife edge. We had to adjust the knife clearance angle up to 9 degrees in order for the water level and knife to cut properly. None of us would have thought that the clearance angle setting would be off. It's not something that I change. Helmut noticed it when he looked at the boat. The water was not level as it should be.

In the Leica UC7 microtome, the lighting is so good that I didn't even notice a difference in the water level lines. I was actually still able to cut at the wrong angle it just damaged the knife right away. Thereby making me think that I received damaged or insufficiently sharpened knives for months. Since Helmut's visit and the clearance angle revelation, I've had no other issues with the scraping or heavy knife marks. I am grateful to Helmut and to EMS for his visit. I relayed this information to others I knew too in the field. Whether they used it or not I don't know.

We work extensively with Drosophila tissue. The exoskeleton makes for us many problems in sectioning. So we know that we have to use Histo knives for making light slides, and only Ultra knives for TEM imaging. The two knives are sharpened differently it seems. We even have to request deeper sharpening at times which has helped. Diatome has had their own issues on the industrial level that affect manufacturing too. It's just the nature of this intricate business. For us, we cut up dead things, and dead things can be hard to deal with at times!

My advice to others is that if you are experiencing problems, take pictures, send micrographs, explain what is happening and systematically investigate the issue. Then perhaps an agreement can be made that will be profitable for both sides.

Thank you,

Lita Duraine
Certified TEM Specialist
HHMI- Molecular Genetics
Duncan Neurological Research Institute
Baylor College of Medicine
1250 Moursund St.
Houston, TX 77030
Office: 832-824-8704
MS: N1125.50



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From: tbargar-at-unmc.edu
Date: Thu, 21 Feb 2019 17:01:27 -0600
Subject: [Microscopy] Diatome knives

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I am surprised at the current thread of complaints about Diatome Diamond knives. I have been in EM since the early 1970's and have used many different brands of diamond knives, some of which are no longer around. When I took over my current facility in 2001, I had the resources to finally buy Diatome knives which I did in a heartbeat. They are the best. Here in the U.S. Stacie Kirsch owner of Electron Microscopy Sciences will make sure things are corrected, if you have a problem. I am not saying the people having bad experiences are to be discounted, but I hope they all have tried to work with the vendor. I also caution about airing complaints in a public forum such as the MSA listserver. Like any public medium these days one needs to be careful of what is said. I have asked for feedback on products, but have asked that people respond privately. Remember, what is said can be misconstrued and there is always two sides (sometimes more) to a story.

Tom Bargar
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
402-559-7347
tbargar-at-unmc.edu



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From: bethrichardson-at-uga.edu
Date: Thu, 21 Feb 2019 17:14:03 -0600
Subject: [Microscopy] Re: Microscopy Resharpened Knife Quality

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

My apologies to Diatome for my earlier post. I should have consulted with them as Lita Duraine did and worked out the knife issues. Her post was very helpful/informative. I will definitely test the knife angle on the ultramicrotome (it is never taken off 6 degrees).

Beth Richardson, Georgia Electron Microscopy


X-from: duraine-at-bcm.edu {duraine-at-bcm.edu}
Sent: Thursday, February 21, 2019 5:04 PM
To: Beth Richardson

Hello all,

I would like to add my "official" comments on the knife matter. I am not upset with Diatome or EMS as they have always bent over backwards to make right any problem I've had, and most often to their vast expense. But back to the issue of diamond knives.

Yes I have had problems with Diatome knives from time to time, but with diligence Diatome has always re-sharpened or replaced my knives no problem. At one point a few months ago I still had very confusing problems with brand new knives being scored or damaged seemingly as opened or after using only one time. It looked like they were shipped to me that way. I even sent images to Diatome and EMS to show the scraping and marks I was getting. Helmut Gnaegi came all the way from Switzerland just to visit our lab and check the issues with the diamond knives we were experiencing.

It turned out after an hour or so of testing different samples and parameters, the clearance angle on my microtome was not accurate. The factory settings had been tweaked somehow. The clearance angle was no longer correct at the setting it showed. So instead of the knife clearance angle being 6 degrees, it was actually more like 3 degrees. That in itself would cause major scraping all along the knife and also damage the knife edge. We had to adjust the knife clearance angle up to 9 degrees in order for the water level and knife to cut properly. None of us would have thought that the clearance angle setting would be off. It's not something that I change. Helmut noticed it when he looked at the boat. The water was not level as it should be.

In the Leica UC7 microtome, the lighting is so good that I didn't even notice a difference in the water level lines. I was actually still able to cut at the wrong angle it just damaged the knife right away. Thereby making me think that I received damaged or insufficiently sharpened knives for months. Since Helmut's visit and the clearance angle revelation, I've had no other issues with the scraping or heavy knife marks. I am grateful to Helmut and to EMS for his visit. I relayed this information to others I knew too in the field. Whether they used it or not I don't know.

We work extensively with Drosophila tissue. The exoskeleton makes for us many problems in sectioning. So we know that we have to use Histo knives for making light slides, and only Ultra knives for TEM imaging. The two knives are sharpened differently it seems. We even have to request deeper sharpening at times which has helped. Diatome has had their own issues on the industrial level that affect manufacturing too. It's just the nature of this intricate business. For us, we cut up dead things, and dead things can be hard to deal with at times!

My advice to others is that if you are experiencing problems, take pictures, send micrographs, explain what is happening and systematically investigate the issue. Then perhaps an agreement can be made that will be profitable for both sides.

Thank you,

Lita Duraine
Certified TEM Specialist
HHMI- Molecular Genetics
Duncan Neurological Research Institute
Baylor College of Medicine
1250 Moursund St.
Houston, TX 77030
Office: 832-824-8704
MS: N1125.50



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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Feb 2019 18:54:47 -0600
Subject: [Microscopy] viaWWW: Zeiss SEM Joystick Controller Lubricants

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Email: slockledge-at-tiptek.com Name: Scott P Lockledge

Organization: Tiptek, LLC

Title-Subject: [Filtered] Zeiss SEM Joystick Controller Lubricants

Message: Recently, we have experienced the joystick of a Zeiss SEM
controller "sticking" and not releasing. The Zeiss service
representative suggested trying a lubricant, but had no specific
suggestions. I found this one online to be used successfully by gamers:
Dow Corning Molykote 44 Medium Grease Lubricant.

Any experience with "sticking" joysticks, cleaning, or lubrication of
them? Any suggestions would be appreciated.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 Feb 2019 21:16:31 -0600
Subject: [Microscopy] viaWWW: Diamond knife discussion

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Email: leunissen-at-xtra.co.nz Name: Jan Leunissen

Organization: Aurion

Title-Subject: [Filtered] Diamond knife discussion

Message: Dear all,

I have been following the correspondence re diamond knife issues and I
feel the need to add a few words.
No doubt mistakes are made, by everyone, everywhere and all the time,
and like Rosemary White wrote, it is certainly not such that I
disbelieve the dissatisfied people". Being in business myself I value
criticism as it gives people a chance to seriously look into issues and
see where improvements can be made or how users/scientists can be
supported to get better results. Feedback is key. But also: negative
public messages can easily make a big dent in a companys reputation,
deservedly or not.
Diatome to almost all of us is Helmut Gnaegi, If there is one person in
business who really does everything he can to solve issues, to help
research projects, then I would have to say it is Helmut. As far as
experience is concerned: his 50th work anniversary with Diatome was just
3 weeks ago.

I admit, we do have some commercial interest in Diatome, since we
proudly represent them in The Netherlands but there is more than
business, there is also friendship and compassion.

I would urge everyone with questions or comments to contact Helmut, he
is very approachable and will leave no stone unturned.

Jan Leunissen
Dunedin, New Zealand

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From: sergei2-at-ornl.gov
Date: Fri, 22 Feb 2019 07:34:43 -0600
Subject: [Microscopy] Piezoresponse Force Microscopy and Nanoelectromechanics: video

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues

The 7 lecture course on Piezoresponse Force Microscopy and
Nanoelectromechanics is now available on the YouTube. The course is
based on tutorials given at the PFM workshops in } 20 locations worldwide
since 2006 (see https://pfm-workshop.weebly.com/ for partial
information, or https://www.facebook.com/PFM.Workshop.Network for more
recent updates), and includes the primers on PFM techniques developed at
the Center for Nanophase Materials Sciences (www.cnms.ornl.gov).

The course includes ~350+ slides and ~7 hours of recording, and
covers topics:

Lecture 1: Principles of PFM and electromechanics
https://youtu.be/UsyRW2_Kp-Y

Lecture 2: Voltage-dependent contact mechanics and resolution theory
https://youtu.be/BDmXUt4OOuY

Lecture 3: Dynamics in PFM
https://youtu.be/XKx1wSs4 {https://youtu.be/XKx1wSs4uXM} uXM
{https://youtu.be/XKx1wSs4uXM}

Lecture 4: PFM of ferroelectrics, polarization switching and patterning
https://youtu.be/mYeZQ8d3Mjk

Lecture 5: Switching spectroscopy PFM
https://youtu.be/53pqhCLURJg

Lecture 6: Advanced spectroscopic modes in PFM
https://youtu.be/y2yUhJoIKko

Lecture 7: PFM in liquid environments
https://youtu.be/HZI73NJCmrM

Share, like, and subscribe!

Sergei

--
Sergei V. Kalinin

Director,
Institute for Functional Imaging of Materials

Fellow MRS, IEEE, APS, Foresight Institute, IoP, AVS

Oak Ridge National Laboratory

Phone: (865) 241-0236


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From: microscopy.listserver-at-gmail.com
Date: Fri, 22 Feb 2019 15:38:43 -0600
Subject: [Microscopy] viaWWW: Zeiss SEM Joystick Controller Lubricants

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Whittaker, Scott {WHITTAKS-at-si.edu}

Scott,

I would say to be careful and use very sparingly as a "wet" lube will
just attract more dirt/dust which will end up further gumming things up.
In the past on an Amray I used a Teflon based dry lubricant and a
paraffin wax (not together). Have not had the issue on my Zeiss yet but
would do the same if it came up. We clean it and dust weekly to prevent
the problem and it seems to be working at least since 2011 when we
bought her. Considered a graphite on the Amray but was wary of
electrically bridging contacts as I didn't know how the controller
worked at the time.

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
Facebook | Twitter | Instagram



-----Original Message-----
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Sent: Thursday, February 21, 2019 8:01 PM
To: Whittaker, Scott {WHITTAKS-at-si.edu}




-------- Forwarded Message --------

X-from: slockledge-at-tiptek.com



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Email: slockledge-at-tiptek.com Name: Scott P Lockledge

Organization: Tiptek, LLC

Title-Subject: [Filtered] Zeiss SEM Joystick Controller Lubricants

Message: Recently, we have experienced the joystick of a Zeiss SEM
controller "sticking" and not releasing. The Zeiss service
representative suggested trying a lubricant, but had no specific
suggestions. I found this one online to be used successfully by gamers:
Dow Corning Molykote 44 Medium Grease Lubricant.

Any experience with "sticking" joysticks, cleaning, or lubrication of
them? Any suggestions would be appreciated.

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33, 54 -- Subject: Fwd: RE: [Microscopy] viaWWW: Zeiss SEM Joystick Controller
33, 54 -- Lubricants
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From: S.Walck-at-comcast.net
Date: Sun, 24 Feb 2019 12:00:38 -0600
Subject: [Microscopy] viaWWW: Tips for TEM SAED JEOL 2100F

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Allaz Julien {julien.allaz-at-erdw.ethz.ch}


Hi Erico,

CeB6 is a little bit more expensive than a LaB6, this would be the only
disadvantage I could think about… It is less sensitive to contamination
than LaB6, yet you will still need a clean ultra high-vacuum in the gun
(although not as low as a FEG). CeB6 has the advantage that it requires
a lower evaporation temperature and it has a lower work function (2.7 eV
for LaB6 vs 2.4 eV for CeB6). Therefore, you will get a longer lifetime
of CeB6 over LaB6, and a slightly better brightness.

If you can afford a CeB6, go for it! You can also contact Mike
Jercinovic at UMass Amherst (USA), he has more experience than I do with
LaB6 vs CeB6 - I actually got all the information listed above from him
when I was a postdoc at UMass Amherst :)

Julien


###########################
*Dr. Julien Allaz
*Head assistant for SEM/EPMA
Inst. für Geochemie und Petrologie
ETH Zürich
NW E 84
Clausiusstrasse 25
8092 Zürich
Switzerland

Tel: +41 44 632 37 20
Fax: +41 44 632 16 36
Email: julien.allaz-at-erdw.ethz.ch {mailto:julien.allaz-at-erdw.ethz.ch}
###########################

} On 21 Feb 2019, at 22:51, microscopy.listserver-at-gmail.com
} {mailto:microscopy.listserver-at-gmail.com} wrote:
}
}
}
}
} ----------------------------------------------------------------------------
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} -------- Forwarded Message --------
}
} Date: Thu, 21 Feb 2019 08:29:35 -0300
} X-from: Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} }
}
}
}
} Dear all,
}
}
} I would like to hear from the pros and cons of the cathode CeB6 in
} comparison to the LaB6?
}
}
} Kind regards,
}
} --
} Erico Freitas
}
} Physicist/Microscopist at Center of Microscopy
} Universidade Federal de Minas Gerais (UFMG)
} Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil.
} ZIP Code 31270-901.
} +55-31-3409-7573
} +55-31-3409-7575
}
} Coordinator:Transmission Electron Microscopy laboratory
}
} CV Lattes: *http://lattes.cnpq.br/8786127123101199*
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From solizbula031ioi-at-gmail.com Fri Feb 22 21:38:46 2019
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Arunas,

In addition to the HV alignment that you did, I am going to assume that you
did the whole alignment for the JEOL 2100F. If you don't have the full
alignment procedure, send me an email at scott dot d dot walck2 dot ctr at
mail dot mil and I will send you my alignment procedure that I require every
user to perform at the beginning of their session.

Follow these steps to get your SAED pattern on the phosphor screen(s):
1) In TEM mode without the SAD aperture in (you can also do this without the
Obj aperture in), hit the Std focus button.
2) Above 40kX (200kX is better), raise/lower the sample to get minimum
contrast (Your sample is now focused and the diffraction pattern is formed
in the back focal plane of the objective lens.) Use a live FFT to help you
find the minimum contrast.
3) Lower your TEM magnification to the desired value and center your sample
area.
4) Put in the appropriate SAD aperture and center it.
5) Turn the Brightness Knob fully clockwise. If you can read the JEOLS Hex
values, they should read "FFFF". Regardless, you will hear a beep when you
have turned it fully clockwise.
6) Press the SA DIFF button and select the desired cameral length.
7) Center the Transmitted spot with the PLA button selected and using the
Def controls. (This is the step that I think that you are missing in your
procedure.)
8) Focus the Diffraction spot with Diff Focus (while using the binoculars).
This is adjusting the Intermediate 1 lens to grab the back focal plane of
the objective lens for the projector lens system. The back focal plane is
where the diffraction pattern is formed.
9) If while focusing the IL1, you see astigmatism in the spot, correct it.
You have to select IL stig on the TEMCON screen, there is no button for it
on the control pads. Now this gets tricky. You have to turn the Dif Focus
button continuously through focus while adjusting first the X DEF control
and then the Y DEF control to get rid of the IL astigmatism while all the
time watching through the binoculars. You can look like a monkey while
doing it, so consider not having anybody around who might witness you doing
it.

This is actually the basic procedure for any microscope, not just the JEOL
2100F (other than the names of the specific controls.) You should have done
exactly this procedure one time for each camera length with your calibration
standard (gold or aluminum polycrystalline evaporated films) and saved them
in Digital Micrograph. You need to follow this procedure for every unknown
sample so that you have duplicated what was done for your standard at the
selected camera length. The key to success is reproducibility. You should
have about a 1-2% error or better.
Note: not only have I done this in DM for every camera length, I also use
Dave Mitchell's DiffTools script and have calibrated all of the camera
lengths in that as well. The good thing is that you can use the same
patterns for both.

Recording patterns using a digital camera:
10) If you are using a digital camera to record the pattern, lower the
exposure time to protect the camera.
11) In Digital Micrograph, draw two lines from corner to opposite corner to
find the center of the micrograph.
12) Put the controls in PLA mode and get ready to quickly center the
diffraction pattern by putting the transmitted spot at the center cross
13) Lift the screen and center the beam at the cross, QUICKLY. Then lower
the screen.
14) Now, use the beam block and center it while using the binoculars. Note:
if the beam block drifts on you, you need to call the service engineer and
have him tighten it so that it is stiff and takes effort to move it. That
way it will not move during collection of your diffraction pattern.
15) Lift the screen. If you are using DM and View with Bin3, put your
exposure at about 0.1 sec. You may have to adjust the beam block carefully
to make sure that any brightness around it is uniform on both sides. Do
this very carefully so that you don't expose the camera to the full spot.
16) Using the mouse, read the intensity of the brightest spots to find the
maximum intensity in the pattern by reading the values in DM.
17) Using the intensity that you found, increase the exposure until that
spot that you found is just under the saturation point of the camera.
16-17 alternative) Write a DM script that finds the maximum value in the
pattern and calculates the appropriate Record exposure. EASY TO DO.
18) Lower the exposure of the View to very low again.
19) Set your record exposure and take an image. Depending on what aperture
you have in, this can be a few seconds to more than 60 seconds. Avoid the
temptation to increase the brightness to get faster patterns. You want the
brightness fully clockwise because you want the beam as parallel as
possible.

This gives you a very good diffraction pattern image with the beam block in,
but you don't have the center of the pattern.

On older machines, it was easy to double expose a pattern a second time with
a shorter exposure and with the beam block out to record the transmitted
spot. It is a little more involved to do it with a digital camera.
20) While you are recording the long exposure in DM, change the Record
exposure to something like 0.02 to 0.08 s depending on how bright your
transmitted spot is.
21) When the recording is finished, quickly remove the beam block (that is
why you changed the exposure to a short one in the View) and press the
Record button to take another pattern with the short exposure.
22) When it is finished, immediately press the F6 button (this should be the
Beam Blank if it hasn't been changed from the default setting) to blank the
beam, lower the screen, and then press F6 again to un-blank the beam.
23) You have two patterns now. Save, but do not close the first one. You
have options how you want to use the second pattern with the transmitted
spot. You can find the X,Y coordinate with the maximum intensity and note
that on the first pattern. You can add the two patterns together. (If you
do this, then you also have to copy the tags from the first pattern to the
resultant image.) What I did was write a little script that selects and
copies a center square from the second image that includes the transmitted
spot and pastes it into the image of the first. I then add a "+" to the
name and save it in the directory where the first image was saved. With the
transmitted beam marked, it is very easy to center the pattern for
diffraction analysis software.

I hope this helps.
-Scott



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Email: arunas.mesceriakovas-at-uef.fi Name: Arunas

Title-Subject: [Filtered] Tips for TEM SAED JEOL 2100F

Message: Hello,

I would like to ask some practical tips on performing SAED on a JEOL 2100F
TEM. I am a complete newbie to TEM diffraction and the "2100 user manual"
diffraction chapter is rather limited in describing influence of various
settings (or recommendation of it) on the outcome of the diffraction
patterns i.e. use of condenser lenses, alpha, spot values,
required/suggested alignment/correction procedures.
I usually (1)focus on the sample in question at about 100kx, (2) perform
voltage centering alignment (HT WOB), (3) WOB Y/X correction. And then
switch to the SAED procedure in chapter 5.5.1 of the manual.

My samples are micron sized fly ash particles (silicates, aluminosilicates).
Fixed on holey carbon films on copper mesh. The TEM is operated at 200kV. So
far one of the most notable "problems" I have is:
(1)After selecting/centering the area of interest on the fluorescent screen
(SA MAG mode, field limiting aperture inserted) I switch to SA DIFF mode and
the beam shifts position to the top left (midway radius) on the fluorescent
screen which makes navigation on the sample very difficult. (2)Using the
DIFF FOCUS knob to focus the beam into a small spot, the diffraction
patterns do not appear completely spherical (mild ellipse).


Any practical tips/steps on setting up the scope for the best SAED
experience would be greatly appreciated.

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From: microscopy.listserver-at-gmail.com
Date: Mon, 25 Feb 2019 18:52:44 -0600
Subject: [Microscopy] viaWWW:retinal longitudinal sections with a glass knives

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Email: vakimler-at-oakland.edu Name: Vickie Kimler

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Title-Subject: [Filtered] Glass Knives

Message: Hi,
I am trying to cut retinal longitudinal sections with a glass knives on a brand new ultramicrotome.
I am using both 6.4 mm and 10 mm thick glass. I get chatter and shredding with 70-90 nm, although
the sections look better at 90 nm.
When I do semi-thins for LM, the viewing is fine with Toluidine blue O staining. Any suggestions?
Thanks!
Vickie

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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Feb 2019 08:40:32 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:retinal longitudinal sections with a

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-------- Forwarded Message --------
X-from: Common, Ralph {common-at-msu.edu}

As someone who has spent a lot of time sectioning retina I have to say the obvious -- get a diamond
knife.  It will make a huge difference.



On 2/25/2019 7:52 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: vakimler-at-oakland.edu Name: Vickie Kimler
}
} Organization: Oakland University
}
} Title-Subject: [Filtered] Glass Knives
}
} Message: Hi,
} I am trying to cut retinal longitudinal sections with a glass knives on a brand new ultramicrotome.
} I am using both 6.4 mm and 10 mm thick glass. I get chatter and shredding with 70-90 nm, although
} the sections look better at 90 nm.
} When I do semi-thins for LM, the viewing is fine with Toluidine blue O staining. Any suggestions?
} Thanks!
} Vickie
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Feb 2019 08:41:34 -0600
Subject: [Microscopy] viaWWW: Zeiss SEM Joystick Controller Lubricants

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Whittaker, Scott {WHITTAKS-at-si.edu}

Scott,

I would say to be careful and use very sparingly as a "wet" lube will just attract more dirt/dust
which will end up further gumming things up. In the past on an Amray I used a Teflon based dry
lubricant and a paraffin wax (not together). Have not had the issue on my Zeiss yet but would do the
same if it came up. We clean it and dust weekly to prevent the problem and it seems to be working
at least since 2011 when we bought her. Considered a graphite on the Amray but was wary of
electrically bridging contacts as I didn't know how the controller worked at the time.

Scott Whittaker
Lab Manager
Imaging
P.O. Box 37012 MRC-104 Room W150
Washington, DC 20013-7012
w 202.633.0891 whittaks-at-si.edu

SMITHSONIAN INSTITUTION
NATIONAL MUSEUM OF NATURAL HISTORY
Facebook | Twitter | Instagram



-----Original Message-----
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2019 8:01 PM
To: Whittaker, Scott {WHITTAKS-at-si.edu}




-------- Forwarded Message --------

X-from: slockledge-at-tiptek.com



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Email: slockledge-at-tiptek.com Name: Scott P Lockledge

Organization: Tiptek, LLC

Title-Subject: [Filtered] Zeiss SEM Joystick Controller Lubricants

Message: Recently, we have experienced the joystick of a Zeiss SEM controller "sticking" and not
releasing. The Zeiss service representative suggested trying a lubricant, but had no specific
suggestions. I found this one online to be used successfully by gamers: Dow Corning Molykote 44
Medium Grease Lubricant.

Any experience with "sticking" joysticks, cleaning, or lubrication of them? Any suggestions would
be appreciated.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Feb 2019 08:42:10 -0600
Subject: [Microscopy] Fwd: Re: LaB6 or CeB6?

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Allaz Julien {julien.allaz-at-erdw.ethz.ch}



Hi Erico,

CeB6 is a little bit more expensive than a LaB6, this would be the only disadvantage I could think
about… It is less sensitive to contamination than LaB6, yet you will still need a clean ultra
high-vacuum in the gun (although not as low as a FEG). CeB6 has the advantage that it requires a
lower evaporation temperature and it has a lower work function (2.7 eV for LaB6 vs 2.4 eV for CeB6).
Therefore, you will get a longer lifetime of CeB6 over LaB6, and a slightly better brightness.

If you can afford a CeB6, go for it! You can also contact Mike Jercinovic at UMass Amherst (USA), he
has more experience than I do with LaB6 vs CeB6 - I actually got all the information listed above
from him when I was a postdoc at UMass Amherst :)

Julien


###########################
*Dr. Julien Allaz
*Head assistant for SEM/EPMA
Inst. für Geochemie und Petrologie
ETH Zürich
NW E 84
Clausiusstrasse 25
8092 Zürich
Switzerland

Tel: +41 44 632 37 20
Fax: +41 44 632 16 36
Email: julien.allaz-at-erdw.ethz.ch {mailto:julien.allaz-at-erdw.ethz.ch}
###########################

} On 21 Feb 2019, at 22:51, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
} wrote:
}
}
}
}
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} -------- Forwarded Message --------
}
} Date: Thu, 21 Feb 2019 08:29:35 -0300
} X-from: Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} }
}
}
}
} Dear all,
}
}
} I would like to hear from the pros and cons of the cathode CeB6 in
} comparison to the LaB6?
}
}
} Kind regards,
}
} --
} Erico Freitas
}
} Physicist/Microscopist at Center of Microscopy
} Universidade Federal de Minas Gerais (UFMG)
} Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil.
} ZIP Code 31270-901.
} +55-31-3409-7573
} +55-31-3409-7575
}
} Coordinator:Transmission Electron Microscopy laboratory
}
} CV Lattes: *http://lattes.cnpq.br/8786127123101199*
} {https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Feb 2019 08:43:40 -0600
Subject: [Microscopy] Fwd: Re: Fwd: LaB6 or CeB6?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------


X-from: Erico Freitas {ericotadeu-at-ufmg.br}

Hi Warren,

Thanks for your reply.
We have been used LaB6 emmiter in our TEM Tecnai ST20. We used Denka and Kimball.
We had a look at the LaB6 and CeB6 specs in EMS website and found out they are pretty much they
same, but CeB6 has a lower vapour pressure, and perhaps a lower work funcion, and it might have a
longer life. But what puzzled us is that neither Kimball nor Denka produce CeB6, so we are wondering
about its quality, though we would like to give it a try. But want to hear few more opinions before
make our decision.

Regards,

On Thu, 21 Feb 2019, 19:10 Straszheim, Warren E [BIOTC], {wesaia-at-iastate.edu
{mailto:wesaia-at-iastate.edu} } wrote:

I have not used one, but I think it would be quite comparable. I am used to LaB6 filaments in
higher end research microscopes. I have only ever heard of CeB6 in desktop microscopes. CeB6
should be better than tungsten and maybe just a little worse than LaB6. If it is in a desktop
microscope, I would be more concerned about the other design details. How is the rest of the
column? How does it compare to a regular research microscope?

For our service lab, we run a field emitter on an FEI Quanta that is now rated at 1 nm
resolution. We routinely push 100kx (based on a 5-inch Polaroid reference). If you based it on
the image enlarged to the computer monitor, we would be pushing 300kx. How does that compare to
your spec?

Warren Straszheim

-----Original Message-----
From: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
[mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ]
Sent: Thursday, February 21, 2019 3:47 PM
To: Straszheim, Warren E [BIOTC]
Subject: [Microscopy] Fwd: LaB6 or CeB6?




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-------- Forwarded Message --------

Date:   Thu, 21 Feb 2019 08:29:35 -0300
X-from:         Erico Freitas {ericotadeu-at-ufmg.br {mailto:ericotadeu-at-ufmg.br} }



Dear all,


I would like to hear from the pros and cons of the cathode CeB6 in comparison to the LaB6?


Kind regards,

-- Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil.
ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}

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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 Feb 2019 08:44:32 -0600
Subject: [Microscopy] Fwd: Open position at IST Austria: Electron Microscope Operator (f/m)

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-------- Forwarded Message --------


X-from: Ludek LOVICAR {ludek.lovicar-at-ist.ac.at}


Electron Microscope Operator (f/m)
FULL TIME (40h)

IST Austria is a constantly growing international institute for conducting
frontier research in the life, physical, and formal sciences, located in
Klosterneuburg on the outskirts of Vienna in Austria.
Electron Microscopy facility at IST Austria is expanding. To strengthen our
team, we are looking for person, who will be highly motivated to take over
EM related responsibility in a challenging international environment.

Responsibilities
. Operating of EM and data acquisition by means of EMs (SEMs, S/TEMs).
. Monitoring of EMs, regular system check including systems alignment
and maintenance.
. Training, assistance, on-site support and supervision of users.
. Monitoring of compliance with rules.
. EM Image/data processing.
. Active contribution to the development of the EM facility.
Requirements
. Deep understanding of EM principle, image formation and practical
experience with S/TEM and SEM.
. Experience with tomography data collection and data processing.
. Experience with EDS (SEM & STEM) . Practical experience with FIB and/or cryo-EM applications is an
advantage.
. Knowledge of EM image/data processing.
. Excellent team player that can also work independently.
. High degree of reliability, organizational and interpersonal skills.
. Service oriented approach towards researches.
. Very good communication, presentation and writing skills in English.

To apply for this position send your application in one combined pdf
(including CV, certificates and references) by e-mail to:
recruiting-at-ist.ac.at
Contact
Ludek Lovicar
Manager of Electron Microscopy Facility
Phone: +43-(0)2243 9000 1066
Email: ludek.lovicar-at-ist.ac.at Website: www.ist.ac.at

=================================
Ludek Lovicar, PhD
Manager of Electron Microscopy Facility
Scientific Services Unit Institute of Science and Technology Austria
Am Campus 1
3400 Klosterneuburg
Austria

Phone: +43-(0)2243 9000 1066
Mobile: +43-(0)664 604 841 066
Fax: +43-(0)2243 9000-2000
Email: ludek.lovicar-at-ist.ac.at
Visit our website: www.ist.ac.at

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From: microscopy.listserver-at-gmail.com
Date: Wed, 27 Feb 2019 07:57:47 -0600
Subject: [Microscopy] viaWWW:Lehigh University Microscopy School 2019

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Email: slc6-at-lehigh.edu Name: Sharon Coe

Organization: Lehigh University

Title-Subject: [Filtered] Lehigh University Microscopy School 2019

Message: Now accepting registrations for the 49th annual Lehigh Microscopy School which will be held
on the campus of Lehigh University, Bethlehem, PA, June 2-7, 2019. All courses, lecturers and
instrumentation will be together for what promises to be a phenomenal week! Course offering
include: SEM and X-ray Microanalysis Introduction to SEM and EDS for the New Operator
Introduction to TEM (new Course) Transmission Electron Microscopy Focused Ion Beam:
Instrumentation and Applications Problem Solving: Interpretation and Analysis of SEM/EDS/EBSD
Data Quantitative X-ray Microanalysis: Problem Solving Using EDS and WDS Techniques. Register
and pay in full by April 12 for an early bird discount! Contact: Sharon Coe
(sharon.coe-at-Lehigh.edu) or Nikki Rump (nikki.rump-at-Lehigh.edu). See www.Lehigh.edu/microscopy for
registration form, prices, and details about courses, lecturers, and instrument suppliers.
Scholarships available for full-time graduate students.

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From: peter.miller-at-monash.edu
Date: Sun, 3 Mar 2019 23:41:08 -0600
Subject: [Microscopy] Re: Position available: FIB/SEM Manager at the Monash Centre for

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Email: tskrzypek-at-kul.pl Name: Tomasz

Organization: Laboratory of Electron Microscopy, Lublin

Title-Subject: [Filtered] EM 900 Zeiss

Message: Dear Colleagues, please help me with the problem of water flow in the Zeiss EM900
microscope. During work, a colleague felt the smell of burning and the message "check water"
appeared (red lamp). Flow and water pressure are fine. Probably it is about a sensor that has been
destroyed, maybe a short circuit on the board?
Please help and best regards.

Tomasz

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Title-Subject: [Filtered] AZtec 4.0

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From instyle-at-calone.ga Thu Feb 28 23:37:18 2019
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Reply-To: {dv.metus-at-gmail.com}

Join us for the webinar series on X-ray Computed Tomography for Materials Science to find out how X-ray CT can help your research.

When: Apr 3, 2019 11:00 AM Pacific Time
Part 1: X-ray Computed Tomography for Materials Science: Introduction

You will learn during the first webinar:
- What X-ray CT is
- How X-ray CT works
- How X-ray CT can be applied to materials science

The webinar series will cover image analysis and a number of application examples of food, pharmaceutical, composite materials etc.

To lean more, visit: https://www.rigaku.com/en/webinars/x-ray_ct_introduction

Recording of the webinar will be available for registered attendees.

Hope to see you there.

Aya Takase . Senior Scientist
Rigaku Americas Corporation
9009 New Trails Drive . The Woodlands, TX 77381 USA
T: 281-362-2300ex 208 . F: 281-364-3628




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From florjone348eriph-at-gmail.com Sat Mar 2 18:36:57 2019
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Greetings,

The Monash Centre for Electron Microscopy (MCEM, see
https://www.monash.edu/researchinfrastructure/mcem) is seeking to employ
an Electron Microscopist to manage and develop MCEM's focussed ion beam
capability and provide advanced expertise and training to support and
enable the work of researchers using the centre.

The closing date for applications is 2nd April.

For further information please see:

http://careers.pageuppeople.com/513/cw/en/job/588055/electron-microscopist


or contact Peter Miller.

Dr Peter Miller
Manager, Monash Centre for Electron Microscopy
10 Innovation Walk, Room G06
Clayton campus
Monash University
Victoria 3800
Australia

Phone: +61 3 9905 5291
Mobile: 0418 123 584
Email: peter.miller-at-monash.edu

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From: microscopy.listserver-at-gmail.com
Date: Tue, 5 Mar 2019 20:34:05 -0600
Subject: [Microscopy] viaWWW:blood in brain tissue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html




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Email: tong.wang-at-asrc.cuny.edu Name: Tong Wang

Organization: ASRC at GC of CUNY

Title-Subject: [Filtered] Research Faculty - Nanoscience Initiative - Advanced Science Research
Center in New York, New York

Message: Dear Colleagues,
The Nanoscience Initiative (nanoscience.asrc.cuny.edu) at the CUNY Advanced Science Research
Center (ASRC) invites applications for a full-time research faculty (open rank). Research Faculty
hold full-time, non-tenure track positions and may serve as principal or co-principal investigators
on grants or contracts, manage postdoctoral fellows and their research projects or supervise
graduate or undergraduate student research. While they may participate in instructional programs,
such as lectures or demonstrations, they are not assigned regular teaching duties.
For further information and/or application please see:

https://cuny.jobs/new-york-ny/research-assistant-associate-or-full-professor-nanoscience-initiative-advanced-science-research-center/c1fa26fde03b414db7192289d71c82cd/job/?utm_campaign=google_jobs_apply&utm_source=google_jobs_apply&utm_medium=organic

Best,

Tong

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Email: Buchsmith-at-gmail.com Name: JoAnn Buchanan

Organization: Allen Institute for Brain Science

Title-Subject: [Filtered] blood in brain tissue

Message: We have large pieces of human brain samples taken during surgery that need to be processed
for high contrast TEM. The remaining blood in the samples causes problems with the osmium and
thiocarbohydrazide reactions.Does anyone know what can be used to "quench" the blood so it becomes
less reactive.
thanks in advance
JoAnn

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From: microscopy.listserver-at-gmail.com
Date: Thu, 7 Mar 2019 08:50:05 -0600
Subject: [Microscopy] viaWWW: How to drying oils on Formvar

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Email: rvancamp-at-kettering.edu Name: R. Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Questions: How to drying oils on Formvar

Message: I am currently working with a few fatty acids as a component of samples. I am finding
oleic acid to be problematic and suspect I need to find a better way to dry the sample prior to
placing it in the TEM.

Formvar coated grids are usually prepared with a layer or two of KimWipe behind them placed on top
of my business card for additional moisture removal capacity, if necessary, and convenience in
handling the grid. Samples are deposited onto each grid by dispensing a few drops from a disposable
glass pipette (emphasize few). My practice is to place each grid in a desiccator cabinet for at
least 24 hours before it is examined in our TEM.

This is materials science research performed at 200 kV in a JEM-2100 Plus using a W-hairpin
filament. Beam current is ~15 uA.

I have observed sample vanish or, potentially grossly relocate, while attempting to focus on a
specimen. As I decreased magnification to scan for a new site I observed dark rings surrounding the
current specimen. I have never burned through a grid coating regardless of its composition so, I
suspect the oleic acid is interacting with the electrons and enabling some currently unknown
phenomena manifesting itself with the oleic acid or the acid/Formvar. Perhaps the heat deposited
into the oil is warming or deteriorating the Formvar in some way.

Please let me know if you have suggestions for treating these oily grids before they are placed in
the TEM.

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From: jbarkman-at-uoregon.edu
Date: Mon, 11 Mar 2019 10:44:46 -0500
Subject: [Microscopy] FIB-SEM Lab Manager Wanted

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X-from: JoAnn Buchanan {buchsmith-at-gmail.com}




Thank you everyone for the suggestions to use sodium metaperiodate or hydrogen peroxide to quench
the activity of blood cells during the processing. I am concerned that those chemicals may affect
the quality of the ultrastructure, especially the sodium metaperiodate.

I am processing large blocks- greater than i.2 mm in thickness for connectomics study. I need to
amplify the osmium staining and everything is done en bloc using THC to amplify the reduced osmium
staining. This is followed by UA and lead acetate en bloc. We are not doing any post staining
or immuno. Perhaps a low concentration of sodium peroxide will do the trick. I can try it on 1 or 2
of the slices and we can try to target the areas that have the clots.

Best wishes,
/JoAnn/




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Email: ldumas-at-tescan-usa.com Name: Laurent Dumas

Organization: TESCAN

Title-Subject: [Filtered] Looking for Application specialist

Message: Hello,
TESCAN USA is looking for FIB/SEM application specialist.
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From infoihioa-at-ns2.mgdots.co Mon Mar 11 03:47:07 2019
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The Center for Advanced Materials Characterization in Oregon (CAMCOR) at
the University of Oregon (UO) is looking for a new FIB-SEM Lab Manager.
The Focused Ion Beam-Scanning Electron Microscopy (FIB-SEM) Lab Manager
is involved in all activity within the FIB-SEM facility at the Center
for Advanced Material Characterization in Oregon (CAMCOR). This includes
providing professional technical management and services to support
shared equipment in the CAMCOR laboratories; user training and technical
support on SEM, FIB-SEM, and related equipment; overseeing instrument
upgrades and maintenance; assisting with course development and teaching
of laboratory components; providing analytical services for internal and
external clients; and maintaining and conducting facility tours and
demonstrations.

Review of applications begins on April 1, 2019 and will remain open
until the position is filled. More information about required and
preferred qualifications and working at UO can be found at:
http://careers.uoregon.edu/cw/en-us/job/523598/fibsem-lab-manager

-Julie


Julie Chouinard
CAMCOR
Surface Analytical and Microanalytical Facilities
1241 University of Oregon
Eugene, OR 97403
jbarkman-at-uoregon.edu
(541)-346-4580

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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Mar 2019 19:35:01 -0500
Subject: [Microscopy] viaWWW:LAMP1 antibody on HM20 resin

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Email: ralvaradojr-at-ufl.edu Name: Rudy

Organization: University of Florida
Title-Subject: [Filtered] LAMP1 antibody on HM20 resin

Message: Has anyone tried immunoEM labeling using LAMP1 antibody on HM20 resin? If you were
successful, can you please send me information from that antibody (e.g., brand, type, etc.). Thank you

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From: microscopy.listserver-at-gmail.com
Date: Mon, 11 Mar 2019 19:35:42 -0500
Subject: [Microscopy] viaWWW:SCSMM Spring Meeting

Contents Retrieved from Microscopy Listserver Archives
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Email: sergey-at-seas.ucla.edu Name: Sergey Prikhodko

Organization: UCLA

Title-Subject: [Filtered] SCSMM Spring Meeting

Message: Dear Fellow Microscopists: This is to inform you that Southern California Society for
Microscopy and Microanalysis (SCSMM) 2019 spring symposium is scheduled for Friday, April 12, 2019
at Cali2 Auditorium, UC Irvine campus. In the past decade microscopy and spectroscopy made major
advancements reflected in few recent Nobel Prizes in Chemistry: in 2008 for the discovery and
development of the green fluorescent protein; 2014 for the development of super-resolved
fluorescence microscopy; 2017 for the cryo electron microscopy and 2018 for the directed evolution
of enzymes. We are proud that among contributors to those great achievements are representatives of
California: Roger Y. Tsien (2008), Howard Hughes Medical Institute, University of California, San
Diego, La Jolla; William E. Moerner (2014) Stanford University, Stanford, CA; Frances H. Arnold
(2018) California Institute of Technology, Pasadena. We would like to mark those achievements and
are pleased that in our spring symposium we have contribution of the leading hubs representing life
sciences in California. We are proud to have Bruce Cohen from Lawrence Berkeley National Laboratory
(LBNL, Berkeley) as our 2019 MSA tour speaker. We also have Daniela Boassa from National Center for
Microscopy and Imaging Research (NCMIR, San Diego) and Mark Herzik from UCSD (San Diego). Please
register no later than 5 p.m. Friday, April 5th:
https://imri.uci.edu/content/2019-spring-meeting-registration#overlay-context
Check the Next Meeting updates on the website (www.scsmm.org). Annual regular membership is $25
(student $10) and this includes our Spring symposium and fall meeting. On-line registration is
required. At the symposium we will have both student platform talks and poster presentations. Please
send us your abstracts by March 15, 2019. Students are encouraged to contribute with presentation at
the SCSMM meeting. You'll get the chance 1) to win up to $500; 2) become a member of professional
society; 3) build up your network; 4) get your presentation at scientific meeting. This year we
continue to hold the SCSMM Image Contest. Please send us your images by April 6, 2019. We also have
a few prizes for the image contest. For more details, please see SCSMM website (www.scsmm.org) and
also follow SCSMM Facebook page.
We look forward to seeing you all at the symposium.
Sincerely,
SCSMM board
www.scsmm.org
https://www.facebook.com/microscopymicroanalysis


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From: steven.spurgeon-at-pnnl.gov
Date: Tue, 12 Mar 2019 16:29:52 -0500
Subject: [Microscopy] Registration Open for NexTEM 2019 Pre-Meeting Congress @ M&M 2019

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

We pleased to announce that registration is now open for the second annual Next-Generation Transmission Electron Microscopy (NexTEM) workshop, which will be held as a pre-meeting congress for Microscopy and Microanalysis 2019. This event will bring together researchers from diverse backgrounds to present the state-of-the-art in cutting edge electron microscopy tools and related applications. We are excited to again feature distinguished speakers from across the microscopy field.

Specific areas of interest include advanced detector designs for all states of matter, recent developments in electron and phonon optics/instrumentation, in situ and ultrafast TEM, cryo TEM, as well as data analytics and computational methods. Stimulating discussion and presentation of electron microscopy tools at the intersection of these fields will set the stage for advancement in these areas, as well as collaborative approaches to critical scientific issues in materials science, biology and medicine, physics, and chemistry.

Please visit the following link to register: https://www.microscopy.org/MandM/2019/program/congress.cfm

Workshop Topics Include:

Advanced Detector and Spectroscopy Developments
* Design and use of novel detectors to investigate material structure and functionality, including 4D STEM and ptychography.
* Vibrational and phonon spectroscopies at unprecedented spatial and energy resolution.
* Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples.
* Examination of materials structure and chemistry at cryogenic temperatures.

Frontiers of In Situ / Operando Microscopy
* Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids.
* Investigation of materials under stimulus across a range of sample environments and temperatures.
* New workflows for in situ experimentation to ensure reliability, reproducibility, and improve data quality.

Data-Driven Microscopy and Analysis
* Machine learning-based analysis of materials structure, dynamics, and defects.
* Integration of multiple large-scale imaging and spectroscopic data streams to elucidate physical descriptors of complex systems and phenomena.
* High-throughput simulation approaches to guide the interpretation of experimental datasets.

Confirmed Invited Speakers
* David Muller, Cornell University
* Naoya Shibata, University of Tokyo
* Stig Helveg, Haldor Topsoe
* Chongmin Wang, Pacific Northwest National Laboratory
* Quentin Ramasse, SuperSTEM
* Luiz Tizei, Université Paris Sud
* Paul Voyles, University of Wisconsin–Madison
* Hamish Brown, Lawrence Berkeley National Laboratory
* Rama Vasudevan, Oak Ridge National Laboratory


On behalf of the organizers, we look forward to seeing you in Portland!

Steven Spurgeon, Pacific Northwest National Laboratory
Demie Kepaptsoglou, SuperSTEM
Mitra Taheri, Drexel University

______________________________________
Steven R. Spurgeon, Ph.D.
Staff Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709
steven.spurgeon-at-pnnl.gov
www.stevenspurgeon.com
www.pnnl.gov




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16, 105 -- From: "Spurgeon, Steven R" {steven.spurgeon-at-pnnl.gov}
16, 105 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com}
16, 105 -- CC: "Taheri,Mitra" {mlt48-at-drexel.edu} ,
16, 105 -- Demie Kepaptsoglou
16, 105 -- {dmkepap-at-superstem.org}
16, 105 -- Subject: Registration Open for NexTEM 2019 Pre-Meeting Congress -at- M&M 2019
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16, 105 -- 2019
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From buviregi380boha-at-gmail.com Tue Mar 12 19:53:55 2019
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NIST PML (Physical Measurements Laboratory, Nanoscale Imaging Group) has an active research program on development of in situ scanning electron microscopy characterization and microfabrication of objects and devices in liquids and dense gaseous environments using environmental (micro-) fluidic (flow) cells equipped with electron transparent windows (including graphene). We have excellent experimental opportunities to conduct this research line including an array of dedicated SEM microscopes:
JEOL VP 7800 (equipped with: TTL SE, SED, BSE, transmitted electrons, ions detector, EDX, EBIC/EBAC, X-ray microtomography, cooling/ heating stages, flow cells etc), UHV Zeiss SEM (part of Omicron multiprobe UHV system) coupled with AES , XPS, FIB capabilities, Zeiss EVO coupled with micro-Raman spectrometer), clean room micro-fabrication facilities, in-lab sample preparation facilities (Leica 600 sputter/evaporator, AJA sputter/evaporator, UV/plasma cleaning/ashing and etc.

Currently, a postdoctoral position for highly motivated and experienced experimentalist is available.

US citizens are welcomed to explore the related NRC postdoctoral fellowship opportunity:
https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fnrc58.nas.edu%2FRAPLab10%2FOpportunity%2FOpportunity.aspx%3FLabCode%3D50%26ROPCD%3D506201%26RONum%3DB8512&data=02%7C01%7Candrei.kolmakov%40nist.gov%7C94654693292e41d2733208d6905af30e%7C2ab5d82fd8fa4797a93e054655c61dec%7C1%7C0%7C636855117643524885&sdata=PFtVSmxf%2BzSAGK%2B7hsz72bRhZznnSePJLqO83VWeEgI%3D&reserved=0

The preferable set of skills includes but not limited to:
liquid /atmospheric pressure SEM/TEM, electrochemistry, EBIC, XPS, AES, AFM, UHV surface science protocols, clean room experience, excellent writing and teamwork skills.
The letter of interest, CV, with a publication record and contact names of 3-4 references should be sent to Dr. Andrei Kolmakov, andrei.kolmakov-at-nist.gov

Thank you for your interest
_______________________________
Andrei Kolmakov Ph.D.
Project Leader, Nanoscale Imaging Group
Nanoscale Device Characterization Div.
Physical Measurements Laboratory,

NIST
100 Bureau Drive
Gaithersburg, MD 20899 MS 6204

Phone: (301) 975-4724
Fax: (301) 975-2303
https://www.nist.gov/people/andrei-kolmakov

_______________________________
Andrei Kolmakov Ph.D.
Project Leader, Nanoscale Imaging Group
Nanoscale Device Characterization Div.
Physical Measurements Laboratory,

NIST
100 Bureau Drive
Gaithersburg, MD 20899 MS 6204

Phone: (301) 975-4724
Fax: (301) 975-2303
https://www.nist.gov/people/andrei-kolmakov



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Email: whiteto-at-missouri.edu Name: Tommi White

Organization: University of Missouri Electron Microscopy Core

Title-Subject: [Filtered] Structural Electron Microsocpy: Missouri Symposium for Molecular Biophysics

Message: Dear Microscopy ListServer members

Please join us for the 3rd Missouri Symposium in Molecular Biophysics with a focus on "Structural
Electron Microscopy" on April 25-26th, 2019 at the University of Missouri?s Bond Life Sciences
Center. University of Missouri System anticipates building our facilities and efforts in the near
future in structural electron microscopy.
http://emc.missouri.edu/missourisymposium/

The Missouri Symposium will host many leaders in structural biology and electron microscopy. This
symposium is targeted both towards the life and material scientist to educate them on the
possibilities utilizing future electron microscopy investments and how they can further your
research interests.
On Thursday April 25th we will have a welcome dinner, participant poster session and keynote address
from Dr. Wah Chiu from the SLAC-Stanford CryoEM Center and Friday April 26th will include a day
filled with seminars from leaders in this emerging field. Please register in advance
($100/non-student; $50/student) and see our website for more details. We hope you can join us!
Below is a very brief summary of the speakers and a representative DOI-link of their work. Please
reach out to me with any questions.

Tommi
Tommi A. White, Ph.D.
Assistant Professor of Biochemistry
Director, Electron Microscopy Core Facility
University of Missouri
W117 Veterinary Medicine Building
1600 East Rollins Street
Columbia, MO 65211
573-882-8304
WhiteTo-at-missouri.edu
http://emc.missouri.edu


~~~~~~~~~~~~~
Wah Chiu (KEYNOTE) - For over 3 decades Dr. Chiu has lead methodology development for electron
cryo-microscopy. His work has made multiple transformational contributions in developing single
particle electron cryo-microscopy as a tool for the structural determination of molecular machines
towards atomic resolution (DOI:10.1016/j.molcel.2018.02.006).

Elizabeth Wright - has developed novel methods to perform correlative light and electron microscopy
at cryogenic temperatures, preserving ultrastructural details to visualize events occurring during
viral infection at the micro- and nanoscales, with light and electrons respectively
(DOI:10.1017/S1431927618012382).

Todd Yeates - The structure of a protein much smaller than 50 kDa can be successfully visualized
when it is attached to a large protein scaffold designed to hold 12 copies of the attached protein
in symmetric and rigidly defined orientation (DOI:10.1073/pnas.1718825115).

Tamir Gonen - pioneered the field of "Micro Electron Diffraction" that is garnering much interest
from numerous fields for quick high resolution structure determination of crystalline small
molecules as well as crystalline proteins (DOI:10.1021/acscentsci.8b00760).

Lena Kourkoutis - develops and applies novel electron microscopy techniques to advance the
fundamental understanding of materials and devices, extending the reach of aberration corrected STEM
to cryogenic temperature. Operating at low temperatures provides access to a broad range of
electronic phases that emerge during cooling of quantum materials and also allows the study of
processes that occur at liquid-solid interfaces (DOI:10.1038/s41586-018-0397-3).

Phoebe Stewart - using cryoEM to characterize targeted gene therapy and other nanoscale treatments
including viruses, viral/host factor complexes, engineered adenovirus-based vaccines, DNA
double-strand break repair complexes, and circadian clock protein complexes (DOI:10.1039/c6nr06948g).

Michael Stowell - studies how neurons communicate and their subsequent alteration upon disease using
structural methods (X-ray crystallography, cryoEM and tomography). Recently, he has determined the
structure of an ion transporter (DOI:10.1101/505453)

Jeffrey Lengyel - Lead Principal Scientist for Thermofisher Scientific (formerly FEI Company)
educating his customers on their microscopy tools and other related cutting-edge technologies
(DOI:10.1007/s10969-014-9179-9).


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From: Williams-at-GENECTR.HUNTER.CUNY.EDU
Date: Fri, 15 Mar 2019 17:30:41 -0500
Subject: [Microscopy] Cooling A Microscopy Room In Winter

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All:
I was wondering if any of you could help with an oft reoccurring problem we have in our facility.
We are unfortunate to be in a building where the heating is not well controlled in winter. The temperature our microscopes are in can vary from 60 - 80++ F (well roughly).
Ideally, we would like them to be at a temperature of around 74 F, and for that temperature to be as constant as possible.
We have managed this in the past by running window AC's but this is not a good solution as the temperature outside can be below freezing, and normal AC's don't work well in those conditions.
Opening a window or using a fan is not a good solution as we want the temperature to be stable.

I have looked at all season AC units, but these just seem to be regular ACs with heaters in them, they don't address the problem of how to keep the room cool when the building as a whole is hot and the outside weather is cold.

Suggestions.

Thanks
Lloyd Williams

Director Bio-Imaging & Network Core Facilities
Hunter College, City University of New York
Department of Biological Sciences
Rm. 826 HN
695 Park Ave
New York, NY 10065
212 650 3872; fax: 212 650-3656


Director Bio-Imaging & Network Core Facilities
Hunter College, City University of New York
Department of Biological Sciences
Rm. 826 HN
695 Park Ave
New York, NY 10065
212 650 3872; fax: 212 650-3656



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From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Mar 2019 19:36:58 -0500
Subject: [Microscopy] Fwd: Good source for used petrographic microscopes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

I wanted to bring the following post-doc position in my group to your
attention. If you are interested, please apply using this link:

https://jobs.uic.edu/job-board/job-details?jobID=111734&job=postdoctoral-research-associate-physics


--

Robert F Klie, PhD

Professor of Physics

Department of Physics
The University of Illinois at Chicago

845 W Taylor Street, M/C 273
Chicago, IL 60607
Tel: (312) 996-6064
Fax: (312) 996-9016

==============================Original Headers==============================
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From jeverett397ruvde-at-gmail.com Sun Mar 17 13:24:01 2019
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X-from: hlowers-at-usgs.gov


This Question/Comment was submitted to the Microscopy Listserver
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Email: hlowers-at-usgs.gov Name: Heather Lowers

Organization: USGS

Title-Subject: [Filtered] Quantitative Microanalysis 2019

Message: *** Apologies for cross-posting ***

Quantitative Microanalysis 2019 will be held at the University of Minnesota, Minneapolis June
24-27th. This four day MAS Topical Conference consists of user group meetings followed by a three
day plenary meeting covering quantitative microanalysis for beginners to experts, and focusing on
EPMA, SEM, and advances in microanalysis. The conference includes tutorials, invited and contributed
presentations, laboratory demos, and group discussion.

https://the-mas.org/events/topical-conferences/qma-2019/

Our invited speakers to date include:
Ben Buse (University of Bristol, UK): Overview of EPMA-WDS and field emission gun EPMA.
John Donovan (Probe Software, USA): Compositional mapping.
Karsten Goemann (University of Tasmania, Hobart, Australia): Fine tuning SEM for quantitative
analysis and combined EDS-WDS
Mike Jercinovic (University of Massachusetts, Amherst, USA), EPMA trace element analysis
Colin MacRae (CSIRO Mineral Resources, Clayton, Australia): Cathodoluminescence and hydrated mineral
analyses
William Nachlas (Syracuse University, USA): Standards and reference materials

Early Career Scholar student financial support deadline has been extended to April 1. Reimbursement
will be prioritized for those students and early career professionals who submit an abstract for a
platform or poster presentation prior to April 1 followed by those students attending the topical
conference.

Early bird registration for professionals ends March 31. Register before rates go up!

Look forward to seeing you at QMA 2019!

The QMA 2019 committee

Login Host: 136.177.22.13
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From trojlita384fagov-at-gmail.com Tue Mar 19 13:38:36 2019
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-------- Forwarded Message --------

X-from: Beavers, Roy {rbeavers-at-mail.smu.edu}



Looking for good companies that deal in used petrographic microscopes.

Prefer Nikon or Zeiss scopes that can accommodate high resolution cameras.



*Roy Beavers*

Southern Methodist University

Department of Earth Sciences

3225 Daniel Ave

Dallas TX 75205

Voice: 214-768-2756

Email: rbeavers-at-smu.edu


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From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Mar 2019 19:37:38 -0500
Subject: [Microscopy] Fwd: Help finding a wax, glue, or M-bond that works at room

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


X-from: Rosa Diaz {rdiazri-at-purdue.edu}




Hello,

I'm working with a sample that is really sensitive to temperatures above room temperature.

I need to polish the sample to make a wedge sample for TEM imaging. I need to glue the sample onto a
polisher holder, however i need to find a M-bond, wax, or glue that can be cured at room temperature
and will be hard enough so the sample stays in the holder while polishing! Also, easy to be removed
with a simple solvent.

The sample is a InSb (semiconductor) substrate. Really brittle and soft as well.


Please let me know if you know of any product and thanks in advance.

Rosa

/─────────//─────────//─────────//───────//─/
/Rosa E. Diaz, PhD/
/Electron Microscopy Research Scientist /
/Birck Nanotechnology Center/
/Purdue University/
/1205 W. State Street, West Lafayette, IN 47907-2057 /
/Office: BRK-1272  Tel ://765-496-1075/
/─────────//─────────//─────────//───────//─/

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From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Mar 2019 19:39:37 -0500
Subject: [Microscopy] viaWWW: Critical Point Dryer Problem

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X-from: becks-at-ncc.edu


This Question/Comment was submitted to the Microscopy Listserver
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Email: becks-at-ncc.edu Name: Stephen Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Critical Point Dryer Problem

Message: Dear Colleagues,

I have just received a Tousimis 931 critical point dryer to replace a vintage 1992 Polaron Jumbo
CPD. I am having problems with residual ethanol in the chamber (large 3.5 diameter) after the
completion of the critical point process. Samples are therefore wet and subject to surface tension
distortion/collapse.
Has anyone else had a similar problem? I have been working with the company tech support and have
verified my liquid CO2 tank volume and syphon tube, increased the AUTO purge time from 10 to 15
minutes and verified the metering valve factory settings.
Any suggestions are appreciated!

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy Department of Biology
Nassau Community College
Garden City, NY 11530

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From: microwink-at-gmail.com
Date: Tue, 19 Mar 2019 19:55:24 -0500
Subject: [Microscopy] Re: Fwd: Help finding a wax, glue, or M-bond that works

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Rosa,

I've had good luck using Loctite 460 super glue. It cures at room
temperature in seconds or minutes and can be removed by soaking sample
and stub in acetone. Shear strength should be sufficient for
tripod/wedge polishing; if not, there is a Loctite 454 super glue
that's a bit stronger in shear (from what I understand).

Good luck with your InSb sample. That's not a fun material system to polish.

Cheers,
Chris


On Tue, Mar 19, 2019 at 8:49 PM {microscopy.listserver-at-gmail.com} wrote:
}
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} -------- Forwarded Message --------
}
}
} X-from: Rosa Diaz {rdiazri-at-purdue.edu}
}
}
}
}
} Hello,
}
} I'm working with a sample that is really sensitive to temperatures above room temperature.
}
} I need to polish the sample to make a wedge sample for TEM imaging. I need to glue the sample onto a
} polisher holder, however i need to find a M-bond, wax, or glue that can be cured at room temperature
} and will be hard enough so the sample stays in the holder while polishing! Also, easy to be removed
} with a simple solvent.
}
} The sample is a InSb (semiconductor) substrate. Really brittle and soft as well.
}
}
} Please let me know if you know of any product and thanks in advance.
}
} Rosa
}
} /─────────//─────────//─────────//───────//─/
} /Rosa E. Diaz, PhD/
} /Electron Microscopy Research Scientist /
} /Birck Nanotechnology Center/
} /Purdue University/
} /1205 W. State Street, West Lafayette, IN 47907-2057 /
} /Office: BRK-1272 Tel ://765-496-1075/
} /─────────//─────────//─────────//───────//─/
}
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From: Rosemary.White-at-csiro.au
Date: Tue, 19 Mar 2019 20:03:43 -0500
Subject: [Microscopy] Re: viaWWW: Critical Point Dryer Problem

Contents Retrieved from Microscopy Listserver Archives
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Hi Steve,

I'd recommend trying a considerably longer purge time. We have an older Tousimis 815 with a smaller chamber (1.25 inches), and our standard purge time is 20 min, extended to 30-35 min for larger samples. By "large" I mean maybe 60x40x30 mm dimensions. It does mean you chew through the liquid CO2, but better than losing precious samples.

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1700
Canberra, ACT 2601
Australia

T 61 2 6246 5475
E rosemary.white-at-csiro.au


On 20/3/19, 11:40 am, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:




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Email: becks-at-ncc.edu Name: Stephen Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Critical Point Dryer Problem

Message: Dear Colleagues,

I have just received a Tousimis 931 critical point dryer to replace a vintage 1992 Polaron Jumbo
CPD. I am having problems with residual ethanol in the chamber (large 3.5” diameter) after the
completion of the critical point process. Samples are therefore wet and subject to surface tension
distortion/collapse.
Has anyone else had a similar problem? I have been working with the company tech support and have
verified my liquid CO2 tank volume and syphon tube, increased the AUTO purge time from 10 to 15
minutes and verified the metering valve factory settings.
Any suggestions are appreciated!

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy Department of Biology
Nassau Community College
Garden City, NY 11530

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From: microscopy.listserver-at-gmail.com
Date: Tue, 19 Mar 2019 20:12:19 -0500
Subject: [Microscopy] Fwd: RE: Fwd: Good source for used petrographic

Contents Retrieved from Microscopy Listserver Archives
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X-from: Bart Cannon {cannonmp-at-comcast.net}




Keep checking on eBay.  I’ve seen great petrographic and ore microscopes made by Leitz and Zeiss for
$1,500.  And don’t overlook your local university surplus auctions.   The parts for all these scopes
will be available for decades via surplus inventories.

Sent from Mail {https://go.microsoft.com/fwlink/?LinkId=550986} for Windows 10

*From: *microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
*Sent: *Tuesday, March 19, 2019 5:49 PM
*To: *cannonmp-at-comcast.net {mailto:cannonmp-at-comcast.net}
*Subject: *[Microscopy] Fwd: Good source for used petrographic microscopes

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X-from:                 Beavers, Roy {rbeavers-at-mail.smu.edu}

Looking for good companies that deal in used petrographic microscopes.

Prefer Nikon or Zeiss scopes that can accommodate high resolution cameras.

*Roy Beavers*

Southern Methodist University

Department of Earth Sciences

3225 Daniel Ave

Dallas TX 75205

Voice: 214-768-2756

Email: rbeavers-at-smu.edu

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19, 53 -- Subject: Fwd: Good source for used petrographic microscopes

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From: benada-at-biomed.cas.cz
Date: Wed, 20 Mar 2019 03:16:58 -0500
Subject: [Microscopy] Re: viaWWW: Critical Point Dryer Problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Steve,
We had similar problem some years ago, when we have to replace our old
Balzers 010 CPD unit. In the new unit there was a minor leak in one
valve. We find it out, after careful searching of the drying process.
Due to the leak the pressure in the chamber was just bellow or
oscillating around the critical pressure value. The unit has an analog
pressure meter and it was hard to see it on the analog meter. After
replacement of the leaky fitting, all is working fine.

Regards

Oldrich

--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Tue, 19 Mar 2019 19:40:12 -0500, microscopy.listserver-at-gmail.com
wrote :
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} Email: becks-at-ncc.edu Name: Stephen Beck
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} Organization: Nassau Community College
}
} Title-Subject: [Filtered] Critical Point Dryer Problem
}
} Message: Dear Colleagues,
}
} I have just received a Tousimis 931 critical point dryer to replace a
} vintage 1992 Polaron Jumbo CPD. I am having problems with residual
} ethanol in the chamber (large 3.5_ diameter) after the completion of
} the critical point process. Samples are therefore wet and subject to
} surface tension distortion/collapse. Has anyone else had a similar
} problem? I have been working with the company tech support and have
} verified my liquid CO2 tank volume and